KR20020006498A - An Incuvator for Culture The Cell - Google Patents
An Incuvator for Culture The Cell Download PDFInfo
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- KR20020006498A KR20020006498A KR1020010062725A KR20010062725A KR20020006498A KR 20020006498 A KR20020006498 A KR 20020006498A KR 1020010062725 A KR1020010062725 A KR 1020010062725A KR 20010062725 A KR20010062725 A KR 20010062725A KR 20020006498 A KR20020006498 A KR 20020006498A
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Abstract
Description
본 발명은 세포 배양용 인큐베이터에 관한 것으로서 이는 특히, 챔버를 갖는 본체의 상측에 밀폐공간을 갖도록 투명재질의 커버가 장착되며, 밀폐공간의 내측에서 일정 배양조건을 만족하도록 제어기가 설치되고, 상기 챔버의 내측에 복수의 현미경을 장착하는 구성으로 세포의 조작, 검사, 배양작업을 하나의 챔버 내측에서 각각 수행하여 세포가 외부의 세균등에 의해 오염되는 것을 미연에 방지토록 하는 세포 배양용 인큐베이터에 관한 것이다.The present invention relates to an incubator for cell culture, which is particularly equipped with a cover made of a transparent material to have a sealed space on the upper side of the body having a chamber, and a controller is installed to satisfy a predetermined culture condition inside the sealed space, and the chamber The present invention relates to an incubator for cell culture in which a plurality of microscopes are mounted inside the cell, and each cell operation, inspection, and culture operation is performed inside one chamber to prevent the cell from being contaminated by external bacteria. .
일반적으로 인큐베이터는, 외부의 공기유입이 최소화 되도록 형성되는 챔버의 내측에 일정 환경을 조성하는 온도및 습도등을 선택적으로 조절하도록 되고, 상기 챔버의 내측에서 미생물이나 세포등을 배양하거나 미숙아의 생장에 사용하도록 된다.In general, the incubator is to selectively control the temperature and humidity, such as to create a certain environment inside the chamber is formed so as to minimize the external air inflow, and to incubate microorganisms or cells inside the chamber or to grow the premature infants Will be used.
또한, 상기 인큐베이터를 미생물의 배양에 사용하는 경우, 배양용기에 장착되는 배지의 상측에 미생물이 놓여져 챔버의 내측에 투입되는 일정습도와 온도에 의해 배양토록 된다.In addition, when the incubator is used for the cultivation of microorganisms, the microorganisms are placed on the upper side of the medium mounted on the culture vessel so as to be cultured by a constant humidity and temperature introduced into the chamber.
그리고, 상기 미생물의 배양전에 그 상태를 현미경을 통하여 검사하고, 미생물에 일정 조작작업을 수행한후 인큐베이터에 넣어 배양토록 한다.Then, the state of the microorganisms is examined through a microscope, and the microorganisms are subjected to a predetermined operation, and then put in an incubator to incubate.
이와같은 종래의 배양용 인큐베이터는 단순히 미생물의 배양에만 사용토록 되고, 그 구성은 도1에서와 같이, 내부에 일정용적의 챔버(24)를 갖는 본체(12)가 설치되어 그 전방에 챔버(24)를 밀폐토록 하면서 내부의 주시가 가능토록 투명재질의 내부도어(14)가 회전가능토록 연결되며, 상기 내부도어(14)의 일측에 본체(12)에 연결되어 내부도어(14)에 의해 밀폐되는 챔버(24) 긴밀하게 밀폐토록 외부도어(13)가 연결된다.Such a conventional culture incubator is to be used only for the cultivation of microorganisms, the configuration is as shown in Figure 1, the main body 12 having a predetermined volume of the chamber 24 is installed inside the chamber 24 in front of it The inner door 14 of the transparent material is rotatably connected so that the inside can be viewed while allowing the inside to be closed, and is connected to the main body 12 on one side of the inner door 14 to be sealed by the inner door 14. The outer door 13 is connected to the chamber 24 to be closely sealed.
