KR20200062476A - Culture incubator system capable of real-time observating and tensile stimulating culture - Google Patents

Abstract

The present invention relates to a culture incubator system capable of tensile stimulation and real-time observation. In particular, while being able to maintain an environment for incubating a culture, the culture incubator system enables real-time observation of the culture via a microscope or a camera while applying mechanical tensile micro-stimuli to the culture. According to the present invention, the culture incubator system comprises: an incubation chamber which is formed such that light can pass in a vertical direction and has an incubation vessel accommodated therein such that the incubation vessel is positioned on a light path where the light passes in the vertical direction; an environment regulation module which regulates and maintains temperature, humidity, and gas atmosphere inside the incubation chamber; a tensile stimulation module which is connected to an edge of one side of the incubation vessel to repeatedly apply vertical stretching and shrinking force to the incubation vessel; and a microscope module which is provided with an object lens or a camera positioned on the light path of the incubation chamber where the light passes in the vertical direction, and which enables observation of the culture being incubated in the incubation vessel.

Description

Culture incubator system capable of tensile stimulation and real-time observation {CULTURE INCUBATOR SYSTEM CAPABLE OF REAL-TIME OBSERVATING AND TENSILE STIMULATING CULTURE}

The present invention relates to a culture incubator system capable of tensile stimulation and real-time observation, and more specifically, while maintaining the environment for culture, it is possible to observe the culture in real time through a microscope and a camera while applying mechanical tensile micro-stimulation to the culture. The present invention relates to a culture incubator system capable of tensile stimulation and real-time observation.

Recently, in the bio industry, cell culture technology has been applied in various fields such as basic research, treatment, and beauty. In addition, in the microbial field, utilization of microbial culture technology is gradually increasing in various fields such as vaccines, therapeutics, hormones, edibles, and new drug development as well as high value-added microbial production.

In general, cell and microbial culture are registered in Korea Patent No. 10-0815074 (2008.03.13, an incubator for cell culture capable of automatic control of the culture environment) and Korea Patent Publication No. 10-2010-0056017 (2010.05.27, cell culture) It is carried out in an incubator that continuously supplies carbon dioxide and maintains constant temperature and humidity as in the incubator).

However, the conventional incubator as described above creates a natural culture and differentiation environment of cells and microorganisms, and there is a problem in that it is impossible to observe changes in cultured cells or tissues in real time only by culturing the cells.

In order to solve the above problem, as in the prior art related to an incubator system capable of observing cells simultaneously with cell culture, as in Korean Patent Publication No. 10-2016-0035625 (2016.04.01, an incubator system capable of observing cultured cells) A technique that can observe the cultured cells in real time has been proposed.

On the other hand, cell signal transduction (mechanotransduction) is caused by the conversion of mechanical energy into electronic and/or biochemical signals. Cells are mechanically sensitive, and physical forces (gravity, tension, compression, and shear) affect the growth and remodeling of all living tissue at the cellular level. Accordingly, Republic of Korea Patent Publication No. 10-2013-0114936 (2013.10.21, cell tension stimulator), Republic of Korea Patent No. 10-1085193 (2011.11.14, cell or tissue tension stimulator) and Korea Patent Registration No. 10-1694619 (2017.01.03, Cell or tissue tension microstimulation device) As for various types of macro-systems, the development of microstimulation devices to study the effect of mechanical stimulation on cell signaling is being developed.

However, in the prior art, the devices for cell cultivation, tensile microstimulation, and real-time observation are separately configured, and it is impossible to observe in real-time while the cells are being tensioned and cultured.

Republic of Korea Patent Publication No. 10-2016-0035625 (2016.04.01): incubator system that can observe the cultured cells Republic of Korea Patent Publication No. 10-2013-0114936 (2013.10.21): cell tension stimulator Republic of Korea Patent No. 10-1085193 (Nov. 14, 2011): Cell or tissue tension stimulator Republic of Korea Patent Registration No. 10-1694619 (2017.01.03): Cell or tissue tension microstimulation device

The present invention was devised by recognizing the above points, and an object of the present invention is to maintain the environment for culturing the culture and to observe the culture in real time through a microscope and a camera while applying mechanical tensile micro stimulation to the culture. It is to provide a culture incubator system capable of tensile stimulation and real-time observation.

