KR20200059902A - Cosmetic composition for improving the odor of the elderly using natural products - Google Patents

Abstract

The present invention relates to a cosmetic composition having an antibacterial effect and an effect of alleviating odor of elderly, and using natural products having no skin irritation. That is, the present invention provides the cosmetic composition which uses natural products containing a Xanthium sibiricum extract or a lemonglass oil extract as an active component, to have an effect of preventing oxidation, the antibacterial effect, the effect of alleviating odor of elderly, and an effect of inhibiting bad smell, and at the same time having no skin irritation.

Description

Cosmetic composition for improving the odor of the elderly using natural products {Cosmetic composition for improving the odor of the elderly using natural products}

The present invention relates to a cosmetic composition for improving senile odor using a natural product having antibacterial and anti-aging properties and no skin irritation.

Body odor, commonly referred to as senile odor, is a type of aging that occurs with age. It occurs mainly in the whole body, such as the armpits and groin, as it ages. It can be said to be one of the biggest concerns from the middle-aged to the old, as it not only makes them fall, but also significantly reduces the quality of life.

As the age increases, the hormone changes and metabolism are not smooth, and many waste products are produced. As a result, changes in the secretion of sweat glands and sebaceous glands make low-fat fatty acid <hexadisenoic acid> (9-hexadecenoic acid). It is secreted, and when nonenaldehyde (C9H16O) is formed as the unsaturated fatty acid is decomposed, it stinks. In addition, the secreted substance is known to produce senile odor due to the decrease in antioxidant capacity and activation of oxidation reactions by free radicals and microorganisms. As the activity of the elderly is increasing, the elderly are making various efforts to improve it. The area where the elderly smells badly is located in the back of the head, neck, chest, armpits, and back of the ears. It is known that the secretion of 9-nuclear decenoic acid is strong in the above areas. Therefore, there is a need to develop natural materials capable of removing the normal substance (Nonenal = C9H16O), which is the cause of senile body odor, and suppressing rot on the skin.

The cause of senile odor that develops with age is, first, the secretion of low-grade fat among components secreted due to a decrease in hormones and antioxidant capacity increases, and odor-like substances in the form of aldehydes are formed while continuously exposed to oxidation. , Anaerobics of microorganisms ( Staphylococcus epidermis (KCTC 1917)) Bacteria) produce odorous substances in decomposition products.

In addition, the pores generated by the expansion of pores due to skin aging may also cause odor by pores, bacteria, and the like.

In the present invention, as the sebum secretion containing 9-nuclear desenoic acid increases and becomes oxidized by being attached to free radicals and becomes lipid peroxide, the skin fungus eats lipid peroxide, and as it decomposes, the skin fungus excretes a substance containing 2-nonnal It can be said that there are a number of mechanisms that inhibit the odor of the elderly because it is mixed with sweat and body odor.

     On the other hand, Changija (Dokomari) is a fruit of Dokomari, a kind of medicinal plant, and is a representative plant inhabiting various parts of Korea. It is a first-year herbaceous plant that grows in diameter from 50 to 100 cm. Flowers running conically in August-September are yellow. The elliptical fruit, about 1 cm long, has hook-like projections and contains 2 aquatic fruits. It is attached to other objects with hook-like projections.

Tocomari is a medicine that removes the wind, and is used for sinusitis, rhinitis, headache, fever, cough, limb pain, paralysis, itching of the skin, and otitis media. The raised tortoise is a medicine that can be found in mountains or fields, and is known to be good for various skin diseases, and is actually used as a herbal medicine because it has the effect of eliminating itching and inflammatory reactions of the skin.

While the present inventors were exploring materials that can prevent or improve geriatric odor from natural resources in Korea, they confirmed for the first time that there was an effect to improve geriatric odor from Changja extract, a tortilla fruit, and completed a series of experiments. It was found to be a substance.

In addition, lemongrass, as its name suggests, is a lemon-scented herb. The leaves that resemble the grass of lemongrass tear apart and smell like lemon. The main ingredient of this fragrance is 'Citral' like lemon, which accounts for 70 to 80% of essential oil. Lemongrass essential oils are used in medicines, perfumes, soaps, rinses, and candies. It is also used for the treatment of abdominal pain, diarrhea, headache, and fever.

Lemongrass is easily grown in sandy soils in the midsummer. You can also use it as a potpourri or bath as a fragrance that dries the leaves to purify the room. It is grown in large quantities in Guatemala, Brazil, Madagascar, and Kagoshima in Japan for the purpose of collecting spices.

