KR20190133660A - 변형된 피코르나바이러스 3c 프로테아제 및 그의 방법 - Google Patents

변형된 피코르나바이러스 3c 프로테아제 및 그의 방법 Download PDF

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KR20190133660A
KR20190133660A KR1020197004446A KR20197004446A KR20190133660A KR 20190133660 A KR20190133660 A KR 20190133660A KR 1020197004446 A KR1020197004446 A KR 1020197004446A KR 20197004446 A KR20197004446 A KR 20197004446A KR 20190133660 A KR20190133660 A KR 20190133660A
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마이클 푸케트
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더 가버먼트 오브 더 유나이티드 스테이츠 오브 아메리카, 에즈 레프리젠티드 바이 더 세크러테리 오브 홈랜드 시큐리티
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Abstract

본원은 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것으로서, 상기 변형된 피코르나바이러스 3C 프로테아제는 변경된 2차 구조 및 야생형 구제역 바이러스 (FMDV) 3C 프로테아제의 위치 16 내지 25, 99, 100 및 115 내지 130에 상응하는 하나 이상의 아미노산 위치(들)에 위치되는 하나 이상의 아미노산 치환(들)을 포함하고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 상기 단리된 폴리뉴클레오타이드는, 숙주 세포에서 형질전환되고 공동-발현될 때, 대조군 피코르나바이러스 3C 프로테아제에서 형질전환되고 공동-발현된 숙주 세포에서 나타내는 P1 전구체 폴리펩타이드 형질전환 유전자의 발현양과 비교하여, P1 전구체 폴리펩타이드의 형질전환 유전자 발현을 증가시키고, 상기 하나 이상의 상응하는 아미노산 위치(들)은 상기 변형된 피코르나바이러스 3C 프로테아제와 하나 이상의 야생형 FMDV 3C 프로테아제(들)의 정렬에 의해 동정된다. 상기 단리된 폴리뉴클레오타이드를 사용하여 피코르나바이러스 P1 전구체 폴리펩타이드를 피코르나바이러스 바이러스 단백질 및/또는 바이러스-유사 입자로 가공하는 방법이 또한 제공된다.

Description

변형된 피코르나바이러스 3C 프로테아제 및 그의 방법
[연관 출원에 대한 상호 참조]
본원은, 그 전문이 본원에 참조로서 포함되고, 2018 년 5 월 22 일자로 출원된 미국 출원 제 15/986,214 호의 일부 계속 출원이다.
[서열 목록]
본원은 EFS-Web을 통해 제출된 서열 목록을 포함하고, 그 전문이 본원에 참조로서 포함된다. 2018 년 5 월 20 일에 작성된 ASCII 사본은 DHS-0162_ST.25.txt로 명명되고, 크기는 1,892 KB이다.
[분야]
본원은 변형된 피코르나바이러스 3C 프로테아제(picornaviruses 3C proteases)에 관한 것이다. 상기 변형된 피코르나바이러스 프로테아제는 숙주 세포에서 발현될 때 감소된 세포 독성을 나타낸다.
아프토바이러스(Aphthovirus) [예: 구제역 바이러스 (Foot-and-Mouth Disease Virus, FMDV)], 엔테로바이러스 (Enterovirus) (예: 폴리오바이러스 및 리노바이러스) 및 헤파토바이러스 (Hepatovirus) [A형 간염 바이러스 (Hepatitis A virus, HAV)]와 같은 속(genera)을 포함하는 피코르나바이러스는 인간과 동물의 건강에 중요한 영향을 미친다. 일부 감염은 경미할 수 있지만, 많은 피코르나바이러스 균주는 심각한 질병과 실질적인 경제적 부담을 유발한다.