또한, 상기 챔버(24)의 내측에 다단의 선반(29)이 적재되어 그 상측에 배양용기(11)가 적재되고, 상기 챔버(24)의 내측에 제어기(미도시)와 연결되는 다수의 관통홈및 팬(미도시)이 설치되어 제어기를 통하여 공급되는 열풍및 탄산가스등을 공급하여 미생물의 생육(生育)에 적합하도록 조정하고, 상기 챔버(24)의 저부에는 챔버내의 일정습도 유지를 위하여 물이 적재되는 수납함(38)이 설치되는 구성으로 이루어 진다.In addition, a plurality of shelves 29 are stacked inside the chamber 24 so that the culture vessel 11 is loaded thereon, and a plurality of through holes connected to a controller (not shown) inside the chamber 24. Grooves and fans (not shown) are installed to supply hot air and carbon dioxide gas supplied through a controller to adjust for growth of microorganisms, and to the bottom of the chamber 24 to maintain a constant humidity in the chamber. The stacked storage box 38 is configured to be installed.
그러나, 상기와 같은 인큐베이터는, 단순히 배양기로서의 역활만을 수행하여 별도의 검사및 조작을 위한 현미경이 필요하고, 미생물의 검사및 조작시 미생물이 접종되는 배양용기를 배양기의 외측에 분리하여 작업을 수행한후 이를 운반하여 배양기의 내측에 투입하여야 함으로써 이동중 배양용기에 세균이 침투할 염려가 있다.However, the incubator as described above simply performs a role as an incubator and requires a microscope for a separate inspection and manipulation, and separates the culture vessel in which the microorganism is inoculated during the inspection and manipulation of the microorganism to perform the work. Thereafter, there is a concern that bacteria may penetrate into the culture vessel during transportation by transporting it and putting it inside the culture vessel.
또한, 미생물의 검사나 조작을 위한 장치의 설치를 위한 별도의 공간을 필요로 하고, 도어의 잦은 개방에 의해 챔버내에 조건을 항상 일정하게 유지시킬수 없게 되는 단점이 있는 것이다.In addition, it requires a separate space for the installation of the device for the inspection or manipulation of the microorganisms, and the frequent opening of the door has the disadvantage that it is not always possible to keep the conditions constant in the chamber.
이를 개선하기 위한 본 발명의 목적은, 세포의 조작이나 검사및 배양을 동일 챔버의 내측에서 동시에 수행하도록 하고, 세포의 검사나 조작및 배양을 위한 각각의 장치를 하나로 장치로 집약시키도록 하는 세포 배양용 인큐베이터를 제공하는데 있다.An object of the present invention for improving this is to perform cell manipulation or inspection and culture at the same time inside the same chamber, and cell culture to aggregate each device into one device for inspection, manipulation and culture of cells. It is to provide an incubator for.
도1은 종래의 인큐베이터를 도시한 사시도1 is a perspective view showing a conventional incubator
도2는 본 발명에 따른 인큐베이터를 도시한 사시도Figure 2 is a perspective view of the incubator according to the present invention
도3은 본 발명에 따른 인큐베이터를 도시한 요부 단면도3 is a sectional view showing main parts of an incubator according to the present invention;
도4a,b는 각각 본 발명에 따른 현미경의 결합상태를 도시한 요부단면도Figures 4a, b is a cross-sectional view showing the main portion of the microscope coupled state according to the present invention, respectively
도5a,b는 각각 본 발명에 따른 조작부의 결합상태도및 도어의 분해도Figure 5a, b is an exploded view of the engaging state and the door of the operation unit according to the present invention, respectively
도6은 본 발명의 다른 실시예에 따른 인큐베이터를 도시한 사시도Figure 6 is a perspective view of an incubator according to another embodiment of the present invention
*도면의 주요 부분에 대한 부호의 설명** Description of the symbols for the main parts of the drawings *
100...본체 130...챔버100 ... body 130 ... chamber
140...지지플레이트 200...도어140 ... support plate 200 ... door
300...현미경 400...조작부300 ... microscope 400 ... control panel
