KR20200075813A - 개선된 철-황 클러스터 전달을 갖는 세포 공장 - Google Patents

개선된 철-황 클러스터 전달을 갖는 세포 공장 Download PDF

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KR20200075813A
KR20200075813A KR1020207002518A KR20207002518A KR20200075813A KR 20200075813 A KR20200075813 A KR 20200075813A KR 1020207002518 A KR1020207002518 A KR 1020207002518A KR 20207002518 A KR20207002518 A KR 20207002518A KR 20200075813 A KR20200075813 A KR 20200075813A
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한스 예스퍼 히니
애네 피으 벨리
닐스 밀링-피터슨
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Abstract

본 발명은 돌연변이 철 황 클러스터 조절자 (IscR)를 코딩하는 변형된 유전자뿐만 아니라 비오틴, 리포산 또는 티아민의 생합성을 증가시키는 폴리펩티드를 코딩하는 하나 이상의 전이 유전자를 특징으로 하는, 개선된 철-황 클러스터 전달이 가능한 유전자 변형 박테리아 세포를 제공한다. 본 발명은 본 발명의 유전자 변형 박테리아를 사용하여 비오틴, 리포산 또는 티아민을 생산하는 방법뿐만 아니라; 비오틴, 리포산 또는 티아민 생산을 위한 유전자 변형 박테리아 세포의 사용을 제공한다.

Description

개선된 철-황 클러스터 전달을 갖는 세포 공장
본 발명은 돌연변이 철 황 클러스터 조절자 (IscR)를 코딩하는 변형된 유전자뿐만 아니라 비오틴, 리포산 또는 티아민의 생합성을 증가시키는 폴리펩티드를 코딩하는 하나 이상의 전이 유전자를 특징으로 하는, 개선된 철-황 클러스터 전달이 가능한 유전자 변형 박테리아 세포에 관한 것이다. 본 발명은 또한 본 발명의 유전자 변형 박테리아를 사용하여 비오틴, 리포산 또는 티아민을 생산하는 방법뿐만 아니라; 비오틴, 리포산 또는 티아민 생산을 위한 유전자 변형 박테리아 세포의 사용에 관한 것이다.
비오틴 (비타민 B7 또는 비타민 H라고도 알려짐), 및 티아민 (비타민 B2이라고도 알려짐)은 사람에게 필수적인 식이 비타민인데, 이는 다른 후생동물들과 마찬가지로, 그들은 비오틴 또는 티아민을 생산할 수 없기 때문이다. 리포산 (LA: Lipoic acid)은 황-함유, 비타민-유사 항산화제이고, 박테리아, 식물 및 동물에서 소량으로 합성된다. 세가지 모두 식이 보충제로 널리 사용된다. 이들 비타민 또는 비타민-유사 화합물들의 생산은 현재 화학적 합성에 의존하는데, 이는 비용이 많이 든다. 이들의 제조를 위한 생합성 방법은 현재 및 미래 요구를 충족시키기 위한 대안적이고, 보다 비용효율적인 방법을 제공할 것이다.
비오틴은 모든 생물 형태에 존재하는 아세틸-CoA 카복실라제 (ACC: acetyl-CoA carboxylase)와 같은 특정 카르복실화 반응을 촉매시키는 효소의 필수적인 보조인자이고, 지방산 생합성의 중요한 구성 요소인 말로닐-CoA를 생산한다. 사실상, 비오틴은 지방산 생합성 경로와 관련된 선형 경로에 의해 합성된다. 대장균 (E. coli: Escherichia coli)에서 비오틴 생합성의 초기 기질은 지방산 합성의 개시 대사 산물이기도 한 말로닐-ACP이다. 지방산 사이클을 들어가기 전에, 말로닐-ACP는 SAM (S-아데노실메티오닌)-의존적 메틸트랜스퍼라제, BioC에 의해 마스킹되어, 말로닐-ACP 메틸 에스테르를 생성한다. 이어서, 2회의 지방산 사슬 신장은 피멜로일-에스테르-ACP 분자를 생성한다. 전용 에스테라제, BioH에 의한 피멜로일-에스테르-ACP의 O-메틸기의 가수분해는 이들 분자를 지방산 신장 사이클을 빠져나가게 한다. 이어서, 중간체(intermediate), 피멜로일-에스테르-ACP는 비오틴-특이적 경로를 통해 비오틴으로 전환된다 (도 1A). 이 경로에서, BioF는 피멜로일-ACP와 알라닌의 PLP-의존적 탈탄산 알돌 축합(decarboxylative aldol condensation)을 촉매하여 KAPA (8-아미노-7-옥소노나노에이트)를 생산한다. BioA (및 BioK)는 KATA의 PLP-의존적 아미노기 전이반응을 촉매하여 DAPA (7,8-디아미노펠라르고네이트)를 생산하고, 여기에서 공여자는 SAM이고; 부산물은 S-아데노실 옥소메티오닌이다. BioD는 ATP-구동 카르복실화 및 DAPA의 고리 폐쇄를 촉매하여 데스티오비오틴 (DTB: desthiobiotin)에서 티오펜(thiophane) 고리를 형성한다. 비오틴 합성 경로의 최종 단계는 BioB (비오틴 신타아제)에 의해 2개의 탄화수소 사이에 황 다리 결합(sulfur bridge)의 도입과 관련되어 있으므로, 비오틴을 생산하기 위한 알려진 가장 복잡한 반응 중 하나이다. BioB는 이합체로 발견되는, S-아데노실-L-메티오닌 (SAM 또는 AdoMet) 라디칼 효소이고, 이의 활성 부위에 2개의 철-황 클러스터: [2Fe-2S]　2+　및 [4Fe-4S]　2+를 포함한다. DTB에서 티오펜 고리를 생성하는데 필요한 황 원자는 BioB에서 [2Fe-2S]　2+　클러스터로부터 조달된 것으로 여겨진다. 결과적으로, DTB 합성에 소비되는 BioB 이합체에서 철-황 클러스터는 촉매반응의 각 라운드 후에 재생산되는 것으로 생각된다.
리포산 (LA: Lipoic acid)는 활성 산소종의 강력한 스캐빈저(scavenger)일 뿐만 아니라, 이에 따라 중요한 항-산화제이고, 또한 α-케토산 탈수소효소에 대한 보조인자이다. LA는 지방산 대사과정에서 중간체로부터 새롭게 합성된다 (도 2). E. coli에서 LA 합성에 참여하는 3개의 효소는 LplA (리포산-단백질 리가아제), LipB (옥탄오일 단백질 ACP 운반 단백질: 단백질 트랜스퍼라제), 및 LipA (리포산 신타아제)이다. lplA 유전자에 의해 코딩되는 LplA는 ATP-의존적인 방식으로 타겟 효소의 E2 서브유닛의 비리포일화된-아포-리포일 도메인(unlipoylated-apo-lipoyl domain)에 외인성 옥탄산의 컨쥬게이션을 촉매할 수 있다. lipB 유전자에 의해 코딩되는 LipB는 ACP로부터의 옥타닐 잔기의 타겟 효소의 E2 서브유닛의 아포-리포일 도메인으로의 이동을 촉매할 수 있다. AceF 유전자는 피루브산 탈수소효소의 E2 서브유닛의 리포일 도메인을 코딩한다. lipA 유전자에 의해 코딩되는 LipA는 2개의 C-S 결합의 형성을 담당한다. LipA-유도 반응은 이의 기능을 수행하기 위해 철-황 클러스터 (4Fe-4S) 및 SAM (metK 유전자에 의해 생성됨)을 필요로 한다. 리포산은 주로 다수의 다중-효소 복합체에서 단백질-결합된 리포아미드(lipoamide) 모이어티로서 세포에서 발견된다.
티아민 생합성은 박테리아, 일부 원생동물, 식물, 및 진균에서 특성화되었다. 티아민의 티아졸 및 피리미딘 모이어티는 별도로 합성된다 (도 3). 피리미딘 모이어티, 4-아미노-5-하이드록시메틸-2-메틸피리미딘 포스페이트 (HMP-P)는 드 노보(de novo) 퓨린 생합성 경로에서 중간체인 5-아미노이미다졸 리보타이드 (AIR: aminoimidazole ribotide)로부터 유래된다. 그람-음성 박테리아에서, AIR의 HMP-P로의 전환은 서브유닛 당 1개의 [4Fe-4S] 클러스터에 결합하는 thiC 유전자 산물인 HMP-P 신타제에 의한 라디칼 S-아데노실-L-메티오닌 (SAM)-의존적 반응에서 촉매된다.
이어서, HMP-P는 티아졸 유닛과의 커플링 전에 ThiD 키나아제에 의해 HMP-PP로 인산화된다. 티아졸 모이어티, 5-(2-하이드록시메틸)-4-메틸티아졸 포스페이트 (HET-P)는 L-티로신 및 1-데옥시-D-자일룰로스 포스페이트 (DXP) 및 시스테인으로부터 유래되고; 여기어세 황 원자는 L-시스테인으로부터 유래되는 것으로 예상된다. thiH 유전자에 의해 코딩되는 티로신 리아제(Tyrosine lyase)는 서브유닛 당 1개의 [4Fe-4S]에 결합하고, 티로신의 2-이미노아세테이트 및 4-크레솔로의 라디칼-매개 절단을 촉매작용한다. 티아졸 모이어티의 합성은 적어도 5개의 유전자 thiF, this, thiG, thiH 및 thiI의 발현을 필요로 한다.
이후, 피리미딘 및 티아졸 모이어티는 thiE에 의해 코딩되는 티아민-포스페이트 신타아제 (EC 2.5.1.3)의 작용에 의해 TMP를 형성하도록 조합된다. 따라서, TMP는 모든 공지된 티아민 생합성 경로의 첫번째 산물이다. 대장균 및 다른 장내 세균에서, TMP는 ATP의 존재 하에 thiL에 의해 코딩되는 티아민-포스페이트 키나아제 (EC 2.7.4.16)에 의해 보조인자 TPP로 인산화될 수 있다. 티아민 모노-포스페이트 포스파타제 (E.C 3.1.3.-)를 발현하는 전이 유전자를 포함하는 박테리아 균주들은 TMP를 티아민으로 전환시킬 수 있고, 이로써 티아민 생산을 증가시킬 수 있다.
박테리아-기반의 세포 공장의 사용은 비오틴, 리포산 및 티아민의 생합성 생산을 위한 잠재적 경로이다. 바이오-제품의 생산을 위한 세포 공장으로서 재조합 대장균의 이점은 다음과 같은 사실로 인해 널리 인식된다: (i) 이는 글루코스-염 배지에서 배양될 경우 및 최적의 환경 조건 하에서 약 20분의 배가 시간(doubling time)을 가진 비할데 없는 빠른 성장 속도를 갖는다, (ii) 높은 세포 밀도를 쉽게 달성한다; 여기에서 대장균 액체 배양물의 이론적 밀도 한계는 약 200 g 건조 세포 중량/l 또는 대략 1 x 1013　생존 가능한(viable) 박테리아/mL로 추정된다. 또한, 대장균은 이종 단백질의 발현에 다루기 쉬운(amenable) 유기체일 뿐만 아니라; 대장균의 유전자 변형을 위한 많은 분자 도구 및 사용 가능한 프로토콜이 있다; 이들 모두는 원하는 바이오-제품의 높은-수준의 생산을 수득하기 위해 필수적일 수 있다.
대장균에서, 비오틴 오페론 구조는 반대 가닥(bioO 유전자좌) 상의 중복 프로모터의 조절 하에 bioA 및 bioBFCD로 나눠지는데, bioH는 대장균 염색체의 다른 곳에 위치한다. 비오틴 오페론의 발현은 비오틴-결합 억제자 (BirA: biotin-bound repressor)에 의해 하향-조절되고; 비오틴-결합 억제자는 비오틴 오페론에서 오퍼레이터(operator)에 결합한다. BirA은 또한 비오틴을 세포의 카르복실라제로 전달하는 비오틴 리가아제로서 기능을 한다. 비오틴 리가아제로부터 전사 억제자로의 BirA의 기능 전환은 각각의 세포 내 비오틴 및 아포-카복실라제 풀에 의해 조절된다. 대장균에서 비오틴 오페론 (bioA 및 bioBFCD)의 과-발현은 성장을 저해하는 것으로 보고되었다 (Ifuku, 0. et al., 1995). 이 저해의 원인은 알려져 있지 않았고, 이는 비오틴 합성을 증가시키는 장애물을 형성한다.
일반적으로, 박테리아-기반의 세포 공장 (예를 들어, 대장균)에서 비오틴, 리포산 및 티아민의 생산을 용이하게 하기 위해 이러한 복잡한 생합성 경로들의 병목 현상을 규명할 필요가 있고, 박테리아-기반의 세포 공장은 이들 각각의 경로 효소들의 증가된 수준을 성장 및 생산하는 이들의 능력을 제한할수 있는 다양한 원인을 극복하도록 맞춤-제작된다.
발명의 요약
일 양상에 따르면, 본 발명은 비오틴, 리포산 또는 티아민 중 어느 하나의 증가된 생산을 위해 유전자 변형 박테리아를 제공하며; 상기 박테리아는:
ㆍ돌연변이 IscR 폴리펩티드를 코딩하는 유전자로 변형 내인성 iscR 유전자로서, 상기 돌연변이 IscR 폴리펩티드의 아미노산 서열은 서열번호 2, 4, 6, 8, 10, 12 및 14와 80% 서열 상동성을 갖는 것이고, 상기 아미노산 서열은 다음으로 이루어진 군으로부터 선택된 적어도 하나의 아미노산 치환을 갖는 것인:
o L15X, C92X, C98X, C104X, and H 107X; 상기 X는 서열번호 2, 4, 6, 8, 10, 12 및 14에 상응하는 아미노산 잔기 이외의 임의의 아미노산인 것인 유전자, 및
ㆍ 이들 중 선택된 폴리펩티드를 코딩하는 적어도 하나의 전이유전자:
o 증가된 비오틴 생산을 위한 비오틴 신타아제 (EC 2.8.1.6) 활성을 갖는 폴리펩티드,
o 증가된 리포산 생산을 위한 리포산 신타아제 (EC 2.8.1.8) 활성을 갖는 폴리펩티드,
o 증가된 티아민 생산을 위한 HMP-P 신타아제 (EC 4.1.99.17) 활성을 갖는 폴리펩티드, 및
o 증가된 티아민 생산을 위한 티로신 리아제 (EC 4.1.99.19) 활성을 갖는 폴리펩티드.
바람직하게, 상기 돌연변이 IscR 폴리펩티드 내 적어도 하나의 아미노산 치환은 다음으로 이루어진 군으로부터 선택된다:
o L15X, 상기 X는 F, Y, M 및 W 중 어느 하나임;
o C92X, 상기 X는 Y, A, V, I, G, L, M, F 및 W 중 어느 하나임;
o C98X, 상기 X는 A, V, I, G, L, F 및 W 중 어느 하나임;
o C104X, 상기 X는 A V, I, G, L, F 및 W 중 어느 하나임; 및
o H 107X; 상기 X는 A, Y, M, F, W, V, I, G, 및 L 중 어느 하나임.
비오틴 신타아제 (EC 2.8.1.6) 활성을 갖는 폴리펩티드를 코딩하는 하나의 전이 유전자를 포함하는 비오틴의 증가된 생산을 위한 본 발명에 따른 유전자 변형 박테리아는 다음으로 이루어진 군으로부터 선택된 하나 이상의 폴리펩티드를 코딩하는 추가적인 전이 유전자를 더 포함할 수 있다:
o SAM (S-아데노실메티오닌)-의존적 메틸트랜스퍼라제 (BioC; EC 2.1.1.197) 활성을 갖는 폴리펩티드;
o 7-케토-8-아미노펠라르곤산 (KAPA) 신타아제 (BioF; EC 2.3.1.47) 활성을 갖는 폴리펩티드;
o 7,8-디아미노펠라르곤산 (DAPA) 신타아제 (BioA; EC:2.6.1.62) 또는 L-리신:8-아미노-7-옥소노나노에이트 아미노트랜스퍼라제(BioK; EC:2.6.1.105) 활성을 갖는 폴리펩티드;
o 데티오비오틴 (dethiobiotin, DTB) 신타아제 (BioD; EC 6.3.3.3) 활성을 갖는 폴리펩티드, 및
o 피멜로일-[아실-운반 단백질] 메틸 에스테르 에스테라제 (BioH; EC 3.1.1.85)를 갖는 폴리펩티드 또는 6-카복시헥사노에이트-CoA 리가제 (BioW; EC 6.2.1.14) 활성을 갖는 폴리펩티드.
바람직하게, 비오틴 신타아제 (EC 2.8.1.6) 활성을 갖는 폴리펩티드를 코딩하는 하나의 전이 유전자를 포함하는 비오틴의 증가된 생산을 위한 본 발명에 따른 유전자 변형 박테리아는 SAM (S-아데노실메티오닌)-의존적 메틸트랜스퍼라제 (BioC; EC 2.1.1.197) 활성; 7-케토-8-아미노펠라르곤산 (KAPA) 신타아제 (BioF; EC 2.3.1.47) 활성; 및 7,8-디아미노펠라르곤산 (DAPA) 신타아제 (BioA; EC:2.6.1.62) 활성을 갖는 폴리펩티드를 코딩하는 추가적인 전이 유전자를 더 포함할 수 있다.
리포산 신타아제 (EC 2.8.1.8) 활성을 갖는 폴리펩티드를 코딩하는 하나의 전이 유전자를 포함하는 리포산의 증가된 생산을 위한 본 발명에 따른 유전자 변형 박테리아는 다음으로 이루어진 군으로부터 선택된 하나 이상의 폴리펩티드를 코딩하는 추가적인 전이 유전자를 더 포함할 수 있다:
o 옥타노일트랜스퍼라제 (EC 2.3.1.181) 활성을 갖는 폴리펩티드, 및
o 피루브산 탈수소효소 (EC 2.3.1.12)의 디하이드로리포일라이신-잔기 아세틸트랜스퍼라제 성분을 포함하는 폴리펩티드, 및
o 리포에이트-단백질 리가아제 A (EC:6.3.1.20) 활성을 갖는 폴리펩티드.
HMP-P 신타아제 (EC 4.1.99.17) 활성을 갖는 ThiC 폴리펩티드를 코딩하는 하나의 전이 유전자, 및/또는 티로신 리아제(EC 4.1.99.19) 활성을 갖는 ThiH 폴리펩티드를 코딩하는 하나의 전이 유전자를 포함하는 티아민의 증가된 생산을 위한 본 발명에 따른 유전자 변형 박테리아는 다음으로 이루어진 군으로부터 선택된 하나 이상의 폴리펩티드를 코딩하는 추가적인 전이 유전자를 더 포함할 수 있다:
o ThiS 아데닐트랜스퍼라제 (EC 2.7.7.73) 활성을 갖는 ThiF 폴리펩티드;
o 티아민 포스페이트 신타아제 (EC 2.5.1.3) 활성을 갖는 ThiE 폴리펩티드;
o 티아졸 신타아제 (E.C.2.8.1.10) 활성을 갖는 ThiG 폴리펩티드;
o 포스포하이드록시메틸피리미딘 키나아제 (EC 2.7.4.7) 활성을 갖는 ThiD 폴리펩티드;
o 황-운반 단백질 활성을 갖는 ThiS 폴리펩티드;
o 모노-포스페이트 포스파타제 (E.C. 3.1.3.-) 활성을 갖는 폴리펩티드; 및
o 글리신 옥시다아제 (EC 1.4.3.19) 활성을 갖는 ThiO 폴리펩티드; 및 선택적으로는, 하이드록시에틸티아졸 키나아제 (2.7.1.50) 활성을 갖는 ThiM 폴리펩티드를 코딩하는 추가적인 전이 유전자.
바람직하게, 상기 유전자 변형 박테리아는 다음의 폴리펩티드를 코딩하는 전이 유전자를 포함한다: ThiC (thiC 유전자에 의해 코딩됨); ThiD (thiD 유전자에 의해 코딩됨), ThiE (thiE 유전자에 의해 코딩됨), ThiF (thiF 유전자에 의해 코딩됨), sulfur-carrier protein (thiS 유전자에 의해 코딩됨), ThiG (thiG 유전자에 의해 코딩됨), TMP phophatase (TMP 포스파타제 유전자에 의해 코딩됨); 및 ThiH (thiH 유전자에 의해 코딩됨) 또는 ThiO (thiO 유전자에 의해 코딩됨). 구현예에 따르면, 상기 세포는 효소 ThiM (ThiM 유전자에 의해 코딩된)을 코딩하는 전이 유전자를 더 포함할 수 있다.
바람직하게 본 발명의 유전자 변형 박테리아에서 상기 적어도 하나의 전이 유전자 및 상기 하나 이상의 추가적인 전이 유전자는 항시성 프로모터(constitutive promoter)에 작동 가능하게 연결된 것이다 (상기 프로모터는 전이 유전자를 포함한 오페론에 작동 가능하게 연결된 것일 수 있음).
본 발명의 유전자 변형 박테리아는 바람직하게 에셔리키아 (Escherichia), 바실러스 (Bacillus), 브레비박테리움 (Brevibacterium), 버크홀데리아 (Burkholderia), 캄필로박터 (Campylobacter), 코리네박테리움 (Corynebacterium), 슈도모나스 (Pseudomonas), 셀라티아 (Serratia), 락토바실러스 (Lactobacillus), 락토코커스 (Lactocooccus), 아시네토박터 (Acinetobacter), 슈도모나스 (Pseudomonas), 및 아세토박터 (Acetobacter)로 이루어진 군으로부터 선택된 속의 종, 보다 바람직하게 에셔리키아 또는 코리네박테리움의 종은, 예를 들어, 에셔리키아 콜라이 (Escherichia coli) 또는 코리네박테리움 글루타미쿰 (Corynebacterium glutamicum)이다.
제2 구현예에 따르면, 본 발명은 비오틴을 생산하는 방법으로서:
o 본 발명에 따른 비오틴 신타아제 (EC 2.8.1.6) 활성을 갖는 폴리펩티드를 코딩하는 전이 유전자를 포함하는 유전자 변형 박테리아를 배양물을 생산하기 위한 증식 배지 내로 도입하는 단계;
o 상기 배양물을 배양하는 단계; 및
o 상기 배양에 의해 생산된 비오틴을 회수하고, 선택적으로 회수된 비오틴을 정제하는 단계를 포함하는 방법을 제공한다.
제3 구현예에 따르면, 본 발명은 리포산을 생산하는 방법으로서:
o 본 발명에 따른 리포산 신타아제 (EC 2.8.1.6) 활성을 갖는 폴리펩티드를 코딩하는 전이 유전자를 포함하는 유전자 변형 박테리아를 배양물을 생산하기 위한 증식 배지 내로 도입하는 단계;
o 상기 배양물을 배양하는 단계; 및
o 상기 배양에 의해 생산된 리포산을 회수하고, 선택적으로 회수된 리포산을 정제하는 단계를 포함하는 방법을 제공한다.
제4 구현예에 따르면, 본 발명은 티아민을 생산하는 방법으로서:
o 본 발명에 따른 HMP-P 신타아제 (EC 4.1.99.17) 활성을 갖는 폴리펩티드를 코딩하는 전이 유전자, 및/또는 티로신 리아제 (EC 4.1.99.19) 활성을 갖는 폴리펩티드를 코딩하는 전이 유전자를 포함하는 유전자 변형 박테리아를 배양물을 생산하기 위한 증식 배지 내로 도입하는 단계;
o 상기 배양물을 배양하는 단계; 및
o 상기 배양에 의해 생산된 티아민을 회수하고, 선택적으로 회수된 티아민을 정제하는 단계를 포함하는 방법을 제공한다.
바람직하게 비오틴, 리포산 및 티아민 중 어느 하나를 생산하는 방법에서 사용된 증식 배지는 배지는 글루코스, 말토스, 갈락토스, 프럭토스, 수크로스, 아라비노스, 자일로스, 라피노스, 만노스, 및 락토스, 또는 이들의 임의의 조합으로부터 선택된 탄소원을 포함한다.
제4 구현예에 따르면, 본 발명은 비오틴 신타아제를 코딩하는 전이 유전자를 발현하는 박테리아 세포에서 비오틴 생산을 증가시키기 위해 돌연변이 iscR 폴리펩티드를 코딩하는 유전적으로 변형된 유전자의 용도를 제공한다. 상기 돌연변이 iscR 폴리펩티드는 서열번호 2, 4, 6, 8, 10, 12 및 14와 적어도 80% 서열 상동성을 갖는 것이고; 상기 아미노산 서열은 L15X, Cys92X, Cys98X, Cysl04X, 및 Hisl07X로 이루어진 군으로부터 선택된 적어도 하나의 아미노산 치환을 갖는 것이고, 상기 X는 서열번호 2, 4, 6, 8, 10, 12 및 14에 상응하는 아미노산 잔기 이외의 임의의 아미노산이다.
제4 구현예에 따르면, 본 발명은 박테리아에서 비오틴, 리포산 또는 티아민 중 어느 하나의 생산을 증가시키기 위한 돌연변이 iscR 폴리펩티드를 코딩하는 유전적으로 변형된 유전자의 용도를 제공한다. 상기 박테리아는 이들으로부터 선택된 폴리펩티드를 코딩하는 적어도 하나의 전이 유전자를 포함하고 발현한다:
ㆍ비오틴 신타아제 (EC 2.8.1.6) 활성을 갖는 폴리펩티드,
ㆍ리포산 신타아제 (EC 2.8.1.8) 활성을 갖느 폴리펩티드,
ㆍHMP-P 신타아제 (EC 4.1.99.17) 활성을 갖는 폴리펩티드, 및 티로신 리아제 (EC 4.1.99.19) 활성을 갖는 폴리펩티드.
상기 유전적으로 변형된 유전자는 돌연변이 IscR 폴리펩티드를 코딩하는 내인성 iscR 유전자이고, 상기 돌연변이 IscR 폴리펩티드의 아미노산 서열은 서열번호 2, 4, 6, 8, 10, 12 및 14와 적어도 80% 아미노산 서열 상동성을 갖는 것이고,
상기 아미노산 서열은: L15X, C92X, C98X, C104X, 및 H107X로 이루어진 군으로부터 선택된 적어도 하나의 아미산 치환을 갖는 것이고; 상기 X는 서열번호 2, 4, 6, 8, 10, 12 및 14에 상응하는 아미노산 잔기 이외의 임의의 아미노산이다.
제5 구현예에 따르면, 본 발명은 비오틴, 리포산 또는 티아민의 증가된 생산을 위한 본 발명에 따른 유전자 변형 박테리아의 용도를 제공한다.
제6 구현예에 따르면, 본 발명은 비오틴, 리포산 또는 티아민 중 어느 하나의 증가된 생산을 위한 본 발명에 따른 유전자 변형 박테리아를 제공한다. 상기 박테리아는 전자 공여자 NADPH로부터 SAM-라디칼 이온-황 클러스터 효소의 [4Fe-4S]2+　클러스터로의 증가된 전자 이동을 매개할 수 있는 폴리펩티드; 예를 들어, 플라보독신/페레독신 환원 효소 및 플라보독시 환원 시스템 또는 피루브산-플라보독신/페레독신 산화 환원 효소 시스템을 코딩하는 하나 이상의 유전자를 더 포함한다.
도 1 A) 박테리아의 비오틴 경로 및 비오틴의 합성을 일으키는 각각의 효소적 단계의 중간체를 나타내는 그림 (cartoon). SAM: S-아데노실-L-메티오닌, SAH: S-아데노실-L-호모시스테임, CoA: 코엔자임 A, ACP: 아실기 운반 단백질, KAPA: 7-케토-8-아미노펠라르곤산, AMTOD: S-아데노실-2-옥소-4-티오메틸부틸레이트, DAPA: 7,8-디아미노펠라르곤산, DTB: 데스티오비오틴, 5'DOA: 5'-데옥시아데노신. B) isc-오페론 구조 및 IscR의 조절 메커니즘뿐만 아니라 Fe-S-클러스터 형성에서의 역할을 나타내는 그림. 상기 isc 오페론은 다음의 유전자들의 발현을 조절하는 IscR을 코딩하는 iscR 유전자를 포함한다: iscS (시스테인 디설퍼라제 (cysteine desulphurase)), iscU (스캐폴드), iscA (A-타입 단백질), HscB (Dnaj-유사 코-샤페론), HscA (DnaK-유사 샤페론), 및 fdx (페레독신). IscR은 또한 hyaA, ydiU, erpA, 및 sufA 유전자를 포함하는 > 40 유전자를 조절한다.
도 2 박테리아의 리포산 경로 및 리포일화된 리포일 도메인 (리포산 합성)을 일으키는 각각의 효소적 단계의 중간체를 나타내는 그림. 상기 경로에서의 주요한 효소는 LipA (리포산 신타아제) 및 LipB (옥타노일 단백질 ACP 운반 단백질: 단백질 트랜스퍼라제)를 포함하고, 기질은 SAM: S-아데노실-L-메티오닌. LipA는 리포에이트-단백질 리가아제 A; EC: 6.3.1.20이다.
도 3 박테리아의 티아민 경로 및 티아민 (THI); 티아민 모노포스페이트 (TMP) 및 티아민 디포스페이트 (TPP)의 합성을 일으키는 각각의 효소적 단계의 중간체를 나타내는 그림. 중간체의 약어: 5-아미노이미다졸 리보뉴클레오티드 (AIR: aminoimidazole ribonucleotide), 4-아미노-2-메틸-5-(포스포옥시메틸)피리미딘 (HMP-P: 4-amino-2-methyl- 5-(phosphooxymethyl)pyrimidine), 4-아미노-2-메틸-5-(디포스포메틸)피리미딘 (HMP-PP: 4-amino-2-methyl-5-(diphosphomethyl) pyrimidine), 1-데옥시-D-자일룰로스 5-포스페이트 (DXP: 1-deoxy-D-xylulose 5-phosphate), 디하이드로글리신 (DHG: dehydroglycine), 4-메틸-5-(2-포스포옥시에틸)티아졸 (THZ- P: 4-methyl-5-(2-phosphooxyethyl)thiazole), 아데노신 트리포스페이트 (ATP: adenosine triphosphate), 아데노신 모노포스페이트 (AMP: adenosine monophosphate), S-아데노실-L-메티오닌 (SAM: S-adenosyl-L-methionine), 환원된 니코틴아마이드 아데닌 디뉴클레오티드 포스페이트 (NADPH: reduced nicotinamide adenine dinucleotide phosphate), 니코틴아마이드 아데닌 디뉴클레오티드 포스페이트 (NADP+: nicotinamide adenine dinucleotide phosphate), 환원된 페레독신 (Fdx red: reduced ferredoxin), 산화된 페레독신 (Fdx ox: oxidized ferredoxin).
도 4 IPTG 유도성 bioB 발현 플라스미드를 포함하는 △bioB 균주 (대징균 BW25113) (오른쪽 패널); 및 IPTG 유도성 프레임시프트된 bioB (조기 종결 코돈) 발현 플라스미드를 포함하는 표준 균주 (대장균 BW25113) (왼쪽 패널)의 시간 경과에 따라 측정된 세포 밀도 (OD600에서 측정됨)의 그래픽 표현 (Graphical presentation). 0.1 g/L DTB, 50 ㎍/mL 카나마이신 및 0 (점), 0.01 (삼각형), 또는 0.1 (사각형) mM IPTG를 갖는 200 ㎕ mMOPS에서 성장된 4개의 생물학적 복제물에 대하여 OD620은 멀티스칸을 사용하여 측정되고, OD600으로 전환되었다. 각 지수 성장률 값은 인접한 상자에 표시된다.
도 5 275 rpm 진탕을 하며 37℃에서 20시간 동안의 인큐베이션한 후에 (하기 기재된 비오틴 정량화하는 방법과 같음), 0 또는 0.244 ㎍ 비오틴/mL까지 증가하는 농도의 비오틴이 보충된 40 ㎍/mL 제오신을 갖는 150 ㎕ mMOPS 상에서 성장된 플라스미드 pBS451을 포함하는 대장균 BS1011의 배양물의 최종 세포 밀도 (OD600에서 측정됨)를 나타낸 산포도의 그래픽 표현. 수직의 회색 점선은 비오틴 바이오어쎄이를 위한 0.024 내지 0.24 ㎍ 비오틴/L의 최적의 농도 범위를 식별한다.
도 6 IPTG-유도성 bioB 발현 플라스미드 (pBS412)를 각각 포함하는 4개의 생물학적 복제물에서 아미노산 돌연변이를 갖는 돌연변이 IscR을 발현하는 3개의 상이한 iscR 돌연변이 균주 (BS1377, L15F), (BS1375, C92Y) 및 (BS1353, H107Y) 및 대장균 BW25113 bioB 균주 (BS1011, Ref) (균주에 대하여 표 1 참조)의 비오틴 생산을 나타내는 바 다이아그램. 균주들은 24시간 동안 37℃에서 275 rpm 진탕과 함께 0.1 g/l DTB 및 50 ㎍/mL 카나마이신을 갖는 400 ㎕ mMOPS에서 성장되었다. 바는 평균 비오틴 생산 값 (높이) 및 IPTG 유도 수준 (회색 음영)을 예시하고, 검은색 점은 개별적인 복제물 배양물로부터의 비오틴 생산을 나타내고, 수평의 점선은 참조 야생형 균주로부터의 최대 비오틴 생산을 표시한다. IPTG가 없이 배양된 경우 검출 가능한 수준의 비오틴을 생산한 균주는 없었다.
도 7 돌연변이 IscR을 발현하는 iscR 돌연변이 균주 (BS1353, H107Y), 및 대장균 BW25113 △bioB균주 (BS1011, Ref)의 세포 밀도 및 비오틴 생산의 그래픽 표현이고, 상기 각 균주는 IPTG-유도성 bioB 유전자 발현 플라스미드 (pBS412)를 포함한다. 상기 데이터는 25시간 동안 모니터링된 각각의 iscR H107Y 돌연변이 균주 (굵은 어두운 선) 및 표준 균주, 대장균 BW25113 △bioB 균주 (밝은 회색 점선)의 3개의 생물학적 복제물의 측정된 OD600 평균, 및 25시간 동안 모니터링된 각각의 iscR H107Y 돌연변이 균주 (굵은 어두운 점) 및 표준 균주, 대장균 BW25113 △bioB 균주 (밝은 회색 점)에 의한 비오틴 생산을 나타낸다. 상기 균주들은 250 mL 배플 진탕 플라스크 (baffled shake flask)에서 0.1 g/l DTB, 0.01 (A) 또는 0.5 mM IPTG (B) 및 50 ㎍/mL 카나마이신을 갖는 50 mL mMOPS에서 37℃에서 275 rpm 진탕과 함께 성장되었다. 성장률은 검정색 박스에 나타낸다.
도 8 동정된 돌연변이 균주의 iscR 유전자에서 뉴클레오티드 및 아미노산 서열 돌연변이의 위치를 나타내기 위해 주석이 달린 IscR 코딩 서열을 나타낸 그림
도 9 막대(sticks, WT 아미노산은 회색, 돌연변이 아미노산은 검정색으로 표시됨)로 표시된 L15F 및 H107Y iscR 돌연변이를 갖는 hya DNA 결합 부위 (검정색)에 결합된 IscR 이합체 (회색)의 결정 구조 (PDB entry 4HF1)를 나타낸 그림; 및 돌연변이된 잔기를 강조한 확대 이미지.
도 10 IPTG-유도성 bioB 발현 플라스미드 및 isc-오페론 (iscSUA-hscBA-fdx, iscR 유전자가 결핍된천연 대장균 isc 오페론 구조에 상응함) 또는 강한 리보좀 결합 부위 (RBS: strong ribosomal binding site)에 작동 가능하게 연결된 대장균 suf-오페론 (sufABCDSE)을 포함하는 플라스미드 및 미디엄 카피 넘버 플라스미드(p15A ori) 또는 대조군 플라스미드로부터의 T5 LacO 억제 프로모터를 포함하는 대장균 균주의 비오틴 생산을 나타내는 바 다이아그램. 상기 대조군 플라스미드는 suf- 또는 isc-오페론 대신에 IPTG-유도성 GFP를 코딩하는 유전자를 포함하였다. 각 균주의 생물학적 삼중물 (triplicates)은 100 μg/mL 암피실린 및 50 μg/mL 스펙티노마이신을 갖는 mMOPS에서 0.1 g/l (DTB)를 기질로서 제공하여 낮은 (0.01 mM IPTG) 및 높은 (0.1 mM IPTG) 유도 하에 배양되었다. 비오틴 생산이 성장 기반의 바이오어쎄이를 이용하여 평가된 후, 상기 균주들은 딥 웰 플레이트에서 24시간 동안 37℃에서 275 rpm으로 성장되었다. 바는 평균 비오틴 성장 값 (높이) 및 IPTG 유도 수준 (회색 음영)을 예시하고, 검은색 점은 개별적인 복제물로부터의 비오틴 생산을 나타내고, X표(crosses)는 OD600으로 측정된 각 균주의 종점(end-point, end) 세포 밀도를 나타낸다. 0.01 mM IPTG로 유도된 경우, 검출 가능한 수준의 비오틴을 생산한 균주는 없었다.
도 11 삼중불에서 수행된 4개의 상이한 샘플에서의 BioB 단백질 발현 수준 및 비오틴 생산의 상관관계의 그래픽 표현. 상기 균주는 pBS430 (bioB 프레임시프트 IPTG 유도성 플라스미드)을 갖는 BS1013 (대장균 BW25113, 백그라운드 균주), pBS412 (bioB IPTG 유도성 플라스미드)를 갖는 BS1011 (△bioB를 갖는 BS1013), pBS412를 갖는 BS1353 (iscR H107Y 돌연변이를 갖는 BS1011)이었다. 균주들은 0.1 g/l DTB 및 그래프에 나타낸 바와 같은 IPTG를 갖는 mMOPS에서 성장되었다.
도 12 IPTG-유도성 bioB 발현 플라스미드 및 다음 중 iscR의 게놈 변이체를 포함하는 대장균 △bioB 균주의 비오틴 생산을 나타내는 바 다이아그램: 야생형 (iscR WT), 22번 위치에서 종결 코돈으로 돌연변이된 E22* 글루탐산을 코딩하는 녹-아웃 돌연변이 (iscR KO), 92번 위치에서 시스테인으로부터 티로신으로의 치환을 코딩하는 돌연변이 (iscR C92Y). 바는 IPTG 유도의 주어진 수준 (회색의 음영)에서의 비오틴 생산 평균 값 (높이)을 예시하고, 점은 개별적인 복제물로부터의 비오틴 생산을 나타낸다. 각 균주의 생물학적 삼중물은 100 μg/mL 암피실린을 갖는 mMOPS에서 0.1 g/l DTB를 기질로서 제공하여 없는 (0 mM IPTG), 낮은 (0.01 mM IPTG) 및 낮은 (0.1 mM IPTG) 유도 하에 배양되었다. 비오틴 생산이 성장 기반의 바이오어쎄이를 이용하여 평가된 후, 각 균주는 딥 웰 플레이트에서 24시간 동안 37℃에서 275 rpm으로 성장되었다.
도 13 비오틴-오페론 플라스미드 및 다음 중 iscR의 게놈 변이체를 포함하는 대장균 △bioA △bioBFCD 균주에 의한 비오틴 생산을 나타내는 바 다이아그램: 야생형 (iscR WT), 돌연변이 iscR H107Y (107번 위치에서 히스티딘으로부터 티로신으로의 치환을 코딩함), 92번 위치에서 시스테인으로부터 티로신으로의 치환을 코딩하는 돌연변이 (iscR C92Y). 각 균주의 생물학적 사중물 (quadruplicate)은 0.1 데스티오비오틴 (DTB)을 기질로서 제공하거나 제공하지 않고 10 μg/mL 테트라사이클린을 갖는 mMOPS에서 배양되었다. 비오틴 생산이 성장 기반의 바이오어쎄이를 이용하여 평가된 후, 상기 균주들은 딥 웰 플레이트에서 24시간 동안 37℃에서 275 rpm으로 성장되었다. 바는 비오틴 생산 평균 값 (높이) 및 DTB의 공급 여부 (회색의 음영)를 예시하고, 검은색 점은 개별적인 복제물로부터의 비오틴 생산을 나타낸다.
도 14 IPTG 유도성 lipA (pBS993, 표 4 참조)를 각각 포함하는 3개의 생물학적 복제물에서 아미노산 돌연변이를 갖는 돌연변이 IscR을 발현하는 2개의 상이한 iscR 돌연변이 균주 (BS1375 C92Y) 및 (BS1353, H107Y) 및 대조군 균주 (BS1011, IscR WT) (균주에 대하여 표 1 참조)의 리포산 생산 및 생산 (X표) 24시간 후의 종점(최종) OD600을 나타내는 바 다이아그램. 균주들은 100 g/mL 암피실린, 0.1 mM 비오틴, 0.6 g/l 옥탄산 및 0.01 mM IPTG을 갖는 400 ㎕ mMOPS에서 24시간 동안 37℃에서 275 rpm 진탕과 함께 성장되었다. 바는 리포산 생산 평균 값 (높이)을 예시하고, 검은색 점은 개별적인 복제물 배양물로부터의 리포산 생산을 나타낸다. 종점 OD600이 동일하게 유지되더라도, 평균 리포산 생산은 1.79-배 증가하는 것을 볼 수 있다.
도 15 표준 균주 (대장균 BW25113), △lipA (WT, 삼각형); 및 돌연변이 iscR (C92Y)를 갖는 △lipA 균주 (C92Y, 사각형)로서, 상기 균주 모두 IPTG 유도성 lipA 발현 플라스미드 (pBS1037)를 포함하는 균주의 시간 경과에 따라 측정된, 세포 밀도 (OD620에서 측정됨)의 그래픽 표현. OD620은 0.6 g/L 옥탄산, 100 g/mL 암피실린 및 0 내지 0.04 mM IPTG (증가에 따라 회색 음영의 어둠이 증가함)을 갖는 200 ㎕ mMOPS에서 성장된 6개의 생물학적 균주 복제물에 대하여 멀티스칸을 이용하여 측정되었다. 각 생존율 (GR: growth rate)은 오른쪽에 나타낸다.
도 16 전체 티아민 경로 유전자, thiCEFSGHMD를 발현하는 플라스미드 (pBS140)를 각각 포함하는 4개의 생물학적 복제물에서 아미노산 돌연변이를 갖는 돌연변이 IscR을 발현하는 2개의 상이한 iscR 돌연변이 균주 (BS2019, C92Y) 및 (BS2020, H107Y) 및 대장균 BW25113 △thiP, thiL* 균주 (BS750, Ref) (균주에 대하여 표 5 참조)의 티아민 생산을 나타내는 바 다이아그램. 균주들은 50 ㎍/mL 카나마이신을 갖는 400 ㎕ mMOPS에서 24시간 동안 37℃에서 275 rpm 진탕과 함께 성장되었다. 바는 종점 OD600에 대하여 보정된 티오크롬 어쎄이 (티아민, TMP 및 TPP를 함유함)에 의해 측정된 바와 같은 상층액에서의 티아민 생산 평균 값 (높이)을 예시하고, 검은색 점은 개별적인 복제물 배양물로부터의 티아민 생산을 나타낸다. OD 정규화(normalized) 역가는 돌연변이 균주 (BS2019 및 BS2020)에서 표준 균주 (BS750)와 비교하여 1.43-배 향상된 것을 확인할 수 있다.
도 17 IPTG-유도성 BioB 과발현 플라스미드 pBS679 단독으로 (BS1937) 또는 FldA-Fpr의 항시적 과발현을 갖는 pBS1112 (BS2185) 또는 GFP의 항시적 과발현을 갖는 pBS1054 (BS2707)를 더한 대장균 △bioABFCD iscR H107Y (107번 위치에서 히스티딘으로부터 티로신으로의 치환을 코딩함) 균주에 의한 비오틴 생산을 나타내는 바 다이아그램. 각 균주는 100 ㎍/ml 암피실린, 기질로서 0.1 g/L 데스티오비오틴 (DTB), 및 0, 0.01, 0.025, 0.05, 0.075 또는 0.1 mM IPTG를 갖는 mMOPS에서 배양되었다. BS2185 및 BS2707을 위한 배지는 50 ㎍/ml 카나마이신을 포함하는 것을 제외하고는 동일하였다. 비오틴 생산이 성장 기반의 바이오어쎄이를 이용하여 평가된 후, 상기 균주들은 딥 웰 플레이트에서 24시간 동안 37℃에서 275 rpm 진탕과 함께 성장되었다. 바는 각각의 균주에 의한 비오틴 생산 값 (높이)을 예시한다: BS1937 (검은색 바); BS2185 (회색 바); 및 BS2707 (체크무늬 회색).
도 18 IPTG-유도성 BioB 과발현 플라스미드 pBS679를 포함하는 대장균 △bioABFCD iscR H107Y (107번 위치에서 히스티딘으로부터 티로신으로의 치환을 코딩함) 균주에 의한 비오틴 생산을 나타내는 바 다이아그램. BS2185는 FldA-Fpr의 항시적 과발현을 갖는 pBS1112를 더 포함한다. BS1937은 100 ㎍/ml 암피실린, 기질로서 0.1 g/L 데스티오비오틴 (DTB), 및 0.025 mM IPTG 유도를 갖는 mMOPS에서 배양되었다. BS2185를 위한 배지는 동일하나, 50 ㎍/ml 카나마이신을 더 포함하였다. 비오틴 생산이 성장 기반의 바이오어쎄이를 이용하여 평가된 후, 상기 균주들은 딥 웰 플레이트에서 24시간 동안 37℃에서 275 rpm과 함께 성장되었다. 어두운 회색 바는 비오틴 생산 평균 값 (높이) (BS1937 n=6 및 BS2185 n=8)을 예시하고, 밝은 회색 바는 종점 OD600을 예시한다. 검은색 점은 비오틴 생산 및 개별적인 복제물로부터의 종점 OD600을 나타낸다.
정의:
아미노산 서열 상동성: 본원에서 사용된 용어 “서열 상동성”은 실질적으로 동일한 길이의 2개의 아미노산 서열 사이의 상동성 정도의 정량적인 치수를 나타낸다. 비교되는 2개의 서열은 갭(gaps)의 삽입 또는 대안적으로, 단백질 서열의 말단에서의 절단에 의해 가능한 최상의 핏(fit)을 제공하도록 정렬되어야 한다. 서열 상동성은 ((Nref- Ndif) 100)/(Nref)로 계산될 수 있고, 상기 Ndif는 정렬된 경우의 2개의 서열에서 비-동일한 잔기의 총 수이고, Nref는 서열 중 하나의 잔기의 수이다. 서열 상동성 계산은 바람직하게는 BLAST 프로그램, 예를 들어, BLASTP 프로그램 (Pearson W.R and DJ. Lipman (1988)) (www.ncbi.nlm.nih.gov/cgi-bin/BLAST)을 사용하여 자동화된다. 다중 서열 정렬은 http://www2.ebi.ac.uk/clustalw/ 에서 이용 가능한 Thompson J., et al 1994에 의해 기술된 바와 같은 디폴트 파라미터를 갖는 서열 정렬 방법 ClustalW로 수행된다. 바람직하게, 폴리펩티드에서 하나 이상의 아미노산 잔기의 치환, 삽입, 첨가 또는 결실의 수는, 이의 비교 폴리펩티드와 비교하여, 즉, 1, 2, 3, 4, 5, 6, 7, 8, 9, 또는 10 이하의 치환, 1, 2, 3, 4, 5, 6, 7, 8, 9, 또는 10 이하의 삽입, 1, 2, 3, 4, 5, 6, 7, 8, 9, 또는 10 이하의 첨가, 및 1, 2, 3, 4, 5, 6, 7, 8, 9, 또는 10 이하의 결실로 한정된다. 바람직하게, 상기 치환은 보존적 아미노산 치환이다: 제1 군: 글리신, 알라닌, 발린, 류신, 이소류신; 제2 군: 세린, 시스테인, 셀레노시스테인, 트레오닌, 메티오닌; 제3 군: 프롤린; 제4 군: 페닐알라닌, 티로신, 트립토판; 제5 군: 아스파르테이트, 글루타메이트, 아스파라긴, 글루타민인 군의 구성원 내에서의 교환으로 제한됨.
아미노산 약어: 류신 (L), 시스테인 (C), 및 히스티딘 (H).
내인성 유전자: 숙주 박테리아와 기원이 동일한 박테리아 세포 게놈 내의 유전자 (즉 숙주 박테리아의 천연 유전자)이다. 내인성 유전자는 당업계에 알려진 도구를 사용하여 유전적으로 변형될 수 있으며, 이에 의해 유전적으로 변형된 내인성 유전자는 이것이 유래된 모체 내인성 유전자에 의해 코딩되는 폴리펩티드와 하나 이상의 위치에서 아미노산 서열이 상이한 돌연변이 폴리펩티드를 코딩한다.
게놈: 세포 또는 유기체에 존재하는 유전적 물질이다; 상기 세포 또는 유기체를 구축하고 유지하기 위해 필요한 모든 정보를 포함하는 게놈은 세포 또는 유기체 내에 존재하는 염색체(들) 및 플라스미드(들) 모두에서 유전 물질을 포함한다.
GFP: 녹색 형광 단백질.
gi 번호: 유전자정보 검색번호(genInfo identifier)는 DDBJ/EMBL/GenBank로부터의 뉴클레오티드 서열, SWISS-PROT, PIR 및 다른 많은 것들로부터의 단백질 서열을 포함하여, Entrez로 처리된 모든 서열에 NCBI에 의해 할당되는 데이터베이스 근원과 관계 없이, 특정 서열을 식별하는 독특한 정수이다.
Isc 경로: 철 황 클러스터 경로; iscR 유전자를 포함한 isc 오페론에 의해 코딩됨.
멀티스칸(Multiskan): 필터-기반의 마이크로플레이트 광도계; 600 - 620nm을 포함한, 340 내지 850 nm 범위의 파장에서 96 또는 384-웰 플레이트 포맷으로부터 흡광도를 측정하기 위함. 플레이트를 최대 50℃의 선택된 온도에서 광도계에서 인큐베이트된다. 광도계는 Thermo Scientific에 의해 제공된다.
천연 유전자 (Native gene): 숙주 박테리아와 동일한, 박테리아 세포 게놈 내에서 내인성 유전자.
비-천연 프로모터 (Non-native promoter): 본 발명의 유전자 변형 박테리아와 관련하여, 상기 세포에서 유전자 또는 전이 유전자에 작동 가능하게-연결된 프로모터이며, 상기 프로모터는 자연에서 발견된 박테리아 세포에서 상기 유전자 또는 전이 유전자에 작동 가능하게-연결된 것으로 발견되지 않을 것임.
OD (Optical Density): 광학 밀도
전이 유전자(Transgene): 게놈 공학에 의해 박테리아의 게놈 내로 도입된 외인성 유전자이다. 본 발명의 내용에서, 상기 개놈은 염색체 및 에피솜 유전자 요소를 모두 포함한다.
발명의 상세한 설명
비오틴, 리포산 및 티아민의 합성을 위한 생합성 경로의 공통적인 특징은 복잡한 라디칼-매개 분자 재배열을 촉매하기 위한 하나 이상의 SAM 또는 AdoMet 라디칼 효소에 대한 필요 조건이다. 비오틴 신타아제, 리포산 신타아제, HMP-P 신타아제, 및 티로신 리아제는 이들 경로에서 이들 필수 단계를 촉매하는 것으로 알려진 각각의 효소들이다. 비오틴 오페론의 과발현, 또는 심지어 BirA 억제자에 의한 피드백 조절에 영향을 받지 않는 돌연변이 비오틴 오페론의 사용에 의한, 대장균에서 비오틴 생합성을 증가시키기 위한 초기 시도의 실패는 성장의 강력한 억제 때문이었다 (Ifuku, 0. et al., 1995). 관찰된 성장 억제에 대하여 증거-기반의 어떠한 설명도 없는 경우; 비오틴 신타아제 과-발현의 독성을 설명할 수 있는 세포 인자를 규명하기 위한 대안적인 접근법이 필요했다.
본 발명에 의해 제공되는, 이 문제에 대한 해결책은 박테리아 세포 공장 (예를 들어, 대장균)의 세포들에서 비오틴 신타아제, 리포산 신타아제, HMP-P 신타아제 및 티로신 리아제의 발현을 증가시키기 위해 동일하게 적용 가능한 것으로 나타난다. 이 문제를 해결하기 위한 접근법은 불완전한 오류-정정(error-correcting) 폴리머라제에 의해 생성된 백그라운드 돌연변이의 축적으로 인해 진화된 게놈 다양성을 갖는 대장균 세포의 라이브러리를 생성하는 것이었다. 이러한 라이브러리의 세포를 IPTG-유도성 bioB 유전자 발현 카세트를 포함하는 플라스미드로 형질전환시켰다. 후보 돌연변이는 돌인변이 세포가 유래된 모체 대장균 균주에서 BioB 발현 독성을 유도하기에 충분한 농도에서 IPTG 존재 하에 성장할 수 있는 라이브러리 내의 세포들이었다.
선택된 BioB-발현 돌연변이 균주의 증식을 위한 유전적 기초는 전체 게놈 시퀀싱에 의해 확립되었다. 놀랍게도, 3개의 균주들은 천연 철 황 클러스터 조절 유전자 (iscR)에서 돌연변이를 갖는 것으로 밝혀졌다; 이것은 다면 발현성 전사 인자 (IscR) [서열번호 2]를 코딩한다. Fe-S 클러스터는 많은 단백질 및 필수적 효소들의 보조인자로서, 비오틴과 같은 S-합유 화합물의 합성을 위해 단독으로 필요할 뿐만 아니라, 산화환원- (redox-) 또는 철-관련 스트레스 조건에 대한 센서로서 이들에 다양한 생화학적 능력을 부여한다.
IscR은 Fe-S 클러스터 단순-단백질(holo-protein), 또는 Fe-S 클러스터가 없는 아포단백질(apoprotein)로서 2가지 상태로 존재한다. IscR의 Fe-S 클러스터의 어셈블리는 isc 오페론에 의해 코딩되는 Isc 경로에 의해 촉매된다. isc 오페론은 먼저 조절자 (IscR)를 코딩하고, 그런 다음 시스테인 디설퍼라제(desulphurase) (IscS), 스캐폴드 (IscU), A-타입 단백질 (IscA), Dnaj-유사 코-샤페론(co-chaperone) (HscB), DnaK-유사 샤페론 (HscA) 및 페레독신 (Fdx)을 코딩한다. Isc 경로는 IscR 완전효소의 어셈블리를 위해 필수적일 뿐만 아니라, 대장균에서 Fe-S 클러스터 생물발생(biogenesis)을 위한 주요한 경로이다 (도 1B).
IscR의 2가지 형태 간의 비율은 [2Fe-2S] 클러스터의 세포 수준에 의해 결정되고, 이는 결국 철- 및 산소 수준을 포함한 여러 요인에 의해 영향을 받는다 (Py, B. & Barras, 2010). 철-풍부한 조건 하에, IscR은 주로 완전 효소로 존재하고, isc 오페론의 전사 억제자로 작용한다. 그러나, 철-적은 ([2Fe-2S] 클러스터의 낮은 수준) 조건 하에, IscR은 아포-단백질 상태로 전환되고, 이는 isc 오페론의 전사를 가능하게 한다. 이의 아포-단백질 상태에서, IscR은 산화 스트레스 하에 Fe-S 클러스터 생물발생을 촉매하는 sufABCDSE 오페론의 활성인자 (activator)로서 역할을 한다.
대장균에서 2개의 Fe-S-클러스터 어셈블리의 발현을 조절하는 것 외에도, IscR은 산화 스트레스 매커니즘 (예를 들어, sodA), 특이적 및 전면적(global) 조절자 (예를 들어, yqjI 및 soxS), 아미노산 생합성 (예를 들어, argE), 및 알려지지 않은 기능을 가진 다양한 유전자와 같은 작용의 다양한 메커니즘에 관여된 >40 개의 유전자들을 조절한다. IscR의 역할은 IscR 조절 환경이 호기성 및 혐기성 조건 사이에서 변화한다는 사실에 의해 더욱 복잡해진다 (Martin, and Imlay, 2012; Giel et al., 2006).
IscR의 항상성 역할; 및 전면적 유전자 조절에서의 이의 역할의 관점에서; 이의 조절 특성의 임의의 변형의 결과는 예측할 수 없으며, 아마도 세포 대사에 대해 심오하다. 또한, 황 형성 (suf) 및 isc 경로 모두의 증가된 발현으로 인해, Fe-S 클러스터 생물발생이 증가되는 세포 조건은 축적된 Fe-S 클러스터가 팬톤 반응(fenton reactions)에 의해 퍼옥사이드 라디칼을 생성할 위험을 생성한다.
이러한 관점에서, 3개의 분리된 개별적인 돌연변이에 의해 입증된 바와 같이, IscR이 세포 BioB의 활성 및 독성에 대해 매우 중요한 것으로 발견되는 것은 매우 예상하지 못한 것이었다. 또한, IscR 단백질의 중요성은 예상치 못한 것이었고, 이는 Fe-S 클러스터를 합성하고 조립하는 증가된 능력을 제공하는 isc 오페론 또는 suf 오페론의 과-발현이 bioB를 과-발현하는 세포에서 비오틴 생산을 증가시키는 것으로 발견되지 않았기 때문이다 (실시예 1, 도 10 참조). 또한, iscR 유전자 녹-아웃에 의한 세포 iscR 조절의 제거는 세포에서 비오틴 생산을 증가시키는데 실패하였다 (실시예 1, 도 12 참조).
돌연변이 세포에서 BioB 발현의 독성을 제거하는 IscR 단백질에서의 3가지의 다른 돌연변이는 아미노산들의 단일 아미노산 치환, L15 [서열번호 16], C92 [서열번호 18] 및 H107 [서열번호 20]이었다 (도 8). 3개의 돌연변이 중 2개는 IscR의 잔기에 정확하게 상응하고, 이들 각각은 IscR 단순-단백질의 형성에 필수적인 것으로 알려져 있다. 대장균에서 관찰된 바와 같이, IscR은 특이한 Fe-S 클러스터 결찰(ligation) 메커니즘을 가짐으로써, Fe-S 클러스터 결찰에 필수적인 잔기는 H107뿐만 아니라, C92, C98, 및 C104이다. 이 비정형 결찰은 다른 Fe-S 단백질에 비해 IscR의 완전효소 상태의 낮은 안정성을 부여할 수 있고, 이는 결국 낮은 Fe-S 조건 동안 아포-단백질 상태로의 전환을 설명한다 (Fleischhacker et al., 2012).
이론에 의해 구속되고자 하는 것은 아니나, 이는 본 발명의 돌연변이 iscR 유전자를 발현하는 반면에, 철-황 클러스터 함유 유전자들(비오틴 신타아제, 리포산 신타아제, HMP-P 신타아제 및 티로신 리아제)의 어셈블리를 심지어 이들의 과-발현 동안에 촉진하는 세포에서, Fe-S 클러스터 생물발생의 항상성 조절 및 세포 성장에 필요한 전면적인 유전자 조절이 유례없이 보존됨을 시사한다.
요약하면, 본 발명자들은 Fe-S 클러스터의 결찰에 필요한 하나 이상의 아미노산 잔기의 부족을 특징으로 하는, 돌연변이 IscR 단백질을 코딩하는 돌연변이 iscR 유전자로서, 그 결과 발현된 돌연변이 IscR 단백질이 아포-단백질 형태로만 존재하도록 하는 iscR 유전자를 규명하였다. 박테리아에서 비오틴, 리포산 또는 티아민의 생산을 증가시키기 위해 노력으로, 효소를 함유하는 철-황 클러스터의 합성은 상당한 장애물을 구성하는 것으로 보여진다. 본 발명에 의해 제공된 바와 같이, 이 문제에 대한 해결책은 아포-단백질 상태로 존재하는 돌연변이 IscR 단백질을 코딩하는 유전자를 포함하는 세포 공장에서 이들 효소의 과-발현에 의해 촉진된다. 본 발명의 다양한 구현예는 하기에 보다 자세히 기술된다.
I 비오틴의 생산을 위한 유전자 변형 박테리아 세포
본 발명은 증가된 수준의 비오틴을 생산할 수 있는 유전자 변형 박테리아 세포를 제공한다. 상기 박테리아 세포는 야생형 IscR을 대체하여 돌연변이 IscR을 발현하고, 비오틴 신타아제 (EC 2.8.1.6을 갖는 비오틴 신타아제)를 코딩하는 전이 유전자를 포함하도록 유전적으로 변형된다. 선택적으로, 상기 유전자 변형 박테리아 세포는 비오틴 경로 (도 1A)의 추가적인 단계를 촉매하는 폴리펩티드를 코딩하는 하나 이상의 추가적인 전이 유전자를 더 포함할 수 있다. 비오틴 경로에서 단계들을 촉매하는 이들 폴리펩티드 수준의 증가는 박테리아 세포에서 비오틴 경로에서의 중간체 및 상기 경로의 최종 생성물 (비오틴) 모두의 합성을 향상시킨다.
유전자 변형 박테리아 세포에 의해 발현되는, 돌연변이 IscR 폴리펩티드는 폴리펩티드 백본 (아포-단백질)을 특징으로 하는 IscR 폴리펩티드 군의 야생형 구성원으로부터 유래된다. IscR 폴리펩티드 군의 야생형 구성원의 아미노산 서열은 서열번호 2, 4, 6, 8, 10, 12 및 14 중 어느 하나로부터 선택된 서열과 적어도 70, 75, 80, 85, 90, 95, 96, 98, 100% 아미노산 서열 상동성을 갖는다. 본 발명에 따른 돌연변이 IscR 폴리펩티드의 아미노산 서열은 적어도 하나의 아미노산 치환에 의해 유래된 상응하는 야생형 IscR 폴리펩티드의 아미노산 서열과 상이하다; 상기 치환은 L15X, C92X, C98X, C104X, 및 H107X로부터 선택된다; 상기 치환 아미노산인 X는 돌연변이가 유래된 야생형 IscR에서 상응하는 위치에서 발견되는 아미노산 이외의 임의의 아미노산이다.
대안적인 구현예에서, 상기 돌연변이 IscR에서 아미노산 치환은 L15X로서, 상기 X는 L 이외의 임의 아미노산이고, 보다 바람직하게 X는 페닐알라닌 (F), 티로신 (Y), 메티오닌 (M) 및 트립토판 (W)으로부터 선택되는 것인 L15X; C92X로서, 상기 X는 C 이외의 임의의 아미노산이고, 보다 바람직하게는 X는 티로신 (Y), 알라닌 (A), 메티오닌 (M), 페닐알라닌 (F) 및 트립토판 (W)으로부터 선택되는 것인 C92X; C98X로서, 상기 X는 C 이외의 임의의 아미노산이고, 보다 바람직하게는 X는 알라닌 (A), 발린 (V), 이소류신 (I), 류신 (L), 페닐알라닌 (F) 및 트립토판 (W)으로부터 선택되는 것인 C98X; Cys104X로서, 상기 X는 C 이외의 임의의 아미노산이고, 보다 바람직하게는 X는 알라닌 (A), 발린 (V), 이소류신 (I), 류신 (L), 페닐알라닌 (F) 및 트립토판 (W)으로부터 선택되는 것인 Cys104X; 및 His107X로서, 상기 X는 H 이외의 임의의 아미노산이고, 보다 바람직하게는 X는 알라닌 (A), 티로신 (Y), 발린 (V), 이소류신 (I), 및 류신 (L)으로부터 선택되는 것인 His107X로부터 선택되는 것이다. 예를 들어, 상기 돌연변이 IscR에서 아미노산 치환은 L15F, C92Y, C92A, C98A, Cys104A, H107Y, 및 H107A 중에서 선택되는 것일 수 있다.
본 발명의 유전자 변형 박테리아 세포에 의해(야생형 IscR을 대체하여) 발현되는 돌연변이 IscR은 염색체 상 또는 자기-복제 플라스미드 상에, 박테리아 세포의 게놈에 위치한, 유전적으로 변형된 유전자에 의해 코딩된다. 상기 염색체에서 유전적으로 변형된 iscR 유전자는 천연 게놈에서 야생형 iscR 유전자와 동일한 위치에 있는 게놈에 위치할 수 있다. 천연 야생형 iscR 유전자는 결실되거나 유전적으로 변형된 iscR 유전자에 의해 직접적으로 치환되므로, 본 발명의 유전자 변형 박테리아 세포의 게놈은 천연 야생형 iscR 유전자가 결여된다. 유전적으로 변형된 iscR 유전자의 발현을 구동하는 프로모터는 상기 유전적으로 변형된 iscR 유전자가 유래되거나 대체된 야생형 iscR 유전자의 천연 프로모터일 수 있다. 대안적으로, 상기 프로모터는 이종의 항시성 또는 유도성 프로모터일 수 있다. 상기 프로모터가 이종의 항시성 프로모터인 경우, 적합한 프로모터는: apFab 패밀리 [서열번호 230-232]을 포함하는 반면에, 적합한 유도성 프로모터는: pBad (아라비노스 유도성 [서열번호 233] 및 LacI [서열번호 234]를 포함한다. 적합한 종결자(terminator)는 [서열번호 235-237]를 포함하는 apFAB 종결자 패밀리의 구성원을 포함한다.
본 발명에 따른 비오틴 신타아제 (EC 2.8.1.6) 활성을 갖는 폴리펩티드는 데스티오비오틴의 비오틴으로의 전환을 촉매하는 비오틴 신타아제 활성을 갖는 폴리펩티드이다. 비오틴 신타아제의 이 군의 구성원은 광범위한 속(genera)에 속하는 박테리아에서 발견된 유전자에 의해 코딩된다. 비오틴 신타아제 활성을 갖는 폴리펩티드의 아미노산 서열은 이들 중 어느 하나로부터 선택된 서열과 적어도 70, 75, 80, 85, 90, 95, 96, 98, 100% 아미노산 서열 상동성을 갖는다: 서열번호 22 (기원: 에셔리키아 콜라이(Escherichia coli)); 서열번호 27 (기원: 캔디다투스 클로라시도박테리움 써모필룸 B(Candidatus Chloracidobacterium thermophilum B)); 서열번호 29 (기원: 스트렙토마이세스 리디커스(Streptomyces lydicus)); 서열번호 31 (기원: 파라코커스 데니트리피칸스(Paracoccus denitrificans)); 서열번호 33 (기원: 파라코커스 데니트리피칸스 PD1222); 서열번호 35 (기원: 아그로박테리움 비티스(Agrobacterium vitis)); 서열번호 37 (기원: 루에제리아 포메로이(Ruegeria pomeroyi); 서열번호 39 (기원: 아그로박테리움 파브룸(Agrobacterium fabrum)); 서열번호 41 (기원: 시멕스 렉툴라리우스의 볼바키아속 내공생자(Wolbachia endosymbiont of Cimex lectularius)); 서열번호 43 (기원: 스핀고모나스 파우치모빌리스(Sphingomonas paucimobilis)); 서열번호 45 (기원: 애시디싸이오바실러스 페리보란스(Acidithiobacillus ferrivorans)); 서열번호 47 (기원: 갈리오넬라 캡시페리포르만스(Gallionella capsiferriformans)); 서열번호 49 (기원: 랄스토니아 유트로파(Ralstonia eutropha)); 서열번호 51 (기원: 보르데텔라 파라퍼투스(Bordetella parapertussis)); 서열번호 53 (기원: 푸실리모나스 종(Pusillimonas sp.)); 서열번호 55 (기원: 케나르카이움 심비오숨 종(Cenarchaeum symbiosum sp.)); 서열번호 57 (기원: 알리사이클로바실러스 아시도칼다리우스 종(Alicyclobacillus acidocaldarius sp.)); 서열번호 59 (기원: 게오바실루스 써모글루코시다시우스(Geobacillus thermoglucosidasius); 서열번호 61 (기원: 바실러스 서브틸리스(Bacillus subtilis)); 서열번호 63 (기원: 리시니바실러스 스파이리쿠스(Lysinibacillus sphaericus)); 서열번호 65 (기원: 메틸로코커스 캡슐라터스(Methylococcus capsulatus)); 서열번호 67 (기원: 레클레르시아 아데카르복시라타(Leclercia adecarboxylata)); 서열번호 69 (기원: 크로모할로박터 살렉시젠스(Chromohalobacter salexigens)); 서열번호 71, 73, 75, 77, 79, 81, 83, 85, 87 (기원: 슈도모나스 종(Pseudomonas spp)).
유전자 변형 박테리아 세포에서 추가적인 전이 유전자에 의해 코딩되고, 및 비오틴 경로의 중간체 및 생성물 모두의 합성을 증가시키는 역할을 활성을 갖는 폴리펩티드는 다음과 같다:
a) SAM (S-아데노실메티오닌)-의존적 메틸트랜스퍼라제 (BioC; EC 2.1.1.197) 활성을 갖는 폴리펩티드; 예를 들어, 서열번호 89와 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드;
b) 7-케토-8-아미노펠라르곤산 (KAPA) 신타아제 (BioF; EC 2.3.1.47) 활성을 갖는 폴리펩티드, 예를 들어, 서열번호 91과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드;
c) 7,8-디아미노펠라르곤산 (DAPA) 신타아제 (BioA; EC: 2.6.1.62) 활성을 갖는 폴리펩티드, 예를 들어, 서열번호 93과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드; 또는 L-리신:8-아미노-7-옥소노나노에이트 아미노트랜스퍼라제 (BioK; EC: 2.6.1.105) 활성을 갖는 폴리펩티드, 예를 들어, 서열번호 97과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드;
d) 데스티오비오틴 (desthiobiotin, DTB) 신타아제 (BioD; E.C 6.3.3.3) 활성을 갖는 폴리펩티드, 예를 들어, 서열번호 95와 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드; 및 선택적으로
f) 피멜로일-[아실-운반 단백질] 메틸 에스테르 에스테라제 (BioH; EC: 3.1.1.85) 활성을 갖는 폴리펩티드, 예를 들어, 서열번호 99와 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드; 또는
g) 6-카복시헥사노에이트-CoA 리가제 (BioW; EC 6.2.1.14) 활성을 갖는 폴리펩티드; 예를 들어, 서열번호 101과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드;
비오틴 경로의 추가적인 단계를 촉매하는 폴리펩티드를 코딩하는 하나 이상의 추가적인 전이 유전자와 함께, BioB를 코딩하는 전이 유전자는 박테리아 세포 염색체 내로 또는 자기-복제 플라스미드 상에 통합되는 유전자 변형 박테리아 세포의 게놈에 위치한다. BioB 및 비오틴 경로 효소들(BioABFCD 및 H 또는 W)에서 하나 이상의 효소를 코딩하는 전이 유전자는 하나 이상의 오페론 내의 게놈에 존재할 수 있다.
하나 이상의 추가적인 전이 유전자와 함께 BioB를 코딩하는 전이 유전자의 발현을 유도하는 프로모터는 바람직하게는 이종의 항시성-프로모터 또는 유도성-프로모터일 수 있는 비-천연 프로모터이다. 상기 프로모터가 이종의 항시성 프로모터인 경우, 적합한 프로모터는 apFab 패밀리 [서열번호 230-232]를 포함하는 반면에, 적합한 유도성 프로모터는: pBad (아라비노스 유도성 [서열번호 233] 및 LacI [서열번호 234]를 포함한다. 적합한 종결자는 [서열번호 235-237]를 포함하는 apFAB 종결자 패밀리의 구성원을 포함한다. 선택된 프로모터 및 종결자는 BioB에 대한 코딩 서열; 및 BioC, BioD, BioA, BioF, 및 BioW 또는 BioH 폴리펩티드에 대한 하나 이상의 코딩 서열에 작동 가능하게 연결되는 것일 수 있고, 또한 선택된 Bio 폴리펩티드를 코딩하는 하나 이상의 오페론에 작동 가능하게 연결되는 것일 수 있다.
II 본 발명에 따른 유전자 변형 박테리아를 이용하여 비오틴을 생산하고 검출하는 방법
비오틴은 비오틴의 생합성을 위해 적합한 탄소원을 포함하고; 배양에 의해 생산된 비오틴을 최종적으로 회수할 뿐만 아니라, 성장을 지지하기에 적합한 배양 배지 내로 세포를 도입함으로써, 본 발명의 유전적 변형된 박테리아 세포(예를 들어, 유전자 변형 대장균 세포)를 사용하여 비오틴은 생산되고 수출될 수 있다.
비오틴 신타아제 (BioB)를 코딩하는 전이 유전자를 포함하는 본 발명의 유전자 변형 박테리아 세포는 공급된 탄소원이 데스티오비오틴(DTB)를 포함하는 경우, 비오틴을 증가된 수준으로 생산할 것이다. 본 발명의 유전자 변형 박테리아 세포는 BioA, BioF, BioC, BioD, 및 BioH 또는 BioW 각각을 코딩하는 전이 유전자를 추가적으로 포함하고, 이는 공급된 탄소원이 글루코스, 말토스, 갈락토스, 프럭토스, 수크로스, 아라비노스, 자일로스, 라피노스, 만노스, 및 락토스 중에서 선택되는 경우, 비오틴을 생산할 것이다 (실시예 1, 도 13).
본 발명의 유전자 변형 박테리아 세포에 의해 생산된 세포 외 비오틴을 정량화하는 방법은 실시예 1.5에 기술된다. 상기 방법은 본 발명의 세포의 배양으로부터 유래된 세포 외 증식 배지로 보충된 비오틴-결핍 증식 배지 내에 플라스미드 pBS451을 포함하는 BS1011의 비오틴-결핍된(starved) 하룻밤 동안의 배양의 성장을 측정하는 것에 기초한, 바이오어쎄이 (bioassay)이다. 도 5에 나타낸 바와 같이, 비오틴 기준(standards)의 알려진 농도 범위로 보충된 경우, 비오틴 바이오어쎄이 검정 곡선 (calibration curve)이 비오틴-결핍된 하룻밤 동안의 배양의 성장을 측정함으써 작성된다.
III 리포산의 생산을 위한 유전자 변형 박테리아 세포
본 발명은 리포산의 증가된 수준을 생산할 수 있는 유전자 변형 박테리아 세포를 제공한다. 본 발명에 따르면, 상기 박테리아 세포는 리포산 신타아제 (EC 2.8.1.8)를 코딩하는 전이 유전자를 포함할 뿐만 아니라, 야생형 IscR 대신에 돌연변이 IscR을 발현하도록 유전적으로 변형된다 (섹션 I 참조). LipA는 2개의 황 결합의 형성을 촉진함으로써 공유결합된 옥타노일-도메인을 리포일 도메인으로의 전환을 촉매한다. 선택적으로, 상기 유전자 변형 박테리아 세포는 리포산 합성 경로의 추가적인 단계를 촉매하는 폴리펩티드를 코딩하는 하나 이상의 추가적인 전이 유전자를 더 포함할 수 있고 (도 2), 보다 구체적으로는 예를 들어 LipB 유전자의 상기 코딩된 폴리펩티드 LipB; EC:2.3.1.181, 및 예를 들어 aceF 유전자의 상기 코딩된 폴리펩티드 E2; EC:2.3.1.12. 박테리아 세포에서 리포산 경로에서의 단계를 촉매하는 이들 폴리펩티드의 수준의 증가는 상기 경로의 중간체 및 최종 산물 모두의 합성을 증가시킨다. LplA, 리포에이트-단백질 리가아제 A; EC:6.3.1.20를 코딩하는 추가적인 전이 유전자는 옥타노일 모이어티의 활성화된 리포일 도메인으로의 전달을 촉매함으로써, 옥탄산이 공급된 세포에서 리포산의 합성을 촉진하는 역할을 한다.
리포산 신타아제는 광범위한 속에 속하는 광범위한 박테리아 및 진균에서 발견되는 유전자에 의해 코딩된다. 리포산 신타아제 활성을 갖는 폴리펩티드의 아미노산 서열은 다음 중 어느 하나로부터 선택된 서열과 적어도 70, 75, 80, 85, 90, 95, 96, 98, 100% 아미노산 서열 상동성을 갖는다: 서열번호 103 (기원: 에셔리키아 콜라이); 서열번호 105 (기원: 바실러스 서브틸리스); 서열번호 107 (기원: 사카로미세스 세레비제(Saccharomyces cerevisiae)); 서열번호 109 (기원: 슈도모나스 푸티다 (Pseudomonas putida); 서열번호 111 (기원: 박테로이데스 프라길리스 (Bacteroides fragilis)); 및 서열번호 113 (기원: 스트렙토마이세스 씰리칼라 (Streptomyces coelicolor)).
유전자 변형 박테리아 세포에서 추가적인 전이 유전자에 의해 코딩되고, 리포산 경로의 중간체 및 생성물 모두의 합성을 증가시키는 역할을 하는 폴리펩티드는 다음과 같다:
a) 옥타노일트랜스퍼라제 활성을 갖는 폴리펩티드 (ACP로부터 타겟 효소의 E2 서브유닛의 아포-리포일 도메인으로의 옥타닐 잔기의 이동을 위한; LipB; EC: 2.3.1.181, 예를 들어, 서열번호 115 (기원: 에셔리키아 콜라이) 또는 서열번호 117 (기원: 시겔라플렉스너리 (Shigella flexneri))과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드;
b) 피루브산 탈수소효소 (E2; EC: 2.3.1.12)의 디하이드로리포일라이신-잔기 아세틸트랜스퍼라제 성분을 포함하는 폴리펩티드, 예를 들어 서열번호 119 (기원: 에셔리키아 콜라이), 또는 서열번호 121 (기원: 클렙시엘라 옥시토카 (Klebsiella oxytoca)) 또는 서열번호 239 (하이브리드 서열(hybrid sequence))과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드.
c) 리포에이트-단백질 리가아제 A (EC:6.3.1.20) 활성을 갖는 폴리펩티드, 예를 들어 서열번호 123 (기원: 에셔리키아 콜라이) 또는 서열번호 125 (기원: 클렙시엘라 옥시토카)과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드.
리포산 경로의 추가적인 단계를 촉매하는 폴리펩티드를 코딩하는 하나 이상의 추가적인 전이 유전자와 함께 리포산 신타아제를 코딩하는 전이 유전자는 박테리아 세포 염색체 내로 또는 자기-복제(self-replicating) 플라스미드 상에 통합되는 유전자 변형 박테리아 세포의 게놈에 위치한다. LipA를 코딩하는 전이 유전자 및 리포산 경로 효소를 코딩하는 하나 이상의 전이 유전자 (IpB, IplA, 및 AceF)는 하나 이상의 오페론 내에 게놈에 존재할 수 있다.
LipA를 코딩하는 전이 유전자 및 하나 이상의 추가적인 전이 유전자의 발현을 유도하는 프로모터는 바람직하게는 이종의 항시성-프로모터 또는 유도성-프로모터일 수 있는 비-천연 프로모터이다. 상기 프로모터가 이종의 항시성 프로모터인 경우, 적합한 프로모터는 apFab 패밀리 [서열번호 230-232]를 포함하는 반면에, 적합한 유도성 프로모터는: pBad (아라비노스 유도성 [서열번호 233] 및 LacI [서열번호 234]를 포함한다. 적합한 종결자는 [서열번호 235-237]를 포함하는 apFAB 종결자 패밀리의 구성원을 포함한다. 선택된 프로모터 및 종결자는 개별적인 유전자 조절을 제공하기 위해 또는 오페론의 조절을 위해, 각각의 유전자에 작동 가능하게 연결되는 것일 수 있다.
IV 본 발명의 유전자 변형 박테리아를 이용하여 리포산을 생산하고 검출하는 방법
실시예 2 및 도 14에 예시된 바와 같이, 리포산은 본 발명의 유전자 변형 박테리아 세포(예를 들어, 유전자 변형 대장균 세포)를 이용하여 적합한 배양 배지 내로 상기 세포를 도입하고; 세포에 의해 생산된 리포산을 최종적으로 회수함으로써 생산될 수 있다.
리포산 신타아제 (LipA)를 코딩하는 전이 유전자를 포함하는 본 발명의 유전자 변형 박테리아 세포는 공급된 탄소원이 옥탄산 (OA)를 포함하는 경우 리포산을 생산할 것이다. 상기 세포는 적합한 탄소원, 예를 들어, 글루코스, 말토스, 갈락토스, 프럭토스, 수크로스, 아라비노스, 자일로스, 라피노스, 만노스, 및 락토스 중에서 선택된 탄소원으로 공급될 경우, 리포산을 생산할 것이다.
본 발명의 유전자 변형 박테리아 세포에 의해 생산된 세포 외 리포산을 정량화하는 방법은 실시예 2에 기술된다. 상기 방법은 본 발명의 세포로부터 추출된 리포산으로 보충된 최소 배지 상에서 리포산-의존적 영양요구성(auxotrophic) 대장균 균주의 성장을 측정하는 것에 기초한, 바이오어쎄이이다.
V 티아민의 생산을 위한 유전자 변형 박테리아 세포
본 발명은 티아민을 증가된 수준으로 생산할 수 있는 유전자 변형 박테리아 세포를 제공한다. 본 발명에 따르면, 상기 박테리아 세포는 야생형 IscR을 대체하여 돌연변이 IscR을 발현하고, thiC에 의해 코딩되는 HMP-P 신타아제라고도 불리는 포스포메틸피리미딘 신타아제 (EC 4.1.99.17); 또는 thiH에 의해 코딩되는 티로신 리아제 (2-이미노아세테이트 신타아제 (EC 4.1.99.19)라고도 불림)를 코딩하는 전이 유전자를 포함하도록 유전적으로 변형된다.
상기 유전자 변형 박테리아 세포는 티아민 합성 경로에서 추가적인 단계를 촉매하는 폴리펩티드를 코딩하는 하나 이상의 전이 유전자를 더 포함할 수 있다 (도 3). 박테리아 세포에서 티아민 경로에서의 단계를 촉매하는 이들 폴리펩티드의 수준의 증가는 상기 경로의 중간체 및 최종 산물 모두의 합성을 증가시킨다. 예를 들어, 상기 박테리아 세포는 다음을 코딩하는 하나 이상의 전이 유전자를 더 포함할 수 있다: ThiE 티아민 포스페이트 신타아제 (EC 2.5.1.3); [ThiS] 아데닐일트랜스퍼라제 (EC 2.7.7.73) (예를 들어, thiF 유전자에 의해 코딩됨); ThiG 티아졸 신타아제 (E.C.2.8.1.10); ThiS 황-운반 단백질; ThiD 포스포하이드록시메틸피리미딘 키나아제 (EC 2.7.4.7) 및 티아민 모노-포스페이트 포스파타제 (E.C. 3.1.3.-); ThiO 글리신 옥시다아제 (EC 1.4.3.19); 및 ThiM 하이드록시에틸티아졸 키나아제 (2.7.1.50).
HMP-P 신타아제는 광범위한 속에 속하는 광범위한 박테리아 및 진균에서 발견되는 유전자에 의해 코딩된다. HMP-P 신타아제 활성을 갖는 폴리펩티드의 아미노산 서열은 다음 중 어느 하나로부터 선택된 서열과 적어도 70, 75, 80, 85, 90, 95, 96, 98, 100% 아미노산 서열 상동성을 갖는다: 서열번호 201 (기원: 에셔리키아 콜라이); 서열번호 203 (기원: 시네코커스_이롱가투스(Synechococcus_elongatus)); 서열번호 205 (기원: 코리네박테리움 글루타미쿰 (Corynebacterium glutamicum)); 서열번호 207 (기원 캔디다투스 바우마니아 시사델리니콜라 (Candidatus Baumannia cicadellinicola)). 티로신 리아제는 2-이미노아세테이트 신타아제 (EC 4.1.99.19)라고도 불린다. HMP-P 신타아제 활성을 갖는 폴리펩티드의 아미노산 서열은 서열번호 217의 서열과 적어도 70, 75, 80, 85, 90, 95, 96, 98, 100% 아미노산 서열 상동성을 갖는다.
유전자 변형 박테리아 세포에서 하나 이상의 추가적인 전이 유전자에 의해 코딩되고, 티아민 경로의 중간체 및 생성물 모두의 합성을 증가시키는 역할을 하는 폴리펩티드는 다음과 같다:
a) [ThiS] 아데닐일트랜스퍼라제 (EC 2.7.7.73) 활성을 갖는 폴리펩티드, 예를 들어 서열번호 211과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드;
b) 티아민 포스페이트 신타아제 (EC 2.5.1.3) 활성을 갖는 폴리펩티드, 예를 들어 서열번호 209와 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드;
c) 티아졸 신타아제 (E.C.2.8.1.10) 활성을 갖는 폴리펩티드, 예를 들어 서열번호 215와 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드;
d) 포스포하이드록시메틸피리미딘 키나아제 (EC 2.7.4.7) 활성을 갖는 폴리펩티드, 예를 들어 서열번호 225와 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드;
e) 글리신 옥시다아제 (EC 1.4.3.19) 활성을 갖는 폴리펩티드; 예를 들어 서열번호 219, 221, 및 223으로부터 선택된 서열과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드;
f) ThiS 황-운반 활성을 갖는 폴리펩티드 예를 들어 서열번호 213과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드;
g) 티아민 모노-포스페이트 포스파타제 (E.C. 3.1.3.-) 활성을 갖는 폴리펩티드; 예를 들어 서열번호 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199 중 어느 하나로부터 선택된 서열과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드; 및
h) ThiM 하이드록시에틸티아졸 키나아제 (2.7.1.50) 활성을 갖는 폴리펩티드, 예를 들어 서열번호 227과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는 폴리펩티드.
바람직하게는, 상기 유전자 변형 박테리아 세포는 다음의 효소를 코딩하는 전이 유전자를 포함한다: ThiC (thiC 유전자에 의해 코딩됨); ThiD (thiD 유전자에 의해 코딩됨), ThiE (thiE 유전자에 의해 코딩됨), ThiF (thiF 유전자에 의해 코딩됨), 황-운반 단백질 (thiS 유전자에 의해 코딩됨), ThiG (thiG 유전자에 의해 코딩됨), TMP 포스파타제 (TMP 포스파타제 유전자에 의해 코딩됨); 및 ThiH (thiH 유전자에 의해 코딩됨) 또는 ThiO (thiO 유전자에 의해 코딩됨). 구현예에 따르면, 상기 세포는 효소 ThiM (ThiM 유전자에 의해 코딩됨)을 코딩하는 전이 유전자를 더 포함할 수 있다.
본 발명의 유전자 변형 박테리아에서 티아민 합성 수준은 티아민-포스페이트 키나아제를 코딩하는 내인성 thiL 유전자의 돌연변이에 의해 더 증가될 수 있다. 돌연변이 thiL 유전자는 서열번호 228의 뉴클레오티드 서열을 가지고, 모체 야생형 유전자와 비교하여 G133D 치환을 갖는 폴리펩티드 [서열번호 229]를 코딩하는 뉴클레오티드 133-135에서의 돌연변이 (GGT에서 GAC)를 갖는다.
티아민 경로에서 추가적인 단계를 촉매하는 폴리펩티드를 코딩하는 하나 이상의 추가적인 전이 유전자와 함께, thiC에 의해 코딩되는, HMP-P 신타아제 (EC 4.1.99.17); 또는 thiH에 의해 코딩되는 티로신 리아제 (EC 4.1.99.19)를 코딩하는 전이 유전자는 박테리아 세포 염색체 내로 또는 자기-복제 플라스미드 상에 통합되는 유전자 변형 박테리아 세포의 게놈에 위치한다. thiC 또는 thiH 전이 유전자 및 티아민 경로에서의 효소를 코딩하는 하나 이상의 전이 유전자는 하나 이상의 오페론 내에 게놈에 위치할 수 있다.
thiC 또는 thiH 전이 유전자 및 하나 이상의 추가적인 전이 유전자의 발현을 유도하는 프로모터는 바람직하게는 이종의 항시성-프로모터 또는 유도성-프로모터일 수 있는 비-천연 프로모터이다. 상기 프로모터가 이종의 항시성 프로모터인 경우, 적합한 프로모터는 apFab 패밀리 [서열번호 230-232]를 포함하는 반면에, 적합한 유도성 프로모터는: pBad (아라비노스 유도성 [서열번호 233] 및 LacI [서열번호 234]를 포함한다. 적합한 종결자는 [서열번호 235-237]를 포함하는 apFAB 종결자 패밀리의 구성원을 포함한다. 선택된 프로모터 및 종결자는 개별적인 유전자 조절을 제공하기 위해 또는 오페론의 조절을 위해, 각각의 유전자에 작동 가능하게 연결되는 것일 수 있다.
VI 본 발명에 따른 유전자 변형 박테리아를 이용하여 티아민을 생산하고 검출하는 방법
실시예 3 및 도 16에 예시된 바와 같이, 티아민, 티아민 모노포스페이트 (TMP) 및 티아민 디포스페이트 (TPP)는 본 발명의 유전자 변형 박테리아 세포 (예를 들어, 유전자 변형 대장균 세포)를 이용하여 적합한 배양 배지 내로 상기 세포를 도입하고; 최종적으로 티아민, 및 추가적으로는 상기 세포에 의해 생산된 TPP 및 TMP를 회수함으로써 생산될 수 있다.
HMP-P 신타아제를 코딩하는 전이 유전자를 포함하는 본 발명의 유전자 변형 박테리아 세포는 공급된 탄소원이 글루코스, 말토스, 갈락토스, 프럭토스, 수크로스, 아라비노스, 자일로스, 라피노스, 만노스, 및 락토스 중에서 선택되는 경우, 티아민, TPP 및 TMP를 생산할 것이다.
본 발명의 유전자 변형 박테리아 세포에 의해 생산된 티아민을 정량화하는 방법은 실시예 3에 기술되고; 티아민 기준과 비교한 고압력 액체 크로마토그래피 (High Pressure Liquid Chromatography)의 사용을 포함할 수 있다.
VII 비오틴, 리포산 또는 티아민의 생산을 위한 유전자 변형 박테리아 세포를 설계하는 방법
본 발명의 박테리아 세포에서 비오틴, 리포산 또는 티아민의 합성과 관련된 효소 활성을 갖는 하나 이상의 폴리펩티드를 코딩하는 하나 이상의 전이 유전자를 클로닝하고 도입하기에 적합한 통합 (Integration) 및 자기-복제 벡터는 통상의 기술자에게 상업적으로 이용 가능하고 알려져 있다 (예를 들어, Sambrook et al., Molecular Cloning : A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press, 1989 참조). 박테리아 세포는 이종 DNA의 세포로의 도입에 의해 유전적으로 조작된다. 본 발명의 박테리아 세포에서 비오틴, 리포산 또는 티아민 합성과 관련된 효소 활성을 갖는 하나 이상의 폴리펩티드를 코딩하는 유전자의 이종 발현은 실시예 1, 2 및 3에서 각각 입증된다.
본 발명의 박테리아 세포에서 비오틴, 리포산 또는 티아민 합성과 관련된 효소 활성을 갖는 하나 이상의 폴리펩티드를 코딩하는, 핵산 분자는 자기-복제 벡터에 의해 숙주 세포 내로 도입되거나, 당업계의 표준인 방법 및 기술을 사용하여 숙주 세포 게놈 내로 선택적으로 통합될 수 있다. 예를 들어, 핵산 분자는 화학적 변형 (chemical transformation) 및 전기천공법을 포함한 형질전환 (transformation), 형질도입 (transduction), 유전자총법 (particle bombardment), 등과 같은 표준 프로토콜에 의해 도입될 수 있다. 청구된 발명의 효소를 코딩하는 핵산 분자를 발현하는 것은 게놈 내로 핵산 분자를 통합함으로써 달성될 수 있다.
본 발명의 박테리아 세포에서 천연 내인성 iscR 유전자의 유전적 변형은 표준 재조합 방법을 적합한 모체 박테리아 세포에 적용함으로써, 내인성 iscR 유전자의 결실(녹아웃) 및 섹션 I에 기재된 바와 같은 돌연변이 IscR 폴리펩티드를 코딩하는 전이 유전자를 이용한 삽입/치환에 의해 수행될 수 있다 (Datsenko KA, et al. ; 2000).
비오틴, 리포산 또는 티아민의 생산을 위한, 본 발명에 따른 유전자 변형 박테리아 세포는 박테리아(bacterium)일 수 있고, 적합한 박테리아의 비-완전한(exhaustive) 리스트는 다음과 같이 제공된다: 에셔리키아 (Escherichia), 브레비박테리움 (Brevibacterium), 버크홀데리아 (Burkholderia), 캄필로박터 (Campylobacter), 코리네박테리움 (Corynebacterium), 슈도모나스 (Pseudomonas), 셀라티아 (Serratia), 락토바실러스 (Lactobacillus), 락토코커스 (Lactocooccus), 아세토박터 (Acetobacter), 아시네토박터 (Acinetobacter), 슈도모나스 (Pseudomonas) 등으로 이루어진 군으로부터 선택된 박테리아의 속에 속하는 종.
본 발명의 바람직한 박테리아 종은 에셔리키아 콜라이, 슈도모나스 푸티다, 셀라티아 마르센세스 (Serratia marcescens), 및 코리네박테리움 글루타미쿰이다.
VIII 본 발명의 유전자 변형 박테리아 세포의 비오틴 생산 능력은 증가된 전자 전달에 의해 향상된다.
산화된 [4Fe-4S]2+　클러스터를 포함하는 SAM-라디칼 철-황 클러스터 효소, 예를 들어, BioB, ThiC 및 LipA는 [4Fe-4S]+　클러스터로 환원하기 위해 전자 이동이 필요하다. 환원된 [4Fe-4S]+　클러스터만이 촉매에 필요한 SAM-라디칼을 생성할 수 있다. 전자 공여자 NADPH로부터 [4Fe-4S]2+로의 전자 이동은 플라보독신/페레독신 환원 효소 (Fpr) 및 플라보독신 (FldA) 환원 시스템에 의해 또는 피루브산-플라보독신/페레독신 산화환원 효소 시스템에 의해 매개될 수 있다.
추가적인 구현예에서, 비오틴, 리포산 또는 티아민을 생산할 수 있는 본 발명에 따른 유전자 변형 박테리아 세포는 다음의 군으로부터 선택된 하나 이상의 유전자를 더 포함한다: 플라보독신/페레독신-NADP 환원 효소 (EC:1.18.1.2 및 EC 1.19.1.1)를 코딩하는 유전자; 피루브산-플라보독신/페레독신 산화 환원 효소 (EC 1.2.7)를 코딩하는 유전자; 플라보독신을 코딩하는 유전자; 페레독신을 코딩하는 유전자; 플라보독신 및 페레독신-NADP 환원 효소를 코딩하는 유전자. 상기 하나 이상의 유전자에 작동 가능하게-연결된 프로모터는 상기 박테리아에서 상기 하나 이상의 유전자의 발현을 증가시킬 수 있다; 상기 하나 이상의 유전자는 천연 유전자 또는 전이 유전자일 수 있다. 바람직하게, 상기 작동 가능하게-연결된 프로모더는 본 발명의 유전자 변형 박테리아가 유래된 모체 박테리아 보다 더 높은 수준으로 상기 박테리아에서 상기 하나 이상의 유전자의 발현을 증가시킨다. 바람직하게, 본 발명의 유전자 변형 박테리아 세포는 플라보독신/페레독신-NADP 환원 효소 (EC:1.18.1.2 및 EC 1.19.1.1)를 코딩하는 유전자 및 플라보독신을 코딩하는 유전자; 또는 플라보독신 및 페레독신-NADP 환원 효소 모두에 대한 코딩 서열을 포함하는 단일 유전자를 포함한다. 추가적으로 상기 유전자 변형 박테리아 세포는 페레독신을 코딩하는 유전자를 더 포함할 수 있다.
본 발명의 유전자 변형 박테리아 세포에서 전자 이동 경로의 구성요소를 발현하는 유전자의 과발현은 이들의 SAM-라디칼 철-황 클러스터 효소의 세포 활성을 증가시킨다 (본 발명의 비오틴-생산 세포에 대한 실시예 4에 예시된 바와 같음).
바람직하게, 본 발명의 유전자 변형 박테리아 세포에서 천연 유전자 또는 전이 유전자에 의해 코딩되는 폴리펩티드는 플라보독신/페레독신 환원효소 (EC:1.18.1.2 및 EC 1.19.1.1) 활성을 가지고, 이는 다음 중 어느 하나로부터 선택된 서열과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는다: 서열번호 241 (기원: 대장균으로부터 fpr 유전자); 서열번호 243 (기원: 바실러스 서브틸리스 168로부터 yumC 유전자); 서열번호 245 (기원: 슈도모나스 푸티다 KT2440로부터 fpr-I 유전자); 서열번호 247 (기원: 스트렙토마이세스 베네주엘라 ATCC 10712 -로부터 SVEN_0113 유전자); 서열번호 249 (기원: 코리네박테리움 글루타미쿰 ATTCC 13032로부터 Cgl2384 유전자), 및 서열번호 251 (기원: 스핑고박테리움 종 (Sphingobacterium sp.) JB170으로부터 SJN15614.1 유전자).
바람직하게, 본 발명의 유전자 변형 박테리아 세포에서 천연 유전자 또는 전이 유전자에 의해 코딩되는 폴리펩티드는 피루브산-플라보독신/페레독신 산화 환원 효소 (EC 1.2.7) 활성을 가지고, 이는 다음 중 어느 하나로부터 선택된 서열과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는다: 서열번호 253 (기원: 대장균 K12 MG1655로부터 YdbK 유전자); 서열번호 255 (기원: 지오박터 설퍼레두신스 (Geobacter sulfurreducens) AM-1으로부터 por 유전자); 서열번호 257 (기원: 스트렙토마이세스 프라텐시스 (Streptomyces pratensis) ATCC 33331로부터 Sfla_2592 유전자; 서열번호 259 (기원: 프로피오니박테리움 프레우덴레이치트 (Propionibacterium freudenreichit) DSM 20271로부터 RM25_0186 유전자); 서열번호 261 (기원: 시네코시스티스 종 (Synechocystis sp.) PCC 6803으로부터 nifJ 유전자)
바람직하게, 본 발명의 유전자 변형 박테리아 세포에서 천연 유전자 또는 전이 유전자에 의해 코딩되는 폴리펩티드는 플라보독신이고, 이는 다음 중 어느 하나로부터 선택된 서열과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는다: 서열번호 263 (기원: 대장균 K12 MG1655로부터 fldA 유전자); 서열번호 265 (기원: 대장균 K12 MG1655로부터 fldB 유전자); 서열번호 267 (기원: 바실러스 서브틸리스 168로부터 ykuN 유전자); 서열번호 269 (기원: 시네코시스티스 종 PCC 6803로부터 isiB 유전자; 서열번호 271 (기원: 스트렙토마이세스 베네주엘라 ATCC 10712로부터 wrbA 유전자); 서열번호 273 (기원: 메타노코커스 아이올리쿠스 난카이-3 (Methanococcus aeolicus Nankai-3)으로부터 PRK06242 유전자).
바람직하게, 본 발명의 유전자 변형 박테리아 세포에서 천연 유전자 또는 전이 유전자에 의해 코딩되는 폴리펩티드는 페레독신이고, 이는 다음 중 어느 하나로부터 선택된 서열과 80, 85, 90, 95 또는 100% 서열 상동성을 갖는 아미노산 서열을 갖는다: 서열번호 275 (기원: 대장균으로부터 fdx 유전자); 서열번호 277 (기원: 바실러스 서브틸리스 168로부터 fer 유전자); 서열번호 279 (기원: 코리네박테리움 글루타미쿰 ATTCC 13032로부터 fdxB 유전자); 서열번호 281 (기원: 시네코시스티스 종 PCC 6803로부터 fdx 유전자); 서열번호 283 (기원: 스트렙토마이세스 베네주엘라 ATCC 10712로부터 SVEN_7039 유전자); 서열번호 285 (기원: 메타노코커스 아이올리쿠스 난카이-3 으로부터 fdx 유전자).
유전자 발현을 증가시킬 수 있는 프로모터는 상기 박테리아에서 전자 이동 경로의 폴리펩티드를 코딩하는 천연 유전자 또는 전이 유전자에 작동 가능하게-연결된 경우 바람직하게 비-천연 프로모터이다. 상기 프로모터는 항시성 apFab309 프로모터 패밀리 [서열번호 230-232]의 구성원일 수 있다. 바람직하게 상기 비-천연 프로모터는 상기 천연 유전자 또는 전이 유전자에 작동 가능하게-연결된 경우 상기 유전자 변형 박테리아에서 이 박테리아가 유래된 모체 박테리아 보다 높은 수준까지 상기 코딩된 폴리펩티드의 발현을 증가시킨다. 상기 천연 유전자 또는 전이 유전자에 작동 가능하게-연결될 수 있는 적합한 종결자는 apFAB378 종결자 패밀리 [서열번호 235-237]를 포함한다.
실시예
실시예 1: 비오틴 생산을 향상시킬 수 있는 유전자 변형 대장균 균주의 동정 및 특성화
1 방법
1. 1 : 실시예에서 사용된 하기 에셰리키아 균주를 하기 열거한다.
균주
명칭 설명
BS1013 하기 유전자형을 갖는 E. coli K-12 BW25113 모체 균주:rrnB3 △lacZ4787 hsdR514 △(araBAD)567 △(rhaBAD)568 rph-1
BS1011 E coli K-12 BW25113로부터 유래된 △bioB 1(JW0758-1)
BS1353 iscR 유전자에 H107Y 돌연변이를 포함하는 BS1011 파생체(derivative)
BS1113 IPTG-유도성 BioB 발현을 제공하는 pBS412 플라스미드를 포함하는 BS1011 파생체
BS1375 iscR 유전자에 C92Y 돌연변이를 포함하는 BS1011 파생체
BS1377 iscR 유전자에 L15F 돌연변이를 포함하는 BS1011 파생체
1△bioB 유전자의 결실 전 뉴클레오티드 서열은 서열번호 21이었다.
1.2 : 실시예에서 사용된 하기 플라스미드를 하기 나열한다.
플라스미드
명칭 설명
pBS412 T5 lacO 억제 프로모터(repressed promoter)로부터의 BioB [서열번호 22] 과발현 플라스미드 (kanR, SC101)
pBS430 T5 lacO 억제 프로모터 [서열번호 25]로부터의 bioB　1(kanR, SC101)에서 초기 프레임 시프트 돌연변이를 갖는 pBS412
pBS451 항시적으로 발현되는 GFP [서열번호 287] (zeoR, p15A)
pBS281 미디엄 카피 넘버 플라스미드(medium copy number plasmid) (p15A ori)에서 복제된 IPTG 유도성 T5 프로모터로부터의 E. coli isc 오페론 (iscSUA-hscBA-fdx)
pBS282 미디엄 카피 넘버 플라스미드 (p15 ori)에서 복제된 IPTG 유도성 T5 프로모터로부터의 E. coli suf 오페론 (sufABCDSE)
pBS231 IPTG 유도성 T5 프로모터로부터 sfGFP 단백질을 코딩하는 유전자를 발현하는 미디엄 카피 넘버 플라스미드 (p15 ori)
pBS936 bio 오퍼레이터 위치에 "타입 9(type 9)" 돌연변이를 갖는 E coli 유래의 천연 비오틴-오페론 (Ifuku et al., 1993)
1: bioB 프레임시프트(frameshift) 유전자의 뉴클레오티드 서열은 서열번호 23을 갖는다.
1.3 배지 및 첨가제:
각각의 실시예에서 사용된 증식 배지(mMPOS)는 다음의 조성물을 갖는다: 1.32 mM K2HP04; 2 g/l D-글루코스; 0.0476 mg/l 판토텐산 칼슘; 0.0138 mg/l p-아미노벤조산; 0.0138 mg/l p- 하이드록시벤조산; 0.0154 mg/l 2,3-디하이드록시벤조산, 및 1x 변형된 MOPS 버퍼.
10 x 변형된 MOPS는 0.4 M MOPS (3-(N-모르폴리노)프로판 설폰산 (3-(N-morpholino)propane sulfonic acid)); 0.04 M 트리신(Tricine); 0.1 mM FeS047H20; 95 mM NH4CI; 2.76 mM K2S04; 5 μΜ CaCI22H20; 5.25 mM MgCI2; 0.5 M NaCI; 및 미량영양소 스톡 용액(micronutrient stock solution)의 5000x 희석물을 포함한다.
미량영양소 스톡 용액:
Figure pct00001
하기의 항생제 스톡(stocks)이 적용되었다: 1000x 희석물을 수득하기 위해 표시된 바와 같이 증식 배지에 첨가된; 암피실린 (amp, 100 mg/mL), 카나마이신 (kan, 50 mg/mL), 제오신(zeocin, zeo, 40 mg/mL).
1.4 대장균 균주 라이브러리의 구축:
진화된 게놈 다양성을 갖는 대장균 라이브러리는 세포를 kan으로 보충된 mMOPS 배지(mMOPS-kan)에서 하룻밤 동안 정치 배양하고, mMOPS-kan에서 생성된 배양물의 100x 희석물을 제조하고, 하룻밤 동안의 배양 및 희석의 연속적인 단계를 5회 반복함으로써 대장균 균주 BS1011의 세포로부터 유래된다. 이 과정은 불완전한 오류-정정 폴리머라제에 의해 생성된 백그라운드 돌연변이의 축적을 허용함으로써 유전적 다양성을 만든다. 배양 및 희석의 각 라운드 후에, 증가된 BioB 발현을 견디도록 적응된 세포의 진화를 검출하기 위해, 세포 배양물의 샘플은 IPTG를 갖는 mMOPS 플레이트 상에 플레이팅하였다(하기 참조). 이어서, 각 라이브러리의 세포는 BioB 과-발현 플라스미드, pBS412로 형질전환되었다.
1.5 돌연변이 균주의 선별
IPTG를 0, 0.0001, 0.001, 0.01, 0.1 및 1 mM의 농도로 포함하는 mMOPS (Ø=9츠)를 포함하는 일련의 1.5% 아가 플레이트 상에서 pBS412를 포함하는 BS1011의 mMOPS-kan에서 하룻밤 동안(o/n)의 배양으로부터 유래된 104, 105, 106　및 107 각각의 세포를 플레이팅함으로써, 선별 어쎄이(selection assay)가 개발되었다. 이어서, 상기 플레이트는 37℃에서 최대 36시간 동안 인큐베이션되었고, 세포 성장은 간격을 두고 평가되었다. 이러한 조건들 하에, 0.1 mM IPTG으로 pBS412로부터의 BioB 발현의 유도는 최대 10Λ5 세포의 성장을 억제하는 것으로 발견된 반면에, 1 mM IPTG으로의 유도는 싱글 페트리 디쉬 상에 플레이팅된 경우에 적어도 10Λ7 세포의 성장을 억제하였다. 10Λ5 세포의 세포 집단에 대한 1 mM IPTG로의 유도를 포함한 선택압(selection pressure)은 BioB 발현에 대한 더 높은 강건함을 갖는 균주를 식별하기 위해 최적인 건으로 발견되었고; 이에 따라 다음과 같이 실시되었다:
1) 섹션 1.4에 기재된 바와 같이, 각 라이브러리로부터 약 105 세포는 각 mMOPS-kan-1 mM IPTG 아가 플레이트 상에 플레이팅되었고, 최대 24시간 동안 37℃에서 인큐베이션되었다.
2) 단일 콜로니는 전-배양물(pre-cultures)을 생산하기 위해 mMOPS-kan 액체 배지에서 성장되었고, 이어서 전-배양물은 0.00, 0.01 또는 0.1 mM IPTG로 보충된 mMOPS-kan에서 수행된 비오틴 바이오어쎄이를 이용함으로써 이들의 비오틴 생산에 대해 평가되었다 (하기 섹션 1.6에 기재된 바와 같음). 각각의 전-배양물의 세포는 20% 글리세롤에서 글리세롤 스톡으로 보존되었다.
3) 1.5 mg 이상의 비오틴/l (세포 외 비오틴으로 검출됨)을 생산하는 콜로니는 mMOPS-kan 아가 플레이트 상에 재-배열되었고 (re-streaked), 최대 24시간 동안 37℃에서 인큐베이션되었고, 이어서 생물학적 복제물에서 비오틴 생산에 대하여 재-바이오어쎄이되었다 (re-bioassayed) (하기 섹션 1.6에 상세히 기재된 바와 같음).
4) 선별된 비오틴 과-발현 균주의 세포는 다음과 같이 평가되었다:
a) 모체 균주 BS1011의 게놈과 비교하여 선별된 균주의 세포의 게놈에서의 유전적 돌연변이를 식별하기 위해 전체 게놈 시퀀싱을 선별된 균주의 세포로부터 분리된 DNA 상에서 다음과 같이 수행하였다: 선별된 균주는 5-10 mL mMOPS-kan에서 성장되었고, 상기 세포는 이후 수확되었다; 게놈 DNA는 Invitrogen Purelink 게놈 DNA 추출 키트: (https://www.thermofisher.com/order/cataloq/product/K182001)를 이용하여, 수확된 세포로부터 분리되었다; 상기 추출된 DNA는 전체 게놈 시퀀싱에 적용되었다.
b) pBS412 플라시므디의 선택된 균주의 세포를 양생(curing)하는 단계; 이어서 상기 양생된 균주의 세포를 pBS412 플라스미드로 재-형질전환시키는 단계; 및 최종적으로 상기 형질전환된 균주의 세포의 배양물의 비오틴 생산에 대한 바이오어쎄이를 다시하는 단계. pBS412 플라스미드의 세포를 양생하는 단계는 항생제를 포함하지 않고 1 mM IPTG를 갖는 풍부한(rich) 루리아 브로스(LB: Luria Broth) 배지에서 세포를 37℃에서 하룻밤 동안 증식시키고; 생성된 배양물의 세포를 LB 아가 플레이트 상에 배열(streaking out)시키고 37℃에서 하룻밤 동안 인큐베이션 함으로써 수행되었다. 아가 플레이트로부터 단일 콜로니는 50 μl LB 배지에서 희석되었고, 5 μl는 37℃에서 하룻밤 동안 인큐베이션된 LB 및 LB-amp 아가 플레이트 상에 점을 표시하기 위해 사용되었다. LB-amp 플레이트 상이 아닌, LB 플레이트 상에서 성장된 이들 단일 콜로니는 재-형질전환을 위해 양성된 균주로 사용되는 단일 콜로니를 수득하기 위해 LB 플레이트 상에 재-배열되었다.
생물학적 복제물에서 측정된 형질전환된 균주의 세포에 의한 비오틴 생산 (하기 섹션 1.6에 자세히 기재됨. 간단히, 비오틴 생산은 0.00, 0.01 또는 0.1 mM IPTG로 보충된 mMOPS-kan에서 생물학적 복제물에 대해 재-평가되었다. 동시에, 각각의 형질전환된 균주의 세포의 성장률은 멀티스칸 FC에서 24시간의 기간 동안의 혐기성 성장을 위해 “빠른 진탕(fast shaking)”으로 37℃에서 투명한 통기성 밀봉으로 밀봉된 미량 정량판(microtiter plate)에서 200 μL mMOPS-kan 배지에서 측정되었다. 세포 성장은 30분 마다 OD620을 측정함으로써 모니터링되었다.
1.6 비오틴 생산을 정량화하기 위한 바이오어쎄이
전-배양물은 96 딥(deep)-웰 플레이트에서 400 μL mMOPS-kan에서 선별된 단일 세포 콜로니로부터 각각 제조되었고, 16-18시간 동안 275 rpm에서 진탕하면서 37℃에서 인큐베이션되었다. 생산 배양물(production cultures)은 ~0.03의 초기 OD600을 제공하기에 충분한 전-배양물의 4 μL을 갖는, 96 딥-웰 플레이트에서, 0.1 g/L 데스티오비오틴으로 보충된 400 μL mMOPS-kan을 접종하고, 선택적으로 최종 농도가 최대 1 mM까지 IPTG를 포함함으로써 생산되었다. 이어서, 배양물은 24시간 동안 275 rpm 진탕으로 37℃에서 성장되었다. 배양물의 OD600을 측정한 후 8분 동안 4000 G에서의 원심분리에 의해, 96 딥-웰 플레이트의 세포는 펠렛화(pelleted)되었다. 각 배양물 상층액으로부터의 상층액은 초순수(ultrapure water, Mili-Q)에서 0.05 nM 내지 0.50 nM 비오틴의 농도 범위까지 희석되었다. 동시에, 0.1 nM (0. 024 μg 비오틴/L) 내지 1 nM (0.24 μg 비오틴/L)의 농도 범위에서 >5 비오틴 표준물(biotin standards)은 Milli-Q 물에서 제조되었다. 각 희석된 상층액의 15 μL 및 각각의 비오틴 표준은 미량정량판의 웰에 첨가되었다; 상기 각 웰은 플라스미드 pBS451을 포함하는 BS1011의 비오틴-결핍된 하룻밤 동안의 배양물의 135 μL를 포함하고, 상기 하룻밤 동안의 배양물은 제오신이 보충된 mMOPS에서 0.01의 초기 OD620까지 희석되었다. 상기 플레이트는 통기성 밀봉으로 밀봉되었고, OD600이 측정되기 전에 20시간 동안 275 rpm 진탕으로 37 ℃에서 인큐베이션되었다. 비오틴 표준물의 범위를 사용하여, 이 바이오어쎄이로 수득된 비오틴 바이오어쎄이 검정 곡선은 도 5에 나타낸다.
1.7 게놈 돌연변이의 동정
모든 차세대 염기서열 분석(NGS: Next-Generation Sequencing) 데이터의 경우, CLC 게놈 워크벤치 버전 9.5.3 (CLC genomic workbench version 9.5.3, Qiagen에 의해 제공됨)이 모체 균주 게놈과 비교하여 선별된 균주의 세포의 게놈에서의 돌연변이(변이체 검출에 의한 단일 또는 일부 치환, 결실 또는 삽입 및 인델(InDels) 및 구조적 변이체에 의한 더 큰 삽입/결실)를 동정하기 위해 사용되었다. 80%의 컷-오프는 시퀀싱 과정에 의해 도입된 잘못된 뉴클레오티드로부터의 게놈 돌연변이와 구별하기 위해, 돌연변이가 제공된 박테리아 균주의 세포로부터 분리된 DNA 분자 (게놈) 집단의 85% 이상에서 존재해야 한다는 것을 의미하는 "유의미한 돌연변이”로 정의하는데 사용되었다.
NCBI의 게놈 수탁 번호 CP009273은 참조 서열로서 사용되었고, 시퀀싱에 의해 서열이 입증된Keio △bioB 흉터 돌연변이 (scar mutation)를 고려하였다.
1.8 iscR 돌연변이의 프로테오믹스(proteomics) 랜드스케이프(landscape)의 특성화
1 mM IPTG 유도에서의 BS1353 + pBS412뿐만 아니라 0.025 mM IPTG 유도 수준에서의 BS1013 + pBS430, BS1011 + pBS412 및 BS1353 + pBS412의 단백질 함량은 LC-MS 및 효율적인 단백질 추출을 조합한 최근에 개발된 접근 방법에 의해 측정되었다 (Schmidt et al, 2015). 2.0% FDR의 펩티드 역치(threshold)에 따른 분석을 위해 식별되는 펩티드의 최소 개수로서, 3개의 펩타이드가 선택되었다. 단백질 발현에서 유의적인 변화는 스캐폴드 뷰어 4.7.5 (Scaffold Viewer 4.7.5)를 사용한 다중 테스팅에 대한 Benjamini-Hochberg 보정을 통한 분산 분석 (ANOVA, Analysis of Variance)에 기초하여 0.5% 신뢰 구간으로 보고된다.
0.5의 OD600에 도달할 때까지, 약 10 세대 동안의 IPTG 유도로 mMOPS에서 성장되었다. 108　세포는 최대 속도에서 4℃에서의 원심분리에 의해 수확되었고; 아이스-콜드 PBS 버퍼에서 1회 세척되었고; 최대 속도에서 4℃에서의 원심분리에 의해 재-펠렛화되고 PBS 버퍼를 제거한 후 액체 질소에서 순간-동결 (snap-frozen)되었다.
2. 결과
2.1 BioB의 과발현은 유독하다
저-카피 플라스미드로부터의 고유한 비오틴 신타아제 유전자 (bioB)를 보유하지만, IPTG-유동성 프레임시프트된 대장균 bioB 유전자(조기 종결 코돈으로 인해 비-기능성 비오틴 신타아제를 코딩함)를 발현하는 대장균은 IPTG가 있거나 없는 mMOPS-kan 배지에서 호기성으로 성장할 수 있다 이는 대장균 BW25113, BioB 프레임시프트 돌연변이의 지수 성장곡선을 나타낸 도 4 (왼쪽 패널)에 예시된다. 반대로, 대장균 녹-아웃 균주 △bioB에서 기능성 비오틴 신타아제 유전자 (bioB)의 과-발현은 성장에 유독하여, 유도기 (lag phase)에서 매우 유의적인 확장을 야기한다. 이는 저-카피 플라스미드 (Sc101 복제 기점) 상에서 IPTG-유도성 T5 프로모터로부터 대장균 bioB 유전자를 발현하는 대장균 녹-아웃 균주 △bioB의 성장을 나타낸 도 4 (오른쪽 패널)에 예시된다. 도 4에 나타낸 바와 같이, IPTG 수준의 증가에 반응한 bioB 발현의 증가의 유도는 (회색의 어둠) 유도기에 유의적으로 영향을 미치는 반면, 성장률은 약간 영향을 받는다 (검정색 박스).
2.2 향상된 비오틴 생산 역가 (production titers)를 갖는 iscR 돌연변이 균주의 분리
진화된 게놈 다양성을 갖는 대장균 라이브러리 (섹션 1.4 및 1.5 참조)는 bioB 유전자 발현에 대한 향상된 내성 및 증가된 비오틴 생산을 갖는 균주에 대해 스크리닝되었다. 선별된 균주의 전체 게놈 시퀀싱으로 3개의 특이한 돌연변이를 동정하였고, 이들 각각은 L15F, C92Y 및 H107Y 중에서 하나의 아미노산 치환을 갖는 iscR 폴리펩티드를 코딩하는 철-황 클러스터 조절자 (iscR) 유전자를 포함하고, 상기 코딩된 조절자의 아미노산 서열은 각각 서열번호 16, 18 및 20이다. 섹션 1.6 (및 도 5)에 기재된 바와 같이, 비오틴 생산 수준은 바이오어쎄이를 이용하여 측정되었다. 대장균 BW25113 △bioB 표준 균주 (reference strain)뿐만 아니라, 각각의 iscR 돌연변이 균주에 대한 비오틴 생산 역가는 2개의 상이한 IPTG 농도 수준의 부재 또는 존재에서 (증가된 IPTG는 더 진한 회색임) 0.1 g/l DTB로 보충된 mMOPS에서 성장된 4개의 생물학적 복제물 (검정색 점)에 대하여 도 6에 나타낸다. 표준 균주에서 비오틴 생산은 표준 균주의 성장에 유독한 IPTG 수준에 상응하는 0.01 mM 이상의 IPTG 수준에서 억제되었고 (도 4 참조), 반면에 iscR 돌연변이 ?누는 0.01 - 0.1 mM IPTG에서 성장하였고 비오틴을 생산하였다. 3개의 iscR 돌연변이 균주 모두 0.01 mM의 IPTG 농도에서 표준 균주 (점선)에 비해 약 1.5배 더 많은 비오틴을 생산한다. iscR 돌연변이 균주는 0.1 mM의 IPTG 농도에서 표준 균주의 가장 높은 생산 역가 (~ 1.5 mg biotin/l)에 비해 최대 2배 더 많이 (~3.2 mg 비오틴/l) 생산한다.
2.3 IscR H107Y 돌연변이 균주에서의 비오틴 생산 및 성장
iscR (H107Y) 돌연변이 균주의 성장 특성 (growth profile) 및 비오틴 생산 역가는 2개의 상이한 IPTG 유도 수준 (도 7A에서 0.01 mM) 및 0.05 mM (도 7B)에서 250 mL 진탕-플라스크 실험에서 0.1 g/l DTB로 보충된 50 mL mMOPS에서 특성화되었다. 낮은 IPTG 수준에서 (도 7A), IscR 돌연변이 균주 (어두운 회색) 및 표준 균주 (밝은 회색)는 ~ 1.1 mg 비오틴/l의 최종 역가로 성장 및 비오틴 생산 역가에 대하여 유사하였다. 그러나, 높은 IPTG 유도 수준에서 (도 7B에서 0.5 mM) 표준 균주 (밝은 회색)의 성장은 심각하게 억제되었던 반면에, IscR 돌연변이 균주는 낮은 IPTG 유도 수준에서와 동일한 성장 특성을 보유하였다. 또한, IscR 돌연변이 균주의 비오틴 생산 역가는 25시간의 성장 후에 최대 ~2.2 mg 비오틴/l까지, 약 2배로 증가되었다.
2.4 IscR 돌연변이의 작용의 메커니즘
도 6에 나타낸 바와 같이, 향상된 비오틴 내성 표현형은 동정된 3개의 IscR 돌연변이 균주 모두에 대하여 명확하게 입증되었다. C92 돌연변이 (C92Y)의 비오틴 내성을 향상시키는 능력은 IscR의 [Fe-S] 클러스터 결합 특성에서 C92의 역할에 의한 것으로 제안된다. C92Y 돌연변이로 인한 [Fe-S] 클러스터 결합 특성의 손실은 IscR의 Isc-오페론 억제 반응을 불활성화시키는 것으로 제안된다. 동시에, C92Y 돌연변이 IscR에서 IscR의 프로모터 기능은 온전하게 유지되어, 다중 세포 과정에 필수적인 다른 경로를 활성화시키는데 그 기능을 유지하는 것으로 제안된다. IscR의 [Fe-S] 클러스터 결합 특성을 제공하는 유사한 필수적 역할은 H107에 기인하고; 여기에서 H107Y 돌연변이는 대장균에서 비오틴 내성을 유사하게 향상시킬 수 있다. IscR에서 L15F 또한 철-황 클러스터 결합을 방해하여, 철-황 클러스터 고갈을 부분적으로 극복하는 것으로 제안된다. 도 9는 DNA에 결합될 때 L15 및 H107의 위치를 나타내고 (hya, PDB 엔트리 4HF1), L15는 IscR 각각의 서브유닛의 내부에 위치함을 알 수 있다. 페닐알라닌은 류신보다 상당히 큰 아미노산이고, 단백질의 3차원 폴딩을 방해할 수 있다.
2.5 대장균 균주 단독에서의 isc-오페론 또는 suf 오페론의 과발현은 비오틴 생산을 향상시키기에 충분하지 않다.
isc-오페론 (iscSUA-hscBA-fdx, iscR 유전자가 제외된 천연 대장균 오페론 구조와 상응함) 또는 suf-오페론 (sufABCDSE, 천연 대장균 오페론 구조와 상응함)의 대장균에서의 비오틴 생산에 대한 직접적인 효과를 측정하기 위해, 각각의 오페론은 강한 RBS 및 IPTG 유도성 T5 프로모터의 조절 하에 배치된 미디엄 카피 넘버 플라스미드 (p15A ori) 내로 클로닝하였다. isc- 또는 suf-오페론 대신에 슈퍼 폴더 녹색 형광 단백질 (sfGFP: super folder Green Fluorescent Protein)을 코딩하는 유전자를 포함하는 플라스미드는 대조군으로 적용되었다. 각각의 플라스미드는 IPTG-유도성 bioB 발현 플라스미드를 포함하는 대장균 균주의 세포 내로 형질전환되었다. IPTG-유도성 bioB 발현 플라스미드 뿐만 아니라, 1) IPTG-유도성 isc-오페론, 2) IPTG-유도성 suf-오페론 또는 3) IPTG-유도성 GFP (대조군): 중 하나를 포함하는 생물학적 3중 (triplicate) 콜로니는 100 μg/mL 암피실린 및 50 μg/mL 스펙티노마이신을 갖는 400 L mMOPS에서 낮은 (0.01 mM IPTG) 및 높은 (0.1 mM IPTG) 유도 하에서 배양한 후, 비오틴 생산에 대하여 분석되었다 (섹션 1.5에 기재된 바와 같음).
그래프 (도 10)으로부터, 모든 균주에서 비오틴 생산은 IPTG-유도성임에도 불구하고; 검출 가능한 비오틴 생산 수준에 도달하기에 필요한 IPTG 농도는 도 6에 나타낸 표준 및 돌연변이 iscR 균주와 비교하였을 때, 0.01 mM IPTG 내지 0.1 mM IPTG로 증가되었다. 또한, 비오틴 생산 역가는 isc- 또는 suf-오페론의 과발현에 의해 도 10의 sfGFP 균주와 비교하였을 때, 현저히 감소되었다. 또한, isc-오페론의 과발현은 suf-오페론의 과발현 보다 훨씬 더 비오틴 생산을 억제하였다. iscR에 싱글 포인트 돌연변이 (single point mutation)를 갖는 돌연변이 균주에서의 비오틴 생산의 관찰된 증가와 함께, 이들 균주에서 isc-오페론의 결과적인 억제-해제(de-repression)는 이들 균주에서의 개선된 비오틴 생산의 유일한/주요한 원인일 가능성은 낮다.
2.6 BioB 단백질 함량은 비오틴 생관과 관련이 있다
야생형 및 돌연변이 백그라운드 균주에서 BioB 과발현의 분자적 효과를 조사하기 위해, 야생형 백그라운드 균주: BS1013 보유 (holding) pBS430; 비오틴 생산 플라스미드를 갖는 야생형 iscR 균주: BS1011 보유 pBS412; 및 bioB 생산 플라스미드를 갖는 돌연변이 iscR 균주: BS1353 보유 pBS412에 대하여 프로테오믹스 측정이 수행되었다. 모든 균주는 0.1 g/L DTB 및 0.025 mM IPTG로 mMOPS에서 성장되었다. 후자 균주는 1 mM IPTG 유도에서 추가로 성장되었다. 세포는 프로테오믹스 분석을 위해 수확되었고, 나머지 세포 배양물은 다른 곳에 기재된 바이오어쎄이를 이용하여 비오틴 생산이 측정되기 전에, 총 24시간 동안 인큐베이션이 유지되었다.
그래프 (도 11)로부터, 측정된 비오틴 단백질 수준은 비오틴 생산과 밀접한 관련이 있다 (R2　값0.96). 선형 상관관계는 향상된 BioB 발현을 촉진시키는 것이 IscR 돌연변이 세포 공장에서 비오틴 생산을 향상시키는 핵심임을 나타낸다. 프로테오믹스 데이터의 ANOVA 분석은 추가적인 29 단백질에서 발현의 현저한 증가 (95% 신뢰 구간, p-값 0.00166)를 나타냈다. 이들 중에서 isc-오페론 (IscA 및 IscS) 및 suf-오페론 (SufB 및 SufS)의 구성원이 있다.
2.7 iscR 녹아웃 돌연변이에서 비오틴 생산은 향상되지 않는다
iscR 유전자의 번역 녹아웃 (translational knockout)은 iscR에서의 22번 위치 상에서 글루탐산을 코딩하는 코돈 (E, GAA)을 종결 코돈 (*, TGA)으로 변환함으로써, MAGE에 의해, BW25113 △bioB 균주 내로 도입되었다. 상기 코돈의 성공적인 변환은 상기 영역의 PCR 증폭 및 이후의 생거 시퀀싱 (Sanger sequencing)에 의해 입증되었따. 야생형 iscR, iscR 녹아웃 (E22*), 및 돌연변이 iscR (C92Y)를 코딩하는 유전자를 갖는 균주는 IPTG-유도성 bioB 플라스미드 pBS412로 형질전환되었고, 상기 기재된 바와 같은 3개의 상이한 IPTG 유도 수준 (0, 0.01, 및 0.1 mM)에서 0.1 g/l DTB 및 50 ㎍/l 카나마이신으로 보충된 mMOPS에서 성장된 생물학적 복제물 (n=3)에서 비오틴 생산에 대해 시험하였다.
IPTG 유도에 의해 bioB 발현을 유도한 경우 iscR 녹아웃 (iscR KO) 및 야생형 iscR (iscR WT) 사이에 비오틴 생산에 있어 유의적인 차이는 관찰되지 않았다. 이는 iscR을 녹아웃하는 것이 비오틴 생산을 개선시키지 않는다는 증거를 제공한다. iscR WT 및 iscR KO 균주 모두와 비교하여, IscR C92Y 치환을 코딩하는 돌연변이 iscR에서 비오틴 생산에 있어 유의적인 개선이 다시 관찰되었다.
2.8 본 발명의 iscR 돌연변이 균주에서 드-노보 (De-novo) 비오틴 생산이 향상된다
bioA 유전자 및 전체 비오틴-오페론 (△bioB-△bioD)이 결실되고, iscR WT, iscR H107Y 돌연변이 또는 iscR C92Y 유전자를 포함하는, BW25113 대장균 균주는 천연 대장균 bioA 및 bioO 오퍼레이터 상에 싱글 포인트 돌연변이 (타입 9 돌연변이, Ifuku et al., 1993)를 갖는 비오틴-오페론을 항시적으로 과발현하는 테트라사이클린 내성 플라스미드로 형질전환되었다. 상기 기재된 바와 같이 (도 13), 0.1 g/l DTB를 첨가하거나 첨가하지 않고 10 ㎍/ml 테트라사이클린을 갖는 mMOPS (2 g 글루코스/l)에서의 생물학적 복제물 (n=4)에서 3개의 상이한 균주에 대하여 비오틴 생산이 평가되었다.
기질, DTB가 증식 배지에 첨가된 경우, 모든 3개의 균주에서 비오틴 역가의 현저한 증가가 관찰되었고, 이는 DTB를 비오틴으로 전환하는, bioB 효소 반응 자체가 더이상 이들 균주에서 비오틴 생산에 장애물 (bottleneck)이 아님을 나타낸다 (도 13). 또한, iscR WT 균주와 비교하여 iscR 돌연변이 균주 모두에서 글루코스로부터 비오틴의 드-노보 생산의 현저한 증가가 관찰되었다. 이들 결과를 고려하면, 본 발명의 모든 돌연변이 iscR 균주는 직접적인 전구체, DTB, 및 글루코스 모두로부터 향상된 비오틴 생산을 지지하는 것으로 추론될 수 있다.
실시예 2: 리포산 생산을 향상시킬 수 있는 유전자 변형 대장균 균주의 설계 및 특성화
실시예에서 사용된 하기의 에셰리키아 콜라이 균주는 하기에 나열된다.
균주
명칭 설명
BS1912 E.coli K-12 BW25113로부터 유래된 △lipdA
BS2114 iscR에서 H107Y 돌연변이를 포함하는 BS1912 파생체
실시예에서 사용된 하기의 플라스미드는 하기에 나열된다.
플라스미드
명칭 설명
pBS993 AceF [서열번호 119] 발현의 추가적인 항시성 발현을 갖는 T5 lacO 억제 프로모터 [서열번호 234]로부터의 Lipid A [서열번호 103] 과발현 플라스미드 (kanR, SC101)
pBS1037 AceF [서열번호 119] 발현의 낮은 RBS 강도 및 SC101 대신에 p15A 복제 기점을 갖는 pBS993 파생체
pBS451 항시적으로 발현되는 GFP [서열번호 287] (zero, p15A)
플라스미드 pBS1037 상에 클로닝된, LipA [서열번호 103]를 코딩하는 IIPTG-유도성 전이 유전자는, 실시예 1에 기재된 바와 같이, 천연 iscR 유전자를 포함하는 대장균 숙주 균주 또는 천연 iscR 유전자가 C92Y 또는 H107Y 치환을 갖는 IscR 단백질을 코딩하는 돌연변이 iscR 유전자로 치환된 대장균 숙주 균주 내로 도입되었다; 상개 2개의 균주는 bioB 또는 lipA의 녹-아웃을 더 포함한다. 상기 균주들은 0.1 mM 비오틴 (△bioB 균주를 위함), lipA 발현의 유도를 위한 IPTG 및 기질로서 0.6 g/l 옥탄산으로 보충된 mMOPS 배지 (섹션 1.3에 기재된 바와 같은)에서, 24시간 동안 37℃에서, 상층액에서의 자유(free) 리포산의 측정 전에, 배양되었다. △lipA 균주 (BS1912 및 BS2114, 표 3)의 경우 37℃에서 24시간의 성장이 뒤따랐다.
기재된 균주들의 배양된 세포에 의해 생산된, 리포산은 pBS451을 포함한 BS1912를 이용한 섹션 1.6에 기재된 것과 유사한 바이오어쎄이를 이용하여 상층액으로부터 측정되었다. 리포산의 정량화를 위한, 성장-기반의 바이오어쎄이는 리포산을 합성할 수 없는 영양요구성 대장균 단일 △lipA 돌연변이 균주를 이용하여 수행되었다 (Herbert and Guest, 1975) (pBS451을 포함하는 BS1912). 상기 상층액에서의 자유 리포산 농도는 리포산의 단독 공급원으로서 생산 균주로부터 회수된 리포산으로 보충되고, 탄소원으로서 50 nN na-숙시네이트를 갖는 최소 배지에서의 리포산 영양요구성 균주의 성장을 측정함으로써 결정되었다. 공지된 농도 범위의 리포산 표준으로 보충된 최소 배지 상에서 영양요구성 균주가 성장되는 경우와, 리포산 바이오어쎄이 검정 곡선은 병렬로 수행되었다.
상기 시험은 IscR 단백질의 돌연변이 형태 (C92Y 또는 H107 치환을 갖는 IscR 단백질)를 코딩하는 유전자를 포함하는 대장균 균주에서의 LipA 유전자의 과-발현은, IscR 단백질의 천연 형태를 코딩하는 유전자를 포함하는 모체 대장균 균주에서의 LipA 유전자의 과발현과 비교하여, 더 안정적인 생산 및 리포산 역가의 80% 증가를 나타냄을 입증한다 (도 14). 따라서 iscR WT 균주 (pBS993을 갖는 BS1011)의 생산 역가에서의 표준 편차는 2.73인 반면에 iscR C92Y (pBS993을 갖는 BS1375)의 경우 1.42이고 iscR H107Y (pBS993을 갖는 BS1353)의 경우 0.11로 낮다 (균주 표준에 대한 표 1 참조). 개별적인 균주의 평균 생산 역가에 기초하여, 리포산 생산은 WT 균주와 비교하여 돌연변이 균주에서 1.79-배 개선되었다 (도 14 참조).
WT iscR 균주 (삼각형, pBS1037을 포함하는 BS1912) 및 iscR 돌연변이 균주 (사각형, pBS1047을 포함하는 BS2114) 둘다의 경우에서, LipA의 과발현은 IPTG에 의한 LipA의 증가된 유도에 반응하여 성장률이 감소하는 명확한 경향을 나타내었다 (더 어두운 음영을 넣은 회색, 도 15). 그러나, WT iscR 균주의 성장률은 0.01 mM 내지 0.03 mM 내지의 시험된 모든 IPTG 유도 수준에서 돌연변이 iscR 균주와 비교하여 보다 극심하게 감소되었다 (도 15 참조).
실시예 3 티아민 생산을 향상시킬 수 있는 유전자 변형 대장균 균주의 설계 및 특성화
실시예에서 사용된 하기의 에셰리키아 콜라이 균주는 하기에 나열된다.
균주
명칭 설명
BS750 코딩된 TMP 키나아제에서 G133D 치환을 일으키는 천연 thiL 유전자: 코돈 133에서 GGT로부터 GAC로의 점 돌연변이를 포함하는 BS1013 파생체, BW25113 △thiP
BS2019 iscR에 C92Y 돌연변이를 포함하는 BS750 파생체
BS2020 iscR에 H107Y 돌연변이를 포함하는 BS750 파생체
실시예에서 사용된 하기의 플라스미드는 하기에 나열된다.
명칭 설명
pBS140 thiC 오페론 (apFAB46 프로모터 [서열번호 147] 및apFAB377 종결자 [서열번호 153]에 기능적으로 연결됨) 및 thiM 오페론 (apFAB71 프로모터 [서열번호 149] 및 apFAB378 종결자 [서열번호 152]에 기능적으로 연결됨)의 조합으로 구성된; 대장균 티아민 경로 유전자 thiCEFSGHMD를 포함하는 벡터
pBS100 pBS140의 구성에 사용되기 위한 빈 벡터
pBS93 pFAB70 프로모터 [서열번호 148] 및 ap FAB381 종결자 [서열번호 154]에 기능적으로 연결된 대장균에서의 발현에 최적화된 애기장대 (Arabidopsis thaliana) AT5G32470.1 포스파타아제 코돈을 코딩하는 합성 유전자 (synthetic gene)를 포함하는 벡터
pBS209 대장균으로부터 추가적인 thiC를 갖는 pBS140에 기반한 플라스미드
실시예 1에 기재된 바와 같이, 플라스미드 pBS140에 클로닝된 티아민 경로 유전자 thiCEFSGHMD는 천연 iscR 유전자를 포함하는 대장균 숙주 균주 (BS750) (표준 균주)뿐만 아니라 상기 천연 iscR 유전자가 C92Y 또는 H107Y 치환을 각각 갖는 IscR을 코딩하는 돌연변이 iscR 유전자에 의해 치환된 이 표준 균주 (BS2019 및 BS2020)의 파생체 내로 도입되었다. 상기 균주들은 딥 배양 플레이트의 개별적인 웰에서 24시간 동안 37℃에서 mMOPS 배지 (섹션 1.3에 기재된 바와 같음)에서 배양되었다.
기재된 균주들의 배양된 세포들에 의해 생산된 세포 외 및 세포 내 티아민, TMP 및 TPP는 다음과 같이 회수되고 추출되었다: 각 배양물의 0.4 mL는 배양 플레이트에서 5분 동안 4000 x g에서 원심분리에 의해 4℃에서 수확되었다. 나머지 모든 단계는 얼음 상에서 수행되었다. 세포 외 TPP, TMP 및 티아민의 분석을 위해 상층액의 40 ㎍은 부드럽게 제거되었다. 남은 상층액을 따라낸 후, 배양 플레이트는 잔류 배지를 제거하기 위해 뒤집은 후 볼텍싱되었다 (voltexed). 100μL 아이스-콜드 HPLC 그레이드 메탄올 (HPLC grade methanol)을 배양 플레이트의 각 웰에 첨가되었고; 세포는 다시 볼텍싱되었다. 얼음 상에서 최소 20분 동안 인큐베이션 후, 세포 잔해물은 5분 동안 4000 x g에서의 원심분리에 의해 펠렛화되었다. 상층액은 추가적인 분석을 위한 세포 내 추출물로서 사용되었다.
형광 검출기를 이용하여 TPP, TMP 및 티아민을 검출하기 위해, 각 배양물에 의해 생산된 티아민 화합물은 강한 형광성인 티오크롬으로 유도체화되었다 (derivatized). 모든 단계는 실온에서 수행되었다. 세포 외 및 세포 내 추출물의 40 ㎕ 부피는 4M 포타슘 아세테이트의 80 ㎕에 첨가되었고, 피펫팅 (pipetting)에 의해 혼합되었다. 새로 제조된 7M NaOH에서의 3.8 mM 포타슘 페리사이아나이드 (potassium ferricyanide) 40 ㎕가 첨가되고 혼합되었다. 새로 제조된 포화된 KH2P04에서의 0.06% H202 40 ㎕의 첨가에 의해 상기 반응은 억제되었다 (quenched). 상기 추출물은 6M HCl 47 ㎕의 첨가에 의해 중화되었고 이후 HPLC 또는 멀티스칸을 이용한 직접적인 형광 측정에 의해 분석되었다. 유도체화된 모든 화합물은 상기 분석된 추출물과 병렬하여 티오크롬으로 유도체화된 새로 제조된 TPP, TMP 및 티아민 표준의 형광 표준 곡선을 이용하여 정량화되었다.
상기 시험은 IscR 단백질의 돌연변이 형태 (C92Y 또는 H107Y 치환을 갖는 IscR 단백질)를 코딩하는 유전자를 포함하는 숙주 대장균 균주 (BS2019 및 BS202)에서의 TMP 포스파타제 유전자 (At5g32470)와 조합하여 thiC 유전자 및 thiH 유전자를 포함한 티아민 경로 유전자의 과-발현은, IscR 단백질의 천연 형태를 코딩하는 유전자를 포함하는 숙주 대장균 균주 (BS750)에서의 과-발현과 비교하여, 티아민, TMP 및 TPP, 특히 티아민의 생합성을 향상시킴을 입증한다.
보다 구체적으로, 상기 시험은 WT iscR을 갖는 균주 (BS750)와 pBS140을 사용하는 경우 iscR 돌연변이를 코딩하는 균주 (BS2020, H107Y 또는 BS2019, C92Y) 사이에 티아민 (티아민, TMP 및 TPP)의 OD-표준화된 세포 외 생산에 있어 1.43 배의 증가를 나타내었다 (도 16).
실시예 4 비오틴을 생산할 수 있는 유전자 변형 대장균 균주의 생산성을 증가시키기 위한 플라보독신/페레독신 환원 효소 (Fpr)의 과발현 및 플라보독신 (FldA) 환원 시스템
1 방법
1.1 : 실시예에서 사용된 하기의 에셰리키아 콜라이 균주는 하기에 나열된다.
균주
명칭 설명
BS1013 다음의 유전자형을 갖는 E. coli K-12 BW25113 모체 균주: rrnB3 △lacZ4787 hsdR514 △(araBAD)567 △(rhaBAD)568 rph-1
BS1011 E. coli K-12 BW25113로부터 유래된 △bioB (JW0758-1)
BS1353 iscR에 H107Y 돌연변이를 포함하는 BS1011 파생체
BS1615 △bioAFCD의 추가적인 결실을 갖는 BS1011 파생체
BS1937 IPTG-유도성 BioB 발현을 제공하는 pBS679 플라스미드를 포함하는 BS1615 파생체
BS2185 IPTG-유도성 BioB 발현을 제공하는 pBS679 플라스미드 및 FldA-Fpr 항시적 발현을 제공하는 pBS1112를 포함하는 BS1615 파생체
BS2707 IPTG-유도성 BioB 발현을 제공하는 pBS679 플라스미드 및 GFP 항시적 발현을 제공하는 pBS1054를 포함하는 BS1615 파생체
실시예에서 사용된 하기의 플라스미드는 하기에 나열된다.
플라스미드
명칭 설명
pBS679 T5 lacO 억제 프로모터 [서열번호 25]로부터의 BioB [서열번호 22] 과발현 플라스미드 (ampR, pSC101) 및
pBS1054 apFAB378 종결자 [서열번호 292]를 갖는 apFAB309 항시적 프로모터 [서열번호 291]로부터의 GFP [서열번호 276] 과발현 플라스미드 (kanR, pBR322)
pBS1112 apFAB306 항시적 프로모터 (apFAB306-FldA-Fpr 유전자-apFAB378 종결자 [서열번호 288])로부터의 FldA-Fpr 과발현 플라스미드 (kanR, pBR322)
BioB를 코딩하는 IPTG-유도성 전이 유전자는 플라스미드 pBS679 상에 클로닝되었고; GFP를 코딩하는 항시적으로-조절되는 전이 유전자는 플라스미드 pBS1054에 클로닝되었고; FldA-렉을 코딩하는 합성 오페론 (synthetic operon)를 포함하는 항시적으로-조절되는 전이 유전자는 플라스미드 pBS1112 상에 클로닝되었다. pBS679는 실시예 1에 기재된 바와 같이, 천연 iscR 유전자가 H107Y 치환을 갖는 IscR 단백질을 코딩하는 돌연변이 iscR 유전자에 의해 치환되고, BS1937 균주를 생성하는 bioAFCD 유전자의 녹-아웃을 더 포함하는 대장균 숙주 균주 (BS1615) 내로 도입되었다. 상기 BS1937 균주는 BS2707 (대조군 균주) 및 BS2185 균주 각각을 생성하도록 플라스미드 pBS1054 또는 pBS1112로 더 형질전환되었다.
상기 균주들은 적합한 항생제, BioB-매개 촉매를 위한 기질로서 0.1 g/l을 가지고, BioB 유전자의 발현을 유도하기 위한 0, 0.01, 0.025, 0.05, 0.075 또는 0.1 mM IPTG로 보충된 mMOPS 배지 (실시예 1.3에 기재된 바와 같음)에서 배양되었다. 상기 세포들은 딥 웰 배양 플레이트의 개별적인 웰에서 24시간 동안 37℃에서 인큐베이션되었다. 최종 OD가 예측되었고, 상층액은 원심분리에 의해 수확되었고, 실시예 1.6에 의해 기재된 바와 같이 비오틴 바이오어쎄이에 의해 상층액으로부터 비오틴이 정량화되었다.
균주 BS1937에 대한 도 12 및 도 17에 나타낸 바와 같이, 유전적으로 변형된 내인성 iscR 유전자를 포함하는 대장균 세포에서 BioB 유전자 발현이 IPTG 농도의 증가에 의해 유도된 경우, 상기 세포는 상응하는 비오틴 생산에 있어 점진적인 증가를 나타낸다. 이들 유전자 변형 세포에서 비오틴 생산은 이의 모체 균주 BS1937 및 FldA-Fpr 대신에 GFP를 코딩하는 전이유전자를 발현하는 대조군 균주와 비교하여, FldA-Fpr을 코딩하는 전이 유전자의 공동-발현 (BS2185 균주)에 의해 더 향상된다.
IscR 단백질의 돌연변이 형태 (H107Y 치환을 갖는 IscR 단백질)를 코딩하는 유전자, BioB (pBS679) 및 FldA-Fpr 유전자 (pBS1112)의 과발현을 위한 플라스미드를 포함하는 BS2185 균주의 비오틴 생산은 대조군 균주 BS1937 (FldA-Fpr 유전자의 과발현 없음)과 비교하여 2.12-배 향상된다 (도 18).
참고문헌
Datsenko KA, Wanner BL. (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A. ;97(12) : 6640-5.
Fleischhacker, A. S. et al. (2012) Characterization of the [2Fe-2S] cluster of Escherichia coli transcription factor IscR. Biochemistry 51 : 4453-4462.
Giel, J. L., Rodionov, D., Liu, M., Blattner, F. R. & Kiley, P. J. (2006) IscR-dependent gene expression links iron-sulphur cluster assembly to the control of 02-regulated genes in Escherichia coli. Mol. Microbiol. 60, 1058-1075.