구조적으로, 피코르나바이러스는 단일-가닥의 게놈 RNA의 코어를 둘러싸고 있는 캡시드 (capsid)를 특징으로 하는 비외피형 (non-enveloped) RNA 바이러스이다. 상기 게놈 RNA는 하나의 개방 판독 프레임(open reading frame)을 포함하고, 이것은 바이러스-코딩된 프로테아제에 의해 구조 및 비구조 단백질들로 순차적으로 절단되는 큰 폴리프로테인으로 번역된다. 상기 캡시드를 조립하는 데 사용되는 상기 구조 단백질은 궁극적으로 중간 절단 생성물, P1 (또한 P1-2A라고도 알려짐)에서 유도된다.
상기 조립된 캡시드 (바이러스-유사 입자: Virus-Like Particles 또는 VLPs)만을 사용하는 다양한 피코르나바이러스 균주에 대한 서브유닛(subunit) 백신이 현재 개발 중에 있다. 숙주 세포에서 재조합적으로 발현될 수 있는, 이러한 다중단백질 구조는 진정(authentic) 천연 바이러스의 구조 및 형태를 모방하지만, 상기 바이러스 게놈이 결여되어 있다. 결과적으로, 바이러스의 일부만을 포함하는 그러한 백신은, 예를 들어, 보다 일반적으로 사용되는 비활성화된 전바이러스 생 백신(whole live viral vaccines) 보다 제조 과정 동안 우연한 발병의 결과를 일으킬 가능성이 적다.
VLPs를 포함하는 피코르나바이러스 서브유닛 백신의 잠재적 안전성에도 불구하고, 상기 구조를 숙주 세포로부터 수득하는 것이 어려울 수 있다. 예를 들어, 피코르나바이러스 3C 프로테아제 (또는 3Cpro), 또는 3Cpro와 3D 폴리머아라제 (또는 3CD)의 융합 단백질의 세포질 발현은 P1 폴리프로테인을 상기 캡시드로 조립되는 개별적인 구조 단백질로 가공하기 위해 전형적으로 요구된다. 그러나, 숙주 세포에서 3Cpro 또는 3CD의 사용은 세포 생존과 연관된 것들을 포함하여, 다양한 숙주 단백질의 단백질 분해 (proteolysis)를 일으킬 수 있다. 따라서, 숙주 세포에서 야생형 3Cpro 또는 3CD의 발현은 백신 제조 플랫폼 옵션을 제한하고 항원 수율을 감소시킬 수 있다.
최근 보고서는, 재조합 FMDV P1 폴리프로테인을 가공하는 능력을 유지하면서, 대장균(E.coli)과 같은 숙주 세포에 대한 해로운 영향을 완화시키는 FMDV 3C 프로테아제 돌연변이체를 기술하고 있다. 그러나, 상기 특성들을 가진 다른 피코르나바이러스 3C 또는 3CD 프로테아제 돌연변이체는 기술되어 있지 않다. 또한, 신규 FMDV 3C 프로테아제 돌연변이체가 대장균, 포유동물 숙주 세포 또는 다른 발현 시스템에서 추가적인 이점을 제공할 수 있기 때문에 바람직하다. 결과적으로, 숙주 세포에서 피코르나바이러스 3C 프로테아제의 상기 발현을 촉진시키는 추가 피코르나바이러스 3C 또는 3CD 프로테아제 돌연변이체에 대한 요구가 있다.
제 1 측면에 있어서, 본원은 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것으로서, 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 구제역 바이러스[Foot-and-Mouth Disease Virus (FMDV)] 3C 프로테아제의 위치 16 내지 25, 99, 100, 및 115 내지 130에 상응하는 하나 이상의 아미노산 위치(들)에 위치된 하나 이상의 아미노산 치환(들)을 포함한다. 전형적으로, 본원에 기재된 상기 야생형 FMDV 3C 프로테아제는 서열 번호 2, 4, 6, 8, 10, 12, 14, 16, 18 및 20 중에서 선택된다.
일부 구현예들에 있어서, 본원에 정의된 것과 같이, 상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 상기 단리된 폴리뉴클레오타이드는, 숙주 세포에서 형질전환되고 공동-발현될때, 대조군 피코르나바이러스 3C 프로테아제로 형질전환되고 공동-발현되는 숙주 세포에서 나타나는 P1 전구체 폴리펩타이드 형질전환 유전자(transgene) 발현양과 비교하여, P1 전구체 폴리펩타이드의 형질전환 유전자 발현을 증가시킨다.