430...밀봉시트430 ... Sealed Sheet
상기 목적들을 달성하기 위해 본 발명은, 상부면에 일정 챔버를 갖도록 형성되는 본체;The present invention to achieve the above object, the main body is formed to have a predetermined chamber on the upper surface;
상기 챔버에 밀폐공간을 형성토록 본체의 상부에 연결되며, 일측에 배양용기를 투입및 배출토록 도어가 설치되는 커버;A cover that is connected to an upper portion of the main body to form a sealed space in the chamber, and a door is installed to input and discharge the culture vessel to one side;
상기 챔버의 내측에 장착되는 플레이트와 상기 플레이트의 상부에 연결되는 대물 대안렌즈로 구성되며, 대안렌즈의 일측이 커버의 외측에 돌출토록 되고, 상기 챔버의 내측에 적어도 하나이상 장착되는 현미경;A microscope mounted to the inside of the chamber and an objective alternative lens connected to the upper portion of the plate, one side of the alternative lens protruding to the outside of the cover, and a microscope mounted to the inside of the chamber;
상기 현미경및 배양용기를 조작토록 커버에 관통공을 형성하여 장착되는 조작부; 및,An operation unit mounted to form a through hole in the cover to operate the microscope and the culture vessel; And,
온도조절기와 습도조절기및 탄산농도조절기에 연결되어 챔버 내측에 일정조건을 유지하도록 설치되는 제어부를 포함하여 구성되는 세포 배양용 인큐베이터를 제공한다.It provides a cell culture incubator comprising a control unit is connected to the temperature controller, humidity controller and carbonic acid concentration controller is installed to maintain a predetermined condition inside the chamber.
이하, 첨부된 도면에 의거하여 본 발명의 실시예를 상세하게 설명하면 다음과 같다.Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.
도1 내지 도5에서 도시한 바와같이 본 발명은, 상부면에 배양용기(120)가 장착되는 일정용적의 챔버(110)를 갖으면서 단열구조로 형성되는 본체(100)가 설치된다.As shown in Figures 1 to 5, the present invention is provided with a main body 100 formed in an insulating structure with a chamber 110 having a predetermined volume on which the culture vessel 120 is mounted.
또한, 상기 챔버(110)의 내측에 밀폐공간을 형성토록 본체(100)의 상부에 커버(200)가 연결되며, 상기 커버(200)의 일측에 배양용기(210)를 투입및 배출토록 도어(220)가 연결된다.In addition, the cover 200 is connected to the upper portion of the main body 100 to form a sealed space inside the chamber 110, the door to the inlet and discharge the culture vessel 210 on one side of the cover (200) 220 is connected.
상기 도어(220)는, 상기 커버(200)에 도어(220)가 장착토록 형성되는관통홈(220a)의 주연에 고정턱(220b)이 연장형성되어 그 내측에 실링부재(220c)가 삽입되며, 상기 도어(220)의 일측에 커버(200)의 걸림편(200a)에 지지되는 걸림고리(220d)가 연결된다.The door 220 has a fixing jaw (220b) is formed on the periphery of the through hole (220a) is formed so that the door 220 is mounted on the cover 200, the sealing member 220c is inserted therein On one side of the door 220, a locking ring 220d supported by the locking piece 200a of the cover 200 is connected.
그리고, 상기 챔버(110)의 내측에 현미경(300)이 장착되고, 상기 현미경(300)은, 검사및 조작을 위한 배양용기(210)가 놓여지면서 일정광원을 형성토록 내측에 램프가 내장되는 플레이트(300b)와 상기 플레이트(300b)의 상부에 연결되는 대물(330c)및 대안렌즈(330d)로 구성되며, 상기 대안렌즈(330d)의 일측이 커버(200)의 외측면에 돌출토록 장착되고, 상기 현미경(300)은, 챔버(110)의 내측에 적어도 하나이상 장착된다.In addition, the microscope 300 is mounted inside the chamber 110, and the microscope 300 includes a plate in which a lamp is embedded to form a constant light source while the culture vessel 210 is placed for inspection and manipulation. 300b and an object 330c connected to the upper portion of the plate 300b and an alternative lens 330d, one side of the alternative lens 330d is mounted to protrude to an outer surface of the cover 200, At least one microscope 300 is mounted inside the chamber 110.
