CN116676176A - Cell bank mycoplasma detection equipment and detection method thereof - Google Patents
Cell bank mycoplasma detection equipment and detection method thereof Download PDFInfo
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- CN116676176A CN116676176A CN202310663441.9A CN202310663441A CN116676176A CN 116676176 A CN116676176 A CN 116676176A CN 202310663441 A CN202310663441 A CN 202310663441A CN 116676176 A CN116676176 A CN 116676176A
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- 238000001514 detection method Methods 0.000 title claims abstract description 41
- 241000204031 Mycoplasma Species 0.000 title claims abstract description 22
- 239000011521 glass Substances 0.000 claims abstract description 16
- 230000001681 protective effect Effects 0.000 claims description 27
- 230000001954 sterilising effect Effects 0.000 claims description 27
- 238000004659 sterilization and disinfection Methods 0.000 claims description 24
- 238000007789 sealing Methods 0.000 claims description 10
- 230000001012 protector Effects 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 2
- 241000204003 Mycoplasmatales Species 0.000 claims 6
- 239000000428 dust Substances 0.000 abstract description 14
- 239000012535 impurity Substances 0.000 abstract description 11
- 238000012360 testing method Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 10
- 230000006978 adaptation Effects 0.000 description 6
- 238000013461 design Methods 0.000 description 5
- 238000004891 communication Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000011324 bead Substances 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000012865 aseptic processing Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/35—Mycoplasma
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The application belongs to the technical field of mycoplasma detection equipment, in particular to a cell bank mycoplasma detection equipment, which aims at the problems that a microscope lens on the existing equipment is easy to be infected by dust and impurities in the air, so that the lens and a glass slide are polluted, and a test article is easy to damage, and the problem that the microscope lens cannot be detected is solved. The application also provides a detection method of the cell bank mycoplasma detection device. Set up protection and portable mechanism on the equipment body to and fixed subassembly, conveniently remove the equipment body, can carry out the closing cap with the microscope on the equipment body, reduce dust and impurity in the air and invade the microscope lens, prevent that the equipment body from empting and rocking and leading to the safety cover to drop.
Description
Technical Field
The application relates to the technical field of mycoplasma detection equipment, in particular to cell bank mycoplasma detection equipment and a detection method thereof.
Background
Mycoplasma is the smallest microorganism that survives extracellularly, a prokaryotic cell type of microorganism lacking a cell wall, is highly polymorphic, and has a variety of forms such as sphere, rod, wire, branch, etc. Unlike bacteria and also unlike viruses, mycoplasma can only adhere to receptors on the surface of the epithelial cells of the respiratory or genitourinary tract, without entering the tissues and blood.
Through retrieval, bulletin number CN215250802U discloses a mycoplasma rapid detection device, which comprises a constant-temperature sterilization culture cabinet, wherein a sterilizing lamp is arranged on the upper inner wall of the constant-temperature sterilization culture cabinet, an observation groove is arranged on the upper surface of the constant-temperature sterilization culture cabinet, a microscope is fixedly connected to the upper surface of the constant-temperature sterilization culture cabinet, a sealing port is arranged on the right surface of the constant-temperature sterilization culture cabinet, threads are arranged on the inner wall of the sealing port, a fixing frame is fixedly connected to the right inner wall of the constant-temperature sterilization culture cabinet, a sliding groove is arranged on the surface of the fixing frame, and a rapid detection mechanism is arranged on the inner wall of the sealing port; the rapid detection mechanism comprises a sealing column, a placing rack is fixedly connected to the left surface of the rotating block, a sliding block is fixedly connected to the front of the placing rack, and a culture dish placing plate is fixedly connected to the inner wall of the placing rack; the front face of the constant temperature sterilization culture cabinet is provided with the observation mechanism, a plurality of groups of culture dishes can be placed at one time for culture, the detection efficiency is effectively improved, the operation is simple, the phenomenon of improper operation is avoided, and the possibility of culture failure is reduced.
However, the technical scheme has the following problems: the microscope lens on the equipment is easy to be infected by dust and impurities in the air, so that the lens and a glass slide are polluted, undetectable, inconvenient to operate and easy to damage a test article when the equipment needs to be moved.
