KR102156091B1 - Culture incubator system capable of real-time observating and tensile stimulating culture - Google Patents

Abstract

The present invention relates to a culture incubator system capable of tensile stimulation and real-time observation, and more particularly, while maintaining an environment for culture culture, while applying mechanical tensile micro-stimulation to the culture, observe the culture in real time through a microscope and a camera. It relates to a culture incubator system capable of tensile stimulation and real-time observation.
The culture incubator system capable of tensile stimulation and real-time observation according to the present invention includes a culture chamber in which the culture vessel is accommodated so that the culture vessel is positioned on an optical path through which light passes in the vertical direction and the light passes in the vertical direction. , An environment control module for controlling and maintaining the temperature, humidity, and gas atmosphere inside the culture chamber, and a tensile stimulation module connected to one edge of the culture container to repeatedly apply a linear tension and contraction force to the culture container, and the It characterized in that it comprises a microscope module for observing the culture medium cultured in the culture vessel is provided with an objective lens or a camera positioned on an optical path through which light passes in the vertical direction of the culture chamber.

Description

Culture incubator system capable of tensile stimulation and real-time observation {CULTURE INCUBATOR SYSTEM CAPABLE OF REAL-TIME OBSERVATING AND TENSILE STIMULATING CULTURE}

The present invention relates to a culture incubator system capable of tensile stimulation and real-time observation, and more particularly, while maintaining an environment for culture culture, while applying mechanical tensile micro-stimulation to the culture, observe the culture in real time through a microscope and a camera. It relates to a culture incubator system capable of tensile stimulation and real-time observation.

In the recent bio industry, cell culture technology has been applied in various other fields such as basic research, treatment and beauty. In addition, in the microbial field, the utilization of microbial culture technology is gradually increasing in various fields such as vaccine, therapeutic agent, hormone, edible and new drug development, as well as the production of high value-added microorganisms.

In general, cell and microbial culture is conducted in Korean Patent Registration No. 10-0815074 (2008.03.13, incubator for cell culture capable of automatic control of the culture environment) and Korean Patent Application Publication No. 10-2010-0056017 (2010.05.27, cell culture). It is carried out in an incubator that continuously supplies carbon dioxide and maintains a constant temperature and humidity, as in the dragon incubator).

However, the conventional incubator as described above creates an environment for natural culture and differentiation of cells and microorganisms, and has a problem in that it is not possible to observe changes in cells or tissues to be cultured in real time only by culturing cells.

In order to solve the above problem, as in Korean Patent Laid-Open No. 10-2016-0035625 (2016.04.01, incubator system capable of observing cells to be cultured) as a prior art related to an incubator system capable of observing cells simultaneously with cell culture. A technology capable of observing cells to be cultured in real time has been proposed.

On the other hand, cell signal transduction (mechanotransduction) occurs by the conversion of mechanical energy into electrons and / or biochemical signals. Cells are mechanically sensitive, and physical forces (gravity, tension, compression and shear) affect the growth and remodeling of all living tissues at the cellular level. Accordingly, Korean Patent Application Laid-Open No. 10-2013-0114936 (2013.10.21, cell tension stimulator), Korean Patent No. 10-1085193 (2011.11.14, cell or tissue tension stimulator), and Korean Registered Patent No. 10-1694619 As in (2017.01.03, Cell or Tissue Tension Microstimulation Device), microstimulation devices are being developed to study the effects of mechanical stimulation on various types of macro-systems in cell signal transduction.

However, in the prior art, devices for cell culture, tensile microstimulation, and real-time observation are separately configured, and it is impossible to observe in real time while the cells are stretched and cultured.

Republic of Korea Patent Publication No. 10-2016-0035625 (2016.04.01): Incubator system capable of observing cultured cells Republic of Korea Patent Publication No. 10-2013-0114936 (2013.10.21): Cell tension stimulator Korean Patent Registration No. 10-1085193 (2011.11.14): Cell or tissue tension stimulator Republic of Korea Patent Registration No. 10-1694619 (2017.01.03): Cell or tissue tension microstimulation device

The present invention has been conceived by recognizing the above points, and an object of the present invention is to observe the culture in real time through a microscope and a camera while applying a mechanical tensile microstimulation to the culture while maintaining the environment for culturing the culture. It is to provide a culture incubator system capable of tensile stimulation and real-time observation.