또한, 상기 현미경(300)의 플레이트(300b) 상면은 챔버(110)의 저면과 동일평면을 갖도록 챔버(110)의 내면에 제2관통공(320)을 형성하여 이에 지지되면서 함몰 형성되는 실링부재(330)를 개재하여 밀착 고정되며, 상기 현미경(300)의 상부는 커버(200)의 일측에 형성되는 제1관통공(410)에 제1밀봉수단(350)을 통하여 연결된다.In addition, the upper surface of the plate 300b of the microscope 300 is formed with a second through hole 320 in the inner surface of the chamber 110 to have the same plane and the bottom surface of the chamber 110, the sealing member is formed recessed while It is tightly fixed through the 330, the upper portion of the microscope 300 is connected to the first through-hole 410 formed on one side of the cover 200 through the first sealing means 350.
계속하여, 상기 현미경(300)의 조작이 가능토록 커버(200)의 일면에 조작부(400)가 설치되고, 상기 조작부(400)는, 상기 커버(200)에 관통형성되는 하나이상의 제3관통공(420)에 제2밀봉수단(450)을 통하여 조작용 장갑(460)이 고정된다.Subsequently, an operation unit 400 is installed on one surface of the cover 200 so that the operation of the microscope 300 can be performed, and the operation unit 400 includes one or more third through holes formed through the cover 200. The operation glove 460 is fixed to the second sealing means 450 by 420.
또한, 상기 챔버(110)의 내측에 투입되는 공기의 온도와 습도및 탄산가스의 투입량을 조절하도록 공기,습도,탄산가스공급배관과 연결되는 조절기(500)가 설치되고, 이때 상기 조절기(500)는 챔버(110)의 내측에 설치되는 감지센서(미도시)와 전기적으로 연결된다.In addition, a regulator 500 is connected to the air, humidity, carbonic acid gas supply pipe to adjust the temperature and humidity of the air introduced into the chamber 110 and the amount of carbon dioxide gas, wherein the regulator 500 Is electrically connected to a sensing sensor (not shown) installed inside the chamber 110.
그리고, 상기 제1밀봉수단(350)은, 상기 현미경(300)의 상부면에 밀봉되는 커버지(350a)와 상기 제2관통공(410)의 주연에 돌출되는 고정턱(350d)및 상기 고정턱(350d)의 외측에 밀착되는 고정커버(350c)로서 구성된다.And, the first sealing means 350, the fixing jaw (350d) and the fixing protruding to the periphery of the cover 350a and the second through hole 410 is sealed to the upper surface of the microscope 300 It is comprised as the fixed cover 350c in close contact with the outer side of the jaw 350d.
또한, 상기 제2밀봉수단(450)은, 상기 제3관통공(420)의 주연에 돌출 형성되는 고정턱(450b)및 상기 고정턱(450b)의 외측에 밀착되는 고정커버(450c)로서 구성된다.In addition, the second sealing means 450 is configured as a fixing jaw 450b protruding from the periphery of the third through hole 420 and a fixing cover 450c in close contact with the outside of the fixing jaw 450b. do.
이와같은 구성으로 이루어진 본 발명의 작용을 설명하면 다음과 같다.Referring to the operation of the present invention made of such a configuration as follows.
도1 내지 도5에서와 같이, 일정용적의 챔버(110)를 갖으면서 단열구조를 갖도록 형성되는 본체(100)의 상부에 커버(200)가 연결될때 그 내측에 일정 밀폐공간을 형성함으로써 챔버(110)의 내측에 삽입되는 배양용기(210)에 항상 일정한 환경을 제공하도록 한다.1 to 5, when the cover 200 is connected to the upper portion of the main body 100 is formed to have a heat insulating structure while having a certain volume of the chamber (110) by forming a certain sealed space inside the chamber ( 110 to always provide a constant environment to the culture vessel 210 is inserted into the inside.