일부 구현예들에 있어서, 본원에 기재된 것과 같이, 변형된 FMDV 3C 프로테아제와 같은 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 피코르나바이러스 P1 전구체 폴리펩타이드에 대한 단백질 분해 활성을 나타낸다.
일부 구현예들에 있어서, 본원에 기재된 것과 같이, 변형된 FMDV 3C 프로테아제와 같은 본원의 단리된 폴리뉴클레오타이드에 의해 코딩되는 상기 변형된 피코르나바이러스 3C 프로테아제는, 대조군 피코르나바이러스 3C 프로테아제를 발현하는 숙주 세포에서 단백질 분해에 의해 절단된 eIF4A1와 비교하여, 숙주 세포에서 단백질 분해에 의해 절단된 eIF4A1의 양의 증가 또는 감소를 나타낸다. 전형적으로, 상기 변형된 피코르나바이러스 3C 프로테아제는, 대조군 피코르나바이러스 3C 프로테아제를 발현하는 숙주 세포에서 단백질 분해에 의해 절단된 eIF4A1의 양과 비교하여, 숙주 세포에서 단백질 분해에 의해 절단된 eIF4A1 양의 감소를 나타낸다.
일부 구현예들에 있어서, 본원에 기재된 것과 같이, 변형된 FMDV 3C 프로테아제와 같은 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는, 대조군 피코르나바이러스 3C 프로테아제를 발현하는 숙주 세포에서 히스톤 H3, NEMO 또는 SAM68의 양과 비교하여, 히스톤 H3, 핵 전사 인자 카파 B 필수 조절 인자 (nuclear transcription factor kappa B essential modulator, NEMO), 또는 유사분열에서 68 kDa의 Src-연관 기질 (Src-associated substrate, SAM68)의 양의 증가 또는 감소를 나타낸다. 전형적으로, 상기 변형된 피코르나바이러스 3C 프로테아제는, 대조군 피코르나바이러스 3C 프로테아제를 발현하는 숙주 세포에서 히스톤 H3, NEMO, 또는 SAM68의 양과 비교하여, 숙주 세포에서 히스톤 H3, NEMO, 또는 SAM68의 양의 감소를 나타낸다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 상기 단리된 폴리뉴클레오타이드는 상기 변경된 단백질 2차 구조를 발현한다. 예를 들어, 변경된 알파-헬릭스, 변경된 베타 시트, 변경된 루프 등, 전형적으로, 상기 변경된 2차 구조는 변경된 A1-B1 β 시트, F1 β 가닥과 A2 β 가닥 사이의 변경된 루프, 및 변경된 A2-B2 시트로부터 선택되고, 상기 하나 이상의 아미노산 치환(들)이 하기 중에서 적어도 하나로부터 선택되는 하나 이상의 위치에 위치된다: 야생형 구제역 바이러스(FMDV) 3C 프로테아제의 상기 A1-B1 β 시트에서 아미노산 16-25 중에서 하나 이상에 상응하는 위치(들), 야생형 FMDV 3C 프로테아제의 F1 β 가닥과 A2 β 가닥 사이의 상기 루프에서 아미노산 99 및 100 중에서 하나 이상에 상응하는 위치(들), 및 야생형 FMDV 3C 프로테아제의 상기 A2-B2 β 시트에서 아미노산 115-130 중에서 하나 이상에 상응하는 위치(들).
일부 구현예들에 있어서, 아미노산 위치에 상응하는 상기 하나 이상은 상기 야생형 FMDV 3C 프로테아제의 상기 하나 이상의 1차 아미노산 서열과 상기 변형된 피코르나바이러스 3C 프로테아제의 1차 아미노산 서열의 정렬(alignment)에 의해 동정된다. 전형적으로, 상기 정렬은 기본(default) 매개변수를 갖는 Blossum 62을 이용하여 2015년 1월 1일 Clone Manager 9, Professional Edition, Version 9.4을 사용하여 수행된다.