In view of the above problems, the present document proposes a mycoplasma cell library detection apparatus for solving the above-mentioned problems.
Disclosure of Invention
The application provides a cell bank mycoplasma detection device, which solves the defects that a microscope lens on the device is easy to be infected by dust and impurities in the air, so that the lens and a glass slide are polluted and cannot be detected, and when the device needs to be moved, the operation is inconvenient and a test product is easy to damage in the prior art.
The application provides the following technical scheme:
a cell bank mycoplasma detection device, comprising:
the device comprises a device body and two transparent glasses, wherein the front side and the rear side of the device body are respectively provided with a placing groove, the inner walls of the two placing grooves are fixedly connected with the outer sides of the periphery of the transparent glasses, and the two transparent glasses are respectively provided with a manual operation hole for operation;
the protection mechanism is arranged on the equipment body and used for protecting the equipment body;
the portable mechanism is arranged at the top of the equipment body and is used for conveniently moving the equipment body;
the fixing component is arranged on the protection mechanism and used for fixing the protection mechanism.
In one possible design, the protection mechanism includes two safety covers and two draw-in grooves, the top both sides of equipment body all are provided with the microscope that is used for observing, two the draw-in groove all sets up the top both sides at equipment body, two the draw-in groove is located the microscope outside, the size of draw-in groove and the size looks adaptation of safety cover, the top inner wall height of safety cover is higher than the microscope height.
In one possible design, the portable mechanism includes two handles and two rotation posts, two the bottom of rotation post respectively with the top both sides rotation of equipment body are connected, two the rotation post is the cross with the safety cover and arranges, two the rotation post respectively with two landing leg fixed connection of handle.
In a possible design, fixed subassembly includes four fixed columns, four connecting blocks and four fixed orifices, four the fixed orifices all sets up at the top of equipment body, four the fixed orifices distributes respectively in the both sides of draw-in groove, the both sides of safety cover all with one side fixed connection of connecting block, four all seted up the round hole with the fixed orifices looks adaptation on the connecting block, the one end of fixed column runs through the round hole and extends to the bottom inner wall of fixed orifices, fixed column and fixed orifices threaded connection, the other end fixedly connected with column cap of fixed column, the diameter of column cap is greater than the round hole.
In one possible design, two hanging plates are fixedly connected to one side of the equipment body, the hanging plates are obliquely arranged, and the size of each hanging plate is smaller than that of the protective cover.
In one possible design, the four corners of the bottom of the device body are fixedly connected with corner protectors, and the bottoms of the four corner protectors are fixedly connected with rubber pads.
Based on the same conception, the application also provides a detection method of the cell bank mycoplasma detection device, which comprises the following steps:
step one, placing an equipment body on a flat seat surface, and opening a protective cover on a hanging plate on one side of the equipment body when a microscope is required to be used;
step two, detecting by using the equipment body, placing a culture dish with a cover in the equipment body on the lower inner wall of a culture dish placing plate, then inserting the placing frame into a constant-temperature sterilization culture cabinet through a sealing port, pushing the sliding block surface to the inner wall of a sliding chute of a fixing frame in a sleeved mode leftwards, driving the surface of a sealing column to rotate in a threaded mode on the inner wall of the sealing port by rotating a handle, isolating the constant-temperature sterilization culture cabinet from the external environment, performing aseptic treatment on the constant-temperature sterilization culture cabinet by a sterilizing lamp of the constant-temperature sterilization culture cabinet,
thirdly, placing a plurality of groups of culture dishes at one time for culturing;
and fourthly, after the culture is finished, a detector observes the culture dish in the constant-temperature sterilization culture cabinet through an observation window, then inserts a hand into the manual operation hole and places the culture dish on the lower inner wall of the detection table with the glove fixedly connected with the hand, and the microscope detects and observes the cultured culture medium, so that the detection work is finished in the constant-temperature sterilization culture cabinet.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application as claimed.