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SEQUENCE LISTING <110> Biosyntia ApS <120> Cell factory having improved iron-sulfur cluster delivery <130> P2296PC00 <150> EP17181503.8 <151> 2017-07-14 <160> 292 <170> PatentIn version 3.5 <210> 1 <211> 489 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(489) <223> iscR WT gene encoding Iron Sulfur Cluster Regulator protein (IscR) <400> 1 atg aga ctg aca tct aaa ggg cgc tat gcc gtg acc gca atg ctt gac 48 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 gtt gcg ctc aac tct gaa gcg ggc ccg gta ccg ttg gct gat att tcc 96 Val Ala Leu Asn Ser Glu Ala Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 gaa cgt cag gga att tcc ctt tct tat ctg gaa caa ctg ttt tcc cgt 144 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 ctg cgt aaa aat ggt ctg gtt tcc agc gta cgt gga cca ggc ggt ggt 192 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 tat ctg tta ggc aaa gat gcc agc agc atc gcc gtt ggc gaa gta att 240 Tyr Leu Leu Gly Lys Asp Ala Ser Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 agc gcc gtt gac gaa tct gta gat gcc acc cgt tgt cag ggt aaa ggc 288 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Gly 85 90 95 ggc tgc cag ggc ggc gat aaa tgc ctg acc cac gcg ctg tgg cgt gat 336 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 ttg agc gac cgt ctc acc ggt ttt ctc aac aac att act tta ggc gaa 384 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 ctg gtt aat aac cag gaa gtg ctg gat gtg tct ggt cgt cag cat act 432 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Thr 130 135 140 cac gac gcg cca cgc acc cgc aca caa gac gcg atc gac gtt aag tta 480 His Asp Ala Pro Arg Thr Arg Thr Gln Asp Ala Ile Asp Val Lys Leu 145 150 155 160 cgc gct taa 489 Arg Ala <210> 2 <211> 162 <212> PRT <213> Escherichia coli <400> 2 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 Val Ala Leu Asn Ser Glu Ala Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 Tyr Leu Leu Gly Lys Asp Ala Ser Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Gly 85 90 95 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Thr 130 135 140 His Asp Ala Pro Arg Thr Arg Thr Gln Asp Ala Ile Asp Val Lys Leu 145 150 155 160 Arg Ala <210> 3 <211> 486 <212> DNA <213> Shigella sonnei <220> <221> CDS <222> (1)..(486) <223> iscR gene encoding Iron Sulfur Cluster Regulator <400> 3 atg cgc ctg acc agc aaa ggc cgc tat gcg gtg acc gcg atg ctg gat 48 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 gtg gcg ctg aac agc gaa gcg ggc ccg gtg ccg ctg gcg gat att agc 96 Val Ala Leu Asn Ser Glu Ala Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 gaa cgc cag ggc att agc ctg agc tat ctg gaa cag ctg ttt agc cgc 144 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 ctg cgc aaa aac ggc ctg gtg agc agc gtg cgc ggc ccg ggc ggc ggc 192 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 tat ctg ctg ggc aaa gat gcg agc agc att gcg gtg ggc gaa gtg att 240 Tyr Leu Leu Gly Lys Asp Ala Ser Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 agc gcg gtg gat gaa agc gtg gat gcg acc cgc tgc cag ggc aaa ggc 288 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Gly 85 90 95 ggc tgc cag ggc ggc gat aaa tgc ctg acc cat gcg ctg tgg cgc gat 336 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 ctg agc gat cgc ctg acc ggc ttt ctg aac aac att acc ctg ggc gaa 384 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 ctg gtg aac aac cag gaa gtg ctg gat gtg agc ggc cgc cag cat acc 432 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Thr 130 135 140 cat gat gcg ccg cgc acc cgc att ctg gat gcg att gat gtg aaa ctg 480 His Asp Ala Pro Arg Thr Arg Ile Leu Asp Ala Ile Asp Val Lys Leu 145 150 155 160 cgc gcg 486 Arg Ala <210> 4 <211> 162 <212> PRT <213> Shigella sonnei <400> 4 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 Val Ala Leu Asn Ser Glu Ala Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 Tyr Leu Leu Gly Lys Asp Ala Ser Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Gly 85 90 95 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Thr 130 135 140 His Asp Ala Pro Arg Thr Arg Ile Leu Asp Ala Ile Asp Val Lys Leu 145 150 155 160 Arg Ala <210> 5 <211> 489 <212> DNA <213> Citrobacter pasteurii <220> <221> CDS <222> (1)..(489) <223> iscR gene encoding Iron Sulfur Cluster Regulator <400> 5 atg cgc ctg acc agc aaa ggc cgc tat gcg gtg acc gcg atg ctg gat 48 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 gtg gcg ctg aac agc gaa acc ggc ccg gtg ccg ctg gcg gat att agc 96 Val Ala Leu Asn Ser Glu Thr Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 gaa cgc cag ggc att agc ctg agc tat ctg gaa cag ctg ttt agc cgc 144 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 ctg cgc aaa aac ggc ctg gtg agc agc gtg cgc ggc ccg ggc ggc ggc 192 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 tat ctg ctg ggc aaa gat gcg agc agc att gcg gtg ggc gaa gtg att 240 Tyr Leu Leu Gly Lys Asp Ala Ser Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 agc gcg gtg gat gaa agc gtg gat gcg acc cgc tgc cag ggc aaa ggc 288 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Gly 85 90 95 ggc tgc cag ggc ggc gat aaa tgc ctg acc cat gcg ctg tgg cgc gat 336 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 ctg agc gat cgc ctg acc ggc ttt ctg aac aac att acc ctg ggc gaa 384 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 ctg gtg aac aac cag gaa gtg ctg gat gtg agc ggc cgc cag cat acc 432 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Thr 130 135 140 cat gat gcg ccg cgc acc aac cgc gcg cag gat gcg att gat gtg aaa 480 His Asp Ala Pro Arg Thr Asn Arg Ala Gln Asp Ala Ile Asp Val Lys 145 150 155 160 ctg cgc gcg 489 Leu Arg Ala <210> 6 <211> 163 <212> PRT <213> Citrobacter pasteurii <400> 6 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 Val Ala Leu Asn Ser Glu Thr Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 Tyr Leu Leu Gly Lys Asp Ala Ser Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Gly 85 90 95 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Thr 130 135 140 His Asp Ala Pro Arg Thr Asn Arg Ala Gln Asp Ala Ile Asp Val Lys 145 150 155 160 Leu Arg Ala <210> 7 <211> 489 <212> DNA <213> Enterobacter timonensis <220> <221> CDS <222> (1)..(489) <223> iscR gene encoding Iron Sulfur Cluster Regulator <400> 7 atg cgc ctg acc agc aaa ggc cgc tat gcg gtg acc gcg atg ctg gat 48 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 gtg gcg ctg aac agc gaa gcg ggc ccg gtg ccg ctg gcg gat att agc 96 Val Ala Leu Asn Ser Glu Ala Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 gaa cgc cag ggc att agc ctg agc tat ctg gaa cag ctg ttt agc cgc 144 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 ctg cgc aaa aac ggc ctg gtg agc agc gtg cgc ggc ccg ggc ggc ggc 192 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 tat ctg ctg ggc aaa gat gcg ggc agc att gcg gtg ggc gaa gtg att 240 Tyr Leu Leu Gly Lys Asp Ala Gly Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 agc gcg gtg gat gaa agc gtg gat gcg acc cgc tgc cag ggc aaa ggc 288 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Gly 85 90 95 ggc tgc cag ggc ggc gat aaa tgc ctg acc cat gcg ctg tgg cgc gat 336 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 ctg agc gat cgc ctg acc ggc ttt ctg aac aac att acc ctg ggc gaa 384 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 ctg gtg aac aac cag gaa gtg ctg gat gtg agc ggc cgc cag cat agc 432 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Ser 130 135 140 cat gat agc cag cgc aac acc cgc gcg cag gat gcg att gat gtg aaa 480 His Asp Ser Gln Arg Asn Thr Arg Ala Gln Asp Ala Ile Asp Val Lys 145 150 155 160 ctg cgc gcg 489 Leu Arg Ala <210> 8 <211> 163 <212> PRT <213> Enterobacter timonensis <400> 8 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 Val Ala Leu Asn Ser Glu Ala Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 Tyr Leu Leu Gly Lys Asp Ala Gly Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Gly 85 90 95 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Ser 130 135 140 His Asp Ser Gln Arg Asn Thr Arg Ala Gln Asp Ala Ile Asp Val Lys 145 150 155 160 Leu Arg Ala <210> 9 <211> 489 <212> DNA <213> Pluralibacter gergoviae <220> <221> CDS <222> (1)..(489) <223> iscR gene encoding Iron Sulfur Cluster Regulator <400> 9 atg cgc ctg acc agc aaa ggc cgc tat gcg gtg acc gcg atg ctg gat 48 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 gtg gcg ctg aac agc gaa agc ggc ccg gtg ccg ctg gcg gat att agc 96 Val Ala Leu Asn Ser Glu Ser Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 gaa cgc cag ggc att agc ctg agc tat ctg gaa cag ctg ttt agc cgc 144 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 ctg cgc aaa aac ggc ctg gtg agc agc gtg cgc ggc ccg ggc ggc ggc 192 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 tat ctg ctg ggc aaa gat gcg ggc agc att gcg gtg ggc gaa gtg att 240 Tyr Leu Leu Gly Lys Asp Ala Gly Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 agc gcg gtg gat gaa agc gtg gat gcg acc cgc tgc cag ggc aaa gcg 288 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Ala 85 90 95 ggc tgc cag ggc ggc gat aaa tgc ctg acc cat gcg ctg tgg cgc gat 336 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 ctg agc gat cgc ctg acc ggc ttt ctg aac aac att acc ctg ggc gaa 384 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 ctg gtg aac aac cag gaa gtg ctg gat gtg agc gat cgc cag cat att 432 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Asp Arg Gln His Ile 130 135 140 cat gaa acc cag cgc agc acc cgc agc cag gat gcg att gat gtg aaa 480 His Glu Thr Gln Arg Ser Thr Arg Ser Gln Asp Ala Ile Asp Val Lys 145 150 155 160 ctg cgc gcg 489 Leu Arg Ala <210> 10 <211> 163 <212> PRT <213> Pluralibacter gergoviae <400> 10 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 Val Ala Leu Asn Ser Glu Ser Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 Tyr Leu Leu Gly Lys Asp Ala Gly Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Ala 85 90 95 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Asp Arg Gln His Ile 130 135 140 His Glu Thr Gln Arg Ser Thr Arg Ser Gln Asp Ala Ile Asp Val Lys 145 150 155 160 Leu Arg Ala <210> 11 <211> 486 <212> DNA <213> Buttiauxella <220> <221> CDS <222> (1)..(486) <223> iscR gene encoding Iron Sulfur Cluster Regulator <400> 11 atg cgc ctg acc agc aaa ggc cgc tat gcg gtg acc gcg atg ctg gat 48 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 gtg gcg ctg aac agc gaa agc ggc ccg gtg ccg ctg gcg gat att agc 96 Val Ala Leu Asn Ser Glu Ser Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 gaa cgc cag ggc att agc ctg agc tat ctg gaa cag ctg ttt agc cgc 144 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 ctg cgc aaa aac ggc ctg gtg gcg agc gtg cgc ggc ccg ggc ggc ggc 192 Leu Arg Lys Asn Gly Leu Val Ala Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 tat ctg ctg ggc aaa gaa gcg agc gcg att gcg gtg ggc gaa gtg att 240 Tyr Leu Leu Gly Lys Glu Ala Ser Ala Ile Ala Val Gly Glu Val Ile 65 70 75 80 agc gcg gtg gat gaa agc gtg gat gcg acc cgc tgc gcg ggc aaa ggc 288 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Ala Gly Lys Gly 85 90 95 ggc tgc cag ggc ggc gat aaa tgc ctg acc cat gcg ctg tgg cgc gat 336 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 ctg agc gat cgc ctg acc ggc ttt ctg aac aac att acc ctg ggc gaa 384 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 ctg gtg aac aac cag gaa gtg ctg gat gtg agc ggc cgc cag cat aac 432 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Asn 130 135 140 gaa aac cat cgc agc acc cgc agc cag gat gcg att gat gtg aaa ctg 480 Glu Asn His Arg Ser Thr Arg Ser Gln Asp Ala Ile Asp Val Lys Leu 145 150 155 160 cgc gcg 486 Arg Ala <210> 12 <211> 162 <212> PRT <213> Buttiauxella <400> 12 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 Val Ala Leu Asn Ser Glu Ser Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 Leu Arg Lys Asn Gly Leu Val Ala Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 Tyr Leu Leu Gly Lys Glu Ala Ser Ala Ile Ala Val Gly Glu Val Ile 65 70 75 80 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Ala Gly Lys Gly 85 90 95 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Asn 130 135 140 Glu Asn His Arg Ser Thr Arg Ser Gln Asp Ala Ile Asp Val Lys Leu 145 150 155 160 Arg Ala <210> 13 <211> 489 <212> DNA <213> Kosakonia sacchari <220> <221> CDS <222> (1)..(489) <223> iscR gene encoding Iron Sulfur Cluster Regulator <400> 13 atg cgc ctg acc agc aaa ggc cgc tat gcg gtg acc gcg atg ctg gat 48 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 gtg gcg ctg aac agc gaa gcg ggc ccg gtg ccg ctg gcg gat att agc 96 Val Ala Leu Asn Ser Glu Ala Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 gaa cgc cag ggc att agc ctg agc tat ctg gaa cag ctg ttt agc cgc 144 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 ctg cgc aaa aac ggc ctg gtg gcg agc gtg cgc ggc ccg ggc ggc ggc 192 Leu Arg Lys Asn Gly Leu Val Ala Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 tat ctg ctg ggc aaa gat gcg aac acc att gcg gtg ggc gaa gtg att 240 Tyr Leu Leu Gly Lys Asp Ala Asn Thr Ile Ala Val Gly Glu Val Ile 65 70 75 80 agc gcg gtg gat gaa agc gtg gat gcg acc cgc tgc cag ggc aaa agc 288 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Ser 85 90 95 ggc tgc cag ggc ggc gat aaa tgc ctg acc cat gcg ctg tgg cgc gat 336 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 ctg agc gat cgc ctg acc ggc ttt ctg aac aac att acc ctg ggc gaa 384 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 ctg gtg aac aac cag gaa att ctg gat gtg agc gat cgc cag cat aac 432 Leu Val Asn Asn Gln Glu Ile Leu Asp Val Ser Asp Arg Gln His Asn 130 135 140 aac gaa agc cat cgc aac acc cgc ggc cag gat gcg att gat gtg aaa 480 Asn Glu Ser His Arg Asn Thr Arg Gly Gln Asp Ala Ile Asp Val Lys 145 150 155 160 ctg cgc gcg 489 Leu Arg Ala <210> 14 <211> 163 <212> PRT <213> Kosakonia sacchari <400> 14 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 Val Ala Leu Asn Ser Glu Ala Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 Leu Arg Lys Asn Gly Leu Val Ala Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 Tyr Leu Leu Gly Lys Asp Ala Asn Thr Ile Ala Val Gly Glu Val Ile 65 70 75 80 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Ser 85 90 95 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 Leu Val Asn Asn Gln Glu Ile Leu Asp Val Ser Asp Arg Gln His Asn 130 135 140 Asn Glu Ser His Arg Asn Thr Arg Gly Gln Asp Ala Ile Asp Val Lys 145 150 155 160 Leu Arg Ala <210> 15 <211> 489 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(489) <223> iscR mutant gene encoding Iron Sulfur Cluster Regulator protein (IscR) with L15F substitution <400> 15 atg aga ctg aca tct aaa ggg cgc tat gcc gtg acc gca atg ttt gac 48 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Phe Asp 1 5 10 15 gtt gcg ctc aac tct gaa gcg ggc ccg gta ccg ttg gct gat att tcc 96 Val Ala Leu Asn Ser Glu Ala Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 gaa cgt cag gga att tcc ctt tct tat ctg gaa caa ctg ttt tcc cgt 144 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 ctg cgt aaa aat ggt ctg gtt tcc agc gta cgt gga cca ggc ggt ggt 192 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 tat ctg tta ggc aaa gat gcc agc agc atc gcc gtt ggc gaa gta att 240 Tyr Leu Leu Gly Lys Asp Ala Ser Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 agc gcc gtt gac gaa tct gta gat gcc acc cgt tgt cag ggt aaa ggc 288 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Gly 85 90 95 ggc tgc cag ggc ggc gat aaa tgc ctg acc cac gcg ctg tgg cgt gat 336 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 ttg agc gac cgt ctc acc ggt ttt ctc aac aac att act tta ggc gaa 384 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 ctg gtt aat aac cag gaa gtg ctg gat gtg tct ggt cgt cag cat act 432 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Thr 130 135 140 cac gac gcg cca cgc acc cgc aca caa gac gcg atc gac gtt aag tta 480 His Asp Ala Pro Arg Thr Arg Thr Gln Asp Ala Ile Asp Val Lys Leu 145 150 155 160 cgc gct taa 489 Arg Ala <210> 16 <211> 162 <212> PRT <213> Escherichia coli <400> 16 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Phe Asp 1 5 10 15 Val Ala Leu Asn Ser Glu Ala Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 Tyr Leu Leu Gly Lys Asp Ala Ser Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Gly 85 90 95 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Thr 130 135 140 His Asp Ala Pro Arg Thr Arg Thr Gln Asp Ala Ile Asp Val Lys Leu 145 150 155 160 Arg Ala <210> 17 <211> 489 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(489) <223> iscR mutant gene encoding Iron Sulfur Cluster Regulator protein (IscR) with C92Y substitution <400> 17 atg aga ctg aca tct aaa ggg cgc tat gcc gtg acc gca atg ctt gac 48 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 gtt gcg ctc aac tct gaa gcg ggc ccg gta ccg ttg gct gat att tcc 96 Val Ala Leu Asn Ser Glu Ala Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 gaa cgt cag gga att tcc ctt tct tat ctg gaa caa ctg ttt tcc cgt 144 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 ctg cgt aaa aat ggt ctg gtt tcc agc gta cgt gga cca ggc ggt ggt 192 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 tat ctg tta ggc aaa gat gcc agc agc atc gcc gtt ggc gaa gta att 240 Tyr Leu Leu Gly Lys Asp Ala Ser Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 agc gcc gtt gac gaa tct gta gat gcc acc cgt tat cag ggt aaa ggc 288 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Tyr Gln Gly Lys Gly 85 90 95 ggc tgc cag ggc ggc gat aaa tgc ctg acc cac gcg ctg tgg cgt gat 336 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 ttg agc gac cgt ctc acc ggt ttt ctc aac aac att act tta ggc gaa 384 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 ctg gtt aat aac cag gaa gtg ctg gat gtg tct ggt cgt cag cat act 432 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Thr 130 135 140 cac gac gcg cca cgc acc cgc aca caa gac gcg atc gac gtt aag tta 480 His Asp Ala Pro Arg Thr Arg Thr Gln Asp Ala Ile Asp Val Lys Leu 145 150 155 160 cgc gct taa 489 Arg Ala <210> 18 <211> 162 <212> PRT <213> Escherichia coli <400> 18 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 Val Ala Leu Asn Ser Glu Ala Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 Tyr Leu Leu Gly Lys Asp Ala Ser Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Tyr Gln Gly Lys Gly 85 90 95 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr His Ala Leu Trp Arg Asp 100 105 110 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Thr 130 135 140 His Asp Ala Pro Arg Thr Arg Thr Gln Asp Ala Ile Asp Val Lys Leu 145 150 155 160 Arg Ala <210> 19 <211> 489 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(489) <223> iscR mutant gene encoding Iron Sulfur Cluster Regulator protein (IscR) with H107Y substitution <400> 19 atg aga ctg aca tct aaa ggg cgc tat gcc gtg acc gca atg ctt gac 48 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 gtt gcg ctc aac tct gaa gcg ggc ccg gta ccg ttg gct gat att tcc 96 Val Ala Leu Asn Ser Glu Ala Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 gaa cgt cag gga att tcc ctt tct tat ctg gaa caa ctg ttt tcc cgt 144 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 ctg cgt aaa aat ggt ctg gtt tcc agc gta cgt gga cca ggc ggt ggt 192 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 tat ctg tta ggc aaa gat gcc agc agc atc gcc gtt ggc gaa gta att 240 Tyr Leu Leu Gly Lys Asp Ala Ser Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 agc gcc gtt gac gaa tct gta gat gcc acc cgt tgt cag ggt aaa ggc 288 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Gly 85 90 95 ggc tgc cag ggc ggc gat aaa tgc ctg acc tac gcg ctg tgg cgt gat 336 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr Tyr Ala Leu Trp Arg Asp 100 105 110 ttg agc gac cgt ctc acc ggt ttt ctc aac aac att act tta ggc gaa 384 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 ctg gtt aat aac cag gaa gtg ctg gat gtg tct ggt cgt cag cat act 432 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Thr 130 135 140 cac gac gcg cca cgc acc cgc aca caa gac gcg atc gac gtt aag tta 480 His Asp Ala Pro Arg Thr Arg Thr Gln Asp Ala Ile Asp Val Lys Leu 145 150 155 160 cgc gct taa 489 Arg Ala <210> 20 <211> 162 <212> PRT <213> Escherichia coli <400> 20 Met Arg Leu Thr Ser Lys Gly Arg Tyr Ala Val Thr Ala Met Leu Asp 1 5 10 15 Val Ala Leu Asn Ser Glu Ala Gly Pro Val Pro Leu Ala Asp Ile Ser 20 25 30 Glu Arg Gln Gly Ile Ser Leu Ser Tyr Leu Glu Gln Leu Phe Ser Arg 35 40 45 Leu Arg Lys Asn Gly Leu Val Ser Ser Val Arg Gly Pro Gly Gly Gly 50 55 60 Tyr Leu Leu Gly Lys Asp Ala Ser Ser Ile Ala Val Gly Glu Val Ile 65 70 75 80 Ser Ala Val Asp Glu Ser Val Asp Ala Thr Arg Cys Gln Gly Lys Gly 85 90 95 Gly Cys Gln Gly Gly Asp Lys Cys Leu Thr Tyr Ala Leu Trp Arg Asp 100 105 110 Leu Ser Asp Arg Leu Thr Gly Phe Leu Asn Asn Ile Thr Leu Gly Glu 115 120 125 Leu Val Asn Asn Gln Glu Val Leu Asp Val Ser Gly Arg Gln His Thr 130 135 140 His Asp Ala Pro Arg Thr Arg Thr Gln Asp Ala Ile Asp Val Lys Leu 145 150 155 160 Arg Ala <210> 21 <211> 1041 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(1041) <223> bioB gene encoding biotin synthase (EC 2.8.1.6) <400> 21 atg gct cac cgc cca cgc tgg aca ttg tcg caa gtc aca gaa tta ttt 48 Met Ala His Arg Pro Arg Trp Thr Leu Ser Gln Val Thr Glu Leu Phe 1 5 10 15 gaa aaa ccg ttg ctg gat ctg ctg ttt gaa gcg cag cag gtg cat cgc 96 Glu Lys Pro Leu Leu Asp Leu Leu Phe Glu Ala Gln Gln Val His Arg 20 25 30 cag cat ttc gat cct cgt cag gtg cag gtc agc acg ttg ctg tcg att 144 Gln His Phe Asp Pro Arg Gln Val Gln Val Ser Thr Leu Leu Ser Ile 35 40 45 aag acc gga gct tgt ccg gaa gat tgc aaa tac tgc ccg caa agc tcg 192 Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys Tyr Cys Pro Gln Ser Ser 50 55 60 cgc tac aaa acc ggg ctg gaa gcc gag cgg ttg atg gaa gtt gaa cag 240 Arg Tyr Lys Thr Gly Leu Glu Ala Glu Arg Leu Met Glu Val Glu Gln 65 70 75 80 gtg ctg gag tcg gcg cgc aaa gcg aaa gcg gca gga tcg acg cgc ttc 288 Val Leu Glu Ser Ala Arg Lys Ala Lys Ala Ala Gly Ser Thr Arg Phe 85 90 95 tgt atg ggc gcg gcg tgg aag aat ccc cac gaa cgc gat atg ccg tac 336 Cys Met Gly Ala Ala Trp Lys Asn Pro His Glu Arg Asp Met Pro Tyr 100 105 110 ctg gaa caa atg gtg cag ggg gta aaa gcg atg ggg ctg gag gcg tgt 384 Leu Glu Gln Met Val Gln Gly Val Lys Ala Met Gly Leu Glu Ala Cys 115 120 125 atg acg ctg ggc acg ttg agt gaa tct cag gcg cag cgc ctc gcg aac 432 Met Thr Leu Gly Thr Leu Ser Glu Ser Gln Ala Gln Arg Leu Ala Asn 130 135 140 gcc ggg ctg gat tac tac aac cac aac ctg gac acc tcg ccg gag ttt 480 Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu Asp Thr Ser Pro Glu Phe 145 150 155 160 tac ggc aat atc atc acc aca cgc act tat cag gaa cgc ctc gat acg 528 Tyr Gly Asn Ile Ile Thr Thr Arg Thr Tyr Gln Glu Arg Leu Asp Thr 165 170 175 ctg gaa aaa gtg cgc gat gcc ggg atc aaa gtc tgt tct ggc ggc att 576 Leu Glu Lys Val Arg Asp Ala Gly Ile Lys Val Cys Ser Gly Gly Ile 180 185 190 gtg ggc tta ggc gaa acg gta aaa gat cgc gcc gga tta ttg ctg caa 624 Val Gly Leu Gly Glu Thr Val Lys Asp Arg Ala Gly Leu Leu Leu Gln 195 200 205 ctg gca aac ctg ccg acg ccg ccg gaa agc gtg cca atc aac atg ctg 672 Leu Ala Asn Leu Pro Thr Pro Pro Glu Ser Val Pro Ile Asn Met Leu 210 215 220 gtg aag gtg aaa ggc acg ccg ctt gcc gat aac gat gat gtc gat gcc 720 Val Lys Val Lys Gly Thr Pro Leu Ala Asp Asn Asp Asp Val Asp Ala 225 230 235 240 ttt gat ttt att cgc acc att gcg gtc gcg cgg atc atg atg cca acc 768 Phe Asp Phe Ile Arg Thr Ile Ala Val Ala Arg Ile Met Met Pro Thr 245 250 255 tct tac gtg cgc ctt tct gcc gga cgc gag cag atg aac gaa cag act 816 Ser Tyr Val Arg Leu Ser Ala Gly Arg Glu Gln Met Asn Glu Gln Thr 260 265 270 cag gcg atg tgc ttt atg gca ggc gca aac tcg att ttc tac ggt tgc 864 Gln Ala Met Cys Phe Met Ala Gly Ala Asn Ser Ile Phe Tyr Gly Cys 275 280 285 aaa ctg ctg acc acg ccg aat ccg gaa gaa gat aaa gac ctg caa ctg 912 Lys Leu Leu Thr Thr Pro Asn Pro Glu Glu Asp Lys Asp Leu Gln Leu 290 295 300 ttc cgc aaa ctg ggg cta aat ccg cag caa act gcc gtg ctg gca ggg 960 Phe Arg Lys Leu Gly Leu Asn Pro Gln Gln Thr Ala Val Leu Ala Gly 305 310 315 320 gat aac gaa caa cag caa cgt ctt gaa cag gcg ctg atg acc ccg gac 1008 Asp Asn Glu Gln Gln Gln Arg Leu Glu Gln Ala Leu Met Thr Pro Asp 325 330 335 acc gac gaa tat tac aac gcg gca gca tta tga 1041 Thr Asp Glu Tyr Tyr Asn Ala Ala Ala Leu 340 345 <210> 22 <211> 346 <212> PRT <213> Escherichia coli <400> 22 Met Ala His Arg Pro Arg Trp Thr Leu Ser Gln Val Thr Glu Leu Phe 1 5 10 15 Glu Lys Pro Leu Leu Asp Leu Leu Phe Glu Ala Gln Gln Val His Arg 20 25 30 Gln His Phe Asp Pro Arg Gln Val Gln Val Ser Thr Leu Leu Ser Ile 35 40 45 Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys Tyr Cys Pro Gln Ser Ser 50 55 60 Arg Tyr Lys Thr Gly Leu Glu Ala Glu Arg Leu Met Glu Val Glu Gln 65 70 75 80 Val Leu Glu Ser Ala Arg Lys Ala Lys Ala Ala Gly Ser Thr Arg Phe 85 90 95 Cys Met Gly Ala Ala Trp Lys Asn Pro His Glu Arg Asp Met Pro Tyr 100 105 110 Leu Glu Gln Met Val Gln Gly Val Lys Ala Met Gly Leu Glu Ala Cys 115 120 125 Met Thr Leu Gly Thr Leu Ser Glu Ser Gln Ala Gln Arg Leu Ala Asn 130 135 140 Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu Asp Thr Ser Pro Glu Phe 145 150 155 160 Tyr Gly Asn Ile Ile Thr Thr Arg Thr Tyr Gln Glu Arg Leu Asp Thr 165 170 175 Leu Glu Lys Val Arg Asp Ala Gly Ile Lys Val Cys Ser Gly Gly Ile 180 185 190 Val Gly Leu Gly Glu Thr Val Lys Asp Arg Ala Gly Leu Leu Leu Gln 195 200 205 Leu Ala Asn Leu Pro Thr Pro Pro Glu Ser Val Pro Ile Asn Met Leu 210 215 220 Val Lys Val Lys Gly Thr Pro Leu Ala Asp Asn Asp Asp Val Asp Ala 225 230 235 240 Phe Asp Phe Ile Arg Thr Ile Ala Val Ala Arg Ile Met Met Pro Thr 245 250 255 Ser Tyr Val Arg Leu Ser Ala Gly Arg Glu Gln Met Asn Glu Gln Thr 260 265 270 Gln Ala Met Cys Phe Met Ala Gly Ala Asn Ser Ile Phe Tyr Gly Cys 275 280 285 Lys Leu Leu Thr Thr Pro Asn Pro Glu Glu Asp Lys Asp Leu Gln Leu 290 295 300 Phe Arg Lys Leu Gly Leu Asn Pro Gln Gln Thr Ala Val Leu Ala Gly 305 310 315 320 Asp Asn Glu Gln Gln Gln Arg Leu Glu Gln Ala Leu Met Thr Pro Asp 325 330 335 Thr Asp Glu Tyr Tyr Asn Ala Ala Ala Leu 340 345 <210> 23 <211> 1040 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(222) <223> Mutant bioB gene with frameshift encoding inactive truncated biotin synthase (EC 2.8.1.6) <400> 23 atg gcc acc gcc cac gct gga cat tgt cgc aag tca cag aat tat ttg 48 Met Ala Thr Ala His Ala Gly His Cys Arg Lys Ser Gln Asn Tyr Leu 1 5 10 15 aaa aac cgt tgc tgg atc tgc tgt ttg aag cgc agc agg tgc atc gcc 96 Lys Asn Arg Cys Trp Ile Cys Cys Leu Lys Arg Ser Arg Cys Ile Ala 20 25 30 agc att tcg atc ctc gtc agg tgc agg tca gca cgt tgc tgt cga tta 144 Ser Ile Ser Ile Leu Val Arg Cys Arg Ser Ala Arg Cys Cys Arg Leu 35 40 45 aga ccg gag ctt gtc cgg aag att gca aat act gcc cgc aaa gct cgc 192 Arg Pro Glu Leu Val Arg Lys Ile Ala Asn Thr Ala Arg Lys Ala Arg 50 55 60 gct aca aaa ccg ggc tgg aag ccg agc ggt tgatggaagt tgaacaggtg 242 Ala Thr Lys Pro Gly Trp Lys Pro Ser Gly 65 70 ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat gggcgcggcg 302 tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca gggggtaaaa 362 gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca ggcgcagcgc 422 ctcgcgaacg ccgggctgga ttactacaac cacaacctgg acacctcgcc ggagttttac 482 ggcaatatca tcaccacacg cacttatcag gaacgcctcg atacgctgga aaaagtgcgc 542 gatgccggga tcaaagtctg ttctggcggc attgtgggct taggcgaaac ggtaaaagat 602 cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag cgtgccaatc 662 aacatgctgg tgaaggtgaa aggcacgccg cttgccgata acgatgatgt cgatgccttt 722 gattttattc gcaccattgc ggtcgcgcgg atcatgatgc caacctctta cgtgcgcctt 782 tctgccggac gcgagcagat gaacgaacag actcaggcga tgtgctttat ggcaggcgca 842 aactcgattt tctacggttg caaactgctg accacgccga atccggaaga agataaagac 902 ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct ggcaggggat 962 aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga cgaatattac 1022 aacgcggcag cattatga 1040 <210> 24 <211> 74 <212> PRT <213> Escherichia coli <400> 24 Met Ala Thr Ala His Ala Gly His Cys Arg Lys Ser Gln Asn Tyr Leu 1 5 10 15 Lys Asn Arg Cys Trp Ile Cys Cys Leu Lys Arg Ser Arg Cys Ile Ala 20 25 30 Ser Ile Ser Ile Leu Val Arg Cys Arg Ser Ala Arg Cys Cys Arg Leu 35 40 45 Arg Pro Glu Leu Val Arg Lys Ile Ala Asn Thr Ala Arg Lys Ala Arg 50 55 60 Ala Thr Lys Pro Gly Trp Lys Pro Ser Gly 65 70 <210> 25 <211> 97 <212> DNA <213> Escherichia coli <220> <221> promoter <222> (1)..(97) <223> T5 lacO repressed promoter <400> 25 tcataaaaaa tttatttgct ttgtgagcgg ataacaatta taatagattc aaatcggagg 60 ttctctaact agtatctcta gagctaagga ggtaaat 97 <210> 26 <211> 1041 <212> DNA <213> Candidatus Chloracidobacterium thermophilum B <220> <221> CDS <222> (1)..(1041) <223> bioB gene encoding biotin synthase from Candidatus Chloracidobacterium thermophilum B <400> 26 atg agc cag ccg ctg gtt cgt ttt gat tgg acc cgt gat gaa ctg cgt 48 Met Ser Gln Pro Leu Val Arg Phe Asp Trp Thr Arg Asp Glu Leu Arg 1 5 10 15 gca ctg cat gat ctg ccg ctg ctg gaa ctg att cat cgt gca gca acc 96 Ala Leu His Asp Leu Pro Leu Leu Glu Leu Ile His Arg Ala Ala Thr 20 25 30 gtt cat cgt acc tgt cat gat ccg cag gaa gtt cag gtt tgt cgt ctg 144 Val His Arg Thr Cys His Asp Pro Gln Glu Val Gln Val Cys Arg Leu 35 40 45 att agc att aaa acc ggc ggt tgt ccg gaa gat tgt ggt tat tgt agc 192 Ile Ser Ile Lys Thr Gly Gly Cys Pro Glu Asp Cys Gly Tyr Cys Ser 50 55 60 cag agc gca cat tat gaa acc ggt att gca gca cag ccg ctg ctg gat 240 Gln Ser Ala His Tyr Glu Thr Gly Ile Ala Ala Gln Pro Leu Leu Asp 65 70 75 80 aaa gcc acc gtt gtt gca att gca gaa cgt gca aaa gca cat ggt gtt 288 Lys Ala Thr Val Val Ala Ile Ala Glu Arg Ala Lys Ala His Gly Val 85 90 95 agc cgt gtt tgt ctg ggt gca gca tgg cgt aat gtt cgt gat gat gca 336 Ser Arg Val Cys Leu Gly Ala Ala Trp Arg Asn Val Arg Asp Asp Ala 100 105 110 cag ttt gaa gca gtt ctg gat att gtt cgt agc gtt aat gca ctg ggt 384 Gln Phe Glu Ala Val Leu Asp Ile Val Arg Ser Val Asn Ala Leu Gly 115 120 125 att gaa gtt tgt tgt acc ctg ggt atg ctg acc gaa gcc cag gca cgt 432 Ile Glu Val Cys Cys Thr Leu Gly Met Leu Thr Glu Ala Gln Ala Arg 130 135 140 cgt ctg gaa gaa gca ggt ctg tat gca tat aat cat aat ctg gat acc 480 Arg Leu Glu Glu Ala Gly Leu Tyr Ala Tyr Asn His Asn Leu Asp Thr 145 150 155 160 agc cgt gaa tat tat ggt cgt gtt gtt acc acc cgt acc tat gat gat 528 Ser Arg Glu Tyr Tyr Gly Arg Val Val Thr Thr Arg Thr Tyr Asp Asp 165 170 175 cgc ctg gaa acc ctg gca aat gtt cgc aaa acc ggt gtt acc ctg tgt 576 Arg Leu Glu Thr Leu Ala Asn Val Arg Lys Thr Gly Val Thr Leu Cys 180 185 190 acc ggt ggt att ctg ggt ctg ggt gaa agc acc gat gat cgt att ggt 624 Thr Gly Gly Ile Leu Gly Leu Gly Glu Ser Thr Asp Asp Arg Ile Gly 195 200 205 ctg ctg cat acc ctg gcc acc atg aat ccg cat ccg gaa agc gtt ccg 672 Leu Leu His Thr Leu Ala Thr Met Asn Pro His Pro Glu Ser Val Pro 210 215 220 att aat ctg ctg acc cgt gtt ccg ggt acc ccg atg gaa aat gaa gca 720 Ile Asn Leu Leu Thr Arg Val Pro Gly Thr Pro Met Glu Asn Glu Ala 225 230 235 240 gaa gtt agc gtt tgg gaa acc ctg cgt gtt att gca acc gca cgt att 768 Glu Val Ser Val Trp Glu Thr Leu Arg Val Ile Ala Thr Ala Arg Ile 245 250 255 gca atg ccg cgt agt gtt att cgt ctg agc gca ggt cgt acc cag ctg 816 Ala Met Pro Arg Ser Val Ile Arg Leu Ser Ala Gly Arg Thr Gln Leu 260 265 270 agc gaa gaa gca cag gca ctg tgt ttt ctg gcc ggt gca aat agc att 864 Ser Glu Glu Ala Gln Ala Leu Cys Phe Leu Ala Gly Ala Asn Ser Ile 275 280 285 ttt agc agc gat gca cgt atg atg ctg acc cgc gtt agc ccg acc aat 912 Phe Ser Ser Asp Ala Arg Met Met Leu Thr Arg Val Ser Pro Thr Asn 290 295 300 gat tat gat gaa gat gcc cag ctg ctg aat aaa ctg ggt ctg cat ccg 960 Asp Tyr Asp Glu Asp Ala Gln Leu Leu Asn Lys Leu Gly Leu His Pro 305 310 315 320 cgt gtt ccg ttt aaa gat gca ccg aat gca aaa acc gca ggt tgt gca 1008 Arg Val Pro Phe Lys Asp Ala Pro Asn Ala Lys Thr Ala Gly Cys Ala 325 330 335 agc gca gcc acc gca acc ctg cag gaa aaa taa 1041 Ser Ala Ala Thr Ala Thr Leu Gln Glu Lys 340 345 <210> 27 <211> 346 <212> PRT <213> Candidatus Chloracidobacterium thermophilum B <400> 27 Met Ser Gln Pro Leu Val Arg Phe Asp Trp Thr Arg Asp Glu Leu Arg 1 5 10 15 Ala Leu His Asp Leu Pro Leu Leu Glu Leu Ile His Arg Ala Ala Thr 20 25 30 Val His Arg Thr Cys His Asp Pro Gln Glu Val Gln Val Cys Arg Leu 35 40 45 Ile Ser Ile Lys Thr Gly Gly Cys Pro Glu Asp Cys Gly Tyr Cys Ser 50 55 60 Gln Ser Ala His Tyr Glu Thr Gly Ile Ala Ala Gln Pro Leu Leu Asp 65 70 75 80 Lys Ala Thr Val Val Ala Ile Ala Glu Arg Ala Lys Ala His Gly Val 85 90 95 Ser Arg Val Cys Leu Gly Ala Ala Trp Arg Asn Val Arg Asp Asp Ala 100 105 110 Gln Phe Glu Ala Val Leu Asp Ile Val Arg Ser Val Asn Ala Leu Gly 115 120 125 Ile Glu Val Cys Cys Thr Leu Gly Met Leu Thr Glu Ala Gln Ala Arg 130 135 140 Arg Leu Glu Glu Ala Gly Leu Tyr Ala Tyr Asn His Asn Leu Asp Thr 145 150 155 160 Ser Arg Glu Tyr Tyr Gly Arg Val Val Thr Thr Arg Thr Tyr Asp Asp 165 170 175 Arg Leu Glu Thr Leu Ala Asn Val Arg Lys Thr Gly Val Thr Leu Cys 180 185 190 Thr Gly Gly Ile Leu Gly Leu Gly Glu Ser Thr Asp Asp Arg Ile Gly 195 200 205 Leu Leu His Thr Leu Ala Thr Met Asn Pro His Pro Glu Ser Val Pro 210 215 220 Ile Asn Leu Leu Thr Arg Val Pro Gly Thr Pro Met Glu Asn Glu Ala 225 230 235 240 Glu Val Ser Val Trp Glu Thr Leu Arg Val Ile Ala Thr Ala Arg Ile 245 250 255 Ala Met Pro Arg Ser Val Ile Arg Leu Ser Ala Gly Arg Thr Gln Leu 260 265 270 Ser Glu Glu Ala Gln Ala Leu Cys Phe Leu Ala Gly Ala Asn Ser Ile 275 280 285 Phe Ser Ser Asp Ala Arg Met Met Leu Thr Arg Val Ser Pro Thr Asn 290 295 300 Asp Tyr Asp Glu Asp Ala Gln Leu Leu Asn Lys Leu Gly Leu His Pro 305 310 315 320 Arg Val Pro Phe Lys Asp Ala Pro Asn Ala Lys Thr Ala Gly Cys Ala 325 330 335 Ser Ala Ala Thr Ala Thr Leu Gln Glu Lys 340 345 <210> 28 <211> 1164 <212> DNA <213> Streptomyces lydicus <220> <221> CDS <222> (1)..(1164) <223> bioB gene encoding biotin synthase from Streptomyces lydicus A02 <400> 28 atg ccg tat gtt cgt att aat gca atg gat ctg ctg aat acc ctg gtt 48 Met Pro Tyr Val Arg Ile Asn Ala Met Asp Leu Leu Asn Thr Leu Val 1 5 10 15 gat aaa ggt ctg cgt cgt gaa ctg ccg acc cgt gaa gaa gca ctg gca 96 Asp Lys Gly Leu Arg Arg Glu Leu Pro Thr Arg Glu Glu Ala Leu Ala 20 25 30 gtt ctg gca acc agc gat gat gaa ctg ctg gat gtt gtt gca gca gcg 144 Val Leu Ala Thr Ser Asp Asp Glu Leu Leu Asp Val Val Ala Ala Ala 35 40 45 ggt aaa gtt cgc cgt cag tgg ttt ggt cgt cgt gtt aaa ctg aat tat 192 Gly Lys Val Arg Arg Gln Trp Phe Gly Arg Arg Val Lys Leu Asn Tyr 50 55 60 ctg gtg aat ctg aaa agc ggt ctg tgt ccg gaa gat tgt agc tat tgt 240 Leu Val Asn Leu Lys Ser Gly Leu Cys Pro Glu Asp Cys Ser Tyr Cys 65 70 75 80 agc cag cgt ctg ggt agc aaa gca gaa att ctg aaa tat acc tgg ctg 288 Ser Gln Arg Leu Gly Ser Lys Ala Glu Ile Leu Lys Tyr Thr Trp Leu 85 90 95 aaa ccg gat gat gca agt aaa gca gcc gca gca ggc gtt gcc ggt ggt 336 Lys Pro Asp Asp Ala Ser Lys Ala Ala Ala Ala Gly Val Ala Gly Gly 100 105 110 gca aaa cgt gtt tgt ctg gtt gca agc ggt cgt ggt ccg acc gat aaa 384 Ala Lys Arg Val Cys Leu Val Ala Ser Gly Arg Gly Pro Thr Asp Lys 115 120 125 gat gtt gat cgt gtg agc gaa acc att agc gca att aaa gaa cag aat 432 Asp Val Asp Arg Val Ser Glu Thr Ile Ser Ala Ile Lys Glu Gln Asn 130 135 140 gaa ggt gtg gaa gtt tgt gca tgt ctg ggt ctg ctg agc gat ggt cag 480 Glu Gly Val Glu Val Cys Ala Cys Leu Gly Leu Leu Ser Asp Gly Gln 145 150 155 160 gca gat cgt ctg cgt gca gcc ggt gca gat gca tat aat cat aat ctg 528 Ala Asp Arg Leu Arg Ala Ala Gly Ala Asp Ala Tyr Asn His Asn Leu 165 170 175 aat acc agt gaa gca acc tat ggt gat att tgt acc acc cat gat ttt 576 Asn Thr Ser Glu Ala Thr Tyr Gly Asp Ile Cys Thr Thr His Asp Phe 180 185 190 agc gat cgt gtt agc acc gtt cag cag gca cag gca gca ggt atg agc 624 Ser Asp Arg Val Ser Thr Val Gln Gln Ala Gln Ala Ala Gly Met Ser 195 200 205 gca tgt agc ggt ctg att gca ggt atg ggt gaa agc gat gca gat ctg 672 Ala Cys Ser Gly Leu Ile Ala Gly Met Gly Glu Ser Asp Ala Asp Leu 210 215 220 gtg gat gtg gtt ttt gca ctg cgt gaa ctg gat ccg gat agc gtt ccg 720 Val Asp Val Val Phe Ala Leu Arg Glu Leu Asp Pro Asp Ser Val Pro 225 230 235 240 gtt aat ttt ctg att ccg ttt gaa ggt acc ccg ctg gca aaa gaa tgg 768 Val Asn Phe Leu Ile Pro Phe Glu Gly Thr Pro Leu Ala Lys Glu Trp 245 250 255 aat ctg acc ccg cag cgt gcc ctg cgt att ctg gca atg gtt cgt ttt 816 Asn Leu Thr Pro Gln Arg Ala Leu Arg Ile Leu Ala Met Val Arg Phe 260 265 270 gtt tgt ccg gat gtt gaa gtt cgt ctg gca ggc ggt cgt gaa gtt cat 864 Val Cys Pro Asp Val Glu Val Arg Leu Ala Gly Gly Arg Glu Val His 275 280 285 ctg cgt agc ctg cag ccg ctg gca ctg cat ctg gtt aat agc att ttt 912 Leu Arg Ser Leu Gln Pro Leu Ala Leu His Leu Val Asn Ser Ile Phe 290 295 300 ctg ggt gat tat ctg acc agc gaa ggt cag gcc ggt aaa gaa gat ctg 960 Leu Gly Asp Tyr Leu Thr Ser Glu Gly Gln Ala Gly Lys Glu Asp Leu 305 310 315 320 gcc atg att gcc gat gca ggt ttt gaa gtg gaa ggt aca gat acc acc 1008 Ala Met Ile Ala Asp Ala Gly Phe Glu Val Glu Gly Thr Asp Thr Thr 325 330 335 acc ctg ccg gaa cat cgt aca gat gct gca gtt cag ccg gca ccg gaa 1056 Thr Leu Pro Glu His Arg Thr Asp Ala Ala Val Gln Pro Ala Pro Glu 340 345 350 ccg gca gca gat gca gca gtt cct gca cct cct agt gaa gaa aca cgt 1104 Pro Ala Ala Asp Ala Ala Val Pro Ala Pro Pro Ser Glu Glu Thr Arg 355 360 365 cgt gat ctg gtt agc gtt cgt cgt cgt ggt gca ggt acc gaa ctg ccg 1152 Arg Asp Leu Val Ser Val Arg Arg Arg Gly Ala Gly Thr Glu Leu Pro 370 375 380 ccg aat gca taa 1164 Pro Asn Ala 385 <210> 29 <211> 387 <212> PRT <213> Streptomyces lydicus <400> 29 Met Pro Tyr Val Arg Ile Asn Ala Met Asp Leu Leu Asn Thr Leu Val 1 5 10 15 Asp Lys Gly Leu Arg Arg Glu Leu Pro Thr Arg Glu Glu Ala Leu Ala 20 25 30 Val Leu Ala Thr Ser Asp Asp Glu Leu Leu Asp Val Val Ala Ala Ala 35 40 45 Gly Lys Val Arg Arg Gln Trp Phe Gly Arg Arg Val Lys Leu Asn Tyr 50 55 60 Leu Val Asn Leu Lys Ser Gly Leu Cys Pro Glu Asp Cys Ser Tyr Cys 65 70 75 80 Ser Gln Arg Leu Gly Ser Lys Ala Glu Ile Leu Lys Tyr Thr Trp Leu 85 90 95 Lys Pro Asp Asp Ala Ser Lys Ala Ala Ala Ala Gly Val Ala Gly Gly 100 105 110 Ala Lys Arg Val Cys Leu Val Ala Ser Gly Arg Gly Pro Thr Asp Lys 115 120 125 Asp Val Asp Arg Val Ser Glu Thr Ile Ser Ala Ile Lys Glu Gln Asn 130 135 140 Glu Gly Val Glu Val Cys Ala Cys Leu Gly Leu Leu Ser Asp Gly Gln 145 150 155 160 Ala Asp Arg Leu Arg Ala Ala Gly Ala Asp Ala Tyr Asn His Asn Leu 165 170 175 Asn Thr Ser Glu Ala Thr Tyr Gly Asp Ile Cys Thr Thr His Asp Phe 180 185 190 Ser Asp Arg Val Ser Thr Val Gln Gln Ala Gln Ala Ala Gly Met Ser 195 200 205 Ala Cys Ser Gly Leu Ile Ala Gly Met Gly Glu Ser Asp Ala Asp Leu 210 215 220 Val Asp Val Val Phe Ala Leu Arg Glu Leu Asp Pro Asp Ser Val Pro 225 230 235 240 Val Asn Phe Leu Ile Pro Phe Glu Gly Thr Pro Leu Ala Lys Glu Trp 245 250 255 Asn Leu Thr Pro Gln Arg Ala Leu Arg Ile Leu Ala Met Val Arg Phe 260 265 270 Val Cys Pro Asp Val Glu Val Arg Leu Ala Gly Gly Arg Glu Val His 275 280 285 Leu Arg Ser Leu Gln Pro Leu Ala Leu His Leu Val Asn Ser Ile Phe 290 295 300 Leu Gly Asp Tyr Leu Thr Ser Glu Gly Gln Ala Gly Lys Glu Asp Leu 305 310 315 320 Ala Met Ile Ala Asp Ala Gly Phe Glu Val Glu Gly Thr Asp Thr Thr 325 330 335 Thr Leu Pro Glu His Arg Thr Asp Ala Ala Val Gln Pro Ala Pro Glu 340 345 350 Pro Ala Ala Asp Ala Ala Val Pro Ala Pro Pro Ser Glu Glu Thr Arg 355 360 365 Arg Asp Leu Val Ser Val Arg Arg Arg Gly Ala Gly Thr Glu Leu Pro 370 375 380 Pro Asn Ala 385 <210> 30 <211> 975 <212> DNA <213> Paracoccus denitrificans <220> <221> CDS <222> (1)..(975) <223> bioB gene encoding biotin synthase from Paracoccus denitrificans PD1222 <400> 30 atg att cgt acc gat tgg aca atg gca gaa gca tgg gca att cat gca 48 Met Ile Arg Thr Asp Trp Thr Met Ala Glu Ala Trp Ala Ile His Ala 1 5 10 15 ctg ccg ttt gca gat ctg atg cat cgc gca cag acc ctg cat cgt gca 96 Leu Pro Phe Ala Asp Leu Met His Arg Ala Gln Thr Leu His Arg Ala 20 25 30 cat ttt gat ccg aat gca att gaa acc gca agc ctg ctg agc att aaa 144 His Phe Asp Pro Asn Ala Ile Glu Thr Ala Ser Leu Leu Ser Ile Lys 35 40 45 acc ggt ggt tgt ccg gaa gat tgt ggt tat tgt agc cag agc gca cat 192 Thr Gly Gly Cys Pro Glu Asp Cys Gly Tyr Cys Ser Gln Ser Ala His 50 55 60 cat gat acc ggt gtt aaa gca acc aaa ctg atg ggt acc gaa gaa gtt 240 His Asp Thr Gly Val Lys Ala Thr Lys Leu Met Gly Thr Glu Glu Val 65 70 75 80 ctg gca gca gca cgt cgt gca aaa gca agc ggt gca cag cgt ttt tgt 288 Leu Ala Ala Ala Arg Arg Ala Lys Ala Ser Gly Ala Gln Arg Phe Cys 85 90 95 atg ggt gca gca tgg cgt agc ccg aaa gat cgt gat atg gat aaa ctg 336 Met Gly Ala Ala Trp Arg Ser Pro Lys Asp Arg Asp Met Asp Lys Leu 100 105 110 tgt gat atg gtt cgt ggt gtt gca gaa ctg ggt ctg gaa acc tgt atg 384 Cys Asp Met Val Arg Gly Val Ala Glu Leu Gly Leu Glu Thr Cys Met 115 120 125 acc ctg ggt atg ctg agc ccg gaa cag gtt gca cgt ctg aaa gca gca 432 Thr Leu Gly Met Leu Ser Pro Glu Gln Val Ala Arg Leu Lys Ala Ala 130 135 140 ggt ctg gat ttt tat aat cat aat att gat acc agc ccg gaa tat tat 480 Gly Leu Asp Phe Tyr Asn His Asn Ile Asp Thr Ser Pro Glu Tyr Tyr 145 150 155 160 gcc cag att gcc agc acc cgt aca atg gaa aat cgt ctg gat acc gtt 528 Ala Gln Ile Ala Ser Thr Arg Thr Met Glu Asn Arg Leu Asp Thr Val 165 170 175 gaa cag gtt cgt aaa ggt ggt att aaa gtt tgt tgt ggt ggt att ctg 576 Glu Gln Val Arg Lys Gly Gly Ile Lys Val Cys Cys Gly Gly Ile Leu 180 185 190 ggt atg ggt gaa gca gaa gaa gat cgt att gca atg ctg gtt acc ctg 624 Gly Met Gly Glu Ala Glu Glu Asp Arg Ile Ala Met Leu Val Thr Leu 195 200 205 gca acc ctg ccg gca cat ccg gat agc gtt ccg gtt aat ctg tgg aat 672 Ala Thr Leu Pro Ala His Pro Asp Ser Val Pro Val Asn Leu Trp Asn 210 215 220 gaa att gaa ggt gtt ccg gtt cag gca cgt gca cag gca gtt gat ccg 720 Glu Ile Glu Gly Val Pro Val Gln Ala Arg Ala Gln Ala Val Asp Pro 225 230 235 240 ttt gcc ctg gtt cgt att gtt gca ctg gca cgt att ctg atg ccg gca 768 Phe Ala Leu Val Arg Ile Val Ala Leu Ala Arg Ile Leu Met Pro Ala 245 250 255 agc gtt gtt cgt ctg agc gca ggt cgt acc ggt atg agc gat gaa ctg 816 Ser Val Val Arg Leu Ser Ala Gly Arg Thr Gly Met Ser Asp Glu Leu 260 265 270 cag gca ctg tgt ttt ctg gcg ggt gca aat agc att ttt gtt ggt gat 864 Gln Ala Leu Cys Phe Leu Ala Gly Ala Asn Ser Ile Phe Val Gly Asp 275 280 285 cag ctg ctg acc acc ggt aat ccg gca gca tgg aaa gat cag gat ctg 912 Gln Leu Leu Thr Thr Gly Asn Pro Ala Ala Trp Lys Asp Gln Asp Leu 290 295 300 ctg agt cgt ctg ggt atg cat att gca ccg gca cag gcc cgt ccg cgt 960 Leu Ser Arg Leu Gly Met His Ile Ala Pro Ala Gln Ala Arg Pro Arg 305 310 315 320 gtt gca gca gaa taa 975 Val Ala Ala Glu <210> 31 <211> 324 <212> PRT <213> Paracoccus denitrificans <400> 31 Met Ile Arg Thr Asp Trp Thr Met Ala Glu Ala Trp Ala Ile His Ala 1 5 10 15 Leu Pro Phe Ala Asp Leu Met His Arg Ala Gln Thr Leu His Arg Ala 20 25 30 His Phe Asp Pro Asn Ala Ile Glu Thr Ala Ser Leu Leu Ser Ile Lys 35 40 45 Thr Gly Gly Cys Pro Glu Asp Cys Gly Tyr Cys Ser Gln Ser Ala His 50 55 60 His Asp Thr Gly Val Lys Ala Thr Lys Leu Met Gly Thr Glu Glu Val 65 70 75 80 Leu Ala Ala Ala Arg Arg Ala Lys Ala Ser Gly Ala Gln Arg Phe Cys 85 90 95 Met Gly Ala Ala Trp Arg Ser Pro Lys Asp Arg Asp Met Asp Lys Leu 100 105 110 Cys Asp Met Val Arg Gly Val Ala Glu Leu Gly Leu Glu Thr Cys Met 115 120 125 Thr Leu Gly Met Leu Ser Pro Glu Gln Val Ala Arg Leu Lys Ala Ala 130 135 140 Gly Leu Asp Phe Tyr Asn His Asn Ile Asp Thr Ser Pro Glu Tyr Tyr 145 150 155 160 Ala Gln Ile Ala Ser Thr Arg Thr Met Glu Asn Arg Leu Asp Thr Val 165 170 175 Glu Gln Val Arg Lys Gly Gly Ile Lys Val Cys Cys Gly Gly Ile Leu 180 185 190 Gly Met Gly Glu Ala Glu Glu Asp Arg Ile Ala Met Leu Val Thr Leu 195 200 205 Ala Thr Leu Pro Ala His Pro Asp Ser Val Pro Val Asn Leu Trp Asn 210 215 220 Glu Ile Glu Gly Val Pro Val Gln Ala Arg Ala Gln Ala Val Asp Pro 225 230 235 240 Phe Ala Leu Val Arg Ile Val Ala Leu Ala Arg Ile Leu Met Pro Ala 245 250 255 Ser Val Val Arg Leu Ser Ala Gly Arg Thr Gly Met Ser Asp Glu Leu 260 265 270 Gln Ala Leu Cys Phe Leu Ala Gly Ala Asn Ser Ile Phe Val Gly Asp 275 280 285 Gln Leu Leu Thr Thr Gly Asn Pro Ala Ala Trp Lys Asp Gln Asp Leu 290 295 300 Leu Ser Arg Leu Gly Met His Ile Ala Pro Ala Gln Ala Arg Pro Arg 305 310 315 320 Val Ala Ala Glu <210> 32 <211> 963 <212> DNA <213> Paracoccus denitrificans <220> <221> CDS <222> (1)..(963) <223> bioB gene encoding biotin synthase from Paracoccus denitrificans PD1222(2) <400> 32 atg ccg cag cag agc cgt agc gca gca gaa att tat cat cag ccg ctg 48 Met Pro Gln Gln Ser Arg Ser Ala Ala Glu Ile Tyr His Gln Pro Leu 1 5 10 15 atg gat ctg ctg ttt cag gca cag acc gtt cat cgt gca cat ttt gat 96 Met Asp Leu Leu Phe Gln Ala Gln Thr Val His Arg Ala His Phe Asp 20 25 30 ccg aat gtt gtt cag tgt agc aaa ctg ctg agc att aaa acc ggt ggt 144 Pro Asn Val Val Gln Cys Ser Lys Leu Leu Ser Ile Lys Thr Gly Gly 35 40 45 tgt ccg gaa gat tgt gca tat tgt agc cag agc gca cgt aat ggt agc 192 Cys Pro Glu Asp Cys Ala Tyr Cys Ser Gln Ser Ala Arg Asn Gly Ser 50 55 60 gaa ctg agc gca agc aaa ctg atg gaa gtt cag cgt gtt ctg gca gaa 240 Glu Leu Ser Ala Ser Lys Leu Met Glu Val Gln Arg Val Leu Ala Glu 65 70 75 80 gca cgt cgt gca aaa gaa gcc ggt gca acc cgt tat tgt atg ggt gca 288 Ala Arg Arg Ala Lys Glu Ala Gly Ala Thr Arg Tyr Cys Met Gly Ala 85 90 95 gca tgg cgt agc ccg aaa gaa cgt gat atg ccg gca gtt ctg gca atg 336 Ala Trp Arg Ser Pro Lys Glu Arg Asp Met Pro Ala Val Leu Ala Met 100 105 110 att cgt ggt gtt aaa gca atg ggt atg gaa acc tgt atg acc ctg ggt 384 Ile Arg Gly Val Lys Ala Met Gly Met Glu Thr Cys Met Thr Leu Gly 115 120 125 atg ctg gat gca gat cag gca ctg cgt ctg aaa gat gcc ggt ctg gat 432 Met Leu Asp Ala Asp Gln Ala Leu Arg Leu Lys Asp Ala Gly Leu Asp 130 135 140 tat tat aac cat aat att gat acc agc gaa cgc tat tat agc gaa att 480 Tyr Tyr Asn His Asn Ile Asp Thr Ser Glu Arg Tyr Tyr Ser Glu Ile 145 150 155 160 att acc acc cgc acc ttt cag gat cgc att gaa acc ctg gaa cgt gtt 528 Ile Thr Thr Arg Thr Phe Gln Asp Arg Ile Glu Thr Leu Glu Arg Val 165 170 175 cag gca gca ggt att aat gtt tgt gcc ggt ggt att gtt ggt atg ggt 576 Gln Ala Ala Gly Ile Asn Val Cys Ala Gly Gly Ile Val Gly Met Gly 180 185 190 gaa acc gca gaa gat cgt att agc atg ctg gaa acc ctg gca ggt ctg 624 Glu Thr Ala Glu Asp Arg Ile Ser Met Leu Glu Thr Leu Ala Gly Leu 195 200 205 gaa gtg ccg ccg cag agc gtt ccg att aat atg ctg atg ccg atg gca 672 Glu Val Pro Pro Gln Ser Val Pro Ile Asn Met Leu Met Pro Met Ala 210 215 220 ggt acc ccg ctg gca gat gtt ccg cgt ctg gat gca att gaa atg gtt 720 Gly Thr Pro Leu Ala Asp Val Pro Arg Leu Asp Ala Ile Glu Met Val 225 230 235 240 cgt acc att gca acc gca cgt att ctg atg ccg gca agc tat gtt cgt 768 Arg Thr Ile Ala Thr Ala Arg Ile Leu Met Pro Ala Ser Tyr Val Arg 245 250 255 ctg agc gca ggt cgt agc gaa atg agc gat gaa atg cag gca atg tgt 816 Leu Ser Ala Gly Arg Ser Glu Met Ser Asp Glu Met Gln Ala Met Cys 260 265 270 ttt ttt gca ggc gca aat agc att ttt gtt ggt gat acc ctg ctg acc 864 Phe Phe Ala Gly Ala Asn Ser Ile Phe Val Gly Asp Thr Leu Leu Thr 275 280 285 gca ggt aat ccg gat gaa gat aaa gat gca ctg ctg ttt gca aaa ctg 912 Ala Gly Asn Pro Asp Glu Asp Lys Asp Ala Leu Leu Phe Ala Lys Leu 290 295 300 ggt ctg cgt gca gaa gtt ccg gaa gca agc cag gaa ggt tgt gca gca 960 Gly Leu Arg Ala Glu Val Pro Glu Ala Ser Gln Glu Gly Cys Ala Ala 305 310 315 320 taa 963 <210> 33 <211> 320 <212> PRT <213> Paracoccus denitrificans <400> 33 Met Pro Gln Gln Ser Arg Ser Ala Ala Glu Ile Tyr His Gln Pro Leu 1 5 10 15 Met Asp Leu Leu Phe Gln Ala Gln Thr Val His Arg Ala His Phe Asp 20 25 30 Pro Asn Val Val Gln Cys Ser Lys Leu Leu Ser Ile Lys Thr Gly Gly 35 40 45 Cys Pro Glu Asp Cys Ala Tyr Cys Ser Gln Ser Ala Arg Asn Gly Ser 50 55 60 Glu Leu Ser Ala Ser Lys Leu Met Glu Val Gln Arg Val Leu Ala Glu 65 70 75 80 Ala Arg Arg Ala Lys Glu Ala Gly Ala Thr Arg Tyr Cys Met Gly Ala 85 90 95 Ala Trp Arg Ser Pro Lys Glu Arg Asp Met Pro Ala Val Leu Ala Met 100 105 110 Ile Arg Gly Val Lys Ala Met Gly Met Glu Thr Cys Met Thr Leu Gly 115 120 125 Met Leu Asp Ala Asp Gln Ala Leu Arg Leu Lys Asp Ala Gly Leu Asp 130 135 140 Tyr Tyr Asn His Asn Ile Asp Thr Ser Glu Arg Tyr Tyr Ser Glu Ile 145 150 155 160 Ile Thr Thr Arg Thr Phe Gln Asp Arg Ile Glu Thr Leu Glu Arg Val 165 170 175 Gln Ala Ala Gly Ile Asn Val Cys Ala Gly Gly Ile Val Gly Met Gly 180 185 190 Glu Thr Ala Glu Asp Arg Ile Ser Met Leu Glu Thr Leu Ala Gly Leu 195 200 205 Glu Val Pro Pro Gln Ser Val Pro Ile Asn Met Leu Met Pro Met Ala 210 215 220 Gly Thr Pro Leu Ala Asp Val Pro Arg Leu Asp Ala Ile Glu Met Val 225 230 235 240 Arg Thr Ile Ala Thr Ala Arg Ile Leu Met Pro Ala Ser Tyr Val Arg 245 250 255 Leu Ser Ala Gly Arg Ser Glu Met Ser Asp Glu Met Gln Ala Met Cys 260 265 270 Phe Phe Ala Gly Ala Asn Ser Ile Phe Val Gly Asp Thr Leu Leu Thr 275 280 285 Ala Gly Asn Pro Asp Glu Asp Lys Asp Ala Leu Leu Phe Ala Lys Leu 290 295 300 Gly Leu Arg Ala Glu Val Pro Glu Ala Ser Gln Glu Gly Cys Ala Ala 305 310 315 320 <210> 34 <211> 987 <212> DNA <213> Agrobacterium vitis <220> <221> CDS <222> (1)..(987) <223> bioB gene encoding biotin synthase from Agrobacterium vitis S4 <400> 34 atg agc gaa gca gcc ggt gaa att cgt aat gat tgg agc gtt gaa gaa 48 Met Ser Glu Ala Ala Gly Glu Ile Arg Asn Asp Trp Ser Val Glu Glu 1 5 10 15 att gtg acc ctg cat aat ctg ccg ctg ctg gaa ctg att ggt cat gca 96 Ile Val Thr Leu His Asn Leu Pro Leu Leu Glu Leu Ile Gly His Ala 20 25 30 aat gca gtt cat ggt cgt cat cat aat ccg aat gtt gtt cag aaa gca 144 Asn Ala Val His Gly Arg His His Asn Pro Asn Val Val Gln Lys Ala 35 40 45 agc ctg ctg agc att aaa acc ggt ggt tgt ccg gaa gat tgt gca tat 192 Ser Leu Leu Ser Ile Lys Thr Gly Gly Cys Pro Glu Asp Cys Ala Tyr 50 55 60 tgt ccg cag agc gca cat cat cgt gaa gtt aaa ctg acc aaa gat cgt 240 Cys Pro Gln Ser Ala His His Arg Glu Val Lys Leu Thr Lys Asp Arg 65 70 75 80 ctg atg cag ccg gaa acc gtt ctg gca ctg gca aaa cgt gca aaa gat 288 Leu Met Gln Pro Glu Thr Val Leu Ala Leu Ala Lys Arg Ala Lys Asp 85 90 95 gcc ggt gca gaa cgt ttt tgt atg ggt gca gca tgg cgt cag gtt cgt 336 Ala Gly Ala Glu Arg Phe Cys Met Gly Ala Ala Trp Arg Gln Val Arg 100 105 110 gat ggt aaa gaa ttt gat gca gtt ctg aca atg gtt cgt ggt gtg cgt 384 Asp Gly Lys Glu Phe Asp Ala Val Leu Thr Met Val Arg Gly Val Arg 115 120 125 gat ctg ggt atg gaa gca tgt gtt acc ctg ggt atg ctg gaa aaa cat 432 Asp Leu Gly Met Glu Ala Cys Val Thr Leu Gly Met Leu Glu Lys His 130 135 140 cag gcc gaa aaa ctg gca gaa gca ggt ctg acc gca tat aat cat aat 480 Gln Ala Glu Lys Leu Ala Glu Ala Gly Leu Thr Ala Tyr Asn His Asn 145 150 155 160 ctg gat acc agc ccg gaa ttt tat ggc gaa att att acc acc cgt agc 528 Leu Asp Thr Ser Pro Glu Phe Tyr Gly Glu Ile Ile Thr Thr Arg Ser 165 170 175 tat gca gat cgt ctg gaa acc ctg agc att gtt cgt agc ttt ggt att 576 Tyr Ala Asp Arg Leu Glu Thr Leu Ser Ile Val Arg Ser Phe Gly Ile 180 185 190 gat ctg tgt tgt ggt ggt att att ggt atg ggt gaa acc att cgt gat 624 Asp Leu Cys Cys Gly Gly Ile Ile Gly Met Gly Glu Thr Ile Arg Asp 195 200 205 cgt gca agt atg ctg cag gtt ctg gca agc atg cgt ccg cat ccg gaa 672 Arg Ala Ser Met Leu Gln Val Leu Ala Ser Met Arg Pro His Pro Glu 210 215 220 agt gtt ccg att aat gca ctg gtt ccg gtt gaa ggt acc ccg ctg gca 720 Ser Val Pro Ile Asn Ala Leu Val Pro Val Glu Gly Thr Pro Leu Ala 225 230 235 240 gca atg ccg cgt att gat ccg ctg gaa ctg gtt cgt atg gtt gca acc 768 Ala Met Pro Arg Ile Asp Pro Leu Glu Leu Val Arg Met Val Ala Thr 245 250 255 gca cgt att gtt atg ccg aaa agc acc gtt cgt ctg agc gca ggt cgt 816 Ala Arg Ile Val Met Pro Lys Ser Thr Val Arg Leu Ser Ala Gly Arg 260 265 270 agc acc ctg aat cgt gaa gca cag att ctg tgt ctg gtt agc ggt gca 864 Ser Thr Leu Asn Arg Glu Ala Gln Ile Leu Cys Leu Val Ser Gly Ala 275 280 285 aat agc gtt ttt tat ggt gat acc ctg ctg acc acc ccg aat gca ggt 912 Asn Ser Val Phe Tyr Gly Asp Thr Leu Leu Thr Thr Pro Asn Ala Gly 290 295 300 att ggt gaa gat gaa gca ctg ttt gca gca att ggt gca ctg ccg cat 960 Ile Gly Glu Asp Glu Ala Leu Phe Ala Ala Ile Gly Ala Leu Pro His 305 310 315 320 gaa gca gca ccg ctg gcc gca gaa taa 987 Glu Ala Ala Pro Leu Ala Ala Glu 325 <210> 35 <211> 328 <212> PRT <213> Agrobacterium vitis <400> 35 Met Ser Glu Ala Ala Gly Glu Ile Arg Asn Asp Trp Ser Val Glu Glu 1 5 10 15 Ile Val Thr Leu His Asn Leu Pro Leu Leu Glu Leu Ile Gly His Ala 20 25 30 Asn Ala Val His Gly Arg His His Asn Pro Asn Val Val Gln Lys Ala 35 40 45 Ser Leu Leu Ser Ile Lys Thr Gly Gly Cys Pro Glu Asp Cys Ala Tyr 50 55 60 Cys Pro Gln Ser Ala His His Arg Glu Val Lys Leu Thr Lys Asp Arg 65 70 75 80 Leu Met Gln Pro Glu Thr Val Leu Ala Leu Ala Lys Arg Ala Lys Asp 85 90 95 Ala Gly Ala Glu Arg Phe Cys Met Gly Ala Ala Trp Arg Gln Val Arg 100 105 110 Asp Gly Lys Glu Phe Asp Ala Val Leu Thr Met Val Arg Gly Val Arg 115 120 125 Asp Leu Gly Met Glu Ala Cys Val Thr Leu Gly Met Leu Glu Lys His 130 135 140 Gln Ala Glu Lys Leu Ala Glu Ala Gly Leu Thr Ala Tyr Asn His Asn 145 150 155 160 Leu Asp Thr Ser Pro Glu Phe Tyr Gly Glu Ile Ile Thr Thr Arg Ser 165 170 175 Tyr Ala Asp Arg Leu Glu Thr Leu Ser Ile Val Arg Ser Phe Gly Ile 180 185 190 Asp Leu Cys Cys Gly Gly Ile Ile Gly Met Gly Glu Thr Ile Arg Asp 195 200 205 Arg Ala Ser Met Leu Gln Val Leu Ala Ser Met Arg Pro His Pro Glu 210 215 220 Ser Val Pro Ile Asn Ala Leu Val Pro Val Glu Gly Thr Pro Leu Ala 225 230 235 240 Ala Met Pro Arg Ile Asp Pro Leu Glu Leu Val Arg Met Val Ala Thr 245 250 255 Ala Arg Ile Val Met Pro Lys Ser Thr Val Arg Leu Ser Ala Gly Arg 260 265 270 Ser Thr Leu Asn Arg Glu Ala Gln Ile Leu Cys Leu Val Ser Gly Ala 275 280 285 Asn Ser Val Phe Tyr Gly Asp Thr Leu Leu Thr Thr Pro Asn Ala Gly 290 295 300 Ile Gly Glu Asp Glu Ala Leu Phe Ala Ala Ile Gly Ala Leu Pro His 305 310 315 320 Glu Ala Ala Pro Leu Ala Ala Glu 325 <210> 36 <211> 957 <212> DNA <213> Ruegeria pomeroyi <220> <221> CDS <222> (1)..(957) <223> bioB gene encoding biotin synthase from Ruegeria pomeroyi DSS-3 <400> 36 atg gcc gaa gca att cgt agc gat tgg agc gtt gat gaa gtt gaa gca 48 Met Ala Glu Ala Ile Arg Ser Asp Trp Ser Val Asp Glu Val Glu Ala 1 5 10 15 ctg ctg cgt ctg ccg ctg ctg gat ctg gtt ggt cgt gca aat ggt gtt 96 Leu Leu Arg Leu Pro Leu Leu Asp Leu Val Gly Arg Ala Asn Gly Val 20 25 30 cat cgt gca cat cat gca ccg gat gat att cag aaa gca agc ctg ctg 144 His Arg Ala His His Ala Pro Asp Asp Ile Gln Lys Ala Ser Leu Leu 35 40 45 agc att aaa acc ggt ggt tgt ccg gaa gat tgt gca tat tgc ccg cag 192 Ser Ile Lys Thr Gly Gly Cys Pro Glu Asp Cys Ala Tyr Cys Pro Gln 50 55 60 agc gca cat cat cgt gaa gtg gaa ctg acc cgt gaa aaa ctg atg aat 240 Ser Ala His His Arg Glu Val Glu Leu Thr Arg Glu Lys Leu Met Asn 65 70 75 80 ccg gat cat gtt gtt agc ctg gca cgt cgt gcc cag cgt gcc ggt gcc 288 Pro Asp His Val Val Ser Leu Ala Arg Arg Ala Gln Arg Ala Gly Ala 85 90 95 gaa cgt ttt tgt atg ggt gca gca tgg cgt cag gtt cgt gat ggt gca 336 Glu Arg Phe Cys Met Gly Ala Ala Trp Arg Gln Val Arg Asp Gly Ala 100 105 110 gaa ttt gat aat gtt ctg gca atg gtt cgt ggt gtt cgt gca ctg ggt 384 Glu Phe Asp Asn Val Leu Ala Met Val Arg Gly Val Arg Ala Leu Gly 115 120 125 atg gaa gca tgt gtt acc ctg ggt atg ctg cgt ccg cat cag gca cag 432 Met Glu Ala Cys Val Thr Leu Gly Met Leu Arg Pro His Gln Ala Gln 130 135 140 cgt ctg gca gaa gca ggt ctg acc gca tat aat cat aat ctg gat acc 480 Arg Leu Ala Glu Ala Gly Leu Thr Ala Tyr Asn His Asn Leu Asp Thr 145 150 155 160 agc ccg gaa ttt tat ggt cag att att ggt acc cgt acc tat cag gat 528 Ser Pro Glu Phe Tyr Gly Gln Ile Ile Gly Thr Arg Thr Tyr Gln Asp 165 170 175 cgt ctg gat acc ctg gca tat tgt cgt gat gca ggt att gaa ctg tgt 576 Arg Leu Asp Thr Leu Ala Tyr Cys Arg Asp Ala Gly Ile Glu Leu Cys 180 185 190 tgt ggt ggt att att ggc atg ggt gaa agc ctg cgt gat cgt gca gca 624 Cys Gly Gly Ile Ile Gly Met Gly Glu Ser Leu Arg Asp Arg Ala Ala 195 200 205 atg ctg cag gtt ctg gcc aat ttt gca ccg cat ccg gaa agc gtt ccg 672 Met Leu Gln Val Leu Ala Asn Phe Ala Pro His Pro Glu Ser Val Pro 210 215 220 att aat gca ctg att ccg att gaa ggt acc ccg ctg gca cat cgt gaa 720 Ile Asn Ala Leu Ile Pro Ile Glu Gly Thr Pro Leu Ala His Arg Glu 225 230 235 240 cgt gtt ggt att ttt gat ctg gtt cgt atg gtt gca acc gca cgt att 768 Arg Val Gly Ile Phe Asp Leu Val Arg Met Val Ala Thr Ala Arg Ile 245 250 255 att atg ccg ctg acc cgt gtt cgt ctg agc gca ggt cgt agt gat ttt 816 Ile Met Pro Leu Thr Arg Val Arg Leu Ser Ala Gly Arg Ser Asp Phe 260 265 270 agc gcc gca gaa cag gca ctg tgt ttt ctg gcg ggt gca aat agc gtt 864 Ser Ala Ala Glu Gln Ala Leu Cys Phe Leu Ala Gly Ala Asn Ser Val 275 280 285 ttt tat ggt gat gtt ctg ctg acc gca ccg aat gca ggt acc ggt gca 912 Phe Tyr Gly Asp Val Leu Leu Thr Ala Pro Asn Ala Gly Thr Gly Ala 290 295 300 gat gca gaa ctg ttt gca gca ctg ggt gca ctg gaa acc gca taa 957 Asp Ala Glu Leu Phe Ala Ala Leu Gly Ala Leu Glu Thr Ala 305 310 315 <210> 37 <211> 318 <212> PRT <213> Ruegeria pomeroyi <400> 37 Met Ala Glu Ala Ile Arg Ser Asp Trp Ser Val Asp Glu Val Glu Ala 1 5 10 15 Leu Leu Arg Leu Pro Leu Leu Asp Leu Val Gly Arg Ala Asn Gly Val 20 25 30 His Arg Ala His His Ala Pro Asp Asp Ile Gln Lys Ala Ser Leu Leu 35 40 45 Ser Ile Lys Thr Gly Gly Cys Pro Glu Asp Cys Ala Tyr Cys Pro Gln 50 55 60 Ser Ala His His Arg Glu Val Glu Leu Thr Arg Glu Lys Leu Met Asn 65 70 75 80 Pro Asp His Val Val Ser Leu Ala Arg Arg Ala Gln Arg Ala Gly Ala 85 90 95 Glu Arg Phe Cys Met Gly Ala Ala Trp Arg Gln Val Arg Asp Gly Ala 100 105 110 Glu Phe Asp Asn Val Leu Ala Met Val Arg Gly Val Arg Ala Leu Gly 115 120 125 Met Glu Ala Cys Val Thr Leu Gly Met Leu Arg Pro His Gln Ala Gln 130 135 140 Arg Leu Ala Glu Ala Gly Leu Thr Ala Tyr Asn His Asn Leu Asp Thr 145 150 155 160 Ser Pro Glu Phe Tyr Gly Gln Ile Ile Gly Thr Arg Thr Tyr Gln Asp 165 170 175 Arg Leu Asp Thr Leu Ala Tyr Cys Arg Asp Ala Gly Ile Glu Leu Cys 180 185 190 Cys Gly Gly Ile Ile Gly Met Gly Glu Ser Leu Arg Asp Arg Ala Ala 195 200 205 Met Leu Gln Val Leu Ala Asn Phe Ala Pro His Pro Glu Ser Val Pro 210 215 220 Ile Asn Ala Leu Ile Pro Ile Glu Gly Thr Pro Leu Ala His Arg Glu 225 230 235 240 Arg Val Gly Ile Phe Asp Leu Val Arg Met Val Ala Thr Ala Arg Ile 245 250 255 Ile Met Pro Leu Thr Arg Val Arg Leu Ser Ala Gly Arg Ser Asp Phe 260 265 270 Ser Ala Ala Glu Gln Ala Leu Cys Phe Leu Ala Gly Ala Asn Ser Val 275 280 285 Phe Tyr Gly Asp Val Leu Leu Thr Ala Pro Asn Ala Gly Thr Gly Ala 290 295 300 Asp Ala Glu Leu Phe Ala Ala Leu Gly Ala Leu Glu Thr Ala 305 310 315 <210> 38 <211> 1020 <212> DNA <213> Agrobacterium fabrum <220> <221> CDS <222> (1)..(1020) <223> bioB gene encoding biotin synthase from Agrobacterium fabrum str. C58 <400> 38 atg gat cag ctg gca acc cag att gat ggt aaa ccg gca agc att ccg 48 Met Asp Gln Leu Ala Thr Gln Ile Asp Gly Lys Pro Ala Ser Ile Pro 1 5 10 15 gca gtt gaa acc agc agc agc ctg gaa gaa gcc aaa att att tat aat 96 Ala Val Glu Thr Ser Ser Ser Leu Glu Glu Ala Lys Ile Ile Tyr Asn 20 25 30 ctg ccg ttt aat gat ctg ctg ttt cgc gcc cag cag gtt cat cgt tgt 144 Leu Pro Phe Asn Asp Leu Leu Phe Arg Ala Gln Gln Val His Arg Cys 35 40 45 cat ttt gat gcc aat gca att cag atg agc cgt ctg ctg agc att aaa 192 His Phe Asp Ala Asn Ala Ile Gln Met Ser Arg Leu Leu Ser Ile Lys 50 55 60 acc ggc ggt tgt ccg gaa gat tgt agc tat tgt agc cag agc gca cgt 240 Thr Gly Gly Cys Pro Glu Asp Cys Ser Tyr Cys Ser Gln Ser Ala Arg 65 70 75 80 aat ccg acc ggt ctg aaa gca agc aaa ctg atg gaa gtt gaa cgt gtt 288 Asn Pro Thr Gly Leu Lys Ala Ser Lys Leu Met Glu Val Glu Arg Val 85 90 95 ctg gca gaa gca cgt aaa gca aaa gaa ggt ggt gca acc cgt tat tgt 336 Leu Ala Glu Ala Arg Lys Ala Lys Glu Gly Gly Ala Thr Arg Tyr Cys 100 105 110 atg ggt gca gca tgg cgt aat ccg aaa gaa cgt gat atg gaa gca gtt 384 Met Gly Ala Ala Trp Arg Asn Pro Lys Glu Arg Asp Met Glu Ala Val 115 120 125 gtt gca atg gtt gaa ggt gtt aaa gca ctg gat atg gaa acc tgt atg 432 Val Ala Met Val Glu Gly Val Lys Ala Leu Asp Met Glu Thr Cys Met 130 135 140 acc ctg ggt atg ctg acc ccg gaa cag agc gaa cgt ctg gca gat gca 480 Thr Leu Gly Met Leu Thr Pro Glu Gln Ser Glu Arg Leu Ala Asp Ala 145 150 155 160 ggt ctg gat tat tat aat cat aat gtg gat acc agc gaa cgt ttt tat 528 Gly Leu Asp Tyr Tyr Asn His Asn Val Asp Thr Ser Glu Arg Phe Tyr 165 170 175 agc gaa att att acc acc cgt acc ttt gaa gat cgc ctg gaa acc ctg 576 Ser Glu Ile Ile Thr Thr Arg Thr Phe Glu Asp Arg Leu Glu Thr Leu 180 185 190 gcc aat gtt cgt gat gca ggt att aaa gtt tgt gca ggc ggt att ctg 624 Ala Asn Val Arg Asp Ala Gly Ile Lys Val Cys Ala Gly Gly Ile Leu 195 200 205 ggt atg ggt gaa acc gtg gaa gat cgt att agc atg ctg gtt acc ctg 672 Gly Met Gly Glu Thr Val Glu Asp Arg Ile Ser Met Leu Val Thr Leu 210 215 220 gca aat ctg ccg gtt ccg ccg gaa agc gtt ccg att aat atg ctg att 720 Ala Asn Leu Pro Val Pro Pro Glu Ser Val Pro Ile Asn Met Leu Ile 225 230 235 240 ccg att ccg ggt agc aaa ctg gca aat gca gat ccg gtt gat ccg att 768 Pro Ile Pro Gly Ser Lys Leu Ala Asn Ala Asp Pro Val Asp Pro Ile 245 250 255 gat ttt gtt cgt acc att gca ctg gca cgt att ctg atg ccg cgt agc 816 Asp Phe Val Arg Thr Ile Ala Leu Ala Arg Ile Leu Met Pro Arg Ser 260 265 270 cat gtt cgt ctg agc gca ggt cgt acc gaa atg agc gat gaa acc cag 864 His Val Arg Leu Ser Ala Gly Arg Thr Glu Met Ser Asp Glu Thr Gln 275 280 285 gca ctg tgt ttt ctg gcc ggt gca aat agc att ttt att ggt gaa acc 912 Ala Leu Cys Phe Leu Ala Gly Ala Asn Ser Ile Phe Ile Gly Glu Thr 290 295 300 ctg ctg acc gca gat aat ccg ggt gaa gat cat gat acc gca ctg ttt 960 Leu Leu Thr Ala Asp Asn Pro Gly Glu Asp His Asp Thr Ala Leu Phe 305 310 315 320 cgt cgt ctg ggt ctg aaa ccg atg gaa ctg cag agc agc gaa gcc ggt 1008 Arg Arg Leu Gly Leu Lys Pro Met Glu Leu Gln Ser Ser Glu Ala Gly 325 330 335 ggt tgt cgt taa 1020 Gly Cys Arg <210> 39 <211> 339 <212> PRT <213> Agrobacterium fabrum <400> 39 Met Asp Gln Leu Ala Thr Gln Ile Asp Gly Lys Pro Ala Ser Ile Pro 1 5 10 15 Ala Val Glu Thr Ser Ser Ser Leu Glu Glu Ala Lys Ile Ile Tyr Asn 20 25 30 Leu Pro Phe Asn Asp Leu Leu Phe Arg Ala Gln Gln Val His Arg Cys 35 40 45 His Phe Asp Ala Asn Ala Ile Gln Met Ser Arg Leu Leu Ser Ile Lys 50 55 60 Thr Gly Gly Cys Pro Glu Asp Cys Ser Tyr Cys Ser Gln Ser Ala Arg 65 70 75 80 Asn Pro Thr Gly Leu Lys Ala Ser Lys Leu Met Glu Val Glu Arg Val 85 90 95 Leu Ala Glu Ala Arg Lys Ala Lys Glu Gly Gly Ala Thr Arg Tyr Cys 100 105 110 Met Gly Ala Ala Trp Arg Asn Pro Lys Glu Arg Asp Met Glu Ala Val 115 120 125 Val Ala Met Val Glu Gly Val Lys Ala Leu Asp Met Glu Thr Cys Met 130 135 140 Thr Leu Gly Met Leu Thr Pro Glu Gln Ser Glu Arg Leu Ala Asp Ala 145 150 155 160 Gly Leu Asp Tyr Tyr Asn His Asn Val Asp Thr Ser Glu Arg Phe Tyr 165 170 175 Ser Glu Ile Ile Thr Thr Arg Thr Phe Glu Asp Arg Leu Glu Thr Leu 180 185 190 Ala Asn Val Arg Asp Ala Gly Ile Lys Val Cys Ala Gly Gly Ile Leu 195 200 205 Gly Met Gly Glu Thr Val Glu Asp Arg Ile Ser Met Leu Val Thr Leu 210 215 220 Ala Asn Leu Pro Val Pro Pro Glu Ser Val Pro Ile Asn Met Leu Ile 225 230 235 240 Pro Ile Pro Gly Ser Lys Leu Ala Asn Ala Asp Pro Val Asp Pro Ile 245 250 255 Asp Phe Val Arg Thr Ile Ala Leu Ala Arg Ile Leu Met Pro Arg Ser 260 265 270 His Val Arg Leu Ser Ala Gly Arg Thr Glu Met Ser Asp Glu Thr Gln 275 280 285 Ala Leu Cys Phe Leu Ala Gly Ala Asn Ser Ile Phe Ile Gly Glu Thr 290 295 300 Leu Leu Thr Ala Asp Asn Pro Gly Glu Asp His Asp Thr Ala Leu Phe 305 310 315 320 Arg Arg Leu Gly Leu Lys Pro Met Glu Leu Gln Ser Ser Glu Ala Gly 325 330 335 Gly Cys Arg <210> 40 <211> 954 <212> DNA <213> Wolbachia endosymbiont of Cimex lectularius <220> <221> CDS <222> (1)..(954) <223> bioB gene encoding biotin synthase from Wolbachia endosymbiont of Cimex lectularius <400> 40 atg acc gaa gaa tgg acc ttt gcc aaa gca gat cag att ttt aat ttt 48 Met Thr Glu Glu Trp Thr Phe Ala Lys Ala Asp Gln Ile Phe Asn Phe 1 5 10 15 ccg ttt ccg gaa ctg att tat att gca cag acc gaa cat cgc aaa cag 96 Pro Phe Pro Glu Leu Ile Tyr Ile Ala Gln Thr Glu His Arg Lys Gln 20 25 30 ttt aat ccg agc gaa gtg cag att agc acc ctg ctg agc att aaa acc 144 Phe Asn Pro Ser Glu Val Gln Ile Ser Thr Leu Leu Ser Ile Lys Thr 35 40 45 ggt agc tgt ccg gaa aat tgt agc tat tgt ccg cag agc gca cat tat 192 Gly Ser Cys Pro Glu Asn Cys Ser Tyr Cys Pro Gln Ser Ala His Tyr 50 55 60 aat acc ggt ctg cag aaa aaa ccg ctg ctg gaa att gca gaa gtt att 240 Asn Thr Gly Leu Gln Lys Lys Pro Leu Leu Glu Ile Ala Glu Val Ile 65 70 75 80 gaa gca gca aaa tgt gca aaa gaa gca ggt agc acc cgt ttt tgt atg 288 Glu Ala Ala Lys Cys Ala Lys Glu Ala Gly Ser Thr Arg Phe Cys Met 85 90 95 ggt gca gca tgg cgt ggt ccg cgt gat cag gat ctg aaa gtt gtt tgt 336 Gly Ala Ala Trp Arg Gly Pro Arg Asp Gln Asp Leu Lys Val Val Cys 100 105 110 gaa atg att cgt gaa gtt aaa aaa ctg ggt ctg gaa acc tgt gtt acc 384 Glu Met Ile Arg Glu Val Lys Lys Leu Gly Leu Glu Thr Cys Val Thr 115 120 125 ctg ggt ctg ctg aaa gat cat cag gcc aac atg ctg aaa gaa gcc ggt 432 Leu Gly Leu Leu Lys Asp His Gln Ala Asn Met Leu Lys Glu Ala Gly 130 135 140 ctg gat ttt tat aac cat aac atc gat acc agc gaa gaa tat tat aac 480 Leu Asp Phe Tyr Asn His Asn Ile Asp Thr Ser Glu Glu Tyr Tyr Asn 145 150 155 160 aaa gtg atc acc acc cgt acc ttt cag gat cgt ctg gaa acc ctg gaa 528 Lys Val Ile Thr Thr Arg Thr Phe Gln Asp Arg Leu Glu Thr Leu Glu 165 170 175 tgt gtt cgt gca agc ggt att aaa gtt tgt tgt ggt ggt att ctg ggt 576 Cys Val Arg Ala Ser Gly Ile Lys Val Cys Cys Gly Gly Ile Leu Gly 180 185 190 atg ggt gaa acc aat gaa gat cgt att aaa atg ctg gtt ctg ctg gca 624 Met Gly Glu Thr Asn Glu Asp Arg Ile Lys Met Leu Val Leu Leu Ala 195 200 205 aat ctg aat gat ccg ccg gaa agc gtt ccg att aat acc ctg att aaa 672 Asn Leu Asn Asp Pro Pro Glu Ser Val Pro Ile Asn Thr Leu Ile Lys 210 215 220 att ccg ggt acc ccg ctg gaa aat gtt gca gat gtt gat ccg ttt gat 720 Ile Pro Gly Thr Pro Leu Glu Asn Val Ala Asp Val Asp Pro Phe Asp 225 230 235 240 ttt gtt cgt acc att gca att gcc cgt att att atg ccg aaa agc tat 768 Phe Val Arg Thr Ile Ala Ile Ala Arg Ile Ile Met Pro Lys Ser Tyr 245 250 255 att cgt ctg agc gca ggt cgt gaa aaa atg agc gat gaa ctg cag gca 816 Ile Arg Leu Ser Ala Gly Arg Glu Lys Met Ser Asp Glu Leu Gln Ala 260 265 270 ctg tgt ttt ctg gcc ggt gca aat agc att ttt tat ggt gaa aaa ctg 864 Leu Cys Phe Leu Ala Gly Ala Asn Ser Ile Phe Tyr Gly Glu Lys Leu 275 280 285 ctg acc gca cag aat ccg att ccg gaa cag gat aat cat ctg ttt cag 912 Leu Thr Ala Gln Asn Pro Ile Pro Glu Gln Asp Asn His Leu Phe Gln 290 295 300 cgt ctg ggc ctg cag aaa ctg gca ctg ctg cgt gaa aat taa 954 Arg Leu Gly Leu Gln Lys Leu Ala Leu Leu Arg Glu Asn 305 310 315 <210> 41 <211> 317 <212> PRT <213> Wolbachia endosymbiont of Cimex lectularius <400> 41 Met Thr Glu Glu Trp Thr Phe Ala Lys Ala Asp Gln Ile Phe Asn Phe 1 5 10 15 Pro Phe Pro Glu Leu Ile Tyr Ile Ala Gln Thr Glu His Arg Lys Gln 20 25 30 Phe Asn Pro Ser Glu Val Gln Ile Ser Thr Leu Leu Ser Ile Lys Thr 35 40 45 Gly Ser Cys Pro Glu Asn Cys Ser Tyr Cys Pro Gln Ser Ala His Tyr 50 55 60 Asn Thr Gly Leu Gln Lys Lys Pro Leu Leu Glu Ile Ala Glu Val Ile 65 70 75 80 Glu Ala Ala Lys Cys Ala Lys Glu Ala Gly Ser Thr Arg Phe Cys Met 85 90 95 Gly Ala Ala Trp Arg Gly Pro Arg Asp Gln Asp Leu Lys Val Val Cys 100 105 110 Glu Met Ile Arg Glu Val Lys Lys Leu Gly Leu Glu Thr Cys Val Thr 115 120 125 Leu Gly Leu Leu Lys Asp His Gln Ala Asn Met Leu Lys Glu Ala Gly 130 135 140 Leu Asp Phe Tyr Asn His Asn Ile Asp Thr Ser Glu Glu Tyr Tyr Asn 145 150 155 160 Lys Val Ile Thr Thr Arg Thr Phe Gln Asp Arg Leu Glu Thr Leu Glu 165 170 175 Cys Val Arg Ala Ser Gly Ile Lys Val Cys Cys Gly Gly Ile Leu Gly 180 185 190 Met Gly Glu Thr Asn Glu Asp Arg Ile Lys Met Leu Val Leu Leu Ala 195 200 205 Asn Leu Asn Asp Pro Pro Glu Ser Val Pro Ile Asn Thr Leu Ile Lys 210 215 220 Ile Pro Gly Thr Pro Leu Glu Asn Val Ala Asp Val Asp Pro Phe Asp 225 230 235 240 Phe Val Arg Thr Ile Ala Ile Ala Arg Ile Ile Met Pro Lys Ser Tyr 245 250 255 Ile Arg Leu Ser Ala Gly Arg Glu Lys Met Ser Asp Glu Leu Gln Ala 260 265 270 Leu Cys Phe Leu Ala Gly Ala Asn Ser Ile Phe Tyr Gly Glu Lys Leu 275 280 285 Leu Thr Ala Gln Asn Pro Ile Pro Glu Gln Asp Asn His Leu Phe Gln 290 295 300 Arg Leu Gly Leu Gln Lys Leu Ala Leu Leu Arg Glu Asn 305 310 315 <210> 42 <211> 1026 <212> DNA <213> Sphingomonas paucimobilis <220> <221> CDS <222> (1)..(1026) <223> bioB gene encoding biotin synthase from Sphingomonas paucimobilis NBRC 13935 <400> 42 atg acc acc acc ccg gca ctg agc agt gaa gca acc ccg cgt acc gat 48 Met Thr Thr Thr Pro Ala Leu Ser Ser Glu Ala Thr Pro Arg Thr Asp 1 5 10 15 tgg acc cgt gca gaa att gca gca ctg ttt gat ctg ccg ttt acc gaa 96 Trp Thr Arg Ala Glu Ile Ala Ala Leu Phe Asp Leu Pro Phe Thr Glu 20 25 30 ctg ctg ttt cgt gcc gca gaa gtt cat cgt gca cat cat gca gca gat 144 Leu Leu Phe Arg Ala Ala Glu Val His Arg Ala His His Ala Ala Asp 35 40 45 cag gtt cag ctg agc acc ctg ctg agc att aaa acc ggt ggt tgt ccg 192 Gln Val Gln Leu Ser Thr Leu Leu Ser Ile Lys Thr Gly Gly Cys Pro 50 55 60 gaa gat tgt ggt tat tgt agc cag agc acc cat gca gat acc ggt ctg 240 Glu Asp Cys Gly Tyr Cys Ser Gln Ser Thr His Ala Asp Thr Gly Leu 65 70 75 80 aaa gca acc aaa ctg atg gat ccg cgt gca gtt ctg cag gca gca gca 288 Lys Ala Thr Lys Leu Met Asp Pro Arg Ala Val Leu Gln Ala Ala Ala 85 90 95 cag gca aaa gat cat ggt agc acc cgt ttt tgt atg ggt gca gca tgg 336 Gln Ala Lys Asp His Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp 100 105 110 cgt aat ccg aaa gat cgt gat atg ccg gca att gtt gaa atg gtg aaa 384 Arg Asn Pro Lys Asp Arg Asp Met Pro Ala Ile Val Glu Met Val Lys 115 120 125 ggt gtt cgt gca atg ggt atg gaa acc tgt atg acc ctg ggt atg ctg 432 Gly Val Arg Ala Met Gly Met Glu Thr Cys Met Thr Leu Gly Met Leu 130 135 140 acc gat gca cag gca cag acc ctg gca gaa gca ggt ctg gat tat tat 480 Thr Asp Ala Gln Ala Gln Thr Leu Ala Glu Ala Gly Leu Asp Tyr Tyr 145 150 155 160 aat cat aat att gat acc agc ccg gaa cgt tat ggt gat gtg att acc 528 Asn His Asn Ile Asp Thr Ser Pro Glu Arg Tyr Gly Asp Val Ile Thr 165 170 175 acc cgt agc ttt ggt gaa cgt ctg gaa acc ctg gaa cat gtt cgt gat 576 Thr Arg Ser Phe Gly Glu Arg Leu Glu Thr Leu Glu His Val Arg Asp 180 185 190 gca ggt att aat gtt tgt tgt ggt ggt att gtt ggt atg ggt gaa acc 624 Ala Gly Ile Asn Val Cys Cys Gly Gly Ile Val Gly Met Gly Glu Thr 195 200 205 cgt ggt gat cgt gtt ggt ttt att cat gca ctg gca acc ctg ccg gtt 672 Arg Gly Asp Arg Val Gly Phe Ile His Ala Leu Ala Thr Leu Pro Val 210 215 220 cat ccg ggt agt gtg ccg gtt aat gca ctg gtt ccg gtt aaa ggt acc 720 His Pro Gly Ser Val Pro Val Asn Ala Leu Val Pro Val Lys Gly Thr 225 230 235 240 gtt ctg ggt gat atg ctg gca gat acc ccg ctg gca aaa att gat gat 768 Val Leu Gly Asp Met Leu Ala Asp Thr Pro Leu Ala Lys Ile Asp Asp 245 250 255 att gaa ttt gtt cgt acc gtt gca gtt gca cgt att acc atg ccg cat 816 Ile Glu Phe Val Arg Thr Val Ala Val Ala Arg Ile Thr Met Pro His 260 265 270 agc atg gtt cgt ctg agc gca ggt cgt gaa agc atg agc gat gca acc 864 Ser Met Val Arg Leu Ser Ala Gly Arg Glu Ser Met Ser Asp Ala Thr 275 280 285 cag gca ctg tgt ttt ctg gcc ggt gca aat agc att ttt acc ggt gat 912 Gln Ala Leu Cys Phe Leu Ala Gly Ala Asn Ser Ile Phe Thr Gly Asp 290 295 300 aaa ctg ctg acc gca ggt aat gca ggc gat gat aaa gat gca gcc ctg 960 Lys Leu Leu Thr Ala Gly Asn Ala Gly Asp Asp Lys Asp Ala Ala Leu 305 310 315 320 ttt gca cgt ctg ggt ctg acc ccg atg gca gca gaa tgt aaa gtt gaa 1008 Phe Ala Arg Leu Gly Leu Thr Pro Met Ala Ala Glu Cys Lys Val Glu 325 330 335 ctg gaa gca gca gaa taa 1026 Leu Glu Ala Ala Glu 340 <210> 43 <211> 341 <212> PRT <213> Sphingomonas paucimobilis <400> 43 Met Thr Thr Thr Pro Ala Leu Ser Ser Glu Ala Thr Pro Arg Thr Asp 1 5 10 15 Trp Thr Arg Ala Glu Ile Ala Ala Leu Phe Asp Leu Pro Phe Thr Glu 20 25 30 Leu Leu Phe Arg Ala Ala Glu Val His Arg Ala His His Ala Ala Asp 35 40 45 Gln Val Gln Leu Ser Thr Leu Leu Ser Ile Lys Thr Gly Gly Cys Pro 50 55 60 Glu Asp Cys Gly Tyr Cys Ser Gln Ser Thr His Ala Asp Thr Gly Leu 65 70 75 80 Lys Ala Thr Lys Leu Met Asp Pro Arg Ala Val Leu Gln Ala Ala Ala 85 90 95 Gln Ala Lys Asp His Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp 100 105 110 Arg Asn Pro Lys Asp Arg Asp Met Pro Ala Ile Val Glu Met Val Lys 115 120 125 Gly Val Arg Ala Met Gly Met Glu Thr Cys Met Thr Leu Gly Met Leu 130 135 140 Thr Asp Ala Gln Ala Gln Thr Leu Ala Glu Ala Gly Leu Asp Tyr Tyr 145 150 155 160 Asn His Asn Ile Asp Thr Ser Pro Glu Arg Tyr Gly Asp Val Ile Thr 165 170 175 Thr Arg Ser Phe Gly Glu Arg Leu Glu Thr Leu Glu His Val Arg Asp 180 185 190 Ala Gly Ile Asn Val Cys Cys Gly Gly Ile Val Gly Met Gly Glu Thr 195 200 205 Arg Gly Asp Arg Val Gly Phe Ile His Ala Leu Ala Thr Leu Pro Val 210 215 220 His Pro Gly Ser Val Pro Val Asn Ala Leu Val Pro Val Lys Gly Thr 225 230 235 240 Val Leu Gly Asp Met Leu Ala Asp Thr Pro Leu Ala Lys Ile Asp Asp 245 250 255 Ile Glu Phe Val Arg Thr Val Ala Val Ala Arg Ile Thr Met Pro His 260 265 270 Ser Met Val Arg Leu Ser Ala Gly Arg Glu Ser Met Ser Asp Ala Thr 275 280 285 Gln Ala Leu Cys Phe Leu Ala Gly Ala Asn Ser Ile Phe Thr Gly Asp 290 295 300 Lys Leu Leu Thr Ala Gly Asn Ala Gly Asp Asp Lys Asp Ala Ala Leu 305 310 315 320 Phe Ala Arg Leu Gly Leu Thr Pro Met Ala Ala Glu Cys Lys Val Glu 325 330 335 Leu Glu Ala Ala Glu 340 <210> 44 <211> 951 <212> DNA <213> Acidithiobacillus ferrivorans <220> <221> CDS <222> (1)..(951) <223> bioB gene encoding biotin synthase from Acidithiobacillus ferrivorans SS3 <400> 44 atg aat acc acc gca ccg ccg cag acc ctg gat gca att ctg gaa att 48 Met Asn Thr Thr Ala Pro Pro Gln Thr Leu Asp Ala Ile Leu Glu Ile 1 5 10 15 tat gcc agc ccg ttt aat gat ctg att ttt gaa gca cag aaa gtg cat 96 Tyr Ala Ser Pro Phe Asn Asp Leu Ile Phe Glu Ala Gln Lys Val His 20 25 30 cgc ctg cat ttt gat ccg aat gcc att cag tgt agc acc ctg ctg agc 144 Arg Leu His Phe Asp Pro Asn Ala Ile Gln Cys Ser Thr Leu Leu Ser 35 40 45 att aaa acc ggt ggt tgt ccg gaa gat tgt ggt tat tgt agc cag agc 192 Ile Lys Thr Gly Gly Cys Pro Glu Asp Cys Gly Tyr Cys Ser Gln Ser 50 55 60 gca cat cat cag acc gca ctg aaa gca gaa gca ctg atg gat ctg gaa 240 Ala His His Gln Thr Ala Leu Lys Ala Glu Ala Leu Met Asp Leu Glu 65 70 75 80 cag gtt cgt gca gca gca cag gaa gca aaa gca aat ggt gca cag cgt 288 Gln Val Arg Ala Ala Ala Gln Glu Ala Lys Ala Asn Gly Ala Gln Arg 85 90 95 ctg tgt atg ggt gca gca tgg cgt agc ccg cat gat aaa gat att gaa 336 Leu Cys Met Gly Ala Ala Trp Arg Ser Pro His Asp Lys Asp Ile Glu 100 105 110 aaa gtt gca gca atg att ggt gtt gtt aaa gaa tat ggt ctg gaa agc 384 Lys Val Ala Ala Met Ile Gly Val Val Lys Glu Tyr Gly Leu Glu Ser 115 120 125 tgt gtt acc ctg ggt atg ctg aaa ccg ggt cag gca gaa cgt ctg cag 432 Cys Val Thr Leu Gly Met Leu Lys Pro Gly Gln Ala Glu Arg Leu Gln 130 135 140 aat gcg ggt ctg gat tat tat aat cat aat ctg gat acc agc ccg gaa 480 Asn Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu Asp Thr Ser Pro Glu 145 150 155 160 ttt tat ggt gaa gtt att cat acc cgt agc tat cag gat cgt ctg gat 528 Phe Tyr Gly Glu Val Ile His Thr Arg Ser Tyr Gln Asp Arg Leu Asp 165 170 175 acc ctg gaa acc gtt cgt agc gca ggt att aaa att tgt agc ggt ggc 576 Thr Leu Glu Thr Val Arg Ser Ala Gly Ile Lys Ile Cys Ser Gly Gly 180 185 190 att ctg ggt atg ggt gaa agc cgt cgt gat cgt gca cgt atg ctg cag 624 Ile Leu Gly Met Gly Glu Ser Arg Arg Asp Arg Ala Arg Met Leu Gln 195 200 205 att ctg gca cat ctg ccg cag gca ccg gaa agc att ccg att aat gca 672 Ile Leu Ala His Leu Pro Gln Ala Pro Glu Ser Ile Pro Ile Asn Ala 210 215 220 ctg gtt ccg gtt ccg ggt acc ccg ctg gaa gca gca gaa ccg att gat 720 Leu Val Pro Val Pro Gly Thr Pro Leu Glu Ala Ala Glu Pro Ile Asp 225 230 235 240 ggt ttt gaa ttt gtt cgt acc att gca gtt gca cgt att ctg ttt ccg 768 Gly Phe Glu Phe Val Arg Thr Ile Ala Val Ala Arg Ile Leu Phe Pro 245 250 255 aaa gca tat gtt cgt ctg agc gca ggt cgt ggt gca atg agc gat gaa 816 Lys Ala Tyr Val Arg Leu Ser Ala Gly Arg Gly Ala Met Ser Asp Glu 260 265 270 ctg cag gca ctg gca ttt ctg gcc ggt gca aat agc att ttt ctg ggt 864 Leu Gln Ala Leu Ala Phe Leu Ala Gly Ala Asn Ser Ile Phe Leu Gly 275 280 285 gat cgt ctg ctg acc acc gat aat gca agc atg ggt cat gat cag agc 912 Asp Arg Leu Leu Thr Thr Asp Asn Ala Ser Met Gly His Asp Gln Ser 290 295 300 ctg ttt agc cgt ctg ggt ctg cat cgt agc gaa gca taa 951 Leu Phe Ser Arg Leu Gly Leu His Arg Ser Glu Ala 305 310 315 <210> 45 <211> 316 <212> PRT <213> Acidithiobacillus ferrivorans <400> 45 Met Asn Thr Thr Ala Pro Pro Gln Thr Leu Asp Ala Ile Leu Glu Ile 1 5 10 15 Tyr Ala Ser Pro Phe Asn Asp Leu Ile Phe Glu Ala Gln Lys Val His 20 25 30 Arg Leu His Phe Asp Pro Asn Ala Ile Gln Cys Ser Thr Leu Leu Ser 35 40 45 Ile Lys Thr Gly Gly Cys Pro Glu Asp Cys Gly Tyr Cys Ser Gln Ser 50 55 60 Ala His His Gln Thr Ala Leu Lys Ala Glu Ala Leu Met Asp Leu Glu 65 70 75 80 Gln Val Arg Ala Ala Ala Gln Glu Ala Lys Ala Asn Gly Ala Gln Arg 85 90 95 Leu Cys Met Gly Ala Ala Trp Arg Ser Pro His Asp Lys Asp Ile Glu 100 105 110 Lys Val Ala Ala Met Ile Gly Val Val Lys Glu Tyr Gly Leu Glu Ser 115 120 125 Cys Val Thr Leu Gly Met Leu Lys Pro Gly Gln Ala Glu Arg Leu Gln 130 135 140 Asn Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu Asp Thr Ser Pro Glu 145 150 155 160 Phe Tyr Gly Glu Val Ile His Thr Arg Ser Tyr Gln Asp Arg Leu Asp 165 170 175 Thr Leu Glu Thr Val Arg Ser Ala Gly Ile Lys Ile Cys Ser Gly Gly 180 185 190 Ile Leu Gly Met Gly Glu Ser Arg Arg Asp Arg Ala Arg Met Leu Gln 195 200 205 Ile Leu Ala His Leu Pro Gln Ala Pro Glu Ser Ile Pro Ile Asn Ala 210 215 220 Leu Val Pro Val Pro Gly Thr Pro Leu Glu Ala Ala Glu Pro Ile Asp 225 230 235 240 Gly Phe Glu Phe Val Arg Thr Ile Ala Val Ala Arg Ile Leu Phe Pro 245 250 255 Lys Ala Tyr Val Arg Leu Ser Ala Gly Arg Gly Ala Met Ser Asp Glu 260 265 270 Leu Gln Ala Leu Ala Phe Leu Ala Gly Ala Asn Ser Ile Phe Leu Gly 275 280 285 Asp Arg Leu Leu Thr Thr Asp Asn Ala Ser Met Gly His Asp Gln Ser 290 295 300 Leu Phe Ser Arg Leu Gly Leu His Arg Ser Glu Ala 305 310 315 <210> 46 <211> 996 <212> DNA <213> Gallionella capsiferriformans <220> <221> CDS <222> (1)..(996) <223> bioB gene encoding biotin synthase from Gallionella capsiferriformans ES-2 <400> 46 atg aat acc cag acc att gcc ttt cat cat ccg gtt aaa cgt acc gca 48 Met Asn Thr Gln Thr Ile Ala Phe His His Pro Val Lys Arg Thr Ala 1 5 10 15 acc ccg gaa cgt tgg agc gtt gaa gca gtt gaa agc ctg ttt gca ctg 96 Thr Pro Glu Arg Trp Ser Val Glu Ala Val Glu Ser Leu Phe Ala Leu 20 25 30 ccg ttt gcc gat ctg ctg tat cgt gca cag cag gtt cat cgt gaa cat 144 Pro Phe Ala Asp Leu Leu Tyr Arg Ala Gln Gln Val His Arg Glu His 35 40 45 ttt gat ccg aat cag gtt cag ctg agc acc ctg ctg agc att aaa acc 192 Phe Asp Pro Asn Gln Val Gln Leu Ser Thr Leu Leu Ser Ile Lys Thr 50 55 60 ggt ggt tgt agc gaa gat tgt ggt tat tgt ccg cag agc gca ttt cat 240 Gly Gly Cys Ser Glu Asp Cys Gly Tyr Cys Pro Gln Ser Ala Phe His 65 70 75 80 agc acc ggt gtt gaa gat cgt aaa atg ctg gca ctg gat gca gtt att 288 Ser Thr Gly Val Glu Asp Arg Lys Met Leu Ala Leu Asp Ala Val Ile 85 90 95 gaa gca gca aaa gca gca cag gca gca ggc gca gat cgt ttt tgt atg 336 Glu Ala Ala Lys Ala Ala Gln Ala Ala Gly Ala Asp Arg Phe Cys Met 100 105 110 ggt gca gca tgg cgt gaa ccg agc gaa gca gat atg ctg agc gtt gtt 384 Gly Ala Ala Trp Arg Glu Pro Ser Glu Ala Asp Met Leu Ser Val Val 115 120 125 gat atg gtt cag gca gtt cgt ggc ctg ggt atg gaa acc tgt gca acc 432 Asp Met Val Gln Ala Val Arg Gly Leu Gly Met Glu Thr Cys Ala Thr 130 135 140 ctg ggt atg ctg aat gat gca cag acc gaa cag ctg cgt gca gcc ggt 480 Leu Gly Met Leu Asn Asp Ala Gln Thr Glu Gln Leu Arg Ala Ala Gly 145 150 155 160 ctg gat tat tat aat cat aat ctg gat acc agc ccg gaa ttt tat ggc 528 Leu Asp Tyr Tyr Asn His Asn Leu Asp Thr Ser Pro Glu Phe Tyr Gly 165 170 175 gat att att agc acc cgt gat tat cag gat cgc ctg gat acc ctg gaa 576 Asp Ile Ile Ser Thr Arg Asp Tyr Gln Asp Arg Leu Asp Thr Leu Glu 180 185 190 cgc gtt cgt cgt gca ggt atg cat gtt tgt agc ggt ggt att gtt ggt 624 Arg Val Arg Arg Ala Gly Met His Val Cys Ser Gly Gly Ile Val Gly 195 200 205 atg ggt gaa agt ctg acc gaa cgt gca ggt ctg gtt gcc cag ctg gca 672 Met Gly Glu Ser Leu Thr Glu Arg Ala Gly Leu Val Ala Gln Leu Ala 210 215 220 aat ctg aat ccg tat ccg gaa agc gtt ccg att aat aat ctg gtt aaa 720 Asn Leu Asn Pro Tyr Pro Glu Ser Val Pro Ile Asn Asn Leu Val Lys 225 230 235 240 gtt gaa ggt acc ccg ctg gca gat gca gca gaa ctg gat ccg ctg gat 768 Val Glu Gly Thr Pro Leu Ala Asp Ala Ala Glu Leu Asp Pro Leu Asp 245 250 255 ttt gtt cgt acc att gca gtt gca cgt att acc atg ccg acc gca cgt 816 Phe Val Arg Thr Ile Ala Val Ala Arg Ile Thr Met Pro Thr Ala Arg 260 265 270 gtt cgt ctg agc gca ggt cgt cag gca atg agc gat gca att cag gca 864 Val Arg Leu Ser Ala Gly Arg Gln Ala Met Ser Asp Ala Ile Gln Ala 275 280 285 ctg tgt ttt ctg gcc ggt gca aat agc att ttt tat ggt gaa cag ctg 912 Leu Cys Phe Leu Ala Gly Ala Asn Ser Ile Phe Tyr Gly Glu Gln Leu 290 295 300 ctg acc acc ggt aat ccg gaa gtt gaa cgt gat cgt gca ctg atg gat 960 Leu Thr Thr Gly Asn Pro Glu Val Glu Arg Asp Arg Ala Leu Met Asp 305 310 315 320 aaa ctg ggt atg tat ccg ttt gca gat aaa cat taa 996 Lys Leu Gly Met Tyr Pro Phe Ala Asp Lys His 325 330 <210> 47 <211> 331 <212> PRT <213> Gallionella capsiferriformans <400> 47 Met Asn Thr Gln Thr Ile Ala Phe His His Pro Val Lys Arg Thr Ala 1 5 10 15 Thr Pro Glu Arg Trp Ser Val Glu Ala Val Glu Ser Leu Phe Ala Leu 20 25 30 Pro Phe Ala Asp Leu Leu Tyr Arg Ala Gln Gln Val His Arg Glu His 35 40 45 Phe Asp Pro Asn Gln Val Gln Leu Ser Thr Leu Leu Ser Ile Lys Thr 50 55 60 Gly Gly Cys Ser Glu Asp Cys Gly Tyr Cys Pro Gln Ser Ala Phe His 65 70 75 80 Ser Thr Gly Val Glu Asp Arg Lys Met Leu Ala Leu Asp Ala Val Ile 85 90 95 Glu Ala Ala Lys Ala Ala Gln Ala Ala Gly Ala Asp Arg Phe Cys Met 100 105 110 Gly Ala Ala Trp Arg Glu Pro Ser Glu Ala Asp Met Leu Ser Val Val 115 120 125 Asp Met Val Gln Ala Val Arg Gly Leu Gly Met Glu Thr Cys Ala Thr 130 135 140 Leu Gly Met Leu Asn Asp Ala Gln Thr Glu Gln Leu Arg Ala Ala Gly 145 150 155 160 Leu Asp Tyr Tyr Asn His Asn Leu Asp Thr Ser Pro Glu Phe Tyr Gly 165 170 175 Asp Ile Ile Ser Thr Arg Asp Tyr Gln Asp Arg Leu Asp Thr Leu Glu 180 185 190 Arg Val Arg Arg Ala Gly Met His Val Cys Ser Gly Gly Ile Val Gly 195 200 205 Met Gly Glu Ser Leu Thr Glu Arg Ala Gly Leu Val Ala Gln Leu Ala 210 215 220 Asn Leu Asn Pro Tyr Pro Glu Ser Val Pro Ile Asn Asn Leu Val Lys 225 230 235 240 Val Glu Gly Thr Pro Leu Ala Asp Ala Ala Glu Leu Asp Pro Leu Asp 245 250 255 Phe Val Arg Thr Ile Ala Val Ala Arg Ile Thr Met Pro Thr Ala Arg 260 265 270 Val Arg Leu Ser Ala Gly Arg Gln Ala Met Ser Asp Ala Ile Gln Ala 275 280 285 Leu Cys Phe Leu Ala Gly Ala Asn Ser Ile Phe Tyr Gly Glu Gln Leu 290 295 300 Leu Thr Thr Gly Asn Pro Glu Val Glu Arg Asp Arg Ala Leu Met Asp 305 310 315 320 Lys Leu Gly Met Tyr Pro Phe Ala Asp Lys His 325 330 <210> 48 <211> 1029 <212> DNA <213> Ralstonia eutropha <220> <221> CDS <222> (1)..(1029) <223> bioB gene encoding biotin synthase from Ralstonia eutropha JMP134 <400> 48 atg aat cag gca gca cag acc gtt gca acc att agc gca gaa gcc ctg 48 Met Asn Gln Ala Ala Gln Thr Val Ala Thr Ile Ser Ala Glu Ala Leu 1 5 10 15 cgt cag acc gca cgt aat acc cat gca ctg ccg gaa gat gca cgt tgg 96 Arg Gln Thr Ala Arg Asn Thr His Ala Leu Pro Glu Asp Ala Arg Trp 20 25 30 cgt gtt gat gat gtt gca gca ctg ttt gcc ctg ccg ttt aat gat ctg 144 Arg Val Asp Asp Val Ala Ala Leu Phe Ala Leu Pro Phe Asn Asp Leu 35 40 45 ctg ttt cgt gca cag cag gtt cat cgt gaa aat ttt gat gca aat acc 192 Leu Phe Arg Ala Gln Gln Val His Arg Glu Asn Phe Asp Ala Asn Thr 50 55 60 gtt cag ctg agc acc ctg ctg agc att aaa acc ggt ggt tgt gaa gaa 240 Val Gln Leu Ser Thr Leu Leu Ser Ile Lys Thr Gly Gly Cys Glu Glu 65 70 75 80 gat tgt ggt tat tgt ccg cag agc gca cat cat gat gcg ggt gtt aaa 288 Asp Cys Gly Tyr Cys Pro Gln Ser Ala His His Asp Ala Gly Val Lys 85 90 95 gcc gaa aaa ctg atg gaa ctg gat gaa gtt ctg gaa gca gca cgt gca 336 Ala Glu Lys Leu Met Glu Leu Asp Glu Val Leu Glu Ala Ala Arg Ala 100 105 110 gca aaa gca aat ggt gca acc cgt ttt tgt atg ggt gca gca tgg cgt 384 Ala Lys Ala Asn Gly Ala Thr Arg Phe Cys Met Gly Ala Ala Trp Arg 115 120 125 agc ccg aaa gat cgt cat ctg gaa ccg gtt atg gat atg gtt cgt gaa 432 Ser Pro Lys Asp Arg His Leu Glu Pro Val Met Asp Met Val Arg Glu 130 135 140 gtt aaa gca atg ggt ctg gaa acc tgt gtt acc ctg ggt atg ctg aaa 480 Val Lys Ala Met Gly Leu Glu Thr Cys Val Thr Leu Gly Met Leu Lys 145 150 155 160 gca gaa cag gcc cag cag ctg aaa gat gcc ggt ctg gat tat tat aat 528 Ala Glu Gln Ala Gln Gln Leu Lys Asp Ala Gly Leu Asp Tyr Tyr Asn 165 170 175 cat aat ctg gat acc agc ccg gaa ttt tat ggc aaa att att acc acc 576 His Asn Leu Asp Thr Ser Pro Glu Phe Tyr Gly Lys Ile Ile Thr Thr 180 185 190 cgt acc tat cag gat cgc ctg gat acc att ggc cat gtt cgt gat gca 624 Arg Thr Tyr Gln Asp Arg Leu Asp Thr Ile Gly His Val Arg Asp Ala 195 200 205 ggt att aat gtt tgt tgt ggt ggt att gtt ggt atg ggt gaa agc cgt 672 Gly Ile Asn Val Cys Cys Gly Gly Ile Val Gly Met Gly Glu Ser Arg 210 215 220 gaa gcc cgt gca ggt ctg att gca cag ctg gca aat atg gat ccg tat 720 Glu Ala Arg Ala Gly Leu Ile Ala Gln Leu Ala Asn Met Asp Pro Tyr 225 230 235 240 ccg gaa agc gtt ccg att aat aat ctg gtt cag gtt gaa ggt acc ccg 768 Pro Glu Ser Val Pro Ile Asn Asn Leu Val Gln Val Glu Gly Thr Pro 245 250 255 ctg gca ggt acc gaa gca ctg gat ccg ttt gaa ttt gtt cgt acc att 816 Leu Ala Gly Thr Glu Ala Leu Asp Pro Phe Glu Phe Val Arg Thr Ile 260 265 270 gca gtt gca cgt att acc atg ccg ggt gca atg gtt cgt ctg agc gca 864 Ala Val Ala Arg Ile Thr Met Pro Gly Ala Met Val Arg Leu Ser Ala 275 280 285 ggt cgt gaa gca atg gat gaa gca ctg cag gca ctg tgt ttt atg gcc 912 Gly Arg Glu Ala Met Asp Glu Ala Leu Gln Ala Leu Cys Phe Met Ala 290 295 300 ggt gca aat agc att ttt tat ggt gaa aaa ctg ctg acc acc ggt aat 960 Gly Ala Asn Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Gly Asn 305 310 315 320 ccg cag gca gat cgt gat cgt gca ctg ctg gca cgt ctg gat att cgt 1008 Pro Gln Ala Asp Arg Asp Arg Ala Leu Leu Ala Arg Leu Asp Ile Arg 325 330 335 gca gaa ggt tat gca ggt taa 1029 Ala Glu Gly Tyr Ala Gly 340 <210> 49 <211> 342 <212> PRT <213> Ralstonia eutropha <400> 49 Met Asn Gln Ala Ala Gln Thr Val Ala Thr Ile Ser Ala Glu Ala Leu 1 5 10 15 Arg Gln Thr Ala Arg Asn Thr His Ala Leu Pro Glu Asp Ala Arg Trp 20 25 30 Arg Val Asp Asp Val Ala Ala Leu Phe Ala Leu Pro Phe Asn Asp Leu 35 40 45 Leu Phe Arg Ala Gln Gln Val His Arg Glu Asn Phe Asp Ala Asn Thr 50 55 60 Val Gln Leu Ser Thr Leu Leu Ser Ile Lys Thr Gly Gly Cys Glu Glu 65 70 75 80 Asp Cys Gly Tyr Cys Pro Gln Ser Ala His His Asp Ala Gly Val Lys 85 90 95 Ala Glu Lys Leu Met Glu Leu Asp Glu Val Leu Glu Ala Ala Arg Ala 100 105 110 Ala Lys Ala Asn Gly Ala Thr Arg Phe Cys Met Gly Ala Ala Trp Arg 115 120 125 Ser Pro Lys Asp Arg His Leu Glu Pro Val Met Asp Met Val Arg Glu 130 135 140 Val Lys Ala Met Gly Leu Glu Thr Cys Val Thr Leu Gly Met Leu Lys 145 150 155 160 Ala Glu Gln Ala Gln Gln Leu Lys Asp Ala Gly Leu Asp Tyr Tyr Asn 165 170 175 His Asn Leu Asp Thr Ser Pro Glu Phe Tyr Gly Lys Ile Ile Thr Thr 180 185 190 Arg Thr Tyr Gln Asp Arg Leu Asp Thr Ile Gly His Val Arg Asp Ala 195 200 205 Gly Ile Asn Val Cys Cys Gly Gly Ile Val Gly Met Gly Glu Ser Arg 210 215 220 Glu Ala Arg Ala Gly Leu Ile Ala Gln Leu Ala Asn Met Asp Pro Tyr 225 230 235 240 Pro Glu Ser Val Pro Ile Asn Asn Leu Val Gln Val Glu Gly Thr Pro 245 250 255 Leu Ala Gly Thr Glu Ala Leu Asp Pro Phe Glu Phe Val Arg Thr Ile 260 265 270 Ala Val Ala Arg Ile Thr Met Pro Gly Ala Met Val Arg Leu Ser Ala 275 280 285 Gly Arg Glu Ala Met Asp Glu Ala Leu Gln Ala Leu Cys Phe Met Ala 290 295 300 Gly Ala Asn Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Gly Asn 305 310 315 320 Pro Gln Ala Asp Arg Asp Arg Ala Leu Leu Ala Arg Leu Asp Ile Arg 325 330 335 Ala Glu Gly Tyr Ala Gly 340 <210> 50 <211> 1008 <212> DNA <213> Bordetella parapertussis <220> <221> CDS <222> (1)..(1008) <223> bioB gene encoding biotin synthase from Bordetella parapertussis 12822 <400> 50 atg cat acc gca tat att ccg gtt ccg aca ccg gtt cgt ccg ccg agt 48 Met His Thr Ala Tyr Ile Pro Val Pro Thr Pro Val Arg Pro Pro Ser 1 5 10 15 gca gaa cgt tgg ccg ctg gca gca gtt gca gaa ctg ttt gaa ctg ccg 96 Ala Glu Arg Trp Pro Leu Ala Ala Val Ala Glu Leu Phe Glu Leu Pro 20 25 30 ttt ctg gat ctg ctg cat cgt gca cag cag gtt cat cgt cag cat ttt 144 Phe Leu Asp Leu Leu His Arg Ala Gln Gln Val His Arg Gln His Phe 35 40 45 gat gca aat acc gtt cag ctg agc agc ctg ctg agc att aaa acc ggt 192 Asp Ala Asn Thr Val Gln Leu Ser Ser Leu Leu Ser Ile Lys Thr Gly 50 55 60 ggt tgt ccg gaa gat tgt gca tat tgt ccg cag agc gca cat tat gat 240 Gly Cys Pro Glu Asp Cys Ala Tyr Cys Pro Gln Ser Ala His Tyr Asp 65 70 75 80 acc ggt gtt gat gca gat aaa ctg atg ccg ctg gat gaa gtt gtt cgt 288 Thr Gly Val Asp Ala Asp Lys Leu Met Pro Leu Asp Glu Val Val Arg 85 90 95 gca gcc cgt gca gca cag gca aat ggt gca cag cgt ttt tgt atg ggt 336 Ala Ala Arg Ala Ala Gln Ala Asn Gly Ala Gln Arg Phe Cys Met Gly 100 105 110 gca gca tgg cgt agc ccg aaa ccg cat cat ctg gaa gca gtg gca gaa 384 Ala Ala Trp Arg Ser Pro Lys Pro His His Leu Glu Ala Val Ala Glu 115 120 125 atg att ggt gca gtt aaa gca ctg ggt atg gaa acc tgt gtt acc ctg 432 Met Ile Gly Ala Val Lys Ala Leu Gly Met Glu Thr Cys Val Thr Leu 130 135 140 ggt atg ctg cgt gat ggt cag gca gaa cag ctg aaa gca gca ggc ctg 480 Gly Met Leu Arg Asp Gly Gln Ala Glu Gln Leu Lys Ala Ala Gly Leu 145 150 155 160 gat tat tat aat cat aat ctg gat acc gca ccg gaa ttt tat ggt aaa 528 Asp Tyr Tyr Asn His Asn Leu Asp Thr Ala Pro Glu Phe Tyr Gly Lys 165 170 175 att att agc acc cgt acc tat cag gat cgt ctg gat acc ctg cag cag 576 Ile Ile Ser Thr Arg Thr Tyr Gln Asp Arg Leu Asp Thr Leu Gln Gln 180 185 190 gtt cgt gaa gca ggt att aat gtt tgt tgt ggt ggt att gtt ggt atg 624 Val Arg Glu Ala Gly Ile Asn Val Cys Cys Gly Gly Ile Val Gly Met 195 200 205 ggt gaa agc cgt cgt gat cgt gca ggt ctg gtt gca cag ctg gca aat 672 Gly Glu Ser Arg Arg Asp Arg Ala Gly Leu Val Ala Gln Leu Ala Asn 210 215 220 atg gaa ccg tat ccg gaa agc gtt ccg att aat aat ctg gtt cag gtg 720 Met Glu Pro Tyr Pro Glu Ser Val Pro Ile Asn Asn Leu Val Gln Val 225 230 235 240 gaa ggt acc ccg ctg gcg ggt gca gaa acc ctg gat ccg ttt gaa ttt 768 Glu Gly Thr Pro Leu Ala Gly Ala Glu Thr Leu Asp Pro Phe Glu Phe 245 250 255 att cgt acc att gca gtt gca cgt att acc atg ccg ctg gcc aaa gtt 816 Ile Arg Thr Ile Ala Val Ala Arg Ile Thr Met Pro Leu Ala Lys Val 260 265 270 cgt ctg agc gca ggt cgt gaa acc atg agc gat agc gaa cag gca ctg 864 Arg Leu Ser Ala Gly Arg Glu Thr Met Ser Asp Ser Glu Gln Ala Leu 275 280 285 tgt ttt atg gcc ggt gca aat agc att ttt tat ggt gat gtt ctg ctg 912 Cys Phe Met Ala Gly Ala Asn Ser Ile Phe Tyr Gly Asp Val Leu Leu 290 295 300 aca acc ggt aat ccg cag gtt gaa gca gat cgt cgt ctg ctg cag cgt 960 Thr Thr Gly Asn Pro Gln Val Glu Ala Asp Arg Arg Leu Leu Gln Arg 305 310 315 320 ctg ggt atg cgt gca gaa ggt ctg ccg tgt gca gcc ggt cag gca taa 1008 Leu Gly Met Arg Ala Glu Gly Leu Pro Cys Ala Ala Gly Gln Ala 325 330 335 <210> 51 <211> 335 <212> PRT <213> Bordetella parapertussis <400> 51 Met His Thr Ala Tyr Ile Pro Val Pro Thr Pro Val Arg Pro Pro Ser 1 5 10 15 Ala Glu Arg Trp Pro Leu Ala Ala Val Ala Glu Leu Phe Glu Leu Pro 20 25 30 Phe Leu Asp Leu Leu His Arg Ala Gln Gln Val His Arg Gln His Phe 35 40 45 Asp Ala Asn Thr Val Gln Leu Ser Ser Leu Leu Ser Ile Lys Thr Gly 50 55 60 Gly Cys Pro Glu Asp Cys Ala Tyr Cys Pro Gln Ser Ala His Tyr Asp 65 70 75 80 Thr Gly Val Asp Ala Asp Lys Leu Met Pro Leu Asp Glu Val Val Arg 85 90 95 Ala Ala Arg Ala Ala Gln Ala Asn Gly Ala Gln Arg Phe Cys Met Gly 100 105 110 Ala Ala Trp Arg Ser Pro Lys Pro His His Leu Glu Ala Val Ala Glu 115 120 125 Met Ile Gly Ala Val Lys Ala Leu Gly Met Glu Thr Cys Val Thr Leu 130 135 140 Gly Met Leu Arg Asp Gly Gln Ala Glu Gln Leu Lys Ala Ala Gly Leu 145 150 155 160 Asp Tyr Tyr Asn His Asn Leu Asp Thr Ala Pro Glu Phe Tyr Gly Lys 165 170 175 Ile Ile Ser Thr Arg Thr Tyr Gln Asp Arg Leu Asp Thr Leu Gln Gln 180 185 190 Val Arg Glu Ala Gly Ile Asn Val Cys Cys Gly Gly Ile Val Gly Met 195 200 205 Gly Glu Ser Arg Arg Asp Arg Ala Gly Leu Val Ala Gln Leu Ala Asn 210 215 220 Met Glu Pro Tyr Pro Glu Ser Val Pro Ile Asn Asn Leu Val Gln Val 225 230 235 240 Glu Gly Thr Pro Leu Ala Gly Ala Glu Thr Leu Asp Pro Phe Glu Phe 245 250 255 Ile Arg Thr Ile Ala Val Ala Arg Ile Thr Met Pro Leu Ala Lys Val 260 265 270 Arg Leu Ser Ala Gly Arg Glu Thr Met Ser Asp Ser Glu Gln Ala Leu 275 280 285 Cys Phe Met Ala Gly Ala Asn Ser Ile Phe Tyr Gly Asp Val Leu Leu 290 295 300 Thr Thr Gly Asn Pro Gln Val Glu Ala Asp Arg Arg Leu Leu Gln Arg 305 310 315 320 Leu Gly Met Arg Ala Glu Gly Leu Pro Cys Ala Ala Gly Gln Ala 325 330 335 <210> 52 <211> 1044 <212> DNA <213> Pusillimonas sp. T7-7 <220> <221> CDS <222> (1)..(1044) <223> bioB gene encoding biotin synthase from Pusillimonas sp. T7-7 <400> 52 atg gca gca atg aaa ccg gca att ccg agc cat acc ccg acc ccg gat 48 Met Ala Ala Met Lys Pro Ala Ile Pro Ser His Thr Pro Thr Pro Asp 1 5 10 15 cat gca ccg cag gca tgg ggt att gca cag att ctg cgt ctg tat gaa 96 His Ala Pro Gln Ala Trp Gly Ile Ala Gln Ile Leu Arg Leu Tyr Glu 20 25 30 ctg ccg ttt ctg gat ctg ctg cat cag gca cag gcc gtt cat cgt gca 144 Leu Pro Phe Leu Asp Leu Leu His Gln Ala Gln Ala Val His Arg Ala 35 40 45 cat cat cag ccg aat acc gtt cag ctg agc agc ctg ctg agc att aaa 192 His His Gln Pro Asn Thr Val Gln Leu Ser Ser Leu Leu Ser Ile Lys 50 55 60 acc ggt gca tgt ccg gaa gat tgt gca tat tgt ccg cag agc gca cgt 240 Thr Gly Ala Cys Pro Glu Asp Cys Ala Tyr Cys Pro Gln Ser Ala Arg 65 70 75 80 cat gat acc ggt ggt aaa cag gaa gca ctg atg ccg gtt gca gaa gtt 288 His Asp Thr Gly Gly Lys Gln Glu Ala Leu Met Pro Val Ala Glu Val 85 90 95 ctg gaa gca gca cgt aaa gca aaa gca aat ggt gca cag cgt ttt tgt 336 Leu Glu Ala Ala Arg Lys Ala Lys Ala Asn Gly Ala Gln Arg Phe Cys 100 105 110 atg ggt gca gca tgg cgt agc ccg acc gca cgt cag ctg gat agc gtt 384 Met Gly Ala Ala Trp Arg Ser Pro Thr Ala Arg Gln Leu Asp Ser Val 115 120 125 gtt gaa atg gtt ggt gca gtt aaa gca ctg ggt ctg gaa acc tgt gtt 432 Val Glu Met Val Gly Ala Val Lys Ala Leu Gly Leu Glu Thr Cys Val 130 135 140 acc ctg ggt atg ctg aaa gaa ggt cag gca gaa cgt ctg cgt gat gcg 480 Thr Leu Gly Met Leu Lys Glu Gly Gln Ala Glu Arg Leu Arg Asp Ala 145 150 155 160 ggt ctg gat tat tat aat cat aat ctg gat acc agc ccg gaa ttt tat 528 Gly Leu Asp Tyr Tyr Asn His Asn Leu Asp Thr Ser Pro Glu Phe Tyr 165 170 175 ggt aat att att acc acc cgt agc tat cag gat cgt ctg gat acc ctg 576 Gly Asn Ile Ile Thr Thr Arg Ser Tyr Gln Asp Arg Leu Asp Thr Leu 180 185 190 gaa cgt gtt cgt aat gcc ggt gtt cat gtt tgt tgt ggt ggt att gtt 624 Glu Arg Val Arg Asn Ala Gly Val His Val Cys Cys Gly Gly Ile Val 195 200 205 ggt ctg ggt gaa agc cgt aaa gaa cgt gca ggt ctg gtt gca cag ctg 672 Gly Leu Gly Glu Ser Arg Lys Glu Arg Ala Gly Leu Val Ala Gln Leu 210 215 220 gca aat ctg agc ccg tat ccg gaa agc gtt ccg gtt aat aat ctg gtt 720 Ala Asn Leu Ser Pro Tyr Pro Glu Ser Val Pro Val Asn Asn Leu Val 225 230 235 240 aaa gtt gca ggt acc ccg ctg gat gcc acc ccg gat att gat ccg ttt 768 Lys Val Ala Gly Thr Pro Leu Asp Ala Thr Pro Asp Ile Asp Pro Phe 245 250 255 gaa ttt gtt cgt acc att gca gtt gca cgt att acc atg ccg cgt gca 816 Glu Phe Val Arg Thr Ile Ala Val Ala Arg Ile Thr Met Pro Arg Ala 260 265 270 gtt gtt cgt ctg agc gca ggt cgt gaa gca atg agc gat gca att cag 864 Val Val Arg Leu Ser Ala Gly Arg Glu Ala Met Ser Asp Ala Ile Gln 275 280 285 gca ctg tgt ttt atg gcc ggt gca aat agc att ttt tat ggt gaa cag 912 Ala Leu Cys Phe Met Ala Gly Ala Asn Ser Ile Phe Tyr Gly Glu Gln 290 295 300 ctg ctg aca aca gca aat ccg cag ctg agt cag gat cag caa ctg ttt 960 Leu Leu Thr Thr Ala Asn Pro Gln Leu Ser Gln Asp Gln Gln Leu Phe 305 310 315 320 cag cgt ctg ggt ctg aca gca acc ccg gca gat ccg gca cgt ccg gca 1008 Gln Arg Leu Gly Leu Thr Ala Thr Pro Ala Asp Pro Ala Arg Pro Ala 325 330 335 cat ctg gaa cat cat cat gaa gca acc ctg gca taa 1044 His Leu Glu His His His Glu Ala Thr Leu Ala 340 345 <210> 53 <211> 347 <212> PRT <213> Pusillimonas sp. T7-7 <400> 53 Met Ala Ala Met Lys Pro Ala Ile Pro Ser His Thr Pro Thr Pro Asp 1 5 10 15 His Ala Pro Gln Ala Trp Gly Ile Ala Gln Ile Leu Arg Leu Tyr Glu 20 25 30 Leu Pro Phe Leu Asp Leu Leu His Gln Ala Gln Ala Val His Arg Ala 35 40 45 His His Gln Pro Asn Thr Val Gln Leu Ser Ser Leu Leu Ser Ile Lys 50 55 60 Thr Gly Ala Cys Pro Glu Asp Cys Ala Tyr Cys Pro Gln Ser Ala Arg 65 70 75 80 His Asp Thr Gly Gly Lys Gln Glu Ala Leu Met Pro Val Ala Glu Val 85 90 95 Leu Glu Ala Ala Arg Lys Ala Lys Ala Asn Gly Ala Gln Arg Phe Cys 100 105 110 Met Gly Ala Ala Trp Arg Ser Pro Thr Ala Arg Gln Leu Asp Ser Val 115 120 125 Val Glu Met Val Gly Ala Val Lys Ala Leu Gly Leu Glu Thr Cys Val 130 135 140 Thr Leu Gly Met Leu Lys Glu Gly Gln Ala Glu Arg Leu Arg Asp Ala 145 150 155 160 Gly Leu Asp Tyr Tyr Asn His Asn Leu Asp Thr Ser Pro Glu Phe Tyr 165 170 175 Gly Asn Ile Ile Thr Thr Arg Ser Tyr Gln Asp Arg Leu Asp Thr Leu 180 185 190 Glu Arg Val Arg Asn Ala Gly Val His Val Cys Cys Gly Gly Ile Val 195 200 205 Gly Leu Gly Glu Ser Arg Lys Glu Arg Ala Gly Leu Val Ala Gln Leu 210 215 220 Ala Asn Leu Ser Pro Tyr Pro Glu Ser Val Pro Val Asn Asn Leu Val 225 230 235 240 Lys Val Ala Gly Thr Pro Leu Asp Ala Thr Pro Asp Ile Asp Pro Phe 245 250 255 Glu Phe Val Arg Thr Ile Ala Val Ala Arg Ile Thr Met Pro Arg Ala 260 265 270 Val Val Arg Leu Ser Ala Gly Arg Glu Ala Met Ser Asp Ala Ile Gln 275 280 285 Ala Leu Cys Phe Met Ala Gly Ala Asn Ser Ile Phe Tyr Gly Glu Gln 290 295 300 Leu Leu Thr Thr Ala Asn Pro Gln Leu Ser Gln Asp Gln Gln Leu Phe 305 310 315 320 Gln Arg Leu Gly Leu Thr Ala Thr Pro Ala Asp Pro Ala Arg Pro Ala 325 330 335 His Leu Glu His His His Glu Ala Thr Leu Ala 340 345 <210> 54 <211> 957 <212> DNA <213> Cenarchaeum symbiosum A <220> <221> CDS <222> (1)..(957) <223> bioB gene encoding biotin synthase from Cenarchaeum symbiosum A <400> 54 atg ggt att gca gaa tgt cgt gat aaa gtt ctg ggt ggt ggt gaa ctg 48 Met Gly Ile Ala Glu Cys Arg Asp Lys Val Leu Gly Gly Gly Glu Leu 1 5 10 15 acc aaa gat gaa gca cgt ggt ctg atg gaa gca gat gtt acc gaa ctg 96 Thr Lys Asp Glu Ala Arg Gly Leu Met Glu Ala Asp Val Thr Glu Leu 20 25 30 gcc gca gca gca gat gaa att acc cgt cgt ttt aat ggt gat ggt gtg 144 Ala Ala Ala Ala Asp Glu Ile Thr Arg Arg Phe Asn Gly Asp Gly Val 35 40 45 gat gtg gaa cag ctg aat aat att aaa cgt gat ggt tgc agc gaa gat 192 Asp Val Glu Gln Leu Asn Asn Ile Lys Arg Asp Gly Cys Ser Glu Asp 50 55 60 tgt acc ttt tgt ggt cag agc gcc ttt tat gat gca gat aaa gaa ccg 240 Cys Thr Phe Cys Gly Gln Ser Ala Phe Tyr Asp Ala Asp Lys Glu Pro 65 70 75 80 cat ccg ctg ccg gaa ccg gaa gaa gtt gtt cgt gca gcc ctg aaa gca 288 His Pro Leu Pro Glu Pro Glu Glu Val Val Arg Ala Ala Leu Lys Ala 85 90 95 aaa aaa gaa gaa gcc agc agc tat tgt ctg gtt gcc gca tgg cgt gaa 336 Lys Lys Glu Glu Ala Ser Ser Tyr Cys Leu Val Ala Ala Trp Arg Glu 100 105 110 ccg acc ccg gaa ggt ttt gaa aaa gtt tgt acc att att cag gaa att 384 Pro Thr Pro Glu Gly Phe Glu Lys Val Cys Thr Ile Ile Gln Glu Ile 115 120 125 aat acc cat gtt ggt att agc gtt gaa tgt agc ctg ggt ttt ctg acc 432 Asn Thr His Val Gly Ile Ser Val Glu Cys Ser Leu Gly Phe Leu Thr 130 135 140 cgt gaa cgt gca gca cgt ctg aaa ggt ctg ggt gtt aaa cgt tat aat 480 Arg Glu Arg Ala Ala Arg Leu Lys Gly Leu Gly Val Lys Arg Tyr Asn 145 150 155 160 cat aat ctg gaa acc gcc cgt agc aaa ttt ccg gaa att tgt agc acc 528 His Asn Leu Glu Thr Ala Arg Ser Lys Phe Pro Glu Ile Cys Ser Thr 165 170 175 cat acc tat gaa gat cgt ctg gat acc ctg gaa att gca cgt gaa gcc 576 His Thr Tyr Glu Asp Arg Leu Asp Thr Leu Glu Ile Ala Arg Glu Ala 180 185 190 ggt ctg gaa ctg tgt acc ggt ggt att att ggt atg ggt gaa agc cgt 624 Gly Leu Glu Leu Cys Thr Gly Gly Ile Ile Gly Met Gly Glu Ser Arg 195 200 205 ggt cag cgt att gaa ctg gca atg gaa ctg gca cgt att cgt ccg gaa 672 Gly Gln Arg Ile Glu Leu Ala Met Glu Leu Ala Arg Ile Arg Pro Glu 210 215 220 gaa gca acc gtt aat att ctg gtt ccg gtt cag ggt acc ccg atg gaa 720 Glu Ala Thr Val Asn Ile Leu Val Pro Val Gln Gly Thr Pro Met Glu 225 230 235 240 ctg cag gca ccg ctg ccg ccg ggt gaa gca gaa cgt ttt ttt gca ctg 768 Leu Gln Ala Pro Leu Pro Pro Gly Glu Ala Glu Arg Phe Phe Ala Leu 245 250 255 gtt cgt ttt ctg ctg ccg cgt agc gtt gtt aaa att agc ggt ggt cgt 816 Val Arg Phe Leu Leu Pro Arg Ser Val Val Lys Ile Ser Gly Gly Arg 260 265 270 gaa aaa gca ctg gat gat gat ggt cgt gca att ctg cgt ggt ggt gca 864 Glu Lys Ala Leu Asp Asp Asp Gly Arg Ala Ile Leu Arg Gly Gly Ala 275 280 285 aat ggt att att acc agc ggt tat ctg aca atg ggt ggt aat gat agc 912 Asn Gly Ile Ile Thr Ser Gly Tyr Leu Thr Met Gly Gly Asn Asp Ser 290 295 300 agc gca gat atg gaa atg att cgt gaa gca ggt ctg gaa gca taa 957 Ser Ala Asp Met Glu Met Ile Arg Glu Ala Gly Leu Glu Ala 305 310 315 <210> 55 <211> 318 <212> PRT <213> Cenarchaeum symbiosum A <400> 55 Met Gly Ile Ala Glu Cys Arg Asp Lys Val Leu Gly Gly Gly Glu Leu 1 5 10 15 Thr Lys Asp Glu Ala Arg Gly Leu Met Glu Ala Asp Val Thr Glu Leu 20 25 30 Ala Ala Ala Ala Asp Glu Ile Thr Arg Arg Phe Asn Gly Asp Gly Val 35 40 45 Asp Val Glu Gln Leu Asn Asn Ile Lys Arg Asp Gly Cys Ser Glu Asp 50 55 60 Cys Thr Phe Cys Gly Gln Ser Ala Phe Tyr Asp Ala Asp Lys Glu Pro 65 70 75 80 His Pro Leu Pro Glu Pro Glu Glu Val Val Arg Ala Ala Leu Lys Ala 85 90 95 Lys Lys Glu Glu Ala Ser Ser Tyr Cys Leu Val Ala Ala Trp Arg Glu 100 105 110 Pro Thr Pro Glu Gly Phe Glu Lys Val Cys Thr Ile Ile Gln Glu Ile 115 120 125 Asn Thr His Val Gly Ile Ser Val Glu Cys Ser Leu Gly Phe Leu Thr 130 135 140 Arg Glu Arg Ala Ala Arg Leu Lys Gly Leu Gly Val Lys Arg Tyr Asn 145 150 155 160 His Asn Leu Glu Thr Ala Arg Ser Lys Phe Pro Glu Ile Cys Ser Thr 165 170 175 His Thr Tyr Glu Asp Arg Leu Asp Thr Leu Glu Ile Ala Arg Glu Ala 180 185 190 Gly Leu Glu Leu Cys Thr Gly Gly Ile Ile Gly Met Gly Glu Ser Arg 195 200 205 Gly Gln Arg Ile Glu Leu Ala Met Glu Leu Ala Arg Ile Arg Pro Glu 210 215 220 Glu Ala Thr Val Asn Ile Leu Val Pro Val Gln Gly Thr Pro Met Glu 225 230 235 240 Leu Gln Ala Pro Leu Pro Pro Gly Glu Ala Glu Arg Phe Phe Ala Leu 245 250 255 Val Arg Phe Leu Leu Pro Arg Ser Val Val Lys Ile Ser Gly Gly Arg 260 265 270 Glu Lys Ala Leu Asp Asp Asp Gly Arg Ala Ile Leu Arg Gly Gly Ala 275 280 285 Asn Gly Ile Ile Thr Ser Gly Tyr Leu Thr Met Gly Gly Asn Asp Ser 290 295 300 Ser Ala Asp Met Glu Met Ile Arg Glu Ala Gly Leu Glu Ala 305 310 315 <210> 56 <211> 999 <212> DNA <213> Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 <220> <221> CDS <222> (1)..(999) <223> bioB gene encoding biotin synthase from Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 <400> 56 atg atg aaa att gat tat cag acc aat tgg att gat ctg gcc cgt cgt 48 Met Met Lys Ile Asp Tyr Gln Thr Asn Trp Ile Asp Leu Ala Arg Arg 1 5 10 15 gtt ctg gat ggt cgt ggt gtt acc cgt gaa gaa gca ctg gat att ctg 96 Val Leu Asp Gly Arg Gly Val Thr Arg Glu Glu Ala Leu Asp Ile Leu 20 25 30 cgt tct agc gat gat gaa ctg ctg gat ctg ctg gca gca gcc ttt ctg 144 Arg Ser Ser Asp Asp Glu Leu Leu Asp Leu Leu Ala Ala Ala Phe Leu 35 40 45 att cgt cgc cgc tat ttt ggc aaa aaa gtg aaa ctg aat atg att att 192 Ile Arg Arg Arg Tyr Phe Gly Lys Lys Val Lys Leu Asn Met Ile Ile 50 55 60 aat gcc aaa agc aaa atg tgc ccg gaa gat tgc gcc tat tgc agc cag 240 Asn Ala Lys Ser Lys Met Cys Pro Glu Asp Cys Ala Tyr Cys Ser Gln 65 70 75 80 agc gcc att agc aaa gca ccg gtt agc aaa tat ccg ctg gtt agt aaa 288 Ser Ala Ile Ser Lys Ala Pro Val Ser Lys Tyr Pro Leu Val Ser Lys 85 90 95 gaa gaa att att gcc ggt gca cgt gaa gca gaa cgt cgt aaa gca ggt 336 Glu Glu Ile Ile Ala Gly Ala Arg Glu Ala Glu Arg Arg Lys Ala Gly 100 105 110 acc tat tgt att gtt att agc ggt cgt cgt ccg agc gat cgt gaa att 384 Thr Tyr Cys Ile Val Ile Ser Gly Arg Arg Pro Ser Asp Arg Glu Ile 115 120 125 gaa cgt att gca gaa gca gtt gaa gaa att cgt gca acc acc acc ctg 432 Glu Arg Ile Ala Glu Ala Val Glu Glu Ile Arg Ala Thr Thr Thr Leu 130 135 140 aaa att tgt tgt tgt ctg ggt ctg ctg acc ccg gca cag gca gat cgt 480 Lys Ile Cys Cys Cys Leu Gly Leu Leu Thr Pro Ala Gln Ala Asp Arg 145 150 155 160 ctg gca cgt gcg ggt gtt cat cgt tat aat cat aat ctg aat acc agc 528 Leu Ala Arg Ala Gly Val His Arg Tyr Asn His Asn Leu Asn Thr Ser 165 170 175 cgt gat cgt tat ggt gat att tgt acc acc cat acc tat gat gat cgc 576 Arg Asp Arg Tyr Gly Asp Ile Cys Thr Thr His Thr Tyr Asp Asp Arg 180 185 190 gtt cgt acc ctg gaa cat gtg aaa gaa gca ggt att agc ccg tgt agc 624 Val Arg Thr Leu Glu His Val Lys Glu Ala Gly Ile Ser Pro Cys Ser 195 200 205 ggt gtt att ttt ggt atg ggt gaa agc gat gaa gaa gcc gtt gat atg 672 Gly Val Ile Phe Gly Met Gly Glu Ser Asp Glu Glu Ala Val Asp Met 210 215 220 gcc ttt gcc ctg aaa gaa atg gat gca gat agc att ccg tgt aat ttt 720 Ala Phe Ala Leu Lys Glu Met Asp Ala Asp Ser Ile Pro Cys Asn Phe 225 230 235 240 ctg aat ccg att ccg ggt acc ccg ctg gaa ggt atg gaa acc ctg aat 768 Leu Asn Pro Ile Pro Gly Thr Pro Leu Glu Gly Met Glu Thr Leu Asn 245 250 255 ccg cgt cgt tgt ctg aaa ctg ctg tgt atg atg cgt ttt gtt aat ccg 816 Pro Arg Arg Cys Leu Lys Leu Leu Cys Met Met Arg Phe Val Asn Pro 260 265 270 agc aaa gaa att cgt att gcg ggt ggt cgt gaa cgt aat ctg cgt agc 864 Ser Lys Glu Ile Arg Ile Ala Gly Gly Arg Glu Arg Asn Leu Arg Ser 275 280 285 ctg cag gtt ctg ggt ctg tat ccg gca aat agc att ttt gtt ggt gat 912 Leu Gln Val Leu Gly Leu Tyr Pro Ala Asn Ser Ile Phe Val Gly Asp 290 295 300 tat ctg acc acc ccg ggt cag gca ccg acc gaa gat tgg gca atg att 960 Tyr Leu Thr Thr Pro Gly Gln Ala Pro Thr Glu Asp Trp Ala Met Ile 305 310 315 320 gaa gat ctg ggt ttt gaa att gaa gaa tgt gca ctg taa 999 Glu Asp Leu Gly Phe Glu Ile Glu Glu Cys Ala Leu 325 330 <210> 57 <211> 332 <212> PRT <213> Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 <400> 57 Met Met Lys Ile Asp Tyr Gln Thr Asn Trp Ile Asp Leu Ala Arg Arg 1 5 10 15 Val Leu Asp Gly Arg Gly Val Thr Arg Glu Glu Ala Leu Asp Ile Leu 20 25 30 Arg Ser Ser Asp Asp Glu Leu Leu Asp Leu Leu Ala Ala Ala Phe Leu 35 40 45 Ile Arg Arg Arg Tyr Phe Gly Lys Lys Val Lys Leu Asn Met Ile Ile 50 55 60 Asn Ala Lys Ser Lys Met Cys Pro Glu Asp Cys Ala Tyr Cys Ser Gln 65 70 75 80 Ser Ala Ile Ser Lys Ala Pro Val Ser Lys Tyr Pro Leu Val Ser Lys 85 90 95 Glu Glu Ile Ile Ala Gly Ala Arg Glu Ala Glu Arg Arg Lys Ala Gly 100 105 110 Thr Tyr Cys Ile Val Ile Ser Gly Arg Arg Pro Ser Asp Arg Glu Ile 115 120 125 Glu Arg Ile Ala Glu Ala Val Glu Glu Ile Arg Ala Thr Thr Thr Leu 130 135 140 Lys Ile Cys Cys Cys Leu Gly Leu Leu Thr Pro Ala Gln Ala Asp Arg 145 150 155 160 Leu Ala Arg Ala Gly Val His Arg Tyr Asn His Asn Leu Asn Thr Ser 165 170 175 Arg Asp Arg Tyr Gly Asp Ile Cys Thr Thr His Thr Tyr Asp Asp Arg 180 185 190 Val Arg Thr Leu Glu His Val Lys Glu Ala Gly Ile Ser Pro Cys Ser 195 200 205 Gly Val Ile Phe Gly Met Gly Glu Ser Asp Glu Glu Ala Val Asp Met 210 215 220 Ala Phe Ala Leu Lys Glu Met Asp Ala Asp Ser Ile Pro Cys Asn Phe 225 230 235 240 Leu Asn Pro Ile Pro Gly Thr Pro Leu Glu Gly Met Glu Thr Leu Asn 245 250 255 Pro Arg Arg Cys Leu Lys Leu Leu Cys Met Met Arg Phe Val Asn Pro 260 265 270 Ser Lys Glu Ile Arg Ile Ala Gly Gly Arg Glu Arg Asn Leu Arg Ser 275 280 285 Leu Gln Val Leu Gly Leu Tyr Pro Ala Asn Ser Ile Phe Val Gly Asp 290 295 300 Tyr Leu Thr Thr Pro Gly Gln Ala Pro Thr Glu Asp Trp Ala Met Ile 305 310 315 320 Glu Asp Leu Gly Phe Glu Ile Glu Glu Cys Ala Leu 325 330 <210> 58 <211> 996 <212> DNA <213> Geobacillus thermoglucosidasius C56-YS93 <220> <221> CDS <222> (1)..