In order to achieve the above object, the culture incubator system capable of tensile stimulation and real-time observation according to the present invention is formed to pass light in the vertical direction and the culture container is positioned on an optical path through which light passes in the vertical direction. A culture chamber accommodated therein, an environmental control module that controls and maintains the temperature, humidity, and gas atmosphere inside the culture chamber, and is connected to one edge of the culture container to repetitively provide a linear tension and contraction force to the culture container. It characterized in that it comprises a microscope module for observing the culture medium cultured in the culture vessel is provided with a tensile stimulation module to be applied to, and an objective lens or a camera positioned on an optical path through which light passes in the vertical direction of the culture chamber.

In addition, the culture incubator system capable of tensile stimulation and real-time observation according to the present invention further includes a transfer bed module having a base and a bed transferred in an XY direction orthogonal on a plane of an upper portion of the base, and the culture chamber Is supported by the transfer bed module and is capable of moving in the XY direction.

In addition, in the culture incubator system capable of tensile stimulation and real-time observation according to the present invention, the culture chamber is made of a chamber housing that is open up and down, and a material through which light is transmitted, so as to cover the upper entrance of the chamber housing. It characterized in that it is configured with a chamber cover coupled to the upper inlet.

In addition, in the culture incubator system capable of tensile stimulation and real-time observation according to the present invention, the tensile stimulation module includes a tensioner block coupled to an upper portion of the transfer bed module, and a transfer screw rotatably supported by the tensioner block. And, a drive motor connected to the transfer screw to rotate the transfer screw reciprocally, a jack slider moved along the transfer screw by rotation of the transfer screw, and a rigid body at one end connected to the jack slider, and The other end is characterized in that the structure is provided with a connector connected to the edge of the culture vessel.

In addition, in the culture incubator system capable of tensile stimulation and real-time observation according to the present invention, the microscope module includes a support block fixed to the base, an arm supported by the support block and moved up and down, and a lower portion of the culture chamber. And a light source block fixed to the base so as to irradiate light onto an optical path through which light passes in the vertical direction of the culture chamber, and the objective lens or camera is coupled to the tip of the arm. It is characterized in that it is located on an optical path through which light passes in the vertical direction.

The culture incubator system capable of tensile stimulation and real-time observation according to the present invention by the configuration as described above enables maintenance of the environment for culture culture, while applying mechanical tensile micro-stimulation to the culture material, and observes the culture material in real time through a microscope and a camera. This has possible advantages.

1 is a perspective view showing a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
Figure 2 is a perspective view showing a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
3 is a plan view showing a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
Figure 4 is a side view showing a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
5 is an enlarged perspective view showing a partial configuration of a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
6 is an enlarged perspective view showing a partial configuration of a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
7 is a perspective view showing a base and a microscope module of a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
8 is a perspective view showing a light source block of a microscope module in a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
9 is a perspective view showing a transfer bed module, a culture chamber, and a tensile stimulation module of a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
10 is a perspective view showing a state in which a culture chamber and a tensile stimulation module are connected in a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
11 is a plan view showing a state in which a culture chamber and a tensile stimulation module are connected in a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
12 is a side view showing a state in which a culture chamber and a tensile stimulation module are connected in a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
13 is a cross-sectional view showing a state in which a culture chamber and a tensile stimulation module are connected in a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
14 is a perspective view showing a partial configuration of a tensile stimulation module in a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention
15 is a block diagram showing the configuration of a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention

Hereinafter, a culture incubator system capable of tensile stimulation and real-time observation according to the present invention will be described in more detail with reference to the embodiments shown in the drawings.

1 is a perspective view illustrating a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention, and FIG. 2 is a diagram illustrating a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention. 3 is a plan view showing a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention, and FIG. 4 is a culture material capable of tensile stimulation and real-time observation according to an embodiment of the present invention It is a side view showing the incubator system, Figure 5 is a perspective view showing an enlarged portion of the configuration of a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention, Figure 6 is an embodiment of the present invention It is a perspective view showing an enlarged view of some configurations of a culture incubator system capable of tensile stimulation and real-time observation according to the present invention, and FIG. 7 shows a base and a microscope module of a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention. One perspective view, Figure 8 is a perspective view showing the light source block of the microscope module in the culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention, Figure 9 is a tensile stimulation according to an embodiment of the present invention And a perspective view showing a transfer bed module, a culture chamber, and a tensile stimulation module of a culture incubator system capable of real-time observation, and FIG. 10 is a culture chamber and a culture chamber in a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention. A perspective view showing a state in which the tensile stimulation module is connected, and FIG. 11 is a plan view illustrating a state in which the culture chamber and the tensile stimulation module are connected in a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention. 12 is a side view showing a state in which a culture chamber and a tensile stimulation module are connected in a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention, and FIG. 13 is a tensile stimulation and a tensile stimulation according to an embodiment of the present invention. Culture chamber and tensile stimulation module in a culture incubator system capable of real-time observation This is a cross-sectional view showing a connected state, and FIG. 14 is a perspective view showing a partial configuration of a tensile stimulation module in a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention, and FIG. 15 is an embodiment of the present invention. It is a block diagram showing the configuration of a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment.