In order to achieve the above object, the culture incubator system capable of tensile stimulation and real-time observation according to the present invention is formed so that the light passes in the vertical direction and the culture container is positioned on the optical path where the light passes in the vertical direction. A culture chamber accommodated therein, and an environment control module for controlling and maintaining the temperature, humidity and gas atmosphere inside the culture chamber, and connected to one edge of the culture vessel to repeat the tension and contraction force of a straight line in the culture vessel. Characterized in that it comprises a tensile stimulation module and a microscope module for observing the culture medium cultured in the culture vessel is provided with an objective lens or a camera positioned on the optical path through which light passes in the vertical direction of the culture chamber.

In addition, the culture incubator system capable of tensile stimulation and real-time observation according to the present invention further includes a transfer bed module having a base and a bed transferred in an XY direction orthogonal to a plane on the top of the base, and the culture chamber Is characterized in that it is supported on the transfer bed module and is movable in the XY direction.

In addition, the culture medium incubator system capable of tensile stimulation and real-time observation according to the present invention, wherein the culture chamber is a chamber housing opened up and down and a material through which light is transmitted, so that the chamber housing is covered to cover the upper inlet of the chamber housing. It characterized in that the chamber cover is coupled to the upper inlet is provided.

In addition, the culture medium incubator system capable of real-time observation and tensile stimulation according to the present invention, the tensile stimulation module, a tensioner block coupled to the upper portion of the transfer bed module, and a transfer screw rotatably supported by the tensioner block And, a drive motor connected to the transfer screw to rotate the transfer screw reciprocally, a jack slider moved along the transfer screw by rotation of the transfer screw, and formed of a rigid body, once connected to the jack slider, The other end is characterized in that the connector is connected to the edge of the culture vessel is provided.

In addition, the culture incubator system capable of tensile stimulation and real-time observation according to the present invention, the microscope module, a support block fixed to the base, an arm supported by the support block and moved up and down, and a lower portion of the culture chamber The light source block fixed to the base is provided so as to irradiate light onto an optical path through which light passes in the vertical direction of the culture chamber, and the objective lens or camera is coupled to the tip of the arm and the culture chamber Characterized in that it is located on an optical path through which light passes in the vertical direction.

The culture incubator system capable of real-time observation of tensile stimulation and real-time stimulation according to the present invention can maintain the environment for culturing the culture while observing the culture in real time through a microscope and a camera while applying mechanical tensile micro-stimulation to the culture. It has possible advantages.

1 is a perspective view showing a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
Figure 2 is a perspective view showing a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention
Figure 3 is a plan view showing a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention
Figure 4 is a side view showing a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention
Figure 5 is an enlarged perspective view showing a partial configuration of a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention
Figure 6 is an enlarged perspective view showing a partial configuration of a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention
7 is a perspective view showing the base and the microscope module of the culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention
Figure 8 is a perspective view showing a light source block of a microscope module in a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention
9 is a perspective view showing a transport bed module, a culture chamber and a tension stimulation module of a culture incubator system capable of real-time observation of tensile stimulation and tension according to an embodiment of the present invention
10 is a perspective view showing a state in which a culture chamber and a tensile stimulation module are connected in a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention.
11 is a plan view showing a state in which a culture chamber and a tensile stimulation module are connected in a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention.
12 is a side view showing a state in which a culture chamber and a tensile stimulation module are connected in a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention.
13 is a cross-sectional view showing a state in which a culture chamber and a tensile stimulation module are connected in a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention.
Figure 14 is a perspective view showing a part of the configuration of the tensile stimulation module in a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention
15 is a block diagram showing the configuration of a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention

Hereinafter, a culture incubator system capable of real-time observation and tensile stimulation according to the present invention will be described in more detail with reference to examples shown in the drawings.

