KR20200080500A - Cosmetic composition for anti-wrinkle activity comprising fermented soybean paste and sea weed extracts as active ingredient - Google Patents

Abstract

The present invention provides a cosmetic composition excellent in anti-wrinkle effect by containing fermented soybean paste, phellinus linteus powder, dietary fiber powder, and seaweed extract as active ingredients.

Description

Cosmetic composition for anti-wrinkle activity comprising fermented soybean paste and sea weed extracts as active ingredient}

The present invention relates to a cosmetic composition for preventing wrinkles comprising miso and seaweed extract as an active ingredient.

The skin consists of two layers, the epidermis and the dermis. The outer layer is the epidermis, which is mainly composed of keratinocytes, melanocytes, and Langerhans cells, whose basic function is to maintain body moisture, barrier against harmful chemicals and pathogens, and cell renovation. Is to carry out the process. The inner layer is the dermis, which is composed of fibroblasts, adipocytes, and macrophages and is tightly coupled to the epidermal layer through the basement membrane, and contains numerous nerve endings that sense touch and temperature. In addition, the dermis contains hair follicles, sweat glands, sebaceous glands, apocrine glands and blood vessels, and one of the main functions of the dermis is to maintain the elasticity and appearance of the skin.

In addition, the dermis contains an extracellular matrix, which is formed of a group of extracellular proteins (fibrous proteins, glycoproteins and proteoglycans), the main possible being to maintain the skin structure. The two most important fibrous proteins present in the extracellular matrix are collagen and elastin, which are responsible for the mechanical properties of tissues such as their ability to resist tension, compression, extensibility, and warping.

Soybean paste is one of our traditional fermented foods made from soybeans, which are meat from the field, with high protein and fat content, and it serves as a seasoning food that adds taste and aroma to our diet based on cereals. It is a food with nutritional excellence that supplements essential amino acids, fatty acids, organic acids, minerals, vitamins, etc. that are prone to lack. Today, with the change of housing type and social structure, miso manufacturing is moved from each family to the factory, and food manufacturing technology has developed.

However, a cosmetic composition using natural products for maintaining and improving the function and beauty of the skin as described above is provided, but a cosmetic composition using miso having many physiological activities as a main component is not provided.

1. Korea Registered Patent No. 10-0501294-0000 2. Korea Registered Patent No. 10-1291187-0000

Therefore, the technical problem to be achieved by the present invention is to provide a cosmetic composition excellent in anti-wrinkle effect using miso as a main component.

In order to achieve the above object to be solved, the present invention

It provides a cosmetic composition characterized by including the miso powder, the situation mushroom powder and dietary fiber powder as an active ingredient.

The cosmetic composition according to the present invention is preferably contained in the miso powder 94 to 98% by weight, situation mushroom powder 1.0 to 3.0% by weight, dietary fiber powder 1.0 to 3.0% by weight.

The cosmetic composition according to the present invention may include the miso powder, the situation mushroom powder and the dietary fiber powder dissolved in a solvent in any one selected from the group consisting of water, 1-3 butylene glycol and mixed solvents thereof. .

In the cosmetic composition according to the present invention, it is preferable that the miso powder is made of unsalted miso.

The cosmetic composition according to the present invention may further contain 1.0 to 10.0% by weight of seaweed hot water extract, based on the total weight of the composition, wherein the seaweed is at least one selected from the group consisting of persimmon, kelp, seaweed, gompi and rhubarb. desirable.

In the cosmetic composition according to the present invention, the seaweed hot water extract may be prepared by enzymatic reaction by extracting seaweed using hot water and adding a culture medium in which a microorganism producing polysaccharide degrading enzyme is cultured.

Antioxidant effect, skin whitening effect, anti-inflammatory effect, anti-wrinkle effect, anti-atopic effect, miso powder, medicinal mushroom powder, dietary fiber powder and seaweed extract containing as an active ingredient cosmetic composition according to the present invention is a unique function of the skin It is competitive as a cosmetic that maintains and enhances beauty and beauty.

1 is a diagram showing the test results for cell viability for Human dermal fibroblast (HDF) cells of the cosmetic composition according to the present invention.
2 is a diagram showing the results of measuring the Elastase inhibitory activity for evaluating anti-wrinkle efficacy of the cosmetic composition according to the present invention.