According to the application, the angle bead and the rubber pad are arranged at the bottom of the equipment body, so that the equipment body is far away from the seat surface after being placed, water stains and dust on the seat surface are prevented from polluting the equipment body, the equipment body is guaranteed to be clean and tidy, the equipment body is buffered to a certain extent when the equipment body is collided and extruded, and the protective cover can be opened on the hanging plate arranged at one side of the equipment body when a microscope is needed to be used, so that the protective cover can be quickly found when the protective cover is needed to be used, the waste in time is reduced, and the loss of the protective cover is prevented;
according to the application, the protection and portable mechanism and the fixing component are arranged on the equipment body, so that the equipment body is convenient to move when the equipment body is moved, the equipment body does not need to be held or lifted when the equipment body is moved, the labor amount is reduced, accidents are prevented from happening when the equipment body is not used, a microscope on the equipment body can be covered, dust and impurities in the air are reduced to infect the microscope, the microscope is prevented from being stained with the dust and impurities, the undetectable result is caused, the clamping groove is arranged to ensure that the protective cover can be conveniently placed on the microscope, the protective cover can be fixed, the falling of the protective cover caused by the falling and shaking of the equipment body is prevented, and the equipment body can be protected to a certain extent when the equipment body is overturned.
Drawings
FIG. 1 is a schematic diagram of a front view of a Mycoplasma cell wall detection device according to an embodiment of the present application;
fig. 2 is a schematic bottom view of a mycoplasma cell library detection apparatus according to an embodiment of the present application;
FIG. 3 is an exploded view of a top-down side-view structure of a Mycoplasma cell-library detection device according to an embodiment of the present application;
fig. 4 is a schematic diagram of a local enlarged structure of a mycoplasma cell library detection device according to an embodiment of the present application.
Reference numerals:
1. an equipment body; 2. a protective cover; 3. a connecting block; 4. fixing the column; 5. a handle; 6. a spin column; 7. transparent glass; 8. corner protection; 9. a placement groove; 10. a rubber pad; 11. a hanging plate; 12. a clamping groove; 13. and a fixing hole.
Description of the embodiments
Embodiments of the present application will be described below with reference to the accompanying drawings in the embodiments of the present application.
In describing embodiments of the present application, it should be noted that, unless explicitly stated and limited otherwise, the terms "coupled" and "mounted" should be interpreted broadly, and for example, "coupled" may or may not be detachably coupled; may be directly connected or indirectly connected through an intermediate medium. In addition, "communication" may be direct communication or may be indirect communication through an intermediary. Wherein, "fixed" means that the relative positional relationship is not changed after being connected to each other. References to orientation terms, such as "inner", "outer", "top", "bottom", etc., in the embodiments of the present application are merely to refer to the orientation of the drawings and, therefore, the use of orientation terms is intended to better and more clearly illustrate and understand the embodiments of the present application, rather than to indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be construed as limiting the embodiments of the present application.
In embodiments of the present application, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature.
In the embodiment of the present application, "and/or" is merely an association relationship describing an association object, and indicates that three relationships may exist, for example, a and/or B may indicate: a exists alone, A and B exist together, and B exists alone. In addition, the character "/" herein generally indicates that the front and rear associated objects are an "or" relationship.
Reference in the specification to "one embodiment" or "some embodiments" or the like means that a particular feature, structure, or characteristic described in connection with the embodiment is included in one or more embodiments of the application. Thus, appearances of the phrases "in one embodiment," "in some embodiments," "in other embodiments," and the like in the specification are not necessarily all referring to the same embodiment, but mean "one or more but not all embodiments" unless expressly specified otherwise. The terms "comprising," "including," "having," and variations thereof mean "including but not limited to," unless expressly specified otherwise.
Examples
Referring to FIGS. 1-4, a cell bank mycoplasma detection apparatus comprising:
the device comprises a device body 1 and two transparent glasses 7, wherein the front side and the rear side of the device body 1 are respectively provided with a placing groove 9, the inner walls of the two placing grooves 9 are fixedly connected with the outer sides of the periphery of the transparent glasses 7, and the two transparent glasses 7 are respectively provided with a manual operation hole for operation;
the protection mechanism is arranged on the equipment body 1 and used for protecting the equipment body 1;
the portable mechanism is arranged at the top of the equipment body 1 and is used for conveniently moving the equipment body 1;
the fixing component is arranged on the protection mechanism and used for fixing the protection mechanism.