1 is a perspective view showing a culture medium incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention, and FIG. 2 shows a culture medium incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention. One perspective view, Figure 3 is a plan view showing a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention, Figure 4 is a tensile stimulation and a culture capable of real-time observation according to an embodiment of the present invention It is a side view showing the incubator system, Figure 5 is a perspective view showing an enlarged partial configuration of a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention, Figure 6 is a perspective view showing an embodiment of the present invention It is a perspective view showing an enlarged part of the configuration of the culture incubator system capable of real-time observation and tensile stimulation, and FIG. 7 shows the base and microscope module of the culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention. 8 is a perspective view showing a light source block of a microscope module in a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention, and FIG. 9 is a tensile stimulation according to an embodiment of the present invention And a transport bed module, a culture chamber, and a tensile stimulation module of a culture incubator system capable of real-time observation, and FIG. 10 shows a culture chamber and a culture chamber in a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention. It is a perspective view showing a state in which the tension stimulation module is connected, and FIG. 11 is a plan view showing a state in which the culture chamber and the tension stimulation module are connected in a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention. 12 is a side view showing a state in which a culture chamber and a tensile stimulation module are connected in a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention, and FIG. 13 is a tensile stimulation according to an embodiment of the present invention, and Culture chamber and tensile stimulation module in a culture incubator system capable of real-time observation This is a cross-sectional view showing a connected state, Figure 14 is a perspective view showing a part of the configuration of a tensile stimulation module in a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention, Figure 15 is a perspective view of the present invention It is a block diagram showing the configuration of a culture incubator system capable of real-time observation and tensile stimulation according to an embodiment.

The culture incubator system capable of tensile stimulation and real-time observation according to the present invention is provided with an environmental control module 5, so that it is possible to maintain the environment for culturing the culture, while the tensile stimulation module 4 applies mechanical tensile micro stimulation to the culture. It is characterized in that it is possible to observe the culture in real time through the microscope module 6 equipped with a microscope and a camera.

Referring to the drawings, the culture incubator system capable of real-time observation and tensile stimulation according to an embodiment of the present invention includes a base 1, a transfer bed module 2, a culture chamber 3, a tension stimulation module 4, and an environment. The control module 5, the microscope module 6 and the control module 7 are provided and configured.

The base 1 is configured to provide a structure in which the transfer bed module 2 and the microscope module 6 are supported and fixed.

Referring to the drawings, the frame 11 is connected to the frame 11 so as to be located at the corner of the cuboid shape, and the guide plate 12 is fixed along the corner at the top of the frame 11. A pair of X-direction linear guides 13 are fixed to the upper portion of the guide plate 12 at positions facing each other. The linear slider 14 is coupled to the X-direction linear guide 13 so as to slide linearly along the X-direction linear guide 13. On the other hand, it is preferable that the linear slider 14 is provided with an encoder scale (not shown in the drawing) so as to measure the moved distance.

On the other hand, at a position below the upper surface of the base 1, a block fixing bar 111 for mounting a light source block 64 for irradiating light toward the bottom surface of the culture vessel 8 to be described below is mounted and coupled. .

The transfer bed module 2 is configured to move the culture chamber 3 mounted on the upper portion of the base 1 and coupled to the upper portion in the X-Y direction. Referring to the drawings, the transfer bed module 2 is composed of an X-direction transfer bed 21 and a Y-direction transfer bed 22.

The X-direction transfer bed 21 is formed of a plate-shaped member and is configured to move in a straight line along the X-direction linear guide 13 of the base 1. The X-direction conveying bed 21 is formed with an opening 211 that penetrates up and down, and a pair of Y-direction straight guides 212 long in the right and left Y-directions along the edge at positions facing each other. ) Is fixed. The X-direction conveying bed 21 is coupled to the X-direction linear guide 13 and is coupled to a linear slider 14 that is slidably moved, and linearly moves in the X-direction along the X-direction linear guide 13. Will be.

The Y-direction transfer bed 22 is formed of a plate-shaped member and is configured to move in a straight line along the Y-direction linear guide 212 of the X-direction transfer bed 21. Like the X-direction conveying bed 21, the Y-direction conveying bed 22 is formed with an opening (not shown in the figure) penetrated up and down. The culture chamber 3 is mounted on the upper portion of the Y-direction transfer bed 22 while covering a position where an opening is formed. The Y-direction conveying bed 22 is coupled to the Y-direction linear guide 212 and coupled to a linear slider (not shown in the drawing) that is slidably moved along the Y-direction linear guide 212. Will move in a straight line.

The culture chamber 3 coupled to the upper portion of the Y-direction transfer bed 22 is an opening 211 formed in the X-direction transfer bed 21 and the Y-direction transfer bed 22 (not shown in the figure) It is located in the X-direction movement of the X-direction transfer bed 21 and the Y-direction movement of the Y-direction transfer bed 22 to move in an orthogonal XY direction.

The culture chamber 3 is configured to form a culture space in which the culture medium is cultured. Referring to the drawings, the culture chamber 3 is configured to be provided with a chamber housing 31 and a cover 32.