The culture incubator system capable of tensile stimulation and real-time observation according to the present invention is provided with an environment control module 5 to maintain the environment for culture culture, while applying mechanical tensile microstimulation to the culture medium with the tensile stimulation module 4 It is characterized in that it is possible to observe the culture in real time through a microscope module 6 equipped with a microscope and a camera.

Referring to the drawings, a culture incubator system capable of tensile stimulation and real-time observation according to an embodiment of the present invention includes a base (1), a transfer bed module (2), a culture chamber (3), a tensile stimulation module (4), and an environment. The control module 5, the microscope module 6 and the control module 7 are provided and configured.

The base 1 is a configuration for providing a structure in which the transfer bed module 2 and the microscope module 6 are supported and fixed.

Referring to the drawings, the frame 11 is connected so that the frame 11 is positioned at the corner of a rectangular parallelepiped shape, and the guide plate 12 is fixed along the corner on the upper portion of the frame 11. A pair of X-direction linear guides 13 are fixed at positions facing each other on the upper part of the guide plate 12. A linear slider 14 is coupled to the X-direction linear guide 13 so as to slide in a straight line along the X-direction linear guide 13. On the other hand, it is preferable that the linear slider 14 is provided with an encoder scale (not shown in the drawing) so as to measure the distance moved.

On the other hand, at a position below the upper surface of the base 1, a block fixing bar 111 is mounted and coupled to a light source block 64 for irradiating light toward the bottom surface of the culture vessel 8 to be described below. .

The transfer bed module 2 is a configuration for moving the culture chamber 3 mounted on the upper part of the base 1 and coupled thereto in the X-Y direction. Referring to the drawings, the transfer bed module 2 is configured with an X-direction transfer bed 21 and a Y-direction transfer bed 22.

The X-direction transfer bed 21 is formed of a plate-shaped member and moves in a straight line along the X-direction linear guide 13 of the base 1. The X-direction transfer bed 21 is formed with an opening 211 penetrating vertically, and a pair of Y-direction linear guides 212 extending along the edges in a position facing each other in the upper portion thereof in the left and right Y-direction ) Is fixed. The X-direction transfer bed 21 is coupled to a linear slider 14 that is coupled to the X-direction linear guide 13 and slides, and moves linearly in the X-direction along the X-direction linear guide 13 It will be.

The Y-direction transfer bed 22 is formed of a plate-shaped member and moves in a straight line along the Y-direction linear guide 212 of the X-direction transfer bed 21. Like the X-direction transfer bed 21, the Y-direction transfer bed 22 has an opening (not shown in the drawing) penetrating vertically. The culture chamber 3 is mounted on the upper part of the Y-direction transfer bed 22 while covering the position where the opening is formed. The Y-direction transfer bed 22 is coupled to a linear slider (not shown in the drawing) coupled to the Y-direction linear guide 212 and slidably moved in the Y-direction along the Y-direction linear guide 212 It will be moved linearly.

The culture chamber 3 coupled to the upper portion of the Y-direction transfer bed 22 is an opening 211 formed in the X-direction transfer bed 21 and the Y-direction transfer bed 22 (not shown in the drawing) It is positioned in the X-direction movement of the X-direction transfer bed 21 and the Y-direction movement of the Y-direction transfer bed 22 to be moved in the orthogonal XY direction.

The culture chamber 3 is a configuration for forming a culture space in which a culture medium is cultured. Referring to the drawings, the culture chamber 3 is configured with a chamber housing 31 and a cover 32.

The chamber housing 31 is a structure for forming a frame of the culture chamber 3 and has a structure that is open up and down with a substantially rectangular rim. The culture vessel 8 is located inside the culture chamber 3, and a light beam for microscopic observation can pass through an inlet opened up and down of the chamber housing 31.