일부 구현예들에 있어서, 아미노산 위치에 상응하는 상기 하나 이상은 본원에서 기재된 것과 같이 상기 야생형 FMDV 3C 프로테아제의 상기 하나 이상의 단백질 형태(conformation)와 상기 변형된 피코르나바이러스 3C 프로테아제의 단백질 형태를 비교함으로써 동정된다. 그러나, 전형적으로 상기 1차 아미노산 서열들의 정렬은 상응하는 아미노산 위치를 결정하기 위해 사용된다.
일부 구현예들에 있어서, 본원의 상기 변형된 피코르나바이러스 3C 프로테아제는 변형된 FMDV 3C 프로테아제를 배제한다. 다른 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 하나 이상의 아미노산 치환들을 갖는 변형된 FMDV 3C 프로테아제를 배제하고, 상기 하나 이상의 아미노산 치환들은 위치 125에서의 아미노산 치환, 위치 126에서의 아미노산 치환, 위치 127에서의 아미노산 치환, 위치 128에서의 아미노산 치환, 위치 129에서의 아미노산 치환, 위치 130에서의 아미노산 치환 및 이들의 조합들로 이루어진 군으로부터 선택된다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 아프토바이러스 (Aphthovirus), 아쿠아마바이러스 (Aquamavirus), 아비헤파토바이러스 (Avihepatovirus), 카디오바이러스 (Cardiovirus), 코사바이러스 (Cosavirus), 디시피바이러스 (Dicipivirus), 엔테로바이러스 (Enterovirus), 에르보바이러스(Erbovirus), 헤파토바이러스 (Hepatovirus), 코부바이러스 ( Kobuvirus ), 메기바이러스 (Megrivirus), 파레코바이러스 (Parechovirus), 피스세바이러스 (Piscevirus), 살리바이러스 (Salivirus), 사펠로바이러스 (Sapelovirus), 세네카바이러스(Senecavirus), 테스코바이러스 (Teschovirus) 및 트레모바이러스(Tremovirus)를 포함하는 상기 피코르나바이러스 속의 임의의 것으로부터의 임의의 종, 아형(subtype) 및/또는 혈청형(serotype)으로부터 수득될 수 있고 또는 유도될 수 있다. 전형적으로, 상기 속은 아프토바이러스 (Aphthovirus), 엔테로바이러스 (Enterovirus) 및 헤파토바이러스 (Hepatovirus)를 포함한다.
상기 아프토바이러스 (Aphthovirus) 속 내에 전형적인 종들은 소 비염 A 바이러스 (Bovine rhinitis A virus), 소 비염 B 바이러스 (Bovine rhinitis B virus), 말 비염 A 바이러스(Equine rhinitis A virus) 및 구제역 바이러스 (Foot-and-mouth disease virus, FMDV)를 포함한다. 전형적인 엔테로바이러스 (Enterovirus) 종들은 엔테로바이러스 A (Enterovirus A), 엔테로바이러스 B (Enterovirus B), 엔테로바이러스 C (Enterovirus C), 리노바이러스 A (Rhinovirus A) 및 리노바이러스 B (Rhinovirus B)를 포함한다. 상기 헤파토바이러스 (Hepatovirus) 내에 전형적인 종은 A형 간염 바이러스 (Hepatitis A virus)를 포함한다.
FMDV 내에 전형적인 바람직한 혈청형은 O, A, C, Southern African Territories (SAT) 1, SAT 2, SAT 3 및 Asia 1을 포함한다.
엔테로바이러스 A (Enterovirus A) 내에 전형적인 바람직한 혈청형은 콕사키바이러스 A10 (coxsackievirus A10) 및 엔테로바이러스 A71 (Enterovirus A71)을 포함한다.
엔테로바이러스 B (Enterovirus B) 내에 전형적인 바람직한 혈청형은 돼지 소낭병 바이러스 (swine vesicular disease virus), 콕사키바이러스 B1 (coxsackievirus B1), 및 콕사키바이러스 B3 (coxsackievirus B3), 더 전형적으로 콕사키바이러스 B1 (coxsackievirus B1) 및 콕사키바이러스 B3 (coxsackievirus B3)를 포함한다.