Set up protection and portable mechanism on equipment body 1 to and fixed subassembly for when removing equipment body 1, conveniently remove equipment body 1, when moving equipment body 1, do not need to hold or lift equipment body 1, reduce the amount of labour, prevent unexpected when removing, also make when not using equipment body 1, can carry out the closing cap with the microscope on the equipment body 1, reduce dust and impurity in the air and infect the microscope, prevent to be stained with the microscope and blur, cause the effect that can't detect, set up draw-in groove 12 and guarantee that safety cover 2 can conveniently place on the microscope, can fix safety cover 2, prevent equipment body 1 from empting and rocking and lead to safety cover 2 to drop, and can carry out certain protection to equipment body 1 when equipment body 1 is tipped over.
Referring to fig. 1 and 3, the protection mechanism includes two safety cover 2 and two draw-in grooves 12, and the top both sides of equipment body 1 all are provided with the microscope that is used for observing, and two draw-in grooves 12 all set up the top both sides at equipment body 1, and two draw-in grooves 12 are located the microscope outside, and the size of draw-in groove 12 and the size looks adaptation of safety cover 2, and the top inner wall height of safety cover 2 is higher than the microscope height.
The microscope on the equipment body 1 is provided with the protection cover 2, and the equipment body 1 is provided with the clamping groove 12 for placing the protection cover 2, so that when the equipment body 1 is not used, the microscope on the equipment body 1 can be covered, dust and impurities in air are reduced to infect the microscope, the microscope is prevented from being stained with blurred, the undetectable result is caused, and the clamping groove 12 is provided to ensure that the protection cover 2 can be conveniently placed on the microscope.
Referring to fig. 1, 3 and 4, the portable mechanism includes two handles 5 and two rotating columns 6, the bottoms of the two rotating columns 6 are respectively connected with two sides of the top of the device body 1 in a rotating manner, the two rotating columns 6 are arranged in a cross manner with the protective cover 2, and the two rotating columns 6 are respectively fixedly connected with two supporting legs of the handles 5.
Pulling handle 5, then erects handle 5 through the column 6 that rotates that sets up on equipment body 1, and rethread handle 5 removes equipment body 1 to suitable place for when removing equipment body 1, conveniently remove equipment body 1 like this, when removing equipment body 1, need not hold or lift equipment body 1, reduce the amount of labour, also prevent to appear unexpected when removing, then place equipment body 1 on flat seating surface.
Referring to fig. 1 and 3, the fixed subassembly includes four fixed columns 4, four connecting blocks 3 and four fixed orifices 13, four fixed orifices 13 all set up at the top of equipment body 1, four fixed orifices 13 distribute respectively in the both sides of draw-in groove 12, the both sides of safety cover 2 all with one side fixed connection of connecting block 3, the round hole with the fixed orifice 13 looks adaptation has all been seted up on four connecting blocks 3, the one end of fixed column 4 runs through the round hole and extends to the bottom inner wall of fixed orifice 13, fixed column 4 and fixed orifice 13 threaded connection, the other end fixedly connected with cap of fixed column 4, the diameter of cap is greater than the round hole.
The protection cover 2 sets up connecting block 3 and fixed column 4 all around, can fix protection cover 2, prevents that equipment body 1 from empting and rocking and lead to protection cover 2 to drop to can carry out certain protection to equipment body 1 when equipment body 1 tumbles.
The application can be used in the technical field of mycoplasma detection equipment and can also be used in other fields suitable for the application.
Examples
Referring to FIGS. 1-4, a cell bank mycoplasma detection apparatus comprising:
the device comprises a device body 1 and two transparent glasses 7, wherein the front side and the rear side of the device body 1 are respectively provided with a placing groove 9, the inner walls of the two placing grooves 9 are fixedly connected with the outer sides of the periphery of the transparent glasses 7, and the two transparent glasses 7 are respectively provided with a manual operation hole for operation;
the protection mechanism is arranged on the equipment body 1 and used for protecting the equipment body 1;
the portable mechanism is arranged at the top of the equipment body 1 and is used for conveniently moving the equipment body 1;
the fixing component is arranged on the protection mechanism and used for fixing the protection mechanism.