The chamber housing 31 is the Y-direction transfer bed at a position in which the inlet opened in the vertical direction covers the open portion (not shown in the drawing) of the Y-direction transfer bed 22 and communicates with the open portion. It is joined to the top of 22.

The chamber cover 31 is coupled to the upper entrance of the chamber housing 31 to cover the upper entrance of the chamber housing 31. As described above, the chamber cover 31 is formed of a material through which light is transmitted, such as glass, which is opened up and down of the chamber housing 31 so that light rays pass through the inlet.

The culture vessel 8 is located inside the chamber cover 31 formed as described above. Since the culture vessel 8 is accommodated in a position close to the lower portion of the chamber cover 31, when gas for maintaining the environment is injected into the inner space surrounded by the edge of the culture vessel 8 and the chamber cover 31 The gas temporarily stays in the inner space surrounded by the edge of the culture vessel 8 and the chamber cover 31, and escapes through the gap between the edge of the culture vessel 8 and the chamber cover 31, and the chamber housing ( Since it flows through the lower inlet of 31), it becomes possible to control and maintain the culture environment inside the culture vessel (8).

Although not shown in the drawing, in the chamber housing 31, an inlet (not shown in the drawing) for introducing the gas supplied from the environment control module 5 is formed at an appropriate position, and an inlet pipe (not shown in the drawing) is formed at the inlet. City) is connected.

In the culture chamber 3 formed as described above, the light irradiated from the light source block 64 to be described below passes through the inlet opened in the vertical direction of the chamber housing 31 in the vertical direction. Inside the culture chamber 3, the culture vessel 8 is accommodated to be positioned on an optical path through which light passes in the vertical direction.

The tensile stimulation module 4 is a configuration for repeatedly applying a linear tension and contraction force to the culture vessel 8 located inside the culture chamber 3. Referring to the drawings, the tension stimulation module 4 is composed of a tensioner block 41, a transfer screw 42, a driving motor 43, a jack slider 44 and a connector 45 are provided, and the It is coupled to the upper portion of the Y-direction transfer bed 22 at a position close to one side of the culture chamber 3.

The tensioner block 41 is a configuration for forming a structure in which the transfer screw 42 is supported by rotation, the driving motor is coupled, and the connector 45 is guided and supported in a straight line. Referring to the drawings, the tensioner block 41 is formed in a shape having an approximately rectangular rim, and is in a position close to one side of the chamber housing 31 of the culture chamber 3 in the Y-direction transfer bed ( It is fixedly coupled to the top of 22). A pair of support guides 411 are provided on both sides of the tensioner block 41 in the width direction facing each other to slide and support the connector 45 so as to move linearly.

The transfer screw 42 is a configuration for converting the rotational motion of the drive motor 43 into a linear motion together with the jack slider 44. Referring to the drawings, each of both ends of the transfer screw 42 is supported by a bearing or a bushing on each end wall in the front and rear direction of the tensioner block 41.

The drive motor 43 is configured to rotate the transfer screw 42 reciprocally and is fixed to one side of the transfer block 41 so that its rotation shaft is coupled to one end of the transfer screw 42. The reciprocating rotation of the drive motor 43 causes the transfer screw 42 to reciprocate, and accordingly, the jack slider 44 is linearly moved reciprocally along the transfer screw 42.

The jack slider 44 is configured to convert the rotational motion of the drive motor 43 into a linear motion together with the transfer screw 42. Referring to the drawings, the jack slider 44 is screwed to the transfer screw 42 to move linearly along the transfer screw 42 by rotation of the transfer screw 42.

The connector 45 is a configuration for connecting the jack slider 44 that is linearly moved and the culture vessel 8 located inside the culture chamber 3 to each other. Referring to the drawings, the connector 45 is positioned above the tensioner block 41 and supported by a support guide 411, and a slider connection 451 having a lower portion thereof coupled to the jack slider 44 And, it is composed of a vessel connecting portion 452, one end connected to the slider connection portion 451 and the other end connected to the culture vessel 8 located inside the culture chamber (3). The slider connection part 451 and the container connection part 452 constituting the connection body 45 are made of a rigid material that is less deformed, and are integrally combined with each other. Therefore, the linear motion of the jack slider 44 exerts a linear force on the rigid connector 45, and the force is transferred to the culture vessel 8 to provide a fine tensile force and contraction force to the culture vessel 8 This will be transmitted repeatedly.