엔테로바이러스 C (Enterovirus C) 내에 전형적인 바람직한 혈청형은 콕사키바이러스 A1 (coxsackievirus A1), 폴리오바이러스 1, 폴리오바이러스 2 및 폴리오바이러스 3을 포함한다.
리노바이러스 A (Rhinovirus A) 내에 전형적인 바람직한 혈청형은 인간 리노바이러스 A2 (Human rhinovirus A2), 인간 리노바이러스 A20 및 인간 리노바이러스 A89를 포함한다.
리노바이러스 B (Rhinovirus B) 내에 전형적인 바람직한 혈청형은 인간 리노바이러스 B14를 포함한다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 하기로부터 선택된다: 변형된 FMDV 3C 프로테아제, 변형된 소 비염 A 바이러스 (Bovine rhinitis A virus) 3C 프로테아제, 변형된 소 비염 B (Bovine rhinitis B) 3C 프로테아제, 변형된 말 비염(Equine rhinitis) 3C 프로테아제와 같은 변형된 아프토바이러스 (Aphthovirus) 3C 프로테아제; 변형된 엔테로바이러스 A (Enterovirus A) 3C 프로테아제, 변형된 엔테로바이러스 B (Enterovirus B) 3C 프로테아제, 변형된 엔테로바이러스 C (Enterovirus C) 3C 프로테아제, 변형된 리노바이러스 A (Rhinovirus A) 3C 프로테아제, 변형된 리노바이러스 B (Rhinovirus B) 3C 프로테아제와 같은 변형된 엔테로바이러스 (Enterovirus) 3C 프로테아제; 및 변형된 A 형 간염 바이러스(Hepatitis A virus) 3C 프로테아제와 같은 변형된 헤파토바이러스 (Hepatovirus) 3C 프로테아제.
일부 구현예들에 있어서, 상기 하나 이상의 아미노산 치환들은 임의의 아미노산 치환(들)을 포함한다. 전형적으로, 상기 아미노산 치환(들)은 비-보존적 치환을 포함한다. 더 전형적으로, 상기 아미노산 치환은 프롤린이다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는, 야생형 FMDV 3C 프로테아제의 잔기 16-25, 99-100 및 115-124와 같은, 잔기 16-25, 99-100 및 115-130 내에 또는 이에 상응하는 것에 하나 이상의 아미노산 치환을 갖는 변형된 FMDV 3C 프로테아제; 야생형 소 비염 A (Bovine rhinitis A) 3C 프로테아제의 잔기 16-25, 95, 96 및 111-124 내에 또는 이에 상응하는 것에 하나 이상의 아미노산 치환들을 갖는 변형된 소 비염(Bovine rhinitis) 3C 프로테아제; 야생형 소 비염 B (Bovine rhinitis B) 3C 프로테아제의 잔기 16-25, 96, 97 및 112-125 내에 또는 이에 상응하는 것에 하나 이상의 아미노산 치환들을 갖는 변형된 소 비염 3C 프로테아제; 야생형 말 비염 A (Equine rhinitis A) 3C 프로테아제의 잔기 16-25, 99-100 및 115-130 내에 또는 이에 상응하는 것에 하나 이상의 아미노산 치환들을 갖는 변형된 말 비염 A (Equine rhinitis A) 3C 프로테아제; 야생형 인간 콕사키바이러스(Human coxsackievirus) A10 3C 프로테아제의 잔기 19-24, 86, 87 및 100-113 내에 또는 이에 상응하는 것에 하나 이상의 아미노산 치환들을 갖는 변형된 엔테로바이러스 A (Enterovirus A) 3C 프로테아제; 야생형 인간 엔테로바이러스 A71 3C 프로테아제의 잔기 19-24, 86, 87 및 100-113 내에 또는 이에 상응하는 것에 하나 이상의 아미노산 치환들을 갖는 변형된 