Hereinafter, specific contents for carrying out the present invention will be described.

The present invention provides a cosmetic composition containing an excellent anti-wrinkle effect of miso powder, situation mushroom powder and dietary fiber powder as an active ingredient. The miso used in the present invention is a miso widely known in the art to which the present invention pertains. It can be used without limitation, and it is okay to use a thing containing a small amount of functional ingredients other than soybeans, and since it is used for the skin, it is preferable to use it with low salinity, and most preferably, miso with no salt at all Is to use

The situation mushroom used in the present invention is also referred to as woody mud mushroom, and in Donguibogam, it is recorded on the sap of water under the name Sangmok-i. The shade is 6-12cm in diameter and 2-10cm in thickness, and has various shapes such as semicircle shape, flat shape, round mountain shape, and horseshoe shape. On the surface, dark brown hairs are short and dense, and then disappear and peel off as they grow. There is a black brown ring groove and the back is split horizontally and vertically. The edges are vivid yellow, the underside is yellowish brown, and the flesh is yellowish brown. There are no sacks, and the spores are light yellowish brown and have a ball shape. It is a perennial perennial tree that grows on mulberry trees. It is known to have excellent anti-cancer effects. It is characterized by yellow or light yellow color when used for medicinal purposes, and has no taste and aroma.

In addition, the dietary fiber used in the present invention is a component known as fiber or cellulose contained in vegetables, fruits, seaweeds, etc. in food, and refers to a polymer carbohydrate that is not digested by human digestive enzymes and is discharged out of the body. Any dietary fiber widely used in the technical field can be used without particular limitation.

The above-described miso, oyster mushroom and dietary fiber are used as dry powders, but there are no particular restrictions on low tide methods such as natural drying, freeze drying and vacuum drying, and there is no particular limitation on the method of powdering and the size of powder particles.

In addition, the cosmetic composition according to the present invention is a miso powder, situation mushroom powder, dietary fiber powder composition ratio is not particularly limited, in order to prevent wrinkles as a result of various tests, the miso powder 94 to 98% by weight, situation mushroom powder 1.0 to 3.0 It is preferable to contain 1.0% by weight to 3.0% by weight of dietary fiber powder.

And in the present invention, when the miso powder, oyster mushroom powder and dietary fiber powder are difficult to be included in powder form, dissolve in a solvent in any one selected from the group consisting of water, 1-3 butylene glycol, and mixed solutions thereof. In this case, in this case, it is preferable to use the solvents after dissolving the powders in excess and dissolving as much as possible and then filtering and removing the insoluble ones.

In addition, the cosmetic composition according to the present invention may further contain 1.0 to 10.0% by weight of the seaweed hot water extract, based on the total weight of the composition.If the seaweed hot water extract is further included, wrinkles are caused by polysaccharides contained in the seaweed hot water extract. This is because the prevention effect is further enhanced.

Limiting the content range of the seaweed hot water extract to 1.0 to 10.0% by weight is that the minimum range and maximum range exerting a synergistic effect are set through various tests. This is because it decreases more.

In addition, the present invention is by hot water extraction using seaweed as a solvent, it is possible to avoid problems caused by the solvent. That is, in the case of a solvent other than water, there may be a problem such as removal of the solvent for safety reasons, but in the case of hot water extraction using water as a solvent, such a problem can be avoided. In addition, polysaccharides can be effectively extracted from seaweeds by hot water extraction.

The seaweed that can be used in the present invention is not particularly limited, but preferably, it is preferable to use any one or more selected from the group consisting of persimmon, kelp, seaweed, gompi, and antagonist. There are no specific restrictions, but the weight is based on the weight: Tashima: seaweed: Gompi: rhubarb = 50 to 80: 5 to 10: 5 to 10: 5 to 10: 5 to 10, most preferably weight based on emotion: Dashima : Seaweed: Gompi: Rhubarb = 75:5:5:5:10 to extract by mixing, it is possible to effectively produce a seaweed extract containing a variety of polyphenols by such a configuration.