KR No. 10-0446114 KR No. 10-1221104 (Patent Publication No. 0003) KR No. 10-2009-0125588 (Patent Publication No. 0004) KR No. 10-2010-0129962 (Patent Publication No. 0005) KR No. 10-2013-0086395

An object of the present invention is to provide a cosmetic composition having an excellent odor improvement effect and skin irritation.

In addition, another object of the present invention is to provide a cosmetic composition for improving skin odor or improving senile odor using Changija extract and lemongrass oil.

The present invention is characterized by a cosmetic composition for improving elderly odor using a natural product containing Changija extract as an active ingredient.

Changija extract contains 0.001 to 10.0% by weight based on the total weight of the cosmetic composition.

Lemongrass oil extract is included in the Changja extract as an active ingredient.

With respect to the total weight of the cosmetic composition, the intestinal extract contains 0.01 to 10.0% by weight, and the lemongrass oil extract contains 0.01 to 0.001 to 10.0% by weight.

The Changja extract, a) extracting the Changja with ethanol to obtain an ethanol extract; And b) sequentially adding and fractionating hexane and ethyl acetate to the ethanol extract to obtain an extract.

 The step a) is performed at 40 to 100 ° C. for 5 to 48 hours, and the step b) includes the step of obtaining the acetic acid fraction by fractionating 1 to 5 times.

The cosmetic composition; Nutrient Cosmetics, Softening Cosmetics, Cream, Essence, Cleansing Foam, Cleansing Gel, Lotion, Powder, Ointment, Spray, Consil Stick, Solution, Gel, Solid, Suspension, Micro Emulsion, Micro Capsule, Micro Granules, Ionic Follicle Dispersant, Non It is characterized by a cosmetic composition for improving elderly odor using natural products formulated with one or more selected from the group consisting of ionic vesicle dispersants.

The cosmetic composition for improving senile odor using natural products according to the present invention, Changija extract inhibits the hexadecenoic acid, and the lemongrass extract suppresses secretions including fatty acids and fatty alcohols to have antibacterial and sensitizing effects and to stimulate skin irritation. It has the advantage that it can be used as a new cosmetic for improving old odor.

Hereinafter, an extract for improving odor of the present invention and a cosmetic composition comprising the same will be described in detail with reference to Examples. The following embodiments are provided as examples to ensure that the spirit of the present invention is sufficiently conveyed to those skilled in the art. Therefore, the present invention is not limited to the embodiments presented below and may be embodied in other forms. In addition, the same reference numbers throughout the specification indicate the same components.

The present invention relates to a cosmetic composition for improving senile odor using a natural product having antibacterial and anti-aging effects and having no skin irritation using Changija extract and lemongrass oil extract.

      The cosmetic composition for improving odors of the elderly represented by the Changja extract can suppress oxidization of hexadecenoic acid to the general component of the elderly odors, effectively inhibiting the formation of sensation, thereby exhibiting an excellent effect of improving odors.

The cosmetic composition for improving senile odor may be extracted from the lancet, for example, by fractionating and purifying the extract obtained from the lancet to provide a high-purity extract, while simultaneously showing excellent prevention and improvement of senile odor and skin It may not stimulate.

In addition, lemongrass oil can provide a cosmetic composition for preventing and improving geriatric odor by inhibiting normal formation caused by flora.

In one example of the present invention, the added amount of the composition for improving senile odor, which is displayed as a window extract, allows an antioxidant effect and an antibacterial effect to be expressed, but an oxidation inhibitory effect is expressed without impairing the physical properties of the cosmetic composition to be provided. Although it is not particularly limited as long as possible, in one embodiment, the added amount of the composition for improving the elderly odor may be 0.001 to 10.0% by weight relative to the total weight of the cosmetic composition, more preferably 0.01 to 5.0% by weight, even better May be 0.1 to 2.0% by weight, but is not necessarily limited thereto.

In addition, the lemongrass oil extract may be 0.01 to 10.0% by weight relative to the total weight of the cosmetic composition, more preferably 0.05 to 2.0% by weight, but is not necessarily limited thereto.

Cosmetics prepared with a skin cosmetic composition for improving senile odor may include both general emulsifying formulations or solubilizing formulations. Cosmetics in emulsifying formulations include nutrient makeup, cream or essence, and solubilizing formulations in cosmetics include softening makeup. In addition, by containing a skin-scientifically acceptable medium or base, in addition, it can be prepared in the form of auxiliaries that can be applied in the field of dermatology or systemic applications, and cleansing agents that are washable types.