(996) <223> bioB gene encoding biotin synthase from Geobacillus thermoglucosidasius C56-YS93 <400> 58 atg att aat tgg ctg gcc ctg gca gat cgt gtt att gca ggt cat gaa 48 Met Ile Asn Trp Leu Ala Leu Ala Asp Arg Val Ile Ala Gly His Glu 1 5 10 15 ctg acc gat gaa gaa gca ctg gca att ctg gat tgt ccg gat gaa gaa 96 Leu Thr Asp Glu Glu Ala Leu Ala Ile Leu Asp Cys Pro Asp Glu Glu 20 25 30 ctg ctg ctg ctg atg cag ggt gcc tat aat att cgt cgc acc tat tat 144 Leu Leu Leu Leu Met Gln Gly Ala Tyr Asn Ile Arg Arg Thr Tyr Tyr 35 40 45 ggc aat aaa gtt aaa ctg aat atg att att aat gcc aaa agc ggt ctg 192 Gly Asn Lys Val Lys Leu Asn Met Ile Ile Asn Ala Lys Ser Gly Leu 50 55 60 tgc ccg gaa aat tgc ggc tat tgc gca cag agc gca gtt agc acc gca 240 Cys Pro Glu Asn Cys Gly Tyr Cys Ala Gln Ser Ala Val Ser Thr Ala 65 70 75 80 ccg gtt aaa acc tat aaa atg gtt gat aaa gaa acc ctg att cgt ggt 288 Pro Val Lys Thr Tyr Lys Met Val Asp Lys Glu Thr Leu Ile Arg Gly 85 90 95 gca gaa gaa gca tat cgt atg cgt att ggt acc tat tgt att gtt gca 336 Ala Glu Glu Ala Tyr Arg Met Arg Ile Gly Thr Tyr Cys Ile Val Ala 100 105 110 agc ggt cgt ggt ccg agc gaa aaa gaa att gat acc gtt gtg agc gcc 384 Ser Gly Arg Gly Pro Ser Glu Lys Glu Ile Asp Thr Val Val Ser Ala 115 120 125 gtt aaa gaa att aaa gaa cgt ttt ggt ctg aaa att tgt gca tgt ctg 432 Val Lys Glu Ile Lys Glu Arg Phe Gly Leu Lys Ile Cys Ala Cys Leu 130 135 140 ggt att ctg aaa ccg gaa cag gca gca cgt ctg aaa gaa gcc ggt gtt 480 Gly Ile Leu Lys Pro Glu Gln Ala Ala Arg Leu Lys Glu Ala Gly Val 145 150 155 160 gat cgc tat aat cat aat att aat acc agc aaa gaa cat cat ccg aat 528 Asp Arg Tyr Asn His Asn Ile Asn Thr Ser Lys Glu His His Pro Asn 165 170 175 att acc acc agc cat acc tat gat gat cgc gtg cgt acc gtt gaa acc 576 Ile Thr Thr Ser His Thr Tyr Asp Asp Arg Val Arg Thr Val Glu Thr 180 185 190 gtt aaa cag gca ggt att agc ccg tgt agc ggt gtt att att ggt atg 624 Val Lys Gln Ala Gly Ile Ser Pro Cys Ser Gly Val Ile Ile Gly Met 195 200 205 cgt gaa acc aaa cag gat gtt att aat atg gca cgt agt ctg cgc att 672 Arg Glu Thr Lys Gln Asp Val Ile Asn Met Ala Arg Ser Leu Arg Ile 210 215 220 ctg gat gca gat agc att ccg gtt aat ttt ctg cat gca att gat ggt 720 Leu Asp Ala Asp Ser Ile Pro Val Asn Phe Leu His Ala Ile Asp Gly 225 230 235 240 acc ccg ctg gca ggt acc aat gaa ctg gat ccg cgt tat tgt ctg aaa 768 Thr Pro Leu Ala Gly Thr Asn Glu Leu Asp Pro Arg Tyr Cys Leu Lys 245 250 255 gtt ctg gca ctg ttt cgt tat atg aat ccg acc aaa gaa att cgt att 816 Val Leu Ala Leu Phe Arg Tyr Met Asn Pro Thr Lys Glu Ile Arg Ile 260 265 270 gcc ggt ggt cgt gaa gtt aat ctg cgt agc ctg cag ccg ctg ggt ctg 864 Ala Gly Gly Arg Glu Val Asn Leu Arg Ser Leu Gln Pro Leu Gly Leu 275 280 285 tat gca gca aat agc att ttt gtt ggt gat tat ctg acc acc gca ggt 912 Tyr Ala Ala Asn Ser Ile Phe Val Gly Asp Tyr Leu Thr Thr Ala Gly 290 295 300 cag gaa aaa agc gaa gat tat cgc atg ctg gaa gat ctg ggt ttt gaa 960 Gln Glu Lys Ser Glu Asp Tyr Arg Met Leu Glu Asp Leu Gly Phe Glu 305 310 315 320 att gat ttt gcc gaa gaa cag cag gtt gtt tgt taa 996 Ile Asp Phe Ala Glu Glu Gln Gln Val Val Cys 325 330 <210> 59 <211> 331 <212> PRT <213> Geobacillus thermoglucosidasius C56-YS93 <400> 59 Met Ile Asn Trp Leu Ala Leu Ala Asp Arg Val Ile Ala Gly His Glu 1 5 10 15 Leu Thr Asp Glu Glu Ala Leu Ala Ile Leu Asp Cys Pro Asp Glu Glu 20 25 30 Leu Leu Leu Leu Met Gln Gly Ala Tyr Asn Ile Arg Arg Thr Tyr Tyr 35 40 45 Gly Asn Lys Val Lys Leu Asn Met Ile Ile Asn Ala Lys Ser Gly Leu 50 55 60 Cys Pro Glu Asn Cys Gly Tyr Cys Ala Gln Ser Ala Val Ser Thr Ala 65 70 75 80 Pro Val Lys Thr Tyr Lys Met Val Asp Lys Glu Thr Leu Ile Arg Gly 85 90 95 Ala Glu Glu Ala Tyr Arg Met Arg Ile Gly Thr Tyr Cys Ile Val Ala 100 105 110 Ser Gly Arg Gly Pro Ser Glu Lys Glu Ile Asp Thr Val Val Ser Ala 115 120 125 Val Lys Glu Ile Lys Glu Arg Phe Gly Leu Lys Ile Cys Ala Cys Leu 130 135 140 Gly Ile Leu Lys Pro Glu Gln Ala Ala Arg Leu Lys Glu Ala Gly Val 145 150 155 160 Asp Arg Tyr Asn His Asn Ile Asn Thr Ser Lys Glu His His Pro Asn 165 170 175 Ile Thr Thr Ser His Thr Tyr Asp Asp Arg Val Arg Thr Val Glu Thr 180 185 190 Val Lys Gln Ala Gly Ile Ser Pro Cys Ser Gly Val Ile Ile Gly Met 195 200 205 Arg Glu Thr Lys Gln Asp Val Ile Asn Met Ala Arg Ser Leu Arg Ile 210 215 220 Leu Asp Ala Asp Ser Ile Pro Val Asn Phe Leu His Ala Ile Asp Gly 225 230 235 240 Thr Pro Leu Ala Gly Thr Asn Glu Leu Asp Pro Arg Tyr Cys Leu Lys 245 250 255 Val Leu Ala Leu Phe Arg Tyr Met Asn Pro Thr Lys Glu Ile Arg Ile 260 265 270 Ala Gly Gly Arg Glu Val Asn Leu Arg Ser Leu Gln Pro Leu Gly Leu 275 280 285 Tyr Ala Ala Asn Ser Ile Phe Val Gly Asp Tyr Leu Thr Thr Ala Gly 290 295 300 Gln Glu Lys Ser Glu Asp Tyr Arg Met Leu Glu Asp Leu Gly Phe Glu 305 310 315 320 Ile Asp Phe Ala Glu Glu Gln Gln Val Val Cys 325 330 <210> 60 <211> 1008 <212> DNA <213> Bacillus subtilis subsp. subtilis str. 168 <220> <221> CDS <222> (1)..(1008) <223> bioB gene encoding biotin synthase from Bacillus subtilis subsp. subtilis str. 168 <400> 60 atg aat cag tgg atg gaa ctg gca gat cgt gtt ctg gcc ggt gca gaa 48 Met Asn Gln Trp Met Glu Leu Ala Asp Arg Val Leu Ala Gly Ala Glu 1 5 10 15 gtt acc gat gaa gaa gca ctg agc att ctg cat tgc ccg gat gaa gat 96 Val Thr Asp Glu Glu Ala Leu Ser Ile Leu His Cys Pro Asp Glu Asp 20 25 30 atc ctg ctg ctg atg cat ggt gcc ttt cat att cgc aaa cat ttt tat 144 Ile Leu Leu Leu Met His Gly Ala Phe His Ile Arg Lys His Phe Tyr 35 40 45 ggc aaa aaa gtg aaa ctg aat atg att atg aac gcc aaa agc ggt ctg 192 Gly Lys Lys Val Lys Leu Asn Met Ile Met Asn Ala Lys Ser Gly Leu 50 55 60 tgt ccg gaa aat tgc ggt tat tgc agc cag agc gca att agc aaa gca 240 Cys Pro Glu Asn Cys Gly Tyr Cys Ser Gln Ser Ala Ile Ser Lys Ala 65 70 75 80 ccg att gaa agc tat cgt atg gtt aat aaa gaa acc ctg ctg gaa ggt 288 Pro Ile Glu Ser Tyr Arg Met Val Asn Lys Glu Thr Leu Leu Glu Gly 85 90 95 gca aaa cgt gca cat gat ctg aat att ggt acc tat tgt att gtt gca 336 Ala Lys Arg Ala His Asp Leu Asn Ile Gly Thr Tyr Cys Ile Val Ala 100 105 110 agc ggt cgt ggt ccg agc aat cgc gaa gtt gat cag gtt gtt gat gca 384 Ser Gly Arg Gly Pro Ser Asn Arg Glu Val Asp Gln Val Val Asp Ala 115 120 125 gtt cag gaa att aaa gaa acc tat ggt ctg aaa att tgt gca tgt ctg 432 Val Gln Glu Ile Lys Glu Thr Tyr Gly Leu Lys Ile Cys Ala Cys Leu 130 135 140 ggt ctg ctg aaa ccg gaa cag gca aaa cgt ctg aaa gat gca ggc gtt 480 Gly Leu Leu Lys Pro Glu Gln Ala Lys Arg Leu Lys Asp Ala Gly Val 145 150 155 160 gat cgt tat aat cat aat ctg aat acc agc cag cgt aac cat agc aat 528 Asp Arg Tyr Asn His Asn Leu Asn Thr Ser Gln Arg Asn His Ser Asn 165 170 175 att acc acc agc cat acc tat gat gat cgt gtg aat acc gtt gaa att 576 Ile Thr Thr Ser His Thr Tyr Asp Asp Arg Val Asn Thr Val Glu Ile 180 185 190 gcc aaa gaa agt ggt ctg agc ccg tgt agc ggt gca att att ggt atg 624 Ala Lys Glu Ser Gly Leu Ser Pro Cys Ser Gly Ala Ile Ile Gly Met 195 200 205 aaa gaa acc aaa cag gat gtt att gat att gcg aaa agc ctg aaa gca 672 Lys Glu Thr Lys Gln Asp Val Ile Asp Ile Ala Lys Ser Leu Lys Ala 210 215 220 ctg gat gca gat agc att ccg gtg aat ttt ctg cat gca att gat ggt 720 Leu Asp Ala Asp Ser Ile Pro Val Asn Phe Leu His Ala Ile Asp Gly 225 230 235 240 acc ccg ctg gaa ggt gtt aat gaa ctg aat ccg ctg tat tgt ctg aaa 768 Thr Pro Leu Glu Gly Val Asn Glu Leu Asn Pro Leu Tyr Cys Leu Lys 245 250 255 gtt ctg gca ctg ttt cgt ttt att aat ccg agc aaa gaa att cgt att 816 Val Leu Ala Leu Phe Arg Phe Ile Asn Pro Ser Lys Glu Ile Arg Ile 260 265 270 agc ggt ggt cgt gaa gtt aat ctg cgt acc ctg cag ccg ctg ggt ctg 864 Ser Gly Gly Arg Glu Val Asn Leu Arg Thr Leu Gln Pro Leu Gly Leu 275 280 285 tat gca gca aat agc att ttt gtt ggt gat tat ctg acc acc gca ggt 912 Tyr Ala Ala Asn Ser Ile Phe Val Gly Asp Tyr Leu Thr Thr Ala Gly 290 295 300 cag gaa gaa acc gaa gat cat aaa atg ctg agc gat ctg ggt ttt gaa 960 Gln Glu Glu Thr Glu Asp His Lys Met Leu Ser Asp Leu Gly Phe Glu 305 310 315 320 gtt gaa agc gtt gaa gaa atg aaa gca agc ctg agc gca aaa agc taa 1008 Val Glu Ser Val Glu Glu Met Lys Ala Ser Leu Ser Ala Lys Ser 325 330 335 <210> 61 <211> 335 <212> PRT <213> Bacillus subtilis subsp. subtilis str. 168 <400> 61 Met Asn Gln Trp Met Glu Leu Ala Asp Arg Val Leu Ala Gly Ala Glu 1 5 10 15 Val Thr Asp Glu Glu Ala Leu Ser Ile Leu His Cys Pro Asp Glu Asp 20 25 30 Ile Leu Leu Leu Met His Gly Ala Phe His Ile Arg Lys His Phe Tyr 35 40 45 Gly Lys Lys Val Lys Leu Asn Met Ile Met Asn Ala Lys Ser Gly Leu 50 55 60 Cys Pro Glu Asn Cys Gly Tyr Cys Ser Gln Ser Ala Ile Ser Lys Ala 65 70 75 80 Pro Ile Glu Ser Tyr Arg Met Val Asn Lys Glu Thr Leu Leu Glu Gly 85 90 95 Ala Lys Arg Ala His Asp Leu Asn Ile Gly Thr Tyr Cys Ile Val Ala 100 105 110 Ser Gly Arg Gly Pro Ser Asn Arg Glu Val Asp Gln Val Val Asp Ala 115 120 125 Val Gln Glu Ile Lys Glu Thr Tyr Gly Leu Lys Ile Cys Ala Cys Leu 130 135 140 Gly Leu Leu Lys Pro Glu Gln Ala Lys Arg Leu Lys Asp Ala Gly Val 145 150 155 160 Asp Arg Tyr Asn His Asn Leu Asn Thr Ser Gln Arg Asn His Ser Asn 165 170 175 Ile Thr Thr Ser His Thr Tyr Asp Asp Arg Val Asn Thr Val Glu Ile 180 185 190 Ala Lys Glu Ser Gly Leu Ser Pro Cys Ser Gly Ala Ile Ile Gly Met 195 200 205 Lys Glu Thr Lys Gln Asp Val Ile Asp Ile Ala Lys Ser Leu Lys Ala 210 215 220 Leu Asp Ala Asp Ser Ile Pro Val Asn Phe Leu His Ala Ile Asp Gly 225 230 235 240 Thr Pro Leu Glu Gly Val Asn Glu Leu Asn Pro Leu Tyr Cys Leu Lys 245 250 255 Val Leu Ala Leu Phe Arg Phe Ile Asn Pro Ser Lys Glu Ile Arg Ile 260 265 270 Ser Gly Gly Arg Glu Val Asn Leu Arg Thr Leu Gln Pro Leu Gly Leu 275 280 285 Tyr Ala Ala Asn Ser Ile Phe Val Gly Asp Tyr Leu Thr Thr Ala Gly 290 295 300 Gln Glu Glu Thr Glu Asp His Lys Met Leu Ser Asp Leu Gly Phe Glu 305 310 315 320 Val Glu Ser Val Glu Glu Met Lys Ala Ser Leu Ser Ala Lys Ser 325 330 335 <210> 62 <211> 996 <212> DNA <213> Lysinibacillus sphaericus <220> <221> CDS <222> (1)..(996) <223> bioB gene encoding biotin synthase from Lysinibacillus sphaericus <400> 62 atg aat ttt ctg cag gtt gcc cag gaa gtt att gat ggc aaa att att 48 Met Asn Phe Leu Gln Val Ala Gln Glu Val Ile Asp Gly Lys Ile Ile 1 5 10 15 agc aat gaa gaa gcc ctg gcc att ctg aat agc aaa gat gat gaa ctg 96 Ser Asn Glu Glu Ala Leu Ala Ile Leu Asn Ser Lys Asp Asp Glu Leu 20 25 30 ctg cag ctg atg gat ggt gca ttt gcc att cgc cgc cat tat tat ggt 144 Leu Gln Leu Met Asp Gly Ala Phe Ala Ile Arg Arg His Tyr Tyr Gly 35 40 45 aaa aaa gtg aaa ctg aac atg att atg aac gcc aaa agc ggt tat tgc 192 Lys Lys Val Lys Leu Asn Met Ile Met Asn Ala Lys Ser Gly Tyr Cys 50 55 60 ccg gaa gat tgt ggt tat tgt agc cag agc agc aaa agc acc gca ccg 240 Pro Glu Asp Cys Gly Tyr Cys Ser Gln Ser Ser Lys Ser Thr Ala Pro 65 70 75 80 att gaa aaa tat ccg ttt att acc aaa gaa gaa att ctg gcc ggt gcc 288 Ile Glu Lys Tyr Pro Phe Ile Thr Lys Glu Glu Ile Leu Ala Gly Ala 85 90 95 aaa cgt gcc ttt gat aat aaa att ggt acc tat tgc att gtt gcc agc 336 Lys Arg Ala Phe Asp Asn Lys Ile Gly Thr Tyr Cys Ile Val Ala Ser 100 105 110 ggt cgt ggt ccg acc cgt aaa gat gtt aat gtt gtg agc gaa gca gtt 384 Gly Arg Gly Pro Thr Arg Lys Asp Val Asn Val Val Ser Glu Ala Val 115 120 125 acc gaa att aaa gaa aaa tat ggc ctg aaa gtt tgt gca tgt ctg ggt 432 Thr Glu Ile Lys Glu Lys Tyr Gly Leu Lys Val Cys Ala Cys Leu Gly 130 135 140 ctg ctg aaa gaa gaa cag gca cag cag ctg aaa gaa gcc ggt gtt gat 480 Leu Leu Lys Glu Glu Gln Ala Gln Gln Leu Lys Glu Ala Gly Val Asp 145 150 155 160 cgc tat aat cat aat ctg aac acc agc gaa cgt cat cat agc ttt att 528 Arg Tyr Asn His Asn Leu Asn Thr Ser Glu Arg His His Ser Phe Ile 165 170 175 acc acc agc cat acc tat gaa gat cgt gtg aac acc gtg gaa att gtg 576 Thr Thr Ser His Thr Tyr Glu Asp Arg Val Asn Thr Val Glu Ile Val 180 185 190 aaa aaa cat ggt att agc ccg tgt agc ggt gca att att ggt atg aaa 624 Lys Lys His Gly Ile Ser Pro Cys Ser Gly Ala Ile Ile Gly Met Lys 195 200 205 gaa acc cgt gaa gat gtt gtt aat att gca cgt gca ctg cat cag ctg 672 Glu Thr Arg Glu Asp Val Val Asn Ile Ala Arg Ala Leu His Gln Leu 210 215 220 gat gca gat agc att ccg gtt aat ttt ctg aat gca att gat ggt acc 720 Asp Ala Asp Ser Ile Pro Val Asn Phe Leu Asn Ala Ile Asp Gly Thr 225 230 235 240 aaa ctg gaa ggt acc cgt gat ctg aat ccg cgt tat tgt ctg aaa gtg 768 Lys Leu Glu Gly Thr Arg Asp Leu Asn Pro Arg Tyr Cys Leu Lys Val 245 250 255 ctg gca ctg ttt cgt tat att aat ccg acc aaa gaa att cgt att agc 816 Leu Ala Leu Phe Arg Tyr Ile Asn Pro Thr Lys Glu Ile Arg Ile Ser 260 265 270 ggt ggt cgt gaa att aat ctg ggt agc ctg cag ccg ctg ggt ctg tat 864 Gly Gly Arg Glu Ile Asn Leu Gly Ser Leu Gln Pro Leu Gly Leu Tyr 275 280 285 gca gca aat agc att ttt gtt ggt gat tat ctg acc acc gca ggt cag 912 Ala Ala Asn Ser Ile Phe Val Gly Asp Tyr Leu Thr Thr Ala Gly Gln 290 295 300 gaa gcc aat agc gat tat cgt atg ctg gaa gat ctg ggt ttt gaa att 960 Glu Ala Asn Ser Asp Tyr Arg Met Leu Glu Asp Leu Gly Phe Glu Ile 305 310 315 320 gaa ctg acc cag aaa cag gaa gca gca ttt tgt taa 996 Glu Leu Thr Gln Lys Gln Glu Ala Ala Phe Cys 325 330 <210> 63 <211> 331 <212> PRT <213> Lysinibacillus sphaericus <400> 63 Met Asn Phe Leu Gln Val Ala Gln Glu Val Ile Asp Gly Lys Ile Ile 1 5 10 15 Ser Asn Glu Glu Ala Leu Ala Ile Leu Asn Ser Lys Asp Asp Glu Leu 20 25 30 Leu Gln Leu Met Asp Gly Ala Phe Ala Ile Arg Arg His Tyr Tyr Gly 35 40 45 Lys Lys Val Lys Leu Asn Met Ile Met Asn Ala Lys Ser Gly Tyr Cys 50 55 60 Pro Glu Asp Cys Gly Tyr Cys Ser Gln Ser Ser Lys Ser Thr Ala Pro 65 70 75 80 Ile Glu Lys Tyr Pro Phe Ile Thr Lys Glu Glu Ile Leu Ala Gly Ala 85 90 95 Lys Arg Ala Phe Asp Asn Lys Ile Gly Thr Tyr Cys Ile Val Ala Ser 100 105 110 Gly Arg Gly Pro Thr Arg Lys Asp Val Asn Val Val Ser Glu Ala Val 115 120 125 Thr Glu Ile Lys Glu Lys Tyr Gly Leu Lys Val Cys Ala Cys Leu Gly 130 135 140 Leu Leu Lys Glu Glu Gln Ala Gln Gln Leu Lys Glu Ala Gly Val Asp 145 150 155 160 Arg Tyr Asn His Asn Leu Asn Thr Ser Glu Arg His His Ser Phe Ile 165 170 175 Thr Thr Ser His Thr Tyr Glu Asp Arg Val Asn Thr Val Glu Ile Val 180 185 190 Lys Lys His Gly Ile Ser Pro Cys Ser Gly Ala Ile Ile Gly Met Lys 195 200 205 Glu Thr Arg Glu Asp Val Val Asn Ile Ala Arg Ala Leu His Gln Leu 210 215 220 Asp Ala Asp Ser Ile Pro Val Asn Phe Leu Asn Ala Ile Asp Gly Thr 225 230 235 240 Lys Leu Glu Gly Thr Arg Asp Leu Asn Pro Arg Tyr Cys Leu Lys Val 245 250 255 Leu Ala Leu Phe Arg Tyr Ile Asn Pro Thr Lys Glu Ile Arg Ile Ser 260 265 270 Gly Gly Arg Glu Ile Asn Leu Gly Ser Leu Gln Pro Leu Gly Leu Tyr 275 280 285 Ala Ala Asn Ser Ile Phe Val Gly Asp Tyr Leu Thr Thr Ala Gly Gln 290 295 300 Glu Ala Asn Ser Asp Tyr Arg Met Leu Glu Asp Leu Gly Phe Glu Ile 305 310 315 320 Glu Leu Thr Gln Lys Gln Glu Ala Ala Phe Cys 325 330 <210> 64 <211> 1002 <212> DNA <213> Methylococcus capsulatus str. Bath <220> <221> CDS <222> (1)..(1002) <223> bioB gene encoding biotin synthase from Methylococcus capsulatus str. Bath <400> 64 atg cat gca gaa gtt gca gtt atg acc aat cag gaa cgt gca gaa gaa 48 Met His Ala Glu Val Ala Val Met Thr Asn Gln Glu Arg Ala Glu Glu 1 5 10 15 ccg gtt ctg cgt cat gat tgg acc cag ggt gaa gca gaa gca ctg ttt 96 Pro Val Leu Arg His Asp Trp Thr Gln Gly Glu Ala Glu Ala Leu Phe 20 25 30 gca ctg ccg ttt aat gaa ctg ctg ttt cag gca cag acc att cat cgt 144 Ala Leu Pro Phe Asn Glu Leu Leu Phe Gln Ala Gln Thr Ile His Arg 35 40 45 cgt cat ttt gat ccg aat gaa gtt cag gtt agc agc ctg ctg agc att 192 Arg His Phe Asp Pro Asn Glu Val Gln Val Ser Ser Leu Leu Ser Ile 50 55 60 aaa acc ggt gca tgt agc gaa gat tgt gca tat tgt ccg cag agc gca 240 Lys Thr Gly Ala Cys Ser Glu Asp Cys Ala Tyr Cys Pro Gln Ser Ala 65 70 75 80 cat tat gaa acc ggt gtt aaa cgt gaa agc ctg atg agc ctg gaa gat 288 His Tyr Glu Thr Gly Val Lys Arg Glu Ser Leu Met Ser Leu Glu Asp 85 90 95 gtt ctg gaa gca gca cag cgt gca cgt gaa gaa ggt gca acc cgt ttt 336 Val Leu Glu Ala Ala Gln Arg Ala Arg Glu Glu Gly Ala Thr Arg Phe 100 105 110 tgt atg ggt gca gca tgg cgt agc ccg cgt gat ggt gat ctg gaa gca 384 Cys Met Gly Ala Ala Trp Arg Ser Pro Arg Asp Gly Asp Leu Glu Ala 115 120 125 att gca gca atg gtt gaa ggt gtt aaa gca ctg ggt atg gaa acc tgt 432 Ile Ala Ala Met Val Glu Gly Val Lys Ala Leu Gly Met Glu Thr Cys 130 135 140 gtt acc gcc ggt atg ctg agt gat gaa cag gcc cgt cgt ctg aaa gaa 480 Val Thr Ala Gly Met Leu Ser Asp Glu Gln Ala Arg Arg Leu Lys Glu 145 150 155 160 gcc ggt ctg gat tat tat aat cat aat ctg gat acc agc gaa agc tat 528 Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu Asp Thr Ser Glu Ser Tyr 165 170 175 tat ggc gaa att att acc acc cgc acc tat cag gat cgt ctg gat acc 576 Tyr Gly Glu Ile Ile Thr Thr Arg Thr Tyr Gln Asp Arg Leu Asp Thr 180 185 190 ctg cag cgt gtt cgt gat gca ggt atg cat gtt tgt tgt ggt ggt att 624 Leu Gln Arg Val Arg Asp Ala Gly Met His Val Cys Cys Gly Gly Ile 195 200 205 gtt ggt atg ggt gaa agc gca gca gat cgt gca ggt ctg ctg att ggt 672 Val Gly Met Gly Glu Ser Ala Ala Asp Arg Ala Gly Leu Leu Ile Gly 210 215 220 ctg gca aat ctg ccg cgt cat ccg gaa agc gtt ccg att aat ctg ctg 720 Leu Ala Asn Leu Pro Arg His Pro Glu Ser Val Pro Ile Asn Leu Leu 225 230 235 240 gtt cgt gtt gaa ggt acc ccg ctg gca gat acc gca gca ctg gat ccg 768 Val Arg Val Glu Gly Thr Pro Leu Ala Asp Thr Ala Ala Leu Asp Pro 245 250 255 ttt gat ttt gtt cgt acc gtt gca gtt gca cgt att atg atg ccg gca 816 Phe Asp Phe Val Arg Thr Val Ala Val Ala Arg Ile Met Met Pro Ala 260 265 270 agc cgt gtt cgt ctg agc gca ggt cgt agc gat atg agc gat gaa atg 864 Ser Arg Val Arg Leu Ser Ala Gly Arg Ser Asp Met Ser Asp Glu Met 275 280 285 cag gca ctg tgt ttt ctg gcc ggt gca aat agc att ttt tat ggt gat 912 Gln Ala Leu Cys Phe Leu Ala Gly Ala Asn Ser Ile Phe Tyr Gly Asp 290 295 300 cgt ctg ctg acc acc gaa aat ccg cag gcc cag cgt gat cgc cgt ctg 960 Arg Leu Leu Thr Thr Glu Asn Pro Gln Ala Gln Arg Asp Arg Arg Leu 305 310 315 320 ttt gcc cgt ctg ggt ctg cgt atg gca ggt ctg ggt tgt taa 1002 Phe Ala Arg Leu Gly Leu Arg Met Ala Gly Leu Gly Cys 325 330 <210> 65 <211> 333 <212> PRT <213> Methylococcus capsulatus str. Bath <400> 65 Met His Ala Glu Val Ala Val Met Thr Asn Gln Glu Arg Ala Glu Glu 1 5 10 15 Pro Val Leu Arg His Asp Trp Thr Gln Gly Glu Ala Glu Ala Leu Phe 20 25 30 Ala Leu Pro Phe Asn Glu Leu Leu Phe Gln Ala Gln Thr Ile His Arg 35 40 45 Arg His Phe Asp Pro Asn Glu Val Gln Val Ser Ser Leu Leu Ser Ile 50 55 60 Lys Thr Gly Ala Cys Ser Glu Asp Cys Ala Tyr Cys Pro Gln Ser Ala 65 70 75 80 His Tyr Glu Thr Gly Val Lys Arg Glu Ser Leu Met Ser Leu Glu Asp 85 90 95 Val Leu Glu Ala Ala Gln Arg Ala Arg Glu Glu Gly Ala Thr Arg Phe 100 105 110 Cys Met Gly Ala Ala Trp Arg Ser Pro Arg Asp Gly Asp Leu Glu Ala 115 120 125 Ile Ala Ala Met Val Glu Gly Val Lys Ala Leu Gly Met Glu Thr Cys 130 135 140 Val Thr Ala Gly Met Leu Ser Asp Glu Gln Ala Arg Arg Leu Lys Glu 145 150 155 160 Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu Asp Thr Ser Glu Ser Tyr 165 170 175 Tyr Gly Glu Ile Ile Thr Thr Arg Thr Tyr Gln Asp Arg Leu Asp Thr 180 185 190 Leu Gln Arg Val Arg Asp Ala Gly Met His Val Cys Cys Gly Gly Ile 195 200 205 Val Gly Met Gly Glu Ser Ala Ala Asp Arg Ala Gly Leu Leu Ile Gly 210 215 220 Leu Ala Asn Leu Pro Arg His Pro Glu Ser Val Pro Ile Asn Leu Leu 225 230 235 240 Val Arg Val Glu Gly Thr Pro Leu Ala Asp Thr Ala Ala Leu Asp Pro 245 250 255 Phe Asp Phe Val Arg Thr Val Ala Val Ala Arg Ile Met Met Pro Ala 260 265 270 Ser Arg Val Arg Leu Ser Ala Gly Arg Ser Asp Met Ser Asp Glu Met 275 280 285 Gln Ala Leu Cys Phe Leu Ala Gly Ala Asn Ser Ile Phe Tyr Gly Asp 290 295 300 Arg Leu Leu Thr Thr Glu Asn Pro Gln Ala Gln Arg Asp Arg Arg Leu 305 310 315 320 Phe Ala Arg Leu Gly Leu Arg Met Ala Gly Leu Gly Cys 325 330 <210> 66 <211> 1041 <212> DNA <213> Leclercia adecarboxylata <220> <221> CDS <222> (1)..(1041) <223> bioB gene encoding biotin synthase from Leclercia adecarboxylata <400> 66 atg gca cat cag acc cgt tgg acc ctg agc cag gtt acc gca ctg ttt 48 Met Ala His Gln Thr Arg Trp Thr Leu Ser Gln Val Thr Ala Leu Phe 1 5 10 15 gaa aaa ccg ctg ctg gaa ctg ctg ttt gaa gca cag cag att cat cgt 96 Glu Lys Pro Leu Leu Glu Leu Leu Phe Glu Ala Gln Gln Ile His Arg 20 25 30 cag cat ttt gat ccg cag cag att cag gtt agc acc ctg ctg agc att 144 Gln His Phe Asp Pro Gln Gln Ile Gln Val Ser Thr Leu Leu Ser Ile 35 40 45 aaa acc ggt gca tgt ccg gaa gat tgt aaa tat tgt ccg cag agc gca 192 Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys Tyr Cys Pro Gln Ser Ala 50 55 60 cgt tat aaa acc ggt ctg gaa tca gaa cgt ctg atg gaa gtt gaa cag 240 Arg Tyr Lys Thr Gly Leu Glu Ser Glu Arg Leu Met Glu Val Glu Gln 65 70 75 80 gtt ctg gaa agc gca cgt cag gca aaa aat gca ggt agc acc cgt ttt 288 Val Leu Glu Ser Ala Arg Gln Ala Lys Asn Ala Gly Ser Thr Arg Phe 85 90 95 tgt atg ggt gca gca tgg aaa aat ccg cat gaa cgt gat atg ccg tat 336 Cys Met Gly Ala Ala Trp Lys Asn Pro His Glu Arg Asp Met Pro Tyr 100 105 110 ctg gaa cag atg gtt cag ggt gtt aaa gca atg ggt ctg gaa gca tgt 384 Leu Glu Gln Met Val Gln Gly Val Lys Ala Met Gly Leu Glu Ala Cys 115 120 125 atg acc ctg ggt acc ctg gat gat acc cag gca cag cgt ctg gca agc 432 Met Thr Leu Gly Thr Leu Asp Asp Thr Gln Ala Gln Arg Leu Ala Ser 130 135 140 gca ggt ctg gat tat tat aat cat aat ctg gat acc agc ccg gaa ttt 480 Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu Asp Thr Ser Pro Glu Phe 145 150 155 160 tat ggc aat att att acc acc cgc acc tat cag gaa cgc ctg gat acc 528 Tyr Gly Asn Ile Ile Thr Thr Arg Thr Tyr Gln Glu Arg Leu Asp Thr 165 170 175 ctg gat aaa gtt cgt gat gca ggt att aaa gtt tgt agc ggt ggt att 576 Leu Asp Lys Val Arg Asp Ala Gly Ile Lys Val Cys Ser Gly Gly Ile 180 185 190 gtt ggt ctg ggt gaa acc gtt acc gat cgt gca ggt ctg ctg ctg cag 624 Val Gly Leu Gly Glu Thr Val Thr Asp Arg Ala Gly Leu Leu Leu Gln 195 200 205 ctg gca aat ctg ccg acc ccg ccg gaa agc gtt ccg att aat atg ctg 672 Leu Ala Asn Leu Pro Thr Pro Pro Glu Ser Val Pro Ile Asn Met Leu 210 215 220 gtt aaa gtt aaa ggt acc ccg ctg gcc gat aat gat gat gtt gat gca 720 Val Lys Val Lys Gly Thr Pro Leu Ala Asp Asn Asp Asp Val Asp Ala 225 230 235 240 ttt gat ttt att cgt acc att gca gtg gcc cgt gtg atg atg ccg acc 768 Phe Asp Phe Ile Arg Thr Ile Ala Val Ala Arg Val Met Met Pro Thr 245 250 255 agc ttt gtt cgt ctg agc gcc ggt cgt gaa cag atg aat gaa cag acc 816 Ser Phe Val Arg Leu Ser Ala Gly Arg Glu Gln Met Asn Glu Gln Thr 260 265 270 cag gcc atg tgt ttt atg gcc ggt gca aat agc att ttt tat ggt tgt 864 Gln Ala Met Cys Phe Met Ala Gly Ala Asn Ser Ile Phe Tyr Gly Cys 275 280 285 aaa ctg ctg acc acc ccg aat ccg gaa gaa gat aaa gat gtt cag ctg 912 Lys Leu Leu Thr Thr Pro Asn Pro Glu Glu Asp Lys Asp Val Gln Leu 290 295 300 ttt cgt aaa ctg ggt ctg aat ccg cag cag acc gca gtt ctg acc ggt 960 Phe Arg Lys Leu Gly Leu Asn Pro Gln Gln Thr Ala Val Leu Thr Gly 305 310 315 320 gat aat gaa cag cag cat cag ctg gaa cag cag ctg att aat gca gat 1008 Asp Asn Glu Gln Gln His Gln Leu Glu Gln Gln Leu Ile Asn Ala Asp 325 330 335 acc gat cag ttt tat aat gca gca acc gtt taa 1041 Thr Asp Gln Phe Tyr Asn Ala Ala Thr Val 340 345 <210> 67 <211> 346 <212> PRT <213> Leclercia adecarboxylata <400> 67 Met Ala His Gln Thr Arg Trp Thr Leu Ser Gln Val Thr Ala Leu Phe 1 5 10 15 Glu Lys Pro Leu Leu Glu Leu Leu Phe Glu Ala Gln Gln Ile His Arg 20 25 30 Gln His Phe Asp Pro Gln Gln Ile Gln Val Ser Thr Leu Leu Ser Ile 35 40 45 Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys Tyr Cys Pro Gln Ser Ala 50 55 60 Arg Tyr Lys Thr Gly Leu Glu Ser Glu Arg Leu Met Glu Val Glu Gln 65 70 75 80 Val Leu Glu Ser Ala Arg Gln Ala Lys Asn Ala Gly Ser Thr Arg Phe 85 90 95 Cys Met Gly Ala Ala Trp Lys Asn Pro His Glu Arg Asp Met Pro Tyr 100 105 110 Leu Glu Gln Met Val Gln Gly Val Lys Ala Met Gly Leu Glu Ala Cys 115 120 125 Met Thr Leu Gly Thr Leu Asp Asp Thr Gln Ala Gln Arg Leu Ala Ser 130 135 140 Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu Asp Thr Ser Pro Glu Phe 145 150 155 160 Tyr Gly Asn Ile Ile Thr Thr Arg Thr Tyr Gln Glu Arg Leu Asp Thr 165 170 175 Leu Asp Lys Val Arg Asp Ala Gly Ile Lys Val Cys Ser Gly Gly Ile 180 185 190 Val Gly Leu Gly Glu Thr Val Thr Asp Arg Ala Gly Leu Leu Leu Gln 195 200 205 Leu Ala Asn Leu Pro Thr Pro Pro Glu Ser Val Pro Ile Asn Met Leu 210 215 220 Val Lys Val Lys Gly Thr Pro Leu Ala Asp Asn Asp Asp Val Asp Ala 225 230 235 240 Phe Asp Phe Ile Arg Thr Ile Ala Val Ala Arg Val Met Met Pro Thr 245 250 255 Ser Phe Val Arg Leu Ser Ala Gly Arg Glu Gln Met Asn Glu Gln Thr 260 265 270 Gln Ala Met Cys Phe Met Ala Gly Ala Asn Ser Ile Phe Tyr Gly Cys 275 280 285 Lys Leu Leu Thr Thr Pro Asn Pro Glu Glu Asp Lys Asp Val Gln Leu 290 295 300 Phe Arg Lys Leu Gly Leu Asn Pro Gln Gln Thr Ala Val Leu Thr Gly 305 310 315 320 Asp Asn Glu Gln Gln His Gln Leu Glu Gln Gln Leu Ile Asn Ala Asp 325 330 335 Thr Asp Gln Phe Tyr Asn Ala Ala Thr Val 340 345 <210> 68 <211> 1113 <212> DNA <213> Chromohalobacter salexigens DSM 3043 <220> <221> CDS <222> (1)..(1113) <223> bioB gene encoding biotin synthase from Chromohalobacter salexigens DSM 3043 <400> 68 atg acc gca cag agc cgt gat ccg gca tgg acc gat gca agc ccg acc 48 Met Thr Ala Gln Ser Arg Asp Pro Ala Trp Thr Asp Ala Ser Pro Thr 1 5 10 15 ttt cag ccg acc atg cgt cat gat tgg agc ctg gaa gaa att gaa gca 96 Phe Gln Pro Thr Met Arg His Asp Trp Ser Leu Glu Glu Ile Glu Ala 20 25 30 ctg ttt gca ctg ccg ttt aat gat ctg ctg ttt cgt gca cag cag gtt 144 Leu Phe Ala Leu Pro Phe Asn Asp Leu Leu Phe Arg Ala Gln Gln Val 35 40 45 cat cgt gca cat ttt gat ccg aat gca gtt cag gtt agc acc ctg ctg 192 His Arg Ala His Phe Asp Pro Asn Ala Val Gln Val Ser Thr Leu Leu 50 55 60 agc att aaa acc ggt gca tgt ccg gaa gat tgt aaa tat tgt ccg cag 240 Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys Tyr Cys Pro Gln 65 70 75 80 agc ggt cat tat aat acc ggc ctg ggt aaa gaa aaa ctg ctg gaa att 288 Ser Gly His Tyr Asn Thr Gly Leu Gly Lys Glu Lys Leu Leu Glu Ile 85 90 95 gaa aaa gtt gtt gaa cag gcc cgt gca gca aaa gca gca ggc gca agc 336 Glu Lys Val Val Glu Gln Ala Arg Ala Ala Lys Ala Ala Gly Ala Ser 100 105 110 cgt ttt tgt atg ggt gca gca tgg cgt agc ccg cgt gaa aaa gat ctg 384 Arg Phe Cys Met Gly Ala Ala Trp Arg Ser Pro Arg Glu Lys Asp Leu 115 120 125 cgt gtt gtt acc gaa atg gtt ggt cgt gtt aaa gca ctg ggc ctg gaa 432 Arg Val Val Thr Glu Met Val Gly Arg Val Lys Ala Leu Gly Leu Glu 130 135 140 acc tgt atg acc ctg ggt atg gtt gat gtt gat cag gca cgt cgt ctg 480 Thr Cys Met Thr Leu Gly Met Val Asp Val Asp Gln Ala Arg Arg Leu 145 150 155 160 gca gaa gcc ggt ctg gat tat tat aat cat aat ctg gat acc agc ccg 528 Ala Glu Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu Asp Thr Ser Pro 165 170 175 gat tat tat ggt gaa att att acc acc cgt acc tat gca gat cgt ctg 576 Asp Tyr Tyr Gly Glu Ile Ile Thr Thr Arg Thr Tyr Ala Asp Arg Leu 180 185 190 gaa acc ctg gcc aat gtt cgt gaa gca ggt atg aaa gtt tgt agt ggt 624 Glu Thr Leu Ala Asn Val Arg Glu Ala Gly Met Lys Val Cys Ser Gly 195 200 205 ggt att ctg ggt atg ggt gaa gca ccg cgt gat cgc gca gca ctg ctg 672 Gly Ile Leu Gly Met Gly Glu Ala Pro Arg Asp Arg Ala Ala Leu Leu 210 215 220 cag cag ctg gtt cgt ctg gat ccg cat ccg gaa agc gtt ccg att aat 720 Gln Gln Leu Val Arg Leu Asp Pro His Pro Glu Ser Val Pro Ile Asn 225 230 235 240 atg ctg gtt aaa gtt ccg ggt acc ccg atg gaa aat gtt gaa gat atg 768 Met Leu Val Lys Val Pro Gly Thr Pro Met Glu Asn Val Glu Asp Met 245 250 255 gat ccg ctg acc ttt att cgt gca att gca gtt gca cgt att ctg atg 816 Asp Pro Leu Thr Phe Ile Arg Ala Ile Ala Val Ala Arg Ile Leu Met 260 265 270 ccg aaa agc cat gtt cgt ctg agc gca ggt cgt gaa cag atg gat gaa 864 Pro Lys Ser His Val Arg Leu Ser Ala Gly Arg Glu Gln Met Asp Glu 275 280 285 agc acc cag gca ctg gca ttt ctg gcc ggt gca aat agc att ttt tat 912 Ser Thr Gln Ala Leu Ala Phe Leu Ala Gly Ala Asn Ser Ile Phe Tyr 290 295 300 ggt gat acc ctg ctg acc acc ggt aat ccg cag gtt gaa cgt gat cgt 960 Gly Asp Thr Leu Leu Thr Thr Gly Asn Pro Gln Val Glu Arg Asp Arg 305 310 315 320 gcc ctg ttt gat aaa ctg ggt ctg cat ccg gaa ccg agc gat ccg cat 1008 Ala Leu Phe Asp Lys Leu Gly Leu His Pro Glu Pro Ser Asp Pro His 325 330 335 gca gat gat gcc cat cgt gat gat gaa cag gca gaa att gca ctg gca 1056 Ala Asp Asp Ala His Arg Asp Asp Glu Gln Ala Glu Ile Ala Leu Ala 340 345 350 cat gca att cag cgt cag cgt gat gat gca ctg ttt tat gat gca acc 1104 His Ala Ile Gln Arg Gln Arg Asp Asp Ala Leu Phe Tyr Asp Ala Thr 355 360 365 cgt ggt taa 1113 Arg Gly 370 <210> 69 <211> 370 <212> PRT <213> Chromohalobacter salexigens DSM 3043 <400> 69 Met Thr Ala Gln Ser Arg Asp Pro Ala Trp Thr Asp Ala Ser Pro Thr 1 5 10 15 Phe Gln Pro Thr Met Arg His Asp Trp Ser Leu Glu Glu Ile Glu Ala 20 25 30 Leu Phe Ala Leu Pro Phe Asn Asp Leu Leu Phe Arg Ala Gln Gln Val 35 40 45 His Arg Ala His Phe Asp Pro Asn Ala Val Gln Val Ser Thr Leu Leu 50 55 60 Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys Tyr Cys Pro Gln 65 70 75 80 Ser Gly His Tyr Asn Thr Gly Leu Gly Lys Glu Lys Leu Leu Glu Ile 85 90 95 Glu Lys Val Val Glu Gln Ala Arg Ala Ala Lys Ala Ala Gly Ala Ser 100 105 110 Arg Phe Cys Met Gly Ala Ala Trp Arg Ser Pro Arg Glu Lys Asp Leu 115 120 125 Arg Val Val Thr Glu Met Val Gly Arg Val Lys Ala Leu Gly Leu Glu 130 135 140 Thr Cys Met Thr Leu Gly Met Val Asp Val Asp Gln Ala Arg Arg Leu 145 150 155 160 Ala Glu Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu Asp Thr Ser Pro 165 170 175 Asp Tyr Tyr Gly Glu Ile Ile Thr Thr Arg Thr Tyr Ala Asp Arg Leu 180 185 190 Glu Thr Leu Ala Asn Val Arg Glu Ala Gly Met Lys Val Cys Ser Gly 195 200 205 Gly Ile Leu Gly Met Gly Glu Ala Pro Arg Asp Arg Ala Ala Leu Leu 210 215 220 Gln Gln Leu Val Arg Leu Asp Pro His Pro Glu Ser Val Pro Ile Asn 225 230 235 240 Met Leu Val Lys Val Pro Gly Thr Pro Met Glu Asn Val Glu Asp Met 245 250 255 Asp Pro Leu Thr Phe Ile Arg Ala Ile Ala Val Ala Arg Ile Leu Met 260 265 270 Pro Lys Ser His Val Arg Leu Ser Ala Gly Arg Glu Gln Met Asp Glu 275 280 285 Ser Thr Gln Ala Leu Ala Phe Leu Ala Gly Ala Asn Ser Ile Phe Tyr 290 295 300 Gly Asp Thr Leu Leu Thr Thr Gly Asn Pro Gln Val Glu Arg Asp Arg 305 310 315 320 Ala Leu Phe Asp Lys Leu Gly Leu His Pro Glu Pro Ser Asp Pro His 325 330 335 Ala Asp Asp Ala His Arg Asp Asp Glu Gln Ala Glu Ile Ala Leu Ala 340 345 350 His Ala Ile Gln Arg Gln Arg Asp Asp Ala Leu Phe Tyr Asp Ala Thr 355 360 365 Arg Gly 370 <210> 70 <211> 1059 <212> DNA <213> Pseudomonas caeni <220> <221> CDS <222> (1)..(1059) <223> bioB gene encoding biotin synthase from Pseudomonas caeni <400> 70 atg acc acc agc ccg cat gca gat acc cgt cat gat tgg acc ctg gca 48 Met Thr Thr Ser Pro His Ala Asp Thr Arg His Asp Trp Thr Leu Ala 1 5 10 15 gaa gtt acc gca ctg ctg cag cag ccg ttt aat gat ctg att ttt cag 96 Glu Val Thr Ala Leu Leu Gln Gln Pro Phe Asn Asp Leu Ile Phe Gln 20 25 30 gca cag agc gtt cat cgt cag cat ttt aat gca aat cgt gtt cag gtt 144 Ala Gln Ser Val His Arg Gln His Phe Asn Ala Asn Arg Val Gln Val 35 40 45 agc acc ctg ctg agc att aaa acc ggt gca tgt ccg gaa gat tgt aaa 192 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 tat tgt ccg cag agc ggt cat tat aat acc ggt ctg gat aaa gaa aaa 240 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Asp Lys Glu Lys 65 70 75 80 ctg atg gaa gtg cag aaa gtt ctg gat gaa gcc aaa cgt gcc aaa gaa 288 Leu Met Glu Val Gln Lys Val Leu Asp Glu Ala Lys Arg Ala Lys Glu 85 90 95 att ggt agc acc cgt ttt tgc atg ggt gca gca tgg aaa cat ccg agc 336 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 gca aaa gat ctg ccg tat gtt ctg gaa atg gtt aaa ggt gtt aaa gca 384 Ala Lys Asp Leu Pro Tyr Val Leu Glu Met Val Lys Gly Val Lys Ala 115 120 125 atg ggt atg gaa acc tgt atg acc ctg ggt aaa ctg gat gaa gca cag 432 Met Gly Met Glu Thr Cys Met Thr Leu Gly Lys Leu Asp Glu Ala Gln 130 135 140 acc aaa gcg ctg gca gat gcg ggt ctg gat tat tat aat cat aat ctg 480 Thr Lys Ala Leu Ala Asp Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 gat acc agc ccg gaa ttt tat ggt aat att att acc acc cgt acc tat 528 Asp Thr Ser Pro Glu Phe Tyr Gly Asn Ile Ile Thr Thr Arg Thr Tyr 165 170 175 gca gaa cgt ctg cag acc ctg agc tat gtt cgt gat gca ggt atg aaa 576 Ala Glu Arg Leu Gln Thr Leu Ser Tyr Val Arg Asp Ala Gly Met Lys 180 185 190 att tgt agc ggt ggt att ctg ggt atg ggt gaa agc att gca gat cgt 624 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Ile Ala Asp Arg 195 200 205 gca ggt ctg ctg att cag ctg gca aat ctg ccg gaa cat ccg gaa agc 672 Ala Gly Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 gtt ccg att aat atg ctg gtt aaa gtt gaa ggt acc ccg ctg gaa aat 720 Val Pro Ile Asn Met Leu Val Lys Val Glu Gly Thr Pro Leu Glu Asn 225 230 235 240 gca gaa gat gtt gat ccg ttt gat ttt att cgt atg ctg gca gtt gca 768 Ala Glu Asp Val Asp Pro Phe Asp Phe Ile Arg Met Leu Ala Val Ala 245 250 255 cgc att atg atg ccg aaa agc cat gtt cgt ctg agc gca ggt cgt gaa 816 Arg Ile Met Met Pro Lys Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 cag atg aat gaa cag atg cag agc ctg gca ttt ctg gcc ggt gca aat 864 Gln Met Asn Glu Gln Met Gln Ser Leu Ala Phe Leu Ala Gly Ala Asn 275 280 285 agc att ttt tat ggt gaa aaa ctg ctg acc acc gca aat ccg cag gca 912 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Ala Asn Pro Gln Ala 290 295 300 gat aaa gat atg cag ctg ttt gca cgt ctg ggt att aaa ccg gaa gca 960 Asp Lys Asp Met Gln Leu Phe Ala Arg Leu Gly Ile Lys Pro Glu Ala 305 310 315 320 cgt gaa gaa tat gca gat gaa gtt cat cag gca gca att gaa cat gca 1008 Arg Glu Glu Tyr Ala Asp Glu Val His Gln Ala Ala Ile Glu His Ala 325 330 335 att att gaa cag cgt gat gcc agc ctg ttt tat gat gca gca acc cat 1056 Ile Ile Glu Gln Arg Asp Ala Ser Leu Phe Tyr Asp Ala Ala Thr His 340 345 350 taa 1059 <210> 71 <211> 352 <212> PRT <213> Pseudomonas caeni <400> 71 Met Thr Thr Ser Pro His Ala Asp Thr Arg His Asp Trp Thr Leu Ala 1 5 10 15 Glu Val Thr Ala Leu Leu Gln Gln Pro Phe Asn Asp Leu Ile Phe Gln 20 25 30 Ala Gln Ser Val His Arg Gln His Phe Asn Ala Asn Arg Val Gln Val 35 40 45 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Asp Lys Glu Lys 65 70 75 80 Leu Met Glu Val Gln Lys Val Leu Asp Glu Ala Lys Arg Ala Lys Glu 85 90 95 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 Ala Lys Asp Leu Pro Tyr Val Leu Glu Met Val Lys Gly Val Lys Ala 115 120 125 Met Gly Met Glu Thr Cys Met Thr Leu Gly Lys Leu Asp Glu Ala Gln 130 135 140 Thr Lys Ala Leu Ala Asp Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 Asp Thr Ser Pro Glu Phe Tyr Gly Asn Ile Ile Thr Thr Arg Thr Tyr 165 170 175 Ala Glu Arg Leu Gln Thr Leu Ser Tyr Val Arg Asp Ala Gly Met Lys 180 185 190 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Ile Ala Asp Arg 195 200 205 Ala Gly Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 Val Pro Ile Asn Met Leu Val Lys Val Glu Gly Thr Pro Leu Glu Asn 225 230 235 240 Ala Glu Asp Val Asp Pro Phe Asp Phe Ile Arg Met Leu Ala Val Ala 245 250 255 Arg Ile Met Met Pro Lys Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 Gln Met Asn Glu Gln Met Gln Ser Leu Ala Phe Leu Ala Gly Ala Asn 275 280 285 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Ala Asn Pro Gln Ala 290 295 300 Asp Lys Asp Met Gln Leu Phe Ala Arg Leu Gly Ile Lys Pro Glu Ala 305 310 315 320 Arg Glu Glu Tyr Ala Asp Glu Val His Gln Ala Ala Ile Glu His Ala 325 330 335 Ile Ile Glu Gln Arg Asp Ala Ser Leu Phe Tyr Asp Ala Ala Thr His 340 345 350 <210> 72 <211> 1059 <212> DNA <213> Pseudomonas monteilii <220> <221> CDS <222> (1)..(1059) <223> bioB gene encoding biotin synthase from Pseudomonas monteilii <400> 72 atg agc gca agc acc att gca acc acc cgt cat gat tgg acc ctg gcc 48 Met Ser Ala Ser Thr Ile Ala Thr Thr Arg His Asp Trp Thr Leu Ala 1 5 10 15 gaa gtt cgt gca ctg ttt cag cag ccg ttt aat gat ctg ctg ttt cag 96 Glu Val Arg Ala Leu Phe Gln Gln Pro Phe Asn Asp Leu Leu Phe Gln 20 25 30 gca cag agc gtt cat cgt gca cat ttt gat gca aat cgt gtt cag gtt 144 Ala Gln Ser Val His Arg Ala His Phe Asp Ala Asn Arg Val Gln Val 35 40 45 agc acc ctg ctg agc att aaa acc ggt gca tgt ccg gaa gat tgt aaa 192 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 tat tgc ccg cag agc ggt cat tat aat acc ggt ctg gaa aaa cag aaa 240 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Glu Lys Gln Lys 65 70 75 80 ctg atg gaa gtt cag aaa gtt ctg gaa gaa gca gca cgt gcc aaa gca 288 Leu Met Glu Val Gln Lys Val Leu Glu Glu Ala Ala Arg Ala Lys Ala 85 90 95 att ggt agc acc cgt ttt tgc atg ggt gca gca tgg aaa cat ccg agc 336 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 gca aaa gat atg ccg tat gtt ctg cag atg gtt cag ggt gtt aaa gca 384 Ala Lys Asp Met Pro Tyr Val Leu Gln Met Val Gln Gly Val Lys Ala 115 120 125 atg ggt ctg gaa acc tgt atg acc ctg ggt cgt ctg gat cgc gaa cag 432 Met Gly Leu Glu Thr Cys Met Thr Leu Gly Arg Leu Asp Arg Glu Gln 130 135 140 acc gca gca ctg gca gaa gca ggt ctg gat tat tat aat cat aat ctg 480 Thr Ala Ala Leu Ala Glu Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 gat acc agc ccg gaa ttt tat ggt agc att att acc acc cgt acc tat 528 Asp Thr Ser Pro Glu Phe Tyr Gly Ser Ile Ile Thr Thr Arg Thr Tyr 165 170 175 gca gaa cgt ctg cag acc ctg gca tat gtt cgt gat gca ggt atg aaa 576 Ala Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Asp Ala Gly Met Lys 180 185 190 att tgt agc ggt ggt att ctg ggt atg ggt gaa agc ctg gat gat cgt 624 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Leu Asp Asp Arg 195 200 205 gca aat ctg ctg att cag ctg gcc aat ctg ccg gaa cat ccg gaa agc 672 Ala Asn Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 gtt ccg att aat atg ctg gtt aaa gtt gcc ggt acc ccg ctg gca aat 720 Val Pro Ile Asn Met Leu Val Lys Val Ala Gly Thr Pro Leu Ala Asn 225 230 235 240 gaa gaa gat gtt gat ccg ttt gat ttt att cgt atg ctg gca gtt gca 768 Glu Glu Asp Val Asp Pro Phe Asp Phe Ile Arg Met Leu Ala Val Ala 245 250 255 cgt att ctg atg ccg cag agc cat gtt cgt ctg agc gca ggt cgt gaa 816 Arg Ile Leu Met Pro Gln Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 cag atg aat gaa cag atg cag gca ctg gca ttt ctg gcc ggt gca aat 864 Gln Met Asn Glu Gln Met Gln Ala Leu Ala Phe Leu Ala Gly Ala Asn 275 280 285 agc att ttt tat ggt gaa aaa ctg ctg acc acc ggt aat ccg cag gca 912 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Gly Asn Pro Gln Ala 290 295 300 gat cgt gat atg cag ctg ttt gca cgt ctg ggt att cag ccg gaa gcc 960 Asp Arg Asp Met Gln Leu Phe Ala Arg Leu Gly Ile Gln Pro Glu Ala 305 310 315 320 ggt gaa ggt cat gca gat gaa gtt cat cag gca gca att gaa cag gca 1008 Gly Glu Gly His Ala Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 gtt att gaa cag cgt aat ggt gaa ctg ttt tat gat gca gtt agc gca 1056 Val Ile Glu Gln Arg Asn Gly Glu Leu Phe Tyr Asp Ala Val Ser Ala 340 345 350 taa 1059 <210> 73 <211> 352 <212> PRT <213> Pseudomonas monteilii <400> 73 Met Ser Ala Ser Thr Ile Ala Thr Thr Arg His Asp Trp Thr Leu Ala 1 5 10 15 Glu Val Arg Ala Leu Phe Gln Gln Pro Phe Asn Asp Leu Leu Phe Gln 20 25 30 Ala Gln Ser Val His Arg Ala His Phe Asp Ala Asn Arg Val Gln Val 35 40 45 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Glu Lys Gln Lys 65 70 75 80 Leu Met Glu Val Gln Lys Val Leu Glu Glu Ala Ala Arg Ala Lys Ala 85 90 95 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 Ala Lys Asp Met Pro Tyr Val Leu Gln Met Val Gln Gly Val Lys Ala 115 120 125 Met Gly Leu Glu Thr Cys Met Thr Leu Gly Arg Leu Asp Arg Glu Gln 130 135 140 Thr Ala Ala Leu Ala Glu Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 Asp Thr Ser Pro Glu Phe Tyr Gly Ser Ile Ile Thr Thr Arg Thr Tyr 165 170 175 Ala Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Asp Ala Gly Met Lys 180 185 190 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Leu Asp Asp Arg 195 200 205 Ala Asn Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 Val Pro Ile Asn Met Leu Val Lys Val Ala Gly Thr Pro Leu Ala Asn 225 230 235 240 Glu Glu Asp Val Asp Pro Phe Asp Phe Ile Arg Met Leu Ala Val Ala 245 250 255 Arg Ile Leu Met Pro Gln Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 Gln Met Asn Glu Gln Met Gln Ala Leu Ala Phe Leu Ala Gly Ala Asn 275 280 285 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Gly Asn Pro Gln Ala 290 295 300 Asp Arg Asp Met Gln Leu Phe Ala Arg Leu Gly Ile Gln Pro Glu Ala 305 310 315 320 Gly Glu Gly His Ala Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 Val Ile Glu Gln Arg Asn Gly Glu Leu Phe Tyr Asp Ala Val Ser Ala 340 345 350 <210> 74 <211> 1059 <212> DNA <213> Pseudomonas massiliensis CB1 <220> <221> CDS <222> (1)..(1059) <223> bioB gene encoding biotin synthase from Pseudomonas massiliensis CB1 <400> 74 atg agc gca agc ctg aat agc ccg ctg cgt cat gat tgg acc ctg agc 48 Met Ser Ala Ser Leu Asn Ser Pro Leu Arg His Asp Trp Thr Leu Ser 1 5 10 15 gaa gtt aaa gcc ctg ttt acc cag ccg ttt aat gat ctg ctg ttt cat 96 Glu Val Lys Ala Leu Phe Thr Gln Pro Phe Asn Asp Leu Leu Phe His 20 25 30 gca atg agc gtt cat cgt gca cat ttt gat ccg aat cag gtt cag gtt 144 Ala Met Ser Val His Arg Ala His Phe Asp Pro Asn Gln Val Gln Val 35 40 45 agc acc ctg ctg agc att aaa acc ggt gca tgt ccg gaa gat tgt aaa 192 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 tat tgt ccg cag agc ggt cat tat aat acc ggc ctg gaa aaa gaa aaa 240 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Glu Lys Glu Lys 65 70 75 80 ctg ctg gaa gtg cag aaa gtg att gaa gaa gca gca cgt gcc aaa gca 288 Leu Leu Glu Val Gln Lys Val Ile Glu Glu Ala Ala Arg Ala Lys Ala 85 90 95 att ggt agc acc cgt ttt tgt atg ggt gca gca tgg aaa cat ccg agc 336 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 gca aaa gat atg ccg tat gtt ctg gaa atg gtt cgt ggt gtt aaa gca 384 Ala Lys Asp Met Pro Tyr Val Leu Glu Met Val Arg Gly Val Lys Ala 115 120 125 ctg ggt ctg gaa acc tgt atg acc ctg ggt cgt ctg gat cgt gat cag 432 Leu Gly Leu Glu Thr Cys Met Thr Leu Gly Arg Leu Asp Arg Asp Gln 130 135 140 acc gtt gca ctg gca gaa gca ggt ctg gat tat tat aat cat aat ctg 480 Thr Val Ala Leu Ala Glu Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 gat acc agc ccg gaa ttt tat ggc aat att att acc acc cgt acc tat 528 Asp Thr Ser Pro Glu Phe Tyr Gly Asn Ile Ile Thr Thr Arg Thr Tyr 165 170 175 ggt gaa cgt ctg cag acc ctg gcc tat gtt cgt gat gca ggt atg aaa 576 Gly Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Asp Ala Gly Met Lys 180 185 190 att tgt agc ggt ggt att ctg ggt atg ggt gaa agc ctg gat gat cgt 624 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Leu Asp Asp Arg 195 200 205 gca ggt ctg ctg att cag ctg gca aat ctg ccg gaa cat ccg gaa agc 672 Ala Gly Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 gtt ccg att aat atg ctg gtt aaa gtt gcc ggt acc ccg ctg gaa aat 720 Val Pro Ile Asn Met Leu Val Lys Val Ala Gly Thr Pro Leu Glu Asn 225 230 235 240 gca gaa gat gtt gat ccg ttt gat ttt att cgt atg ctg gca gtt gca 768 Ala Glu Asp Val Asp Pro Phe Asp Phe Ile Arg Met Leu Ala Val Ala 245 250 255 cgt att ctg atg ccg cgt agc cat gtt cgt ctg agc gca ggt cgt gaa 816 Arg Ile Leu Met Pro Arg Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 cag atg aat gaa cag atg cag gcc ctg gca ttt atg gcc ggt gca aat 864 Gln Met Asn Glu Gln Met Gln Ala Leu Ala Phe Met Ala Gly Ala Asn 275 280 285 agc att ttt tat ggt gaa aaa ctg ctg acc acc gca aat ccg cag gca 912 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Ala Asn Pro Gln Ala 290 295 300 gat aaa gat atg cgt ctg ttt gca cgt ctg ggt att cgt ccg gaa gca 960 Asp Lys Asp Met Arg Leu Phe Ala Arg Leu Gly Ile Arg Pro Glu Ala 305 310 315 320 cgt gaa gaa cat gat gat gaa gtt cat cag gca gca att gaa cag gca 1008 Arg Glu Glu His Asp Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 ctg gtt gaa cag cgt agc ggt gaa ctg ttt tat gat gca gca gca gtt 1056 Leu Val Glu Gln Arg Ser Gly Glu Leu Phe Tyr Asp Ala Ala Ala Val 340 345 350 taa 1059 <210> 75 <211> 352 <212> PRT <213> Pseudomonas massiliensis CB1 <400> 75 Met Ser Ala Ser Leu Asn Ser Pro Leu Arg His Asp Trp Thr Leu Ser 1 5 10 15 Glu Val Lys Ala Leu Phe Thr Gln Pro Phe Asn Asp Leu Leu Phe His 20 25 30 Ala Met Ser Val His Arg Ala His Phe Asp Pro Asn Gln Val Gln Val 35 40 45 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Glu Lys Glu Lys 65 70 75 80 Leu Leu Glu Val Gln Lys Val Ile Glu Glu Ala Ala Arg Ala Lys Ala 85 90 95 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 Ala Lys Asp Met Pro Tyr Val Leu Glu Met Val Arg Gly Val Lys Ala 115 120 125 Leu Gly Leu Glu Thr Cys Met Thr Leu Gly Arg Leu Asp Arg Asp Gln 130 135 140 Thr Val Ala Leu Ala Glu Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 Asp Thr Ser Pro Glu Phe Tyr Gly Asn Ile Ile Thr Thr Arg Thr Tyr 165 170 175 Gly Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Asp Ala Gly Met Lys 180 185 190 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Leu Asp Asp Arg 195 200 205 Ala Gly Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 Val Pro Ile Asn Met Leu Val Lys Val Ala Gly Thr Pro Leu Glu Asn 225 230 235 240 Ala Glu Asp Val Asp Pro Phe Asp Phe Ile Arg Met Leu Ala Val Ala 245 250 255 Arg Ile Leu Met Pro Arg Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 Gln Met Asn Glu Gln Met Gln Ala Leu Ala Phe Met Ala Gly Ala Asn 275 280 285 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Ala Asn Pro Gln Ala 290 295 300 Asp Lys Asp Met Arg Leu Phe Ala Arg Leu Gly Ile Arg Pro Glu Ala 305 310 315 320 Arg Glu Glu His Asp Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 Leu Val Glu Gln Arg Ser Gly Glu Leu Phe Tyr Asp Ala Ala Ala Val 340 345 350 <210> 76 <211> 1059 <212> DNA <213> Pseudomonas putida F1 <220> <221> CDS <222> (1)..(1059) <223> bioB gene encoding biotin synthase from Pseudomonas putida F1 <400> 76 atg agc gca agc acc acc gca acc acc cgt cat gat tgg agc ctg gca 48 Met Ser Ala Ser Thr Thr Ala Thr Thr Arg His Asp Trp Ser Leu Ala 1 5 10 15 gaa gtt aaa gcc ctg ttt cag cag ccg ttt aat gat ctg ctg ttt cag 96 Glu Val Lys Ala Leu Phe Gln Gln Pro Phe Asn Asp Leu Leu Phe Gln 20 25 30 gca cag acc gtt cat cgt gca cat ttt aat ccg aat cgt gtt cag gtt 144 Ala Gln Thr Val His Arg Ala His Phe Asn Pro Asn Arg Val Gln Val 35 40 45 agc acc ctg ctg agc att aaa acc ggt gca tgc ccg gaa gat tgt aaa 192 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 tat tgt ccg cag agc ggt cat tat aat acc ggt ctg gaa aaa cag aaa 240 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Glu Lys Gln Lys 65 70 75 80 ctg atg gaa gtt cag aaa gtt ctg gaa gaa gca gcc cgt gca aaa gca 288 Leu Met Glu Val Gln Lys Val Leu Glu Glu Ala Ala Arg Ala Lys Ala 85 90 95 att ggt agc acc cgt ttt tgc atg ggt gca gca tgg aaa cat ccg agc 336 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 gca aaa gat atg ccg tat gtt ctg gaa atg gtt aaa ggt gtt aaa gca 384 Ala Lys Asp Met Pro Tyr Val Leu Glu Met Val Lys Gly Val Lys Ala 115 120 125 atg ggc ctg gaa acc tgt atg acc ctg ggt aaa ctg gat cag gaa cag 432 Met Gly Leu Glu Thr Cys Met Thr Leu Gly Lys Leu Asp Gln Glu Gln 130 135 140 acc aaa gca ctg gca cat gcg ggt ctg gat tat tat aat cat aat ctg 480 Thr Lys Ala Leu Ala His Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 gat acc agc ccg gaa ttt tat ggc agc att att acc acc cgt acc tat 528 Asp Thr Ser Pro Glu Phe Tyr Gly Ser Ile Ile Thr Thr Arg Thr Tyr 165 170 175 agc gaa cgt ctg cag acc ctg gca tat gtt cgt gat gca ggt atg aaa 576 Ser Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Asp Ala Gly Met Lys 180 185 190 att tgt agc ggt ggt att ctg ggt atg ggt gaa agc ctg gat gat cgt 624 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Leu Asp Asp Arg 195 200 205 gca ggt ctg ctg att cag ctg gca aat ctg ccg gaa cat ccg gaa agc 672 Ala Gly Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 gtt ccg att aat atg ctg gtt aaa gtt gca ggt acc ccg ctg gcc gaa 720 Val Pro Ile Asn Met Leu Val Lys Val Ala Gly Thr Pro Leu Ala Glu 225 230 235 240 gaa gaa gat gtt gat ccg ttt gat ttt att cgt atg ctg gca gtt gca 768 Glu Glu Asp Val Asp Pro Phe Asp Phe Ile Arg Met Leu Ala Val Ala 245 250 255 cgt att ctg atg ccg aaa agc cat gtt cgt ctg agc gca ggt cgt gaa 816 Arg Ile Leu Met Pro Lys Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 cag atg aat gaa cag atg cag gcg ctg gca ttt atg gcc ggt gca aat 864 Gln Met Asn Glu Gln Met Gln Ala Leu Ala Phe Met Ala Gly Ala Asn 275 280 285 agc att ttt tat ggt gaa aaa ctg ctg acc acc gcc aat ccg cag gca 912 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Ala Asn Pro Gln Ala 290 295 300 gat aaa gat atg cag ctg ttt gca cgt ctg ggt att aaa ccg gaa gca 960 Asp Lys Asp Met Gln Leu Phe Ala Arg Leu Gly Ile Lys Pro Glu Ala 305 310 315 320 cgt gaa gaa cat gca gat gaa gtt cat cag gca gca att gaa cag gca 1008 Arg Glu Glu His Ala Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 ctg gtt gaa cag cgt agc agc gaa atg ttt tat gat gca gca acc gca 1056 Leu Val Glu Gln Arg Ser Ser Glu Met Phe Tyr Asp Ala Ala Thr Ala 340 345 350 taa 1059 <210> 77 <211> 352 <212> PRT <213> Pseudomonas putida F1 <400> 77 Met Ser Ala Ser Thr Thr Ala Thr Thr Arg His Asp Trp Ser Leu Ala 1 5 10 15 Glu Val Lys Ala Leu Phe Gln Gln Pro Phe Asn Asp Leu Leu Phe Gln 20 25 30 Ala Gln Thr Val His Arg Ala His Phe Asn Pro Asn Arg Val Gln Val 35 40 45 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Glu Lys Gln Lys 65 70 75 80 Leu Met Glu Val Gln Lys Val Leu Glu Glu Ala Ala Arg Ala Lys Ala 85 90 95 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 Ala Lys Asp Met Pro Tyr Val Leu Glu Met Val Lys Gly Val Lys Ala 115 120 125 Met Gly Leu Glu Thr Cys Met Thr Leu Gly Lys Leu Asp Gln Glu Gln 130 135 140 Thr Lys Ala Leu Ala His Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 Asp Thr Ser Pro Glu Phe Tyr Gly Ser Ile Ile Thr Thr Arg Thr Tyr 165 170 175 Ser Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Asp Ala Gly Met Lys 180 185 190 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Leu Asp Asp Arg 195 200 205 Ala Gly Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 Val Pro Ile Asn Met Leu Val Lys Val Ala Gly Thr Pro Leu Ala Glu 225 230 235 240 Glu Glu Asp Val Asp Pro Phe Asp Phe Ile Arg Met Leu Ala Val Ala 245 250 255 Arg Ile Leu Met Pro Lys Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 Gln Met Asn Glu Gln Met Gln Ala Leu Ala Phe Met Ala Gly Ala Asn 275 280 285 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Ala Asn Pro Gln Ala 290 295 300 Asp Lys Asp Met Gln Leu Phe Ala Arg Leu Gly Ile Lys Pro Glu Ala 305 310 315 320 Arg Glu Glu His Ala Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 Leu Val Glu Gln Arg Ser Ser Glu Met Phe Tyr Asp Ala Ala Thr Ala 340 345 350 <210> 78 <211> 1059 <212> DNA <213> Pseudomonas thermotolerans <220> <221> CDS <222> (1)..(1059) <223> bioB gene encoding biotin synthase from Pseudomonas thermotolerans <400> 78 atg aat gca agc gtt gca gca gca att cgt cat gat tgg acc ctg gca 48 Met Asn Ala Ser Val Ala Ala Ala Ile Arg His Asp Trp Thr Leu Ala 1 5 10 15 gaa gtt aaa gcc ctg ttt gca ctg ccg ttt aat gat ctg ctg tat cag 96 Glu Val Lys Ala Leu Phe Ala Leu Pro Phe Asn Asp Leu Leu Tyr Gln 20 25 30 gca cag acc gtt cat cgt cag tat ttt gat gca aat cgt gtt cag gtt 144 Ala Gln Thr Val His Arg Gln Tyr Phe Asp Ala Asn Arg Val Gln Val 35 40 45 agc acc ctg ctg agc att aaa acc ggt gca tgt ccg gaa gat tgt aaa 192 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 tat tgt ccg cag agc ggt cat tat aat acc ggc ctg gaa aaa cag aaa 240 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Glu Lys Gln Lys 65 70 75 80 ctg atg gaa gtt cag aaa gtt ctg cag gca gca gca gaa gca aaa gca 288 Leu Met Glu Val Gln Lys Val Leu Gln Ala Ala Ala Glu Ala Lys Ala 85 90 95 atg ggt agc acc cgt ttt tgt atg ggt gca gca tgg aaa cat ccg agc 336 Met Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 gca aaa gat ttt ccg tat gtt ctg gaa atg gtt aaa ggt gtt aaa gca 384 Ala Lys Asp Phe Pro Tyr Val Leu Glu Met Val Lys Gly Val Lys Ala 115 120 125 ctg ggt ctg gaa acc tgt atg acc ctg ggt cgt ctg tca cgt gaa cag 432 Leu Gly Leu Glu Thr Cys Met Thr Leu Gly Arg Leu Ser Arg Glu Gln 130 135 140 acc cag gca ctg gcg gaa gcc ggt ctg gat tat tat aat cat aat ctg 480 Thr Gln Ala Leu Ala Glu Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 gat acc agc ccg gaa ttt tat ggt cgt att att acc acc cgt acc tat 528 Asp Thr Ser Pro Glu Phe Tyr Gly Arg Ile Ile Thr Thr Arg Thr Tyr 165 170 175 gca gaa cgt ctg cag acc ctg gca tat gtt cgt gaa gca ggt atg aaa 576 Ala Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Glu Ala Gly Met Lys 180 185 190 att tgt agc ggt ggt att ctg ggt atg ggt gaa agc ctg gat gat cgt 624 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Leu Asp Asp Arg 195 200 205 gca ggt ctg ctg att cag ctg gca aat ctg ccg gaa cat ccg gaa agc 672 Ala Gly Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 gtt ccg att aat atg ctg gtt aaa gtt cag ggt acc ccg ctg gca gat 720 Val Pro Ile Asn Met Leu Val Lys Val Gln Gly Thr Pro Leu Ala Asp 225 230 235 240 gca gaa gat gtt gat ccg ttt gat ttt att cgt acc ctg gcg gtt gca 768 Ala Glu Asp Val Asp Pro Phe Asp Phe Ile Arg Thr Leu Ala Val Ala 245 250 255 cgt att atg atg ccg aaa agc cat gtt cgt ctg agc gca ggt cgc gaa 816 Arg Ile Met Met Pro Lys Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 cag atg aat gaa cag atg cag gcg ctg gca ttt ctg gcc ggt gca aat 864 Gln Met Asn Glu Gln Met Gln Ala Leu Ala Phe Leu Ala Gly Ala Asn 275 280 285 agc att ttt tat ggt gaa aaa ctg ctg acc acc ggt aat ccg cag gca 912 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Gly Asn Pro Gln Ala 290 295 300 gaa aaa gat ctg cag ctg ttt cgt cgt ctg ggt att cag ccg gaa gaa 960 Glu Lys Asp Leu Gln Leu Phe Arg Arg Leu Gly Ile Gln Pro Glu Glu 305 310 315 320 cgt gaa gaa cat gca gat gaa gtt cat cag gcc gca att gaa cag gcc 1008 Arg Glu Glu His Ala Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 ctg gcc gaa cag cgt gat agc cag ctg ttt tat gat gca gca agc gca 1056 Leu Ala Glu Gln Arg Asp Ser Gln Leu Phe Tyr Asp Ala Ala Ser Ala 340 345 350 taa 1059 <210> 79 <211> 352 <212> PRT <213> Pseudomonas thermotolerans <400> 79 Met Asn Ala Ser Val Ala Ala Ala Ile Arg His Asp Trp Thr Leu Ala 1 5 10 15 Glu Val Lys Ala Leu Phe Ala Leu Pro Phe Asn Asp Leu Leu Tyr Gln 20 25 30 Ala Gln Thr Val His Arg Gln Tyr Phe Asp Ala Asn Arg Val Gln Val 35 40 45 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Glu Lys Gln Lys 65 70 75 80 Leu Met Glu Val Gln Lys Val Leu Gln Ala Ala Ala Glu Ala Lys Ala 85 90 95 Met Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 Ala Lys Asp Phe Pro Tyr Val Leu Glu Met Val Lys Gly Val Lys Ala 115 120 125 Leu Gly Leu Glu Thr Cys Met Thr Leu Gly Arg Leu Ser Arg Glu Gln 130 135 140 Thr Gln Ala Leu Ala Glu Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 Asp Thr Ser Pro Glu Phe Tyr Gly Arg Ile Ile Thr Thr Arg Thr Tyr 165 170 175 Ala Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Glu Ala Gly Met Lys 180 185 190 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Leu Asp Asp Arg 195 200 205 Ala Gly Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 Val Pro Ile Asn Met Leu Val Lys Val Gln Gly Thr Pro Leu Ala Asp 225 230 235 240 Ala Glu Asp Val Asp Pro Phe Asp Phe Ile Arg Thr Leu Ala Val Ala 245 250 255 Arg Ile Met Met Pro Lys Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 Gln Met Asn Glu Gln Met Gln Ala Leu Ala Phe Leu Ala Gly Ala Asn 275 280 285 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Gly Asn Pro Gln Ala 290 295 300 Glu Lys Asp Leu Gln Leu Phe Arg Arg Leu Gly Ile Gln Pro Glu Glu 305 310 315 320 Arg Glu Glu His Ala Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 Leu Ala Glu Gln Arg Asp Ser Gln Leu Phe Tyr Asp Ala Ala Ser Ala 340 345 350 <210> 80 <211> 1059 <212> DNA <213> pseudomonad ancestor <220> <221> CDS <222> (1)..(1059) <223> bioB gene encoding biotin synthase from pseudomonad ancestor <400> 80 atg agc gca agc acc aat agc ccg ctg cgt cat gat tgg acc ctg agc 48 Met Ser Ala Ser Thr Asn Ser Pro Leu Arg His Asp Trp Thr Leu Ser 1 5 10 15 gaa gtt aaa gca ctg ttt acc cag ccg ttt aat gat ctg ctg ttt cat 96 Glu Val Lys Ala Leu Phe Thr Gln Pro Phe Asn Asp Leu Leu Phe His 20 25 30 gca atg acc gtt cat cgt gca cat ttt gat ccg aat cag gtt cag gtt 144 Ala Met Thr Val His Arg Ala His Phe Asp Pro Asn Gln Val Gln Val 35 40 45 agc acc ctg ctg agc att aaa acc ggt gca tgt ccg gaa gat tgt aaa 192 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 tat tgt ccg cag agc ggc cat tat aat acc ggc ctg gaa aaa gaa aaa 240 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Glu Lys Glu Lys 65 70 75 80 ctg atg gaa gtg cag aaa gtt att gaa gaa gca gca cgt gcc aaa gca 288 Leu Met Glu Val Gln Lys Val Ile Glu Glu Ala Ala Arg Ala Lys Ala 85 90 95 att ggt agc acc cgt ttt tgc atg ggt gca gca tgg aaa cat ccg agc 336 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 gca aaa gat atg ccg tat gtt ctg gaa atg gtt cgt ggt gtt aaa gca 384 Ala Lys Asp Met Pro Tyr Val Leu Glu Met Val Arg Gly Val Lys Ala 115 120 125 atg ggt ctg gaa acc tgt atg acc ctg ggt cgt ctg gat cag gat cag 432 Met Gly Leu Glu Thr Cys Met Thr Leu Gly Arg Leu Asp Gln Asp Gln 130 135 140 acc gtt gca ctg gca gaa gca ggt ctg gat tat tat aat cat aat ctg 480 Thr Val Ala Leu Ala Glu Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 gat acc agc ccg gaa ttt tat ggt aat att att acc acc cgt acc tat 528 Asp Thr Ser Pro Glu Phe Tyr Gly Asn Ile Ile Thr Thr Arg Thr Tyr 165 170 175 ggt gaa cgt ctg cag acc ctg gcc tat gtt cgt gat gca ggt atg aaa 576 Gly Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Asp Ala Gly Met Lys 180 185 190 att tgt agc ggt ggt att ctg ggt atg ggt gaa agc ctg gat gat cgt 624 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Leu Asp Asp Arg 195 200 205 gca ggt ctg ctg att cag ctg gca aat ctg ccg gaa cat ccg gaa agc 672 Ala Gly Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 gtt ccg att aat atg ctg gtt aaa gtt gca ggt acc ccg ctg gaa aat 720 Val Pro Ile Asn Met Leu Val Lys Val Ala Gly Thr Pro Leu Glu Asn 225 230 235 240 gca gaa gat gtt gat ccg ttt gat ttt att cgt atg ctg gca gtt gca 768 Ala Glu Asp Val Asp Pro Phe Asp Phe Ile Arg Met Leu Ala Val Ala 245 250 255 cgt att ctg atg ccg cgt agc cat gtt cgt ctg agc gca ggt cgt gaa 816 Arg Ile Leu Met Pro Arg Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 cag atg aat gaa cag atg cag gcc ctg gca ttt atg gcc ggt gca aat 864 Gln Met Asn Glu Gln Met Gln Ala Leu Ala Phe Met Ala Gly Ala Asn 275 280 285 agc att ttt tat ggt gaa aaa ctg ctg acc acc gca aat ccg cag gca 912 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Ala Asn Pro Gln Ala 290 295 300 gat aaa gat atg cag ctg ttt gca cgt ctg ggt att cgt ccg gaa gca 960 Asp Lys Asp Met Gln Leu Phe Ala Arg Leu Gly Ile Arg Pro Glu Ala 305 310 315 320 cgt gaa gaa cat gat gat gaa gtt cat cag gca gca att gaa cag gca 1008 Arg Glu Glu His Asp Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 ctg gtt gaa cag cgt agc agc gaa atg ttt tat gat gca gca gca gtt 1056 Leu Val Glu Gln Arg Ser Ser Glu Met Phe Tyr Asp Ala Ala Ala Val 340 345 350 taa 1059 <210> 81 <211> 352 <212> PRT <213> pseudomonad ancestor <400> 81 Met Ser Ala Ser Thr Asn Ser Pro Leu Arg His Asp Trp Thr Leu Ser 1 5 10 15 Glu Val Lys Ala Leu Phe Thr Gln Pro Phe Asn Asp Leu Leu Phe His 20 25 30 Ala Met Thr Val His Arg Ala His Phe Asp Pro Asn Gln Val Gln Val 35 40 45 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Glu Lys Glu Lys 65 70 75 80 Leu Met Glu Val Gln Lys Val Ile Glu Glu Ala Ala Arg Ala Lys Ala 85 90 95 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 Ala Lys Asp Met Pro Tyr Val Leu Glu Met Val Arg Gly Val Lys Ala 115 120 125 Met Gly Leu Glu Thr Cys Met Thr Leu Gly Arg Leu Asp Gln Asp Gln 130 135 140 Thr Val Ala Leu Ala Glu Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 Asp Thr Ser Pro Glu Phe Tyr Gly Asn Ile Ile Thr Thr Arg Thr Tyr 165 170 175 Gly Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Asp Ala Gly Met Lys 180 185 190 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Leu Asp Asp Arg 195 200 205 Ala Gly Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 Val Pro Ile Asn Met Leu Val Lys Val Ala Gly Thr Pro Leu Glu Asn 225 230 235 240 Ala Glu Asp Val Asp Pro Phe Asp Phe Ile Arg Met Leu Ala Val Ala 245 250 255 Arg Ile Leu Met Pro Arg Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 Gln Met Asn Glu Gln Met Gln Ala Leu Ala Phe Met Ala Gly Ala Asn 275 280 285 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Ala Asn Pro Gln Ala 290 295 300 Asp Lys Asp Met Gln Leu Phe Ala Arg Leu Gly Ile Arg Pro Glu Ala 305 310 315 320 Arg Glu Glu His Asp Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 Leu Val Glu Gln Arg Ser Ser Glu Met Phe Tyr Asp Ala Ala Ala Val 340 345 350 <210> 82 <211> 1059 <212> DNA <213> Pseudomonas aeruginosa PAO1 <220> <221> CDS <222> (1)..(1059) <223> bioB gene encoding biotin synthase from Pseudomonas aeruginosa PAO1 <400> 82 atg agc gca acc gca agc gtt gca acc cgt cat gat tgg agc ctg gca 48 Met Ser Ala Thr Ala Ser Val Ala Thr Arg His Asp Trp Ser Leu Ala 1 5 10 15 gaa gtt cgt gca ctg ttt gaa cag ccg ttt aat gat ctg ctg ttt cag 96 Glu Val Arg Ala Leu Phe Glu Gln Pro Phe Asn Asp Leu Leu Phe Gln 20 25 30 gca cag acc gtt cat cgt gca cat ttt gat ccg aat cgt gtt cag gtt 144 Ala Gln Thr Val His Arg Ala His Phe Asp Pro Asn Arg Val Gln Val 35 40 45 agc acc ctg ctg agc att aaa acc ggt gca tgt ccg gaa gat tgt aaa 192 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 tat tgt ccg cag agc ggt cat tat aat acc ggc ctg gat aaa gaa aaa 240 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Asp Lys Glu Lys 65 70 75 80 ctg atg gaa gtt cag aaa gtt ctg gaa gca gca gca gaa gca aaa gca 288 Leu Met Glu Val Gln Lys Val Leu Glu Ala Ala Ala Glu Ala Lys Ala 85 90 95 att ggt agc acc cgt ttt tgc atg ggt gca gca tgg aaa cat ccg agc 336 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 gca aaa gat atg ccg tat gtt ctg gaa atg gtt aaa ggt gtt aaa aaa 384 Ala Lys Asp Met Pro Tyr Val Leu Glu Met Val Lys Gly Val Lys Lys 115 120 125 ctg ggt ctg gaa acc tgt atg acc ctg ggt cgt ctg acc cag gaa cag 432 Leu Gly Leu Glu Thr Cys Met Thr Leu Gly Arg Leu Thr Gln Glu Gln 130 135 140 acc cag gcg ctg gca gat gcc ggt ctg gat tat tat aat cat aat ctg 480 Thr Gln Ala Leu Ala Asp Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 gat acc agc ccg gaa ttt tat ggt aat att att acc acc cgt acc tat 528 Asp Thr Ser Pro Glu Phe Tyr Gly Asn Ile Ile Thr Thr Arg Thr Tyr 165 170 175 agc gaa cgt ctg cag acc ctg gcc tat gtt cgt gaa gca ggt atg aaa 576 Ser Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Glu Ala Gly Met Lys 180 185 190 att tgt agc ggt ggt att ctg ggt atg ggt gaa agc gtt gat gat cgt 624 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Val Asp Asp Arg 195 200 205 gca ggt ctg ctg att cag ctg gca aat ctg ccg gaa cat ccg gaa agc 672 Ala Gly Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 gtt ccg att aat atg ctg gtt aaa gtt aaa ggt acc ccg ctg gcc gaa 720 Val Pro Ile Asn Met Leu Val Lys Val Lys Gly Thr Pro Leu Ala Glu 225 230 235 240 gaa aaa gat gtt gat ccg ttt gat ttt att cgt acc ctg gca gtt gcc 768 Glu Lys Asp Val Asp Pro Phe Asp Phe Ile Arg Thr Leu Ala Val Ala 245 250 255 cgt att atg atg ccg aaa agc cat gtt cgt ctg agc gca ggt cgt gaa 816 Arg Ile Met Met Pro Lys Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 cag atg aat gaa cag atg cag gca ctg gca ttt atg gcc ggt gca aat 864 Gln Met Asn Glu Gln Met Gln Ala Leu Ala Phe Met Ala Gly Ala Asn 275 280 285 agc att ttt tat ggt gaa aaa ctg ctg acc acc aaa aat ccg cag gcc 912 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Lys Asn Pro Gln Ala 290 295 300 gaa aaa gat atg cag ctg ttt gca cgt ctg ggt att aaa ccg gaa gaa 960 Glu Lys Asp Met Gln Leu Phe Ala Arg Leu Gly Ile Lys Pro Glu Glu 305 310 315 320 cgt gaa gaa cat gca gat gaa gtt cat cag gca gca att gaa cag gcc 1008 Arg Glu Glu His Ala Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 ctg gtt gaa cag cgt gaa agc aaa ctg ttt tat aat gca gca agc gca 1056 Leu Val Glu Gln Arg Glu Ser Lys Leu Phe Tyr Asn Ala Ala Ser Ala 340 345 350 taa 1059 <210> 83 <211> 352 <212> PRT <213> Pseudomonas aeruginosa PAO1 <400> 83 Met Ser Ala Thr Ala Ser Val Ala Thr Arg His Asp Trp Ser Leu Ala 1 5 10 15 Glu Val Arg Ala Leu Phe Glu Gln Pro Phe Asn Asp Leu Leu Phe Gln 20 25 30 Ala Gln Thr Val His Arg Ala His Phe Asp Pro Asn Arg Val Gln Val 35 40 45 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Asp Lys Glu Lys 65 70 75 80 Leu Met Glu Val Gln Lys Val Leu Glu Ala Ala Ala Glu Ala Lys Ala 85 90 95 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 Ala Lys Asp Met Pro Tyr Val Leu Glu Met Val Lys Gly Val Lys Lys 115 120 125 Leu Gly Leu Glu Thr Cys Met Thr Leu Gly Arg Leu Thr Gln Glu Gln 130 135 140 Thr Gln Ala Leu Ala Asp Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 Asp Thr Ser Pro Glu Phe Tyr Gly Asn Ile Ile Thr Thr Arg Thr Tyr 165 170 175 Ser Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Glu Ala Gly Met Lys 180 185 190 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Val Asp Asp Arg 195 200 205 Ala Gly Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 Val Pro Ile Asn Met Leu Val Lys Val Lys Gly Thr Pro Leu Ala Glu 225 230 235 240 Glu Lys Asp Val Asp Pro Phe Asp Phe Ile Arg Thr Leu Ala Val Ala 245 250 255 Arg Ile Met Met Pro Lys Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 Gln Met Asn Glu Gln Met Gln Ala Leu Ala Phe Met Ala Gly Ala Asn 275 280 285 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Lys Asn Pro Gln Ala 290 295 300 Glu Lys Asp Met Gln Leu Phe Ala Arg Leu Gly Ile Lys Pro Glu Glu 305 310 315 320 Arg Glu Glu His Ala Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 Leu Val Glu Gln Arg Glu Ser Lys Leu Phe Tyr Asn Ala Ala Ser Ala 340 345 350 <210> 84 <211> 1059 <212> DNA <213> Pseudomonas balearica <220> <221> CDS <222> (1)..(1059) <223> bioB gene encoding biotin synthase from Pseudomonas balearica <400> 84 atg agc gca acc gca agc att gca acc cgt cat gat tgg agc ctg gcg 48 Met Ser Ala Thr Ala Ser Ile Ala Thr Arg His Asp Trp Ser Leu Ala 1 5 10 15 gaa gtt aaa gca ctg ttt gaa cag ccg ttt aat gat ctg ctg ttt cag 96 Glu Val Lys Ala Leu Phe Glu Gln Pro Phe Asn Asp Leu Leu Phe Gln 20 25 30 gca cag acc gtt cat cgt gca cat ttt gat ccg aat cgt gtt cag gtt 144 Ala Gln Thr Val His Arg Ala His Phe Asp Pro Asn Arg Val Gln Val 35 40 45 agc acc ctg ctg agc att aaa acc ggt gca tgt ccg gaa gat tgc aaa 192 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 tat tgt ccg cag agc ggt cat tat aat acc ggt ctg gat aaa gaa aaa 240 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Asp Lys Glu Lys 65 70 75 80 ctg atg gaa gtt cag aaa gtt ctg gaa gca gca gca gaa gcc aaa gca 288 Leu Met Glu Val Gln Lys Val Leu Glu Ala Ala Ala Glu Ala Lys Ala 85 90 95 att ggt agc acc cgt ttt tgc atg ggt gca gca tgg aaa cat ccg agc 336 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 gca aaa gat atg ccg tat gtt ctg gaa atg gtt aaa ggt gtt aaa aaa 384 Ala Lys Asp Met Pro Tyr Val Leu Glu Met Val Lys Gly Val Lys Lys 115 120 125 ctg ggt ctg gaa acc tgt atg acc ctg ggt cgt ctg gat cag gaa cag 432 Leu Gly Leu Glu Thr Cys Met Thr Leu Gly Arg Leu Asp Gln Glu Gln 130 135 140 acc cag gcc ctg gca gaa gca ggc ctg gat tat tat aat cat aat ctg 480 Thr Gln Ala Leu Ala Glu Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 gat acc agc ccg gaa ttt tat ggt aat att att acc acc cgt acc tat 528 Asp Thr Ser Pro Glu Phe Tyr Gly Asn Ile Ile Thr Thr Arg Thr Tyr 165 170 175 agc gaa cgc ctg cag acc ctg gca tat gtt cgt gaa gca ggt atg aaa 576 Ser Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Glu Ala Gly Met Lys 180 185 190 att tgt agc ggt ggt att ctg ggt atg ggt gaa agc gtt gat gat cgt 624 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Val Asp Asp Arg 195 200 205 gca ggt ctg ctg att cag ctg gca aat ctg ccg gaa cat ccg gaa agc 672 Ala Gly Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 gtt ccg att aat atg ctg gtt aaa gtg aaa ggt acc ccg ctg gcc gaa 720 Val Pro Ile Asn Met Leu Val Lys Val Lys Gly Thr Pro Leu Ala Glu 225 230 235 240 gaa aaa gat gtt gat ccg ttt gat ttt att cgt acc ctg gcc gtt gca 768 Glu Lys Asp Val Asp Pro Phe Asp Phe Ile Arg Thr Leu Ala Val Ala 245 250 255 cgt att atg atg ccg aaa agc cat gtt cgt ctg agc gca ggt cgt gaa 816 Arg Ile Met Met Pro Lys Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 cag atg aat gaa cag atg cag gcg ctg gca ttt atg gcc ggt gca aat 864 Gln Met Asn Glu Gln Met Gln Ala Leu Ala Phe Met Ala Gly Ala Asn 275 280 285 agc att ttt tat ggt gaa aaa ctg ctg acc acc gca aat ccg cag gca 912 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Ala Asn Pro Gln Ala 290 295 300 gaa aaa gat atg cag ctg ttt gca cgt ctg ggt att aaa ccg gaa gaa 960 Glu Lys Asp Met Gln Leu Phe Ala Arg Leu Gly Ile Lys Pro Glu Glu 305 310 315 320 cgt gaa gaa cat gca gat gaa gtt cat cag gca gca att gaa cag gca 1008 Arg Glu Glu His Ala Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 ctg gtt gaa cag cgt gat agc cag ctg ttt tat gat gca gca agc gca 1056 Leu Val Glu Gln Arg Asp Ser Gln Leu Phe Tyr Asp Ala Ala Ser Ala 340 345 350 taa 1059 <210> 85 <211> 352 <212> PRT <213> Pseudomonas balearica <400> 85 Met Ser Ala Thr Ala Ser Ile Ala Thr Arg His Asp Trp Ser Leu Ala 1 5 10 15 Glu Val Lys Ala Leu Phe Glu Gln Pro Phe Asn Asp Leu Leu Phe Gln 20 25 30 Ala Gln Thr Val His Arg Ala His Phe Asp Pro Asn Arg Val Gln Val 35 40 45 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Asp Lys Glu Lys 65 70 75 80 Leu Met Glu Val Gln Lys Val Leu Glu Ala Ala Ala Glu Ala Lys Ala 85 90 95 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 Ala Lys Asp Met Pro Tyr Val Leu Glu Met Val Lys Gly Val Lys Lys 115 120 125 Leu Gly Leu Glu Thr Cys Met Thr Leu Gly Arg Leu Asp Gln Glu Gln 130 135 140 Thr Gln Ala Leu Ala Glu Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 Asp Thr Ser Pro Glu Phe Tyr Gly Asn Ile Ile Thr Thr Arg Thr Tyr 165 170 175 Ser Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Glu Ala Gly Met Lys 180 185 190 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Val Asp Asp Arg 195 200 205 Ala Gly Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 Val Pro Ile Asn Met Leu Val Lys Val Lys Gly Thr Pro Leu Ala Glu 225 230 235 240 Glu Lys Asp Val Asp Pro Phe Asp Phe Ile Arg Thr Leu Ala Val Ala 245 250 255 Arg Ile Met Met Pro Lys Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 Gln Met Asn Glu Gln Met Gln Ala Leu Ala Phe Met Ala Gly Ala Asn 275 280 285 Ser Ile Phe Tyr Gly Glu Lys Leu Leu Thr Thr Ala Asn Pro Gln Ala 290 295 300 Glu Lys Asp Met Gln Leu Phe Ala Arg Leu Gly Ile Lys Pro Glu Glu 305 310 315 320 Arg Glu Glu His Ala Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 Leu Val Glu Gln Arg Asp Ser Gln Leu Phe Tyr Asp Ala Ala Ser Ala 340 345 350 <210> 86 <211> 1056 <212> DNA <213> Pseudomonas fluorescens SBW25 <220> <221> CDS <222> (1)..(1056) <223> bioB gene encoding biotin synthase from Pseudomonas fluorescens SBW25 <400> 86 atg agc gca agc acc acc gcc acc ctg cgt cat gat tgg agc ctg gca 48 Met Ser Ala Ser Thr Thr Ala Thr Leu Arg His Asp Trp Ser Leu Ala 1 5 10 15 gaa gtt aaa gca ctg ttt gtt cag ccg ttt aat gat ctg ctg ttt cag 96 Glu Val Lys Ala Leu Phe Val Gln Pro Phe Asn Asp Leu Leu Phe Gln 20 25 30 gca cag acc gtt cat cgt gca cat ttt gat gca aat cgt gtt cag gtt 144 Ala Gln Thr Val His Arg Ala His Phe Asp Ala Asn Arg Val Gln Val 35 40 45 agc acc ctg ctg agc att aaa acc ggt gca tgt ccg gaa gat tgt aaa 192 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 tat tgt ccg cag agc ggt cat tat aat acc ggc ctg gaa aaa gaa aaa 240 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Glu Lys Glu Lys 65 70 75 80 ctg atg gaa gtt cag aaa gtt ctg gaa gaa gca gca cgt gca aaa gca 288 Leu Met Glu Val Gln Lys Val Leu Glu Glu Ala Ala Arg Ala Lys Ala 85 90 95 att ggt agc acc cgt ttt tgc atg ggt gca gca tgg aaa cat ccg agc 336 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 gca aaa gat atg ccg tat gtt ctg cag atg gtt aaa ggt gtt aaa gca 384 Ala Lys Asp Met Pro Tyr Val Leu Gln Met Val Lys Gly Val Lys Ala 115 120 125 atg ggt ctg gaa acc tgt atg acc ctg ggt cgt ctg gat cag gat cag 432 Met Gly Leu Glu Thr Cys Met Thr Leu Gly Arg Leu Asp Gln Asp Gln 130 135 140 acc gaa gca ctg gca cag gca ggt ctg gat tat tat aat cat aat ctg 480 Thr Glu Ala Leu Ala Gln Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 gat acc agc ccg gaa ttt tat ggt agc att att acc acc cgt acc tat 528 Asp Thr Ser Pro Glu Phe Tyr Gly Ser Ile Ile Thr Thr Arg Thr Tyr 165 170 175 ggt gaa cgt ctg cag acc ctg gca tat gtt cgt gat agc ggt atg aaa 576 Gly Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Asp Ser Gly Met Lys 180 185 190 att tgt agc ggt ggt att ctg ggt atg ggt gaa agc ctg gat gat cgt 624 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Leu Asp Asp Arg 195 200 205 gca aat ctg ctg att cag ctg gcc aat ctg ccg gaa cat ccg gaa agc 672 Ala Asn Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 gtt ccg att aat atg ctg gtt aaa gtt gca ggt acc ccg ctg gaa aat 720 Val Pro Ile Asn Met Leu Val Lys Val Ala Gly Thr Pro Leu Glu Asn 225 230 235 240 gcc gaa gat att gat ccg ttt gat ttt att cgt atg ctg gca gtt gca 768 Ala Glu Asp Ile Asp Pro Phe Asp Phe Ile Arg Met Leu Ala Val Ala 245 250 255 cgt att ctg atg ccg cgt agc cat gtt cgt ctg agc gca ggt cgt gaa 816 Arg Ile Leu Met Pro Arg Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 gca atg aat gaa cag atg cag gca ctg gcc ttt ttt gcc ggt gca aat 864 Ala Met Asn Glu Gln Met Gln Ala Leu Ala Phe Phe Ala Gly Ala Asn 275 280 285 agc att ttt tat ggt gat aaa ctg ctg acc acc gca aat ccg cag gca 912 Ser Ile Phe Tyr Gly Asp Lys Leu Leu Thr Thr Ala Asn Pro Gln Ala 290 295 300 gat aaa gat atg cag ctg ttt agc cgt ctg ggt att ctg ccg gaa gca 960 Asp Lys Asp Met Gln Leu Phe Ser Arg Leu Gly Ile Leu Pro Glu Ala 305 310 315 320 cgt gaa gaa cat gca gat gaa gtt cat cag gca gca att gaa cag gcc 1008 Arg Glu Glu His Ala Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 ctg gtt gaa cag aaa agc agc gaa cag ttt tat aat gca gca gtt taa 1056 Leu Val Glu Gln Lys Ser Ser Glu Gln Phe Tyr Asn Ala Ala Val 340 345 350 <210> 87 <211> 351 <212> PRT <213> Pseudomonas fluorescens SBW25 <400> 87 Met Ser Ala Ser Thr Thr Ala Thr Leu Arg His Asp Trp Ser Leu Ala 1 5 10 15 Glu Val Lys Ala Leu Phe Val Gln Pro Phe Asn Asp Leu Leu Phe Gln 20 25 30 Ala Gln Thr Val His Arg Ala His Phe Asp Ala Asn Arg Val Gln Val 35 40 45 Ser Thr Leu Leu Ser Ile Lys Thr Gly Ala Cys Pro Glu Asp Cys Lys 50 55 60 Tyr Cys Pro Gln Ser Gly His Tyr Asn Thr Gly Leu Glu Lys Glu Lys 65 70 75 80 Leu Met Glu Val Gln Lys Val Leu Glu Glu Ala Ala Arg Ala Lys Ala 85 90 95 Ile Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Lys His Pro Ser 100 105 110 Ala Lys Asp Met Pro Tyr Val Leu Gln Met Val Lys Gly Val Lys Ala 115 120 125 Met Gly Leu Glu Thr Cys Met Thr Leu Gly Arg Leu Asp Gln Asp Gln 130 135 140 Thr Glu Ala Leu Ala Gln Ala Gly Leu Asp Tyr Tyr Asn His Asn Leu 145 150 155 160 Asp Thr Ser Pro Glu Phe Tyr Gly Ser Ile Ile Thr Thr Arg Thr Tyr 165 170 175 Gly Glu Arg Leu Gln Thr Leu Ala Tyr Val Arg Asp Ser Gly Met Lys 180 185 190 Ile Cys Ser Gly Gly Ile Leu Gly Met Gly Glu Ser Leu Asp Asp Arg 195 200 205 Ala Asn Leu Leu Ile Gln Leu Ala Asn Leu Pro Glu His Pro Glu Ser 210 215 220 Val Pro Ile Asn Met Leu Val Lys Val Ala Gly Thr Pro Leu Glu Asn 225 230 235 240 Ala Glu Asp Ile Asp Pro Phe Asp Phe Ile Arg Met Leu Ala Val Ala 245 250 255 Arg Ile Leu Met Pro Arg Ser His Val Arg Leu Ser Ala Gly Arg Glu 260 265 270 Ala Met Asn Glu Gln Met Gln Ala Leu Ala Phe Phe Ala Gly Ala Asn 275 280 285 Ser Ile Phe Tyr Gly Asp Lys Leu Leu Thr Thr Ala Asn Pro Gln Ala 290 295 300 Asp Lys Asp Met Gln Leu Phe Ser Arg Leu Gly Ile Leu Pro Glu Ala 305 310 315 320 Arg Glu Glu His Ala Asp Glu Val His Gln Ala Ala Ile Glu Gln Ala 325 330 335 Leu Val Glu Gln Lys Ser Ser Glu Gln Phe Tyr Asn Ala Ala Val 340 345 350 <210> 88 <211> 756 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(756) <223> bioC gene encoding SAM (S-adenosylmethionine)-dependent methyltransferase (BioC) <400> 88 atg gca acg gtt aat aaa caa gcc att gca gcg gca ttt ggt cgg gca 48 Met Ala Thr Val Asn Lys Gln Ala Ile Ala Ala Ala Phe Gly Arg Ala 1 5 10 15 gcc gca cac tat gag caa cat gca gat cta cag cgc cag agt gct gac 96 Ala Ala His Tyr Glu Gln His Ala Asp Leu Gln Arg Gln Ser Ala Asp 20 25 30 gcc tta ctg gca atg ctt cca cag cgt aaa tac acc cac gta ctg gac 144 Ala Leu Leu Ala Met Leu Pro Gln Arg Lys Tyr Thr His Val Leu Asp 35 40 45 gcg ggt tgt gga cct ggc tgg atg agc cgc cac tgg cgg gaa cgt cac 192 Ala Gly Cys Gly Pro Gly Trp Met Ser Arg His Trp Arg Glu Arg His 50 55 60 gcg cag gtg acg gcc tta gat ctc tcg ccg cca atg ctt gtt cag gca 240 Ala Gln Val Thr Ala Leu Asp Leu Ser Pro Pro Met Leu Val Gln Ala 65 70 75 80 cgc cag aag gat gcc gca gac cat tat ctg gcg gga gat atc gaa tcc 288 Arg Gln Lys Asp Ala Ala Asp His Tyr Leu Ala Gly Asp Ile Glu Ser 85 90 95 ctg ccg tta gcg act gcg acg ttc gat ctt gca tgg agc aat ctc gca 336 Leu Pro Leu Ala Thr Ala Thr Phe Asp Leu Ala Trp Ser Asn Leu Ala 100 105 110 gtg cag tgg tgc ggt aat tta tcc acg gca ctc cgc gag ctg tat cgg 384 Val Gln Trp Cys Gly Asn Leu Ser Thr Ala Leu Arg Glu Leu Tyr Arg 115 120 125 gtg gtg cgc ccc aaa ggc gtg gtc gcg ttt acc acg ctg gtg cag gga 432 Val Val Arg Pro Lys Gly Val Val Ala Phe Thr Thr Leu Val Gln Gly 130 135 140 tcg tta ccc gaa ctg cat cag gcg tgg cag gcg gtg gac gag cgt ccg 480 Ser Leu Pro Glu Leu His Gln Ala Trp Gln Ala Val Asp Glu Arg Pro 145 150 155 160 cat gct aat cgc ttt tta ccg cca gat gaa atc gaa cag tcg ctg aac 528 His Ala Asn Arg Phe Leu Pro Pro Asp Glu Ile Glu Gln Ser Leu Asn 165 170 175 ggc gtg cat tat caa cat cat att cag ccc atc acg ctg tgg ttt gat 576 Gly Val His Tyr Gln His His Ile Gln Pro Ile Thr Leu Trp Phe Asp 180 185 190 gat gcg ctc agt gcc atg cgt tcg ctg aaa ggc atc ggt gcc acg cat 624 Asp Ala Leu Ser Ala Met Arg Ser Leu Lys Gly Ile Gly Ala Thr His 195 200 205 ctt cat gaa ggg cgc gac ccg cga ata tta acg cgt tcg cag ttg cag 672 Leu His Glu Gly Arg Asp Pro Arg Ile Leu Thr Arg Ser Gln Leu Gln 210 215 220 cga ttg caa ctg gcc tgg ccg caa cag cag ggg cga tat cct ctg acg 720 Arg Leu Gln Leu Ala Trp Pro Gln Gln Gln Gly Arg Tyr Pro Leu Thr 225 230 235 240 tat cat ctt ttt ttg gga gtg att gct cgt gag taa 756 Tyr His Leu Phe Leu Gly Val Ile Ala Arg Glu 245 250 <210> 89 <211> 251 <212> PRT <213> Escherichia coli <400> 89 Met Ala Thr Val Asn Lys Gln Ala Ile Ala Ala Ala Phe Gly Arg Ala 1 5 10 15 Ala Ala His Tyr Glu Gln His Ala Asp Leu Gln Arg Gln Ser Ala Asp 20 25 30 Ala Leu Leu Ala Met Leu Pro Gln Arg Lys Tyr Thr His Val Leu Asp 35 40 45 Ala Gly Cys Gly Pro Gly Trp Met Ser Arg His Trp Arg Glu Arg His 50 55 60 Ala Gln Val Thr Ala Leu Asp Leu Ser Pro Pro Met Leu Val Gln Ala 65 70 75 80 Arg Gln Lys Asp Ala Ala Asp His Tyr Leu Ala Gly Asp Ile Glu Ser 85 90 95 Leu Pro Leu Ala Thr Ala Thr Phe Asp Leu Ala Trp Ser Asn Leu Ala 100 105 110 Val Gln Trp Cys Gly Asn Leu Ser Thr Ala Leu Arg Glu Leu Tyr Arg 115 120 125 Val Val Arg Pro Lys Gly Val Val Ala Phe Thr Thr Leu Val Gln Gly 130 135 140 Ser Leu Pro Glu Leu His Gln Ala Trp Gln Ala Val Asp Glu Arg Pro 145 150 155 160 His Ala Asn Arg Phe Leu Pro Pro Asp Glu Ile Glu Gln Ser Leu Asn 165 170 175 Gly Val His Tyr Gln His His Ile Gln Pro Ile Thr Leu Trp Phe Asp 180 185 190 Asp Ala Leu Ser Ala Met Arg Ser Leu Lys Gly Ile Gly Ala Thr His 195 200 205 Leu His Glu Gly Arg Asp Pro Arg Ile Leu Thr Arg Ser Gln Leu Gln 210 215 220 Arg Leu Gln Leu Ala Trp Pro Gln Gln Gln Gly Arg Tyr Pro Leu Thr 225 230 235 240 Tyr His Leu Phe Leu Gly Val Ile Ala Arg Glu 245 250 <210> 90 <211> 1155 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(1155) <223> bioF gene encoding (Bio F) 7-keto-8-aminopelargonic acid (KAPA) synthase <400> 90 atg agc tgg cag gag aaa atc aac gcg gcg ctc gat gcg cgg cgt gct 48 Met Ser Trp Gln Glu Lys Ile Asn Ala Ala Leu Asp Ala Arg Arg Ala 1 5 10 15 gcc gat gcc ctg cgt cgc cgt tat ccg gtg gcg caa gga gcc gga cgc 96 Ala Asp Ala Leu Arg Arg Arg Tyr Pro Val Ala Gln Gly Ala Gly Arg 20 25 30 tgg ctg gtg gcg gat gat cgc cag tat ctg aac ttt tcc agt aac gat 144 Trp Leu Val Ala Asp Asp Arg Gln Tyr Leu Asn Phe Ser Ser Asn Asp 35 40 45 tat tta ggt tta agc cat cat ccg caa att atc cgt gcc tgg cag cag 192 Tyr Leu Gly Leu Ser His His Pro Gln Ile Ile Arg Ala Trp Gln Gln 50 55 60 ggg gcg gag caa ttt ggc atc ggt agc ggc ggc tcc ggt cac gtc agc 240 Gly Ala Glu Gln Phe Gly Ile Gly Ser Gly Gly Ser Gly His Val Ser 65 70 75 80 ggt tat agc gtg gtg cat cag gca ctg gaa gaa gag ctg gcc gag tgg 288 Gly Tyr Ser Val Val His Gln Ala Leu Glu Glu Glu Leu Ala Glu Trp 85 90 95 ctt ggc tat tcg cgg gca ctg ctg ttt atc tct ggt ttc gcc gct aat 336 Leu Gly Tyr Ser Arg Ala Leu Leu Phe Ile Ser Gly Phe Ala Ala Asn 100 105 110 cag gca gtt att gcc gcg atg atg gcg aaa gag gac cgt att gct gcc 384 Gln Ala Val Ile Ala Ala Met Met Ala Lys Glu Asp Arg Ile Ala Ala 115 120 125 gac cgg ctt agc cat gcc tca ttg ctg gaa gct gcc agt tta agc ccg 432 Asp Arg Leu Ser His Ala Ser Leu Leu Glu Ala Ala Ser Leu Ser Pro 130 135 140 tcg cag ctt cgc cgt ttt gct cat aac gat gtc act cat ttg gcg cga 480 Ser Gln Leu Arg Arg Phe Ala His Asn Asp Val Thr His Leu Ala Arg 145 150 155 160 ttg ctt gct tcc ccc tgt ccg ggg cag caa atg gtg gtg aca gaa ggc 528 Leu Leu Ala Ser Pro Cys Pro Gly Gln Gln Met Val Val Thr Glu Gly 165 170 175 gtg ttc agc atg gac ggc gat agt gcg cca ctg gcg gaa atc cag cag 576 Val Phe Ser Met Asp Gly Asp Ser Ala Pro Leu Ala Glu Ile Gln Gln 180 185 190 gta acg caa cag cac aat ggc tgg ttg atg gtc gat gat gcc cac ggc 624 Val Thr Gln Gln His Asn Gly Trp Leu Met Val Asp Asp Ala His Gly 195 200 205 acg ggc gtt atc ggg gag cag ggg cgc ggc agc tgc tgg ctg caa aag 672 Thr Gly Val Ile Gly Glu Gln Gly Arg Gly Ser Cys Trp Leu Gln Lys 210 215 220 gta aaa cca gaa ttg ctg gta gtg act ttt ggc aaa gga ttt ggc gtc 720 Val Lys Pro Glu Leu Leu Val Val Thr Phe Gly Lys Gly Phe Gly Val 225 230 235 240 agc ggg gca gcg gtg ctt tgc tcc agt acg gtg gcg gat tat ctg ctg 768 Ser Gly Ala Ala Val Leu Cys Ser Ser Thr Val Ala Asp Tyr Leu Leu 245 250 255 caa ttc gcc cgc cac ctt atc tac agc acc agt atg ccg ccc gct cag 816 Gln Phe Ala Arg His Leu Ile Tyr Ser Thr Ser Met Pro Pro Ala Gln 260 265 270 gcg cag gca tta cgt gcg tcg ctg gcg gtc att cgc agt gat gag ggt 864 Ala Gln Ala Leu Arg Ala Ser Leu Ala Val Ile Arg Ser Asp Glu Gly 275 280 285 gat gca cgg cgc gaa aaa ctg gcg gca ctc att acg cgt ttt cgt gcc 912 Asp Ala Arg Arg Glu Lys Leu Ala Ala Leu Ile Thr Arg Phe Arg Ala 290 295 300 gga gta cag gat ttg ccg ttt acg ctt gct gat tca tgc agc gcc atc 960 Gly Val Gln Asp Leu Pro Phe Thr Leu Ala Asp Ser Cys Ser Ala Ile 305 310 315 320 cag cca ttg att gtc ggt gat aac agc cgt gcg tta caa ctg gca gaa 1008 Gln Pro Leu Ile Val Gly Asp Asn Ser Arg Ala Leu Gln Leu Ala Glu 325 330 335 aaa ctg cgt cag caa ggc tgc tgg gtc acg gcg att cgc ccg cca acc 1056 Lys Leu Arg Gln Gln Gly Cys Trp Val Thr Ala Ile Arg Pro Pro Thr 340 345 350 gta ccc gct ggt act gcg cga ctg cgc tta acg cta acc gct gcg cat 1104 Val Pro Ala Gly Thr Ala Arg Leu Arg Leu Thr Leu Thr Ala Ala His 355 360 365 gaa atg cag gat atc gac cgt ctg ctg gag gtg ctg cat ggc aac ggt 1152 Glu Met Gln Asp Ile Asp Arg Leu Leu Glu Val Leu His Gly Asn Gly 370 375 380 taa 1155 <210> 91 <211> 384 <212> PRT <213> Escherichia coli <400> 91 Met Ser Trp Gln Glu Lys Ile Asn Ala Ala Leu Asp Ala Arg Arg Ala 1 5 10 15 Ala Asp Ala Leu Arg Arg Arg Tyr Pro Val Ala Gln Gly Ala Gly Arg 20 25 30 Trp Leu Val Ala Asp Asp Arg Gln Tyr Leu Asn Phe Ser Ser Asn Asp 35 40 45 Tyr Leu Gly Leu Ser His His Pro Gln Ile Ile Arg Ala Trp Gln Gln 50 55 60 Gly Ala Glu Gln Phe Gly Ile Gly Ser Gly Gly Ser Gly His Val Ser 65 70 75 80 Gly Tyr Ser Val Val His Gln Ala Leu Glu Glu Glu Leu Ala Glu Trp 85 90 95 Leu Gly Tyr Ser Arg Ala Leu Leu Phe Ile Ser Gly Phe Ala Ala Asn 100 105 110 Gln Ala Val Ile Ala Ala Met Met Ala Lys Glu Asp Arg Ile Ala Ala 115 120 125 Asp Arg Leu Ser His Ala Ser Leu Leu Glu Ala Ala Ser Leu Ser Pro 130 135 140 Ser Gln Leu Arg Arg Phe Ala His Asn Asp Val Thr His Leu Ala Arg 145 150 155 160 Leu Leu Ala Ser Pro Cys Pro Gly Gln Gln Met Val Val Thr Glu Gly 165 170 175 Val Phe Ser Met Asp Gly Asp Ser Ala Pro Leu Ala Glu Ile Gln Gln 180 185 190 Val Thr Gln Gln His Asn Gly Trp Leu Met Val Asp Asp Ala His Gly 195 200 205 Thr Gly Val Ile Gly Glu Gln Gly Arg Gly Ser Cys Trp Leu Gln Lys 210 215 220 Val Lys Pro Glu Leu Leu Val Val Thr Phe Gly Lys Gly Phe Gly Val 225 230 235 240 Ser Gly Ala Ala Val Leu Cys Ser Ser Thr Val Ala Asp Tyr Leu Leu 245 250 255 Gln Phe Ala Arg His Leu Ile Tyr Ser Thr Ser Met Pro Pro Ala Gln 260 265 270 Ala Gln Ala Leu Arg Ala Ser Leu Ala Val Ile Arg Ser Asp Glu Gly 275 280 285 Asp Ala Arg Arg Glu Lys Leu Ala Ala Leu Ile Thr Arg Phe Arg Ala 290 295 300 Gly Val Gln Asp Leu Pro Phe Thr Leu Ala Asp Ser Cys Ser Ala Ile 305 310 315 320 Gln Pro Leu Ile Val Gly Asp Asn Ser Arg Ala Leu Gln Leu Ala Glu 325 330 335 Lys Leu Arg Gln Gln Gly Cys Trp Val Thr Ala Ile Arg Pro Pro Thr 340 345 350 Val Pro Ala Gly Thr Ala Arg Leu Arg Leu Thr Leu Thr Ala Ala His 355 360 365 Glu Met Gln Asp Ile Asp Arg Leu Leu Glu Val Leu His Gly Asn Gly 370 375 380 <210> 92 <211> 1290 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(1290) <223> bioA gene encoding 7,8-Diaminopelargonic Acid (DAPA) Synthase (BioA) <400> 92 atg aca acg gac gat ctt gcc ttt gac caa cgc cat atc tgg cac cca 48 Met Thr Thr Asp Asp Leu Ala Phe Asp Gln Arg His Ile Trp His Pro 1 5 10 15 tac aca tcc atg acc tcc cct ctg ccg gtt tat ccg gtg gtg agc gcc 96 Tyr Thr Ser Met Thr Ser Pro Leu Pro Val Tyr Pro Val Val Ser Ala 20 25 30 gaa ggt tgc gag ctg att ttg tct gac ggc aga cgc ctg gtt gac ggt 144 Glu Gly Cys Glu Leu Ile Leu Ser Asp Gly Arg Arg Leu Val Asp Gly 35 40 45 atg tcg tcc tgg tgg gcg gcg atc cac ggc tac aat cac ccg cag ctt 192 Met Ser Ser Trp Trp Ala Ala Ile His Gly Tyr Asn His Pro Gln Leu 50 55 60 aat gcg gcg atg aag tcg caa att gat gcc atg tcg cat gtg atg ttt 240 Asn Ala Ala Met Lys Ser Gln Ile Asp Ala Met Ser His Val Met Phe 65 70 75 80 ggc ggt atc acc cat gcg cca gcc att gag ctg tgc cgc aaa ctg gtg 288 Gly Gly Ile Thr His Ala Pro Ala Ile Glu Leu Cys Arg Lys Leu Val 85 90 95 gcg atg acg ccg caa ccg ctg gag tgc gtt ttt ctc gcg gac tcc ggt 336 Ala Met Thr Pro Gln Pro Leu Glu Cys Val Phe Leu Ala Asp Ser Gly 100 105 110 tcc gta gcg gtg gaa gtg gcg atg aaa atg gcg ttg cag tac tgg caa 384 Ser Val Ala Val Glu Val Ala Met Lys Met Ala Leu Gln Tyr Trp Gln 115 120 125 gcc aaa ggc gaa gcg cgc cag cgt ttt ctg acc ttc cgc aat ggt tat 432 Ala Lys Gly Glu Ala Arg Gln Arg Phe Leu Thr Phe Arg Asn Gly Tyr 130 135 140 cat ggc gat acc ttt ggc gcg atg tcg gtg tgc gat ccg gat aac tca 480 His Gly Asp Thr Phe Gly Ala Met Ser Val Cys Asp Pro Asp Asn Ser 145 150 155 160 atg cac agt ctg tgg aaa ggc tac ctg cca gaa aac ctg ttt gct ccc 528 Met His Ser Leu Trp Lys Gly Tyr Leu Pro Glu Asn Leu Phe Ala Pro 165 170 175 gcc ccg caa agc cgc atg gat ggc gaa tgg gat gag cgc gat atg gtg 576 Ala Pro Gln Ser Arg Met Asp Gly Glu Trp Asp Glu Arg Asp Met Val 180 185 190 ggc ttt gcc cgc ctg atg gcg gcg cat cgt cat gaa atc gcg gcg gtg 624 Gly Phe Ala Arg Leu Met Ala Ala His Arg His Glu Ile Ala Ala Val 195 200 205 atc att gag ccg att gtc cag ggc gca ggc ggg atg cgc atg tac cat 672 Ile Ile Glu Pro Ile Val Gln Gly Ala Gly Gly Met Arg Met Tyr His 210 215 220 ccg gaa tgg tta aaa cga atc cgc aaa ata tgc gat cgc gaa ggt atc 720 Pro Glu Trp Leu Lys Arg Ile Arg Lys Ile Cys Asp Arg Glu Gly Ile 225 230 235 240 ttg ctg att gcc gac gag atc gcc act gga ttt ggt cgt acc ggg aaa 768 Leu Leu Ile Ala Asp Glu Ile Ala Thr Gly Phe Gly Arg Thr Gly Lys 245 250 255 ctg ttt gcc tgt gaa cat gca gaa atc gcg ccg gac att ttg tgc ctc 816 Leu Phe Ala Cys Glu His Ala Glu Ile Ala Pro Asp Ile Leu Cys Leu 260 265 270 ggt aaa gcc tta acc ggc ggc aca atg acc ctt tcc gcc aca ctc acc 864 Gly Lys Ala Leu Thr Gly Gly Thr Met Thr Leu Ser Ala Thr Leu Thr 275 280 285 acg cgc gag gtt gca gaa acc atc agt aac ggt gaa gcc ggt tgc ttt 912 Thr Arg Glu Val Ala Glu Thr Ile Ser Asn Gly Glu Ala Gly Cys Phe 290 295 300 atg cat ggg cca act ttt atg ggc aat ccg ctg gcc tgc gcg gca gca 960 Met His Gly Pro Thr Phe Met Gly Asn Pro Leu Ala Cys Ala Ala Ala 305 310 315 320 aac gcc agc ctg gcg att ctc gaa tct ggc gac tgg cag caa cag gtg 1008 Asn Ala Ser Leu Ala Ile Leu Glu Ser Gly Asp Trp Gln Gln Gln Val 325 330 335 gcg gat att gaa gta cag ctg cgc gag caa ctt gcc ccc gcc cgt gat 1056 Ala Asp Ile Glu Val Gln Leu Arg Glu Gln Leu Ala Pro Ala Arg Asp 340 345 350 gcc gaa atg gtt gcc gat gtg cgc gta ctg ggg gcc att ggc gtg gtc 1104 Ala Glu Met Val Ala Asp Val Arg Val Leu Gly Ala Ile Gly Val Val 355 360 365 gaa acc act cat ccg gtg aat atg gcg gcg ctg caa aaa ttc ttt gtc 1152 Glu Thr Thr His Pro Val Asn Met Ala Ala Leu Gln Lys Phe Phe Val 370 375 380 gaa cag ggt gtc tgg atc cgg cct ttt ggc aaa ctg att tac ctg atg 1200 Glu Gln Gly Val Trp Ile Arg Pro Phe Gly Lys Leu Ile Tyr Leu Met 385 390 395 400 ccg ccc tat att att ctc ccg caa cag ttg cag cgt ctg acc gca gcg 1248 Pro Pro Tyr Ile Ile Leu Pro Gln Gln Leu Gln Arg Leu Thr Ala Ala 405 410 415 gtt aac cgc gcg gta cag gat gaa aca ttt ttt tgc caa taa 1290 Val Asn Arg Ala Val Gln Asp Glu Thr Phe Phe Cys Gln 420 425 <210> 93 <211> 429 <212> PRT <213> Escherichia coli <400> 93 Met Thr Thr Asp Asp Leu Ala Phe Asp Gln Arg His Ile Trp His Pro 1 5 10 15 Tyr Thr Ser Met Thr Ser Pro Leu Pro Val Tyr Pro Val Val Ser Ala 20 25 30 Glu Gly Cys Glu Leu Ile Leu Ser Asp Gly Arg Arg Leu Val Asp Gly 35 40 45 Met Ser Ser Trp Trp Ala Ala Ile His Gly Tyr Asn His Pro Gln Leu 50 55 60 Asn Ala Ala Met Lys Ser Gln Ile Asp Ala Met Ser His Val Met Phe 65 70 75 80 Gly Gly Ile Thr His Ala Pro Ala Ile Glu Leu Cys Arg Lys Leu Val 85 90 95 Ala Met Thr Pro Gln Pro Leu Glu Cys Val Phe Leu Ala Asp Ser Gly 100 105 110 Ser Val Ala Val Glu Val Ala Met Lys Met Ala Leu Gln Tyr Trp Gln 115 120 125 Ala Lys Gly Glu Ala Arg Gln Arg Phe Leu Thr Phe Arg Asn Gly Tyr 130 135 140 His Gly Asp Thr Phe Gly Ala Met Ser Val Cys Asp Pro Asp Asn Ser 145 150 155 160 Met His Ser Leu Trp Lys Gly Tyr Leu Pro Glu Asn Leu Phe Ala Pro 165 170 175 Ala Pro Gln Ser Arg Met Asp Gly Glu Trp Asp Glu Arg Asp Met Val 180 185 190 Gly Phe Ala Arg Leu Met Ala Ala His Arg His Glu Ile Ala Ala Val 195 200 205 Ile Ile Glu Pro Ile Val Gln Gly Ala Gly Gly Met Arg Met Tyr His 210 215 220 Pro Glu Trp Leu Lys Arg Ile Arg Lys Ile Cys Asp Arg Glu Gly Ile 225 230 235 240 Leu Leu Ile Ala Asp Glu Ile Ala Thr Gly Phe Gly Arg Thr Gly Lys 245 250 255 Leu Phe Ala Cys Glu His Ala Glu Ile Ala Pro Asp Ile Leu Cys Leu 260 265 270 Gly Lys Ala Leu Thr Gly Gly Thr Met Thr Leu Ser Ala Thr Leu Thr 275 280 285 Thr Arg Glu Val Ala Glu Thr Ile Ser Asn Gly Glu Ala Gly Cys Phe 290 295 300 Met His Gly Pro Thr Phe Met Gly Asn Pro Leu Ala Cys Ala Ala Ala 305 310 315 320 Asn Ala Ser Leu Ala Ile Leu Glu Ser Gly Asp Trp Gln Gln Gln Val 325 330 335 Ala Asp Ile Glu Val Gln Leu Arg Glu Gln Leu Ala Pro Ala Arg Asp 340 345 350 Ala Glu Met Val Ala Asp Val Arg Val Leu Gly Ala Ile Gly Val Val 355 360 365 Glu Thr Thr His Pro Val Asn Met Ala Ala Leu Gln Lys Phe Phe Val 370 375 380 Glu Gln Gly Val Trp Ile Arg Pro Phe Gly Lys Leu Ile Tyr Leu Met 385 390 395 400 Pro Pro Tyr Ile Ile Leu Pro Gln Gln Leu Gln Arg Leu Thr Ala Ala 405 410 415 Val Asn Arg Ala Val Gln Asp Glu Thr Phe Phe Cys Gln 420 425 <210> 94 <211> 678 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(678) <223> bioD gene encoding Dethiobiotin (DTB) Synthetase (BioD) <400> 94 gtg agt aaa cgt tat ttt gtc acc gga acg gat acc gaa gtg ggg aaa 48 Val Ser Lys Arg Tyr Phe Val Thr Gly Thr Asp Thr Glu Val Gly Lys 1 5 10 15 act gtc gcc agt tgt gca ctt tta caa gcc gca aag gca gca ggc tac 96 Thr Val Ala Ser Cys Ala Leu Leu Gln Ala Ala Lys Ala Ala Gly Tyr 20 25 30 cgg acg gca ggt tat aaa ccg gtc gcc tct ggc agc gaa aag acc ccg 144 Arg Thr Ala Gly Tyr Lys Pro Val Ala Ser Gly Ser Glu Lys Thr Pro 35 40 45 gaa ggt tta cgc aat agc gac gcg ctg gcg tta cag cgc aac agc agc 192 Glu Gly Leu Arg Asn Ser Asp Ala Leu Ala Leu Gln Arg Asn Ser Ser 50 55 60 ctg cag ctg gat tac gca aca gta aat cct tac acc ttc gca gaa ccc 240 Leu Gln Leu Asp Tyr Ala Thr Val Asn Pro Tyr Thr Phe Ala Glu Pro 65 70 75 80 act tcg ccg cac atc atc agc gcg caa gag ggc aga ccg ata gaa tca 288 Thr Ser Pro His Ile Ile Ser Ala Gln Glu Gly Arg Pro Ile Glu Ser 85 90 95 ttg gta atg agc gcc gga tta cgc gcg ctt gaa caa cag gct gac tgg 336 Leu Val Met Ser Ala Gly Leu Arg Ala Leu Glu Gln Gln Ala Asp Trp 100 105 110 gtg tta gtg gaa ggt gct ggc ggc tgg ttt acg ccg ctt tct gac act 384 Val Leu Val Glu Gly Ala Gly Gly Trp Phe Thr Pro Leu Ser Asp Thr 115 120 125 ttc act ttt gca gat tgg gta aca cag gaa caa ctg ccg gtg ata ctg 432 Phe Thr Phe Ala Asp Trp Val Thr Gln Glu Gln Leu Pro Val Ile Leu 130 135 140 gta gtt ggt gtg aaa ctc ggc tgt att aat cac gcg atg ttg act gca 480 Val Val Gly Val Lys Leu Gly Cys Ile Asn His Ala Met Leu Thr Ala 145 150 155 160 cag gta ata caa cac gcc gga ctg act ctg gcg ggt tgg gtg gcg aac 528 Gln Val Ile Gln His Ala Gly Leu Thr Leu Ala Gly Trp Val Ala Asn 165 170 175 gat gtt acg cct ccg gga aaa cgt cac gct gaa tat atg acc acg ctc 576 Asp Val Thr Pro Pro Gly Lys Arg His Ala Glu Tyr Met Thr Thr Leu 180 185 190 acc cgc atg att ccc gcg ccg ctg ctg gga gag atc ccc tgg ctt gca 624 Thr Arg Met Ile Pro Ala Pro Leu Leu Gly Glu Ile Pro Trp Leu Ala 195 200 205 gaa aat cca gaa aat gcg gca acc gga aag tac ata aac ctt gcc ttg 672 Glu Asn Pro Glu Asn Ala Ala Thr Gly Lys Tyr Ile Asn Leu Ala Leu 210 215 220 ttg tag 678 Leu 225 <210> 95 <211> 225 <212> PRT <213> Escherichia coli <400> 95 Val Ser Lys Arg Tyr Phe Val Thr Gly Thr Asp Thr Glu Val Gly Lys 1 5 10 15 Thr Val Ala Ser Cys Ala Leu Leu Gln Ala Ala Lys Ala Ala Gly Tyr 20 25 30 Arg Thr Ala Gly Tyr Lys Pro Val Ala Ser Gly Ser Glu Lys Thr Pro 35 40 45 Glu Gly Leu Arg Asn Ser Asp Ala Leu Ala Leu Gln Arg Asn Ser Ser 50 55 60 Leu Gln Leu Asp Tyr Ala Thr Val Asn Pro Tyr Thr Phe Ala Glu Pro 65 70 75 80 Thr Ser Pro His Ile Ile Ser Ala Gln Glu Gly Arg Pro Ile Glu Ser 85 90 95 Leu Val Met Ser Ala Gly Leu Arg Ala Leu Glu Gln Gln Ala Asp Trp 100 105 110 Val Leu Val Glu Gly Ala Gly Gly Trp Phe Thr Pro Leu Ser Asp Thr 115 120 125 Phe Thr Phe Ala Asp Trp Val Thr Gln Glu Gln Leu Pro Val Ile Leu 130 135 140 Val Val Gly Val Lys Leu Gly Cys Ile Asn His Ala Met Leu Thr Ala 145 150 155 160 Gln Val Ile Gln His Ala Gly Leu Thr Leu Ala Gly Trp Val Ala Asn 165 170 175 Asp Val Thr Pro Pro Gly Lys Arg His Ala Glu Tyr Met Thr Thr Leu 180 185 190 Thr Arg Met Ile Pro Ala Pro Leu Leu Gly Glu Ile Pro Trp Leu Ala 195 200 205 Glu Asn Pro Glu Asn Ala Ala Thr Gly Lys Tyr Ile Asn Leu Ala Leu 210 215 220 Leu 225 <210> 96 <211> 1347 <212> DNA <213> Bacillus subtilis <220> <221> CDS <222> (1)..(1347) <223> bioK gene encoding L-lysine:8-amino-7-oxononanoate aminotransferase (BioK) <400> 96 atg act cac gat tta atc gaa aaa agc aaa aag cac ttg tgg ctg ccc 48 Met Thr His Asp Leu Ile Glu Lys Ser Lys Lys His Leu Trp Leu Pro 1 5 10 15 ttc act cag atg aaa gat tat gac gaa aac cct ttg atc att gaa agc 96 Phe Thr Gln Met Lys Asp Tyr Asp Glu Asn Pro Leu Ile Ile Glu Ser 20 25 30 ggc aca ggg att aaa gta aaa gat atc aat ggg aag gaa tat tac gac 144 Gly Thr Gly Ile Lys Val Lys Asp Ile Asn Gly Lys Glu Tyr Tyr Asp 35 40 45 ggc ttc agt tct gtt tgg ctg aac gtt cac ggg cac cgc aag aag gag 192 Gly Phe Ser Ser Val Trp Leu Asn Val His Gly His Arg Lys Lys Glu 50 55 60 ctt gat gac gca atc aag aag caa tta ggt aag att gcg cat agt acg 240 Leu Asp Asp Ala Ile Lys Lys Gln Leu Gly Lys Ile Ala His Ser Thr 65 70 75 80 ctt tta gga atg acg aat gtc cct gca act caa tta gct gaa aca ctt 288 Leu Leu Gly Met Thr Asn Val Pro Ala Thr Gln Leu Ala Glu Thr Leu 85 90 95 att gat att tcc cct aaa aag ctg acc cgt gtt ttt tat tct gat tcc 336 Ile Asp Ile Ser Pro Lys Lys Leu Thr Arg Val Phe Tyr Ser Asp Ser 100 105 110 ggt gca gaa gct atg gag att gcg ctt aaa atg gcc ttt cag tat tgg 384 Gly Ala Glu Ala Met Glu Ile Ala Leu Lys Met Ala Phe Gln Tyr Trp 115 120 125 aaa aat att ggc aaa cca gaa aag caa aaa ttc atc gcc atg aag aat 432 Lys Asn Ile Gly Lys Pro Glu Lys Gln Lys Phe Ile Ala Met Lys Asn 130 135 140 gga tac cac ggt gat acc atc gga gca gta agc gta ggc tca att gag 480 Gly Tyr His Gly Asp Thr Ile Gly Ala Val Ser Val Gly Ser Ile Glu 145 150 155 160 ttg ttt cac cac gta tat ggt cca ttg atg ttt gag tct tac aag gcg 528 Leu Phe His His Val Tyr Gly Pro Leu Met Phe Glu Ser Tyr Lys Ala 165 170 175 cct att ccc tat gtt tac cgc tcg gag tca ggt gac cca gat gag tgc 576 Pro Ile Pro Tyr Val Tyr Arg Ser Glu Ser Gly Asp Pro Asp Glu Cys 180 185 190 cgc gac cag tgc ctt cgc gaa ttg gcc cag ctt ttg gag gaa cac cat 624 Arg Asp Gln Cys Leu Arg Glu Leu Ala Gln Leu Leu Glu Glu His His 195 200 205 gag gag atc gcg gca ctg agt att gaa tca atg gtt caa ggg gcg agt 672 Glu Glu Ile Ala Ala Leu Ser Ile Glu Ser Met Val Gln Gly Ala Ser 210 215 220 gga atg att gta atg cca gaa ggc tac tta gca ggc gta cgc gaa ctt 720 Gly Met Ile Val Met Pro Glu Gly Tyr Leu Ala Gly Val Arg Glu Leu 225 230 235 240 tgc acg act tac gat gtc ttg atg att gtg gat gaa gtt gca aca gga 768 Cys Thr Thr Tyr Asp Val Leu Met Ile Val Asp Glu Val Ala Thr Gly 245 250 255 ttc ggt cgc acc ggg aaa atg ttt gca tgc gaa cat gaa aac gtg caa 816 Phe Gly Arg Thr Gly Lys Met Phe Ala Cys Glu His Glu Asn Val Gln 260 265 270 ccg gat ctg atg gcc gca ggc aag ggt atc acg ggc gga tac ctt ccg 864 Pro Asp Leu Met Ala Ala Gly Lys Gly Ile Thr Gly Gly Tyr Leu Pro 275 280 285 att gcg gtt act ttt gcc acc gaa gac att tat aag gca ttt tac gat 912 Ile Ala Val Thr Phe Ala Thr Glu Asp Ile Tyr Lys Ala Phe Tyr Asp 290 295 300 gat tat gaa aac ttg aag acc ttt ttt cat gga cac tct tac aca gga 960 Asp Tyr Glu Asn Leu Lys Thr Phe Phe His Gly His Ser Tyr Thr Gly 305 310 315 320 aat caa ctg ggt tgt gca gtc gca ctg gag aat ctg gca ctg ttt gaa 1008 Asn Gln Leu Gly Cys Ala Val Ala Leu Glu Asn Leu Ala Leu Phe Glu 325 330 335 agc gaa aac att gtt gag cag gtc gct gaa aaa tcg aag aaa tta cat 1056 Ser Glu Asn Ile Val Glu Gln Val Ala Glu Lys Ser Lys Lys Leu His 340 345 350 ttt tta tta caa gat tta cat gcc ttg cca cat gta ggc gac atc cgt 1104 Phe Leu Leu Gln Asp Leu His Ala Leu Pro His Val Gly Asp Ile Arg 355 360 365 caa tta gga ttc atg tgt ggt gcg gag tta gtc cgt agc aaa gaa aca 1152 Gln Leu Gly Phe Met Cys Gly Ala Glu Leu Val Arg Ser Lys Glu Thr 370 375 380 aaa gag ccc tat ccc gct gat cgt cgc atc ggt tac aaa gtc agt ctt 1200 Lys Glu Pro Tyr Pro Ala Asp Arg Arg Ile Gly Tyr Lys Val Ser Leu 385 390 395 400 aaa atg cgt gaa tta ggg atg ttg aca cgc ccg ttg gga gat gtt att 1248 Lys Met Arg Glu Leu Gly Met Leu Thr Arg Pro Leu Gly Asp Val Ile 405 410 415 gca ttt ttg cct ccg tta gcg tct acc gcg gag gag ctg agt gag atg 1296 Ala Phe Leu Pro Pro Leu Ala Ser Thr Ala Glu Glu Leu Ser Glu Met 420 425 430 gta gca att atg aag caa gcc att cac gaa gtt act tcc ttg gaa gac 1344 Val Ala Ile Met Lys Gln Ala Ile His Glu Val Thr Ser Leu Glu Asp 435 440 445 tga 1347 <210> 97 <211> 448 <212> PRT <213> Bacillus subtilis <400> 97 Met Thr His Asp Leu Ile Glu Lys Ser Lys Lys His Leu Trp Leu Pro 1 5 10 15 Phe Thr Gln Met Lys Asp Tyr Asp Glu Asn Pro Leu Ile Ile Glu Ser 20 25 30 Gly Thr Gly Ile Lys Val Lys Asp Ile Asn Gly Lys Glu Tyr Tyr Asp 35 40 45 Gly Phe Ser Ser Val Trp Leu Asn Val His Gly His Arg Lys Lys Glu 50 55 60 Leu Asp Asp Ala Ile Lys Lys Gln Leu Gly Lys Ile Ala His Ser Thr 65 70 75 80 Leu Leu Gly Met Thr Asn Val Pro Ala Thr Gln Leu Ala Glu Thr Leu 85 90 95 Ile Asp Ile Ser Pro Lys Lys Leu Thr Arg Val Phe Tyr Ser Asp Ser 100 105 110 Gly Ala Glu Ala Met Glu Ile Ala Leu Lys Met Ala Phe Gln Tyr Trp 115 120 125 Lys Asn Ile Gly Lys Pro Glu Lys Gln Lys Phe Ile Ala Met Lys Asn 130 135 140 Gly Tyr His Gly Asp Thr Ile Gly Ala Val Ser Val Gly Ser Ile Glu 145 150 155 160 Leu Phe His His Val Tyr Gly Pro Leu Met Phe Glu Ser Tyr Lys Ala 165 170 175 Pro Ile Pro Tyr Val Tyr Arg Ser Glu Ser Gly Asp Pro Asp Glu Cys 180 185 190 Arg Asp Gln Cys Leu Arg Glu Leu Ala Gln Leu Leu Glu Glu His His 195 200 205 Glu Glu Ile Ala Ala Leu Ser Ile Glu Ser Met Val Gln Gly Ala Ser 210 215 220 Gly Met Ile Val Met Pro Glu Gly Tyr Leu Ala Gly Val Arg Glu Leu 225 230 235 240 Cys Thr Thr Tyr Asp Val Leu Met Ile Val Asp Glu Val Ala Thr Gly 245 250 255 Phe Gly Arg Thr Gly Lys Met Phe Ala Cys Glu His Glu Asn Val Gln 260 265 270 Pro Asp Leu Met Ala Ala Gly Lys Gly Ile Thr Gly Gly Tyr Leu Pro 275 280 285 Ile Ala Val Thr Phe Ala Thr Glu Asp Ile Tyr Lys Ala Phe Tyr Asp 290 295 300 Asp Tyr Glu Asn Leu Lys Thr Phe Phe His Gly His Ser Tyr Thr Gly 305 310 315 320 Asn Gln Leu Gly Cys Ala Val Ala Leu Glu Asn Leu Ala Leu Phe Glu 325 330 335 Ser Glu Asn Ile Val Glu Gln Val Ala Glu Lys Ser Lys Lys Leu His 340 345 350 Phe Leu Leu Gln Asp Leu His Ala Leu Pro His Val Gly Asp Ile Arg 355 360 365 Gln Leu Gly Phe Met Cys Gly Ala Glu Leu Val Arg Ser Lys Glu Thr 370 375 380 Lys Glu Pro Tyr Pro Ala Asp Arg Arg Ile Gly Tyr Lys Val Ser Leu 385 390 395 400 Lys Met Arg Glu Leu Gly Met Leu Thr Arg Pro Leu Gly Asp Val Ile 405 410 415 Ala Phe Leu Pro Pro Leu Ala Ser Thr Ala Glu Glu Leu Ser Glu Met 420 425 430 Val Ala Ile Met Lys Gln Ala Ile His Glu Val Thr Ser Leu Glu Asp 435 440 445 <210> 98 <211> 771 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(771) <223> bioH gene encoding Pimeloyl-[acyl-carrier protein] methyl ester esterase (BioH) <400> 98 atg aat aac atc tgg tgg cag acc aaa ggt cag ggg aat gtt cat ctt 48 Met Asn Asn Ile Trp Trp Gln Thr Lys Gly Gln Gly Asn Val His Leu 1 5 10 15 gtg ctg ctg cac gga tgg gga ctg aat gcc gaa gtg tgg cgt tgc att 96 Val Leu Leu His Gly Trp Gly Leu Asn Ala Glu Val Trp Arg Cys Ile 20 25 30 gac gag gaa ctt agc tcg cat ttt acg ctg cac ctt gtt gac ctg ccc 144 Asp Glu Glu Leu Ser Ser His Phe Thr Leu His Leu Val Asp Leu Pro 35 40 45 ggc ttc ggg cgt agc cgg gga ttt ggt gcg ctg tca ctt gct gat atg 192 Gly Phe Gly Arg Ser Arg Gly Phe Gly Ala Leu Ser Leu Ala Asp Met 50 55 60 gcc gaa gcc gtg ctg caa cag gca cct gat aaa gcc att tgg tta ggc 240 Ala Glu Ala Val Leu Gln Gln Ala Pro Asp Lys Ala Ile Trp Leu Gly 65 70 75 80 tgg agt ctg ggc ggg ctg gtg gca agc cag att gcg tta acc cat ccc 288 Trp Ser Leu Gly Gly Leu Val Ala Ser Gln Ile Ala Leu Thr His Pro 85 90 95 gag cgt gtt cag gcg ctg gtc acc gtg gcg tcg tca cct tgt ttt agt 336 Glu Arg Val Gln Ala Leu Val Thr Val Ala Ser Ser Pro Cys Phe Ser 100 105 110 gct cgt gac gag tgg ccg ggg ata aaa ccg gac gtg ctg gcg gga ttt 384 Ala Arg Asp Glu Trp Pro Gly Ile Lys Pro Asp Val Leu Ala Gly Phe 115 120 125 cag cag caa ctc agt gat gat ttt cag cgt aca gtg gag cgg ttc ctg 432 Gln Gln Gln Leu Ser Asp Asp Phe Gln Arg Thr Val Glu Arg Phe Leu 130 135 140 gcg tta caa acc atg ggg act gaa acg gcg cgc cag gat gcg cgg gcg 480 Ala Leu Gln Thr Met Gly Thr Glu Thr Ala Arg Gln Asp Ala Arg Ala 145 150 155 160 ttg aag aaa acc gtt ctg gcg tta ccg atg ccg gag gtt gac gtg ctt 528 Leu Lys Lys Thr Val Leu Ala Leu Pro Met Pro Glu Val Asp Val Leu 165 170 175 aat ggc ggg ctg gaa atc ctg aaa acg gtc gat ctc cgt cag ccg ctg 576 Asn Gly Gly Leu Glu Ile Leu Lys Thr Val Asp Leu Arg Gln Pro Leu 180 185 190 caa aac gtg tcc atg ccg ttt ttg cga ttg tat ggc tat ctc gac ggt 624 Gln Asn Val Ser Met Pro Phe Leu Arg Leu Tyr Gly Tyr Leu Asp Gly 195 200 205 ctg gtg ccg cgc aaa gtg gtg ccg atg ctg gat aaa ctt tgg cct cac 672 Leu Val Pro Arg Lys Val Val Pro Met Leu Asp Lys Leu Trp Pro His 210 215 220 agc gaa tca tat atc ttc gcc aaa gcg gcc cat gcg cca ttt att tcg 720 Ser Glu Ser Tyr Ile Phe Ala Lys Ala Ala His Ala Pro Phe Ile Ser 225 230 235 240 cat ccg gcc gag ttt tgt cac ctg ctg gtg gcg ttg aag cag agg gtg 768 His Pro Ala Glu Phe Cys His Leu Leu Val Ala Leu Lys Gln Arg Val 245 250 255 tag 771 <210> 99 <211> 256 <212> PRT <213> Escherichia coli <400> 99 Met Asn Asn Ile Trp Trp Gln Thr Lys Gly Gln Gly Asn Val His Leu 1 5 10 15 Val Leu Leu His Gly Trp Gly Leu Asn Ala Glu Val Trp Arg Cys Ile 20 25 30 Asp Glu Glu Leu Ser Ser His Phe Thr Leu His Leu Val Asp Leu Pro 35 40 45 Gly Phe Gly Arg Ser Arg Gly Phe Gly Ala Leu Ser Leu Ala Asp Met 50 55 60 Ala Glu Ala Val Leu Gln Gln Ala Pro Asp Lys Ala Ile Trp Leu Gly 65 70 75 80 Trp Ser Leu Gly Gly Leu Val Ala Ser Gln Ile Ala Leu Thr His Pro 85 90 95 Glu Arg Val Gln Ala Leu Val Thr Val Ala Ser Ser Pro Cys Phe Ser 100 105 110 Ala Arg Asp Glu Trp Pro Gly Ile Lys Pro Asp Val Leu Ala Gly Phe 115 120 125 Gln Gln Gln Leu Ser Asp Asp Phe Gln Arg Thr Val Glu Arg Phe Leu 130 135 140 Ala Leu Gln Thr Met Gly Thr Glu Thr Ala Arg Gln Asp Ala Arg Ala 145 150 155 160 Leu Lys Lys Thr Val Leu Ala Leu Pro Met Pro Glu Val Asp Val Leu 165 170 175 Asn Gly Gly Leu Glu Ile Leu Lys Thr Val Asp Leu Arg Gln Pro Leu 180 185 190 Gln Asn Val Ser Met Pro Phe Leu Arg Leu Tyr Gly Tyr Leu Asp Gly 195 200 205 Leu Val Pro Arg Lys Val Val Pro Met Leu Asp Lys Leu Trp Pro His 210 215 220 Ser Glu Ser Tyr Ile Phe Ala Lys Ala Ala His Ala Pro Phe Ile Ser 225 230 235 240 His Pro Ala Glu Phe Cys His Leu Leu Val Ala Leu Lys Gln Arg Val 245 250 255 <210> 100 <211> 777 <212> DNA <213> Bacillus subtilis <220> <221> CDS <222> (1)..(777) <223> bioW gene encoding 6-carboxyhexanoate-CoA ligase (BioW) <400> 100 atg caa gaa gag acg ttc tat tca gtg cgt atg cgc gct tca atg aat 48 Met Gln Glu Glu Thr Phe Tyr Ser Val Arg Met Arg Ala Ser Met Asn 1 5 10 15 ggc tcc cac gaa gat gga ggt aag cac atc tcc ggg ggt gag cgc ctt 96 Gly Ser His Glu Asp Gly Gly Lys His Ile Ser Gly Gly Glu Arg Leu 20 25 30 atc ccg ttc cac gag atg aaa cat acc gtc aac gct ttg ctt gag aag 144 Ile Pro Phe His Glu Met Lys His Thr Val Asn Ala Leu Leu Glu Lys 35 40 45 ggt ctt tct cat tct cgt ggg aaa cct gat ttt atg caa att cag ttt 192 Gly Leu Ser His Ser Arg Gly Lys Pro Asp Phe Met Gln Ile Gln Phe 50 55 60 gaa gag gtt cac gag tca atc aag aca atc cag ccc tta cct gtg cac 240 Glu Glu Val His Glu Ser Ile Lys Thr Ile Gln Pro Leu Pro Val His 65 70 75 80 acc aac gaa gtt agc tgc ccc gaa gaa gga caa aaa ctt gca cgc ttg 288 Thr Asn Glu Val Ser Cys Pro Glu Glu Gly Gln Lys Leu Ala Arg Leu 85 90 95 tta ctg gag aaa gaa ggg gtg agc cgc gac gtt att gaa aag gct tac 336 Leu Leu Glu Lys Glu Gly Val Ser Arg Asp Val Ile Glu Lys Ala Tyr 100 105 110 gaa caa att ccc gag tgg tcg gat gtc cgt ggt gcc gta ttg ttt gat 384 Glu Gln Ile Pro Glu Trp Ser Asp Val Arg Gly Ala Val Leu Phe Asp 115 120 125 att cat acg ggc aag cgt atg gat cag acg aaa gaa aag ggg gtg cgc 432 Ile His Thr Gly Lys Arg Met Asp Gln Thr Lys Glu Lys Gly Val Arg 130 135 140 gtc tct cgt atg gac tgg ccc gac gct aac ttt gag aaa tgg gcc tta 480 Val Ser Arg Met Asp Trp Pro Asp Ala Asn Phe Glu Lys Trp Ala Leu 145 150 155 160 cac agc cac gtg cca gca cat tca cgc atc aag gag gca ctg gca ctt 528 His Ser His Val Pro Ala His Ser Arg Ile Lys Glu Ala Leu Ala Leu 165 170 175 gct agc aag gtg tcc cgt cac ccg gca gtc gtt gcc gaa ttg tgt tgg 576 Ala Ser Lys Val Ser Arg His Pro Ala Val Val Ala Glu Leu Cys Trp 180 185 190 agc gac gat cca gat tac atc acc gga tat gta gct ggt aaa aaa atg 624 Ser Asp Asp Pro Asp Tyr Ile Thr Gly Tyr Val Ala Gly Lys Lys Met 195 200 205 ggg tac caa cgc att acc gca atg aag gag tac ggg acc gag gag gga 672 Gly Tyr Gln Arg Ile Thr Ala Met Lys Glu Tyr Gly Thr Glu Glu Gly 210 215 220 tgt cgt gtc ttc ttc atc gac ggc tcg aac gat gtt aat act tac att 720 Cys Arg Val Phe Phe Ile Asp Gly Ser Asn Asp Val Asn Thr Tyr Ile 225 230 235 240 cac gac ttg gag aaa cag ccg atc ctg att gaa tgg gaa gaa gac cac 768 His Asp Leu Glu Lys Gln Pro Ile Leu Ile Glu Trp Glu Glu Asp His 245 250 255 gat agc tga 777 Asp Ser <210> 101 <211> 258 <212> PRT <213> Bacillus subtilis <400> 101 Met Gln Glu Glu Thr Phe Tyr Ser Val Arg Met Arg Ala Ser Met Asn 1 5 10 15 Gly Ser His Glu Asp Gly Gly Lys His Ile Ser Gly Gly Glu Arg Leu 20 25 30 Ile Pro Phe His Glu Met Lys His Thr Val Asn Ala Leu Leu Glu Lys 35 40 45 Gly Leu Ser His Ser Arg Gly Lys Pro Asp Phe Met Gln Ile Gln Phe 50 55 60 Glu Glu Val His Glu Ser Ile Lys Thr Ile Gln Pro Leu Pro Val His 65 70 75 80 Thr Asn Glu Val Ser Cys Pro Glu Glu Gly Gln Lys Leu Ala Arg Leu 85 90 95 Leu Leu Glu Lys Glu Gly Val Ser Arg Asp Val Ile Glu Lys Ala Tyr 100 105 110 Glu Gln Ile Pro Glu Trp Ser Asp Val Arg Gly Ala Val Leu Phe Asp 115 120 125 Ile His Thr Gly Lys Arg Met Asp Gln Thr Lys Glu Lys Gly Val Arg 130 135 140 Val Ser Arg Met Asp Trp Pro Asp Ala Asn Phe Glu Lys Trp Ala Leu 145 150 155 160 His Ser His Val Pro Ala His Ser Arg Ile Lys Glu Ala Leu Ala Leu 165 170 175 Ala Ser Lys Val Ser Arg His Pro Ala Val Val Ala Glu Leu Cys Trp 180 185 190 Ser Asp Asp Pro Asp Tyr Ile Thr Gly Tyr Val Ala Gly Lys Lys Met 195 200 205 Gly Tyr Gln Arg Ile Thr Ala Met Lys Glu Tyr Gly Thr Glu Glu Gly 210 215 220 Cys Arg Val Phe Phe Ile Asp Gly Ser Asn Asp Val Asn Thr Tyr Ile 225 230 235 240 His Asp Leu Glu Lys Gln Pro Ile Leu Ile Glu Trp Glu Glu Asp His 245 250 255 Asp Ser <210> 102 <211> 966 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(966) <223> lipA gene encoding lipoic acid synthase (LipA) <400> 102 atg agt aaa ccc att gtg atg gaa cgc ggt gtt aaa tac cgc gat gcc 48 Met Ser Lys Pro Ile Val Met Glu Arg Gly Val Lys Tyr Arg Asp Ala 1 5 10 15 gat aag atg gcc ctt atc ccg gtt aaa aac gtg gca aca gag cgc gaa 96 Asp Lys Met Ala Leu Ile Pro Val Lys Asn Val Ala Thr Glu Arg Glu 20 25 30 gcc ctg ctg cgc aag ccg gaa tgg atg aaa atc aag ctt cca gcg gac 144 Ala Leu Leu Arg Lys Pro Glu Trp Met Lys Ile Lys Leu Pro Ala Asp 35 40 45 tct aca cgt atc cag ggc atc aaa gcc gca atg cgc aaa aat ggc ctg 192 Ser Thr Arg Ile Gln Gly Ile Lys Ala Ala Met Arg Lys Asn Gly Leu 50 55 60 cat tct gtc tgc gag gaa gcc tcc tgc cct aac ctg gcg gaa tgc ttc 240 His Ser Val Cys Glu Glu Ala Ser Cys Pro Asn Leu Ala Glu Cys Phe 65 70 75 80 aac cac ggc aca gca acg ttt atg atc ctc ggc gct att tgt acc cgc 288 Asn His Gly Thr Ala Thr Phe Met Ile Leu Gly Ala Ile Cys Thr Arg 85 90 95 cgt tgt ccg ttc tgt gat gtt gcc cac ggt cgc ccg gta gct cct gat 336 Arg Cys Pro Phe Cys Asp Val Ala His Gly Arg Pro Val Ala Pro Asp 100 105 110 gcc aat gaa cca gtg aaa ctg gcg cag acc att gcc gat atg gcg ctg 384 Ala Asn Glu Pro Val Lys Leu Ala Gln Thr Ile Ala Asp Met Ala Leu 115 120 125 cgt tat gtg gtt atc acc tcc gtt gac cgt gat gac ctg cgc gat ggc 432 Arg Tyr Val Val Ile Thr Ser Val Asp Arg Asp Asp Leu Arg Asp Gly 130 135 140 ggt gcc cag cac ttt gcg gat tgc att act gcc att cgg gaa aaa agc 480 Gly Ala Gln His Phe Ala Asp Cys Ile Thr Ala Ile Arg Glu Lys Ser 145 150 155 160 ccg caa atc aaa att gaa act ctg gtg ccg gat ttc cgc ggt cgt atg 528 Pro Gln Ile Lys Ile Glu Thr Leu Val Pro Asp Phe Arg Gly Arg Met 165 170 175 gat cgt gct ctg gat att ctg act gca acg cca cca gat gtg ttc aac 576 Asp Arg Ala Leu Asp Ile Leu Thr Ala Thr Pro Pro Asp Val Phe Asn 180 185 190 cat aac ctg gaa aac gta ccg cgt att tac cgt cag gta cgg cct ggt 624 His Asn Leu Glu Asn Val Pro Arg Ile Tyr Arg Gln Val Arg Pro Gly 195 200 205 gca gat tac aac tgg tcg ctg aag ctg ctg gaa cgc ttt aaa gaa gcg 672 Ala Asp Tyr Asn Trp Ser Leu Lys Leu Leu Glu Arg Phe Lys Glu Ala 210 215 220 cat ccg gaa atc ccg acc aag tct ggt ctg atg gtg gga ctg ggt gaa 720 His Pro Glu Ile Pro Thr Lys Ser Gly Leu Met Val Gly Leu Gly Glu 225 230 235 240 acc aat gaa gaa att att gag gta atg cgc gac ctg cgc cgt cat ggt 768 Thr Asn Glu Glu Ile Ile Glu Val Met Arg Asp Leu Arg Arg His Gly 245 250 255 gtg acg atg tta acg ctg ggg caa tat ttg cag cca agc cgc cat cac 816 Val Thr Met Leu Thr Leu Gly Gln Tyr Leu Gln Pro Ser Arg His His 260 265 270 ctg ccg gtt caa cgt tac gtt agc ccg gat gag ttc gac gaa atg aaa 864 Leu Pro Val Gln Arg Tyr Val Ser Pro Asp Glu Phe Asp Glu Met Lys 275 280 285 gcc gaa gcg ctg gcg atg ggc ttt acc cat gct gca tgc ggt ccg ttt 912 Ala Glu Ala Leu Ala Met Gly Phe Thr His Ala Ala Cys Gly Pro Phe 290 295 300 gtc cgc tct tct tac cac gcc gat ttg cag gcg aaa ggg atg gaa gtt 960 Val Arg Ser Ser Tyr His Ala Asp Leu Gln Ala Lys Gly Met Glu Val 305 310 315 320 aag taa 966 Lys <210> 103 <211> 321 <212> PRT <213> Escherichia coli <400> 103 Met Ser Lys Pro Ile Val Met Glu Arg Gly Val Lys Tyr Arg Asp Ala 1 5 10 15 Asp Lys Met Ala Leu Ile Pro Val Lys Asn Val Ala Thr Glu Arg Glu 20 25 30 Ala Leu Leu Arg Lys Pro Glu Trp Met Lys Ile Lys Leu Pro Ala Asp 35 40 45 Ser Thr Arg Ile Gln Gly Ile Lys Ala Ala Met Arg Lys Asn Gly Leu 50 55 60 His Ser Val Cys Glu Glu Ala Ser Cys Pro Asn Leu Ala Glu Cys Phe 65 70 75 80 Asn His Gly Thr Ala Thr Phe Met Ile Leu Gly Ala Ile Cys Thr Arg 85 90 95 Arg Cys Pro Phe Cys Asp Val Ala His Gly Arg Pro Val Ala Pro Asp 100 105 110 Ala Asn Glu Pro Val Lys Leu Ala Gln Thr Ile Ala Asp Met Ala Leu 115 120 125 Arg Tyr Val Val Ile Thr Ser Val Asp Arg Asp Asp Leu Arg Asp Gly 130 135 140 Gly Ala Gln His Phe Ala Asp Cys Ile Thr Ala Ile Arg Glu Lys Ser 145 150 155 160 Pro Gln Ile Lys Ile Glu Thr Leu Val Pro Asp Phe Arg Gly Arg Met 165 170 175 Asp Arg Ala Leu Asp Ile Leu Thr Ala Thr Pro Pro Asp Val Phe Asn 180 185 190 His Asn Leu Glu Asn Val Pro Arg Ile Tyr Arg Gln Val Arg Pro Gly 195 200 205 Ala Asp Tyr Asn Trp Ser Leu Lys Leu Leu Glu Arg Phe Lys Glu Ala 210 215 220 His Pro Glu Ile Pro Thr Lys Ser Gly Leu Met Val Gly Leu Gly Glu 225 230 235 240 Thr Asn Glu Glu Ile Ile Glu Val Met Arg Asp Leu Arg Arg His Gly 245 250 255 Val Thr Met Leu Thr Leu Gly Gln Tyr Leu Gln Pro Ser Arg His His 260 265 270 Leu Pro Val Gln Arg Tyr Val Ser Pro Asp Glu Phe Asp Glu Met Lys 275 280 285 Ala Glu Ala Leu Ala Met Gly Phe Thr His Ala Ala Cys Gly Pro Phe 290 295 300 Val Arg Ser Ser Tyr His Ala Asp Leu Gln Ala Lys Gly Met Glu Val 305 310 315 320 Lys <210> 104 <211> 897 <212> DNA <213> Bacillus subtilis subsp. Subtilis, str. 168 <220> <221> CDS <222> (1)..