The chamber housing 31 is a structure for forming the frame of the culture chamber 3 and has a structure of a substantially rectangular shape and is opened up and down. The culture vessel 8 is positioned inside the culture chamber 3, and light rays for microscopic observation can pass through the inlet opening up and down of the chamber housing 31.

The chamber housing 31 has its inlet opened in the vertical direction and covers the opening (not shown in the figure) of the Y-direction conveying bed 22, while the Y-direction conveying bed is in a position in communication with the opening. It is coupled to the top of (22).

The chamber cover 31 is configured to be coupled to the upper inlet of the chamber housing 31 to cover the upper inlet of the chamber housing 31. As described above, the chamber cover 31 is formed of a material through which light is transmitted, such as glass, so that light beams pass through the inlet opening up and down of the chamber housing 31.

The culture vessel 8 is located inside the chamber cover 31 formed as described above. Since the culture vessel 8 is accommodated in a position close to the lower portion of the chamber cover 31, when the gas for maintaining the environment is injected into the inner space surrounded by the edge of the culture vessel 8 and the chamber cover 31, The gas escapes into the gap between the edge of the culture vessel 8 and the chamber cover 31 while temporarily staying in the inner space surrounded by the edge of the culture vessel 8 and the chamber cover 31, and the chamber housing ( Since it flows to the lower inlet of 31), it becomes possible to control and maintain the culture environment inside the culture vessel 8.

Although not shown in the drawing, the chamber housing 31 has an inlet (not shown in the drawing) for introducing gas supplied from the environmental control module 5 at an appropriate location, and an inlet pipe (not shown in the drawing) at the inlet. City) is connected.

In the culture chamber 3 formed as described above, the light irradiated from the light source block 64 to be described below passes through the light in the vertical direction through the opening opened in the vertical direction of the chamber housing 31. Inside the culture chamber 3, the culture vessel 8 is accommodated to be positioned on an optical path through which light passes in the vertical direction.

The tensile stimulation module (4) is a configuration for repeatedly applying a linear tension and contraction force to the culture vessel (8) located inside the culture chamber (3). Referring to the drawings, the tensile stimulation module 4 is provided with a tensioner block 41, a transfer screw 42, a driving motor 43, a jack slider 44, and a connecting body 45, and the It is coupled to the upper portion of the Y-direction transfer bed 22 at a position close to one side of the culture chamber 3.

The tensioner block 41 is configured to form a structure for the transfer screw 42 to be rotationally supported, the drive motor to be coupled, and the connector 45 to be guided and supported in a straight line. Referring to the drawings, the tensioner block 41 is formed in a shape having a substantially rectangular rim, and the Y-direction transfer bed at a position proximate to one side of the chamber housing 31 of the culture chamber 3 ( 22) is fixedly coupled to the upper portion. A pair of support guides 411 are provided on both sides of the tensioner block 41 in the width direction facing each other for slidingly supporting the connecting body 45 to be linearly moved.

The transfer screw 42 is configured to convert the rotational motion of the drive motor 43 together with the jack slider 44 into a linear motion. Referring to the drawings, each of the transfer screws 42 is rotatably supported by bearings or bushings on each end wall in the front-rear direction of the tensioner block 41.

The drive motor 43 is configured to rotate the transfer screw 42 reciprocally and is fixed to one side of the transfer block 41 so that its rotation shaft is coupled to one end of the transfer screw 42. The transfer screw 42 is reciprocated by the reciprocating rotation of the drive motor 43, and accordingly, the jack slider 44 is linearly moved reciprocally along the transfer screw 42.

The jack slider 44 is configured to convert the rotational motion of the drive motor 43 into a linear motion together with the transfer screw 42. Referring to the drawings, the jack slider 44 is screwed to the transfer screw 42 to be linearly moved along the transfer screw 42 by rotation of the transfer screw 42.

The connecting body 45 is configured to connect the jack slider 44 that is linearly moved and the culture vessel 8 located inside the culture chamber 3 to each other. Referring to the drawings, the connecting body 45 is located on the upper portion of the tensioner block 41 and supported by a support guide 411, the lower portion of which is a slider connecting portion 451 coupled to the jack slider 44 And, one end is connected to the slider connection portion 451 and the other end is composed of a container connection portion 452 connected to the culture vessel 8 located inside the culture chamber 3. The slider connecting portion 451 and the container connecting portion 452 constituting the connecting body 45 are integrally coupled to each other with a material of a rigid body that is less deformed. Therefore, the linear motion of the jack slider 44 exerts a straight force on the connecting body 45 which is a rigid body, and the force is transmitted to the culture vessel 8 to give a fine tensile force and shrinking force to the culture vessel 8. This is transmitted repeatedly.