The persimmon (甘苔) is a perennial seaweed with brown seaweed kelp tree seaweed, scientific name is Ecklonia cava , kelp is a seaweed with brown seaweed kelp tree kelp family, scientific name is Leminaria japonica , and seaweed is one year with brown seaweed In seawater, the scientific name is Undaria pinnatifida , the perennial seaweed with brown seaweed kelp tree seaweed, the scientific name is Ecklonia stolonifera , the rhubarb is the seaweed with the brown seaweed seaweed, and the scientific name is Eisenia bicyclis .

The method for extracting hot water used in the present invention is not particularly limited as long as it is well known in the technical field to which the present invention pertains. Preferably, 500 to 2000 parts by weight of water is added to 100 parts by weight of seaweed, and 70 to 95°C for 1 to 3 hours. To extract by heating, more preferably, 1000 parts by weight of water is added to 100 parts by weight of seaweed, followed by extraction by heating at 95°C for 3 hours.

The seaweed hot water extract as described above can be used directly as an active ingredient of the cosmetic composition according to the present invention without any other treatment, and more preferably, the hot water extract is enzymatically reacted with a polysaccharide degrading enzyme to be easily absorbed by the skin. It can be used by converting the polysaccharide to the form.

To explain this process in more detail, the seaweed hot water extract filtration step, the polysaccharide degrading enzyme culture step and the enzymatic reaction step can be divided into, and the seaweed hot water extract filtration step is the seaweed hot water extract prepared as described above. This is the process of removing seaweed by filtration.

In the filtration step, filtration can be used without particular limitation as long as it can remove seaweed, and is preferably filtered with a filtration membrane having pores of 0.2 to 1 μm, more preferably 0.45 μm. The filtrate not only removes algae, but also contains polysaccharides having a kDa of 1000 kDa or less to improve the efficiency of the enzymatic reaction.

The manufacturing step of the polysaccharide degrading enzyme culture solution consists of culturing a microorganism that produces a polysaccharide degrading enzyme, and then removing the cells, which will be described in detail as follows.

Here, the polysaccharide-degrading enzyme-producing microorganism is not limited as long as it can produce an enzyme capable of decomposing polysaccharide to produce polyphenols, for example, Pseudomonas sp . ), Thalassomonas sp. , Agarivorans albus ), Pseudoalteromonas sp . ), and Pseudoalteomonas carrageenovor , preferably Pseudomonas angulata , Thalassomonas piscicida ), Agarivorans albus ), Pseudoalteromonas citrea), and Pseudomonas Pseudomonas know Theo Kara Saginaw bore (Pseudoalteomonas carrageenovor ).

As such, various polyphenols can be produced by a combination of microorganisms capable of producing various polysaccharide degrading enzymes. That is, five microorganisms produce different polysaccharide degrading enzymes, and each enzyme can easily cut several parts of polysaccharide to produce various types of polyphenols.

The culture medium containing the polysaccharide-degrading enzyme can be prepared by culturing the polysaccharide-degrading enzyme-producing microorganism in the medium. At this time, it is possible to prevent the microbial contained in the seaweed extract by removing the cells consisting of microorganisms in the culture medium, it is possible to effectively enzymatic reaction. Removal of the cells may be achieved by adjusting the pH of the culture solution after completion of the culture to preferably 8 to 9, more preferably pH 8.5, and then centrifuging to take the supernatant. The pH can be adjusted with sodium hydroxide, etc., and centrifugation can be carried out at 4000 rpm for 30 minutes. In addition, the culture solution containing the polysaccharide degrading enzyme may be taken by adjusting the pH of the supernatant to preferably 6-7, more preferably 6.5. At this time, the pH can be adjusted with an acid such as 35% dilute hydrochloric acid. By adjusting the pH in this way, it is possible to maintain the activity of the enzyme remaining after cell separation.

Thereby, a polysaccharide degrading enzyme having a polysaccharide decomposition activity of 600 units/L or more can be obtained. At this time, the enzyme decomposition activity can be calculated using a method such as DNS method. For example, after mixing 500 µl of the polysaccharide-degrading enzyme culture with 1.0 ml of the polysaccharide-containing extract, reacting in a thermostat for 30 minutes (150 rpm, 25° C.), and centrifuging the reaction solution (14,000 g, 4° C., After 5 minutes), 2 ml of a DNS (3,5-dinitrosalicylic acid) solution was added to 500 μl of the centrifuged supernatant, followed by heating for 10 minutes and measuring absorbance at 540 nm. Reducing sugar produced is quantified using a standard calibration curve prepared using mannuronic acid, and the ability to produce reducing sugar is considered to be proportional to the activity of the polysaccharide degrading enzyme. One unit of enzyme can be defined as the amount of enzyme that produces 1 micromolar of reducing sugar per minute. By this method, the polysaccharide degrading enzyme can be calculated to have a polysaccharide decomposition activity of 600 units/L or more.