Suitable cosmetic formulations for improving odor, solubilizing formulations can be solutions, gels, solid or dough anhydrous products, and delivery formulations are oil-in-water emulsions, suspensions, microemulsions, microcapsules, microgranules obtained by dispersing the oil phase in an aqueous phase. , It may be a formulation of ionic or nonionic vesicle dispersants (liposomes). The final cosmetic formulation may be provided in the form of a cleansing gel, cleansing foam, cream, skin, lotion, powder, ointment, spray or conceal stick. In addition, it may be prepared in the form of a foam or aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition for improving senile odor according to an embodiment of the present invention is a fat substance, an organic solvent, a solubilizer, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizing agent, a foaming agent, a fragrance, an interface Active agents, water, ionic or nonionic emulsifiers, fillers, metal ion blockers and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, conventional for lipid vesicles or cosmetics It may further contain adjuvants commonly used in cosmetic or dermatological fields, such as any other ingredients used as. In addition, the above ingredients may be introduced in an amount generally used in the field of dermatology.

In addition, as a specific formulation of the cosmetic composition for improving the odor of the elderly according to an embodiment of the present invention, skin lotion, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition Contains formulations such as essences, packs, shampoos, cleansing foams, body lotions and sprays.

On the other hand, as mentioned above, since the extract obtained from Changi-ja may be obtained by fractionation and purification, a method of extracting an ingredient for improving old odor extracted from Changi-ja will be described in more detail.

Method for extracting an active ingredient according to an embodiment of the present invention comprises the steps of: a) extracting the intestine with ethanol to obtain an ethanol extract; And b) sequentially adding and fractionating hexane and ethyl acetate to the ethanol extract to obtain an ethyl acetate fraction containing the fraction.

Through the above extraction method, it is possible to obtain an intestine extract having no skin irritation while simultaneously having an excellent effect of improving elderly odor.

In an example of the present invention, the window of the step a) can be used without limitation, either from the wild or commercially available, but is not limited thereto.

The ethanol of step a) is not particularly limited if it contains ethanol, but using an ethanol having a purity of 70 to 100% by weight, more preferably 75 to 100% by weight, and even more preferably 95 to 100% by weight makes the effective ingredient more effective. It is preferred for extraction.

The amount of ethanol to be added is not particularly limited, but can be extracted by adding 10 times the volume of the intestine.

In addition, in an example of the present invention, step a) can be better extracted at 40 to 100 ° C. for 5 to 48 hours, more preferably at 40 to 60 ° C. for 10 to 20 hours.

In addition, the number of extraction is preferably one to five times repeated extraction, more preferably two to three times repeated extraction, but is not limited thereto. The extraction method may be any of the conventional extraction methods in the art, such as hot water extraction, hot water extraction, ultrasonic extraction, and reflux extraction.

In step b), the extract extracted through step a) is first purified by fractionating it according to the physical properties of the extract. First, ethanol extract is first extracted using hexane to remove non-polar impurities with extremely low polarity. . The number of fractions using hexane is not particularly limited, but is preferably 1 to 5 times, more preferably 2 to 3 times.

Next, extracting with hexane using ethyl acetate and fractionating the remaining filtrate again, from which a fraction containing the extract to be obtained in the present invention can be obtained. This is also not particularly limited, but it is preferable to fractionate 1 to 5 times, more preferably 2 to 3 times.

5 kg of Inula britannica was purchased from Baekje Dangbang, Daejeon-si, and 50 L of purity 95% ethanol was added and reflux was extracted at 79 ± 2 ° C. for 3 hours using an extractor (KSP-248L). The obtained extract was filtered through a filter, and then evaporated ethanol using a reduced pressure concentrator (EYELA N-12) to prepare 270 g of intestinal ethanol extract.

Next, 270 g of Changja ethanol extract was suspended in 8 L of water, hexane (8 L) was added, followed by extraction three times using a separatory filter, and then the remaining aqueous layer was extracted again by adding ethyl acetate (8 L) three times. Water (2L) was additionally added to the remaining aqueous layer, and extracted three times with ethyl acetate (8L). By concentrating the hexane layer and ethyl acetate layer, respectively, 90 g of the hexane fraction and 86 g of the ethyl acetate fraction were obtained.

As in the following examples, it was found through the efficacy activity test that the Changja extract exhibits the elderly odor improvement activity.

DPPH of Changija extract

(1) DPPH radical scavenging activity

      For DPPH radical scavenging activity, 198 μL of 150 μM DPPH solution dissolved in ethanol in a 96-well plate and 2 μL of each sample dissolved in DMSO were added by concentration, and 2 μL of DMSO was added to the control group instead of the sample. After reacting at room temperature for 10 minutes, the absorbance was measured at 517 nm using an ELISA reader to show DPPH radical scavenging activity (%), confirming that it was an extract and completing the present invention.

