CN220745899U - Aseptic culture box and aseptic carbon dioxide incubator for partitioned culture - Google Patents
Aseptic culture box and aseptic carbon dioxide incubator for partitioned culture Download PDFInfo
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- CN220745899U CN220745899U CN202321967657.6U CN202321967657U CN220745899U CN 220745899 U CN220745899 U CN 220745899U CN 202321967657 U CN202321967657 U CN 202321967657U CN 220745899 U CN220745899 U CN 220745899U
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- sterile
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- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 title claims abstract description 44
- 229910002092 carbon dioxide Inorganic materials 0.000 title claims abstract description 22
- 239000001569 carbon dioxide Substances 0.000 title claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000012528 membrane Substances 0.000 claims abstract description 17
- 238000007789 sealing Methods 0.000 claims abstract description 14
- 238000010438 heat treatment Methods 0.000 claims abstract description 12
- 238000005192 partition Methods 0.000 claims description 18
- 238000009833 condensation Methods 0.000 claims description 4
- 230000005494 condensation Effects 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 6
- 239000007789 gas Substances 0.000 description 10
- 238000004113 cell culture Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000013316 zoning Methods 0.000 description 1
Abstract
The utility model discloses a sterile culture box and a sterile carbon dioxide incubator for partitioned culture, wherein the sterile culture box comprises an integrally formed box body, the box body comprises an opening and a box cover for selectively opening and closing the opening, and a culture cavity for placing cells is arranged in the box body. An incubator, comprising: a case; the upper part and the lower part of the air duct plate are respectively provided with an air inlet communicated with the accommodating cavity and an air outlet communicated with the accommodating cavity; a blower; a water tray and a heating device. The incubator can be divided into two or more areas through structural adjustment, and a sealed sterile box is arranged in each area and performs gas exchange with uniform air in the cavity through filter membranes at two sides; in the use process, the cell taking and placing is operated by only opening a specified aseptic box sealing cover, and other cells without operation are still cultured in an independent aseptic box. Meanwhile, the air in the box body and the water vapor are forced to be convected by a fan and an air duct which are specially designed.
Description
Technical Field
The utility model relates to the technical field of aseptic culture, in particular to an aseptic culture box and an aseptic carbon dioxide incubator for partitioned culture.
Background
The carbon dioxide incubator is formed by simulating a growth environment similar to cells or tissues in organisms in an incubator body, and the incubator requires stable temperature (37 ℃) and stable CO 2 The device for culturing cells or tissues in vitro is an advanced instrument for culturing cells, tissues and microorganisms, and is a key device necessary for developing immunology, oncology, genetics and bioengineering.
For example, in the utility model patent application number CN202220787120.0 (publication number CN 217230701U) of the applicant's prior application, a condensing plate is disposed in a steam chamber formed by surrounding a water tray and a guide cover, and because the steam formed in the water tray passes through the condensing plate and then is liquefied and condensed, the temperature of the steam entering the culture chamber is lower than the internal temperature of the culture chamber, and thus, the steam cannot be condensed on the inner wall of the culture chamber and a storage rack to form condensed water.
However, in the above incubator, cells are not subjected to the zoning culture, and the cells are liable to affect each other, which results in contamination of the cells.
Disclosure of Invention
The present utility model aims to provide a sterile culture cassette and a sterile carbon dioxide incubator for zone culture, which solve one or more of the above-mentioned problems of the prior art.
On the one hand, the sterile culture box comprises an integrally formed box body, wherein the box body comprises an opening and a box cover for selectively opening and closing the opening, a culture cavity for placing cells is formed in the box body, a shelf is slidably arranged at the lower end of the box body, one end of the shelf is fixedly connected with the lower end of the box cover, air holes are respectively formed in two sides of the box body, and HEPA filter membrane plates are arranged in the air holes.
In some embodiments, a sealing bevel is provided at the opening of the case body, and a sealing ring is provided between the sealing bevel and the case cover.
In some embodiments, the box further comprises an elastic locking piece, wherein the elastic locking piece is arranged on one of the box cover and the box body and is clamped with the other one, and the elastic locking piece is used for limiting the box cover to be separated from the box body.