The container connection portion 452 of the connection body 45 connects the slider connection portion 451 located outside the culture chamber 3 and the culture vessel 8 located inside the culture chamber 3 to each other. , To this end, it should be configured to extend from the outside of the chamber housing 31 constituting the culture chamber 3 to the inside. To this end, the drawing shows an embodiment in which the container connection part 452 is formed to have a bent structure in a zigzag manner so as not to interfere with the chamber housing 31.

The condensation control module 5 is configured to control and maintain the culture environment such as temperature, humidity, gas concentration, etc. inside the culture chamber 3. In the drawing, a state in which a storage tank 51 in which gas and moisture is stored and mixed to control the concentration of gas such as temperature, humidity and carbon dioxide is coupled to the upper portion of the Y-direction transport bed 22 is shown. . In order to control the temperature and humidity, a heating means and a humidifying means are also provided, and the atmosphere gas whose temperature, humidity and gas concentration are adjusted as described above is pumped by the pump 52 to open the inlet pipe connected to the culture chamber 3. It is supplied to the culture chamber (3). On the other hand, the sensor 53 for measuring the temperature, humidity, gas concentration and flow rate, etc. inside the culture chamber 3 for feedback control of the culture environment according to the environmental conditions inside the culture chamber 3 is controlled in the environment. It is provided in the module (53). The sensor 53 is installed at a suitable location, such as the storage tank 51 or the inlet pipe (not shown in the figure) and the interior of the culture chamber (3).

The microscope module 6 is configured to observe in real time the cultures being cultured in the culture vessel 8 located inside the culture chamber 3. Meanwhile, the culture medium refers to an organism or tissue capable of being cultured in the culture vessel 8 such as cells, tissues, and microorganisms.

Referring to the drawings, the microscope module 6 is composed of a support block 61, an arm 62, a microscope 63 or a camera 63', and a light source block 64.

The support block 61 is configured to be fixed to the base 1 to support the arm 62. Referring to the drawings, the support block 61 is fixed to one edge of the frame 11 located on the upper portion of the base 1.

The arm 62 is configured to be supported by the microscope 63 or the camera 63'. Referring to the drawings, the arm 62 is coupled to a support column 611 vertically erected on the support block 61 to be moved up and down. The arm 62 is moved in the vertical direction along the support pillar 611, that is, in the Z-direction, is fixed so as not to move at an appropriate position. The microscope 63 or the camera 63' is attached to the tip of the arm 62.

The microscope 63 has an objective lens 631 in the vertical direction of the culture chamber 3 so that the culture medium cultured in the culture vessel 8 located inside the culture chamber 3 can be visually observed. It is attached to the tip of the arm 62 to be positioned on the optical path through which the light passes.

On the other hand, the camera (63') is a configuration for recording a culture object in real time and storing it as an image or image, it is a configuration that is used at the same time as the microscope (63). Like the microscope 63, the camera 63' is attached to the tip of the arm 62 to be positioned on an optical path through which light passes in the vertical direction of the culture chamber 3. The image photographed by the microscope 63 is transmitted to the control module 7 and stored or edited.

The light source block 64 is configured to irradiate light to the lower portion of the culture vessel 8 located inside the culture chamber 3. Referring to the drawings, the light source block 64 is fixed by being coupled to a block fixing bar 111 provided in the frame 11 to be located under the culture chamber 3.

8 is a perspective view showing the light block 64 in detail, referring to the drawings, the light block 64 is composed of a block body 641, a filter slider 642 and a backlight (not shown in the figure) do.

The block body 641 is configured to form a structure in which the backlight is accommodated and mounted, and the filter slider 642 is slidably mounted. The block body 641 has a structure in which a light irradiation hole 641a is formed upward, and a sliding hole 641b orthogonal to the light irradiation hole 641a is formed on a side portion. The backlight is accommodated and mounted inside the block body 641 to project light through the light irradiation hole 641a. The block body 641 is coupled to and fixed to the block fixing bar 111 provided in the frame 11.