(897) <223> lipA gene encoding a lipoic acid synthase (LipA) <400> 104 atg gcg aag aag gat gaa cac ctg aga aag cca gaa tgg ctt aaa att 48 Met Ala Lys Lys Asp Glu His Leu Arg Lys Pro Glu Trp Leu Lys Ile 1 5 10 15 aag ctg aat aca aac gaa aac tac act ggc tta aaa aag tta atg cgt 96 Lys Leu Asn Thr Asn Glu Asn Tyr Thr Gly Leu Lys Lys Leu Met Arg 20 25 30 gag aat aac tta cat act gtc tgt gag gag gca aaa tgt cca aat ata 144 Glu Asn Asn Leu His Thr Val Cys Glu Glu Ala Lys Cys Pro Asn Ile 35 40 45 cac gaa tgc tgg gcc gtt cgg cgt acc gcg acg ttt atg ata ctg ggc 192 His Glu Cys Trp Ala Val Arg Arg Thr Ala Thr Phe Met Ile Leu Gly 50 55 60 tcc gtc tgc acg aga gca tgt cgt ttt tgc gcg gtt aaa acc ggc ctg 240 Ser Val Cys Thr Arg Ala Cys Arg Phe Cys Ala Val Lys Thr Gly Leu 65 70 75 80 ccg act gag ctt gac ttg caa gag cca gag cgc gtg gct gat tca gtt 288 Pro Thr Glu Leu Asp Leu Gln Glu Pro Glu Arg Val Ala Asp Ser Val 85 90 95 gcc ctt atg aac ctg aaa cac gcc gtt atc acg gcg gtc gcc cgt gac 336 Ala Leu Met Asn Leu Lys His Ala Val Ile Thr Ala Val Ala Arg Asp 100 105 110 gat caa aaa gat ggt gga gcg gga ata ttc gca gaa acg gta cgt gct 384 Asp Gln Lys Asp Gly Gly Ala Gly Ile Phe Ala Glu Thr Val Arg Ala 115 120 125 atc cgc cgg aag tct cca ttt acc acg att gaa gtg ctg ccg agc gat 432 Ile Arg Arg Lys Ser Pro Phe Thr Thr Ile Glu Val Leu Pro Ser Asp 130 135 140 atg ggc ggt aat tat gat aac ctt aag acc ttg atg gac aca cgg ccg 480 Met Gly Gly Asn Tyr Asp Asn Leu Lys Thr Leu Met Asp Thr Arg Pro 145 150 155 160 gat att ctg aat cac aac atc gag act gta cgg cgt tta aca cca aga 528 Asp Ile Leu Asn His Asn Ile Glu Thr Val Arg Arg Leu Thr Pro Arg 165 170 175 gtc aga gca cgc gct acc tat gat cgc agc ctt gaa ttc ttg cgt cgc 576 Val Arg Ala Arg Ala Thr Tyr Asp Arg Ser Leu Glu Phe Leu Arg Arg 180 185 190 gcc aaa gag atg cag ccc gac ata cca aca aaa agt agt ata atg atc 624 Ala Lys Glu Met Gln Pro Asp Ile Pro Thr Lys Ser Ser Ile Met Ile 195 200 205 ggc ttg gga gaa aca aaa gaa gaa atc atc gag gtc atg gat gac ctt 672 Gly Leu Gly Glu Thr Lys Glu Glu Ile Ile Glu Val Met Asp Asp Leu 210 215 220 ttg gca aac aac gtg gac ata atg gcc att ggg caa tac ttg caa cca 720 Leu Ala Asn Asn Val Asp Ile Met Ala Ile Gly Gln Tyr Leu Gln Pro 225 230 235 240 act aaa aag cac tta aaa gtt cag aaa tac tat cat cct gat gaa ttt 768 Thr Lys Lys His Leu Lys Val Gln Lys Tyr Tyr His Pro Asp Glu Phe 245 250 255 gcc gag ttg aag gaa atc gcc atg cag aag ggg ttt tca cat tgc gag 816 Ala Glu Leu Lys Glu Ile Ala Met Gln Lys Gly Phe Ser His Cys Glu 260 265 270 gcg ggt ccg ttg gtc cgt tca agt tac cac gcg gac gaa cag gtg aat 864 Ala Gly Pro Leu Val Arg Ser Ser Tyr His Ala Asp Glu Gln Val Asn 275 280 285 gaa gcg tca aag aaa cgc caa gca caa gct taa 897 Glu Ala Ser Lys Lys Arg Gln Ala Gln Ala 290 295 <210> 105 <211> 298 <212> PRT <213> Bacillus subtilis subsp. Subtilis, str. 168 <400> 105 Met Ala Lys Lys Asp Glu His Leu Arg Lys Pro Glu Trp Leu Lys Ile 1 5 10 15 Lys Leu Asn Thr Asn Glu Asn Tyr Thr Gly Leu Lys Lys Leu Met Arg 20 25 30 Glu Asn Asn Leu His Thr Val Cys Glu Glu Ala Lys Cys Pro Asn Ile 35 40 45 His Glu Cys Trp Ala Val Arg Arg Thr Ala Thr Phe Met Ile Leu Gly 50 55 60 Ser Val Cys Thr Arg Ala Cys Arg Phe Cys Ala Val Lys Thr Gly Leu 65 70 75 80 Pro Thr Glu Leu Asp Leu Gln Glu Pro Glu Arg Val Ala Asp Ser Val 85 90 95 Ala Leu Met Asn Leu Lys His Ala Val Ile Thr Ala Val Ala Arg Asp 100 105 110 Asp Gln Lys Asp Gly Gly Ala Gly Ile Phe Ala Glu Thr Val Arg Ala 115 120 125 Ile Arg Arg Lys Ser Pro Phe Thr Thr Ile Glu Val Leu Pro Ser Asp 130 135 140 Met Gly Gly Asn Tyr Asp Asn Leu Lys Thr Leu Met Asp Thr Arg Pro 145 150 155 160 Asp Ile Leu Asn His Asn Ile Glu Thr Val Arg Arg Leu Thr Pro Arg 165 170 175 Val Arg Ala Arg Ala Thr Tyr Asp Arg Ser Leu Glu Phe Leu Arg Arg 180 185 190 Ala Lys Glu Met Gln Pro Asp Ile Pro Thr Lys Ser Ser Ile Met Ile 195 200 205 Gly Leu Gly Glu Thr Lys Glu Glu Ile Ile Glu Val Met Asp Asp Leu 210 215 220 Leu Ala Asn Asn Val Asp Ile Met Ala Ile Gly Gln Tyr Leu Gln Pro 225 230 235 240 Thr Lys Lys His Leu Lys Val Gln Lys Tyr Tyr His Pro Asp Glu Phe 245 250 255 Ala Glu Leu Lys Glu Ile Ala Met Gln Lys Gly Phe Ser His Cys Glu 260 265 270 Ala Gly Pro Leu Val Arg Ser Ser Tyr His Ala Asp Glu Gln Val Asn 275 280 285 Glu Ala Ser Lys Lys Arg Gln Ala Gln Ala 290 295 <210> 106 <211> 1245 <212> DNA <213> Saccharomyces cerevisiae, S288C <220> <221> CDS <222> (1)..(1245) <223> lipA gene encoding a lipoic acid synthase (LipA) <400> 106 atg tac aga cgt agt gta ggg gtg ctt ttc gta ggg cgt aac act cgg 48 Met Tyr Arg Arg Ser Val Gly Val Leu Phe Val Gly Arg Asn Thr Arg 1 5 10 15 tgg atc agc agc acg atc cgg tgt ggc act agc gca acc cgc cct att 96 Trp Ile Ser Ser Thr Ile Arg Cys Gly Thr Ser Ala Thr Arg Pro Ile 20 25 30 cgt agt aac gcg ttg aac act gac tca gat aac gct agt gtg cgc gta 144 Arg Ser Asn Ala Leu Asn Thr Asp Ser Asp Asn Ala Ser Val Arg Val 35 40 45 ccc gta ggg aac agc acg gag gta gaa aat gcg acc tcg caa ctg aca 192 Pro Val Gly Asn Ser Thr Glu Val Glu Asn Ala Thr Ser Gln Leu Thr 50 55 60 ggt act tcg ggc aag aga cgg aag gga aat aga aag cgg ata acg gaa 240 Gly Thr Ser Gly Lys Arg Arg Lys Gly Asn Arg Lys Arg Ile Thr Glu 65 70 75 80 ttt aaa gat gcc tta aac ctg ggt ccc tcg ttc gcc gat ttc gta tca 288 Phe Lys Asp Ala Leu Asn Leu Gly Pro Ser Phe Ala Asp Phe Val Ser 85 90 95 ggt aag gct tcg aaa atg att ttg gat cct ttg gag aaa gcc cgc cag 336 Gly Lys Ala Ser Lys Met Ile Leu Asp Pro Leu Glu Lys Ala Arg Gln 100 105 110 aac aca gag gag gct aag aag tta cct aga tgg ttg aaa gtg cct att 384 Asn Thr Glu Glu Ala Lys Lys Leu Pro Arg Trp Leu Lys Val Pro Ile 115 120 125 cct aaa ggc acg aac tac cac aaa ttg aaa ggt gat gta aaa gaa tta 432 Pro Lys Gly Thr Asn Tyr His Lys Leu Lys Gly Asp Val Lys Glu Leu 130 135 140 gga ctt tcc act gtc tgt gag gaa gct cgc tgc cca aat atc gga gag 480 Gly Leu Ser Thr Val Cys Glu Glu Ala Arg Cys Pro Asn Ile Gly Glu 145 150 155 160 tgc tgg gga gga aag gac aaa tcc aag gct acg gcg acg ata atg ttg 528 Cys Trp Gly Gly Lys Asp Lys Ser Lys Ala Thr Ala Thr Ile Met Leu 165 170 175 ctt ggc gat acg tgc acg cgc ggt tgc cgt ttt tgc tct gta aag act 576 Leu Gly Asp Thr Cys Thr Arg Gly Cys Arg Phe Cys Ser Val Lys Thr 180 185 190 aac cgc aca ccc tcg aaa cct gat cct atg gag ccc gaa aat aca gca 624 Asn Arg Thr Pro Ser Lys Pro Asp Pro Met Glu Pro Glu Asn Thr Ala 195 200 205 gag gcg att aaa cgt tgg ggc ctg ggc tat gtt gta tta act aca gta 672 Glu Ala Ile Lys Arg Trp Gly Leu Gly Tyr Val Val Leu Thr Thr Val 210 215 220 gat cgt gat gat tta gtc gac ggt ggg gca aat cat ttg gcg gag act 720 Asp Arg Asp Asp Leu Val Asp Gly Gly Ala Asn His Leu Ala Glu Thr 225 230 235 240 gtc aga aag atc aag caa aaa gcg ccg aat act ctt gtg gag act ctt 768 Val Arg Lys Ile Lys Gln Lys Ala Pro Asn Thr Leu Val Glu Thr Leu 245 250 255 tcc ggt gac ttt aga ggg gac tta aag atg gtt gac atc atg gca caa 816 Ser Gly Asp Phe Arg Gly Asp Leu Lys Met Val Asp Ile Met Ala Gln 260 265 270 tgc ggc tta gat gtt tac gct cat aac tta gaa acc gtt gaa agt tta 864 Cys Gly Leu Asp Val Tyr Ala His Asn Leu Glu Thr Val Glu Ser Leu 275 280 285 act ccc cac gta aga gac cgg cgg gcc aca tac aga caa agt ctg agc 912 Thr Pro His Val Arg Asp Arg Arg Ala Thr Tyr Arg Gln Ser Leu Ser 290 295 300 gtt ttg gag cgt gcc aaa gcc acg gtg cca tca tta atc act aaa acc 960 Val Leu Glu Arg Ala Lys Ala Thr Val Pro Ser Leu Ile Thr Lys Thr 305 310 315 320 tct ata atg tta ggc tta ggg gaa acg gac gaa caa ata acg caa acc 1008 Ser Ile Met Leu Gly Leu Gly Glu Thr Asp Glu Gln Ile Thr Gln Thr 325 330 335 tta aaa gat tta cgg aac att caa tgc gac gtc gtg acc ttt ggt caa 1056 Leu Lys Asp Leu Arg Asn Ile Gln Cys Asp Val Val Thr Phe Gly Gln 340 345 350 tat atg cgg cca aca aag cgg cac atg aag gtg gtc gag tac gta aaa 1104 Tyr Met Arg Pro Thr Lys Arg His Met Lys Val Val Glu Tyr Val Lys 355 360 365 ccc gaa aaa ttt gat tac tgg aag gag cgg gct ctt gag atg ggt ttc 1152 Pro Glu Lys Phe Asp Tyr Trp Lys Glu Arg Ala Leu Glu Met Gly Phe 370 375 380 tta tat tgc gcc tct ggg cca ctt gta cgc tct agc tat aaa gcg ggt 1200 Leu Tyr Cys Ala Ser Gly Pro Leu Val Arg Ser Ser Tyr Lys Ala Gly 385 390 395 400 gag gca ttt atc gag aat gtt tta aaa aaa aga aac atg aag tga 1245 Glu Ala Phe Ile Glu Asn Val Leu Lys Lys Arg Asn Met Lys 405 410 <210> 107 <211> 414 <212> PRT <213> Saccharomyces cerevisiae, S288C <400> 107 Met Tyr Arg Arg Ser Val Gly Val Leu Phe Val Gly Arg Asn Thr Arg 1 5 10 15 Trp Ile Ser Ser Thr Ile Arg Cys Gly Thr Ser Ala Thr Arg Pro Ile 20 25 30 Arg Ser Asn Ala Leu Asn Thr Asp Ser Asp Asn Ala Ser Val Arg Val 35 40 45 Pro Val Gly Asn Ser Thr Glu Val Glu Asn Ala Thr Ser Gln Leu Thr 50 55 60 Gly Thr Ser Gly Lys Arg Arg Lys Gly Asn Arg Lys Arg Ile Thr Glu 65 70 75 80 Phe Lys Asp Ala Leu Asn Leu Gly Pro Ser Phe Ala Asp Phe Val Ser 85 90 95 Gly Lys Ala Ser Lys Met Ile Leu Asp Pro Leu Glu Lys Ala Arg Gln 100 105 110 Asn Thr Glu Glu Ala Lys Lys Leu Pro Arg Trp Leu Lys Val Pro Ile 115 120 125 Pro Lys Gly Thr Asn Tyr His Lys Leu Lys Gly Asp Val Lys Glu Leu 130 135 140 Gly Leu Ser Thr Val Cys Glu Glu Ala Arg Cys Pro Asn Ile Gly Glu 145 150 155 160 Cys Trp Gly Gly Lys Asp Lys Ser Lys Ala Thr Ala Thr Ile Met Leu 165 170 175 Leu Gly Asp Thr Cys Thr Arg Gly Cys Arg Phe Cys Ser Val Lys Thr 180 185 190 Asn Arg Thr Pro Ser Lys Pro Asp Pro Met Glu Pro Glu Asn Thr Ala 195 200 205 Glu Ala Ile Lys Arg Trp Gly Leu Gly Tyr Val Val Leu Thr Thr Val 210 215 220 Asp Arg Asp Asp Leu Val Asp Gly Gly Ala Asn His Leu Ala Glu Thr 225 230 235 240 Val Arg Lys Ile Lys Gln Lys Ala Pro Asn Thr Leu Val Glu Thr Leu 245 250 255 Ser Gly Asp Phe Arg Gly Asp Leu Lys Met Val Asp Ile Met Ala Gln 260 265 270 Cys Gly Leu Asp Val Tyr Ala His Asn Leu Glu Thr Val Glu Ser Leu 275 280 285 Thr Pro His Val Arg Asp Arg Arg Ala Thr Tyr Arg Gln Ser Leu Ser 290 295 300 Val Leu Glu Arg Ala Lys Ala Thr Val Pro Ser Leu Ile Thr Lys Thr 305 310 315 320 Ser Ile Met Leu Gly Leu Gly Glu Thr Asp Glu Gln Ile Thr Gln Thr 325 330 335 Leu Lys Asp Leu Arg Asn Ile Gln Cys Asp Val Val Thr Phe Gly Gln 340 345 350 Tyr Met Arg Pro Thr Lys Arg His Met Lys Val Val Glu Tyr Val Lys 355 360 365 Pro Glu Lys Phe Asp Tyr Trp Lys Glu Arg Ala Leu Glu Met Gly Phe 370 375 380 Leu Tyr Cys Ala Ser Gly Pro Leu Val Arg Ser Ser Tyr Lys Ala Gly 385 390 395 400 Glu Ala Phe Ile Glu Asn Val Leu Lys Lys Arg Asn Met Lys 405 410 <210> 108 <211> 1017 <212> DNA <213> Pseudomonas putida KT2440 <220> <221> CDS <222> (1)..(1017) <223> lipA gene encoding a lipoic acid synthase (LipA) <400> 108 atg acg acc gtt cag gaa gcc gtc ccg aat ttg atc ccc acg caa gat 48 Met Thr Thr Val Gln Glu Ala Val Pro Asn Leu Ile Pro Thr Gln Asp 1 5 10 15 gct acg ccg cgc ccg gct ccc aaa aag gtt gaa gct ggt gtg aag ttg 96 Ala Thr Pro Arg Pro Ala Pro Lys Lys Val Glu Ala Gly Val Lys Leu 20 25 30 cgc gga gcg gat aaa gtt gcc cgc atc cct gtg aaa att att ccg aca 144 Arg Gly Ala Asp Lys Val Ala Arg Ile Pro Val Lys Ile Ile Pro Thr 35 40 45 gat gag tta ccg aaa aaa cct gac tgg atc cgc gtc cgc atc cct gta 192 Asp Glu Leu Pro Lys Lys Pro Asp Trp Ile Arg Val Arg Ile Pro Val 50 55 60 tcg ccc gag gtt gat cgt atc aaa caa ttg ttg cgt aag cac aaa ttg 240 Ser Pro Glu Val Asp Arg Ile Lys Gln Leu Leu Arg Lys His Lys Leu 65 70 75 80 cat agc gtc tgc gag gag gcc tcc tgc cca aat ttg ggc gag tgc ttt 288 His Ser Val Cys Glu Glu Ala Ser Cys Pro Asn Leu Gly Glu Cys Phe 85 90 95 agc ggc ggc acg gct act ttc atg att atg ggc gac atc tgt aca cgt 336 Ser Gly Gly Thr Ala Thr Phe Met Ile Met Gly Asp Ile Cys Thr Arg 100 105 110 cgt tgt cct ttt tgc gac gtg gga cac gga cgc cca aag ccg ctg gat 384 Arg Cys Pro Phe Cys Asp Val Gly His Gly Arg Pro Lys Pro Leu Asp 115 120 125 ttg gat gag cca aaa aat ctt gca gtt gcg att gca gac ttg cgc tta 432 Leu Asp Glu Pro Lys Asn Leu Ala Val Ala Ile Ala Asp Leu Arg Leu 130 135 140 aag tac gtg gtt atc aca tcg gtt gat cgt gac gat tta cgt gac ggg 480 Lys Tyr Val Val Ile Thr Ser Val Asp Arg Asp Asp Leu Arg Asp Gly 145 150 155 160 ggc gct caa cat ttt gcc gac tgc atc cgt gag atc cgc gca ctg tcc 528 Gly Ala Gln His Phe Ala Asp Cys Ile Arg Glu Ile Arg Ala Leu Ser 165 170 175 ccg ggg gtg cag ctg gag act ttg gtt ccg gac tac cgt gga cgc atg 576 Pro Gly Val Gln Leu Glu Thr Leu Val Pro Asp Tyr Arg Gly Arg Met 180 185 190 gat gtt gca ttg gag att acc gcc cag gag cct cca gat gtg ttc aac 624 Asp Val Ala Leu Glu Ile Thr Ala Gln Glu Pro Pro Asp Val Phe Asn 195 200 205 cat aat ctt gag aca gtc cca cgc tta tat aag gct gct cgt ccg ggt 672 His Asn Leu Glu Thr Val Pro Arg Leu Tyr Lys Ala Ala Arg Pro Gly 210 215 220 tct gat tac gac tgg agt tta gac ttg ttg caa aaa ttt aag cag ctg 720 Ser Asp Tyr Asp Trp Ser Leu Asp Leu Leu Gln Lys Phe Lys Gln Leu 225 230 235 240 gtt ccg cat gtg cca act aag tct gga ctg atg tta gga tta gga gaa 768 Val Pro His Val Pro Thr Lys Ser Gly Leu Met Leu Gly Leu Gly Glu 245 250 255 aca gat gag gaa gtc att gaa gta atg cat cgt atg cgt gag cat gat 816 Thr Asp Glu Glu Val Ile Glu Val Met His Arg Met Arg Glu His Asp 260 265 270 atc gac atg ttg act ctg gga cag tac ctt cag ccc tcg cgt tcg cat 864 Ile Asp Met Leu Thr Leu Gly Gln Tyr Leu Gln Pro Ser Arg Ser His 275 280 285 ctt cct gtt cag cgt ttt gtt cat ccc gat act ttt gct tgg ttc gcg 912 Leu Pro Val Gln Arg Phe Val His Pro Asp Thr Phe Ala Trp Phe Ala 290 295 300 gaa gag gga tac aag atg ggg ttc aaa aat gtc gct tct gga cca ttg 960 Glu Glu Gly Tyr Lys Met Gly Phe Lys Asn Val Ala Ser Gly Pro Leu 305 310 315 320 gta cgc tcg tca tat cac gca gac cag cag gct cat gag gcc aaa att 1008 Val Arg Ser Ser Tyr His Ala Asp Gln Gln Ala His Glu Ala Lys Ile 325 330 335 aag ctt tga 1017 Lys Leu <210> 109 <211> 338 <212> PRT <213> Pseudomonas putida KT2440 <400> 109 Met Thr Thr Val Gln Glu Ala Val Pro Asn Leu Ile Pro Thr Gln Asp 1 5 10 15 Ala Thr Pro Arg Pro Ala Pro Lys Lys Val Glu Ala Gly Val Lys Leu 20 25 30 Arg Gly Ala Asp Lys Val Ala Arg Ile Pro Val Lys Ile Ile Pro Thr 35 40 45 Asp Glu Leu Pro Lys Lys Pro Asp Trp Ile Arg Val Arg Ile Pro Val 50 55 60 Ser Pro Glu Val Asp Arg Ile Lys Gln Leu Leu Arg Lys His Lys Leu 65 70 75 80 His Ser Val Cys Glu Glu Ala Ser Cys Pro Asn Leu Gly Glu Cys Phe 85 90 95 Ser Gly Gly Thr Ala Thr Phe Met Ile Met Gly Asp Ile Cys Thr Arg 100 105 110 Arg Cys Pro Phe Cys Asp Val Gly His Gly Arg Pro Lys Pro Leu Asp 115 120 125 Leu Asp Glu Pro Lys Asn Leu Ala Val Ala Ile Ala Asp Leu Arg Leu 130 135 140 Lys Tyr Val Val Ile Thr Ser Val Asp Arg Asp Asp Leu Arg Asp Gly 145 150 155 160 Gly Ala Gln His Phe Ala Asp Cys Ile Arg Glu Ile Arg Ala Leu Ser 165 170 175 Pro Gly Val Gln Leu Glu Thr Leu Val Pro Asp Tyr Arg Gly Arg Met 180 185 190 Asp Val Ala Leu Glu Ile Thr Ala Gln Glu Pro Pro Asp Val Phe Asn 195 200 205 His Asn Leu Glu Thr Val Pro Arg Leu Tyr Lys Ala Ala Arg Pro Gly 210 215 220 Ser Asp Tyr Asp Trp Ser Leu Asp Leu Leu Gln Lys Phe Lys Gln Leu 225 230 235 240 Val Pro His Val Pro Thr Lys Ser Gly Leu Met Leu Gly Leu Gly Glu 245 250 255 Thr Asp Glu Glu Val Ile Glu Val Met His Arg Met Arg Glu His Asp 260 265 270 Ile Asp Met Leu Thr Leu Gly Gln Tyr Leu Gln Pro Ser Arg Ser His 275 280 285 Leu Pro Val Gln Arg Phe Val His Pro Asp Thr Phe Ala Trp Phe Ala 290 295 300 Glu Glu Gly Tyr Lys Met Gly Phe Lys Asn Val Ala Ser Gly Pro Leu 305 310 315 320 Val Arg Ser Ser Tyr His Ala Asp Gln Gln Ala His Glu Ala Lys Ile 325 330 335 Lys Leu <210> 110 <211> 867 <212> DNA <213> Bacteroides fragilis 638R <220> <221> CDS <222> (1)..(867) <223> lipA gene encoding a lipoic acid synthase (LipA) <400> 110 atg ggg aac gac aag cgc gtt cgc aag cct gag tgg tta aaa att tct 48 Met Gly Asn Asp Lys Arg Val Arg Lys Pro Glu Trp Leu Lys Ile Ser 1 5 10 15 att ggt gca aat gag cgc tac acc gag act aaa cgt atc gtg gaa agc 96 Ile Gly Ala Asn Glu Arg Tyr Thr Glu Thr Lys Arg Ile Val Glu Ser 20 25 30 cat tgt ctt cac acc atc tgc agt tct ggg cgc tgc ccg aat atg ggg 144 His Cys Leu His Thr Ile Cys Ser Ser Gly Arg Cys Pro Asn Met Gly 35 40 45 gaa tgt tgg ggg aaa ggg aca gca acc ttt atg atc gct ggt gac atc 192 Glu Cys Trp Gly Lys Gly Thr Ala Thr Phe Met Ile Ala Gly Asp Ile 50 55 60 tgc act cgc tct tgc aag ttc tgt aat acc caa acc ggg cgc ccc tta 240 Cys Thr Arg Ser Cys Lys Phe Cys Asn Thr Gln Thr Gly Arg Pro Leu 65 70 75 80 cct tta gac ccg gat gaa ccc acc cac gtt gcc gaa tct att gca tta 288 Pro Leu Asp Pro Asp Glu Pro Thr His Val Ala Glu Ser Ile Ala Leu 85 90 95 atg aag ctg tca cat gca gtc att aca agc gta gac cgt gac gac ctt 336 Met Lys Leu Ser His Ala Val Ile Thr Ser Val Asp Arg Asp Asp Leu 100 105 110 ccg gac tta gga gca gca cat tgg gct cag act atc cgc gag atc aag 384 Pro Asp Leu Gly Ala Ala His Trp Ala Gln Thr Ile Arg Glu Ile Lys 115 120 125 cgt ttg aat ccg gaa act acc aca gag gtt tta att cct gac ttt cag 432 Arg Leu Asn Pro Glu Thr Thr Thr Glu Val Leu Ile Pro Asp Phe Gln 130 135 140 gga cgt aag gaa ctt atc gac caa gtc att aag gcg tgt ccc gaa att 480 Gly Arg Lys Glu Leu Ile Asp Gln Val Ile Lys Ala Cys Pro Glu Ile 145 150 155 160 att tca cat aac atg gaa acg gtc aaa cgc att tcg ccg cag gtt cgt 528 Ile Ser His Asn Met Glu Thr Val Lys Arg Ile Ser Pro Gln Val Arg 165 170 175 tct gca gcg aat tac cac act agt ctt gaa gtc att cgt cag att gct 576 Ser Ala Ala Asn Tyr His Thr Ser Leu Glu Val Ile Arg Gln Ile Ala 180 185 190 gaa agc ggg atc act gct aaa tcg ggc att atg gtt ggg ttg ggt gag 624 Glu Ser Gly Ile Thr Ala Lys Ser Gly Ile Met Val Gly Leu Gly Glu 195 200 205 act ccc gcc gaa gtc gaa gag ctt atg gac gac ttg atc tca gtc ggt 672 Thr Pro Ala Glu Val Glu Glu Leu Met Asp Asp Leu Ile Ser Val Gly 210 215 220 tgc aaa atc ctg acc atc ggt caa tat ctt caa cct aca cat aag cat 720 Cys Lys Ile Leu Thr Ile Gly Gln Tyr Leu Gln Pro Thr His Lys His 225 230 235 240 ttc ccg gtt gct gct tac att acc cca gaa cag ttc gcc gtc tat aag 768 Phe Pro Val Ala Ala Tyr Ile Thr Pro Glu Gln Phe Ala Val Tyr Lys 245 250 255 gag acg ggc ttg aag aaa ggt ttt gag cag gtg gag tca gcg ccc ctt 816 Glu Thr Gly Leu Lys Lys Gly Phe Glu Gln Val Glu Ser Ala Pro Leu 260 265 270 gtg cgc tct tct tat cac gca gaa aaa cac atc cgc ttt aat aac aag 864 Val Arg Ser Ser Tyr His Ala Glu Lys His Ile Arg Phe Asn Asn Lys 275 280 285 taa 867 <210> 111 <211> 288 <212> PRT <213> Bacteroides fragilis 638R <400> 111 Met Gly Asn Asp Lys Arg Val Arg Lys Pro Glu Trp Leu Lys Ile Ser 1 5 10 15 Ile Gly Ala Asn Glu Arg Tyr Thr Glu Thr Lys Arg Ile Val Glu Ser 20 25 30 His Cys Leu His Thr Ile Cys Ser Ser Gly Arg Cys Pro Asn Met Gly 35 40 45 Glu Cys Trp Gly Lys Gly Thr Ala Thr Phe Met Ile Ala Gly Asp Ile 50 55 60 Cys Thr Arg Ser Cys Lys Phe Cys Asn Thr Gln Thr Gly Arg Pro Leu 65 70 75 80 Pro Leu Asp Pro Asp Glu Pro Thr His Val Ala Glu Ser Ile Ala Leu 85 90 95 Met Lys Leu Ser His Ala Val Ile Thr Ser Val Asp Arg Asp Asp Leu 100 105 110 Pro Asp Leu Gly Ala Ala His Trp Ala Gln Thr Ile Arg Glu Ile Lys 115 120 125 Arg Leu Asn Pro Glu Thr Thr Thr Glu Val Leu Ile Pro Asp Phe Gln 130 135 140 Gly Arg Lys Glu Leu Ile Asp Gln Val Ile Lys Ala Cys Pro Glu Ile 145 150 155 160 Ile Ser His Asn Met Glu Thr Val Lys Arg Ile Ser Pro Gln Val Arg 165 170 175 Ser Ala Ala Asn Tyr His Thr Ser Leu Glu Val Ile Arg Gln Ile Ala 180 185 190 Glu Ser Gly Ile Thr Ala Lys Ser Gly Ile Met Val Gly Leu Gly Glu 195 200 205 Thr Pro Ala Glu Val Glu Glu Leu Met Asp Asp Leu Ile Ser Val Gly 210 215 220 Cys Lys Ile Leu Thr Ile Gly Gln Tyr Leu Gln Pro Thr His Lys His 225 230 235 240 Phe Pro Val Ala Ala Tyr Ile Thr Pro Glu Gln Phe Ala Val Tyr Lys 245 250 255 Glu Thr Gly Leu Lys Lys Gly Phe Glu Gln Val Glu Ser Ala Pro Leu 260 265 270 Val Arg Ser Ser Tyr His Ala Glu Lys His Ile Arg Phe Asn Asn Lys 275 280 285 <210> 112 <211> 954 <212> DNA <213> Streptomyces coelicolor A3(2) <220> <221> CDS <222> (1)..(954) <223> lipA gene encoding a lipoic acid synthase (LipA) <400> 112 atg agc gcg gta gct cct gac ggc cgc aag atg ctg cgt ttg gag gtt 48 Met Ser Ala Val Ala Pro Asp Gly Arg Lys Met Leu Arg Leu Glu Val 1 5 10 15 cgt aac agc caa acg cct atc gaa cgc aag ccc gag tgg atc aag act 96 Arg Asn Ser Gln Thr Pro Ile Glu Arg Lys Pro Glu Trp Ile Lys Thr 20 25 30 cgt gct aag atg ggt ccc gaa tac acg aag atg cag aac ttg gtc aag 144 Arg Ala Lys Met Gly Pro Glu Tyr Thr Lys Met Gln Asn Leu Val Lys 35 40 45 tcg gag ggc tta cat acg gta tgc cag gaa gct ggg tgc ccc aac att 192 Ser Glu Gly Leu His Thr Val Cys Gln Glu Ala Gly Cys Pro Asn Ile 50 55 60 tat gag tgc tgg gag gat cgt gaa gca acg ttt ttg atc gga ggc gat 240 Tyr Glu Cys Trp Glu Asp Arg Glu Ala Thr Phe Leu Ile Gly Gly Asp 65 70 75 80 cag tgc act cgt cgc tgc gac ttt tgt caa atc gat aca gga aaa cct 288 Gln Cys Thr Arg Arg Cys Asp Phe Cys Gln Ile Asp Thr Gly Lys Pro 85 90 95 gaa gcc ctt gat cgt gat gag cca cgt cgt gta gga gaa tcg gtc gtc 336 Glu Ala Leu Asp Arg Asp Glu Pro Arg Arg Val Gly Glu Ser Val Val 100 105 110 aca atg gat ctt aac tat gct acc att act ggc gtt gca cgt gat gat 384 Thr Met Asp Leu Asn Tyr Ala Thr Ile Thr Gly Val Ala Arg Asp Asp 115 120 125 ctg ccc gat ggt gga gct tgg ctt tac gct gaa act gtt cgt cag atc 432 Leu Pro Asp Gly Gly Ala Trp Leu Tyr Ala Glu Thr Val Arg Gln Ile 130 135 140 cat gaa cag act gcg ggt cgc gaa gcc ggg cgt acc aaa gtt gaa ctt 480 His Glu Gln Thr Ala Gly Arg Glu Ala Gly Arg Thr Lys Val Glu Leu 145 150 155 160 ctt gca cct gat ttt aac gcg gtg cca gag ctg tta cgc gaa gtc ttt 528 Leu Ala Pro Asp Phe Asn Ala Val Pro Glu Leu Leu Arg Glu Val Phe 165 170 175 gaa tcg cgc cct gaa gtc ttc gct cac aat gta gag aca gta cca cgc 576 Glu Ser Arg Pro Glu Val Phe Ala His Asn Val Glu Thr Val Pro Arg 180 185 190 atc ttt aag cgt att cgt cca ggg ttt cgc tat gag cgc agt ctt aag 624 Ile Phe Lys Arg Ile Arg Pro Gly Phe Arg Tyr Glu Arg Ser Leu Lys 195 200 205 gtt atc act gat gcg cgt gat ttt gga ttg gtc acc aaa tcg aac ctt 672 Val Ile Thr Asp Ala Arg Asp Phe Gly Leu Val Thr Lys Ser Asn Leu 210 215 220 atc ttg gga atg ggg gaa aca cgc gag gaa att tcg gaa gcc tta aaa 720 Ile Leu Gly Met Gly Glu Thr Arg Glu Glu Ile Ser Glu Ala Leu Lys 225 230 235 240 cag ctg cat gaa gcg ggt tgc gag ctt att aca atc acc cag tat ctt 768 Gln Leu His Glu Ala Gly Cys Glu Leu Ile Thr Ile Thr Gln Tyr Leu 245 250 255 cgt cct agt gtc cgt cat cat ccg gtc gaa cgc tgg gta aaa ccc cag 816 Arg Pro Ser Val Arg His His Pro Val Glu Arg Trp Val Lys Pro Gln 260 265 270 gaa ttt gtg gaa ctt aag gag gag gcg gag caa att ggc ttt tcg ggt 864 Glu Phe Val Glu Leu Lys Glu Glu Ala Glu Gln Ile Gly Phe Ser Gly 275 280 285 gta atg tca ggc ccc ctt gtg cgt tcc tcg tac cgt gcg ggg cgc ttg 912 Val Met Ser Gly Pro Leu Val Arg Ser Ser Tyr Arg Ala Gly Arg Leu 290 295 300 tac gga atg gct atg gaa cag cgc cgt tcc gca acc gtt tga 954 Tyr Gly Met Ala Met Glu Gln Arg Arg Ser Ala Thr Val 305 310 315 <210> 113 <211> 317 <212> PRT <213> Streptomyces coelicolor A3(2) <400> 113 Met Ser Ala Val Ala Pro Asp Gly Arg Lys Met Leu Arg Leu Glu Val 1 5 10 15 Arg Asn Ser Gln Thr Pro Ile Glu Arg Lys Pro Glu Trp Ile Lys Thr 20 25 30 Arg Ala Lys Met Gly Pro Glu Tyr Thr Lys Met Gln Asn Leu Val Lys 35 40 45 Ser Glu Gly Leu His Thr Val Cys Gln Glu Ala Gly Cys Pro Asn Ile 50 55 60 Tyr Glu Cys Trp Glu Asp Arg Glu Ala Thr Phe Leu Ile Gly Gly Asp 65 70 75 80 Gln Cys Thr Arg Arg Cys Asp Phe Cys Gln Ile Asp Thr Gly Lys Pro 85 90 95 Glu Ala Leu Asp Arg Asp Glu Pro Arg Arg Val Gly Glu Ser Val Val 100 105 110 Thr Met Asp Leu Asn Tyr Ala Thr Ile Thr Gly Val Ala Arg Asp Asp 115 120 125 Leu Pro Asp Gly Gly Ala Trp Leu Tyr Ala Glu Thr Val Arg Gln Ile 130 135 140 His Glu Gln Thr Ala Gly Arg Glu Ala Gly Arg Thr Lys Val Glu Leu 145 150 155 160 Leu Ala Pro Asp Phe Asn Ala Val Pro Glu Leu Leu Arg Glu Val Phe 165 170 175 Glu Ser Arg Pro Glu Val Phe Ala His Asn Val Glu Thr Val Pro Arg 180 185 190 Ile Phe Lys Arg Ile Arg Pro Gly Phe Arg Tyr Glu Arg Ser Leu Lys 195 200 205 Val Ile Thr Asp Ala Arg Asp Phe Gly Leu Val Thr Lys Ser Asn Leu 210 215 220 Ile Leu Gly Met Gly Glu Thr Arg Glu Glu Ile Ser Glu Ala Leu Lys 225 230 235 240 Gln Leu His Glu Ala Gly Cys Glu Leu Ile Thr Ile Thr Gln Tyr Leu 245 250 255 Arg Pro Ser Val Arg His His Pro Val Glu Arg Trp Val Lys Pro Gln 260 265 270 Glu Phe Val Glu Leu Lys Glu Glu Ala Glu Gln Ile Gly Phe Ser Gly 275 280 285 Val Met Ser Gly Pro Leu Val Arg Ser Ser Tyr Arg Ala Gly Arg Leu 290 295 300 Tyr Gly Met Ala Met Glu Gln Arg Arg Ser Ala Thr Val 305 310 315 <210> 114 <211> 642 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(642) <223> lipB encoding octanoyltransferase (LipB) <400> 114 atg tat cag gat aaa att ctt gtc cgc cag ctc ggt ctt cag cct tac 48 Met Tyr Gln Asp Lys Ile Leu Val Arg Gln Leu Gly Leu Gln Pro Tyr 1 5 10 15 gag cca atc tcc cag gct atg cat gaa ttc acc gat acc cgc gat gat 96 Glu Pro Ile Ser Gln Ala Met His Glu Phe Thr Asp Thr Arg Asp Asp 20 25 30 agt acc ctt gat gaa atc tgg ctg gtc gag cac tat ccg gta ttc acc 144 Ser Thr Leu Asp Glu Ile Trp Leu Val Glu His Tyr Pro Val Phe Thr 35 40 45 caa ggt cag gca gga aaa gcg gag cac att tta atg ccg ggt gat att 192 Gln Gly Gln Ala Gly Lys Ala Glu His Ile Leu Met Pro Gly Asp Ile 50 55 60 ccg gtg atc cag agc gat cgc ggt ggg cag gtg act tat cac ggg ccg 240 Pro Val Ile Gln Ser Asp Arg Gly Gly Gln Val Thr Tyr His Gly Pro 65 70 75 80 ggg caa cag gtg atg tat gtg ttg ctt aac ctg aaa cgc cgt aaa ctc 288 Gly Gln Gln Val Met Tyr Val Leu Leu Asn Leu Lys Arg Arg Lys Leu 85 90 95 ggt gtg cgt gaa ctg gtg acc ttg ctt gag caa aca gtg gtg aat acc 336 Gly Val Arg Glu Leu Val Thr Leu Leu Glu Gln Thr Val Val Asn Thr 100 105 110 ctg gct gaa ctg ggt ata gaa gcg cat cct cgg gct gac gcg cca ggt 384 Leu Ala Glu Leu Gly Ile Glu Ala His Pro Arg Ala Asp Ala Pro Gly 115 120 125 gtc tat gtt ggg gaa aag aaa att tgc tca ctg ggt tta cgt att cga 432 Val Tyr Val Gly Glu Lys Lys Ile Cys Ser Leu Gly Leu Arg Ile Arg 130 135 140 cgc ggt tgt tca ttc cac ggt ctg gca tta aac gtc aat atg gat ctt 480 Arg Gly Cys Ser Phe His Gly Leu Ala Leu Asn Val Asn Met Asp Leu 145 150 155 160 tca cca ttt tta cgt att aat cct tgt ggg tat gcc gga atg gaa atg 528 Ser Pro Phe Leu Arg Ile Asn Pro Cys Gly Tyr Ala Gly Met Glu Met 165 170 175 gct aaa ata tca caa tgg aaa ccc gaa gcg acg act aat aat att gct 576 Ala Lys Ile Ser Gln Trp Lys Pro Glu Ala Thr Thr Asn Asn Ile Ala 180 185 190 cca cgt tta ctg gaa aat att tta gcg cta cta aac aat ccg gac ttc 624 Pro Arg Leu Leu Glu Asn Ile Leu Ala Leu Leu Asn Asn Pro Asp Phe 195 200 205 gaa tat att acc gct taa 642 Glu Tyr Ile Thr Ala 210 <210> 115 <211> 213 <212> PRT <213> Escherichia coli <400> 115 Met Tyr Gln Asp Lys Ile Leu Val Arg Gln Leu Gly Leu Gln Pro Tyr 1 5 10 15 Glu Pro Ile Ser Gln Ala Met His Glu Phe Thr Asp Thr Arg Asp Asp 20 25 30 Ser Thr Leu Asp Glu Ile Trp Leu Val Glu His Tyr Pro Val Phe Thr 35 40 45 Gln Gly Gln Ala Gly Lys Ala Glu His Ile Leu Met Pro Gly Asp Ile 50 55 60 Pro Val Ile Gln Ser Asp Arg Gly Gly Gln Val Thr Tyr His Gly Pro 65 70 75 80 Gly Gln Gln Val Met Tyr Val Leu Leu Asn Leu Lys Arg Arg Lys Leu 85 90 95 Gly Val Arg Glu Leu Val Thr Leu Leu Glu Gln Thr Val Val Asn Thr 100 105 110 Leu Ala Glu Leu Gly Ile Glu Ala His Pro Arg Ala Asp Ala Pro Gly 115 120 125 Val Tyr Val Gly Glu Lys Lys Ile Cys Ser Leu Gly Leu Arg Ile Arg 130 135 140 Arg Gly Cys Ser Phe His Gly Leu Ala Leu Asn Val Asn Met Asp Leu 145 150 155 160 Ser Pro Phe Leu Arg Ile Asn Pro Cys Gly Tyr Ala Gly Met Glu Met 165 170 175 Ala Lys Ile Ser Gln Trp Lys Pro Glu Ala Thr Thr Asn Asn Ile Ala 180 185 190 Pro Arg Leu Leu Glu Asn Ile Leu Ala Leu Leu Asn Asn Pro Asp Phe 195 200 205 Glu Tyr Ile Thr Ala 210 <210> 116 <211> 576 <212> DNA <213> Shigella flexneri <220> <221> CDS <222> (1)..(576) <223> lipB gene encoding a octanoyltransferase (LipB) <400> 116 atg cat gaa ttc acc gat acc cgc gat aat agt acc ctt gat gaa atc 48 Met His Glu Phe Thr Asp Thr Arg Asp Asn Ser Thr Leu Asp Glu Ile 1 5 10 15 tgg ctg gtc gag cac tat ccg gta ttc acc caa ggt cag gca gga aaa 96 Trp Leu Val Glu His Tyr Pro Val Phe Thr Gln Gly Gln Ala Gly Lys 20 25 30 gcg gag cac att tta atg ccg ggt gat att ccg gtg atc cag agc gat 144 Ala Glu His Ile Leu Met Pro Gly Asp Ile Pro Val Ile Gln Ser Asp 35 40 45 cgc ggt ggg cag gtg act tat cac ggg ccg gga caa cag gtg atg tat 192 Arg Gly Gly Gln Val Thr Tyr His Gly Pro Gly Gln Gln Val Met Tyr 50 55 60 gtg ttg ctt aac ctg aaa cgc cgt aaa ctc ggt gtg cgt gaa ctg gtg 240 Val Leu Leu Asn Leu Lys Arg Arg Lys Leu Gly Val Arg Glu Leu Val 65 70 75 80 acc ttg ctt gag caa aca gtg gtg aat acc ctg gct gaa ctg ggt ata 288 Thr Leu Leu Glu Gln Thr Val Val Asn Thr Leu Ala Glu Leu Gly Ile 85 90 95 gaa gcg cat cct cgg gct gac gcg cct ggt gtc tat gtc ggg gaa aag 336 Glu Ala His Pro Arg Ala Asp Ala Pro Gly Val Tyr Val Gly Glu Lys 100 105 110 aaa att tgc tca ctg ggt tta cga att cga cgc ggt tgt tca ttc cac 384 Lys Ile Cys Ser Leu Gly Leu Arg Ile Arg Arg Gly Cys Ser Phe His 115 120 125 ggt ctg gca tta aac gtc aat atg gat ctt tca cca ttt tta cgt att 432 Gly Leu Ala Leu Asn Val Asn Met Asp Leu Ser Pro Phe Leu Arg Ile 130 135 140 aat cct tgt ggg tat gcc gga atg gaa atg gct aaa ata tca caa tgg 480 Asn Pro Cys Gly Tyr Ala Gly Met Glu Met Ala Lys Ile Ser Gln Trp 145 150 155 160 aaa ccc gaa gcg acg act aat aat att gct cca cgt tta ctg gaa aat 528 Lys Pro Glu Ala Thr Thr Asn Asn Ile Ala Pro Arg Leu Leu Glu Asn 165 170 175 att tta gcg cta cta aac aat ccg gac ttc gaa tat att acc gct taa 576 Ile Leu Ala Leu Leu Asn Asn Pro Asp Phe Glu Tyr Ile Thr Ala 180 185 190 <210> 117 <211> 191 <212> PRT <213> Shigella flexneri <400> 117 Met His Glu Phe Thr Asp Thr Arg Asp Asn Ser Thr Leu Asp Glu Ile 1 5 10 15 Trp Leu Val Glu His Tyr Pro Val Phe Thr Gln Gly Gln Ala Gly Lys 20 25 30 Ala Glu His Ile Leu Met Pro Gly Asp Ile Pro Val Ile Gln Ser Asp 35 40 45 Arg Gly Gly Gln Val Thr Tyr His Gly Pro Gly Gln Gln Val Met Tyr 50 55 60 Val Leu Leu Asn Leu Lys Arg Arg Lys Leu Gly Val Arg Glu Leu Val 65 70 75 80 Thr Leu Leu Glu Gln Thr Val Val Asn Thr Leu Ala Glu Leu Gly Ile 85 90 95 Glu Ala His Pro Arg Ala Asp Ala Pro Gly Val Tyr Val Gly Glu Lys 100 105 110 Lys Ile Cys Ser Leu Gly Leu Arg Ile Arg Arg Gly Cys Ser Phe His 115 120 125 Gly Leu Ala Leu Asn Val Asn Met Asp Leu Ser Pro Phe Leu Arg Ile 130 135 140 Asn Pro Cys Gly Tyr Ala Gly Met Glu Met Ala Lys Ile Ser Gln Trp 145 150 155 160 Lys Pro Glu Ala Thr Thr Asn Asn Ile Ala Pro Arg Leu Leu Glu Asn 165 170 175 Ile Leu Ala Leu Leu Asn Asn Pro Asp Phe Glu Tyr Ile Thr Ala 180 185 190 <210> 118 <211> 1893 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(1893) <223> aceF gene encoding dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase (E2) <400> 118 atg gct atc gaa atc aaa gta ccg gac atc ggg gct gat gaa gtt gaa 48 Met Ala Ile Glu Ile Lys Val Pro Asp Ile Gly Ala Asp Glu Val Glu 1 5 10 15 atc acc gag atc ctg gtc aaa gtg ggc gac aaa gtt gaa gcc gaa cag 96 Ile Thr Glu Ile Leu Val Lys Val Gly Asp Lys Val Glu Ala Glu Gln 20 25 30 tcg ctg atc acc gta gaa ggc gac aaa gcc tct atg gaa gtt ccg tct 144 Ser Leu Ile Thr Val Glu Gly Asp Lys Ala Ser Met Glu Val Pro Ser 35 40 45 ccg cag gcg ggt atc gtt aaa gag atc aaa gtc tct gtt ggc gat aaa 192 Pro Gln Ala Gly Ile Val Lys Glu Ile Lys Val Ser Val Gly Asp Lys 50 55 60 acc cag acc ggc gca ctg att atg att ttc gat tcc gcc gac ggt gca 240 Thr Gln Thr Gly Ala Leu Ile Met Ile Phe Asp Ser Ala Asp Gly Ala 65 70 75 80 gca gac gct gca cct gct cag gca gaa gag aag aaa gaa gca gct ccg 288 Ala Asp Ala Ala Pro Ala Gln Ala Glu Glu Lys Lys Glu Ala Ala Pro 85 90 95 gca gca gca cca gcg gct gcg gcg gca aaa gac gtt aac gtt ccg gat 336 Ala Ala Ala Pro Ala Ala Ala Ala Ala Lys Asp Val Asn Val Pro Asp 100 105 110 atc ggc agc gac gaa gtt gaa gtg acc gaa atc ctg gtg aaa gtt ggc 384 Ile Gly Ser Asp Glu Val Glu Val Thr Glu Ile Leu Val Lys Val Gly 115 120 125 gat aaa gtt gaa gct gaa cag tcg ctg atc acc gta gaa ggc gac aag 432 Asp Lys Val Glu Ala Glu Gln Ser Leu Ile Thr Val Glu Gly Asp Lys 130 135 140 gct tct atg gaa gtt ccg gct ccg ttt gct ggc acc gtg aaa gag atc 480 Ala Ser Met Glu Val Pro Ala Pro Phe Ala Gly Thr Val Lys Glu Ile 145 150 155 160 aaa gtg aac gtg ggt gac aaa gtg tct acc ggc tcg ctg att atg gtc 528 Lys Val Asn Val Gly Asp Lys Val Ser Thr Gly Ser Leu Ile Met Val 165 170 175 ttc gaa gtc gcg ggt gaa gca ggc gcg gca gct ccg gcc gct aaa cag 576 Phe Glu Val Ala Gly Glu Ala Gly Ala Ala Ala Pro Ala Ala Lys Gln 180 185 190 gaa gca gct ccg gca gcg gcc cct gca cca gcg gct ggc gtg aaa gaa 624 Glu Ala Ala Pro Ala Ala Ala Pro Ala Pro Ala Ala Gly Val Lys Glu 195 200 205 gtt aac gtt ccg gat atc ggc ggt gac gaa gtt gaa gtg act gaa gtg 672 Val Asn Val Pro Asp Ile Gly Gly Asp Glu Val Glu Val Thr Glu Val 210 215 220 atg gtg aaa gtg ggc gac aaa gtt gcc gct gaa cag tca ctg atc acc 720 Met Val Lys Val Gly Asp Lys Val Ala Ala Glu Gln Ser Leu Ile Thr 225 230 235 240 gta gaa ggc gac aaa gct tct atg gaa gtt ccg gcg ccg ttt gca ggc 768 Val Glu Gly Asp Lys Ala Ser Met Glu Val Pro Ala Pro Phe Ala Gly 245 250 255 gtc gtg aag gaa ctg aaa gtc aac gtt ggc gat aaa gtg aaa act ggc 816 Val Val Lys Glu Leu Lys Val Asn Val Gly Asp Lys Val Lys Thr Gly 260 265 270 tcg ctg att atg atc ttc gaa gtt gaa ggc gca gcg cct gcg gca gct 864 Ser Leu Ile Met Ile Phe Glu Val Glu Gly Ala Ala Pro Ala Ala Ala 275 280 285 cct gcg aaa cag gaa gcg gca gcg ccg gca ccg gca gca aaa gct gaa 912 Pro Ala Lys Gln Glu Ala Ala Ala Pro Ala Pro Ala Ala Lys Ala Glu 290 295 300 gcc ccg gca gca gca cca gct gcg aaa gcg gaa ggc aaa tct gaa ttt 960 Ala Pro Ala Ala Ala Pro Ala Ala Lys Ala Glu Gly Lys Ser Glu Phe 305 310 315 320 gct gaa aac gac gct tat gtt cac gcg act ccg ctg atc cgc cgt ctg 1008 Ala Glu Asn Asp Ala Tyr Val His Ala Thr Pro Leu Ile Arg Arg Leu 325 330 335 gca cgc gag ttt ggt gtt aac ctt gcg aaa gtg aag ggc act ggc cgt 1056 Ala Arg Glu Phe Gly Val Asn Leu Ala Lys Val Lys Gly Thr Gly Arg 340 345 350 aaa ggt cgt atc ctg cgc gaa gac gtt cag gct tac gtg aaa gaa gct 1104 Lys Gly Arg Ile Leu Arg Glu Asp Val Gln Ala Tyr Val Lys Glu Ala 355 360 365 atc aaa cgt gca gaa gca gct ccg gca gcg act ggc ggt ggt atc cct 1152 Ile Lys Arg Ala Glu Ala Ala Pro Ala Ala Thr Gly Gly Gly Ile Pro 370 375 380 ggc atg ctg ccg tgg ccg aag gtg gac ttc agc aag ttt ggt gaa atc 1200 Gly Met Leu Pro Trp Pro Lys Val Asp Phe Ser Lys Phe Gly Glu Ile 385 390 395 400 gaa gaa gtg gaa ctg ggc cgc atc cag aaa atc tct ggt gcg aac ctg 1248 Glu Glu Val Glu Leu Gly Arg Ile Gln Lys Ile Ser Gly Ala Asn Leu 405 410 415 agc cgt aac tgg gta atg atc ccg cat gtt act cac ttc gac aaa acc 1296 Ser Arg Asn Trp Val Met Ile Pro His Val Thr His Phe Asp Lys Thr 420 425 430 gat atc acc gag ttg gaa gcg ttc cgt aaa cag cag aac gaa gaa gcg 1344 Asp Ile Thr Glu Leu Glu Ala Phe Arg Lys Gln Gln Asn Glu Glu Ala 435 440 445 gcg aaa cgt aag ctg gat gtg aag atc acc ccg gtt gtc ttc atc atg 1392 Ala Lys Arg Lys Leu Asp Val Lys Ile Thr Pro Val Val Phe Ile Met 450 455 460 aaa gcc gtt gct gca gct ctt gag cag atg cct cgc ttc aat agt tcg 1440 Lys Ala Val Ala Ala Ala Leu Glu Gln Met Pro Arg Phe Asn Ser Ser 465 470 475 480 ctg tcg gaa gac ggt cag cgt ctg acc ctg aag aaa tac atc aac atc 1488 Leu Ser Glu Asp Gly Gln Arg Leu Thr Leu Lys Lys Tyr Ile Asn Ile 485 490 495 ggt gtg gcg gtg gat acc ccg aac ggt ctg gtt gtt ccg gta ttc aaa 1536 Gly Val Ala Val Asp Thr Pro Asn Gly Leu Val Val Pro Val Phe Lys 500 505 510 gac gtc aac aag aaa ggc atc atc gag ctg tct cgc gag ctg atg act 1584 Asp Val Asn Lys Lys Gly Ile Ile Glu Leu Ser Arg Glu Leu Met Thr 515 520 525 att tct aag aaa gcg cgt gac ggt aag ctg act gcg ggc gaa atg cag 1632 Ile Ser Lys Lys Ala Arg Asp Gly Lys Leu Thr Ala Gly Glu Met Gln 530 535 540 ggc ggt tgc ttc acc atc tcc agc atc ggc ggc ctg ggt act acc cac 1680 Gly Gly Cys Phe Thr Ile Ser Ser Ile Gly Gly Leu Gly Thr Thr His 545 550 555 560 ttc gcg ccg att gtg aac gcg ccg gaa gtg gct atc ctc ggc gtt tcc 1728 Phe Ala Pro Ile Val Asn Ala Pro Glu Val Ala Ile Leu Gly Val Ser 565 570 575 aag tcc gcg atg gag ccg gtg tgg aat ggt aaa gag ttc gtg ccg cgt 1776 Lys Ser Ala Met Glu Pro Val Trp Asn Gly Lys Glu Phe Val Pro Arg 580 585 590 ctg atg ctg ccg att tct ctc tcc ttc gac cac cgc gtg atc gac ggt 1824 Leu Met Leu Pro Ile Ser Leu Ser Phe Asp His Arg Val Ile Asp Gly 595 600 605 gct gat ggt gcc cgt ttc att acc atc att aac aac acg ctg tct gac 1872 Ala Asp Gly Ala Arg Phe Ile Thr Ile Ile Asn Asn Thr Leu Ser Asp 610 615 620 att cgc cgt ctg gtg atg taa 1893 Ile Arg Arg Leu Val Met 625 630 <210> 119 <211> 630 <212> PRT <213> Escherichia coli <400> 119 Met Ala Ile Glu Ile Lys Val Pro Asp Ile Gly Ala Asp Glu Val Glu 1 5 10 15 Ile Thr Glu Ile Leu Val Lys Val Gly Asp Lys Val Glu Ala Glu Gln 20 25 30 Ser Leu Ile Thr Val Glu Gly Asp Lys Ala Ser Met Glu Val Pro Ser 35 40 45 Pro Gln Ala Gly Ile Val Lys Glu Ile Lys Val Ser Val Gly Asp Lys 50 55 60 Thr Gln Thr Gly Ala Leu Ile Met Ile Phe Asp Ser Ala Asp Gly Ala 65 70 75 80 Ala Asp Ala Ala Pro Ala Gln Ala Glu Glu Lys Lys Glu Ala Ala Pro 85 90 95 Ala Ala Ala Pro Ala Ala Ala Ala Ala Lys Asp Val Asn Val Pro Asp 100 105 110 Ile Gly Ser Asp Glu Val Glu Val Thr Glu Ile Leu Val Lys Val Gly 115 120 125 Asp Lys Val Glu Ala Glu Gln Ser Leu Ile Thr Val Glu Gly Asp Lys 130 135 140 Ala Ser Met Glu Val Pro Ala Pro Phe Ala Gly Thr Val Lys Glu Ile 145 150 155 160 Lys Val Asn Val Gly Asp Lys Val Ser Thr Gly Ser Leu Ile Met Val 165 170 175 Phe Glu Val Ala Gly Glu Ala Gly Ala Ala Ala Pro Ala Ala Lys Gln 180 185 190 Glu Ala Ala Pro Ala Ala Ala Pro Ala Pro Ala Ala Gly Val Lys Glu 195 200 205 Val Asn Val Pro Asp Ile Gly Gly Asp Glu Val Glu Val Thr Glu Val 210 215 220 Met Val Lys Val Gly Asp Lys Val Ala Ala Glu Gln Ser Leu Ile Thr 225 230 235 240 Val Glu Gly Asp Lys Ala Ser Met Glu Val Pro Ala Pro Phe Ala Gly 245 250 255 Val Val Lys Glu Leu Lys Val Asn Val Gly Asp Lys Val Lys Thr Gly 260 265 270 Ser Leu Ile Met Ile Phe Glu Val Glu Gly Ala Ala Pro Ala Ala Ala 275 280 285 Pro Ala Lys Gln Glu Ala Ala Ala Pro Ala Pro Ala Ala Lys Ala Glu 290 295 300 Ala Pro Ala Ala Ala Pro Ala Ala Lys Ala Glu Gly Lys Ser Glu Phe 305 310 315 320 Ala Glu Asn Asp Ala Tyr Val His Ala Thr Pro Leu Ile Arg Arg Leu 325 330 335 Ala Arg Glu Phe Gly Val Asn Leu Ala Lys Val Lys Gly Thr Gly Arg 340 345 350 Lys Gly Arg Ile Leu Arg Glu Asp Val Gln Ala Tyr Val Lys Glu Ala 355 360 365 Ile Lys Arg Ala Glu Ala Ala Pro Ala Ala Thr Gly Gly Gly Ile Pro 370 375 380 Gly Met Leu Pro Trp Pro Lys Val Asp Phe Ser Lys Phe Gly Glu Ile 385 390 395 400 Glu Glu Val Glu Leu Gly Arg Ile Gln Lys Ile Ser Gly Ala Asn Leu 405 410 415 Ser Arg Asn Trp Val Met Ile Pro His Val Thr His Phe Asp Lys Thr 420 425 430 Asp Ile Thr Glu Leu Glu Ala Phe Arg Lys Gln Gln Asn Glu Glu Ala 435 440 445 Ala Lys Arg Lys Leu Asp Val Lys Ile Thr Pro Val Val Phe Ile Met 450 455 460 Lys Ala Val Ala Ala Ala Leu Glu Gln Met Pro Arg Phe Asn Ser Ser 465 470 475 480 Leu Ser Glu Asp Gly Gln Arg Leu Thr Leu Lys Lys Tyr Ile Asn Ile 485 490 495 Gly Val Ala Val Asp Thr Pro Asn Gly Leu Val Val Pro Val Phe Lys 500 505 510 Asp Val Asn Lys Lys Gly Ile Ile Glu Leu Ser Arg Glu Leu Met Thr 515 520 525 Ile Ser Lys Lys Ala Arg Asp Gly Lys Leu Thr Ala Gly Glu Met Gln 530 535 540 Gly Gly Cys Phe Thr Ile Ser Ser Ile Gly Gly Leu Gly Thr Thr His 545 550 555 560 Phe Ala Pro Ile Val Asn Ala Pro Glu Val Ala Ile Leu Gly Val Ser 565 570 575 Lys Ser Ala Met Glu Pro Val Trp Asn Gly Lys Glu Phe Val Pro Arg 580 585 590 Leu Met Leu Pro Ile Ser Leu Ser Phe Asp His Arg Val Ile Asp Gly 595 600 605 Ala Asp Gly Ala Arg Phe Ile Thr Ile Ile Asn Asn Thr Leu Ser Asp 610 615 620 Ile Arg Arg Leu Val Met 625 630 <210> 120 <211> 1890 <212> DNA <213> Klebsiella oxytoca <220> <221> CDS <222> (1)..(1890) <223> aceF gene encoding dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase (E2) <400> 120 atg gct atc gag atc aag gtg ccc gac atc ggc gct gac gaa gta gaa 48 Met Ala Ile Glu Ile Lys Val Pro Asp Ile Gly Ala Asp Glu Val Glu 1 5 10 15 att acc gag atc ctg gtt aaa gtc ggt gat aag gta gag gca gaa cag 96 Ile Thr Glu Ile Leu Val Lys Val Gly Asp Lys Val Glu Ala Glu Gln 20 25 30 agt tta atc act gta gaa ggc gat aag gca tcc atg gag gta cca tca 144 Ser Leu Ile Thr Val Glu Gly Asp Lys Ala Ser Met Glu Val Pro Ser 35 40 45 ccg caa gct ggt gta gtt aaa gag atc aaa gtg agt gta ggc gac aaa 192 Pro Gln Ala Gly Val Val Lys Glu Ile Lys Val Ser Val Gly Asp Lys 50 55 60 act gaa acc ggt aag tta att atg atc ttc gat tca gcc gac ggg gca 240 Thr Glu Thr Gly Lys Leu Ile Met Ile Phe Asp Ser Ala Asp Gly Ala 65 70 75 80 gcc gcc gct gct ccc gca cag gaa gag aaa aag gag gcg gca cct gcc 288 Ala Ala Ala Ala Pro Ala Gln Glu Glu Lys Lys Glu Ala Ala Pro Ala 85 90 95 gct gca gca cca gca gcc gct tct gca aaa gag gtg cat gta cct gac 336 Ala Ala Ala Pro Ala Ala Ala Ser Ala Lys Glu Val His Val Pro Asp 100 105 110 att gga ggc gac gaa gta gaa gta aca gag att atg gtc aag gtt ggc 384 Ile Gly Gly Asp Glu Val Glu Val Thr Glu Ile Met Val Lys Val Gly 115 120 125 gat aca atc gca gcg gaa caa agc tta att acg gta gaa ggc gat aaa 432 Asp Thr Ile Ala Ala Glu Gln Ser Leu Ile Thr Val Glu Gly Asp Lys 130 135 140 gca agc atg gaa gtt ccc gct ccc ttc gct ggg act gta aaa gaa atc 480 Ala Ser Met Glu Val Pro Ala Pro Phe Ala Gly Thr Val Lys Glu Ile 145 150 155 160 aag att aac acc ggc gac aag gtt tcc acc ggc tca tta att atg atc 528 Lys Ile Asn Thr Gly Asp Lys Val Ser Thr Gly Ser Leu Ile Met Ile 165 170 175 ttc gaa gta gca gga gct gca cct gcg gca gcg cct gcg aaa gcg gag 576 Phe Glu Val Ala Gly Ala Ala Pro Ala Ala Ala Pro Ala Lys Ala Glu 180 185 190 gct gca cct gca gcg gcg gct ccc gcc gct agt ggt agt aaa gaa gtg 624 Ala Ala Pro Ala Ala Ala Ala Pro Ala Ala Ser Gly Ser Lys Glu Val 195 200 205 cac gtt ccg gac atc gga ggt gac gag gtc gaa gtc act gaa gtg atg 672 His Val Pro Asp Ile Gly Gly Asp Glu Val Glu Val Thr Glu Val Met 210 215 220 gta aaa gca ggg gat aaa atc gca gcc gag cag agt tta att aca gtc 720 Val Lys Ala Gly Asp Lys Ile Ala Ala Glu Gln Ser Leu Ile Thr Val 225 230 235 240 gaa ggc gat aag gcg tct atg gaa gtt cca gcg cca ttc gcc ggt aca 768 Glu Gly Asp Lys Ala Ser Met Glu Val Pro Ala Pro Phe Ala Gly Thr 245 250 255 gta aag gaa att aag atc agc act gga gat aaa gtc tca act ggt tca 816 Val Lys Glu Ile Lys Ile Ser Thr Gly Asp Lys Val Ser Thr Gly Ser 260 265 270 ttg atc atg gtc ttc gaa gtc gaa ggc gcc gca cct gcg gcg gca ccg 864 Leu Ile Met Val Phe Glu Val Glu Gly Ala Ala Pro Ala Ala Ala Pro 275 280 285 gca gcg gct gcc gct cca gca cca gct gct gcg ccc gca caa gct gca 912 Ala Ala Ala Ala Ala Pro Ala Pro Ala Ala Ala Pro Ala Gln Ala Ala 290 295 300 aaa cca gct gcc ccc gca gcg aag gcc gaa ggc aag agc gag ttc gca 960 Lys Pro Ala Ala Pro Ala Ala Lys Ala Glu Gly Lys Ser Glu Phe Ala 305 310 315 320 gag aat gat gcg tac gta cat gcg aca cca ttg att cgc cgt ttg gca 1008 Glu Asn Asp Ala Tyr Val His Ala Thr Pro Leu Ile Arg Arg Leu Ala 325 330 335 cgc gaa ttc ggt gtg aac ctg gct aaa gtg aag gga acg ggg cgc aaa 1056 Arg Glu Phe Gly Val Asn Leu Ala Lys Val Lys Gly Thr Gly Arg Lys 340 345 350 ggc cgc att ttg cgt gag gac gtc cag gct tat gtt aaa gaa gca gtc 1104 Gly Arg Ile Leu Arg Glu Asp Val Gln Ala Tyr Val Lys Glu Ala Val 355 360 365 aag cgt gcc gaa gca gcg cca gcc gca acc ggc ggg ggg atc cca ggc 1152 Lys Arg Ala Glu Ala Ala Pro Ala Ala Thr Gly Gly Gly Ile Pro Gly 370 375 380 atg tta ccc tgg cca aag gta gac ttt tca aaa ttt ggg gag gta gaa 1200 Met Leu Pro Trp Pro Lys Val Asp Phe Ser Lys Phe Gly Glu Val Glu 385 390 395 400 gag gtt gag ttg gga cgc atc cag aag atc tcc ggt gca aat ttg tcg 1248 Glu Val Glu Leu Gly Arg Ile Gln Lys Ile Ser Gly Ala Asn Leu Ser 405 410 415 cgc aac tgg gtc atg att ccg cac gtc act cac ttt gac aaa acg gac 1296 Arg Asn Trp Val Met Ile Pro His Val Thr His Phe Asp Lys Thr Asp 420 425 430 att acc gat ttg gag gct ttt cgt aag caa caa aat gct gag gcg gag 1344 Ile Thr Asp Leu Glu Ala Phe Arg Lys Gln Gln Asn Ala Glu Ala Glu 435 440 445 aag cgt aaa ttg gac gtg aag ttc acc ccg gtg gtg ttc att atg aag 1392 Lys Arg Lys Leu Asp Val Lys Phe Thr Pro Val Val Phe Ile Met Lys 450 455 460 gca gtg gcc gca gca ctt gaa cag atg ccg cgt ttc aac tca tcc ctg 1440 Ala Val Ala Ala Ala Leu Glu Gln Met Pro Arg Phe Asn Ser Ser Leu 465 470 475 480 tca gag gat gct caa cgt ctt acc tta aag aag tac atc aac att ggt 1488 Ser Glu Asp Ala Gln Arg Leu Thr Leu Lys Lys Tyr Ile Asn Ile Gly 485 490 495 gtt gct gtg gac acg cca aat ggg ttg gta gtc ccc gtg ttt aaa gac 1536 Val Ala Val Asp Thr Pro Asn Gly Leu Val Val Pro Val Phe Lys Asp 500 505 510 gtt aat aag aag tcc att aca gag tta tcg cgc gaa tta act gtt atc 1584 Val Asn Lys Lys Ser Ile Thr Glu Leu Ser Arg Glu Leu Thr Val Ile 515 520 525 agc aag aaa gca cgc gat ggg aag ctg act gcc ggc gaa atg caa ggc 1632 Ser Lys Lys Ala Arg Asp Gly Lys Leu Thr Ala Gly Glu Met Gln Gly 530 535 540 gga tgt ttt acc atc tcg agt att ggg gga tta ggg acc aca cat ttt 1680 Gly Cys Phe Thr Ile Ser Ser Ile Gly Gly Leu Gly Thr Thr His Phe 545 550 555 560 gca ccc atc gtc aat gca cct gaa gta gct atc tta ggg gta tca aaa 1728 Ala Pro Ile Val Asn Ala Pro Glu Val Ala Ile Leu Gly Val Ser Lys 565 570 575 tca gcg atg gag ccg gtt tgg aat ggg aaa gag ttc gta ccc cgt ctg 1776 Ser Ala Met Glu Pro Val Trp Asn Gly Lys Glu Phe Val Pro Arg Leu 580 585 590 atg atg cca att tca ctg tca ttc gac cat cgc gtc att gat ggc gcg 1824 Met Met Pro Ile Ser Leu Ser Phe Asp His Arg Val Ile Asp Gly Ala 595 600 605 gat ggc gca cgc ttt atc aca att atc aat aat atg ctt tca gat att 1872 Asp Gly Ala Arg Phe Ile Thr Ile Ile Asn Asn Met Leu Ser Asp Ile 610 615 620 cgc cgt tta gtc atg taa 1890 Arg Arg Leu Val Met 625 <210> 121 <211> 629 <212> PRT <213> Klebsiella oxytoca <400> 121 Met Ala Ile Glu Ile Lys Val Pro Asp Ile Gly Ala Asp Glu Val Glu 1 5 10 15 Ile Thr Glu Ile Leu Val Lys Val Gly Asp Lys Val Glu Ala Glu Gln 20 25 30 Ser Leu Ile Thr Val Glu Gly Asp Lys Ala Ser Met Glu Val Pro Ser 35 40 45 Pro Gln Ala Gly Val Val Lys Glu Ile Lys Val Ser Val Gly Asp Lys 50 55 60 Thr Glu Thr Gly Lys Leu Ile Met Ile Phe Asp Ser Ala Asp Gly Ala 65 70 75 80 Ala Ala Ala Ala Pro Ala Gln Glu Glu Lys Lys Glu Ala Ala Pro Ala 85 90 95 Ala Ala Ala Pro Ala Ala Ala Ser Ala Lys Glu Val His Val Pro Asp 100 105 110 Ile Gly Gly Asp Glu Val Glu Val Thr Glu Ile Met Val Lys Val Gly 115 120 125 Asp Thr Ile Ala Ala Glu Gln Ser Leu Ile Thr Val Glu Gly Asp Lys 130 135 140 Ala Ser Met Glu Val Pro Ala Pro Phe Ala Gly Thr Val Lys Glu Ile 145 150 155 160 Lys Ile Asn Thr Gly Asp Lys Val Ser Thr Gly Ser Leu Ile Met Ile 165 170 175 Phe Glu Val Ala Gly Ala Ala Pro Ala Ala Ala Pro Ala Lys Ala Glu 180 185 190 Ala Ala Pro Ala Ala Ala Ala Pro Ala Ala Ser Gly Ser Lys Glu Val 195 200 205 His Val Pro Asp Ile Gly Gly Asp Glu Val Glu Val Thr Glu Val Met 210 215 220 Val Lys Ala Gly Asp Lys Ile Ala Ala Glu Gln Ser Leu Ile Thr Val 225 230 235 240 Glu Gly Asp Lys Ala Ser Met Glu Val Pro Ala Pro Phe Ala Gly Thr 245 250 255 Val Lys Glu Ile Lys Ile Ser Thr Gly Asp Lys Val Ser Thr Gly Ser 260 265 270 Leu Ile Met Val Phe Glu Val Glu Gly Ala Ala Pro Ala Ala Ala Pro 275 280 285 Ala Ala Ala Ala Ala Pro Ala Pro Ala Ala Ala Pro Ala Gln Ala Ala 290 295 300 Lys Pro Ala Ala Pro Ala Ala Lys Ala Glu Gly Lys Ser Glu Phe Ala 305 310 315 320 Glu Asn Asp Ala Tyr Val His Ala Thr Pro Leu Ile Arg Arg Leu Ala 325 330 335 Arg Glu Phe Gly Val Asn Leu Ala Lys Val Lys Gly Thr Gly Arg Lys 340 345 350 Gly Arg Ile Leu Arg Glu Asp Val Gln Ala Tyr Val Lys Glu Ala Val 355 360 365 Lys Arg Ala Glu Ala Ala Pro Ala Ala Thr Gly Gly Gly Ile Pro Gly 370 375 380 Met Leu Pro Trp Pro Lys Val Asp Phe Ser Lys Phe Gly Glu Val Glu 385 390 395 400 Glu Val Glu Leu Gly Arg Ile Gln Lys Ile Ser Gly Ala Asn Leu Ser 405 410 415 Arg Asn Trp Val Met Ile Pro His Val Thr His Phe Asp Lys Thr Asp 420 425 430 Ile Thr Asp Leu Glu Ala Phe Arg Lys Gln Gln Asn Ala Glu Ala Glu 435 440 445 Lys Arg Lys Leu Asp Val Lys Phe Thr Pro Val Val Phe Ile Met Lys 450 455 460 Ala Val Ala Ala Ala Leu Glu Gln Met Pro Arg Phe Asn Ser Ser Leu 465 470 475 480 Ser Glu Asp Ala Gln Arg Leu Thr Leu Lys Lys Tyr Ile Asn Ile Gly 485 490 495 Val Ala Val Asp Thr Pro Asn Gly Leu Val Val Pro Val Phe Lys Asp 500 505 510 Val Asn Lys Lys Ser Ile Thr Glu Leu Ser Arg Glu Leu Thr Val Ile 515 520 525 Ser Lys Lys Ala Arg Asp Gly Lys Leu Thr Ala Gly Glu Met Gln Gly 530 535 540 Gly Cys Phe Thr Ile Ser Ser Ile Gly Gly Leu Gly Thr Thr His Phe 545 550 555 560 Ala Pro Ile Val Asn Ala Pro Glu Val Ala Ile Leu Gly Val Ser Lys 565 570 575 Ser Ala Met Glu Pro Val Trp Asn Gly Lys Glu Phe Val Pro Arg Leu 580 585 590 Met Met Pro Ile Ser Leu Ser Phe Asp His Arg Val Ile Asp Gly Ala 595 600 605 Asp Gly Ala Arg Phe Ile Thr Ile Ile Asn Asn Met Leu Ser Asp Ile 610 615 620 Arg Arg Leu Val Met 625 <210> 122 <211> 1017 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(1017) <223> lplA gene encoding lipoate-protein ligase A (LplA) <400> 122 atg tcc aca tta cgc ctg ctc atc tct gac tct tac gac ccg tgg ttt 48 Met Ser Thr Leu Arg Leu Leu Ile Ser Asp Ser Tyr Asp Pro Trp Phe 1 5 10 15 aac ctg gcg gtg gaa gag tgt att ttt cgc caa atg ccc gcc acg cag 96 Asn Leu Ala Val Glu Glu Cys Ile Phe Arg Gln Met Pro Ala Thr Gln 20 25 30 cgc gtt ctg ttt ctc tgg cgc aat gcc gac acg gta gta att ggt cgc 144 Arg Val Leu Phe Leu Trp Arg Asn Ala Asp Thr Val Val Ile Gly Arg 35 40 45 gcg cag aac ccg tgg aaa gag tgt aat acc cgg cgg atg gaa gaa gat 192 Ala Gln Asn Pro Trp Lys Glu Cys Asn Thr Arg Arg Met Glu Glu Asp 50 55 60 aac gtc cgc ctg gcg cgg cgc agt agc ggt ggc ggc gcg gtg ttc cac 240 Asn Val Arg Leu Ala Arg Arg Ser Ser Gly Gly Gly Ala Val Phe His 65 70 75 80 gat ctc ggc aat acc tgc ttt acc ttt atg gct ggc aag ccg gag tac 288 Asp Leu Gly Asn Thr Cys Phe Thr Phe Met Ala Gly Lys Pro Glu Tyr 85 90 95 gat aaa act atc tcc acg tcg att gtg ctc aat gcg ctg aac gcg ctc 336 Asp Lys Thr Ile Ser Thr Ser Ile Val Leu Asn Ala Leu Asn Ala Leu 100 105 110 ggc gtc agc gcc gaa gcg tcc gga cgt aac gat ctg gtg gtg aaa acc 384 Gly Val Ser Ala Glu Ala Ser Gly Arg Asn Asp Leu Val Val Lys Thr 115 120 125 gtc gaa ggc gac cgc aaa gtc tca ggc tcg gcc tat cgc gaa acc aaa 432 Val Glu Gly Asp Arg Lys Val Ser Gly Ser Ala Tyr Arg Glu Thr Lys 130 135 140 gat cgc ggc ttc cac cac ggc acc ttg cta ctc aat gcc gac ctc agc 480 Asp Arg Gly Phe His His Gly Thr Leu Leu Leu Asn Ala Asp Leu Ser 145 150 155 160 cgc ctg gca aac tat ctc aat ccg gat aaa aag aaa ctg gcg gcg aaa 528 Arg Leu Ala Asn Tyr Leu Asn Pro Asp Lys Lys Lys Leu Ala Ala Lys 165 170 175 ggc att acg tcg gta cgt tcc cgc gtg acc aac ctc acc gag ctg ttg 576 Gly Ile Thr Ser Val Arg Ser Arg Val Thr Asn Leu Thr Glu Leu Leu 180 185 190 ccg ggg atc acc cat gag cag gtt tgc gag gcc ata acc gag gcc ttt 624 Pro Gly Ile Thr His Glu Gln Val Cys Glu Ala Ile Thr Glu Ala Phe 195 200 205 ttc gcc cat tat ggc gag cgc gtg gaa gcg gaa atc atc tcc ccg aac 672 Phe Ala His Tyr Gly Glu Arg Val Glu Ala Glu Ile Ile Ser Pro Asn 210 215 220 aaa acg cca gac ttg cca aac ttc gcc gaa acc ttt gcc cgc cag agt 720 Lys Thr Pro Asp Leu Pro Asn Phe Ala Glu Thr Phe Ala Arg Gln Ser 225 230 235 240 agc tgg gaa tgg aac ttc ggt cag gct ccg gca ttc tcg cat ctg ctg 768 Ser Trp Glu Trp Asn Phe Gly Gln Ala Pro Ala Phe Ser His Leu Leu 245 250 255 gat gaa cgc ttt acc tgg ggc ggc gtg gaa ctg cat ttc gac gtt gaa 816 Asp Glu Arg Phe Thr Trp Gly Gly Val Glu Leu His Phe Asp Val Glu 260 265 270 aaa ggc cat atc acc cgc gcc cag gtg ttt acc gac agc ctc aac ccc 864 Lys Gly His Ile Thr Arg Ala Gln Val Phe Thr Asp Ser Leu Asn Pro 275 280 285 gcg ccg ctg gaa gcc ctc gcc gga cga ctg caa ggc tgc ctg tac cgc 912 Ala Pro Leu Glu Ala Leu Ala Gly Arg Leu Gln Gly Cys Leu Tyr Arg 290 295 300 gca gat atg ctg caa cag gag tgc gaa gcg ctg ttg gtt gac ttc ccg 960 Ala Asp Met Leu Gln Gln Glu Cys Glu Ala Leu Leu Val Asp Phe Pro 305 310 315 320 gaa cag gaa aaa gag cta cgg gag tta tcg gca tgg atg gcg ggg gct 1008 Glu Gln Glu Lys Glu Leu Arg Glu Leu Ser Ala Trp Met Ala Gly Ala 325 330 335 gta agg tag 1017 Val Arg <210> 123 <211> 338 <212> PRT <213> Escherichia coli <400> 123 Met Ser Thr Leu Arg Leu Leu Ile Ser Asp Ser Tyr Asp Pro Trp Phe 1 5 10 15 Asn Leu Ala Val Glu Glu Cys Ile Phe Arg Gln Met Pro Ala Thr Gln 20 25 30 Arg Val Leu Phe Leu Trp Arg Asn Ala Asp Thr Val Val Ile Gly Arg 35 40 45 Ala Gln Asn Pro Trp Lys Glu Cys Asn Thr Arg Arg Met Glu Glu Asp 50 55 60 Asn Val Arg Leu Ala Arg Arg Ser Ser Gly Gly Gly Ala Val Phe His 65 70 75 80 Asp Leu Gly Asn Thr Cys Phe Thr Phe Met Ala Gly Lys Pro Glu Tyr 85 90 95 Asp Lys Thr Ile Ser Thr Ser Ile Val Leu Asn Ala Leu Asn Ala Leu 100 105 110 Gly Val Ser Ala Glu Ala Ser Gly Arg Asn Asp Leu Val Val Lys Thr 115 120 125 Val Glu Gly Asp Arg Lys Val Ser Gly Ser Ala Tyr Arg Glu Thr Lys 130 135 140 Asp Arg Gly Phe His His Gly Thr Leu Leu Leu Asn Ala Asp Leu Ser 145 150 155 160 Arg Leu Ala Asn Tyr Leu Asn Pro Asp Lys Lys Lys Leu Ala Ala Lys 165 170 175 Gly Ile Thr Ser Val Arg Ser Arg Val Thr Asn Leu Thr Glu Leu Leu 180 185 190 Pro Gly Ile Thr His Glu Gln Val Cys Glu Ala Ile Thr Glu Ala Phe 195 200 205 Phe Ala His Tyr Gly Glu Arg Val Glu Ala Glu Ile Ile Ser Pro Asn 210 215 220 Lys Thr Pro Asp Leu Pro Asn Phe Ala Glu Thr Phe Ala Arg Gln Ser 225 230 235 240 Ser Trp Glu Trp Asn Phe Gly Gln Ala Pro Ala Phe Ser His Leu Leu 245 250 255 Asp Glu Arg Phe Thr Trp Gly Gly Val Glu Leu His Phe Asp Val Glu 260 265 270 Lys Gly His Ile Thr Arg Ala Gln Val Phe Thr Asp Ser Leu Asn Pro 275 280 285 Ala Pro Leu Glu Ala Leu Ala Gly Arg Leu Gln Gly Cys Leu Tyr Arg 290 295 300 Ala Asp Met Leu Gln Gln Glu Cys Glu Ala Leu Leu Val Asp Phe Pro 305 310 315 320 Glu Gln Glu Lys Glu Leu Arg Glu Leu Ser Ala Trp Met Ala Gly Ala 325 330 335 Val Arg <210> 124 <211> 1017 <212> DNA <213> Klebsiella oxytoca <220> <221> CDS <222> (1)..(1017) <223> lplA gene encoding lipoate-protein ligase A (LplA) <400> 124 atg tca acc ttg cgt ttg ctg tta tcc gac agt tat gac cca tgg ttt 48 Met Ser Thr Leu Arg Leu Leu Leu Ser Asp Ser Tyr Asp Pro Trp Phe 1 5 10 15 aac ctt gcc gta gag gag tcc att ttc cgc cag atg cca gcg aca cag 96 Asn Leu Ala Val Glu Glu Ser Ile Phe Arg Gln Met Pro Ala Thr Gln 20 25 30 cgt gtc ttg ttt ttg tgg cgt aac gcc gat acc gta gtt atc gga cgc 144 Arg Val Leu Phe Leu Trp Arg Asn Ala Asp Thr Val Val Ile Gly Arg 35 40 45 gca cag aat cca tgg aag gag tgt aac aca cgt cgc atg gag gag gac 192 Ala Gln Asn Pro Trp Lys Glu Cys Asn Thr Arg Arg Met Glu Glu Asp 50 55 60 aat gtg cgt ctg gct cgt cgc tct tcc ggg gga ggt gct gtg ttt cat 240 Asn Val Arg Leu Ala Arg Arg Ser Ser Gly Gly Gly Ala Val Phe His 65 70 75 80 gac ctt ggc aat acc tgc ttt aca ttc atg gcg ggg aag cct gaa tac 288 Asp Leu Gly Asn Thr Cys Phe Thr Phe Met Ala Gly Lys Pro Glu Tyr 85 90 95 gac aaa aca gtg tct acg aac atc gtc ctg act gcg ctg aac gcg tta 336 Asp Lys Thr Val Ser Thr Asn Ile Val Leu Thr Ala Leu Asn Ala Leu 100 105 110 ggg gtt gct gca gaa gcg tct ggg cgt aat gat tta gta gtc aag act 384 Gly Val Ala Ala Glu Ala Ser Gly Arg Asn Asp Leu Val Val Lys Thr 115 120 125 gct gag gga gac cgc aag gtt tcg ggt tca gcg tac cgc gaa aca atg 432 Ala Glu Gly Asp Arg Lys Val Ser Gly Ser Ala Tyr Arg Glu Thr Met 130 135 140 gat cgt ggc ttt cat cat ggt act ttg ctg tta aat gcg gat ctt tcc 480 Asp Arg Gly Phe His His Gly Thr Leu Leu Leu Asn Ala Asp Leu Ser 145 150 155 160 cgc ctg gcg aac tac ttg aac ccg gac aag aaa aaa ctt caa gca aag 528 Arg Leu Ala Asn Tyr Leu Asn Pro Asp Lys Lys Lys Leu Gln Ala Lys 165 170 175 ggc atc aca tcg gtc cgc ggc cgt gtc gct aat ctt gtc gag ctg tta 576 Gly Ile Thr Ser Val Arg Gly Arg Val Ala Asn Leu Val Glu Leu Leu 180 185 190 cca ggc atc acc cac cag caa gta tgc gag gcc atc cag gaa gca ttc 624 Pro Gly Ile Thr His Gln Gln Val Cys Glu Ala Ile Gln Glu Ala Phe 195 200 205 ttc gcc cac tat ggc gag cgc gtg gag gca gag gta atc tca cct gaa 672 Phe Ala His Tyr Gly Glu Arg Val Glu Ala Glu Val Ile Ser Pro Glu 210 215 220 aaa atg cct gac ctg cct aat ttc gcc gct acc ttc gct cgc cag tcg 720 Lys Met Pro Asp Leu Pro Asn Phe Ala Ala Thr Phe Ala Arg Gln Ser 225 230 235 240 tcg tgg gaa tgg aac ttc ggc caa gct cca gcc ttt agt cac ctg ctt 768 Ser Trp Glu Trp Asn Phe Gly Gln Ala Pro Ala Phe Ser His Leu Leu 245 250 255 gac gaa cgc ttc aca tgg gga ggc gtt gag ctt cat ttc gac gtg gag 816 Asp Glu Arg Phe Thr Trp Gly Gly Val Glu Leu His Phe Asp Val Glu 260 265 270 aag gga cac atc aca cgc acc cag att ttt acc gac agc ctt aac cca 864 Lys Gly His Ile Thr Arg Thr Gln Ile Phe Thr Asp Ser Leu Asn Pro 275 280 285 gca ccg ctg gag gct ttg gcc gcc cgc tta caa ggt tgc ctt tat cgc 912 Ala Pro Leu Glu Ala Leu Ala Ala Arg Leu Gln Gly Cys Leu Tyr Arg 290 295 300 gcc gac atg ctg caa caa gag tgc gat gct ctg tta gta gac ttt cca 960 Ala Asp Met Leu Gln Gln Glu Cys Asp Ala Leu Leu Val Asp Phe Pro 305 310 315 320 gag cag gag aaa gca tta cgt gag ctg tca gcc tgg att gct ggt gca 1008 Glu Gln Glu Lys Ala Leu Arg Glu Leu Ser Ala Trp Ile Ala Gly Ala 325 330 335 gta cgt taa 1017 Val Arg <210> 125 <211> 338 <212> PRT <213> Klebsiella oxytoca <400> 125 Met Ser Thr Leu Arg Leu Leu Leu Ser Asp Ser Tyr Asp Pro Trp Phe 1 5 10 15 Asn Leu Ala Val Glu Glu Ser Ile Phe Arg Gln Met Pro Ala Thr Gln 20 25 30 Arg Val Leu Phe Leu Trp Arg Asn Ala Asp Thr Val Val Ile Gly Arg 35 40 45 Ala Gln Asn Pro Trp Lys Glu Cys Asn Thr Arg Arg Met Glu Glu Asp 50 55 60 Asn Val Arg Leu Ala Arg Arg Ser Ser Gly Gly Gly Ala Val Phe His 65 70 75 80 Asp Leu Gly Asn Thr Cys Phe Thr Phe Met Ala Gly Lys Pro Glu Tyr 85 90 95 Asp Lys Thr Val Ser Thr Asn Ile Val Leu Thr Ala Leu Asn Ala Leu 100 105 110 Gly Val Ala Ala Glu Ala Ser Gly Arg Asn Asp Leu Val Val Lys Thr 115 120 125 Ala Glu Gly Asp Arg Lys Val Ser Gly Ser Ala Tyr Arg Glu Thr Met 130 135 140 Asp Arg Gly Phe His His Gly Thr Leu Leu Leu Asn Ala Asp Leu Ser 145 150 155 160 Arg Leu Ala Asn Tyr Leu Asn Pro Asp Lys Lys Lys Leu Gln Ala Lys 165 170 175 Gly Ile Thr Ser Val Arg Gly Arg Val Ala Asn Leu Val Glu Leu Leu 180 185 190 Pro Gly Ile Thr His Gln Gln Val Cys Glu Ala Ile Gln Glu Ala Phe 195 200 205 Phe Ala His Tyr Gly Glu Arg Val Glu Ala Glu Val Ile Ser Pro Glu 210 215 220 Lys Met Pro Asp Leu Pro Asn Phe Ala Ala Thr Phe Ala Arg Gln Ser 225 230 235 240 Ser Trp Glu Trp Asn Phe Gly Gln Ala Pro Ala Phe Ser His Leu Leu 245 250 255 Asp Glu Arg Phe Thr Trp Gly Gly Val Glu Leu His Phe Asp Val Glu 260 265 270 Lys Gly His Ile Thr Arg Thr Gln Ile Phe Thr Asp Ser Leu Asn Pro 275 280 285 Ala Pro Leu Glu Ala Leu Ala Ala Arg Leu Gln Gly Cys Leu Tyr Arg 290 295 300 Ala Asp Met Leu Gln Gln Glu Cys Asp Ala Leu Leu Val Asp Phe Pro 305 310 315 320 Glu Gln Glu Lys Ala Leu Arg Glu Leu Ser Ala Trp Ile Ala Gly Ala 325 330 335 Val Arg <210> 126 <211> 1854 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (1)..(1854) <223> Arabidopsis thaliana gene encoding TMP phosphatase [AT5G32470.1] <400> 126 atg cgc ttc ctc ttc ccc acg cgc ctc atc aac aac tca tct ctc ggt 48 Met Arg Phe Leu Phe Pro Thr Arg Leu Ile Asn Asn Ser Ser Leu Gly 1 5 10 15 ctc ctc cga tct cca cac acc acc gcg ccg atc cgt tct ctc tgg ttt 96 Leu Leu Arg Ser Pro His Thr Thr Ala Pro Ile Arg Ser Leu Trp Phe 20 25 30 cgc acc aag tct ccg gtc ttc cga tcg gcg act act cca ata atg acg 144 Arg Thr Lys Ser Pro Val Phe Arg Ser Ala Thr Thr Pro Ile Met Thr 35 40 45 gcg gtc gct ttc tct tca tcg ttg tcg att ccc cct acc tcg gaa gaa 192 Ala Val Ala Phe Ser Ser Ser Leu Ser Ile Pro Pro Thr Ser Glu Glu 50 55 60 gca ctt cca ggg aag cta tgg atc aag ttt aac aga gag tgt ctc ttc 240 Ala Leu Pro Gly Lys Leu Trp Ile Lys Phe Asn Arg Glu Cys Leu Phe 65 70 75 80 tct atc tat agc ccc ttc gcc gtc tgt tta gcc gcc gga aat ctc aag 288 Ser Ile Tyr Ser Pro Phe Ala Val Cys Leu Ala Ala Gly Asn Leu Lys 85 90 95 atc gac aca ttt cgt cag tat att gca cag gat gtt cat ttc ctt aag 336 Ile Asp Thr Phe Arg Gln Tyr Ile Ala Gln Asp Val His Phe Leu Lys 100 105 110 gcc ttt gct cac gcg tat gaa ctg gcc gca gat tgt gct gat gac gat 384 Ala Phe Ala His Ala Tyr Glu Leu Ala Ala Asp Cys Ala Asp Asp Asp 115 120 125 gat gat aaa ttg gca att tct gat ttg agg aaa agc gtg atg gaa gaa 432 Asp Asp Lys Leu Ala Ile Ser Asp Leu Arg Lys Ser Val Met Glu Glu 130 135 140 ttg aaa atg cac gac tca ttt gta cag gat tgg gat tta gac atc aac 480 Leu Lys Met His Asp Ser Phe Val Gln Asp Trp Asp Leu Asp Ile Asn 145 150 155 160 aaa gaa gta agt gtt aac tca gca act ttg aga tac act gag ttc ttg 528 Lys Glu Val Ser Val Asn Ser Ala Thr Leu Arg Tyr Thr Glu Phe Leu 165 170 175 tta gct aca gca tcc gga aaa gta gaa gga tgc aaa gct ccc ggc atg 576 Leu Ala Thr Ala Ser Gly Lys Val Glu Gly Cys Lys Ala Pro Gly Met 180 185 190 ctt gat act cca ttt gaa aaa aca aaa gtt gct gcc tac acg ctt ggt 624 Leu Asp Thr Pro Phe Glu Lys Thr Lys Val Ala Ala Tyr Thr Leu Gly 195 200 205 gct gtg aca cct tgc atg cgg ttg tat gcc ttt ctc ggt aag gag ttt 672 Ala Val Thr Pro Cys Met Arg Leu Tyr Ala Phe Leu Gly Lys Glu Phe 210 215 220 gga tca ctt ctt gat ctg agt gat gtg aac cat ccc tac aag aaa tgg 720 Gly Ser Leu Leu Asp Leu Ser Asp Val Asn His Pro Tyr Lys Lys Trp 225 230 235 240 atc gat aat tat tct agt gat gct ttc cag gca tca gcc aag caa act 768 Ile Asp Asn Tyr Ser Ser Asp Ala Phe Gln Ala Ser Ala Lys Gln Thr 245 250 255 gaa gac ttg ctt gag aag ctt agt gtc tct atg act ggt gaa gaa ttg 816 Glu Asp Leu Leu Glu Lys Leu Ser Val Ser Met Thr Gly Glu Glu Leu 260 265 270 gac ata att gaa aaa ttg tat caa cag gct atg aaa ctt gaa gta gag 864 Asp Ile Ile Glu Lys Leu Tyr Gln Gln Ala Met Lys Leu Glu Val Glu 275 280 285 ttc ttc cat gcc cag cca ctt gcc cag cct acc ata gtt cca ctg ctc 912 Phe Phe His Ala Gln Pro Leu Ala Gln Pro Thr Ile Val Pro Leu Leu 290 295 300 aag aac cac tca aaa gat gat ctg gtg atc ttt tct gat ttt gat ctg 960 Lys Asn His Ser Lys Asp Asp Leu Val Ile Phe Ser Asp Phe Asp Leu 305 310 315 320 act tgc acc gtt gtg gat tct tct gct att tta gcg gaa ata gca att 1008 Thr Cys Thr Val Val Asp Ser Ser Ala Ile Leu Ala Glu Ile Ala Ile 325 330 335 gta act gcc cca aaa gat gaa caa agt cga tct gga caa caa att cat 1056 Val Thr Ala Pro Lys Asp Glu Gln Ser Arg Ser Gly Gln Gln Ile His 340 345 350 cgg atg ctc tca tct gac ctt aag aac acc tgg aat cta ctt tct aaa 1104 Arg Met Leu Ser Ser Asp Leu Lys Asn Thr Trp Asn Leu Leu Ser Lys 355 360 365 caa tac aca gag cat tat gaa gaa tgc ata gag agt att ctg aat aaa 1152 Gln Tyr Thr Glu His Tyr Glu Glu Cys Ile Glu Ser Ile Leu Asn Lys 370 375 380 aag aaa gcg gac aag ttt gac tat gaa ggt tta tgt aaa gca cta gag 1200 Lys Lys Ala Asp Lys Phe Asp Tyr Glu Gly Leu Cys Lys Ala Leu Glu 385 390 395 400 cag ctt tca gat ttt gag aaa gag gca aat aat cga gtg att gag tct 1248 Gln Leu Ser Asp Phe Glu Lys Glu Ala Asn Asn Arg Val Ile Glu Ser 405 410 415 ggt gta ctc aaa ggc ctg aat ctt gaa gac att aag cgc gct ggg gaa 1296 Gly Val Leu Lys Gly Leu Asn Leu Glu Asp Ile Lys Arg Ala Gly Glu 420 425 430 agg tta atc ctt caa gat gga tgc atc aat gtc ttc cag aaa att tta 1344 Arg Leu Ile Leu Gln Asp Gly Cys Ile Asn Val Phe Gln Lys Ile Leu 435 440 445 aag act gag aat ctg aat gca gaa ctt cat gtg ctt tcc tat tgt tgg 1392 Lys Thr Glu Asn Leu Asn Ala Glu Leu His Val Leu Ser Tyr Cys Trp 450 455 460 tgt ggt gac ctc atc agg gca gcc ttt tct gca ggc gga gta gat gca 1440 Cys Gly Asp Leu Ile Arg Ala Ala Phe Ser Ala Gly Gly Val Asp Ala 465 470 475 480 gtg gaa gta cat gca aat gaa ttc aca ttt gag gaa tcc atc tcg act 1488 Val Glu Val His Ala Asn Glu Phe Thr Phe Glu Glu Ser Ile Ser Thr 485 490 495 ggt gag atc gaa aga aag gtg gaa tcc cca att aac aaa gct caa cag 1536 Gly Glu Ile Glu Arg Lys Val Glu Ser Pro Ile Asn Lys Ala Gln Gln 500 505 510 ttc aaa agt atc cta caa aac aga aag aat gag aac aat aag aaa agt 1584 Phe Lys Ser Ile Leu Gln Asn Arg Lys Asn Glu Asn Asn Lys Lys Ser 515 520 525 ttc ttg agt gtg tat att gga gat tcg gta ggt gac ttg ctg tgt ctc 1632 Phe Leu Ser Val Tyr Ile Gly Asp Ser Val Gly Asp Leu Leu Cys Leu 530 535 540 ctc gaa gca gat ata gga ata gtg gtt agc tct agc tcg agt ctc agg 1680 Leu Glu Ala Asp Ile Gly Ile Val Val Ser Ser Ser Ser Ser Leu Arg 545 550 555 560 aga gtt gga agc cat ttt ggg gtc tca ttt gtg cct ttg ttt tct gga 1728 Arg Val Gly Ser His Phe Gly Val Ser Phe Val Pro Leu Phe Ser Gly 565 570 575 atc gtc cag aaa cag aaa caa cac act gaa gaa tca tca tca tca gca 1776 Ile Val Gln Lys Gln Lys Gln His Thr Glu Glu Ser Ser Ser Ser Ala 580 585 590 tgg aaa gga ctc tct ggc aca ctt tac aca gtt tca agc tgg gcc gaa 1824 Trp Lys Gly Leu Ser Gly Thr Leu Tyr Thr Val Ser Ser Trp Ala Glu 595 600 605 att cat tca ttc gct ctt gga tgg gag taa 1854 Ile His Ser Phe Ala Leu Gly Trp Glu 610 615 <210> 127 <211> 617 <212> PRT <213> Arabidopsis thaliana <400> 127 Met Arg Phe Leu Phe Pro Thr Arg Leu Ile Asn Asn Ser Ser Leu Gly 1 5 10 15 Leu Leu Arg Ser Pro His Thr Thr Ala Pro Ile Arg Ser Leu Trp Phe 20 25 30 Arg Thr Lys Ser Pro Val Phe Arg Ser Ala Thr Thr Pro Ile Met Thr 35 40 45 Ala Val Ala Phe Ser Ser Ser Leu Ser Ile Pro Pro Thr Ser Glu Glu 50 55 60 Ala Leu Pro Gly Lys Leu Trp Ile Lys Phe Asn Arg Glu Cys Leu Phe 65 70 75 80 Ser Ile Tyr Ser Pro Phe Ala Val Cys Leu Ala Ala Gly Asn Leu Lys 85 90 95 Ile Asp Thr Phe Arg Gln Tyr Ile Ala Gln Asp Val His Phe Leu Lys 100 105 110 Ala Phe Ala His Ala Tyr Glu Leu Ala Ala Asp Cys Ala Asp Asp Asp 115 120 125 Asp Asp Lys Leu Ala Ile Ser Asp Leu Arg Lys Ser Val Met Glu Glu 130 135 140 Leu Lys Met His Asp Ser Phe Val Gln Asp Trp Asp Leu Asp Ile Asn 145 150 155 160 Lys Glu Val Ser Val Asn Ser Ala Thr Leu Arg Tyr Thr Glu Phe Leu 165 170 175 Leu Ala Thr Ala Ser Gly Lys Val Glu Gly Cys Lys Ala Pro Gly Met 180 185 190 Leu Asp Thr Pro Phe Glu Lys Thr Lys Val Ala Ala Tyr Thr Leu Gly 195 200 205 Ala Val Thr Pro Cys Met Arg Leu Tyr Ala Phe Leu Gly Lys Glu Phe 210 215 220 Gly Ser Leu Leu Asp Leu Ser Asp Val Asn His Pro Tyr Lys Lys Trp 225 230 235 240 Ile Asp Asn Tyr Ser Ser Asp Ala Phe Gln Ala Ser Ala Lys Gln Thr 245 250 255 Glu Asp Leu Leu Glu Lys Leu Ser Val Ser Met Thr Gly Glu Glu Leu 260 265 270 Asp Ile Ile Glu Lys Leu Tyr Gln Gln Ala Met Lys Leu Glu Val Glu 275 280 285 Phe Phe His Ala Gln Pro Leu Ala Gln Pro Thr Ile Val Pro Leu Leu 290 295 300 Lys Asn His Ser Lys Asp Asp Leu Val Ile Phe Ser Asp Phe Asp Leu 305 310 315 320 Thr Cys Thr Val Val Asp Ser Ser Ala Ile Leu Ala Glu Ile Ala Ile 325 330 335 Val Thr Ala Pro Lys Asp Glu Gln Ser Arg Ser Gly Gln Gln Ile His 340 345 350 Arg Met Leu Ser Ser Asp Leu Lys Asn Thr Trp Asn Leu Leu Ser Lys 355 360 365 Gln Tyr Thr Glu His Tyr Glu Glu Cys Ile Glu Ser Ile Leu Asn Lys 370 375 380 Lys Lys Ala Asp Lys Phe Asp Tyr Glu Gly Leu Cys Lys Ala Leu Glu 385 390 395 400 Gln Leu Ser Asp Phe Glu Lys Glu Ala Asn Asn Arg Val Ile Glu Ser 405 410 415 Gly Val Leu Lys Gly Leu Asn Leu Glu Asp Ile Lys Arg Ala Gly Glu 420 425 430 Arg Leu Ile Leu Gln Asp Gly Cys Ile Asn Val Phe Gln Lys Ile Leu 435 440 445 Lys Thr Glu Asn Leu Asn Ala Glu Leu His Val Leu Ser Tyr Cys Trp 450 455 460 Cys Gly Asp Leu Ile Arg Ala Ala Phe Ser Ala Gly Gly Val Asp Ala 465 470 475 480 Val Glu Val His Ala Asn Glu Phe Thr Phe Glu Glu Ser Ile Ser Thr 485 490 495 Gly Glu Ile Glu Arg Lys Val Glu Ser Pro Ile Asn Lys Ala Gln Gln 500 505 510 Phe Lys Ser Ile Leu Gln Asn Arg Lys Asn Glu Asn Asn Lys Lys Ser 515 520 525 Phe Leu Ser Val Tyr Ile Gly Asp Ser Val Gly Asp Leu Leu Cys Leu 530 535 540 Leu Glu Ala Asp Ile Gly Ile Val Val Ser Ser Ser Ser Ser Leu Arg 545 550 555 560 Arg Val Gly Ser His Phe Gly Val Ser Phe Val Pro Leu Phe Ser Gly 565 570 575 Ile Val Gln Lys Gln Lys Gln His Thr Glu Glu Ser Ser Ser Ser Ala 580 585 590 Trp Lys Gly Leu Ser Gly Thr Leu Tyr Thr Val Ser Ser Trp Ala Glu 595 600 605 Ile His Ser Phe Ala Leu Gly Trp Glu 610 615 <210> 128 <211> 1806 <212> DNA <213> Pyrus x bretschneideri <220> <221> CDS <222> (1)..(1806) <223> Pyrus x bretschneideri gene encoding TMP phosphatase [XP_009379735.1] <400> 128 atg cgc ata ctc ttc ccc cca aac cca atc aaa acc cca act ctc ttc 48 Met Arg Ile Leu Phe Pro Pro Asn Pro Ile Lys Thr Pro Thr Leu Phe 1 5 10 15 aac tcc ctc cgt ctg cga ttc aac tcg ctc cga tcc cac tgt gcc aac 96 Asn Ser Leu Arg Leu Arg Phe Asn Ser Leu Arg Ser His Cys Ala Asn 20 25 30 tca atg gcc gta cct ccg ccg aag tca gcc atg gct tcc gcc gtc gtc 144 Ser Met Ala Val Pro Pro Pro Lys Ser Ala Met Ala Ser Ala Val Val 35 40 45 ggc aac gag gtg ggt ctc gcc cgc cgc ttc tgg atc aag ttc aag cga 192 Gly Asn Glu Val Gly Leu Ala Arg Arg Phe Trp Ile Lys Phe Lys Arg 50 55 60 gaa tcg att ttc gct atg tac act ccc ttc acg ctc tgt ttg gct gct 240 Glu Ser Ile Phe Ala Met Tyr Thr Pro Phe Thr Leu Cys Leu Ala Ala 65 70 75 80 ggg aat ctc aag att gaa act ttc cgc gat tat att gcc caa gat gtt 288 Gly Asn Leu Lys Ile Glu Thr Phe Arg Asp Tyr Ile Ala Gln Asp Val 85 90 95 cac ttt ctc aag gcc ttc gct cac gcg tat gaa ttg gca gaa gat tgt 336 His Phe Leu Lys Ala Phe Ala His Ala Tyr Glu Leu Ala Glu Asp Cys 100 105 110 gca gac gat gat gat gca aag ccc gtg att tct gag ttg agg agg gca 384 Ala Asp Asp Asp Asp Ala Lys Pro Val Ile Ser Glu Leu Arg Arg Ala 115 120 125 gtt ctg cag gag ctg aaa atg cat gat tca ttt gtg aag gaa tgg ggg 432 Val Leu Gln Glu Leu Lys Met His Asp Ser Phe Val Lys Glu Trp Gly 130 135 140 tta cag ggt gct aaa gag acc cct atc aac tcc gct gcg gtg aag tac 480 Leu Gln Gly Ala Lys Glu Thr Pro Ile Asn Ser Ala Ala Val Lys Tyr 145 150 155 160 aca gat ttc tta ttg gca aca gcc tct gga aaa gtt gaa gga gtc aag 528 Thr Asp Phe Leu Leu Ala Thr Ala Ser Gly Lys Val Glu Gly Val Lys 165 170 175 gga cct ggt aaa ctt gca act cca ttt gaa aga acc aaa gtg gct gct 576 Gly Pro Gly Lys Leu Ala Thr Pro Phe Glu Arg Thr Lys Val Ala Ala 180 185 190 tac acc ctt ggc gct atg act cct tgc atg aga ctg tat gcc ttt ctt 624 Tyr Thr Leu Gly Ala Met Thr Pro Cys Met Arg Leu Tyr Ala Phe Leu 195 200 205 ggt aag gag ttc aag gca ctt cta gat ccc agc gaa ggc agt cac ccg 672 Gly Lys Glu Phe Lys Ala Leu Leu Asp Pro Ser Glu Gly Ser His Pro 210 215 220 tac ttg aag tgg att gac agt tat tct tct aaa agt ttt cag gca tca 720 Tyr Leu Lys Trp Ile Asp Ser Tyr Ser Ser Lys Ser Phe Gln Ala Ser 225 230 235 240 gct gtg caa atc gaa gag ttg ctg gat aaa cta agt gtc tct ttg aca 768 Ala Val Gln Ile Glu Glu Leu Leu Asp Lys Leu Ser Val Ser Leu Thr 245 250 255 ggc gag gag ctt gac atc atc gaa aag ctt tac cac caa gca atg aaa 816 Gly Glu Glu Leu Asp Ile Ile Glu Lys Leu Tyr His Gln Ala Met Lys 260 265 270 ctt gag atc gag ttc ttc tct gct cag tct ctt gtt cag cca act gta 864 Leu Glu Ile Glu Phe Phe Ser Ala Gln Ser Leu Val Gln Pro Thr Val 275 280 285 gtt cct ctg atc aga gaa cat aac cct gca gaa gat cgg ctc atg ata 912 Val Pro Leu Ile Arg Glu His Asn Pro Ala Glu Asp Arg Leu Met Ile 290 295 300 ttt tct gat ttt gat ttg act tgt aca gtc gtt gat tca tct gcc att 960 Phe Ser Asp Phe Asp Leu Thr Cys Thr Val Val Asp Ser Ser Ala Ile 305 310 315 320 ttg gct gaa att gca ata gta aca gca cca aaa tct gat caa cat caa 1008 Leu Ala Glu Ile Ala Ile Val Thr Ala Pro Lys Ser Asp Gln His Gln 325 330 335 ccc gaa aat cag att gct cgg atg tct tcg gct gat ctc agg aat aca 1056 Pro Glu Asn Gln Ile Ala Arg Met Ser Ser Ala Asp Leu Arg Asn Thr 340 345 350 tgg ggt ctt ctt tcc agg cag tac aca gaa gag tat gag caa tgc ata 1104 Trp Gly Leu Leu Ser Arg Gln Tyr Thr Glu Glu Tyr Glu Gln Cys Ile 355 360 365 gaa agc att gtt ccc act gaa aaa gca gtg ttt gac tat gaa aat ttg 1152 Glu Ser Ile Val Pro Thr Glu Lys Ala Val Phe Asp Tyr Glu Asn Leu 370 375 380 ctt aaa gca cta gag aaa ctt tca gat ttt gag agg aag gca aac aat 1200 Leu Lys Ala Leu Glu Lys Leu Ser Asp Phe Glu Arg Lys Ala Asn Asn 385 390 395 400 aga gtc acg aag tct gaa gta ctc aag ggt ctt aat ctc gaa gat ata 1248 Arg Val Thr Lys Ser Glu Val Leu Lys Gly Leu Asn Leu Glu Asp Ile 405 410 415 aaa aga gct ggt gaa cgt ctc att ctt caa gat ggc tgt att aat ttc 1296 Lys Arg Ala Gly Glu Arg Leu Ile Leu Gln Asp Gly Cys Ile Asn Phe 420 425 430 ttt cag aaa att gcc aag agt gaa aac ttg aat gca aat gtt cat gtt 1344 Phe Gln Lys Ile Ala Lys Ser Glu Asn Leu Asn Ala Asn Val His Val 435 440 445 ctt tca tac tgt tgg tgt ggt gat ctc ata aga tcg gcc ttt tca tca 1392 Leu Ser Tyr Cys Trp Cys Gly Asp Leu Ile Arg Ser Ala Phe Ser Ser 450 455 460 ggg ggt tta aac gag ctg gat gta cat gca aat gag ttt acc ttc gag 1440 Gly Gly Leu Asn Glu Leu Asp Val His Ala Asn Glu Phe Thr Phe Glu 465 470 475 480 gaa tcc atc tcc aca ggt gat att gtt aag aag gtg gag tcc cct att 1488 Glu Ser Ile Ser Thr Gly Asp Ile Val Lys Lys Val Glu Ser Pro Ile 485 490 495 gac aag gtt aaa tct ttt aaa gat att ttg aaa aat tgc agc aat gac 1536 Asp Lys Val Lys Ser Phe Lys Asp Ile Leu Lys Asn Cys Ser Asn Asp 500 505 510 aga aag aac ttg act gtt tac att gga gac tcg gtg ggt gac tta ctt 1584 Arg Lys Asn Leu Thr Val Tyr Ile Gly Asp Ser Val Gly Asp Leu Leu 515 520 525 tgt ctg ctg gag gcg gat att gga atc gta att ggg tca agt tca agc 1632 Cys Leu Leu Glu Ala Asp Ile Gly Ile Val Ile Gly Ser Ser Ser Ser 530 535 540 ctt agg aga gtg gcg act cag ttt ggg gta tct ttt gtt ccg ttg ttc 1680 Leu Arg Arg Val Ala Thr Gln Phe Gly Val Ser Phe Val Pro Leu Phe 545 550 555 560 ccg ggt tta gtt aag aaa cag aaa gaa tgc aca gat gga agg tct cct 1728 Pro Gly Leu Val Lys Lys Gln Lys Glu Cys Thr Asp Gly Arg Ser Pro 565 570 575 agt tgg aaa ggg tta act ggt att ctt tac aca gtg aat agt tgg gcg 1776 Ser Trp Lys Gly Leu Thr Gly Ile Leu Tyr Thr Val Asn Ser Trp Ala 580 585 590 gaa ata cat gcc ttc att ttg ggg tgt taa 1806 Glu Ile His Ala Phe Ile Leu Gly Cys 595 600 <210> 129 <211> 601 <212> PRT <213> Pyrus x bretschneideri <400> 129 Met Arg Ile Leu Phe Pro Pro Asn Pro Ile Lys Thr Pro Thr Leu Phe 1 5 10 15 Asn Ser Leu Arg Leu Arg Phe Asn Ser Leu Arg Ser His Cys Ala Asn 20 25 30 Ser Met Ala Val Pro Pro Pro Lys Ser Ala Met Ala Ser Ala Val Val 35 40 45 Gly Asn Glu Val Gly Leu Ala Arg Arg Phe Trp Ile Lys Phe Lys Arg 50 55 60 Glu Ser Ile Phe Ala Met Tyr Thr Pro Phe Thr Leu Cys Leu Ala Ala 65 70 75 80 Gly Asn Leu Lys Ile Glu Thr Phe Arg Asp Tyr Ile Ala Gln Asp Val 85 90 95 His Phe Leu Lys Ala Phe Ala His Ala Tyr Glu Leu Ala Glu Asp Cys 100 105 110 Ala Asp Asp Asp Asp Ala Lys Pro Val Ile Ser Glu Leu Arg Arg Ala 115 120 125 Val Leu Gln Glu Leu Lys Met His Asp Ser Phe Val Lys Glu Trp Gly 130 135 140 Leu Gln Gly Ala Lys Glu Thr Pro Ile Asn Ser Ala Ala Val Lys Tyr 145 150 155 160 Thr Asp Phe Leu Leu Ala Thr Ala Ser Gly Lys Val Glu Gly Val Lys 165 170 175 Gly Pro Gly Lys Leu Ala Thr Pro Phe Glu Arg Thr Lys Val Ala Ala 180 185 190 Tyr Thr Leu Gly Ala Met Thr Pro Cys Met Arg Leu Tyr Ala Phe Leu 195 200 205 Gly Lys Glu Phe Lys Ala Leu Leu Asp Pro Ser Glu Gly Ser His Pro 210 215 220 Tyr Leu Lys Trp Ile Asp Ser Tyr Ser Ser Lys Ser Phe Gln Ala Ser 225 230 235 240 Ala Val Gln Ile Glu Glu Leu Leu Asp Lys Leu Ser Val Ser Leu Thr 245 250 255 Gly Glu Glu Leu Asp Ile Ile Glu Lys Leu Tyr His Gln Ala Met Lys 260 265 270 Leu Glu Ile Glu Phe Phe Ser Ala Gln Ser Leu Val Gln Pro Thr Val 275 280 285 Val Pro Leu Ile Arg Glu His Asn Pro Ala Glu Asp Arg Leu Met Ile 290 295 300 Phe Ser Asp Phe Asp Leu Thr Cys Thr Val Val Asp Ser Ser Ala Ile 305 310 315 320 Leu Ala Glu Ile Ala Ile Val Thr Ala Pro Lys Ser Asp Gln His Gln 325 330 335 Pro Glu Asn Gln Ile Ala Arg Met Ser Ser Ala Asp Leu Arg Asn Thr 340 345 350 Trp Gly Leu Leu Ser Arg Gln Tyr Thr Glu Glu Tyr Glu Gln Cys Ile 355 360 365 Glu Ser Ile Val Pro Thr Glu Lys Ala Val Phe Asp Tyr Glu Asn Leu 370 375 380 Leu Lys Ala Leu Glu Lys Leu Ser Asp Phe Glu Arg Lys Ala Asn Asn 385 390 395 400 Arg Val Thr Lys Ser Glu Val Leu Lys Gly Leu Asn Leu Glu Asp Ile 405 410 415 Lys Arg Ala Gly Glu Arg Leu Ile Leu Gln Asp Gly Cys Ile Asn Phe 420 425 430 Phe Gln Lys Ile Ala Lys Ser Glu Asn Leu Asn Ala Asn Val His Val 435 440 445 Leu Ser Tyr Cys Trp Cys Gly Asp Leu Ile Arg Ser Ala Phe Ser Ser 450 455 460 Gly Gly Leu Asn Glu Leu Asp Val His Ala Asn Glu Phe Thr Phe Glu 465 470 475 480 Glu Ser Ile Ser Thr Gly Asp Ile Val Lys Lys Val Glu Ser Pro Ile 485 490 495 Asp Lys Val Lys Ser Phe Lys Asp Ile Leu Lys Asn Cys Ser Asn Asp 500 505 510 Arg Lys Asn Leu Thr Val Tyr Ile Gly Asp Ser Val Gly Asp Leu Leu 515 520 525 Cys Leu Leu Glu Ala Asp Ile Gly Ile Val Ile Gly Ser Ser Ser Ser 530 535 540 Leu Arg Arg Val Ala Thr Gln Phe Gly Val Ser Phe Val Pro Leu Phe 545 550 555 560 Pro Gly Leu Val Lys Lys Gln Lys Glu Cys Thr Asp Gly Arg Ser Pro 565 570 575 Ser Trp Lys Gly Leu Thr Gly Ile Leu Tyr Thr Val Asn Ser Trp Ala 580 585 590 Glu Ile His Ala Phe Ile Leu Gly Cys 595 600 <210> 130 <211> 1797 <212> DNA <213> Brassica napus <220> <221> CDS <222> (1)..(1797) <223> Brassica napus gene encoding TMP phosphatase [CDY62623.1] <400> 130 atg cgc atc ctc aac aac tcg ctc gcc ctt ctc cga tcg ccc cgc gcc 48 Met Arg Ile Leu Asn Asn Ser Leu Ala Leu Leu Arg Ser Pro Arg Ala 1 5 10 15 gcc gcc ccg atc cgt tct cta ctg ttc ggc agc aag aag tct tcc gtc 96 Ala Ala Pro Ile Arg Ser Leu Leu Phe Gly Ser Lys Lys Ser Ser Val 20 25 30 tcc cga tcg gcg gcc gcc ttc tct tcg gcg atg tcg att cct cct cct 144 Ser Arg Ser Ala Ala Ala Phe Ser Ser Ala Met Ser Ile Pro Pro Pro 35 40 45 agc ata tcc acc tcg gaa gaa gct ctg gcg ggg agg ctg tgg atc aag 192 Ser Ile Ser Thr Ser Glu Glu Ala Leu Ala Gly Arg Leu Trp Ile Lys 50 55 60 ttc aac aga gag tgc ctc ttc tct atg tac agc ccc ttc gcc gtt tct 240 Phe Asn Arg Glu Cys Leu Phe Ser Met Tyr Ser Pro Phe Ala Val Ser 65 70 75 80 ttg gcc gcc ggc aat ctc aag atc gag acc ttc cgg cag tat att gct 288 Leu Ala Ala Gly Asn Leu Lys Ile Glu Thr Phe Arg Gln Tyr Ile Ala 85 90 95 cag gat gtt cat ttc ctc aag gcc ttt gct cac gcg tat gag ttg gcc 336 Gln Asp Val His Phe Leu Lys Ala Phe Ala His Ala Tyr Glu Leu Ala 100 105 110 gca gag tgt gct gat gat gat gat gat aag ttg gca att tct gac ttg 384 Ala Glu Cys Ala Asp Asp Asp Asp Asp Lys Leu Ala Ile Ser Asp Leu 115 120 125 agg aaa agc gtc atg gat gag ttg aaa atg cac aac tca ttt gta cag 432 Arg Lys Ser Val Met Asp Glu Leu Lys Met His Asn Ser Phe Val Gln 130 135 140 gat tgg gat tta gac atc agc aaa gaa gta agt gtt aac tca gca aca 480 Asp Trp Asp Leu Asp Ile Ser Lys Glu Val Ser Val Asn Ser Ala Thr 145 150 155 160 ttg aga tac acc gag ttc tta tta gct aca tca tcc gga aaa gta gaa 528 Leu Arg Tyr Thr Glu Phe Leu Leu Ala Thr Ser Ser Gly Lys Val Glu 165 170 175 gga ctc aaa gct ccc ggc atg ctt gat act cca ttt gag aaa acc aaa 576 Gly Leu Lys Ala Pro Gly Met Leu Asp Thr Pro Phe Glu Lys Thr Lys 180 185 190 gtg gcc gcc tac acg ctt ggt gct gtg aca cct tgc atg aag ctg tat 624 Val Ala Ala Tyr Thr Leu Gly Ala Val Thr Pro Cys Met Lys Leu Tyr 195 200 205 gcc ttt ctt ggt aag gag ttt gga gcg ctt cta gat tcg agt gaa gcg 672 Ala Phe Leu Gly Lys Glu Phe Gly Ala Leu Leu Asp Ser Ser Glu Ala 210 215 220 aac cat ccc tac aag aaa tgg atc gaa aat tat tct agt gat gca ttc 720 Asn His Pro Tyr Lys Lys Trp Ile Glu Asn Tyr Ser Ser Asp Ala Phe 225 230 235 240 cag gca tca gct aag caa act gaa gac ttg ctt gag aag ctt agt gtg 768 Gln Ala Ser Ala Lys Gln Thr Glu Asp Leu Leu Glu Lys Leu Ser Val 245 250 255 tgt atg act ggc gaa gag ctg gac atc att gaa aaa ctg tat caa cag 816 Cys Met Thr Gly Glu Glu Leu Asp Ile Ile Glu Lys Leu Tyr Gln Gln 260 265 270 gca atg aaa ctt gaa gta gag ttc ttc cac gca caa ccg ttt gct cag 864 Ala Met Lys Leu Glu Val Glu Phe Phe His Ala Gln Pro Phe Ala Gln 275 280 285 cct acc ata gtt ccg ctg ctg aag aac cat tca aaa gat gag ctg atg 912 Pro Thr Ile Val Pro Leu Leu Lys Asn His Ser Lys Asp Glu Leu Met 290 295 300 ata ttt tct gat ttt gat ctg act tgc acc gtt gtt gat tct tct gct 960 Ile Phe Ser Asp Phe Asp Leu Thr Cys Thr Val Val Asp Ser Ser Ala 305 310 315 320 att tta gcc gaa att gca atc gta act gcc ccg aaa gat gat cag ggt 1008 Ile Leu Ala Glu Ile Ala Ile Val Thr Ala Pro Lys Asp Asp Gln Gly 325 330 335 caa caa att aat cgg atg ctt tcg gct gac ctt aag aac acc tgg agt 1056 Gln Gln Ile Asn Arg Met Leu Ser Ala Asp Leu Lys Asn Thr Trp Ser 340 345 350 cta ctt tcc aaa cag tat aca gag cac tat gaa gag tgc ata gag agt 1104 Leu Leu Ser Lys Gln Tyr Thr Glu His Tyr Glu Glu Cys Ile Glu Ser 355 360 365 att ctg aat aag gaa aaa gcg gac aag ttt gac tac gag ggt ttg tgt 1152 Ile Leu Asn Lys Glu Lys Ala Asp Lys Phe Asp Tyr Glu Gly Leu Cys 370 375 380 gaa gca cta gag cag ctg tca gag ttt gag aag aaa gca aac gac cga 1200 Glu Ala Leu Glu Gln Leu Ser Glu Phe Glu Lys Lys Ala Asn Asp Arg 385 390 395 400 gtg ata gag tct ggt gta ctc aag ggc ctg aat ctc gat gac atc aag 1248 Val Ile Glu Ser Gly Val Leu Lys Gly Leu Asn Leu Asp Asp Ile Lys 405 410 415 cga gct ggg gaa agg ttg att ctt caa gat ggc tgc atc aat gtc ttc 1296 Arg Ala Gly Glu Arg Leu Ile Leu Gln Asp Gly Cys Ile Asn Val Phe 420 425 430 cag aaa att ttg aag act cag gat gtg aat gca aaa ctc cac gtg ctt 1344 Gln Lys Ile Leu Lys Thr Gln Asp Val Asn Ala Lys Leu His Val Leu 435 440 445 tcg tat tgt tgg tgt ggt gac ctc atc aga gca gcc ttt tct gca cgg 1392 Ser Tyr Cys Trp Cys Gly Asp Leu Ile Arg Ala Ala Phe Ser Ala Arg 450 455 460 gga gta gat gca gtg gaa gta cat gca aat gaa ttc aca ttc gag gaa 1440 Gly Val Asp Ala Val Glu Val His Ala Asn Glu Phe Thr Phe Glu Glu 465 470 475 480 tcc atc tct act gga gaa ata gaa aga aaa gtg gaa tcc cca atc gac 1488 Ser Ile Ser Thr Gly Glu Ile Glu Arg Lys Val Glu Ser Pro Ile Asp 485 490 495 aag gct caa cag ttc aag agc atc cta caa aac aga aag aag gat gag 1536 Lys Ala Gln Gln Phe Lys Ser Ile Leu Gln Asn Arg Lys Lys Asp Glu 500 505 510 gag aaa agc atc ctc act gtt tac att gga gat tca gta ggt gac ttg 1584 Glu Lys Ser Ile Leu Thr Val Tyr Ile Gly Asp Ser Val Gly Asp Leu 515 520 525 ctc tgt ctc ctg gag gca gac att gga ata gtg gtc gcc tct agc tcg 1632 Leu Cys Leu Leu Glu Ala Asp Ile Gly Ile Val Val Ala Ser Ser Ser 530 535 540 agc ctc agg aga gtg gga agc cat ttc ggg gtc tca ttt gtg cct ttg 1680 Ser Leu Arg Arg Val Gly Ser His Phe Gly Val Ser Phe Val Pro Leu 545 550 555 560 ttc tct gga att gtg caa aaa cag aaa caa gaa gaa acc tgg aag ggg 1728 Phe Ser Gly Ile Val Gln Lys Gln Lys Gln Glu Glu Thr Trp Lys Gly 565 570 575 ctc tct ggc aca ctt tac acg gta tca agc tgg gct gaa ata cat tcc 1776 Leu Ser Gly Thr Leu Tyr Thr Val Ser Ser Trp Ala Glu Ile His Ser 580 585 590 ttc gct ctt gga tgg gag taa 1797 Phe Ala Leu Gly Trp Glu 595 <210> 131 <211> 598 <212> PRT <213> Brassica napus <400> 131 Met Arg Ile Leu Asn Asn Ser Leu Ala Leu Leu Arg Ser Pro Arg Ala 1 5 10 15 Ala Ala Pro Ile Arg Ser Leu Leu Phe Gly Ser Lys Lys Ser Ser Val 20 25 30 Ser Arg Ser Ala Ala Ala Phe Ser Ser Ala Met Ser Ile Pro Pro Pro 35 40 45 Ser Ile Ser Thr Ser Glu Glu Ala Leu Ala Gly Arg Leu Trp Ile Lys 50 55 60 Phe Asn Arg Glu Cys Leu Phe Ser Met Tyr Ser Pro Phe Ala Val Ser 65 70 75 80 Leu Ala Ala Gly Asn Leu Lys Ile Glu Thr Phe Arg Gln Tyr Ile Ala 85 90 95 Gln Asp Val His Phe Leu Lys Ala Phe Ala His Ala Tyr Glu Leu Ala 100 105 110 Ala Glu Cys Ala Asp Asp Asp Asp Asp Lys Leu Ala Ile Ser Asp Leu 115 120 125 Arg Lys Ser Val Met Asp Glu Leu Lys Met His Asn Ser Phe Val Gln 130 135 140 Asp Trp Asp Leu Asp Ile Ser Lys Glu Val Ser Val Asn Ser Ala Thr 145 150 155 160 Leu Arg Tyr Thr Glu Phe Leu Leu Ala Thr Ser Ser Gly Lys Val Glu 165 170 175 Gly Leu Lys Ala Pro Gly Met Leu Asp Thr Pro Phe Glu Lys Thr Lys 180 185 190 Val Ala Ala Tyr Thr Leu Gly Ala Val Thr Pro Cys Met Lys Leu Tyr 195 200 205 Ala Phe Leu Gly Lys Glu Phe Gly Ala Leu Leu Asp Ser Ser Glu Ala 210 215 220 Asn His Pro Tyr Lys Lys Trp Ile Glu Asn Tyr Ser Ser Asp Ala Phe 225 230 235 240 Gln Ala Ser Ala Lys Gln Thr Glu Asp Leu Leu Glu Lys Leu Ser Val 245 250 255 Cys Met Thr Gly Glu Glu Leu Asp Ile Ile Glu Lys Leu Tyr Gln Gln 260 265 270 Ala Met Lys Leu Glu Val Glu Phe Phe His Ala Gln Pro Phe Ala Gln 275 280 285 Pro Thr Ile Val Pro Leu Leu Lys Asn His Ser Lys Asp Glu Leu Met 290 295 300 Ile Phe Ser Asp Phe Asp Leu Thr Cys Thr Val Val Asp Ser Ser Ala 305 310 315 320 Ile Leu Ala Glu Ile Ala Ile Val Thr Ala Pro Lys Asp Asp Gln Gly 325 330 335 Gln Gln Ile Asn Arg Met Leu Ser Ala Asp Leu Lys Asn Thr Trp Ser 340 345 350 Leu Leu Ser Lys Gln Tyr Thr Glu His Tyr Glu Glu Cys Ile Glu Ser 355 360 365 Ile Leu Asn Lys Glu Lys Ala Asp Lys Phe Asp Tyr Glu Gly Leu Cys 370 375 380 Glu Ala Leu Glu Gln Leu Ser Glu Phe Glu Lys Lys Ala Asn Asp Arg 385 390 395 400 Val Ile Glu Ser Gly Val Leu Lys Gly Leu Asn Leu Asp Asp Ile Lys 405 410 415 Arg Ala Gly Glu Arg Leu Ile Leu Gln Asp Gly Cys Ile Asn Val Phe 420 425 430 Gln Lys Ile Leu Lys Thr Gln Asp Val Asn Ala Lys Leu His Val Leu 435 440 445 Ser Tyr Cys Trp Cys Gly Asp Leu Ile Arg Ala Ala Phe Ser Ala Arg 450 455 460 Gly Val Asp Ala Val Glu Val His Ala Asn Glu Phe Thr Phe Glu Glu 465 470 475 480 Ser Ile Ser Thr Gly Glu Ile Glu Arg Lys Val Glu Ser Pro Ile Asp 485 490 495 Lys Ala Gln Gln Phe Lys Ser Ile Leu Gln Asn Arg Lys Lys Asp Glu 500 505 510 Glu Lys Ser Ile Leu Thr Val Tyr Ile Gly Asp Ser Val Gly Asp Leu 515 520 525 Leu Cys Leu Leu Glu Ala Asp Ile Gly Ile Val Val Ala Ser Ser Ser 530 535 540 Ser Leu Arg Arg Val Gly Ser His Phe Gly Val Ser Phe Val Pro Leu 545 550 555 560 Phe Ser Gly Ile Val Gln Lys Gln Lys Gln Glu Glu Thr Trp Lys Gly 565 570 575 Leu Ser Gly Thr Leu Tyr Thr Val Ser Ser Trp Ala Glu Ile His Ser 580 585 590 Phe Ala Leu Gly Trp Glu 595 <210> 132 <211> 1815 <212> DNA <213> Glycine max <220> <221> CDS <222> (1)..(1815) <223> Glycine max gene encoding TMP phosphatase [XP_003536133.1] <400> 132 atg cgc atg cgg tgg ttc ctc cga agc cca atc atc aaa acc tcg ctg 48 Met Arg Met Arg Trp Phe Leu Arg Ser Pro Ile Ile Lys Thr Ser Leu 1 5 10 15 ctg aat ctg agc cct cca att tcg ttt aga cct cac tgg gcg agg agg 96 Leu Asn Leu Ser Pro Pro Ile Ser Phe Arg Pro His Trp Ala Arg Arg 20 25 30 acc ttc act tct tcg aga ttg tca atg gcg gcc atc cac aac cac agc 144 Thr Phe Thr Ser Ser Arg Leu Ser Met Ala Ala Ile His Asn His Ser 35 40 45 aac agc aac agc gaa acc gga ctc gcg aga cgg ttt tgg atc aag ttc 192 Asn Ser Asn Ser Glu Thr Gly Leu Ala Arg Arg Phe Trp Ile Lys Phe 50 55 60 act cgt gaa tcc atc ttc gcc atg tac act ccc ttc gcc atc gcc ttg 240 Thr Arg Glu Ser Ile Phe Ala Met Tyr Thr Pro Phe Ala Ile Ala Leu 65 70 75 80 gcc tcc ggt aat ttg cac att gat tcc ttc cac cat tac atc gcc caa 288 Ala Ser Gly Asn Leu His Ile Asp Ser Phe His His Tyr Ile Ala Gln 85 90 95 gac gtt cat ttc cta cgc gcc ttt gct caa gcg tat gag ttg gct gaa 336 Asp Val His Phe Leu Arg Ala Phe Ala Gln Ala Tyr Glu Leu Ala Glu 100 105 110 gag tgt gct gat gac gac gat gcg aaa ctt gga atc tgt gag ttg agg 384 Glu Cys Ala Asp Asp Asp Asp Ala Lys Leu Gly Ile Cys Glu Leu Arg 115 120 125 aag gca gtt cta gag gag ctg aag atg cac aac ttg ctg gta cag gaa 432 Lys Ala Val Leu Glu Glu Leu Lys Met His Asn Leu Leu Val Gln Glu 130 135 140 cgg gag ttg gac ctt gcc aaa gag cat ggt att aat tct gca act gtt 480 Arg Glu Leu Asp Leu Ala Lys Glu His Gly Ile Asn Ser Ala Thr Val 145 150 155 160 aag tac aca gag ttc ctg ctg gct aca gcc tct ggg aag att gaa gga 528 Lys Tyr Thr Glu Phe Leu Leu Ala Thr Ala Ser Gly Lys Ile Glu Gly 165 170 175 cta aaa ggt cct ggt aaa ctt gct aca cca ttt gag aaa aca aaa att 576 Leu Lys Gly Pro Gly Lys Leu Ala Thr Pro Phe Glu Lys Thr Lys Ile 180 185 190 gct gct tat act tta ggt gcc atg act cct tgc atg agg ctt tat gcc 624 Ala Ala Tyr Thr Leu Gly Ala Met Thr Pro Cys Met Arg Leu Tyr Ala 195 200 205 gtt atg gga aag aag ttc cag gaa ctt ttg gat tcc aat gaa agt act 672 Val Met Gly Lys Lys Phe Gln Glu Leu Leu Asp Ser Asn Glu Ser Thr 210 215 220 cac cca tat aac aag tgg atc aac aac tat tcc tct gat ggt ttc cag 720 His Pro Tyr Asn Lys Trp Ile Asn Asn Tyr Ser Ser Asp Gly Phe Gln 225 230 235 240 gct act act ctg caa act gaa gat ttg ctc gac aaa cta agt gtc tct 768 Ala Thr Thr Leu Gln Thr Glu Asp Leu Leu Asp Lys Leu Ser Val Ser 245 250 255 ttg act ggt gaa gaa ctt gat gtc att gaa aag ctt tat tac caa gca 816 Leu Thr Gly Glu Glu Leu Asp Val Ile Glu Lys Leu Tyr Tyr Gln Ala 260 265 270 atg aag ctt gaa ata gag ttc ttc tct gct cag cca ctc ttc cag cca 864 Met Lys Leu Glu Ile Glu Phe Phe Ser Ala Gln Pro Leu Phe Gln Pro 275 280 285 act ata gta ccc ttg act aaa gga cat aag cct gtg gaa gat cat ctc 912 Thr Ile Val Pro Leu Thr Lys Gly His Lys Pro Val Glu Asp His Leu 290 295 300 att att ttt tct gat ttt gat tta aca tgc acc gta gtt gat tcg tcc 960 Ile Ile Phe Ser Asp Phe Asp Leu Thr Cys Thr Val Val Asp Ser Ser 305 310 315 320 gcc atc ttg gct gaa att gcc ata gtg acg gca cca aaa tct gat cag 1008 Ala Ile Leu Ala Glu Ile Ala Ile Val Thr Ala Pro Lys Ser Asp Gln 325 330 335 aat cag cct gaa gat caa att gtt cgg atg tta tct tct gac ctc agg 1056 Asn Gln Pro Glu Asp Gln Ile Val Arg Met Leu Ser Ser Asp Leu Arg 340 345 350 aat aca tgg ggt ttt cta tct aaa cag tat acg gag gag tat gag caa 1104 Asn Thr Trp Gly Phe Leu Ser Lys Gln Tyr Thr Glu Glu Tyr Glu Gln 355 360 365 tgt ata gaa agc att atg cct ccc gat aga ttg aac aat ttc gat tac 1152 Cys Ile Glu Ser Ile Met Pro Pro Asp Arg Leu Asn Asn Phe Asp Tyr 370 375 380 aaa gaa ttg tcg atg gcc ctt gag caa ctt tca aaa ttt gag aac act 1200 Lys Glu Leu Ser Met Ala Leu Glu Gln Leu Ser Lys Phe Glu Asn Thr 385 390 395 400 gca aat aat agg gtt atc gag tca ggg gta ctc aag ggt ata agt cta 1248 Ala Asn Asn Arg Val Ile Glu Ser Gly Val Leu Lys Gly Ile Ser Leu 405 410 415 gaa gat ata aag cgt gct gga gag cgt ctg ata cta caa gat ggt tgc 1296 Glu Asp Ile Lys Arg Ala Gly Glu Arg Leu Ile Leu Gln Asp Gly Cys 420 425 430 cct aac ttc ttt cag agc att gtt aag aat gaa aat ttg aat gcc aac 1344 Pro Asn Phe Phe Gln Ser Ile Val Lys Asn Glu Asn Leu Asn Ala Asn 435 440 445 gtg cat gtt ctt tca tac tgc tgg tgt ggt gac ctc att agg tct act 1392 Val His Val Leu Ser Tyr Cys Trp Cys Gly Asp Leu Ile Arg Ser Thr 450 455 460 ttc tct tcc gct gat tta aat gag ttg aat gtt cat gct aat gag ttc 1440 Phe Ser Ser Ala Asp Leu Asn Glu Leu Asn Val His Ala Asn Glu Phe 465 470 475 480 act tat gag gga tct gtt tcc acg ggt gaa att gtt aag aaa gtg gag 1488 Thr Tyr Glu Gly Ser Val Ser Thr Gly Glu Ile Val Lys Lys Val Glu 485 490 495 tct ccc att gac aag gtt gaa gct ttt cgt aac ata ttg aaa aat tgc 1536 Ser Pro Ile Asp Lys Val Glu Ala Phe Arg Asn Ile Leu Lys Asn Cys 500 505 510 aat gat gac aaa aag aaa tta act gtt tac att ggc gat tca gtg ggt 1584 Asn Asp Asp Lys Lys Lys Leu Thr Val Tyr Ile Gly Asp Ser Val Gly 515 520 525 gat tta ctt tgc cta ctt gaa gct gat gta gga att gtg att ggt tca 1632 Asp Leu Leu Cys Leu Leu Glu Ala Asp Val Gly Ile Val Ile Gly Ser 530 535 540 agt tca agc ctt aga agt gta ggg acg cag ttt ggt att tca ttt gtc 1680 Ser Ser Ser Leu Arg Ser Val Gly Thr Gln Phe Gly Ile Ser Phe Val 545 550 555 560 cca ttg tat tct ggc ttg gtt aag aaa cag aaa gaa tat gtt gaa gga 1728 Pro Leu Tyr Ser Gly Leu Val Lys Lys Gln Lys Glu Tyr Val Glu Gly 565 570 575 agc act tct gat tgg aag ggt tta tct ggc att ctt tac aca gtc tct 1776 Ser Thr Ser Asp Trp Lys Gly Leu Ser Gly Ile Leu Tyr Thr Val Ser 580 585 590 agt tgg gct gaa gtg cat gct ttt att ttg ggt tgc tag 1815 Ser Trp Ala Glu Val His Ala Phe Ile Leu Gly Cys 595 600 <210> 133 <211> 604 <212> PRT <213> Glycine max <400> 133 Met Arg Met Arg Trp Phe Leu Arg Ser Pro Ile Ile Lys Thr Ser Leu 1 5 10 15 Leu Asn Leu Ser Pro Pro Ile Ser Phe Arg Pro His Trp Ala Arg Arg 20 25 30 Thr Phe Thr Ser Ser Arg Leu Ser Met Ala Ala Ile His Asn His Ser 35 40 45 Asn Ser Asn Ser Glu Thr Gly Leu Ala Arg Arg Phe Trp Ile Lys Phe 50 55 60 Thr Arg Glu Ser Ile Phe Ala Met Tyr Thr Pro Phe Ala Ile Ala Leu 65 70 75 80 Ala Ser Gly Asn Leu His Ile Asp Ser Phe His His Tyr Ile Ala Gln 85 90 95 Asp Val His Phe Leu Arg Ala Phe Ala Gln Ala Tyr Glu Leu Ala Glu 100 105 110 Glu Cys Ala Asp Asp Asp Asp Ala Lys Leu Gly Ile Cys Glu Leu Arg 115 120 125 Lys Ala Val Leu Glu Glu Leu Lys Met His Asn Leu Leu Val Gln Glu 130 135 140 Arg Glu Leu Asp Leu Ala Lys Glu His Gly Ile Asn Ser Ala Thr Val 145 150 155 160 Lys Tyr Thr Glu Phe Leu Leu Ala Thr Ala Ser Gly Lys Ile Glu Gly 165 170 175 Leu Lys Gly Pro Gly Lys Leu Ala Thr Pro Phe Glu Lys Thr Lys Ile 180 185 190 Ala Ala Tyr Thr Leu Gly Ala Met Thr Pro Cys Met Arg Leu Tyr Ala 195 200 205 Val Met Gly Lys Lys Phe Gln Glu Leu Leu Asp Ser Asn Glu Ser Thr 210 215 220 His Pro Tyr Asn Lys Trp Ile Asn Asn Tyr Ser Ser Asp Gly Phe Gln 225 230 235 240 Ala Thr Thr Leu Gln Thr Glu Asp Leu Leu Asp Lys Leu Ser Val Ser 245 250 255 Leu Thr Gly Glu Glu Leu Asp Val Ile Glu Lys Leu Tyr Tyr Gln Ala 260 265 270 Met Lys Leu Glu Ile Glu Phe Phe Ser Ala Gln Pro Leu Phe Gln Pro 275 280 285 Thr Ile Val Pro Leu Thr Lys Gly His Lys Pro Val Glu Asp His Leu 290 295 300 Ile Ile Phe Ser Asp Phe Asp Leu Thr Cys Thr Val Val Asp Ser Ser 305 310 315 320 Ala Ile Leu Ala Glu Ile Ala Ile Val Thr Ala Pro Lys Ser Asp Gln 325 330 335 Asn Gln Pro Glu Asp Gln Ile Val Arg Met Leu Ser Ser Asp Leu Arg 340 345 350 Asn Thr Trp Gly Phe Leu Ser Lys Gln Tyr Thr Glu Glu Tyr Glu Gln 355 360 365 Cys Ile Glu Ser Ile Met Pro Pro Asp Arg Leu Asn Asn Phe Asp Tyr 370 375 380 Lys Glu Leu Ser Met Ala Leu Glu Gln Leu Ser Lys Phe Glu Asn Thr 385 390 395 400 Ala Asn Asn Arg Val Ile Glu Ser Gly Val Leu Lys Gly Ile Ser Leu 405 410 415 Glu Asp Ile Lys Arg Ala Gly Glu Arg Leu Ile Leu Gln Asp Gly Cys 420 425 430 Pro Asn Phe Phe Gln Ser Ile Val Lys Asn Glu Asn Leu Asn Ala Asn 435 440 445 Val His Val Leu Ser Tyr Cys Trp Cys Gly Asp Leu Ile Arg Ser Thr 450 455 460 Phe Ser Ser Ala Asp Leu Asn Glu Leu Asn Val His Ala Asn Glu Phe 465 470 475 480 Thr Tyr Glu Gly Ser Val Ser Thr Gly Glu Ile Val Lys Lys Val Glu 485 490 495 Ser Pro Ile Asp Lys Val Glu Ala Phe Arg Asn Ile Leu Lys Asn Cys 500 505 510 Asn Asp Asp Lys Lys Lys Leu Thr Val Tyr Ile Gly Asp Ser Val Gly 515 520 525 Asp Leu Leu Cys Leu Leu Glu Ala Asp Val Gly Ile Val Ile Gly Ser 530 535 540 Ser Ser Ser Leu Arg Ser Val Gly Thr Gln Phe Gly Ile Ser Phe Val 545 550 555 560 Pro Leu Tyr Ser Gly Leu Val Lys Lys Gln Lys Glu Tyr Val Glu Gly 565 570 575 Ser Thr Ser Asp Trp Lys Gly Leu Ser Gly Ile Leu Tyr Thr Val Ser 580 585 590 Ser Trp Ala Glu Val His Ala Phe Ile Leu Gly Cys 595 600 <210> 134 <211> 1845 <212> DNA <213> Nicotiana tomentosiformis <220> <221> CDS <222> (1)..(1845) <223> Nicotiana tomentosiformi gene encoding TMP phosphatase [XP_009615535.1] <400> 134 atg cgc ttc tca tta tta tcg ccc ctt gtt ctt aac cca gtc atc aga 48 Met Arg Phe Ser Leu Leu Ser Pro Leu Val Leu Asn Pro Val Ile Arg 1 5 10 15 ttc tcc aat tcc aac gcg ctt ttt ggg tta cga ttc caa tta tac cct 96 Phe Ser Asn Ser Asn Ala Leu Phe Gly Leu Arg Phe Gln Leu Tyr Pro 20 25 30 cgt tac tct cgg tat tta cga tcg ccc gtt aca atg gcg tcg gcg aaa 144 Arg Tyr Ser Arg Tyr Leu Arg Ser Pro Val Thr Met Ala Ser Ala Lys 35 40 45 cca aag ccg gcg gcg gcg gtg aac aag ttt ccg gta gag gag gaa tgt 192 Pro Lys Pro Ala Ala Ala Val Asn Lys Phe Pro Val Glu Glu Glu Cys 50 55 60 gtg ggt ata gcg agg aag tgt tgg atc aag ttc aag aga gag tct act 240 Val Gly Ile Ala Arg Lys Cys Trp Ile Lys Phe Lys Arg Glu Ser Thr 65 70 75 80 ttc gct ctg tac act ccg ttt gtg gtt agt ttg gca tca gga acc cta 288 Phe Ala Leu Tyr Thr Pro Phe Val Val Ser Leu Ala Ser Gly Thr Leu 85 90 95 aat ctg gac act ttc cgc cat tac att gct cag gat gtt cac ttc ctc 336 Asn Leu Asp Thr Phe Arg His Tyr Ile Ala Gln Asp Val His Phe Leu 100 105 110 aaa tcc ttc gct caa gcg tat gaa gct gca gaa gag tgt act gac gat 384 Lys Ser Phe Ala Gln Ala Tyr Glu Ala Ala Glu Glu Cys Thr Asp Asp 115 120 125 gac gat gcg aag gtt ggc att agt gag ttg cgg aag aat gtt att gaa 432 Asp Asp Ala Lys Val Gly Ile Ser Glu Leu Arg Lys Asn Val Ile Glu 130 135 140 gaa ctt aaa atg cat gat gca gtt tta aaa gag tgg ggc att gat ctg 480 Glu Leu Lys Met His Asp Ala Val Leu Lys Glu Trp Gly Ile Asp Leu 145 150 155 160 gtc aaa gag tcc agt ctt aac cct gca acg gcc aag tac aca gat ttt 528 Val Lys Glu Ser Ser Leu Asn Pro Ala Thr Ala Lys Tyr Thr Asp Phe 165 170 175 tta tca gct aca gct tca gga aag gtg gaa gga gta aaa gct gct aaa 576 Leu Ser Ala Thr Ala Ser Gly Lys Val Glu Gly Val Lys Ala Ala Lys 180 185 190 ctt gcc aca cca ttt gag aga acg aag ttg gca gct tat act cta ggt 624 Leu Ala Thr Pro Phe Glu Arg Thr Lys Leu Ala Ala Tyr Thr Leu Gly 195 200 205 gct atg act cct tgc atg agg ctt tac gcc tac att ggt aag gag ctg 672 Ala Met Thr Pro Cys Met Arg Leu Tyr Ala Tyr Ile Gly Lys Glu Leu 210 215 220 caa gtg ttc ctc gag gga gag aaa att cat cca tac aag aag tgg att 720 Gln Val Phe Leu Glu Gly Glu Lys Ile His Pro Tyr Lys Lys Trp Ile 225 230 235 240 gac agt tat gcc tct gaa agt ttc cag gca tca gct ctt caa acc gag 768 Asp Ser Tyr Ala Ser Glu Ser Phe Gln Ala Ser Ala Leu Gln Thr Glu 245 250 255 gac ttg ttg gat aaa ctg agt gtc cct ttg aca ggc gag gag ctt gac 816 Asp Leu Leu Asp Lys Leu Ser Val Pro Leu Thr Gly Glu Glu Leu Asp 260 265 270 atc att gaa aag ctt tat cat caa gca atg aaa ctt gaa att gat ttc 864 Ile Ile Glu Lys Leu Tyr His Gln Ala Met Lys Leu Glu Ile Asp Phe 275 280 285 ttc tta acc cag cca ctt gtt cag aaa gct gtc atc cct ttg tca aaa 912 Phe Leu Thr Gln Pro Leu Val Gln Lys Ala Val Ile Pro Leu Ser Lys 290 295 300 gat cac aac cct gct gaa cac cgg ctt aca ata ttt tct gat ttc gat 960 Asp His Asn Pro Ala Glu His Arg Leu Thr Ile Phe Ser Asp Phe Asp 305 310 315 320 ttg acg tgc act gtt gtt gat tct tct gcc atc ttg gct gaa att gca 1008 Leu Thr Cys Thr Val Val Asp Ser Ser Ala Ile Leu Ala Glu Ile Ala 325 330 335 att ata aca gca ccg aga tct gat caa aat cga cca gag aat caa att 1056 Ile Ile Thr Ala Pro Arg Ser Asp Gln Asn Arg Pro Glu Asn Gln Ile 340 345 350 gcg cgg atg ttg tcg gct gat ttg agg aat aca tgg gga gat ctc tct 1104 Ala Arg Met Leu Ser Ala Asp Leu Arg Asn Thr Trp Gly Asp Leu Ser 355 360 365 aag cag tac act gaa gag tat gag caa tgt ata gag aag atg tta ctt 1152 Lys Gln Tyr Thr Glu Glu Tyr Glu Gln Cys Ile Glu Lys Met Leu Leu 370 375 380 act gaa aaa gcg gaa aaa ttt gat tat gaa aga ctg cat aaa aca ctt 1200 Thr Glu Lys Ala Glu Lys Phe Asp Tyr Glu Arg Leu His Lys Thr Leu 385 390 395 400 gag gaa ctt tct gat ttt gag aaa aga gca aat act agg gtg act gaa 1248 Glu Glu Leu Ser Asp Phe Glu Lys Arg Ala Asn Thr Arg Val Thr Glu 405 410 415 tct ggg gta ctg aaa ggt tta aac ctt gaa gac ata aaa cga gct ggg 1296 Ser Gly Val Leu Lys Gly Leu Asn Leu Glu Asp Ile Lys Arg Ala Gly 420 425 430 cag cga ttg att ctc cag gat ggt tgc acc aac ttc ttc cag agc ata 1344 Gln Arg Leu Ile Leu Gln Asp Gly Cys Thr Asn Phe Phe Gln Ser Ile 435 440 445 ata aga aat gaa aat ctg aac gca gac att cat gtc ctc tcc tat tgc 1392 Ile Arg Asn Glu Asn Leu Asn Ala Asp Ile His Val Leu Ser Tyr Cys 450 455 460 tgg tgt ggc gac ctt att agg tct tcc ttt tca tca ggg ggt ata gac 1440 Trp Cys Gly Asp Leu Ile Arg Ser Ser Phe Ser Ser Gly Gly Ile Asp 465 470 475 480 gct ctg aat gtg cat gcc aat gag ttt atg ttt caa gaa tct cta tcc 1488 Ala Leu Asn Val His Ala Asn Glu Phe Met Phe Gln Glu Ser Leu Ser 485 490 495 act ggt gaa att gtt aag aaa gtt gaa tcc ccc att gac aag gtt caa 1536 Thr Gly Glu Ile Val Lys Lys Val Glu Ser Pro Ile Asp Lys Val Gln 500 505 510 gca ttc agt aaa att cga atg aac tgt ggc aat gac caa aaa aat ctg 1584 Ala Phe Ser Lys Ile Arg Met Asn Cys Gly Asn Asp Gln Lys Asn Leu 515 520 525 act ctt tat att ggg gat tca gtc ggc gac tta ctt tgc ttg ctt gaa 1632 Thr Leu Tyr Ile Gly Asp Ser Val Gly Asp Leu Leu Cys Leu Leu Glu 530 535 540 gca gat gtt ggc ata gtg ctt ggt acg agc tca agt cta agg acg gtg 1680 Ala Asp Val Gly Ile Val Leu Gly Thr Ser Ser Ser Leu Arg Thr Val 545 550 555 560 ggg aat cat ttt ggt gtt tct ttt gtt cct ctg ttt cca ggt gtt gtc 1728 Gly Asn His Phe Gly Val Ser Phe Val Pro Leu Phe Pro Gly Val Val 565 570 575 cag aaa cag aag atg tgc act ggg gta gac tcg tca agt tgt tgg aag 1776 Gln Lys Gln Lys Met Cys Thr Gly Val Asp Ser Ser Ser Cys Trp Lys 580 585 590 gga cta tct ggt gtt ctc tat act gcc tct agc tgg gct gag ata cat 1824 Gly Leu Ser Gly Val Leu Tyr Thr Ala Ser Ser Trp Ala Glu Ile His 595 600 605 gct ttt gta ttg ggg tca tga 1845 Ala Phe Val Leu Gly Ser 610 <210> 135 <211> 614 <212> PRT <213> Nicotiana tomentosiformis <400> 135 Met Arg Phe Ser Leu Leu Ser Pro Leu Val Leu Asn Pro Val Ile Arg 1 5 10 15 Phe Ser Asn Ser Asn Ala Leu Phe Gly Leu Arg Phe Gln Leu Tyr Pro 20 25 30 Arg Tyr Ser Arg Tyr Leu Arg Ser Pro Val Thr Met Ala Ser Ala Lys 35 40 45 Pro Lys Pro Ala Ala Ala Val Asn Lys Phe Pro Val Glu Glu Glu Cys 50 55 60 Val Gly Ile Ala Arg Lys Cys Trp Ile Lys Phe Lys Arg Glu Ser Thr 65 70 75 80 Phe Ala Leu Tyr Thr Pro Phe Val Val Ser Leu Ala Ser Gly Thr Leu 85 90 95 Asn Leu Asp Thr Phe Arg His Tyr Ile Ala Gln Asp Val His Phe Leu 100 105 110 Lys Ser Phe Ala Gln Ala Tyr Glu Ala Ala Glu Glu Cys Thr Asp Asp 115 120 125 Asp Asp Ala Lys Val Gly Ile Ser Glu Leu Arg Lys Asn Val Ile Glu 130 135 140 Glu Leu Lys Met His Asp Ala Val Leu Lys Glu Trp Gly Ile Asp Leu 145 150 155 160 Val Lys Glu Ser Ser Leu Asn Pro Ala Thr Ala Lys Tyr Thr Asp Phe 165 170 175 Leu Ser Ala Thr Ala Ser Gly Lys Val Glu Gly Val Lys Ala Ala Lys 180 185 190 Leu Ala Thr Pro Phe Glu Arg Thr Lys Leu Ala Ala Tyr Thr Leu Gly 195 200 205 Ala Met Thr Pro Cys Met Arg Leu Tyr Ala Tyr Ile Gly Lys Glu Leu 210 215 220 Gln Val Phe Leu Glu Gly Glu Lys Ile His Pro Tyr Lys Lys Trp Ile 225 230 235 240 Asp Ser Tyr Ala Ser Glu Ser Phe Gln Ala Ser Ala Leu Gln Thr Glu 245 250 255 Asp Leu Leu Asp Lys Leu Ser Val Pro Leu Thr Gly Glu Glu Leu Asp 260 265 270 Ile Ile Glu Lys Leu Tyr His Gln Ala Met Lys Leu Glu Ile Asp Phe 275 280 285 Phe Leu Thr Gln Pro Leu Val Gln Lys Ala Val Ile Pro Leu Ser Lys 290 295 300 Asp His Asn Pro Ala Glu His Arg Leu Thr Ile Phe Ser Asp Phe Asp 305 310 315 320 Leu Thr Cys Thr Val Val Asp Ser Ser Ala Ile Leu Ala Glu Ile Ala 325 330 335 Ile Ile Thr Ala Pro Arg Ser Asp Gln Asn Arg Pro Glu Asn Gln Ile 340 345 350 Ala Arg Met Leu Ser Ala Asp Leu Arg Asn Thr Trp Gly Asp Leu Ser 355 360 365 Lys Gln Tyr Thr Glu Glu Tyr Glu Gln Cys Ile Glu Lys Met Leu Leu 370 375 380 Thr Glu Lys Ala Glu Lys Phe Asp Tyr Glu Arg Leu His Lys Thr Leu 385 390 395 400 Glu Glu Leu Ser Asp Phe Glu Lys Arg Ala Asn Thr Arg Val Thr Glu 405 410 415 Ser Gly Val Leu Lys Gly Leu Asn Leu Glu Asp Ile Lys Arg Ala Gly 420 425 430 Gln Arg Leu Ile Leu Gln Asp Gly Cys Thr Asn Phe Phe Gln Ser Ile 435 440 445 Ile Arg Asn Glu Asn Leu Asn Ala Asp Ile His Val Leu Ser Tyr Cys 450 455 460 Trp Cys Gly Asp Leu Ile Arg Ser Ser Phe Ser Ser Gly Gly Ile Asp 465 470 475 480 Ala Leu Asn Val His Ala Asn Glu Phe Met Phe Gln Glu Ser Leu Ser 485 490 495 Thr Gly Glu Ile Val Lys Lys Val Glu Ser Pro Ile Asp Lys Val Gln 500 505 510 Ala Phe Ser Lys Ile Arg Met Asn Cys Gly Asn Asp Gln Lys Asn Leu 515 520 525 Thr Leu Tyr Ile Gly Asp Ser Val Gly Asp Leu Leu Cys Leu Leu Glu 530 535 540 Ala Asp Val Gly Ile Val Leu Gly Thr Ser Ser Ser Leu Arg Thr Val 545 550 555 560 Gly Asn His Phe Gly Val Ser Phe Val Pro Leu Phe Pro Gly Val Val 565 570 575 Gln Lys Gln Lys Met Cys Thr Gly Val Asp Ser Ser Ser Cys Trp Lys 580 585 590 Gly Leu Ser Gly Val Leu Tyr Thr Ala Ser Ser Trp Ala Glu Ile His 595 600 605 Ala Phe Val Leu Gly Ser 610 <210> 136 <211> 1779 <212> DNA <213> Populus trichocarpa <220> <221> CDS <222> (1)..(1779) <223> Populus trichocarpa gene encoding TMP phosphatase [XP_002325785.2] <400> 136 atg cgc cta ctc ttg ttt act tct cca aac cca atc aaa acc tct tca 48 Met Arg Leu Leu Leu Phe Thr Ser Pro Asn Pro Ile Lys Thr Ser Ser 1 5 10 15 tca cta tat ttc ctc aac tcg ctc cga tcc aac tta acc aaa cgc acc 96 Ser Leu Tyr Phe Leu Asn Ser Leu Arg Ser Asn Leu Thr Lys Arg Thr 20 25 30 ttg cca act cgg aga tct ttc atc cct gca aga atg gca atc cct cca 144 Leu Pro Thr Arg Arg Ser Phe Ile Pro Ala Arg Met Ala Ile Pro Pro 35 40 45 cga tca ata gca tca gcg cca tct tgc act aca aca tca ggc aga agt 192 Arg Ser Ile Ala Ser Ala Pro Ser Cys Thr Thr Thr Ser Gly Arg Ser 50 55 60 aac atc aac att gaa gag ggt ctt gct agt aaa ttc tgg atc aag ttt 240 Asn Ile Asn Ile Glu Glu Gly Leu Ala Ser Lys Phe Trp Ile Lys Phe 65 70 75 80 aga aga gaa tcc gtt ttt gct atg tac act cct ttt gtc atc tct ttg 288 Arg Arg Glu Ser Val Phe Ala Met Tyr Thr Pro Phe Val Ile Ser Leu 85 90 95 gct tct ggc act ctc aag att gat tct ttc agg cat tat atc tct caa 336 Ala Ser Gly Thr Leu Lys Ile Asp Ser Phe Arg His Tyr Ile Ser Gln 100 105 110 gat tct cac ttt ctc aaa tct ttt gct cat gcg ttt gaa tta gcg gaa 384 Asp Ser His Phe Leu Lys Ser Phe Ala His Ala Phe Glu Leu Ala Glu 115 120 125 gag tgt gct gat gat gat gaa gca aag cta gca atc tcc gag ttg agg 432 Glu Cys Ala Asp Asp Asp Glu Ala Lys Leu Ala Ile Ser Glu Leu Arg 130 135 140 aag ggt gtc tta gag gag ctg aag atg cac aat tca ttt gta cag gaa 480 Lys Gly Val Leu Glu Glu Leu Lys Met His Asn Ser Phe Val Gln Glu 145 150 155 160 tgg ggt ata gac cca ggt aaa gag ggg act atc aat tct gct act gta 528 Trp Gly Ile Asp Pro Gly Lys Glu Gly Thr Ile Asn Ser Ala Thr Val 165 170 175 aaa tac aca gat ttc ttg ttg gct aca gct tct ggg aag gtt gaa gga 576 Lys Tyr Thr Asp Phe Leu Leu Ala Thr Ala Ser Gly Lys Val Glu Gly 180 185 190 gtg aaa ggt ctt ggt aaa ctt gca act cct ttt gaa aga aca aaa gtt 624 Val Lys Gly Leu Gly Lys Leu Ala Thr Pro Phe Glu Arg Thr Lys Val 195 200 205 gca gcc tat act ctg ggt gcc atg aca cct tgc atg cgg ctg tat tcc 672 Ala Ala Tyr Thr Leu Gly Ala Met Thr Pro Cys Met Arg Leu Tyr Ser 210 215 220 ttt cta ggc aag gaa ctc cag gca gtt tta gat ccg gag gaa gat ggg 720 Phe Leu Gly Lys Glu Leu Gln Ala Val Leu Asp Pro Glu Glu Asp Gly 225 230 235 240 cac cct tac aag aag tgg att gac agt tat tcg tct gag agt ttt cag 768 His Pro Tyr Lys Lys Trp Ile Asp Ser Tyr Ser Ser Glu Ser Phe Gln 245 250 255 gca tca gct ctg caa act gaa gac ttg ctg gat aaa ctt agt gtc tcc 816 Ala Ser Ala Leu Gln Thr Glu Asp Leu Leu Asp Lys Leu Ser Val Ser 260 265 270 ttg aca ggc gag gag ctt gac atc att gaa aag ctt tat cac cag gcc 864 Leu Thr Gly Glu Glu Leu Asp Ile Ile Glu Lys Leu Tyr His Gln Ala 275 280 285 atg aaa ctt gaa ata gaa ttc ttc ctt gct cag cca att gct cag aca 912 Met Lys Leu Glu Ile Glu Phe Phe Leu Ala Gln Pro Ile Ala Gln Thr 290 295 300 act tta gct ccc ctg aca aaa ggg cat aac cct gaa gaa gac cgg ctt 960 Thr Leu Ala Pro Leu Thr Lys Gly His Asn Pro Glu Glu Asp Arg Leu 305 310 315 320 gtc ata ttt tct gat ttt gat ttg aca tgc act gtt gtt gac tct tct 1008 Val Ile Phe Ser Asp Phe Asp Leu Thr Cys Thr Val Val Asp Ser Ser 325 330 335 gcc att ttg gca gaa att gca ata cta aca gca cca aaa tct gat gtg 1056 Ala Ile Leu Ala Glu Ile Ala Ile Leu Thr Ala Pro Lys Ser Asp Val 340 345 350 gtt caa cct gag act caa att gct cga atg tca tca gct gat ctg agg 1104 Val Gln Pro Glu Thr Gln Ile Ala Arg Met Ser Ser Ala Asp Leu Arg 355 360 365 aac aca tgg ggt ctt ctt tct gga cag tac acg gaa gag tat gaa caa 1152 Asn Thr Trp Gly Leu Leu Ser Gly Gln Tyr Thr Glu Glu Tyr Glu Gln 370 375 380 tgt att gaa agc att atg cca tct gca aaa gtg gaa ttc aac tat gaa 1200 Cys Ile Glu Ser Ile Met Pro Ser Ala Lys Val Glu Phe Asn Tyr Glu 385 390 395 400 gct ctt tgt aaa gca ctt gaa caa ctt tca gac ttt gag cga agg gca 1248 Ala Leu Cys Lys Ala Leu Glu Gln Leu Ser Asp Phe Glu Arg Arg Ala 405 410 415 aat tct aga gtg att gat tct gga gtt ctc aaa ggt ttg aat ctt gaa 1296 Asn Ser Arg Val Ile Asp Ser Gly Val Leu Lys Gly Leu Asn Leu Glu 420 425 430 gat gta aaa cga gcg ggt gaa cgt ttg att ctt cag gat ggt tgc att 1344 Asp Val Lys Arg Ala Gly Glu Arg Leu Ile Leu Gln Asp Gly Cys Ile 435 440 445 ggt ttc ttt cag aaa att gtg aag aat gaa aat ttg aac act aat gtc 1392 Gly Phe Phe Gln Lys Ile Val Lys Asn Glu Asn Leu Asn Thr Asn Val 450 455 460 cat gtg ctc tca tac tgc tgg tgt ggt gat ctc atc aga tca gct ttc 1440 His Val Leu Ser Tyr Cys Trp Cys Gly Asp Leu Ile Arg Ser Ala Phe 465 470 475 480 tcc tca ggg ggt ttg gat gct cta aat att cat gca aat gag tta att 1488 Ser Ser Gly Gly Leu Asp Ala Leu Asn Ile His Ala Asn Glu Leu Ile 485 490 495 ttt gaa gaa tca atc tcc acg gga gag att aac ttg act gtt tac att 1536 Phe Glu Glu Ser Ile Ser Thr Gly Glu Ile Asn Leu Thr Val Tyr Ile 500 505 510 gga gat tca gtt ggt gac ttg ctt tgt cta ctt cag gca gat att ggt 1584 Gly Asp Ser Val Gly Asp Leu Leu Cys Leu Leu Gln Ala Asp Ile Gly 515 520 525 att gta gtt gga tct agt gca agc tta agg agc gtg gga agt caa tat 1632 Ile Val Val Gly Ser Ser Ala Ser Leu Arg Ser Val Gly Ser Gln Tyr 530 535 540 ggt gtt tct ttt gta cca ctg ttc cct ggc ttg gta aga aaa cag aaa 1680 Gly Val Ser Phe Val Pro Leu Phe Pro Gly Leu Val Arg Lys Gln Lys 545 550 555 560 gaa tct gat gga gaa tct cct aat tgg aaa ggg cta tct ggc ata cta 1728 Glu Ser Asp Gly Glu Ser Pro Asn Trp Lys Gly Leu Ser Gly Ile Leu 565 570 575 tat aca gtc tcc agt tgg tca gaa ata cat gcc ttc att ttg ggg tgg 1776 Tyr Thr Val Ser Ser Trp Ser Glu Ile His Ala Phe Ile Leu Gly Trp 580 585 590 tag 1779 <210> 137 <211> 592 <212> PRT <213> Populus trichocarpa <400> 137 Met Arg Leu Leu Leu Phe Thr Ser Pro Asn Pro Ile Lys Thr Ser Ser 1 5 10 15 Ser Leu Tyr Phe Leu Asn Ser Leu Arg Ser Asn Leu Thr Lys Arg Thr 20 25 30 Leu Pro Thr Arg Arg Ser Phe Ile Pro Ala Arg Met Ala Ile Pro Pro 35 40 45 Arg Ser Ile Ala Ser Ala Pro Ser Cys Thr Thr Thr Ser Gly Arg Ser 50 55 60 Asn Ile Asn Ile Glu Glu Gly Leu Ala Ser Lys Phe Trp Ile Lys Phe 65 70 75 80 Arg Arg Glu Ser Val Phe Ala Met Tyr Thr Pro Phe Val Ile Ser Leu 85 90 95 Ala Ser Gly Thr Leu Lys Ile Asp Ser Phe Arg His Tyr Ile Ser Gln 100 105 110 Asp Ser His Phe Leu Lys Ser Phe Ala His Ala Phe Glu Leu Ala Glu 115 120 125 Glu Cys Ala Asp Asp Asp Glu Ala Lys Leu Ala Ile Ser Glu Leu Arg 130 135 140 Lys Gly Val Leu Glu Glu Leu Lys Met His Asn Ser Phe Val Gln Glu 145 150 155 160 Trp Gly Ile Asp Pro Gly Lys Glu Gly Thr Ile Asn Ser Ala Thr Val 165 170 175 Lys Tyr Thr Asp Phe Leu Leu Ala Thr Ala Ser Gly Lys Val Glu Gly 180 185 190 Val Lys Gly Leu Gly Lys Leu Ala Thr Pro Phe Glu Arg Thr Lys Val 195 200 205 Ala Ala Tyr Thr Leu Gly Ala Met Thr Pro Cys Met Arg Leu Tyr Ser 210 215 220 Phe Leu Gly Lys Glu Leu Gln Ala Val Leu Asp Pro Glu Glu Asp Gly 225 230 235 240 His Pro Tyr Lys Lys Trp Ile Asp Ser Tyr Ser Ser Glu Ser Phe Gln 245 250 255 Ala Ser Ala Leu Gln Thr Glu Asp Leu Leu Asp Lys Leu Ser Val Ser 260 265 270 Leu Thr Gly Glu Glu Leu Asp Ile Ile Glu Lys Leu Tyr His Gln Ala 275 280 285 Met Lys Leu Glu Ile Glu Phe Phe Leu Ala Gln Pro Ile Ala Gln Thr 290 295 300 Thr Leu Ala Pro Leu Thr Lys Gly His Asn Pro Glu Glu Asp Arg Leu 305 310 315 320 Val Ile Phe Ser Asp Phe Asp Leu Thr Cys Thr Val Val Asp Ser Ser 325 330 335 Ala Ile Leu Ala Glu Ile Ala Ile Leu Thr Ala Pro Lys Ser Asp Val 340 345 350 Val Gln Pro Glu Thr Gln Ile Ala Arg Met Ser Ser Ala Asp Leu Arg 355 360 365 Asn Thr Trp Gly Leu Leu Ser Gly Gln Tyr Thr Glu Glu Tyr Glu Gln 370 375 380 Cys Ile Glu Ser Ile Met Pro Ser Ala Lys Val Glu Phe Asn Tyr Glu 385 390 395 400 Ala Leu Cys Lys Ala Leu Glu Gln Leu Ser Asp Phe Glu Arg Arg Ala 405 410 415 Asn Ser Arg Val Ile Asp Ser Gly Val Leu Lys Gly Leu Asn Leu Glu 420 425 430 Asp Val Lys Arg Ala Gly Glu Arg Leu Ile Leu Gln Asp Gly Cys Ile 435 440 445 Gly Phe Phe Gln Lys Ile Val Lys Asn Glu Asn Leu Asn Thr Asn Val 450 455 460 His Val Leu Ser Tyr Cys Trp Cys Gly Asp Leu Ile Arg Ser Ala Phe 465 470 475 480 Ser Ser Gly Gly Leu Asp Ala Leu Asn Ile His Ala Asn Glu Leu Ile 485 490 495 Phe Glu Glu Ser Ile Ser Thr Gly Glu Ile Asn Leu Thr Val Tyr Ile 500 505 510 Gly Asp Ser Val Gly Asp Leu Leu Cys Leu Leu Gln Ala Asp Ile Gly 515 520 525 Ile Val Val Gly Ser Ser Ala Ser Leu Arg Ser Val Gly Ser Gln Tyr 530 535 540 Gly Val Ser Phe Val Pro Leu Phe Pro Gly Leu Val Arg Lys Gln Lys 545 550 555 560 Glu Ser Asp Gly Glu Ser Pro Asn Trp Lys Gly Leu Ser Gly Ile Leu 565 570 575 Tyr Thr Val Ser Ser Trp Ser Glu Ile His Ala Phe Ile Leu Gly Trp 580 585 590 <210> 138 <211> 1860 <212> DNA <213> Jatropha curcas <220> <221> CDS <222> (1)..