In another aspect, a zone-cultured sterile carbon dioxide incubator according to the present utility model comprises:
the box body is internally provided with a containing cavity;
the air duct plate is covered on the top wall, the rear side wall and the bottom wall in the box body, an air duct is formed by surrounding the air duct plate and the wall surfaces, and the upper part and the lower part of the air duct plate are respectively provided with an air inlet communicated with the accommodating cavity and an air outlet communicated with the accommodating cavity;
the fan is arranged at the top of the air duct and can convey air flow from the inlet of the air duct to the outlet of the air duct;
the water tray is formed by downwards sinking the bottom wall in the box body, and a steam cavity is formed between the water tray and the air duct plate in a surrounding mode;
and the heating device is used for heating the box body and the water tray.
In some embodiments, further comprising:
at least one cavity partition board capable of being drawn into and out of the accommodating cavity and dividing the accommodating cavity into two or more areas;
and at least two sterile culture cassettes placed on the cavity partition.
In some embodiments, a guide plate is arranged between the cavity partition plate and the water tray, a condensation plate and a drain pipe are arranged below the water tray, the condensation plate is in heat conduction connection with the water tray, and the drain pipe is communicated with the steam cavity.
In some embodiments, a HEPA filter is also included, the HEPA filter covering at the air intake.
In some embodiments, the heating device is disposed at the top, bottom, left side, right side, front side, and rear side of the case.
In some embodiments, a temperature sensor and a gas concentration sensor are disposed within the housing.
In some embodiments, the case includes an outer shell and an inner cavity with a space between the inner cavity and the outer shell, a glass door hinged to a front side of the inner cavity, and a door hinged to a front side of the outer shell.
The beneficial effects are that:
1. the sterile culture box adopts high-temperature-resistant transparent plastic, the front of the box body is a door cover with a sealing ring and an elastic locking piece, HEPA (high efficiency) filter membrane plates (the filter precision is 0.2 mu m, the efficiency is more than or equal to 99.99%) are hermetically sealed at two sides of the box body, only air and water vapor can pass through the HEPA filter membrane plates, the filter membrane is made of hydrophobic materials under the sterile condition, other parts except the filter membrane can be sterilized at high temperature and high pressure, and therefore the sterile state of the whole box body in use is ensured.
2. The sterile carbon dioxide incubator for zone culture performs forced convection on air and water vapor in the incubator body through a specially designed fan and an air duct; an air inlet is arranged in the cavity, and the input gas and air are mixed and circularly flow in the box body, so that the uniformity of air in the box body is ensured, and the stability of the cell culture environment is ensured.
3. The sterile carbon dioxide incubator for zone culture can be used for dry heat culture (anhydrous) and wet heat culture (watery) according to user requirements, and different gases can be input (such as by input)
CO 2 pH value of gas regulating environment and input O 2 Or N 2 Conditioned air O 2 Concentration, etc.) to meet the user's needs; through intelligent control of software and cooperation with corresponding sensor feedback, the environment in the box body can be ensured to be stable, cells are required to be taken and put to switch the door in the use process in time,
under the intelligent adjustment of software, the required environment can be recovered rapidly, and the culture is stable.
4. The inside of the cavity of the sterile carbon dioxide incubator for the subarea culture can be divided into two or more areas through structural adjustment, and a sealed sterile box is placed in the subarea and performs gas exchange with uniform air in the cavity through filter membranes at two sides; in the use process, the cell taking and placing is operated by only opening a specified aseptic box sealing cover, and other cells without operation are still cultured in an independent aseptic box; the cell is placed in the sterile box for the independent culture of the partition, the cell partition of the simultaneous incubator in the incubator is isolated, the air and the humidity are exchanged through the filtering membranes at the two sides, the independent culture effect is achieved, the cell culture process and the cell picking and placing process are guaranteed, the cells in different partitions are not affected mutually, the pollution of the cells is avoided, and the growth of the cells is effectively assisted.