Set up protection and portable mechanism on equipment body 1 to and fixed subassembly for when removing equipment body 1, conveniently remove equipment body 1, when moving equipment body 1, do not need to hold or lift equipment body 1, reduce the amount of labour, prevent unexpected when removing, also make when not using equipment body 1, can carry out the closing cap with the microscope on the equipment body 1, reduce dust and impurity in the air and infect the microscope, prevent to be stained with the microscope and blur, cause the effect that can't detect, set up draw-in groove 12 and guarantee that safety cover 2 can conveniently place on the microscope, can fix safety cover 2, prevent equipment body 1 from empting and rocking and lead to safety cover 2 to drop, and can carry out certain protection to equipment body 1 when equipment body 1 is tipped over.
Referring to fig. 1 and 3, the protection mechanism includes two safety cover 2 and two draw-in grooves 12, and the top both sides of equipment body 1 all are provided with the microscope that is used for observing, and two draw-in grooves 12 all set up the top both sides at equipment body 1, and two draw-in grooves 12 are located the microscope outside, and the size of draw-in groove 12 and the size looks adaptation of safety cover 2, and the top inner wall height of safety cover 2 is higher than the microscope height.
The microscope on the equipment body 1 is provided with the protection cover 2, and the equipment body 1 is provided with the clamping groove 12 for placing the protection cover 2, so that when the equipment body 1 is not used, the microscope on the equipment body 1 can be covered, dust and impurities in air are reduced to infect the microscope, the microscope is prevented from being stained with blurred, the undetectable result is caused, and the clamping groove 12 is provided to ensure that the protection cover 2 can be conveniently placed on the microscope.
Referring to fig. 1, 3 and 4, the portable mechanism includes two handles 5 and two rotating columns 6, the bottoms of the two rotating columns 6 are respectively connected with two sides of the top of the device body 1 in a rotating manner, the two rotating columns 6 are arranged in a cross manner with the protective cover 2, and the two rotating columns 6 are respectively fixedly connected with two supporting legs of the handles 5.
Pulling handle 5, then erects handle 5 through the column 6 that rotates that sets up on equipment body 1, and rethread handle 5 removes equipment body 1 to suitable place for when removing equipment body 1, conveniently remove equipment body 1 like this, when removing equipment body 1, need not hold or lift equipment body 1, reduce the amount of labour, also prevent to appear unexpected when removing, then place equipment body 1 on flat seating surface.
Referring to fig. 1 and 3, the fixed subassembly includes four fixed columns 4, four connecting blocks 3 and four fixed orifices 13, four fixed orifices 13 all set up at the top of equipment body 1, four fixed orifices 13 distribute respectively in the both sides of draw-in groove 12, the both sides of safety cover 2 all with one side fixed connection of connecting block 3, the round hole with the fixed orifice 13 looks adaptation has all been seted up on four connecting blocks 3, the one end of fixed column 4 runs through the round hole and extends to the bottom inner wall of fixed orifice 13, fixed column 4 and fixed orifice 13 threaded connection, the other end fixedly connected with cap of fixed column 4, the diameter of cap is greater than the round hole.
The protection cover 2 sets up connecting block 3 and fixed column 4 all around, can fix protection cover 2, prevents that equipment body 1 from empting and rocking and lead to protection cover 2 to drop to can carry out certain protection to equipment body 1 when equipment body 1 tumbles.
Referring to fig. 1 and 2, two hanging plates 11 are fixedly connected to one side of the apparatus body 1, the hanging plates 11 are obliquely arranged, and the size of the hanging plates 11 is smaller than that of the protection cover 2.
When the microscope is needed to be used, the protective cover 2 can be opened and placed on the hanging plate 11 on one side of the equipment body 1, so that the protective cover 2 can be quickly found when the protective cover 2 is needed to be used, the waste in time is reduced, and the loss of the protective cover 2 is prevented.
Referring to fig. 1-3, four corners of the bottom of the device body 1 are fixedly connected with corner protectors 8, and the bottoms of the four corner protectors 8 are fixedly connected with rubber pads 10.