In order to investigate the DPPH radical inhibitory activity of Changija extract material, free radical scavenging activity was determined as the IC50 value after reacting the sample with the DPPH solution. As a result, as shown in the graph of scavenging activity below and Table 1, it was confirmed that the Changja extract extract inhibited the DPPH radical in a concentration-dependent manner and the IC ₅₀ value was 3.01 mg / ml.

That is, as a result of measuring the DPPH radical scavenging activity by treating each of the Changja extracts 0.781, 1.562, 3.125, and 6.25 mg / ml, when the untreated group of the Changja extract was 100%, 67.85% in the 0.781 mg / ml treatment group, The values of 61.26% in the 1.562 mg / ml treatment group, 46.81% in the 3.125 mg / ml treatment group, and 30.71% in the 6.25 mg / ml treatment group were confirmed to be DPPH radical-depleted in a concentration-dependent manner. It was confirmed from the above results that the IC ₅₀ value was 3.014 mg / ml.

On the other hand, the vitamin C treatment as a control was treated with 3.125, 6,250, 12.5, and 25.0 uM, respectively, to investigate the DPPH radical scavenging activity. As a result, when the untreated extract of Changja extract was 100%, 84.07 in the vitamin C treatment with 3.125 uM It showed DPPH radical scavenging activity of ± 3.30%, 56.04 ± 0.96% in 6.25 uM treatment, 23.35 ± 2.89% in 12.5 uM treatment, and 3.02 ± 1.26% in 25 uM treatment. It was confirmed from the above results that the IC ₅₀ value was 10.189 ug / ml.

[Graph of DPPH radical scavenging activity of window and extract]

[Graph of DPPH radical scavenging activity of Vimin C]

DPPH radical efficacy of Changija extract (IC ₅₀ )    sample             Changija Extract (mg / ml)    density 6.25 3.125 1.562 0.781 0     b 0.1753 0.1957 0.2358 0.3078 0.4359 0.1698 0.2024 0.2583 0.3196 0.4403 0.1791 0.1993 0.2526 0.3208 0.0478     b ' 0.0504 0.0493 0.0492 0.0482 0.0478    b-b ' 0.1249 0.1464 0.2046 0.2596 0.3881 0.1194 0.1531 0.2091 0.2714 0.3925 0.1287 0.1500 0.2034 0.2726 0.3796     % 32.30 37.86 52.90 67.13 100.35 30.87 39.59 54.07 70.18 101.49 33.28 38.79 52.59 70.49 98.16    Average 32.15 38.74 53.19 69.26 100   STDEVS 1.21 0.87 0.78 1.86 1.70    IC50  3014

Antibacterial activity against skin flora

○ Culture conditions

To measure the antiseptic force of the selected sample, a disk diffusion method using the Kirby-Bauer method was used. This method is a general antimicrobial measurement method. It is a method of measuring the size of a transparent area formed after cultivation by placing a number of discs on a solid agar medium coated with a test strain with a known concentration of an antibacterial substance solution. Triclosan was used as a reference substance, and as the strain , the anaerobic properties of Staphylococcus epidermis (KCTC 1917) and Propionibacterium acnes (KCTC 3314) were used. Bacteria was used.

Reinforced Clostridial (RC) medium (Difco. USA) was used as the culture medium for propionibacterium acnes , and freeze-dried Propionibacterium acnes was activated 3 days before the experiment. ) Was used AnaeroGen ^TM 2.5L (Oxoid, UK). The incubation container was sealed and cultured in an anaerobic state for 3 days in a 37 ° C incubator. Anaerobic status inside the container was confirmed using an Anaerobic indicator (Thermo, UK).

As shown in Table 2 below, aerobic strain Staphylococcus epidermis was used Tryptic Soy Agar (Difco. USA), and cultured for 24 hours in an incubator at 37 ° C. after inoculation of the bacteria.

      Strain name      Badge name     Culture conditions Propionibacterium acnes RC 37 ℃ (72 hours) Staphylococcus epidermis        TSA 37 ℃ (24 hours)

○ Preparation of fungus

Dispense 10 ml of dilution into a sterile test tube containing approximately 5 g of glass beads. Take 4 to 5 colonies from each Agar Medium cultured under conditions of 2-2, put them in a 10 ml diluent, and vortex to homogenize them for up to 3 minutes.

At this time, the McFarland turbidity is adjusted to 0.5 so that the number of bacteria is 1 Х10 ⁷ -1 Х10 ⁸ cfu / ml.

 ○ Paper disc test

After the fungus was prepared, Staphylococcus epidermis (KCTC 1917) was prepared by Mueller Hinton Agar (Difco USA) and Propionibacterium acnes (KCTC 3314) using Reinforced Clostridial (RC) medium (Difco. USA).