The container connection part 452 of the connector 45 connects the slider connection part 451 located outside the culture chamber 3 and the culture container 8 located inside the culture chamber 3 to each other. For this purpose, it must be configured to be connected from the outside to the inside of the chamber housing 31 constituting the culture chamber 3. To this end, an embodiment in which the container connection part 452 is bent in a zigzag so as not to interfere with the chamber housing 31 and has a bent structure is shown in the drawing.

The ring connection control module 5 is a configuration for controlling and maintaining a culture environment such as temperature, humidity, gas concentration, etc. inside the culture chamber 3. The drawing shows a state in which a storage tank 51 in which gas and moisture are stored and mixed in order to control the concentration of gas such as temperature, humidity and carbon dioxide is coupled to the upper portion of the Y-direction transfer bed 22. . A heating means and a humidifying means are also provided to control temperature and humidity, and the atmospheric gas whose temperature, humidity, and gas concentration are controlled as described above is pumped by a pump 52 to connect the inlet pipe to the culture chamber 3. It is supplied to the culture chamber 3 through. On the other hand, in order to control the feedback of the culture environment according to the environmental condition inside the culture chamber 3, a sensor 53 for measuring the temperature, humidity, gas concentration and flow rate inside the culture chamber 3 controls the environment. It is provided in the module 53. The sensor 53 is installed in an appropriate position, such as the storage tank 51 or the inlet pipe (not shown in the drawing), and the inside of the culture chamber 3.

The microscope module 6 is a configuration for observing in real time a culture medium being cultured in a culture vessel 8 located inside the culture chamber 3. Meanwhile, the culture medium here refers to an organism or tissue that can be cultured in the culture vessel 8 such as cells, tissues, and microorganisms.

Referring to the drawings, the microscope module 6 includes a support block 61, an arm 62, a microscope 63 or a camera 63', and a light source block 64.

The support block 61 is fixed to the base 1 to support the arm 62. Referring to the drawings, the support block 61 is fixed to one edge of the frame 11 positioned above the base 1.

The arm 62 is a configuration for attaching and supporting the microscope 63 or the camera 63'. Referring to the drawings, the arm 62 is coupled to a support column 611 erected vertically to the support block 61 so as to move up and down. The arm 62 is moved vertically along the support column 611, that is, in the Z-direction, and is fixed so as not to move at an appropriate position. The microscope 63 or the camera 63' is attached to the tip of the arm 62.

The microscope 63 has the objective lens 631 in the vertical direction of the culture chamber 3 so that the culture medium cultivated in the culture vessel 8 located inside the culture chamber 3 can be observed visually. It is attached to the tip of the arm 62 so that it is located on the optical path through which the light passes.

Meanwhile, the camera 63 ′ is a configuration for photographing a culture material in real time and storing it as an image or an image, and is a configuration that is replaced with the microscope 63 or used simultaneously. Like the microscope 63, the camera 63' is attached to the tip of the arm 62 so as to be positioned on an optical path through which light passes in the vertical direction of the culture chamber 3. The image captured by the microscope 63 is transmitted to the control module 7 and stored or edited.

The light source block 64 is a component for irradiating light rays to the lower portion of the culture vessel 8 located inside the culture chamber 3. Referring to the drawings, the light source block 64 is coupled to and fixed to a block fixing bar 111 provided in the frame 11 so as to be positioned under the culture chamber 3.

8 is a detailed perspective view of the light source block 64. Referring to the drawing, the light source block 64 is composed of a block body 641, a filter slider 642, and a backlight (not shown in the drawing). do.

The block body 641 is configured to form a structure in which the backlight is accommodated and mounted therein, and the filter slider 642 is mounted in a sliding manner. The block body 641 has a structure in which a light irradiation hole 641a is formed upward, and a sliding hole 641b orthogonal to the light irradiation hole 641a is formed on the side. The backlight is accommodated and mounted inside the block body 641 so as to project light through the light irradiation hole 641a. The block body 641 is coupled to and fixed to the block fixing bar 111 provided in the frame 11.

The filter slider 642 has a configuration in which a plurality of filters 642a are integrally provided so that the plurality of filters 642a can be easily changed so that the sliding holes 641b can be slidably mounted. Referring to the drawings, the filter slider 642 is formed of a plate-shaped member inserted into the sliding hole 641b, and has a structure in which three filter holes 642b are arranged in a row in a longitudinal direction. Filters 642a for adjusting the wavelength or phase of light are installed in the three filter holes 642b.