The angle bead 8 and the rubber pad 10 that set up in equipment body 1 bottom for equipment body 1 is placing the back, is keeping away from the seat, prevents that the water stain and the dust on the seat from being infected with equipment body 1, guarantees the clean and tidy of equipment body 1, and when equipment body 1 receives collision and extrusion, has certain cushioning effect to equipment body 1.
The working principle and the using flow of the technical scheme are as follows: when the device is specifically used, the handle 5 is pulled firstly, then the handle 5 is erected through the rotating column 6 arranged on the device body 1, and then the device body 1 is moved to a proper place through the handle 5, so that the device body 1 is convenient to move when the device body 1 is moved, the device body 1 does not need to be held or lifted when the device body 1 is moved, the labor is reduced, accidents are prevented when the device body 1 is moved, then the device body 1 is placed on a flat seat surface, the device body 1 is far away from the seat surface after being placed due to the corner protector 8 and the rubber pad 10 arranged at the bottom of the device body 1, water stains and dust on the seat surface are prevented from being stained with the device body 1, the cleanness of the device body 1 is ensured, the device body 1 is buffered to a certain extent when the device body 1 is collided and extruded, the protection cover 2 is arranged on a microscope on the device body 1, and a clamping groove 12 for placing the protective cover 2 is arranged on the equipment body 1, so that when the equipment body 1 is not used, a microscope on the equipment body 1 can be covered, dust and impurities in air are reduced to infect the microscope lens, the microscope lens is prevented from being stained with and blurred, the undetectable result is caused, the clamping groove 12 is arranged to ensure that the protective cover 2 can be conveniently placed on the microscope, a connecting block 3 and a fixing column 4 are arranged around the protective cover 2, the protective cover 2 can be fixed, the protective cover 2 is prevented from falling down due to the toppling and shaking of the equipment body 1, the equipment body 1 can be protected to a certain extent when the equipment body 1 is overturned, the protective cover 2 can be opened and placed on a hanging plate 11 on one side of the equipment body 1 when the microscope is required to be used, when the protective cover 2 is required to be used, can find safety cover 2 fast, reduce the waste in the time, and prevent loss of safety cover 2, when needs use equipment body 1 to detect, can use the area lid culture dish in the equipment body 1 to place at the culture dish and place the inboard wall under the board, then insert the rack through the seal mouth in the constant temperature sterilization culture cabinet with the slide groove inner wall that the slide block surface cup jointed to the mount promote left, then rotate the hand (hold) and drive the seal post surface at seal mouth inner wall screw thread rotation, make constant temperature sterilization culture cabinet and external environment keep apart, then carry out aseptic processing by the sterilamp of constant temperature sterilization culture cabinet to it, once can place the multiunit culture dish and cultivate culture dish, effectually improved detection efficiency, observe the culture dish in the constant temperature sterilization culture cabinet through the observation window after accomplishing the cultivation, then insert its fixedly connected gloves in the manual operation hole with the culture dish and place under the inspection bench inner wall, then detect the culture medium that finishes by the microscope, make the detection work can accomplish inside the constant temperature sterilization culture cabinet. The apparatus body 1 is described in detail in the bulletin number CN215250802U, and thus, a detailed description thereof will not be given here.
The present application is not limited to the above embodiments, and any person skilled in the art can easily think about the changes or substitutions within the technical scope of the present application, and the changes or substitutions are intended to be covered by the scope of the present application; embodiments of the application and features of the embodiments may be combined with each other without conflict. Therefore, the protection scope of the application is subject to the protection scope of the claims.
Claims (7)
1. A cell pool mycoplasma detection apparatus, comprising:
the device comprises a device body (1) and two transparent glasses (7), wherein the front side and the rear side of the device body (1) are respectively provided with a placing groove (9), the inner walls of the two placing grooves (9) are respectively fixedly connected with the outer sides of the periphery of the transparent glasses (7), and the two transparent glasses (7) are respectively provided with a manual operation hole for operation;
the protection mechanism is arranged on the equipment body (1) and used for protecting the equipment body (1);
the portable mechanism is arranged at the top of the equipment body (1) and is used for conveniently moving the equipment body (1);
the fixing component is arranged on the protection mechanism and used for fixing the protection mechanism.