Drawings
FIG. 1 is a schematic diagram showing the structure of a sterile culture cassette according to an embodiment of the present utility model;
FIG. 2 is a schematic structural view of a sterile culture cassette according to an embodiment of the present utility model;
FIG. 3 is a cross-sectional view of a sterile carbon dioxide incubator for zone culture in accordance with one embodiment of the present utility model;
FIG. 4 is a schematic diagram showing the structure of a sterile carbon dioxide incubator for zone-divided culture according to an embodiment of the present utility model;
FIG. 5 is a schematic view showing a part of a sterile carbon dioxide incubator for zone-divided culture according to an embodiment of the present utility model;
FIG. 6 is a schematic diagram showing a part of a sterile carbon dioxide incubator for zone-divided culture according to an embodiment of the present utility model.
Detailed Description
The present utility model will be described in further detail with reference to the accompanying drawings.
As shown in fig. 1 and 2:
the aseptic culture box comprises an integrally formed box body 11, wherein the box body 11 comprises an opening and a box cover 12 which is selectively opened and closed, a culture cavity for placing cells is formed in the box body 11, a shelf 13 is slidably arranged at the lower end of the inner part of the box body 11, one end of the shelf 13 is fixedly connected with the lower end of the box cover 12, ventilation holes are formed in two sides of the box body 11 respectively, HEPA filter membrane plates 14 are arranged in the ventilation holes, a sealing inclined plane is arranged at the opening of the box body 11, and a sealing ring is arranged between the sealing inclined plane and the box cover.
The box cover is also provided with an elastic locking piece 15, the elastic locking piece 15 is clamped with the box body 11, and the box cover 12 is limited to be separated from the box body 11 by controlling the elastic locking piece 15.
Specifically, the elastic locking piece 15 includes a locking knob, a locking piece and a clamping groove formed in the box body 11, and a worker can clamp the locking piece into the clamping groove by rotating the locking knob, so that the box cover 12 is limited to be separated from the box body 11.
The embodiment of the utility model adopts high-temperature resistant transparent plastic, the front surface of the box body is provided with a door cover with a sealing ring and an elastic locking piece, HEPA (high efficiency) filter membrane plates (the filter precision is 0.2 mu m, the efficiency is more than or equal to 99.99%) are hermetically sealed at two sides of the box body, only air and water vapor can pass through the HEPA filter membrane plates, the filter membrane is made of hydrophobic materials under aseptic conditions, and other parts except the filter membrane can be sterilized at high temperature and high pressure, so that the whole box body is ensured to be in an aseptic state when in use.
As shown in the figure 3 of the drawings,
a sterile carbon dioxide incubator for zonal culture comprises
A case 20 having an accommodating chamber 21 formed therein;
the air duct board 30 is covered on the top wall, the rear side wall and the bottom wall in the box body 20, and an air duct 31 is formed by surrounding the air duct board 30 and the wall surfaces, and the upper part and the lower part of the air duct board 30 are respectively provided with an air inlet communicated with the accommodating cavity and an air outlet communicated with the accommodating cavity;
a fan 40, wherein the fan 40 is installed at the top of the air duct 31, and can convey air flow from the inlet of the air duct 31 to the outlet of the air duct 31;
the water tray 50 is formed by downwards sinking the bottom wall in the box 20, a steam cavity is formed between the water tray 50 and the air duct plate 30 in a surrounding manner, a guide plate 90 is arranged between the cavity partition plate 60 and the water tray 50, a guide groove 91 is formed in the middle of the guide plate 90, a condensing plate 51 and a drain pipe 52 are arranged below the water tray 50, the condensing plate 51 is in heat conduction connection with the water tray 50, and the drain pipe 52 is communicated with the steam cavity;
a HEPA filter 70, the HEPA filter 70 covering at the air intake;
and heating means for heating the case 20 and the water tray 50.
According to the embodiment of the utility model, the fan and the air duct are specially designed, so that forced convection is carried out on air and water vapor in the box body; an air inlet is arranged in the cavity, and the input gas and air are mixed and circularly flow in the box body, so that the uniformity of the air in the box body is ensured, and the stability of the cell culture environment is ensured; the purpose of the guiding groove is to guide wind to the middle of the aseptic box, so that air flow and mixing in the middle of the box body are more effectively promoted, and uniformity and fluidity of air around the aseptic box are ensured, as shown in fig. 6.