이때, 상기 배양용기(210)에서 배양되는 세포는 챔버(110)가 항상 일정조건을 만족시키도록 그 내측에 온도,습도및 이탄화탄소등의 농도가 조절기(500)를 통하여 조절토록 됨으로써 외부의 환경에 상관없이 배양정도가 항상 일정하게 유지되어 사용자에게 신뢰성을 제공한다.At this time, the cells cultured in the culture vessel 210, the temperature of the temperature, humidity and carbon dioxide inside such that the chamber 110 always meets a predetermined condition to be controlled through the controller 500 to the outside environment Regardless of the culture, the degree of incubation is always kept constant, providing the user with reliability.
또한, 상기 배양용기(210)는, 챔버(110)를 덮는 커버(200)의 일측에 개폐토록 설치되는 도어(220)에 의해 배양용기(210)의 투입및 배출토록이 용이하게 이루아 진다.In addition, the culture vessel 210, the door 220 is installed to open and close on one side of the cover 200 to cover the chamber 110 is made easy to insert and discharge the culture vessel 210.
이때, 상기 도어(220)는, 그 일측에 연결되는 걸림고리(220d)가 커버(200)의 걸림편(200a)에 지지될때 상기 도어(220)가 고정턱(220b)에 밀착홈(미도시)을 통하여 지지되는 실링부재(220c)를 압입함으로써 이를 통한 외부공기의 유입이 방지토록 된다.At this time, the door 220, the door 220 is in close contact with the fixing jaw (220b) when the engaging ring (220d) connected to one side thereof is supported by the locking piece (200a) of the cover 200 (not shown) By pressing the sealing member (220c) is supported through) to prevent the inflow of external air through it.
그리고, 상기 챔버(110)의 내측에 대안렌즈(330d)만이 커버(200)의 외부로 돌출토록 되는 현미경(300)이 장착되고, 상기 현미경(300)에 의해 일정조건을 갖는 챔버(110)의 내측에서 배양되는 세포의 조작및 검사가 가능하여 이동에 따른 세균등의 검염을 완벽하게 방지토록 한다.In addition, a microscope 300 is mounted inside the chamber 110 such that only the alternative lens 330d protrudes out of the cover 200, and the microscope 110 of the chamber 110 has a predetermined condition. Manipulation and inspection of cells cultured from the inside is possible, so as to completely prevent infections such as bacteria due to migration.
또한, 상기 현미경(300)의 조작은 커버(200)의 일측에 설치되는 조작부(400)에 의해 용이하게 조작토록 된다.In addition, the operation of the microscope 300 is easily manipulated by the operation unit 400 installed on one side of the cover 200.
상기 조작부(400)는, 상기 커버(200)에 관통형성되는 하나이상의 제3관통공(420)에 제2밀봉수단(450)을 통하여 고정되는 조작용 장갑(460)으로 구성되어 현미경과 배양용기등의 조작이 손쉽게 이루어 진다.The operation unit 400 is composed of an operating glove 460 fixed to the one or more third through holes 420 formed through the cover 200 through the second sealing means 450, the microscope and the culture vessel. The operation of the light is made easily.
이때, 상기 조작용 장갑(460)은 각각의 손가락이 삽입토록 되어 미세조작이 가능토록 된다.At this time, the operation glove 460 is such that each finger is inserted so that fine operation is possible.
또한, 상기 현미경(300)은, 챔버(110)의 내측에 적어도 하나이상 장착되어 여러 사람이 동시에 배양되는 세포등을 검사하거나 조작 가능토록 된다.In addition, the microscope 300 is equipped with at least one inside the chamber 110 to be able to inspect or manipulate cells, such as several cultures at the same time.