Thereafter, 0.5 ml of the fungus solution was mixed with 5 ml of each medium cooled to 45 ° C., and then additionally poured into the previously prepared plate medium to prepare the medium for each strain.

Table 3 below puts a paper disc (ø; 8mm, ADVANTEC, Japan) on the medium for each strain, and dilute cypress oil (100%, 50%, 20%) for each concentration using 0.5% HCO60 (Hydrogenated Caster Oil). of 5% HCO60), Lemon glass oil (100%, 50%, 20% of 5% HCO60), Tea tree oil (100%, 50%, 20% of 5% HCO60) Gyejihwang (100%, 50%) , Triclosan (0.3%) was absorbed by 40 µl each. After incubating the plate treated with each sample in an incubator for 24 hours at 37 ° C for Staphylococcus epidermis (KCTC 1917), and Propionibacterium acnes (KCTC 3314) at 37 ° C for 72 hours under anaerobic conditions, clear zones around the filter paper disc Diameter size was measured.

○ The clear zone (mm) was calculated according to the following equation.

W = (T-D2)

W: Clear zone (mm)

T: Overall diameter of sample and stop

D: Sample diameter

STRAINS Media Culture
Temperature Incubation
Time P.acnes (KCTC 3314)  Reinforced Clostridial mdium 37 ℃ 72 hours S.epidermis (KCTC1917) Mueller Hinton medium 37 ℃ 24 hours

The antibacterial activity for each natural substance was diluted by concentration from 100% to 20% of the stock solution. Control was set for each experiment to prevent false positives caused by the antibacterial activity of HCO60 used for dilution of essential oils. It was intended to determine the accuracy of. As a result of the paper diffusion method test for Staphylococcus epidermis (KCTC 1917), the antibacterial activity of each strain using the paper disk method increased as the concentration of the sample increased, and the size of the clear zone increased. First, lemongrass oil (100%, 50%, 20 %), It is judged that the growth of microorganisms on the surface of the medium was found to be suppressed as the color of the medium was changed and the growth of microorganisms was suppressed.

The effect of Changija extract and lemongrass extract on the improvement of body odor

To check the improvement effect of elderly odor, apply the cosmetics of <Formulation Example 1> to 10 women aged 50-70 years 3 times a day for breakfast, lunch, and dinner for 8 weeks and then apply it to the elderly odor area. As a result of investigating the improvement effect of, the improvement effect was clearly shown in Table 4.

Improvement effect of old man's odor division      1 week      4 weeks     8 weeks No treatment      -       -       - Changija extract treatment Female -1      -      ±       + Female -2      -      ±       + Female -3      -      ±       + Female -4      -      ±      + + Female -5      -      ±       + Female -6      -      ±      + + Female -7      -      ±       + Female -8      -      ±       + Female -9      -      ±      + + Female -10      -      ±       +

Reference: No improvement symptoms “-” Slight improvement symptoms “±” Improvement effect “+”

Human body patch test of Changija extract

1. Test method

In the case of this human application test, the stimulus response was evaluated by a 3rd reading process after the 24 hour patch. In the case of a 24-hour patch, two readings are generally performed, but in the case of this test, the intensity of the response is repeatedly and continuously observed to overcome the technical limitations of the single patch test by evaluating the stimulation and sensitization of the Acute & Delayed type. In total, three readings were selected and evaluated. The method used in this test was a single enclosed patch test, and the test was conducted by dividing the patch attachment step that causes the skin irritation response into the visual evaluation step by the expert. As a sample, the Changja extract was used as a sample at a concentration of 0.5% (1,3BG).

end. Patching stage (day 0)

① A special patch (IQ series / IQ Ultra Chamber) was used to evaluate the primary skin irritation patch. IQ Ultra Chamber is a double structure with an inert polyethylene chamber and an adhesive frame on a rectangular non-irritating adhesive tape with a width of 68 mm and a length of 142 mm. It consists of 2 rows per patch, and there are chambers that can load a total of 10 test substances, 5 for each row, and each chamber volume is composed of a maximum of 60 microliter and an area of 9x9mm. The spacing between each chamber is designated as 12 mm between rows and 20 mm between rows.

② As the test sample, 20 ~ 25 microliter, which is sufficient to prevent the loaded sample from flowing out of the patch, was used. In the case of a liquid sample, it was tested after being wetted with a filter provided specially.

③ The patch was attached so that the test sample loaded on the back of the subject spread out evenly on the test site.