In order to realize intelligent control, the heating device is arranged at the top, bottom, left side, right side, front side and rear side of the box 20, and a temperature sensor 81 and a gas concentration sensor 82 are arranged in the box 20.
According to the embodiment of the utility model, the six sides of the whole box body are provided with the heating wires with special designs, the temperature of the air in the cavity is raised through the heat radiation of the metal plate, and the air in the cavity can reach the temperature point set by a user under the forced convection system in the cavity by the intelligent control of software and matching with at least one temperature sensor in the cavity.
Meanwhile, dry heat culture (anhydrous) and wet heat culture (water) can be carried out according to the requirements of users, and different gases can be input (such as the pH value of the environment is adjusted by inputting CO2 gas, the concentration of O2 of air is adjusted by inputting O2 or N2, and the like) so as to achieve the environment required by the users; through the intelligent control of software, cooperation corresponding sensor feedback can guarantee that the interior environment of box is stable, in time need get and put the cell and switch the door in the use, under the intelligent adjustment of software, the environment of the needs of also can be quick resumption, stable cultivation.
As shown in figure 4 of the drawings,
the case 20 includes a housing 22 and an inner cavity 23, a space is provided between the inner cavity 23 and the housing 22, a glass door 24 is hinged to the front side of the inner cavity 23, and a door 25 is hinged to the front side of the housing 22.
The two cavity clapboards 60 in the box body 20 are respectively a first cavity clapboards 61 arranged below and a second cavity clapboards 62 arranged above, the cavity clapboards 60 can be pulled in and out of the accommodating cavity 21 and divide the accommodating cavity 21 into two areas, and two sterile culture boxes 10 are arranged on each cavity clapboards 60 side by side. As shown in fig. 5, in order to facilitate the insertion and removal of the fungus boxes, the first chamber partition 61 is provided with a first inclined guide plate 63, and the second chamber partition 62 is provided with a second guide plate 64 and a third guide plate 65 spaced apart.
The inside of the box body of the embodiment of the utility model can be divided into two or more areas through structural adjustment, and a sealed sterile box is arranged in the areas and performs gas exchange with uniform air in the box body through filter membranes at two sides; in the use process, the cell taking and placing is operated by only opening a specified aseptic box sealing cover, and other cells without operation are still cultured in an independent aseptic box; the cell is placed in the sterile box for the independent culture of the partition, the cell partition of the simultaneous incubator in the incubator is isolated, the air and the humidity are exchanged through the filtering membranes at the two sides, the independent culture effect is achieved, the cell culture process and the cell picking and placing process are guaranteed, the cells in different partitions are not affected mutually, the pollution of the cells is avoided, and the growth of the cells is effectively assisted.
The working principle of this embodiment is as follows:
when the device works, the heating device heats water in the water tray 50 to form steam, the steam in the steam cavity enters the accommodating cavity through the air outlet which is communicated with the accommodating cavity by the air duct plate 30, so that certain humidity is formed in the accommodating cavity, and the accommodating cavity is placed in an aseptic box which is independently cultivated in a partitioning way by the accommodating cavity partition plate 60 for cultivation, and air and humidity exchange is carried out through filtering membranes at two sides; the fan 40 sucks the damp and hot air flow in the accommodating cavity into the air duct 31 through the air inlet, and the air flow is mixed with the steam in the water tray 50 and then flows back to the accommodating cavity through the air outlet, and the air flow is continuously and circularly mixed under the driving of the fan 40, so that a stable and uniform air environment is formed in the accommodating cavity 1. Because the condensing plate 51 is externally connected with the water tray, the steam is liquefied and condensed after passing through the condensing plate 23, so that the temperature of the steam entering the accommodating cavity is lower than the temperature inside the accommodating cavity, and the steam cannot be condensed on the inner wall of the accommodating cavity and the cavity partition plate 60 to form condensed water.