그리고, 상기 현미경(300)는, 챔버(110)의 내측 저면에 제2관통공(320)을 형성한후 이에 고정되는 실링부재(330)를 개재하여 밀착 고정되어 플레이트(300b) 상면과 챔버(110)의 저면이 동일평면을 갖도록 됨으로써 배양용기등의 이동에 따라사용이 간편하게 이루어 진다.Then, the microscope 300, the second through-hole 320 is formed on the inner bottom surface of the chamber 110 and tightly fixed through the sealing member 330 fixed thereto, the upper surface of the plate 300b and the chamber ( The bottom of the 110) is to have the same plane, the use is made easy according to the movement of the culture vessel.
계속하여, 상기 본체(100)는, 조절기(500)가 설치되어 챔버(110)의 내측에 투입되는 공기의 온도와 습도및 탄산가스의 량을 조절토록 하고, 상기 챔버(110)의 내측에는 감지센서(미도시)가 설치되어 공급되는 탄산가스등의 농도를 감지하여 조절기(500)에 전기신호를 전달함으로써 챔버내의 조건을 항상 일정하게 유지하도록 한다.Subsequently, the main body 100 is provided with a regulator 500 to adjust the temperature and humidity of the air introduced into the chamber 110 and the amount of carbon dioxide gas, the sensing inside the chamber 110 A sensor (not shown) is installed to sense the concentration of carbon dioxide gas and the like to be supplied to transmit the electrical signal to the controller 500 so that the conditions in the chamber are always kept constant.
상기와 같이 챔버(110)의 내측에 일정조건으로 배양되는 배양용기(210) 내측의 세포를 도어를 개방하지 않는 상태에서 현미경(300)을 통하여 조작및 검사 가능토록 하여 배양용기(210)에 항상 일정한 조건을 유지시켜 정확한 결과를 얻을수 있도록 한다.As described above, the cells inside the culture vessel 210 cultured in a predetermined condition inside the chamber 110 can be manipulated and inspected through the microscope 300 in a state in which the door is not opened. Maintain constant conditions to ensure accurate results.
또한, 상기 챔버(110)의 내측에서 현미경(300)을 분리한후 대안렌즈가 삽입토록 되는 제1관통공(410)과 플레이트(300b)가 삽입되는 제2관통공(320)을 각각 별도의 밀폐판(미도시)에 의해 밀폐하면 일반적인 인큐베이터와 동일한 동작이 가능토록 되는 것이다.In addition, after the microscope 300 is separated from the inside of the chamber 110, the first through hole 410 into which the alternative lens is inserted and the second through hole 320 into which the plate 300b is inserted are separately provided. Sealing by a sealing plate (not shown) is to enable the same operation as a general incubator.
이와같이 본 발명의 세포 배양용 인큐베이터에 의하면, 세포의 조작이나 검사및 배양이 동일 챔버의 내측에서 동시에 수행되어 배양용기의 이동에 따른 오염물의 유입이나 감염이 방지되고, 세포의 검사나 조작및 배양을 위한 각각의 장치를 하나로 장치로 집약하여 설치공간의 제약이 최소화 되며, 정정인 상태에서 세포의 조작이나 검사작업이 수행되어 작업성을 극대화 시키도록 하는 것이다.Thus, according to the cell culture incubator of the present invention, the operation, inspection and cultivation of the cells is carried out at the same time inside the same chamber to prevent the inflow or infection of contaminants due to the movement of the culture vessel, and to examine, manipulate and culture the cells. By concentrating each device into one device, the limitation of installation space is minimized, and the operation or inspection work of cells is performed in the correct state to maximize workability.
또한, 2인 이상이 동시에 세포의 조작이나 검사등의 수행할수 있으며, 외기의 유입을 최소화 하여 배양기의 신뢰성을 향상시키도록 하고, 단순한 배양기로서의 사용이 가능한 효과가 있는 것이다.In addition, two or more people can simultaneously perform manipulation and inspection of cells, and minimize the inflow of outside air to improve the reliability of the incubator, it is possible to use as a simple incubator.