I. Visual evaluation stage

① 24 hours after patch installation (1st day)

      -24 hours after the patch was attached, the patch was removed, the test site was wiped clean with a soft cotton cloth moistened with mineral oil, and the test site was marked using a marking pen.

      -After 30 minutes to 1 hour of patch removal, visual evaluation of the test site by two experts was conducted to record the degree of irritation on the evaluation sheet.

② 24 hours after patch removal (Day 2)

      -After 24 hours of patch removal, the test site was visually evaluated by two experts and the degree of skin irritation was recorded on the evaluation sheet.

③ 48 hours after patch removal (Day 3)

     -48 hours after patch removal, the test site was visually evaluated by two experts and the degree of skin irritation was recorded on the evaluation sheet.

2. Visual evaluation method

The evaluation criteria of the stimulus response were applied to the international contact dermatitis research group (ICDRG) reading standards, and were used as the evaluation method devised by Frosch & Kligman. In order to secure the objectivity of visual evaluation, the degree of skin irritation was judged by referring to the picture below, and the representative picture is shown below.

Skin irritation evaluation criteria Stimulation degree Score Types of skin symptoms or signs ±  0.5 Doubtful skin sign such as weak erythema +   One Slight erythema, either spotty or diffuse ++   2 Moderate uniform erythema +++   3 Intense erythema with edema ++++   4 Intense erythema with edema & vesicles -(negative)   - Negative reaction IR (irritant reaction)   - Various other types of stimulation reactions (including adhesion stimulation) NT (not tested)   - Interruption of the test due to the occurrence of an irritation reaction or other reasons

[Representative photo of skin irritation reaction by human patch test]

A   B

C   D

* A: 1 (+) class, B: 2 (++) class, C: 3 (+++) class, D: 4 (++++) class

3. Skin irritation calculation method

The degree of irritation evaluated three times by the criteria was calculated according to the following skin irritation index calculation formula, and the criteria for the irritation index are shown in Table 3. Based on the safety zone according to the product using the average stimulus index and standard deviation (see Table 4.), if the average response index R value with z ≤ 1.00 is less than the value shown in Table 6, the final product is judged to be predictable as a safe product. If it is more than that, it is predicted that the product is highly likely to cause skin irritation when using it.

* Average skin irritation index = (A x 100) / (B x C x D)

A: Sum of observed values at every reading-Σ (Each Score; 0.5, 1, 2, 3, 4)

       B: Maximum skin irritation evaluation score used in the test 4

       C: Total number of subjects participating in the test

       D: Total reading order 3

Skin irritation level Average skin irritation index Judgment rating 0.00 ~ 0.86 Slight grade 0.87 ~ 2.43 Mild grade 2.42-3.43 Moderate grade 3.44 ~ Severe grade

4. Prediction of the possibility of primary stimulation due to product release

In cosmetics, irritation is not only caused by the interaction between the contents of the product itself and the skin, but there is a possibility that skin irritation may be caused by various factors depending on the behavior of the actual product, the user's race, climate, and the skin condition of the subject. Therefore, the safety test is called a prediction test, and the safety cannot be overstated.

In the case of the primary stimulation patch test currently being performed due to skin irritation caused by cosmetics caused by these various factors, there is no standard for judging whether a product is commonly used worldwide. However, according to the recent research results, it is considered that it is safe to set a safety zone considering the actual use of the product and use it as a criterion for stimulation.

In addition, in a test involving 50 or fewer subjects, a test product in which a reaction occurs at a frequency exceeding 20% of the total subjects when reading a single patch test or a stimulus response of +2 or more upon each reading In the case of test products observed at 10% or more, it can be predicted that the test products are highly likely to cause irritation.

Therefore, in the case of this test, the safety zone (Table 7), the stimulus-producing product with a frequency exceeding 20% of the subjects, the test product with a stimulus response of +2 or more observed in each reading, was observed in 10% or more of the total subjects. If applicable, it was judged as a test product with a potential to cause irritation.

Safety zone considering actual product by human body primary stimulation test Product category Safety zone z ≤ 1.00 mean
Reaction index R value Leave
-on Toners
Lotions and creams
Foundations
Sunscreen creams
Lip products
Body lotions and creams
Face powders, eye shadows
Eye liners, mascaras 0.87
1.12
0.97
0.83
0.98
0.94
1.02
2.96 Tissue
-off Make-up removers
Packs and massage creams 1.36
1.33 Wash
-off Facial cleansers
Soap and bath
Shampoos, rinses and hair treatments 3.20
3.98
2.82

○ Products that cause irritation with a frequency of more than 20% of subjects: 30 x 0.2 = 6.0 Therefore, in this test, products that caused irritation from 6 or more subjects

    ○ A test product with a stimulus response of +2 or more observed during each reading in 10% or more of the total subjects

5. Results

○ Average skin irritation index by test product

After evaluation according to the evaluation criteria, the average irritation index was calculated according to the calculation formula and the skin irritation intensity was evaluated according to the evaluation criteria. By determining the final skin irritation in accordance with three criteria: safety zone according to the product, irritation-inducing products with a frequency exceeding 20% of subjects, and test products with a stimulus response of +2 or more observed at every reading. Table 8 shows the final prediction of the suitability of the product.