(1860) <223> Jatropha curcas gene encoding TMP phosphatase [KDP23738.1] <400> 138 atg gcg atc cct cca aag cta gct tcc tca tcg tct tcc atg gcc gcc 48 Met Ala Ile Pro Pro Lys Leu Ala Ser Ser Ser Ser Ser Met Ala Ala 1 5 10 15 tcc cct act tct gct ggt gga acc aac gag gaa ggc ctc gct agt aaa 96 Ser Pro Thr Ser Ala Gly Gly Thr Asn Glu Glu Gly Leu Ala Ser Lys 20 25 30 ttc tgg atc aag ttt cgc cga gaa tcg gtt ctc gct atg tac act cct 144 Phe Trp Ile Lys Phe Arg Arg Glu Ser Val Leu Ala Met Tyr Thr Pro 35 40 45 ttc gtc gtc tct ttt gcc gcc ggc aac ctc aag att gag agt ttt agg 192 Phe Val Val Ser Phe Ala Ala Gly Asn Leu Lys Ile Glu Ser Phe Arg 50 55 60 cat tac atc gct cag gat ttt cac ttc ctc aaa gcc ttc gct cac gcg 240 His Tyr Ile Ala Gln Asp Phe His Phe Leu Lys Ala Phe Ala His Ala 65 70 75 80 tat gaa ttg gca gaa gag tgt gct gat gat gat gat gcc aag cta gct 288 Tyr Glu Leu Ala Glu Glu Cys Ala Asp Asp Asp Asp Ala Lys Leu Ala 85 90 95 att gcc gcg ttg agg aag ggg gtc tta gag gag ctg aag ttg cat aaa 336 Ile Ala Ala Leu Arg Lys Gly Val Leu Glu Glu Leu Lys Leu His Lys 100 105 110 tca ttt gta cag gaa tgg ggt atg gac cct tcc aaa gag gtg act atc 384 Ser Phe Val Gln Glu Trp Gly Met Asp Pro Ser Lys Glu Val Thr Ile 115 120 125 aat tct gca act gca aaa tac aca gat ttc ttg ttg gct aca gct tct 432 Asn Ser Ala Thr Ala Lys Tyr Thr Asp Phe Leu Leu Ala Thr Ala Ser 130 135 140 gga aag gtt gaa gga gtg aaa ggt cct ggt aaa ctt gca act cct ttt 480 Gly Lys Val Glu Gly Val Lys Gly Pro Gly Lys Leu Ala Thr Pro Phe 145 150 155 160 gaa aga aca aaa gtt gca gct tac act ctt ggt acc atg aca ccc tgt 528 Glu Arg Thr Lys Val Ala Ala Tyr Thr Leu Gly Thr Met Thr Pro Cys 165 170 175 atg agg ttg tat gcc ttt cta gct aag gag ctg caa gca cta ata gat 576 Met Arg Leu Tyr Ala Phe Leu Ala Lys Glu Leu Gln Ala Leu Ile Asp 180 185 190 gca gaa gct ggt att cat cct tac cag aag tgg att gac aat tac tca 624 Ala Glu Ala Gly Ile His Pro Tyr Gln Lys Trp Ile Asp Asn Tyr Ser 195 200 205 tct gag agt ttt cag gca tca gct ctg caa act gaa gac ttg ctg gat 672 Ser Glu Ser Phe Gln Ala Ser Ala Leu Gln Thr Glu Asp Leu Leu Asp 210 215 220 aaa ctt agt gtc cct ttg aca ggc gaa gag ctt gac atc att gaa aag 720 Lys Leu Ser Val Pro Leu Thr Gly Glu Glu Leu Asp Ile Ile Glu Lys 225 230 235 240 ctt tat cac caa gcc atg aaa ctt gaa ata gag ttc ttc aat gcg cag 768 Leu Tyr His Gln Ala Met Lys Leu Glu Ile Glu Phe Phe Asn Ala Gln 245 250 255 cca ctt gat cag ccc act gtg gtt cct ctg aca aaa gag cat aac cct 816 Pro Leu Asp Gln Pro Thr Val Val Pro Leu Thr Lys Glu His Asn Pro 260 265 270 cta gaa gat cgc ctc gtg ata ttt tct gat ttt gat ttg aca tgc aca 864 Leu Glu Asp Arg Leu Val Ile Phe Ser Asp Phe Asp Leu Thr Cys Thr 275 280 285 gtt gtt gat tcc tct gcc att ttg gca gag att gca att tta aca gca 912 Val Val Asp Ser Ser Ala Ile Leu Ala Glu Ile Ala Ile Leu Thr Ala 290 295 300 tca aaa tct gat cag tca caa tct gat aat caa aat gct agg atg tca 960 Ser Lys Ser Asp Gln Ser Gln Ser Asp Asn Gln Asn Ala Arg Met Ser 305 310 315 320 tca act gag cta agg aac aca tgg gtt ctt ctc tct gga cag tat act 1008 Ser Thr Glu Leu Arg Asn Thr Trp Val Leu Leu Ser Gly Gln Tyr Thr 325 330 335 gaa gaa tat gag caa tgc att gaa agc att ctg ccc tct gaa aaa atg 1056 Glu Glu Tyr Glu Gln Cys Ile Glu Ser Ile Leu Pro Ser Glu Lys Met 340 345 350 gag ttc aac ttt gaa gct ttg tgt aaa gca ctc gaa caa ctc tca gac 1104 Glu Phe Asn Phe Glu Ala Leu Cys Lys Ala Leu Glu Gln Leu Ser Asp 355 360 365 ttt gag cga agg gca aat gct aga gtt atc aaa tct gga gtt ctt aag 1152 Phe Glu Arg Arg Ala Asn Ala Arg Val Ile Lys Ser Gly Val Leu Lys 370 375 380 ggt ttg aat ctt gaa gac ata aaa cga gct gtg gag ttc aac ttt gaa 1200 Gly Leu Asn Leu Glu Asp Ile Lys Arg Ala Val Glu Phe Asn Phe Glu 385 390 395 400 gct ttg tgt aaa gca ctc gaa caa ctc tca gac ttt gag cga agg gca 1248 Ala Leu Cys Lys Ala Leu Glu Gln Leu Ser Asp Phe Glu Arg Arg Ala 405 410 415 aat gct aga gtt atc aaa tct gga gtt ctt aag ggt ttg aat ctt gaa 1296 Asn Ala Arg Val Ile Lys Ser Gly Val Leu Lys Gly Leu Asn Leu Glu 420 425 430 gac ata aaa cga gct ggt gaa aga ctg att ctt caa gat ggc tgc acc 1344 Asp Ile Lys Arg Ala Gly Glu Arg Leu Ile Leu Gln Asp Gly Cys Thr 435 440 445 agt ttt ttt cag aaa atc tcg aag aat gaa aat ctg aat gct aat ata 1392 Ser Phe Phe Gln Lys Ile Ser Lys Asn Glu Asn Leu Asn Ala Asn Ile 450 455 460 cat ttc ctc tca tat tgt tgg tgt gct gat ctg atc aga tct gct ttc 1440 His Phe Leu Ser Tyr Cys Trp Cys Ala Asp Leu Ile Arg Ser Ala Phe 465 470 475 480 tca tca ggg ggt ttg gat gtt ctg aat ata cat gcg aat gag ttt gat 1488 Ser Ser Gly Gly Leu Asp Val Leu Asn Ile His Ala Asn Glu Phe Asp 485 490 495 ttc gta gaa tca att tca acg ggt gag att att atg aag gtg gaa acc 1536 Phe Val Glu Ser Ile Ser Thr Gly Glu Ile Ile Met Lys Val Glu Thr 500 505 510 cct aca gac aaa gcc caa gct ttt aat aat att tta atg aac tac agc 1584 Pro Thr Asp Lys Ala Gln Ala Phe Asn Asn Ile Leu Met Asn Tyr Ser 515 520 525 cct gac aaa aag aat ttg act gtt tat att gga gac tca gtt ggg gac 1632 Pro Asp Lys Lys Asn Leu Thr Val Tyr Ile Gly Asp Ser Val Gly Asp 530 535 540 ttg ctt tgt ctg ctt gcg gca gat ata ggc atc gtg atc gga tca agc 1680 Leu Leu Cys Leu Leu Ala Ala Asp Ile Gly Ile Val Ile Gly Ser Ser 545 550 555 560 tcc agc cta agg aga gtc gga agt cag ttt ggt gta aca ttt tta cca 1728 Ser Ser Leu Arg Arg Val Gly Ser Gln Phe Gly Val Thr Phe Leu Pro 565 570 575 ttg tat cct ggc ttg gtt aaa aaa cag aga gag tat act gaa gga agc 1776 Leu Tyr Pro Gly Leu Val Lys Lys Gln Arg Glu Tyr Thr Glu Gly Ser 580 585 590 tct tgg aat tgg aag ggt caa tct ggc gtt ctg tac aca gtt tct agt 1824 Ser Trp Asn Trp Lys Gly Gln Ser Gly Val Leu Tyr Thr Val Ser Ser 595 600 605 tgg gct gaa ata cat tcc ttc gtt ttg gga tgg tag 1860 Trp Ala Glu Ile His Ser Phe Val Leu Gly Trp 610 615 <210> 139 <211> 619 <212> PRT <213> Jatropha curcas <400> 139 Met Ala Ile Pro Pro Lys Leu Ala Ser Ser Ser Ser Ser Met Ala Ala 1 5 10 15 Ser Pro Thr Ser Ala Gly Gly Thr Asn Glu Glu Gly Leu Ala Ser Lys 20 25 30 Phe Trp Ile Lys Phe Arg Arg Glu Ser Val Leu Ala Met Tyr Thr Pro 35 40 45 Phe Val Val Ser Phe Ala Ala Gly Asn Leu Lys Ile Glu Ser Phe Arg 50 55 60 His Tyr Ile Ala Gln Asp Phe His Phe Leu Lys Ala Phe Ala His Ala 65 70 75 80 Tyr Glu Leu Ala Glu Glu Cys Ala Asp Asp Asp Asp Ala Lys Leu Ala 85 90 95 Ile Ala Ala Leu Arg Lys Gly Val Leu Glu Glu Leu Lys Leu His Lys 100 105 110 Ser Phe Val Gln Glu Trp Gly Met Asp Pro Ser Lys Glu Val Thr Ile 115 120 125 Asn Ser Ala Thr Ala Lys Tyr Thr Asp Phe Leu Leu Ala Thr Ala Ser 130 135 140 Gly Lys Val Glu Gly Val Lys Gly Pro Gly Lys Leu Ala Thr Pro Phe 145 150 155 160 Glu Arg Thr Lys Val Ala Ala Tyr Thr Leu Gly Thr Met Thr Pro Cys 165 170 175 Met Arg Leu Tyr Ala Phe Leu Ala Lys Glu Leu Gln Ala Leu Ile Asp 180 185 190 Ala Glu Ala Gly Ile His Pro Tyr Gln Lys Trp Ile Asp Asn Tyr Ser 195 200 205 Ser Glu Ser Phe Gln Ala Ser Ala Leu Gln Thr Glu Asp Leu Leu Asp 210 215 220 Lys Leu Ser Val Pro Leu Thr Gly Glu Glu Leu Asp Ile Ile Glu Lys 225 230 235 240 Leu Tyr His Gln Ala Met Lys Leu Glu Ile Glu Phe Phe Asn Ala Gln 245 250 255 Pro Leu Asp Gln Pro Thr Val Val Pro Leu Thr Lys Glu His Asn Pro 260 265 270 Leu Glu Asp Arg Leu Val Ile Phe Ser Asp Phe Asp Leu Thr Cys Thr 275 280 285 Val Val Asp Ser Ser Ala Ile Leu Ala Glu Ile Ala Ile Leu Thr Ala 290 295 300 Ser Lys Ser Asp Gln Ser Gln Ser Asp Asn Gln Asn Ala Arg Met Ser 305 310 315 320 Ser Thr Glu Leu Arg Asn Thr Trp Val Leu Leu Ser Gly Gln Tyr Thr 325 330 335 Glu Glu Tyr Glu Gln Cys Ile Glu Ser Ile Leu Pro Ser Glu Lys Met 340 345 350 Glu Phe Asn Phe Glu Ala Leu Cys Lys Ala Leu Glu Gln Leu Ser Asp 355 360 365 Phe Glu Arg Arg Ala Asn Ala Arg Val Ile Lys Ser Gly Val Leu Lys 370 375 380 Gly Leu Asn Leu Glu Asp Ile Lys Arg Ala Val Glu Phe Asn Phe Glu 385 390 395 400 Ala Leu Cys Lys Ala Leu Glu Gln Leu Ser Asp Phe Glu Arg Arg Ala 405 410 415 Asn Ala Arg Val Ile Lys Ser Gly Val Leu Lys Gly Leu Asn Leu Glu 420 425 430 Asp Ile Lys Arg Ala Gly Glu Arg Leu Ile Leu Gln Asp Gly Cys Thr 435 440 445 Ser Phe Phe Gln Lys Ile Ser Lys Asn Glu Asn Leu Asn Ala Asn Ile 450 455 460 His Phe Leu Ser Tyr Cys Trp Cys Ala Asp Leu Ile Arg Ser Ala Phe 465 470 475 480 Ser Ser Gly Gly Leu Asp Val Leu Asn Ile His Ala Asn Glu Phe Asp 485 490 495 Phe Val Glu Ser Ile Ser Thr Gly Glu Ile Ile Met Lys Val Glu Thr 500 505 510 Pro Thr Asp Lys Ala Gln Ala Phe Asn Asn Ile Leu Met Asn Tyr Ser 515 520 525 Pro Asp Lys Lys Asn Leu Thr Val Tyr Ile Gly Asp Ser Val Gly Asp 530 535 540 Leu Leu Cys Leu Leu Ala Ala Asp Ile Gly Ile Val Ile Gly Ser Ser 545 550 555 560 Ser Ser Leu Arg Arg Val Gly Ser Gln Phe Gly Val Thr Phe Leu Pro 565 570 575 Leu Tyr Pro Gly Leu Val Lys Lys Gln Arg Glu Tyr Thr Glu Gly Ser 580 585 590 Ser Trp Asn Trp Lys Gly Gln Ser Gly Val Leu Tyr Thr Val Ser Ser 595 600 605 Trp Ala Glu Ile His Ser Phe Val Leu Gly Trp 610 615 <210> 140 <211> 1842 <212> DNA <213> Citrus sinensis <220> <221> CDS <222> (1)..(1842) <223> Citrus sinensi gene encoding TMP phosphatase [XP_006484613.1] <400> 140 atg cgc ttc ctt ttc aca aac cca atc aaa acc cca tta ctc tct tct 48 Met Arg Phe Leu Phe Thr Asn Pro Ile Lys Thr Pro Leu Leu Ser Ser 1 5 10 15 att ctt ttc cat tgt ccc aac tcg ccc cga ctc ggc ctt ctt gac tca 96 Ile Leu Phe His Cys Pro Asn Ser Pro Arg Leu Gly Leu Leu Asp Ser 20 25 30 gtc cga gtc aac tca cct tct tct ttg aca act caa aga tcg tca ctt 144 Val Arg Val Asn Ser Pro Ser Ser Leu Thr Thr Gln Arg Ser Ser Leu 35 40 45 tcg atg gcg gcg att ccc cca aaa tcg ccg agc cct gag gag gag gga 192 Ser Met Ala Ala Ile Pro Pro Lys Ser Pro Ser Pro Glu Glu Glu Gly 50 55 60 ctc gcg agg agg ttg tgg atc aag ttt aag aga gaa tct gtg ttt gcc 240 Leu Ala Arg Arg Leu Trp Ile Lys Phe Lys Arg Glu Ser Val Phe Ala 65 70 75 80 atg tac tcc ccg ttt acg gtt tgt ttg gct tct ggg aac cta aag ctt 288 Met Tyr Ser Pro Phe Thr Val Cys Leu Ala Ser Gly Asn Leu Lys Leu 85 90 95 gaa acc ttc agg cat tac atc gcc caa gat ttt cat ttt ctc aaa gct 336 Glu Thr Phe Arg His Tyr Ile Ala Gln Asp Phe His Phe Leu Lys Ala 100 105 110 ttc gcc caa gcg tat gaa ctg gcg gaa gaa tgt gct gat gat gat gat 384 Phe Ala Gln Ala Tyr Glu Leu Ala Glu Glu Cys Ala Asp Asp Asp Asp 115 120 125 gca aag tta tct atc tct gaa ttg agg aag ggt gta ctt gag gag tta 432 Ala Lys Leu Ser Ile Ser Glu Leu Arg Lys Gly Val Leu Glu Glu Leu 130 135 140 aaa atg cat gat tcc ttt gtg aag gag tgg ggt aca gat ctt gct aaa 480 Lys Met His Asp Ser Phe Val Lys Glu Trp Gly Thr Asp Leu Ala Lys 145 150 155 160 atg gct act gtt aac tct gca act gta aag tat aca gag ttc ttg ttg 528 Met Ala Thr Val Asn Ser Ala Thr Val Lys Tyr Thr Glu Phe Leu Leu 165 170 175 gca aca gct tcc ggg aag gtc gaa ggt gtt aaa ggt cct gga aaa ctt 576 Ala Thr Ala Ser Gly Lys Val Glu Gly Val Lys Gly Pro Gly Lys Leu 180 185 190 gca acc cca ttt gag aaa act aaa gtt gcc gct tac aca ttg ggt gcc 624 Ala Thr Pro Phe Glu Lys Thr Lys Val Ala Ala Tyr Thr Leu Gly Ala 195 200 205 atg tca cct tgt atg agg ctc tat gct ttc ctt gga aag gaa ttc cat 672 Met Ser Pro Cys Met Arg Leu Tyr Ala Phe Leu Gly Lys Glu Phe His 210 215 220 ggc ctc cta aat gct aat gaa ggc aat cat cct tac aag aag tgg att 720 Gly Leu Leu Asn Ala Asn Glu Gly Asn His Pro Tyr Lys Lys Trp Ile 225 230 235 240 gac aat tat tct tct gaa agt ttt cag gcc tca gct ctg caa aat gag 768 Asp Asn Tyr Ser Ser Glu Ser Phe Gln Ala Ser Ala Leu Gln Asn Glu 245 250 255 gac ttg ctg gat aaa ctt agt gtc tct ttg aca ggc gaa gaa cta gac 816 Asp Leu Leu Asp Lys Leu Ser Val Ser Leu Thr Gly Glu Glu Leu Asp 260 265 270 ata ata gaa aag ctc tat cac caa gcc atg aaa ctt gaa gta gag ttc 864 Ile Ile Glu Lys Leu Tyr His Gln Ala Met Lys Leu Glu Val Glu Phe 275 280 285 ttc tgt gct cag cca ctt gct cag ccc act gta gtt cct ctg att aaa 912 Phe Cys Ala Gln Pro Leu Ala Gln Pro Thr Val Val Pro Leu Ile Lys 290 295 300 ggg cat aat cct gca gga gac cgt cta att ata ttt tct gat ttc gat 960 Gly His Asn Pro Ala Gly Asp Arg Leu Ile Ile Phe Ser Asp Phe Asp 305 310 315 320 ttg act tgc acc att gtt gat tcc tct gcc att ttg gca gag atc gca 1008 Leu Thr Cys Thr Ile Val Asp Ser Ser Ala Ile Leu Ala Glu Ile Ala 325 330 335 ata gtg aca gca cca aaa tct gac cag aat caa cct gaa aat caa ctt 1056 Ile Val Thr Ala Pro Lys Ser Asp Gln Asn Gln Pro Glu Asn Gln Leu 340 345 350 ggt cgg atg tca tca ggt gag ctg agg aac aca tgg ggt ctt ctt tcc 1104 Gly Arg Met Ser Ser Gly Glu Leu Arg Asn Thr Trp Gly Leu Leu Ser 355 360 365 aaa cag tac aca gag gag tac gaa caa tgc att gaa agc ttc atg ccc 1152 Lys Gln Tyr Thr Glu Glu Tyr Glu Gln Cys Ile Glu Ser Phe Met Pro 370 375 380 tct gag aaa gtg gag aat ttc aac tat gaa act ttg cat aaa gca ctt 1200 Ser Glu Lys Val Glu Asn Phe Asn Tyr Glu Thr Leu His Lys Ala Leu 385 390 395 400 gag caa ctc tca cac ttt gag aag agg gca aat tct aga gtg atc gaa 1248 Glu Gln Leu Ser His Phe Glu Lys Arg Ala Asn Ser Arg Val Ile Glu 405 410 415 tct gga gtt ctc aag ggt ata aat ctt gaa gat att aaa aaa gct ggt 1296 Ser Gly Val Leu Lys Gly Ile Asn Leu Glu Asp Ile Lys Lys Ala Gly 420 425 430 gaa cgc ctg agt ctt caa gat ggt tgc act acc ttc ttt cag aaa gtt 1344 Glu Arg Leu Ser Leu Gln Asp Gly Cys Thr Thr Phe Phe Gln Lys Val 435 440 445 gta aag aat gaa aat ttg aat gct aat gtc cat gtg ctt tca tac tgt 1392 Val Lys Asn Glu Asn Leu Asn Ala Asn Val His Val Leu Ser Tyr Cys 450 455 460 tgg tgt ggt gat ctc atc aga gca tct ttt tct tca gca ggt tta aat 1440 Trp Cys Gly Asp Leu Ile Arg Ala Ser Phe Ser Ser Ala Gly Leu Asn 465 470 475 480 gca ctg aat gta cat gcg aat gag ttc tca ttc aaa gaa tct att tca 1488 Ala Leu Asn Val His Ala Asn Glu Phe Ser Phe Lys Glu Ser Ile Ser 485 490 495 acg ggt gaa att att gag aaa gtg gag tcc ccc att gac aaa gtt caa 1536 Thr Gly Glu Ile Ile Glu Lys Val Glu Ser Pro Ile Asp Lys Val Gln 500 505 510 gct ttc aac aat act tta gag aaa tac gga act gac aga aag aac ttg 1584 Ala Phe Asn Asn Thr Leu Glu Lys Tyr Gly Thr Asp Arg Lys Asn Leu 515 520 525 agt gtt tac att gga gac tct gtg ggt gac ttg ctt tgt ctg ctt gag 1632 Ser Val Tyr Ile Gly Asp Ser Val Gly Asp Leu Leu Cys Leu Leu Glu 530 535 540 gct gat ata ggc att gta atc ggg tct agc tca agc tta agg aga gtg 1680 Ala Asp Ile Gly Ile Val Ile Gly Ser Ser Ser Ser Leu Arg Arg Val 545 550 555 560 gga tct caa ttt ggt gtt aca ttt atc ccg ttg tac cct ggc ttg gtt 1728 Gly Ser Gln Phe Gly Val Thr Phe Ile Pro Leu Tyr Pro Gly Leu Val 565 570 575 aag aaa cag aag gag tac act gaa gga agc tct tct aac tgg aag gag 1776 Lys Lys Gln Lys Glu Tyr Thr Glu Gly Ser Ser Ser Asn Trp Lys Glu 580 585 590 aaa tct ggc ata ctt tac aca gtc tca agt tgg gct gaa gta cat gcc 1824 Lys Ser Gly Ile Leu Tyr Thr Val Ser Ser Trp Ala Glu Val His Ala 595 600 605 ttt atc ttg ggg tgg tag 1842 Phe Ile Leu Gly Trp 610 <210> 141 <211> 613 <212> PRT <213> Citrus sinensis <400> 141 Met Arg Phe Leu Phe Thr Asn Pro Ile Lys Thr Pro Leu Leu Ser Ser 1 5 10 15 Ile Leu Phe His Cys Pro Asn Ser Pro Arg Leu Gly Leu Leu Asp Ser 20 25 30 Val Arg Val Asn Ser Pro Ser Ser Leu Thr Thr Gln Arg Ser Ser Leu 35 40 45 Ser Met Ala Ala Ile Pro Pro Lys Ser Pro Ser Pro Glu Glu Glu Gly 50 55 60 Leu Ala Arg Arg Leu Trp Ile Lys Phe Lys Arg Glu Ser Val Phe Ala 65 70 75 80 Met Tyr Ser Pro Phe Thr Val Cys Leu Ala Ser Gly Asn Leu Lys Leu 85 90 95 Glu Thr Phe Arg His Tyr Ile Ala Gln Asp Phe His Phe Leu Lys Ala 100 105 110 Phe Ala Gln Ala Tyr Glu Leu Ala Glu Glu Cys Ala Asp Asp Asp Asp 115 120 125 Ala Lys Leu Ser Ile Ser Glu Leu Arg Lys Gly Val Leu Glu Glu Leu 130 135 140 Lys Met His Asp Ser Phe Val Lys Glu Trp Gly Thr Asp Leu Ala Lys 145 150 155 160 Met Ala Thr Val Asn Ser Ala Thr Val Lys Tyr Thr Glu Phe Leu Leu 165 170 175 Ala Thr Ala Ser Gly Lys Val Glu Gly Val Lys Gly Pro Gly Lys Leu 180 185 190 Ala Thr Pro Phe Glu Lys Thr Lys Val Ala Ala Tyr Thr Leu Gly Ala 195 200 205 Met Ser Pro Cys Met Arg Leu Tyr Ala Phe Leu Gly Lys Glu Phe His 210 215 220 Gly Leu Leu Asn Ala Asn Glu Gly Asn His Pro Tyr Lys Lys Trp Ile 225 230 235 240 Asp Asn Tyr Ser Ser Glu Ser Phe Gln Ala Ser Ala Leu Gln Asn Glu 245 250 255 Asp Leu Leu Asp Lys Leu Ser Val Ser Leu Thr Gly Glu Glu Leu Asp 260 265 270 Ile Ile Glu Lys Leu Tyr His Gln Ala Met Lys Leu Glu Val Glu Phe 275 280 285 Phe Cys Ala Gln Pro Leu Ala Gln Pro Thr Val Val Pro Leu Ile Lys 290 295 300 Gly His Asn Pro Ala Gly Asp Arg Leu Ile Ile Phe Ser Asp Phe Asp 305 310 315 320 Leu Thr Cys Thr Ile Val Asp Ser Ser Ala Ile Leu Ala Glu Ile Ala 325 330 335 Ile Val Thr Ala Pro Lys Ser Asp Gln Asn Gln Pro Glu Asn Gln Leu 340 345 350 Gly Arg Met Ser Ser Gly Glu Leu Arg Asn Thr Trp Gly Leu Leu Ser 355 360 365 Lys Gln Tyr Thr Glu Glu Tyr Glu Gln Cys Ile Glu Ser Phe Met Pro 370 375 380 Ser Glu Lys Val Glu Asn Phe Asn Tyr Glu Thr Leu His Lys Ala Leu 385 390 395 400 Glu Gln Leu Ser His Phe Glu Lys Arg Ala Asn Ser Arg Val Ile Glu 405 410 415 Ser Gly Val Leu Lys Gly Ile Asn Leu Glu Asp Ile Lys Lys Ala Gly 420 425 430 Glu Arg Leu Ser Leu Gln Asp Gly Cys Thr Thr Phe Phe Gln Lys Val 435 440 445 Val Lys Asn Glu Asn Leu Asn Ala Asn Val His Val Leu Ser Tyr Cys 450 455 460 Trp Cys Gly Asp Leu Ile Arg Ala Ser Phe Ser Ser Ala Gly Leu Asn 465 470 475 480 Ala Leu Asn Val His Ala Asn Glu Phe Ser Phe Lys Glu Ser Ile Ser 485 490 495 Thr Gly Glu Ile Ile Glu Lys Val Glu Ser Pro Ile Asp Lys Val Gln 500 505 510 Ala Phe Asn Asn Thr Leu Glu Lys Tyr Gly Thr Asp Arg Lys Asn Leu 515 520 525 Ser Val Tyr Ile Gly Asp Ser Val Gly Asp Leu Leu Cys Leu Leu Glu 530 535 540 Ala Asp Ile Gly Ile Val Ile Gly Ser Ser Ser Ser Leu Arg Arg Val 545 550 555 560 Gly Ser Gln Phe Gly Val Thr Phe Ile Pro Leu Tyr Pro Gly Leu Val 565 570 575 Lys Lys Gln Lys Glu Tyr Thr Glu Gly Ser Ser Ser Asn Trp Lys Glu 580 585 590 Lys Ser Gly Ile Leu Tyr Thr Val Ser Ser Trp Ala Glu Val His Ala 595 600 605 Phe Ile Leu Gly Trp 610 <210> 142 <211> 1728 <212> DNA <213> Prunus persica <220> <221> CDS <222> (1)..(1728) <223> Prunus persica gene encoding TMP phosphatase [XP_007199656.1] <400> 142 atg gcg gca ttg gct cgt cat agc att gtt aga ctc aat cac gaa gga 48 Met Ala Ala Leu Ala Arg His Ser Ile Val Arg Leu Asn His Glu Gly 1 5 10 15 ggc cta gcc aga cgg ctg tgg ttc aag ttc aga gac gac tct gtt ttc 96 Gly Leu Ala Arg Arg Leu Trp Phe Lys Phe Arg Asp Asp Ser Val Phe 20 25 30 tct ctc tac act ccc ttc ttc gtt ggc tta gcc tct gct act ctg cac 144 Ser Leu Tyr Thr Pro Phe Phe Val Gly Leu Ala Ser Ala Thr Leu His 35 40 45 tct gaa act acc ttt cgc cat ttc atc tct cag gac ctc cat ttt ctc 192 Ser Glu Thr Thr Phe Arg His Phe Ile Ser Gln Asp Leu His Phe Leu 50 55 60 aaa gcc ttc gtt ctc gca tat gaa ttg gcg gaa gat tgt gct gat gat 240 Lys Ala Phe Val Leu Ala Tyr Glu Leu Ala Glu Asp Cys Ala Asp Asp 65 70 75 80 gag gac gac aag aat ggt tta cgc gat ttg aga aaa cgt gcc gtc ggc 288 Glu Asp Asp Lys Asn Gly Leu Arg Asp Leu Arg Lys Arg Ala Val Gly 85 90 95 agg ctt caa atg cac gac aca ttt gtc cga gaa tgg ggt ttt gaa ttc 336 Arg Leu Gln Met His Asp Thr Phe Val Arg Glu Trp Gly Phe Glu Phe 100 105 110 cca aat gag gac att tct aaa gac att gca aca acc aaa tac aca gat 384 Pro Asn Glu Asp Ile Ser Lys Asp Ile Ala Thr Thr Lys Tyr Thr Asp 115 120 125 ttc ttg ctt gca aca gca tca ggg aaa att gaa gga gaa aga tcg gtt 432 Phe Leu Leu Ala Thr Ala Ser Gly Lys Ile Glu Gly Glu Arg Ser Val 130 135 140 ctg gac aaa atc gca acc cct ttc gaa aag acc aag gtt gct gca tat 480 Leu Asp Lys Ile Ala Thr Pro Phe Glu Lys Thr Lys Val Ala Ala Tyr 145 150 155 160 aca ctt gct gct ctg gct cct tgt atg aga ctc tat gcc ttc atc agt 528 Thr Leu Ala Ala Leu Ala Pro Cys Met Arg Leu Tyr Ala Phe Ile Ser 165 170 175 act gag atc caa ggc att ata aat cct gat caa gat agc act cac att 576 Thr Glu Ile Gln Gly Ile Ile Asn Pro Asp Gln Asp Ser Thr His Ile 180 185 190 tac aaa agc tgg ata gaa aat tat tcg tct caa gtt ttc gag gaa ata 624 Tyr Lys Ser Trp Ile Glu Asn Tyr Ser Ser Gln Val Phe Glu Glu Ile 195 200 205 gcc ctg caa aat gaa gac atg cta gat aaa ctt agt gtt tct ttg act 672 Ala Leu Gln Asn Glu Asp Met Leu Asp Lys Leu Ser Val Ser Leu Thr 210 215 220 ggt gag gag ctt gag att ata gag aag ctc tat cat caa gct atg aag 720 Gly Glu Glu Leu Glu Ile Ile Glu Lys Leu Tyr His Gln Ala Met Lys 225 230 235 240 ctt caa gta gat ttt att gct gct caa cca att tct gat cag caa tct 768 Leu Gln Val Asp Phe Ile Ala Ala Gln Pro Ile Ser Asp Gln Gln Ser 245 250 255 gta gtt cct ttg tct cgg gtg cat gac ttt agc aaa cgc cat ctt acg 816 Val Val Pro Leu Ser Arg Val His Asp Phe Ser Lys Arg His Leu Thr 260 265 270 ata ctt tgt gac ttt gat ttg gca tgc act gct ttt gat tct gct gcc 864 Ile Leu Cys Asp Phe Asp Leu Ala Cys Thr Ala Phe Asp Ser Ala Ala 275 280 285 ata ttg gct gag att gcg atc ata aca gca cca aag gct gat atg gat 912 Ile Leu Ala Glu Ile Ala Ile Ile Thr Ala Pro Lys Ala Asp Met Asp 290 295 300 gga tct gat caa acc caa ctt gct cgg atg cca tca gca gac tta agg 960 Gly Ser Asp Gln Thr Gln Leu Ala Arg Met Pro Ser Ala Asp Leu Arg 305 310 315 320 agc aca tgg gat gtt ctt tca acc caa tac act gaa caa ttt gaa caa 1008 Ser Thr Trp Asp Val Leu Ser Thr Gln Tyr Thr Glu Gln Phe Glu Gln 325 330 335 tgt gta gaa agc att gtg gcc agt gag aga gtg gaa gaa ttc gat tat 1056 Cys Val Glu Ser Ile Val Ala Ser Glu Arg Val Glu Glu Phe Asp Tyr 340 345 350 gaa cgt ctg tgt agc gcg ctt gaa caa ctt gcg gag ttt gag aga aag 1104 Glu Arg Leu Cys Ser Ala Leu Glu Gln Leu Ala Glu Phe Glu Arg Lys 355 360 365 gca aat gaa agg gtg gtt cag tca gga gtg ttg aag ggt tta aat gcg 1152 Ala Asn Glu Arg Val Val Gln Ser Gly Val Leu Lys Gly Leu Asn Ala 370 375 380 gag gat ata aaa agg gct gga cag agc ctc att ctg caa gat ggt tgc 1200 Glu Asp Ile Lys Arg Ala Gly Gln Ser Leu Ile Leu Gln Asp Gly Cys 385 390 395 400 aga agc ttc ttt cag aag att gtg aaa aat aaa aat ctg aaa act gat 1248 Arg Ser Phe Phe Gln Lys Ile Val Lys Asn Lys Asn Leu Lys Thr Asp 405 410 415 gtt cat gtg ctt tca tac tgc tgg tgt aat gac ctc att gta tca gct 1296 Val His Val Leu Ser Tyr Cys Trp Cys Asn Asp Leu Ile Val Ser Ala 420 425 430 ttc tct tca gga gat ttg aat gtc ttg aat gta cat tca aat gag ttg 1344 Phe Ser Ser Gly Asp Leu Asn Val Leu Asn Val His Ser Asn Glu Leu 435 440 445 gtt tat caa gaa tct gtc aca act ggt gaa att gta aag aag atg gag 1392 Val Tyr Gln Glu Ser Val Thr Thr Gly Glu Ile Val Lys Lys Met Glu 450 455 460 tct ccc atg gaa aag ctt caa gtc ttc aac gac gtc cta atc gac cgc 1440 Ser Pro Met Glu Lys Leu Gln Val Phe Asn Asp Val Leu Ile Asp Arg 465 470 475 480 agg ggc gaa ggc aat aaa cac ttg aca gtt tac att gga ggc tca gtg 1488 Arg Gly Glu Gly Asn Lys His Leu Thr Val Tyr Ile Gly Gly Ser Val 485 490 495 ggt gac ttg ctt tgc ctg ctt gaa gca gat ata ggc att gta gtt ggt 1536 Gly Asp Leu Leu Cys Leu Leu Glu Ala Asp Ile Gly Ile Val Val Gly 500 505 510 tca agt tca agc cta agg aga cta ggt gat cat ttt ggt gtt tcc ttt 1584 Ser Ser Ser Ser Leu Arg Arg Leu Gly Asp His Phe Gly Val Ser Phe 515 520 525 gtc cca ttg ttc tct ggc ttg gtg aag agg cag aaa gaa ctt gct gat 1632 Val Pro Leu Phe Ser Gly Leu Val Lys Arg Gln Lys Glu Leu Ala Asp 530 535 540 caa gat tgt gcc tct aat tgg tgg aaa cca ttg tct ggt gtt ctt tat 1680 Gln Asp Cys Ala Ser Asn Trp Trp Lys Pro Leu Ser Gly Val Leu Tyr 545 550 555 560 acg gtg tct agt tgg gct gaa ata cag gca ttc att ttg ggt aca tag 1728 Thr Val Ser Ser Trp Ala Glu Ile Gln Ala Phe Ile Leu Gly Thr 565 570 575 <210> 143 <211> 575 <212> PRT <213> Prunus persica <400> 143 Met Ala Ala Leu Ala Arg His Ser Ile Val Arg Leu Asn His Glu Gly 1 5 10 15 Gly Leu Ala Arg Arg Leu Trp Phe Lys Phe Arg Asp Asp Ser Val Phe 20 25 30 Ser Leu Tyr Thr Pro Phe Phe Val Gly Leu Ala Ser Ala Thr Leu His 35 40 45 Ser Glu Thr Thr Phe Arg His Phe Ile Ser Gln Asp Leu His Phe Leu 50 55 60 Lys Ala Phe Val Leu Ala Tyr Glu Leu Ala Glu Asp Cys Ala Asp Asp 65 70 75 80 Glu Asp Asp Lys Asn Gly Leu Arg Asp Leu Arg Lys Arg Ala Val Gly 85 90 95 Arg Leu Gln Met His Asp Thr Phe Val Arg Glu Trp Gly Phe Glu Phe 100 105 110 Pro Asn Glu Asp Ile Ser Lys Asp Ile Ala Thr Thr Lys Tyr Thr Asp 115 120 125 Phe Leu Leu Ala Thr Ala Ser Gly Lys Ile Glu Gly Glu Arg Ser Val 130 135 140 Leu Asp Lys Ile Ala Thr Pro Phe Glu Lys Thr Lys Val Ala Ala Tyr 145 150 155 160 Thr Leu Ala Ala Leu Ala Pro Cys Met Arg Leu Tyr Ala Phe Ile Ser 165 170 175 Thr Glu Ile Gln Gly Ile Ile Asn Pro Asp Gln Asp Ser Thr His Ile 180 185 190 Tyr Lys Ser Trp Ile Glu Asn Tyr Ser Ser Gln Val Phe Glu Glu Ile 195 200 205 Ala Leu Gln Asn Glu Asp Met Leu Asp Lys Leu Ser Val Ser Leu Thr 210 215 220 Gly Glu Glu Leu Glu Ile Ile Glu Lys Leu Tyr His Gln Ala Met Lys 225 230 235 240 Leu Gln Val Asp Phe Ile Ala Ala Gln Pro Ile Ser Asp Gln Gln Ser 245 250 255 Val Val Pro Leu Ser Arg Val His Asp Phe Ser Lys Arg His Leu Thr 260 265 270 Ile Leu Cys Asp Phe Asp Leu Ala Cys Thr Ala Phe Asp Ser Ala Ala 275 280 285 Ile Leu Ala Glu Ile Ala Ile Ile Thr Ala Pro Lys Ala Asp Met Asp 290 295 300 Gly Ser Asp Gln Thr Gln Leu Ala Arg Met Pro Ser Ala Asp Leu Arg 305 310 315 320 Ser Thr Trp Asp Val Leu Ser Thr Gln Tyr Thr Glu Gln Phe Glu Gln 325 330 335 Cys Val Glu Ser Ile Val Ala Ser Glu Arg Val Glu Glu Phe Asp Tyr 340 345 350 Glu Arg Leu Cys Ser Ala Leu Glu Gln Leu Ala Glu Phe Glu Arg Lys 355 360 365 Ala Asn Glu Arg Val Val Gln Ser Gly Val Leu Lys Gly Leu Asn Ala 370 375 380 Glu Asp Ile Lys Arg Ala Gly Gln Ser Leu Ile Leu Gln Asp Gly Cys 385 390 395 400 Arg Ser Phe Phe Gln Lys Ile Val Lys Asn Lys Asn Leu Lys Thr Asp 405 410 415 Val His Val Leu Ser Tyr Cys Trp Cys Asn Asp Leu Ile Val Ser Ala 420 425 430 Phe Ser Ser Gly Asp Leu Asn Val Leu Asn Val His Ser Asn Glu Leu 435 440 445 Val Tyr Gln Glu Ser Val Thr Thr Gly Glu Ile Val Lys Lys Met Glu 450 455 460 Ser Pro Met Glu Lys Leu Gln Val Phe Asn Asp Val Leu Ile Asp Arg 465 470 475 480 Arg Gly Glu Gly Asn Lys His Leu Thr Val Tyr Ile Gly Gly Ser Val 485 490 495 Gly Asp Leu Leu Cys Leu Leu Glu Ala Asp Ile Gly Ile Val Val Gly 500 505 510 Ser Ser Ser Ser Leu Arg Arg Leu Gly Asp His Phe Gly Val Ser Phe 515 520 525 Val Pro Leu Phe Ser Gly Leu Val Lys Arg Gln Lys Glu Leu Ala Asp 530 535 540 Gln Asp Cys Ala Ser Asn Trp Trp Lys Pro Leu Ser Gly Val Leu Tyr 545 550 555 560 Thr Val Ser Ser Trp Ala Glu Ile Gln Ala Phe Ile Leu Gly Thr 565 570 575 <210> 144 <211> 1944 <212> DNA <213> Phoenix dactylifera <220> <221> CDS <222> (1)..(1944) <223> Phoenix_dactylifera gene encoding TMP phosphatase [XP_008796407] <400> 144 atg cga ttc ctc tcc cct ctt ctc ccc ctc cgc cga aac cca aac cct 48 Met Arg Phe Leu Ser Pro Leu Leu Pro Leu Arg Arg Asn Pro Asn Pro 1 5 10 15 agc cct agg ttc ttc tcg ctc tcc cct ccc ata tcc ctc gcc tcc gcc 96 Ser Pro Arg Phe Phe Ser Leu Ser Pro Pro Ile Ser Leu Ala Ser Ala 20 25 30 tgc ccc cga ttc ggt ttc ttg aat cga gat cgc ccc cgg cgc cgc ctt 144 Cys Pro Arg Phe Gly Phe Leu Asn Arg Asp Arg Pro Arg Arg Arg Leu 35 40 45 cca aag ggg ttc cga tcg atc gcc gcg gcg aat cag cgg gcg tcg cct 192 Pro Lys Gly Phe Arg Ser Ile Ala Ala Ala Asn Gln Arg Ala Ser Pro 50 55 60 cca aga ttg gtg ccg gag agg gcg gcc gcc acg agt tct tgg cct tct 240 Pro Arg Leu Val Pro Glu Arg Ala Ala Ala Thr Ser Ser Trp Pro Ser 65 70 75 80 tca gcc gga cga gcc atg gca gtg gtg gcg acg gcg gtt gaa gaa ggc 288 Ser Ala Gly Arg Ala Met Ala Val Val Ala Thr Ala Val Glu Glu Gly 85 90 95 tcc gcg gcg aag cgg ttc tgg atc agg tcc cgg aag gag gcg gtg ttc 336 Ser Ala Ala Lys Arg Phe Trp Ile Arg Ser Arg Lys Glu Ala Val Phe 100 105 110 gcg gag tac acc ccg ttc gtg gtg tgc ctg gcg gcg ggg aga ctg gag 384 Ala Glu Tyr Thr Pro Phe Val Val Cys Leu Ala Ala Gly Arg Leu Glu 115 120 125 atg gag gcc ttc cgc gac tac att gct cag gac gtg cac ttc ctc aat 432 Met Glu Ala Phe Arg Asp Tyr Ile Ala Gln Asp Val His Phe Leu Asn 130 135 140 act ttt gcc caa gcg tat gag atg gcg gaa gag tgt gct gat gat gat 480 Thr Phe Ala Gln Ala Tyr Glu Met Ala Glu Glu Cys Ala Asp Asp Asp 145 150 155 160 gat gcg aag gct gca ata act gat ctg agg aaa gct gtt ttg gag gaa 528 Asp Ala Lys Ala Ala Ile Thr Asp Leu Arg Lys Ala Val Leu Glu Glu 165 170 175 ctg aaa atg cat agt tca ttt gtc caa gaa tgg gga ata gac ccc act 576 Leu Lys Met His Ser Ser Phe Val Gln Glu Trp Gly Ile Asp Pro Thr 180 185 190 aaa gaa atc att cct ttc cct gca aca gta aag tac acc gac ttc ctg 624 Lys Glu Ile Ile Pro Phe Pro Ala Thr Val Lys Tyr Thr Asp Phe Leu 195 200 205 ctt gct aca gct gca gga aaa gtt gaa gga ggg aaa gat cct ggg aaa 672 Leu Ala Thr Ala Ala Gly Lys Val Glu Gly Gly Lys Asp Pro Gly Lys 210 215 220 att gtc act cct ttt gag aag aca aaa att gct gct tat act gta ggt 720 Ile Val Thr Pro Phe Glu Lys Thr Lys Ile Ala Ala Tyr Thr Val Gly 225 230 235 240 gcc atg gct cct tgc atg agg ctt tat gca ttc ttg gga aaa gag ctc 768 Ala Met Ala Pro Cys Met Arg Leu Tyr Ala Phe Leu Gly Lys Glu Leu 245 250 255 cag acg tgt ctg caa ctt gac gaa aat tgt cat ccc tac aaa aag tgg 816 Gln Thr Cys Leu Gln Leu Asp Glu Asn Cys His Pro Tyr Lys Lys Trp 260 265 270 att gat aat tat tcc tct gaa agt ttt gag aca gct gct gtg caa ata 864 Ile Asp Asn Tyr Ser Ser Glu Ser Phe Glu Thr Ala Ala Val Gln Ile 275 280 285 gaa gaa ttg ctt gac aaa ttg agt gtt tca ttg act ggg gag gag ctt 912 Glu Glu Leu Leu Asp Lys Leu Ser Val Ser Leu Thr Gly Glu Glu Leu 290 295 300 gaa gac ata gaa aag ctt tac cgc caa gct atg aaa ctt gaa att gaa 960 Glu Asp Ile Glu Lys Leu Tyr Arg Gln Ala Met Lys Leu Glu Ile Glu 305 310 315 320 ttt ttt ctt gct cag cca att gtc cga cca gct gta gtt cct ttg aca 1008 Phe Phe Leu Ala Gln Pro Ile Val Arg Pro Ala Val Val Pro Leu Thr 325 330 335 aga ctg cat gat ccg gca aat tgc ctt gtc att ttt tct gat ttt gac 1056 Arg Leu His Asp Pro Ala Asn Cys Leu Val Ile Phe Ser Asp Phe Asp 340 345 350 ttg aca tgc agt gta gtt gat tcc tct gcc att tta gca gag att gca 1104 Leu Thr Cys Ser Val Val Asp Ser Ser Ala Ile Leu Ala Glu Ile Ala 355 360 365 ata tta agt gca cca aag act gat aag act ggg act gat aat tta gat 1152 Ile Leu Ser Ala Pro Lys Thr Asp Lys Thr Gly Thr Asp Asn Leu Asp 370 375 380 gct cga agg tct tct tca gaa atg aga aac tca tgg gat gct ctt tct 1200 Ala Arg Arg Ser Ser Ser Glu Met Arg Asn Ser Trp Asp Ala Leu Ser 385 390 395 400 aaa cag tat aca gaa gag tat gag cag tgc ata gaa agc tta ctt cca 1248 Lys Gln Tyr Thr Glu Glu Tyr Glu Gln Cys Ile Glu Ser Leu Leu Pro 405 410 415 tta gaa gaa gct aaa aca ttt gat tat gaa ggc ctt tgc aaa agt ttg 1296 Leu Glu Glu Ala Lys Thr Phe Asp Tyr Glu Gly Leu Cys Lys Ser Leu 420 425 430 ggc cag ctc tct gag ttt gag aaa cga gca aat tcc agg gtt att gag 1344 Gly Gln Leu Ser Glu Phe Glu Lys Arg Ala Asn Ser Arg Val Ile Glu 435 440 445 tct ggg gtg cta aag gga atg aat cta gat gac ata aaa aga gct ggg 1392 Ser Gly Val Leu Lys Gly Met Asn Leu Asp Asp Ile Lys Arg Ala Gly 450 455 460 gaa cgt ttg atc ctc caa gat ggt tgt ata gat ttt ttt cag aag gtt 1440 Glu Arg Leu Ile Leu Gln Asp Gly Cys Ile Asp Phe Phe Gln Lys Val 465 470 475 480 gta aag gaa aag gaa aat cta aat tta gat ctc cat gta ctt tct tat 1488 Val Lys Glu Lys Glu Asn Leu Asn Leu Asp Leu His Val Leu Ser Tyr 485 490 495 tgt tgg tgt gcg gat cta ata agg tca gct ttt tca tca gta ggt tgc 1536 Cys Trp Cys Ala Asp Leu Ile Arg Ser Ala Phe Ser Ser Val Gly Cys 500 505 510 cta aat gat ttg aac ata cac tca aat gag ttc aac tat caa gaa tct 1584 Leu Asn Asp Leu Asn Ile His Ser Asn Glu Phe Asn Tyr Gln Glu Ser 515 520 525 att tca acg ggt gaa att gtt agg aag atg gaa tca ccc atg gac aag 1632 Ile Ser Thr Gly Glu Ile Val Arg Lys Met Glu Ser Pro Met Asp Lys 530 535 540 gtt gaa gca ttc aaa agt atc tta agc aac ctt gga agc aat gag aag 1680 Val Glu Ala Phe Lys Ser Ile Leu Ser Asn Leu Gly Ser Asn Glu Lys 545 550 555 560 cgc tta tct gtg tac att gga gat tcg gtt ggt gac ttg ctt tgc ctg 1728 Arg Leu Ser Val Tyr Ile Gly Asp Ser Val Gly Asp Leu Leu Cys Leu 565 570 575 ttg gaa gca gat gtt ggt att gtg att gga tca agc act agc tta agg 1776 Leu Glu Ala Asp Val Gly Ile Val Ile Gly Ser Ser Thr Ser Leu Arg 580 585 590 aga atc ggg aag cag ttt ggt gtt tct ttc att cca ctc ttc cgt ggt 1824 Arg Ile Gly Lys Gln Phe Gly Val Ser Phe Ile Pro Leu Phe Arg Gly 595 600 605 ttg gta aac aag caa aga caa ctt aat gaa aaa gac tca tct atc tgg 1872 Leu Val Asn Lys Gln Arg Gln Leu Asn Glu Lys Asp Ser Ser Ile Trp 610 615 620 aag ggg ttg tct ggt gtt ctt tat aca gca tca agc tgg tca gaa ata 1920 Lys Gly Leu Ser Gly Val Leu Tyr Thr Ala Ser Ser Trp Ser Glu Ile 625 630 635 640 caa gct ttt att ttg ggg gca taa 1944 Gln Ala Phe Ile Leu Gly Ala 645 <210> 145 <211> 647 <212> PRT <213> Phoenix dactylifera <400> 145 Met Arg Phe Leu Ser Pro Leu Leu Pro Leu Arg Arg Asn Pro Asn Pro 1 5 10 15 Ser Pro Arg Phe Phe Ser Leu Ser Pro Pro Ile Ser Leu Ala Ser Ala 20 25 30 Cys Pro Arg Phe Gly Phe Leu Asn Arg Asp Arg Pro Arg Arg Arg Leu 35 40 45 Pro Lys Gly Phe Arg Ser Ile Ala Ala Ala Asn Gln Arg Ala Ser Pro 50 55 60 Pro Arg Leu Val Pro Glu Arg Ala Ala Ala Thr Ser Ser Trp Pro Ser 65 70 75 80 Ser Ala Gly Arg Ala Met Ala Val Val Ala Thr Ala Val Glu Glu Gly 85 90 95 Ser Ala Ala Lys Arg Phe Trp Ile Arg Ser Arg Lys Glu Ala Val Phe 100 105 110 Ala Glu Tyr Thr Pro Phe Val Val Cys Leu Ala Ala Gly Arg Leu Glu 115 120 125 Met Glu Ala Phe Arg Asp Tyr Ile Ala Gln Asp Val His Phe Leu Asn 130 135 140 Thr Phe Ala Gln Ala Tyr Glu Met Ala Glu Glu Cys Ala Asp Asp Asp 145 150 155 160 Asp Ala Lys Ala Ala Ile Thr Asp Leu Arg Lys Ala Val Leu Glu Glu 165 170 175 Leu Lys Met His Ser Ser Phe Val Gln Glu Trp Gly Ile Asp Pro Thr 180 185 190 Lys Glu Ile Ile Pro Phe Pro Ala Thr Val Lys Tyr Thr Asp Phe Leu 195 200 205 Leu Ala Thr Ala Ala Gly Lys Val Glu Gly Gly Lys Asp Pro Gly Lys 210 215 220 Ile Val Thr Pro Phe Glu Lys Thr Lys Ile Ala Ala Tyr Thr Val Gly 225 230 235 240 Ala Met Ala Pro Cys Met Arg Leu Tyr Ala Phe Leu Gly Lys Glu Leu 245 250 255 Gln Thr Cys Leu Gln Leu Asp Glu Asn Cys His Pro Tyr Lys Lys Trp 260 265 270 Ile Asp Asn Tyr Ser Ser Glu Ser Phe Glu Thr Ala Ala Val Gln Ile 275 280 285 Glu Glu Leu Leu Asp Lys Leu Ser Val Ser Leu Thr Gly Glu Glu Leu 290 295 300 Glu Asp Ile Glu Lys Leu Tyr Arg Gln Ala Met Lys Leu Glu Ile Glu 305 310 315 320 Phe Phe Leu Ala Gln Pro Ile Val Arg Pro Ala Val Val Pro Leu Thr 325 330 335 Arg Leu His Asp Pro Ala Asn Cys Leu Val Ile Phe Ser Asp Phe Asp 340 345 350 Leu Thr Cys Ser Val Val Asp Ser Ser Ala Ile Leu Ala Glu Ile Ala 355 360 365 Ile Leu Ser Ala Pro Lys Thr Asp Lys Thr Gly Thr Asp Asn Leu Asp 370 375 380 Ala Arg Arg Ser Ser Ser Glu Met Arg Asn Ser Trp Asp Ala Leu Ser 385 390 395 400 Lys Gln Tyr Thr Glu Glu Tyr Glu Gln Cys Ile Glu Ser Leu Leu Pro 405 410 415 Leu Glu Glu Ala Lys Thr Phe Asp Tyr Glu Gly Leu Cys Lys Ser Leu 420 425 430 Gly Gln Leu Ser Glu Phe Glu Lys Arg Ala Asn Ser Arg Val Ile Glu 435 440 445 Ser Gly Val Leu Lys Gly Met Asn Leu Asp Asp Ile Lys Arg Ala Gly 450 455 460 Glu Arg Leu Ile Leu Gln Asp Gly Cys Ile Asp Phe Phe Gln Lys Val 465 470 475 480 Val Lys Glu Lys Glu Asn Leu Asn Leu Asp Leu His Val Leu Ser Tyr 485 490 495 Cys Trp Cys Ala Asp Leu Ile Arg Ser Ala Phe Ser Ser Val Gly Cys 500 505 510 Leu Asn Asp Leu Asn Ile His Ser Asn Glu Phe Asn Tyr Gln Glu Ser 515 520 525 Ile Ser Thr Gly Glu Ile Val Arg Lys Met Glu Ser Pro Met Asp Lys 530 535 540 Val Glu Ala Phe Lys Ser Ile Leu Ser Asn Leu Gly Ser Asn Glu Lys 545 550 555 560 Arg Leu Ser Val Tyr Ile Gly Asp Ser Val Gly Asp Leu Leu Cys Leu 565 570 575 Leu Glu Ala Asp Val Gly Ile Val Ile Gly Ser Ser Thr Ser Leu Arg 580 585 590 Arg Ile Gly Lys Gln Phe Gly Val Ser Phe Ile Pro Leu Phe Arg Gly 595 600 605 Leu Val Asn Lys Gln Arg Gln Leu Asn Glu Lys Asp Ser Ser Ile Trp 610 615 620 Lys Gly Leu Ser Gly Val Leu Tyr Thr Ala Ser Ser Trp Ser Glu Ile 625 630 635 640 Gln Ala Phe Ile Leu Gly Ala 645 <210> 146 <211> 1860 <212> DNA <213> Zea mays <220> <221> CDS <222> (1)..(1860) <223> Zea mays gene encoding TMP phosphatase [XP_008678418.1] <400> 146 atg ctt gtt ctc cgc cgt ctc cgc ctc cgc ctc cca ctg cca cgc cct 48 Met Leu Val Leu Arg Arg Leu Arg Leu Arg Leu Pro Leu Pro Arg Pro 1 5 10 15 ctt ctc gtc tcc tcc ttc tcc tcc acc tcc ccc tcc tcc tca ccc tcg 96 Leu Leu Val Ser Ser Phe Ser Ser Thr Ser Pro Ser Ser Ser Pro Ser 20 25 30 acc tct agc tcc tcc tcc tgt tgg tcg tcg aca ggc gaa agt aga agg 144 Thr Ser Ser Ser Ser Ser Cys Trp Ser Ser Thr Gly Glu Ser Arg Arg 35 40 45 gcc atg gcg tca tct cct tct ccc gat tcg gcc gcg gtc gtt gcc gag 192 Ala Met Ala Ser Ser Pro Ser Pro Asp Ser Ala Ala Val Val Ala Glu 50 55 60 ggc tcc gcg gct cgc cgc ttc tgg atc gct gcc tcc acg cgc gag gcc 240 Gly Ser Ala Ala Arg Arg Phe Trp Ile Ala Ala Ser Thr Arg Glu Ala 65 70 75 80 gcc ttc gcc gca tac acg ccc ttc ctc ctc tcc ctc gcc gcc ggc aat 288 Ala Phe Ala Ala Tyr Thr Pro Phe Leu Leu Ser Leu Ala Ala Gly Asn 85 90 95 ctg cgg ctc aac gtg ttt cgc cac tac atc gcg cag gac gcg cac ttc 336 Leu Arg Leu Asn Val Phe Arg His Tyr Ile Ala Gln Asp Ala His Phe 100 105 110 ctt cac gcc ttc gct cgc gcg tac gaa atg gcc gag gac tgc gct gat 384 Leu His Ala Phe Ala Arg Ala Tyr Glu Met Ala Glu Asp Cys Ala Asp 115 120 125 gat gac gac gac atg gcc acc ata gcc gcc ctc agg aag gcc atc ctc 432 Asp Asp Asp Asp Met Ala Thr Ile Ala Ala Leu Arg Lys Ala Ile Leu 130 135 140 caa gag ctc aac ctc cac tcc tcc gtt ctg aag gag tgg gga gtt gat 480 Gln Glu Leu Asn Leu His Ser Ser Val Leu Lys Glu Trp Gly Val Asp 145 150 155 160 cct acc aaa gag ata cct cca agt gca gct aca acc aaa tat act gat 528 Pro Thr Lys Glu Ile Pro Pro Ser Ala Ala Thr Thr Lys Tyr Thr Asp 165 170 175 ttc cta ctt gca act gcg gct gga aaa gtt gat ggc aca aaa ggt tct 576 Phe Leu Leu Ala Thr Ala Ala Gly Lys Val Asp Gly Thr Lys Gly Ser 180 185 190 gac aaa atg gtt act cca ttt gag aag act aaa att gct gca tac act 624 Asp Lys Met Val Thr Pro Phe Glu Lys Thr Lys Ile Ala Ala Tyr Thr 195 200 205 gtt ggg gcc atg act cca tgc atg agg ctt tat gca tat cta ggc aaa 672 Val Gly Ala Met Thr Pro Cys Met Arg Leu Tyr Ala Tyr Leu Gly Lys 210 215 220 gaa ctc atg gtt ttc ctt aaa caa gat gaa aat cat cca tac aag aaa 720 Glu Leu Met Val Phe Leu Lys Gln Asp Glu Asn His Pro Tyr Lys Lys 225 230 235 240 tgg att aac aca tat gca tcc agt gat ttt gag gac acc aca ctc caa 768 Trp Ile Asn Thr Tyr Ala Ser Ser Asp Phe Glu Asp Thr Thr Leu Gln 245 250 255 ata gaa gaa ttg cta gac aaa cta agt gtc tca tta act ggt gag gaa 816 Ile Glu Glu Leu Leu Asp Lys Leu Ser Val Ser Leu Thr Gly Glu Glu 260 265 270 ctt gag att att ggc aag ctc tac cag caa gct atg aaa ctg gaa gtg 864 Leu Glu Ile Ile Gly Lys Leu Tyr Gln Gln Ala Met Lys Leu Glu Val 275 280 285 gag ttc ttt tct tct cag ctt ata gac caa cct gtt gta gct cca ctt 912 Glu Phe Phe Ser Ser Gln Leu Ile Asp Gln Pro Val Val Ala Pro Leu 290 295 300 tca aga tac tgt gat cca aaa tat aaa ctc ttg atc ttt tct gat ttt 960 Ser Arg Tyr Cys Asp Pro Lys Tyr Lys Leu Leu Ile Phe Ser Asp Phe 305 310 315 320 gat ttg acg tgc act att gtt gat tca tct gcc att ttg gcg gag att 1008 Asp Leu Thr Cys Thr Ile Val Asp Ser Ser Ala Ile Leu Ala Glu Ile 325 330 335 gca att ttg tca ttc caa aag gca aat caa agt ggg att gat aat aac 1056 Ala Ile Leu Ser Phe Gln Lys Ala Asn Gln Ser Gly Ile Asp Asn Asn 340 345 350 ctc gac cgt gca aaa tcg gga gac ctg aga agt tcg tgg aac atg ctc 1104 Leu Asp Arg Ala Lys Ser Gly Asp Leu Arg Ser Ser Trp Asn Met Leu 355 360 365 tct aag caa tac atg gaa gag tat gag aaa tgc atg gaa aga cta ctt 1152 Ser Lys Gln Tyr Met Glu Glu Tyr Glu Lys Cys Met Glu Arg Leu Leu 370 375 380 cct cca gaa gaa tcg aag tca cta gat tat gat aaa ctg tat aaa ggc 1200 Pro Pro Glu Glu Ser Lys Ser Leu Asp Tyr Asp Lys Leu Tyr Lys Gly 385 390 395 400 ctg gag gtg cta gct gag ttt gag aag ctt gca aat tct agg gtt gtc 1248 Leu Glu Val Leu Ala Glu Phe Glu Lys Leu Ala Asn Ser Arg Val Val 405 410 415 gac tct ggt gtg ctg agg gga atg aat ttg gaa gac atc agg aaa gct 1296 Asp Ser Gly Val Leu Arg Gly Met Asn Leu Glu Asp Ile Arg Lys Ala 420 425 430 ggt gag cgt ctt att ctt caa ggt ggc tgt aaa aat ttc ttt cag aag 1344 Gly Glu Arg Leu Ile Leu Gln Gly Gly Cys Lys Asn Phe Phe Gln Lys 435 440 445 att gta aaa aca agg gag aac ctc aat ttg gat gtc cat att ctt tcc 1392 Ile Val Lys Thr Arg Glu Asn Leu Asn Leu Asp Val His Ile Leu Ser 450 455 460 tat tgc tgg tgt gca gaa ctt ata aga tca gcc ttc tca tca gcc ggt 1440 Tyr Cys Trp Cys Ala Glu Leu Ile Arg Ser Ala Phe Ser Ser Ala Gly 465 470 475 480 tgt cta gat ggt ttg aac ata cat tca aat gag ttt gcc ttt gag gat 1488 Cys Leu Asp Gly Leu Asn Ile His Ser Asn Glu Phe Ala Phe Glu Asp 485 490 495 tct gtt tca act ggt gag atc gac aga aag atg cag tct ccg cta gac 1536 Ser Val Ser Thr Gly Glu Ile Asp Arg Lys Met Gln Ser Pro Leu Asp 500 505 510 aaa gtt gaa aag ttc aag agc atc aga agt gac gtg gac agt aca gtg 1584 Lys Val Glu Lys Phe Lys Ser Ile Arg Ser Asp Val Asp Ser Thr Val 515 520 525 cca ttc cta tct gtt tat att gga gac tcg gtt gga gat ttg ctc tgc 1632 Pro Phe Leu Ser Val Tyr Ile Gly Asp Ser Val Gly Asp Leu Leu Cys 530 535 540 tta ttg gag gct gat att ggt ata gtc att ggg tca acc aca agt ttg 1680 Leu Leu Glu Ala Asp Ile Gly Ile Val Ile Gly Ser Thr Thr Ser Leu 545 550 555 560 cgt agg gtg ggc aaa cag ttt ggt gtt tct ttt gtc cca ttg ttc cct 1728 Arg Arg Val Gly Lys Gln Phe Gly Val Ser Phe Val Pro Leu Phe Pro 565 570 575 ggt cta gta gag aag cag agg caa ctg gcg gag gaa gat gca tcc gta 1776 Gly Leu Val Glu Lys Gln Arg Gln Leu Ala Glu Glu Asp Ala Ser Val 580 585 590 ttc aag gca cgg tct gga gtc ctc tat acg gtt tct agc tgg tca gaa 1824 Phe Lys Ala Arg Ser Gly Val Leu Tyr Thr Val Ser Ser Trp Ser Glu 595 600 605 ata cac gcc ttc gta ctg gga agt gat ttc agc tga 1860 Ile His Ala Phe Val Leu Gly Ser Asp Phe Ser 610 615 <210> 147 <211> 619 <212> PRT <213> Zea mays <400> 147 Met Leu Val Leu Arg Arg Leu Arg Leu Arg Leu Pro Leu Pro Arg Pro 1 5 10 15 Leu Leu Val Ser Ser Phe Ser Ser Thr Ser Pro Ser Ser Ser Pro Ser 20 25 30 Thr Ser Ser Ser Ser Ser Cys Trp Ser Ser Thr Gly Glu Ser Arg Arg 35 40 45 Ala Met Ala Ser Ser Pro Ser Pro Asp Ser Ala Ala Val Val Ala Glu 50 55 60 Gly Ser Ala Ala Arg Arg Phe Trp Ile Ala Ala Ser Thr Arg Glu Ala 65 70 75 80 Ala Phe Ala Ala Tyr Thr Pro Phe Leu Leu Ser Leu Ala Ala Gly Asn 85 90 95 Leu Arg Leu Asn Val Phe Arg His Tyr Ile Ala Gln Asp Ala His Phe 100 105 110 Leu His Ala Phe Ala Arg Ala Tyr Glu Met Ala Glu Asp Cys Ala Asp 115 120 125 Asp Asp Asp Asp Met Ala Thr Ile Ala Ala Leu Arg Lys Ala Ile Leu 130 135 140 Gln Glu Leu Asn Leu His Ser Ser Val Leu Lys Glu Trp Gly Val Asp 145 150 155 160 Pro Thr Lys Glu Ile Pro Pro Ser Ala Ala Thr Thr Lys Tyr Thr Asp 165 170 175 Phe Leu Leu Ala Thr Ala Ala Gly Lys Val Asp Gly Thr Lys Gly Ser 180 185 190 Asp Lys Met Val Thr Pro Phe Glu Lys Thr Lys Ile Ala Ala Tyr Thr 195 200 205 Val Gly Ala Met Thr Pro Cys Met Arg Leu Tyr Ala Tyr Leu Gly Lys 210 215 220 Glu Leu Met Val Phe Leu Lys Gln Asp Glu Asn His Pro Tyr Lys Lys 225 230 235 240 Trp Ile Asn Thr Tyr Ala Ser Ser Asp Phe Glu Asp Thr Thr Leu Gln 245 250 255 Ile Glu Glu Leu Leu Asp Lys Leu Ser Val Ser Leu Thr Gly Glu Glu 260 265 270 Leu Glu Ile Ile Gly Lys Leu Tyr Gln Gln Ala Met Lys Leu Glu Val 275 280 285 Glu Phe Phe Ser Ser Gln Leu Ile Asp Gln Pro Val Val Ala Pro Leu 290 295 300 Ser Arg Tyr Cys Asp Pro Lys Tyr Lys Leu Leu Ile Phe Ser Asp Phe 305 310 315 320 Asp Leu Thr Cys Thr Ile Val Asp Ser Ser Ala Ile Leu Ala Glu Ile 325 330 335 Ala Ile Leu Ser Phe Gln Lys Ala Asn Gln Ser Gly Ile Asp Asn Asn 340 345 350 Leu Asp Arg Ala Lys Ser Gly Asp Leu Arg Ser Ser Trp Asn Met Leu 355 360 365 Ser Lys Gln Tyr Met Glu Glu Tyr Glu Lys Cys Met Glu Arg Leu Leu 370 375 380 Pro Pro Glu Glu Ser Lys Ser Leu Asp Tyr Asp Lys Leu Tyr Lys Gly 385 390 395 400 Leu Glu Val Leu Ala Glu Phe Glu Lys Leu Ala Asn Ser Arg Val Val 405 410 415 Asp Ser Gly Val Leu Arg Gly Met Asn Leu Glu Asp Ile Arg Lys Ala 420 425 430 Gly Glu Arg Leu Ile Leu Gln Gly Gly Cys Lys Asn Phe Phe Gln Lys 435 440 445 Ile Val Lys Thr Arg Glu Asn Leu Asn Leu Asp Val His Ile Leu Ser 450 455 460 Tyr Cys Trp Cys Ala Glu Leu Ile Arg Ser Ala Phe Ser Ser Ala Gly 465 470 475 480 Cys Leu Asp Gly Leu Asn Ile His Ser Asn Glu Phe Ala Phe Glu Asp 485 490 495 Ser Val Ser Thr Gly Glu Ile Asp Arg Lys Met Gln Ser Pro Leu Asp 500 505 510 Lys Val Glu Lys Phe Lys Ser Ile Arg Ser Asp Val Asp Ser Thr Val 515 520 525 Pro Phe Leu Ser Val Tyr Ile Gly Asp Ser Val Gly Asp Leu Leu Cys 530 535 540 Leu Leu Glu Ala Asp Ile Gly Ile Val Ile Gly Ser Thr Thr Ser Leu 545 550 555 560 Arg Arg Val Gly Lys Gln Phe Gly Val Ser Phe Val Pro Leu Phe Pro 565 570 575 Gly Leu Val Glu Lys Gln Arg Gln Leu Ala Glu Glu Asp Ala Ser Val 580 585 590 Phe Lys Ala Arg Ser Gly Val Leu Tyr Thr Val Ser Ser Trp Ser Glu 595 600 605 Ile His Ala Phe Val Leu Gly Ser Asp Phe Ser 610 615 <210> 148 <211> 1830 <212> DNA <213> Oryza sativa <220> <221> CDS <222> (1)..(1830) <223> Oryza sativa gene encoding TMP phosphatase [NP_001062539.1] <400> 148 atg cgc ggc ctc ctc cgc cgt gtc tac ctc cgc ctc ccc cct ttc cct 48 Met Arg Gly Leu Leu Arg Arg Val Tyr Leu Arg Leu Pro Pro Phe Pro 1 5 10 15 cct gcc acc tct ctt tat tat tgg tca aga aca aga cct gca gct gca 96 Pro Ala Thr Ser Leu Tyr Tyr Trp Ser Arg Thr Arg Pro Ala Ala Ala 20 25 30 ggg ccc aac cac ccc atc cct agg cgc atg tcg acg tcc tct act gcc 144 Gly Pro Asn His Pro Ile Pro Arg Arg Met Ser Thr Ser Ser Thr Ala 35 40 45 gcg gcg gtc gtt gcc gag ggc tcc gcc gct cgc cgc ttc tgg atc gcc 192 Ala Ala Val Val Ala Glu Gly Ser Ala Ala Arg Arg Phe Trp Ile Ala 50 55 60 gcc gcc tcg agg gag gcc gcc ttc gcc gcc tac acg ccc ttc ctc gtc 240 Ala Ala Ser Arg Glu Ala Ala Phe Ala Ala Tyr Thr Pro Phe Leu Val 65 70 75 80 tcc ctc gcc gcc ggg gcc ctc cgc ctg gat tcc ttc cgc caa tac atc 288 Ser Leu Ala Ala Gly Ala Leu Arg Leu Asp Ser Phe Arg Gln Tyr Ile 85 90 95 gcc cag gat gcc tac ttc ctc cac gcc ttc gcc cgc gcc tat gag atg 336 Ala Gln Asp Ala Tyr Phe Leu His Ala Phe Ala Arg Ala Tyr Glu Met 100 105 110 gcc gag gag tgc gcc gat gac gac gac gac aag gcc acc atc gtc gtc 384 Ala Glu Glu Cys Ala Asp Asp Asp Asp Asp Lys Ala Thr Ile Val Val 115 120 125 ctc agg aag gcc atc ctc cgc gag ctc aac ctc cac gct tcc gtc ctt 432 Leu Arg Lys Ala Ile Leu Arg Glu Leu Asn Leu His Ala Ser Val Leu 130 135 140 cag gaa tgg gga gtc gat ccc aac aaa gaa atc cct cca atc cca gcc 480 Gln Glu Trp Gly Val Asp Pro Asn Lys Glu Ile Pro Pro Ile Pro Ala 145 150 155 160 aca act aag tac act gat ttc tta ctt gca act tcc act gga aag gtt 528 Thr Thr Lys Tyr Thr Asp Phe Leu Leu Ala Thr Ser Thr Gly Lys Val 165 170 175 gat ggt ggg aaa ggt tct gat aaa atg gtc aca cca ttc gag aag acg 576 Asp Gly Gly Lys Gly Ser Asp Lys Met Val Thr Pro Phe Glu Lys Thr 180 185 190 aaa att gct gca tac act gtt ggg gct atg acc cca tgc atg agg ctt 624 Lys Ile Ala Ala Tyr Thr Val Gly Ala Met Thr Pro Cys Met Arg Leu 195 200 205 tat gcg tat ctg ggc aaa gaa ctt gca gtt ttc ttg aaa cag gat gaa 672 Tyr Ala Tyr Leu Gly Lys Glu Leu Ala Val Phe Leu Lys Gln Asp Glu 210 215 220 aat cac cca tac aag aaa tgg att gag act tat gca tcc agt gat ttt 720 Asn His Pro Tyr Lys Lys Trp Ile Glu Thr Tyr Ala Ser Ser Asp Phe 225 230 235 240 gag aat aac gca ctc caa ata gaa gag ttg ctt gat aaa cta agt gtc 768 Glu Asn Asn Ala Leu Gln Ile Glu Glu Leu Leu Asp Lys Leu Ser Val 245 250 255 tct cta act ggc gag gag ctt gag att att ggg aag ctc tac cag caa 816 Ser Leu Thr Gly Glu Glu Leu Glu Ile Ile Gly Lys Leu Tyr Gln Gln 260 265 270 gct atg agg ctg gaa gtt gag ttc ttc tct gct cag cca gta gac caa 864 Ala Met Arg Leu Glu Val Glu Phe Phe Ser Ala Gln Pro Val Asp Gln 275 280 285 cct gtt gta gct cca ctc tca aga tat tgt ggt ccg aaa gat aag ctc 912 Pro Val Val Ala Pro Leu Ser Arg Tyr Cys Gly Pro Lys Asp Lys Leu 290 295 300 ttg ata ttt tgt gat ttt gat ttg aca tgc act gtt gtt gat tca tct 960 Leu Ile Phe Cys Asp Phe Asp Leu Thr Cys Thr Val Val Asp Ser Ser 305 310 315 320 gcc att ttg gcg gag att gca atc ttg tca cac caa aag gct agt caa 1008 Ala Ile Leu Ala Glu Ile Ala Ile Leu Ser His Gln Lys Ala Ser Gln 325 330 335 ggt ggg gct gat agt tcc ctt gat cgt aca aaa tca gcg gac ttg aga 1056 Gly Gly Ala Asp Ser Ser Leu Asp Arg Thr Lys Ser Ala Asp Leu Arg 340 345 350 aat tca tgg aac atg ctc tca aat caa tac atg gaa gag tat gag caa 1104 Asn Ser Trp Asn Met Leu Ser Asn Gln Tyr Met Glu Glu Tyr Glu Gln 355 360 365 tgc ata gca agc ttg ctt cct cca gaa gaa gca agg tca cta gac tat 1152 Cys Ile Ala Ser Leu Leu Pro Pro Glu Glu Ala Arg Ser Leu Asp Tyr 370 375 380 gat caa ctg tat aaa ggt ttg gag gtg cta tcg cag ttt gag aaa ctt 1200 Asp Gln Leu Tyr Lys Gly Leu Glu Val Leu Ser Gln Phe Glu Lys Leu 385 390 395 400 gca aac tct agg gtg gtt gat tct ggt gtc ctg agg gga atg aat tta 1248 Ala Asn Ser Arg Val Val Asp Ser Gly Val Leu Arg Gly Met Asn Leu 405 410 415 gat gac atc cga aaa gct gga gag agg ctt att ctg caa gat gga tgc 1296 Asp Asp Ile Arg Lys Ala Gly Glu Arg Leu Ile Leu Gln Asp Gly Cys 420 425 430 aaa att ttt ttt caa aag att ggc aaa aca agg gag aac ctc aat tta 1344 Lys Ile Phe Phe Gln Lys Ile Gly Lys Thr Arg Glu Asn Leu Asn Leu 435 440 445 gat gtc cat att ctt tcc tat tgc tgg tgc gca gat ctt ata agg tca 1392 Asp Val His Ile Leu Ser Tyr Cys Trp Cys Ala Asp Leu Ile Arg Ser 450 455 460 gct ttt tca tca gtt ggt tgt cta gac ggg ctg aac ata cat tca aat 1440 Ala Phe Ser Ser Val Gly Cys Leu Asp Gly Leu Asn Ile His Ser Asn 465 470 475 480 gag ttt gct ttt gag gga tct gtt tca act ggt cat att aac aga caa 1488 Glu Phe Ala Phe Glu Gly Ser Val Ser Thr Gly His Ile Asn Arg Gln 485 490 495 atg gag tct cct ctg gac aaa gct gaa aag ttc aag agc atc aaa agc 1536 Met Glu Ser Pro Leu Asp Lys Ala Glu Lys Phe Lys Ser Ile Lys Ser 500 505 510 gac gtg ggt agt aca ggg aca tta ttg tca gtc tat att ggg gac tcg 1584 Asp Val Gly Ser Thr Gly Thr Leu Leu Ser Val Tyr Ile Gly Asp Ser 515 520 525 gtt gga gat ttg ctt tgc ttg ttg gag gca gat att ggt att gtt gtt 1632 Val Gly Asp Leu Leu Cys Leu Leu Glu Ala Asp Ile Gly Ile Val Val 530 535 540 gga tca agc aca acc ttg cgg aga gtg ggc aaa cag ttt ggt gtt tca 1680 Gly Ser Ser Thr Thr Leu Arg Arg Val Gly Lys Gln Phe Gly Val Ser 545 550 555 560 ttt gtt cct ctg ttc act ggg ttg gta gag aag cag agg cga ata gaa 1728 Phe Val Pro Leu Phe Thr Gly Leu Val Glu Lys Gln Arg Arg Ile Glu 565 570 575 aag gaa gaa tca tcc atc ttc aag gca cgg tct gga att ctt tat acg 1776 Lys Glu Glu Ser Ser Ile Phe Lys Ala Arg Ser Gly Ile Leu Tyr Thr 580 585 590 gtt tct agc tgg tcg gag gta cag gct ttc atc ctg gga aat gat ttc 1824 Val Ser Ser Trp Ser Glu Val Gln Ala Phe Ile Leu Gly Asn Asp Phe 595 600 605 agc tga 1830 Ser <210> 149 <211> 609 <212> PRT <213> Oryza sativa <400> 149 Met Arg Gly Leu Leu Arg Arg Val Tyr Leu Arg Leu Pro Pro Phe Pro 1 5 10 15 Pro Ala Thr Ser Leu Tyr Tyr Trp Ser Arg Thr Arg Pro Ala Ala Ala 20 25 30 Gly Pro Asn His Pro Ile Pro Arg Arg Met Ser Thr Ser Ser Thr Ala 35 40 45 Ala Ala Val Val Ala Glu Gly Ser Ala Ala Arg Arg Phe Trp Ile Ala 50 55 60 Ala Ala Ser Arg Glu Ala Ala Phe Ala Ala Tyr Thr Pro Phe Leu Val 65 70 75 80 Ser Leu Ala Ala Gly Ala Leu Arg Leu Asp Ser Phe Arg Gln Tyr Ile 85 90 95 Ala Gln Asp Ala Tyr Phe Leu His Ala Phe Ala Arg Ala Tyr Glu Met 100 105 110 Ala Glu Glu Cys Ala Asp Asp Asp Asp Asp Lys Ala Thr Ile Val Val 115 120 125 Leu Arg Lys Ala Ile Leu Arg Glu Leu Asn Leu His Ala Ser Val Leu 130 135 140 Gln Glu Trp Gly Val Asp Pro Asn Lys Glu Ile Pro Pro Ile Pro Ala 145 150 155 160 Thr Thr Lys Tyr Thr Asp Phe Leu Leu Ala Thr Ser Thr Gly Lys Val 165 170 175 Asp Gly Gly Lys Gly Ser Asp Lys Met Val Thr Pro Phe Glu Lys Thr 180 185 190 Lys Ile Ala Ala Tyr Thr Val Gly Ala Met Thr Pro Cys Met Arg Leu 195 200 205 Tyr Ala Tyr Leu Gly Lys Glu Leu Ala Val Phe Leu Lys Gln Asp Glu 210 215 220 Asn His Pro Tyr Lys Lys Trp Ile Glu Thr Tyr Ala Ser Ser Asp Phe 225 230 235 240 Glu Asn Asn Ala Leu Gln Ile Glu Glu Leu Leu Asp Lys Leu Ser Val 245 250 255 Ser Leu Thr Gly Glu Glu Leu Glu Ile Ile Gly Lys Leu Tyr Gln Gln 260 265 270 Ala Met Arg Leu Glu Val Glu Phe Phe Ser Ala Gln Pro Val Asp Gln 275 280 285 Pro Val Val Ala Pro Leu Ser Arg Tyr Cys Gly Pro Lys Asp Lys Leu 290 295 300 Leu Ile Phe Cys Asp Phe Asp Leu Thr Cys Thr Val Val Asp Ser Ser 305 310 315 320 Ala Ile Leu Ala Glu Ile Ala Ile Leu Ser His Gln Lys Ala Ser Gln 325 330 335 Gly Gly Ala Asp Ser Ser Leu Asp Arg Thr Lys Ser Ala Asp Leu Arg 340 345 350 Asn Ser Trp Asn Met Leu Ser Asn Gln Tyr Met Glu Glu Tyr Glu Gln 355 360 365 Cys Ile Ala Ser Leu Leu Pro Pro Glu Glu Ala Arg Ser Leu Asp Tyr 370 375 380 Asp Gln Leu Tyr Lys Gly Leu Glu Val Leu Ser Gln Phe Glu Lys Leu 385 390 395 400 Ala Asn Ser Arg Val Val Asp Ser Gly Val Leu Arg Gly Met Asn Leu 405 410 415 Asp Asp Ile Arg Lys Ala Gly Glu Arg Leu Ile Leu Gln Asp Gly Cys 420 425 430 Lys Ile Phe Phe Gln Lys Ile Gly Lys Thr Arg Glu Asn Leu Asn Leu 435 440 445 Asp Val His Ile Leu Ser Tyr Cys Trp Cys Ala Asp Leu Ile Arg Ser 450 455 460 Ala Phe Ser Ser Val Gly Cys Leu Asp Gly Leu Asn Ile His Ser Asn 465 470 475 480 Glu Phe Ala Phe Glu Gly Ser Val Ser Thr Gly His Ile Asn Arg Gln 485 490 495 Met Glu Ser Pro Leu Asp Lys Ala Glu Lys Phe Lys Ser Ile Lys Ser 500 505 510 Asp Val Gly Ser Thr Gly Thr Leu Leu Ser Val Tyr Ile Gly Asp Ser 515 520 525 Val Gly Asp Leu Leu Cys Leu Leu Glu Ala Asp Ile Gly Ile Val Val 530 535 540 Gly Ser Ser Thr Thr Leu Arg Arg Val Gly Lys Gln Phe Gly Val Ser 545 550 555 560 Phe Val Pro Leu Phe Thr Gly Leu Val Glu Lys Gln Arg Arg Ile Glu 565 570 575 Lys Glu Glu Ser Ser Ile Phe Lys Ala Arg Ser Gly Ile Leu Tyr Thr 580 585 590 Val Ser Ser Trp Ser Glu Val Gln Ala Phe Ile Leu Gly Asn Asp Phe 595 600 605 Ser <210> 150 <211> 1683 <212> DNA <213> Picea sitchensis <220> <221> CDS <222> (1)..(1683) <223> Picea_sitchensis gene encoding TMP phosphatase [ABR16455] <400> 150 atg ggg gtc gcc gat gaa gca gga gtc gcc aga agg cta tgg aca aag 48 Met Gly Val Ala Asp Glu Ala Gly Val Ala Arg Arg Leu Trp Thr Lys 1 5 10 15 ttc aag aaa gac acc gcg ctt gca cag tat aat tcc ttt gtt gtt gct 96 Phe Lys Lys Asp Thr Ala Leu Ala Gln Tyr Asn Ser Phe Val Val Ala 20 25 30 ttg gcg gcc ggg acg ctc aac atg acg tct ttt cag cag tac atg gcg 144 Leu Ala Ala Gly Thr Leu Asn Met Thr Ser Phe Gln Gln Tyr Met Ala 35 40 45 cag gat gct tat ttt ctc aaa gca ttt gct cag gcg tac aca atg gca 192 Gln Asp Ala Tyr Phe Leu Lys Ala Phe Ala Gln Ala Tyr Thr Met Ala 50 55 60 gag gat tgc gca gat gat gac gac gac aaa gca tcg atc cgt gaa cta 240 Glu Asp Cys Ala Asp Asp Asp Asp Asp Lys Ala Ser Ile Arg Glu Leu 65 70 75 80 cga aaa gcc gct gag gaa gag ctc aat ctg cac aat tcc ttg gct gag 288 Arg Lys Ala Ala Glu Glu Glu Leu Asn Leu His Asn Ser Leu Ala Glu 85 90 95 gac tgg gac gtt gaa ttt gca aaa gag tgc tct ccc aat atg gca aca 336 Asp Trp Asp Val Glu Phe Ala Lys Glu Cys Ser Pro Asn Met Ala Thr 100 105 110 gtc aag tac aca gaa ttt tta ttg gca aca gct gct ggc aag gtg gaa 384 Val Lys Tyr Thr Glu Phe Leu Leu Ala Thr Ala Ala Gly Lys Val Glu 115 120 125 gga ggg aag gga cca agc aga agt gtg act cct ttt gag aaa aca aaa 432 Gly Gly Lys Gly Pro Ser Arg Ser Val Thr Pro Phe Glu Lys Thr Lys 130 135 140 ata gca gca tac aca gtg ggt gcc atg acc ccg tgc atg agg ctt tat 480 Ile Ala Ala Tyr Thr Val Gly Ala Met Thr Pro Cys Met Arg Leu Tyr 145 150 155 160 gct ttc ttg ggc caa gaa att gtc aaa gcc ctg gaa cct gat tgc agt 528 Ala Phe Leu Gly Gln Glu Ile Val Lys Ala Leu Glu Pro Asp Cys Ser 165 170 175 aat cat cca tat aag cag tgg att gaa aca tac tct tct gca aag ttt 576 Asn His Pro Tyr Lys Gln Trp Ile Glu Thr Tyr Ser Ser Ala Lys Phe 180 185 190 gag gca tcg gca tta caa act gaa gag ttg ctt gac aaa ctg gct att 624 Glu Ala Ser Ala Leu Gln Thr Glu Glu Leu Leu Asp Lys Leu Ala Ile 195 200 205 tcg cta act ggg gaa gag ctt gaa gtg ctg cgg agg ttg tat tat cat 672 Ser Leu Thr Gly Glu Glu Leu Glu Val Leu Arg Arg Leu Tyr Tyr His 210 215 220 gcc tta aaa cta gaa ata gaa ttc ttt tcc gct cag cct ttc tct cag 720 Ala Leu Lys Leu Glu Ile Glu Phe Phe Ser Ala Gln Pro Phe Ser Gln 225 230 235 240 aga aca tta gtt ccg atg ttg aaa ctg ggt gat tca gcc agc cgc cga 768 Arg Thr Leu Val Pro Met Leu Lys Leu Gly Asp Ser Ala Ser Arg Arg 245 250 255 tat acc att gtc tca gat ttc gat ttg tct tgc act gtc ttg gat tct 816 Tyr Thr Ile Val Ser Asp Phe Asp Leu Ser Cys Thr Val Leu Asp Ser 260 265 270 tca gca gta tta gca gaa att gca ata ttg act act ctc aaa act gag 864 Ser Ala Val Leu Ala Glu Ile Ala Ile Leu Thr Thr Leu Lys Thr Glu 275 280 285 caa aat ggt gct gaa aac tta agt gat cac aag tca tca tcg gag ttg 912 Gln Asn Gly Ala Glu Asn Leu Ser Asp His Lys Ser Ser Ser Glu Leu 290 295 300 aga aaa act tgg gat gca ctt tct agt caa tat tct gaa gaa tgt gaa 960 Arg Lys Thr Trp Asp Ala Leu Ser Ser Gln Tyr Ser Glu Glu Cys Glu 305 310 315 320 gaa tgc tta agg aag act ctg cca cct gaa gaa gtg ggc tct ttt gat 1008 Glu Cys Leu Arg Lys Thr Leu Pro Pro Glu Glu Val Gly Ser Phe Asp 325 330 335 tat gaa ggc cta cac caa tct ctt gag cat ctg tct cag ttt gaa atg 1056 Tyr Glu Gly Leu His Gln Ser Leu Glu His Leu Ser Gln Phe Glu Met 340 345 350 gag gca aac tct aaa gtt gtc gag tca ggt gtc ctt gag ggc att aat 1104 Glu Ala Asn Ser Lys Val Val Glu Ser Gly Val Leu Glu Gly Ile Asn 355 360 365 ata gat gac att aaa aag gca gga gag cgt ctt gca ttt cag gat gga 1152 Ile Asp Asp Ile Lys Lys Ala Gly Glu Arg Leu Ala Phe Gln Asp Gly 370 375 380 tgc gca aac ttt ttt gaa caa atc cta acg aaa atg gac agc tta aat 1200 Cys Ala Asn Phe Phe Glu Gln Ile Leu Thr Lys Met Asp Ser Leu Asn 385 390 395 400 gtg gat gtg cac ata att tct gtt tgt tgg agt gga gat atc atc agg 1248 Val Asp Val His Ile Ile Ser Val Cys Trp Ser Gly Asp Ile Ile Arg 405 410 415 gct gct ttt tca tca agc ggt ttg gat ggt tta cag gtt cat tca aat 1296 Ala Ala Phe Ser Ser Ser Gly Leu Asp Gly Leu Gln Val His Ser Asn 420 425 430 gaa ctc acc ttt gtg gaa tca gtc tct act ggt ggt att gat agg cgt 1344 Glu Leu Thr Phe Val Glu Ser Val Ser Thr Gly Gly Ile Asp Arg Arg 435 440 445 gtt gag tcc cca gtt gac aag ttg aaa atc ttc aat aat att tgg agt 1392 Val Glu Ser Pro Val Asp Lys Leu Lys Ile Phe Asn Asn Ile Trp Ser 450 455 460 tct tca aag gac cag gac acg gaa cat atc tct ata tac att ggg gac 1440 Ser Ser Lys Asp Gln Asp Thr Glu His Ile Ser Ile Tyr Ile Gly Asp 465 470 475 480 ggt tta ggt gac ttg ctt tgt ctt ctt cag gca gat att gga ata gtg 1488 Gly Leu Gly Asp Leu Leu Cys Leu Leu Gln Ala Asp Ile Gly Ile Val 485 490 495 att ggt aca agc tca acg cta aga agg gtt gga aaa cgt ttt gga gta 1536 Ile Gly Thr Ser Ser Thr Leu Arg Arg Val Gly Lys Arg Phe Gly Val 500 505 510 tcc ttt gtt cct ttg ttt tct ggt ctt ctc aaa cag gag aga gca tat 1584 Ser Phe Val Pro Leu Phe Ser Gly Leu Leu Lys Gln Glu Arg Ala Tyr 515 520 525 gta gaa ggt tct agt tgt tgg aca aaa caa agt ggt att ctt tat acc 1632 Val Glu Gly Ser Ser Cys Trp Thr Lys Gln Ser Gly Ile Leu Tyr Thr 530 535 540 gtc tct agt tgg agt gaa ata cat gct ttt att ttg ggc tct tcc aat 1680 Val Ser Ser Trp Ser Glu Ile His Ala Phe Ile Leu Gly Ser Ser Asn 545 550 555 560 tga 1683 <210> 151 <211> 560 <212> PRT <213> Picea sitchensis <400> 151 Met Gly Val Ala Asp Glu Ala Gly Val Ala Arg Arg Leu Trp Thr Lys 1 5 10 15 Phe Lys Lys Asp Thr Ala Leu Ala Gln Tyr Asn Ser Phe Val Val Ala 20 25 30 Leu Ala Ala Gly Thr Leu Asn Met Thr Ser Phe Gln Gln Tyr Met Ala 35 40 45 Gln Asp Ala Tyr Phe Leu Lys Ala Phe Ala Gln Ala Tyr Thr Met Ala 50 55 60 Glu Asp Cys Ala Asp Asp Asp Asp Asp Lys Ala Ser Ile Arg Glu Leu 65 70 75 80 Arg Lys Ala Ala Glu Glu Glu Leu Asn Leu His Asn Ser Leu Ala Glu 85 90 95 Asp Trp Asp Val Glu Phe Ala Lys Glu Cys Ser Pro Asn Met Ala Thr 100 105 110 Val Lys Tyr Thr Glu Phe Leu Leu Ala Thr Ala Ala Gly Lys Val Glu 115 120 125 Gly Gly Lys Gly Pro Ser Arg Ser Val Thr Pro Phe Glu Lys Thr Lys 130 135 140 Ile Ala Ala Tyr Thr Val Gly Ala Met Thr Pro Cys Met Arg Leu Tyr 145 150 155 160 Ala Phe Leu Gly Gln Glu Ile Val Lys Ala Leu Glu Pro Asp Cys Ser 165 170 175 Asn His Pro Tyr Lys Gln Trp Ile Glu Thr Tyr Ser Ser Ala Lys Phe 180 185 190 Glu Ala Ser Ala Leu Gln Thr Glu Glu Leu Leu Asp Lys Leu Ala Ile 195 200 205 Ser Leu Thr Gly Glu Glu Leu Glu Val Leu Arg Arg Leu Tyr Tyr His 210 215 220 Ala Leu Lys Leu Glu Ile Glu Phe Phe Ser Ala Gln Pro Phe Ser Gln 225 230 235 240 Arg Thr Leu Val Pro Met Leu Lys Leu Gly Asp Ser Ala Ser Arg Arg 245 250 255 Tyr Thr Ile Val Ser Asp Phe Asp Leu Ser Cys Thr Val Leu Asp Ser 260 265 270 Ser Ala Val Leu Ala Glu Ile Ala Ile Leu Thr Thr Leu Lys Thr Glu 275 280 285 Gln Asn Gly Ala Glu Asn Leu Ser Asp His Lys Ser Ser Ser Glu Leu 290 295 300 Arg Lys Thr Trp Asp Ala Leu Ser Ser Gln Tyr Ser Glu Glu Cys Glu 305 310 315 320 Glu Cys Leu Arg Lys Thr Leu Pro Pro Glu Glu Val Gly Ser Phe Asp 325 330 335 Tyr Glu Gly Leu His Gln Ser Leu Glu His Leu Ser Gln Phe Glu Met 340 345 350 Glu Ala Asn Ser Lys Val Val Glu Ser Gly Val Leu Glu Gly Ile Asn 355 360 365 Ile Asp Asp Ile Lys Lys Ala Gly Glu Arg Leu Ala Phe Gln Asp Gly 370 375 380 Cys Ala Asn Phe Phe Glu Gln Ile Leu Thr Lys Met Asp Ser Leu Asn 385 390 395 400 Val Asp Val His Ile Ile Ser Val Cys Trp Ser Gly Asp Ile Ile Arg 405 410 415 Ala Ala Phe Ser Ser Ser Gly Leu Asp Gly Leu Gln Val His Ser Asn 420 425 430 Glu Leu Thr Phe Val Glu Ser Val Ser Thr Gly Gly Ile Asp Arg Arg 435 440 445 Val Glu Ser Pro Val Asp Lys Leu Lys Ile Phe Asn Asn Ile Trp Ser 450 455 460 Ser Ser Lys Asp Gln Asp Thr Glu His Ile Ser Ile Tyr Ile Gly Asp 465 470 475 480 Gly Leu Gly Asp Leu Leu Cys Leu Leu Gln Ala Asp Ile Gly Ile Val 485 490 495 Ile Gly Thr Ser Ser Thr Leu Arg Arg Val Gly Lys Arg Phe Gly Val 500 505 510 Ser Phe Val Pro Leu Phe Ser Gly Leu Leu Lys Gln Glu Arg Ala Tyr 515 520 525 Val Glu Gly Ser Ser Cys Trp Thr Lys Gln Ser Gly Ile Leu Tyr Thr 530 535 540 Val Ser Ser Trp Ser Glu Ile His Ala Phe Ile Leu Gly Ser Ser Asn 545 550 555 560 <210> 152 <211> 1641 <212> DNA <213> Physcomitrella patens <220> <221> CDS <222> (1)..(1641) <223> Physcomitrella patens gene encoding TMP phosphatase [XP_001769831] <400> 152 atg aat ttg agc acg caa gct aac aca ggg ttg gcg aag agc ttc tgg 48 Met Asn Leu Ser Thr Gln Ala Asn Thr Gly Leu Ala Lys Ser Phe Trp 1 5 10 15 gct agt tgt aag aga gag gct tat gca tca ctc tac cat ccg ttt gtg 96 Ala Ser Cys Lys Arg Glu Ala Tyr Ala Ser Leu Tyr His Pro Phe Val 20 25 30 gtt gcg tta gcg gct ggc acc ttg cca aaa caa act ttt caa cgt tac 144 Val Ala Leu Ala Ala Gly Thr Leu Pro Lys Gln Thr Phe Gln Arg Tyr 35 40 45 atg gca cag gat gcc tat ttc ttg gag gcg ttc aag aat gcg tat caa 192 Met Ala Gln Asp Ala Tyr Phe Leu Glu Ala Phe Lys Asn Ala Tyr Gln 50 55 60 ctg gct atg gaa acc act aca gac gaa gag gca aag gcc atc att gag 240 Leu Ala Met Glu Thr Thr Thr Asp Glu Glu Ala Lys Ala Ile Ile Glu 65 70 75 80 tcc ctt cag aga gat gtg cag gaa gag ctc aat ttg cac tcg tcg atc 288 Ser Leu Gln Arg Asp Val Gln Glu Glu Leu Asn Leu His Ser Ser Ile 85 90 95 atg cag tct ttg gat gct acc gat cag aat tgc ttt gaa cca aac atg 336 Met Gln Ser Leu Asp Ala Thr Asp Gln Asn Cys Phe Glu Pro Asn Met 100 105 110 gca aca aca gcg tat tgt gat ttt ctg cta gcc aca gct aca gga agt 384 Ala Thr Thr Ala Tyr Cys Asp Phe Leu Leu Ala Thr Ala Thr Gly Ser 115 120 125 aac gaa gca caa aaa ttt gga agc aca agt gct caa atc ata acc gct 432 Asn Glu Ala Gln Lys Phe Gly Ser Thr Ser Ala Gln Ile Ile Thr Ala 130 135 140 atg act cct tgc atg cgg cta tat gca ttt ttg ggg cag gag ctc aaa 480 Met Thr Pro Cys Met Arg Leu Tyr Ala Phe Leu Gly Gln Glu Leu Lys 145 150 155 160 aaa cac gtt gat cat gtt gct gac cat cct tac cag gag tgg att gat 528 Lys His Val Asp His Val Ala Asp His Pro Tyr Gln Glu Trp Ile Asp 165 170 175 act tac tct gct gca gag ttc gag gct gca gct tcg aag att gag cag 576 Thr Tyr Ser Ala Ala Glu Phe Glu Ala Ala Ala Ser Lys Ile Glu Gln 180 185 190 ctg cta gac aag tta act gct act ttg act gga aag cat gaa ata gca 624 Leu Leu Asp Lys Leu Thr Ala Thr Leu Thr Gly Lys His Glu Ile Ala 195 200 205 ttc tta gaa agt ctc tat ctt caa gcc atg aac ttg gag gtg gat ttc 672 Phe Leu Glu Ser Leu Tyr Leu Gln Ala Met Asn Leu Glu Val Asp Phe 210 215 220 ttc ggt gct cag ctg tta ggg cct gtg ctc gta ccc ttc ctc aaa tgc 720 Phe Gly Ala Gln Leu Leu Gly Pro Val Leu Val Pro Phe Leu Lys Cys 225 230 235 240 caa ccg gct cca gag agc tat ata tta ctt gcg tct gac ttt gat tcc 768 Gln Pro Ala Pro Glu Ser Tyr Ile Leu Leu Ala Ser Asp Phe Asp Ser 245 250 255 acg tgc acg ata tct gat tca tgc ccc ata ttg gca gac ctg acc gtg 816 Thr Cys Thr Ile Ser Asp Ser Cys Pro Ile Leu Ala Asp Leu Thr Val 260 265 270 caa act gcg cga aaa tct cac ggt ggt cgt tca gtt ggt gaa tca ggg 864 Gln Thr Ala Arg Lys Ser His Gly Gly Arg Ser Val Gly Glu Ser Gly 275 280 285 gcc agc ttg ttg aaa aaa aga tgg gat gat ctc gtc atg cag tat atg 912 Ala Ser Leu Leu Lys Lys Arg Trp Asp Asp Leu Val Met Gln Tyr Met 290 295 300 gac gag tat gag gac gtt ctg aag cga agc ctg gtg aaa aaa gat aat 960 Asp Glu Tyr Glu Asp Val Leu Lys Arg Ser Leu Val Lys Lys Asp Asn 305 310 315 320 ggc agt gtt aat gcg ctc agt gca gag aat ctc caa gag ttt ctg aag 1008 Gly Ser Val Asn Ala Leu Ser Ala Glu Asn Leu Gln Glu Phe Leu Lys 325 330 335 gaa atg tcc aac ttc gaa cag aag gcc aat gcg agg gtc gaa gag gct 1056 Glu Met Ser Asn Phe Glu Gln Lys Ala Asn Ala Arg Val Glu Glu Ala 340 345 350 gca gtt cta aaa ggc tta tct ctg gct tcg att caa gaa gct gga aaa 1104 Ala Val Leu Lys Gly Leu Ser Leu Ala Ser Ile Gln Glu Ala Gly Lys 355 360 365 tcc atg cct ctt cgt gag ggc tgt tct gac ttt ttt aag cgt ctg gaa 1152 Ser Met Pro Leu Arg Glu Gly Cys Ser Asp Phe Phe Lys Arg Leu Glu 370 375 380 tca gga gag gtt ctt gtt gac aca tgt ata ttg tct gtg tgc tgg agc 1200 Ser Gly Glu Val Leu Val Asp Thr Cys Ile Leu Ser Val Cys Trp Ser 385 390 395 400 aaa acc ttc atc gaa gct gtc ttg gaa aag gtt cgt att cca aac atc 1248 Lys Thr Phe Ile Glu Ala Val Leu Glu Lys Val Arg Ile Pro Asn Ile 405 410 415 aat gcc aac gag ctc gtt ttc gaa gga cgc att tcc acc ggt gct att 1296 Asn Ala Asn Glu Leu Val Phe Glu Gly Arg Ile Ser Thr Gly Ala Ile 420 425 430 atc aaa aac gtc gaa acg gct ctt gac aag caa aga cac ttc gtt cag 1344 Ile Lys Asn Val Glu Thr Ala Leu Asp Lys Gln Arg His Phe Val Gln 435 440 445 ttg ctg gat aat cta aaa cca act caa gac gtg ctg tcc att tat gtt 1392 Leu Leu Asp Asn Leu Lys Pro Thr Gln Asp Val Leu Ser Ile Tyr Val 450 455 460 ggt gat agt ctg act gat ctt ctc tgc cta atc aga gca gac ctg ggt 1440 Gly Asp Ser Leu Thr Asp Leu Leu Cys Leu Ile Arg Ala Asp Leu Gly 465 470 475 480 ata gtt ctc ggt gac agc agc gct ctg aag cag gtg tat ggg cca aaa 1488 Ile Val Leu Gly Asp Ser Ser Ala Leu Lys Gln Val Tyr Gly Pro Lys 485 490 495 atg gcc ccc ctc ttc atg aaa gcc ata ctc ttg gag cag gca aac atg 1536 Met Ala Pro Leu Phe Met Lys Ala Ile Leu Leu Glu Gln Ala Asn Met 500 505 510 cga ggc agg cag caa ccc aca ggt tac gtc ttc act gtc tcc agt tgg 1584 Arg Gly Arg Gln Gln Pro Thr Gly Tyr Val Phe Thr Val Ser Ser Trp 515 520 525 tat gag gtg gaa gcc ttt ctg ttg ggt cct gct aga aac aga cct ttg 1632 Tyr Glu Val Glu Ala Phe Leu Leu Gly Pro Ala Arg Asn Arg Pro Leu 530 535 540 tac atc tag 1641 Tyr Ile 545 <210> 153 <211> 546 <212> PRT <213> Physcomitrella patens <400> 153 Met Asn Leu Ser Thr Gln Ala Asn Thr Gly Leu Ala Lys Ser Phe Trp 1 5 10 15 Ala Ser Cys Lys Arg Glu Ala Tyr Ala Ser Leu Tyr His Pro Phe Val 20 25 30 Val Ala Leu Ala Ala Gly Thr Leu Pro Lys Gln Thr Phe Gln Arg Tyr 35 40 45 Met Ala Gln Asp Ala Tyr Phe Leu Glu Ala Phe Lys Asn Ala Tyr Gln 50 55 60 Leu Ala Met Glu Thr Thr Thr Asp Glu Glu Ala Lys Ala Ile Ile Glu 65 70 75 80 Ser Leu Gln Arg Asp Val Gln Glu Glu Leu Asn Leu His Ser Ser Ile 85 90 95 Met Gln Ser Leu Asp Ala Thr Asp Gln Asn Cys Phe Glu Pro Asn Met 100 105 110 Ala Thr Thr Ala Tyr Cys Asp Phe Leu Leu Ala Thr Ala Thr Gly Ser 115 120 125 Asn Glu Ala Gln Lys Phe Gly Ser Thr Ser Ala Gln Ile Ile Thr Ala 130 135 140 Met Thr Pro Cys Met Arg Leu Tyr Ala Phe Leu Gly Gln Glu Leu Lys 145 150 155 160 Lys His Val Asp His Val Ala Asp His Pro Tyr Gln Glu Trp Ile Asp 165 170 175 Thr Tyr Ser Ala Ala Glu Phe Glu Ala Ala Ala Ser Lys Ile Glu Gln 180 185 190 Leu Leu Asp Lys Leu Thr Ala Thr Leu Thr Gly Lys His Glu Ile Ala 195 200 205 Phe Leu Glu Ser Leu Tyr Leu Gln Ala Met Asn Leu Glu Val Asp Phe 210 215 220 Phe Gly Ala Gln Leu Leu Gly Pro Val Leu Val Pro Phe Leu Lys Cys 225 230 235 240 Gln Pro Ala Pro Glu Ser Tyr Ile Leu Leu Ala Ser Asp Phe Asp Ser 245 250 255 Thr Cys Thr Ile Ser Asp Ser Cys Pro Ile Leu Ala Asp Leu Thr Val 260 265 270 Gln Thr Ala Arg Lys Ser His Gly Gly Arg Ser Val Gly Glu Ser Gly 275 280 285 Ala Ser Leu Leu Lys Lys Arg Trp Asp Asp Leu Val Met Gln Tyr Met 290 295 300 Asp Glu Tyr Glu Asp Val Leu Lys Arg Ser Leu Val Lys Lys Asp Asn 305 310 315 320 Gly Ser Val Asn Ala Leu Ser Ala Glu Asn Leu Gln Glu Phe Leu Lys 325 330 335 Glu Met Ser Asn Phe Glu Gln Lys Ala Asn Ala Arg Val Glu Glu Ala 340 345 350 Ala Val Leu Lys Gly Leu Ser Leu Ala Ser Ile Gln Glu Ala Gly Lys 355 360 365 Ser Met Pro Leu Arg Glu Gly Cys Ser Asp Phe Phe Lys Arg Leu Glu 370 375 380 Ser Gly Glu Val Leu Val Asp Thr Cys Ile Leu Ser Val Cys Trp Ser 385 390 395 400 Lys Thr Phe Ile Glu Ala Val Leu Glu Lys Val Arg Ile Pro Asn Ile 405 410 415 Asn Ala Asn Glu Leu Val Phe Glu Gly Arg Ile Ser Thr Gly Ala Ile 420 425 430 Ile Lys Asn Val Glu Thr Ala Leu Asp Lys Gln Arg His Phe Val Gln 435 440 445 Leu Leu Asp Asn Leu Lys Pro Thr Gln Asp Val Leu Ser Ile Tyr Val 450 455 460 Gly Asp Ser Leu Thr Asp Leu Leu Cys Leu Ile Arg Ala Asp Leu Gly 465 470 475 480 Ile Val Leu Gly Asp Ser Ser Ala Leu Lys Gln Val Tyr Gly Pro Lys 485 490 495 Met Ala Pro Leu Phe Met Lys Ala Ile Leu Leu Glu Gln Ala Asn Met 500 505 510 Arg Gly Arg Gln Gln Pro Thr Gly Tyr Val Phe Thr Val Ser Ser Trp 515 520 525 Tyr Glu Val Glu Ala Phe Leu Leu Gly Pro Ala Arg Asn Arg Pro Leu 530 535 540 Tyr Ile 545 <210> 154 <211> 1593 <212> DNA <213> Selaginella moellendorffii <220> <221> CDS <222> (1)..(1593) <223> Selaginella_moellendorffii gene encoding TMP phosphatase [XP_002990363] <400> 154 atg tcg tgt ttg ctt aga aat gta gtg gcc aga gga ttg agg agc ttg 48 Met Ser Cys Leu Leu Arg Asn Val Val Ala Arg Gly Leu Arg Ser Leu 1 5 10 15 gca agc gcc cag gcg atg gag cca tcc att tca aag cgc ttg tgg cag 96 Ala Ser Ala Gln Ala Met Glu Pro Ser Ile Ser Lys Arg Leu Trp Gln 20 25 30 caa tcc aag cgc gag gca atg gta tgt ctg tat cat cca ttt gtg gtg 144 Gln Ser Lys Arg Glu Ala Met Val Cys Leu Tyr His Pro Phe Val Val 35 40 45 tcc atc gct gct ggg acg ctg gat ctt cac agc ttc cag cga ttc ata 192 Ser Ile Ala Ala Gly Thr Leu Asp Leu His Ser Phe Gln Arg Phe Ile 50 55 60 gcg cag gat tcc ttc ttc ctg acg gca ttc gcg aaa gcc tat ggt ttg 240 Ala Gln Asp Ser Phe Phe Leu Thr Ala Phe Ala Lys Ala Tyr Gly Leu 65 70 75 80 gcc ata gag cgc agc gat gat cga gaa gtt aaa tct gag att tgc aag 288 Ala Ile Glu Arg Ser Asp Asp Arg Glu Val Lys Ser Glu Ile Cys Lys 85 90 95 ctc caa cag gct gtg tac gag gaa ctt gag ctc cat tct tcc ctc atg 336 Leu Gln Gln Ala Val Tyr Glu Glu Leu Glu Leu His Ser Ser Leu Met 100 105 110 aag gct tgg aac ttc gat cat aca cca cca tcg cca gca act tgt gct 384 Lys Ala Trp Asn Phe Asp His Thr Pro Pro Ser Pro Ala Thr Cys Ala 115 120 125 tac aca gat ttt ctc ctc gca gtg gct gct ggg aag aaa att gaa tgc 432 Tyr Thr Asp Phe Leu Leu Ala Val Ala Ala Gly Lys Lys Ile Glu Cys 130 135 140 gag aaa act aag gtg ccg atg ctc gct ctg gca gca atg gct ccg tgc 480 Glu Lys Thr Lys Val Pro Met Leu Ala Leu Ala Ala Met Ala Pro Cys 145 150 155 160 atg cgt ctc tac gct ttc cta ggc caa gag acg aga gtt ttc tct cga 528 Met Arg Leu Tyr Ala Phe Leu Gly Gln Glu Thr Arg Val Phe Ser Arg 165 170 175 gaa aat cat cca tat cgc gac tgg att tcg act tac tcg tcg cct ggt 576 Glu Asn His Pro Tyr Arg Asp Trp Ile Ser Thr Tyr Ser Ser Pro Gly 180 185 190 ttc gag act gct gct act cga ctc gag cag ctt ctc gat agc ctc tcg 624 Phe Glu Thr Ala Ala Thr Arg Leu Glu Gln Leu Leu Asp Ser Leu Ser 195 200 205 gaa gct caa gag act acg gca gcg gaa ttt cag agt atg caa agt ttg 672 Glu Ala Gln Glu Thr Thr Ala Ala Glu Phe Gln Ser Met Gln Ser Leu 210 215 220 tat cac cgt gcc ata gcg tac gag gtg agc ttc ttc gat gcc cag gaa 720 Tyr His Arg Ala Ile Ala Tyr Glu Val Ser Phe Phe Asp Ala Gln Glu 225 230 235 240 gtg cgt ggc agc aac gct ttt gtc ccg ctg cta gag agt gta gca ctc 768 Val Arg Gly Ser Asn Ala Phe Val Pro Leu Leu Glu Ser Val Ala Leu 245 250 255 aag gat cgc aac ttc gtc ctc atc tct gat ttt gat tct act tgc acc 816 Lys Asp Arg Asn Phe Val Leu Ile Ser Asp Phe Asp Ser Thr Cys Thr 260 265 270 gtc tct gat tca tcc cca gtt cta gcg gag ctg gct atg gcg gtc gat 864 Val Ser Asp Ser Ser Pro Val Leu Ala Glu Leu Ala Met Ala Val Asp 275 280 285 cca aat gta agg agg aaa tgg agc agc ctc tcg gac gag tat ttc agg 912 Pro Asn Val Arg Arg Lys Trp Ser Ser Leu Ser Asp Glu Tyr Phe Arg 290 295 300 gac tac tcc aaa ctc ctg gaa gaa gtt gtt ctt cgt gag tac gac tac 960 Asp Tyr Ser Lys Leu Leu Glu Glu Val Val Leu Arg Glu Tyr Asp Tyr 305 310 315 320 gat gcg atc aaa gag gct ctc caa gtt ctt tcc gag ttt gag aag caa 1008 Asp Ala Ile Lys Glu Ala Leu Gln Val Leu Ser Glu Phe Glu Lys Gln 325 330 335 ggg aac gcg aaa atc gac gcc tcc cgc gtt ttg caa ggc att aag atc 1056 Gly Asn Ala Lys Ile Asp Ala Ser Arg Val Leu Gln Gly Ile Lys Ile 340 345 350 gat gat atc aag caa gcc gga caa aac atg gca ctt caa gct ggc tgt 1104 Asp Asp Ile Lys Gln Ala Gly Gln Asn Met Ala Leu Gln Ala Gly Cys 355 360 365 gcc agt gtg ctt tgc agg cta agt tcc aaa atc tct tgt caa atc ctc 1152 Ala Ser Val Leu Cys Arg Leu Ser Ser Lys Ile Ser Cys Gln Ile Leu 370 375 380 tcg gtt tgc tgg agc cgg acc ttc atc gaa gca gct ttc tcc aaa gag 1200 Ser Val Cys Trp Ser Arg Thr Phe Ile Glu Ala Ala Phe Ser Lys Glu 385 390 395 400 aat atc acc aat gtt cct gtc cat tcc aac gaa ctc gaa aac gat ggg 1248 Asn Ile Thr Asn Val Pro Val His Ser Asn Glu Leu Glu Asn Asp Gly 405 410 415 aac ttt aca acc ggg agc ttg atc aga cgc gtc gag aca ccg att gac 1296 Asn Phe Thr Thr Gly Ser Leu Ile Arg Arg Val Glu Thr Pro Ile Asp 420 425 430 aag gaa gag acg atg ttt cgt gag att cta cac gct ccg gac gac aag 1344 Lys Glu Glu Thr Met Phe Arg Glu Ile Leu His Ala Pro Asp Asp Lys 435 440 445 ttt gtg att ttc att gga gac agc ctc acg gat ctg cta gcc ttg ctc 1392 Phe Val Ile Phe Ile Gly Asp Ser Leu Thr Asp Leu Leu Ala Leu Leu 450 455 460 cga gct gac att gga att gtt cta gga acg agc tcc agc ctc gat cga 1440 Arg Ala Asp Ile Gly Ile Val Leu Gly Thr Ser Ser Ser Leu Asp Arg 465 470 475 480 gcc tcc aaa gcc ttt gga gtg aag atc gtg cca ctc ttt tcc ggc ctc 1488 Ala Ser Lys Ala Phe Gly Val Lys Ile Val Pro Leu Phe Ser Gly Leu 485 490 495 gtc cag cgg cag caa agc tct cga tca gcg tgg aga aaa gag gaa gga 1536 Val Gln Arg Gln Gln Ser Ser Arg Ser Ala Trp Arg Lys Glu Glu Gly 500 505 510 gtt ttg tat cga gct tct gga tgg ctg gag ata gaa gcg ttt cta gct 1584 Val Leu Tyr Arg Ala Ser Gly Trp Leu Glu Ile Glu Ala Phe Leu Ala 515 520 525 ggt aat tag 1593 Gly Asn 530 <210> 155 <211> 530 <212> PRT <213> Selaginella moellendorffii <400> 155 Met Ser Cys Leu Leu Arg Asn Val Val Ala Arg Gly Leu Arg Ser Leu 1 5 10 15 Ala Ser Ala Gln Ala Met Glu Pro Ser Ile Ser Lys Arg Leu Trp Gln 20 25 30 Gln Ser Lys Arg Glu Ala Met Val Cys Leu Tyr His Pro Phe Val Val 35 40 45 Ser Ile Ala Ala Gly Thr Leu Asp Leu His Ser Phe Gln Arg Phe Ile 50 55 60 Ala Gln Asp Ser Phe Phe Leu Thr Ala Phe Ala Lys Ala Tyr Gly Leu 65 70 75 80 Ala Ile Glu Arg Ser Asp Asp Arg Glu Val Lys Ser Glu Ile Cys Lys 85 90 95 Leu Gln Gln Ala Val Tyr Glu Glu Leu Glu Leu His Ser Ser Leu Met 100 105 110 Lys Ala Trp Asn Phe Asp His Thr Pro Pro Ser Pro Ala Thr Cys Ala 115 120 125 Tyr Thr Asp Phe Leu Leu Ala Val Ala Ala Gly Lys Lys Ile Glu Cys 130 135 140 Glu Lys Thr Lys Val Pro Met Leu Ala Leu Ala Ala Met Ala Pro Cys 145 150 155 160 Met Arg Leu Tyr Ala Phe Leu Gly Gln Glu Thr Arg Val Phe Ser Arg 165 170 175 Glu Asn His Pro Tyr Arg Asp Trp Ile Ser Thr Tyr Ser Ser Pro Gly 180 185 190 Phe Glu Thr Ala Ala Thr Arg Leu Glu Gln Leu Leu Asp Ser Leu Ser 195 200 205 Glu Ala Gln Glu Thr Thr Ala Ala Glu Phe Gln Ser Met Gln Ser Leu 210 215 220 Tyr His Arg Ala Ile Ala Tyr Glu Val Ser Phe Phe Asp Ala Gln Glu 225 230 235 240 Val Arg Gly Ser Asn Ala Phe Val Pro Leu Leu Glu Ser Val Ala Leu 245 250 255 Lys Asp Arg Asn Phe Val Leu Ile Ser Asp Phe Asp Ser Thr Cys Thr 260 265 270 Val Ser Asp Ser Ser Pro Val Leu Ala Glu Leu Ala Met Ala Val Asp 275 280 285 Pro Asn Val Arg Arg Lys Trp Ser Ser Leu Ser Asp Glu Tyr Phe Arg 290 295 300 Asp Tyr Ser Lys Leu Leu Glu Glu Val Val Leu Arg Glu Tyr Asp Tyr 305 310 315 320 Asp Ala Ile Lys Glu Ala Leu Gln Val Leu Ser Glu Phe Glu Lys Gln 325 330 335 Gly Asn Ala Lys Ile Asp Ala Ser Arg Val Leu Gln Gly Ile Lys Ile 340 345 350 Asp Asp Ile Lys Gln Ala Gly Gln Asn Met Ala Leu Gln Ala Gly Cys 355 360 365 Ala Ser Val Leu Cys Arg Leu Ser Ser Lys Ile Ser Cys Gln Ile Leu 370 375 380 Ser Val Cys Trp Ser Arg Thr Phe Ile Glu Ala Ala Phe Ser Lys Glu 385 390 395 400 Asn Ile Thr Asn Val Pro Val His Ser Asn Glu Leu Glu Asn Asp Gly 405 410 415 Asn Phe Thr Thr Gly Ser Leu Ile Arg Arg Val Glu Thr Pro Ile Asp 420 425 430 Lys Glu Glu Thr Met Phe Arg Glu Ile Leu His Ala Pro Asp Asp Lys 435 440 445 Phe Val Ile Phe Ile Gly Asp Ser Leu Thr Asp Leu Leu Ala Leu Leu 450 455 460 Arg Ala Asp Ile Gly Ile Val Leu Gly Thr Ser Ser Ser Leu Asp Arg 465 470 475 480 Ala Ser Lys Ala Phe Gly Val Lys Ile Val Pro Leu Phe Ser Gly Leu 485 490 495 Val Gln Arg Gln Gln Ser Ser Arg Ser Ala Trp Arg Lys Glu Glu Gly 500 505 510 Val Leu Tyr Arg Ala Ser Gly Trp Leu Glu Ile Glu Ala Phe Leu Ala 515 520 525 Gly Asn 530 <210> 156 <211> 648 <212> DNA <213> Anaerotruncus colihominis <220> <221> CDS <222> (1)..(648) <223> Anaerotruncus colihominis gene encoding TMP phosphatase [WP_006874980] <400> 156 atg atc aaa ggc gcg att ttt gat atg gac ggt acg ctg att gat tcc 48 Met Ile Lys Gly Ala Ile Phe Asp Met Asp Gly Thr Leu Ile Asp Ser 1 5 10 15 atg cct cta tgg gag gac tgc gga cgg gcc ttt tta tcc gcg cgc ggc 96 Met Pro Leu Trp Glu Asp Cys Gly Arg Ala Phe Leu Ser Ala Arg Gly 20 25 30 att act gcg cgt gac gat ctg ggc gaa acg ctc aaa tcc ctg tcg atg 144 Ile Thr Ala Arg Asp Asp Leu Gly Glu Thr Leu Lys Ser Leu Ser Met 35 40 45 gag caa acg gct aat tat ttg cgg gac gca tac ggt att tcc gag aca 192 Glu Gln Thr Ala Asn Tyr Leu Arg Asp Ala Tyr Gly Ile Ser Glu Thr 50 55 60 acc tct gaa atc att gag atg atc aat gga atg gtt act gac gca tat 240 Thr Ser Glu Ile Ile Glu Met Ile Asn Gly Met Val Thr Asp Ala Tyr 65 70 75 80 cag cgc acc atc ccg ctt aaa cgt gac att gcc gcg ttt ctc gag cgc 288 Gln Arg Thr Ile Pro Leu Lys Arg Asp Ile Ala Ala Phe Leu Glu Arg 85 90 95 ctc agg cag gcg gat gtg cgc atg tgt gtc gca acg gca acg gac cgt 336 Leu Arg Gln Ala Asp Val Arg Met Cys Val Ala Thr Ala Thr Asp Arg 100 105 110 cca ctg gtg gag gcg gcg ctt gga cgc ctt gac ctc ctg ccc ttt ttt 384 Pro Leu Val Glu Ala Ala Leu Gly Arg Leu Asp Leu Leu Pro Phe Phe 115 120 125 gaa cgg att ttc acc tgt tcg gag gtg ggg gcc ggc aag gac cgc ccc 432 Glu Arg Ile Phe Thr Cys Ser Glu Val Gly Ala Gly Lys Asp Arg Pro 130 135 140 gat atc ttt gag cag gcg tgc gcc gcg ctt ggc acg ccg cgc ggc gaa 480 Asp Ile Phe Glu Gln Ala Cys Ala Ala Leu Gly Thr Pro Arg Gly Glu 145 150 155 160 acc gtc atc ttt gag gat gct ctt tat gcg att gaa aca gct cgg cgc 528 Thr Val Ile Phe Glu Asp Ala Leu Tyr Ala Ile Glu Thr Ala Arg Arg 165 170 175 gcc ggg ttc cgc gtt gtc gca atc gcg gac gac gcc tcc gcc ggc gac 576 Ala Gly Phe Arg Val Val Ala Ile Ala Asp Asp Ala Ser Ala Gly Asp 180 185 190 gag gcg cgc ata gcc gca ctg tct gag caa tat ata cat aac tat gag 624 Glu Ala Arg Ile Ala Ala Leu Ser Glu Gln Tyr Ile His Asn Tyr Glu 195 200 205 gaa tgc gag gta aac agt tta tga 648 Glu Cys Glu Val Asn Ser Leu 210 215 <210> 157 <211> 215 <212> PRT <213> Anaerotruncus colihominis <400> 157 Met Ile Lys Gly Ala Ile Phe Asp Met Asp Gly Thr Leu Ile Asp Ser 1 5 10 15 Met Pro Leu Trp Glu Asp Cys Gly Arg Ala Phe Leu Ser Ala Arg Gly 20 25 30 Ile Thr Ala Arg Asp Asp Leu Gly Glu Thr Leu Lys Ser Leu Ser Met 35 40 45 Glu Gln Thr Ala Asn Tyr Leu Arg Asp Ala Tyr Gly Ile Ser Glu Thr 50 55 60 Thr Ser Glu Ile Ile Glu Met Ile Asn Gly Met Val Thr Asp Ala Tyr 65 70 75 80 Gln Arg Thr Ile Pro Leu Lys Arg Asp Ile Ala Ala Phe Leu Glu Arg 85 90 95 Leu Arg Gln Ala Asp Val Arg Met Cys Val Ala Thr Ala Thr Asp Arg 100 105 110 Pro Leu Val Glu Ala Ala Leu Gly Arg Leu Asp Leu Leu Pro Phe Phe 115 120 125 Glu Arg Ile Phe Thr Cys Ser Glu Val Gly Ala Gly Lys Asp Arg Pro 130 135 140 Asp Ile Phe Glu Gln Ala Cys Ala Ala Leu Gly Thr Pro Arg Gly Glu 145 150 155 160 Thr Val Ile Phe Glu Asp Ala Leu Tyr Ala Ile Glu Thr Ala Arg Arg 165 170 175 Ala Gly Phe Arg Val Val Ala Ile Ala Asp Asp Ala Ser Ala Gly Asp 180 185 190 Glu Ala Arg Ile Ala Ala Leu Ser Glu Gln Tyr Ile His Asn Tyr Glu 195 200 205 Glu Cys Glu Val Asn Ser Leu 210 215 <210> 158 <211> 666 <212> DNA <213> Eubacterium ventriosum <220> <221> CDS <222> (1)..(666) <223> Eubacterium ventriosum gene encoding TMP phosphatase [WP_005362972] <400> 158 atg tca aca gga ttt ata ttt gat gta gat gga aca ata cta gac tca 48 Met Ser Thr Gly Phe Ile Phe Asp Val Asp Gly Thr Ile Leu Asp Ser 1 5 10 15 atg gga ata tgg atg aac gta gga gaa cta tat cta aaa gat atg gga 96 Met Gly Ile Trp Met Asn Val Gly Glu Leu Tyr Leu Lys Asp Met Gly 20 25 30 ata aag gcg gaa cca aat ctt gga gaa att cta ttc gaa atg aca atg 144 Ile Lys Ala Glu Pro Asn Leu Gly Glu Ile Leu Phe Glu Met Thr Met 35 40 45 aat gaa ggt gca gaa tac ata caa aaa aag tat aat cta aac ctt aca 192 Asn Glu Gly Ala Glu Tyr Ile Gln Lys Lys Tyr Asn Leu Asn Leu Thr 50 55 60 aca gaa gaa ata tgc acc gga ata aac aac cgt gta tac aaa ttc tac 240 Thr Glu Glu Ile Cys Thr Gly Ile Asn Asn Arg Val Tyr Lys Phe Tyr 65 70 75 80 gaa aaa gaa gca atg cca aaa cca aaa gtt atc gac ttt ata gaa caa 288 Glu Lys Glu Ala Met Pro Lys Pro Lys Val Ile Asp Phe Ile Glu Gln 85 90 95 gcc tac gag aac aaa atc cca atg aca ata gca acg tca aca gac aga 336 Ala Tyr Glu Asn Lys Ile Pro Met Thr Ile Ala Thr Ser Thr Asp Arg 100 105 110 cca atg ata gaa gca gct ttc aaa aga ctg cac ata gac aaa tat ttt 384 Pro Met Ile Glu Ala Ala Phe Lys Arg Leu His Ile Asp Lys Tyr Phe 115 120 125 aaa aaa ata ttt acc acg aca gag gtt ggg tat gga aaa gac aaa ccg 432 Lys Lys Ile Phe Thr Thr Thr Glu Val Gly Tyr Gly Lys Asp Lys Pro 130 135 140 gac atc ttc ata aaa gca atg gaa gaa atg gga aca aca cca aag caa 480 Asp Ile Phe Ile Lys Ala Met Glu Glu Met Gly Thr Thr Pro Lys Gln 145 150 155 160 aca tgg cta ttt gaa gat gga gca tac tca ata gaa aca gcc aaa caa 528 Thr Trp Leu Phe Glu Asp Gly Ala Tyr Ser Ile Glu Thr Ala Lys Gln 165 170 175 cta ggc ata aaa aca ata gga atc tac gat cct gca agc gaa aaa gac 576 Leu Gly Ile Lys Thr Ile Gly Ile Tyr Asp Pro Ala Ser Glu Lys Asp 180 185 190 cag gaa aaa ata aga aac cta aca aac atc tac ata aaa aat tgg aca 624 Gln Glu Lys Ile Arg Asn Leu Thr Asn Ile Tyr Ile Lys Asn Trp Thr 195 200 205 gaa cac aaa acc cta ctt aac caa ata caa aac aac aag tag 666 Glu His Lys Thr Leu Leu Asn Gln Ile Gln Asn Asn Lys 210 215 220 <210> 159 <211> 221 <212> PRT <213> Eubacterium ventriosum <400> 159 Met Ser Thr Gly Phe Ile Phe Asp Val Asp Gly Thr Ile Leu Asp Ser 1 5 10 15 Met Gly Ile Trp Met Asn Val Gly Glu Leu Tyr Leu Lys Asp Met Gly 20 25 30 Ile Lys Ala Glu Pro Asn Leu Gly Glu Ile Leu Phe Glu Met Thr Met 35 40 45 Asn Glu Gly Ala Glu Tyr Ile Gln Lys Lys Tyr Asn Leu Asn Leu Thr 50 55 60 Thr Glu Glu Ile Cys Thr Gly Ile Asn Asn Arg Val Tyr Lys Phe Tyr 65 70 75 80 Glu Lys Glu Ala Met Pro Lys Pro Lys Val Ile Asp Phe Ile Glu Gln 85 90 95 Ala Tyr Glu Asn Lys Ile Pro Met Thr Ile Ala Thr Ser Thr Asp Arg 100 105 110 Pro Met Ile Glu Ala Ala Phe Lys Arg Leu His Ile Asp Lys Tyr Phe 115 120 125 Lys Lys Ile Phe Thr Thr Thr Glu Val Gly Tyr Gly Lys Asp Lys Pro 130 135 140 Asp Ile Phe Ile Lys Ala Met Glu Glu Met Gly Thr Thr Pro Lys Gln 145 150 155 160 Thr Trp Leu Phe Glu Asp Gly Ala Tyr Ser Ile Glu Thr Ala Lys Gln 165 170 175 Leu Gly Ile Lys Thr Ile Gly Ile Tyr Asp Pro Ala Ser Glu Lys Asp 180 185 190 Gln Glu Lys Ile Arg Asn Leu Thr Asn Ile Tyr Ile Lys Asn Trp Thr 195 200 205 Glu His Lys Thr Leu Leu Asn Gln Ile Gln Asn Asn Lys 210 215 220 <210> 160 <211> 1482 <212> DNA <213> Coprococcus eutactus <220> <221> CDS <222> (1)..(1482) <223> Coprococcus eutactus ATCC 27759 gene encoding TMP phosphatase [EDP27707] <400> 160 atg aaa aag ata gtt atc agc gat ata aaa ggt gcg ata ttt gac atg 48 Met Lys Lys Ile Val Ile Ser Asp Ile Lys Gly Ala Ile Phe Asp Met 1 5 10 15 gat gga gtt ctg ctg gac tct atg ccg atg tgg gac cat gcg ggc gag 96 Asp Gly Val Leu Leu Asp Ser Met Pro Met Trp Asp His Ala Gly Glu 20 25 30 atg tac ctt gca gga cag ggg ata gag gct gag cct gat ctt gaa aaa 144 Met Tyr Leu Ala Gly Gln Gly Ile Glu Ala Glu Pro Asp Leu Glu Lys 35 40 45 gtc ttg ttt aca atg act atg caa aag ggc gct gaa tat ata cgt gat 192 Val Leu Phe Thr Met Thr Met Gln Lys Gly Ala Glu Tyr Ile Arg Asp 50 55 60 cat tat ggg tta aaa ctc acg gcg gat gag atc ata gat ggc ata aat 240 His Tyr Gly Leu Lys Leu Thr Ala Asp Glu Ile Ile Asp Gly Ile Asn 65 70 75 80 gag act gtg aga gat ttc tat gca aat aag gtt gtg cct aag aat gga 288 Glu Thr Val Arg Asp Phe Tyr Ala Asn Lys Val Val Pro Lys Asn Gly 85 90 95 gtc ctt aag ttc ctc agg ctg ttg aag agt cac aat ata cct gta acc 336 Val Leu Lys Phe Leu Arg Leu Leu Lys Ser His Asn Ile Pro Val Thr 100 105 110 gtt gca act tcg acc gac aga tgc cat gtg gag gct gct ctt tca aga 384 Val Ala Thr Ser Thr Asp Arg Cys His Val Glu Ala Ala Leu Ser Arg 115 120 125 aat gga ctt atg gaa tat gta gac aag ata ttt acg tgt tcg gaa gtt 432 Asn Gly Leu Met Glu Tyr Val Asp Lys Ile Phe Thr Cys Ser Glu Val 130 135 140 ggc gtt gga aag gct gcc tct cca aag ata tat gag ctt gcg gcc gaa 480 Gly Val Gly Lys Ala Ala Ser Pro Lys Ile Tyr Glu Leu Ala Ala Glu 145 150 155 160 ttt atg ggg acg aaa gtc ggc gag tca ttt gtg ttc gag gat gcc tat 528 Phe Met Gly Thr Lys Val Gly Glu Ser Phe Val Phe Glu Asp Ala Tyr 165 170 175 cat gcg gcc gag aca gct cag aat gcg gga ttt aca gtt gta gga ctc 576 His Ala Ala Glu Thr Ala Gln Asn Ala Gly Phe Thr Val Val Gly Leu 180 185 190 tat gac gag tca agc cgt gac atg caa gca gaa ctt aag gtt cac tgc 624 Tyr Asp Glu Ser Ser Arg Asp Met Gln Ala Glu Leu Lys Val His Cys 195 200 205 aat tat tac tat ttg gga ttt gcc gag ctt ata gat gag ctg ctg cct 672 Asn Tyr Tyr Tyr Leu Gly Phe Ala Glu Leu Ile Asp Glu Leu Leu Pro 210 215 220 gac aga agc cag ctt gca ccg gtt ctt acc atc gcg ggc agt gat tca 720 Asp Arg Ser Gln Leu Ala Pro Val Leu Thr Ile Ala Gly Ser Asp Ser 225 230 235 240 tcg gga ggt gcg gga ata cag gca gat ctt aag acc atg cag gca aat 768 Ser Gly Gly Ala Gly Ile Gln Ala Asp Leu Lys Thr Met Gln Ala Asn 245 250 255 gga gtg ttt ggc atg agc gca gta act gcc ttg acg gcg cag aat acc 816 Gly Val Phe Gly Met Ser Ala Val Thr Ala Leu Thr Ala Gln Asn Thr 260 265 270 aca ggt gtg aca tcc atc atg aat gtg aca cct gac ata ctt gca gat 864 Thr Gly Val Thr Ser Ile Met Asn Val Thr Pro Asp Ile Leu Ala Asp 275 280 285 cag ata gat gca gta ttt aca gat ata aga cca cag gcg gtc aag ata 912 Gln Ile Asp Ala Val Phe Thr Asp Ile Arg Pro Gln Ala Val Lys Ile 290 295 300 ggt atg gtg tct gtg cca gaa ctt ata aat gtg atc gca gac aag ctt 960 Gly Met Val Ser Val Pro Glu Leu Ile Asn Val Ile Ala Asp Lys Leu 305 310 315 320 gaa ttt tac agg gcg gag aat gtg gtg ctt gat cct gtg atg gtt gcg 1008 Glu Phe Tyr Arg Ala Glu Asn Val Val Leu Asp Pro Val Met Val Ala 325 330 335 aca agc ggt gct aaa ctc ata agc gat gat gct gtg gac gtt ttg aca 1056 Thr Ser Gly Ala Lys Leu Ile Ser Asp Asp Ala Val Asp Val Leu Thr 340 345 350 gga agg ctg ttc cca ctt gca aag ctg atc acc cca aat att cca gag 1104 Gly Arg Leu Phe Pro Leu Ala Lys Leu Ile Thr Pro Asn Ile Pro Glu 355 360 365 aca gag gcc ctc aca ggt atg agt atc cgg tct aag gaa gat atg gaa 1152 Thr Glu Ala Leu Thr Gly Met Ser Ile Arg Ser Lys Glu Asp Met Glu 370 375 380 agt gca gca agg aaa ata tat gaa aaa tat ggc tgc tca gtt ctt gtg 1200 Ser Ala Ala Arg Lys Ile Tyr Glu Lys Tyr Gly Cys Ser Val Leu Val 385 390 395 400 aag ggc gga cat agc ata aac gat gcg aat gat atg ctg ttt gat gga 1248 Lys Gly Gly His Ser Ile Asn Asp Ala Asn Asp Met Leu Phe Asp Gly 405 410 415 gag aat gta tca tgg ttt tca ggt gag aga ata gaa aat ccg aat acc 1296 Glu Asn Val Ser Trp Phe Ser Gly Glu Arg Ile Glu Asn Pro Asn Thr 420 425 430 cat gga acg ggg tgt aca ctc tca agt gca ata gcc tcc aac ctt gca 1344 His Gly Thr Gly Cys Thr Leu Ser Ser Ala Ile Ala Ser Asn Leu Ala 435 440 445 aag gga tat gat ata gaa act tct gtg cag aga gca aaa gcg tac atc 1392 Lys Gly Tyr Asp Ile Glu Thr Ser Val Gln Arg Ala Lys Ala Tyr Ile 450 455 460 tca gga gcc ctg gct gcg atg ctt gat cta gga aga gga agc ggc ccg 1440 Ser Gly Ala Leu Ala Ala Met Leu Asp Leu Gly Arg Gly Ser Gly Pro 465 470 475 480 tta aac cat ggc ttt gat ata gac agc aga ttc atg ata taa 1482 Leu Asn His Gly Phe Asp Ile Asp Ser Arg Phe Met Ile 485 490 <210> 161 <211> 493 <212> PRT <213> Coprococcus eutactus <400> 161 Met Lys Lys Ile Val Ile Ser Asp Ile Lys Gly Ala Ile Phe Asp Met 1 5 10 15 Asp Gly Val Leu Leu Asp Ser Met Pro Met Trp Asp His Ala Gly Glu 20 25 30 Met Tyr Leu Ala Gly Gln Gly Ile Glu Ala Glu Pro Asp Leu Glu Lys 35 40 45 Val Leu Phe Thr Met Thr Met Gln Lys Gly Ala Glu Tyr Ile Arg Asp 50 55 60 His Tyr Gly Leu Lys Leu Thr Ala Asp Glu Ile Ile Asp Gly Ile Asn 65 70 75 80 Glu Thr Val Arg Asp Phe Tyr Ala Asn Lys Val Val Pro Lys Asn Gly 85 90 95 Val Leu Lys Phe Leu Arg Leu Leu Lys Ser His Asn Ile Pro Val Thr 100 105 110 Val Ala Thr Ser Thr Asp Arg Cys His Val Glu Ala Ala Leu Ser Arg 115 120 125 Asn Gly Leu Met Glu Tyr Val Asp Lys Ile Phe Thr Cys Ser Glu Val 130 135 140 Gly Val Gly Lys Ala Ala Ser Pro Lys Ile Tyr Glu Leu Ala Ala Glu 145 150 155 160 Phe Met Gly Thr Lys Val Gly Glu Ser Phe Val Phe Glu Asp Ala Tyr 165 170 175 His Ala Ala Glu Thr Ala Gln Asn Ala Gly Phe Thr Val Val Gly Leu 180 185 190 Tyr Asp Glu Ser Ser Arg Asp Met Gln Ala Glu Leu Lys Val His Cys 195 200 205 Asn Tyr Tyr Tyr Leu Gly Phe Ala Glu Leu Ile Asp Glu Leu Leu Pro 210 215 220 Asp Arg Ser Gln Leu Ala Pro Val Leu Thr Ile Ala Gly Ser Asp Ser 225 230 235 240 Ser Gly Gly Ala Gly Ile Gln Ala Asp Leu Lys Thr Met Gln Ala Asn 245 250 255 Gly Val Phe Gly Met Ser Ala Val Thr Ala Leu Thr Ala Gln Asn Thr 260 265 270 Thr Gly Val Thr Ser Ile Met Asn Val Thr Pro Asp Ile Leu Ala Asp 275 280 285 Gln Ile Asp Ala Val Phe Thr Asp Ile Arg Pro Gln Ala Val Lys Ile 290 295 300 Gly Met Val Ser Val Pro Glu Leu Ile Asn Val Ile Ala Asp Lys Leu 305 310 315 320 Glu Phe Tyr Arg Ala Glu Asn Val Val Leu Asp Pro Val Met Val Ala 325 330 335 Thr Ser Gly Ala Lys Leu Ile Ser Asp Asp Ala Val Asp Val Leu Thr 340 345 350 Gly Arg Leu Phe Pro Leu Ala Lys Leu Ile Thr Pro Asn Ile Pro Glu 355 360 365 Thr Glu Ala Leu Thr Gly Met Ser Ile Arg Ser Lys Glu Asp Met Glu 370 375 380 Ser Ala Ala Arg Lys Ile Tyr Glu Lys Tyr Gly Cys Ser Val Leu Val 385 390 395 400 Lys Gly Gly His Ser Ile Asn Asp Ala Asn Asp Met Leu Phe Asp Gly 405 410 415 Glu Asn Val Ser Trp Phe Ser Gly Glu Arg Ile Glu Asn Pro Asn Thr 420 425 430 His Gly Thr Gly Cys Thr Leu Ser Ser Ala Ile Ala Ser Asn Leu Ala 435 440 445 Lys Gly Tyr Asp Ile Glu Thr Ser Val Gln Arg Ala Lys Ala Tyr Ile 450 455 460 Ser Gly Ala Leu Ala Ala Met Leu Asp Leu Gly Arg Gly Ser Gly Pro 465 470 475 480 Leu Asn His Gly Phe Asp Ile Asp Ser Arg Phe Met Ile 485 490 <210> 162 <211> 663 <212> DNA <213> Ruminococcus bromii <220> <221> CDS <222> (1)..(663) <223> Ruminococcus bromii L2-63 gene encoding TMP phosphatase [CBL14666] <400> 162 atg att aaa tct gca ata ttt gat gtt gac ggc aca ctt ctc gat tca 48 Met Ile Lys Ser Ala Ile Phe Asp Val Asp Gly Thr Leu Leu Asp Ser 1 5 10 15 atg aag ata tgg gat gat gca gga gag cgt tac ctc tcg tct gtc ggc 96 Met Lys Ile Trp Asp Asp Ala Gly Glu Arg Tyr Leu Ser Ser Val Gly 20 25 30 aaa aca gcc gaa aac gga ctt tcc gaa aag ctc tgt gat atg agt ctg 144 Lys Thr Ala Glu Asn Gly Leu Ser Glu Lys Leu Cys Asp Met Ser Leu 35 40 45 acg gag ggt gcg gag tat atg aaa aag cag tat gct ctt tcc ttt tca 192 Thr Glu Gly Ala Glu Tyr Met Lys Lys Gln Tyr Ala Leu Ser Phe Ser 50 55 60 act gat gaa ata gtt tcg ggt gtg ctg aaa atc att gaa gat ttt tac 240 Thr Asp Glu Ile Val Ser Gly Val Leu Lys Ile Ile Glu Asp Phe Tyr 65 70 75 80 ttt tat gag gtc ggt tta aaa aac gat gca aaa gaa att ttg cag ttt 288 Phe Tyr Glu Val Gly Leu Lys Asn Asp Ala Lys Glu Ile Leu Gln Phe 85 90 95 ttg gaa tcg aac aat atc aaa atg att att gca aca tca agc gac aaa 336 Leu Glu Ser Asn Asn Ile Lys Met Ile Ile Ala Thr Ser Ser Asp Lys 100 105 110 acg cat att aaa aag gca ttt gaa agg ctc ggt att cta aaa tat ttt 384 Thr His Ile Lys Lys Ala Phe Glu Arg Leu Gly Ile Leu Lys Tyr Phe 115 120 125 acg gat att gtg acc tgt tca caa gtc gga aaa ggc aaa aca agc ccc 432 Thr Asp Ile Val Thr Cys Ser Gln Val Gly Lys Gly Lys Thr Ser Pro 130 135 140 gac att tac ctt gtc tgt gca gat aaa ctc gga aca gct ccg agt gaa 480 Asp Ile Tyr Leu Val Cys Ala Asp Lys Leu Gly Thr Ala Pro Ser Glu 145 150 155 160 acg ctt gta ttc gag gac gct gtt ttt gcc gca gaa act gct cac aag 528 Thr Leu Val Phe Glu Asp Ala Val Phe Ala Ala Glu Thr Ala His Lys 165 170 175 gca ggt ttc aaa acg gtg gga gtg tat gac gaa ttg agc agg aat aat 576 Ala Gly Phe Lys Thr Val Gly Val Tyr Asp Glu Leu Ser Arg Asn Asn 180 185 190 aaa aac aga ata aaa gcc gtt tgc gat tac tac gca gac agc ttt gaa 624 Lys Asn Arg Ile Lys Ala Val Cys Asp Tyr Tyr Ala Asp Ser Phe Glu 195 200 205 aaa gcg gca gat tgg ggg cac cac ctt ttg tcg ctg taa 663 Lys Ala Ala Asp Trp Gly His His Leu Leu Ser Leu 210 215 220 <210> 163 <211> 220 <212> PRT <213> Ruminococcus bromii <400> 163 Met Ile Lys Ser Ala Ile Phe Asp Val Asp Gly Thr Leu Leu Asp Ser 1 5 10 15 Met Lys Ile Trp Asp Asp Ala Gly Glu Arg Tyr Leu Ser Ser Val Gly 20 25 30 Lys Thr Ala Glu Asn Gly Leu Ser Glu Lys Leu Cys Asp Met Ser Leu 35 40 45 Thr Glu Gly Ala Glu Tyr Met Lys Lys Gln Tyr Ala Leu Ser Phe Ser 50 55 60 Thr Asp Glu Ile Val Ser Gly Val Leu Lys Ile Ile Glu Asp Phe Tyr 65 70 75 80 Phe Tyr Glu Val Gly Leu Lys Asn Asp Ala Lys Glu Ile Leu Gln Phe 85 90 95 Leu Glu Ser Asn Asn Ile Lys Met Ile Ile Ala Thr Ser Ser Asp Lys 100 105 110 Thr His Ile Lys Lys Ala Phe Glu Arg Leu Gly Ile Leu Lys Tyr Phe 115 120 125 Thr Asp Ile Val Thr Cys Ser Gln Val Gly Lys Gly Lys Thr Ser Pro 130 135 140 Asp Ile Tyr Leu Val Cys Ala Asp Lys Leu Gly Thr Ala Pro Ser Glu 145 150 155 160 Thr Leu Val Phe Glu Asp Ala Val Phe Ala Ala Glu Thr Ala His Lys 165 170 175 Ala Gly Phe Lys Thr Val Gly Val Tyr Asp Glu Leu Ser Arg Asn Asn 180 185 190 Lys Asn Arg Ile Lys Ala Val Cys Asp Tyr Tyr Ala Asp Ser Phe Glu 195 200 205 Lys Ala Ala Asp Trp Gly His His Leu Leu Ser Leu 210 215 220 <210> 164 <211> 1434 <212> DNA <213> Dorea longicatena <220> <221> CDS <222> (1)..(1434) <223> Dorea longicatena DSM13814 gene encoding TMP phosphatase [EDM62146] <400> 164 atg ata aaa gga gca ata ttt gat gta gac gga acc ctt ctg gat tcc 48 Met Ile Lys Gly Ala Ile Phe Asp Val Asp Gly Thr Leu Leu Asp Ser 1 5 10 15 atg gag atc tgg gaa gac gta gga gtc cgt tat ctg aac agt atc ggt 96 Met Glu Ile Trp Glu Asp Val Gly Val Arg Tyr Leu Asn Ser Ile Gly 20 25 30 ata gag gca gag ccg gat ctt ggg acg gtg tta ttt aca atg agc atc 144 Ile Glu Ala Glu Pro Asp Leu Gly Thr Val Leu Phe Thr Met Ser Ile 35 40 45 cag gaa ggt gca gca tat gta aaa gaa cat tat cat ctg tcc cag gag 192 Gln Glu Gly Ala Ala Tyr Val Lys Glu His Tyr His Leu Ser Gln Glu 50 55 60 ccg gaa gaa att gtg cag gga gtt ctg gac atc atc agc aat tat tat 240 Pro Glu Glu Ile Val Gln Gly Val Leu Asp Ile Ile Ser Asn Tyr Tyr 65 70 75 80 aag aaa acc gca cta tta aag agt gga gtg aag gaa ctt ctg gaa aag 288 Lys Lys Thr Ala Leu Leu Lys Ser Gly Val Lys Glu Leu Leu Glu Lys 85 90 95 ctt gat aag cat aat atc cca atg acg gtt gca tca tcc aat aat aaa 336 Leu Asp Lys His Asn Ile Pro Met Thr Val Ala Ser Ser Asn Asn Lys 100 105 110 aaa gag ata gag atg gca ttt gag cgt ctg gga att gca aaa tat ttt 384 Lys Glu Ile Glu Met Ala Phe Glu Arg Leu Gly Ile Ala Lys Tyr Phe 115 120 125 gac cgg atc ttt acc tgt gaa gag gtc ggt gcg gga aag acg aag ccg 432 Asp Arg Ile Phe Thr Cys Glu Glu Val Gly Ala Gly Lys Thr Lys Pro 130 135 140 gat att tat ctg cgg gca gca gaa tat ctc gga acc cgt ccg gag gag 480 Asp Ile Tyr Leu Arg Ala Ala Glu Tyr Leu Gly Thr Arg Pro Glu Glu 145 150 155 160 acg gtt gta ttc gaa gat gtc att cat gca atc cgt act gca aag cag 528 Thr Val Val Phe Glu Asp Val Ile His Ala Ile Arg Thr Ala Lys Gln 165 170 175 gca ggg ttc cag gtt gta gga atc tat gat gaa gca agt aag gat gac 576 Ala Gly Phe Gln Val Val Gly Ile Tyr Asp Glu Ala Ser Lys Asp Asp 180 185 190 cag gaa gag gtt cag aga gaa gta gac tgg tat tgt aga gag tgg gca 624 Gln Glu Glu Val Gln Arg Glu Val Asp Trp Tyr Cys Arg Glu Trp Ala 195 200 205 gaa ctt atg aaa aaa aag aca gca att aca atc gcc gga agt gat tca 672 Glu Leu Met Lys Lys Lys Thr Ala Ile Thr Ile Ala Gly Ser Asp Ser 210 215 220 agt gga ggt gca gga att cag gca gac atc aag acg atg cag gca aac 720 Ser Gly Gly Ala Gly Ile Gln Ala Asp Ile Lys Thr Met Gln Ala Asn 225 230 235 240 gga gtc tac gca atg agt gca atc acc gca ctg aca gcc cag aat aca 768 Gly Val Tyr Ala Met Ser Ala Ile Thr Ala Leu Thr Ala Gln Asn Thr 245 250 255 acc gga gta acc gga atc atg gaa gta tct ccg gaa ttt cta gaa caa 816 Thr Gly Val Thr Gly Ile Met Glu Val Ser Pro Glu Phe Leu Glu Gln 260 265 270 cag ttg gac gca gtt atc aca gac atc cgt ccg gat gca gtg aaa atc 864 Gln Leu Asp Ala Val Ile Thr Asp Ile Arg Pro Asp Ala Val Lys Ile 275 280 285 ggt atg gtg tca tca gaa gag tta ata aaa atg ata tca aag aaa cta 912 Gly Met Val Ser Ser Glu Glu Leu Ile Lys Met Ile Ser Lys Lys Leu 290 295 300 aaa gag tac cat ctg gag aat atc gta gtt gat cca gtg atg gta gca 960 Lys Glu Tyr His Leu Glu Asn Ile Val Val Asp Pro Val Met Val Ala 305 310 315 320 aca agc gga tcc aga ctg atc agt gaa acc gcg att gat aca tta aaa 1008 Thr Ser Gly Ser Arg Leu Ile Ser Glu Thr Ala Ile Asp Thr Leu Lys 325 330 335 aca cag ctg ctg cca atg gca act gtg atc aca ccg aat atc cca gag 1056 Thr Gln Leu Leu Pro Met Ala Thr Val Ile Thr Pro Asn Ile Pro Glu 340 345 350 gca gaa gtt ctt gca gaa atg gag att aga tca gaa gat gat atg gtg 1104 Ala Glu Val Leu Ala Glu Met Glu Ile Arg Ser Glu Asp Asp Met Val 355 360 365 gaa gca gca aag aag att cat gaa atg tat cac tgt gca gtc tta tgc 1152 Glu Ala Ala Lys Lys Ile His Glu Met Tyr His Cys Ala Val Leu Cys 370 375 380 aaa ggc gga cac agc ctg aat gat gcg aat gat ctc cta tac cag gat 1200 Lys Gly Gly His Ser Leu Asn Asp Ala Asn Asp Leu Leu Tyr Gln Asp 385 390 395 400 gga gaa aca aca tgg ttc cac gga aaa aga atc aac aac ccg aac act 1248 Gly Glu Thr Thr Trp Phe His Gly Lys Arg Ile Asn Asn Pro Asn Thr 405 410 415 cac gga acc ggc tgt acc tta tcc agc gca atc gca tcc aat ctg gca 1296 His Gly Thr Gly Cys Thr Leu Ser Ser Ala Ile Ala Ser Asn Leu Ala 420 425 430 aaa gga tat tct ctg gaa gaa tct att cac cgc gcg aaa gag tat atc 1344 Lys Gly Tyr Ser Leu Glu Glu Ser Ile His Arg Ala Lys Glu Tyr Ile 435 440 445 agc ggg gcg ttg gaa gcc atg tta gat ctg gga aaa gga agc gga ccg 1392 Ser Gly Ala Leu Glu Ala Met Leu Asp Leu Gly Lys Gly Ser Gly Pro 450 455 460 atg gat cat ggg ttt gag atg cgg ggg aga ttt tct att taa 1434 Met Asp His Gly Phe Glu Met Arg Gly Arg Phe Ser Ile 465 470 475 <210> 165 <211> 477 <212> PRT <213> Dorea longicatena <400> 165 Met Ile Lys Gly Ala Ile Phe Asp Val Asp Gly Thr Leu Leu Asp Ser 1 5 10 15 Met Glu Ile Trp Glu Asp Val Gly Val Arg Tyr Leu Asn Ser Ile Gly 20 25 30 Ile Glu Ala Glu Pro Asp Leu Gly Thr Val Leu Phe Thr Met Ser Ile 35 40 45 Gln Glu Gly Ala Ala Tyr Val Lys Glu His Tyr His Leu Ser Gln Glu 50 55 60 Pro Glu Glu Ile Val Gln Gly Val Leu Asp Ile Ile Ser Asn Tyr Tyr 65 70 75 80 Lys Lys Thr Ala Leu Leu Lys Ser Gly Val Lys Glu Leu Leu Glu Lys 85 90 95 Leu Asp Lys His Asn Ile Pro Met Thr Val Ala Ser Ser Asn Asn Lys 100 105 110 Lys Glu Ile Glu Met Ala Phe Glu Arg Leu Gly Ile Ala Lys Tyr Phe 115 120 125 Asp Arg Ile Phe Thr Cys Glu Glu Val Gly Ala Gly Lys Thr Lys Pro 130 135 140 Asp Ile Tyr Leu Arg Ala Ala Glu Tyr Leu Gly Thr Arg Pro Glu Glu 145 150 155 160 Thr Val Val Phe Glu Asp Val Ile His Ala Ile Arg Thr Ala Lys Gln 165 170 175 Ala Gly Phe Gln Val Val Gly Ile Tyr Asp Glu Ala Ser Lys Asp Asp 180 185 190 Gln Glu Glu Val Gln Arg Glu Val Asp Trp Tyr Cys Arg Glu Trp Ala 195 200 205 Glu Leu Met Lys Lys Lys Thr Ala Ile Thr Ile Ala Gly Ser Asp Ser 210 215 220 Ser Gly Gly Ala Gly Ile Gln Ala Asp Ile Lys Thr Met Gln Ala Asn 225 230 235 240 Gly Val Tyr Ala Met Ser Ala Ile Thr Ala Leu Thr Ala Gln Asn Thr 245 250 255 Thr Gly Val Thr Gly Ile Met Glu Val Ser Pro Glu Phe Leu Glu Gln 260 265 270 Gln Leu Asp Ala Val Ile Thr Asp Ile Arg Pro Asp Ala Val Lys Ile 275 280 285 Gly Met Val Ser Ser Glu Glu Leu Ile Lys Met Ile Ser Lys Lys Leu 290 295 300 Lys Glu Tyr His Leu Glu Asn Ile Val Val Asp Pro Val Met Val Ala 305 310 315 320 Thr Ser Gly Ser Arg Leu Ile Ser Glu Thr Ala Ile Asp Thr Leu Lys 325 330 335 Thr Gln Leu Leu Pro Met Ala Thr Val Ile Thr Pro Asn Ile Pro Glu 340 345 350 Ala Glu Val Leu Ala Glu Met Glu Ile Arg Ser Glu Asp Asp Met Val 355 360 365 Glu Ala Ala Lys Lys Ile His Glu Met Tyr His Cys Ala Val Leu Cys 370 375 380 Lys Gly Gly His Ser Leu Asn Asp Ala Asn Asp Leu Leu Tyr Gln Asp 385 390 395 400 Gly Glu Thr Thr Trp Phe His Gly Lys Arg Ile Asn Asn Pro Asn Thr 405 410 415 His Gly Thr Gly Cys Thr Leu Ser Ser Ala Ile Ala Ser Asn Leu Ala 420 425 430 Lys Gly Tyr Ser Leu Glu Glu Ser Ile His Arg Ala Lys Glu Tyr Ile 435 440 445 Ser Gly Ala Leu Glu Ala Met Leu Asp Leu Gly Lys Gly Ser Gly Pro 450 455 460 Met Asp His Gly Phe Glu Met Arg Gly Arg Phe Ser Ile 465 470 475 <210> 166 <211> 1305 <212> DNA <213> Lachnospiraceae bacterium <220> <221> CDS <222> (1)..(1305) <223> Lachnospiraceae_bacterium_3_1_57FAA_CT1 gene encoding TMP phosphatase [EPC05128] <400> 166 atg aaa tgt gac aga aag aca atg ctt ctt tat gcg gtg acc gat cgg 48 Met Lys Cys Asp Arg Lys Thr Met Leu Leu Tyr Ala Val Thr Asp Arg 1 5 10 15 gcc tgg aca gga gaa aag aca ctg ctt atg cag gtc gag gaa gcg ctg 96 Ala Trp Thr Gly Glu Lys Thr Leu Leu Met Gln Val Glu Glu Ala Leu 20 25 30 gca gga ggt gtg acc tgt gtc cag ctt cgt gaa aag gat atg cca aag 144 Ala Gly Gly Val Thr Cys Val Gln Leu Arg Glu Lys Asp Met Pro Lys 35 40 45 gag cag ttc ctg gaa gaa gcg gag agt ata aaa aga ctt tgt cat aaa 192 Glu Gln Phe Leu Glu Glu Ala Glu Ser Ile Lys Arg Leu Cys His Lys 50 55 60 tat ggg atc cct ttt ata att gac gat gat gtg gag ctg gcc gta cgc 240 Tyr Gly Ile Pro Phe Ile Ile Asp Asp Asp Val Glu Leu Ala Val Arg 65 70 75 80 tgc ggc gcg gac ggg gtg cat gtg gga cag cat gat atg gag gca ggc 288 Cys Gly Ala Asp Gly Val His Val Gly Gln His Asp Met Glu Ala Gly 85 90 95 gcg gtc cgc cgg aaa atc gga gac ggc atg ctg ctg ggc gta tca gtc 336 Ala Val Arg Arg Lys Ile Gly Asp Gly Met Leu Leu Gly Val Ser Val 100 105 110 cag act gtg gaa cag gca gtg gaa gcc gaa aaa aag gga gcg gat tac 384 Gln Thr Val Glu Gln Ala Val Glu Ala Glu Lys Lys Gly Ala Asp Tyr 115 120 125 ctt ggt gtg ggc gct gtg ttt tcc act tcc acg aaa acg gac gca cag 432 Leu Gly Val Gly Ala Val Phe Ser Thr Ser Thr Lys Thr Asp Ala Gln 130 135 140 gag gtt tcc ctg gat acc ctc cgg gaa atc tgc cgg gcg gtg tcc gta 480 Glu Val Ser Leu Asp Thr Leu Arg Glu Ile Cys Arg Ala Val Ser Val 145 150 155 160 ccc gtc tgt gca atc gga ggg ata cac aaa gga aat atg cat ttg ctg 528 Pro Val Cys Ala Ile Gly Gly Ile His Lys Gly Asn Met His Leu Leu 165 170 175 cag gat acg gga atc gat ggg gtg gct ttg gtg tcg gcc atc ttt tcc 576 Gln Asp Thr Gly Ile Asp Gly Val Ala Leu Val Ser Ala Ile Phe Ser 180 185 190 agt ccc tgc ata cag aag gaa tgc agg gag ctg cgg gtc ctg gca gag 624 Ser Pro Cys Ile Gln Lys Glu Cys Arg Glu Leu Arg Val Leu Ala Glu 195 200 205 aga ctg aaa agg aaa ggg gct att ttt gat gcg gac gga acc ctg ctg 672 Arg Leu Lys Arg Lys Gly Ala Ile Phe Asp Ala Asp Gly Thr Leu Leu 210 215 220 gat tcc atg tcc gtt tgg gat act ctg ggt gaa aaa tat ctg cgg aaa 720 Asp Ser Met Ser Val Trp Asp Thr Leu Gly Glu Lys Tyr Leu Arg Lys 225 230 235 240 aag ggt att gtt ccg gaa aag aac atc agg gaa aca ata aaa aat atg 768 Lys Gly Ile Val Pro Glu Lys Asn Ile Arg Glu Thr Ile Lys Asn Met 245 250 255 agt ctt cct cag gct gcg gtc tat ttt cag act gct tat ggg att gcg 816 Ser Leu Pro Gln Ala Ala Val Tyr Phe Gln Thr Ala Tyr Gly Ile Ala 260 265 270 gat gca gaa gac aag att ata gag gat att aat gga ata gcg gcg tcc 864 Asp Ala Glu Asp Lys Ile Ile Glu Asp Ile Asn Gly Ile Ala Ala Ser 275 280 285 ttt tac atc aat gag gtg aag ctg aag gaa ggc gtg aaa acg gtt ctg 912 Phe Tyr Ile Asn Glu Val Lys Leu Lys Glu Gly Val Lys Thr Val Leu 290 295 300 gac aag ctg aag cag aaa aac gta aag atg tgt gtg gcg acg gct acg 960 Asp Lys Leu Lys Gln Lys Asn Val Lys Met Cys Val Ala Thr Ala Thr 305 310 315 320 gac aaa ggg ctg att gaa aag gca ctt gag aga aac gga atc aga gat 1008 Asp Lys Gly Leu Ile Glu Lys Ala Leu Glu Arg Asn Gly Ile Arg Asp 325 330 335 tat ttt gag gct gtc ctc acc tgc acg gat gtg ggc gcg gga aag gat 1056 Tyr Phe Glu Ala Val Leu Thr Cys Thr Asp Val Gly Ala Gly Lys Asp 340 345 350 gag ccg gtt atc ttc cgt aag gcc ggg cag ctt ctc gga aca gca aaa 1104 Glu Pro Val Ile Phe Arg Lys Ala Gly Gln Leu Leu Gly Thr Ala Lys 355 360 365 gag gat acc att gta att gaa gat gcc ttg tat gct gtt aag aca gcg 1152 Glu Asp Thr Ile Val Ile Glu Asp Ala Leu Tyr Ala Val Lys Thr Ala 370 375 380 aaa gag gac ggt ttc ctg gtg gcg gct gtt tat gat ccg tca gca gaa 1200 Lys Glu Asp Gly Phe Leu Val Ala Ala Val Tyr Asp Pro Ser Ala Glu 385 390 395 400 aag gag gaa ccg gag atc cgg gag atc tct gac ttc tat ttc cgg tca 1248 Lys Glu Glu Pro Glu Ile Arg Glu Ile Ser Asp Phe Tyr Phe Arg Ser 405 410 415 ttt aat gaa atg gag agt tat ctg aat gaa aaa agt tct tac gat agc 1296 Phe Asn Glu Met Glu Ser Tyr Leu Asn Glu Lys Ser Ser Tyr Asp Ser 420 425 430 ggg ctc tga 1305 Gly Leu <210> 167 <211> 434 <212> PRT <213> Lachnospiraceae bacterium <400> 167 Met Lys Cys Asp Arg Lys Thr Met Leu Leu Tyr Ala Val Thr Asp Arg 1 5 10 15 Ala Trp Thr Gly Glu Lys Thr Leu Leu Met Gln Val Glu Glu Ala Leu 20 25 30 Ala Gly Gly Val Thr Cys Val Gln Leu Arg Glu Lys Asp Met Pro Lys 35 40 45 Glu Gln Phe Leu Glu Glu Ala Glu Ser Ile Lys Arg Leu Cys His Lys 50 55 60 Tyr Gly Ile Pro Phe Ile Ile Asp Asp Asp Val Glu Leu Ala Val Arg 65 70 75 80 Cys Gly Ala Asp Gly Val His Val Gly Gln His Asp Met Glu Ala Gly 85 90 95 Ala Val Arg Arg Lys Ile Gly Asp Gly Met Leu Leu Gly Val Ser Val 100 105 110 Gln Thr Val Glu Gln Ala Val Glu Ala Glu Lys Lys Gly Ala Asp Tyr 115 120 125 Leu Gly Val Gly Ala Val Phe Ser Thr Ser Thr Lys Thr Asp Ala Gln 130 135 140 Glu Val Ser Leu Asp Thr Leu Arg Glu Ile Cys Arg Ala Val Ser Val 145 150 155 160 Pro Val Cys Ala Ile Gly Gly Ile His Lys Gly Asn Met His Leu Leu 165 170 175 Gln Asp Thr Gly Ile Asp Gly Val Ala Leu Val Ser Ala Ile Phe Ser 180 185 190 Ser Pro Cys Ile Gln Lys Glu Cys Arg Glu Leu Arg Val Leu Ala Glu 195 200 205 Arg Leu Lys Arg Lys Gly Ala Ile Phe Asp Ala Asp Gly Thr Leu Leu 210 215 220 Asp Ser Met Ser Val Trp Asp Thr Leu Gly Glu Lys Tyr Leu Arg Lys 225 230 235 240 Lys Gly Ile Val Pro Glu Lys Asn Ile Arg Glu Thr Ile Lys Asn Met 245 250 255 Ser Leu Pro Gln Ala Ala Val Tyr Phe Gln Thr Ala Tyr Gly Ile Ala 260 265 270 Asp Ala Glu Asp Lys Ile Ile Glu Asp Ile Asn Gly Ile Ala Ala Ser 275 280 285 Phe Tyr Ile Asn Glu Val Lys Leu Lys Glu Gly Val Lys Thr Val Leu 290 295 300 Asp Lys Leu Lys Gln Lys Asn Val Lys Met Cys Val Ala Thr Ala Thr 305 310 315 320 Asp Lys Gly Leu Ile Glu Lys Ala Leu Glu Arg Asn Gly Ile Arg Asp 325 330 335 Tyr Phe Glu Ala Val Leu Thr Cys Thr Asp Val Gly Ala Gly Lys Asp 340 345 350 Glu Pro Val Ile Phe Arg Lys Ala Gly Gln Leu Leu Gly Thr Ala Lys 355 360 365 Glu Asp Thr Ile Val Ile Glu Asp Ala Leu Tyr Ala Val Lys Thr Ala 370 375 380 Lys Glu Asp Gly Phe Leu Val Ala Ala Val Tyr Asp Pro Ser Ala Glu 385 390 395 400 Lys Glu Glu Pro Glu Ile Arg Glu Ile Ser Asp Phe Tyr Phe Arg Ser 405 410 415 Phe Asn Glu Met Glu Ser Tyr Leu Asn Glu Lys Ser Ser Tyr Asp Ser 420 425 430 Gly Leu <210> 168 <211> 1305 <212> DNA <213> Fusicatenibacter <220> <221> CDS <222> (1)..