본 발명은 특정한 실시예에 관련하여 도시하고 설명 하였지만, 이하의 특허청구범위에 의해 제공되는 본 발명의 정신이나 분야를 벗어나지 않는 한도내에서 본 발명이 다양하게 개량 및 변화될수 있다는 것을 당업계에서 통상의 지식을 가진자는 용이하게 알수 있음을 밝혀 두고자 한다.While the invention has been shown and described with respect to specific embodiments thereof, it will be apparent to those skilled in the art that various changes and modifications can be made without departing from the spirit or scope of the invention as provided by the following claims. I would like to clarify that those who have knowledge of this can easily know.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR100700503B1 (en) * | 2006-06-08 | 2007-03-28 | 김영래 | Device for experiment and observation on cell |
| KR100960106B1 (en) * | 2008-01-21 | 2010-05-27 | 전남대학교산학협력단 | Incubator having multiple cell culture system |
| CN104178420A (en) * | 2014-09-05 | 2014-12-03 | 中国科学技术大学 | Living cell culture environment control system for microscope |
| CN104237236A (en) * | 2014-09-26 | 2014-12-24 | 中国水产科学研究院黑龙江水产研究所 | Assistant researching device for feeding habits of freshwater fishes |
| KR20200062476A (en) * | 2018-11-27 | 2020-06-04 | 김철 | Culture incubator system capable of real-time observating and tensile stimulating culture |
| CN116463217A (en) * | 2023-05-23 | 2023-07-21 | 太平洋康泰科学仪器（济南）有限公司 | Humidification incubator and use method thereof |
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| KR101706153B1 (en) | 2015-07-31 | 2017-02-14 | 한국과학기술원 | Microfluidic chip-based cell cultivation system |
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| KR100199697B1 (en) * | 1995-06-14 | 1999-06-15 | 윤경주, 김재석 | Environment cultivating apparatus |
| JP3378709B2 (en) * | 1995-10-13 | 2003-02-17 | 三洋電機株式会社 | Incubator |
| US5792427A (en) * | 1996-02-09 | 1998-08-11 | Forma Scientific, Inc. | Controlled atmosphere incubator |
| JP3670876B2 (en) * | 1998-09-29 | 2005-07-13 | 三洋電機株式会社 | Incubator |
| US6297047B1 (en) * | 1999-08-25 | 2001-10-02 | Spx Corporation | Ultraviolet sterilization of CO2 cell-culture incubator internal environments |
| KR200208443Y1 (en) * | 2000-06-13 | 2001-01-15 | 변홍무 | Carbon Dioxide Culture Unit Using PH Determine Ability |
| KR200236207Y1 (en) * | 2001-02-13 | 2001-10-25 | 주식회사 토이랩 | Portable microbe cultivator |
| KR200271563Y1 (en) * | 2001-11-30 | 2002-04-11 | 양남웅 | Incubator of glove box type |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100700503B1 (en) * | 2006-06-08 | 2007-03-28 | 김영래 | Device for experiment and observation on cell |
| KR100960106B1 (en) * | 2008-01-21 | 2010-05-27 | 전남대학교산학협력단 | Incubator having multiple cell culture system |
| CN104178420A (en) * | 2014-09-05 | 2014-12-03 | 中国科学技术大学 | Living cell culture environment control system for microscope |
| CN104178420B (en) * | 2014-09-05 | 2016-04-13 | 中国科学技术大学 | A kind of microscope viable cell culture environment Controlling System |
| CN104237236A (en) * | 2014-09-26 | 2014-12-24 | 中国水产科学研究院黑龙江水产研究所 | Assistant researching device for feeding habits of freshwater fishes |
| KR20200062476A (en) * | 2018-11-27 | 2020-06-04 | 김철 | Culture incubator system capable of real-time observating and tensile stimulating culture |
| CN116463217A (en) * | 2023-05-23 | 2023-07-21 | 太平洋康泰科学仪器（济南）有限公司 | Humidification incubator and use method thereof |
| CN116676176A (en) * | 2023-06-06 | 2023-09-01 | 湖南格致生物科技有限公司 | Cell bank mycoplasma detection equipment and detection method thereof |
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