Skin primary irritation readings Sample name Sum of skin irritation response intensity Skin irritation
Indices skin
Irritation
Judgment
Rating Safety
zone Subject
20%
Excess +2
More than
10%
More than Suitability
Judgment Other Primary Secondary 3 car Changija Extract 0.00 0.00 0.00 0.00 Slight fitness fitness fitness fitness DW
(Voice control) 0.00 0.00 0.00 0.00 Slight fitness fitness fitness fitness 1% SLS
(Positive control) 0.00 2.00 1.50 0.97 Mild incongruity fitness fitness fitness

Meanwhile, the extracts of the present invention can be formulated in various forms according to the purpose. Illustrative formulation examples for the compositions of the invention.

(Formulation Example 1)

Preparation of convergent lotion

Butyl glycol, glycerin, betaine, citric acid, and sodium citrate were added to the purified water, followed by dissolution, followed by dissolution of a mixture of perfume, polyoxyethylene (60) cured castor oil in ethanol, and slowly added. To this, the Changji extract of the present invention and lemongrass oil (manufacturer: using SRI VENKATESH AROMAS) were added and stirred sufficiently and aged in a storage room. Table 9 shows the content of each component.

   Raw material     Content (w / w%) Changija Extract 0.5 Lemongrass oil 0.1 Butylene glycol 5.0 glycerin 1.0 Polyoxyethylene (60) hardened castor oil 0.5 ethanol 4.0 Betaine 1.0 Beta glucan 0.5 Niacinamide 2.0 Citric acid 0.02 Sodium citrate 0.06 Preservative a very small amount Spices a very small amount Purified water to: 100

(Formulation Example 2)

Preparation of body lotion

In the present invention, the butylene glycol, glycerin, carboxyvinyl polymer, arginine, allantoin, preservative and purified water were heated while stirring to 70 to 75 ° C. Squalane, butylene glycol dicaprylate / dicaprate, sorbitan stearate, polysorbate 60, glyceryl stearate, rice bran oil, cetyl alcohol, and stearyl glycol heated with stirring at 75 to 80 ° C After adding the nate mixture and emulsifying it with a homogenizer, the mixture was added by mixing Changi extract, lemongrass oil, and spices while cooling to about 45 ° C while stirring, and then cooled to 30 ° C and aged to produce nutrient makeup. Table 10 shows the content of each component.

             Raw material    Content (w / w%) Changija Extract 1.0 Lemongrass oil 0.2 Butylene glycol 5.0 glycerin 5.0 Squalane 8.0 Rice bran oil 2.0 Butylene glycol dicaprylate / dicaprate 5.0 Sorbitan stearate 1.5 Polysorbate 60 1.5 Glyceryl stearate S 0.5 Cetyl alcohol 2.0 Stearyl glycyrrhinate 0.2 Allantoin 0.1 Sodium hyaluronate 0.2 Niacinamide 2.0 Carboxyvinyl polymer 0.1 Arginine 0.1 Preservative a very small amount Spices a very small amount Purified water to: 100

(Formulation Example 3)

Preparation of essence

Phytosterol, polyglyceryl 2-oleate, disetyl phosphate, ceramide, ceteareth-4, and cholesterol were dissolved by stirring to 70-80 ° C, followed by stirring purified water, allantoin, glycerin, xanthan gum, beta-glucan, etc. to 70 ° C After emulsifying and cooling, the mixture of the present invention was added at 45 ° C., and fragrance was added to the mixture, followed by cooling to 30 ° C., followed by aging. Table 11 shows the content of each component.