The enzymatic reaction step is to perform an enzymatic reaction by mixing the seaweed hot water extract and a polysaccharide degrading enzyme culture solution, but is not limited thereto. For example, in detail, for 10 to 30 hours at 30 to 40°C, preferably It may be carried out for 20 to 30 hours, more preferably it is carried out at 35 ℃ for 30 hours.

In this range, an effective enzymatic reaction may occur, and the polyphenol content may increase. Below this range, there is a concern that the increase in the polyphenol content may not be large depending on the elapsed time of the enzymatic reaction, and when the range is exceeded, the polyphenol content may decrease rather.

In addition, the algae extract and the culture solution containing the polysaccharide degrading enzyme can be enzymatically reacted by mixing at a certain ratio, and preferably mixed at a ratio of 1:1 by weight.

The seaweed extract of the present invention contains polyphenol, and the polyphenol may be 30% by weight or more based on the total weight of the seaweed extract.

And although not essential, the purification step may be additionally performed for preparing the seaweed extract of the present invention. The purification step may be a step of taking the filtrate after treating the enzyme reaction solution at 90 to 99°C for 20 to 40 minutes, more preferably at 95°C for 30 minutes after completion of the enzyme reaction. The enzyme and protein contained in the enzyme reaction solution are entangled and condensed by the heat treatment. The condensed enzyme and protein can be removed by filtration using a pore size 0.1 micron filtration membrane. Enzymes and the like are removed from the filtrate taken by such filtration to produce a seaweed extract containing purified polyphenols.

The cosmetic composition in the present invention as described above may include components commonly used in cosmetic compositions, for example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers. Includes.

Such a cosmetic composition may be prepared in any formulation conventionally manufactured in the art. In one embodiment, it may be any one formulation selected from the group consisting of high-viscosity emulsifier formulations, low-viscosity emulsifier formulations and solubilizing formulations, but is not limited thereto. Depending on the formulation to be prepared, it may include a cosmetic composition compounding ingredients commonly used in the art. Examples include solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, packs, massage creams and sprays. However, it is not limited thereto. More specifically, flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, sun lotion, sun cream, makeup base, BB cream, cleansing cream, cleansing foam, cleansing water, pack, stick products, balm ( Balm) type products, sprays or powders.

Depending on the formulation to be prepared, additionally water components, oil components, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, preservatives, fragrances, etc. can be selected and added in combination.

The cosmetic composition according to the present invention may include miso powder, yellow mushroom powder and dietary fiber powder in an amount of 0.001 to 30% by weight based on the total weight. When the powders are less than 0.001% by weight based on the total composition weight, the targeted effect is not exhibited, and when it exceeds 30% by weight, the degree of increase in the effect of increasing the content is negligible, and there is a problem in the stability of the formulation, and economical It is not.

Hereinafter, the present invention will be described in detail by the following examples and experimental examples. However, the following examples and experimental examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following examples and experimental examples.

Example One: Active ingredient Manufacturing method

After dissolving for 24 hours after adding 960 g of unsalted soybean paste powder, 20 g of Phellinus linteus powder and 20 g of dietary fiber powder to 1 l of mixed solvent mixed with purified water and 1-3 butylene glycol at a ratio of 1 to 1, the powder was no longer dissolved. It was filtered to prepare an active ingredient that can be used in the cosmetic composition according to the present invention.

Example 2. Anti wrinkle Efficacy evaluation

2-1. Cell culture

Human dermal fibroblast (HDF) cells were purchased from a Korean cell line bank and cultured in a 37°C, 5% CO ₂ incubator using Dulbecco's modified Eagle's medium medium containing 1% antibiotic and 10% fetal bovine serum. Passage culture was performed once a day.