(1305) <223> Fusicatenibacter gene encoding TMP phosphatase [CUQ30753] <400> 168 atg aaa tgt aac aga aag aca atg ctt ctt tat gcg gtg acc gac cgg 48 Met Lys Cys Asn Arg Lys Thr Met Leu Leu Tyr Ala Val Thr Asp Arg 1 5 10 15 gcc tgg aca gga gaa aag aca ctg ctt acg cag gtc gag gaa gcg ctg 96 Ala Trp Thr Gly Glu Lys Thr Leu Leu Thr Gln Val Glu Glu Ala Leu 20 25 30 gca gga ggt gta acc tgt gtc cag ctt cgt gaa aag gat atg cca aag 144 Ala Gly Gly Val Thr Cys Val Gln Leu Arg Glu Lys Asp Met Pro Lys 35 40 45 gag cag ttc ctg gaa gaa gcg gag agt ata aaa aga ctt tgc cat aaa 192 Glu Gln Phe Leu Glu Glu Ala Glu Ser Ile Lys Arg Leu Cys His Lys 50 55 60 tat ggt gtc cct ttt ata att gac gat gat gtg gag ctg gcc gta cgc 240 Tyr Gly Val Pro Phe Ile Ile Asp Asp Asp Val Glu Leu Ala Val Arg 65 70 75 80 tgc ggt gcg gac ggg gta cat gtg gga cag cat gat atg gag gca ggc 288 Cys Gly Ala Asp Gly Val His Val Gly Gln His Asp Met Glu Ala Gly 85 90 95 gcg gtc cgc cgg aaa atc gga gac ggc atg ctg ctg ggc gta tca gtc 336 Ala Val Arg Arg Lys Ile Gly Asp Gly Met Leu Leu Gly Val Ser Val 100 105 110 cag act gtg gaa cag gca gtg gaa gcc gag aaa aag gga gcg gat tac 384 Gln Thr Val Glu Gln Ala Val Glu Ala Glu Lys Lys Gly Ala Asp Tyr 115 120 125 ctt ggt gtg ggc gct gtg ttt tcc act tcc acg aaa acg gac gca cag 432 Leu Gly Val Gly Ala Val Phe Ser Thr Ser Thr Lys Thr Asp Ala Gln 130 135 140 gag gtt tcc ctg gat acc ctc cgg gaa atc tgc cgg gcg gtg tcc gta 480 Glu Val Ser Leu Asp Thr Leu Arg Glu Ile Cys Arg Ala Val Ser Val 145 150 155 160 ccc gtc tgt gca atc gga ggg ata cac aaa gga aat atg cat ttg ctg 528 Pro Val Cys Ala Ile Gly Gly Ile His Lys Gly Asn Met His Leu Leu 165 170 175 cag gat acg gga atc gat ggg gtg gct ttg gtg tcg gcc atc ttt tcc 576 Gln Asp Thr Gly Ile Asp Gly Val Ala Leu Val Ser Ala Ile Phe Ser 180 185 190 agt ccc tgc ata cag aag gaa tgc agg gag ctg cgg gcc ctg gca gag 624 Ser Pro Cys Ile Gln Lys Glu Cys Arg Glu Leu Arg Ala Leu Ala Glu 195 200 205 agg ctg aaa agg aaa ggg gct att ttt gat gcg gac gga acc ctg ctg 672 Arg Leu Lys Arg Lys Gly Ala Ile Phe Asp Ala Asp Gly Thr Leu Leu 210 215 220 gat tcc atg tct gtt tgg gat acc ctg ggt gaa aaa tat ctg cgg aaa 720 Asp Ser Met Ser Val Trp Asp Thr Leu Gly Glu Lys Tyr Leu Arg Lys 225 230 235 240 aag ggt att gtt ccg gaa aag aac atc agg gaa aca ata aaa aat atg 768 Lys Gly Ile Val Pro Glu Lys Asn Ile Arg Glu Thr Ile Lys Asn Met 245 250 255 agt ctt cct cag gcc gcg gtc tat ttt caa act gct tat ggg atc acg 816 Ser Leu Pro Gln Ala Ala Val Tyr Phe Gln Thr Ala Tyr Gly Ile Thr 260 265 270 gat gca gaa gac aag att ata gag gat att aat gga ata gcg gcg tcc 864 Asp Ala Glu Asp Lys Ile Ile Glu Asp Ile Asn Gly Ile Ala Ala Ser 275 280 285 ttt tac atc aat gag gtg aag ctg aag gaa ggc gtg aaa acg gtt ctg 912 Phe Tyr Ile Asn Glu Val Lys Leu Lys Glu Gly Val Lys Thr Val Leu 290 295 300 gac aag ctg aag cag aaa aac gta aag atg tgt gtg gcg acg gct acg 960 Asp Lys Leu Lys Gln Lys Asn Val Lys Met Cys Val Ala Thr Ala Thr 305 310 315 320 gac aag ggg ctg att gaa aag gca ctt gag aga aac gga atc aga gat 1008 Asp Lys Gly Leu Ile Glu Lys Ala Leu Glu Arg Asn Gly Ile Arg Asp 325 330 335 tat ttt gag gct gtc ctc acc tgc acg gat gtg ggc gcg gga aag gat 1056 Tyr Phe Glu Ala Val Leu Thr Cys Thr Asp Val Gly Ala Gly Lys Asp 340 345 350 gag ccg gtt atc ttc cgt aag gcc ggg cag ctt ctc gga acc gca aaa 1104 Glu Pro Val Ile Phe Arg Lys Ala Gly Gln Leu Leu Gly Thr Ala Lys 355 360 365 gag gat acc att gta att gaa gat gcc ttg tat gct gtt aag aca gcg 1152 Glu Asp Thr Ile Val Ile Glu Asp Ala Leu Tyr Ala Val Lys Thr Ala 370 375 380 aaa gag gac ggt ttc ctg gtg gcg gct gtt tat gat ccg tca gca gaa 1200 Lys Glu Asp Gly Phe Leu Val Ala Ala Val Tyr Asp Pro Ser Ala Glu 385 390 395 400 aag gag gaa ccg gag atc cgg gag atc tct gac ttc tat ttc cgg tca 1248 Lys Glu Glu Pro Glu Ile Arg Glu Ile Ser Asp Phe Tyr Phe Arg Ser 405 410 415 ttt aat gaa atg gag agt tat ctg aat gaa aaa agt tct tac gat agc 1296 Phe Asn Glu Met Glu Ser Tyr Leu Asn Glu Lys Ser Ser Tyr Asp Ser 420 425 430 ggg ctc tga 1305 Gly Leu <210> 169 <211> 434 <212> PRT <213> Fusicatenibacter <400> 169 Met Lys Cys Asn Arg Lys Thr Met Leu Leu Tyr Ala Val Thr Asp Arg 1 5 10 15 Ala Trp Thr Gly Glu Lys Thr Leu Leu Thr Gln Val Glu Glu Ala Leu 20 25 30 Ala Gly Gly Val Thr Cys Val Gln Leu Arg Glu Lys Asp Met Pro Lys 35 40 45 Glu Gln Phe Leu Glu Glu Ala Glu Ser Ile Lys Arg Leu Cys His Lys 50 55 60 Tyr Gly Val Pro Phe Ile Ile Asp Asp Asp Val Glu Leu Ala Val Arg 65 70 75 80 Cys Gly Ala Asp Gly Val His Val Gly Gln His Asp Met Glu Ala Gly 85 90 95 Ala Val Arg Arg Lys Ile Gly Asp Gly Met Leu Leu Gly Val Ser Val 100 105 110 Gln Thr Val Glu Gln Ala Val Glu Ala Glu Lys Lys Gly Ala Asp Tyr 115 120 125 Leu Gly Val Gly Ala Val Phe Ser Thr Ser Thr Lys Thr Asp Ala Gln 130 135 140 Glu Val Ser Leu Asp Thr Leu Arg Glu Ile Cys Arg Ala Val Ser Val 145 150 155 160 Pro Val Cys Ala Ile Gly Gly Ile His Lys Gly Asn Met His Leu Leu 165 170 175 Gln Asp Thr Gly Ile Asp Gly Val Ala Leu Val Ser Ala Ile Phe Ser 180 185 190 Ser Pro Cys Ile Gln Lys Glu Cys Arg Glu Leu Arg Ala Leu Ala Glu 195 200 205 Arg Leu Lys Arg Lys Gly Ala Ile Phe Asp Ala Asp Gly Thr Leu Leu 210 215 220 Asp Ser Met Ser Val Trp Asp Thr Leu Gly Glu Lys Tyr Leu Arg Lys 225 230 235 240 Lys Gly Ile Val Pro Glu Lys Asn Ile Arg Glu Thr Ile Lys Asn Met 245 250 255 Ser Leu Pro Gln Ala Ala Val Tyr Phe Gln Thr Ala Tyr Gly Ile Thr 260 265 270 Asp Ala Glu Asp Lys Ile Ile Glu Asp Ile Asn Gly Ile Ala Ala Ser 275 280 285 Phe Tyr Ile Asn Glu Val Lys Leu Lys Glu Gly Val Lys Thr Val Leu 290 295 300 Asp Lys Leu Lys Gln Lys Asn Val Lys Met Cys Val Ala Thr Ala Thr 305 310 315 320 Asp Lys Gly Leu Ile Glu Lys Ala Leu Glu Arg Asn Gly Ile Arg Asp 325 330 335 Tyr Phe Glu Ala Val Leu Thr Cys Thr Asp Val Gly Ala Gly Lys Asp 340 345 350 Glu Pro Val Ile Phe Arg Lys Ala Gly Gln Leu Leu Gly Thr Ala Lys 355 360 365 Glu Asp Thr Ile Val Ile Glu Asp Ala Leu Tyr Ala Val Lys Thr Ala 370 375 380 Lys Glu Asp Gly Phe Leu Val Ala Ala Val Tyr Asp Pro Ser Ala Glu 385 390 395 400 Lys Glu Glu Pro Glu Ile Arg Glu Ile Ser Asp Phe Tyr Phe Arg Ser 405 410 415 Phe Asn Glu Met Glu Ser Tyr Leu Asn Glu Lys Ser Ser Tyr Asp Ser 420 425 430 Gly Leu <210> 170 <211> 1296 <212> DNA <213> Clostridium species <220> <221> CDS <222> (1)..(1296) <223> Clostridium sp KLE1755 gene encoding TMP phosphatase [ERI68966]: <400> 170 atg aaa tgt gac aga agc atg ctg ctc ctc tat gcc gta acc gac cgt 48 Met Lys Cys Asp Arg Ser Met Leu Leu Leu Tyr Ala Val Thr Asp Arg 1 5 10 15 gcc tgg acg ggt aaa aaa aca ctg ctg cag cag gtg gag gaa gcc ctg 96 Ala Trp Thr Gly Lys Lys Thr Leu Leu Gln Gln Val Glu Glu Ala Leu 20 25 30 gca ggc ggc gcc acc tgc atc cag ctt cgg gaa aag gag ctg ccg gag 144 Ala Gly Gly Ala Thr Cys Ile Gln Leu Arg Glu Lys Glu Leu Pro Glu 35 40 45 gaa gaa ttc cgg cag gaa gcc ctg gct gtg aaa gaa ctt tgc cgc aga 192 Glu Glu Phe Arg Gln Glu Ala Leu Ala Val Lys Glu Leu Cys Arg Arg 50 55 60 tac cat gtc cct ttc ctc att aac gac aac gta gag ctg gct gtc agc 240 Tyr His Val Pro Phe Leu Ile Asn Asp Asn Val Glu Leu Ala Val Ser 65 70 75 80 tgc ggc gcg gac ggc gtc cat gtg ggc cag cac gac atg tct gcg gcg 288 Cys Gly Ala Asp Gly Val His Val Gly Gln His Asp Met Ser Ala Ala 85 90 95 gat gtg cgc cgc aga atc ggc ccc ggc aaa ata ctg gga gta tcc gcg 336 Asp Val Arg Arg Arg Ile Gly Pro Gly Lys Ile Leu Gly Val Ser Ala 100 105 110 cag acg gtg gag cag gcc cgc cag gcg gaa gaa gac ggc gca gat tat 384 Gln Thr Val Glu Gln Ala Arg Gln Ala Glu Glu Asp Gly Ala Asp Tyr 115 120 125 ctg ggc gtg ggc gct gtt ttt tcc acc tcc acc aaa tcc gac gca gac 432 Leu Gly Val Gly Ala Val Phe Ser Thr Ser Thr Lys Ser Asp Ala Asp 130 135 140 gcg gta tcc cat gag acc ctg caa aag atc tgc gcc gca gta tcc atc 480 Ala Val Ser His Glu Thr Leu Gln Lys Ile Cys Ala Ala Val Ser Ile 145 150 155 160 ccc gtc tgc gcc ata ggc ggc atc cat aaa gaa aat ctg cat ttg ctc 528 Pro Val Cys Ala Ile Gly Gly Ile His Lys Glu Asn Leu His Leu Leu 165 170 175 aaa ggc aca ggc atc gcc ggc gtg gcc ctt gtt tcc gcc atc ttc gca 576 Lys Gly Thr Gly Ile Ala Gly Val Ala Leu Val Ser Ala Ile Phe Ala 180 185 190 agc ccg gat atc cgt aag tcc tgc gaa gac ctg aaa aaa ctg gcc ctg 624 Ser Pro Asp Ile Arg Lys Ser Cys Glu Asp Leu Lys Lys Leu Ala Leu 195 200 205 cag ata aac gcg cag gac aca ctg gaa gca ctg ctg cat aca aac atc 672 Gln Ile Asn Ala Gln Asp Thr Leu Glu Ala Leu Leu His Thr Asn Ile 210 215 220 cgc gga gcc atc ttt gac gcg gac ggc acc ctt tta gac tcc atg ggc 720 Arg Gly Ala Ile Phe Asp Ala Asp Gly Thr Leu Leu Asp Ser Met Gly 225 230 235 240 atc tgg gat act ctg ggg gaa gat tac ctg cgt aca aaa ggg aaa atc 768 Ile Trp Asp Thr Leu Gly Glu Asp Tyr Leu Arg Thr Lys Gly Lys Ile 245 250 255 ccc cgg gaa aac ctg cgt gaa acc ttc cgc gac atg agc ctt ctc cag 816 Pro Arg Glu Asn Leu Arg Glu Thr Phe Arg Asp Met Ser Leu Leu Gln 260 265 270 gcc gcc tgc tat tac cgg gaa aat tac gcc ctt acg gaa agc cct gaa 864 Ala Ala Cys Tyr Tyr Arg Glu Asn Tyr Ala Leu Thr Glu Ser Pro Glu 275 280 285 aaa ata gtg gaa gag ctt aac gcc atg atc gcc tcc ttc tat gaa aaa 912 Lys Ile Val Glu Glu Leu Asn Ala Met Ile Ala Ser Phe Tyr Glu Lys 290 295 300 gaa gcc ccc ctg aag gaa gga gcc gcc gcc ttc ctg gaa gcg ctt tgc 960 Glu Ala Pro Leu Lys Glu Gly Ala Ala Ala Phe Leu Glu Ala Leu Cys 305 310 315 320 caa aga aac ata aaa atg tgc att gca aca gcc acc gat cac agc ctt 1008 Gln Arg Asn Ile Lys Met Cys Ile Ala Thr Ala Thr Asp His Ser Leu 325 330 335 atc cgg gcc gcc ctg aag cga tgc gga gtg ctg cat tac ttt act ttt 1056 Ile Arg Ala Ala Leu Lys Arg Cys Gly Val Leu His Tyr Phe Thr Phe 340 345 350 ata ctt acc tgc gga caa gca gga gcg gga aaa gac acc ccc gcc att 1104 Ile Leu Thr Cys Gly Gln Ala Gly Ala Gly Lys Asp Thr Pro Ala Ile 355 360 365 tat gaa gaa gcc ctg gcc ctg ctt gga acc gga aaa aaa gaa acc ttc 1152 Tyr Glu Glu Ala Leu Ala Leu Leu Gly Thr Gly Lys Lys Glu Thr Phe 370 375 380 gtt ttt gaa gat gcc ctg tac gcc ctg aaa acg gcg aaa aca gcc ggc 1200 Val Phe Glu Asp Ala Leu Tyr Ala Leu Lys Thr Ala Lys Thr Ala Gly 385 390 395 400 ttt cct aca gtc ggt gta aaa gac ccc tcc tcc gcc gga cag gaa ggg 1248 Phe Pro Thr Val Gly Val Lys Asp Pro Ser Ser Ala Gly Gln Glu Gly 405 410 415 gag att ata aaa caa gcc gat tac tat ctt tat acc ttc acg aaa tga 1296 Glu Ile Ile Lys Gln Ala Asp Tyr Tyr Leu Tyr Thr Phe Thr Lys 420 425 430 <210> 171 <211> 431 <212> PRT <213> Clostridium species <400> 171 Met Lys Cys Asp Arg Ser Met Leu Leu Leu Tyr Ala Val Thr Asp Arg 1 5 10 15 Ala Trp Thr Gly Lys Lys Thr Leu Leu Gln Gln Val Glu Glu Ala Leu 20 25 30 Ala Gly Gly Ala Thr Cys Ile Gln Leu Arg Glu Lys Glu Leu Pro Glu 35 40 45 Glu Glu Phe Arg Gln Glu Ala Leu Ala Val Lys Glu Leu Cys Arg Arg 50 55 60 Tyr His Val Pro Phe Leu Ile Asn Asp Asn Val Glu Leu Ala Val Ser 65 70 75 80 Cys Gly Ala Asp Gly Val His Val Gly Gln His Asp Met Ser Ala Ala 85 90 95 Asp Val Arg Arg Arg Ile Gly Pro Gly Lys Ile Leu Gly Val Ser Ala 100 105 110 Gln Thr Val Glu Gln Ala Arg Gln Ala Glu Glu Asp Gly Ala Asp Tyr 115 120 125 Leu Gly Val Gly Ala Val Phe Ser Thr Ser Thr Lys Ser Asp Ala Asp 130 135 140 Ala Val Ser His Glu Thr Leu Gln Lys Ile Cys Ala Ala Val Ser Ile 145 150 155 160 Pro Val Cys Ala Ile Gly Gly Ile His Lys Glu Asn Leu His Leu Leu 165 170 175 Lys Gly Thr Gly Ile Ala Gly Val Ala Leu Val Ser Ala Ile Phe Ala 180 185 190 Ser Pro Asp Ile Arg Lys Ser Cys Glu Asp Leu Lys Lys Leu Ala Leu 195 200 205 Gln Ile Asn Ala Gln Asp Thr Leu Glu Ala Leu Leu His Thr Asn Ile 210 215 220 Arg Gly Ala Ile Phe Asp Ala Asp Gly Thr Leu Leu Asp Ser Met Gly 225 230 235 240 Ile Trp Asp Thr Leu Gly Glu Asp Tyr Leu Arg Thr Lys Gly Lys Ile 245 250 255 Pro Arg Glu Asn Leu Arg Glu Thr Phe Arg Asp Met Ser Leu Leu Gln 260 265 270 Ala Ala Cys Tyr Tyr Arg Glu Asn Tyr Ala Leu Thr Glu Ser Pro Glu 275 280 285 Lys Ile Val Glu Glu Leu Asn Ala Met Ile Ala Ser Phe Tyr Glu Lys 290 295 300 Glu Ala Pro Leu Lys Glu Gly Ala Ala Ala Phe Leu Glu Ala Leu Cys 305 310 315 320 Gln Arg Asn Ile Lys Met Cys Ile Ala Thr Ala Thr Asp His Ser Leu 325 330 335 Ile Arg Ala Ala Leu Lys Arg Cys Gly Val Leu His Tyr Phe Thr Phe 340 345 350 Ile Leu Thr Cys Gly Gln Ala Gly Ala Gly Lys Asp Thr Pro Ala Ile 355 360 365 Tyr Glu Glu Ala Leu Ala Leu Leu Gly Thr Gly Lys Lys Glu Thr Phe 370 375 380 Val Phe Glu Asp Ala Leu Tyr Ala Leu Lys Thr Ala Lys Thr Ala Gly 385 390 395 400 Phe Pro Thr Val Gly Val Lys Asp Pro Ser Ser Ala Gly Gln Glu Gly 405 410 415 Glu Ile Ile Lys Gln Ala Asp Tyr Tyr Leu Tyr Thr Phe Thr Lys 420 425 430 <210> 172 <211> 1452 <212> DNA <213> Eubacterium hallii <220> <221> CDS <222> (1)..(1452) <223> Eubacterium hallii gene encoding TMP phosphatase [EEG35494] <400> 172 atg ata aaa gga gca atc ttt gat att gat gga act tta ctt gat tcc 48 Met Ile Lys Gly Ala Ile Phe Asp Ile Asp Gly Thr Leu Leu Asp Ser 1 5 10 15 atg ccc atc tgg gaa aat gca gga gcg aga tat ctt gct act ctt ggc 96 Met Pro Ile Trp Glu Asn Ala Gly Ala Arg Tyr Leu Ala Thr Leu Gly 20 25 30 att aag gca aag cca gat tta aaa gaa cgg ctg gat gct tta tct ttg 144 Ile Lys Ala Lys Pro Asp Leu Lys Glu Arg Leu Asp Ala Leu Ser Leu 35 40 45 cca gaa gga gcc atc tat atg caa aaa gag tat ggc ctt tcg gta tca 192 Pro Glu Gly Ala Ile Tyr Met Gln Lys Glu Tyr Gly Leu Ser Val Ser 50 55 60 gca gaa gac att tta gaa gga gtc aat cag gtt gta aaa gat ttt tac 240 Ala Glu Asp Ile Leu Glu Gly Val Asn Gln Val Val Lys Asp Phe Tyr 65 70 75 80 tat aaa gaa gcg gtc atg aag ccg gga gcc tat gcc tta gta aaa cgt 288 Tyr Lys Glu Ala Val Met Lys Pro Gly Ala Tyr Ala Leu Val Lys Arg 85 90 95 ctg aaa gaa aat ggt gtg aag tta att ata gcc aca gcg aca gat aag 336 Leu Lys Glu Asn Gly Val Lys Leu Ile Ile Ala Thr Ala Thr Asp Lys 100 105 110 gag atg gca aag gcg gcg ctt att cgt aac ggc ata tgg cag gac ttt 384 Glu Met Ala Lys Ala Ala Leu Ile Arg Asn Gly Ile Trp Gln Asp Phe 115 120 125 acg gga atg att acc tgc gag gaa gcc gga gcc gga aag aca agc ccg 432 Thr Gly Met Ile Thr Cys Glu Glu Ala Gly Ala Gly Lys Thr Ser Pro 130 135 140 aag gta ttt gag ctt gca agg caa aag cta ggc act aaa aaa gag gaa 480 Lys Val Phe Glu Leu Ala Arg Gln Lys Leu Gly Thr Lys Lys Glu Glu 145 150 155 160 aca tgg gta ttt gaa gat tct tta tat gcg gtg aaa act gct act gaa 528 Thr Trp Val Phe Glu Asp Ser Leu Tyr Ala Val Lys Thr Ala Thr Glu 165 170 175 gct gga ttt cca gta tgc agt atc tac gat acc tac agt gtg gga aat 576 Ala Gly Phe Pro Val Cys Ser Ile Tyr Asp Thr Tyr Ser Val Gly Asn 180 185 190 gcg aaa gaa atc cag aaa ctt tct aat att tat gtg aga gat ttt tcg 624 Ala Lys Glu Ile Gln Lys Leu Ser Asn Ile Tyr Val Arg Asp Phe Ser 195 200 205 gag ata ggt gat tat tct ttt tca aat atg aaa aca gtt ctt aca att 672 Glu Ile Gly Asp Tyr Ser Phe Ser Asn Met Lys Thr Val Leu Thr Ile 210 215 220 gca ggc agt gat tcg agc gga gga gca ggt att caa gcg gat atc aag 720 Ala Gly Ser Asp Ser Ser Gly Gly Ala Gly Ile Gln Ala Asp Ile Lys 225 230 235 240 act tta act gtt cat aaa gta tat gcc atg act tgt atc acc gca ctt 768 Thr Leu Thr Val His Lys Val Tyr Ala Met Thr Cys Ile Thr Ala Leu 245 250 255 acc gca caa aat aca gtc gga att acc ggg att atg cca gta cca gca 816 Thr Ala Gln Asn Thr Val Gly Ile Thr Gly Ile Met Pro Val Pro Ala 260 265 270 gaa ttt ttt aaa aaa cag atg gaa agc att ttc aca gat ata aag cca 864 Glu Phe Phe Lys Lys Gln Met Glu Ser Ile Phe Thr Asp Ile Lys Pro 275 280 285 gat gcg gtg aaa att gga atg att gct tca aag gaa cag gca gag att 912 Asp Ala Val Lys Ile Gly Met Ile Ala Ser Lys Glu Gln Ala Glu Ile 290 295 300 atc gca gaa tac ctg gaa aaa tat tct atc aaa aat gta gtg gca gac 960 Ile Ala Glu Tyr Leu Glu Lys Tyr Ser Ile Lys Asn Val Val Ala Asp 305 310 315 320 ccg gtg atg att tcg aca agc ggt acg gtt tta gta gaa gaa aca acg 1008 Pro Val Met Ile Ser Thr Ser Gly Thr Val Leu Val Glu Glu Thr Thr 325 330 335 aga aag ata tta tat gag aaa tta tat cca aaa gtt tcc ctg cta acc 1056 Arg Lys Ile Leu Tyr Glu Lys Leu Tyr Pro Lys Val Ser Leu Leu Thr 340 345 350 ccg aac att cca gaa acc gaa ttt tta tcc ggg ata aaa att acc gat 1104 Pro Asn Ile Pro Glu Thr Glu Phe Leu Ser Gly Ile Lys Ile Thr Asp 355 360 365 aaa aaa aca agg gaa gaa gca gca aaa gtc att gca gac agg tgg aat 1152 Lys Lys Thr Arg Glu Glu Ala Ala Lys Val Ile Ala Asp Arg Trp Asn 370 375 380 tgt gcg gtc tta agt aag ggc ggt cac agc gaa gaa aat gcg gac gat 1200 Cys Ala Val Leu Ser Lys Gly Gly His Ser Glu Glu Asn Ala Asp Asp 385 390 395 400 ttg ctt tat gag agt ttt ttg cag gaa gaa aaa aaa gaa aaa gcc gtt 1248 Leu Leu Tyr Glu Ser Phe Leu Gln Glu Glu Lys Lys Glu Lys Ala Val 405 410 415 tgg ttt cca gaa gaa aga att gat aat cca aac aca cac gga acc ggc 1296 Trp Phe Pro Glu Glu Arg Ile Asp Asn Pro Asn Thr His Gly Thr Gly 420 425 430 tgt aca ctt tca agt gcg gta gcg gca aat ctg gca aag gga ttt cct 1344 Cys Thr Leu Ser Ser Ala Val Ala Ala Asn Leu Ala Lys Gly Phe Pro 435 440 445 gta gaa gaa tcc gta aaa aag gca aaa gta tac atc agc gga gca att 1392 Val Glu Glu Ser Val Lys Lys Ala Lys Val Tyr Ile Ser Gly Ala Ile 450 455 460 aga gca atg ctg aat ctt gga cag gga aat ggc ccg cta aat cat atg 1440 Arg Ala Met Leu Asn Leu Gly Gln Gly Asn Gly Pro Leu Asn His Met 465 470 475 480 tgg gat ttg taa 1452 Trp Asp Leu <210> 173 <211> 483 <212> PRT <213> Eubacterium hallii <400> 173 Met Ile Lys Gly Ala Ile Phe Asp Ile Asp Gly Thr Leu Leu Asp Ser 1 5 10 15 Met Pro Ile Trp Glu Asn Ala Gly Ala Arg Tyr Leu Ala Thr Leu Gly 20 25 30 Ile Lys Ala Lys Pro Asp Leu Lys Glu Arg Leu Asp Ala Leu Ser Leu 35 40 45 Pro Glu Gly Ala Ile Tyr Met Gln Lys Glu Tyr Gly Leu Ser Val Ser 50 55 60 Ala Glu Asp Ile Leu Glu Gly Val Asn Gln Val Val Lys Asp Phe Tyr 65 70 75 80 Tyr Lys Glu Ala Val Met Lys Pro Gly Ala Tyr Ala Leu Val Lys Arg 85 90 95 Leu Lys Glu Asn Gly Val Lys Leu Ile Ile Ala Thr Ala Thr Asp Lys 100 105 110 Glu Met Ala Lys Ala Ala Leu Ile Arg Asn Gly Ile Trp Gln Asp Phe 115 120 125 Thr Gly Met Ile Thr Cys Glu Glu Ala Gly Ala Gly Lys Thr Ser Pro 130 135 140 Lys Val Phe Glu Leu Ala Arg Gln Lys Leu Gly Thr Lys Lys Glu Glu 145 150 155 160 Thr Trp Val Phe Glu Asp Ser Leu Tyr Ala Val Lys Thr Ala Thr Glu 165 170 175 Ala Gly Phe Pro Val Cys Ser Ile Tyr Asp Thr Tyr Ser Val Gly Asn 180 185 190 Ala Lys Glu Ile Gln Lys Leu Ser Asn Ile Tyr Val Arg Asp Phe Ser 195 200 205 Glu Ile Gly Asp Tyr Ser Phe Ser Asn Met Lys Thr Val Leu Thr Ile 210 215 220 Ala Gly Ser Asp Ser Ser Gly Gly Ala Gly Ile Gln Ala Asp Ile Lys 225 230 235 240 Thr Leu Thr Val His Lys Val Tyr Ala Met Thr Cys Ile Thr Ala Leu 245 250 255 Thr Ala Gln Asn Thr Val Gly Ile Thr Gly Ile Met Pro Val Pro Ala 260 265 270 Glu Phe Phe Lys Lys Gln Met Glu Ser Ile Phe Thr Asp Ile Lys Pro 275 280 285 Asp Ala Val Lys Ile Gly Met Ile Ala Ser Lys Glu Gln Ala Glu Ile 290 295 300 Ile Ala Glu Tyr Leu Glu Lys Tyr Ser Ile Lys Asn Val Val Ala Asp 305 310 315 320 Pro Val Met Ile Ser Thr Ser Gly Thr Val Leu Val Glu Glu Thr Thr 325 330 335 Arg Lys Ile Leu Tyr Glu Lys Leu Tyr Pro Lys Val Ser Leu Leu Thr 340 345 350 Pro Asn Ile Pro Glu Thr Glu Phe Leu Ser Gly Ile Lys Ile Thr Asp 355 360 365 Lys Lys Thr Arg Glu Glu Ala Ala Lys Val Ile Ala Asp Arg Trp Asn 370 375 380 Cys Ala Val Leu Ser Lys Gly Gly His Ser Glu Glu Asn Ala Asp Asp 385 390 395 400 Leu Leu Tyr Glu Ser Phe Leu Gln Glu Glu Lys Lys Glu Lys Ala Val 405 410 415 Trp Phe Pro Glu Glu Arg Ile Asp Asn Pro Asn Thr His Gly Thr Gly 420 425 430 Cys Thr Leu Ser Ser Ala Val Ala Ala Asn Leu Ala Lys Gly Phe Pro 435 440 445 Val Glu Glu Ser Val Lys Lys Ala Lys Val Tyr Ile Ser Gly Ala Ile 450 455 460 Arg Ala Met Leu Asn Leu Gly Gln Gly Asn Gly Pro Leu Asn His Met 465 470 475 480 Trp Asp Leu <210> 174 <211> 1362 <212> DNA <213> Eubacterium species <220> <221> CDS <222> (1)..(1362) <223> Eubacterium sp. CAG:252 gene encoding TMP phosphatase [CDB67556] <400> 174 atg aaa aat aaa ttt ttc aca cgc gag att tgt gtc tgc gtg cac ttg 48 Met Lys Asn Lys Phe Phe Thr Arg Glu Ile Cys Val Cys Val His Leu 1 5 10 15 aca caa act cgt tat gcg caa aaa acg tgc gca gaa atg agg aat agt 96 Thr Gln Thr Arg Tyr Ala Gln Lys Thr Cys Ala Glu Met Arg Asn Ser 20 25 30 gtg aag gtt aaa gct gag gat atg cag cta tac gct gtt aca gat aca 144 Val Lys Val Lys Ala Glu Asp Met Gln Leu Tyr Ala Val Thr Asp Thr 35 40 45 cag tgg ctt aat gga cgt gac ttt ctt gaa gta ata gaa agc gtt ctt 192 Gln Trp Leu Asn Gly Arg Asp Phe Leu Glu Val Ile Glu Ser Val Leu 50 55 60 gca aat gga gct aca ttt tta cag tta agg gaa aaa aat gcc aca cat 240 Ala Asn Gly Ala Thr Phe Leu Gln Leu Arg Glu Lys Asn Ala Thr His 65 70 75 80 gag gaa ata gtg gca aag gcg aag gca ata aag cca ata gct aag aag 288 Glu Glu Ile Val Ala Lys Ala Lys Ala Ile Lys Pro Ile Ala Lys Lys 85 90 95 tac gga gtg cct ttt gtc ata gat gat gac ata tat gca gct aaa gag 336 Tyr Gly Val Pro Phe Val Ile Asp Asp Asp Ile Tyr Ala Ala Lys Glu 100 105 110 gca gac gtg gat ggt gtc cac ata ggg cag aat gat gca agc tat gag 384 Ala Asp Val Asp Gly Val His Ile Gly Gln Asn Asp Ala Ser Tyr Glu 115 120 125 aag gca aga gaa gtt ctt gga gaa ggc aag ata ata gga atg acg gtc 432 Lys Ala Arg Glu Val Leu Gly Glu Gly Lys Ile Ile Gly Met Thr Val 130 135 140 aag aca agg cag cag gca gaa aat gcc ata aga ctt ggc gct gac tat 480 Lys Thr Arg Gln Gln Ala Glu Asn Ala Ile Arg Leu Gly Ala Asp Tyr 145 150 155 160 gtt gga atg ggg gca gtg ttt cat aca agc act aaa aaa gat gca aag 528 Val Gly Met Gly Ala Val Phe His Thr Ser Thr Lys Lys Asp Ala Lys 165 170 175 gat atg agc agg gaa aca ctt tta gag ctt gca ggg atg atg gag gat 576 Asp Met Ser Arg Glu Thr Leu Leu Glu Leu Ala Gly Met Met Glu Asp 180 185 190 att ccg gtg gtc gcc att ggc ggc ata agc tat gat aac tgc gat tac 624 Ile Pro Val Val Ala Ile Gly Gly Ile Ser Tyr Asp Asn Cys Asp Tyr 195 200 205 tta aag gac aca ggt gtt gat gga ata gca gtt gtt tca gcc ata ttt 672 Leu Lys Asp Thr Gly Val Asp Gly Ile Ala Val Val Ser Ala Ile Phe 210 215 220 gca agt gat gac tgt gcg ctt gcc aca aga aag ctt ttt gta aag aca 720 Ala Ser Asp Asp Cys Ala Leu Ala Thr Arg Lys Leu Phe Val Lys Thr 225 230 235 240 agg gaa ttg ttt gga aag aaa aga aac ata ata atg gat atg gat ggt 768 Arg Glu Leu Phe Gly Lys Lys Arg Asn Ile Ile Met Asp Met Asp Gly 245 250 255 acg ctt gca gac tct atg cct ttc tgg aaa aac agc gca aga gag tat 816 Thr Leu Ala Asp Ser Met Pro Phe Trp Lys Asn Ser Ala Arg Glu Tyr 260 265 270 gcg ata tta cgt gga gca gat att ccg gat aat ttc gat gag ata act 864 Ala Ile Leu Arg Gly Ala Asp Ile Pro Asp Asn Phe Asp Glu Ile Thr 275 280 285 ggc gtt atg gac ctt aat gat tat gct gag tat gtt aaa aat gtt ctt 912 Gly Val Met Asp Leu Asn Asp Tyr Ala Glu Tyr Val Lys Asn Val Leu 290 295 300 ggc ata gat act aat ctt gag cag ata aca gaa gcg gct gtc gag att 960 Gly Ile Asp Thr Asn Leu Glu Gln Ile Thr Glu Ala Ala Val Glu Ile 305 310 315 320 atg aat aaa cat tac gaa aaa gat ata cct gca aag gac ggt atg aca 1008 Met Asn Lys His Tyr Glu Lys Asp Ile Pro Ala Lys Asp Gly Met Thr 325 330 335 gag ctt gtc acg aga gaa tat aag gcc gga agc aga ctt gtt gtg ttt 1056 Glu Leu Val Thr Arg Glu Tyr Lys Ala Gly Ser Arg Leu Val Val Phe 340 345 350 acg gct tca gat aga aga agt gtt gaa att ctt ctt tca cac ctt gga 1104 Thr Ala Ser Asp Arg Arg Ser Val Glu Ile Leu Leu Ser His Leu Gly 355 360 365 ata aga gaa tgt ttt tat gat ata tat aca gtc tat gat gta gga ctt 1152 Ile Arg Glu Cys Phe Tyr Asp Ile Tyr Thr Val Tyr Asp Val Gly Leu 370 375 380 aag aag agt gat aag aac agc tat ctt aag gtg gca gag ctt gca ggc 1200 Lys Lys Ser Asp Lys Asn Ser Tyr Leu Lys Val Ala Glu Leu Ala Gly 385 390 395 400 atg aaa gat aca tca cag gta tgg gta tat gag gat ata tta aga ggt 1248 Met Lys Asp Thr Ser Gln Val Trp Val Tyr Glu Asp Ile Leu Arg Gly 405 410 415 gta aag gca gcg aaa gag gcc gga ctt aat gtg tgt gca gtg tat gat 1296 Val Lys Ala Ala Lys Glu Ala Gly Leu Asn Val Cys Ala Val Tyr Asp 420 425 430 gag gac tcc gca ggc gac tgg gag gac ata aaa gag ctt gcg gat aag 1344 Glu Asp Ser Ala Gly Asp Trp Glu Asp Ile Lys Glu Leu Ala Asp Lys 435 440 445 acc ctt gaa ctt gtg taa 1362 Thr Leu Glu Leu Val 450 <210> 175 <211> 453 <212> PRT <213> Eubacterium species <400> 175 Met Lys Asn Lys Phe Phe Thr Arg Glu Ile Cys Val Cys Val His Leu 1 5 10 15 Thr Gln Thr Arg Tyr Ala Gln Lys Thr Cys Ala Glu Met Arg Asn Ser 20 25 30 Val Lys Val Lys Ala Glu Asp Met Gln Leu Tyr Ala Val Thr Asp Thr 35 40 45 Gln Trp Leu Asn Gly Arg Asp Phe Leu Glu Val Ile Glu Ser Val Leu 50 55 60 Ala Asn Gly Ala Thr Phe Leu Gln Leu Arg Glu Lys Asn Ala Thr His 65 70 75 80 Glu Glu Ile Val Ala Lys Ala Lys Ala Ile Lys Pro Ile Ala Lys Lys 85 90 95 Tyr Gly Val Pro Phe Val Ile Asp Asp Asp Ile Tyr Ala Ala Lys Glu 100 105 110 Ala Asp Val Asp Gly Val His Ile Gly Gln Asn Asp Ala Ser Tyr Glu 115 120 125 Lys Ala Arg Glu Val Leu Gly Glu Gly Lys Ile Ile Gly Met Thr Val 130 135 140 Lys Thr Arg Gln Gln Ala Glu Asn Ala Ile Arg Leu Gly Ala Asp Tyr 145 150 155 160 Val Gly Met Gly Ala Val Phe His Thr Ser Thr Lys Lys Asp Ala Lys 165 170 175 Asp Met Ser Arg Glu Thr Leu Leu Glu Leu Ala Gly Met Met Glu Asp 180 185 190 Ile Pro Val Val Ala Ile Gly Gly Ile Ser Tyr Asp Asn Cys Asp Tyr 195 200 205 Leu Lys Asp Thr Gly Val Asp Gly Ile Ala Val Val Ser Ala Ile Phe 210 215 220 Ala Ser Asp Asp Cys Ala Leu Ala Thr Arg Lys Leu Phe Val Lys Thr 225 230 235 240 Arg Glu Leu Phe Gly Lys Lys Arg Asn Ile Ile Met Asp Met Asp Gly 245 250 255 Thr Leu Ala Asp Ser Met Pro Phe Trp Lys Asn Ser Ala Arg Glu Tyr 260 265 270 Ala Ile Leu Arg Gly Ala Asp Ile Pro Asp Asn Phe Asp Glu Ile Thr 275 280 285 Gly Val Met Asp Leu Asn Asp Tyr Ala Glu Tyr Val Lys Asn Val Leu 290 295 300 Gly Ile Asp Thr Asn Leu Glu Gln Ile Thr Glu Ala Ala Val Glu Ile 305 310 315 320 Met Asn Lys His Tyr Glu Lys Asp Ile Pro Ala Lys Asp Gly Met Thr 325 330 335 Glu Leu Val Thr Arg Glu Tyr Lys Ala Gly Ser Arg Leu Val Val Phe 340 345 350 Thr Ala Ser Asp Arg Arg Ser Val Glu Ile Leu Leu Ser His Leu Gly 355 360 365 Ile Arg Glu Cys Phe Tyr Asp Ile Tyr Thr Val Tyr Asp Val Gly Leu 370 375 380 Lys Lys Ser Asp Lys Asn Ser Tyr Leu Lys Val Ala Glu Leu Ala Gly 385 390 395 400 Met Lys Asp Thr Ser Gln Val Trp Val Tyr Glu Asp Ile Leu Arg Gly 405 410 415 Val Lys Ala Ala Lys Glu Ala Gly Leu Asn Val Cys Ala Val Tyr Asp 420 425 430 Glu Asp Ser Ala Gly Asp Trp Glu Asp Ile Lys Glu Leu Ala Asp Lys 435 440 445 Thr Leu Glu Leu Val 450 <210> 176 <211> 1260 <212> DNA <213> Lachnospiraceae pectinoschiza <220> <221> CDS <222> (1)..(1260) <223> Lachnospiraceae pectinoschiza gene encoding TMP phosphatase [CUQ76318] <400> 176 atg aaa gtt acc cgt gaa gat atg cag ctt tac gcc gtt aca gat acg 48 Met Lys Val Thr Arg Glu Asp Met Gln Leu Tyr Ala Val Thr Asp Thr 1 5 10 15 caa tgg ctt aat ggc agg gat ttc tat gaa gag att gag aaa gtc ctt 96 Gln Trp Leu Asn Gly Arg Asp Phe Tyr Glu Glu Ile Glu Lys Val Leu 20 25 30 gcg gca gga gct aca ttt ttg cag tta aga gaa aag gat tcg aca cac 144 Ala Ala Gly Ala Thr Phe Leu Gln Leu Arg Glu Lys Asp Ser Thr His 35 40 45 gag gag att gta aaa aaa gca ttg gca att aaa ccg ata gca aga aga 192 Glu Glu Ile Val Lys Lys Ala Leu Ala Ile Lys Pro Ile Ala Arg Arg 50 55 60 tat ggt gtg cca ttt gtt ata gat gat gat ata tac gcg gcg tta gag 240 Tyr Gly Val Pro Phe Val Ile Asp Asp Asp Ile Tyr Ala Ala Leu Glu 65 70 75 80 gca gat gtt gac gga gtt cat ata gga caa agt gat gca agc tac gaa 288 Ala Asp Val Asp Gly Val His Ile Gly Gln Ser Asp Ala Ser Tyr Glu 85 90 95 aca gca aga gag ctt cta gga cct gac aag ata ata gga atg aca gta 336 Thr Ala Arg Glu Leu Leu Gly Pro Asp Lys Ile Ile Gly Met Thr Val 100 105 110 aag aca cca gag cag gcg gca aat gcg gca aga ctt ggt gct gat tat 384 Lys Thr Pro Glu Gln Ala Ala Asn Ala Ala Arg Leu Gly Ala Asp Tyr 115 120 125 gtt gga atg gga gct gta ttt cat aca agc acg aag aaa gat gcc aaa 432 Val Gly Met Gly Ala Val Phe His Thr Ser Thr Lys Lys Asp Ala Lys 130 135 140 gat tta agc agg gat aat ctt ctt aag ctt aca gct atg ctt gat atg 480 Asp Leu Ser Arg Asp Asn Leu Leu Lys Leu Thr Ala Met Leu Asp Met 145 150 155 160 ccg ata gtt gca att ggc ggc att aat tat gac aac tgt gat tat tta 528 Pro Ile Val Ala Ile Gly Gly Ile Asn Tyr Asp Asn Cys Asp Tyr Leu 165 170 175 aaa gat aca ggc gtg gac gga att gct gtt gta tcg gcg ata ttt gca 576 Lys Asp Thr Gly Val Asp Gly Ile Ala Val Val Ser Ala Ile Phe Ala 180 185 190 agt gat gac tgc gcg gag gcg aca cga aag ctt tat aag aag aca aga 624 Ser Asp Asp Cys Ala Glu Ala Thr Arg Lys Leu Tyr Lys Lys Thr Arg 195 200 205 aag ctg ttt aat tat aat aag aac ata ata ttt gat atg gac gga aca 672 Lys Leu Phe Asn Tyr Asn Lys Asn Ile Ile Phe Asp Met Asp Gly Thr 210 215 220 ctt gtt gac tct atg ccg ttc tgg aag aat agt gca agg gaa tat gcc 720 Leu Val Asp Ser Met Pro Phe Trp Lys Asn Ser Ala Arg Glu Tyr Ala 225 230 235 240 att tta aga ggt gct aag ctt cca aag aat ttt gat gag ata aca gga 768 Ile Leu Arg Gly Ala Lys Leu Pro Lys Asn Phe Asp Glu Ile Thr Gly 245 250 255 gtt atg gac ctt tcg gaa tat gcg gct tat ctg caa aat gtt ctt ggg 816 Val Met Asp Leu Ser Glu Tyr Ala Ala Tyr Leu Gln Asn Val Leu Gly 260 265 270 att gat aca tcg cta gaa cag ata aca gag gca gca gtt gat att atg 864 Ile Asp Thr Ser Leu Glu Gln Ile Thr Glu Ala Ala Val Asp Ile Met 275 280 285 aat aag cat tat gca agt gat att cct gca aag aag gga atg ata aag 912 Asn Lys His Tyr Ala Ser Asp Ile Pro Ala Lys Lys Gly Met Ile Lys 290 295 300 ctt ata aga aga gaa tat gag gct gga agc aag ctt gta ata ttc agt 960 Leu Ile Arg Arg Glu Tyr Glu Ala Gly Ser Lys Leu Val Ile Phe Ser 305 310 315 320 gct tcc gat act tcc agt gtg gaa att ctt ctt aaa agg tta gaa ata 1008 Ala Ser Asp Thr Ser Ser Val Glu Ile Leu Leu Lys Arg Leu Glu Ile 325 330 335 tat gaa tgt ttt gag gga ata tac aca gta tat gat gtc ggc ata gga 1056 Tyr Glu Cys Phe Glu Gly Ile Tyr Thr Val Tyr Asp Val Gly Ile Gly 340 345 350 aag agt gat aag gaa agc tat aaa aag gtt gcc agg tca gca gga atg 1104 Lys Ser Asp Lys Glu Ser Tyr Lys Lys Val Ala Arg Ser Ala Gly Met 355 360 365 gat ata tct gat acg tgg gtg tat gag gat att cta aga ggc gtt cgg 1152 Asp Ile Ser Asp Thr Trp Val Tyr Glu Asp Ile Leu Arg Gly Val Arg 370 375 380 gcg gca cat aat gct gga ttg aaa gtg tgt gcg gta tat gat aaa gac 1200 Ala Ala His Asn Ala Gly Leu Lys Val Cys Ala Val Tyr Asp Lys Asp 385 390 395 400 tcg gca gat gac tgg gat gag ata tgc agt att gca gat aaa tgt ata 1248 Ser Ala Asp Asp Trp Asp Glu Ile Cys Ser Ile Ala Asp Lys Cys Ile 405 410 415 ata acc gga taa 1260 Ile Thr Gly <210> 177 <211> 419 <212> PRT <213> Lachnospiraceae pectinoschiza <400> 177 Met Lys Val Thr Arg Glu Asp Met Gln Leu Tyr Ala Val Thr Asp Thr 1 5 10 15 Gln Trp Leu Asn Gly Arg Asp Phe Tyr Glu Glu Ile Glu Lys Val Leu 20 25 30 Ala Ala Gly Ala Thr Phe Leu Gln Leu Arg Glu Lys Asp Ser Thr His 35 40 45 Glu Glu Ile Val Lys Lys Ala Leu Ala Ile Lys Pro Ile Ala Arg Arg 50 55 60 Tyr Gly Val Pro Phe Val Ile Asp Asp Asp Ile Tyr Ala Ala Leu Glu 65 70 75 80 Ala Asp Val Asp Gly Val His Ile Gly Gln Ser Asp Ala Ser Tyr Glu 85 90 95 Thr Ala Arg Glu Leu Leu Gly Pro Asp Lys Ile Ile Gly Met Thr Val 100 105 110 Lys Thr Pro Glu Gln Ala Ala Asn Ala Ala Arg Leu Gly Ala Asp Tyr 115 120 125 Val Gly Met Gly Ala Val Phe His Thr Ser Thr Lys Lys Asp Ala Lys 130 135 140 Asp Leu Ser Arg Asp Asn Leu Leu Lys Leu Thr Ala Met Leu Asp Met 145 150 155 160 Pro Ile Val Ala Ile Gly Gly Ile Asn Tyr Asp Asn Cys Asp Tyr Leu 165 170 175 Lys Asp Thr Gly Val Asp Gly Ile Ala Val Val Ser Ala Ile Phe Ala 180 185 190 Ser Asp Asp Cys Ala Glu Ala Thr Arg Lys Leu Tyr Lys Lys Thr Arg 195 200 205 Lys Leu Phe Asn Tyr Asn Lys Asn Ile Ile Phe Asp Met Asp Gly Thr 210 215 220 Leu Val Asp Ser Met Pro Phe Trp Lys Asn Ser Ala Arg Glu Tyr Ala 225 230 235 240 Ile Leu Arg Gly Ala Lys Leu Pro Lys Asn Phe Asp Glu Ile Thr Gly 245 250 255 Val Met Asp Leu Ser Glu Tyr Ala Ala Tyr Leu Gln Asn Val Leu Gly 260 265 270 Ile Asp Thr Ser Leu Glu Gln Ile Thr Glu Ala Ala Val Asp Ile Met 275 280 285 Asn Lys His Tyr Ala Ser Asp Ile Pro Ala Lys Lys Gly Met Ile Lys 290 295 300 Leu Ile Arg Arg Glu Tyr Glu Ala Gly Ser Lys Leu Val Ile Phe Ser 305 310 315 320 Ala Ser Asp Thr Ser Ser Val Glu Ile Leu Leu Lys Arg Leu Glu Ile 325 330 335 Tyr Glu Cys Phe Glu Gly Ile Tyr Thr Val Tyr Asp Val Gly Ile Gly 340 345 350 Lys Ser Asp Lys Glu Ser Tyr Lys Lys Val Ala Arg Ser Ala Gly Met 355 360 365 Asp Ile Ser Asp Thr Trp Val Tyr Glu Asp Ile Leu Arg Gly Val Arg 370 375 380 Ala Ala His Asn Ala Gly Leu Lys Val Cys Ala Val Tyr Asp Lys Asp 385 390 395 400 Ser Ala Asp Asp Trp Asp Glu Ile Cys Ser Ile Ala Asp Lys Cys Ile 405 410 415 Ile Thr Gly <210> 178 <211> 1296 <212> DNA <213> Peptostreptococcaceae bacterium <220> <221> CDS <222> (1)..(1296) <223> Peptostreptococcaceae bacterium OBRC8 gene encoding TMP phosphatase[WP_009530263] <400> 178 atg aaa aat att gac tat aca atg tat tac gtc acc gat gaa gac ctt 48 Met Lys Asn Ile Asp Tyr Thr Met Tyr Tyr Val Thr Asp Glu Asp Leu 1 5 10 15 ttg agc agt aat cat acc ttg gaa aca tct gta caa gat gcc att tta 96 Leu Ser Ser Asn His Thr Leu Glu Thr Ser Val Gln Asp Ala Ile Leu 20 25 30 ggt ggc tgt aca atg ata cag ctt cga gaa aaa cat tca tcc act ctc 144 Gly Gly Cys Thr Met Ile Gln Leu Arg Glu Lys His Ser Ser Thr Leu 35 40 45 gat ttt tat aac aaa gcc ata aaa att aaa gcc att tgc gac aag tac 192 Asp Phe Tyr Asn Lys Ala Ile Lys Ile Lys Ala Ile Cys Asp Lys Tyr 50 55 60 aac ata cct ctt ata ata aat gac aga ata gat gta gct ctt gca ata 240 Asn Ile Pro Leu Ile Ile Asn Asp Arg Ile Asp Val Ala Leu Ala Ile 65 70 75 80 aac gca gac gga gta cat ctc gga caa gac gat atg cct ctt gat att 288 Asn Ala Asp Gly Val His Leu Gly Gln Asp Asp Met Pro Leu Asp Ile 85 90 95 gca aga aaa att atg gga gat ggc aaa att ata gga ata tca act gca 336 Ala Arg Lys Ile Met Gly Asp Gly Lys Ile Ile Gly Ile Ser Thr Ala 100 105 110 act tta gat gaa gct cta atc gct caa caa ggc ggt gca gat tat gta 384 Thr Leu Asp Glu Ala Leu Ile Ala Gln Gln Gly Gly Ala Asp Tyr Val 115 120 125 gga gta ggt gct atg tac agc aca aac aca aaa acc gat gcc aat ttg 432 Gly Val Gly Ala Met Tyr Ser Thr Asn Thr Lys Thr Asp Ala Asn Leu 130 135 140 aca act ata aac gag ctt aca aaa ata aaa aac aat cta aaa ata cct 480 Thr Thr Ile Asn Glu Leu Thr Lys Ile Lys Asn Asn Leu Lys Ile Pro 145 150 155 160 gta gtt gca atc ggc ggt ata aac ctt gac aca ata cct gct cta aaa 528 Val Val Ala Ile Gly Gly Ile Asn Leu Asp Thr Ile Pro Ala Leu Lys 165 170 175 cct gca caa ata gac gga gtt gca ata gta tcc gct ata tct atg cag 576 Pro Ala Gln Ile Asp Gly Val Ala Ile Val Ser Ala Ile Ser Met Gln 180 185 190 gaa gat acc gta tct gca aca aga aaa tta aaa aat act ttt ttg aaa 624 Glu Asp Thr Val Ser Ala Thr Arg Lys Leu Lys Asn Thr Phe Leu Lys 195 200 205 caa tat caa act aaa ggc gta ata ttc gat att gac ggt act ctg ctt 672 Gln Tyr Gln Thr Lys Gly Val Ile Phe Asp Ile Asp Gly Thr Leu Leu 210 215 220 gaa act atg aac ata tgg gac aat gta ctt cta aac ctt atg aat aca 720 Glu Thr Met Asn Ile Trp Asp Asn Val Leu Leu Asn Leu Met Asn Thr 225 230 235 240 ctt aat atc agc tat acc gaa gat gaa ata caa aaa ata tgg aat atg 768 Leu Asn Ile Ser Tyr Thr Glu Asp Glu Ile Gln Lys Ile Trp Asn Met 245 250 255 ggt ttt gca gag ctt gca cag ttc agc ata aaa aaa ttc aag ctt gat 816 Gly Phe Ala Glu Leu Ala Gln Phe Ser Ile Lys Lys Phe Lys Leu Asp 260 265 270 atg agt gta aaa gaa ttt tgg caa ctt ata aaa aaa tta tca gtc gaa 864 Met Ser Val Lys Glu Phe Trp Gln Leu Ile Lys Lys Leu Ser Val Glu 275 280 285 gag tat aaa aat agc aaa ata cac tta aaa aaa ggt gca aaa aaa ctg 912 Glu Tyr Lys Asn Ser Lys Ile His Leu Lys Lys Gly Ala Lys Lys Leu 290 295 300 ctt gag tat ctc aaa gaa aaa ggt gta aaa tta gcc ata gca act gcc 960 Leu Glu Tyr Leu Lys Glu Lys Gly Val Lys Leu Ala Ile Ala Thr Ala 305 310 315 320 ctt tgc aaa gaa cag tat gaa ata gtg ctt aca aag aca ggt atc ata 1008 Leu Cys Lys Glu Gln Tyr Glu Ile Val Leu Thr Lys Thr Gly Ile Ile 325 330 335 gac tat ttt gac ata ata gca tca agc gta gat tta aaa atg gaa aaa 1056 Asp Tyr Phe Asp Ile Ile Ala Ser Ser Val Asp Leu Lys Met Glu Lys 340 345 350 tca gac aga caa ata ttt gac tat ata gca aaa aat cta caa gtt cca 1104 Ser Asp Arg Gln Ile Phe Asp Tyr Ile Ala Lys Asn Leu Gln Val Pro 355 360 365 aac aaa aat ctt att ttc ttt gaa gac gac ata aac tcg tca aca ggt 1152 Asn Lys Asn Leu Ile Phe Phe Glu Asp Asp Ile Asn Ser Ser Thr Gly 370 375 380 gcc aag ttg gca gga cta aaa ctg tgc att gta tca aac aag aaa tat 1200 Ala Lys Leu Ala Gly Leu Lys Leu Cys Ile Val Ser Asn Lys Lys Tyr 385 390 395 400 aac ggt aac agc aaa ttt gac gct ctc ata gat tat aaa ata gat gat 1248 Asn Gly Asn Ser Lys Phe Asp Ala Leu Ile Asp Tyr Lys Ile Asp Asp 405 410 415 ttt gaa aat aaa ttg ata tat gat gaa ata ata gtg gag aaa aat tag 1296 Phe Glu Asn Lys Leu Ile Tyr Asp Glu Ile Ile Val Glu Lys Asn 420 425 430 <210> 179 <211> 431 <212> PRT <213> Peptostreptococcaceae bacterium <400> 179 Met Lys Asn Ile Asp Tyr Thr Met Tyr Tyr Val Thr Asp Glu Asp Leu 1 5 10 15 Leu Ser Ser Asn His Thr Leu Glu Thr Ser Val Gln Asp Ala Ile Leu 20 25 30 Gly Gly Cys Thr Met Ile Gln Leu Arg Glu Lys His Ser Ser Thr Leu 35 40 45 Asp Phe Tyr Asn Lys Ala Ile Lys Ile Lys Ala Ile Cys Asp Lys Tyr 50 55 60 Asn Ile Pro Leu Ile Ile Asn Asp Arg Ile Asp Val Ala Leu Ala Ile 65 70 75 80 Asn Ala Asp Gly Val His Leu Gly Gln Asp Asp Met Pro Leu Asp Ile 85 90 95 Ala Arg Lys Ile Met Gly Asp Gly Lys Ile Ile Gly Ile Ser Thr Ala 100 105 110 Thr Leu Asp Glu Ala Leu Ile Ala Gln Gln Gly Gly Ala Asp Tyr Val 115 120 125 Gly Val Gly Ala Met Tyr Ser Thr Asn Thr Lys Thr Asp Ala Asn Leu 130 135 140 Thr Thr Ile Asn Glu Leu Thr Lys Ile Lys Asn Asn Leu Lys Ile Pro 145 150 155 160 Val Val Ala Ile Gly Gly Ile Asn Leu Asp Thr Ile Pro Ala Leu Lys 165 170 175 Pro Ala Gln Ile Asp Gly Val Ala Ile Val Ser Ala Ile Ser Met Gln 180 185 190 Glu Asp Thr Val Ser Ala Thr Arg Lys Leu Lys Asn Thr Phe Leu Lys 195 200 205 Gln Tyr Gln Thr Lys Gly Val Ile Phe Asp Ile Asp Gly Thr Leu Leu 210 215 220 Glu Thr Met Asn Ile Trp Asp Asn Val Leu Leu Asn Leu Met Asn Thr 225 230 235 240 Leu Asn Ile Ser Tyr Thr Glu Asp Glu Ile Gln Lys Ile Trp Asn Met 245 250 255 Gly Phe Ala Glu Leu Ala Gln Phe Ser Ile Lys Lys Phe Lys Leu Asp 260 265 270 Met Ser Val Lys Glu Phe Trp Gln Leu Ile Lys Lys Leu Ser Val Glu 275 280 285 Glu Tyr Lys Asn Ser Lys Ile His Leu Lys Lys Gly Ala Lys Lys Leu 290 295 300 Leu Glu Tyr Leu Lys Glu Lys Gly Val Lys Leu Ala Ile Ala Thr Ala 305 310 315 320 Leu Cys Lys Glu Gln Tyr Glu Ile Val Leu Thr Lys Thr Gly Ile Ile 325 330 335 Asp Tyr Phe Asp Ile Ile Ala Ser Ser Val Asp Leu Lys Met Glu Lys 340 345 350 Ser Asp Arg Gln Ile Phe Asp Tyr Ile Ala Lys Asn Leu Gln Val Pro 355 360 365 Asn Lys Asn Leu Ile Phe Phe Glu Asp Asp Ile Asn Ser Ser Thr Gly 370 375 380 Ala Lys Leu Ala Gly Leu Lys Leu Cys Ile Val Ser Asn Lys Lys Tyr 385 390 395 400 Asn Gly Asn Ser Lys Phe Asp Ala Leu Ile Asp Tyr Lys Ile Asp Asp 405 410 415 Phe Glu Asn Lys Leu Ile Tyr Asp Glu Ile Ile Val Glu Lys Asn 420 425 430 <210> 180 <211> 1296 <212> DNA <213> Peptostreptococcaceae bacterium <220> <221> CDS <222> (1)..(1296) <223> Peptostreptococcaceae bacterium CM2 gene encoding TMP phosphatase[WP_009527854] <400> 180 atg aaa aat att gac tat aca atg tat tac gtc acc gat gaa gac ctt 48 Met Lys Asn Ile Asp Tyr Thr Met Tyr Tyr Val Thr Asp Glu Asp Leu 1 5 10 15 ttg agc agt aat cac acc ttg gaa aca tct gtg caa gat gcc att tta 96 Leu Ser Ser Asn His Thr Leu Glu Thr Ser Val Gln Asp Ala Ile Leu 20 25 30 ggt ggc tgt aca atg ata cag ctt cga gaa aaa cat tca tcc act ctc 144 Gly Gly Cys Thr Met Ile Gln Leu Arg Glu Lys His Ser Ser Thr Leu 35 40 45 gat ttt tat aac aaa gcc gta aaa att aaa gct att tgc gac aag tac 192 Asp Phe Tyr Asn Lys Ala Val Lys Ile Lys Ala Ile Cys Asp Lys Tyr 50 55 60 aac ata cct ctt ata ata aat gac aga ata gac gta gct ctt gca ata 240 Asn Ile Pro Leu Ile Ile Asn Asp Arg Ile Asp Val Ala Leu Ala Ile 65 70 75 80 aat gca gac gga gta cat ctc gga caa gac gat atg cct ctt gat att 288 Asn Ala Asp Gly Val His Leu Gly Gln Asp Asp Met Pro Leu Asp Ile 85 90 95 gca aga aaa att atg gga gat ggc aaa att ata gga ata tca acc tca 336 Ala Arg Lys Ile Met Gly Asp Gly Lys Ile Ile Gly Ile Ser Thr Ser 100 105 110 act tta gat gaa gct cta atc gct caa caa ggc ggt gca gat tat gta 384 Thr Leu Asp Glu Ala Leu Ile Ala Gln Gln Gly Gly Ala Asp Tyr Val 115 120 125 ggt gta ggt gct atg tac agc aca aac aca aaa act gat gcc aat ttg 432 Gly Val Gly Ala Met Tyr Ser Thr Asn Thr Lys Thr Asp Ala Asn Leu 130 135 140 aca act ata gac gag ctt aca aaa ata aaa aac aat tta aaa ata cct 480 Thr Thr Ile Asp Glu Leu Thr Lys Ile Lys Asn Asn Leu Lys Ile Pro 145 150 155 160 gtt gtt gca atc ggc ggt ata aac ctt gac act ata ccc gct cta aaa 528 Val Val Ala Ile Gly Gly Ile Asn Leu Asp Thr Ile Pro Ala Leu Lys 165 170 175 cct gcg caa ata gac gga gtt gca ata gta tcc gct ata tct atg cag 576 Pro Ala Gln Ile Asp Gly Val Ala Ile Val Ser Ala Ile Ser Met Gln 180 185 190 gaa gat acc gta tct gca aca aga aaa tta aaa aat act ttt ttg aaa 624 Glu Asp Thr Val Ser Ala Thr Arg Lys Leu Lys Asn Thr Phe Leu Lys 195 200 205 caa tat caa act aaa ggc gta ata ttc gat att gac ggt act ctg ctt 672 Gln Tyr Gln Thr Lys Gly Val Ile Phe Asp Ile Asp Gly Thr Leu Leu 210 215 220 gaa act atg aac ata tgg gac aat gta ctt cta aat ctt atg aat acg 720 Glu Thr Met Asn Ile Trp Asp Asn Val Leu Leu Asn Leu Met Asn Thr 225 230 235 240 ctt aat atc cgc tat acc gaa gat gaa ata caa aag ata tgg aat atg 768 Leu Asn Ile Arg Tyr Thr Glu Asp Glu Ile Gln Lys Ile Trp Asn Met 245 250 255 ggt ttt gca gag ctt gca cag ttc agc ata aaa aaa ttc aag ctt gat 816 Gly Phe Ala Glu Leu Ala Gln Phe Ser Ile Lys Lys Phe Lys Leu Asp 260 265 270 atg agt gta aaa gaa ttt tgg caa ctt ata aaa aaa tta tca gtc gaa 864 Met Ser Val Lys Glu Phe Trp Gln Leu Ile Lys Lys Leu Ser Val Glu 275 280 285 gag tat aaa aat agc aaa ata cac tta aaa aaa ggt gca aaa aaa ctg 912 Glu Tyr Lys Asn Ser Lys Ile His Leu Lys Lys Gly Ala Lys Lys Leu 290 295 300 ctt gag tat ctc aaa gaa aaa ggt gta aaa tta gcc ata gca act gcc 960 Leu Glu Tyr Leu Lys Glu Lys Gly Val Lys Leu Ala Ile Ala Thr Ala 305 310 315 320 ctt tgc aaa gaa cag tat gaa ata gtg ctt aca aag aca ggt atc ata 1008 Leu Cys Lys Glu Gln Tyr Glu Ile Val Leu Thr Lys Thr Gly Ile Ile 325 330 335 gac tat ttt gac ata ata gca tca agc gta gat tta aaa atg gaa aaa 1056 Asp Tyr Phe Asp Ile Ile Ala Ser Ser Val Asp Leu Lys Met Glu Lys 340 345 350 tca gat aga caa ata ttt gac tat ata gca aaa aat cta caa gtt cca 1104 Ser Asp Arg Gln Ile Phe Asp Tyr Ile Ala Lys Asn Leu Gln Val Pro 355 360 365 aac aaa aat ttt att ttc ttt gaa gac gac ata aac tcg tca aca ggt 1152 Asn Lys Asn Phe Ile Phe Phe Glu Asp Asp Ile Asn Ser Ser Thr Gly 370 375 380 gca aaa cgt gca gga gta aaa ctg tgc att gta tca aac aag aaa tat 1200 Ala Lys Arg Ala Gly Val Lys Leu Cys Ile Val Ser Asn Lys Lys Tyr 385 390 395 400 aat ggt aac agc aaa ttt gac gct ctc ata gat tat aaa ata gat gat 1248 Asn Gly Asn Ser Lys Phe Asp Ala Leu Ile Asp Tyr Lys Ile Asp Asp 405 410 415 ttt gaa aat aaa ttg ata tat gat gaa ata ata gtg gag aaa aat tag 1296 Phe Glu Asn Lys Leu Ile Tyr Asp Glu Ile Ile Val Glu Lys Asn 420 425 430 <210> 181 <211> 431 <212> PRT <213> Peptostreptococcaceae bacterium <400> 181 Met Lys Asn Ile Asp Tyr Thr Met Tyr Tyr Val Thr Asp Glu Asp Leu 1 5 10 15 Leu Ser Ser Asn His Thr Leu Glu Thr Ser Val Gln Asp Ala Ile Leu 20 25 30 Gly Gly Cys Thr Met Ile Gln Leu Arg Glu Lys His Ser Ser Thr Leu 35 40 45 Asp Phe Tyr Asn Lys Ala Val Lys Ile Lys Ala Ile Cys Asp Lys Tyr 50 55 60 Asn Ile Pro Leu Ile Ile Asn Asp Arg Ile Asp Val Ala Leu Ala Ile 65 70 75 80 Asn Ala Asp Gly Val His Leu Gly Gln Asp Asp Met Pro Leu Asp Ile 85 90 95 Ala Arg Lys Ile Met Gly Asp Gly Lys Ile Ile Gly Ile Ser Thr Ser 100 105 110 Thr Leu Asp Glu Ala Leu Ile Ala Gln Gln Gly Gly Ala Asp Tyr Val 115 120 125 Gly Val Gly Ala Met Tyr Ser Thr Asn Thr Lys Thr Asp Ala Asn Leu 130 135 140 Thr Thr Ile Asp Glu Leu Thr Lys Ile Lys Asn Asn Leu Lys Ile Pro 145 150 155 160 Val Val Ala Ile Gly Gly Ile Asn Leu Asp Thr Ile Pro Ala Leu Lys 165 170 175 Pro Ala Gln Ile Asp Gly Val Ala Ile Val Ser Ala Ile Ser Met Gln 180 185 190 Glu Asp Thr Val Ser Ala Thr Arg Lys Leu Lys Asn Thr Phe Leu Lys 195 200 205 Gln Tyr Gln Thr Lys Gly Val Ile Phe Asp Ile Asp Gly Thr Leu Leu 210 215 220 Glu Thr Met Asn Ile Trp Asp Asn Val Leu Leu Asn Leu Met Asn Thr 225 230 235 240 Leu Asn Ile Arg Tyr Thr Glu Asp Glu Ile Gln Lys Ile Trp Asn Met 245 250 255 Gly Phe Ala Glu Leu Ala Gln Phe Ser Ile Lys Lys Phe Lys Leu Asp 260 265 270 Met Ser Val Lys Glu Phe Trp Gln Leu Ile Lys Lys Leu Ser Val Glu 275 280 285 Glu Tyr Lys Asn Ser Lys Ile His Leu Lys Lys Gly Ala Lys Lys Leu 290 295 300 Leu Glu Tyr Leu Lys Glu Lys Gly Val Lys Leu Ala Ile Ala Thr Ala 305 310 315 320 Leu Cys Lys Glu Gln Tyr Glu Ile Val Leu Thr Lys Thr Gly Ile Ile 325 330 335 Asp Tyr Phe Asp Ile Ile Ala Ser Ser Val Asp Leu Lys Met Glu Lys 340 345 350 Ser Asp Arg Gln Ile Phe Asp Tyr Ile Ala Lys Asn Leu Gln Val Pro 355 360 365 Asn Lys Asn Phe Ile Phe Phe Glu Asp Asp Ile Asn Ser Ser Thr Gly 370 375 380 Ala Lys Arg Ala Gly Val Lys Leu Cys Ile Val Ser Asn Lys Lys Tyr 385 390 395 400 Asn Gly Asn Ser Lys Phe Asp Ala Leu Ile Asp Tyr Lys Ile Asp Asp 405 410 415 Phe Glu Asn Lys Leu Ile Tyr Asp Glu Ile Ile Val Glu Lys Asn 420 425 430 <210> 182 <211> 1365 <212> DNA <213> Atopobium species <220> <221> CDS <222> (1)..(1365) <223> Atopobium sp. ICM42b gene encoding TMP phosphatase [WP_035427744] <400> 182 atg cag gtg acc ggt gca att ttt gat tgc gat gga act ctt gtt gat 48 Met Gln Val Thr Gly Ala Ile Phe Asp Cys Asp Gly Thr Leu Val Asp 1 5 10 15 tca atg cgc gtt tgg cat aac gtt ttt ggc gct gtt ctt cct aaa tat 96 Ser Met Arg Val Trp His Asn Val Phe Gly Ala Val Leu Pro Lys Tyr 20 25 30 ggc aag act att gat tcg gat att ttt gac cgc gta gag gct gtt tcc 144 Gly Lys Thr Ile Asp Ser Asp Ile Phe Asp Arg Val Glu Ala Val Ser 35 40 45 ctc att ggt gga tgt cag att tgc gtt gat gaa ctg gat ttg cct att 192 Leu Ile Gly Gly Cys Gln Ile Cys Val Asp Glu Leu Asp Leu Pro Ile 50 55 60 aca gcg gaa gct ttg tat gaa gag ttc tgc gcg tac gta att gat cag 240 Thr Ala Glu Ala Leu Tyr Glu Glu Phe Cys Ala Tyr Val Ile Asp Gln 65 70 75 80 tac caa cat cat gtt tca atc att ccc ggt gca aag gag ttc tta cag 288 Tyr Gln His His Val Ser Ile Ile Pro Gly Ala Lys Glu Phe Leu Gln 85 90 95 gag ctc tac gat gca ggt att cct atg gcc gtt gct tcg tca act ccc 336 Glu Leu Tyr Asp Ala Gly Ile Pro Met Ala Val Ala Ser Ser Thr Pro 100 105 110 gtg cga gaa gtt cgt gca gct ctg gca gct caa ggt att gag cac ctc 384 Val Arg Glu Val Arg Ala Ala Leu Ala Ala Gln Gly Ile Glu His Leu 115 120 125 ttc aaa aca gtg gtc tca aca gaa gat gtg ggg gga gtg gac aag gtt 432 Phe Lys Thr Val Val Ser Thr Glu Asp Val Gly Gly Val Asp Lys Val 130 135 140 gag cct gat gtt tat ctt gag gct ctt cgc cgt ctt ggc acc gat aag 480 Glu Pro Asp Val Tyr Leu Glu Ala Leu Arg Arg Leu Gly Thr Asp Lys 145 150 155 160 gca act acc tgg gtc ttc gag gat gcc ccg ttt ggc gca cag aca gca 528 Ala Thr Thr Trp Val Phe Glu Asp Ala Pro Phe Gly Ala Gln Thr Ala 165 170 175 caa aat gcg ggc ttt cct gtg gta gcg ctc tac aat gat cat gac ggc 576 Gln Asn Ala Gly Phe Pro Val Val Ala Leu Tyr Asn Asp His Asp Gly 180 185 190 cgc gac ccc gtc ttt atg cgc gag cac tct aac atc ttt gcc cac acc 624 Arg Asp Pro Val Phe Met Arg Glu His Ser Asn Ile Phe Ala His Thr 195 200 205 tac ggc gag ctg tcg ctt ctg cgc ctt cag gac tac gag cgc cct ctg 672 Tyr Gly Glu Leu Ser Leu Leu Arg Leu Gln Asp Tyr Glu Arg Pro Leu 210 215 220 acc gca gcg cct tct ggc gag aaa ccc ctt gag gtc ctt gtt gtg ggc 720 Thr Ala Ala Pro Ser Gly Glu Lys Pro Leu Glu Val Leu Val Val Gly 225 230 235 240 gga tcc cca gag gcg gtt tca cac acg acg ctg tct acc tgc gcc caa 768 Gly Ser Pro Glu Ala Val Ser His Thr Thr Leu Ser Thr Cys Ala Gln 245 250 255 agc gct gac tac ctg ata gcg gtt gac cat ggt gca gat gca tgt cac 816 Ser Ala Asp Tyr Leu Ile Ala Val Asp His Gly Ala Asp Ala Cys His 260 265 270 gct gcc ggc gtg att cca cag ctt gcg ctt gga gac ttt gac tcg gct 864 Ala Ala Gly Val Ile Pro Gln Leu Ala Leu Gly Asp Phe Asp Ser Ala 275 280 285 aca cca gaa act ctg gct tgg ctc aaa gag cag cag gta cct tgc atg 912 Thr Pro Glu Thr Leu Ala Trp Leu Lys Glu Gln Gln Val Pro Cys Met 290 295 300 aag ttt aat gcg gac aag tac gat acc gac ctg gct ctt gct tta aag 960 Lys Phe Asn Ala Asp Lys Tyr Asp Thr Asp Leu Ala Leu Ala Leu Lys 305 310 315 320 tcc gcc gag tac gag gct att cgt aga gat agc aag ctc tct ctt acg 1008 Ser Ala Glu Tyr Glu Ala Ile Arg Arg Asp Ser Lys Leu Ser Leu Thr 325 330 335 gtt gtc tcc aca tct ggc gga cac ctt gat cac cag ctt gta gtg ctt 1056 Val Val Ser Thr Ser Gly Gly His Leu Asp His Gln Leu Val Val Leu 340 345 350 ggt ctt ctc gcc acg tgg gca aag acg ggc aag gca agt gtt cga gtt 1104 Gly Leu Leu Ala Thr Trp Ala Lys Thr Gly Lys Ala Ser Val Arg Val 355 360 365 att gaa aat gac ttt gag atg cgc ttt tta act gca ggc cag gtt gat 1152 Ile Glu Asn Asp Phe Glu Met Arg Phe Leu Thr Ala Gly Gln Val Asp 370 375 380 tct tgg cag ctg agc gca act gat gta ggt aaa aag atg tcc ctt gtg 1200 Ser Trp Gln Leu Ser Ala Thr Asp Val Gly Lys Lys Met Ser Leu Val 385 390 395 400 gct ttg tca gag gag tgc gag gtt tct gag gcc ggc atg aag tgg aat 1248 Ala Leu Ser Glu Glu Cys Glu Val Ser Glu Ala Gly Met Lys Trp Asn 405 410 415 ctt gat cac cag aag ttc acc ttg ctg gga gac gac ggt att tca aat 1296 Leu Asp His Gln Lys Phe Thr Leu Leu Gly Asp Asp Gly Ile Ser Asn 420 425 430 atc gtc gaa tca gac aat tcc tgg gta agg tgc gag aag ggc tgt ctt 1344 Ile Val Glu Ser Asp Asn Ser Trp Val Arg Cys Glu Lys Gly Cys Leu 435 440 445 ttg gtg cag ctt tgg aac taa 1365 Leu Val Gln Leu Trp Asn 450 <210> 183 <211> 454 <212> PRT <213> Atopobium species <400> 183 Met Gln Val Thr Gly Ala Ile Phe Asp Cys Asp Gly Thr Leu Val Asp 1 5 10 15 Ser Met Arg Val Trp His Asn Val Phe Gly Ala Val Leu Pro Lys Tyr 20 25 30 Gly Lys Thr Ile Asp Ser Asp Ile Phe Asp Arg Val Glu Ala Val Ser 35 40 45 Leu Ile Gly Gly Cys Gln Ile Cys Val Asp Glu Leu Asp Leu Pro Ile 50 55 60 Thr Ala Glu Ala Leu Tyr Glu Glu Phe Cys Ala Tyr Val Ile Asp Gln 65 70 75 80 Tyr Gln His His Val Ser Ile Ile Pro Gly Ala Lys Glu Phe Leu Gln 85 90 95 Glu Leu Tyr Asp Ala Gly Ile Pro Met Ala Val Ala Ser Ser Thr Pro 100 105 110 Val Arg Glu Val Arg Ala Ala Leu Ala Ala Gln Gly Ile Glu His Leu 115 120 125 Phe Lys Thr Val Val Ser Thr Glu Asp Val Gly Gly Val Asp Lys Val 130 135 140 Glu Pro Asp Val Tyr Leu Glu Ala Leu Arg Arg Leu Gly Thr Asp Lys 145 150 155 160 Ala Thr Thr Trp Val Phe Glu Asp Ala Pro Phe Gly Ala Gln Thr Ala 165 170 175 Gln Asn Ala Gly Phe Pro Val Val Ala Leu Tyr Asn Asp His Asp Gly 180 185 190 Arg Asp Pro Val Phe Met Arg Glu His Ser Asn Ile Phe Ala His Thr 195 200 205 Tyr Gly Glu Leu Ser Leu Leu Arg Leu Gln Asp Tyr Glu Arg Pro Leu 210 215 220 Thr Ala Ala Pro Ser Gly Glu Lys Pro Leu Glu Val Leu Val Val Gly 225 230 235 240 Gly Ser Pro Glu Ala Val Ser His Thr Thr Leu Ser Thr Cys Ala Gln 245 250 255 Ser Ala Asp Tyr Leu Ile Ala Val Asp His Gly Ala Asp Ala Cys His 260 265 270 Ala Ala Gly Val Ile Pro Gln Leu Ala Leu Gly Asp Phe Asp Ser Ala 275 280 285 Thr Pro Glu Thr Leu Ala Trp Leu Lys Glu Gln Gln Val Pro Cys Met 290 295 300 Lys Phe Asn Ala Asp Lys Tyr Asp Thr Asp Leu Ala Leu Ala Leu Lys 305 310 315 320 Ser Ala Glu Tyr Glu Ala Ile Arg Arg Asp Ser Lys Leu Ser Leu Thr 325 330 335 Val Val Ser Thr Ser Gly Gly His Leu Asp His Gln Leu Val Val Leu 340 345 350 Gly Leu Leu Ala Thr Trp Ala Lys Thr Gly Lys Ala Ser Val Arg Val 355 360 365 Ile Glu Asn Asp Phe Glu Met Arg Phe Leu Thr Ala Gly Gln Val Asp 370 375 380 Ser Trp Gln Leu Ser Ala Thr Asp Val Gly Lys Lys Met Ser Leu Val 385 390 395 400 Ala Leu Ser Glu Glu Cys Glu Val Ser Glu Ala Gly Met Lys Trp Asn 405 410 415 Leu Asp His Gln Lys Phe Thr Leu Leu Gly Asp Asp Gly Ile Ser Asn 420 425 430 Ile Val Glu Ser Asp Asn Ser Trp Val Arg Cys Glu Lys Gly Cys Leu 435 440 445 Leu Val Gln Leu Trp Asn 450 <210> 184 <211> 1365 <212> DNA <213> Atopobium parvulum <220> <221> CDS <222> (1)..(1365) <223> Atopobium parvulum gene encoding TMP phosphatase [WP_035433109] <400> 184 atg cag gtg acc ggt gca att ttt gat tgc gat gga act ctt gtt gat 48 Met Gln Val Thr Gly Ala Ile Phe Asp Cys Asp Gly Thr Leu Val Asp 1 5 10 15 tca atg cac gtt tgg cac aac gtt ttt ggc gct gtt ctt cct aaa tac 96 Ser Met His Val Trp His Asn Val Phe Gly Ala Val Leu Pro Lys Tyr 20 25 30 ggc aag act att gat tcg gat att ttt gac cgc gta gag gct gtt tcc 144 Gly Lys Thr Ile Asp Ser Asp Ile Phe Asp Arg Val Glu Ala Val Ser 35 40 45 ctc att ggt gga tgt cag att tgc gtt gat gag ctg gat ttg cct att 192 Leu Ile Gly Gly Cys Gln Ile Cys Val Asp Glu Leu Asp Leu Pro Ile 50 55 60 aca gcg gaa gct tta tat gaa gag ttc tgc gcg tac gta act gat cag 240 Thr Ala Glu Ala Leu Tyr Glu Glu Phe Cys Ala Tyr Val Thr Asp Gln 65 70 75 80 tac cga cat cat gtt tca atc att ccc ggt gca aag gag ttc tta cag 288 Tyr Arg His His Val Ser Ile Ile Pro Gly Ala Lys Glu Phe Leu Gln 85 90 95 gaa ctc cac gac gca ggc att cct atg gcc gtt gct tcg tca act ccc 336 Glu Leu His Asp Ala Gly Ile Pro Met Ala Val Ala Ser Ser Thr Pro 100 105 110 gtg cga gaa gtt cgt gca gct ctg gca gct caa ggt att gag cac ctc 384 Val Arg Glu Val Arg Ala Ala Leu Ala Ala Gln Gly Ile Glu His Leu 115 120 125 ttt aaa aca gtg gtc tca acg gaa gat gtg ggg gga gtg gac aag gtt 432 Phe Lys Thr Val Val Ser Thr Glu Asp Val Gly Gly Val Asp Lys Val 130 135 140 gag cca gat gtt tac ctt gag gct ctt cgc cgt ctt ggc act gat aag 480 Glu Pro Asp Val Tyr Leu Glu Ala Leu Arg Arg Leu Gly Thr Asp Lys 145 150 155 160 gca act acc tgg gtc ttc gag gat gct ccg ttt ggc gca cag aca gca 528 Ala Thr Thr Trp Val Phe Glu Asp Ala Pro Phe Gly Ala Gln Thr Ala 165 170 175 caa aat gca ggc ttt cct gtg gct gta ctc tac aac gac cac gat ggc 576 Gln Asn Ala Gly Phe Pro Val Ala Val Leu Tyr Asn Asp His Asp Gly 180 185 190 cgc gac ccc gtc ttt atg cgc gag cac tct aac atc ttt gcc cac acc 624 Arg Asp Pro Val Phe Met Arg Glu His Ser Asn Ile Phe Ala His Thr 195 200 205 tac ggc gag ctg tcg ctt ctg cgc ctt cag gac tac gag cgc cct ctg 672 Tyr Gly Glu Leu Ser Leu Leu Arg Leu Gln Asp Tyr Glu Arg Pro Leu 210 215 220 acc gca gcg cct tct ggc gag aaa ccc ctt gag gtc ctt gtt gtg ggc 720 Thr Ala Ala Pro Ser Gly Glu Lys Pro Leu Glu Val Leu Val Val Gly 225 230 235 240 gga tcc cca gag gcg gtt tcg cac acg acg ctg tct acc tgc gcc caa 768 Gly Ser Pro Glu Ala Val Ser His Thr Thr Leu Ser Thr Cys Ala Gln 245 250 255 agc gct gac tac ctg ata gcg gtt gac cat ggc gca gat gtc tgt cac 816 Ser Ala Asp Tyr Leu Ile Ala Val Asp His Gly Ala Asp Val Cys His 260 265 270 gct gcc ggc gtg att cca caa ctt gcg ctt gga gac ttt gac tcc gct 864 Ala Ala Gly Val Ile Pro Gln Leu Ala Leu Gly Asp Phe Asp Ser Ala 275 280 285 aca cca gaa act ctg gct tgg ctc aaa gag cag cag gta cct tgc atg 912 Thr Pro Glu Thr Leu Ala Trp Leu Lys Glu Gln Gln Val Pro Cys Met 290 295 300 aag ttt aat gcg gac aag tac gat acc gac ctg gcg cta gca ttg aaa 960 Lys Phe Asn Ala Asp Lys Tyr Asp Thr Asp Leu Ala Leu Ala Leu Lys 305 310 315 320 tca gct gaa tat gag gca att cgt aga gat agc aag ctc tct ctg acg 1008 Ser Ala Glu Tyr Glu Ala Ile Arg Arg Asp Ser Lys Leu Ser Leu Thr 325 330 335 gtt gtc tcc aca tct ggc ggc cac ctt gat cac cag ctt gta gtg ctt 1056 Val Val Ser Thr Ser Gly Gly His Leu Asp His Gln Leu Val Val Leu 340 345 350 ggt ctt ctc gcc acg tgg gca aag acg ggc aag gca agc gtt cga gtt 1104 Gly Leu Leu Ala Thr Trp Ala Lys Thr Gly Lys Ala Ser Val Arg Val 355 360 365 att gag aat gac ttt gag atg cgc ttt tta gtt gct ggc cag gtg gat 1152 Ile Glu Asn Asp Phe Glu Met Arg Phe Leu Val Ala Gly Gln Val Asp 370 375 380 tct tgg cag ctg aac act atc aat gta ggt aaa aag att tct ctt gta 1200 Ser Trp Gln Leu Asn Thr Ile Asn Val Gly Lys Lys Ile Ser Leu Val 385 390 395 400 gct ttg tca gag gag tgc gag gtt tct gag gcc ggc atg aag tgg aat 1248 Ala Leu Ser Glu Glu Cys Glu Val Ser Glu Ala Gly Met Lys Trp Asn 405 410 415 ctt gat cac cag aag ttc acc ttg ctg gga gac gac ggt att tca aac 1296 Leu Asp His Gln Lys Phe Thr Leu Leu Gly Asp Asp Gly Ile Ser Asn 420 425 430 ata gtt gaa tca gac aat tcc tgg gta agg tgc gag aag ggc tgt ctt 1344 Ile Val Glu Ser Asp Asn Ser Trp Val Arg Cys Glu Lys Gly Cys Leu 435 440 445 ttg gtg cag ctt tgg aac taa 1365 Leu Val Gln Leu Trp Asn 450 <210> 185 <211> 454 <212> PRT <213> Atopobium parvulum <400> 185 Met Gln Val Thr Gly Ala Ile Phe Asp Cys Asp Gly Thr Leu Val Asp 1 5 10 15 Ser Met His Val Trp His Asn Val Phe Gly Ala Val Leu Pro Lys Tyr 20 25 30 Gly Lys Thr Ile Asp Ser Asp Ile Phe Asp Arg Val Glu Ala Val Ser 35 40 45 Leu Ile Gly Gly Cys Gln Ile Cys Val Asp Glu Leu Asp Leu Pro Ile 50 55 60 Thr Ala Glu Ala Leu Tyr Glu Glu Phe Cys Ala Tyr Val Thr Asp Gln 65 70 75 80 Tyr Arg His His Val Ser Ile Ile Pro Gly Ala Lys Glu Phe Leu Gln 85 90 95 Glu Leu His Asp Ala Gly Ile Pro Met Ala Val Ala Ser Ser Thr Pro 100 105 110 Val Arg Glu Val Arg Ala Ala Leu Ala Ala Gln Gly Ile Glu His Leu 115 120 125 Phe Lys Thr Val Val Ser Thr Glu Asp Val Gly Gly Val Asp Lys Val 130 135 140 Glu Pro Asp Val Tyr Leu Glu Ala Leu Arg Arg Leu Gly Thr Asp Lys 145 150 155 160 Ala Thr Thr Trp Val Phe Glu Asp Ala Pro Phe Gly Ala Gln Thr Ala 165 170 175 Gln Asn Ala Gly Phe Pro Val Ala Val Leu Tyr Asn Asp His Asp Gly 180 185 190 Arg Asp Pro Val Phe Met Arg Glu His Ser Asn Ile Phe Ala His Thr 195 200 205 Tyr Gly Glu Leu Ser Leu Leu Arg Leu Gln Asp Tyr Glu Arg Pro Leu 210 215 220 Thr Ala Ala Pro Ser Gly Glu Lys Pro Leu Glu Val Leu Val Val Gly 225 230 235 240 Gly Ser Pro Glu Ala Val Ser His Thr Thr Leu Ser Thr Cys Ala Gln 245 250 255 Ser Ala Asp Tyr Leu Ile Ala Val Asp His Gly Ala Asp Val Cys His 260 265 270 Ala Ala Gly Val Ile Pro Gln Leu Ala Leu Gly Asp Phe Asp Ser Ala 275 280 285 Thr Pro Glu Thr Leu Ala Trp Leu Lys Glu Gln Gln Val Pro Cys Met 290 295 300 Lys Phe Asn Ala Asp Lys Tyr Asp Thr Asp Leu Ala Leu Ala Leu Lys 305 310 315 320 Ser Ala Glu Tyr Glu Ala Ile Arg Arg Asp Ser Lys Leu Ser Leu Thr 325 330 335 Val Val Ser Thr Ser Gly Gly His Leu Asp His Gln Leu Val Val Leu 340 345 350 Gly Leu Leu Ala Thr Trp Ala Lys Thr Gly Lys Ala Ser Val Arg Val 355 360 365 Ile Glu Asn Asp Phe Glu Met Arg Phe Leu Val Ala Gly Gln Val Asp 370 375 380 Ser Trp Gln Leu Asn Thr Ile Asn Val Gly Lys Lys Ile Ser Leu Val 385 390 395 400 Ala Leu Ser Glu Glu Cys Glu Val Ser Glu Ala Gly Met Lys Trp Asn 405 410 415 Leu Asp His Gln Lys Phe Thr Leu Leu Gly Asp Asp Gly Ile Ser Asn 420 425 430 Ile Val Glu Ser Asp Asn Ser Trp Val Arg Cys Glu Lys Gly Cys Leu 435 440 445 Leu Val Gln Leu Trp Asn 450 <210> 186 <211> 1383 <212> DNA <213> Atopobium rimae <220> <221> CDS <222> (1)..(1383) <223> Atopobium rimae gene encoding TMP phosphatase [WP_003148415] <400> 186 atg cag ata acg ggt gca atc ttt gat ctt gat ggg aca ctg gtt gac 48 Met Gln Ile Thr Gly Ala Ile Phe Asp Leu Asp Gly Thr Leu Val Asp 1 5 10 15 tcc atg tgg atg tgg aga aga tcg ttc gga gat gtt tta gaa gac ctg 96 Ser Met Trp Met Trp Arg Arg Ser Phe Gly Asp Val Leu Glu Asp Leu 20 25 30 cat atc aat atg act ccg gat ttt ttt aaa agg gtc gag gcc att tcg 144 His Ile Asn Met Thr Pro Asp Phe Phe Lys Arg Val Glu Ala Ile Ser 35 40 45 ctt tac gat ggt tgc gta gcg tgt att gag gaa ttt aat ctt cct tta 192 Leu Tyr Asp Gly Cys Val Ala Cys Ile Glu Glu Phe Asn Leu Pro Leu 50 55 60 tcc gca gaa gag ctg tat gaa aag ttc ctt ttg tat gta caa acg gta 240 Ser Ala Glu Glu Leu Tyr Glu Lys Phe Leu Leu Tyr Val Gln Thr Val 65 70 75 80 tat tcg cac gat att aaa agc att gcg ggg gct acc gac ttt ctc cag 288 Tyr Ser His Asp Ile Lys Ser Ile Ala Gly Ala Thr Asp Phe Leu Gln 85 90 95 gaa ctt ttt gac gca gga ata cct ctt gcc att gct tct tct acg cca 336 Glu Leu Phe Asp Ala Gly Ile Pro Leu Ala Ile Ala Ser Ser Thr Pro 100 105 110 tct cgt gcc ata cat gtt gct ctt gaa gcc caa ggt atg gag aag ttt 384 Ser Arg Ala Ile His Val Ala Leu Glu Ala Gln Gly Met Glu Lys Phe 115 120 125 ttt aaa gcg gtt gtg tgt acc gaa gac gtc ggg ggt gtc gat aaa gca 432 Phe Lys Ala Val Val Cys Thr Glu Asp Val Gly Gly Val Asp Lys Ala 130 135 140 aaa ccc gat gtc tat ctt gag gct ctc aga cgc ctg ggc acc gat aaa 480 Lys Pro Asp Val Tyr Leu Glu Ala Leu Arg Arg Leu Gly Thr Asp Lys 145 150 155 160 gca cac acg tgg gtc ttt gag gac gct gag ttt ggt gta cat acg gca 528 Ala His Thr Trp Val Phe Glu Asp Ala Glu Phe Gly Val His Thr Ala 165 170 175 caa acc gag ggc ttt ccc gtt gtt gcg ctg ttc aat ggc aaa gac ggc 576 Gln Thr Glu Gly Phe Pro Val Val Ala Leu Phe Asn Gly Lys Asp Gly 180 185 190 cgt gat ctt gag tat atg aag gcg cac tct gat ctt ctc gca cat gat 624 Arg Asp Leu Glu Tyr Met Lys Ala His Ser Asp Leu Leu Ala His Asp 195 200 205 tat cga gaa ctc tct ctt gcc cgc att tac gat tat gaa cgg gtg acg 672 Tyr Arg Glu Leu Ser Leu Ala Arg Ile Tyr Asp Tyr Glu Arg Val Thr 210 215 220 aat cag cca cat ctg ggc gcc tca tcg gct cag aag gtc ttt tcg gtt 720 Asn Gln Pro His Leu Gly Ala Ser Ser Ala Gln Lys Val Phe Ser Val 225 230 235 240 ctc gtt gtt gat gga tct ccc acg cca agt tca gcc gcg ctg gtt tca 768 Leu Val Val Asp Gly Ser Pro Thr Pro Ser Ser Ala Ala Leu Val Ser 245 250 255 gaa ctt tca tca tgc tcg gat tat gtc gtt gct gca gat cgc ggg gca 816 Glu Leu Ser Ser Cys Ser Asp Tyr Val Val Ala Ala Asp Arg Gly Ala 260 265 270 tat atc tgc aag gag gcc ggt gtc gtt cct gat att gcg tgc gga gac 864 Tyr Ile Cys Lys Glu Ala Gly Val Val Pro Asp Ile Ala Cys Gly Asp 275 280 285 ttt gat tcc gtg gga gaa gag aca ctc tct tgg atc cat gca caa aag 912 Phe Asp Ser Val Gly Glu Glu Thr Leu Ser Trp Ile His Ala Gln Lys 290 295 300 gtg cac acg att gct tat cct caa gat aag tac gag acc gat ttg tct 960 Val His Thr Ile Ala Tyr Pro Gln Asp Lys Tyr Glu Thr Asp Leu Ser 305 310 315 320 ctt gca ctc aat gcc gct tgc cat gaa gca acc cgt caa gca ctt ccg 1008 Leu Ala Leu Asn Ala Ala Cys His Glu Ala Thr Arg Gln Ala Leu Pro 325 330 335 ctg tca ctg aca ctt acc tgc gct tcc ggc ggc agg ctt gat cat gag 1056 Leu Ser Leu Thr Leu Thr Cys Ala Ser Gly Gly Arg Leu Asp His Glu 340 345 350 ctt ggt gta gtg ggg ctt ctg gct cga tta agc act gcc tca tgg agg 1104 Leu Gly Val Val Gly Leu Leu Ala Arg Leu Ser Thr Ala Ser Trp Arg 355 360 365 gtg cgg att gtt gag gat gcc ttt gaa gca agg att ctt tcg gca gat 1152 Val Arg Ile Val Glu Asp Ala Phe Glu Ala Arg Ile Leu Ser Ala Asp 370 375 380 acg tat gcg gcg tgg agg ctc tca gaa aaa gat cga gga aag aca ctg 1200 Thr Tyr Ala Ala Trp Arg Leu Ser Glu Lys Asp Arg Gly Lys Thr Leu 385 390 395 400 tcg gtg ctt ccg ctt cag gaa gaa acg gtg att acc gag atc ggt atg 1248 Ser Val Leu Pro Leu Gln Glu Glu Thr Val Ile Thr Glu Ile Gly Met 405 410 415 caa tgg gac ctt gcc tca cga act ttg ctg ctc ctg tct gat gaa gga 1296 Gln Trp Asp Leu Ala Ser Arg Thr Leu Leu Leu Leu Ser Asp Glu Gly 420 425 430 att tcc aat gtg gta caa acg gat gtg gca caa ata cat tgc gag aag 1344 Ile Ser Asn Val Val Gln Thr Asp Val Ala Gln Ile His Cys Glu Lys 435 440 445 ggc aag gcg ctc gtg gtg ctt ctc gca aat gaa tcg tga 1383 Gly Lys Ala Leu Val Val Leu Leu Ala Asn Glu Ser 450 455 460 <210> 187 <211> 460 <212> PRT <213> Atopobium rimae <400> 187 Met Gln Ile Thr Gly Ala Ile Phe Asp Leu Asp Gly Thr Leu Val Asp 1 5 10 15 Ser Met Trp Met Trp Arg Arg Ser Phe Gly Asp Val Leu Glu Asp Leu 20 25 30 His Ile Asn Met Thr Pro Asp Phe Phe Lys Arg Val Glu Ala Ile Ser 35 40 45 Leu Tyr Asp Gly Cys Val Ala Cys Ile Glu Glu Phe Asn Leu Pro Leu 50 55 60 Ser Ala Glu Glu Leu Tyr Glu Lys Phe Leu Leu Tyr Val Gln Thr Val 65 70 75 80 Tyr Ser His Asp Ile Lys Ser Ile Ala Gly Ala Thr Asp Phe Leu Gln 85 90 95 Glu Leu Phe Asp Ala Gly Ile Pro Leu Ala Ile Ala Ser Ser Thr Pro 100 105 110 Ser Arg Ala Ile His Val Ala Leu Glu Ala Gln Gly Met Glu Lys Phe 115 120 125 Phe Lys Ala Val Val Cys Thr Glu Asp Val Gly Gly Val Asp Lys Ala 130 135 140 Lys Pro Asp Val Tyr Leu Glu Ala Leu Arg Arg Leu Gly Thr Asp Lys 145 150 155 160 Ala His Thr Trp Val Phe Glu Asp Ala Glu Phe Gly Val His Thr Ala 165 170 175 Gln Thr Glu Gly Phe Pro Val Val Ala Leu Phe Asn Gly Lys Asp Gly 180 185 190 Arg Asp Leu Glu Tyr Met Lys Ala His Ser Asp Leu Leu Ala His Asp 195 200 205 Tyr Arg Glu Leu Ser Leu Ala Arg Ile Tyr Asp Tyr Glu Arg Val Thr 210 215 220 Asn Gln Pro His Leu Gly Ala Ser Ser Ala Gln Lys Val Phe Ser Val 225 230 235 240 Leu Val Val Asp Gly Ser Pro Thr Pro Ser Ser Ala Ala Leu Val Ser 245 250 255 Glu Leu Ser Ser Cys Ser Asp Tyr Val Val Ala Ala Asp Arg Gly Ala 260 265 270 Tyr Ile Cys Lys Glu Ala Gly Val Val Pro Asp Ile Ala Cys Gly Asp 275 280 285 Phe Asp Ser Val Gly Glu Glu Thr Leu Ser Trp Ile His Ala Gln Lys 290 295 300 Val His Thr Ile Ala Tyr Pro Gln Asp Lys Tyr Glu Thr Asp Leu Ser 305 310 315 320 Leu Ala Leu Asn Ala Ala Cys His Glu Ala Thr Arg Gln Ala Leu Pro 325 330 335 Leu Ser Leu Thr Leu Thr Cys Ala Ser Gly Gly Arg Leu Asp His Glu 340 345 350 Leu Gly Val Val Gly Leu Leu Ala Arg Leu Ser Thr Ala Ser Trp Arg 355 360 365 Val Arg Ile Val Glu Asp Ala Phe Glu Ala Arg Ile Leu Ser Ala Asp 370 375 380 Thr Tyr Ala Ala Trp Arg Leu Ser Glu Lys Asp Arg Gly Lys Thr Leu 385 390 395 400 Ser Val Leu Pro Leu Gln Glu Glu Thr Val Ile Thr Glu Ile Gly Met 405 410 415 Gln Trp Asp Leu Ala Ser Arg Thr Leu Leu Leu Leu Ser Asp Glu Gly 420 425 430 Ile Ser Asn Val Val Gln Thr Asp Val Ala Gln Ile His Cys Glu Lys 435 440 445 Gly Lys Ala Leu Val Val Leu Leu Ala Asn Glu Ser 450 455 460 <210> 188 <211> 1380 <212> DNA <213> Olsenella uli <220> <221> CDS <222> (1)..(1380) <223> Olsenella uli gene encoding TMP phosphatase [WP_013251930] <400> 188 atg ccc atc aag gcc gcc atc ttc gac tgt gac gga acg ctg gtc gac 48 Met Pro Ile Lys Ala Ala Ile Phe Asp Cys Asp Gly Thr Leu Val Asp 1 5 10 15 tcc atg ccc ctg tgg cat gac gtg acg gtc gaa ctg ctg cgc cgc cac 96 Ser Met Pro Leu Trp His Asp Val Thr Val Glu Leu Leu Arg Arg His 20 25 30 cat gtc gcc gac gcc gag gag gcg ttc gtc cgc acc gag tcg ctt ccc 144 His Val Ala Asp Ala Glu Glu Ala Phe Val Arg Thr Glu Ser Leu Pro 35 40 45 atg gtc gag atg tgc cat gcc ttc cac gac gag tgg ggc gtt gag gcc 192 Met Val Glu Met Cys His Ala Phe His Asp Glu Trp Gly Val Glu Ala 50 55 60 gag ggc gag gag ctg gtg cgc gag ctg gtc gat atg gtc cgc gag ggg 240 Glu Gly Glu Glu Leu Val Arg Glu Leu Val Asp Met Val Arg Glu Gly 65 70 75 80 tat cgc agc cgg gtt agc ctg ctg ccg ggc tgc cgg gcg ttt ctg gac 288 Tyr Arg Ser Arg Val Ser Leu Leu Pro Gly Cys Arg Ala Phe Leu Asp 85 90 95 gag ctg gcg tct gcg ggc gtc cgc atg gtc gtc gcg tcg tcg acg gct 336 Glu Leu Ala Ser Ala Gly Val Arg Met Val Val Ala Ser Ser Thr Ala 100 105 110 ccg gag gag ctc tcc gtc gcg cta tcg gcg cag ggg gtc gac ggc tac 384 Pro Glu Glu Leu Ser Val Ala Leu Ser Ala Gln Gly Val Asp Gly Tyr 115 120 125 ttc gag cgg gtc ttc tcc acg gga ggc ccc ata cgc agc aag gac tac 432 Phe Glu Arg Val Phe Ser Thr Gly Gly Pro Ile Arg Ser Lys Asp Tyr 130 135 140 ccg gac atc tgg gag ctg gtc ctg gac tac ctg ggc acc gac ccg gct 480 Pro Asp Ile Trp Glu Leu Val Leu Asp Tyr Leu Gly Thr Asp Pro Ala 145 150 155 160 gac acc tgg gtc ttc gag gac gcc ccg ttt ggg atg cgg acg gcc cga 528 Asp Thr Trp Val Phe Glu Asp Ala Pro Phe Gly Met Arg Thr Ala Arg 165 170 175 tcg gtc ggc gcc aac acc gtc tgc ctg ttc agc cca cac ggg gac cgc 576 Ser Val Gly Ala Asn Thr Val Cys Leu Phe Ser Pro His Gly Asp Arg 180 185 190 gac ctt gcg gcc tgc gag cgc tac gct gac ata ctg gtc cac agc tac 624 Asp Leu Ala Ala Cys Glu Arg Tyr Ala Asp Ile Leu Val His Ser Tyr 195 200 205 cac gag cta tcg ctc gcc ctg ctg gac gac tac gcc cgt ccg ccg caa 672 His Glu Leu Ser Leu Ala Leu Leu Asp Asp Tyr Ala Arg Pro Pro Gln 210 215 220 gcg tcc ccc tcg gcc cac cct cgc ctc gcg ccg ctt cgc gtc ctc gtc 720 Ala Ser Pro Ser Ala His Pro Arg Leu Ala Pro Leu Arg Val Leu Val 225 230 235 240 gtg ggc gcc tcg ccc gag cgc ccg tct tcg gcg ctg ctc cgc tcc ctg 768 Val Gly Ala Ser Pro Glu Arg Pro Ser Ser Ala Leu Leu Arg Ser Leu 245 250 255 gcc gcc agt acc gac tac gtc atc gcc gcc gac gcc ggg gcc gac gcg 816 Ala Ala Ser Thr Asp Tyr Val Ile Ala Ala Asp Ala Gly Ala Asp Ala 260 265 270 ctg cgc tcc tgt ggc atc gcc ccc gac gtc ttc tgc ggc gac gcc gac 864 Leu Arg Ser Cys Gly Ile Ala Pro Asp Val Phe Cys Gly Asp Ala Asp 275 280 285 tcg gca acg ggc gaa tcg gct gcg tgg gcc cgc tcg gtc gcc cgt gcg 912 Ser Ala Thr Gly Glu Ser Ala Ala Trp Ala Arg Ser Val Ala Arg Ala 290 295 300 gac ata gag ttt ccc tcc gag aag tac gcg acc gac ctc gcc ctc gcc 960 Asp Ile Glu Phe Pro Ser Glu Lys Tyr Ala Thr Asp Leu Ala Leu Ala 305 310 315 320 atc tcc tgc gcc cgc cat gag gcc gct cga cgc aac gcg cgg ctg gag 1008 Ile Ser Cys Ala Arg His Glu Ala Ala Arg Arg Asn Ala Arg Leu Glu 325 330 335 ctc acg ctg acc ggc gtc acg ggc ggc agg ccc gac cac gcc ctt gcc 1056 Leu Thr Leu Thr Gly Val Thr Gly Gly Arg Pro Asp His Ala Leu Ala 340 345 350 gtc gtg ggt cag ctc gcg cgg aac gct gac gcc tcg ccg cgc atc gtg 1104 Val Val Gly Gln Leu Ala Arg Asn Ala Asp Ala Ser Pro Arg Ile Val 355 360 365 gag gac ggc ttc gag tgc cga ctg ctc agc ccc tct ggc act gcg tgc 1152 Glu Asp Gly Phe Glu Cys Arg Leu Leu Ser Pro Ser Gly Thr Ala Cys 370 375 380 tgg gag ctg ggt ggg gcc cac gtg cca gcc gcc ggg gtc gag ggg acg 1200 Trp Glu Leu Gly Gly Ala His Val Pro Ala Ala Gly Val Glu Gly Thr 385 390 395 400 ctc ttc tcg gcc att ccc gtg gca gag ggg acc atg ctc tcc gag cgg 1248 Leu Phe Ser Ala Ile Pro Val Ala Glu Gly Thr Met Leu Ser Glu Arg 405 410 415 ggc ttc aag tgg gag ctg gat cat cgt gag ctg ccc ctt ctg ggg gat 1296 Gly Phe Lys Trp Glu Leu Asp His Arg Glu Leu Pro Leu Leu Gly Asp 420 425 430 gag gga atc tcg aac gtg gtc acg tcc gcg acg gcc agc gtc gag tgc 1344 Glu Gly Ile Ser Asn Val Val Thr Ser Ala Thr Ala Ser Val Glu Cys 435 440 445 cat gcc ggc gca gtt gcg gcg ttc ctg ttg gca tag 1380 His Ala Gly Ala Val Ala Ala Phe Leu Leu Ala 450 455 <210> 189 <211> 459 <212> PRT <213> Olsenella uli <400> 189 Met Pro Ile Lys Ala Ala Ile Phe Asp Cys Asp Gly Thr Leu Val Asp 1 5 10 15 Ser Met Pro Leu Trp His Asp Val Thr Val Glu Leu Leu Arg Arg His 20 25 30 His Val Ala Asp Ala Glu Glu Ala Phe Val Arg Thr Glu Ser Leu Pro 35 40 45 Met Val Glu Met Cys His Ala Phe His Asp Glu Trp Gly Val Glu Ala 50 55 60 Glu Gly Glu Glu Leu Val Arg Glu Leu Val Asp Met Val Arg Glu Gly 65 70 75 80 Tyr Arg Ser Arg Val Ser Leu Leu Pro Gly Cys Arg Ala Phe Leu Asp 85 90 95 Glu Leu Ala Ser Ala Gly Val Arg Met Val Val Ala Ser Ser Thr Ala 100 105 110 Pro Glu Glu Leu Ser Val Ala Leu Ser Ala Gln Gly Val Asp Gly Tyr 115 120 125 Phe Glu Arg Val Phe Ser Thr Gly Gly Pro Ile Arg Ser Lys Asp Tyr 130 135 140 Pro Asp Ile Trp Glu Leu Val Leu Asp Tyr Leu Gly Thr Asp Pro Ala 145 150 155 160 Asp Thr Trp Val Phe Glu Asp Ala Pro Phe Gly Met Arg Thr Ala Arg 165 170 175 Ser Val Gly Ala Asn Thr Val Cys Leu Phe Ser Pro His Gly Asp Arg 180 185 190 Asp Leu Ala Ala Cys Glu Arg Tyr Ala Asp Ile Leu Val His Ser Tyr 195 200 205 His Glu Leu Ser Leu Ala Leu Leu Asp Asp Tyr Ala Arg Pro Pro Gln 210 215 220 Ala Ser Pro Ser Ala His Pro Arg Leu Ala Pro Leu Arg Val Leu Val 225 230 235 240 Val Gly Ala Ser Pro Glu Arg Pro Ser Ser Ala Leu Leu Arg Ser Leu 245 250 255 Ala Ala Ser Thr Asp Tyr Val Ile Ala Ala Asp Ala Gly Ala Asp Ala 260 265 270 Leu Arg Ser Cys Gly Ile Ala Pro Asp Val Phe Cys Gly Asp Ala Asp 275 280 285 Ser Ala Thr Gly Glu Ser Ala Ala Trp Ala Arg Ser Val Ala Arg Ala 290 295 300 Asp Ile Glu Phe Pro Ser Glu Lys Tyr Ala Thr Asp Leu Ala Leu Ala 305 310 315 320 Ile Ser Cys Ala Arg His Glu Ala Ala Arg Arg Asn Ala Arg Leu Glu 325 330 335 Leu Thr Leu Thr Gly Val Thr Gly Gly Arg Pro Asp His Ala Leu Ala 340 345 350 Val Val Gly Gln Leu Ala Arg Asn Ala Asp Ala Ser Pro Arg Ile Val 355 360 365 Glu Asp Gly Phe Glu Cys Arg Leu Leu Ser Pro Ser Gly Thr Ala Cys 370 375 380 Trp Glu Leu Gly Gly Ala His Val Pro Ala Ala Gly Val Glu Gly Thr 385 390 395 400 Leu Phe Ser Ala Ile Pro Val Ala Glu Gly Thr Met Leu Ser Glu Arg 405 410 415 Gly Phe Lys Trp Glu Leu Asp His Arg Glu Leu Pro Leu Leu Gly Asp 420 425 430 Glu Gly Ile Ser Asn Val Val Thr Ser Ala Thr Ala Ser Val Glu Cys 435 440 445 His Ala Gly Ala Val Ala Ala Phe Leu Leu Ala 450 455 <210> 190 <211> 762 <212> DNA <213> Atopobium minutum <220> <221> CDS <222> (1)..(762) <223> Atopobium minutum gene encoding TMP phosphatase [KRN55115] <400> 190 atg tgg gct aaa acc tct cga cat tgt acg caa aaa ggc ttt acc atg 48 Met Trp Ala Lys Thr Ser Arg His Cys Thr Gln Lys Gly Phe Thr Met 1 5 10 15 aac cct gca cgc att tta ttt gat gga gga act tgt atg gca ata agc 96 Asn Pro Ala Arg Ile Leu Phe Asp Gly Gly Thr Cys Met Ala Ile Ser 20 25 30 ggc gca atc ttt gac tgt gac ggc acg ctg gtt gat tct atg tat atg 144 Gly Ala Ile Phe Asp Cys Asp Gly Thr Leu Val Asp Ser Met Tyr Met 35 40 45 tgg tgg gac gcc ttt ccc cgc ctg ctt gcc agc cat ggc ttt gct atg 192 Trp Trp Asp Ala Phe Pro Arg Leu Leu Ala Ser His Gly Phe Ala Met 50 55 60 acg cct cag atc gag aaa atc ttg cat gag tgt gag gcg gtc agc ttg 240 Thr Pro Gln Ile Glu Lys Ile Leu His Glu Cys Glu Ala Val Ser Leu 65 70 75 80 gat gaa gag atc cat acg ctg cgc aac gct ctt gct att ccc gct tct 288 Asp Glu Glu Ile His Thr Leu Arg Asn Ala Leu Ala Ile Pro Ala Ser 85 90 95 gcc gag cag cta gca caa gaa tta tcc cag aat att agc aat gcg tat 336 Ala Glu Gln Leu Ala Gln Glu Leu Ser Gln Asn Ile Ser Asn Ala Tyr 100 105 110 gcc tca gag atc aaa gca tgg cct gcc gtt aag ccg ttc ttg gat cag 384 Ala Ser Glu Ile Lys Ala Trp Pro Ala Val Lys Pro Phe Leu Asp Gln 115 120 125 ctc aaa gac gca ggt atc ccc atg atc att tgt act tct acc gga gcc 432 Leu Lys Asp Ala Gly Ile Pro Met Ile Ile Cys Thr Ser Thr Gly Ala 130 135 140 aaa gaa gtt ggt ctg tgc atg gat cat ctt ggt ttg tcc aag ttt ttt 480 Lys Glu Val Gly Leu Cys Met Asp His Leu Gly Leu Ser Lys Phe Phe 145 150 155 160 gta gat att gtc agc gcg gaa gaa aac aat ttc acc aaa act gag cca 528 Val Asp Ile Val Ser Ala Glu Glu Asn Asn Phe Thr Lys Thr Glu Pro 165 170 175 gat atc tat tac tat gcg cta aaa aag ctt ggt acc act aaa gag aca 576 Asp Ile Tyr Tyr Tyr Ala Leu Lys Lys Leu Gly Thr Thr Lys Glu Thr 180 185 190 acc tgg gta ttt gag gat gct ccg ttt ggc ctt act acc tct gag cgt 624 Thr Trp Val Phe Glu Asp Ala Pro Phe Gly Leu Thr Thr Ser Glu Arg 195 200 205 gca gga ttt cct aat gtg tgc gtc ttt aat gcg cac gat aag cgc gat 672 Ala Gly Phe Pro Asn Val Cys Val Phe Asn Ala His Asp Lys Arg Asp 210 215 220 gag gac ttt ttg cgt ctt cat gct acg ttg ttt acg cac ata tat gag 720 Glu Asp Phe Leu Arg Leu His Ala Thr Leu Phe Thr His Ile Tyr Glu 225 230 235 240 gat att tcc ctt gcg gat ttg cag tcg tac ccc acc aag taa 762 Asp Ile Ser Leu Ala Asp Leu Gln Ser Tyr Pro Thr Lys 245 250 <210> 191 <211> 253 <212> PRT <213> Atopobium minutum <400> 191 Met Trp Ala Lys Thr Ser Arg His Cys Thr Gln Lys Gly Phe Thr Met 1 5 10 15 Asn Pro Ala Arg Ile Leu Phe Asp Gly Gly Thr Cys Met Ala Ile Ser 20 25 30 Gly Ala Ile Phe Asp Cys Asp Gly Thr Leu Val Asp Ser Met Tyr Met 35 40 45 Trp Trp Asp Ala Phe Pro Arg Leu Leu Ala Ser His Gly Phe Ala Met 50 55 60 Thr Pro Gln Ile Glu Lys Ile Leu His Glu Cys Glu Ala Val Ser Leu 65 70 75 80 Asp Glu Glu Ile His Thr Leu Arg Asn Ala Leu Ala Ile Pro Ala Ser 85 90 95 Ala Glu Gln Leu Ala Gln Glu Leu Ser Gln Asn Ile Ser Asn Ala Tyr 100 105 110 Ala Ser Glu Ile Lys Ala Trp Pro Ala Val Lys Pro Phe Leu Asp Gln 115 120 125 Leu Lys Asp Ala Gly Ile Pro Met Ile Ile Cys Thr Ser Thr Gly Ala 130 135 140 Lys Glu Val Gly Leu Cys Met Asp His Leu Gly Leu Ser Lys Phe Phe 145 150 155 160 Val Asp Ile Val Ser Ala Glu Glu Asn Asn Phe Thr Lys Thr Glu Pro 165 170 175 Asp Ile Tyr Tyr Tyr Ala Leu Lys Lys Leu Gly Thr Thr Lys Glu Thr 180 185 190 Thr Trp Val Phe Glu Asp Ala Pro Phe Gly Leu Thr Thr Ser Glu Arg 195 200 205 Ala Gly Phe Pro Asn Val Cys Val Phe Asn Ala His Asp Lys Arg Asp 210 215 220 Glu Asp Phe Leu Arg Leu His Ala Thr Leu Phe Thr His Ile Tyr Glu 225 230 235 240 Asp Ile Ser Leu Ala Asp Leu Gln Ser Tyr Pro Thr Lys 245 250 <210> 192 <211> 648 <212> DNA <213> Syntrophomonas wolfei <220> <221> CDS <222> (1)..(648) <223> Syntrophomonas wolfei gene encoding TMP phosphatase [WP_011640074] <400> 192 atg gga gag aaa tta ata att ttt atg gat ttc gat ggc act att tct 48 Met Gly Glu Lys Leu Ile Ile Phe Met Asp Phe Asp Gly Thr Ile Ser 1 5 10 15 cgg gag gat gtc tgc aat aag atg gca gcc agg tat gcc ggc agg gac 96 Arg Glu Asp Val Cys Asn Lys Met Ala Ala Arg Tyr Ala Gly Arg Asp 20 25 30 tgg gag gaa ata aac cgc ctc tgg gaa gag gga ggt att act act gga 144 Trp Glu Glu Ile Asn Arg Leu Trp Glu Glu Gly Gly Ile Thr Thr Gly 35 40 45 gag tgc gcc agt cgt att ctt tca tca atg gag gta ggg gcg gct gaa 192 Glu Cys Ala Ser Arg Ile Leu Ser Ser Met Glu Val Gly Ala Ala Glu 50 55 60 ttg gag gcc ttt ttt cag gct cag gaa gta gac ccc ggc ttt tcc cct 240 Leu Glu Ala Phe Phe Gln Ala Gln Glu Val Asp Pro Gly Phe Ser Pro 65 70 75 80 ttc ctg gac tgg gta caa aaa aat cag cac ctc ccc att ata ttg agc 288 Phe Leu Asp Trp Val Gln Lys Asn Gln His Leu Pro Ile Ile Leu Ser 85 90 95 gat ggt tat gac cgc tat ata aaa agc ata tta cgg ggc cag ggc tgg 336 Asp Gly Tyr Asp Arg Tyr Ile Lys Ser Ile Leu Arg Gly Gln Gly Trp 100 105 110 gaa atc gag ttt tat gcc aat aaa tta tac tgg gat gac gcc tgg cgg 384 Glu Ile Glu Phe Tyr Ala Asn Lys Leu Tyr Trp Asp Asp Ala Trp Arg 115 120 125 atg gaa tcg ccc tac ctg gat gaa gaa tgc ttt aaa tgt ggg gta tgc 432 Met Glu Ser Pro Tyr Leu Asp Glu Glu Cys Phe Lys Cys Gly Val Cys 130 135 140 aag agc aag ata atc cag gaa aga agt tta ccc ggc tat ctc aca gta 480 Lys Ser Lys Ile Ile Gln Glu Arg Ser Leu Pro Gly Tyr Leu Thr Val 145 150 155 160 tat atc gga gat ggc tac tcc gat ttc tgc ccg gcg gcc tct tgt gat 528 Tyr Ile Gly Asp Gly Tyr Ser Asp Phe Cys Pro Ala Ala Ser Cys Asp 165 170 175 att gtt ttt gcc aaa aat gaa ctg gcc ggc tac tgc cag aaa gag ggt 576 Ile Val Phe Ala Lys Asn Glu Leu Ala Gly Tyr Cys Gln Lys Glu Gly 180 185 190 tta act tac tac ccc tac cgg gat ttt cac gat att ctc cag caa ctg 624 Leu Thr Tyr Tyr Pro Tyr Arg Asp Phe His Asp Ile Leu Gln Gln Leu 195 200 205 ccg agg att gtt agc agg atg tag 648 Pro Arg Ile Val Ser Arg Met 210 215 <210> 193 <211> 215 <212> PRT <213> Syntrophomonas wolfei <400> 193 Met Gly Glu Lys Leu Ile Ile Phe Met Asp Phe Asp Gly Thr Ile Ser 1 5 10 15 Arg Glu Asp Val Cys Asn Lys Met Ala Ala Arg Tyr Ala Gly Arg Asp 20 25 30 Trp Glu Glu Ile Asn Arg Leu Trp Glu Glu Gly Gly Ile Thr Thr Gly 35 40 45 Glu Cys Ala Ser Arg Ile Leu Ser Ser Met Glu Val Gly Ala Ala Glu 50 55 60 Leu Glu Ala Phe Phe Gln Ala Gln Glu Val Asp Pro Gly Phe Ser Pro 65 70 75 80 Phe Leu Asp Trp Val Gln Lys Asn Gln His Leu Pro Ile Ile Leu Ser 85 90 95 Asp Gly Tyr Asp Arg Tyr Ile Lys Ser Ile Leu Arg Gly Gln Gly Trp 100 105 110 Glu Ile Glu Phe Tyr Ala Asn Lys Leu Tyr Trp Asp Asp Ala Trp Arg 115 120 125 Met Glu Ser Pro Tyr Leu Asp Glu Glu Cys Phe Lys Cys Gly Val Cys 130 135 140 Lys Ser Lys Ile Ile Gln Glu Arg Ser Leu Pro Gly Tyr Leu Thr Val 145 150 155 160 Tyr Ile Gly Asp Gly Tyr Ser Asp Phe Cys Pro Ala Ala Ser Cys Asp 165 170 175 Ile Val Phe Ala Lys Asn Glu Leu Ala Gly Tyr Cys Gln Lys Glu Gly 180 185 190 Leu Thr Tyr Tyr Pro Tyr Arg Asp Phe His Asp Ile Leu Gln Gln Leu 195 200 205 Pro Arg Ile Val Ser Arg Met 210 215 <210> 194 <211> 666 <212> DNA <213> Desulfitobacterium hafniense <220> <221> CDS <222> (1)..(666) <223> Desulfitobacterium hafniense gene encoding TMP phosphatase [WP_018212876] <400> 194 atg gag gaa ttg aac agt att ttc ttc gtg gat ttt gac ggc acc atc 48 Met Glu Glu Leu Asn Ser Ile Phe Phe Val Asp Phe Asp Gly Thr Ile 1 5 10 15 gtc act cag gat atg tgt gca gtc ctc gtt gaa acc ttg gcc ggg gaa 96 Val Thr Gln Asp Met Cys Ala Val Leu Val Glu Thr Leu Ala Gly Glu 20 25 30 gga tgg cgg gag att aat gaa ctt tgg gaa aga aaa gag ctt tcc acc 144 Gly Trp Arg Glu Ile Asn Glu Leu Trp Glu Arg Lys Glu Leu Ser Thr 35 40 45 ctg gag tgc gcc cgc cgg acc ttt aaa ctc ttt aac agc aat gac ccg 192 Leu Glu Cys Ala Arg Arg Thr Phe Lys Leu Phe Asn Ser Asn Asp Pro 50 55 60 gaa gtt ttt cgc cag ctt atc ggg cag gcg gtg ttc gat ccc gga ttt 240 Glu Val Phe Arg Gln Leu Ile Gly Gln Ala Val Phe Asp Pro Gly Phe 65 70 75 80 tta gat ttt gcc gct ttt tgt gaa cag aga gga ttt ccc ctc atc att 288 Leu Asp Phe Ala Ala Phe Cys Glu Gln Arg Gly Phe Pro Leu Ile Ile 85 90 95 ctc agc gac gga tat gat ttc tat att gag tac ctc ttg caa aga gag 336 Leu Ser Asp Gly Tyr Asp Phe Tyr Ile Glu Tyr Leu Leu Gln Arg Glu 100 105 110 gga ttg aac ctg cca tac tat gcc aac aaa ttg ctg ttt gct ccc caa 384 Gly Leu Asn Leu Pro Tyr Tyr Ala Asn Lys Leu Leu Phe Ala Pro Gln 115 120 125 ctt gac gta gaa acc ccc tac agc tcc ggc gaa tgt gat cta tgc ggg 432 Leu Asp Val Glu Thr Pro Tyr Ser Ser Gly Glu Cys Asp Leu Cys Gly 130 135 140 gtc tgc aaa ctg cag ctg atg gaa aaa ttg ctt aaa ccc ggt tgc cga 480 Val Cys Lys Leu Gln Leu Met Glu Lys Leu Leu Lys Pro Gly Cys Arg 145 150 155 160 tcc gtc tat atc gga gat ggg act tcc gat ttt tgc ccg gcg gaa agg 528 Ser Val Tyr Ile Gly Asp Gly Thr Ser Asp Phe Cys Pro Ala Glu Arg 165 170 175 gcg gat aag gtc ttt gcc agg agc agg ctt tat cag cat tgc cag gag 576 Ala Asp Lys Val Phe Ala Arg Ser Arg Leu Tyr Gln His Cys Gln Glu 180 185 190 gtg ggc aaa gaa gcc cag cta ttc caa tcg ttt cag gat att ctt cag 624 Val Gly Lys Glu Ala Gln Leu Phe Gln Ser Phe Gln Asp Ile Leu Gln 195 200 205 aca gtt gaa cat tgg gga agg gaa gag gag gaa ggg act tga 666 Thr Val Glu His Trp Gly Arg Glu Glu Glu Glu Gly Thr 210 215 220 <210> 195 <211> 221 <212> PRT <213> Desulfitobacterium hafniense <400> 195 Met Glu Glu Leu Asn Ser Ile Phe Phe Val Asp Phe Asp Gly Thr Ile 1 5 10 15 Val Thr Gln Asp Met Cys Ala Val Leu Val Glu Thr Leu Ala Gly Glu 20 25 30 Gly Trp Arg Glu Ile Asn Glu Leu Trp Glu Arg Lys Glu Leu Ser Thr 35 40 45 Leu Glu Cys Ala Arg Arg Thr Phe Lys Leu Phe Asn Ser Asn Asp Pro 50 55 60 Glu Val Phe Arg Gln Leu Ile Gly Gln Ala Val Phe Asp Pro Gly Phe 65 70 75 80 Leu Asp Phe Ala Ala Phe Cys Glu Gln Arg Gly Phe Pro Leu Ile Ile 85 90 95 Leu Ser Asp Gly Tyr Asp Phe Tyr Ile Glu Tyr Leu Leu Gln Arg Glu 100 105 110 Gly Leu Asn Leu Pro Tyr Tyr Ala Asn Lys Leu Leu Phe Ala Pro Gln 115 120 125 Leu Asp Val Glu Thr Pro Tyr Ser Ser Gly Glu Cys Asp Leu Cys Gly 130 135 140 Val Cys Lys Leu Gln Leu Met Glu Lys Leu Leu Lys Pro Gly Cys Arg 145 150 155 160 Ser Val Tyr Ile Gly Asp Gly Thr Ser Asp Phe Cys Pro Ala Glu Arg 165 170 175 Ala Asp Lys Val Phe Ala Arg Ser Arg Leu Tyr Gln His Cys Gln Glu 180 185 190 Val Gly Lys Glu Ala Gln Leu Phe Gln Ser Phe Gln Asp Ile Leu Gln 195 200 205 Thr Val Glu His Trp Gly Arg Glu Glu Glu Glu Gly Thr 210 215 220 <210> 196 <211> 642 <212> DNA <213> Pelotomaculum thermopropionicum <220> <221> CDS <222> (1)..(642) <223> Pelotomaculum thermopropionicum gene encoding TMP phosphatase [WP_012032097] <400> 196 atg gaa aaa gtt ttt ttt gtt gat ttt gac ggg acg gta acc aaa aag 48 Met Glu Lys Val Phe Phe Val Asp Phe Asp Gly Thr Val Thr Lys Lys 1 5 10 15 gat acc tgc gtg gcc atg atc gag gcc ttt gcc ggc ggc aac tgg aga 96 Asp Thr Cys Val Ala Met Ile Glu Ala Phe Ala Gly Gly Asn Trp Arg 20 25 30 gag att aac gag gcg tgg gaa aga aaa gaa att tcc acg gaa gaa tgt 144 Glu Ile Asn Glu Ala Trp Glu Arg Lys Glu Ile Ser Thr Glu Glu Cys 35 40 45 gca aac atg atc ttc agg ctt ttc cgc gcc ggc att gaa gac atc agg 192 Ala Asn Met Ile Phe Arg Leu Phe Arg Ala Gly Ile Glu Asp Ile Arg 50 55 60 aag ctt ttg gac ggt atc gag ata gac ggc cat ttt aaa gat ttt ctt 240 Lys Leu Leu Asp Gly Ile Glu Ile Asp Gly His Phe Lys Asp Phe Leu 65 70 75 80 tct ttt tgc cgg gaa aga ggc tat aaa ata tac atc ctc agc gac ggt 288 Ser Phe Cys Arg Glu Arg Gly Tyr Lys Ile Tyr Ile Leu Ser Asp Gly 85 90 95 tac gac ttt tgc att gag acg gtg ttt aaa aaa cac gga ata gag ctg 336 Tyr Asp Phe Cys Ile Glu Thr Val Phe Lys Lys His Gly Ile Glu Leu 100 105 110 ccg tac tat gcc aac aaa atg gtt tac ggc aat ggt ttt aaa ata gaa 384 Pro Tyr Tyr Ala Asn Lys Met Val Tyr Gly Asn Gly Phe Lys Ile Glu 115 120 125 tgc ttc agg ccc aac ccg gcc tgc ggt att tgc ggg acc tgc aag acc 432 Cys Phe Arg Pro Asn Pro Ala Cys Gly Ile Cys Gly Thr Cys Lys Thr 130 135 140 aag ctg att gag gag ctt aaa ggg gac ggc agc cag gtt att tac att 480 Lys Leu Ile Glu Glu Leu Lys Gly Asp Gly Ser Gln Val Ile Tyr Ile 145 150 155 160 ggc gac gga tat tcg gac aca tgc ccg gcc atg aaa gcc gat gtg gtt 528 Gly Asp Gly Tyr Ser Asp Thr Cys Pro Ala Met Lys Ala Asp Val Val 165 170 175 ttt gcc aag gga gta ttg tac agg cat tgc cgg gaa aac ggc aaa aag 576 Phe Ala Lys Gly Val Leu Tyr Arg His Cys Arg Glu Asn Gly Lys Lys 180 185 190 gct att tat tat aat aac ttt ggt gat att att aat tat ttt ttc caa 624 Ala Ile Tyr Tyr Asn Asn Phe Gly Asp Ile Ile Asn Tyr Phe Phe Gln 195 200 205 ata aaa aaa agt ttg taa 642 Ile Lys Lys Ser Leu 210 <210> 197 <211> 213 <212> PRT <213> Pelotomaculum thermopropionicum <400> 197 Met Glu Lys Val Phe Phe Val Asp Phe Asp Gly Thr Val Thr Lys Lys 1 5 10 15 Asp Thr Cys Val Ala Met Ile Glu Ala Phe Ala Gly Gly Asn Trp Arg 20 25 30 Glu Ile Asn Glu Ala Trp Glu Arg Lys Glu Ile Ser Thr Glu Glu Cys 35 40 45 Ala Asn Met Ile Phe Arg Leu Phe Arg Ala Gly Ile Glu Asp Ile Arg 50 55 60 Lys Leu Leu Asp Gly Ile Glu Ile Asp Gly His Phe Lys Asp Phe Leu 65 70 75 80 Ser Phe Cys Arg Glu Arg Gly Tyr Lys Ile Tyr Ile Leu Ser Asp Gly 85 90 95 Tyr Asp Phe Cys Ile Glu Thr Val Phe Lys Lys His Gly Ile Glu Leu 100 105 110 Pro Tyr Tyr Ala Asn Lys Met Val Tyr Gly Asn Gly Phe Lys Ile Glu 115 120 125 Cys Phe Arg Pro Asn Pro Ala Cys Gly Ile Cys Gly Thr Cys Lys Thr 130 135 140 Lys Leu Ile Glu Glu Leu Lys Gly Asp Gly Ser Gln Val Ile Tyr Ile 145 150 155 160 Gly Asp Gly Tyr Ser Asp Thr Cys Pro Ala Met Lys Ala Asp Val Val 165 170 175 Phe Ala Lys Gly Val Leu Tyr Arg His Cys Arg Glu Asn Gly Lys Lys 180 185 190 Ala Ile Tyr Tyr Asn Asn Phe Gly Asp Ile Ile Asn Tyr Phe Phe Gln 195 200 205 Ile Lys Lys Ser Leu 210 <210> 198 <211> 651 <212> DNA <213> Desulfotomaculum ruminis <220> <221> CDS <222> (1)..(651) <223> Desulfotomaculum ruminis gene encoding TMP phosphatase [WP_013840216] <400> 198 atg gaa acc att ctt ttt ctg gat ttt gac ggc acc att acc gag cag 48 Met Glu Thr Ile Leu Phe Leu Asp Phe Asp Gly Thr Ile Thr Glu Gln 1 5 10 15 gat acc tgc gat atg ctg atg gag cgc tac ggc aat gcg gaa tgt ctg 96 Asp Thr Cys Asp Met Leu Met Glu Arg Tyr Gly Asn Ala Glu Cys Leu 20 25 30 gaa ttg aac cgg cgc tgg gaa cgc aag gaa att tcc acc atg gaa tgt 144 Glu Leu Asn Arg Arg Trp Glu Arg Lys Glu Ile Ser Thr Met Glu Cys 35 40 45 gcc cgg cag tcc ttc cgg caa atg cag gta act ccc gag gtt cta aag 192 Ala Arg Gln Ser Phe Arg Gln Met Gln Val Thr Pro Glu Val Leu Lys 50 55 60 cgg ttg gtg cag gag gtg aag gta gac cct cat ttg aaa gaa ttg ctc 240 Arg Leu Val Gln Glu Val Lys Val Asp Pro His Leu Lys Glu Leu Leu 65 70 75 80 cgt ttc tgt gag cag gag aat tac ccc gcc tat att ttg agc gat ggg 288 Arg Phe Cys Glu Gln Glu Asn Tyr Pro Ala Tyr Ile Leu Ser Asp Gly 85 90 95 tat gaa ccc atc att cag ggg gta ctg cag cgg gaa gga ata aaa ata 336 Tyr Glu Pro Ile Ile Gln Gly Val Leu Gln Arg Glu Gly Ile Lys Ile 100 105 110 tct tgt ttt tgc aac ggg ttg tcc ttt gac ggc cag tac cgg gtc atg 384 Ser Cys Phe Cys Asn Gly Leu Ser Phe Asp Gly Gln Tyr Arg Val Met 115 120 125 gcg cct cac tat aat ccc cgg tgc ggc cgg tgc gga acc tgt aaa caa 432 Ala Pro His Tyr Asn Pro Arg Cys Gly Arg Cys Gly Thr Cys Lys Gln 130 135 140 aag ctg gtg gaa cgc ctg ggt cag ccg ggc gcc cgg aag att ttt gtg 480 Lys Leu Val Glu Arg Leu Gly Gln Pro Gly Ala Arg Lys Ile Phe Val 145 150 155 160 gga gac ggt tat tcg gat ttc tgt gcc gca gag tcc tgc agt aag gtc 528 Gly Asp Gly Tyr Ser Asp Phe Cys Ala Ala Glu Ser Cys Ser Lys Val 165 170 175 ttt gct aaa aaa aat tta ttg aag tat tgc ctg gaa aac cag att ccg 576 Phe Ala Lys Lys Asn Leu Leu Lys Tyr Cys Leu Glu Asn Gln Ile Pro 180 185 190 gcc cac ccc tat gaa acc ctg gga gag gtt tta cag tgg ctg aga gga 624 Ala His Pro Tyr Glu Thr Leu Gly Glu Val Leu Gln Trp Leu Arg Gly 195 200 205 gag gct gaa cat gga cat ccg gtt taa 651 Glu Ala Glu His Gly His Pro Val 210 215 <210> 199 <211> 216 <212> PRT <213> Desulfotomaculum ruminis <400> 199 Met Glu Thr Ile Leu Phe Leu Asp Phe Asp Gly Thr Ile Thr Glu Gln 1 5 10 15 Asp Thr Cys Asp Met Leu Met Glu Arg Tyr Gly Asn Ala Glu Cys Leu 20 25 30 Glu Leu Asn Arg Arg Trp Glu Arg Lys Glu Ile Ser Thr Met Glu Cys 35 40 45 Ala Arg Gln Ser Phe Arg Gln Met Gln Val Thr Pro Glu Val Leu Lys 50 55 60 Arg Leu Val Gln Glu Val Lys Val Asp Pro His Leu Lys Glu Leu Leu 65 70 75 80 Arg Phe Cys Glu Gln Glu Asn Tyr Pro Ala Tyr Ile Leu Ser Asp Gly 85 90 95 Tyr Glu Pro Ile Ile Gln Gly Val Leu Gln Arg Glu Gly Ile Lys Ile 100 105 110 Ser Cys Phe Cys Asn Gly Leu Ser Phe Asp Gly Gln Tyr Arg Val Met 115 120 125 Ala Pro His Tyr Asn Pro Arg Cys Gly Arg Cys Gly Thr Cys Lys Gln 130 135 140 Lys Leu Val Glu Arg Leu Gly Gln Pro Gly Ala Arg Lys Ile Phe Val 145 150 155 160 Gly Asp Gly Tyr Ser Asp Phe Cys Ala Ala Glu Ser Cys Ser Lys Val 165 170 175 Phe Ala Lys Lys Asn Leu Leu Lys Tyr Cys Leu Glu Asn Gln Ile Pro 180 185 190 Ala His Pro Tyr Glu Thr Leu Gly Glu Val Leu Gln Trp Leu Arg Gly 195 200 205 Glu Ala Glu His Gly His Pro Val 210 215 <210> 200 <211> 1896 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(1896) <223> ThiC gene from E. coli encoding HMP-P synthase <400> 200 atg tct gca aca aaa ctg acc cgc cgc gaa caa cgc gcc cgg gcc caa 48 Met Ser Ala Thr Lys Leu Thr Arg Arg Glu Gln Arg Ala Arg Ala Gln 1 5 10 15 cat ttt atc gac acc ctg gaa ggc acc gcc ttt ccc aac tca aaa cgc 96 His Phe Ile Asp Thr Leu Glu Gly Thr Ala Phe Pro Asn Ser Lys Arg 20 25 30 att tat atc act ggc aca cac ccc ggc gtg cgc gtg ccg atg cgt gag 144 Ile Tyr Ile Thr Gly Thr His Pro Gly Val Arg Val Pro Met Arg Glu 35 40 45 atc cag ctt agc ccg acg cta att ggc ggt agc aaa gaa cag ccg cag 192 Ile Gln Leu Ser Pro Thr Leu Ile Gly Gly Ser Lys Glu Gln Pro Gln 50 55 60 tac gaa gaa aac gaa gcg att ccg gtc tac gac acc tcc ggc ccg tat 240 Tyr Glu Glu Asn Glu Ala Ile Pro Val Tyr Asp Thr Ser Gly Pro Tyr 65 70 75 80 ggt gat ccg cag att gcc att aac gtg cag caa ggg ctg gca aaa cta 288 Gly Asp Pro Gln Ile Ala Ile Asn Val Gln Gln Gly Leu Ala Lys Leu 85 90 95 cgc cag ccg tgg atc gat gcg cgc ggc gat acc gaa gaa ctt acc gtg 336 Arg Gln Pro Trp Ile Asp Ala Arg Gly Asp Thr Glu Glu Leu Thr Val 100 105 110 cgc agt tcc gat tac act aaa gcg cgg ctg gca gat gat ggc ctc gac 384 Arg Ser Ser Asp Tyr Thr Lys Ala Arg Leu Ala Asp Asp Gly Leu Asp 115 120 125 gaa ctg cgt ttt agc ggc gta cta aca cca aaa cgc gcc aaa gca gga 432 Glu Leu Arg Phe Ser Gly Val Leu Thr Pro Lys Arg Ala Lys Ala Gly 130 135 140 cgc cgt gtc acc caa ctg cac tac gcc cgc cag ggc atc atc acg ccg 480 Arg Arg Val Thr Gln Leu His Tyr Ala Arg Gln Gly Ile Ile Thr Pro 145 150 155 160 gaa atg gaa ttc atc gcc atc cgc gag aat atg ggc cgc gag cgc atc 528 Glu Met Glu Phe Ile Ala Ile Arg Glu Asn Met Gly Arg Glu Arg Ile 165 170 175 cgt agc gag gtt tta cgc cac cag cat ccg gga atg agc ttt ggc gca 576 Arg Ser Glu Val Leu Arg His Gln His Pro Gly Met Ser Phe Gly Ala 180 185 190 cat ctg ccg gaa aat atc act gcg gaa ttt gtc cgt gat gaa gtt gct 624 His Leu Pro Glu Asn Ile Thr Ala Glu Phe Val Arg Asp Glu Val Ala 195 200 205 gcc gga cgt gcg att atc ccg gcc aac att aat cat ccg gaa tcg gag 672 Ala Gly Arg Ala Ile Ile Pro Ala Asn Ile Asn His Pro Glu Ser Glu 210 215 220 ccg atg att att ggt cgc aat ttc ctg gta aaa gtt aac gcc aat atc 720 Pro Met Ile Ile Gly Arg Asn Phe Leu Val Lys Val Asn Ala Asn Ile 225 230 235 240 ggc aac tcg gcg gtc acc tct tcc atc gaa gaa gaa gtg gaa aag ctg 768 Gly Asn Ser Ala Val Thr Ser Ser Ile Glu Glu Glu Val Glu Lys Leu 245 250 255 gta tgg tcc acg cgc tgg gga gcg gat acg gtg atg gat ctc tcc acc 816 Val Trp Ser Thr Arg Trp Gly Ala Asp Thr Val Met Asp Leu Ser Thr 260 265 270 ggt cgc tat att cac gaa acc cgc gag tgg att ttg cgt aac agc ccg 864 Gly Arg Tyr Ile His Glu Thr Arg Glu Trp Ile Leu Arg Asn Ser Pro 275 280 285 gtg ccg atc ggt aca gtg ccg atc tac cag gcg ctg gag aag gtt aac 912 Val Pro Ile Gly Thr Val Pro Ile Tyr Gln Ala Leu Glu Lys Val Asn 290 295 300 ggg atc gcc gaa gat ctt acc tgg gaa gcg ttc cgc gac acg ctg ctg 960 Gly Ile Ala Glu Asp Leu Thr Trp Glu Ala Phe Arg Asp Thr Leu Leu 305 310 315 320 gaa cag gcc gag caa ggt gtg gat tac ttc act atc cat gcg ggc gta 1008 Glu Gln Ala Glu Gln Gly Val Asp Tyr Phe Thr Ile His Ala Gly Val 325 330 335 ctg ctg cgc tat gtg ccg atg acc gcg aaa cgc ctg acc ggt atc gtc 1056 Leu Leu Arg Tyr Val Pro Met Thr Ala Lys Arg Leu Thr Gly Ile Val 340 345 350 tct cgc ggc ggt tcg att atg gcg aaa tgg tgc ctc tcc cat cat cag 1104 Ser Arg Gly Gly Ser Ile Met Ala Lys Trp Cys Leu Ser His His Gln 355 360 365 gaa aat ttc ctc tat caa cac ttc cgc gaa att tgt gaa atc tgt gcc 1152 Glu Asn Phe Leu Tyr Gln His Phe Arg Glu Ile Cys Glu Ile Cys Ala 370 375 380 gct tat gac gtt tcg ctg tcg ctg ggc gac ggt ctg cgc ccc ggt tct 1200 Ala Tyr Asp Val Ser Leu Ser Leu Gly Asp Gly Leu Arg Pro Gly Ser 385 390 395 400 att cag gac gcc aac gat gaa gcg cag ttt gcc gag ctg cat acg ctg 1248 Ile Gln Asp Ala Asn Asp Glu Ala Gln Phe Ala Glu Leu His Thr Leu 405 410 415 ggc gaa ctg acc aaa att gcc tgg gaa tat gac gtg cag gtg atg att 1296 Gly Glu Leu Thr Lys Ile Ala Trp Glu Tyr Asp Val Gln Val Met Ile 420 425 430 gaa ggc cca ggc cac gtg ccg atg cag atg atc cgc cgc aat atg acc 1344 Glu Gly Pro Gly His Val Pro Met Gln Met Ile Arg Arg Asn Met Thr 435 440 445 gag gag tta gag cac tgc cac gaa gcg ccg ttt tac act ctg ggg ccg 1392 Glu Glu Leu Glu His Cys His Glu Ala Pro Phe Tyr Thr Leu Gly Pro 450 455 460 cta act acc gat att gcg ccg ggc tat gac cac ttc acg tcg ggg att 1440 Leu Thr Thr Asp Ile Ala Pro Gly Tyr Asp His Phe Thr Ser Gly Ile 465 470 475 480 ggt gcg gcg atg att ggc tgg ttt ggc tgc gcg atg ctc tgt tac gta 1488 Gly Ala Ala Met Ile Gly Trp Phe Gly Cys Ala Met Leu Cys Tyr Val 485 490 495 acg cca aaa gag cat ctg ggt ctg ccc aat aaa gaa gat gtt aag cag 1536 Thr Pro Lys Glu His Leu Gly Leu Pro Asn Lys Glu Asp Val Lys Gln 500 505 510 ggg ctt atc acc tat aag att gct gcc cac gcc gct gac ctg gcg aaa 1584 Gly Leu Ile Thr Tyr Lys Ile Ala Ala His Ala Ala Asp Leu Ala Lys 515 520 525 ggg cat ccg ggc gcg caa att cgc gat aac gcc atg tcg aaa gcc cgc 1632 Gly His Pro Gly Ala Gln Ile Arg Asp Asn Ala Met Ser Lys Ala Arg 530 535 540 ttc gaa ttt cgc tgg gaa gac cag ttt aat ctg gcc ctc gac ccg ttt 1680 Phe Glu Phe Arg Trp Glu Asp Gln Phe Asn Leu Ala Leu Asp Pro Phe 545 550 555 560 acc gcc cgc gct tat cac gat gaa acc ctg ccg caa gag tca ggt aaa 1728 Thr Ala Arg Ala Tyr His Asp Glu Thr Leu Pro Gln Glu Ser Gly Lys 565 570 575 gtc gcc cat ttt tgc tcc atg tgt ggg ccg aaa ttc tgc tcg atg aaa 1776 Val Ala His Phe Cys Ser Met Cys Gly Pro Lys Phe Cys Ser Met Lys 580 585 590 atc agc cag gaa gtg cgt gat tac gcc gcc acg caa act att gaa atg 1824 Ile Ser Gln Glu Val Arg Asp Tyr Ala Ala Thr Gln Thr Ile Glu Met 595 600 605 gga atg gcg gat atg tcg gag aac ttc cgt gcc aga ggc gga gaa atc 1872 Gly Met Ala Asp Met Ser Glu Asn Phe Arg Ala Arg Gly Gly Glu Ile 610 615 620 tac ctg cgt aag gag gaa gcg tga 1896 Tyr Leu Arg Lys Glu Glu Ala 625 630 <210> 201 <211> 631 <212> PRT <213> Escherichia coli <400> 201 Met Ser Ala Thr Lys Leu Thr Arg Arg Glu Gln Arg Ala Arg Ala Gln 1 5 10 15 His Phe Ile Asp Thr Leu Glu Gly Thr Ala Phe Pro Asn Ser Lys Arg 20 25 30 Ile Tyr Ile Thr Gly Thr His Pro Gly Val Arg Val Pro Met Arg Glu 35 40 45 Ile Gln Leu Ser Pro Thr Leu Ile Gly Gly Ser Lys Glu Gln Pro Gln 50 55 60 Tyr Glu Glu Asn Glu Ala Ile Pro Val Tyr Asp Thr Ser Gly Pro Tyr 65 70 75 80 Gly Asp Pro Gln Ile Ala Ile Asn Val Gln Gln Gly Leu Ala Lys Leu 85 90 95 Arg Gln Pro Trp Ile Asp Ala Arg Gly Asp Thr Glu Glu Leu Thr Val 100 105 110 Arg Ser Ser Asp Tyr Thr Lys Ala Arg Leu Ala Asp Asp Gly Leu Asp 115 120 125 Glu Leu Arg Phe Ser Gly Val Leu Thr Pro Lys Arg Ala Lys Ala Gly 130 135 140 Arg Arg Val Thr Gln Leu His Tyr Ala Arg Gln Gly Ile Ile Thr Pro 145 150 155 160 Glu Met Glu Phe Ile Ala Ile Arg Glu Asn Met Gly Arg Glu Arg Ile 165 170 175 Arg Ser Glu Val Leu Arg His Gln His Pro Gly Met Ser Phe Gly Ala 180 185 190 His Leu Pro Glu Asn Ile Thr Ala Glu Phe Val Arg Asp Glu Val Ala 195 200 205 Ala Gly Arg Ala Ile Ile Pro Ala Asn Ile Asn His Pro Glu Ser Glu 210 215 220 Pro Met Ile Ile Gly Arg Asn Phe Leu Val Lys Val Asn Ala Asn Ile 225 230 235 240 Gly Asn Ser Ala Val Thr Ser Ser Ile Glu Glu Glu Val Glu Lys Leu 245 250 255 Val Trp Ser Thr Arg Trp Gly Ala Asp Thr Val Met Asp Leu Ser Thr 260 265 270 Gly Arg Tyr Ile His Glu Thr Arg Glu Trp Ile Leu Arg Asn Ser Pro 275 280 285 Val Pro Ile Gly Thr Val Pro Ile Tyr Gln Ala Leu Glu Lys Val Asn 290 295 300 Gly Ile Ala Glu Asp Leu Thr Trp Glu Ala Phe Arg Asp Thr Leu Leu 305 310 315 320 Glu Gln Ala Glu Gln Gly Val Asp Tyr Phe Thr Ile His Ala Gly Val 325 330 335 Leu Leu Arg Tyr Val Pro Met Thr Ala Lys Arg Leu Thr Gly Ile Val 340 345 350 Ser Arg Gly Gly Ser Ile Met Ala Lys Trp Cys Leu Ser His His Gln 355 360 365 Glu Asn Phe Leu Tyr Gln His Phe Arg Glu Ile Cys Glu Ile Cys Ala 370 375 380 Ala Tyr Asp Val Ser Leu Ser Leu Gly Asp Gly Leu Arg Pro Gly Ser 385 390 395 400 Ile Gln Asp Ala Asn Asp Glu Ala Gln Phe Ala Glu Leu His Thr Leu 405 410 415 Gly Glu Leu Thr Lys Ile Ala Trp Glu Tyr Asp Val Gln Val Met Ile 420 425 430 Glu Gly Pro Gly His Val Pro Met Gln Met Ile Arg Arg Asn Met Thr 435 440 445 Glu Glu Leu Glu His Cys His Glu Ala Pro Phe Tyr Thr Leu Gly Pro 450 455 460 Leu Thr Thr Asp Ile Ala Pro Gly Tyr Asp His Phe Thr Ser Gly Ile 465 470 475 480 Gly Ala Ala Met Ile Gly Trp Phe Gly Cys Ala Met Leu Cys Tyr Val 485 490 495 Thr Pro Lys Glu His Leu Gly Leu Pro Asn Lys Glu Asp Val Lys Gln 500 505 510 Gly Leu Ile Thr Tyr Lys Ile Ala Ala His Ala Ala Asp Leu Ala Lys 515 520 525 Gly His Pro Gly Ala Gln Ile Arg Asp Asn Ala Met Ser Lys Ala Arg 530 535 540 Phe Glu Phe Arg Trp Glu Asp Gln Phe Asn Leu Ala Leu Asp Pro Phe 545 550 555 560 Thr Ala Arg Ala Tyr His Asp Glu Thr Leu Pro Gln Glu Ser Gly Lys 565 570 575 Val Ala His Phe Cys Ser Met Cys Gly Pro Lys Phe Cys Ser Met Lys 580 585 590 Ile Ser Gln Glu Val Arg Asp Tyr Ala Ala Thr Gln Thr Ile Glu Met 595 600 605 Gly Met Ala Asp Met Ser Glu Asn Phe Arg Ala Arg Gly Gly Glu Ile 610 615 620 Tyr Leu Arg Lys Glu Glu Ala 625 630 <210> 202 <211> 1371 <212> DNA <213> Synechococcus elongatus <220> <221> CDS <222> (1)..(1371) <223> ThiC gene from Synechococcus_elongatus_PCC_7942:_[NC_007604] encoding a HMP-P synthase <400> 202 atg cgc agc gac tgg atc gca ccc cgc cga ggc caa gcc aac gtc act 48 Met Arg Ser Asp Trp Ile Ala Pro Arg Arg Gly Gln Ala Asn Val Thr 1 5 10 15 caa atg cac tac gcc cgc caa ggc gtg atc acc gaa gaa atg gac ttc 96 Gln Met His Tyr Ala Arg Gln Gly Val Ile Thr Glu Glu Met Asp Phe 20 25 30 gtg gcg cgg cgc gaa aat ctg cca gcc gat cta att cgg gat gaa gtg 144 Val Ala Arg Arg Glu Asn Leu Pro Ala Asp Leu Ile Arg Asp Glu Val 35 40 45 gca cgg ggt cgg atg att atc ccc gcc aac atc aac cac acc aat ttg 192 Ala Arg Gly Arg Met Ile Ile Pro Ala Asn Ile Asn His Thr Asn Leu 50 55 60 gag ccg atg gcg atc ggc att gcc tcc aag tgc aag gtc aac gcc aac 240 Glu Pro Met Ala Ile Gly Ile Ala Ser Lys Cys Lys Val Asn Ala Asn 65 70 75 80 atc ggt gct tcg cct aac gcc tcc aac atc gat gaa gaa gtc gag aag 288 Ile Gly Ala Ser Pro Asn Ala Ser Asn Ile Asp Glu Glu Val Glu Lys 85 90 95 ctg aag ctc gcg gtc aaa tac ggt gcc gat acc gtc atg gac ctc tcg 336 Leu Lys Leu Ala Val Lys Tyr Gly Ala Asp Thr Val Met Asp Leu Ser 100 105 110 acc ggc ggc ggc aac ctc gat gag att cgc acc gcg atc atc aat gct 384 Thr Gly Gly Gly Asn Leu Asp Glu Ile Arg Thr Ala Ile Ile Asn Ala 115 120 125 tcg ccg gta ccg atc ggc acc gtg ccg gtc tac caa gcc ctg gaa tcc 432 Ser Pro Val Pro Ile Gly Thr Val Pro Val Tyr Gln Ala Leu Glu Ser 130 135 140 gtt cac ggg cgc atc gaa aaa ctc agc gcc gac gac ttc ttg cat gtg 480 Val His Gly Arg Ile Glu Lys Leu Ser Ala Asp Asp Phe Leu His Val 145 150 155 160 atc gaa aag cac tgc gaa cag ggc gtc gac tac caa acc atc cac gcc 528 Ile Glu Lys His Cys Glu Gln Gly Val Asp Tyr Gln Thr Ile His Ala 165 170 175 ggt ctg ctg att gaa cac ctg ccc aag gtc aag agc cgg atc acc ggg 576 Gly Leu Leu Ile Glu His Leu Pro Lys Val Lys Ser Arg Ile Thr Gly 180 185 190 att gtt tcg cgg ggc ggc ggc atc att gcc cag tgg atg ctc tac cac 624 Ile Val Ser Arg Gly Gly Gly Ile Ile Ala Gln Trp Met Leu Tyr His 195 200 205 cac aag caa aac ccg ctc tat acc cac ttt cgc gac atc atc gaa atc 672 His Lys Gln Asn Pro Leu Tyr Thr His Phe Arg Asp Ile Ile Glu Ile 210 215 220 ttc aag cgc tac gac tgt agc ttc agc ttg ggt gac tcg ctg cgg ccg 720 Phe Lys Arg Tyr Asp Cys Ser Phe Ser Leu Gly Asp Ser Leu Arg Pro 225 230 235 240 ggt tgc ctg cac gat gct agc gac gat gcc cag ctc agc gag ctg aag 768 Gly Cys Leu His Asp Ala Ser Asp Asp Ala Gln Leu Ser Glu Leu Lys 245 250 255 act ctc ggt caa ctg acg cgg gtt gct tgg gaa cac gac gtg caa gtc 816 Thr Leu Gly Gln Leu Thr Arg Val Ala Trp Glu His Asp Val Gln Val 260 265 270 atg gtc gaa ggg cca ggc cac gtt ccc atg gac cag atc gag ttc aac 864 Met Val Glu Gly Pro Gly His Val Pro Met Asp Gln Ile Glu Phe Asn 275 280 285 gtc cgc aag caa atg gaa gag tgc tca gaa gct ccc ttc tac gtc ttg 912 Val Arg Lys Gln Met Glu Glu Cys Ser Glu Ala Pro Phe Tyr Val Leu 290 295 300 ggt ccc ctc gtg acc gac att gca ccg ggc tat gac cac atc acc agc 960 Gly Pro Leu Val Thr Asp Ile Ala Pro Gly Tyr Asp His Ile Thr Ser 305 310 315 320 gcg atc ggg gca gca atg gcg ggc tgg tat ggc acg gca atg ctc tgc 1008 Ala Ile Gly Ala Ala Met Ala Gly Trp Tyr Gly Thr Ala Met Leu Cys 325 330 335 tac gtc acg ccc aaa gag cac ttg ggt ctg ccc aat gcg gaa gat gtg 1056 Tyr Val Thr Pro Lys Glu His Leu Gly Leu Pro Asn Ala Glu Asp Val 340 345 350 cgc aat ggt ttg atc gcc tac aaa att gcg gct cat gca gca gat atc 1104 Arg Asn Gly Leu Ile Ala Tyr Lys Ile Ala Ala His Ala Ala Asp Ile 355 360 365 gct cgc cac cgt ccg ggt gct cgc gat cgc gat gat gaa ctg agt cgg 1152 Ala Arg His Arg Pro Gly Ala Arg Asp Arg Asp Asp Glu Leu Ser Arg 370 375 380 gca cgc tac gcc ttc gac tgg aac aag caa ttt gac ttg agc ctc gat 1200 Ala Arg Tyr Ala Phe Asp Trp Asn Lys Gln Phe Asp Leu Ser Leu Asp 385 390 395 400 cca gag cgg gcg cgg gaa tac cac gac gaa act ctg cca gca gat atc 1248 Pro Glu Arg Ala Arg Glu Tyr His Asp Glu Thr Leu Pro Ala Asp Ile 405 410 415 tac aaa acg gca gaa ttc tgt tcg atg tgt gga ccg aag cac tgt ccg 1296 Tyr Lys Thr Ala Glu Phe Cys Ser Met Cys Gly Pro Lys His Cys Pro 420 425 430 atg caa acc aag atc acc gag gaa gat cta acc gag ttg gaa aaa ttc 1344 Met Gln Thr Lys Ile Thr Glu Glu Asp Leu Thr Glu Leu Glu Lys Phe 435 440 445 ctc gag aaa gat agc gct ctg gcg tag 1371 Leu Glu Lys Asp Ser Ala Leu Ala 450 455 <210> 203 <211> 456 <212> PRT <213> Synechococcus elongatus <400> 203 Met Arg Ser Asp Trp Ile Ala Pro Arg Arg Gly Gln Ala Asn Val Thr 1 5 10 15 Gln Met His Tyr Ala Arg Gln Gly Val Ile Thr Glu Glu Met Asp Phe 20 25 30 Val Ala Arg Arg Glu Asn Leu Pro Ala Asp Leu Ile Arg Asp Glu Val 35 40 45 Ala Arg Gly Arg Met Ile Ile Pro Ala Asn Ile Asn His Thr Asn Leu 50 55 60 Glu Pro Met Ala Ile Gly Ile Ala Ser Lys Cys Lys Val Asn Ala Asn 65 70 75 80 Ile Gly Ala Ser Pro Asn Ala Ser Asn Ile Asp Glu Glu Val Glu Lys 85 90 95 Leu Lys Leu Ala Val Lys Tyr Gly Ala Asp Thr Val Met Asp Leu Ser 100 105 110 Thr Gly Gly Gly Asn Leu Asp Glu Ile Arg Thr Ala Ile Ile Asn Ala 115 120 125 Ser Pro Val Pro Ile Gly Thr Val Pro Val Tyr Gln Ala Leu Glu Ser 130 135 140 Val His Gly Arg Ile Glu Lys Leu Ser Ala Asp Asp Phe Leu His Val 145 150 155 160 Ile Glu Lys His Cys Glu Gln Gly Val Asp Tyr Gln Thr Ile His Ala 165 170 175 Gly Leu Leu Ile Glu His Leu Pro Lys Val Lys Ser Arg Ile Thr Gly 180 185 190 Ile Val Ser Arg Gly Gly Gly Ile Ile Ala Gln Trp Met Leu Tyr His 195 200 205 His Lys Gln Asn Pro Leu Tyr Thr His Phe Arg Asp Ile Ile Glu Ile 210 215 220 Phe Lys Arg Tyr Asp Cys Ser Phe Ser Leu Gly Asp Ser Leu Arg Pro 225 230 235 240 Gly Cys Leu His Asp Ala Ser Asp Asp Ala Gln Leu Ser Glu Leu Lys 245 250 255 Thr Leu Gly Gln Leu Thr Arg Val Ala Trp Glu His Asp Val Gln Val 260 265 270 Met Val Glu Gly Pro Gly His Val Pro Met Asp Gln Ile Glu Phe Asn 275 280 285 Val Arg Lys Gln Met Glu Glu Cys Ser Glu Ala Pro Phe Tyr Val Leu 290 295 300 Gly Pro Leu Val Thr Asp Ile Ala Pro Gly Tyr Asp His Ile Thr Ser 305 310 315 320 Ala Ile Gly Ala Ala Met Ala Gly Trp Tyr Gly Thr Ala Met Leu Cys 325 330 335 Tyr Val Thr Pro Lys Glu His Leu Gly Leu Pro Asn Ala Glu Asp Val 340 345 350 Arg Asn Gly Leu Ile Ala Tyr Lys Ile Ala Ala His Ala Ala Asp Ile 355 360 365 Ala Arg His Arg Pro Gly Ala Arg Asp Arg Asp Asp Glu Leu Ser Arg 370 375 380 Ala Arg Tyr Ala Phe Asp Trp Asn Lys Gln Phe Asp Leu Ser Leu Asp 385 390 395 400 Pro Glu Arg Ala Arg Glu Tyr His Asp Glu Thr Leu Pro Ala Asp Ile 405 410 415 Tyr Lys Thr Ala Glu Phe Cys Ser Met Cys Gly Pro Lys His Cys Pro 420 425 430 Met Gln Thr Lys Ile Thr Glu Glu Asp Leu Thr Glu Leu Glu Lys Phe 435 440 445 Leu Glu Lys Asp Ser Ala Leu Ala 450 455 <210> 204 <211> 1764 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(1764) <223> ThiC gene from Corynebacterium glutamicum encoding an HMP-P synthase <400> 204 atg acg cct acc caa aat gag atc cac ccg aaa cat agc tac tcc ccc 48 Met Thr Pro Thr Gln Asn Glu Ile His Pro Lys His Ser Tyr Ser Pro 1 5 10 15 atc cgc aag gac ggt ctc gag gtc ccg gag acc gaa atc cgc ctc gat 96 Ile Arg Lys Asp Gly Leu Glu Val Pro Glu Thr Glu Ile Arg Leu Asp 20 25 30 gac tcg cca agc ggc ccc aac gaa ccc ttc cgc atc tac cgc acc cgt 144 Asp Ser Pro Ser Gly Pro Asn Glu Pro Phe Arg Ile Tyr Arg Thr Arg 35 40 45 ggc cca gaa acc aac ccc aag cag gga ctt ccg cgg ctg cgc gag tca 192 Gly Pro Glu Thr Asn Pro Lys Gln Gly Leu Pro Arg Leu Arg Glu Ser 50 55 60 tgg atc acc gcc cgc ggc gac gtt gcc acc tat cag ggg cgc gag cgt 240 Trp Ile Thr Ala Arg Gly Asp Val Ala Thr Tyr Gln Gly Arg Glu Arg 65 70 75 80 ttg ctt atc gac gac ggc cgc tcg gca atg cgt cga ggt caa gct tcg 288 Leu Leu Ile Asp Asp Gly Arg Ser Ala Met Arg Arg Gly Gln Ala Ser 85 90 95 gct gag tgg aaa ggc caa aaa cca gct cct ttg aag gcg cta cct ggc 336 Ala Glu Trp Lys Gly Gln Lys Pro Ala Pro Leu Lys Ala Leu Pro Gly 100 105 110 aaa aga gtc acc caa atg gcc tat gca cgt gct ggc gtg att act cgt 384 Lys Arg Val Thr Gln Met Ala Tyr Ala Arg Ala Gly Val Ile Thr Arg 115 120 125 gaa atg gag ttt gta gcg ctg cgc gaa cac gtt gat gcg gag ttt gtg 432 Glu Met Glu Phe Val Ala Leu Arg Glu His Val Asp Ala Glu Phe Val 130 135 140 cgc tct gag gtg gcg cgc ggt cgg gcc att att ccc aac aac gtc aac 480 Arg Ser Glu Val Ala Arg Gly Arg Ala Ile Ile Pro Asn Asn Val Asn 145 150 155 160 cac ccc gaa tct gaa ccg atg att att ggt cgc aaa ttt ttg acc aaa 528 His Pro Glu Ser Glu Pro Met Ile Ile Gly Arg Lys Phe Leu Thr Lys 165 170 175 atc aac gcc aat att ggc aat tct gcg gtc acc tct tca atc gag gaa 576 Ile Asn Ala Asn Ile Gly Asn Ser Ala Val Thr Ser Ser Ile Glu Glu 180 185 190 gag gtg tcc aag ctg cag tgg gcc acg cgc tgg ggt gcc gat acc gtg 624 Glu Val Ser Lys Leu Gln Trp Ala Thr Arg Trp Gly Ala Asp Thr Val 195 200 205 atg gat cta tcc acc ggc gat gat att cac acc acc cgc gaa tgg att 672 Met Asp Leu Ser Thr Gly Asp Asp Ile His Thr Thr Arg Glu Trp Ile 210 215 220 atc cgc aac tcc ccc gtt cct atc ggc acc gtc ccg atc tac caa gcg 720 Ile Arg Asn Ser Pro Val Pro Ile Gly Thr Val Pro Ile Tyr Gln Ala 225 230 235 240 ctg gaa aaa gta aat ggc gtg gcc gca gac ctt aac tgg gaa gta ttc 768 Leu Glu Lys Val Asn Gly Val Ala Ala Asp Leu Asn Trp Glu Val Phe 245 250 255 cgc gat acc atc att gag cag tgt gaa caa ggc gtg gac tat atg acc 816 Arg Asp Thr Ile Ile Glu Gln Cys Glu Gln Gly Val Asp Tyr Met Thr 260 265 270 atc cac gcc ggc gtc ctg ctg gct tat atc cca ctg act acc cgt cgt 864 Ile His Ala Gly Val Leu Leu Ala Tyr Ile Pro Leu Thr Thr Arg Arg 275 280 285 gtc acc ggc att gtc tcc cgc ggc gga tcc att atg gcc ggt tgg tgt 912 Val Thr Gly Ile Val Ser Arg Gly Gly Ser Ile Met Ala Gly Trp Cys 290 295 300 ctg gcg cat cac cgc gaa tca ttc ctc tac gag cat ttc gac gag ctg 960 Leu Ala His His Arg Glu Ser Phe Leu Tyr Glu His Phe Asp Glu Leu 305 310 315 320 tgc gaa atc ttt gca caa tat gac gtc gca ttc tcc ctc ggt gat ggc 1008 Cys Glu Ile Phe Ala Gln Tyr Asp Val Ala Phe Ser Leu Gly Asp Gly 325 330 335 cta cgc ccc gga tcg ctt gcc gat gcc aac gac gcc gcg caa ttc gcc 1056 Leu Arg Pro Gly Ser Leu Ala Asp Ala Asn Asp Ala Ala Gln Phe Ala 340 345 350 gag ctg aaa acc att ggt gag ctc acc caa cgc gcc tgg gaa tac gat 1104 Glu Leu Lys Thr Ile Gly Glu Leu Thr Gln Arg Ala Trp Glu Tyr Asp 355 360 365 gta caa gta atg gtc gaa gga cct gga cac gtg cca cta aac atg atc 1152 Val Gln Val Met Val Glu Gly Pro Gly His Val Pro Leu Asn Met Ile 370 375 380 cag gaa aac aac gag ctg gaa caa aag tgg gca gcg gac gca cct ttt 1200 Gln Glu Asn Asn Glu Leu Glu Gln Lys Trp Ala Ala Asp Ala Pro Phe 385 390 395 400 tac act ctt gga cca cta gtt acc gac atc gct cca ggt tat gac cac 1248 Tyr Thr Leu Gly Pro Leu Val Thr Asp Ile Ala Pro Gly Tyr Asp His 405 410 415 atc act tct gcc att ggt gca gct cac atc gcc atg ggt ggc acc gcc 1296 Ile Thr Ser Ala Ile Gly Ala Ala His Ile Ala Met Gly Gly Thr Ala 420 425 430 atg ctg tgt tat gtc acc ccg aaa gaa cac ctt ggc ctg ccc aac cgt 1344 Met Leu Cys Tyr Val Thr Pro Lys Glu His Leu Gly Leu Pro Asn Arg 435 440 445 gac gac gtc aaa acc ggc gta atc acc tac aag ctc gct gcc cac gca 1392 Asp Asp Val Lys Thr Gly Val Ile Thr Tyr Lys Leu Ala Ala His Ala 450 455 460 gca gat gtg gcc aag ggt cat ccc ggc gcg cgt gcc tgg gac gac gcc 1440 Ala Asp Val Ala Lys Gly His Pro Gly Ala Arg Ala Trp Asp Asp Ala 465 470 475 480 atg agt aaa gcg cgt ttt gaa ttc cgt tgg aat gat cag ttt gcg ctc 1488 Met Ser Lys Ala Arg Phe Glu Phe Arg Trp Asn Asp Gln Phe Ala Leu 485 490 495 tcc ctc gac ccc gac act gca atc gct tat cac gac gaa acc ctg ccg 1536 Ser Leu Asp Pro Asp Thr Ala Ile Ala Tyr His Asp Glu Thr Leu Pro 500 505 510 gca gag cct gcg aaa acc gca cac ttc tgt tca atg tgt ggc ccg aag 1584 Ala Glu Pro Ala Lys Thr Ala His Phe Cys Ser Met Cys Gly Pro Lys 515 520 525 ttc tgc tcc atg cga att agc cag gac att cgc gat atg ttt ggc gat 1632 Phe Cys Ser Met Arg Ile Ser Gln Asp Ile Arg Asp Met Phe Gly Asp 530 535 540 caa atc gcg gaa ttg ggg atg cct ggg gtt ggg gat tct tct agt gct 1680 Gln Ile Ala Glu Leu Gly Met Pro Gly Val Gly Asp Ser Ser Ser Ala 545 550 555 560 gtt gct tct agt ggg gca cgg gag ggg atg gct gag aaa tcc cgg gaa 1728 Val Ala Ser Ser Gly Ala Arg Glu Gly Met Ala Glu Lys Ser Arg Glu 565 570 575 ttt att gct ggt ggt gcg gag gtt tat cgg cgt tag 1764 Phe Ile Ala Gly Gly Ala Glu Val Tyr Arg Arg 580 585 <210> 205 <211> 587 <212> PRT <213> Corynebacterium glutamicum <400> 205 Met Thr Pro Thr Gln Asn Glu Ile His Pro Lys His Ser Tyr Ser Pro 1 5 10 15 Ile Arg Lys Asp Gly Leu Glu Val Pro Glu Thr Glu Ile Arg Leu Asp 20 25 30 Asp Ser Pro Ser Gly Pro Asn Glu Pro Phe Arg Ile Tyr Arg Thr Arg 35 40 45 Gly Pro Glu Thr Asn Pro Lys Gln Gly Leu Pro Arg Leu Arg Glu Ser 50 55 60 Trp Ile Thr Ala Arg Gly Asp Val Ala Thr Tyr Gln Gly Arg Glu Arg 65 70 75 80 Leu Leu Ile Asp Asp Gly Arg Ser Ala Met Arg Arg Gly Gln Ala Ser 85 90 95 Ala Glu Trp Lys Gly Gln Lys Pro Ala Pro Leu Lys Ala Leu Pro Gly 100 105 110 Lys Arg Val Thr Gln Met Ala Tyr Ala Arg Ala Gly Val Ile Thr Arg 115 120 125 Glu Met Glu Phe Val Ala Leu Arg Glu His Val Asp Ala Glu Phe Val 130 135 140 Arg Ser Glu Val Ala Arg Gly Arg Ala Ile Ile Pro Asn Asn Val Asn 145 150 155 160 His Pro Glu Ser Glu Pro Met Ile Ile Gly Arg Lys Phe Leu Thr Lys 165 170 175 Ile Asn Ala Asn Ile Gly Asn Ser Ala Val Thr Ser Ser Ile Glu Glu 180 185 190 Glu Val Ser Lys Leu Gln Trp Ala Thr Arg Trp Gly Ala Asp Thr Val 195 200 205 Met Asp Leu Ser Thr Gly Asp Asp Ile His Thr Thr Arg Glu Trp Ile 210 215 220 Ile Arg Asn Ser Pro Val Pro Ile Gly Thr Val Pro Ile Tyr Gln Ala 225 230 235 240 Leu Glu Lys Val Asn Gly Val Ala Ala Asp Leu Asn Trp Glu Val Phe 245 250 255 Arg Asp Thr Ile Ile Glu Gln Cys Glu Gln Gly Val Asp Tyr Met Thr 260 265 270 Ile His Ala Gly Val Leu Leu Ala Tyr Ile Pro Leu Thr Thr Arg Arg 275 280 285 Val Thr Gly Ile Val Ser Arg Gly Gly Ser Ile Met Ala Gly Trp Cys 290 295 300 Leu Ala His His Arg Glu Ser Phe Leu Tyr Glu His Phe Asp Glu Leu 305 310 315 320 Cys Glu Ile Phe Ala Gln Tyr Asp Val Ala Phe Ser Leu Gly Asp Gly 325 330 335 Leu Arg Pro Gly Ser Leu Ala Asp Ala Asn Asp Ala Ala Gln Phe Ala 340 345 350 Glu Leu Lys Thr Ile Gly Glu Leu Thr Gln Arg Ala Trp Glu Tyr Asp 355 360 365 Val Gln Val Met Val Glu Gly Pro Gly His Val Pro Leu Asn Met Ile 370 375 380 Gln Glu Asn Asn Glu Leu Glu Gln Lys Trp Ala Ala Asp Ala Pro Phe 385 390 395 400 Tyr Thr Leu Gly Pro Leu Val Thr Asp Ile Ala Pro Gly Tyr Asp His 405 410 415 Ile Thr Ser Ala Ile Gly Ala Ala His Ile Ala Met Gly Gly Thr Ala 420 425 430 Met Leu Cys Tyr Val Thr Pro Lys Glu His Leu Gly Leu Pro Asn Arg 435 440 445 Asp Asp Val Lys Thr Gly Val Ile Thr Tyr Lys Leu Ala Ala His Ala 450 455 460 Ala Asp Val Ala Lys Gly His Pro Gly Ala Arg Ala Trp Asp Asp Ala 465 470 475 480 Met Ser Lys Ala Arg Phe Glu Phe Arg Trp Asn Asp Gln Phe Ala Leu 485 490 495 Ser Leu Asp Pro Asp Thr Ala Ile Ala Tyr His Asp Glu Thr Leu Pro 500 505 510 Ala Glu Pro Ala Lys Thr Ala His Phe Cys Ser Met Cys Gly Pro Lys 515 520 525 Phe Cys Ser Met Arg Ile Ser Gln Asp Ile Arg Asp Met Phe Gly Asp 530 535 540 Gln Ile Ala Glu Leu Gly Met Pro Gly Val Gly Asp Ser Ser Ser Ala 545 550 555 560 Val Ala Ser Ser Gly Ala Arg Glu Gly Met Ala Glu Lys Ser Arg Glu 565 570 575 Phe Ile Ala Gly Gly Ala Glu Val Tyr Arg Arg 580 585 <210> 206 <211> 1869 <212> DNA <213> Candidatus Baumannia cicadellinicola <220> <221> CDS <222> (1)..