The filter slider 642 is configured such that a plurality of filters 642a are integrally provided so that sliding movement is possible in the sliding hole 641b so that the plurality of filters 642a can be easily changed. Referring to the drawings, the filter slider 642 is formed of a plate-shaped member inserted into the sliding hole 641b, and has a structure in which three filter holes 642b are arranged in a row in the longitudinal direction. A filter 642a for adjusting the wavelength or phase of light is installed in the three filter holes 642b.

The control module 7 controls the operation of the tension stimulation module 4 for applying tensile stimulation to the culture vessel 8 and the environment control module 5 for controlling and maintaining the environment inside the culture chamber 3. It is a configuration to do. For example, the control module 7 controls the operation of the drive motor 43 to adjust the intensity or frequency of tensile stimuli applied to the culture vessel 8. In addition, the control module 7 calculates the physical quantity that needs to be adjusted from the temperature, humidity and gas concentration measured by the sensor 53, and accordingly the humidifying means operates the heating means, and operates the means for mixing the gas. And controlling the operation of the pump for adjusting the flow rate.

In addition, the control module 7 may be configured to manage and edit the operation of the camera 63' of the microscope module 6 and the image or image taken by the camera 63'.

The culture incubator system described above and shown in the drawings and capable of real-time observation of tensile stimulation is only one example for practicing the present invention and should not be interpreted as limiting the technical spirit of the present invention. The protection scope of the present invention is defined only by the matters described in the following claims, and the improved and modified embodiments without departing from the gist of the present invention are apparent to those skilled in the art to which the present invention pertains. It will be said that it belongs to the protection scope of the present invention.

1 base
11 frames
111 block fixing bar
12 Guide plate
13 X-direction straight guide
14 Linear slider
2 Transfer bed module
21 X-direction transfer bed
211 opening
212 Y-direction straight guide
22 Y-direction transfer bed
3 culture chamber
31 chamber housing
32 chamber cover
4 tensile stimulation module
41 tensioner block
411 support guide
42 Transfer screw
43 Drive motor
44 Jack Slider
45 connector
451 slider connection
452 Container connection
5 Environmental control module
51 storage tank
52 pumps
53 sensors
6 Microscope module
61 Support Block
611 support pillar
62 arms
63,63' microscope, camera
631 Objective Lens
64 light source block
7 Control module
8 culture vessel

Claims (5)

A culture chamber formed in such a way that light passes in the vertical direction and the culture container is accommodated therein such that the culture container is positioned on an optical path through which light passes in the vertical direction;
An environmental control module for controlling and maintaining the temperature, humidity and gas atmosphere inside the culture chamber;
A tensile stimulation module connected to one side edge of the culture vessel and repeatedly applying linear tension and contraction force to the culture vessel,
Tensile stimulation and a culture incubator capable of real-time observation, which includes a microscope module for observing the culture medium cultured in the culture vessel, provided with an objective lens or a camera positioned on an optical path where light passes in the vertical direction of the culture chamber. system. According to claim 1,
A transfer bed module further comprising a base and a bed transferred in an XY direction orthogonal to the plane of the upper portion of the base is further included,
The culture chamber is supported by the transfer bed module, it is possible to move in the XY direction Tensile stimulation and culture incubator system capable of real-time observation. The method according to claim 1 or 2,
The culture chamber is a chamber housing opened up and down,
A culture incubator system capable of tensile stimulation and real-time observation, characterized in that a chamber cover is coupled to an upper inlet of the chamber housing to cover the upper inlet of the chamber housing made of a material through which light is transmitted. According to claim 3,
The tensile stimulation module, and a tensioner block coupled to the upper transfer bed module,
A transfer screw rotatably supported by the tensioner block,
A drive motor connected to the transfer screw to rotate the transfer screw reciprocally;
A jack slider moved along the transfer screw by rotation of the transfer screw,
It is formed of a rigid body, one end is connected to the jack slider, and the other end is provided with a connection body connected to the edge of the culture vessel. Tensile stimulation and culture incubator system capable of real-time observation. According to claim 3,
The microscope module includes a support block fixed to the base, an arm supported by the support block and moved up and down, and positioned at a lower portion of the culture chamber to transmit light onto an optical path through which light passes in the vertical direction of the culture chamber. A light source block fixed to the base to be irradiated is provided and configured,
The objective lens or the camera is coupled to the front end of the arm, the culture incubator system capable of real-time observation and tensile stimulation, characterized in that located on an optical path through which light passes in the vertical direction of the culture chamber.

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