엔테로바이러스 A (Enterovirus A) 3C 프로테아제; 야생형 인간 콕사키바이러스(Human coxsackievirus) B1 또는 B3 3C 프로테아제의 잔기 18-23, 87, 88 및 102-112 내에 또는 이에 상응하는 것에 하나 이상의 아미노산 치환들을 갖는 변형된 엔테로바이러스 (Enterovirus) B 3C 프로테아제; 야생형 인간 콕사키바이러스(Human coxsackievirus) A1 3C 프로테아제의 잔기 19-24, 86, 87 및 100-113 내에 또는 이에 상응하는 것에 하나 이상의 아미노산 치환들을 갖는 변형된 엔테로바이러스 C (Enterovirus C) 3C 프로테아제; 야생형 폴리오바이러스 3C 프로테아제의 잔기 18-23, 86, 87 및 100-113 내에 또는 이에 상응하는 것에 변형된 엔테로바이러스 C (Enterovirus C) 3C 프로테아제; 야생형 리노바이러스 A (Rhinovirus A) 또는 야생형 리노바이러스 B (Rhinovirus B)의 잔기 18-23, 86, 87 및 101-114 내에 또는 이에 상응하는 것에 하나 이상의 아미노산 치환들을 갖는 변형된 리노바이러스 3C 프로테아제; 야생형 인간 A형 간염 바이러스 (Hepatitis A virus) 3C 프로테아제의 잔기 15-20, 99, 100 및 124-131 내에 또는 이에 상응하는 것에 하나 이상의 아미노산 치환들을 갖는 헤파토바이러스 (Hepatovirus) 3C 프로테아제로부터 선택된다.
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는, 야생형 FMDV 3C 프로테아제의 I22 및 L23 중에서 선택되는 것과 같은, I99, L99 및 T100 중에서 선택되는 것과 같은, 또는 D123, V124, G125 및 I128 중에서 선택되는 것과 같은, I22, L23, I99, L99, T100, D123, V124, G125, R126 및 I128 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 갖는 하나 이상의 변형된 FMDV 3C 프로테아제; 야생형 소 비염 A (Bovine rhinitis A) 바이러스 3C 프로테아제의 V22 및 C23 중에서 선택되는 것과 같은, 또는 T96와 같은, 또는 L123 및 F124 중에서 선택되는 것과 같은, V22, C23, T96, L123 및 F124 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 소 비염 A (Bovine rhinitis A) 바이러스 3C 프로테아제; 야생형 소 비염 B (Bovine rhinitis B) 3C 프로테아제의 V22, 및 R23 중에서 선택되는 것과 같은, 또는 T97과 같은, 또는 V121 및 L124 중에서 선택되는 것과 같은, V22, R23, T97, V121 및 L124 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 소 비염 B (Bovine rhinitis B) 바이러스; 야생형 말 비염 A (Equine rhinitis A) 3C 프로테아제의 T22 및 C23 중에서 선택되는 것과 같은, 또는 T100과 같은, 또는 A125 및 T127 중에서 선택되는 것과 같은, Y22, C23, T100, A125 및 T127 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 말 비염 A (Equine rhinitis A) 3C 프로테아제 중 하나 이상에서 선택되는 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제일 수 있다.