(1869) <223> ThiC gene from Candidatus Baumannia cicadellinicola [WP_011520252] encoding HMP-P synthase <400> 206 atg tca aga tca tca ata cct gct tca cgc cga gtg agc cgt gca aaa 48 Met Ser Arg Ser Ser Ile Pro Ala Ser Arg Arg Val Ser Arg Ala Lys 1 5 10 15 gca cag gct ttt atg gat agc tta aca ggt agt agc tat ttt cct aac 96 Ala Gln Ala Phe Met Asp Ser Leu Thr Gly Ser Ser Tyr Phe Pro Asn 20 25 30 tca aga agg ata tat tta caa ggt aaa aca cct tca gta cat gta cca 144 Ser Arg Arg Ile Tyr Leu Gln Gly Lys Thr Pro Ser Val His Val Pro 35 40 45 atg cgt gaa att aag cta cat cct aca ttg atc ggt aaa aac ggt gaa 192 Met Arg Glu Ile Lys Leu His Pro Thr Leu Ile Gly Lys Asn Gly Glu 50 55 60 cat tat gag gat aat caa cct ata cca gtt tat gat act tca ggt cct 240 His Tyr Glu Asp Asn Gln Pro Ile Pro Val Tyr Asp Thr Ser Gly Pro 65 70 75 80 tac ggt gat cct act ata gca att aac gta cgt aca ggt ctt aac cgg 288 Tyr Gly Asp Pro Thr Ile Ala Ile Asn Val Arg Thr Gly Leu Asn Arg 85 90 95 tta cgc gag ata tgg att ctt gca cga caa gat agt gag cca ata agt 336 Leu Arg Glu Ile Trp Ile Leu Ala Arg Gln Asp Ser Glu Pro Ile Ser 100 105 110 aat aat aat aac gat cgt cag agt tca gat aaa cag tta agt ttt act 384 Asn Asn Asn Asn Asp Arg Gln Ser Ser Asp Lys Gln Leu Ser Phe Thr 115 120 125 act aac tat aat cca cgc cga gct agc tat gga cgc tgt att aca caa 432 Thr Asn Tyr Asn Pro Arg Arg Ala Ser Tyr Gly Arg Cys Ile Thr Gln 130 135 140 tta cat tac gca cgt gcc ggt atc ata acg cca gaa atg gag ttt ata 480 Leu His Tyr Ala Arg Ala Gly Ile Ile Thr Pro Glu Met Glu Phe Ile 145 150 155 160 gct tta cgt gaa aat atg ggc cga gaa cgt att agt agc aac gtg cta 528 Ala Leu Arg Glu Asn Met Gly Arg Glu Arg Ile Ser Ser Asn Val Leu 165 170 175 cat cag cag cat tta ggt tct aac ttt ggt gct aaa aaa gct gat cat 576 His Gln Gln His Leu Gly Ser Asn Phe Gly Ala Lys Lys Ala Asp His 180 185 190 att aca gca gaa ttt gtc cgg cag gaa gta gca gca gga cgt gct att 624 Ile Thr Ala Glu Phe Val Arg Gln Glu Val Ala Ala Gly Arg Ala Ile 195 200 205 ata cct agt aat att aat cat cca gaa tct gag cca atg atc att ggc 672 Ile Pro Ser Asn Ile Asn His Pro Glu Ser Glu Pro Met Ile Ile Gly 210 215 220 cgt aat ttt ctc gta aaa gta aat gca aat att ggt aac tca gca gta 720 Arg Asn Phe Leu Val Lys Val Asn Ala Asn Ile Gly Asn Ser Ala Val 225 230 235 240 aca tct tct att gag gaa gaa gtc gaa aag tta gta tgg gct act cgt 768 Thr Ser Ser Ile Glu Glu Glu Val Glu Lys Leu Val Trp Ala Thr Arg 245 250 255 tgg gga gct gat aca gtc atg gac tta tct act ggt agt tat att cac 816 Trp Gly Ala Asp Thr Val Met Asp Leu Ser Thr Gly Ser Tyr Ile His 260 265 270 gaa act aga gaa tgg ata tta cgt aat agc cca gta cct ata ggt act 864 Glu Thr Arg Glu Trp Ile Leu Arg Asn Ser Pro Val Pro Ile Gly Thr 275 280 285 gta cct atc tat caa gcg tta gaa aaa gta aat gga gtc ata gaa aat 912 Val Pro Ile Tyr Gln Ala Leu Glu Lys Val Asn Gly Val Ile Glu Asn 290 295 300 ctt aat tgg gat att ttc tac gag aca tta tta gaa caa gct aac caa 960 Leu Asn Trp Asp Ile Phe Tyr Glu Thr Leu Leu Glu Gln Ala Asn Gln 305 310 315 320 gga gta gat tat ttt acg att cat gct ggc gta tta aaa cgt tat gtt 1008 Gly Val Asp Tyr Phe Thr Ile His Ala Gly Val Leu Lys Arg Tyr Val 325 330 335 cta cta aca gct agt agg tta act ggt atc gta tcg cgt ggt ggc tct 1056 Leu Leu Thr Ala Ser Arg Leu Thr Gly Ile Val Ser Arg Gly Gly Ser 340 345 350 att atg gct caa tgg agt tta gta cat aat cag gaa aac ttc ctt tat 1104 Ile Met Ala Gln Trp Ser Leu Val His Asn Gln Glu Asn Phe Leu Tyr 355 360 365 gag cat ttt agt gaa att tgc aag ctt tgt gct gct tat gat att gct 1152 Glu His Phe Ser Glu Ile Cys Lys Leu Cys Ala Ala Tyr Asp Ile Ala 370 375 380 cta tct ctt gga gat ggt cta aga ccc ggt tcc gta caa gat gct aat 1200 Leu Ser Leu Gly Asp Gly Leu Arg Pro Gly Ser Val Gln Asp Ala Asn 385 390 395 400 gat gaa gca caa ttt tct gag tta cat aca cta ggc gaa tta act aaa 1248 Asp Glu Ala Gln Phe Ser Glu Leu His Thr Leu Gly Glu Leu Thr Lys 405 410 415 att gcc tgg gaa tat gat gtg caa gta atg atc gaa gga cct ggt cat 1296 Ile Ala Trp Glu Tyr Asp Val Gln Val Met Ile Glu Gly Pro Gly His 420 425 430 att cca cta cat atg att gag cgt aat atg act gat caa ctt aaa tat 1344 Ile Pro Leu His Met Ile Glu Arg Asn Met Thr Asp Gln Leu Lys Tyr 435 440 445 tgc cac gaa gca cca ttc tac act ctc gga cca ctc aca aca gat att 1392 Cys His Glu Ala Pro Phe Tyr Thr Leu Gly Pro Leu Thr Thr Asp Ile 450 455 460 gct cct ggt tat gac cac ttt act tca ggt att ggt gcc gca cta ata 1440 Ala Pro Gly Tyr Asp His Phe Thr Ser Gly Ile Gly Ala Ala Leu Ile 465 470 475 480 ggc tgg ttt gga tgt gct atg ctg tgc tat gta act cct aaa gag cat 1488 Gly Trp Phe Gly Cys Ala Met Leu Cys Tyr Val Thr Pro Lys Glu His 485 490 495 cta ggt tta cct aat aag gaa gac gta aaa cag ggt tta att gcc tat 1536 Leu Gly Leu Pro Asn Lys Glu Asp Val Lys Gln Gly Leu Ile Ala Tyr 500 505 510 aaa att gcc gca cat gct gca gat cta gct aaa gga cat cct ggt gct 1584 Lys Ile Ala Ala His Ala Ala Asp Leu Ala Lys Gly His Pro Gly Ala 515 520 525 caa ata cgt gat aat gct atg tca aaa gct cgt ttc gaa ttt cgc tgg 1632 Gln Ile Arg Asp Asn Ala Met Ser Lys Ala Arg Phe Glu Phe Arg Trp 530 535 540 gaa gat caa ttt aac tta gct tta gat cct ttt acg gcg cgt atg tat 1680 Glu Asp Gln Phe Asn Leu Ala Leu Asp Pro Phe Thr Ala Arg Met Tyr 545 550 555 560 cac gat gaa act ata ccg caa aca gca gga aaa tta gca aat ttt tgc 1728 His Asp Glu Thr Ile Pro Gln Thr Ala Gly Lys Leu Ala Asn Phe Cys 565 570 575 tcg atg tgt ggt cct aag ttt tgt tct atg aag cta tca aaa aaa ata 1776 Ser Met Cys Gly Pro Lys Phe Cys Ser Met Lys Leu Ser Lys Lys Ile 580 585 590 cgt aat tac act aat atg aaa aat ata aaa act att agt aat agt ttc 1824 Arg Asn Tyr Thr Asn Met Lys Asn Ile Lys Thr Ile Ser Asn Ser Phe 595 600 605 atg aat aaa tta gat aat agc ggt att aaa aat gct gac cga taa 1869 Met Asn Lys Leu Asp Asn Ser Gly Ile Lys Asn Ala Asp Arg 610 615 620 <210> 207 <211> 622 <212> PRT <213> Candidatus Baumannia cicadellinicola <400> 207 Met Ser Arg Ser Ser Ile Pro Ala Ser Arg Arg Val Ser Arg Ala Lys 1 5 10 15 Ala Gln Ala Phe Met Asp Ser Leu Thr Gly Ser Ser Tyr Phe Pro Asn 20 25 30 Ser Arg Arg Ile Tyr Leu Gln Gly Lys Thr Pro Ser Val His Val Pro 35 40 45 Met Arg Glu Ile Lys Leu His Pro Thr Leu Ile Gly Lys Asn Gly Glu 50 55 60 His Tyr Glu Asp Asn Gln Pro Ile Pro Val Tyr Asp Thr Ser Gly Pro 65 70 75 80 Tyr Gly Asp Pro Thr Ile Ala Ile Asn Val Arg Thr Gly Leu Asn Arg 85 90 95 Leu Arg Glu Ile Trp Ile Leu Ala Arg Gln Asp Ser Glu Pro Ile Ser 100 105 110 Asn Asn Asn Asn Asp Arg Gln Ser Ser Asp Lys Gln Leu Ser Phe Thr 115 120 125 Thr Asn Tyr Asn Pro Arg Arg Ala Ser Tyr Gly Arg Cys Ile Thr Gln 130 135 140 Leu His Tyr Ala Arg Ala Gly Ile Ile Thr Pro Glu Met Glu Phe Ile 145 150 155 160 Ala Leu Arg Glu Asn Met Gly Arg Glu Arg Ile Ser Ser Asn Val Leu 165 170 175 His Gln Gln His Leu Gly Ser Asn Phe Gly Ala Lys Lys Ala Asp His 180 185 190 Ile Thr Ala Glu Phe Val Arg Gln Glu Val Ala Ala Gly Arg Ala Ile 195 200 205 Ile Pro Ser Asn Ile Asn His Pro Glu Ser Glu Pro Met Ile Ile Gly 210 215 220 Arg Asn Phe Leu Val Lys Val Asn Ala Asn Ile Gly Asn Ser Ala Val 225 230 235 240 Thr Ser Ser Ile Glu Glu Glu Val Glu Lys Leu Val Trp Ala Thr Arg 245 250 255 Trp Gly Ala Asp Thr Val Met Asp Leu Ser Thr Gly Ser Tyr Ile His 260 265 270 Glu Thr Arg Glu Trp Ile Leu Arg Asn Ser Pro Val Pro Ile Gly Thr 275 280 285 Val Pro Ile Tyr Gln Ala Leu Glu Lys Val Asn Gly Val Ile Glu Asn 290 295 300 Leu Asn Trp Asp Ile Phe Tyr Glu Thr Leu Leu Glu Gln Ala Asn Gln 305 310 315 320 Gly Val Asp Tyr Phe Thr Ile His Ala Gly Val Leu Lys Arg Tyr Val 325 330 335 Leu Leu Thr Ala Ser Arg Leu Thr Gly Ile Val Ser Arg Gly Gly Ser 340 345 350 Ile Met Ala Gln Trp Ser Leu Val His Asn Gln Glu Asn Phe Leu Tyr 355 360 365 Glu His Phe Ser Glu Ile Cys Lys Leu Cys Ala Ala Tyr Asp Ile Ala 370 375 380 Leu Ser Leu Gly Asp Gly Leu Arg Pro Gly Ser Val Gln Asp Ala Asn 385 390 395 400 Asp Glu Ala Gln Phe Ser Glu Leu His Thr Leu Gly Glu Leu Thr Lys 405 410 415 Ile Ala Trp Glu Tyr Asp Val Gln Val Met Ile Glu Gly Pro Gly His 420 425 430 Ile Pro Leu His Met Ile Glu Arg Asn Met Thr Asp Gln Leu Lys Tyr 435 440 445 Cys His Glu Ala Pro Phe Tyr Thr Leu Gly Pro Leu Thr Thr Asp Ile 450 455 460 Ala Pro Gly Tyr Asp His Phe Thr Ser Gly Ile Gly Ala Ala Leu Ile 465 470 475 480 Gly Trp Phe Gly Cys Ala Met Leu Cys Tyr Val Thr Pro Lys Glu His 485 490 495 Leu Gly Leu Pro Asn Lys Glu Asp Val Lys Gln Gly Leu Ile Ala Tyr 500 505 510 Lys Ile Ala Ala His Ala Ala Asp Leu Ala Lys Gly His Pro Gly Ala 515 520 525 Gln Ile Arg Asp Asn Ala Met Ser Lys Ala Arg Phe Glu Phe Arg Trp 530 535 540 Glu Asp Gln Phe Asn Leu Ala Leu Asp Pro Phe Thr Ala Arg Met Tyr 545 550 555 560 His Asp Glu Thr Ile Pro Gln Thr Ala Gly Lys Leu Ala Asn Phe Cys 565 570 575 Ser Met Cys Gly Pro Lys Phe Cys Ser Met Lys Leu Ser Lys Lys Ile 580 585 590 Arg Asn Tyr Thr Asn Met Lys Asn Ile Lys Thr Ile Ser Asn Ser Phe 595 600 605 Met Asn Lys Leu Asp Asn Ser Gly Ile Lys Asn Ala Asp Arg 610 615 620 <210> 208 <211> 636 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(636) <223> ThiE gene from E. coli encoding thiamine phosphate synthase <400> 208 atg tat cag cct gat ttt cct cct gta cct ttt cgt tca gga ctg tac 48 Met Tyr Gln Pro Asp Phe Pro Pro Val Pro Phe Arg Ser Gly Leu Tyr 1 5 10 15 ccg gtg gtg gac agc gta cag tgg atc gaa cgt ctg ttg gat gca ggc 96 Pro Val Val Asp Ser Val Gln Trp Ile Glu Arg Leu Leu Asp Ala Gly 20 25 30 gta cgt act ctc cag cta cgc atc aaa gat cgg cgc gat gaa gag gtg 144 Val Arg Thr Leu Gln Leu Arg Ile Lys Asp Arg Arg Asp Glu Glu Val 35 40 45 gaa gcc gat gtc gtg gcg gca att gcg ctg ggc cgc cgc tat aac gcg 192 Glu Ala Asp Val Val Ala Ala Ile Ala Leu Gly Arg Arg Tyr Asn Ala 50 55 60 cga ttg ttt atc aac gat tac tgg cgg ctg gcg atc aag cat cag gcg 240 Arg Leu Phe Ile Asn Asp Tyr Trp Arg Leu Ala Ile Lys His Gln Ala 65 70 75 80 tat ggc gtc cat ttg ggg cag gaa gat ttg caa gcc acc gat ctc aat 288 Tyr Gly Val His Leu Gly Gln Glu Asp Leu Gln Ala Thr Asp Leu Asn 85 90 95 gcc atc cgc gcg gca ggc ctg cgg ctg ggc gtt tcg aca cat gac gat 336 Ala Ile Arg Ala Ala Gly Leu Arg Leu Gly Val Ser Thr His Asp Asp 100 105 110 atg gaa atc gac gtc gcg ctg gca gca cgc ccc tct tat atc gcg ctg 384 Met Glu Ile Asp Val Ala Leu Ala Ala Arg Pro Ser Tyr Ile Ala Leu 115 120 125 gga cat gtg ttc ccg acg caa acc aaa cag atg cct tct gca ccg cag 432 Gly His Val Phe Pro Thr Gln Thr Lys Gln Met Pro Ser Ala Pro Gln 130 135 140 ggg ctg gaa cag ctg gca cgg cat gtt gag cga ctg gcg gat tat ccc 480 Gly Leu Glu Gln Leu Ala Arg His Val Glu Arg Leu Ala Asp Tyr Pro 145 150 155 160 acc gtg gcg att ggc ggt atc agt ctg gca cgc gcg cct gcg gtg ata 528 Thr Val Ala Ile Gly Gly Ile Ser Leu Ala Arg Ala Pro Ala Val Ile 165 170 175 gca acg ggt gtc ggc agt atc gcc gtc gtc agc gcc att act caa gcc 576 Ala Thr Gly Val Gly Ser Ile Ala Val Val Ser Ala Ile Thr Gln Ala 180 185 190 gca gac tgg cgt ttg gca acg gca cag ttg ctg gaa att gca gga gtt 624 Ala Asp Trp Arg Leu Ala Thr Ala Gln Leu Leu Glu Ile Ala Gly Val 195 200 205 ggc gat gaa tga 636 Gly Asp Glu 210 <210> 209 <211> 211 <212> PRT <213> Escherichia coli <400> 209 Met Tyr Gln Pro Asp Phe Pro Pro Val Pro Phe Arg Ser Gly Leu Tyr 1 5 10 15 Pro Val Val Asp Ser Val Gln Trp Ile Glu Arg Leu Leu Asp Ala Gly 20 25 30 Val Arg Thr Leu Gln Leu Arg Ile Lys Asp Arg Arg Asp Glu Glu Val 35 40 45 Glu Ala Asp Val Val Ala Ala Ile Ala Leu Gly Arg Arg Tyr Asn Ala 50 55 60 Arg Leu Phe Ile Asn Asp Tyr Trp Arg Leu Ala Ile Lys His Gln Ala 65 70 75 80 Tyr Gly Val His Leu Gly Gln Glu Asp Leu Gln Ala Thr Asp Leu Asn 85 90 95 Ala Ile Arg Ala Ala Gly Leu Arg Leu Gly Val Ser Thr His Asp Asp 100 105 110 Met Glu Ile Asp Val Ala Leu Ala Ala Arg Pro Ser Tyr Ile Ala Leu 115 120 125 Gly His Val Phe Pro Thr Gln Thr Lys Gln Met Pro Ser Ala Pro Gln 130 135 140 Gly Leu Glu Gln Leu Ala Arg His Val Glu Arg Leu Ala Asp Tyr Pro 145 150 155 160 Thr Val Ala Ile Gly Gly Ile Ser Leu Ala Arg Ala Pro Ala Val Ile 165 170 175 Ala Thr Gly Val Gly Ser Ile Ala Val Val Ser Ala Ile Thr Gln Ala 180 185 190 Ala Asp Trp Arg Leu Ala Thr Ala Gln Leu Leu Glu Ile Ala Gly Val 195 200 205 Gly Asp Glu 210 <210> 210 <211> 756 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(756) <223> ThiF gene from E.coli encoding a ThiS adenylyltransferase <400> 210 atg aat gac cgt gac ttt atg cgt tat agc cgc caa atc ctg ctc gac 48 Met Asn Asp Arg Asp Phe Met Arg Tyr Ser Arg Gln Ile Leu Leu Asp 1 5 10 15 gat atc gct ctg gac ggg cag caa aaa ctg ctc gac agc cag gtg ctg 96 Asp Ile Ala Leu Asp Gly Gln Gln Lys Leu Leu Asp Ser Gln Val Leu 20 25 30 att atc ggt ctg ggc ggg ctg ggt aca cct gct gcg ctg tac ctg gcg 144 Ile Ile Gly Leu Gly Gly Leu Gly Thr Pro Ala Ala Leu Tyr Leu Ala 35 40 45 ggc gct ggc gtc ggg acg ctg gta ctg gca gat gac gac gat gtg cat 192 Gly Ala Gly Val Gly Thr Leu Val Leu Ala Asp Asp Asp Asp Val His 50 55 60 tta agc aat ctg caa cga caa atc ctc ttt acc act gaa gat atc gat 240 Leu Ser Asn Leu Gln Arg Gln Ile Leu Phe Thr Thr Glu Asp Ile Asp 65 70 75 80 cgc ccg aaa tcg cag gtc agc caa cag cga ctg aca cag ttg aat ccc 288 Arg Pro Lys Ser Gln Val Ser Gln Gln Arg Leu Thr Gln Leu Asn Pro 85 90 95 gac att caa ctg aca gca tta caa caa cgg tta acg ggt gag gcg tta 336 Asp Ile Gln Leu Thr Ala Leu Gln Gln Arg Leu Thr Gly Glu Ala Leu 100 105 110 aaa gat gcg gtt gca cgg gcc gat gtg gtg ctc gac tgt acc gac aat 384 Lys Asp Ala Val Ala Arg Ala Asp Val Val Leu Asp Cys Thr Asp Asn 115 120 125 atg gcg act cgc cag gag att aat gcc gcc tgc gtg gca ctc aac acg 432 Met Ala Thr Arg Gln Glu Ile Asn Ala Ala Cys Val Ala Leu Asn Thr 130 135 140 ccg ctt atc acc gcc agc gcg gtc gga ttt ggc ggt cag ttg atg gta 480 Pro Leu Ile Thr Ala Ser Ala Val Gly Phe Gly Gly Gln Leu Met Val 145 150 155 160 ctg acg ccg ccc tgg gag cag ggg tgt tac cgc tgc ctg tgg cca gat 528 Leu Thr Pro Pro Trp Glu Gln Gly Cys Tyr Arg Cys Leu Trp Pro Asp 165 170 175 aac cag gag cca gaa cgc aac tgc cgc acg gcg ggc gtg gtt ggc ccg 576 Asn Gln Glu Pro Glu Arg Asn Cys Arg Thr Ala Gly Val Val Gly Pro 180 185 190 gtg gtc ggg gtt atg ggc act ttg cag gca ctg gaa gcc att aag tta 624 Val Val Gly Val Met Gly Thr Leu Gln Ala Leu Glu Ala Ile Lys Leu 195 200 205 tta agc ggt ata gag aca cct gcg gga gaa ctc cga ctg ttc gac ggt 672 Leu Ser Gly Ile Glu Thr Pro Ala Gly Glu Leu Arg Leu Phe Asp Gly 210 215 220 aaa tcg agc cag tgg cgc agc ctg gcg ttg cgc cgc gcc agt ggt tgc 720 Lys Ser Ser Gln Trp Arg Ser Leu Ala Leu Arg Arg Ala Ser Gly Cys 225 230 235 240 ccg gta tgc gga gga agc aat gca gat cct gtt taa 756 Pro Val Cys Gly Gly Ser Asn Ala Asp Pro Val 245 250 <210> 211 <211> 251 <212> PRT <213> Escherichia coli <400> 211 Met Asn Asp Arg Asp Phe Met Arg Tyr Ser Arg Gln Ile Leu Leu Asp 1 5 10 15 Asp Ile Ala Leu Asp Gly Gln Gln Lys Leu Leu Asp Ser Gln Val Leu 20 25 30 Ile Ile Gly Leu Gly Gly Leu Gly Thr Pro Ala Ala Leu Tyr Leu Ala 35 40 45 Gly Ala Gly Val Gly Thr Leu Val Leu Ala Asp Asp Asp Asp Val His 50 55 60 Leu Ser Asn Leu Gln Arg Gln Ile Leu Phe Thr Thr Glu Asp Ile Asp 65 70 75 80 Arg Pro Lys Ser Gln Val Ser Gln Gln Arg Leu Thr Gln Leu Asn Pro 85 90 95 Asp Ile Gln Leu Thr Ala Leu Gln Gln Arg Leu Thr Gly Glu Ala Leu 100 105 110 Lys Asp Ala Val Ala Arg Ala Asp Val Val Leu Asp Cys Thr Asp Asn 115 120 125 Met Ala Thr Arg Gln Glu Ile Asn Ala Ala Cys Val Ala Leu Asn Thr 130 135 140 Pro Leu Ile Thr Ala Ser Ala Val Gly Phe Gly Gly Gln Leu Met Val 145 150 155 160 Leu Thr Pro Pro Trp Glu Gln Gly Cys Tyr Arg Cys Leu Trp Pro Asp 165 170 175 Asn Gln Glu Pro Glu Arg Asn Cys Arg Thr Ala Gly Val Val Gly Pro 180 185 190 Val Val Gly Val Met Gly Thr Leu Gln Ala Leu Glu Ala Ile Lys Leu 195 200 205 Leu Ser Gly Ile Glu Thr Pro Ala Gly Glu Leu Arg Leu Phe Asp Gly 210 215 220 Lys Ser Ser Gln Trp Arg Ser Leu Ala Leu Arg Arg Ala Ser Gly Cys 225 230 235 240 Pro Val Cys Gly Gly Ser Asn Ala Asp Pro Val 245 250 <210> 212 <211> 201 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(201) <223> ThiS gene from E. coli encoding Sulfur-carrier protein <400> 212 atg cag atc ctg ttt aac gat caa gcg atg cag tgc gcc gcc ggg caa 48 Met Gln Ile Leu Phe Asn Asp Gln Ala Met Gln Cys Ala Ala Gly Gln 1 5 10 15 act gtt cac gaa cta ctg gag caa ctc gac caa cga caa gcg ggc gcg 96 Thr Val His Glu Leu Leu Glu Gln Leu Asp Gln Arg Gln Ala Gly Ala 20 25 30 gct ctg gcg att aat cag caa atc gtc ccg cgt gag cag tgg gcg caa 144 Ala Leu Ala Ile Asn Gln Gln Ile Val Pro Arg Glu Gln Trp Ala Gln 35 40 45 cat atc gtg cag gat ggc gac cag atc ctg ctt ttt cag gtt att gca 192 His Ile Val Gln Asp Gly Asp Gln Ile Leu Leu Phe Gln Val Ile Ala 50 55 60 ggg ggt tga 201 Gly Gly 65 <210> 213 <211> 66 <212> PRT <213> Escherichia coli <400> 213 Met Gln Ile Leu Phe Asn Asp Gln Ala Met Gln Cys Ala Ala Gly Gln 1 5 10 15 Thr Val His Glu Leu Leu Glu Gln Leu Asp Gln Arg Gln Ala Gly Ala 20 25 30 Ala Leu Ala Ile Asn Gln Gln Ile Val Pro Arg Glu Gln Trp Ala Gln 35 40 45 His Ile Val Gln Asp Gly Asp Gln Ile Leu Leu Phe Gln Val Ile Ala 50 55 60 Gly Gly 65 <210> 214 <211> 771 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(771) <223> ThiG gene from E. coli encoding Thiazole synthase <400> 214 atg tta cgt att gcg gac aaa acg ttt gat tca cat ctg ttt acc ggc 48 Met Leu Arg Ile Ala Asp Lys Thr Phe Asp Ser His Leu Phe Thr Gly 1 5 10 15 aca ggg aaa ttc gct tct tca caa ctg atg gtg gag gcg atc cgc gct 96 Thr Gly Lys Phe Ala Ser Ser Gln Leu Met Val Glu Ala Ile Arg Ala 20 25 30 tcc ggc agc cag ctg gtg aca ctg gcg atg aaa cgt gtc gac ttg cgc 144 Ser Gly Ser Gln Leu Val Thr Leu Ala Met Lys Arg Val Asp Leu Arg 35 40 45 cag cac aac gac gct atc ctc gaa ccg ctt atc gcg gcg ggt gtg acc 192 Gln His Asn Asp Ala Ile Leu Glu Pro Leu Ile Ala Ala Gly Val Thr 50 55 60 ctg ctg cca aat aca tcc ggg gcg aaa aca gcg gaa gaa gcc att ttc 240 Leu Leu Pro Asn Thr Ser Gly Ala Lys Thr Ala Glu Glu Ala Ile Phe 65 70 75 80 gcc gcc cat ctg gct cgt gaa gcg tta ggc aca aac tgg tta aaa tta 288 Ala Ala His Leu Ala Arg Glu Ala Leu Gly Thr Asn Trp Leu Lys Leu 85 90 95 gag att cac cct gac gcc cgc tgg ctg ttg ccc gat ccc atc gaa acc 336 Glu Ile His Pro Asp Ala Arg Trp Leu Leu Pro Asp Pro Ile Glu Thr 100 105 110 ctg aaa gcc gcc gaa acg ctg gta caa cag gga ttt gtc gtg ctg cct 384 Leu Lys Ala Ala Glu Thr Leu Val Gln Gln Gly Phe Val Val Leu Pro 115 120 125 tac tgc ggg gcc gat ccg gta ttg tgt aaa cgt ctg gaa gaa gtc ggc 432 Tyr Cys Gly Ala Asp Pro Val Leu Cys Lys Arg Leu Glu Glu Val Gly 130 135 140 tgt gca gcg gtg atg ccg ctc ggc gcg ccg att ggc tcg aat cag gga 480 Cys Ala Ala Val Met Pro Leu Gly Ala Pro Ile Gly Ser Asn Gln Gly 145 150 155 160 ctg gaa acc cgc gcc atg ctg gag att att atc cag cag gcc aca gtg 528 Leu Glu Thr Arg Ala Met Leu Glu Ile Ile Ile Gln Gln Ala Thr Val 165 170 175 ccg gtg gtt gtc gat gct ggc atc ggc gtt ccc agc cat gcc gcg cag 576 Pro Val Val Val Asp Ala Gly Ile Gly Val Pro Ser His Ala Ala Gln 180 185 190 gcg ctg gaa atg ggg gcc gac gcg gtg tta gtg aat acg gcg att gcc 624 Ala Leu Glu Met Gly Ala Asp Ala Val Leu Val Asn Thr Ala Ile Ala 195 200 205 gtc gcg gac gat ccc gtc aac atg gcg aag gca ttt cgt ctg gcg gta 672 Val Ala Asp Asp Pro Val Asn Met Ala Lys Ala Phe Arg Leu Ala Val 210 215 220 gaa gca ggc cta ctg gca cgt cag tcc gga ccg ggc agc cgc agt tat 720 Glu Ala Gly Leu Leu Ala Arg Gln Ser Gly Pro Gly Ser Arg Ser Tyr 225 230 235 240 ttt gct cat gcc acc agc ccg ctg acc gga ttt ctg gag gca tcg gca 768 Phe Ala His Ala Thr Ser Pro Leu Thr Gly Phe Leu Glu Ala Ser Ala 245 250 255 tga 771 <210> 215 <211> 256 <212> PRT <213> Escherichia coli <400> 215 Met Leu Arg Ile Ala Asp Lys Thr Phe Asp Ser His Leu Phe Thr Gly 1 5 10 15 Thr Gly Lys Phe Ala Ser Ser Gln Leu Met Val Glu Ala Ile Arg Ala 20 25 30 Ser Gly Ser Gln Leu Val Thr Leu Ala Met Lys Arg Val Asp Leu Arg 35 40 45 Gln His Asn Asp Ala Ile Leu Glu Pro Leu Ile Ala Ala Gly Val Thr 50 55 60 Leu Leu Pro Asn Thr Ser Gly Ala Lys Thr Ala Glu Glu Ala Ile Phe 65 70 75 80 Ala Ala His Leu Ala Arg Glu Ala Leu Gly Thr Asn Trp Leu Lys Leu 85 90 95 Glu Ile His Pro Asp Ala Arg Trp Leu Leu Pro Asp Pro Ile Glu Thr 100 105 110 Leu Lys Ala Ala Glu Thr Leu Val Gln Gln Gly Phe Val Val Leu Pro 115 120 125 Tyr Cys Gly Ala Asp Pro Val Leu Cys Lys Arg Leu Glu Glu Val Gly 130 135 140 Cys Ala Ala Val Met Pro Leu Gly Ala Pro Ile Gly Ser Asn Gln Gly 145 150 155 160 Leu Glu Thr Arg Ala Met Leu Glu Ile Ile Ile Gln Gln Ala Thr Val 165 170 175 Pro Val Val Val Asp Ala Gly Ile Gly Val Pro Ser His Ala Ala Gln 180 185 190 Ala Leu Glu Met Gly Ala Asp Ala Val Leu Val Asn Thr Ala Ile Ala 195 200 205 Val Ala Asp Asp Pro Val Asn Met Ala Lys Ala Phe Arg Leu Ala Val 210 215 220 Glu Ala Gly Leu Leu Ala Arg Gln Ser Gly Pro Gly Ser Arg Ser Tyr 225 230 235 240 Phe Ala His Ala Thr Ser Pro Leu Thr Gly Phe Leu Glu Ala Ser Ala 245 250 255 <210> 216 <211> 1134 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(1134) <223> ThiH gene from E. coli encoding 2-iminoacetate synthase <400> 216 atg aaa acc ttc agc gat cgc tgg cga caa ctg gac tgg gac gac atc 48 Met Lys Thr Phe Ser Asp Arg Trp Arg Gln Leu Asp Trp Asp Asp Ile 1 5 10 15 cgc ctg cgt atc aac ggc aaa acg gct gct gac gta gag cgg gcg cta 96 Arg Leu Arg Ile Asn Gly Lys Thr Ala Ala Asp Val Glu Arg Ala Leu 20 25 30 aat gcc tcg caa ctc acc cgc gac gac atg atg gcg ctg tta tcg cct 144 Asn Ala Ser Gln Leu Thr Arg Asp Asp Met Met Ala Leu Leu Ser Pro 35 40 45 gcc gcc agt ggc tat ctg gaa caa ctg gcc caa cgg gcg cag cgt ctg 192 Ala Ala Ser Gly Tyr Leu Glu Gln Leu Ala Gln Arg Ala Gln Arg Leu 50 55 60 acc cgt cag cga ttt ggc aac aca gtt agt ttc tac gtc ccg ctt tat 240 Thr Arg Gln Arg Phe Gly Asn Thr Val Ser Phe Tyr Val Pro Leu Tyr 65 70 75 80 ctt tcc aat ctt tgc gct aac gac tgc acg tac tgt gga ttt tcc atg 288 Leu Ser Asn Leu Cys Ala Asn Asp Cys Thr Tyr Cys Gly Phe Ser Met 85 90 95 agt aat cgc atc aag cgc aaa acg ctg gat gaa gcg gat att gcc agg 336 Ser Asn Arg Ile Lys Arg Lys Thr Leu Asp Glu Ala Asp Ile Ala Arg 100 105 110 gaa agt gcc gct ata cgg gag atg ggc ttt gaa cat ctg ctg tta gtc 384 Glu Ser Ala Ala Ile Arg Glu Met Gly Phe Glu His Leu Leu Leu Val 115 120 125 act ggt gaa cat cag gcg aaa gtg ggg atg gat tac ttt cgt cgt cat 432 Thr Gly Glu His Gln Ala Lys Val Gly Met Asp Tyr Phe Arg Arg His 130 135 140 ctc cct gcc ctt cgt gaa cag ttc tct tca cta cag atg gaa gtg caa 480 Leu Pro Ala Leu Arg Glu Gln Phe Ser Ser Leu Gln Met Glu Val Gln 145 150 155 160 ccg ctg gcg gag acg gaa tac gcc gag tta aag caa ctt ggt ctg gat 528 Pro Leu Ala Glu Thr Glu Tyr Ala Glu Leu Lys Gln Leu Gly Leu Asp 165 170 175 ggc gtg atg gtt tat cag gag aca tat cac gag gcg act tat gcc cgc 576 Gly Val Met Val Tyr Gln Glu Thr Tyr His Glu Ala Thr Tyr Ala Arg 180 185 190 cat cat ctg aaa ggc aaa aaa cag gac ttc ttc tgg cgg ctg gaa acg 624 His His Leu Lys Gly Lys Lys Gln Asp Phe Phe Trp Arg Leu Glu Thr 195 200 205 ccg gat cgg ctg ggg cgt gcg ggg att gat aag ata ggc ctc ggc gcg 672 Pro Asp Arg Leu Gly Arg Ala Gly Ile Asp Lys Ile Gly Leu Gly Ala 210 215 220 cta att ggc ctt tcc gac aac tgg cgc gtt gac agc tat atg gtt gcc 720 Leu Ile Gly Leu Ser Asp Asn Trp Arg Val Asp Ser Tyr Met Val Ala 225 230 235 240 gaa cat ttg cta tgg ctg caa cag cat tac tgg caa agc cgt tac tct 768 Glu His Leu Leu Trp Leu Gln Gln His Tyr Trp Gln Ser Arg Tyr Ser 245 250 255 gtc tcc ttt ccg cgc ctg cgc ccg tgt act ggc ggc att gag cct gcg 816 Val Ser Phe Pro Arg Leu Arg Pro Cys Thr Gly Gly Ile Glu Pro Ala 260 265 270 tcg att atg gat gaa cgc cag tta gtg caa acc atc tgc gcc ttc cga 864 Ser Ile Met Asp Glu Arg Gln Leu Val Gln Thr Ile Cys Ala Phe Arg 275 280 285 ctg ctt gca ccg gag att gaa ctg tca ctc tcc acg cgg gaa tca ccg 912 Leu Leu Ala Pro Glu Ile Glu Leu Ser Leu Ser Thr Arg Glu Ser Pro 290 295 300 tgg ttt cgc gat cgc gtt att ccg ctg gcg atc aat aac gtc agc gcc 960 Trp Phe Arg Asp Arg Val Ile Pro Leu Ala Ile Asn Asn Val Ser Ala 305 310 315 320 ttc tcg aaa acg cag cca ggt ggc tat gcc gat aat cac ccc gag ttg 1008 Phe Ser Lys Thr Gln Pro Gly Gly Tyr Ala Asp Asn His Pro Glu Leu 325 330 335 gaa cag ttc tca ccg cac gac gat cgc aga ccg gaa gcg gtt gct gcc 1056 Glu Gln Phe Ser Pro His Asp Asp Arg Arg Pro Glu Ala Val Ala Ala 340 345 350 gcg tta acc gct cag ggt ttg cag ccg gta tgg aaa gac tgg gac agc 1104 Ala Leu Thr Ala Gln Gly Leu Gln Pro Val Trp Lys Asp Trp Asp Ser 355 360 365 tat ctg gga cgc gcc tcg caa aga cta tga 1134 Tyr Leu Gly Arg Ala Ser Gln Arg Leu 370 375 <210> 217 <211> 377 <212> PRT <213> Escherichia coli <400> 217 Met Lys Thr Phe Ser Asp Arg Trp Arg Gln Leu Asp Trp Asp Asp Ile 1 5 10 15 Arg Leu Arg Ile Asn Gly Lys Thr Ala Ala Asp Val Glu Arg Ala Leu 20 25 30 Asn Ala Ser Gln Leu Thr Arg Asp Asp Met Met Ala Leu Leu Ser Pro 35 40 45 Ala Ala Ser Gly Tyr Leu Glu Gln Leu Ala Gln Arg Ala Gln Arg Leu 50 55 60 Thr Arg Gln Arg Phe Gly Asn Thr Val Ser Phe Tyr Val Pro Leu Tyr 65 70 75 80 Leu Ser Asn Leu Cys Ala Asn Asp Cys Thr Tyr Cys Gly Phe Ser Met 85 90 95 Ser Asn Arg Ile Lys Arg Lys Thr Leu Asp Glu Ala Asp Ile Ala Arg 100 105 110 Glu Ser Ala Ala Ile Arg Glu Met Gly Phe Glu His Leu Leu Leu Val 115 120 125 Thr Gly Glu His Gln Ala Lys Val Gly Met Asp Tyr Phe Arg Arg His 130 135 140 Leu Pro Ala Leu Arg Glu Gln Phe Ser Ser Leu Gln Met Glu Val Gln 145 150 155 160 Pro Leu Ala Glu Thr Glu Tyr Ala Glu Leu Lys Gln Leu Gly Leu Asp 165 170 175 Gly Val Met Val Tyr Gln Glu Thr Tyr His Glu Ala Thr Tyr Ala Arg 180 185 190 His His Leu Lys Gly Lys Lys Gln Asp Phe Phe Trp Arg Leu Glu Thr 195 200 205 Pro Asp Arg Leu Gly Arg Ala Gly Ile Asp Lys Ile Gly Leu Gly Ala 210 215 220 Leu Ile Gly Leu Ser Asp Asn Trp Arg Val Asp Ser Tyr Met Val Ala 225 230 235 240 Glu His Leu Leu Trp Leu Gln Gln His Tyr Trp Gln Ser Arg Tyr Ser 245 250 255 Val Ser Phe Pro Arg Leu Arg Pro Cys Thr Gly Gly Ile Glu Pro Ala 260 265 270 Ser Ile Met Asp Glu Arg Gln Leu Val Gln Thr Ile Cys Ala Phe Arg 275 280 285 Leu Leu Ala Pro Glu Ile Glu Leu Ser Leu Ser Thr Arg Glu Ser Pro 290 295 300 Trp Phe Arg Asp Arg Val Ile Pro Leu Ala Ile Asn Asn Val Ser Ala 305 310 315 320 Phe Ser Lys Thr Gln Pro Gly Gly Tyr Ala Asp Asn His Pro Glu Leu 325 330 335 Glu Gln Phe Ser Pro His Asp Asp Arg Arg Pro Glu Ala Val Ala Ala 340 345 350 Ala Leu Thr Ala Gln Gly Leu Gln Pro Val Trp Lys Asp Trp Asp Ser 355 360 365 Tyr Leu Gly Arg Ala Ser Gln Arg Leu 370 375 <210> 218 <211> 1110 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(1110) <223> ThiO gene from E. coli encoding Glycine oxidase <400> 218 atg aaa agg cat tat gaa gca gtg gtg att gga ggc gga att atc ggt 48 Met Lys Arg His Tyr Glu Ala Val Val Ile Gly Gly Gly Ile Ile Gly 1 5 10 15 tcc gca att gct tat tat ttg gca aag gaa aac aaa aac acc gca ttg 96 Ser Ala Ile Ala Tyr Tyr Leu Ala Lys Glu Asn Lys Asn Thr Ala Leu 20 25 30 ttt gaa agc gga aca atg ggc ggc aga acg aca agt gcc gct gcc gga 144 Phe Glu Ser Gly Thr Met Gly Gly Arg Thr Thr Ser Ala Ala Ala Gly 35 40 45 atg ctg ggc gcc cat gcc gaa tgc gag gaa cgt gac gcg ttt ttt gat 192 Met Leu Gly Ala His Ala Glu Cys Glu Glu Arg Asp Ala Phe Phe Asp 50 55 60 ttc gct atg cac agt cag cgt ctg tac aaa ggt ctt gga gaa gag ctt 240 Phe Ala Met His Ser Gln Arg Leu Tyr Lys Gly Leu Gly Glu Glu Leu 65 70 75 80 tat gca tta tcc ggt gtg gat atc agg cag cat aac ggc ggt atg ttt 288 Tyr Ala Leu Ser Gly Val Asp Ile Arg Gln His Asn Gly Gly Met Phe 85 90 95 aag ctt gca ttt tct gaa gaa gat gtg ctg cag ctg aga cag atg gac 336 Lys Leu Ala Phe Ser Glu Glu Asp Val Leu Gln Leu Arg Gln Met Asp 100 105 110 gat ttg gac tct gtc agc tgg tat tca aaa gaa gag gtg tta gaa aaa 384 Asp Leu Asp Ser Val Ser Trp Tyr Ser Lys Glu Glu Val Leu Glu Lys 115 120 125 gag ccg tat gcg tct ggt gac atc ttt ggt gca tct ttt att cag gat 432 Glu Pro Tyr Ala Ser Gly Asp Ile Phe Gly Ala Ser Phe Ile Gln Asp 130 135 140 gat gtg cat gtg gag cct tat ttt gtt tgc aag gca tat gtg aaa gca 480 Asp Val His Val Glu Pro Tyr Phe Val Cys Lys Ala Tyr Val Lys Ala 145 150 155 160 gca aaa atg ctt ggg gcg gag att ttt gag cat acg ccc gtc ctg cat 528 Ala Lys Met Leu Gly Ala Glu Ile Phe Glu His Thr Pro Val Leu His 165 170 175 gtc gaa cgt gac ggt gaa gcc ctg ttc atc aag acc cct agc gga gac 576 Val Glu Arg Asp Gly Glu Ala Leu Phe Ile Lys Thr Pro Ser Gly Asp 180 185 190 gta tgg gct aat cat gtt gtc gtt gcc agc ggg gtg tgg agc gga atg 624 Val Trp Ala Asn His Val Val Val Ala Ser Gly Val Trp Ser Gly Met 195 200 205 ttt ttt aaa cag ctt gga ctg aac aat gct ttt ctc cct gta aaa ggg 672 Phe Phe Lys Gln Leu Gly Leu Asn Asn Ala Phe Leu Pro Val Lys Gly 210 215 220 gag tgc ctg tcc gtt tgg aat gat gat atc ccg ctg aca aaa acg ctt 720 Glu Cys Leu Ser Val Trp Asn Asp Asp Ile Pro Leu Thr Lys Thr Leu 225 230 235 240 tac cat gat cac tgc tat atc gta ccg aga aaa agc gga aga ctg gtt 768 Tyr His Asp His Cys Tyr Ile Val Pro Arg Lys Ser Gly Arg Leu Val 245 250 255 gtc ggc gcg aca atg aag ccg ggg gac tgg agt gaa aca ccg gat ctt 816 Val Gly Ala Thr Met Lys Pro Gly Asp Trp Ser Glu Thr Pro Asp Leu 260 265 270 ggc gga ttg gaa tct gtt atg aaa aaa gca aaa acg atg ctg ccg gct 864 Gly Gly Leu Glu Ser Val Met Lys Lys Ala Lys Thr Met Leu Pro Ala 275 280 285 ata cag aat atg aag gtg gat cgt ttt tgg gcg gga ctc cgt ccg gga 912 Ile Gln Asn Met Lys Val Asp Arg Phe Trp Ala Gly Leu Arg Pro Gly 290 295 300 aca aag gat gga aaa ccg tac atc ggc aga cat cct gag gac agc cgt 960 Thr Lys Asp Gly Lys Pro Tyr Ile Gly Arg His Pro Glu Asp Ser Arg 305 310 315 320 att tta ttt gcg gct ggc cat ttc aga aac ggg atc ctg ctt gct ccc 1008 Ile Leu Phe Ala Ala Gly His Phe Arg Asn Gly Ile Leu Leu Ala Pro 325 330 335 gca acg ggc gct ttg atc agt gat ctc atc atg aat aaa gag gtc aac 1056 Ala Thr Gly Ala Leu Ile Ser Asp Leu Ile Met Asn Lys Glu Val Asn 340 345 350 caa gac tgg ctg cac gca ttc cga att gat cgc aag gag gcg gtt cag 1104 Gln Asp Trp Leu His Ala Phe Arg Ile Asp Arg Lys Glu Ala Val Gln 355 360 365 ata tga 1110 Ile <210> 219 <211> 369 <212> PRT <213> Escherichia coli <400> 219 Met Lys Arg His Tyr Glu Ala Val Val Ile Gly Gly Gly Ile Ile Gly 1 5 10 15 Ser Ala Ile Ala Tyr Tyr Leu Ala Lys Glu Asn Lys Asn Thr Ala Leu 20 25 30 Phe Glu Ser Gly Thr Met Gly Gly Arg Thr Thr Ser Ala Ala Ala Gly 35 40 45 Met Leu Gly Ala His Ala Glu Cys Glu Glu Arg Asp Ala Phe Phe Asp 50 55 60 Phe Ala Met His Ser Gln Arg Leu Tyr Lys Gly Leu Gly Glu Glu Leu 65 70 75 80 Tyr Ala Leu Ser Gly Val Asp Ile Arg Gln His Asn Gly Gly Met Phe 85 90 95 Lys Leu Ala Phe Ser Glu Glu Asp Val Leu Gln Leu Arg Gln Met Asp 100 105 110 Asp Leu Asp Ser Val Ser Trp Tyr Ser Lys Glu Glu Val Leu Glu Lys 115 120 125 Glu Pro Tyr Ala Ser Gly Asp Ile Phe Gly Ala Ser Phe Ile Gln Asp 130 135 140 Asp Val His Val Glu Pro Tyr Phe Val Cys Lys Ala Tyr Val Lys Ala 145 150 155 160 Ala Lys Met Leu Gly Ala Glu Ile Phe Glu His Thr Pro Val Leu His 165 170 175 Val Glu Arg Asp Gly Glu Ala Leu Phe Ile Lys Thr Pro Ser Gly Asp 180 185 190 Val Trp Ala Asn His Val Val Val Ala Ser Gly Val Trp Ser Gly Met 195 200 205 Phe Phe Lys Gln Leu Gly Leu Asn Asn Ala Phe Leu Pro Val Lys Gly 210 215 220 Glu Cys Leu Ser Val Trp Asn Asp Asp Ile Pro Leu Thr Lys Thr Leu 225 230 235 240 Tyr His Asp His Cys Tyr Ile Val Pro Arg Lys Ser Gly Arg Leu Val 245 250 255 Val Gly Ala Thr Met Lys Pro Gly Asp Trp Ser Glu Thr Pro Asp Leu 260 265 270 Gly Gly Leu Glu Ser Val Met Lys Lys Ala Lys Thr Met Leu Pro Ala 275 280 285 Ile Gln Asn Met Lys Val Asp Arg Phe Trp Ala Gly Leu Arg Pro Gly 290 295 300 Thr Lys Asp Gly Lys Pro Tyr Ile Gly Arg His Pro Glu Asp Ser Arg 305 310 315 320 Ile Leu Phe Ala Ala Gly His Phe Arg Asn Gly Ile Leu Leu Ala Pro 325 330 335 Ala Thr Gly Ala Leu Ile Ser Asp Leu Ile Met Asn Lys Glu Val Asn 340 345 350 Gln Asp Trp Leu His Ala Phe Arg Ile Asp Arg Lys Glu Ala Val Gln 355 360 365 Ile <210> 220 <211> 1098 <212> DNA <213> Pseudomonas putida <220> <221> CDS <222> (1)..(1098) <223> ThiO gene from Pseudomonas putida encoding a Glycine oxidase <400> 220 atg agc aag caa gta gtg gtg gtc ggt ggc ggg gtc att ggc ctg ctg 48 Met Ser Lys Gln Val Val Val Val Gly Gly Gly Val Ile Gly Leu Leu 1 5 10 15 acg gca ttc aac ctg gcg gcg agc gtc gac cag gtg gtg gta tgc gac 96 Thr Ala Phe Asn Leu Ala Ala Ser Val Asp Gln Val Val Val Cys Asp 20 25 30 cag ggc gaa gta ggg cgc gag tcc tcc tgg gct ggg ggc ggt atc gtc 144 Gln Gly Glu Val Gly Arg Glu Ser Ser Trp Ala Gly Gly Gly Ile Val 35 40 45 tcg ccc ctg tat cct tgg cgc tac agc ccg gca gtg acc gcc ctg gcg 192 Ser Pro Leu Tyr Pro Trp Arg Tyr Ser Pro Ala Val Thr Ala Leu Ala 50 55 60 cat tgg tcg cag gac ttt tac cca cag ttg ggc gag cgc ttg ttc gcc 240 His Trp Ser Gln Asp Phe Tyr Pro Gln Leu Gly Glu Arg Leu Phe Ala 65 70 75 80 agc acg ggc ctg gat cct gag gtg cat acc acc ggg ctt tac tgg ctc 288 Ser Thr Gly Leu Asp Pro Glu Val His Thr Thr Gly Leu Tyr Trp Leu 85 90 95 gac ctg gat gac caa gcc cag gcc ttg gcg tgg gca ggc cgt cag cag 336 Asp Leu Asp Asp Gln Ala Gln Ala Leu Ala Trp Ala Gly Arg Gln Gln 100 105 110 cgt ccg ctg agc gcc gtg gat att tca gcg gtg tac gac gca gtc cct 384 Arg Pro Leu Ser Ala Val Asp Ile Ser Ala Val Tyr Asp Ala Val Pro 115 120 125 gtg ctg ggg cca ggc ttt gag cga gcc ctc tac atg gaa ggc gtg gcc 432 Val Leu Gly Pro Gly Phe Glu Arg Ala Leu Tyr Met Glu Gly Val Ala 130 135 140 aat gtg cgc aac ccg cgc ctg gtc aaa tcg ctg aag gcg gcg ttg ctg 480 Asn Val Arg Asn Pro Arg Leu Val Lys Ser Leu Lys Ala Ala Leu Leu 145 150 155 160 gca ttg ccc aat gtg agc gtg cgc gag cac tgc cag atc acg ggg ttc 528 Ala Leu Pro Asn Val Ser Val Arg Glu His Cys Gln Ile Thr Gly Phe 165 170 175 gtg cag cag ggc gct cgt atc att ggg gtg agc acc gct gaa ggc gag 576 Val Gln Gln Gly Ala Arg Ile Ile Gly Val Ser Thr Ala Glu Gly Glu 180 185 190 ctg gcc gcc gac gaa gtc gta ctg agc gcc ggt gcc tgg agc ggc gaa 624 Leu Ala Ala Asp Glu Val Val Leu Ser Ala Gly Ala Trp Ser Gly Glu 195 200 205 ctg ctg cgc cac ttg ggc ctt gag ctt cca gtc gag ccg gta aaa ggg 672 Leu Leu Arg His Leu Gly Leu Glu Leu Pro Val Glu Pro Val Lys Gly 210 215 220 cag atg atc ctg ttc aaa tgc gct gaa gat ttt ctg cca agc atg gtg 720 Gln Met Ile Leu Phe Lys Cys Ala Glu Asp Phe Leu Pro Ser Met Val 225 230 235 240 ctt gcc aaa ggt cgt tat gca att ccg cgt cgg gat ggt cac att ctg 768 Leu Ala Lys Gly Arg Tyr Ala Ile Pro Arg Arg Asp Gly His Ile Leu 245 250 255 gtg ggc agc acg ctg gag cat gcc ggc tac gac aag aca ccc acc gat 816 Val Gly Ser Thr Leu Glu His Ala Gly Tyr Asp Lys Thr Pro Thr Asp 260 265 270 gag gcg ttg gcc agc ctc aag gca tcg gcg gtg gat ctg ctg ccc ggc 864 Glu Ala Leu Ala Ser Leu Lys Ala Ser Ala Val Asp Leu Leu Pro Gly 275 280 285 ctg gaa ggc gcg cac gtg gtt gcc cac tgg gcc ggg ctg cgg cca ggt 912 Leu Glu Gly Ala His Val Val Ala His Trp Ala Gly Leu Arg Pro Gly 290 295 300 tcg cca gaa ggc gtt ccg ttt atc ggg ccg gta ccc ggc ttc gat ggg 960 Ser Pro Glu Gly Val Pro Phe Ile Gly Pro Val Pro Gly Phe Asp Gly 305 310 315 320 tta tgg ctg aac tgc ggc cat tac cga aac ggg ctg gtg ctg gcg ccc 1008 Leu Trp Leu Asn Cys Gly His Tyr Arg Asn Gly Leu Val Leu Ala Pro 325 330 335 gct tcg tgc caa ctg ctg gcc gat ttg ctc aat ggc gcc gag ccc atc 1056 Ala Ser Cys Gln Leu Leu Ala Asp Leu Leu Asn Gly Ala Glu Pro Ile 340 345 350 atc gac ccg tca ccc tac gcc ccg tct ggg cgc ctt ggc taa 1098 Ile Asp Pro Ser Pro Tyr Ala Pro Ser Gly Arg Leu Gly 355 360 365 <210> 221 <211> 365 <212> PRT <213> Pseudomonas putida <400> 221 Met Ser Lys Gln Val Val Val Val Gly Gly Gly Val Ile Gly Leu Leu 1 5 10 15 Thr Ala Phe Asn Leu Ala Ala Ser Val Asp Gln Val Val Val Cys Asp 20 25 30 Gln Gly Glu Val Gly Arg Glu Ser Ser Trp Ala Gly Gly Gly Ile Val 35 40 45 Ser Pro Leu Tyr Pro Trp Arg Tyr Ser Pro Ala Val Thr Ala Leu Ala 50 55 60 His Trp Ser Gln Asp Phe Tyr Pro Gln Leu Gly Glu Arg Leu Phe Ala 65 70 75 80 Ser Thr Gly Leu Asp Pro Glu Val His Thr Thr Gly Leu Tyr Trp Leu 85 90 95 Asp Leu Asp Asp Gln Ala Gln Ala Leu Ala Trp Ala Gly Arg Gln Gln 100 105 110 Arg Pro Leu Ser Ala Val Asp Ile Ser Ala Val Tyr Asp Ala Val Pro 115 120 125 Val Leu Gly Pro Gly Phe Glu Arg Ala Leu Tyr Met Glu Gly Val Ala 130 135 140 Asn Val Arg Asn Pro Arg Leu Val Lys Ser Leu Lys Ala Ala Leu Leu 145 150 155 160 Ala Leu Pro Asn Val Ser Val Arg Glu His Cys Gln Ile Thr Gly Phe 165 170 175 Val Gln Gln Gly Ala Arg Ile Ile Gly Val Ser Thr Ala Glu Gly Glu 180 185 190 Leu Ala Ala Asp Glu Val Val Leu Ser Ala Gly Ala Trp Ser Gly Glu 195 200 205 Leu Leu Arg His Leu Gly Leu Glu Leu Pro Val Glu Pro Val Lys Gly 210 215 220 Gln Met Ile Leu Phe Lys Cys Ala Glu Asp Phe Leu Pro Ser Met Val 225 230 235 240 Leu Ala Lys Gly Arg Tyr Ala Ile Pro Arg Arg Asp Gly His Ile Leu 245 250 255 Val Gly Ser Thr Leu Glu His Ala Gly Tyr Asp Lys Thr Pro Thr Asp 260 265 270 Glu Ala Leu Ala Ser Leu Lys Ala Ser Ala Val Asp Leu Leu Pro Gly 275 280 285 Leu Glu Gly Ala His Val Val Ala His Trp Ala Gly Leu Arg Pro Gly 290 295 300 Ser Pro Glu Gly Val Pro Phe Ile Gly Pro Val Pro Gly Phe Asp Gly 305 310 315 320 Leu Trp Leu Asn Cys Gly His Tyr Arg Asn Gly Leu Val Leu Ala Pro 325 330 335 Ala Ser Cys Gln Leu Leu Ala Asp Leu Leu Asn Gly Ala Glu Pro Ile 340 345 350 Ile Asp Pro Ser Pro Tyr Ala Pro Ser Gly Arg Leu Gly 355 360 365 <210> 222 <211> 1140 <212> DNA <213> Synechococcus elongatus <220> <221> CDS <222> (1)..(1140) <223> ThiO gene from Synechococcus elongatus encoding a Glycine oxidase <400> 222 atg gcg ttc gag gta gcc gtc ttt ggg ggc ggc gtc att ggc ttg gcg 48 Met Ala Phe Glu Val Ala Val Phe Gly Gly Gly Val Ile Gly Leu Ala 1 5 10 15 atc gcg cta gaa ctg cga tcg cga ggc gcg atg gtg cag gtc tac agt 96 Ile Ala Leu Glu Leu Arg Ser Arg Gly Ala Met Val Gln Val Tyr Ser 20 25 30 caa aac act cag gcg gcg gca ggt cgt gtg gca gca ggg atg ttg gcg 144 Gln Asn Thr Gln Ala Ala Ala Gly Arg Val Ala Ala Gly Met Leu Ala 35 40 45 ccc cag tcg gaa ggc atc gaa gtc ggg ccc atg ctg gat ctg ggg ctg 192 Pro Gln Ser Glu Gly Ile Glu Val Gly Pro Met Leu Asp Leu Gly Leu 50 55 60 cgc agc cga tcg ctc tac gcc cgc tgg acc cag caa ctc gaa caa ctc 240 Arg Ser Arg Ser Leu Tyr Ala Arg Trp Thr Gln Gln Leu Glu Gln Leu 65 70 75 80 agc ggt caa gac agt ggc tac tgg ccc tgc ggc att ttg gtg ccc ctg 288 Ser Gly Gln Asp Ser Gly Tyr Trp Pro Cys Gly Ile Leu Val Pro Leu 85 90 95 agt gag gcc aaa aat cgc gat cgc tat cct cat cca gca gaa tct ccg 336 Ser Glu Ala Lys Asn Arg Asp Arg Tyr Pro His Pro Ala Glu Ser Pro 100 105 110 ggg caa tgg ctc tcg gca gcg gac tta cga gac ttt cag ccc gca cta 384 Gly Gln Trp Leu Ser Ala Ala Asp Leu Arg Asp Phe Gln Pro Ala Leu 115 120 125 tgc tct gac cta atc ggt ggc tgg tgg ttt tcc caa gaa ggg caa gtt 432 Cys Ser Asp Leu Ile Gly Gly Trp Trp Phe Ser Gln Glu Gly Gln Val 130 135 140 gat agt cgc cgt gcc ctg tat cca gcg ctg cga gcc gcc gcg atc gcc 480 Asp Ser Arg Arg Ala Leu Tyr Pro Ala Leu Arg Ala Ala Ala Ile Ala 145 150 155 160 agt ggc gtc acg atc cat gaa agc gtg gcg ctg cgg gag tta tct gta 528 Ser Gly Val Thr Ile His Glu Ser Val Ala Leu Arg Glu Leu Ser Val 165 170 175 aca ggc gat cgc ctg caa tcc gcg atg acc gat cgc ggg cca gtt caa 576 Thr Gly Asp Arg Leu Gln Ser Ala Met Thr Asp Arg Gly Pro Val Gln 180 185 190 gct gac gcc tac gtt ctg gca acc ggc gct tgg tcc ggc gac tgg cta 624 Ala Asp Ala Tyr Val Leu Ala Thr Gly Ala Trp Ser Gly Asp Trp Leu 195 200 205 caa ctg ccg gtc tat ccc gtt aaa ggc caa atg ttc tcg ctg caa gct 672 Gln Leu Pro Val Tyr Pro Val Lys Gly Gln Met Phe Ser Leu Gln Ala 210 215 220 gac ccg cgt ttg ctg aac cac gtt ttg ttt ggt gag cgg gtg tat att 720 Asp Pro Arg Leu Leu Asn His Val Leu Phe Gly Glu Arg Val Tyr Ile 225 230 235 240 gtg ccg cgc cga gat ggt ctg att gtg gtc ggt gcc acc atg gaa gcg 768 Val Pro Arg Arg Asp Gly Leu Ile Val Val Gly Ala Thr Met Glu Ala 245 250 255 acg gcg gga ttc agg act ggc aac acc gct ggc ccc tta cag agc ttg 816 Thr Ala Gly Phe Arg Thr Gly Asn Thr Ala Gly Pro Leu Gln Ser Leu 260 265 270 atg gcc gag gcg atc gcc ctc gtt ccg gct ctg gcg gac tgt cca ctg 864 Met Ala Glu Ala Ile Ala Leu Val Pro Ala Leu Ala Asp Cys Pro Leu 275 280 285 gtt gaa act tgg tgg gga tac cgt ccc gcg aca cca gat gaa tgg ccg 912 Val Glu Thr Trp Trp Gly Tyr Arg Pro Ala Thr Pro Asp Glu Trp Pro 290 295 300 atc ctg ggg caa ggc ccc gct gag aac tta ttc ttg gcg acc ggc cac 960 Ile Leu Gly Gln Gly Pro Ala Glu Asn Leu Phe Leu Ala Thr Gly His 305 310 315 320 tac cgc aac ggt atg ctg ctc gcc cca att acc gct cag cta ctc gct 1008 Tyr Arg Asn Gly Met Leu Leu Ala Pro Ile Thr Ala Gln Leu Leu Ala 325 330 335 gac caa att ctc gac cac tgc acg gat caa ctg ctt cat gcc ttc cgt 1056 Asp Gln Ile Leu Asp His Cys Thr Asp Gln Leu Leu His Ala Phe Arg 340 345 350 tac gac cgc ttc tcc agc cat gac tcc agc acc cat caa ccc tta ccc 1104 Tyr Asp Arg Phe Ser Ser His Asp Ser Ser Thr His Gln Pro Leu Pro 355 360 365 gct ctt gca ggc ttg tca gcg tca acg ggt cag tga 1140 Ala Leu Ala Gly Leu Ser Ala Ser Thr Gly Gln 370 375 <210> 223 <211> 379 <212> PRT <213> Synechococcus elongatus <400> 223 Met Ala Phe Glu Val Ala Val Phe Gly Gly Gly Val Ile Gly Leu Ala 1 5 10 15 Ile Ala Leu Glu Leu Arg Ser Arg Gly Ala Met Val Gln Val Tyr Ser 20 25 30 Gln Asn Thr Gln Ala Ala Ala Gly Arg Val Ala Ala Gly Met Leu Ala 35 40 45 Pro Gln Ser Glu Gly Ile Glu Val Gly Pro Met Leu Asp Leu Gly Leu 50 55 60 Arg Ser Arg Ser Leu Tyr Ala Arg Trp Thr Gln Gln Leu Glu Gln Leu 65 70 75 80 Ser Gly Gln Asp Ser Gly Tyr Trp Pro Cys Gly Ile Leu Val Pro Leu 85 90 95 Ser Glu Ala Lys Asn Arg Asp Arg Tyr Pro His Pro Ala Glu Ser Pro 100 105 110 Gly Gln Trp Leu Ser Ala Ala Asp Leu Arg Asp Phe Gln Pro Ala Leu 115 120 125 Cys Ser Asp Leu Ile Gly Gly Trp Trp Phe Ser Gln Glu Gly Gln Val 130 135 140 Asp Ser Arg Arg Ala Leu Tyr Pro Ala Leu Arg Ala Ala Ala Ile Ala 145 150 155 160 Ser Gly Val Thr Ile His Glu Ser Val Ala Leu Arg Glu Leu Ser Val 165 170 175 Thr Gly Asp Arg Leu Gln Ser Ala Met Thr Asp Arg Gly Pro Val Gln 180 185 190 Ala Asp Ala Tyr Val Leu Ala Thr Gly Ala Trp Ser Gly Asp Trp Leu 195 200 205 Gln Leu Pro Val Tyr Pro Val Lys Gly Gln Met Phe Ser Leu Gln Ala 210 215 220 Asp Pro Arg Leu Leu Asn His Val Leu Phe Gly Glu Arg Val Tyr Ile 225 230 235 240 Val Pro Arg Arg Asp Gly Leu Ile Val Val Gly Ala Thr Met Glu Ala 245 250 255 Thr Ala Gly Phe Arg Thr Gly Asn Thr Ala Gly Pro Leu Gln Ser Leu 260 265 270 Met Ala Glu Ala Ile Ala Leu Val Pro Ala Leu Ala Asp Cys Pro Leu 275 280 285 Val Glu Thr Trp Trp Gly Tyr Arg Pro Ala Thr Pro Asp Glu Trp Pro 290 295 300 Ile Leu Gly Gln Gly Pro Ala Glu Asn Leu Phe Leu Ala Thr Gly His 305 310 315 320 Tyr Arg Asn Gly Met Leu Leu Ala Pro Ile Thr Ala Gln Leu Leu Ala 325 330 335 Asp Gln Ile Leu Asp His Cys Thr Asp Gln Leu Leu His Ala Phe Arg 340 345 350 Tyr Asp Arg Phe Ser Ser His Asp Ser Ser Thr His Gln Pro Leu Pro 355 360 365 Ala Leu Ala Gly Leu Ser Ala Ser Thr Gly Gln 370 375 <210> 224 <211> 801 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(801) <223> ThiD gene from E. coli encoding phosphohydroxymethylpyrimidine kinase <400> 224 atg aaa cga att aac gct ctg acg att gcc ggt act gat ccg agt ggt 48 Met Lys Arg Ile Asn Ala Leu Thr Ile Ala Gly Thr Asp Pro Ser Gly 1 5 10 15 ggt gcg ggg att cag gcc gat ctt aaa acc ttc tcg gca ctt ggc gct 96 Gly Ala Gly Ile Gln Ala Asp Leu Lys Thr Phe Ser Ala Leu Gly Ala 20 25 30 tat ggt tgc tca gtt att act gca ctg gtg gcg caa aat acc cgt ggc 144 Tyr Gly Cys Ser Val Ile Thr Ala Leu Val Ala Gln Asn Thr Arg Gly 35 40 45 gta cag tcg gtg tat cgc att gag cct gat ttt gtc gcc gcc cag ctc 192 Val Gln Ser Val Tyr Arg Ile Glu Pro Asp Phe Val Ala Ala Gln Leu 50 55 60 gat tcg gtg ttc agc gat gtg cga atc gat acc act aaa atc ggt atg 240 Asp Ser Val Phe Ser Asp Val Arg Ile Asp Thr Thr Lys Ile Gly Met 65 70 75 80 ctg gcg gaa acc gat att gtt gaa gcg gtg gca gaa cgg ttg caa cgt 288 Leu Ala Glu Thr Asp Ile Val Glu Ala Val Ala Glu Arg Leu Gln Arg 85 90 95 tat cag atc caa aac gtg gta ctc gac acc gtt atg ctg gca aaa agc 336 Tyr Gln Ile Gln Asn Val Val Leu Asp Thr Val Met Leu Ala Lys Ser 100 105 110 ggc gac ccg ctg ctt tca cct tcg gcg gtt gct acg ctg cgc agt cga 384 Gly Asp Pro Leu Leu Ser Pro Ser Ala Val Ala Thr Leu Arg Ser Arg 115 120 125 tta ttg cca cag gtt tca tta ata acg cca aac ttg ccc gaa gct gcc 432 Leu Leu Pro Gln Val Ser Leu Ile Thr Pro Asn Leu Pro Glu Ala Ala 130 135 140 gcc ttg ctc gac gcg cca cac gcg cgc acc gaa cag gaa atg ctg gaa 480 Ala Leu Leu Asp Ala Pro His Ala Arg Thr Glu Gln Glu Met Leu Glu 145 150 155 160 caa ggg cga tcg ctg ttg gcg atg ggc tgt ggc gca gtg cta atg aaa 528 Gln Gly Arg Ser Leu Leu Ala Met Gly Cys Gly Ala Val Leu Met Lys 165 170 175 ggt ggt cat ctg gat gat gag caa agc ccg gac tgg ctg ttt acc cgc 576 Gly Gly His Leu Asp Asp Glu Gln Ser Pro Asp Trp Leu Phe Thr Arg 180 185 190 gag ggt gaa caa cgg ttt acc gca ccg cgc att atg acc aaa aac acc 624 Glu Gly Glu Gln Arg Phe Thr Ala Pro Arg Ile Met Thr Lys Asn Thr 195 200 205 cac ggc act ggt tgt aca ctc tct gcg gcg ttg gct gca cta cgc ccg 672 His Gly Thr Gly Cys Thr Leu Ser Ala Ala Leu Ala Ala Leu Arg Pro 210 215 220 cgc cat aca aac tgg gct gac acc gta cag gag gca aaa agc tgg ctt 720 Arg His Thr Asn Trp Ala Asp Thr Val Gln Glu Ala Lys Ser Trp Leu 225 230 235 240 tca tcg gcg tta gcc cag gcc gac acg ctg gaa gtt ggt cac ggt att 768 Ser Ser Ala Leu Ala Gln Ala Asp Thr Leu Glu Val Gly His Gly Ile 245 250 255 ggt ccg gtt cac cac ttc cac gcc tgg tgg tga 801 Gly Pro Val His His Phe His Ala Trp Trp 260 265 <210> 225 <211> 266 <212> PRT <213> Escherichia coli <400> 225 Met Lys Arg Ile Asn Ala Leu Thr Ile Ala Gly Thr Asp Pro Ser Gly 1 5 10 15 Gly Ala Gly Ile Gln Ala Asp Leu Lys Thr Phe Ser Ala Leu Gly Ala 20 25 30 Tyr Gly Cys Ser Val Ile Thr Ala Leu Val Ala Gln Asn Thr Arg Gly 35 40 45 Val Gln Ser Val Tyr Arg Ile Glu Pro Asp Phe Val Ala Ala Gln Leu 50 55 60 Asp Ser Val Phe Ser Asp Val Arg Ile Asp Thr Thr Lys Ile Gly Met 65 70 75 80 Leu Ala Glu Thr Asp Ile Val Glu Ala Val Ala Glu Arg Leu Gln Arg 85 90 95 Tyr Gln Ile Gln Asn Val Val Leu Asp Thr Val Met Leu Ala Lys Ser 100 105 110 Gly Asp Pro Leu Leu Ser Pro Ser Ala Val Ala Thr Leu Arg Ser Arg 115 120 125 Leu Leu Pro Gln Val Ser Leu Ile Thr Pro Asn Leu Pro Glu Ala Ala 130 135 140 Ala Leu Leu Asp Ala Pro His Ala Arg Thr Glu Gln Glu Met Leu Glu 145 150 155 160 Gln Gly Arg Ser Leu Leu Ala Met Gly Cys Gly Ala Val Leu Met Lys 165 170 175 Gly Gly His Leu Asp Asp Glu Gln Ser Pro Asp Trp Leu Phe Thr Arg 180 185 190 Glu Gly Glu Gln Arg Phe Thr Ala Pro Arg Ile Met Thr Lys Asn Thr 195 200 205 His Gly Thr Gly Cys Thr Leu Ser Ala Ala Leu Ala Ala Leu Arg Pro 210 215 220 Arg His Thr Asn Trp Ala Asp Thr Val Gln Glu Ala Lys Ser Trp Leu 225 230 235 240 Ser Ser Ala Leu Ala Gln Ala Asp Thr Leu Glu Val Gly His Gly Ile 245 250 255 Gly Pro Val His His Phe His Ala Trp Trp 260 265 <210> 226 <211> 789 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(789) <223> ThiM gene from E. coli encoding a Hydroxyethylthiazole kinase <400> 226 atg caa gtc gac ctg ctg ggt tca gcg caa tct gcg cac gcg tta cac 48 Met Gln Val Asp Leu Leu Gly Ser Ala Gln Ser Ala His Ala Leu His 1 5 10 15 ctt ttt cac caa cat tcc cct ctt gtg cac tgc atg acc aat gat gtg 96 Leu Phe His Gln His Ser Pro Leu Val His Cys Met Thr Asn Asp Val 20 25 30 gtg caa acc ttt acc gcc aat acc ttg ctg gcg ctc ggt gca tcg cca 144 Val Gln Thr Phe Thr Ala Asn Thr Leu Leu Ala Leu Gly Ala Ser Pro 35 40 45 gcg atg gtt atc gaa acc gaa gag gcc agt cag ttt gcg gct atc gcc 192 Ala Met Val Ile Glu Thr Glu Glu Ala Ser Gln Phe Ala Ala Ile Ala 50 55 60 agt gcc ttg ttg att aac gtt ggc aca ctg acg cag cca cgc gct cag 240 Ser Ala Leu Leu Ile Asn Val Gly Thr Leu Thr Gln Pro Arg Ala Gln 65 70 75 80 gcg atg cgt gct gcc gtt gag caa gca aaa agc tct caa aca ccc tgg 288 Ala Met Arg Ala Ala Val Glu Gln Ala Lys Ser Ser Gln Thr Pro Trp 85 90 95 acg ctt gat cca gta gcg gtg ggt gcg ctc gat tat cgc cgc cat ttt 336 Thr Leu Asp Pro Val Ala Val Gly Ala Leu Asp Tyr Arg Arg His Phe 100 105 110 tgt cat gaa ctt tta tct ttt aaa ccg gca gcg ata cgt ggt aat gct 384 Cys His Glu Leu Leu Ser Phe Lys Pro Ala Ala Ile Arg Gly Asn Ala 115 120 125 tcg gaa atc atg gca tta gct ggc att gct aat ggc gga cgg gga gtg 432 Ser Glu Ile Met Ala Leu Ala Gly Ile Ala Asn Gly Gly Arg Gly Val 130 135 140 gat acc act gac gcc gca gct aac gcg ata ccc gct gca caa aca ctg 480 Asp Thr Thr Asp Ala Ala Ala Asn Ala Ile Pro Ala Ala Gln Thr Leu 145 150 155 160 gca cgg gaa act ggc gca atc gtc gtg gtc act ggc gag atg gat tat 528 Ala Arg Glu Thr Gly Ala Ile Val Val Val Thr Gly Glu Met Asp Tyr 165 170 175 gtt acc gat gga cat cgt atc att ggt att cac ggt ggt gat ccg tta 576 Val Thr Asp Gly His Arg Ile Ile Gly Ile His Gly Gly Asp Pro Leu 180 185 190 atg acc aaa gtg gta gga act ggc tgt gca tta tcg gcg gtt gtc gct 624 Met Thr Lys Val Val Gly Thr Gly Cys Ala Leu Ser Ala Val Val Ala 195 200 205 gcc tgc tgt gcg tta cca ggc gat acg ctg gaa aat gtc gca tct gcc 672 Ala Cys Cys Ala Leu Pro Gly Asp Thr Leu Glu Asn Val Ala Ser Ala 210 215 220 tgt cac tgg atg aaa caa gcc gga gaa cgc gca gtc gcc aga agc gag 720 Cys His Trp Met Lys Gln Ala Gly Glu Arg Ala Val Ala Arg Ser Glu 225 230 235 240 ggg cca ggc agt ttt gtt cca cat ttc ctt gat gcg ctc tgg caa ttg 768 Gly Pro Gly Ser Phe Val Pro His Phe Leu Asp Ala Leu Trp Gln Leu 245 250 255 acg cag gag gtg cag gca tga 789 Thr Gln Glu Val Gln Ala 260 <210> 227 <211> 262 <212> PRT <213> Escherichia coli <400> 227 Met Gln Val Asp Leu Leu Gly Ser Ala Gln Ser Ala His Ala Leu His 1 5 10 15 Leu Phe His Gln His Ser Pro Leu Val His Cys Met Thr Asn Asp Val 20 25 30 Val Gln Thr Phe Thr Ala Asn Thr Leu Leu Ala Leu Gly Ala Ser Pro 35 40 45 Ala Met Val Ile Glu Thr Glu Glu Ala Ser Gln Phe Ala Ala Ile Ala 50 55 60 Ser Ala Leu Leu Ile Asn Val Gly Thr Leu Thr Gln Pro Arg Ala Gln 65 70 75 80 Ala Met Arg Ala Ala Val Glu Gln Ala Lys Ser Ser Gln Thr Pro Trp 85 90 95 Thr Leu Asp Pro Val Ala Val Gly Ala Leu Asp Tyr Arg Arg His Phe 100 105 110 Cys His Glu Leu Leu Ser Phe Lys Pro Ala Ala Ile Arg Gly Asn Ala 115 120 125 Ser Glu Ile Met Ala Leu Ala Gly Ile Ala Asn Gly Gly Arg Gly Val 130 135 140 Asp Thr Thr Asp Ala Ala Ala Asn Ala Ile Pro Ala Ala Gln Thr Leu 145 150 155 160 Ala Arg Glu Thr Gly Ala Ile Val Val Val Thr Gly Glu Met Asp Tyr 165 170 175 Val Thr Asp Gly His Arg Ile Ile Gly Ile His Gly Gly Asp Pro Leu 180 185 190 Met Thr Lys Val Val Gly Thr Gly Cys Ala Leu Ser Ala Val Val Ala 195 200 205 Ala Cys Cys Ala Leu Pro Gly Asp Thr Leu Glu Asn Val Ala Ser Ala 210 215 220 Cys His Trp Met Lys Gln Ala Gly Glu Arg Ala Val Ala Arg Ser Glu 225 230 235 240 Gly Pro Gly Ser Phe Val Pro His Phe Leu Asp Ala Leu Trp Gln Leu 245 250 255 Thr Gln Glu Val Gln Ala 260 <210> 228 <211> 978 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(978) <223> ThiL gene from E. coli encoding thiamine-phosphate kinase (Note: mutation at nucleotides 133-135 (GGT to GAC) encodes a G133D substitution) <400> 228 atg gca tgt ggc gag ttc tcc ctg att gcc cgt tat ttt gac cgt gta 48 Met Ala Cys Gly Glu Phe Ser Leu Ile Ala Arg Tyr Phe Asp Arg Val 1 5 10 15 aga agt tct cgt ctt gat gtc gaa ctg ggc atc ggc gac gat tgc gca 96 Arg Ser Ser Arg Leu Asp Val Glu Leu Gly Ile Gly Asp Asp Cys Ala 20 25 30 ctt ctc aat atc ccc gag aaa cag acc ctg gcg atc agc act gat acg 144 Leu Leu Asn Ile Pro Glu Lys Gln Thr Leu Ala Ile Ser Thr Asp Thr 35 40 45 ctg gtg gcg ggt aac cat ttc ctc cct gat atc gat cct gct gat ctg 192 Leu Val Ala Gly Asn His Phe Leu Pro Asp Ile Asp Pro Ala Asp Leu 50 55 60 gct tat aaa gca ctg gcg gtg aac cta agc gat ctg gca gcg atg ggg 240 Ala Tyr Lys Ala Leu Ala Val Asn Leu Ser Asp Leu Ala Ala Met Gly 65 70 75 80 gcc gat ccg gcc tgg ctg acg ctg gca tta acc tta ccg gac gta gac 288 Ala Asp Pro Ala Trp Leu Thr Leu Ala Leu Thr Leu Pro Asp Val Asp 85 90 95 gaa gcg tgg ctt gag tcc ttc agc gac agt ttg ttt gat ctt ctc aat 336 Glu Ala Trp Leu Glu Ser Phe Ser Asp Ser Leu Phe Asp Leu Leu Asn 100 105 110 tat tac gat atg caa ctc att ggc ggc gat acc acg cgt ggg cca tta 384 Tyr Tyr Asp Met Gln Leu Ile Gly Gly Asp Thr Thr Arg Gly Pro Leu 115 120 125 tca atg acg ttg ggt atc cac ggc ttt gtt ccg atg gga cga gcc tta 432 Ser Met Thr Leu Gly Ile His Gly Phe Val Pro Met Gly Arg Ala Leu 130 135 140 acg cgc tct ggg gcg aaa ccg ggt gac tgg atc tat gtg acc ggt aca 480 Thr Arg Ser Gly Ala Lys Pro Gly Asp Trp Ile Tyr Val Thr Gly Thr 145 150 155 160 ccg ggc gat agc gcc gcc ggg ctg gcg att ttg caa aac cgt ttg cag 528 Pro Gly Asp Ser Ala Ala Gly Leu Ala Ile Leu Gln Asn Arg Leu Gln 165 170 175 gtt gcc gat gct aaa gat gcg gac tac ttg atc aaa cgt cat ctc cgt 576 Val Ala Asp Ala Lys Asp Ala Asp Tyr Leu Ile Lys Arg His Leu Arg 180 185 190 cca tcg ccg cgt att tta cag ggg cag gca ctg cgc gat ctg gca aat 624 Pro Ser Pro Arg Ile Leu Gln Gly Gln Ala Leu Arg Asp Leu Ala Asn 195 200 205 tca gcc atc gat ctc tct gac ggt ttg att tcc gat ctc ggg cat atc 672 Ser Ala Ile Asp Leu Ser Asp Gly Leu Ile Ser Asp Leu Gly His Ile 210 215 220 gtg aaa gcc agc gac tgc ggc gca cgt att gac ctg gca ttg ctg ccg 720 Val Lys Ala Ser Asp Cys Gly Ala Arg Ile Asp Leu Ala Leu Leu Pro 225 230 235 240 ttt tct gat gcg ctt tct cgc cat gtt gaa ccg gaa cag gcg ctg cgc 768 Phe Ser Asp Ala Leu Ser Arg His Val Glu Pro Glu Gln Ala Leu Arg 245 250 255 tgg gcg ctc tct ggc ggt gaa gat tac gag ttg tgt ttc act gtg ccg 816 Trp Ala Leu Ser Gly Gly Glu Asp Tyr Glu Leu Cys Phe Thr Val Pro 260 265 270 gaa ctg aac cgt ggc gcg ctg gat gtg gct ctc gga cac ctg ggc gta 864 Glu Leu Asn Arg Gly Ala Leu Asp Val Ala Leu Gly His Leu Gly Val 275 280 285 ccg ttt acc tgt atc ggg caa atg acc gcc gat atc gaa ggg ctt tgt 912 Pro Phe Thr Cys Ile Gly Gln Met Thr Ala Asp Ile Glu Gly Leu Cys 290 295 300 ttt att cgt gac ggc gaa cct gtt aca tta gac tgg aaa gga tat gac 960 Phe Ile Arg Asp Gly Glu Pro Val Thr Leu Asp Trp Lys Gly Tyr Asp 305 310 315 320 cat ttt gcc acg cca taa 978 His Phe Ala Thr Pro 325 <210> 229 <211> 325 <212> PRT <213> Escherichia coli <400> 229 Met Ala Cys Gly Glu Phe Ser Leu Ile Ala Arg Tyr Phe Asp Arg Val 1 5 10 15 Arg Ser Ser Arg Leu Asp Val Glu Leu Gly Ile Gly Asp Asp Cys Ala 20 25 30 Leu Leu Asn Ile Pro Glu Lys Gln Thr Leu Ala Ile Ser Thr Asp Thr 35 40 45 Leu Val Ala Gly Asn His Phe Leu Pro Asp Ile Asp Pro Ala Asp Leu 50 55 60 Ala Tyr Lys Ala Leu Ala Val Asn Leu Ser Asp Leu Ala Ala Met Gly 65 70 75 80 Ala Asp Pro Ala Trp Leu Thr Leu Ala Leu Thr Leu Pro Asp Val Asp 85 90 95 Glu Ala Trp Leu Glu Ser Phe Ser Asp Ser Leu Phe Asp Leu Leu Asn 100 105 110 Tyr Tyr Asp Met Gln Leu Ile Gly Gly Asp Thr Thr Arg Gly Pro Leu 115 120 125 Ser Met Thr Leu Gly Ile His Gly Phe Val Pro Met Gly Arg Ala Leu 130 135 140 Thr Arg Ser Gly Ala Lys Pro Gly Asp Trp Ile Tyr Val Thr Gly Thr 145 150 155 160 Pro Gly Asp Ser Ala Ala Gly Leu Ala Ile Leu Gln Asn Arg Leu Gln 165 170 175 Val Ala Asp Ala Lys Asp Ala Asp Tyr Leu Ile Lys Arg His Leu Arg 180 185 190 Pro Ser Pro Arg Ile Leu Gln Gly Gln Ala Leu Arg Asp Leu Ala Asn 195 200 205 Ser Ala Ile Asp Leu Ser Asp Gly Leu Ile Ser Asp Leu Gly His Ile 210 215 220 Val Lys Ala Ser Asp Cys Gly Ala Arg Ile Asp Leu Ala Leu Leu Pro 225 230 235 240 Phe Ser Asp Ala Leu Ser Arg His Val Glu Pro Glu Gln Ala Leu Arg 245 250 255 Trp Ala Leu Ser Gly Gly Glu Asp Tyr Glu Leu Cys Phe Thr Val Pro 260 265 270 Glu Leu Asn Arg Gly Ala Leu Asp Val Ala Leu Gly His Leu Gly Val 275 280 285 Pro Phe Thr Cys Ile Gly Gln Met Thr Ala Asp Ile Glu Gly Leu Cys 290 295 300 Phe Ile Arg Asp Gly Glu Pro Val Thr Leu Asp Trp Lys Gly Tyr Asp 305 310 315 320 His Phe Ala Thr Pro 325 <210> 230 <211> 47 <212> DNA <213> Escherichia coli <220> <221> promoter <222> (1)..(47) <223> apFAB46 promoter <400> 230 aaaaagagta ttgacttcgc atctttttgt acctataata gattcat 47 <210> 231 <211> 37 <212> DNA <213> Escherichia coli <220> <221> promoter <222> (1)..(37) <223> apFAB70 promoter <400> 231 ttgacatcgc atctttttgt acctataatg tgtggat 37 <210> 232 <211> 37 <212> DNA <213> Escherichia coli <220> <221> promoter <222> (1)..(37) <223> apFAB71 promoter <400> 232 ttgacatcgc atctttttgt acctataata gattcat 37 <210> 233 <211> 29 <212> DNA <213> Escherichia coli <220> <221> promoter <222> (1)..(29) <223> pBAD Ara promoter <400> 233 ctgacgcttt ttatcgcaac tctctactg 29 <210> 234 <211> 74 <212> DNA <213> Escherichia coli <220> <221> promoter <222> (1)..(74) <223> lac Promoter with lacO operator site <400> 234 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 60 gcataaagtg taaa 74 <210> 235 <211> 91 <212> DNA <213> Escherichia coli <220> <221> terminator <222> (1)..(91) <223> apFAB378 terminator <400> 235 gagttggtag ctcttgatcc ggcaaacaaa ccaccgttgg tagcggtggt ttttttgttt 60 gcaagcagca gattacgcgc agaaaaaaag g 91 <210> 236 <211> 91 <212> DNA <213> Escherichia coli <220> <221> terminator <222> (1)..(91) <223> apFAB377 terminator <400> 236 atgaccatct acattactga gctaataaca ggcctgctgg taatcgcagg cctttttatt 60 tgggggagag ggaagtcatg aaaaaactaa c 91 <210> 237 <211> 90 <212> DNA <213> Escherichia coli <220> <221> terminator <222> (1)..(90) <223> apFAB381 terminator <400> 237 accctcaaga gaaaatgtaa ccaactcact ggctcacctt cacgggtggg cctttcttcg 60 ttccgggcat taaccctcac taacaggaga 90 <210> 238 <211> 258 <212> DNA <213> Synthetic <220> <221> CDS <222> (1)..(258) <223> Synthetic gene encoding a E2 hybrid polypeptide (subunit of pyruvate dehydrogenase) <400> 238 atg gct atc gaa atc aaa gta ccg gac atc ggg gct gat gaa gtt gaa 48 Met Ala Ile Glu Ile Lys Val Pro Asp Ile Gly Ala Asp Glu Val Glu 1 5 10 15 atc acc gag atc ctg gtc aaa gtg ggc gac aaa gtt gaa gcc gaa cag 96 Ile Thr Glu Ile Leu Val Lys Val Gly Asp Lys Val Glu Ala Glu Gln 20 25 30 tcg ctg atc acc gta gaa ggc gac aaa gct tct atg gaa gtt ccg gcg 144 Ser Leu Ile Thr Val Glu Gly Asp Lys Ala Ser Met Glu Val Pro Ala 35 40 45 ccg ttt gca ggc gtc gtg aag gaa ctg aaa gtc aac gtt ggc gat aaa 192 Pro Phe Ala Gly Val Val Lys Glu Leu Lys Val Asn Val Gly Asp Lys 50 55 60 gtg aaa act ggc tcg ctg att atg atc ttc gaa gtt gaa ggc gca gcg 240 Val Lys Thr Gly Ser Leu Ile Met Ile Phe Glu Val Glu Gly Ala Ala 65 70 75 80 cct gcg gca gct cct gcg 258 Pro Ala Ala Ala Pro Ala 85 <210> 239 <211> 86 <212> PRT <213> Synthetic <400> 239 Met Ala Ile Glu Ile Lys Val Pro Asp Ile Gly Ala Asp Glu Val Glu 1 5 10 15 Ile Thr Glu Ile Leu Val Lys Val Gly Asp Lys Val Glu Ala Glu Gln 20 25 30 Ser Leu Ile Thr Val Glu Gly Asp Lys Ala Ser Met Glu Val Pro Ala 35 40 45 Pro Phe Ala Gly Val Val Lys Glu Leu Lys Val Asn Val Gly Asp Lys 50 55 60 Val Lys Thr Gly Ser Leu Ile Met Ile Phe Glu Val Glu Gly Ala Ala 65 70 75 80 Pro Ala Ala Ala Pro Ala 85 <210> 240 <211> 747 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(747) <223> fpr gene_E. coli encoding a flavodoxin/ferredoxin reductase <400> 240 atg gct gat tgg gta aca ggc aaa gtc act aaa gtg cag aac tgg acc 48 Met Ala Asp Trp Val Thr Gly Lys Val Thr Lys Val Gln Asn Trp Thr 1 5 10 15 gac gcc ctg ttt agt ctc acc gtt cac gcc ccc gtg ctt ccg ttt acc 96 Asp Ala Leu Phe Ser Leu Thr Val His Ala Pro Val Leu Pro Phe Thr 20 25 30 gcc ggg caa ttt acc aag ctt ggc ctt gaa atc gac ggc gaa cgc gtc 144 Ala Gly Gln Phe Thr Lys Leu Gly Leu Glu Ile Asp Gly Glu Arg Val 35 40 45 cag cgc gcc tac tcc tat gta aac tcg ccc gat aat ccc gat ctg gag 192 Gln Arg Ala Tyr Ser Tyr Val Asn Ser Pro Asp Asn Pro Asp Leu Glu 50 55 60 ttt tac ctg gtc acc gtc ccc gat ggc aaa tta agc cca cga ctg gcg 240 Phe Tyr Leu Val Thr Val Pro Asp Gly Lys Leu Ser Pro Arg Leu Ala 65 70 75 80 gca ctg aaa cca ggc gat gaa gtg cag gtg gtt agc gaa gcg gca gga 288 Ala Leu Lys Pro Gly Asp Glu Val Gln Val Val Ser Glu Ala Ala Gly 85 90 95 ttc ttt gtg ctc gat gaa gtg ccg cac tgc gaa acg cta tgg atg ctg 336 Phe Phe Val Leu Asp Glu Val Pro His Cys Glu Thr Leu Trp Met Leu 100 105 110 gca acc ggt aca gcg att ggc cct tat tta tcg att ctg caa cta ggt 384 Ala Thr Gly Thr Ala Ile Gly Pro Tyr Leu Ser Ile Leu Gln Leu Gly 115 120 125 aaa gat tta gat cgc ttc aaa aat ctg gtc ctg gtg cac gcc gca cgt 432 Lys Asp Leu Asp Arg Phe Lys Asn Leu Val Leu Val His Ala Ala Arg 130 135 140 tat gcc gcc gac tta agc tat ttg cca ctg atg cag gaa ctg gaa aaa 480 Tyr Ala Ala Asp Leu Ser Tyr Leu Pro Leu Met Gln Glu Leu Glu Lys 145 150 155 160 cgc tac gaa gga aaa ctg cgc att cag acg gtg gtc agt cgg gaa acg 528 Arg Tyr Glu Gly Lys Leu Arg Ile Gln Thr Val Val Ser Arg Glu Thr 165 170 175 gca gcg ggg tcg ctc acc gga cgg ata ccg gca tta att gaa agt ggg 576 Ala Ala Gly Ser Leu Thr Gly Arg Ile Pro Ala Leu Ile Glu Ser Gly 180 185 190 gaa ctg gaa agc acg att ggc ctg ccg atg aat aaa gaa acc agc cat 624 Glu Leu Glu Ser Thr Ile Gly Leu Pro Met Asn Lys Glu Thr Ser His 195 200 205 gtg atg ctg tgc ggc aat cca cag atg gtg cgc gat aca caa cag ttg 672 Val Met Leu Cys Gly Asn Pro Gln Met Val Arg Asp Thr Gln Gln Leu 210 215 220 ctg aaa gag acc cgg cag atg acg aaa cat tta cgt cgc cga ccg ggc 720 Leu Lys Glu Thr Arg Gln Met Thr Lys His Leu Arg Arg Arg Pro Gly 225 230 235 240 cat atg aca gcg gag cat tac tgg taa 747 His Met Thr Ala Glu His Tyr Trp 245 <210> 241 <211> 248 <212> PRT <213> Escherichia coli <400> 241 Met Ala Asp Trp Val Thr Gly Lys Val Thr Lys Val Gln Asn Trp Thr 1 5 10 15 Asp Ala Leu Phe Ser Leu Thr Val His Ala Pro Val Leu Pro Phe Thr 20 25 30 Ala Gly Gln Phe Thr Lys Leu Gly Leu Glu Ile Asp Gly Glu Arg Val 35 40 45 Gln Arg Ala Tyr Ser Tyr Val Asn Ser Pro Asp Asn Pro Asp Leu Glu 50 55 60 Phe Tyr Leu Val Thr Val Pro Asp Gly Lys Leu Ser Pro Arg Leu Ala 65 70 75 80 Ala Leu Lys Pro Gly Asp Glu Val Gln Val Val Ser Glu Ala Ala Gly 85 90 95 Phe Phe Val Leu Asp Glu Val Pro His Cys Glu Thr Leu Trp Met Leu 100 105 110 Ala Thr Gly Thr Ala Ile Gly Pro Tyr Leu Ser Ile Leu Gln Leu Gly 115 120 125 Lys Asp Leu Asp Arg Phe Lys Asn Leu Val Leu Val His Ala Ala Arg 130 135 140 Tyr Ala Ala Asp Leu Ser Tyr Leu Pro Leu Met Gln Glu Leu Glu Lys 145 150 155 160 Arg Tyr Glu Gly Lys Leu Arg Ile Gln Thr Val Val Ser Arg Glu Thr 165 170 175 Ala Ala Gly Ser Leu Thr Gly Arg Ile Pro Ala Leu Ile Glu Ser Gly 180 185 190 Glu Leu Glu Ser Thr Ile Gly Leu Pro Met Asn Lys Glu Thr Ser His 195 200 205 Val Met Leu Cys Gly Asn Pro Gln Met Val Arg Asp Thr Gln Gln Leu 210 215 220 Leu Lys Glu Thr Arg Gln Met Thr Lys His Leu Arg Arg Arg Pro Gly 225 230 235 240 His Met Thr Ala Glu His Tyr Trp 245 <210> 242 <211> 999 <212> DNA <213> Bacillus subtilis <220> <221> CDS <222> (1)..(999) <223> YumC gene_B. subtilis encoding a flavodoxin/ferredoxin reductase <400> 242 atg cga gag gat aca aag gtt tat gat att aca att ata ggc ggg gga 48 Met Arg Glu Asp Thr Lys Val Tyr Asp Ile Thr Ile Ile Gly Gly Gly 1 5 10 15 ccg gtc ggc tta ttc acc gct ttt tac ggc ggg atg aga cag gca agc 96 Pro Val Gly Leu Phe Thr Ala Phe Tyr Gly Gly Met Arg Gln Ala Ser 20 25 30 gtc aaa att atc gaa agc ctg cct cag ctc ggc gga cag ctt agc gcc 144 Val Lys Ile Ile Glu Ser Leu Pro Gln Leu Gly Gly Gln Leu Ser Ala 35 40 45 cta tac cct gag aag tat ata tat gat gta gcg gga ttc ccg aaa atc 192 Leu Tyr Pro Glu Lys Tyr Ile Tyr Asp Val Ala Gly Phe Pro Lys Ile 50 55 60 cgc gcg caa gag ctt atc aat aac cta aaa gag caa atg gcg aaa ttc 240 Arg Ala Gln Glu Leu Ile Asn Asn Leu Lys Glu Gln Met Ala Lys Phe 65 70 75 80 gac caa acc att tgt ctg gag caa gcg gtt gaa tct gtt gag aaa caa 288 Asp Gln Thr Ile Cys Leu Glu Gln Ala Val Glu Ser Val Glu Lys Gln 85 90 95 gcg gac ggc gtg ttt aag ctt gta aca aat gaa gaa acc cac tac tct 336 Ala Asp Gly Val Phe Lys Leu Val Thr Asn Glu Glu Thr His Tyr Ser 100 105 110 aaa acg gtc atc ata act gca gga aac ggc gca ttc aaa ccg aga aag 384 Lys Thr Val Ile Ile Thr Ala Gly Asn Gly Ala Phe Lys Pro Arg Lys 115 120 125 ctg gaa ctt gaa aat gcc gag cag tat gaa ggc aaa aac ctc cat tac 432 Leu Glu Leu Glu Asn Ala Glu Gln Tyr Glu Gly Lys Asn Leu His Tyr 130 135 140 ttc gtt gat gat ctg caa aaa ttc gcc ggc aga cgc gtt gcg atc ctt 480 Phe Val Asp Asp Leu Gln Lys Phe Ala Gly Arg Arg Val Ala Ile Leu 145 150 155 160 ggc ggt gga gat tcc gcg gtt gac tgg gcg ctt atg ctt gag cca atc 528 Gly Gly Gly Asp Ser Ala Val Asp Trp Ala Leu Met Leu Glu Pro Ile 165 170 175 gca aaa gaa gta tcg atc att cac cgc cgc gac aag ttc cga gcg cac 576 Ala Lys Glu Val Ser Ile Ile His Arg Arg Asp Lys Phe Arg Ala His 180 185 190 gag cac agt gtg gaa aac ctt cat gcg tcg aag gtt aat gtc ctg aca 624 Glu His Ser Val Glu Asn Leu His Ala Ser Lys Val Asn Val Leu Thr 195 200 205 cca ttc gtc cct gcg gag ctg atc ggc gaa gac aaa atc gaa cag cta 672 Pro Phe Val Pro Ala Glu Leu Ile Gly Glu Asp Lys Ile Glu Gln Leu 210 215 220 gtg ctt gaa gaa gtg aaa ggc gac cgc aaa gag att tta gaa att gat 720 Val Leu Glu Glu Val Lys Gly Asp Arg Lys Glu Ile Leu Glu Ile Asp 225 230 235 240 gac tta atc gtc aac tac ggt ttc gtt tca tct ctt gga ccg atc aaa 768 Asp Leu Ile Val Asn Tyr Gly Phe Val Ser Ser Leu Gly Pro Ile Lys 245 250 255 aac tgg ggc ctg gac atc gag aaa aat tcc att gtc gtg aaa tca aca 816 Asn Trp Gly Leu Asp Ile Glu Lys Asn Ser Ile Val Val Lys Ser Thr 260 265 270 atg gaa aca aat atc gaa ggc ttc ttt gca gca ggt gac att tgt aca 864 Met Glu Thr Asn Ile Glu Gly Phe Phe Ala Ala Gly Asp Ile Cys Thr 275 280 285 tac gaa gga aaa gtc aac ctg att gcc agc ggc ttc ggc gag gca ccg 912 Tyr Glu Gly Lys Val Asn Leu Ile Ala Ser Gly Phe Gly Glu Ala Pro 290 295 300 aca gca gtg aac aac gcc aag gct tac atg gac ccg aaa gcc cgc gta 960 Thr Ala Val Asn Asn Ala Lys Ala Tyr Met Asp Pro Lys Ala Arg Val 305 310 315 320 cag cct ctt cac tca aca agt ctt ttt gaa aat aaa taa 999 Gln Pro Leu His Ser Thr Ser Leu Phe Glu Asn Lys 325 330 <210> 243 <211> 332 <212> PRT <213> Bacillus subtilis <400> 243 Met Arg Glu Asp Thr Lys Val Tyr Asp Ile Thr Ile Ile Gly Gly Gly 1 5 10 15 Pro Val Gly Leu Phe Thr Ala Phe Tyr Gly Gly Met Arg Gln Ala Ser 20 25 30 Val Lys Ile Ile Glu Ser Leu Pro Gln Leu Gly Gly Gln Leu Ser Ala 35 40 45 Leu Tyr Pro Glu Lys Tyr Ile Tyr Asp Val Ala Gly Phe Pro Lys Ile 50 55 60 Arg Ala Gln Glu Leu Ile Asn Asn Leu Lys Glu Gln Met Ala Lys Phe 65 70 75 80 Asp Gln Thr Ile Cys Leu Glu Gln Ala Val Glu Ser Val Glu Lys Gln 85 90 95 Ala Asp Gly Val Phe Lys Leu Val Thr Asn Glu Glu Thr His Tyr Ser 100 105 110 Lys Thr Val Ile Ile Thr Ala Gly Asn Gly Ala Phe Lys Pro Arg Lys 115 120 125 Leu Glu Leu Glu Asn Ala Glu Gln Tyr Glu Gly Lys Asn Leu His Tyr 130 135 140 Phe Val Asp Asp Leu Gln Lys Phe Ala Gly Arg Arg Val Ala Ile Leu 145 150 155 160 Gly Gly Gly Asp Ser Ala Val Asp Trp Ala Leu Met Leu Glu Pro Ile 165 170 175 Ala Lys Glu Val Ser Ile Ile His Arg Arg Asp Lys Phe Arg Ala His 180 185 190 Glu His Ser Val Glu Asn Leu His Ala Ser Lys Val Asn Val Leu Thr 195 200 205 Pro Phe Val Pro Ala Glu Leu Ile Gly Glu Asp Lys Ile Glu Gln Leu 210 215 220 Val Leu Glu Glu Val Lys Gly Asp Arg Lys Glu Ile Leu Glu Ile Asp 225 230 235 240 Asp Leu Ile Val Asn Tyr Gly Phe Val Ser Ser Leu Gly Pro Ile Lys 245 250 255 Asn Trp Gly Leu Asp Ile Glu Lys Asn Ser Ile Val Val Lys Ser Thr 260 265 270 Met Glu Thr Asn Ile Glu Gly Phe Phe Ala Ala Gly Asp Ile Cys Thr 275 280 285 Tyr Glu Gly Lys Val Asn Leu Ile Ala Ser Gly Phe Gly Glu Ala Pro 290 295 300 Thr Ala Val Asn Asn Ala Lys Ala Tyr Met Asp Pro Lys Ala Arg Val 305 310 315 320 Gln Pro Leu His Ser Thr Ser Leu Phe Glu Asn Lys 325 330 <210> 244 <211> 780 <212> DNA <213> Pseudomonas pudita <220> <221> CDS <222> (1)..(780) <223> fpr-1 gene from Pseudomonas putida KT2440 encoding a flavodoxin/ferredoxin reductase <400> 244 atg agc aac atg aac cac gaa cgt gtc ctc agt gtg cac cac tgg aac 48 Met Ser Asn Met Asn His Glu Arg Val Leu Ser Val His His Trp Asn 1 5 10 15 gac acc ctg ttc agc ttc aag tgc acc cgc gac ccg ggc ctg cgc ttc 96 Asp Thr Leu Phe Ser Phe Lys Cys Thr Arg Asp Pro Gly Leu Arg Phe 20 25 30 gag aac ggt cag ttc gtg atg atc ggc ctg cag cag gac aac ggc cgt 144 Glu Asn Gly Gln Phe Val Met Ile Gly Leu Gln Gln Asp Asn Gly Arg 35 40 45 ccg ctc atg cgt gcc tac tcc atc gct tcg cca aac tgg gaa gag cac 192 Pro Leu Met Arg Ala Tyr Ser Ile Ala Ser Pro Asn Trp Glu Glu His 50 55 60 ctt gaa ttc ttc agc atc aag gtg ccg gac ggc ccg ctg acc tcg cag 240 Leu Glu Phe Phe Ser Ile Lys Val Pro Asp Gly Pro Leu Thr Ser Gln 65 70 75 80 ctg cag cac ctg aag gaa ggc gat gag atc atc atc agc aag aag cct 288 Leu Gln His Leu Lys Glu Gly Asp Glu Ile Ile Ile Ser Lys Lys Pro 85 90 95 acc ggc acc ctg gtc ctc gac gac ctg aat cct ggc aag cac ctg tac 336 Thr Gly Thr Leu Val Leu Asp Asp Leu Asn Pro Gly Lys His Leu Tyr 100 105 110 ctg ctg agc acc ggc act ggt ctg gcg ccg ttc atg agc gtc atc cag 384 Leu Leu Ser Thr Gly Thr Gly Leu Ala Pro Phe Met Ser Val Ile Gln 115 120 125 gac ccg gaa acc tac gag cgc ttt gaa aaa gtg atc ctg gtg cac ggc 432 Asp Pro Glu Thr Tyr Glu Arg Phe Glu Lys Val Ile Leu Val His Gly 130 135 140 gtg cgc tat gtg aac gaa gtg gcc tac cgc gag ttc atc acc gag cac 480 Val Arg Tyr Val Asn Glu Val Ala Tyr Arg Glu Phe Ile Thr Glu His 145 150 155 160 ctg ccg cag aac gag ttc ttc ggt gag tcg gtt cgc gac aag ctg atc 528 Leu Pro Gln Asn Glu Phe Phe Gly Glu Ser Val Arg Asp Lys Leu Ile 165 170 175 tac tac ccg acc gtg acc cgc gag ccg ttc gaa aac cag ggc cgt ctg 576 Tyr Tyr Pro Thr Val Thr Arg Glu Pro Phe Glu Asn Gln Gly Arg Leu 180 185 190 acc gac ctg atg cgc agc ggc aag ctg ttc agc gac atc ggc ctg ccg 624 Thr Asp Leu Met Arg Ser Gly Lys Leu Phe Ser Asp Ile Gly Leu Pro 195 200 205 ccg atc aac ccg caa gac gac cgc gcg atg atc tgc ggc agc ccg agc 672 Pro Ile Asn Pro Gln Asp Asp Arg Ala Met Ile Cys Gly Ser Pro Ser 210 215 220 atg ctc gac gag acc agc gaa gtg ctg gac agc ttc ggc ctg aag atc 720 Met Leu Asp Glu Thr Ser Glu Val Leu Asp Ser Phe Gly Leu Lys Ile 225 230 235 240 tcc ccg cgc atg cgc gag ccg ggt gac tac ctg atc gaa cgt gcc ttc 768 Ser Pro Arg Met Arg Glu Pro Gly Asp Tyr Leu Ile Glu Arg Ala Phe 245 250 255 gtc gag aag taa 780 Val Glu Lys <210> 245 <211> 259 <212> PRT <213> Pseudomonas pudita <400> 245 Met Ser Asn Met Asn His Glu Arg Val Leu Ser Val His His Trp Asn 1 5 10 15 Asp Thr Leu Phe Ser Phe Lys Cys Thr Arg Asp Pro Gly Leu Arg Phe 20 25 30 Glu Asn Gly Gln Phe Val Met Ile Gly Leu Gln Gln Asp Asn Gly Arg 35 40 45 Pro Leu Met Arg Ala Tyr Ser Ile Ala Ser Pro Asn Trp Glu Glu His 50 55 60 Leu Glu Phe Phe Ser Ile Lys Val Pro Asp Gly Pro Leu Thr Ser Gln 65 70 75 80 Leu Gln His Leu Lys Glu Gly Asp Glu Ile Ile Ile Ser Lys Lys Pro 85 90 95 Thr Gly Thr Leu Val Leu Asp Asp Leu Asn Pro Gly Lys His Leu Tyr 100 105 110 Leu Leu Ser Thr Gly Thr Gly Leu Ala Pro Phe Met Ser Val Ile Gln 115 120 125 Asp Pro Glu Thr Tyr Glu Arg Phe Glu Lys Val Ile Leu Val His Gly 130 135 140 Val Arg Tyr Val Asn Glu Val Ala Tyr Arg Glu Phe Ile Thr Glu His 145 150 155 160 Leu Pro Gln Asn Glu Phe Phe Gly Glu Ser Val Arg Asp Lys Leu Ile 165 170 175 Tyr Tyr Pro Thr Val Thr Arg Glu Pro Phe Glu Asn Gln Gly Arg Leu 180 185 190 Thr Asp Leu Met Arg Ser Gly Lys Leu Phe Ser Asp Ile Gly Leu Pro 195 200 205 Pro Ile Asn Pro Gln Asp Asp Arg Ala Met Ile Cys Gly Ser Pro Ser 210 215 220 Met Leu Asp Glu Thr Ser Glu Val Leu Asp Ser Phe Gly Leu Lys Ile 225 230 235 240 Ser Pro Arg Met Arg Glu Pro Gly Asp Tyr Leu Ile Glu Arg Ala Phe 245 250 255 Val Glu Lys <210> 246 <211> 936 <212> DNA <213> Streptomyces venezuelae <220> <221> CDS <222> (1)..(936) <223> SVEN_0113 gene from Streptomyces venezuelae encoding a flavodoxin/ferredoxin reductase <400> 246 atg agc gag aac ccg ctg caa ctg atc gtc cac cgc atg aca cgg gag 48 Met Ser Glu Asn Pro Leu Gln Leu Ile Val His Arg Met Thr Arg Glu 1 5 10 15 gcc gag ggc gta ctg tcc gtc gaa ctc gcc cac ccc gac ggc aag ccg 96 Ala Glu Gly Val Leu Ser Val Glu Leu Ala His Pro Asp Gly Lys Pro 20 25 30 ctg ccc gcc tgg acg ccg ggc gcc cac atc gac gtc cac gtc ggg ggc 144 Leu Pro Ala Trp Thr Pro Gly Ala His Ile Asp Val His Val Gly Gly 35 40 45 cac gtc cgc cag tac agc ctg tgc ggc gac ccg cac gac cag ggc gcg 192 His Val Arg Gln Tyr Ser Leu Cys Gly Asp Pro His Asp Gln Gly Ala 50 55 60 tac cgg atc ggc gtc ctc gac gaa ccc gcc tca cgc ggc ggt tcg cgc 240 Tyr Arg Ile Gly Val Leu Asp Glu Pro Ala Ser Arg Gly Gly Ser Arg 65 70 75 80 ttc gtg cac acc gca ctg cgc ccc ggc cag acc ctc acg gtc tcc gca 288 Phe Val His Thr Ala Leu Arg Pro Gly Gln Thr Leu Thr Val Ser Ala 85 90 95 ccc cgc aac cac ttc gcc ctc gag gac gcc gcc gcg tac gtc ctc gtc 336 Pro Arg Asn His Phe Ala Leu Glu Asp Ala Ala Ala Tyr Val Leu Val 100 105 110 gcc ggc ggc atc ggc atc acg ccc ctg ctc gcc atg gcc cgc gag gcg 384 Ala Gly Gly Ile Gly Ile Thr Pro Leu Leu Ala Met Ala Arg Glu Ala 115 120 125 gcc cgc cgg ggc gcc gag tgg cgc ctg gtc tac ggc ggc cgg agc cgg 432 Ala Arg Arg Gly Ala Glu Trp Arg Leu Val Tyr Gly Gly Arg Ser Arg 130 135 140 gcg tcg atg gcc ttc acc gcc gaa ctg gcc ctg ctc ggc ggc gag gtg 480 Ala Ser Met Ala Phe Thr Ala Glu Leu Ala Leu Leu Gly Gly Glu Val 145 150 155 160 acc ctc gtc ccg cag gac gaa cgc ggc cac atc gac ctg gac gcc gag 528 Thr Leu Val Pro Gln Asp Glu Arg Gly His Ile Asp Leu Asp Ala Glu 165 170 175 ctg tcc cgg ctg ccc gac ggc gcc ctc gtc tac gcc tgc ggc ccg gaa 576 Leu Ser Arg Leu Pro Asp Gly Ala Leu Val Tyr Ala Cys Gly Pro Glu 180 185 190 ccc ctc ctc gcg gcc gtc gag gaa cgc tgt ccg caa gga cag ctg cgc 624 Pro Leu Leu Ala Ala Val Glu Glu Arg Cys Pro Gln Gly Gln Leu Arg 195 200 205 acc gaa cgg ttc acc gcc ccc acc gtc gaa cgc gca gaa gac gac gga 672 Thr Glu Arg Phe Thr Ala Pro Thr Val Glu Arg Ala Glu Asp Asp Gly 210 215 220 gag ttc gag gtc gag tgc cgc acc tcg ggc ctg acg ctc cgg gtc gac 720 Glu Phe Glu Val Glu Cys Arg Thr Ser Gly Leu Thr Leu Arg Val Asp 225 230 235 240 gca cac tcc tcg atc ctc gac gcc gcc gag aac gcc ggg atc gcc gtc 768 Ala His Ser Ser Ile Leu Asp Ala Ala Glu Asn Ala Gly Ile Ala Val 245 250 255 gac agc tcc tgc cgc gac ggc atc tgc ggc tcc tgc gag acc cgc gtc 816 Asp Ser Ser Cys Arg Asp Gly Ile Cys Gly Ser Cys Glu Thr Arg Val 260 265 270 ctc gaa ggc acc ccg gac cac cgc gac ttc ctc ctc agc gag gcg gaa 864 Leu Glu Gly Thr Pro Asp His Arg Asp Phe Leu Leu Ser Glu Ala Glu 275 280 285 cag gcc gcc ggc gcc acc atg atg atc tgc gtc tcg cgg tgc gcc tcc 912 Gln Ala Ala Gly Ala Thr Met Met Ile Cys Val Ser Arg Cys Ala Ser 290 295 300 ggc cgg ctc gtc ctc gac ctg tga 936 Gly Arg Leu Val Leu Asp Leu 305 310 <210> 247 <211> 311 <212> PRT <213> Streptomyces venezuelae <400> 247 Met Ser Glu Asn Pro Leu Gln Leu Ile Val His Arg Met Thr Arg Glu 1 5 10 15 Ala Glu Gly Val Leu Ser Val Glu Leu Ala His Pro Asp Gly Lys Pro 20 25 30 Leu Pro Ala Trp Thr Pro Gly Ala His Ile Asp Val His Val Gly Gly 35 40 45 His Val Arg Gln Tyr Ser Leu Cys Gly Asp Pro His Asp Gln Gly Ala 50 55 60 Tyr Arg Ile Gly Val Leu Asp Glu Pro Ala Ser Arg Gly Gly Ser Arg 65 70 75 80 Phe Val His Thr Ala Leu Arg Pro Gly Gln Thr Leu Thr Val Ser Ala 85 90 95 Pro Arg Asn His Phe Ala Leu Glu Asp Ala Ala Ala Tyr Val Leu Val 100 105 110 Ala Gly Gly Ile Gly Ile Thr Pro Leu Leu Ala Met Ala Arg Glu Ala 115 120 125 Ala Arg Arg Gly Ala Glu Trp Arg Leu Val Tyr Gly Gly Arg Ser Arg 130 135 140 Ala Ser Met Ala Phe Thr Ala Glu Leu Ala Leu Leu Gly Gly Glu Val 145 150 155 160 Thr Leu Val Pro Gln Asp Glu Arg Gly His Ile Asp Leu Asp Ala Glu 165 170 175 Leu Ser Arg Leu Pro Asp Gly Ala Leu Val Tyr Ala Cys Gly Pro Glu 180 185 190 Pro Leu Leu Ala Ala Val Glu Glu Arg Cys Pro Gln Gly Gln Leu Arg 195 200 205 Thr Glu Arg Phe Thr Ala Pro Thr Val Glu Arg Ala Glu Asp Asp Gly 210 215 220 Glu Phe Glu Val Glu Cys Arg Thr Ser Gly Leu Thr Leu Arg Val Asp 225 230 235 240 Ala His Ser Ser Ile Leu Asp Ala Ala Glu Asn Ala Gly Ile Ala Val 245 250 255 Asp Ser Ser Cys Arg Asp Gly Ile Cys Gly Ser Cys Glu Thr Arg Val 260 265 270 Leu Glu Gly Thr Pro Asp His Arg Asp Phe Leu Leu Ser Glu Ala Glu 275 280 285 Gln Ala Ala Gly Ala Thr Met Met Ile Cys Val Ser Arg Cys Ala Ser 290 295 300 Gly Arg Leu Val Leu Asp Leu 305 310 <210> 248 <211> 978 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(978) <223> Cgl2384 gene from Corynebacterium glutamicum encoding a flavodoxin/ferredoxin reductase <400> 248 atg aac tcg caa tgg caa gat gca cat gtt gtt tcc agc gaa atc atc 48 Met Asn Ser Gln Trp Gln Asp Ala His Val Val Ser Ser Glu Ile Ile 1 5 10 15 gct gca gac att cgg cga ata gaa cta tcc ccg aaa ttt gcg att cca 96 Ala Ala Asp Ile Arg Arg Ile Glu Leu Ser Pro Lys Phe Ala Ile Pro 20 25 30 gta aaa ccc ggc gaa cat ctc aag atc atg gtg ccc cta aaa act gga 144 Val Lys Pro Gly Glu His Leu Lys Ile Met Val Pro Leu Lys Thr Gly 35 40 45 cag gaa aag aga tcg tac tcc atc gtt gac gct cgt cac gac ggt tcg 192 Gln Glu Lys Arg Ser Tyr Ser Ile Val Asp Ala Arg His Asp Gly Ser 50 55 60 act ctc gcc ctg agc gta ctc aaa acc aga aac tcc cgt gga gga tct 240 Thr Leu Ala Leu Ser Val Leu Lys Thr Arg Asn Ser Arg Gly Gly Ser 65 70 75 80 gag ttc atg cat acg ctt cga gct gga gac aca gtt act gtc tcc agg 288 Glu Phe Met His Thr Leu Arg Ala Gly Asp Thr Val Thr Val Ser Arg 85 90 95 ccg tct cag gat ttt cct ctc cgc gtg ggt gcg cct gag tat gta ctt 336 Pro Ser Gln Asp Phe Pro Leu Arg Val Gly Ala Pro Glu Tyr Val Leu 100 105 110 gtt gcc ggc gga att gga atc aca gcg atc cgt tca atg gca tct tta 384 Val Ala Gly Gly Ile Gly Ile Thr Ala Ile Arg Ser Met Ala Ser Leu 115 120 125 tta aag aaa ttg gga gcg aac tac cgc atc cat ttc gca gca cgc agc 432 Leu Lys Lys Leu Gly Ala Asn Tyr Arg Ile His Phe Ala Ala Arg Ser 130 135 140 ctt gat gcc atg gct tac aaa gat gag ctc gtg gca gaa cac ggc gac 480 Leu Asp Ala Met Ala Tyr Lys Asp Glu Leu Val Ala Glu His Gly Asp 145 150 155 160 aag ctg cac ctg cat cta gat tct gaa ggc acc acc atc gat gtc cca 528 Lys Leu His Leu His Leu Asp Ser Glu Gly Thr Thr Ile Asp Val Pro 165 170 175 gca ttg atc gaa acc tta aac ccc cac act gag ctt tat atg tgc ggc 576 Ala Leu Ile Glu Thr Leu Asn Pro His Thr Glu Leu Tyr Met Cys Gly 180 185 190 ccc atc cgc ttg atg gat gcc atc cgg cgc gca tgg aac acc cgc gga 624 Pro Ile Arg Leu Met Asp Ala Ile Arg Arg Ala Trp Asn Thr Arg Gly 195 200 205 ctt gac ccc acc aat ctg cgt ttc gaa acg ttt gga aac agt gga tgg 672 Leu Asp Pro Thr Asn Leu Arg Phe Glu Thr Phe Gly Asn Ser Gly Trp 210 215 220 ttc tcc cca gag gtt ttc cac atc caa gta cca gag ctg ggg ctt cac 720 Phe Ser Pro Glu Val Phe His Ile Gln Val Pro Glu Leu Gly Leu His 225 230 235 240 gcc aca gtc aac aag gat gaa agc atg ctg gag gct ttg caa aag gct 768 Ala Thr Val Asn Lys Asp Glu Ser Met Leu Glu Ala Leu Gln Lys Ala 245 250 255 ggg gcg aat atg atg ttt gat tgt cga aaa ggc gaa tgt ggt ttg tgc 816 Gly Ala Asn Met Met Phe Asp Cys Arg Lys Gly Glu Cys Gly Leu Cys 260 265 270 cag gtt cgc gtt cta gaa gtc gat ggc cag gtt gat cac cgc gat gtg 864 Gln Val Arg Val Leu Glu Val Asp Gly Gln Val Asp His Arg Asp Val 275 280 285 ttc ttc tct gat cgt caa aaa gaa tcc gac gca aag gca tgc gcc tgc 912 Phe Phe Ser Asp Arg Gln Lys Glu Ser Asp Ala Lys Ala Cys Ala Cys 290 295 300 gtg tct cga gta gtc tcc tcc cct tcc tcg tcc cca acc tcg acc att 960 Val Ser Arg Val Val Ser Ser Pro Ser Ser Ser Pro Thr Ser Thr Ile 305 310 315 320 acg gtc gcc ctc tcc taa 978 Thr Val Ala Leu Ser 325 <210> 249 <211> 325 <212> PRT <213> Corynebacterium glutamicum <400> 249 Met Asn Ser Gln Trp Gln Asp Ala His Val Val Ser Ser Glu Ile Ile 1 5 10 15 Ala Ala Asp Ile Arg Arg Ile Glu Leu Ser Pro Lys Phe Ala Ile Pro 20 25 30 Val Lys Pro Gly Glu His Leu Lys Ile Met Val Pro Leu Lys Thr Gly 35 40 45 Gln Glu Lys Arg Ser Tyr Ser Ile Val Asp Ala Arg His Asp Gly Ser 50 55 60 Thr Leu Ala Leu Ser Val Leu Lys Thr Arg Asn Ser Arg Gly Gly Ser 65 70 75 80 Glu Phe Met His Thr Leu Arg Ala Gly Asp Thr Val Thr Val Ser Arg 85 90 95 Pro Ser Gln Asp Phe Pro Leu Arg Val Gly Ala Pro Glu Tyr Val Leu 100 105 110 Val Ala Gly Gly Ile Gly Ile Thr Ala Ile Arg Ser Met Ala Ser Leu 115 120 125 Leu Lys Lys Leu Gly Ala Asn Tyr Arg Ile His Phe Ala Ala Arg Ser 130 135 140 Leu Asp Ala Met Ala Tyr Lys Asp Glu Leu Val Ala Glu His Gly Asp 145 150 155 160 Lys Leu His Leu His Leu Asp Ser Glu Gly Thr Thr Ile Asp Val Pro 165 170 175 Ala Leu Ile Glu Thr Leu Asn Pro His Thr Glu Leu Tyr Met Cys Gly 180 185 190 Pro Ile Arg Leu Met Asp Ala Ile Arg Arg Ala Trp Asn Thr Arg Gly 195 200 205 Leu Asp Pro Thr Asn Leu Arg Phe Glu Thr Phe Gly Asn Ser Gly Trp 210 215 220 Phe Ser Pro Glu Val Phe His Ile Gln Val Pro Glu Leu Gly Leu His 225 230 235 240 Ala Thr Val Asn Lys Asp Glu Ser Met Leu Glu Ala Leu Gln Lys Ala 245 250 255 Gly Ala Asn Met Met Phe Asp Cys Arg Lys Gly Glu Cys Gly Leu Cys 260 265 270 Gln Val Arg Val Leu Glu Val Asp Gly Gln Val Asp His Arg Asp Val 275 280 285 Phe Phe Ser Asp Arg Gln Lys Glu Ser Asp Ala Lys Ala Cys Ala Cys 290 295 300 Val Ser Arg Val Val Ser Ser Pro Ser Ser Ser Pro Thr Ser Thr Ile 305 310 315 320 Thr Val Ala Leu Ser 325 <210> 250 <211> 921 <212> DNA <213> Sphingobacterium sp <220> <221> CDS <222> (1)..(921) <223> SJN15614.1 from Sphingobacterium sp. JB170 encoding a flavodoxin/ferredoxin reductase <400> 250 atg ttt ggc gat cgc gag gtt cgt aga tcg tat tcc ttt agt agc tcg 48 Met Phe Gly Asp Arg Glu Val Arg Arg Ser Tyr Ser Phe Ser Ser Ser 1 5 10 15 cct gca gtt gtt gag ccg cta gcc att acc gta aaa aga gtg gat aat 96 Pro Ala Val Val Glu Pro Leu Ala Ile Thr Val Lys Arg Val Asp Asn 20 25 30 ggg gaa att tcc cgc ctg ttg cat cat cgt aca cgc gtt ggg gat ctt 144 Gly Glu Ile Ser Arg Leu Leu His His Arg Thr Arg Val Gly Asp Leu 35 40 45 gtt gat gtt cta gcc ccg cag gga tta ttt aca tac gaa cct gac ccc 192 Val Asp Val Leu Ala Pro Gln Gly Leu Phe Thr Tyr Glu Pro Asp Pro 50 55 60 act aca gct cga aca tta ttt tta ttt ggc gcc ggc gtt ggt gtt act 240 Thr Thr Ala Arg Thr Leu Phe Leu Phe Gly Ala Gly Val Gly Val Thr 65 70 75 80 ccg tta ttt tcc atc ctg aaa act gcg ctg tcc aca gaa ccc aaa acg 288 Pro Leu Phe Ser Ile Leu Lys Thr Ala Leu Ser Thr Glu Pro Lys Thr 85 90 95 aag gtt gtc ctc att tat agc aac agt tca ccc gat agg aca gtt ttt 336 Lys Val Val Leu Ile Tyr Ser Asn Ser Ser Pro Asp Arg Thr Val Phe 100 105 110 aaa gtt gaa ctt gaa cat tgg caa caa ctg tat gcc gat cgg ctt gaa 384 Lys Val Glu Leu Glu His Trp Gln Gln Leu Tyr Ala Asp Arg Leu Glu 115 120 125 att ata tgg att tac tcc aat tca aaa aat ctg tta aat gca cac cta 432 Ile Ile Trp Ile Tyr Ser Asn Ser Lys Asn Leu Leu Asn Ala His Leu 130 135 140 aac cgc gag aac tta tta cgc ttt gtc aat gaa cgc atg cct gag gat 480 Asn Arg Glu Asn Leu Leu Arg Phe Val Asn Glu Arg Met Pro Glu Asp 145 150 155 160 aac aat gct ata ttt ttc acc tgt ggt ccg gta ttt tac atg gac tta 528 Asn Asn Ala Ile Phe Phe Thr Cys Gly Pro Val Phe Tyr Met Asp Leu 165 170 175 gta cgc ttc acg tta ctc ggt ctt gga atc ccc gac gag gat atc cgc 576 Val Arg Phe Thr Leu Leu Gly Leu Gly Ile Pro Asp Glu Asp Ile Arg 180 185 190 aag gag aca ttt cat ttt cct gaa gaa gaa gat gat gaa gat gag aaa 624 Lys Glu Thr Phe His Phe Pro Glu Glu Glu Asp Asp Glu Asp Glu Lys 195 200 205 gaa gac gat ccg gtt gat acg aca gcg tac aac ata ttg ctc agg ttt 672 Glu Asp Asp Pro Val Asp Thr Thr Ala Tyr Asn Ile Leu Leu Arg Phe 210 215 220 caa gga caa gaa tac ccg ttg aca att cca tac aac aaa aca atc ttg 720 Gln Gly Gln Glu Tyr Pro Leu Thr Ile Pro Tyr Asn Lys Thr Ile Leu 225 230 235 240 cag gcc gga ctg gat aat aat att aaa ctt ccc tat tct tgt aaa tcg 768 Gln Ala Gly Leu Asp Asn Asn Ile Lys Leu Pro Tyr Ser Cys Lys Ser 245 250 255 ggg atg tgt agt act tgt atc tca caa tgt tcc agc ggg tcc gtc cgg 816 Gly Met Cys Ser Thr Cys Ile Ser Gln Cys Ser Ser Gly Ser Val Arg 260 265 270 atg gat tac aat gag gtt cta aca gat cgt gag gtt gaa aat ggc cgt 864 Met Asp Tyr Asn Glu Val Leu Thr Asp Arg Glu Val Glu Asn Gly Arg 275 280 285 tgt ttg att tgt act tcc cac ccg tta gaa gat ggt acg acg att gat 912 Cys Leu Ile Cys Thr Ser His Pro Leu Glu Asp Gly Thr Thr Ile Asp 290 295 300 gta gtt taa 921 Val Val 305 <210> 251 <211> 306 <212> PRT <213> Sphingobacterium sp <400> 251 Met Phe Gly Asp Arg Glu Val Arg Arg Ser Tyr Ser Phe Ser Ser Ser 1 5 10 15 Pro Ala Val Val Glu Pro Leu Ala Ile Thr Val Lys Arg Val Asp Asn 20 25 30 Gly Glu Ile Ser Arg Leu Leu His His Arg Thr Arg Val Gly Asp Leu 35 40 45 Val Asp Val Leu Ala Pro Gln Gly Leu Phe Thr Tyr Glu Pro Asp Pro 50 55 60 Thr Thr Ala Arg Thr Leu Phe Leu Phe Gly Ala Gly Val Gly Val Thr 65 70 75 80 Pro Leu Phe Ser Ile Leu Lys Thr Ala Leu Ser Thr Glu Pro Lys Thr 85 90 95 Lys Val Val Leu Ile Tyr Ser Asn Ser Ser Pro Asp Arg Thr Val Phe 100 105 110 Lys Val Glu Leu Glu His Trp Gln Gln Leu Tyr Ala Asp Arg Leu Glu 115 120 125 Ile Ile Trp Ile Tyr Ser Asn Ser Lys Asn Leu Leu Asn Ala His Leu 130 135 140 Asn Arg Glu Asn Leu Leu Arg Phe Val Asn Glu Arg Met Pro Glu Asp 145 150 155 160 Asn Asn Ala Ile Phe Phe Thr Cys Gly Pro Val Phe Tyr Met Asp Leu 165 170 175 Val Arg Phe Thr Leu Leu Gly Leu Gly Ile Pro Asp Glu Asp Ile Arg 180 185 190 Lys Glu Thr Phe His Phe Pro Glu Glu Glu Asp Asp Glu Asp Glu Lys 195 200 205 Glu Asp Asp Pro Val Asp Thr Thr Ala Tyr Asn Ile Leu Leu Arg Phe 210 215 220 Gln Gly Gln Glu Tyr Pro Leu Thr Ile Pro Tyr Asn Lys Thr Ile Leu 225 230 235 240 Gln Ala Gly Leu Asp Asn Asn Ile Lys Leu Pro Tyr Ser Cys Lys Ser 245 250 255 Gly Met Cys Ser Thr Cys Ile Ser Gln Cys Ser Ser Gly Ser Val Arg 260 265 270 Met Asp Tyr Asn Glu Val Leu Thr Asp Arg Glu Val Glu Asn Gly Arg 275 280 285 Cys Leu Ile Cys Thr Ser His Pro Leu Glu Asp Gly Thr Thr Ile Asp 290 295 300 Val Val 305 <210> 252 <211> 3525 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(3525) <223> ydbK gene from E. coli encoding pyruvate-flavodoxin/ferredoxin oxidoreductase <400> 252 atg att act att gac ggt aat ggc gcg gtt gct tcg gtc gca ttt cgc 48 Met Ile Thr Ile Asp Gly Asn Gly Ala Val Ala Ser Val Ala Phe Arg 1 5 10 15 acc agt gaa gtt atc gcc atc tac cct att acc ccc agt tcc acg atg 96 Thr Ser Glu Val Ile Ala Ile Tyr Pro Ile Thr Pro Ser Ser Thr Met 20 25 30 gca gaa cag gct gat gcc tgg gcc gga aac ggc tta aag aac gtt tgg 144 Ala Glu Gln Ala Asp Ala Trp Ala Gly Asn Gly Leu Lys Asn Val Trp 35 40 45 gga gac aca cca cgc gtg gtt gaa atg cag tcg gaa gcg ggt gct atc 192 Gly Asp Thr Pro Arg Val Val Glu Met Gln Ser Glu Ala Gly Ala Ile 50 55 60 gct acc gtg cat ggc gct ttg cag acg ggt gcc ctt tca aca tcg ttt 240 Ala Thr Val His Gly Ala Leu Gln Thr Gly Ala Leu Ser Thr Ser Phe 65 70 75 80 acg tca tcg cag ggt ttg ctg ctg atg atc ccg acg ctg tac aaa ctg 288 Thr Ser Ser Gln Gly Leu Leu Leu Met Ile Pro Thr Leu Tyr Lys Leu 85 90 95 gca ggc gaa cta aca ccg ttt gtc ctg cat gta gcg gca cgt acc gtt 336 Ala Gly Glu Leu Thr Pro Phe Val Leu His Val Ala Ala Arg Thr Val 100 105 110 gcc aca cat gca ctc tct att ttt ggc gat cat tcc gac gtt atg gcg 384 Ala Thr His Ala Leu Ser Ile Phe Gly Asp His Ser Asp Val Met Ala 115 120 125 gtg cgc cag acg ggt tgc gcg atg ttg tgt gca gca aac gtc cag gaa 432 Val Arg Gln Thr Gly Cys Ala Met Leu Cys Ala Ala Asn Val Gln Glu 130 135 140 gcg caa gac ttt gct ctc att tcg caa atc gcg acg ctg aaa agc cgc 480 Ala Gln Asp Phe Ala Leu Ile Ser Gln Ile Ala Thr Leu Lys Ser Arg 145 150 155 160 gtg cca ttt att cat ttc ttt gat ggt ttc cgc acg tcc cac gaa atc 528 Val Pro Phe Ile His Phe Phe Asp Gly Phe Arg Thr Ser His Glu Ile 165 170 175 aat aaa att gtc ccg ctg gcc gat gac acg att ctt gat ctc atg ccg 576 Asn Lys Ile Val Pro Leu Ala Asp Asp Thr Ile Leu Asp Leu Met Pro 180 185 190 cag gtc gaa att gat gct cat cgc gcc cgg gca ctc aac ccg gaa cat 624 Gln Val Glu Ile Asp Ala His Arg Ala Arg Ala Leu Asn Pro Glu His 195 200 205 ccg gtg atc cgc ggt acg tcc gcc aat cct gac act tat ttc cag tct 672 Pro Val Ile Arg Gly Thr Ser Ala Asn Pro Asp Thr Tyr Phe Gln Ser 210 215 220 cgc gaa gcc acc aac cca tgg tac aac gcg gtc tat gac cat gtt gaa 720 Arg Glu Ala Thr Asn Pro Trp Tyr Asn Ala Val Tyr Asp His Val Glu 225 230 235 240 cag gcg atg aat gat ttc tct gcc gcg aca ggt cgt cag tat cag ccg 768 Gln Ala Met Asn Asp Phe Ser Ala Ala Thr Gly Arg Gln Tyr Gln Pro 245 250 255 ttt gaa tat tac ggg cat ccg caa gcg gaa cgg gtg att atc ctg atg 816 Phe Glu Tyr Tyr Gly His Pro Gln Ala Glu Arg Val Ile Ile Leu Met 260 265 270 ggc tct gcc att ggc acc tgt gaa gaa gtg gtt gat gaa ttg cta acc 864 Gly Ser Ala Ile Gly Thr Cys Glu Glu Val Val Asp Glu Leu Leu Thr 275 280 285 cgt ggc gaa aaa gtt ggc gtg ctg aaa gtt cgc ctg tac cgc ccc ttc 912 Arg Gly Glu Lys Val Gly Val Leu Lys Val Arg Leu Tyr Arg Pro Phe 290 295 300 tcc gct aaa cat tta ctg caa gct ctg ccg gga tcc gta cgc agc gtg 960 Ser Ala Lys His Leu Leu Gln Ala Leu Pro Gly Ser Val Arg Ser Val 305 310 315 320 gcg gta ctg gac aga acc aaa gaa ccc ggt gcc cag gca gaa ccg ctc 1008 Ala Val Leu Asp Arg Thr Lys Glu Pro Gly Ala Gln Ala Glu Pro Leu 325 330 335 tat ctg gat gta atg acc gca ctg gca gaa gcc ttt aat aat ggc gag 1056 Tyr Leu Asp Val Met Thr Ala Leu Ala Glu Ala Phe Asn Asn Gly Glu 340 345 350 cgc gaa act ctg ccc cgt gtc att ggt ggg cgc tat ggt ctt tca tcc 1104 Arg Glu Thr Leu Pro Arg Val Ile Gly Gly Arg Tyr Gly Leu Ser Ser 355 360 365 aaa gaa ttt ggc cca gac tgt gta ctg gcg gta ttt gcc gag ctc aac 1152 Lys Glu Phe Gly Pro Asp Cys Val Leu Ala Val Phe Ala Glu Leu Asn 370 375 380 gcg gct aaa ccg aaa gcg cgc ttt acg gtt ggt att tac gat gat gtg 1200 Ala Ala Lys Pro Lys Ala Arg Phe Thr Val Gly Ile Tyr Asp Asp Val 385 390 395 400 acc aat ctg tca ctg ccg ttg ccg gaa aac acc ctg cca aac tcg gcg 1248 Thr Asn Leu Ser Leu Pro Leu Pro Glu Asn Thr Leu Pro Asn Ser Ala 405 410 415 aaa ctg gaa gcc ttg ttt tat ggc ctt ggt agt gat ggc agc gtt tcc 1296 Lys Leu Glu Ala Leu Phe Tyr Gly Leu Gly Ser Asp Gly Ser Val Ser 420 425 430 gcg acc aaa aac aat atc aag att atc ggt aat tcc acg ccg tgg tac 1344 Ala Thr Lys Asn Asn Ile Lys Ile Ile Gly Asn Ser Thr Pro Trp Tyr 435 440 445 gca cag ggc tat ttt gtt tac gac tcc aaa aag gcg ggc ggc ctg acg 1392 Ala Gln Gly Tyr Phe Val Tyr Asp Ser Lys Lys Ala Gly Gly Leu Thr 450 455 460 gtt tct cac ctt cga gtg agc gaa cag ccg att cgt tcc gct tat ctc 1440 Val Ser His Leu Arg Val Ser Glu Gln Pro Ile Arg Ser Ala Tyr Leu 465 470 475 480 att tcc cag gct gat ttt gtt ggc tgc cac cag ttg cag ttt atc gat 1488 Ile Ser Gln Ala Asp Phe Val Gly Cys His Gln Leu Gln Phe Ile Asp 485 490 495 aaa tat cag atg gct gag cgt tta aaa cct ggc ggc att ttc ctg ctc 1536 Lys Tyr Gln Met Ala Glu Arg Leu Lys Pro Gly Gly Ile Phe Leu Leu 500 505 510 aac acg ccg tac agc gca gat gaa gtg tgg tcg cgc ttg ccg caa gaa 1584 Asn Thr Pro Tyr Ser Ala Asp Glu Val Trp Ser Arg Leu Pro Gln Glu 515 520 525 gtt cag gcc gtg tta aac cag aaa aaa gcg cgc ttc tat gtg att aac 1632 Val Gln Ala Val Leu Asn Gln Lys Lys Ala Arg Phe Tyr Val Ile Asn 530 535 540 gcg gcg aaa atc gcc cgc gaa tgt ggc ctg gcg gcc cgt att aat acc 1680 Ala Ala Lys Ile Ala Arg Glu Cys Gly Leu Ala Ala Arg Ile Asn Thr 545 550 555 560 gtc atg cag atg gct ttt ttc cat ctg acg caa att ctg cct ggc gat 1728 Val Met Gln Met Ala Phe Phe His Leu Thr Gln Ile Leu Pro Gly Asp 565 570 575 agc gcc ctc gca gaa ttg cag ggt gcg att gcc aaa agt tac agt agc 1776 Ser Ala Leu Ala Glu Leu Gln Gly Ala Ile Ala Lys Ser Tyr Ser Ser 580 585 590 aaa ggc cag gat ctg gtg gaa cgc aac tgg cag gct ctg gcg ctg gcg 1824 Lys Gly Gln Asp Leu Val Glu Arg Asn Trp Gln Ala Leu Ala Leu Ala 595 600 605 cgt gaa tcc gta gaa gaa gtt ccg ttg caa ccg gta aat ccg cac agc 1872 Arg Glu Ser Val Glu Glu Val Pro Leu Gln Pro Val Asn Pro His Ser 610 615 620 gcc aat cga ccg cca gtg gtt tcc gat gcc gcc cct gat ttc gtg aaa 1920 Ala Asn Arg Pro Pro Val Val Ser Asp Ala Ala Pro Asp Phe Val Lys 625 630 635 640 acc gta acc gct gcg atg ctc gcc ggg ctt ggt gac gcc ctc ccc gtt 1968 Thr Val Thr Ala Ala Met Leu Ala Gly Leu Gly Asp Ala Leu Pro Val 645 650 655 tcg gcg ctg ccg cca gac ggc acc tgg ccg atg ggc act acg cgc tgg 2016 Ser Ala Leu Pro Pro Asp Gly Thr Trp Pro Met Gly Thr Thr Arg Trp 660 665 670 gaa aaa cgc aat atc gcc gaa gag atc ccc atc tgg aaa gag gaa ctc 2064 Glu Lys Arg Asn Ile Ala Glu Glu Ile Pro Ile Trp Lys Glu Glu Leu 675 680 685 tgt acc caa tgt aac cac tgc gtt gcc gct tgc cca cac tca gct att 2112 Cys Thr Gln Cys Asn His Cys Val Ala Ala Cys Pro His Ser Ala Ile 690 695 700 cgc gca aaa gtg gtg ccg cct gaa gcg atg gaa aac gcc cct gcc agc 2160 Arg Ala Lys Val Val Pro Pro Glu Ala Met Glu Asn Ala Pro Ala Ser 705 710 715 720 ctg cat tcg ctg gat gtg aaa tcg cgt gat atg cgc ggg cag aaa tat 2208 Leu His Ser Leu Asp Val Lys Ser Arg Asp Met Arg Gly Gln Lys Tyr 725 730 735 gtc ttg cag gtg gca ccg gaa gat tgc acc ggt tgt aac ctg tgc gtc 2256 Val Leu Gln Val Ala Pro Glu Asp Cys Thr Gly Cys Asn Leu Cys Val 740 745 750 gaa gtt tgc ccg gcg aaa gac cgt cag aat cca gag att aaa gcc atc 2304 Glu Val Cys Pro Ala Lys Asp Arg Gln Asn Pro Glu Ile Lys Ala Ile 755 760 765 aat atg atg tct cgc ctg gaa cat gtc gaa gaa gag aaa atc aat tac 2352 Asn Met Met Ser Arg Leu Glu His Val Glu Glu Glu Lys Ile Asn Tyr 770 775 780 gat ttc ttc ctc aac ctg cca gaa atc gac cgt agc aaa ctg gaa cgt 2400 Asp Phe Phe Leu Asn Leu Pro Glu Ile Asp Arg Ser Lys Leu Glu Arg 785 790 795 800 att gat att cgt aca tcg cag ctg att aca ccg ctg ttt gaa tat tca 2448 Ile Asp Ile Arg Thr Ser Gln Leu Ile Thr Pro Leu Phe Glu Tyr Ser 805 810 815 ggt gct tgc tcc ggt tgt ggc gag acg ccg tat att aaa tta ctg act 2496 Gly Ala Cys Ser Gly Cys Gly Glu Thr Pro Tyr Ile Lys Leu Leu Thr 820 825 830 cag ctc tat ggc gac cgg atg ttg atc gct aac gcc act ggc tgt tct 2544 Gln Leu Tyr Gly Asp Arg Met Leu Ile Ala Asn Ala Thr Gly Cys Ser 835 840 845 tca att tat ggc ggt aac ctg ccc tct aca ccg tat acc acc gat gcc 2592 Ser Ile Tyr Gly Gly Asn Leu Pro Ser Thr Pro Tyr Thr Thr Asp Ala 850 855 860 aac ggt cgt ggg ccg gca tgg gcg aac tct cta ttt gaa gat aat gcc 2640 Asn Gly Arg Gly Pro Ala Trp Ala Asn Ser Leu Phe Glu Asp Asn Ala 865 870 875 880 gaa ttt ggc ctt ggt ttc cgc ctg acg gtc gat caa cac cgt gtc cgc 2688 Glu Phe Gly Leu Gly Phe Arg Leu Thr Val Asp Gln His Arg Val Arg 885 890 895 gtg ctg cgt ctg ctg gat caa ttt gcc gat aaa atc ccg gcg gaa tta 2736 Val Leu Arg Leu Leu Asp Gln Phe Ala Asp Lys Ile Pro Ala Glu Leu 900 905 910 ctg acg gcg ttg aaa tca gac gcc acg cca gag gtt cgt cgt gaa cag 2784 Leu Thr Ala Leu Lys Ser Asp Ala Thr Pro Glu Val Arg Arg Glu Gln 915 920 925 gtt gca gct tta cgc cag caa ctc aac gat gtt gcc gaa gca cat gaa 2832 Val Ala Ala Leu Arg Gln Gln Leu Asn Asp Val Ala Glu Ala His Glu 930 935 940 ctg cta cgt gat gca gat gca ctg gtg gaa aaa tca atc tgg ctg att 2880 Leu Leu Arg Asp Ala Asp Ala Leu Val Glu Lys Ser Ile Trp Leu Ile 945 950 955 960 ggt ggt gat ggc tgg gct tac gat atc ggc ttt ggc ggt ctg gat cat 2928 Gly Gly Asp Gly Trp Ala Tyr Asp Ile Gly Phe Gly Gly Leu Asp His 965 970 975 gta ttg agt ttg acg gaa aac gtc aac att ctg gtg ctg gat acg caa 2976 Val Leu Ser Leu Thr Glu Asn Val Asn Ile Leu Val Leu Asp Thr Gln 980 985 990 tgc tat tcc aac acc ggt ggt cag gcg tcg aaa gcg aca ccg ctg ggt 3024 Cys Tyr Ser Asn Thr Gly Gly Gln Ala Ser Lys Ala Thr Pro Leu Gly 995 1000 1005 gca gta act aaa ttt ggc gag cac ggc aaa cgt aaa gcg cgt aaa 3069 Ala Val Thr Lys Phe Gly Glu His Gly Lys Arg Lys Ala Arg Lys 1010 1015 1020 gat ctt ggc gtc agt atg atg atg tac ggt cat gtt tat gtg gcg 3114 Asp Leu Gly Val Ser Met Met Met Tyr Gly His Val Tyr Val Ala 1025 1030 1035 cag att tct ctc ggc gcg cag ctg aac cag acg gtg aaa gcg att 3159 Gln Ile Ser Leu Gly Ala Gln Leu Asn Gln Thr Val Lys Ala Ile 1040 1045 1050 cag gaa gcg gaa gcg tat ccg ggg cca tcg ctg atc att gct tat 3204 Gln Glu Ala Glu Ala Tyr Pro Gly Pro Ser Leu Ile Ile Ala Tyr 1055 1060 1065 agc ccg tgt gaa gag cat ggt tac gat ctg gca ctc agc cac gac 3249 Ser Pro Cys Glu Glu His Gly Tyr Asp Leu Ala Leu Ser His Asp 1070 1075 1080 cag atg cgc caa ctc aca gct acc ggc ttc tgg ccg cta tat cgc 3294 Gln Met Arg Gln Leu Thr Ala Thr Gly Phe Trp Pro Leu Tyr Arg 1085 1090 1095 ttt gat ccg cgt cgt gcc gat gaa ggc aaa ctg ccg ctg gcc ttg 3339 Phe Asp Pro Arg Arg Ala Asp Glu Gly Lys Leu Pro Leu Ala Leu 1100 1105 1110 gat tca cgc ccg ccg tca gaa gca ccg gaa gaa acg tta ctt cac 3384 Asp Ser Arg Pro Pro Ser Glu Ala Pro Glu Glu Thr Leu Leu His 1115 1120 1125 gag caa cgt ttc cgt cgg ctg aat tcg cag cag cca gaa gtg gca 3429 Glu Gln Arg Phe Arg Arg Leu Asn Ser Gln Gln Pro Glu Val Ala 1130 1135 1140 gaa cag tta tgg aaa gat gct gca gct gat ttg caa aaa cgc tat 3474 Glu Gln Leu Trp Lys Asp Ala Ala Ala Asp Leu Gln Lys Arg Tyr 1145 1150 1155 gac ttc ctg gca caa atg gcc gga aaa gcg gaa aaa agc aac acc 3519 Asp Phe Leu Ala Gln Met Ala Gly Lys Ala Glu Lys Ser Asn Thr 1160 1165 1170 gat taa 3525 Asp <210> 253 <211> 1174 <212> PRT <213> Escherichia coli <400> 253 Met Ile Thr Ile Asp Gly Asn Gly Ala Val Ala Ser Val Ala Phe Arg 1 5 10 15 Thr Ser Glu Val Ile Ala Ile Tyr Pro Ile Thr Pro Ser Ser Thr Met 20 25 30 Ala Glu Gln Ala Asp Ala Trp Ala Gly Asn Gly Leu Lys Asn Val Trp 35 40 45 Gly Asp Thr Pro Arg Val Val Glu Met Gln Ser Glu Ala Gly Ala Ile 50 55 60 Ala Thr Val His Gly Ala Leu Gln Thr Gly Ala Leu Ser Thr Ser Phe 65 70 75 80 Thr Ser Ser Gln Gly Leu Leu Leu Met Ile Pro Thr Leu Tyr Lys Leu 85 90 95 Ala Gly Glu Leu Thr Pro Phe Val Leu His Val Ala Ala Arg Thr Val 100 105 110 Ala Thr His Ala Leu Ser Ile Phe Gly Asp His Ser Asp Val Met Ala 115 120 125 Val Arg Gln Thr Gly Cys Ala Met Leu Cys Ala Ala Asn Val Gln Glu 130 135 140 Ala Gln Asp Phe Ala Leu Ile Ser Gln Ile Ala Thr Leu Lys Ser Arg 145 150 155 160 Val Pro Phe Ile His Phe Phe Asp Gly Phe Arg Thr Ser His Glu Ile 165 170 175 Asn Lys Ile Val Pro Leu Ala Asp Asp Thr Ile Leu Asp Leu Met Pro 180 185 190 Gln Val Glu Ile Asp Ala His Arg Ala Arg Ala Leu Asn Pro Glu His 195 200 205 Pro Val Ile Arg Gly Thr Ser Ala Asn Pro Asp Thr Tyr Phe Gln Ser 210 215 220 Arg Glu Ala Thr Asn Pro Trp Tyr Asn Ala Val Tyr Asp His Val Glu 225 230 235 240 Gln Ala Met Asn Asp Phe Ser Ala Ala Thr Gly Arg Gln Tyr Gln Pro 245 250 255 Phe Glu Tyr Tyr Gly His Pro Gln Ala Glu Arg Val Ile Ile Leu Met 260 265 270 Gly Ser Ala Ile Gly Thr Cys Glu Glu Val Val Asp Glu Leu Leu Thr 275 280 285 Arg Gly Glu Lys Val Gly Val Leu Lys Val Arg Leu Tyr Arg Pro Phe 290 295 300 Ser Ala Lys His Leu Leu Gln Ala Leu Pro Gly Ser Val Arg Ser Val 305 310 315 320 Ala Val Leu Asp Arg Thr Lys Glu Pro Gly Ala Gln Ala Glu Pro Leu 325 330 335 Tyr Leu Asp Val Met Thr Ala Leu Ala Glu Ala Phe Asn Asn Gly Glu 340 345 350 Arg Glu Thr Leu Pro Arg Val Ile Gly Gly Arg Tyr Gly Leu Ser Ser 355 360 365 Lys Glu Phe Gly Pro Asp Cys Val Leu Ala Val Phe Ala Glu Leu Asn 370 375 380 Ala Ala Lys Pro Lys Ala Arg Phe Thr Val Gly Ile Tyr Asp Asp Val 385 390 395 400 Thr Asn Leu Ser Leu Pro Leu Pro Glu Asn Thr Leu Pro Asn Ser Ala 405 410 415 Lys Leu Glu Ala Leu Phe Tyr Gly Leu Gly Ser Asp Gly Ser Val Ser 420 425 430 Ala Thr Lys Asn Asn Ile Lys Ile Ile Gly Asn Ser Thr Pro Trp Tyr 435 440 445 Ala Gln Gly Tyr Phe Val Tyr Asp Ser Lys Lys Ala Gly Gly Leu Thr 450 455 460 Val Ser His Leu Arg Val Ser Glu Gln Pro Ile Arg Ser Ala Tyr Leu 465 470 475 480 Ile Ser Gln Ala Asp Phe Val Gly Cys His Gln Leu Gln Phe Ile Asp 485 490 495 Lys Tyr Gln Met Ala Glu Arg Leu Lys Pro Gly Gly Ile Phe Leu Leu 500 505 510 Asn Thr Pro Tyr Ser Ala Asp Glu Val Trp Ser Arg Leu Pro Gln Glu 515 520 525 Val Gln Ala Val Leu Asn Gln Lys Lys Ala Arg Phe Tyr Val Ile Asn 530 535 540 Ala Ala Lys Ile Ala Arg Glu Cys Gly Leu Ala Ala Arg Ile Asn Thr 545 550 555 560 Val Met Gln Met Ala Phe Phe His Leu Thr Gln Ile Leu Pro Gly Asp 565 570 575 Ser Ala Leu Ala Glu Leu Gln Gly Ala Ile Ala Lys Ser Tyr Ser Ser 580 585 590 Lys Gly Gln Asp Leu Val Glu Arg Asn Trp Gln Ala Leu Ala Leu Ala 595 600 605 Arg Glu Ser Val Glu Glu Val Pro Leu Gln Pro Val Asn Pro His Ser 610 615 620 Ala Asn Arg Pro Pro Val Val Ser Asp Ala Ala Pro Asp Phe Val Lys 625 630 635 640 Thr Val Thr Ala Ala Met Leu Ala Gly Leu Gly Asp Ala Leu Pro Val 645 650 655 Ser Ala Leu Pro Pro Asp Gly Thr Trp Pro Met Gly Thr Thr Arg Trp 660 665 670 Glu Lys Arg Asn Ile Ala Glu Glu Ile Pro Ile Trp Lys Glu Glu Leu 675 680 685 Cys Thr Gln Cys Asn His Cys Val Ala Ala Cys Pro His Ser Ala Ile 690 695 700 Arg Ala Lys Val Val Pro Pro Glu Ala Met Glu Asn Ala Pro Ala Ser 705 710 715 720 Leu His Ser Leu Asp Val Lys Ser Arg Asp Met Arg Gly Gln Lys Tyr 725 730 735 Val Leu Gln Val Ala Pro Glu Asp Cys Thr Gly Cys Asn Leu Cys Val 740 745 750 Glu Val Cys Pro Ala Lys Asp Arg Gln Asn Pro Glu Ile Lys Ala Ile 755 760 765 Asn Met Met Ser Arg Leu Glu His Val Glu Glu Glu Lys Ile Asn Tyr 770 775 780 Asp Phe Phe Leu Asn Leu Pro Glu Ile Asp Arg Ser Lys Leu Glu Arg 785 790 795 800 Ile Asp Ile Arg Thr Ser Gln Leu Ile Thr Pro Leu Phe Glu Tyr Ser 805 810 815 Gly Ala Cys Ser Gly Cys Gly Glu Thr Pro Tyr Ile Lys Leu Leu Thr 820 825 830 Gln Leu Tyr Gly Asp Arg Met Leu Ile Ala Asn Ala Thr Gly Cys Ser 835 840 845 Ser Ile Tyr Gly Gly Asn Leu Pro Ser Thr Pro Tyr Thr Thr Asp Ala 850 855 860 Asn Gly Arg Gly Pro Ala Trp Ala Asn Ser Leu Phe Glu Asp Asn Ala 865 870 875 880 Glu Phe Gly Leu Gly Phe Arg Leu Thr Val Asp Gln His Arg Val Arg 885 890 895 Val Leu Arg Leu Leu Asp Gln Phe Ala Asp Lys Ile Pro Ala Glu Leu 900 905 910 Leu Thr Ala Leu Lys Ser Asp Ala Thr Pro Glu Val Arg Arg Glu Gln 915 920 925 Val Ala Ala Leu Arg Gln Gln Leu Asn Asp Val Ala Glu Ala His Glu 930 935 940 Leu Leu Arg Asp Ala Asp Ala Leu Val Glu Lys Ser Ile Trp Leu Ile 945 950 955 960 Gly Gly Asp Gly Trp Ala Tyr Asp Ile Gly Phe Gly Gly Leu Asp His 965 970 975 Val Leu Ser Leu Thr Glu Asn Val Asn Ile Leu Val Leu Asp Thr Gln 980 985 990 Cys Tyr Ser Asn Thr Gly Gly Gln Ala Ser Lys Ala Thr Pro Leu Gly 995 1000 1005 Ala Val Thr Lys Phe Gly Glu His Gly Lys Arg Lys Ala Arg Lys 1010 1015 1020 Asp Leu Gly Val Ser Met Met Met Tyr Gly His Val Tyr Val Ala 1025 1030 1035 Gln Ile Ser Leu Gly Ala Gln Leu Asn Gln Thr Val Lys Ala Ile 1040 1045 1050 Gln Glu Ala Glu Ala Tyr Pro Gly Pro Ser Leu Ile Ile Ala Tyr 1055 1060 1065 Ser Pro Cys Glu Glu His Gly Tyr Asp Leu Ala Leu Ser His Asp 1070 1075 1080 Gln Met Arg Gln Leu Thr Ala Thr Gly Phe Trp Pro Leu Tyr Arg 1085 1090 1095 Phe Asp Pro Arg Arg Ala Asp Glu Gly Lys Leu Pro Leu Ala Leu 1100 1105 1110 Asp Ser Arg Pro Pro Ser Glu Ala Pro Glu Glu Thr Leu Leu His 1115 1120 1125 Glu Gln Arg Phe Arg Arg Leu Asn Ser Gln Gln Pro Glu Val Ala 1130 1135 1140 Glu Gln Leu Trp Lys Asp Ala Ala Ala Asp Leu Gln Lys Arg Tyr 1145 1150 1155 Asp Phe Leu Ala Gln Met Ala Gly Lys Ala Glu Lys Ser Asn Thr 1160 1165 1170 Asp <210> 254 <211> 3588 <212> DNA <213> Geobacter sulfurreducens <220> <221> CDS <222> (1)..