The foregoing is merely a preferred embodiment of the present utility model, and it should be noted that a person skilled in the art may make several similar variations and modifications without departing from the inventive concept, which should also be considered as being within the scope of the present utility model.
Claims (9)
1. The sterile culture box is characterized by comprising a box body (11) which is integrally formed, wherein the box body (11) comprises an opening and a box cover (12) which is selectively opened and closed, a culture cavity for placing cells is formed in the box body (11), a shelf (13) is slidably arranged at the lower end inside the box body (11), one end of the shelf (13) is fixedly connected with the lower end of the box cover (12), ventilation holes are formed in two sides of the box body (11) respectively, and HEPA filter membrane plates (14) are arranged in the ventilation holes.
2. The sterile culture cassette according to claim 1, wherein a sealing bevel is provided at the opening of the cassette body (11), and a sealing ring is provided between the sealing bevel and the cassette cover.
3. The sterile incubator of claim 1, further comprising an elastic locking element (15), wherein the elastic locking element (15) is disposed on one of the lid (12) and the case body (11) and is engaged with the other, and the elastic locking element (15) is used for limiting the lid (12) from being separated from the case body (11).
4. Sterile carbon dioxide incubator of subregion cultivation, its characterized in that includes:
a box body (20) with a containing cavity (21) formed therein;
the air duct plate (30) is covered on the top wall, the rear side wall and the bottom wall in the box body (20), an air duct (31) is formed by surrounding the air duct plate and the wall surfaces, and the upper part and the lower part of the air duct plate (30) are respectively provided with an air inlet communicated with the accommodating cavity and an air outlet communicated with the accommodating cavity;
the fan (40) is arranged at the top of the air duct (31) and can convey air flow from the inlet of the air duct (31) to the outlet of the air duct (31);
the water tray (50) is formed by downwards sinking the bottom wall in the box body (20), and a steam cavity is formed between the water tray (50) and the air duct plate (30) in a surrounding mode;
the heating device is used for heating the box body (20) and the water tray (50);
at least one cavity partition (60), wherein the cavity partition (60) can be drawn in and out of the accommodating cavity (21) and divides the accommodating cavity (21) into two or more areas;
and at least two sterile culture cassettes (10) according to any one of claims 1-3, said sterile culture cassettes (10) being placed on said cavity partition (60).
5. The sterile carbon dioxide incubator for zoned culture according to claim 4, wherein a guide plate (90) is arranged between the cavity partition plate (60) and the water tray (50), a condensation plate (51) and a drain pipe (52) are arranged below the water tray (50), the condensation plate (51) is in heat conduction connection with the water tray (50), and the drain pipe (52) is communicated with the steam cavity.
6. The zoned-cultured sterile carbon dioxide incubator of claim 4, further comprising a HEPA filter (70), the HEPA filter (70) overlaying at the air intake.
7. The sterile carbon dioxide incubator for zone culture according to claim 4, wherein the heating means are arranged at the top, bottom, left side, right side, front side and rear side of the incubator body (20).
8. The sterile carbon dioxide incubator for zone culture according to claim 4, wherein a temperature sensor (81) and a gas concentration sensor (82) are provided in the case (20).
9. The sterile carbon dioxide incubator for zonal culture according to claim 4, wherein the case (20) comprises a housing (22) and an inner cavity (23), a space is provided between the inner cavity (23) and the housing (22), a glass door (24) is hinged to the front side of the inner cavity (23), and a door (25) is hinged to the front side of the housing (22).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321967657.6U CN220745899U (en) | 2023-07-25 | 2023-07-25 | Aseptic culture box and aseptic carbon dioxide incubator for partitioned culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321967657.6U CN220745899U (en) | 2023-07-25 | 2023-07-25 | Aseptic culture box and aseptic carbon dioxide incubator for partitioned culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220745899U true CN220745899U (en) | 2024-04-09 |
Family
ID=90564160
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321967657.6U Active CN220745899U (en) | 2023-07-25 | 2023-07-25 | Aseptic culture box and aseptic carbon dioxide incubator for partitioned culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220745899U (en) |
-
2023
- 2023-07-25 CN CN202321967657.6U patent/CN220745899U/en active Active
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