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는, 하기 중 하나 이상과 같은, 하나 이상의 변형된 엔테로바이러스 A (Enterovirus A) 3C 프로테아제 중에서 선택되는 변형된 엔테로바이러스(Enterovirus) 3C 프로테아제이다: 야생형 인간 콕사키바이러스(Human coxsackievirus) A10 바이러스 3C 프로테아제의 A71 3C의 Q19 및 T20 중에서 선택되는 것과 같은, 또는 T87과 같은, 또는 L102, I104, N105, M109 및 M112 중에서 선택되는 것과 같은, Q19, T20, T87, L102, I104, N105, M109 및 M112 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 하나 이상의 변형된 인간 콕사키바이러스(Human coxsackievirus) A10 바이러스, 및 야생형 인간 엔테로바이러스 A71 3C 프로테아제의 Q19, 및 Q20 중에서 선택되는 것과 같은, 또는 T87과 같은, 또는 I104, N105, T106 및 M109 중에서 선택되는 것과 같은, Q19, T20, T87, I104, N105, T106 및 M109 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 인간 엔테로바이러스 A71 3C 프로테아제; 야생형 인간 콕사키바이러스 B1의 K19 및 T20 중에서 선택되는 것과 같은, 또는 R87과 같은, 또는 L102, I104, N105, T106, F109, M112 및 I114 중에서 선택되는 것과 같은, K19, T20, R87, L102, I104, N105, T106, F109, M112 및 I114 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 인간 콕사키바이러스 B1 3C 프로테아제와 같은, 변형된 엔테로바이러스 B (Enterovirus B) 3C 프로테아제, 및 야생형 인간 콕사키바이러스 B3 3C 프로테아제의 K19 및 T20 중에서 선택되는 것과 같은, 또는 R87과 같은, 또는 L102, I104, N105, T106, F109, M112 및 I114 중에서 선택되는 것과 같은, K19, T20, R87, L102, I104, N105, T106, F109, M112 및 I114 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 인간 콕사키바이러스 B3 3C 프로테아제; 야생형 콕사키바이러스 A1 3C 프로테아제의 T19 및 T20 중에서 선택되는 것과 같은, 또는 R87과 같은, 또는 L102, V104, N105, T106, F109 및 M112 중에서 선택되는 것과 같은, T19, T20, R87, L102, V104, N105, T106, F109 및 M112 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 하나 이상의 변형된 콕사키바이러스 A1 3C 프로테아제 중에서 선택된 변형된 엔테로바이러스 C (Enterovirus C) 3C 프로테아제, 및 야생형 인간 폴리오바이러스 3C 프로테아제의 T19 및 T20 중에서 선택되는 것과 같은, 또는 R87과 같은, 또는 L102, I103, 및 V104 중에서 선택되는 것과 같은, T19, T20, R87, L102, I103, 및 V104 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 인간 폴리오바이러스 3C 프로테아제; 야생형 리노바이러스 A (Rhinovirus A) 3C 프로테아제의 T19 및 T20 중에서 선택되는 것과 같은, 또는 I86 및 R87 중에서 선택되는 것과 같은, 또는 L102, L104, Q108, T112 및 I114 중에서 선택되는 것과 같은, T19, T20, I86, R87, L102, L104, Q108, T112 및 I114 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 하나 이상의 변형된 리노바이러스 A (Rhinovirus A) 3C 프로테아제 중에서 선택된 변형된 엔테로바이러스(Enterovirus) 3C 프로테아제, 및 야생형 리노바이러스 B (Rhinovirus B) 3C 프로테아제의 T19 및 T20 중에서 선택되는 것과 같은, 또는 I86 및 I87 중에서 선택되는 것과 같은, 또는 I112 및 E114 중에서 선택되는 것과 같은, T19, T20, I86, R87, I114 및 E114 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 갖는 변형된 리노바이러스 B (Rhinovirus B) 3C 프로테아제, 및 야생형 A 형 간염 바이러스(Hepatitis A virus) 3C 프로테아제의 G19 및 E20 중에서 선택되는 것과 같은, 또는 T100과 같은, 또는 T100, L119, T121, M128 및 I130 중에서 선택되는 것과 같은, G17, G18, G19, E20, T100, L19, T121, M128 및 I130 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환들을 갖는 변형된 A 형 간염 바이러스(Hepatitis A virus) 3C 프로테아제와 같은 변형된 헤파토바이러스(Hepatovirus).