2. The mycoplasma cell bank detection apparatus as claimed in claim 1, wherein the protection mechanism comprises two protection covers (2) and two clamping grooves (12), microscopes for observation are respectively arranged on two sides of the top of the apparatus body (1), two clamping grooves (12) are positioned on the outer sides of the microscopes, the size of each clamping groove (12) is matched with the size of the protection cover (2), and the height of the inner wall of the top of the protection cover (2) is higher than that of the microscope.
3. The mycoplasma cell bank detection device according to claim 1, wherein the portable mechanism comprises two handles (5) and two rotating columns (6), the bottoms of the two rotating columns (6) are respectively and rotatably connected with two sides of the top of the device body (1), the two rotating columns (6) are arranged in a cross shape with the protective cover (2), and the two rotating columns (6) are respectively and fixedly connected with two supporting legs of the handles (5).
4. The mycoplasma cell bank detection device according to any one of claims 1-3, wherein the fixing assembly comprises four fixing columns (4), four connecting blocks (3) and four fixing holes (13), the four fixing holes (13) are all arranged at the top of the device body (1), the four fixing holes (13) are respectively distributed at two sides of the clamping groove (12), two sides of the protective cover (2) are fixedly connected with one side of the connecting blocks (3), round holes matched with the fixing holes (13) are respectively formed in the four connecting blocks (3), one ends of the fixing columns (4) penetrate through the round holes and extend to the bottom inner wall of the fixing holes (13), the fixing columns (4) are in threaded connection with the fixing holes (13), column caps are fixedly connected to the other ends of the fixing columns (4), and the diameters of the column caps are larger than the round holes.
5. The mycoplasma cell library detection device according to claim 1, wherein two hanging plates (11) are fixedly connected to one side of the device body (1), the hanging plates (11) are obliquely arranged, and the size of the hanging plates (11) is smaller than that of the protective cover (2).
6. The mycoplasma cell bank detection device according to claim 1, wherein four corners of the bottom of the device body (1) are fixedly connected with corner protectors (8), and rubber pads (10) are fixedly connected to bottoms of the four corner protectors (8).
7. A detection method of the mycoplasma cell pool detection apparatus as defined in claim 1 to 6, comprising the steps of:
step one, placing the equipment body (1) on a flat seat surface, and opening and placing the protective cover (2) on a hanging plate (11) on one side of the equipment body (1) when a microscope is required to be used;
step two, detecting by using the equipment body (1), placing a culture dish with a cover in the equipment body (1) on the lower inner wall of a culture dish placing plate, then inserting the placing frame into a constant-temperature sterilization culture cabinet through a sealing port, pushing the sliding block surface to the inner wall of a sliding chute of a fixed frame in a sleeved mode leftwards, driving the surface of a sealing column to rotate in a threaded mode on the inner wall of the sealing port by rotating a handle, isolating the constant-temperature sterilization culture cabinet from the external environment, performing aseptic treatment on the constant-temperature sterilization culture cabinet by a sterilizing lamp of the constant-temperature sterilization culture cabinet,
thirdly, placing a plurality of groups of culture dishes at one time for culturing;
and fourthly, after the culture is finished, a detector observes the culture dish in the constant-temperature sterilization culture cabinet through an observation window, then inserts a hand into the manual operation hole and places the culture dish on the lower inner wall of the detection table with the glove fixedly connected with the hand, and the microscope detects and observes the cultured culture medium, so that the detection work is finished in the constant-temperature sterilization culture cabinet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310663441.9A CN116676176A (en) | 2023-06-06 | 2023-06-06 | Cell bank mycoplasma detection equipment and detection method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310663441.9A CN116676176A (en) | 2023-06-06 | 2023-06-06 | Cell bank mycoplasma detection equipment and detection method thereof |
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| CN116676176A true CN116676176A (en) | 2023-09-01 |
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| CN202310663441.9A Pending CN116676176A (en) | 2023-06-06 | 2023-06-06 | Cell bank mycoplasma detection equipment and detection method thereof |
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