(3588) <223> por gene from Geobacter sulfurreducens AM-1 encoding pyruvate-flavodoxin/ferredoxin oxidoreductase <400> 254 atg agt cgc aaa atg gta acc atc gac ggc aat acc gcg gct gcc cac 48 Met Ser Arg Lys Met Val Thr Ile Asp Gly Asn Thr Ala Ala Ala His 1 5 10 15 gtg gcg cac gcc acc aac gag gtc att gcc atc tac ccc att acc cct 96 Val Ala His Ala Thr Asn Glu Val Ile Ala Ile Tyr Pro Ile Thr Pro 20 25 30 tcg tcg gtc atg ggt gag att tcc gac atc aag agc gcc atg ggc gag 144 Ser Ser Val Met Gly Glu Ile Ser Asp Ile Lys Ser Ala Met Gly Glu 35 40 45 aaa aac atc tgg gga acc gta ccg tcg gtt gtc gag atg cag tcg gaa 192 Lys Asn Ile Trp Gly Thr Val Pro Ser Val Val Glu Met Gln Ser Glu 50 55 60 ggc ggc gct gcc ggt gcc gtg cac ggt gcc ctc cag gca ggt gcg ctg 240 Gly Gly Ala Ala Gly Ala Val His Gly Ala Leu Gln Ala Gly Ala Leu 65 70 75 80 acc acc act ttt acc gcc agc cag ggt ctg ctc ctg atg atc ccg aac 288 Thr Thr Thr Phe Thr Ala Ser Gln Gly Leu Leu Leu Met Ile Pro Asn 85 90 95 atg ttc aag atc gcc ggc gag ctg acc tct acg gtc ttc cat gtc tcc 336 Met Phe Lys Ile Ala Gly Glu Leu Thr Ser Thr Val Phe His Val Ser 100 105 110 gcc cgc gcc atc gcg gcc cag gcc ctc tcc atc ttt ggc gac cat tcg 384 Ala Arg Ala Ile Ala Ala Gln Ala Leu Ser Ile Phe Gly Asp His Ser 115 120 125 gac gtc atg tcc tgc cgt tcc acc ggt tgg gcc atg ctc tgc tcc aac 432 Asp Val Met Ser Cys Arg Ser Thr Gly Trp Ala Met Leu Cys Ser Asn 130 135 140 aac tcc cag gag gtc atg gac ttc gcc ctg att gcc cag tcc gcg acg 480 Asn Ser Gln Glu Val Met Asp Phe Ala Leu Ile Ala Gln Ser Ala Thr 145 150 155 160 ctt cgt tcc cgg gtg ccg ttc ctc cat ttc ttc gac ggc ttc cgg acc 528 Leu Arg Ser Arg Val Pro Phe Leu His Phe Phe Asp Gly Phe Arg Thr 165 170 175 tcc cac gag gtt ctc aag gtg gag gag ctg act ttc gac gac atg cgc 576 Ser His Glu Val Leu Lys Val Glu Glu Leu Thr Phe Asp Asp Met Arg 180 185 190 gcc atg ctg gac gac gaa ctg atc gcc gcc cac aag gcc cgg ggc ctc 624 Ala Met Leu Asp Asp Glu Leu Ile Ala Ala His Lys Ala Arg Gly Leu 195 200 205 tct ccg gac cat ccc gtc atg cgc ggc acc gcc cag aac cct gac gtc 672 Ser Pro Asp His Pro Val Met Arg Gly Thr Ala Gln Asn Pro Asp Val 210 215 220 tac ttc cag ggg cgc gag acc gtt aac ccc ttc tac ccg aaa tgc atc 720 Tyr Phe Gln Gly Arg Glu Thr Val Asn Pro Phe Tyr Pro Lys Cys Ile 225 230 235 240 gag atc gtg gca gag gag atg gac aag ttc gcc aag atc acg ggc cgc 768 Glu Ile Val Ala Glu Glu Met Asp Lys Phe Ala Lys Ile Thr Gly Arg 245 250 255 cag tac aag ctg gtg gac tac gtg ggc gcc ccc gac gcc gac cgg gtc 816 Gln Tyr Lys Leu Val Asp Tyr Val Gly Ala Pro Asp Ala Asp Arg Val 260 265 270 atc gtc atc atg gga tcc ggc gcc gac acg gtg cag gag acc gtg gag 864 Ile Val Ile Met Gly Ser Gly Ala Asp Thr Val Gln Glu Thr Val Glu 275 280 285 cac ctg aac acc aag ggt gag aag atc ggc gtg gtg aag gtc cac ctc 912 His Leu Asn Thr Lys Gly Glu Lys Ile Gly Val Val Lys Val His Leu 290 295 300 tac cgg ccg ttc ccc atc gat gcc ttc att gcc gcc ctg ccc aag acc 960 Tyr Arg Pro Phe Pro Ile Asp Ala Phe Ile Ala Ala Leu Pro Lys Thr 305 310 315 320 gtg aag aag atc gcg gtc ctc gac cgg acc aag gag ccc ggc gcc ctg 1008 Val Lys Lys Ile Ala Val Leu Asp Arg Thr Lys Glu Pro Gly Ala Leu 325 330 335 ggc gag ccc ctg tac ctg gat gtc cgc act gcc atc ggc gag gcc atg 1056 Gly Glu Pro Leu Tyr Leu Asp Val Arg Thr Ala Ile Gly Glu Ala Met 340 345 350 gcc gac ggg aag tgc cag ttc gac ggc tac ccg gtc atc gtg ggc ggt 1104 Ala Asp Gly Lys Cys Gln Phe Asp Gly Tyr Pro Val Ile Val Gly Gly 355 360 365 cgc tac ggc ctt ggt tcc aag gag ttc acc ccg gcc cag gcc aag gcg 1152 Arg Tyr Gly Leu Gly Ser Lys Glu Phe Thr Pro Ala Gln Ala Lys Ala 370 375 380 gtg ttc gat aac cta gcc act gcc aag ccg cag aac aag ttc gtg gtc 1200 Val Phe Asp Asn Leu Ala Thr Ala Lys Pro Gln Asn Lys Phe Val Val 385 390 395 400 ggc atc acc gag gac gtg acc aac agc agc ctc ccg tgt gat ccg tcc 1248 Gly Ile Thr Glu Asp Val Thr Asn Ser Ser Leu Pro Cys Asp Pro Ser 405 410 415 ttc ttc aac ccg atg gaa ggg gcc tac cag gcc atg ttc ttc ggc ctc 1296 Phe Phe Asn Pro Met Glu Gly Ala Tyr Gln Ala Met Phe Phe Gly Leu 420 425 430 ggc tcc gac ggt acc gtg ggc gcc aac aag aac tcc atc aag atc atc 1344 Gly Ser Asp Gly Thr Val Gly Ala Asn Lys Asn Ser Ile Lys Ile Ile 435 440 445 ggc gag atg acc gat aac aac gcc cag gcc tac ttc gtc tac gac tcc 1392 Gly Glu Met Thr Asp Asn Asn Ala Gln Ala Tyr Phe Val Tyr Asp Ser 450 455 460 aag aag gcc ggc tcc atg acc acc tcg cac ctg cgc ttc ggc aag aag 1440 Lys Lys Ala Gly Ser Met Thr Thr Ser His Leu Arg Phe Gly Lys Lys 465 470 475 480 tac atc aga gcg ccg tac ctg gtg cag gag gcc gac ttc gtg gcc tgc 1488 Tyr Ile Arg Ala Pro Tyr Leu Val Gln Glu Ala Asp Phe Val Ala Cys 485 490 495 cac aac ttc gcc ttc gtg gaa aag tac gac atg ctg gcc aag gcc aag 1536 His Asn Phe Ala Phe Val Glu Lys Tyr Asp Met Leu Ala Lys Ala Lys 500 505 510 cag ggt gcc acg ttc ctc ctg aac gcc cct tac gac cac aac gag gtg 1584 Gln Gly Ala Thr Phe Leu Leu Asn Ala Pro Tyr Asp His Asn Glu Val 515 520 525 tgg gac agg ctc ccc gcc gac atg cag cag cag atc atc gac aag aag 1632 Trp Asp Arg Leu Pro Ala Asp Met Gln Gln Gln Ile Ile Asp Lys Lys 530 535 540 ctc aag ttc ttc gtg atc gat ggg gta cgc ctc ggc aac gag atc ggg 1680 Leu Lys Phe Phe Val Ile Asp Gly Val Arg Leu Gly Asn Glu Ile Gly 545 550 555 560 ctc ggt ccc cgg atc aac gtg atc atg cag acc gcc ttc ttc aag ata 1728 Leu Gly Pro Arg Ile Asn Val Ile Met Gln Thr Ala Phe Phe Lys Ile 565 570 575 tcc aac atc atc ccg ctg gat cag gcc att gac gag atc aag gac gct 1776 Ser Asn Ile Ile Pro Leu Asp Gln Ala Ile Asp Glu Ile Lys Asp Ala 580 585 590 atc aag aaa acc tat ggc aag gca ggc gag aag gtc gtg gag atg aac 1824 Ile Lys Lys Thr Tyr Gly Lys Ala Gly Glu Lys Val Val Glu Met Asn 595 600 605 tac aag gcg gtt gag gcc ggc ctc aac aac ttc tac gag gta acg gta 1872 Tyr Lys Ala Val Glu Ala Gly Leu Asn Asn Phe Tyr Glu Val Thr Val 610 615 620 ccg gca acg gca acc agt acc ctc cag aag cct ccc gtg gtc agc gcc 1920 Pro Ala Thr Ala Thr Ser Thr Leu Gln Lys Pro Pro Val Val Ser Ala 625 630 635 640 agg gcc ccc cag ttc gtg cag gag acc acc gcc ccc atc atc gcc ggc 1968 Arg Ala Pro Gln Phe Val Gln Glu Thr Thr Ala Pro Ile Ile Ala Gly 645 650 655 ctc ggc gac gac ctg ccg gtg tcc aag atg ccg gcc gac ggc acc ttc 2016 Leu Gly Asp Asp Leu Pro Val Ser Lys Met Pro Ala Asp Gly Thr Phe 660 665 670 ccg acg gcg acc tcc cag ttc gaa aag cgg aac atc gcc gtg gag atc 2064 Pro Thr Ala Thr Ser Gln Phe Glu Lys Arg Asn Ile Ala Val Glu Ile 675 680 685 ccc gtg tgg gac gag cag ctc tgc atc cag tgc ggc atc tgc tcc ttc 2112 Pro Val Trp Asp Glu Gln Leu Cys Ile Gln Cys Gly Ile Cys Ser Phe 690 695 700 gtc tgc ccc cac gcc acc atc agg atg aag gcc tat gac gcc tcc gcc 2160 Val Cys Pro His Ala Thr Ile Arg Met Lys Ala Tyr Asp Ala Ser Ala 705 710 715 720 ctt gcc ggc gcc ccg gca gcg ttc aag tcg gtt gac tgc aag att ccc 2208 Leu Ala Gly Ala Pro Ala Ala Phe Lys Ser Val Asp Cys Lys Ile Pro 725 730 735 gag ttc aag ggg cag aag ttc acc atc cag gta gcc ccg gaa gac tgc 2256 Glu Phe Lys Gly Gln Lys Phe Thr Ile Gln Val Ala Pro Glu Asp Cys 740 745 750 acc ggc tgc ggc gcc tgc gtc cac aac tgc ccg gcc aag agt aag gaa 2304 Thr Gly Cys Gly Ala Cys Val His Asn Cys Pro Ala Lys Ser Lys Glu 755 760 765 gac ccg aac cac aag gcc atc aat atg gca tac cag ccg ccc ctg cgt 2352 Asp Pro Asn His Lys Ala Ile Asn Met Ala Tyr Gln Pro Pro Leu Arg 770 775 780 tct caa gag gtc gag aac tgg gac ttc ttc ctc acc atc ccg gac gtg 2400 Ser Gln Glu Val Glu Asn Trp Asp Phe Phe Leu Thr Ile Pro Asp Val 785 790 795 800 gac ccc acc gta gcc aag ctg gac acg gtc cgc ggt tcc cag ttg gtg 2448 Asp Pro Thr Val Ala Lys Leu Asp Thr Val Arg Gly Ser Gln Leu Val 805 810 815 cgg ccg ctg ttc gaa ttc tcc ggc gcc tgc ctc ggc tgc ggc gag acc 2496 Arg Pro Leu Phe Glu Phe Ser Gly Ala Cys Leu Gly Cys Gly Glu Thr 820 825 830 ccg tac ctg aag ctc ctg acc cag ctc ttc ggc gac cgg acc gtc att 2544 Pro Tyr Leu Lys Leu Leu Thr Gln Leu Phe Gly Asp Arg Thr Val Ile 835 840 845 gcc aac gcc acc ggc tgc tcc tcc atc tac ggc gga aac ctg ccc acc 2592 Ala Asn Ala Thr Gly Cys Ser Ser Ile Tyr Gly Gly Asn Leu Pro Thr 850 855 860 acc ccc tat gcc cag cgg gcc gac ggg ctt ggg ccg gca tgg tcg aac 2640 Thr Pro Tyr Ala Gln Arg Ala Asp Gly Leu Gly Pro Ala Trp Ser Asn 865 870 875 880 tcc ctg ttc gag gac aac gcc gag ttc ggc tac ggc atg cgt ctg gcc 2688 Ser Leu Phe Glu Asp Asn Ala Glu Phe Gly Tyr Gly Met Arg Leu Ala 885 890 895 gtg gat aaa ttc aac gcc atg gcc ctt gag ctg gtc gac aag ctt tcg 2736 Val Asp Lys Phe Asn Ala Met Ala Leu Glu Leu Val Asp Lys Leu Ser 900 905 910 tct tcc tgc tcc tgc tct tcc tgc acg agc gcg gtg ccc ctc atg aac 2784 Ser Ser Cys Ser Cys Ser Ser Cys Thr Ser Ala Val Pro Leu Met Asn 915 920 925 gag atc aag ggc gcc gac cag tcg agc cag gcc ggc atc gag gcc cag 2832 Glu Ile Lys Gly Ala Asp Gln Ser Ser Gln Ala Gly Ile Glu Ala Gln 930 935 940 cgg gcc cgg gtg gcg gag ctg aag aag acc ctt gct tcc tgt ccc gag 2880 Arg Ala Arg Val Ala Glu Leu Lys Lys Thr Leu Ala Ser Cys Pro Glu 945 950 955 960 ccg gat gcc aag cgc ctg ctc acc gtg gcc gac tac ctg gtc aag aag 2928 Pro Asp Ala Lys Arg Leu Leu Thr Val Ala Asp Tyr Leu Val Lys Lys 965 970 975 tcc gtc tgg tgc atc ggc ggc gac ggc tgg gcg tac gat atc ggc tac 2976 Ser Val Trp Cys Ile Gly Gly Asp Gly Trp Ala Tyr Asp Ile Gly Tyr 980 985 990 ggc ggc ctc gac cac gtc atc gcc agc ggc aag aac atc aat ctg ctg 3024 Gly Gly Leu Asp His Val Ile Ala Ser Gly Lys Asn Ile Asn Leu Leu 995 1000 1005 gtg ctc gac acc gag gtc tac tcc aac acc ggc ggc cag gct tcc 3069 Val Leu Asp Thr Glu Val Tyr Ser Asn Thr Gly Gly Gln Ala Ser 1010 1015 1020 aag tcg acc ccg ctg ggc gcc gtg gcc cag ttc gcc gcc ggc ggt 3114 Lys Ser Thr Pro Leu Gly Ala Val Ala Gln Phe Ala Ala Gly Gly 1025 1030 1035 aag ccg gtc tcc aag aag gat ctc ggc atg atg gcc atg gcc tac 3159 Lys Pro Val Ser Lys Lys Asp Leu Gly Met Met Ala Met Ala Tyr 1040 1045 1050 ggg tcg gtc tac gtg gcc act gtc tcc ctc gcc aat ccg gcc cag 3204 Gly Ser Val Tyr Val Ala Thr Val Ser Leu Ala Asn Pro Ala Gln 1055 1060 1065 tgc atc aag gcg ttc ctg gag gcc gag gcc tat gac ggt ccg tcg 3249 Cys Ile Lys Ala Phe Leu Glu Ala Glu Ala Tyr Asp Gly Pro Ser 1070 1075 1080 ctc atc atc gcc tat gcc cac tgc atc gcc cac ggc atc gac atg 3294 Leu Ile Ile Ala Tyr Ala His Cys Ile Ala His Gly Ile Asp Met 1085 1090 1095 acc agc ggc gtg gat gcc cag aag cgg gcg gtt cag tcc ggt tac 3339 Thr Ser Gly Val Asp Ala Gln Lys Arg Ala Val Gln Ser Gly Tyr 1100 1105 1110 tgg ccc ctc tac cgc tat aat ccg cag ctg gcc gcc gag tgc aag 3384 Trp Pro Leu Tyr Arg Tyr Asn Pro Gln Leu Ala Ala Glu Cys Lys 1115 1120 1125 aac ccg ctc cag ctc gac agc aag gcc ccg acc atc gcc ttt gaa 3429 Asn Pro Leu Gln Leu Asp Ser Lys Ala Pro Thr Ile Ala Phe Glu 1130 1135 1140 gag tac gtc aac agc gag aac cgc tac cgc gtc ctc aag aag aac 3474 Glu Tyr Val Asn Ser Glu Asn Arg Tyr Arg Val Leu Lys Lys Asn 1145 1150 1155 aac ccg aaa ggg tac gag gat ctc atg aga aaa gcg gcg gca tgg 3519 Asn Pro Lys Gly Tyr Glu Asp Leu Met Arg Lys Ala Ala Ala Trp 1160 1165 1170 tcc aag gcc cac ttc agc tac tac cag aag ctg gcg gcc ctc aac 3564 Ser Lys Ala His Phe Ser Tyr Tyr Gln Lys Leu Ala Ala Leu Asn 1175 1180 1185 ttc gag gat acc tgc gag aag tag 3588 Phe Glu Asp Thr Cys Glu Lys 1190 1195 <210> 255 <211> 1195 <212> PRT <213> Geobacter sulfurreducens <400> 255 Met Ser Arg Lys Met Val Thr Ile Asp Gly Asn Thr Ala Ala Ala His 1 5 10 15 Val Ala His Ala Thr Asn Glu Val Ile Ala Ile Tyr Pro Ile Thr Pro 20 25 30 Ser Ser Val Met Gly Glu Ile Ser Asp Ile Lys Ser Ala Met Gly Glu 35 40 45 Lys Asn Ile Trp Gly Thr Val Pro Ser Val Val Glu Met Gln Ser Glu 50 55 60 Gly Gly Ala Ala Gly Ala Val His Gly Ala Leu Gln Ala Gly Ala Leu 65 70 75 80 Thr Thr Thr Phe Thr Ala Ser Gln Gly Leu Leu Leu Met Ile Pro Asn 85 90 95 Met Phe Lys Ile Ala Gly Glu Leu Thr Ser Thr Val Phe His Val Ser 100 105 110 Ala Arg Ala Ile Ala Ala Gln Ala Leu Ser Ile Phe Gly Asp His Ser 115 120 125 Asp Val Met Ser Cys Arg Ser Thr Gly Trp Ala Met Leu Cys Ser Asn 130 135 140 Asn Ser Gln Glu Val Met Asp Phe Ala Leu Ile Ala Gln Ser Ala Thr 145 150 155 160 Leu Arg Ser Arg Val Pro Phe Leu His Phe Phe Asp Gly Phe Arg Thr 165 170 175 Ser His Glu Val Leu Lys Val Glu Glu Leu Thr Phe Asp Asp Met Arg 180 185 190 Ala Met Leu Asp Asp Glu Leu Ile Ala Ala His Lys Ala Arg Gly Leu 195 200 205 Ser Pro Asp His Pro Val Met Arg Gly Thr Ala Gln Asn Pro Asp Val 210 215 220 Tyr Phe Gln Gly Arg Glu Thr Val Asn Pro Phe Tyr Pro Lys Cys Ile 225 230 235 240 Glu Ile Val Ala Glu Glu Met Asp Lys Phe Ala Lys Ile Thr Gly Arg 245 250 255 Gln Tyr Lys Leu Val Asp Tyr Val Gly Ala Pro Asp Ala Asp Arg Val 260 265 270 Ile Val Ile Met Gly Ser Gly Ala Asp Thr Val Gln Glu Thr Val Glu 275 280 285 His Leu Asn Thr Lys Gly Glu Lys Ile Gly Val Val Lys Val His Leu 290 295 300 Tyr Arg Pro Phe Pro Ile Asp Ala Phe Ile Ala Ala Leu Pro Lys Thr 305 310 315 320 Val Lys Lys Ile Ala Val Leu Asp Arg Thr Lys Glu Pro Gly Ala Leu 325 330 335 Gly Glu Pro Leu Tyr Leu Asp Val Arg Thr Ala Ile Gly Glu Ala Met 340 345 350 Ala Asp Gly Lys Cys Gln Phe Asp Gly Tyr Pro Val Ile Val Gly Gly 355 360 365 Arg Tyr Gly Leu Gly Ser Lys Glu Phe Thr Pro Ala Gln Ala Lys Ala 370 375 380 Val Phe Asp Asn Leu Ala Thr Ala Lys Pro Gln Asn Lys Phe Val Val 385 390 395 400 Gly Ile Thr Glu Asp Val Thr Asn Ser Ser Leu Pro Cys Asp Pro Ser 405 410 415 Phe Phe Asn Pro Met Glu Gly Ala Tyr Gln Ala Met Phe Phe Gly Leu 420 425 430 Gly Ser Asp Gly Thr Val Gly Ala Asn Lys Asn Ser Ile Lys Ile Ile 435 440 445 Gly Glu Met Thr Asp Asn Asn Ala Gln Ala Tyr Phe Val Tyr Asp Ser 450 455 460 Lys Lys Ala Gly Ser Met Thr Thr Ser His Leu Arg Phe Gly Lys Lys 465 470 475 480 Tyr Ile Arg Ala Pro Tyr Leu Val Gln Glu Ala Asp Phe Val Ala Cys 485 490 495 His Asn Phe Ala Phe Val Glu Lys Tyr Asp Met Leu Ala Lys Ala Lys 500 505 510 Gln Gly Ala Thr Phe Leu Leu Asn Ala Pro Tyr Asp His Asn Glu Val 515 520 525 Trp Asp Arg Leu Pro Ala Asp Met Gln Gln Gln Ile Ile Asp Lys Lys 530 535 540 Leu Lys Phe Phe Val Ile Asp Gly Val Arg Leu Gly Asn Glu Ile Gly 545 550 555 560 Leu Gly Pro Arg Ile Asn Val Ile Met Gln Thr Ala Phe Phe Lys Ile 565 570 575 Ser Asn Ile Ile Pro Leu Asp Gln Ala Ile Asp Glu Ile Lys Asp Ala 580 585 590 Ile Lys Lys Thr Tyr Gly Lys Ala Gly Glu Lys Val Val Glu Met Asn 595 600 605 Tyr Lys Ala Val Glu Ala Gly Leu Asn Asn Phe Tyr Glu Val Thr Val 610 615 620 Pro Ala Thr Ala Thr Ser Thr Leu Gln Lys Pro Pro Val Val Ser Ala 625 630 635 640 Arg Ala Pro Gln Phe Val Gln Glu Thr Thr Ala Pro Ile Ile Ala Gly 645 650 655 Leu Gly Asp Asp Leu Pro Val Ser Lys Met Pro Ala Asp Gly Thr Phe 660 665 670 Pro Thr Ala Thr Ser Gln Phe Glu Lys Arg Asn Ile Ala Val Glu Ile 675 680 685 Pro Val Trp Asp Glu Gln Leu Cys Ile Gln Cys Gly Ile Cys Ser Phe 690 695 700 Val Cys Pro His Ala Thr Ile Arg Met Lys Ala Tyr Asp Ala Ser Ala 705 710 715 720 Leu Ala Gly Ala Pro Ala Ala Phe Lys Ser Val Asp Cys Lys Ile Pro 725 730 735 Glu Phe Lys Gly Gln Lys Phe Thr Ile Gln Val Ala Pro Glu Asp Cys 740 745 750 Thr Gly Cys Gly Ala Cys Val His Asn Cys Pro Ala Lys Ser Lys Glu 755 760 765 Asp Pro Asn His Lys Ala Ile Asn Met Ala Tyr Gln Pro Pro Leu Arg 770 775 780 Ser Gln Glu Val Glu Asn Trp Asp Phe Phe Leu Thr Ile Pro Asp Val 785 790 795 800 Asp Pro Thr Val Ala Lys Leu Asp Thr Val Arg Gly Ser Gln Leu Val 805 810 815 Arg Pro Leu Phe Glu Phe Ser Gly Ala Cys Leu Gly Cys Gly Glu Thr 820 825 830 Pro Tyr Leu Lys Leu Leu Thr Gln Leu Phe Gly Asp Arg Thr Val Ile 835 840 845 Ala Asn Ala Thr Gly Cys Ser Ser Ile Tyr Gly Gly Asn Leu Pro Thr 850 855 860 Thr Pro Tyr Ala Gln Arg Ala Asp Gly Leu Gly Pro Ala Trp Ser Asn 865 870 875 880 Ser Leu Phe Glu Asp Asn Ala Glu Phe Gly Tyr Gly Met Arg Leu Ala 885 890 895 Val Asp Lys Phe Asn Ala Met Ala Leu Glu Leu Val Asp Lys Leu Ser 900 905 910 Ser Ser Cys Ser Cys Ser Ser Cys Thr Ser Ala Val Pro Leu Met Asn 915 920 925 Glu Ile Lys Gly Ala Asp Gln Ser Ser Gln Ala Gly Ile Glu Ala Gln 930 935 940 Arg Ala Arg Val Ala Glu Leu Lys Lys Thr Leu Ala Ser Cys Pro Glu 945 950 955 960 Pro Asp Ala Lys Arg Leu Leu Thr Val Ala Asp Tyr Leu Val Lys Lys 965 970 975 Ser Val Trp Cys Ile Gly Gly Asp Gly Trp Ala Tyr Asp Ile Gly Tyr 980 985 990 Gly Gly Leu Asp His Val Ile Ala Ser Gly Lys Asn Ile Asn Leu Leu 995 1000 1005 Val Leu Asp Thr Glu Val Tyr Ser Asn Thr Gly Gly Gln Ala Ser 1010 1015 1020 Lys Ser Thr Pro Leu Gly Ala Val Ala Gln Phe Ala Ala Gly Gly 1025 1030 1035 Lys Pro Val Ser Lys Lys Asp Leu Gly Met Met Ala Met Ala Tyr 1040 1045 1050 Gly Ser Val Tyr Val Ala Thr Val Ser Leu Ala Asn Pro Ala Gln 1055 1060 1065 Cys Ile Lys Ala Phe Leu Glu Ala Glu Ala Tyr Asp Gly Pro Ser 1070 1075 1080 Leu Ile Ile Ala Tyr Ala His Cys Ile Ala His Gly Ile Asp Met 1085 1090 1095 Thr Ser Gly Val Asp Ala Gln Lys Arg Ala Val Gln Ser Gly Tyr 1100 1105 1110 Trp Pro Leu Tyr Arg Tyr Asn Pro Gln Leu Ala Ala Glu Cys Lys 1115 1120 1125 Asn Pro Leu Gln Leu Asp Ser Lys Ala Pro Thr Ile Ala Phe Glu 1130 1135 1140 Glu Tyr Val Asn Ser Glu Asn Arg Tyr Arg Val Leu Lys Lys Asn 1145 1150 1155 Asn Pro Lys Gly Tyr Glu Asp Leu Met Arg Lys Ala Ala Ala Trp 1160 1165 1170 Ser Lys Ala His Phe Ser Tyr Tyr Gln Lys Leu Ala Ala Leu Asn 1175 1180 1185 Phe Glu Asp Thr Cys Glu Lys 1190 1195 <210> 256 <211> 1956 <212> DNA <213> Streptomyces pratensis <220> <221> CDS <222> (1)..(1956) <223> Sfla_2592 gene from Streptomyces pratensis ATCC 33331encoding pyruvate-flavodoxin/ferredoxin oxidoreductase <400> 256 atg acc agc cag gtc agt agc cca gcc gga aag tcc gat gag gcc agc 48 Met Thr Ser Gln Val Ser Ser Pro Ala Gly Lys Ser Asp Glu Ala Ser 1 5 10 15 gag gct gtc gtc ggg gaa cag cgc gcc ccg cac atc gcc ggt gcg ggt 96 Glu Ala Val Val Gly Glu Gln Arg Ala Pro His Ile Ala Gly Ala Gly 20 25 30 ggc acg gag aag gaa atc cgc cgt ctg gac cgg gtg atc atc cgt ttc 144 Gly Thr Glu Lys Glu Ile Arg Arg Leu Asp Arg Val Ile Ile Arg Phe 35 40 45 gcg ggt gac tcg ggt gac ggt atg cag ttg acg ggc gac cgt ttc acg 192 Ala Gly Asp Ser Gly Asp Gly Met Gln Leu Thr Gly Asp Arg Phe Thr 50 55 60 tcg gag acg gcg tcg ttc ggg aac gac ctg tcg aca ctg ccc aac ttc 240 Ser Glu Thr Ala Ser Phe Gly Asn Asp Leu Ser Thr Leu Pro Asn Phe 65 70 75 80 ccg gcc gag atc cgg gca ccc gcc ggc acc ctg ccc ggg gtg tcg tcg 288 Pro Ala Glu Ile Arg Ala Pro Ala Gly Thr Leu Pro Gly Val Ser Ser 85 90 95 ttc cag ctg cat ttc gcg gac cac gac atc ctg aca ccg ggc gac gcg 336 Phe Gln Leu His Phe Ala Asp His Asp Ile Leu Thr Pro Gly Asp Ala 100 105 110 ccg aac gtc ctg gtc gcg atg aat ccc gcc gcg ctg aag gcg aat atc 384 Pro Asn Val Leu Val Ala Met Asn Pro Ala Ala Leu Lys Ala Asn Ile 115 120 125 gcc gat gtg ccg cgc ggg gcc gac atc atc gtg aac acg gac gag ttc 432 Ala Asp Val Pro Arg Gly Ala Asp Ile Ile Val Asn Thr Asp Glu Phe 130 135 140 acg aag cgc ccg atg gcg aaa gtc gga tat gcg gaa tcc cct ttg gag 480 Thr Lys Arg Pro Met Ala Lys Val Gly Tyr Ala Glu Ser Pro Leu Glu 145 150 155 160 gac ggt tcc ctc gag gcg tac aac gtg cat ccg gtg ccg ttg acg acg 528 Asp Gly Ser Leu Glu Ala Tyr Asn Val His Pro Val Pro Leu Thr Thr 165 170 175 ttg acg atc gag gct ttg aag gag ttc ggg ctt tcc cgc aag gag gcc 576 Leu Thr Ile Glu Ala Leu Lys Glu Phe Gly Leu Ser Arg Lys Glu Ala 180 185 190 gag cgg tcg aag aac atg ttc gcg ctc ggg ctt ctg tcc tgg atg tac 624 Glu Arg Ser Lys Asn Met Phe Ala Leu Gly Leu Leu Ser Trp Met Tyr 195 200 205 aac cgt ccg acc gag ggt acg gag aag ttc ctg cgg tcg aag ttc gcc 672 Asn Arg Pro Thr Glu Gly Thr Glu Lys Phe Leu Arg Ser Lys Phe Ala 210 215 220 agg aag ccg gag atc gcc gag gcc aat gtg gcg gct ttc cgc gcg ggc 720 Arg Lys Pro Glu Ile Ala Glu Ala Asn Val Ala Ala Phe Arg Ala Gly 225 230 235 240 tgg aat ttc ggt gag acg acg gag gat ttc gct gtc tcc tac gag gtc 768 Trp Asn Phe Gly Glu Thr Thr Glu Asp Phe Ala Val Ser Tyr Glu Val 245 250 255 gca ccg gcg tca cag gat ttc ccg acg ggc acc tac cgc aat atc tcc 816 Ala Pro Ala Ser Gln Asp Phe Pro Thr Gly Thr Tyr Arg Asn Ile Ser 260 265 270 ggg aat ctc gca ctg tcg tac ggg ctg atc gcg gcg gga cgg cag gcc 864 Gly Asn Leu Ala Leu Ser Tyr Gly Leu Ile Ala Ala Gly Arg Gln Ala 275 280 285 gat ctg ccg gtg tat ctc ggc tcg tat ccg atc act ccg gcg tcc gac 912 Asp Leu Pro Val Tyr Leu Gly Ser Tyr Pro Ile Thr Pro Ala Ser Asp 290 295 300 atc ctg cac gag ctc agc aag cac aag aac ttc ggt gtg cgg acc ttc 960 Ile Leu His Glu Leu Ser Lys His Lys Asn Phe Gly Val Arg Thr Phe 305 310 315 320 cag gcg gag gac gag atc gcc ggg atc ggt gcg gcc ctg ggc gcg tcg 1008 Gln Ala Glu Asp Glu Ile Ala Gly Ile Gly Ala Ala Leu Gly Ala Ser 325 330 335 ttc ggc ggt tca ctg ggt gtg acg acg acg tcg ggc ccg ggt gtg gcg 1056 Phe Gly Gly Ser Leu Gly Val Thr Thr Thr Ser Gly Pro Gly Val Ala 340 345 350 ctg aag tcg gag acg atc ggc ctg gcg gtg tca ctg gaa ctg ccg ctg 1104 Leu Lys Ser Glu Thr Ile Gly Leu Ala Val Ser Leu Glu Leu Pro Leu 355 360 365 ctg atc atc gac atc cag cgc ggc ggc ccc tcc acc ggc ctg ccg acc 1152 Leu Ile Ile Asp Ile Gln Arg Gly Gly Pro Ser Thr Gly Leu Pro Thr 370 375 380 aag acc gag cag gcc gac ctg ctc cag gcc atg tac ggg cgc aac ggc 1200 Lys Thr Glu Gln Ala Asp Leu Leu Gln Ala Met Tyr Gly Arg Asn Gly 385 390 395 400 gag gcc ccg gtc ccg atc gtg gca ccg agg act ccg gcg gac tgc ttc 1248 Glu Ala Pro Val Pro Ile Val Ala Pro Arg Thr Pro Ala Asp Cys Phe 405 410 415 gac gcc gcc ctg gac gcg gcg cgg atc gcg ctg acc tac cgc acc ccg 1296 Asp Ala Ala Leu Asp Ala Ala Arg Ile Ala Leu Thr Tyr Arg Thr Pro 420 425 430 gtc ttc ctg ctc tcg gac ggg tac ctc gcg aac ggc tcc gag ccg tgg 1344 Val Phe Leu Leu Ser Asp Gly Tyr Leu Ala Asn Gly Ser Glu Pro Trp 435 440 445 cgg atc ccc gag gcc gac agc ctc ccc gac ctg cgg aca cgg ttc gcg 1392 Arg Ile Pro Glu Ala Asp Ser Leu Pro Asp Leu Arg Thr Arg Phe Ala 450 455 460 acc ggc ccg aat cac gaa ctc gcg gac ggc acc gag gtg ttc tgg ccc 1440 Thr Gly Pro Asn His Glu Leu Ala Asp Gly Thr Glu Val Phe Trp Pro 465 470 475 480 tac aag agg gac ccc gag acg ctg gcc cgc ccg tgg gcg gtg ccc ggc 1488 Tyr Lys Arg Asp Pro Glu Thr Leu Ala Arg Pro Trp Ala Val Pro Gly 485 490 495 acc ccg ggt ctg gag cac cgg atc ggc ggg atc gag aag cag gac ggc 1536 Thr Pro Gly Leu Glu His Arg Ile Gly Gly Ile Glu Lys Gln Asp Gly 500 505 510 acg ggg aac atc tcc tac gat ccg gcc aac cac gac ttc atg gtc cgc 1584 Thr Gly Asn Ile Ser Tyr Asp Pro Ala Asn His Asp Phe Met Val Arg 515 520 525 acc cgc cag gcc aag atc gac ggc atc cgg gtc ccc gac ctg gag gtc 1632 Thr Arg Gln Ala Lys Ile Asp Gly Ile Arg Val Pro Asp Leu Glu Val 530 535 540 gac gac ccg gcc ggc gcg acg acc ctg gtc ctg ggc tgg ggt tcg acg 1680 Asp Asp Pro Ala Gly Ala Thr Thr Leu Val Leu Gly Trp Gly Ser Thr 545 550 555 560 tac ggg ccg atc acc gcc gcc gtg cgc cgt ctc cgc gcg gcc ggc gag 1728 Tyr Gly Pro Ile Thr Ala Ala Val Arg Arg Leu Arg Ala Ala Gly Glu 565 570 575 acg atc gca cag gca cat ctg cgc cac ctc aat ccc ttc ccc gcc aat 1776 Thr Ile Ala Gln Ala His Leu Arg His Leu Asn Pro Phe Pro Ala Asn 580 585 590 ctc ggt gag gta ctg cgg cgc tac gac aag gtc gtc gtc ccc gag atg 1824 Leu Gly Glu Val Leu Arg Arg Tyr Asp Lys Val Val Val Pro Glu Met 595 600 605 aac ctc ggt cag ctc gcc ctg ctg ctg aga gcc aag tac ctc gtg gac 1872 Asn Leu Gly Gln Leu Ala Leu Leu Leu Arg Ala Lys Tyr Leu Val Asp 610 615 620 gcg cag agt ttc aac cag gtc aac gga atg ccc ttc aag gcg gag cag 1920 Ala Gln Ser Phe Asn Gln Val Asn Gly Met Pro Phe Lys Ala Glu Gln 625 630 635 640 ctc gcc aca gcc ctc aag gag gcc atc gat gcc tga 1956 Leu Ala Thr Ala Leu Lys Glu Ala Ile Asp Ala 645 650 <210> 257 <211> 651 <212> PRT <213> Streptomyces pratensis <400> 257 Met Thr Ser Gln Val Ser Ser Pro Ala Gly Lys Ser Asp Glu Ala Ser 1 5 10 15 Glu Ala Val Val Gly Glu Gln Arg Ala Pro His Ile Ala Gly Ala Gly 20 25 30 Gly Thr Glu Lys Glu Ile Arg Arg Leu Asp Arg Val Ile Ile Arg Phe 35 40 45 Ala Gly Asp Ser Gly Asp Gly Met Gln Leu Thr Gly Asp Arg Phe Thr 50 55 60 Ser Glu Thr Ala Ser Phe Gly Asn Asp Leu Ser Thr Leu Pro Asn Phe 65 70 75 80 Pro Ala Glu Ile Arg Ala Pro Ala Gly Thr Leu Pro Gly Val Ser Ser 85 90 95 Phe Gln Leu His Phe Ala Asp His Asp Ile Leu Thr Pro Gly Asp Ala 100 105 110 Pro Asn Val Leu Val Ala Met Asn Pro Ala Ala Leu Lys Ala Asn Ile 115 120 125 Ala Asp Val Pro Arg Gly Ala Asp Ile Ile Val Asn Thr Asp Glu Phe 130 135 140 Thr Lys Arg Pro Met Ala Lys Val Gly Tyr Ala Glu Ser Pro Leu Glu 145 150 155 160 Asp Gly Ser Leu Glu Ala Tyr Asn Val His Pro Val Pro Leu Thr Thr 165 170 175 Leu Thr Ile Glu Ala Leu Lys Glu Phe Gly Leu Ser Arg Lys Glu Ala 180 185 190 Glu Arg Ser Lys Asn Met Phe Ala Leu Gly Leu Leu Ser Trp Met Tyr 195 200 205 Asn Arg Pro Thr Glu Gly Thr Glu Lys Phe Leu Arg Ser Lys Phe Ala 210 215 220 Arg Lys Pro Glu Ile Ala Glu Ala Asn Val Ala Ala Phe Arg Ala Gly 225 230 235 240 Trp Asn Phe Gly Glu Thr Thr Glu Asp Phe Ala Val Ser Tyr Glu Val 245 250 255 Ala Pro Ala Ser Gln Asp Phe Pro Thr Gly Thr Tyr Arg Asn Ile Ser 260 265 270 Gly Asn Leu Ala Leu Ser Tyr Gly Leu Ile Ala Ala Gly Arg Gln Ala 275 280 285 Asp Leu Pro Val Tyr Leu Gly Ser Tyr Pro Ile Thr Pro Ala Ser Asp 290 295 300 Ile Leu His Glu Leu Ser Lys His Lys Asn Phe Gly Val Arg Thr Phe 305 310 315 320 Gln Ala Glu Asp Glu Ile Ala Gly Ile Gly Ala Ala Leu Gly Ala Ser 325 330 335 Phe Gly Gly Ser Leu Gly Val Thr Thr Thr Ser Gly Pro Gly Val Ala 340 345 350 Leu Lys Ser Glu Thr Ile Gly Leu Ala Val Ser Leu Glu Leu Pro Leu 355 360 365 Leu Ile Ile Asp Ile Gln Arg Gly Gly Pro Ser Thr Gly Leu Pro Thr 370 375 380 Lys Thr Glu Gln Ala Asp Leu Leu Gln Ala Met Tyr Gly Arg Asn Gly 385 390 395 400 Glu Ala Pro Val Pro Ile Val Ala Pro Arg Thr Pro Ala Asp Cys Phe 405 410 415 Asp Ala Ala Leu Asp Ala Ala Arg Ile Ala Leu Thr Tyr Arg Thr Pro 420 425 430 Val Phe Leu Leu Ser Asp Gly Tyr Leu Ala Asn Gly Ser Glu Pro Trp 435 440 445 Arg Ile Pro Glu Ala Asp Ser Leu Pro Asp Leu Arg Thr Arg Phe Ala 450 455 460 Thr Gly Pro Asn His Glu Leu Ala Asp Gly Thr Glu Val Phe Trp Pro 465 470 475 480 Tyr Lys Arg Asp Pro Glu Thr Leu Ala Arg Pro Trp Ala Val Pro Gly 485 490 495 Thr Pro Gly Leu Glu His Arg Ile Gly Gly Ile Glu Lys Gln Asp Gly 500 505 510 Thr Gly Asn Ile Ser Tyr Asp Pro Ala Asn His Asp Phe Met Val Arg 515 520 525 Thr Arg Gln Ala Lys Ile Asp Gly Ile Arg Val Pro Asp Leu Glu Val 530 535 540 Asp Asp Pro Ala Gly Ala Thr Thr Leu Val Leu Gly Trp Gly Ser Thr 545 550 555 560 Tyr Gly Pro Ile Thr Ala Ala Val Arg Arg Leu Arg Ala Ala Gly Glu 565 570 575 Thr Ile Ala Gln Ala His Leu Arg His Leu Asn Pro Phe Pro Ala Asn 580 585 590 Leu Gly Glu Val Leu Arg Arg Tyr Asp Lys Val Val Val Pro Glu Met 595 600 605 Asn Leu Gly Gln Leu Ala Leu Leu Leu Arg Ala Lys Tyr Leu Val Asp 610 615 620 Ala Gln Ser Phe Asn Gln Val Asn Gly Met Pro Phe Lys Ala Glu Gln 625 630 635 640 Leu Ala Thr Ala Leu Lys Glu Ala Ile Asp Ala 645 650 <210> 258 <211> 3768 <212> DNA <213> Propionibacterium freudenreichii <220> <221> CDS <222> (1)..(3768) <223> RM25_0186 gene from Propionibacterium freudenreichii DSM 20271encoding pyruvate-flavodoxin/ferredoxin oxidoreductase <400> 258 atg act aca act acc cgt ggg ccg gtt ccc ggc tcg aat ggc atg ccc 48 Met Thr Thr Thr Thr Arg Gly Pro Val Pro Gly Ser Asn Gly Met Pro 1 5 10 15 gcc aat cca ggt ctg agc ggc gag gcc gcc acc gca acc ccg tca ccc 96 Ala Asn Pro Gly Leu Ser Gly Glu Ala Ala Thr Ala Thr Pro Ser Pro 20 25 30 gtt gac gtc gct gcc ggc gcc aag gac gct gcc gat gag ctg gcc cag 144 Val Asp Val Ala Ala Gly Ala Lys Asp Ala Ala Asp Glu Leu Ala Gln 35 40 45 tca cga cgc gag cag gac atc acc cat cag atg atc tgc gac ggc aac 192 Ser Arg Arg Glu Gln Asp Ile Thr His Gln Met Ile Cys Asp Gly Asn 50 55 60 acc gcc gcc tct gat gtg gcc ttc cgc atc aat gag ctg tgc tcg atc 240 Thr Ala Ala Ser Asp Val Ala Phe Arg Ile Asn Glu Leu Cys Ser Ile 65 70 75 80 tac ccg atc acg ccg agc tcc ccg atg gcc gaa ctg gcc gac gag tgg 288 Tyr Pro Ile Thr Pro Ser Ser Pro Met Ala Glu Leu Ala Asp Glu Trp 85 90 95 agt gcc cgc gac cgc atg aac atc tgg ggc cag gtg ccc cat gtg atg 336 Ser Ala Arg Asp Arg Met Asn Ile Trp Gly Gln Val Pro His Val Met 100 105 110 gag atg cag tcg gag gcc ggc gcg gcc ggt gcc atg cac ggc tcc ctg 384 Glu Met Gln Ser Glu Ala Gly Ala Ala Gly Ala Met His Gly Ser Leu 115 120 125 cag ggc ggc gcc ctg gcg acc acc ttc acg gcg tcg cag ggc ctg ctg 432 Gln Gly Gly Ala Leu Ala Thr Thr Phe Thr Ala Ser Gln Gly Leu Leu 130 135 140 ctg atg atc ccg aac atg tac aag atc gcc ggt gag ctc acc tcc acg 480 Leu Met Ile Pro Asn Met Tyr Lys Ile Ala Gly Glu Leu Thr Ser Thr 145 150 155 160 gtg atg cac gtc gcc gcg cgc tcg ctg gcc acc cag ggc ctg tcg atc 528 Val Met His Val Ala Ala Arg Ser Leu Ala Thr Gln Gly Leu Ser Ile 165 170 175 ttc ggt gat cac cag gac gtg atg gcc tgt cgc cag acc ggt tgg gcg 576 Phe Gly Asp His Gln Asp Val Met Ala Cys Arg Gln Thr Gly Trp Ala 180 185 190 atg ctg tgc tcc acc ggc gtg cag cag tgc cat gac aat gcc ctg atc 624 Met Leu Cys Ser Thr Gly Val Gln Gln Cys His Asp Asn Ala Leu Ile 195 200 205 tcc cag gtc gcc acg ctg cgt tcg cgc gtg ccg ttc atg cac ttc ttc 672 Ser Gln Val Ala Thr Leu Arg Ser Arg Val Pro Phe Met His Phe Phe 210 215 220 gac ggc ttc cgc acc agc cat gag ctc aac acc tgc atc cag ctc acc 720 Asp Gly Phe Arg Thr Ser His Glu Leu Asn Thr Cys Ile Gln Leu Thr 225 230 235 240 gac gac cag ctg cgt tcg atg gtg ccc gat gcg ctc gtg cgc gag cac 768 Asp Asp Gln Leu Arg Ser Met Val Pro Asp Ala Leu Val Arg Glu His 245 250 255 cgc gag cgg gcc ctg tcg ccc gac aac ccg ttc atc cgt ggc acc gcc 816 Arg Glu Arg Ala Leu Ser Pro Asp Asn Pro Phe Ile Arg Gly Thr Ala 260 265 270 cag aac gcc gac gtg tac ttc cag ggc cgc gag gcc ggc aac aag tac 864 Gln Asn Ala Asp Val Tyr Phe Gln Gly Arg Glu Ala Gly Asn Lys Tyr 275 280 285 tac gac tcg gtt ccg ggc atc gtg cag gac gcg atg gac gag ttc gcc 912 Tyr Asp Ser Val Pro Gly Ile Val Gln Asp Ala Met Asp Glu Phe Ala 290 295 300 gcc atg acc ggc cgc cag tac cac ctg gcc gac tac tac ggc gcg ccc 960 Ala Met Thr Gly Arg Gln Tyr His Leu Ala Asp Tyr Tyr Gly Ala Pro 305 310 315 320 gac gcc gat cgc gtc atc gtg atc atg ggc tcg ggt gcc gag acc gtg 1008 Asp Ala Asp Arg Val Ile Val Ile Met Gly Ser Gly Ala Glu Thr Val 325 330 335 cag cag acc gtc agc aag ctc aat gag cag ggc gag aag gtc ggc ctg 1056 Gln Gln Thr Val Ser Lys Leu Asn Glu Gln Gly Glu Lys Val Gly Leu 340 345 350 gtg gtc atc cgc ctg tac cgt ccg ttc ccg acg cag gcc gtg ctg gac 1104 Val Val Ile Arg Leu Tyr Arg Pro Phe Pro Thr Gln Ala Val Leu Asp 355 360 365 tgc att ccc gca tcg gtc aag aag atc gcc gtg ctc gac cgc acc aag 1152 Cys Ile Pro Ala Ser Val Lys Lys Ile Ala Val Leu Asp Arg Thr Lys 370 375 380 gag ccg ggc tcc aac ggt gag ccc ctg ttc ctc gac gtg gtc tcg gca 1200 Glu Pro Gly Ser Asn Gly Glu Pro Leu Phe Leu Asp Val Val Ser Ala 385 390 395 400 gtc tcc gag gcc tat tcg aac ggc gag cgc gac aac ctg ccc gcc atc 1248 Val Ser Glu Ala Tyr Ser Asn Gly Glu Arg Asp Asn Leu Pro Ala Ile 405 410 415 atc ggt ggc cgc tac ggc ctg tcg agc aag gag ttc acg ccg ggc atg 1296 Ile Gly Gly Arg Tyr Gly Leu Ser Ser Lys Glu Phe Thr Pro Gly Met 420 425 430 tgc gcc gcc gtg tac gac gag ctc gcc aag gac aag ccg aag cgt cgc 1344 Cys Ala Ala Val Tyr Asp Glu Leu Ala Lys Asp Lys Pro Lys Arg Arg 435 440 445 ttc acc gtc ggc atc acc gac gat gtg acg cac ctg tcg atc ccg tgg 1392 Phe Thr Val Gly Ile Thr Asp Asp Val Thr His Leu Ser Ile Pro Trp 450 455 460 gac gcc tcg ctc gac ctg gag gac ccc gag acc tcg cgc gca gtg ttc 1440 Asp Ala Ser Leu Asp Leu Glu Asp Pro Glu Thr Ser Arg Ala Val Phe 465 470 475 480 tac ggc atc ggt gct gac ggc acc gtc ggc gcc aac aag aac acc atc 1488 Tyr Gly Ile Gly Ala Asp Gly Thr Val Gly Ala Asn Lys Asn Thr Ile 485 490 495 aag atc ctc ggc tcc gag ccg ggc acc tac gcg cag ggc tac ttc gtc 1536 Lys Ile Leu Gly Ser Glu Pro Gly Thr Tyr Ala Gln Gly Tyr Phe Val 500 505 510 tac gac tcg aag aag tcc ggc ggc cgc acc acc tcg cac ctt cgc ttc 1584 Tyr Asp Ser Lys Lys Ser Gly Gly Arg Thr Thr Ser His Leu Arg Phe 515 520 525 gga ccc gat ccg atc aag gcc ccc tac ctg gtg aac cag gcc ggc ttc 1632 Gly Pro Asp Pro Ile Lys Ala Pro Tyr Leu Val Asn Gln Ala Gly Phe 530 535 540 atc ggc gtg cac cac tgg gcc gac ctt gag cgc atc gac gtg ctg gcg 1680 Ile Gly Val His His Trp Ala Asp Leu Glu Arg Ile Asp Val Leu Ala 545 550 555 560 ttc gcc cgc aag ggc acc acg gtg ctg atc aac agc ccg tac ccc gcc 1728 Phe Ala Arg Lys Gly Thr Thr Val Leu Ile Asn Ser Pro Tyr Pro Ala 565 570 575 gag gac gtc tgg ggc cat ctg ccg gcc ccg atg cag aag aag atc atc 1776 Glu Asp Val Trp Gly His Leu Pro Ala Pro Met Gln Lys Lys Ile Ile 580 585 590 gac ctc gac ctg cag gtg tat gcg atc gac gcc ggt gag gtg gcc cgt 1824 Asp Leu Asp Leu Gln Val Tyr Ala Ile Asp Ala Gly Glu Val Ala Arg 595 600 605 tcg gtg ggc ctg ggc aac cgc acc aac acg gtg ctg cag acc tgc tac 1872 Ser Val Gly Leu Gly Asn Arg Thr Asn Thr Val Leu Gln Thr Cys Tyr 610 615 620 ttc aag atc agt ggc gtg ctt ccc gag gac cac gcg atc gag gcc atc 1920 Phe Lys Ile Ser Gly Val Leu Pro Glu Asp His Ala Ile Glu Ala Ile 625 630 635 640 aag aac tcg atc acc aag acc tac gcg aag aag tcg atg gag atc gtg 1968 Lys Asn Ser Ile Thr Lys Thr Tyr Ala Lys Lys Ser Met Glu Ile Val 645 650 655 gag aag aac cac gcc gcc gtc gac gcc gcc ctg gag cac ctg cac aag 2016 Glu Lys Asn His Ala Ala Val Asp Ala Ala Leu Glu His Leu His Lys 660 665 670 atc gac gtg ccg gcc aag gtc acc tcc acc gag gac tac ctg ccg ccc 2064 Ile Asp Val Pro Ala Lys Val Thr Ser Thr Glu Asp Tyr Leu Pro Pro 675 680 685 gtg ccg tcg ttc gcg cct gac ttc gtc aag gac gtc acc gcg gcc atg 2112 Val Pro Ser Phe Ala Pro Asp Phe Val Lys Asp Val Thr Ala Ala Met 690 695 700 atg acc gag cag ggc gag tcg ctg ccg gtg agc aag ctg ccg gcc gat 2160 Met Thr Glu Gln Gly Glu Ser Leu Pro Val Ser Lys Leu Pro Ala Asp 705 710 715 720 ggt tcg ttc ccc tcg ggc acc acg cag tac gag aag cgc aat gtg tcc 2208 Gly Ser Phe Pro Ser Gly Thr Thr Gln Tyr Glu Lys Arg Asn Val Ser 725 730 735 gag atc atc gcg gtc tgg gac cag gac aac tgc atc cag tgc ggc aac 2256 Glu Ile Ile Ala Val Trp Asp Gln Asp Asn Cys Ile Gln Cys Gly Asn 740 745 750 tgc gcc ttc gtc tgc ccg cac ggc gtg ctg agg gcc aag tac tac aag 2304 Cys Ala Phe Val Cys Pro His Gly Val Leu Arg Ala Lys Tyr Tyr Lys 755 760 765 ccc gat gtg ctc gac gat gcg ccg aag tcg ttc cag gcg gtt ccg ctg 2352 Pro Asp Val Leu Asp Asp Ala Pro Lys Ser Phe Gln Ala Val Pro Leu 770 775 780 aat gcg gcc ggc ctg ccc gac gag atg tac acc ctg cag gtg ttc gcc 2400 Asn Ala Ala Gly Leu Pro Asp Glu Met Tyr Thr Leu Gln Val Phe Ala 785 790 795 800 gag gac tgc acc ggt tgt ggc ctg tgc gtc gag gcc tgc ccc gtg cat 2448 Glu Asp Cys Thr Gly Cys Gly Leu Cys Val Glu Ala Cys Pro Val His 805 810 815 ccc atc ggt ggc gac ccc gaa tgc aag gcg atc aac ctg gat tcc gtg 2496 Pro Ile Gly Gly Asp Pro Glu Cys Lys Ala Ile Asn Leu Asp Ser Val 820 825 830 ctc gac cgc acc aac gag cgg gcg aac gtg gag ttc ttc cag aag atc 2544 Leu Asp Arg Thr Asn Glu Arg Ala Asn Val Glu Phe Phe Gln Lys Ile 835 840 845 ccc gag ccc ccg cgc acc cgc gtg aac tac ggt gcc gtg cgt ggc gcc 2592 Pro Glu Pro Pro Arg Thr Arg Val Asn Tyr Gly Ala Val Arg Gly Ala 850 855 860 cag ttc ctg cag ccg ctg ttc gag ttc agc ggt gcc tgc ccg ggt tgt 2640 Gln Phe Leu Gln Pro Leu Phe Glu Phe Ser Gly Ala Cys Pro Gly Cys 865 870 875 880 ggc gag acg ccg tac ctc aag ctg ctc acc cag ctg ttc ggc gac cgc 2688 Gly Glu Thr Pro Tyr Leu Lys Leu Leu Thr Gln Leu Phe Gly Asp Arg 885 890 895 gcc acc gtg gcg aat gcc acc ggc tgc tcg tcc atc tac ggc ggc aac 2736 Ala Thr Val Ala Asn Ala Thr Gly Cys Ser Ser Ile Tyr Gly Gly Asn 900 905 910 ctg ccg acc acc ccg tgg gcg aag aac aag gag gga cgc ggc ccg gcc 2784 Leu Pro Thr Thr Pro Trp Ala Lys Asn Lys Glu Gly Arg Gly Pro Ala 915 920 925 tgg agc aac tca ttg ttc gag gac aac gcc gag ttc ggc ctt ggc atg 2832 Trp Ser Asn Ser Leu Phe Glu Asp Asn Ala Glu Phe Gly Leu Gly Met 930 935 940 cgc ctg gcg gcc gac ctg cac aac gaa ctg gcc cgt cag cgc gtt gac 2880 Arg Leu Ala Ala Asp Leu His Asn Glu Leu Ala Arg Gln Arg Val Asp 945 950 955 960 gag ctg tcc gat gcg atc aac gac ccc gag ctg gtc gat cag ctg ctg 2928 Glu Leu Ser Asp Ala Ile Asn Asp Pro Glu Leu Val Asp Gln Leu Leu 965 970 975 aac gcc ccg cag gcg cag gag tcc gat ctg cac gcc cag gcc gag cgc 2976 Asn Ala Pro Gln Ala Gln Glu Ser Asp Leu His Ala Gln Ala Glu Arg 980 985 990 gtc gac gcc ctg cag gat cgc ctg acc gac ctg gtc aac gat ccg aac 3024 Val Asp Ala Leu Gln Asp Arg Leu Thr Asp Leu Val Asn Asp Pro Asn 995 1000 1005 gtg gac gcc gac acc aag gcc aag gtc gag gac ctg cgg tcg gtg 3069 Val Asp Ala Asp Thr Lys Ala Lys Val Glu Asp Leu Arg Ser Val 1010 1015 1020 gcc gac aac ctg ctg cgt cgt tcc gtg tgg atc gtc ggc ggc gac 3114 Ala Asp Asn Leu Leu Arg Arg Ser Val Trp Ile Val Gly Gly Asp 1025 1030 1035 ggt tgg gcc tac gac atc ggt tcg ggc ggc ctt gac cat gtg ctg 3159 Gly Trp Ala Tyr Asp Ile Gly Ser Gly Gly Leu Asp His Val Leu 1040 1045 1050 tcc acc gga cgc aat gtc aat gtg ctg gtg ctc gac acc gag gtc 3204 Ser Thr Gly Arg Asn Val Asn Val Leu Val Leu Asp Thr Glu Val 1055 1060 1065 tac tcc aat acc ggt ggc cag gcc tcg aag tcg tcg ccc atg ggt 3249 Tyr Ser Asn Thr Gly Gly Gln Ala Ser Lys Ser Ser Pro Met Gly 1070 1075 1080 gcg atc gcg aag ttc gcg acc gcc ggc aag cgc acg aac aag aag 3294 Ala Ile Ala Lys Phe Ala Thr Ala Gly Lys Arg Thr Asn Lys Lys 1085 1090 1095 gac atc gcc atg cag gcc gtg tcc tac ggc gac gtc tat gtc gcc 3339 Asp Ile Ala Met Gln Ala Val Ser Tyr Gly Asp Val Tyr Val Ala 1100 1105 1110 cgc gtg gcg ttc ggt gcc gac ccg gag cag acg ctg aag gca ttc 3384 Arg Val Ala Phe Gly Ala Asp Pro Glu Gln Thr Leu Lys Ala Phe 1115 1120 1125 cgt gag gcc gag gcc tac ccc ggc ccc agc ctg atc atc gcc tac 3429 Arg Glu Ala Glu Ala Tyr Pro Gly Pro Ser Leu Ile Ile Ala Tyr 1130 1135 1140 agc cac tgc atc agc cat ggc tac aac ctg cgc aag ggc ctg gac 3474 Ser His Cys Ile Ser His Gly Tyr Asn Leu Arg Lys Gly Leu Asp 1145 1150 1155 cag cag tac aag gca gtg gcc tcc ggt cac tgg ccg ctg atc cgg 3519 Gln Gln Tyr Lys Ala Val Ala Ser Gly His Trp Pro Leu Ile Arg 1160 1165 1170 tac aac ccg gag gtt cgc gac tcg ggt ggc aac ccg ttc ctg ctc 3564 Tyr Asn Pro Glu Val Arg Asp Ser Gly Gly Asn Pro Phe Leu Leu 1175 1180 1185 gac tcg gcc cgt ccg cgc atc tcg ctg atg gac tac cgc aag acc 3609 Asp Ser Ala Arg Pro Arg Ile Ser Leu Met Asp Tyr Arg Lys Thr 1190 1195 1200 gag ctg cgc ttc aag atg ctg atg gtc aag gat ccg gaa gag gcc 3654 Glu Leu Arg Phe Lys Met Leu Met Val Lys Asp Pro Glu Glu Ala 1205 1210 1215 aag cac ctc aat gac ctc agc cag gag cag gtg acc agg cgt ttc 3699 Lys His Leu Asn Asp Leu Ser Gln Glu Gln Val Thr Arg Arg Phe 1220 1225 1230 gcc gac tac gag gaa atg gcc tca cgt ccg gcc gag atg ttc gcc 3744 Ala Asp Tyr Glu Glu Met Ala Ser Arg Pro Ala Glu Met Phe Ala 1235 1240 1245 acc gac gca cgg agg gat gtc tga 3768 Thr Asp Ala Arg Arg Asp Val 1250 1255 <210> 259 <211> 1255 <212> PRT <213> Propionibacterium freudenreichii <400> 259 Met Thr Thr Thr Thr Arg Gly Pro Val Pro Gly Ser Asn Gly Met Pro 1 5 10 15 Ala Asn Pro Gly Leu Ser Gly Glu Ala Ala Thr Ala Thr Pro Ser Pro 20 25 30 Val Asp Val Ala Ala Gly Ala Lys Asp Ala Ala Asp Glu Leu Ala Gln 35 40 45 Ser Arg Arg Glu Gln Asp Ile Thr His Gln Met Ile Cys Asp Gly Asn 50 55 60 Thr Ala Ala Ser Asp Val Ala Phe Arg Ile Asn Glu Leu Cys Ser Ile 65 70 75 80 Tyr Pro Ile Thr Pro Ser Ser Pro Met Ala Glu Leu Ala Asp Glu Trp 85 90 95 Ser Ala Arg Asp Arg Met Asn Ile Trp Gly Gln Val Pro His Val Met 100 105 110 Glu Met Gln Ser Glu Ala Gly Ala Ala Gly Ala Met His Gly Ser Leu 115 120 125 Gln Gly Gly Ala Leu Ala Thr Thr Phe Thr Ala Ser Gln Gly Leu Leu 130 135 140 Leu Met Ile Pro Asn Met Tyr Lys Ile Ala Gly Glu Leu Thr Ser Thr 145 150 155 160 Val Met His Val Ala Ala Arg Ser Leu Ala Thr Gln Gly Leu Ser Ile 165 170 175 Phe Gly Asp His Gln Asp Val Met Ala Cys Arg Gln Thr Gly Trp Ala 180 185 190 Met Leu Cys Ser Thr Gly Val Gln Gln Cys His Asp Asn Ala Leu Ile 195 200 205 Ser Gln Val Ala Thr Leu Arg Ser Arg Val Pro Phe Met His Phe Phe 210 215 220 Asp Gly Phe Arg Thr Ser His Glu Leu Asn Thr Cys Ile Gln Leu Thr 225 230 235 240 Asp Asp Gln Leu Arg Ser Met Val Pro Asp Ala Leu Val Arg Glu His 245 250 255 Arg Glu Arg Ala Leu Ser Pro Asp Asn Pro Phe Ile Arg Gly Thr Ala 260 265 270 Gln Asn Ala Asp Val Tyr Phe Gln Gly Arg Glu Ala Gly Asn Lys Tyr 275 280 285 Tyr Asp Ser Val Pro Gly Ile Val Gln Asp Ala Met Asp Glu Phe Ala 290 295 300 Ala Met Thr Gly Arg Gln Tyr His Leu Ala Asp Tyr Tyr Gly Ala Pro 305 310 315 320 Asp Ala Asp Arg Val Ile Val Ile Met Gly Ser Gly Ala Glu Thr Val 325 330 335 Gln Gln Thr Val Ser Lys Leu Asn Glu Gln Gly Glu Lys Val Gly Leu 340 345 350 Val Val Ile Arg Leu Tyr Arg Pro Phe Pro Thr Gln Ala Val Leu Asp 355 360 365 Cys Ile Pro Ala Ser Val Lys Lys Ile Ala Val Leu Asp Arg Thr Lys 370 375 380 Glu Pro Gly Ser Asn Gly Glu Pro Leu Phe Leu Asp Val Val Ser Ala 385 390 395 400 Val Ser Glu Ala Tyr Ser Asn Gly Glu Arg Asp Asn Leu Pro Ala Ile 405 410 415 Ile Gly Gly Arg Tyr Gly Leu Ser Ser Lys Glu Phe Thr Pro Gly Met 420 425 430 Cys Ala Ala Val Tyr Asp Glu Leu Ala Lys Asp Lys Pro Lys Arg Arg 435 440 445 Phe Thr Val Gly Ile Thr Asp Asp Val Thr His Leu Ser Ile Pro Trp 450 455 460 Asp Ala Ser Leu Asp Leu Glu Asp Pro Glu Thr Ser Arg Ala Val Phe 465 470 475 480 Tyr Gly Ile Gly Ala Asp Gly Thr Val Gly Ala Asn Lys Asn Thr Ile 485 490 495 Lys Ile Leu Gly Ser Glu Pro Gly Thr Tyr Ala Gln Gly Tyr Phe Val 500 505 510 Tyr Asp Ser Lys Lys Ser Gly Gly Arg Thr Thr Ser His Leu Arg Phe 515 520 525 Gly Pro Asp Pro Ile Lys Ala Pro Tyr Leu Val Asn Gln Ala Gly Phe 530 535 540 Ile Gly Val His His Trp Ala Asp Leu Glu Arg Ile Asp Val Leu Ala 545 550 555 560 Phe Ala Arg Lys Gly Thr Thr Val Leu Ile Asn Ser Pro Tyr Pro Ala 565 570 575 Glu Asp Val Trp Gly His Leu Pro Ala Pro Met Gln Lys Lys Ile Ile 580 585 590 Asp Leu Asp Leu Gln Val Tyr Ala Ile Asp Ala Gly Glu Val Ala Arg 595 600 605 Ser Val Gly Leu Gly Asn Arg Thr Asn Thr Val Leu Gln Thr Cys Tyr 610 615 620 Phe Lys Ile Ser Gly Val Leu Pro Glu Asp His Ala Ile Glu Ala Ile 625 630 635 640 Lys Asn Ser Ile Thr Lys Thr Tyr Ala Lys Lys Ser Met Glu Ile Val 645 650 655 Glu Lys Asn His Ala Ala Val Asp Ala Ala Leu Glu His Leu His Lys 660 665 670 Ile Asp Val Pro Ala Lys Val Thr Ser Thr Glu Asp Tyr Leu Pro Pro 675 680 685 Val Pro Ser Phe Ala Pro Asp Phe Val Lys Asp Val Thr Ala Ala Met 690 695 700 Met Thr Glu Gln Gly Glu Ser Leu Pro Val Ser Lys Leu Pro Ala Asp 705 710 715 720 Gly Ser Phe Pro Ser Gly Thr Thr Gln Tyr Glu Lys Arg Asn Val Ser 725 730 735 Glu Ile Ile Ala Val Trp Asp Gln Asp Asn Cys Ile Gln Cys Gly Asn 740 745 750 Cys Ala Phe Val Cys Pro His Gly Val Leu Arg Ala Lys Tyr Tyr Lys 755 760 765 Pro Asp Val Leu Asp Asp Ala Pro Lys Ser Phe Gln Ala Val Pro Leu 770 775 780 Asn Ala Ala Gly Leu Pro Asp Glu Met Tyr Thr Leu Gln Val Phe Ala 785 790 795 800 Glu Asp Cys Thr Gly Cys Gly Leu Cys Val Glu Ala Cys Pro Val His 805 810 815 Pro Ile Gly Gly Asp Pro Glu Cys Lys Ala Ile Asn Leu Asp Ser Val 820 825 830 Leu Asp Arg Thr Asn Glu Arg Ala Asn Val Glu Phe Phe Gln Lys Ile 835 840 845 Pro Glu Pro Pro Arg Thr Arg Val Asn Tyr Gly Ala Val Arg Gly Ala 850 855 860 Gln Phe Leu Gln Pro Leu Phe Glu Phe Ser Gly Ala Cys Pro Gly Cys 865 870 875 880 Gly Glu Thr Pro Tyr Leu Lys Leu Leu Thr Gln Leu Phe Gly Asp Arg 885 890 895 Ala Thr Val Ala Asn Ala Thr Gly Cys Ser Ser Ile Tyr Gly Gly Asn 900 905 910 Leu Pro Thr Thr Pro Trp Ala Lys Asn Lys Glu Gly Arg Gly Pro Ala 915 920 925 Trp Ser Asn Ser Leu Phe Glu Asp Asn Ala Glu Phe Gly Leu Gly Met 930 935 940 Arg Leu Ala Ala Asp Leu His Asn Glu Leu Ala Arg Gln Arg Val Asp 945 950 955 960 Glu Leu Ser Asp Ala Ile Asn Asp Pro Glu Leu Val Asp Gln Leu Leu 965 970 975 Asn Ala Pro Gln Ala Gln Glu Ser Asp Leu His Ala Gln Ala Glu Arg 980 985 990 Val Asp Ala Leu Gln Asp Arg Leu Thr Asp Leu Val Asn Asp Pro Asn 995 1000 1005 Val Asp Ala Asp Thr Lys Ala Lys Val Glu Asp Leu Arg Ser Val 1010 1015 1020 Ala Asp Asn Leu Leu Arg Arg Ser Val Trp Ile Val Gly Gly Asp 1025 1030 1035 Gly Trp Ala Tyr Asp Ile Gly Ser Gly Gly Leu Asp His Val Leu 1040 1045 1050 Ser Thr Gly Arg Asn Val Asn Val Leu Val Leu Asp Thr Glu Val 1055 1060 1065 Tyr Ser Asn Thr Gly Gly Gln Ala Ser Lys Ser Ser Pro Met Gly 1070 1075 1080 Ala Ile Ala Lys Phe Ala Thr Ala Gly Lys Arg Thr Asn Lys Lys 1085 1090 1095 Asp Ile Ala Met Gln Ala Val Ser Tyr Gly Asp Val Tyr Val Ala 1100 1105 1110 Arg Val Ala Phe Gly Ala Asp Pro Glu Gln Thr Leu Lys Ala Phe 1115 1120 1125 Arg Glu Ala Glu Ala Tyr Pro Gly Pro Ser Leu Ile Ile Ala Tyr 1130 1135 1140 Ser His Cys Ile Ser His Gly Tyr Asn Leu Arg Lys Gly Leu Asp 1145 1150 1155 Gln Gln Tyr Lys Ala Val Ala Ser Gly His Trp Pro Leu Ile Arg 1160 1165 1170 Tyr Asn Pro Glu Val Arg Asp Ser Gly Gly Asn Pro Phe Leu Leu 1175 1180 1185 Asp Ser Ala Arg Pro Arg Ile Ser Leu Met Asp Tyr Arg Lys Thr 1190 1195 1200 Glu Leu Arg Phe Lys Met Leu Met Val Lys Asp Pro Glu Glu Ala 1205 1210 1215 Lys His Leu Asn Asp Leu Ser Gln Glu Gln Val Thr Arg Arg Phe 1220 1225 1230 Ala Asp Tyr Glu Glu Met Ala Ser Arg Pro Ala Glu Met Phe Ala 1235 1240 1245 Thr Asp Ala Arg Arg Asp Val 1250 1255 <210> 260 <211> 3600 <212> DNA <213> Synechocystis PCC6803 <220> <221> CDS <222> (1)..(3600) <223> nifJ gene from Synechocystis sp. PCC 6803 encoding pyruvate-flavodoxin/ferredoxin oxidoreductase <400> 260 atg agt tta cct acc tat gcc acc ctc gac ggt aat gaa gcg gtg gcc 48 Met Ser Leu Pro Thr Tyr Ala Thr Leu Asp Gly Asn Glu Ala Val Ala 1 5 10 15 cgt gtg gcc tac ctg ctc agt gaa gtg att gcc att tat ccc atc acc 96 Arg Val Ala Tyr Leu Leu Ser Glu Val Ile Ala Ile Tyr Pro Ile Thr 20 25 30 cct tcc tcg ccc atg ggg gaa tgg tcc gat gct tgg gca gca gaa cac 144 Pro Ser Ser Pro Met Gly Glu Trp Ser Asp Ala Trp Ala Ala Glu His 35 40 45 cgg ccc aat ttg tgg ggc acc gta cca ttg gtg gtg gaa atg caa agc 192 Arg Pro Asn Leu Trp Gly Thr Val Pro Leu Val Val Glu Met Gln Ser 50 55 60 gag ggg gga gcc gcc ggt act gtc cat ggc gct ctg caa tcg gga gct 240 Glu Gly Gly Ala Ala Gly Thr Val His Gly Ala Leu Gln Ser Gly Ala 65 70 75 80 ttg acc aca aca ttt acc gct tcc cag ggc tta atg ttg atg ttg ccc 288 Leu Thr Thr Thr Phe Thr Ala Ser Gln Gly Leu Met Leu Met Leu Pro 85 90 95 aat atg cac aaa att gct ggg gaa tta aca gcc atg gtt ttg cat gtg 336 Asn Met His Lys Ile Ala Gly Glu Leu Thr Ala Met Val Leu His Val 100 105 110 gcg gcc cgt tct tta gcg gcc cag ggc cta tct att ttt ggg gat cac 384 Ala Ala Arg Ser Leu Ala Ala Gln Gly Leu Ser Ile Phe Gly Asp His 115 120 125 agt gat gtg atg gcg gcc aga aat acg ggc ttt gcc atg tta agt tcc 432 Ser Asp Val Met Ala Ala Arg Asn Thr Gly Phe Ala Met Leu Ser Ser 130 135 140 aat tct gtc cag gaa gcc cac gat ttt gcc ctc att gcc acg gcc acc 480 Asn Ser Val Gln Glu Ala His Asp Phe Ala Leu Ile Ala Thr Ala Thr 145 150 155 160 agc ttt gcc acc agg ata ccg gga ctg cac ttt ttt gat ggt ttt cgc 528 Ser Phe Ala Thr Arg Ile Pro Gly Leu His Phe Phe Asp Gly Phe Arg 165 170 175 act tcc cac gaa gaa caa aaa att gag ctt tta ccc cag gaa gta ctc 576 Thr Ser His Glu Glu Gln Lys Ile Glu Leu Leu Pro Gln Glu Val Leu 180 185 190 cgt ggt ttg att aag gat gag gat gtg cta gcc cac cgg gga cgg gct 624 Arg Gly Leu Ile Lys Asp Glu Asp Val Leu Ala His Arg Gly Arg Ala 195 200 205 ttg acc ccc gat cgc ccg aag ttg cgg ggg acg gcc caa aat ccg gat 672 Leu Thr Pro Asp Arg Pro Lys Leu Arg Gly Thr Ala Gln Asn Pro Asp 210 215 220 gtc tat ttc caa gct agg gaa acg gtt aat ccc ttt tat gcc agt tat 720 Val Tyr Phe Gln Ala Arg Glu Thr Val Asn Pro Phe Tyr Ala Ser Tyr 225 230 235 240 ccc aac gtg ctg gag cag gtg atg gaa caa ttt ggc cag cta acc ggc 768 Pro Asn Val Leu Glu Gln Val Met Glu Gln Phe Gly Gln Leu Thr Gly 245 250 255 cgc cat tac cgt ccc tat gaa tat tgt ggc cat ccg gaa gcg gaa cgg 816 Arg His Tyr Arg Pro Tyr Glu Tyr Cys Gly His Pro Glu Ala Glu Arg 260 265 270 gtg att gtg ctg atg ggt tct ggt gcg gaa acg gcc cag gaa acg gtg 864 Val Ile Val Leu Met Gly Ser Gly Ala Glu Thr Ala Gln Glu Thr Val 275 280 285 gat ttt cta act gcc caa ggg gaa aag gtt ggt tta ctg aaa gta cgc 912 Asp Phe Leu Thr Ala Gln Gly Glu Lys Val Gly Leu Leu Lys Val Arg 290 295 300 ctc tat cgg ccc ttt gct ggc gat cgc ctg gtt aat gct cta cca aaa 960 Leu Tyr Arg Pro Phe Ala Gly Asp Arg Leu Val Asn Ala Leu Pro Lys 305 310 315 320 acg gtg caa aaa ata gcg gtg ctg gac cgg tgt aag gaa ccg ggg agc 1008 Thr Val Gln Lys Ile Ala Val Leu Asp Arg Cys Lys Glu Pro Gly Ser 325 330 335 att ggg gaa ccc ctc tat cag gat gtg ctg acg gcc ttt ttt gaa gcg 1056 Ile Gly Glu Pro Leu Tyr Gln Asp Val Leu Thr Ala Phe Phe Glu Ala 340 345 350 ggc atg atg ccg aaa att att ggt ggc cgt tac ggt ctg tca tcc aag 1104 Gly Met Met Pro Lys Ile Ile Gly Gly Arg Tyr Gly Leu Ser Ser Lys 355 360 365 gaa ttt acc ccc gcc atg gtt aaa ggg gtg ttg gac cat tta aat caa 1152 Glu Phe Thr Pro Ala Met Val Lys Gly Val Leu Asp His Leu Asn Gln 370 375 380 acc aac ccc aaa aac cat ttc acc gta ggc att aac gat gat ttg agc 1200 Thr Asn Pro Lys Asn His Phe Thr Val Gly Ile Asn Asp Asp Leu Ser 385 390 395 400 cac acc agc atc gac tat gac ccc agt ttt tcc acg gaa gca gat tct 1248 His Thr Ser Ile Asp Tyr Asp Pro Ser Phe Ser Thr Glu Ala Asp Ser 405 410 415 gtc gtc cgg gca att ttc tac ggt ctc ggt tcc gac ggt acg gtg ggg 1296 Val Val Arg Ala Ile Phe Tyr Gly Leu Gly Ser Asp Gly Thr Val Gly 420 425 430 gcc aat aag aac tcc atc aaa atc att ggc gaa gat acg gat aac tac 1344 Ala Asn Lys Asn Ser Ile Lys Ile Ile Gly Glu Asp Thr Asp Asn Tyr 435 440 445 gcc cag ggt tat ttt gtt tac gac tcg aaa aaa tcc ggt tct gta acc 1392 Ala Gln Gly Tyr Phe Val Tyr Asp Ser Lys Lys Ser Gly Ser Val Thr 450 455 460 gtt tcc cat ctg cgc ttt ggc cct aat ccc atc ctg tcc act tac ctg 1440 Val Ser His Leu Arg Phe Gly Pro Asn Pro Ile Leu Ser Thr Tyr Leu 465 470 475 480 att agc caa gcc aat ttt gtc gcc tgt cac cag tgg gaa ttt ttg gaa 1488 Ile Ser Gln Ala Asn Phe Val Ala Cys His Gln Trp Glu Phe Leu Glu 485 490 495 cag ttt gaa gtc ttg gaa cca gcc gtt gat ggc ggc gtt ttc ctg gtc 1536 Gln Phe Glu Val Leu Glu Pro Ala Val Asp Gly Gly Val Phe Leu Val 500 505 510 aat agc ccc tac ggc cca gag gaa att tgg cga gag ttt ccc cgc aaa 1584 Asn Ser Pro Tyr Gly Pro Glu Glu Ile Trp Arg Glu Phe Pro Arg Lys 515 520 525 gta caa cag gaa att att gac aaa aat ctc aag gtt tac acc atc aat 1632 Val Gln Gln Glu Ile Ile Asp Lys Asn Leu Lys Val Tyr Thr Ile Asn 530 535 540 gcc aat gac gta gcc agg gat gcg ggc atg ggc cgc cgc acc aac aca 1680 Ala Asn Asp Val Ala Arg Asp Ala Gly Met Gly Arg Arg Thr Asn Thr 545 550 555 560 gtc atg caa acc tgt ttc ttt gcc cta gcg gga gtg tta ccc cgg gaa 1728 Val Met Gln Thr Cys Phe Phe Ala Leu Ala Gly Val Leu Pro Arg Glu 565 570 575 gag gcg atc gcc aaa att aag cag tcg gtc caa aaa acc tac ggc aaa 1776 Glu Ala Ile Ala Lys Ile Lys Gln Ser Val Gln Lys Thr Tyr Gly Lys 580 585 590 aag ggt cag gaa att gtc gag atg aat att aaa gcg gtg gat tcc acc 1824 Lys Gly Gln Glu Ile Val Glu Met Asn Ile Lys Ala Val Asp Ser Thr 595 600 605 ctg gcc cat ctc tat gaa gtg tcc gta ccg gaa acg gtg agc gac gat 1872 Leu Ala His Leu Tyr Glu Val Ser Val Pro Glu Thr Val Ser Asp Asp 610 615 620 gcc cct gct atg cgg ccg gtg gtg cct gat aac gcc ccg gtg ttt gtg 1920 Ala Pro Ala Met Arg Pro Val Val Pro Asp Asn Ala Pro Val Phe Val 625 630 635 640 cgg gaa gtg tta gga aaa atc atg gcc cgg caa ggg gat gat ctc ccg 1968 Arg Glu Val Leu Gly Lys Ile Met Ala Arg Gln Gly Asp Asp Leu Pro 645 650 655 gtc agt gct tta ccc tgc gat ggc acc tat ccc acc gcc act acc caa 2016 Val Ser Ala Leu Pro Cys Asp Gly Thr Tyr Pro Thr Ala Thr Thr Gln 660 665 670 tgg gaa aaa cgc aac gtg ggc cac gaa att ccc gtt tgg gac ccc gat 2064 Trp Glu Lys Arg Asn Val Gly His Glu Ile Pro Val Trp Asp Pro Asp 675 680 685 gtt tgt gtg caa tgc ggc aaa tgc gtc att gtt tgt ccc cat gct gtg 2112 Val Cys Val Gln Cys Gly Lys Cys Val Ile Val Cys Pro His Ala Val 690 695 700 att cgg ggc aaa gtt tac gag gag gca gaa ttg gcc aat gct ccg gtc 2160 Ile Arg Gly Lys Val Tyr Glu Glu Ala Glu Leu Ala Asn Ala Pro Val 705 710 715 720 agt ttc aaa ttt acc aat gcc aaa gac cat gat tgg caa ggt tct aag 2208 Ser Phe Lys Phe Thr Asn Ala Lys Asp His Asp Trp Gln Gly Ser Lys 725 730 735 ttc acc atc cag gta gcc ccg gaa gat tgc acc ggt tgc ggc atc tgt 2256 Phe Thr Ile Gln Val Ala Pro Glu Asp Cys Thr Gly Cys Gly Ile Cys 740 745 750 gtg gac gta tgc ccg gct aaa aat aaa tcc cag cct cgt tta agg gcg 2304 Val Asp Val Cys Pro Ala Lys Asn Lys Ser Gln Pro Arg Leu Arg Ala 755 760 765 att aat atg gct ccc cag tta ccc ttg cgg gaa cag gaa cgg gag aat 2352 Ile Asn Met Ala Pro Gln Leu Pro Leu Arg Glu Gln Glu Arg Glu Asn 770 775 780 tgg gac ttt ttc cta gat ttg ccc aac ccc gat cgc ctc agt ttg aat 2400 Trp Asp Phe Phe Leu Asp Leu Pro Asn Pro Asp Arg Leu Ser Leu Asn 785 790 795 800 ttg aac aaa atc agc cat caa cag atg cag gag ccg tta ttt gaa ttt 2448 Leu Asn Lys Ile Ser His Gln Gln Met Gln Glu Pro Leu Phe Glu Phe 805 810 815 tct gga gcc tgt gcc ggt tgt ggg gaa acc cct tat ttg aaa ctg gtc 2496 Ser Gly Ala Cys Ala Gly Cys Gly Glu Thr Pro Tyr Leu Lys Leu Val 820 825 830 agt caa tta ttt ggc gat cgc atg tta gtg gcc aac gcc acc ggt tgc 2544 Ser Gln Leu Phe Gly Asp Arg Met Leu Val Ala Asn Ala Thr Gly Cys 835 840 845 tct tcc atc tat ggc ggc aac tta ccg aca act ccc tgg gcc caa aat 2592 Ser Ser Ile Tyr Gly Gly Asn Leu Pro Thr Thr Pro Trp Ala Gln Asn 850 855 860 gct gag ggt cgc ggt ccc gct tgg tcc aat tcc ctg ttt gaa gat aac 2640 Ala Glu Gly Arg Gly Pro Ala Trp Ser Asn Ser Leu Phe Glu Asp Asn 865 870 875 880 gct gaa ttt ggc ctt ggt ttc cga gtg gcg atc gac aag caa acg gaa 2688 Ala Glu Phe Gly Leu Gly Phe Arg Val Ala Ile Asp Lys Gln Thr Glu 885 890 895 ttt gca ggg gaa ttg cta aaa acc ttt gct ggg gag ttg gga gac agt 2736 Phe Ala Gly Glu Leu Leu Lys Thr Phe Ala Gly Glu Leu Gly Asp Ser 900 905 910 ttg gta agt gaa att ctc aac aat gcc caa acc act gaa gcg gat att 2784 Leu Val Ser Glu Ile Leu Asn Asn Ala Gln Thr Thr Glu Ala Asp Ile 915 920 925 ttt gaa caa cgg caa ttg gta gaa cag gtt aag caa cgt ttg caa aat 2832 Phe Glu Gln Arg Gln Leu Val Glu Gln Val Lys Gln Arg Leu Gln Asn 930 935 940 ctg gaa act ccc caa gcc caa atg ttc ctt tct gta gcg gat tac ctc 2880 Leu Glu Thr Pro Gln Ala Gln Met Phe Leu Ser Val Ala Asp Tyr Leu 945 950 955 960 gtg aag aaa agc gtt tgg att att ggt ggc gat ggc tgg gcc tac gac 2928 Val Lys Lys Ser Val Trp Ile Ile Gly Gly Asp Gly Trp Ala Tyr Asp 965 970 975 att ggg tac ggc ggt ttg gat cac gtc ctc gcc agt ggg cgt aat gtc 2976 Ile Gly Tyr Gly Gly Leu Asp His Val Leu Ala Ser Gly Arg Asn Val 980 985 990 aat atc ttg gtg atg gat acg gaa gtc tat tcc aac acc ggg ggc caa 3024 Asn Ile Leu Val Met Asp Thr Glu Val Tyr Ser Asn Thr Gly Gly Gln 995 1000 1005 gcc tcc aaa gcc act ccc cgg gcc gct gta gct aaa ttc gcc gct 3069 Ala Ser Lys Ala Thr Pro Arg Ala Ala Val Ala Lys Phe Ala Ala 1010 1015 1020 ggg ggt aaa ccc tct ccc aaa aaa gat ttg ggc tta atg gcc atg 3114 Gly Gly Lys Pro Ser Pro Lys Lys Asp Leu Gly Leu Met Ala Met 1025 1030 1035 acc tac ggc aac gtc tat gtg gcc agt atc gcc atg gga gcc aaa 3159 Thr Tyr Gly Asn Val Tyr Val Ala Ser Ile Ala Met Gly Ala Lys 1040 1045 1050 aat gag cag tcc att aaa gcc ttt atg gaa gcg gaa gcc tat ccc 3204 Asn Glu Gln Ser Ile Lys Ala Phe Met Glu Ala Glu Ala Tyr Pro 1055 1060 1065 ggt gtc tcg tta att att gcc tac tcc cac tgc att gcc cac ggc 3249 Gly Val Ser Leu Ile Ile Ala Tyr Ser His Cys Ile Ala His Gly 1070 1075 1080 att aat atg acc acc gcg atg aac cat caa aaa gag ttg gtg gac 3294 Ile Asn Met Thr Thr Ala Met Asn His Gln Lys Glu Leu Val Asp 1085 1090 1095 agc ggt cgt tgg ttg ctc tac cgc tat aac cct ttg ttg gcg gat 3339 Ser Gly Arg Trp Leu Leu Tyr Arg Tyr Asn Pro Leu Leu Ala Asp 1100 1105 1110 gaa ggt aaa aat ccc ctg caa ttg gat atg gga tcg cca aaa gta 3384 Glu Gly Lys Asn Pro Leu Gln Leu Asp Met Gly Ser Pro Lys Val 1115 1120 1125 gcc att gac aaa acg gtc tat tcg gaa aat cgc ttt gcc atg ctc 3429 Ala Ile Asp Lys Thr Val Tyr Ser Glu Asn Arg Phe Ala Met Leu 1130 1135 1140 acc cgc agt caa cca gag gag gcc aaa cgc tta atg aag tta gct 3474 Thr Arg Ser Gln Pro Glu Glu Ala Lys Arg Leu Met Lys Leu Ala 1145 1150 1155 caa ggg gat gtg aac act cgc tgg gcc atg tac gaa tat ctg gcg 3519 Gln Gly Asp Val Asn Thr Arg Trp Ala Met Tyr Glu Tyr Leu Ala 1160 1165 1170 aaa cgt tct ctg ggt ggg gaa att aac ggt aac aac cat ggt gtt 3564 Lys Arg Ser Leu Gly Gly Glu Ile Asn Gly Asn Asn His Gly Val 1175 1180 1185 tcc cca tct ccg gag gta att gct aaa tct gtt tag 3600 Ser Pro Ser Pro Glu Val Ile Ala Lys Ser Val 1190 1195 <210> 261 <211> 1199 <212> PRT <213> Synechocystis PCC6803 <400> 261 Met Ser Leu Pro Thr Tyr Ala Thr Leu Asp Gly Asn Glu Ala Val Ala 1 5 10 15 Arg Val Ala Tyr Leu Leu Ser Glu Val Ile Ala Ile Tyr Pro Ile Thr 20 25 30 Pro Ser Ser Pro Met Gly Glu Trp Ser Asp Ala Trp Ala Ala Glu His 35 40 45 Arg Pro Asn Leu Trp Gly Thr Val Pro Leu Val Val Glu Met Gln Ser 50 55 60 Glu Gly Gly Ala Ala Gly Thr Val His Gly Ala Leu Gln Ser Gly Ala 65 70 75 80 Leu Thr Thr Thr Phe Thr Ala Ser Gln Gly Leu Met Leu Met Leu Pro 85 90 95 Asn Met His Lys Ile Ala Gly Glu Leu Thr Ala Met Val Leu His Val 100 105 110 Ala Ala Arg Ser Leu Ala Ala Gln Gly Leu Ser Ile Phe Gly Asp His 115 120 125 Ser Asp Val Met Ala Ala Arg Asn Thr Gly Phe Ala Met Leu Ser Ser 130 135 140 Asn Ser Val Gln Glu Ala His Asp Phe Ala Leu Ile Ala Thr Ala Thr 145 150 155 160 Ser Phe Ala Thr Arg Ile Pro Gly Leu His Phe Phe Asp Gly Phe Arg 165 170 175 Thr Ser His Glu Glu Gln Lys Ile Glu Leu Leu Pro Gln Glu Val Leu 180 185 190 Arg Gly Leu Ile Lys Asp Glu Asp Val Leu Ala His Arg Gly Arg Ala 195 200 205 Leu Thr Pro Asp Arg Pro Lys Leu Arg Gly Thr Ala Gln Asn Pro Asp 210 215 220 Val Tyr Phe Gln Ala Arg Glu Thr Val Asn Pro Phe Tyr Ala Ser Tyr 225 230 235 240 Pro Asn Val Leu Glu Gln Val Met Glu Gln Phe Gly Gln Leu Thr Gly 245 250 255 Arg His Tyr Arg Pro Tyr Glu Tyr Cys Gly His Pro Glu Ala Glu Arg 260 265 270 Val Ile Val Leu Met Gly Ser Gly Ala Glu Thr Ala Gln Glu Thr Val 275 280 285 Asp Phe Leu Thr Ala Gln Gly Glu Lys Val Gly Leu Leu Lys Val Arg 290 295 300 Leu Tyr Arg Pro Phe Ala Gly Asp Arg Leu Val Asn Ala Leu Pro Lys 305 310 315 320 Thr Val Gln Lys Ile Ala Val Leu Asp Arg Cys Lys Glu Pro Gly Ser 325 330 335 Ile Gly Glu Pro Leu Tyr Gln Asp Val Leu Thr Ala Phe Phe Glu Ala 340 345 350 Gly Met Met Pro Lys Ile Ile Gly Gly Arg Tyr Gly Leu Ser Ser Lys 355 360 365 Glu Phe Thr Pro Ala Met Val Lys Gly Val Leu Asp His Leu Asn Gln 370 375 380 Thr Asn Pro Lys Asn His Phe Thr Val Gly Ile Asn Asp Asp Leu Ser 385 390 395 400 His Thr Ser Ile Asp Tyr Asp Pro Ser Phe Ser Thr Glu Ala Asp Ser 405 410 415 Val Val Arg Ala Ile Phe Tyr Gly Leu Gly Ser Asp Gly Thr Val Gly 420 425 430 Ala Asn Lys Asn Ser Ile Lys Ile Ile Gly Glu Asp Thr Asp Asn Tyr 435 440 445 Ala Gln Gly Tyr Phe Val Tyr Asp Ser Lys Lys Ser Gly Ser Val Thr 450 455 460 Val Ser His Leu Arg Phe Gly Pro Asn Pro Ile Leu Ser Thr Tyr Leu 465 470 475 480 Ile Ser Gln Ala Asn Phe Val Ala Cys His Gln Trp Glu Phe Leu Glu 485 490 495 Gln Phe Glu Val Leu Glu Pro Ala Val Asp Gly Gly Val Phe Leu Val 500 505 510 Asn Ser Pro Tyr Gly Pro Glu Glu Ile Trp Arg Glu Phe Pro Arg Lys 515 520 525 Val Gln Gln Glu Ile Ile Asp Lys Asn Leu Lys Val Tyr Thr Ile Asn 530 535 540 Ala Asn Asp Val Ala Arg Asp Ala Gly Met Gly Arg Arg Thr Asn Thr 545 550 555 560 Val Met Gln Thr Cys Phe Phe Ala Leu Ala Gly Val Leu Pro Arg Glu 565 570 575 Glu Ala Ile Ala Lys Ile Lys Gln Ser Val Gln Lys Thr Tyr Gly Lys 580 585 590 Lys Gly Gln Glu Ile Val Glu Met Asn Ile Lys Ala Val Asp Ser Thr 595 600 605 Leu Ala His Leu Tyr Glu Val Ser Val Pro Glu Thr Val Ser Asp Asp 610 615 620 Ala Pro Ala Met Arg Pro Val Val Pro Asp Asn Ala Pro Val Phe Val 625 630 635 640 Arg Glu Val Leu Gly Lys Ile Met Ala Arg Gln Gly Asp Asp Leu Pro 645 650 655 Val Ser Ala Leu Pro Cys Asp Gly Thr Tyr Pro Thr Ala Thr Thr Gln 660 665 670 Trp Glu Lys Arg Asn Val Gly His Glu Ile Pro Val Trp Asp Pro Asp 675 680 685 Val Cys Val Gln Cys Gly Lys Cys Val Ile Val Cys Pro His Ala Val 690 695 700 Ile Arg Gly Lys Val Tyr Glu Glu Ala Glu Leu Ala Asn Ala Pro Val 705 710 715 720 Ser Phe Lys Phe Thr Asn Ala Lys Asp His Asp Trp Gln Gly Ser Lys 725 730 735 Phe Thr Ile Gln Val Ala Pro Glu Asp Cys Thr Gly Cys Gly Ile Cys 740 745 750 Val Asp Val Cys Pro Ala Lys Asn Lys Ser Gln Pro Arg Leu Arg Ala 755 760 765 Ile Asn Met Ala Pro Gln Leu Pro Leu Arg Glu Gln Glu Arg Glu Asn 770 775 780 Trp Asp Phe Phe Leu Asp Leu Pro Asn Pro Asp Arg Leu Ser Leu Asn 785 790 795 800 Leu Asn Lys Ile Ser His Gln Gln Met Gln Glu Pro Leu Phe Glu Phe 805 810 815 Ser Gly Ala Cys Ala Gly Cys Gly Glu Thr Pro Tyr Leu Lys Leu Val 820 825 830 Ser Gln Leu Phe Gly Asp Arg Met Leu Val Ala Asn Ala Thr Gly Cys 835 840 845 Ser Ser Ile Tyr Gly Gly Asn Leu Pro Thr Thr Pro Trp Ala Gln Asn 850 855 860 Ala Glu Gly Arg Gly Pro Ala Trp Ser Asn Ser Leu Phe Glu Asp Asn 865 870 875 880 Ala Glu Phe Gly Leu Gly Phe Arg Val Ala Ile Asp Lys Gln Thr Glu 885 890 895 Phe Ala Gly Glu Leu Leu Lys Thr Phe Ala Gly Glu Leu Gly Asp Ser 900 905 910 Leu Val Ser Glu Ile Leu Asn Asn Ala Gln Thr Thr Glu Ala Asp Ile 915 920 925 Phe Glu Gln Arg Gln Leu Val Glu Gln Val Lys Gln Arg Leu Gln Asn 930 935 940 Leu Glu Thr Pro Gln Ala Gln Met Phe Leu Ser Val Ala Asp Tyr Leu 945 950 955 960 Val Lys Lys Ser Val Trp Ile Ile Gly Gly Asp Gly Trp Ala Tyr Asp 965 970 975 Ile Gly Tyr Gly Gly Leu Asp His Val Leu Ala Ser Gly Arg Asn Val 980 985 990 Asn Ile Leu Val Met Asp Thr Glu Val Tyr Ser Asn Thr Gly Gly Gln 995 1000 1005 Ala Ser Lys Ala Thr Pro Arg Ala Ala Val Ala Lys Phe Ala Ala 1010 1015 1020 Gly Gly Lys Pro Ser Pro Lys Lys Asp Leu Gly Leu Met Ala Met 1025 1030 1035 Thr Tyr Gly Asn Val Tyr Val Ala Ser Ile Ala Met Gly Ala Lys 1040 1045 1050 Asn Glu Gln Ser Ile Lys Ala Phe Met Glu Ala Glu Ala Tyr Pro 1055 1060 1065 Gly Val Ser Leu Ile Ile Ala Tyr Ser His Cys Ile Ala His Gly 1070 1075 1080 Ile Asn Met Thr Thr Ala Met Asn His Gln Lys Glu Leu Val Asp 1085 1090 1095 Ser Gly Arg Trp Leu Leu Tyr Arg Tyr Asn Pro Leu Leu Ala Asp 1100 1105 1110 Glu Gly Lys Asn Pro Leu Gln Leu Asp Met Gly Ser Pro Lys Val 1115 1120 1125 Ala Ile Asp Lys Thr Val Tyr Ser Glu Asn Arg Phe Ala Met Leu 1130 1135 1140 Thr Arg Ser Gln Pro Glu Glu Ala Lys Arg Leu Met Lys Leu Ala 1145 1150 1155 Gln Gly Asp Val Asn Thr Arg Trp Ala Met Tyr Glu Tyr Leu Ala 1160 1165 1170 Lys Arg Ser Leu Gly Gly Glu Ile Asn Gly Asn Asn His Gly Val 1175 1180 1185 Ser Pro Ser Pro Glu Val Ile Ala Lys Ser Val 1190 1195 <210> 262 <211> 531 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(531) <223> fldA gene from E. coli encoding flavodoxin <400> 262 atg gct atc act ggc atc ttt ttc ggc agc gac acc ggt aat acc gaa 48 Met Ala Ile Thr Gly Ile Phe Phe Gly Ser Asp Thr Gly Asn Thr Glu 1 5 10 15 aat atc gca aaa atg att caa aaa cag ctt ggt aaa gac gtt gcc gat 96 Asn Ile Ala Lys Met Ile Gln Lys Gln Leu Gly Lys Asp Val Ala Asp 20 25 30 gtc cat gac att gca aaa agc agc aaa gaa gat ctg gaa gct tat gac 144 Val His Asp Ile Ala Lys Ser Ser Lys Glu Asp Leu Glu Ala Tyr Asp 35 40 45 att ctg ctg ctg ggc atc cca acc tgg tat tac ggc gaa gcg cag tgt 192 Ile Leu Leu Leu Gly Ile Pro Thr Trp Tyr Tyr Gly Glu Ala Gln Cys 50 55 60 gac tgg gat gac ttc ttc ccg act ctc gaa gag att gat ttc aac ggc 240 Asp Trp Asp Asp Phe Phe Pro Thr Leu Glu Glu Ile Asp Phe Asn Gly 65 70 75 80 aaa ctg gtt gcg ctg ttt ggt tgt ggt gac cag gaa gat tac gcc gaa 288 Lys Leu Val Ala Leu Phe Gly Cys Gly Asp Gln Glu Asp Tyr Ala Glu 85 90 95 tat ttc tgc gac gca ttg ggc acc atc cgc gac atc att gaa ccg cgc 336 Tyr Phe Cys Asp Ala Leu Gly Thr Ile Arg Asp Ile Ile Glu Pro Arg 100 105 110 ggt gca acc atc gtt ggt cac tgg cca act gcg ggc tat cat ttc gaa 384 Gly Ala Thr Ile Val Gly His Trp Pro Thr Ala Gly Tyr His Phe Glu 115 120 125 gca tca aaa ggt ctg gca gat gac gac cac ttt gtc ggt ctg gct atc 432 Ala Ser Lys Gly Leu Ala Asp Asp Asp His Phe Val Gly Leu Ala Ile 130 135 140 gac gaa gac cgt cag ccg gaa ctg acc gct gaa cgt gta gaa aaa tgg 480 Asp Glu Asp Arg Gln Pro Glu Leu Thr Ala Glu Arg Val Glu Lys Trp 145 150 155 160 gtt aaa cag att tct gaa gag ttg cat ctc gac gaa att ctc aat gcc 528 Val Lys Gln Ile Ser Glu Glu Leu His Leu Asp Glu Ile Leu Asn Ala 165 170 175 tga 531 <210> 263 <211> 176 <212> PRT <213> Escherichia coli <400> 263 Met Ala Ile Thr Gly Ile Phe Phe Gly Ser Asp Thr Gly Asn Thr Glu 1 5 10 15 Asn Ile Ala Lys Met Ile Gln Lys Gln Leu Gly Lys Asp Val Ala Asp 20 25 30 Val His Asp Ile Ala Lys Ser Ser Lys Glu Asp Leu Glu Ala Tyr Asp 35 40 45 Ile Leu Leu Leu Gly Ile Pro Thr Trp Tyr Tyr Gly Glu Ala Gln Cys 50 55 60 Asp Trp Asp Asp Phe Phe Pro Thr Leu Glu Glu Ile Asp Phe Asn Gly 65 70 75 80 Lys Leu Val Ala Leu Phe Gly Cys Gly Asp Gln Glu Asp Tyr Ala Glu 85 90 95 Tyr Phe Cys Asp Ala Leu Gly Thr Ile Arg Asp Ile Ile Glu Pro Arg 100 105 110 Gly Ala Thr Ile Val Gly His Trp Pro Thr Ala Gly Tyr His Phe Glu 115 120 125 Ala Ser Lys Gly Leu Ala Asp Asp Asp His Phe Val Gly Leu Ala Ile 130 135 140 Asp Glu Asp Arg Gln Pro Glu Leu Thr Ala Glu Arg Val Glu Lys Trp 145 150 155 160 Val Lys Gln Ile Ser Glu Glu Leu His Leu Asp Glu Ile Leu Asn Ala 165 170 175 <210> 264 <211> 522 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(522) <223> fldB gene from E. coli encoding flavodoxin <400> 264 atg aat atg ggt ctt ttt tac ggt tcc agc acc tgt tac acc gaa atg 48 Met Asn Met Gly Leu Phe Tyr Gly Ser Ser Thr Cys Tyr Thr Glu Met 1 5 10 15 gcg gca gaa aaa atc cgc gat att atc ggc cca gaa ctg gtg acc tta 96 Ala Ala Glu Lys Ile Arg Asp Ile Ile Gly Pro Glu Leu Val Thr Leu 20 25 30 cat aac ctc aag gac gac tcc ccg aaa tta atg gag cag tac gat gtg 144 His Asn Leu Lys Asp Asp Ser Pro Lys Leu Met Glu Gln Tyr Asp Val 35 40 45 ctc att ctg ggt atc ccg acc tgg gat ttt ggt gaa atc cag gaa gac 192 Leu Ile Leu Gly Ile Pro Thr Trp Asp Phe Gly Glu Ile Gln Glu Asp 50 55 60 tgg gaa gcc gtc tgg gat cag ctc gac gac ctg aac ctt gaa ggt aaa 240 Trp Glu Ala Val Trp Asp Gln Leu Asp Asp Leu Asn Leu Glu Gly Lys 65 70 75 80 att gtt gcg ctg tat ggg ctt ggc gat caa ctg gga tac ggc gag tgg 288 Ile Val Ala Leu Tyr Gly Leu Gly Asp Gln Leu Gly Tyr Gly Glu Trp 85 90 95 ttc ctc gat gcg ctc ggt atg ctg cat gac aaa ctc tcg acc aaa ggc 336 Phe Leu Asp Ala Leu Gly Met Leu His Asp Lys Leu Ser Thr Lys Gly 100 105 110 gtg aag ttc gtc ggc tac tgg cca acg gaa gga tat gaa ttt acc agc 384 Val Lys Phe Val Gly Tyr Trp Pro Thr Glu Gly Tyr Glu Phe Thr Ser 115 120 125 ccg aaa ccg gtg att gct gac ggg caa ctg ttc gtg ggt ctg gcg ctg 432 Pro Lys Pro Val Ile Ala Asp Gly Gln Leu Phe Val Gly Leu Ala Leu 130 135 140 gat gaa act aac cag tat gac ctt agc gac gag cgt att cag agc tgg 480 Asp Glu Thr Asn Gln Tyr Asp Leu Ser Asp Glu Arg Ile Gln Ser Trp 145 150 155 160 tgc gag caa atc ctc aac gaa atg gca gag cat tac gcc tga 522 Cys Glu Gln Ile Leu Asn Glu Met Ala Glu His Tyr Ala 165 170 <210> 265 <211> 173 <212> PRT <213> Escherichia coli <400> 265 Met Asn Met Gly Leu Phe Tyr Gly Ser Ser Thr Cys Tyr Thr Glu Met 1 5 10 15 Ala Ala Glu Lys Ile Arg Asp Ile Ile Gly Pro Glu Leu Val Thr Leu 20 25 30 His Asn Leu Lys Asp Asp Ser Pro Lys Leu Met Glu Gln Tyr Asp Val 35 40 45 Leu Ile Leu Gly Ile Pro Thr Trp Asp Phe Gly Glu Ile Gln Glu Asp 50 55 60 Trp Glu Ala Val Trp Asp Gln Leu Asp Asp Leu Asn Leu Glu Gly Lys 65 70 75 80 Ile Val Ala Leu Tyr Gly Leu Gly Asp Gln Leu Gly Tyr Gly Glu Trp 85 90 95 Phe Leu Asp Ala Leu Gly Met Leu His Asp Lys Leu Ser Thr Lys Gly 100 105 110 Val Lys Phe Val Gly Tyr Trp Pro Thr Glu Gly Tyr Glu Phe Thr Ser 115 120 125 Pro Lys Pro Val Ile Ala Asp Gly Gln Leu Phe Val Gly Leu Ala Leu 130 135 140 Asp Glu Thr Asn Gln Tyr Asp Leu Ser Asp Glu Arg Ile Gln Ser Trp 145 150 155 160 Cys Glu Gln Ile Leu Asn Glu Met Ala Glu His Tyr Ala 165 170 <210> 266 <211> 477 <212> DNA <213> Bacillus subtilis <220> <221> CDS <222> (1)..(477) <223> ykuN gene from Bacillus subtilis encoding flavodoxin <400> 266 atg gct aaa gcc ttg att aca tat gcc agc atg tca gga aat aca gaa 48 Met Ala Lys Ala Leu Ile Thr Tyr Ala Ser Met Ser Gly Asn Thr Glu 1 5 10 15 gac att gcc ttc ata ata aaa gat acg ctt cag gaa tat gag ttg gat 96 Asp Ile Ala Phe Ile Ile Lys Asp Thr Leu Gln Glu Tyr Glu Leu Asp 20 25 30 atc gat tgt gtc gag ata aat gat atg gat gcg tct tgt tta acc tcc 144 Ile Asp Cys Val Glu Ile Asn Asp Met Asp Ala Ser Cys Leu Thr Ser 35 40 45 tat gat tat gta ctg att ggc acc tat aca tgg ggg gac ggc gat ttg 192 Tyr Asp Tyr Val Leu Ile Gly Thr Tyr Thr Trp Gly Asp Gly Asp Leu 50 55 60 ccc tac gaa gcg gag gat ttt ttc gaa gag gtc aaa cag att cag ctt 240 Pro Tyr Glu Ala Glu Asp Phe Phe Glu Glu Val Lys Gln Ile Gln Leu 65 70 75 80 aat ggt tta aaa aca gcc tgc ttc ggg tct ggc gat tat tct tat cca 288 Asn Gly Leu Lys Thr Ala Cys Phe Gly Ser Gly Asp Tyr Ser Tyr Pro 85 90 95 aag ttt tgc gaa gcg gtg aat ttg ttc aat gtc atg ctg caa gag gcg 336 Lys Phe Cys Glu Ala Val Asn Leu Phe Asn Val Met Leu Gln Glu Ala 100 105 110 gga gct gct gtt tac cag gaa aca cta aaa att gaa tta gcg cct gaa 384 Gly Ala Ala Val Tyr Gln Glu Thr Leu Lys Ile Glu Leu Ala Pro Glu 115 120 125 aca gat gaa gat gtg gaa agc tgc cga gcg ttt gcg aga ggt ttt ctt 432 Thr Asp Glu Asp Val Glu Ser Cys Arg Ala Phe Ala Arg Gly Phe Leu 130 135 140 gca tgg gca gat tat atg aac aag gaa aaa atc cat gtt tca taa 477 Ala Trp Ala Asp Tyr Met Asn Lys Glu Lys Ile His Val Ser 145 150 155 <210> 267 <211> 158 <212> PRT <213> Bacillus subtilis <400> 267 Met Ala Lys Ala Leu Ile Thr Tyr Ala Ser Met Ser Gly Asn Thr Glu 1 5 10 15 Asp Ile Ala Phe Ile Ile Lys Asp Thr Leu Gln Glu Tyr Glu Leu Asp 20 25 30 Ile Asp Cys Val Glu Ile Asn Asp Met Asp Ala Ser Cys Leu Thr Ser 35 40 45 Tyr Asp Tyr Val Leu Ile Gly Thr Tyr Thr Trp Gly Asp Gly Asp Leu 50 55 60 Pro Tyr Glu Ala Glu Asp Phe Phe Glu Glu Val Lys Gln Ile Gln Leu 65 70 75 80 Asn Gly Leu Lys Thr Ala Cys Phe Gly Ser Gly Asp Tyr Ser Tyr Pro 85 90 95 Lys Phe Cys Glu Ala Val Asn Leu Phe Asn Val Met Leu Gln Glu Ala 100 105 110 Gly Ala Ala Val Tyr Gln Glu Thr Leu Lys Ile Glu Leu Ala Pro Glu 115 120 125 Thr Asp Glu Asp Val Glu Ser Cys Arg Ala Phe Ala Arg Gly Phe Leu 130 135 140 Ala Trp Ala Asp Tyr Met Asn Lys Glu Lys Ile His Val Ser 145 150 155 <210> 268 <211> 513 <212> DNA <213> Synechocystis PCC6803 <220> <221> CDS <222> (1)..(513) <223> isiB gene from Synechocystis encoding flavodoxin <400> 268 atg aca aaa att gga ctt ttt tac ggt act caa acc ggc aac act gaa 48 Met Thr Lys Ile Gly Leu Phe Tyr Gly Thr Gln Thr Gly Asn Thr Glu 1 5 10 15 acc att gct gaa ctg att caa aaa gaa atg ggc ggc gat agt gtg gtc 96 Thr Ile Ala Glu Leu Ile Gln Lys Glu Met Gly Gly Asp Ser Val Val 20 25 30 gat atg atg gat ata tcc cag gct gat gtt gat gat ttt agg caa tat 144 Asp Met Met Asp Ile Ser Gln Ala Asp Val Asp Asp Phe Arg Gln Tyr 35 40 45 agt tgc ctg att atc ggt tgt ccc acc tgg aat gtg ggg gaa ctc cag 192 Ser Cys Leu Ile Ile Gly Cys Pro Thr Trp Asn Val Gly Glu Leu Gln 50 55 60 agt gat tgg gaa ggc ttt tat gac caa tta gac gaa att gat ttt aat 240 Ser Asp Trp Glu Gly Phe Tyr Asp Gln Leu Asp Glu Ile Asp Phe Asn 65 70 75 80 ggc aaa aaa gta gcc tat ttt ggt gct ggc gat cag gtt ggt tat gca 288 Gly Lys Lys Val Ala Tyr Phe Gly Ala Gly Asp Gln Val Gly Tyr Ala 85 90 95 gat aat ttt caa gac gcc atg ggc att tta gaa gaa aaa atc agt gga 336 Asp Asn Phe Gln Asp Ala Met Gly Ile Leu Glu Glu Lys Ile Ser Gly 100 105 110 tta ggc ggt aaa aca gtg ggg ttt tgg ccc acc gct ggc tat gat ttt 384 Leu Gly Gly Lys Thr Val Gly Phe Trp Pro Thr Ala Gly Tyr Asp Phe 115 120 125 gac gaa tca aaa gcg gtg aaa aat ggg aaa ttt gtt ggt tta gct ttg 432 Asp Glu Ser Lys Ala Val Lys Asn Gly Lys Phe Val Gly Leu Ala Leu 130 135 140 gac gaa gat aat cag cca gag tta aca gaa tta aga gta aag aca tgg 480 Asp Glu Asp Asn Gln Pro Glu Leu Thr Glu Leu Arg Val Lys Thr Trp 145 150 155 160 gta agt gaa att aaa cca att ttg caa tcc tag 513 Val Ser Glu Ile Lys Pro Ile Leu Gln Ser 165 170 <210> 269 <211> 170 <212> PRT <213> Synechocystis PCC6803 <400> 269 Met Thr Lys Ile Gly Leu Phe Tyr Gly Thr Gln Thr Gly Asn Thr Glu 1 5 10 15 Thr Ile Ala Glu Leu Ile Gln Lys Glu Met Gly Gly Asp Ser Val Val 20 25 30 Asp Met Met Asp Ile Ser Gln Ala Asp Val Asp Asp Phe Arg Gln Tyr 35 40 45 Ser Cys Leu Ile Ile Gly Cys Pro Thr Trp Asn Val Gly Glu Leu Gln 50 55 60 Ser Asp Trp Glu Gly Phe Tyr Asp Gln Leu Asp Glu Ile Asp Phe Asn 65 70 75 80 Gly Lys Lys Val Ala Tyr Phe Gly Ala Gly Asp Gln Val Gly Tyr Ala 85 90 95 Asp Asn Phe Gln Asp Ala Met Gly Ile Leu Glu Glu Lys Ile Ser Gly 100 105 110 Leu Gly Gly Lys Thr Val Gly Phe Trp Pro Thr Ala Gly Tyr Asp Phe 115 120 125 Asp Glu Ser Lys Ala Val Lys Asn Gly Lys Phe Val Gly Leu Ala Leu 130 135 140 Asp Glu Asp Asn Gln Pro Glu Leu Thr Glu Leu Arg Val Lys Thr Trp 145 150 155 160 Val Ser Glu Ile Lys Pro Ile Leu Gln Ser 165 170 <210> 270 <211> 585 <212> DNA <213> Streptomyces venezuelae <220> <221> CDS <222> (1)..(585) <223> wrbA gene from Streptomyces venezuelae encoding flavodoxin <400> 270 atg acc acc ccc gtc gtc tcc atc gcc tac cac tcc ggc tac ggc cac 48 Met Thr Thr Pro Val Val Ser Ile Ala Tyr His Ser Gly Tyr Gly His 1 5 10 15 acc gcg gtc ctg gcc gag gcc gtc cgt gac ggc gcc gcc gac gcg ggc 96 Thr Ala Val Leu Ala Glu Ala Val Arg Asp Gly Ala Ala Asp Ala Gly 20 25 30 gcc acc gtc cac ctg atc aag gtc gac ggg atc acc gag gcg gag tgg 144 Ala Thr Val His Leu Ile Lys Val Asp Gly Ile Thr Glu Ala Glu Trp 35 40 45 gag ctg ctc gac gcc tcc gac gcg atc gtc ttc ggc tcc ccg acc tac 192 Glu Leu Leu Asp Ala Ser Asp Ala Ile Val Phe Gly Ser Pro Thr Tyr 50 55 60 atg ggc acc gcc tcc ggt gcc ttc cac cag ttc gcc gag gac tcc tcg 240 Met Gly Thr Ala Ser Gly Ala Phe His Gln Phe Ala Glu Asp Ser Ser 65 70 75 80 aag cgc tgg ttc ggc gac gtc tgg ctg gac aag ctc gcc gcc ggc ttc 288 Lys Arg Trp Phe Gly Asp Val Trp Leu Asp Lys Leu Ala Ala Gly Phe 85 90 95 acc aac tcc ggc tcc aag agc ggc gac aag ctg cac acc ctg cag tac 336 Thr Asn Ser Gly Ser Lys Ser Gly Asp Lys Leu His Thr Leu Gln Tyr 100 105 110 ttc cag atc ctc gcc ggc cag cac ggc atg cac tgg gtc aac ctc ggc 384 Phe Gln Ile Leu Ala Gly Gln His Gly Met His Trp Val Asn Leu Gly 115 120 125 ctg aag ccc ggc tgg aac acc agc gag gcc tcc gag aac gac atc aac 432 Leu Lys Pro Gly Trp Asn Thr Ser Glu Ala Ser Glu Asn Asp Ile Asn 130 135 140 cgc ctc ggc ttc ttc tcc ggc gcc gcc ggc cag acc ccc gcg gac ctg 480 Arg Leu Gly Phe Phe Ser Gly Ala Ala Gly Gln Thr Pro Ala Asp Leu 145 150 155 160 ggc ccc gag gcc gtc cac aag gcc gac gtc gcc acc gcc gaa cac ctc 528 Gly Pro Glu Ala Val His Lys Ala Asp Val Ala Thr Ala Glu His Leu 165 170 175 ggc cgc cgc gtc gcc gag acc gcc cgc acc ttc gcg gcc ggc aag gcc 576 Gly Arg Arg Val Ala Glu Thr Ala Arg Thr Phe Ala Ala Gly Lys Ala 180 185 190 gcc gcc tga 585 Ala Ala <210> 271 <211> 194 <212> PRT <213> Streptomyces venezuelae <400> 271 Met Thr Thr Pro Val Val Ser Ile Ala Tyr His Ser Gly Tyr Gly His 1 5 10 15 Thr Ala Val Leu Ala Glu Ala Val Arg Asp Gly Ala Ala Asp Ala Gly 20 25 30 Ala Thr Val His Leu Ile Lys Val Asp Gly Ile Thr Glu Ala Glu Trp 35 40 45 Glu Leu Leu Asp Ala Ser Asp Ala Ile Val Phe Gly Ser Pro Thr Tyr 50 55 60 Met Gly Thr Ala Ser Gly Ala Phe His Gln Phe Ala Glu Asp Ser Ser 65 70 75 80 Lys Arg Trp Phe Gly Asp Val Trp Leu Asp Lys Leu Ala Ala Gly Phe 85 90 95 Thr Asn Ser Gly Ser Lys Ser Gly Asp Lys Leu His Thr Leu Gln Tyr 100 105 110 Phe Gln Ile Leu Ala Gly Gln His Gly Met His Trp Val Asn Leu Gly 115 120 125 Leu Lys Pro Gly Trp Asn Thr Ser Glu Ala Ser Glu Asn Asp Ile Asn 130 135 140 Arg Leu Gly Phe Phe Ser Gly Ala Ala Gly Gln Thr Pro Ala Asp Leu 145 150 155 160 Gly Pro Glu Ala Val His Lys Ala Asp Val Ala Thr Ala Glu His Leu 165 170 175 Gly Arg Arg Val Ala Glu Thr Ala Arg Thr Phe Ala Ala Gly Lys Ala 180 185 190 Ala Ala <210> 272 <211> 459 <212> DNA <213> Methanococcus aeolicus <220> <221> CDS <222> (1)..(459) <223> PRK06242 gene from Methanococcus aeolicus encoding flavodoxin <400> 272 atg aaa ata tta att att tgt aaa tcc gta cac cat gga aac act aaa 48 Met Lys Ile Leu Ile Ile Cys Lys Ser Val His His Gly Asn Thr Lys 1 5 10 15 aaa ata gca gat gcc atg gca gag gtt tta aat gca gag gtt att gca 96 Lys Ile Ala Asp Ala Met Ala Glu Val Leu Asn Ala Glu Val Ile Ala 20 25 30 cct gaa aat gta agt tcc gaa gat atc aaa aaa tat gat ttg gtg gga 144 Pro Glu Asn Val Ser Ser Glu Asp Ile Lys Lys Tyr Asp Leu Val Gly 35 40 45 ttt ggc tct gga ata tat att ggg aaa cat cat aaa aag cta tta aaa 192 Phe Gly Ser Gly Ile Tyr Ile Gly Lys His His Lys Lys Leu Leu Lys 50 55 60 ctt gcg gat aat ctt cca aat gga gaa aat aaa aca gta ttt gta ttt 240 Leu Ala Asp Asn Leu Pro Asn Gly Glu Asn Lys Thr Val Phe Val Phe 65 70 75 80 tcc aca agc gat aac tgg aag caa aat tac cat aag cca tta atg gat 288 Ser Thr Ser Asp Asn Trp Lys Gln Asn Tyr His Lys Pro Leu Met Asp 85 90 95 aaa cta aat tcc aga gga tat aaa aca gta gga gaa ttc aac tgt aaa 336 Lys Leu Asn Ser Arg Gly Tyr Lys Thr Val Gly Glu Phe Asn Cys Lys 100 105 110 ggg ttt gat gac tgg ttt ata ttt aaa tta att ggt ggt aga aat aaa 384 Gly Phe Asp Asp Trp Phe Ile Phe Lys Leu Ile Gly Gly Arg Asn Lys 115 120 125 gga cat cca aat aaa aaa gat att gaa aat gca aaa aaa ttt gct gaa 432 Gly His Pro Asn Lys Lys Asp Ile Glu Asn Ala Lys Lys Phe Ala Glu 130 135 140 aat ata aag aat ata gaa aat ata tag 459 Asn Ile Lys Asn Ile Glu Asn Ile 145 150 <210> 273 <211> 152 <212> PRT <213> Methanococcus aeolicus <400> 273 Met Lys Ile Leu Ile Ile Cys Lys Ser Val His His Gly Asn Thr Lys 1 5 10 15 Lys Ile Ala Asp Ala Met Ala Glu Val Leu Asn Ala Glu Val Ile Ala 20 25 30 Pro Glu Asn Val Ser Ser Glu Asp Ile Lys Lys Tyr Asp Leu Val Gly 35 40 45 Phe Gly Ser Gly Ile Tyr Ile Gly Lys His His Lys Lys Leu Leu Lys 50 55 60 Leu Ala Asp Asn Leu Pro Asn Gly Glu Asn Lys Thr Val Phe Val Phe 65 70 75 80 Ser Thr Ser Asp Asn Trp Lys Gln Asn Tyr His Lys Pro Leu Met Asp 85 90 95 Lys Leu Asn Ser Arg Gly Tyr Lys Thr Val Gly Glu Phe Asn Cys Lys 100 105 110 Gly Phe Asp Asp Trp Phe Ile Phe Lys Leu Ile Gly Gly Arg Asn Lys 115 120 125 Gly His Pro Asn Lys Lys Asp Ile Glu Asn Ala Lys Lys Phe Ala Glu 130 135 140 Asn Ile Lys Asn Ile Glu Asn Ile 145 150 <210> 274 <211> 336 <212> DNA <213> Escherichia coli <220> <221> CDS <222> (1)..(336) <223> fdx gene from E. coli encoding ferredoxin <400> 274 atg cca aag att gtt att ttg cct cat cag gat ctc tgc cct gat ggc 48 Met Pro Lys Ile Val Ile Leu Pro His Gln Asp Leu Cys Pro Asp Gly 1 5 10 15 gct gtt ctg gaa gct aat agc ggt gaa acc att ctc gac gca gct ctg 96 Ala Val Leu Glu Ala Asn Ser Gly Glu Thr Ile Leu Asp Ala Ala Leu 20 25 30 cgt aac ggt atc gag att gaa cac gcc tgt gaa aaa tcc tgt gct tgc 144 Arg Asn Gly Ile Glu Ile Glu His Ala Cys Glu Lys Ser Cys Ala Cys 35 40 45 acc acc tgc cac tgc atc gtt cgt gaa ggt ttt gac tca ctg ccg gaa 192 Thr Thr Cys His Cys Ile Val Arg Glu Gly Phe Asp Ser Leu Pro Glu 50 55 60 agc tca gag cag gaa gac gac atg ctg gac aaa gcc tgg gga ctg gag 240 Ser Ser Glu Gln Glu Asp Asp Met Leu Asp Lys Ala Trp Gly Leu Glu 65 70 75 80 ccg gaa agc cgt tta agc tgc cag gcg cgc gtt acc gac gaa gat tta 288 Pro Glu Ser Arg Leu Ser Cys Gln Ala Arg Val Thr Asp Glu Asp Leu 85 90 95 gta gtc gaa atc ccg cgt tac act atc aac cat gcg cgt gag cat taa 336 Val Val Glu Ile Pro Arg Tyr Thr Ile Asn His Ala Arg Glu His 100 105 110 <210> 275 <211> 111 <212> PRT <213> Escherichia coli <400> 275 Met Pro Lys Ile Val Ile Leu Pro His Gln Asp Leu Cys Pro Asp Gly 1 5 10 15 Ala Val Leu Glu Ala Asn Ser Gly Glu Thr Ile Leu Asp Ala Ala Leu 20 25 30 Arg Asn Gly Ile Glu Ile Glu His Ala Cys Glu Lys Ser Cys Ala Cys 35 40 45 Thr Thr Cys His Cys Ile Val Arg Glu Gly Phe Asp Ser Leu Pro Glu 50 55 60 Ser Ser Glu Gln Glu Asp Asp Met Leu Asp Lys Ala Trp Gly Leu Glu 65 70 75 80 Pro Glu Ser Arg Leu Ser Cys Gln Ala Arg Val Thr Asp Glu Asp Leu 85 90 95 Val Val Glu Ile Pro Arg Tyr Thr Ile Asn His Ala Arg Glu His 100 105 110 <210> 276 <211> 249 <212> DNA <213> Bacillus subtilis <220> <221> CDS <222> (1)..(249) <223> fer gene from B. subtilis encoding ferredoxin <400> 276 atg gca aag tac aca atc gta gac aaa gat aca tgt att gca tgc ggc 48 Met Ala Lys Tyr Thr Ile Val Asp Lys Asp Thr Cys Ile Ala Cys Gly 1 5 10 15 gct tgc gga gct gct gca cca gac att tac gat tac gat gat gaa ggc 96 Ala Cys Gly Ala Ala Ala Pro Asp Ile Tyr Asp Tyr Asp Asp Glu Gly 20 25 30 atc gcg ttc gta acg ctt gat gaa aac aaa ggt gtt gtc gaa gtt cct 144 Ile Ala Phe Val Thr Leu Asp Glu Asn Lys Gly Val Val Glu Val Pro 35 40 45 gag gta ctg gaa gaa gat atg att gac gca ttt gaa gga tgc cct act 192 Glu Val Leu Glu Glu Asp Met Ile Asp Ala Phe Glu Gly Cys Pro Thr 50 55 60 gat tcc atc aaa gtg gcg gat gag cca ttt gaa ggc gac ccg ctt aaa 240 Asp Ser Ile Lys Val Ala Asp Glu Pro Phe Glu Gly Asp Pro Leu Lys 65 70 75 80 ttt gaa tag 249 Phe Glu <210> 277 <211> 82 <212> PRT <213> Bacillus subtilis <400> 277 Met Ala Lys Tyr Thr Ile Val Asp Lys Asp Thr Cys Ile Ala Cys Gly 1 5 10 15 Ala Cys Gly Ala Ala Ala Pro Asp Ile Tyr Asp Tyr Asp Asp Glu Gly 20 25 30 Ile Ala Phe Val Thr Leu Asp Glu Asn Lys Gly Val Val Glu Val Pro 35 40 45 Glu Val Leu Glu Glu Asp Met Ile Asp Ala Phe Glu Gly Cys Pro Thr 50 55 60 Asp Ser Ile Lys Val Ala Asp Glu Pro Phe Glu Gly Asp Pro Leu Lys 65 70 75 80 Phe Glu <210> 278 <211> 321 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(321) <223> fdxB gene from Corynebacterium glutamicum encoding ferredoxin <400> 278 atg tct act att cat ttc att gat cat gct ggc aaa acc cgc acc atc 48 Met Ser Thr Ile His Phe Ile Asp His Ala Gly Lys Thr Arg Thr Ile 1 5 10 15 gag gcg act gtt ggt gat tca gta atg gag acc gca gtc cga aac gga 96 Glu Ala Thr Val Gly Asp Ser Val Met Glu Thr Ala Val Arg Asn Gly 20 25 30 gtg cct gga att gtt gct gaa tgc ggc ggt tcc tta tcg tgt gca acc 144 Val Pro Gly Ile Val Ala Glu Cys Gly Gly Ser Leu Ser Cys Ala Thr 35 40 45 tgc cat gtg ttt gtt gac cct gca cag tat gat gcg ctt ccc cca atg 192 Cys His Val Phe Val Asp Pro Ala Gln Tyr Asp Ala Leu Pro Pro Met 50 55 60 gag gag atg gaa gat gaa atg ctg tgg ggt gct gcc gtg gac cgt gag 240 Glu Glu Met Glu Asp Glu Met Leu Trp Gly Ala Ala Val Asp Arg Glu 65 70 75 80 gat tgc tcc cgt ttg tct tgc caa atc aag gtc acc gaa ggc atg gat 288 Asp Cys Ser Arg Leu Ser Cys Gln Ile Lys Val Thr Glu Gly Met Asp 85 90 95 ctt tcg ttg acc acg cca gaa acg caa gtg tga 321 Leu Ser Leu Thr Thr Pro Glu Thr Gln Val 100 105 <210> 279 <211> 106 <212> PRT <213> Corynebacterium glutamicum <400> 279 Met Ser Thr Ile His Phe Ile Asp His Ala Gly Lys Thr Arg Thr Ile 1 5 10 15 Glu Ala Thr Val Gly Asp Ser Val Met Glu Thr Ala Val Arg Asn Gly 20 25 30 Val Pro Gly Ile Val Ala Glu Cys Gly Gly Ser Leu Ser Cys Ala Thr 35 40 45 Cys His Val Phe Val Asp Pro Ala Gln Tyr Asp Ala Leu Pro Pro Met 50 55 60 Glu Glu Met Glu Asp Glu Met Leu Trp Gly Ala Ala Val Asp Arg Glu 65 70 75 80 Asp Cys Ser Arg Leu Ser Cys Gln Ile Lys Val Thr Glu Gly Met Asp 85 90 95 Leu Ser Leu Thr Thr Pro Glu Thr Gln Val 100 105 <210> 280 <211> 561 <212> DNA <213> Synechocystis PCC6803 <220> <221> CDS <222> (1)..(561) <223> fdx gene from Synechocystis encoding ferredoxin <400> 280 atg acc atg cca cca tta tgg aat tgc tct gtc gcc aac agg gtt aat 48 Met Thr Met Pro Pro Leu Trp Asn Cys Ser Val Ala Asn Arg Val Asn 1 5 10 15 gcc att gtt gcc agt act aag gag gat tgt gtg gct aaa act att aag 96 Ala Ile Val Ala Ser Thr Lys Glu Asp Cys Val Ala Lys Thr Ile Lys 20 25 30 ctc gac ccc att gat tta aaa gtc gcc atc gag acc aac gat aac ctg 144 Leu Asp Pro Ile Asp Leu Lys Val Ala Ile Glu Thr Asn Asp Asn Leu 35 40 45 ctc tcg ggg ttg ctc ggt cag gat tta cgg atc atg aag gag tgt ggt 192 Leu Ser Gly Leu Leu Gly Gln Asp Leu Arg Ile Met Lys Glu Cys Gly 50 55 60 ggt cgg ggt atg tgt gcc act tgt cac gtt tac atc acc gct ggg atg 240 Gly Arg Gly Met Cys Ala Thr Cys His Val Tyr Ile Thr Ala Gly Met 65 70 75 80 gag agt ctt tct ccc ctc aac cgt cgg gag cag cgc acc cta gag gtg 288 Glu Ser Leu Ser Pro Leu Asn Arg Arg Glu Gln Arg Thr Leu Glu Val 85 90 95 atc acc acc cac aat cgt tat tcc cgt ttg gct tgc caa gcc cgg gtg 336 Ile Thr Thr His Asn Arg Tyr Ser Arg Leu Ala Cys Gln Ala Arg Val 100 105 110 ttg gat gaa ggc gtg gtg gtg gaa ttg ccc gct ggg atg tac gtc agt 384 Leu Asp Glu Gly Val Val Val Glu Leu Pro Ala Gly Met Tyr Val Ser 115 120 125 gaa att gag gac atc gag gag ctg att ggc cgt cga gcg gag gaa aat 432 Glu Ile Glu Asp Ile Glu Glu Leu Ile Gly Arg Arg Ala Glu Glu Asn 130 135 140 att ctc aat cct cgg gat ggg agc atc cta gtg gaa aaa ggt aag tta 480 Ile Leu Asn Pro Arg Asp Gly Ser Ile Leu Val Glu Lys Gly Lys Leu 145 150 155 160 att acc cgt tcc atg att agt caa cta gat gac cag tta cag gcg gcc 528 Ile Thr Arg Ser Met Ile Ser Gln Leu Asp Asp Gln Leu Gln Ala Ala 165 170 175 aaa att cag att gtc aac gat acc gat gaa taa 561 Lys Ile Gln Ile Val Asn Asp Thr Asp Glu 180 185 <210> 281 <211> 186 <212> PRT <213> Synechocystis PCC6803 <400> 281 Met Thr Met Pro Pro Leu Trp Asn Cys Ser Val Ala Asn Arg Val Asn 1 5 10 15 Ala Ile Val Ala Ser Thr Lys Glu Asp Cys Val Ala Lys Thr Ile Lys 20 25 30 Leu Asp Pro Ile Asp Leu Lys Val Ala Ile Glu Thr Asn Asp Asn Leu 35 40 45 Leu Ser Gly Leu Leu Gly Gln Asp Leu Arg Ile Met Lys Glu Cys Gly 50 55 60 Gly Arg Gly Met Cys Ala Thr Cys His Val Tyr Ile Thr Ala Gly Met 65 70 75 80 Glu Ser Leu Ser Pro Leu Asn Arg Arg Glu Gln Arg Thr Leu Glu Val 85 90 95 Ile Thr Thr His Asn Arg Tyr Ser Arg Leu Ala Cys Gln Ala Arg Val 100 105 110 Leu Asp Glu Gly Val Val Val Glu Leu Pro Ala Gly Met Tyr Val Ser 115 120 125 Glu Ile Glu Asp Ile Glu Glu Leu Ile Gly Arg Arg Ala Glu Glu Asn 130 135 140 Ile Leu Asn Pro Arg Asp Gly Ser Ile Leu Val Glu Lys Gly Lys Leu 145 150 155 160 Ile Thr Arg Ser Met Ile Ser Gln Leu Asp Asp Gln Leu Gln Ala Ala 165 170 175 Lys Ile Gln Ile Val Asn Asp Thr Asp Glu 180 185 <210> 282 <211> 294 <212> DNA <213> Streptomyces venezuelae <220> <221> CDS <222> (1)..(294) <223> SVEN_7039 gene from Streptomyces venezuelae encoding ferredoxin <400> 282 atg gcg tac gtc gtc acc gac gag tgc atc ggc tgc aag tac acg gac 48 Met Ala Tyr Val Val Thr Asp Glu Cys Ile Gly Cys Lys Tyr Thr Asp 1 5 10 15 tgt gtg gac gtc tgc ccc gtg agc tgt ttc cac gag ggc ccc gag atg 96 Cys Val Asp Val Cys Pro Val Ser Cys Phe His Glu Gly Pro Glu Met 20 25 30 ctc tac atc aac ccc gag gaa tgc atc gac tgc aac gcg tgc gtc gcc 144 Leu Tyr Ile Asn Pro Glu Glu Cys Ile Asp Cys Asn Ala Cys Val Ala 35 40 45 gag tgc ccg ccc gag gcc atc tgg gcg gac gtc gac ctg ccg gag gac 192 Glu Cys Pro Pro Glu Ala Ile Trp Ala Asp Val Asp Leu Pro Glu Asp 50 55 60 aag ctc cag tgg atc gag atc aac gga gag atg agt gcc aag tac ccg 240 Lys Leu Gln Trp Ile Glu Ile Asn Gly Glu Met Ser Ala Lys Tyr Pro 65 70 75 80 gtt ctc cac gag agc cgg ggc ccc cac gga cag ccc tcc agc cag cct 288 Val Leu His Glu Ser Arg Gly Pro His Gly Gln Pro Ser Ser Gln Pro 85 90 95 tcc tga 294 Ser <210> 283 <211> 97 <212> PRT <213> Streptomyces venezuelae <400> 283 Met Ala Tyr Val Val Thr Asp Glu Cys Ile Gly Cys Lys Tyr Thr Asp 1 5 10 15 Cys Val Asp Val Cys Pro Val Ser Cys Phe His Glu Gly Pro Glu Met 20 25 30 Leu Tyr Ile Asn Pro Glu Glu Cys Ile Asp Cys Asn Ala Cys Val Ala 35 40 45 Glu Cys Pro Pro Glu Ala Ile Trp Ala Asp Val Asp Leu Pro Glu Asp 50 55 60 Lys Leu Gln Trp Ile Glu Ile Asn Gly Glu Met Ser Ala Lys Tyr Pro 65 70 75 80 Val Leu His Glu Ser Arg Gly Pro His Gly Gln Pro Ser Ser Gln Pro 85 90 95 Ser <210> 284 <211> 1746 <212> DNA <213> Methanococcus aeolicus <220> <221> CDS <222> (1)..(1746) <223> fdx gene from Methanococcus aeolicus encoding ferredoxin <400> 284 atg ggg ggt gtt atg atg tat aat att aca tac ata aaa gag gat gga 48 Met Gly Gly Val Met Met Tyr Asn Ile Thr Tyr Ile Lys Glu Asp Gly 1 5 10 15 act aaa aaa tca att aaa gtt aaa gaa gga acc aca ata ctt gaa gga 96 Thr Lys Lys Ser Ile Lys Val Lys Glu Gly Thr Thr Ile Leu Glu Gly 20 25 30 gcg ata aaa gcg gga gtt tat att gat gct cca tgt gga acg ggg aaa 144 Ala Ile Lys Ala Gly Val Tyr Ile Asp Ala Pro Cys Gly Thr Gly Lys 35 40 45 tgt ggt aag tgt aaa gtt tta gtg gag aaa ggt tta gaa aat att gat 192 Cys Gly Lys Cys Lys Val Leu Val Glu Lys Gly Leu Glu Asn Ile Asp 50 55 60 aag gat agt att gtg gaa gat gag tat gca ctg gca tgt gtg gca aaa 240 Lys Asp Ser Ile Val Glu Asp Glu Tyr Ala Leu Ala Cys Val Ala Lys 65 70 75 80 gtt tat ggg gac ata tca att aat gtt cca aat ttc caa ggt gtg gtt 288 Val Tyr Gly Asp Ile Ser Ile Asn Val Pro Asn Phe Gln Gly Val Val 85 90 95 tgt aag gat atc acc aac gaa gtt ggt gag cta caa act cga aga gtt 336 Cys Lys Asp Ile Thr Asn Glu Val Gly Glu Leu Gln Thr Arg Arg Val 100 105 110 tgt tca att acc gaa caa tgt aaa ggt gag cta caa aat ctc gag gga 384 Cys Ser Ile Thr Glu Gln Cys Lys Gly Glu Leu Gln Asn Leu Glu Gly 115 120 125 ttt cat ccg atg tct tta aac ccc gat att gga ata aat aaa att act 432 Phe His Pro Met Ser Leu Asn Pro Asp Ile Gly Ile Asn Lys Ile Thr 130 135 140 aca aca gta ttg gaa tca tct aac tat aac cta aca tta gat gca ata 480 Thr Thr Val Leu Glu Ser Ser Asn Tyr Asn Leu Thr Leu Asp Ala Ile 145 150 155 160 aat aag ctt aat tct atg aag tta tcg gac gaa gta act tta ata tta 528 Asn Lys Leu Asn Ser Met Lys Leu Ser Asp Glu Val Thr Leu Ile Leu 165 170 175 aag gga gat aat gtc gtt aat gta gaa aaa gat ttt tct gga att tat 576 Lys Gly Asp Asn Val Val Asn Val Glu Lys Asp Phe Ser Gly Ile Tyr 180 185 190 ggg ctt tca att gat att ggg act aca tct gtt gtt gta tat ctt gtt 624 Gly Leu Ser Ile Asp Ile Gly Thr Thr Ser Val Val Val Tyr Leu Val 195 200 205 gat att tct aaa ggt att gtt tta gat aat att tct ttt tta aat cct 672 Asp Ile Ser Lys Gly Ile Val Leu Asp Asn Ile Ser Phe Leu Asn Pro 210 215 220 cag agg cag ttt ggg gca gat gtt gtt tca aga ata gca tac aac aac 720 Gln Arg Gln Phe Gly Ala Asp Val Val Ser Arg Ile Ala Tyr Asn Asn 225 230 235 240 gga att tta ctg caa aaa aca ctt ata act gaa tta aac gat tct ata 768 Gly Ile Leu Leu Gln Lys Thr Leu Ile Thr Glu Leu Asn Asp Ser Ile 245 250 255 tca aaa tta tgt tca aac aat aac ata aaa atg gat aat att tat gaa 816 Ser Lys Leu Cys Ser Asn Asn Asn Ile Lys Met Asp Asn Ile Tyr Glu 260 265 270 gtt agt gtg gta gga aac act gct atg ata cac ttc ttt tat gga ata 864 Val Ser Val Val Gly Asn Thr Ala Met Ile His Phe Phe Tyr Gly Ile 275 280 285 gtc cca aaa aat ctt gca acc cat cct tat gtt cca aca ttt aaa aac 912 Val Pro Lys Asn Leu Ala Thr His Pro Tyr Val Pro Thr Phe Lys Asn 290 295 300 tca cca tat ctt cct gca aaa gag ttg ggg cta aac cta aga aac gca 960 Ser Pro Tyr Leu Pro Ala Lys Glu Leu Gly Leu Asn Leu Arg Asn Ala 305 310 315 320 tac att tac aca ctt ccg ata ata gga ggt tat gtt ggg gca gac aca 1008 Tyr Ile Tyr Thr Leu Pro Ile Ile Gly Gly Tyr Val Gly Ala Asp Thr 325 330 335 gtt gga gca att tta tca tct gaa atg cat aaa aaa gat gat ata agt 1056 Val Gly Ala Ile Leu Ser Ser Glu Met His Lys Lys Asp Asp Ile Ser 340 345 350 ctc ctt ata gat att ggc aca aat ggg gaa att gtt tta ggg aat aaa 1104 Leu Leu Ile Asp Ile Gly Thr Asn Gly Glu Ile Val Leu Gly Asn Lys 355 360 365 gaa aag tta tta acc tgt tca tgt gca gca ggt cct gca ttt gag ggt 1152 Glu Lys Leu Leu Thr Cys Ser Cys Ala Ala Gly Pro Ala Phe Glu Gly 370 375 380 gtc agc ata gag cat ggg aca aat gct aga gag ggg gca gta tgt aga 1200 Val Ser Ile Glu His Gly Thr Asn Ala Arg Glu Gly Ala Val Cys Arg 385 390 395 400 gta aaa ata gat gaa aat aac ata tac tat gag acc ata gga aat aaa 1248 Val Lys Ile Asp Glu Asn Asn Ile Tyr Tyr Glu Thr Ile Gly Asn Lys 405 410 415 acg ccc cct att gga ata tgc ggg tct gga ata ata gat att gta gct 1296 Thr Pro Pro Ile Gly Ile Cys Gly Ser Gly Ile Ile Asp Ile Val Ala 420 425 430 gaa ttt tta aaa tcc gga tta att aat aaa acc ggt aga ttt act gga 1344 Glu Phe Leu Lys Ser Gly Leu Ile Asn Lys Thr Gly Arg Phe Thr Gly 435 440 445 gaa cat aaa aac tta aag gaa aat aaa ttt atc att gaa gat tct att 1392 Glu His Lys Asn Leu Lys Glu Asn Lys Phe Ile Ile Glu Asp Ser Ile 450 455 460 tat ttc aca cag ggc gat att agg gaa gta cag ctt gca aaa ggg gca 1440 Tyr Phe Thr Gln Gly Asp Ile Arg Glu Val Gln Leu Ala Lys Gly Ala 465 470 475 480 ata tat gca gga ata aaa att ctc tgt tat gaa tat gga ata agt atg 1488 Ile Tyr Ala Gly Ile Lys Ile Leu Cys Tyr Glu Tyr Gly Ile Ser Met 485 490 495 gaa gat ata tct aat gta tat gtt act gga gca ttt gga tgt cat atc 1536 Glu Asp Ile Ser Asn Val Tyr Val Thr Gly Ala Phe Gly Cys His Ile 500 505 510 gat gtt gaa aat gca aag att atc gga ctt tta ccg gat ttg gat aat 1584 Asp Val Glu Asn Ala Lys Ile Ile Gly Leu Leu Pro Asp Leu Asp Asn 515 520 525 ata ttg agt att gat aat gct gct gga agg ggg act ata atg gct tta 1632 Ile Leu Ser Ile Asp Asn Ala Ala Gly Arg Gly Thr Ile Met Ala Leu 530 535 540 cta tct aaa aaa att aga aat gaa gcc gat aag ttg gca aaa aat acg 1680 Leu Ser Lys Lys Ile Arg Asn Glu Ala Asp Lys Leu Ala Lys Asn Thr 545 550 555 560 aaa tat att gaa tta agt agt cat gat aat ttt gaa agt gag ttc ata 1728 Lys Tyr Ile Glu Leu Ser Ser His Asp Asn Phe Glu Ser Glu Phe Ile 565 570 575 tct gcc ctt ggg ttt taa 1746 Ser Ala Leu Gly Phe 580 <210> 285 <211> 581 <212> PRT <213> Methanococcus aeolicus <400> 285 Met Gly Gly Val Met Met Tyr Asn Ile Thr Tyr Ile Lys Glu Asp Gly 1 5 10 15 Thr Lys Lys Ser Ile Lys Val Lys Glu Gly Thr Thr Ile Leu Glu Gly 20 25 30 Ala Ile Lys Ala Gly Val Tyr Ile Asp Ala Pro Cys Gly Thr Gly Lys 35 40 45 Cys Gly Lys Cys Lys Val Leu Val Glu Lys Gly Leu Glu Asn Ile Asp 50 55 60 Lys Asp Ser Ile Val Glu Asp Glu Tyr Ala Leu Ala Cys Val Ala Lys 65 70 75 80 Val Tyr Gly Asp Ile Ser Ile Asn Val Pro Asn Phe Gln Gly Val Val 85 90 95 Cys Lys Asp Ile Thr Asn Glu Val Gly Glu Leu Gln Thr Arg Arg Val 100 105 110 Cys Ser Ile Thr Glu Gln Cys Lys Gly Glu Leu Gln Asn Leu Glu Gly 115 120 125 Phe His Pro Met Ser Leu Asn Pro Asp Ile Gly Ile Asn Lys Ile Thr 130 135 140 Thr Thr Val Leu Glu Ser Ser Asn Tyr Asn Leu Thr Leu Asp Ala Ile 145 150 155 160 Asn Lys Leu Asn Ser Met Lys Leu Ser Asp Glu Val Thr Leu Ile Leu 165 170 175 Lys Gly Asp Asn Val Val Asn Val Glu Lys Asp Phe Ser Gly Ile Tyr 180 185 190 Gly Leu Ser Ile Asp Ile Gly Thr Thr Ser Val Val Val Tyr Leu Val 195 200 205 Asp Ile Ser Lys Gly Ile Val Leu Asp Asn Ile Ser Phe Leu Asn Pro 210 215 220 Gln Arg Gln Phe Gly Ala Asp Val Val Ser Arg Ile Ala Tyr Asn Asn 225 230 235 240 Gly Ile Leu Leu Gln Lys Thr Leu Ile Thr Glu Leu Asn Asp Ser Ile 245 250 255 Ser Lys Leu Cys Ser Asn Asn Asn Ile Lys Met Asp Asn Ile Tyr Glu 260 265 270 Val Ser Val Val Gly Asn Thr Ala Met Ile His Phe Phe Tyr Gly Ile 275 280 285 Val Pro Lys Asn Leu Ala Thr His Pro Tyr Val Pro Thr Phe Lys Asn 290 295 300 Ser Pro Tyr Leu Pro Ala Lys Glu Leu Gly Leu Asn Leu Arg Asn Ala 305 310 315 320 Tyr Ile Tyr Thr Leu Pro Ile Ile Gly Gly Tyr Val Gly Ala Asp Thr 325 330 335 Val Gly Ala Ile Leu Ser Ser Glu Met His Lys Lys Asp Asp Ile Ser 340 345 350 Leu Leu Ile Asp Ile Gly Thr Asn Gly Glu Ile Val Leu Gly Asn Lys 355 360 365 Glu Lys Leu Leu Thr Cys Ser Cys Ala Ala Gly Pro Ala Phe Glu Gly 370 375 380 Val Ser Ile Glu His Gly Thr Asn Ala Arg Glu Gly Ala Val Cys Arg 385 390 395 400 Val Lys Ile Asp Glu Asn Asn Ile Tyr Tyr Glu Thr Ile Gly Asn Lys 405 410 415 Thr Pro Pro Ile Gly Ile Cys Gly Ser Gly Ile Ile Asp Ile Val Ala 420 425 430 Glu Phe Leu Lys Ser Gly Leu Ile Asn Lys Thr Gly Arg Phe Thr Gly 435 440 445 Glu His Lys Asn Leu Lys Glu Asn Lys Phe Ile Ile Glu Asp Ser Ile 450 455 460 Tyr Phe Thr Gln Gly Asp Ile Arg Glu Val Gln Leu Ala Lys Gly Ala 465 470 475 480 Ile Tyr Ala Gly Ile Lys Ile Leu Cys Tyr Glu Tyr Gly Ile Ser Met 485 490 495 Glu Asp Ile Ser Asn Val Tyr Val Thr Gly Ala Phe Gly Cys His Ile 500 505 510 Asp Val Glu Asn Ala Lys Ile Ile Gly Leu Leu Pro Asp Leu Asp Asn 515 520 525 Ile Leu Ser Ile Asp Asn Ala Ala Gly Arg Gly Thr Ile Met Ala Leu 530 535 540 Leu Ser Lys Lys Ile Arg Asn Glu Ala Asp Lys Leu Ala Lys Asn Thr 545 550 555 560 Lys Tyr Ile Glu Leu Ser Ser His Asp Asn Phe Glu Ser Glu Phe Ile 565 570 575 Ser Ala Leu Gly Phe 580 <210> 286 <211> 720 <212> DNA <213> Synthetic <220> <221> CDS <222> (1)..(720) <223> Synthetic gpf gene encoding GFP <400> 286 atg cgt aaa ggc gaa gag ctg ttc act ggt gtc gtc cct att ctg gtg 48 Met Arg Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 1 5 10 15 gaa ctg gat ggt gat gtc aac ggt cat aag ttt tcc gtg cgt ggc gag 96 Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu 20 25 30 ggt gaa ggt gac gca act aat ggt aaa ctg acg ctg aag ttc atc tgt 144 Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile Cys 35 40 45 act act ggt aaa ctg ccg gta cct tgg ccg act ctg gta acg acg ctg 192 Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 50 55 60 act tat ggt gtt cag tgc ttt gct cgt tat ccg gac cat atg aag cag 240 Thr Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 65 70 75 80 cat gac ttc ttc aag tcc gcc atg ccg gaa ggc tat gtg cag gaa cgc 288 His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 85 90 95 acg att tcc ttt aag gat gac ggc acg tac aaa acg cgt gcg gaa gtg 336 Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu Val 100 105 110 aaa ttt gaa ggc gat acc ctg gta aac cgc att gag ctg aaa ggc att 384 Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 115 120 125 gac ttt aaa gaa gac ggc aat atc ctg ggc cat aag ctg gaa tac aat 432 Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 130 135 140 ttt aac agc cac aat gtt tac atc acc gcc gat aaa caa aaa aat ggc 480 Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly 145 150 155 160 att aaa gcg aat ttt aaa att cgc cac aac gtg gag gat ggc agc gtg 528 Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser Val 165 170 175 cag ctg gct gat cac tac cag caa aac act cca atc ggt gat ggt cct 576 Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 180 185 190 gtt ctg ctg cca gac aat cac tat ctg agc acg caa agc gtt ctg tct 624 Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Val Leu Ser 195 200 205 aaa gat ccg aac gag aaa cgc gat cat atg gtt ctg ctg gag ttc gta 672 Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 210 215 220 acc gca gcg ggc atc acg cat ggt atg gat gaa ctg tac aaa tga tga 720 Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 225 230 235 <210> 287 <211> 238 <212> PRT <213> Synthetic <400> 287 Met Arg Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 1 5 10 15 Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu 20 25 30 Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile Cys 35 40 45 Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 50 55 60 Thr Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 65 70 75 80 His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 85 90 95 Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu Val 100 105 110 Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 115 120 125 Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 130 135 140 Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly 145 150 155 160 Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser Val 165 170 175 Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 180 185 190 Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Val Leu Ser 195 200 205 Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 210 215 220 Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 225 230 235 <210> 288 <211> 1486 <212> DNA <213> Escherichia coli <220> <221> promoter <222> (1)..(36) <223> apFAB306 promoter <220> <221> CDS <222> (73)..(603) <223> fldA gene encoding flavodoxin <220> <221> CDS <222> (620)..(1366) <223> flpr gene encoding flavodoxin/ferredoxin reductase <220> <221> terminator <222> (1400)..(1486) <400> 288 ttgacaatta atcatccggc tcgtagtgtt tgtggatggc agtggctagc ggccgcagag 60 gttatttcac tc atg gct atc act ggc atc ttt ttc ggc agc gac acc ggt 111 Met Ala Ile Thr Gly Ile Phe Phe Gly Ser Asp Thr Gly 1 5 10 aat acc gaa aat atc gca aaa atg att caa aaa cag ctt ggt aaa gac 159 Asn Thr Glu Asn Ile Ala Lys Met Ile Gln Lys Gln Leu Gly Lys Asp 15 20 25 gtt gcc gat gtc cat gac att gca aaa agc agc aaa gaa gat ctg gaa 207 Val Ala Asp Val His Asp Ile Ala Lys Ser Ser Lys Glu Asp Leu Glu 30 35 40 45 gct tat gac att ctg ctg ctg ggc atc cca acc tgg tat tac ggc gaa 255 Ala Tyr Asp Ile Leu Leu Leu Gly Ile Pro Thr Trp Tyr Tyr Gly Glu 50 55 60 gcg cag tgt gac tgg gat gac ttc ttc ccg act ctc gaa gag att gat 303 Ala Gln Cys Asp Trp Asp Asp Phe Phe Pro Thr Leu Glu Glu Ile Asp 65 70 75 ttc aac ggc aaa ctg gtt gcg ctg ttt ggt tgt ggt gac cag gaa gat 351 Phe Asn Gly Lys Leu Val Ala Leu Phe Gly Cys Gly Asp Gln Glu Asp 80 85 90 tac gcc gaa tat ttc tgc gac gca ttg ggc acc atc cgc gac atc att 399 Tyr Ala Glu Tyr Phe Cys Asp Ala Leu Gly Thr Ile Arg Asp Ile Ile 95 100 105 gaa ccg cgc ggt gca acc atc gtt ggt cac tgg cca act gcg ggc tat 447 Glu Pro Arg Gly Ala Thr Ile Val Gly His Trp Pro Thr Ala Gly Tyr 110 115 120 125 cat ttc gaa gca tca aaa ggt ctg gca gat gac gac cac ttt gtc ggt 495 His Phe Glu Ala Ser Lys Gly Leu Ala Asp Asp Asp His Phe Val Gly 130 135 140 ctg gct atc gac gaa gac cgt cag ccg gaa ctg acc gct gaa cgt gta 543 Leu Ala Ile Asp Glu Asp Arg Gln Pro Glu Leu Thr Ala Glu Arg Val 145 150 155 gaa aaa tgg gtt aaa cag att tct gaa gag ttg cat ctc gac gaa att 591 Glu Lys Trp Val Lys Gln Ile Ser Glu Glu Leu His Leu Asp Glu Ile 160 165 170 ctc aat gcc tga aaaacaggag aaaaac atg gct gat tgg gta aca ggc aaa 643 Leu Asn Ala Met Ala Asp Trp Val Thr Gly Lys 175 180 gtc act aaa gtg cag aac tgg acc gac gcc ctg ttt agt ctc acc gtt 691 Val Thr Lys Val Gln Asn Trp Thr Asp Ala Leu Phe Ser Leu Thr Val 185 190 195 200 cac gcc ccc gtg ctt ccg ttt acc gcc ggg caa ttt acc aag ctt ggc 739 His Ala Pro Val Leu Pro Phe Thr Ala Gly Gln Phe Thr Lys Leu Gly 205 210 215 ctt gaa atc gac ggc gaa cgc gtc cag cgc gcc tac tcc tat gta aac 787 Leu Glu Ile Asp Gly Glu Arg Val Gln Arg Ala Tyr Ser Tyr Val Asn 220 225 230 tcg ccc gat aat ccc gat ctg gag ttt tac ctg gtc acc gtc ccc gat 835 Ser Pro Asp Asn Pro Asp Leu Glu Phe Tyr Leu Val Thr Val Pro Asp 235 240 245 ggc aaa tta agc cca cga ctg gcg gca ctg aaa cca ggc gat gaa gtg 883 Gly Lys Leu Ser Pro Arg Leu Ala Ala Leu Lys Pro Gly Asp Glu Val 250 255 260 cag gtg gtt agc gaa gcg gca gga ttc ttt gtg ctc gat gaa gtg ccg 931 Gln Val Val Ser Glu Ala Ala Gly Phe Phe Val Leu Asp Glu Val Pro 265 270 275 280 cac tgc gaa acg cta tgg atg ctg gca acc ggt aca gcg att ggc cct 979 His Cys Glu Thr Leu Trp Met Leu Ala Thr Gly Thr Ala Ile Gly Pro 285 290 295 tat tta tcg att ctg caa cta ggt aaa gat tta gat cgc ttc aaa aat 1027 Tyr Leu Ser Ile Leu Gln Leu Gly Lys Asp Leu Asp Arg Phe Lys Asn 300 305 310 ctg gtc ctg gtg cac gcc gca cgt tat gcc gcc gac tta agc tat ttg 1075 Leu Val Leu Val His Ala Ala Arg Tyr Ala Ala Asp Leu Ser Tyr Leu 315 320 325 cca ctg atg cag gaa ctg gaa aaa cgc tac gaa gga aaa ctg cgc att 1123 Pro Leu Met Gln Glu Leu Glu Lys Arg Tyr Glu Gly Lys Leu Arg Ile 330 335 340 cag acg gtg gtc agt cgg gaa acg gca gcg ggg tcg ctc acc gga cgg 1171 Gln Thr Val Val Ser Arg Glu Thr Ala Ala Gly Ser Leu Thr Gly Arg 345 350 355 360 ata ccg gca tta att gaa agt ggg gaa ctg gaa agc acg att ggc ctg 1219 Ile Pro Ala Leu Ile Glu Ser Gly Glu Leu Glu Ser Thr Ile Gly Leu 365 370 375 ccg atg aat aaa gaa acc agc cat gtg atg ctg tgc ggc aat cca cag 1267 Pro Met Asn Lys Glu Thr Ser His Val Met Leu Cys Gly Asn Pro Gln 380 385 390 atg gtg cgc gat aca caa cag ttg ctg aaa gag acc cgg cag atg acg 1315 Met Val Arg Asp Thr Gln Gln Leu Leu Lys Glu Thr Arg Gln Met Thr 395 400 405 aaa cat tta cgt cgc cga ccg ggc cat atg aca gcg gag cat tac tgg 1363 Lys His Leu Arg Arg Arg Pro Gly His Met Thr Ala Glu His Tyr Trp 410 415 420 taa tagcttcata tggtccacag gacactcgtt gctttcacca tgcgtaaagc 1416 aatcagatac ccagcccgcc taatgagcgg gctttttttt gaacaaaatt agagaataac 1476 aatgcaaaca 1486 <210> 289 <211> 176 <212> PRT <213> Escherichia coli <400> 289 Met Ala Ile Thr Gly Ile Phe Phe Gly Ser Asp Thr Gly Asn Thr Glu 1 5 10 15 Asn Ile Ala Lys Met Ile Gln Lys Gln Leu Gly Lys Asp Val Ala Asp 20 25 30 Val His Asp Ile Ala Lys Ser Ser Lys Glu Asp Leu Glu Ala Tyr Asp 35 40 45 Ile Leu Leu Leu Gly Ile Pro Thr Trp Tyr Tyr Gly Glu Ala Gln Cys 50 55 60 Asp Trp Asp Asp Phe Phe Pro Thr Leu Glu Glu Ile Asp Phe Asn Gly 65 70 75 80 Lys Leu Val Ala Leu Phe Gly Cys Gly Asp Gln Glu Asp Tyr Ala Glu 85 90 95 Tyr Phe Cys Asp Ala Leu Gly Thr Ile Arg Asp Ile Ile Glu Pro Arg 100 105 110 Gly Ala Thr Ile Val Gly His Trp Pro Thr Ala Gly Tyr His Phe Glu 115 120 125 Ala Ser Lys Gly Leu Ala Asp Asp Asp His Phe Val Gly Leu Ala Ile 130 135 140 Asp Glu Asp Arg Gln Pro Glu Leu Thr Ala Glu Arg Val Glu Lys Trp 145 150 155 160 Val Lys Gln Ile Ser Glu Glu Leu His Leu Asp Glu Ile Leu Asn Ala 165 170 175 <210> 290 <211> 248 <212> PRT <213> Escherichia coli <400> 290 Met Ala Asp Trp Val Thr Gly Lys Val Thr Lys Val Gln Asn Trp Thr 1 5 10 15 Asp Ala Leu Phe Ser Leu Thr Val His Ala Pro Val Leu Pro Phe Thr 20 25 30 Ala Gly Gln Phe Thr Lys Leu Gly Leu Glu Ile Asp Gly Glu Arg Val 35 40 45 Gln Arg Ala Tyr Ser Tyr Val Asn Ser Pro Asp Asn Pro Asp Leu Glu 50 55 60 Phe Tyr Leu Val Thr Val Pro Asp Gly Lys Leu Ser Pro Arg Leu Ala 65 70 75 80 Ala Leu Lys Pro Gly Asp Glu Val Gln Val Val Ser Glu Ala Ala Gly 85 90 95 Phe Phe Val Leu Asp Glu Val Pro His Cys Glu Thr Leu Trp Met Leu 100 105 110 Ala Thr Gly Thr Ala Ile Gly Pro Tyr Leu Ser Ile Leu Gln Leu Gly 115 120 125 Lys Asp Leu Asp Arg Phe Lys Asn Leu Val Leu Val His Ala Ala Arg 130 135 140 Tyr Ala Ala Asp Leu Ser Tyr Leu Pro Leu Met Gln Glu Leu Glu Lys 145 150 155 160 Arg Tyr Glu Gly Lys Leu Arg Ile Gln Thr Val Val Ser Arg Glu Thr 165 170 175 Ala Ala Gly Ser Leu Thr Gly Arg Ile Pro Ala Leu Ile Glu Ser Gly 180 185 190 Glu Leu Glu Ser Thr Ile Gly Leu Pro Met Asn Lys Glu Thr Ser His 195 200 205 Val Met Leu Cys Gly Asn Pro Gln Met Val Arg Asp Thr Gln Gln Leu 210 215 220 Leu Lys Glu Thr Arg Gln Met Thr Lys His Leu Arg Arg Arg Pro Gly 225 230 235 240 His Met Thr Ala Glu His Tyr Trp 245 <210> 291 <211> 36 <212> DNA <213> Synthetic <220> <221> promoter <222> (1)..(36) <223> apFAB309 promoter <400> 291 ttgacaatta atcatccggc tcgtagtgtc tgtgga 36 <210> 292 <211> 87 <212> DNA <213> Synthetic <220> <221> terminator <222> (1)..(87) <223> apFAB378 terminator <400> 292 ttcaccatgc gtaaagcaat cagataccca gcccgcctaa tgagcgggct tttttttgaa 60 caaaattaga gaataacaat gcaaaca 87

Claims (18)

  1. 하기를 포함하는, 비오틴 또는 리포산 또는 티아민의 증가된 생산을 위한 유전자 변형 박테리아:
    a) 돌연변이 IscR 폴리펩티드를 코딩하는 유전자 변형 내인성 iscR 유전자로서,
    상기 돌연변이 IscR 폴리펩티드의 아미노산 서열은 서열번호 2, 4, 6, 8, 10, 12 및 14로 이루어진 군으로부터 선택된 서열과 적어도 80% 아미노산 서열 상동성을 갖는 것이고, 상기 아미노산 서열은 i) L15X, C92X, C98X, C104X, 및 H107X로 이루어진 군으로부터 선택된 적어도 하나의 아미노산 치환을 갖는 것이고; 상기 X는 서열번호 2, 4, 6, 8, 10, 12 및 14에 상응하는 아미노산 잔기 이외의 임의의 아미노산인 것인, 유전자; 및
    b) 적어도 하나의 전이 유전자로서,
    ii) 비오틴 신타아제 (EC 2.8.1.6) 활성을 갖는 폴리펩티드;
    iii) 리포산 신타아제 (EC2.8.1.8) 활성을 갖는 폴리펩티드;
    iv) HMP-P 신타아제 (EC 4.1.99.17) 활성을 갖는 폴리펩티드; 및
    v) 티로신 리아제 (EC 4.1.99.19) 활성을 갖는 폴리펩티드로 이루어진 군으로부터 선택된 폴리펩티드를 코딩하는 적어도 하나의 전이 유전자.
  2. 청구항 1에 있어서, 상기 돌연변이 IscR 폴리펩티드에서 상기 적어도 하나의 아미노산 치환은 다음으로 이루어진 군으로부터 선택되는 것인, 비오틴 또는 리포산 또는 티아민의 증가된 생산을 위한 유전자 변형 박테리아:
    a. L15X, 상기 X는 F, Y, M 및 W 중 어느 하나임;
    b. C92X, 상기 X는 Y, A, M, F 및 W 중 어느 하나임;
    c. C98X, 상기 X는 A, V, I, L, F 및 W 중 어느 하나임;
    d. C104X, 상기 X는 A V, I, L, F 및 W 중 어느 하나임; 및
    e. H107X; 상기 X는 A, Y, V, I, 및 L 중 어느 하나임.
  3. 청구항 1 또는 2에 있어서, 상기 비오틴 신타아제 활성을 갖는 폴리펩티드 (EC 2.8.1.6)를 코딩하는 적어도 하나의 전이 유전자는 다음으로 이루어진 군으로부터 선택된 하나 이상의 폴리펩티드를 코딩하는 추가적인 전이 유전자를 더 포함하고:
    a. SAM (S-아데노실메티오닌)-의존적 메틸트랜스퍼라제 (EC 2.1.1.197) 활성을 갖는 폴리펩티드;
    b. 7-케토-8-아미노펠라르곤산 (KAPA) 신타아제 (EC 2.3.1.47) 활성을 갖는 폴리펩티드;
    c. 7,8-디아미노펠라르곤산 (DAPA) 신타아제 (EC:2.6.1.62 또는 EC2.6.1.105) 활성을 갖는 폴리펩티드; 및
    d. 데티오비오틴 (DTB) 신타아제 (EC 6.3.3.3) 활성을 갖는 폴리펩티드;
    e. 피멜로일-[아실-운반 단백질] 메틸 에스테르 에스테라제 (EC 3.1.1.85)를 갖는 폴리펩티드; 및
    f. 6-카복시헥사노에이트-CoA 리가제 (EC 6.2.1.14) 활성을 갖는 폴리펩티드
    상기 박테리아는 비오틴의 증가된 생산을 위한 것인 유전자 변형 박테리아.
  4. 청구항 1 또는 2에 있어서, 상기 적어도 하나의 전이 유전자는 리포산 신타아제 (EC 2.8.1.8) 활성을 갖는 폴리펩티드를 코딩하고, 다음으로 이루어진 군으로부터 선택된 하나 이상의 폴리펩티드를 코딩하는 추가적인 전이 유전자를 더 포함하는 것이고:
    a. 옥타노일트랜스퍼라제 (EC 2.3.1.181) 활성을 갖는 폴리펩티드;
    b. 피루브산 탈수소효소 (EC 2.3.1.12)의 디하이드로리포일라이신-잔기 아세틸트랜스퍼라제 성분을 포함하는 폴리펩티드; 및
    c. 리포에이트-단백질 리가아제 A (EC:6.3.1.20) 활성을 갖는 폴리펩티드;
    상기 박테리아는 리포산의 증가된 생산을 위한 것인 유전자 변형 박테리아.
  5. 청구항 1 또는 2에 있어서, 상기 HMP-P 신타아제 (EC 4.1.99.17) 활성을 갖는 폴리펩티드를 코딩하는 적어도 하나의 전이 유전자 및/또는 티로신 리아제 (EC 4.1.99.19) 활성을 갖는 폴리펩티드를 코딩하는 적어도 하나의 전이 유전자는 다음으로 이루어진 군으로부터 선택된 하나 이상의 폴리펩티드를 코딩하는 추가적인 전이 유전자를 더 포함하는 것이고:
    a. ThiS 아데닐트랜스퍼라제 (EC2.7.7.73) 활성을 갖는 ThiF 폴리펩티드;
    b. 티아민 포스페이트 신타아제 (EC 2.5.1.3) 활성을 갖는 ThiE 폴리펩티드;
    c. 티아졸 신타아제 활성 (E.C.2.8.1.10) 활성을 갖는 ThiG 폴리펩티드;
    d. 포스포하이드록시메틸피리미딘 키나아제 (EC 2.7.4.7) 활성을 갖는 ThiD 폴리펩티드;
    e. 글리신 옥시다아제 (EC 1.4.3.19) 활성을 갖는 ThiO 폴리펩티드;
    f. 황-운반 단백질 활성을 갖는 ThiS 폴리펩티드;
    g. 하이드록시에틸티아졸 키나아제 (EC2.7.1.50) 활성을 갖는 ThiM 폴리펩티드; 및
    h. 모노-포스페이트 포스파타아제 (E.C. 3.1.3.-) 활성을 갖는 폴리펩티드;
    상기 박테리아는 티아민의 증가된 생산을 위한 것인 유전자 변형 박테리아.
  6. 청구항 5에 있어서, 상기 박테리아는 다음을 코딩하는 추가적인 전이 유전자를 포함하는 것인 유전자 변형 박테리아:
    a. HMP-P 신타아제 (EC 4.1.99.17) 활성을 갖는 ThiC 폴리펩티드;
    b. 티로신 리아제 (EC 4.1.99.19) 활성을 갖는 ThiH 폴리펩티드 또는 글리신 옥시다아제 (EC 1.4.3.19) 활성을 갖는 ThiO 폴리펩티드;
    c. ThiS 아데닐트랜스퍼라제 (EC 2.7.7.73) 활성을 갖는 ThiF 폴리펩티드;
    d. 티아민 포스페이트 신타아제 (EC 2.5.1.3) 활성을 갖는 ThiE 폴리펩티드;
    e. 티아졸 신타아제 (E.C.2.8.1.10) 활성을 갖는 ThiG 폴리펩티드;
    f. 포스포하이드록시메틸피리미딘 키나아제 (EC 2.7.4.7) 활성을 갖는 ThiD 폴리펩티드;
    g. 황-운반 단백질 활성을 갖는 ThiS 폴리펩티드; 및
    h. 티아민 모노-포스페이트 포스파타아제 (E.C. 3.1.3.-) 활성을 갖는 폴리펩티드.
  7. 청구항 1 내지 6 중 어느 한 항에 있어서, 상기 적어도 하나의 전이 유전자 및 상기 하나 이상의 추가적인 전이 유전자는 항시성 프로모터(constitutive promoter)에 작동 가능하게 연결된 것인 유전자 변형 박테리아.
  8. 청구항 1 내지 7 중 어느 한 항에 있어서, 상기 박테리아는 에셔리키아 (Escherichia), 바실러스 (Bacillus), 브레비박테리움 (Brevibacterium), 버크홀데리아 (Burkholderia), 캄필로박터 (Campylobacter), 코리네박테리움 (Corynebacterium), 슈도모나스 (Pseudomonas), 셀라티아 (Serratia), 락토바실러스 (Lactobacillus), 락토코커스 (Lactocooccus), 아시네토박터 (Acinetobacter), 슈도모나스 (Pseudomonas), 및 아세토박터 (Acetobacter)로 이루어진 군으로부터 선택된 박테리아의 속인 것인 유전자 변형 박테리아.
  9. 하기 단계를 포함하는 비오틴을 생산하는 방법:
    a. 청구항 1 내지 3, 7 및 8 중 어느 한 항에 따른 유전자 변형 박테리아를 배양물을 생산하기 위한 증식 배지 내로 도입하는 단계로서, 여기에서 상기 적어도 하나의 전이 유전자는 비오틴 신타아제 (EC 2.8.1.6) 활성을 갖는 폴리펩티드를 코딩하는 것인 단계;
    b. 상기 배양물을 배양하는 단계; 및
    c. 상기 배양에 의해 생산된 비오틴을 회수하고, 선택적으로 회수된 비오틴을 정제하는 단계.
  10. 리포산을 생산하는 방법으로서,
    a. 청구항 1, 2, 4, 7 및 8 중 어느 한 항에 따른 유전자 변형 박테리아를 배양물을 생산하기 위한 증식 배지 내로 도입하는 단계로서, 여기에서 상기 적어도 하나의 전이 유전자는 리포산 신타아제 (EC 2.8.1.6) 활성을 갖는 폴리펩티드를 코딩하는 것인 단계;
    b. 상기 배양물을 배양하는 단계; 및
    c. 상기 배양에 의해 생산된 리포산을 회수하고, 선택적으로 회수된 리포산을 정제하는 단계를 포함하는 방법.
  11. 티아민을 생산하는 방법으로서,
    a. 청구항 1, 2, 및 5 내지 8 중 어느 한 항에 따른 유전자 변형 박테리아를 배양물을 생산하기 위한 증식 배지 내로 도입하는 단계로서, 여기에서 상기 적어도 하나의 전이 유전자는 HMP-P 신타아제 (EC 4.1.99.17) 활성을 갖는 폴리펩티드 및/또는 티로신 리아제 (EC 4.1.99.19) 활성을 갖는 폴리펩티드를 코딩하는 것인 단계;
    b. 상기 배양물을 배양하는 단계; 및
    c. 상기 배양에 의해 생산된 티아민을 회수하고, 선택적으로 회수된 티아민을 정제하는 단계를 포함하는 방법.
  12. 청구항 9 내지 11 중 어느 한 항에 있어서, 상기 증식 배지는 글루코스, 말토스, 갈락토스, 프럭토스, 수크로스, 아라비노스, 자일로스, 라피노스, 만노스,및 락토스, 또는 이들의 임의의 조합으로부터 선택된 탄소원을 포함하는 것인, 비오틴, 리포산 및 티아민 중 어느 하나를 생산하는 방법.
  13. 유전자 변형 박테리아에서 비오틴, 리포산 또는 티아민 중 어느 하나의 생산을 증가시키기 위한 돌연변이 iscR 폴리펩티드를 코딩하는 유전적으로 변형된 유전자의 용도로서, 상기 박테리아는:
    i. 비오틴 신타아제 (EC 2.8.1.6) 활성을 갖는 폴리펩티드;
    ii. 리포산 신타아제 (EC 2.8.1.8) 활성을 갖는 폴리펩티드;
    iii. HMP-P 신타아제 (EC 4.1.99.17) 활성을 갖는 폴리펩티드; 및
    iv. 티로신 리아제 (EC 4.1.99.19) 활성을 갖는 폴리펩티드로 이루어진 군으로부터 선택된 폴리펩티드를 코딩하는 적어도 하나의 전이유전자를 포함하고 발현하는 것이고;
    상기 유전적으로 변형된 유전자는 돌연변이 IscR 폴리펩티드를 코딩하는 내인성 iscR 유전자이고, 여기에서 상기 돌연변이 IscR 폴리펩티드의 아미노산 서열은 서열번호 2, 4, 6, 8, 10, 12 및 14와 적어도 80% 아미노산 서열 상동성을 갖는 것이고, 상기 아미노산 서열은: L15X, C92X, C98X, C104X, 및 H107X로 이루어진 군으로부터 선택된 적어도 하나의 아미산 치환을 갖는 것이고; 상기 X는 서열번호 2, 4, 6, 8, 10, 12 및 14에 상응하는 아미노산 잔기 이외의 임의의 아미노산인 것인 용도.
  14. 청구항 13에 있어서, 상기 돌연변이 IscR 폴리펩티드에서 상기 적어도 하나의 아미노산 치환은 다음으로 이루어진 군으로부터 선택되는 것인, 유전자 변형 박테리아에서 비오틴, 리포산 또는 티아민 중 어느 하나의 생산을 증가시키기 위한 돌연변이 iscR 폴리펩티드를 코딩하는 유전적으로 변형된 유전자의 용도:
    a. L15X, 상기 X는 F, Y, M 및 W 중 어느 하나임;
    b. C92X, 상기 X는 Y, A, M, F 및 W 중 어느 하나임;
    c. C98X, 상기 X는 A, V, I, L, F 및 W 중 어느 하나임;
    d. C104X, 상기 X는 A V, I, L, F 및 W 중 어느 하나임; 및
    e. H107X; 상기 X는 A, Y, V, I, 및 L 중 어느 하나임.
  15. 비오틴, 리포산 또는 티아민 중 어느 하나의 증가된 생산을 위한, 청구항 1 내지 8 중 어느 한 항에 따른 유전자 변형 박테리아의 용도.
  16. 청구항 1 내지 8 중 어느 한 항에 있어서, 상기 박테리아는 하기 군으로부터 선택된 하나 이상의 유전자를 더 포함하는 것인 유전자 변형 박테리아:
    a. 플라보독신/페레독신-NADP 환원 효소 (EC:1.18.1.2 및 EC 1.19.1.1)를 코딩하는 유전자;
    b. 피루브산-플라보독신/페레독신 산화 환원 효소 (EC 1.2.7)를 코딩하는 유전자;
    c. 플라보독신을 코딩하는 유전자;
    d. 페레독신을 코딩하는 유전자; 및
    e. 플라보독신 및 페레독신-NADP 환원 효소를 코딩하는 유전자;
    여기서, 상기 하나 이상의 유전자는 상기 박테리아에서 상기 하나 이상의 유전자의 발현을 증가시킬 수 있는 비-천연(non-native) 프로모터에 작동 가능하게-연결되는 것이고, 상기 하나 이상의 유전자는 천연 유전자(native gene) 또는 전이 유전자일 수 있다.
  17. 비오틴, 리포산 또는 티아민 중 어느 하나의 증가된 생산을 위한, 청구항 16에 따른 유전자 변형 박테리아의 용도.
  18. 청구항 9 내지 12 중 어느 한 항에 있어서, , 상기 유전자 변형 박테리아는 다음의 군으로부터 선택된 하나 이상의 유전자를 더 포함하는 것인 비오틴, 리포산 및 티아민 중 어느 하나를 생산하는 방법:
    a. 플라보독신/페레독신-NADP 환원 효소 (EC:1.18.1.2 및 EC 1.19.1.1)를 코딩하는 유전자;
    b. 피루브산-플라보독신/페레독신 산화 환원 효소 (EC 1.2.7)를 코딩하는 유전자;
    c. 플라보독신을 코딩하는 유전자;
    d. 페레독신을 코딩하는 유전자; 및
    e. 플라보독신 및 페레독신-NADP 환원 효소;
    상기 하나 이상의 유전자는 상기 박테리아에서 상기 하나 이상의 유전자의 발현을 증가시킬 수 있는 비-천연 프로모터에 작동 가능하게-연결된 것이고, 상기 하나 이상의 유전자는 천연 유전자 또는 전이 유전자일 수 있다.

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