2-2. Cytotoxicity measurement

◎ Experiment method

HDF cells were added to a 96 well plate at 1.0 × 10 ⁴ cells/ml using DMEM medium with 10% FBS added, and after 18 hours of incubation, the active ingredients were diluted by concentration and treated for 24 hours. Then, 50 µl of the MTT solution was added and reacted for 4 hours. After the culture medium was completely removed and 200 µl of dimethylsulfoxide was added to completely dissolve the precipitate, absorbance at 540 nm was measured using a microplate reader. The average absorbance value for each sample group was obtained, and the cell growth rate was evaluated by comparing with the absorbance value of the control group.

◎ Experiment result

The average absorbance value for each sample group was obtained, and the cell growth rate calculated by comparing with the absorbance value of the control group is shown in FIG. 1. Referring to FIG. 2, it shows 100% survival rate in the active ingredient at a concentration of 125 μg/ml, showing cytotoxicity. It was confirmed that it does not appear.

Example 3: Preparation of seaweed extract

After crushing hot air dried kelp, kelp, seaweed, gompi and rhubarb to a size of 20 mesh with a moisture content of less than 5%, the persimmon is weighted by weight: Tashima: seaweed: gompi: rhubarb = 75:5:5:5:10 After mixing persimmon, kelp, seaweed, gompi, and rhubarb, 1000 parts by weight of purified water was added to 100 parts by weight of the algae, and heated to 95°C for 3 hours to extract hot water, filtered through a filtration membrane having a pore size of 0.45 μm, and having a molecular weight of 1000 kDa or less Seaweed hot water extract containing a polysaccharide having a was prepared.

Example 4: Anti wrinkle Efficacy evaluation-Synergy effect evaluation with seaweed extract

One) Elastase Inhibition activity measurement

● Experimental Method

Each test solution is prepared to have a constant concentration. After adding 40 μL of proporcine pancreas elastase (2.5 U/mL) solution dissolved in 50 mM Tris-HCl buffer (pH 8.6) to 40 μl of each test solution, N-succinyl- dissolved in 50 mM Tris-HCl buffer (pH 8.6) as a substrate. 80 L of (L-Ala)3-p-nitroanilide (0.5 mg/ml) was added and mixed. After reacting at 37°C for 30 minutes, absorbance was measured at 410 nm. Elastase inhibitory activity was expressed as the rate of decrease in absorbance of the sample solution with and without addition.

Inhibition rate (%) = 1-(absorbance of the sample-added group/absorbance of the sample-free group) x 100

● Experimental results

To confirm whether the effect can be increased by mixing the seaweed extract with the miso tea of Example 1, the concentration of each miso tea of Example 1 and the concentration of 250 μl/ml of the seaweed extract were measured to measure elastase inhibitory activity. The results were attached to FIG. 12, and seeing FIG. 2, there was no effect as a result of measuring the elastase inhibitory activity against miso tea of Example 1, but by mixing 250 μl/ml of seaweed extract for each concentration of miso tea It was confirmed that there was a synergistic effect.

Claims (7)

Cosmetic composition characterized by including miso powder, oyster mushroom powder and dietary fiber powder as active ingredients.
The cosmetic composition according to claim 1, wherein the miso powder is contained in an amount of 94 to 98% by weight, oyster mushroom powder in an amount of 1.0 to 3.0% by weight, and dietary fiber powder in an amount of 1.0 to 3.0% by weight.
The cosmetic composition according to claim 1 or 2, wherein the miso powder is made of unsalted miso.
The method of claim 1 or claim 2, wherein the miso powder, matsutake mushroom powder and dietary fiber powder is dissolved in a solvent in any one selected from the group consisting of water, 1-3 butylene glycol and mixed solvents thereof. Cosmetic composition characterized in that it is included.
The method of claim 4,
Cosmetic composition characterized in that it further comprises 1.0 to 10.0% by weight based on the total weight of the seaweed hot water extract.
The cosmetic composition according to claim 5, wherein the seaweed is at least one selected from the group consisting of persimmon, kelp, seaweed, gompi and rhubarb.
The method of claim 4 or 5, wherein the seaweed hot water extract is produced by enzymatically reacting by extracting a culture medium in which a microorganism producing polysaccharide degrading enzyme is added to the extract after extracting the seaweed using hot water. Cosmetic composition.

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