The control module 7 controls the operation of the tensile stimulation module 4 for applying tensile stimulation to the culture vessel 8 and the environmental control module 5 for controlling and maintaining the environment inside the culture chamber 3 It is a configuration to do. For example, the control module 7 controls the operation of the driving motor 43 to adjust the intensity or frequency of the tensile stimulus applied to the culture vessel 8. In addition, the control module 7 calculates a physical quantity that needs to be adjusted from the temperature, humidity and gas concentration measured by the sensor 53, and accordingly, the humidification means operates the heating means and the means for mixing the gas. And it controls the operation of the pump for adjusting the flow rate.

In addition, the control module 7 may be configured to manage and edit the operation of the camera 63 ′ of the microscope module 6 and an image or image captured by the camera 63 ′.

The tension stimulation and real-time observation of the culture incubator system described above and shown in the drawings is only one example for carrying out the present invention, and should not be construed as limiting the technical idea of the present invention. The scope of protection of the present invention is determined only by the matters described in the following claims, and the improved and modified embodiments without departing from the gist of the present invention will be apparent to those of ordinary skill in the technical field to which the present invention belongs. As long as it will be said to belong to the protection scope of the present invention.

1 base
11 frames
111 block fixing bar
12 guide plate
13 X-direction straight guide
14 linear slider
2 Transfer bed module
21 X-direction transfer bed
211 opening
212 Y-direction straight guide
22 Y-direction transfer bed
3 culture chamber
31 chamber housing
32 chamber cover
4 Tension stimulation module
41 tensioner block
411 support guide
42 Transfer screw
43 Drive motor
44 Jack Slider
45 connector
451 slider connection
452 vessel connection
5 Environment control module
51 storage tank
52 pump
53 sensor
6 Microscope module
61 Support block
611 Support pillar
62 arm
63,63' microscope, camera
631 objective lens
64 Light source block
7 Control module
8 Culture container

Claims (5)

A culture chamber in which the culture vessel is accommodated so that the culture vessel is positioned on the optical path through which light passes in the vertical direction and the light passes in the vertical direction;
An environment control module for controlling and maintaining the temperature, humidity and gas atmosphere inside the culture chamber, a tensile stimulation module connected to one edge of the culture vessel to repeatedly apply a linear tension and contraction force to the culture vessel, and the culture A microscope module is provided with an objective lens or camera positioned on the optical path through which light passes in the vertical direction of the chamber to observe the culture medium cultured in the culture vessel, and a bed transferred in the XY direction orthogonal on the plane of the upper part of the base. The transferred bed module is included,
The culture chamber is supported by the transfer bed module and can be moved in the XY direction,
The culture chamber is configured to include a chamber housing opened up and down, and a chamber cover made of a material through which light is transmitted and coupled to an upper entrance of the chamber housing to cover the upper entrance of the chamber housing,
The tension stimulation module includes a tensioner block coupled to an upper portion of the transfer bed module, a transfer screw rotatably supported by the tensioner block, and a driving motor connected to the transfer screw to reciprocate the transfer screw. And, a jack slider that is moved along the transfer screw by rotation of the transfer screw, and is formed of a rigid body, and one end is connected to the jack slider located outside the chamber housing, and the other end is located inside the chamber housing. A culture incubator system capable of tensile stimulation and real-time observation, characterized in that comprising a connector connected to the edge of the culture vessel. The method of claim 1,
The microscope module includes a support block fixed to the base, an arm supported by the support block and moved up and down, and an optical path through which light passes in the vertical direction of the culture chamber. A light source block fixed to the base is provided and configured to irradiate,
The objective lens or the camera is coupled to the tip of the arm and positioned on an optical path through which light passes in the vertical direction of the culture chamber. A culture incubator system capable of tensile stimulation and real-time observation. The method of claim 2,
The light source block includes a block body in which a light irradiation hole is formed upward and a sliding hole orthogonal to the light irradiation hole is formed on a side thereof;
A filter slider formed of a plate-shaped member inserted into the sliding hole, a plurality of filters arranged in a row in the longitudinal direction, and mounted in the sliding hole to enable sliding movement;
A culture incubator system capable of tensile stimulation and real-time observation, characterized in that a backlight accommodated and mounted inside the block body is provided to project light through the light irradiation hole. delete delete

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