           Raw material Content (w / w%) Changija Extract 1.0 Lemongrass oil 0.1 Allantoin 0.1 Polyglyceryl 2-oleate 1.5 Ceramide 0.7 Seteares-4 1.2 S-oligopeptide-1 0.00001 S-Polypeptide-11 0.00001 S-polypeptide-1 0.00001 S-Polypeptide-22 0.00001 cholesterol 1.5 Dicetyl phosphate 0.4 1,3BG 5.0 Beta glucan 0.5 Concentrated glycerin 0.5 Carboxyvinyl polymer 0.2 Shotgun 0.2 antiseptic a very small amount Spices a very small amount Purified water to: 100

(Formulation Example 4)

Cream for the elderly

Changgija extract of the present invention, lemongrass oil, butylene glycol, glycerin, beta glucan, betaine, white fungus extract, polyglutamic acid, carboxyvinyl polymer, arginine, allantoin, preservatives and purified water to 70 to 75 ℃ Heated while stirring. To this, a mixture of squalane, sunflower seed oil, jojoba oil, sorbitan stearate, polysorbate 60, cetostearyl alcohol, beads wax, glyceryl stearate, and cocoa butter heated by stirring at 75 to 80 ° C was added to homogena. After emulsifying with ezer, while stirring and cooling to about 45 ° C, Pycnogenol, Niacinamide, Ascorbyl glucoside, and fragrance were added and mixed, followed by cooling to 30 ° C. Table 12 shows the content of each component.

Raw material Content (w / w%) Changija Extract 1.0 Lemongrass oil 0.2 Butylene glycol 8.0 glycerin 5.0 Squalane 10.0 Grape seed oil 3.0 Butylene glycol dicaprylate / dicaprate 5.0 Sorbitan stearate 1.5 Polysorbate 60 1.0 Glyceryl stearate 0.5 Stearyl glycinate 0.2 Cocoa butter 2.0 Beads wax 1.0 Cetostearyl alcohol 3.0 Ascorbyl glucoside 0.5 Allantoin 0.1 Sodium hyaluronate 0.2 Niacinamide 2.0 Carboxyvinyl polymer 0.1 Arginine 0.1 Preservative a very small amount Spices a very small amount Purified water to: 100

(Formulation Example 5)

Aged cleanser

Changgija extract, lemongrass oil, butylene glycol, glycerin, myristic acid, lauric acid, allantoin, preservative and purified water of the present invention were heated with stirring to 70 to 75 ° C. Here, KOH was added and emulsified with a homogenizer, followed by mixing while adding ubiquinone and fragrance while cooling to about 45 ° C while stirring, and then cooling to 30 ° C to prepare.

          Raw material     Content (w / w%) Changija Extract 1.0 Lemongrass oil 0.2 Disodium cocoamphodiacetate 10 Lauric acid 8.0 Myristic Acid 5.0 Potassium hydroxide 10.0 Lauraamide D 3.0 Ubiquinone 0.1 Monterey Pine Bark Extract 1.5 Spices 1.0 Allantoin 0.5 Phenoxyethanol 0.2 Dipotassium glyceride 2.0 Disodium LED 1.0 1,2 hexanediol 1.5 Purified water to: 100

Although the preferred embodiments of the present invention have been described above, it is clear that the present invention can use various changes, modifications, and equivalents, and can be equally applied by appropriately modifying the above embodiments. Therefore, the above description is not intended to limit the scope of the present invention as defined by the following claims.

Claims (8)

Cosmetic composition for improving elderly odor using natural ingredients containing Changija extract as an active ingredient.
According to claim 1,
A cosmetic composition for improving senile odor using a natural product containing lemongrass oil extract as an active ingredient in the Changja extract.
According to claim 1,
Changija extract is a cosmetic composition for improving elderly odor using natural products containing 0.001 to 10.0% by weight based on the total weight of the cosmetic composition.
According to claim 2,
A cosmetic composition for improving elderly odor using natural products containing 0.01 to 10.0% by weight of Changja extract and 0.01 to 0.001 to 10.0% by weight of lemongrass oil extract with respect to the total weight of the cosmetic composition.
According to any one of claims 1 and 2,
The Changja extract,
a) extracting an intestine with ethanol to obtain an ethanol extract; And
b) a step of sequentially adding and fractionating hexane and ethyl acetate to the ethanol extract to obtain an extract; a cosmetic composition for improving elderly odor using a natural product comprising a.
The method of claim 5,
The a) step is a cosmetic composition for improving elderly odor using natural products performed for 5 to 48 hours at 40 to 100 ° C.
The method of claim 5,
The b) step is a cosmetic composition for improving senile odor using a natural product comprising the step of obtaining the acetic acid fraction from 1 to 5 fractions.
According to any one of claims 1 and 2,
The cosmetic composition;
Nutrient makeup, softening lotion, cream, essence, cleansing foam, cleansing gel, lotion, powder, ointment, spray, conceal stick, solution, gel, solid, suspension, microemulsion, micro capsule, microgranules, ionic vesicle dispersant, non A cosmetic composition for improving elderly odor using natural products formulated with at least one selected from the group consisting of ionic vesicle dispersants.