전형적으로, 본원에 기술된 상기 야생형 FMDV 3C 프로테아제는 서열 번호 2, 4, 6, 8, 10, 12, 14, 16, 18 및 20의 적어도 하나 중에서 선택된다. 전형적으로, 본원에 기술된 상기 야생형 소 비염 3C 프로테아제는 서열 번호 422 및 434 중에서 선택된다. 전형적으로, 본원에 기술된 상기 야생형 말 비염 3C 프로테아제는 서열 번호 444이다. 전형적으로, 본원에 기술된 상기 야생형 인간 콕사키바이러스 A10 3C 프로테아제는 서열 번호 478이다. 전형적으로, 본원에 기술된 상기 야생형 인간 콕사키바이러스 B1 3C 프로테아제는 서열 번호 494이다. 전형적으로, 본원에 기술된 상기 야생형 인간 콕사키바이러스 B3 3C 프로테아제는 서열 번호 510이다. 전형적으로, 본원에 기술된 상기 야생형 인간 콕사키바이러스 A1 3C 프로테아제는 서열 번호 462이다. 전형적으로, 본원에 기술된 상기 야생형 인간 엔테로바이러스 A71 3C 프로테아제는 서열 번호 536이다. 전형적으로, 본원에 기술된 상기 야생형 인간 간염 바이러스 3C 프로테아제는 서열 번호 538이다. 전형적으로, 본원에 기술된 상기 야생형 인간 폴리오바이러스 3C 프로테아제는 서열 번호 552, 566, 및 580 중에서 선택된다. 전형적으로, 본원에 기술된 상기 야생형 리노바이러스 A (Rhinovirus A) 3C 프로테아제는 서열 번호 604, 618 및 620 중에서 선택된다. 전형적으로, 본원에 기술된 상기 야생형 리노바이러스 B (Rhinovirus B) 3C 프로테아제는 서열 번호 594이다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 2, 4, 6, 8, 10, 12, 14, 16, 18 및 20 중에서 선택된 하나 이상의 야생형 FMDV 3C 프로테아제와 적어도 90%, 적어도 91%와 같은, 적어도 92%와 같은, 적어도 93%와 같은, 적어도 94%와 같은, 적어도 95%와 같은, 적어도 96%와 같은, 적어도 97%와 같은, 적어도 98%와 같은, 적어도 99%의 상동성을 갖는 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 FMDV 3C 프로테아제이다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 422 및 434 중에서 선택된 하나 이상의 야생형 소 비염 3C 프로테아제와 적어도 90%, 적어도 91%와 같은, 적어도 92%와 같은, 적어도 93%와 같은, 적어도 94%와 같은, 적어도 95%와 같은, 적어도 96%와 같은, 적어도 97%와 같은, 적어도 98%와 같은, 적어도 99%를 상동성을 갖는 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 소 비염 3C 프로테아제이다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 444의 상기 야생형 말 비염 A 3C 프로테아제와 적어도 90%, 적어도 91%와 같은, 적어도 92%와 같은, 적어도 93%와 같은, 적어도 94%와 같은, 적어도 95%와 같은, 적어도 96%와 같은, 적어도 97%와 같은, 적어도 98%와 같은, 적어도 99%와 같은 상동성을 갖는 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 말 비염 3C 프로테아제이다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 478의 상기 야생형 인간 콕사키바이러스 A10 3C 프로테아제와 적어도 90%, 적어도 91%와 같은, 적어도 92%와 같은, 적어도 93%와 같은, 적어도 94%와 같은, 적어도 95%와 같은, 적어도 96%와 같은, 적어도 97%와 같은, 적어도 98%와 같은, 적어도 99%와 같은 상동성을 갖는 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 엔테로바이러스 A (Enterovirus A) 3C 프로테아제이다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 536의 상기 야생형 인간 엔테로바이러스 A71 3C 프로테아제와 적어도 90%, 적어도 91%와 같은, 적어도 92%와 같은, 적어도 93%와 같은, 적어도 94%와 같은, 적어도 95%와 같은, 적어도 96%와 같은, 적어도 97%와 같은, 적어도 98%와 같은, 적어도 99%와 같은 상동성을 갖는 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 엔테로바이러스 A (Enterovirus A) 3C 프로테아제이다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 494의 상기 야생형 인간 콕사키바이러스 B1 3C 프로테아제와 적어도 90%, 적어도 91%와 같은, 적어도 92%와 같은, 적어도 93%와 같은, 적어도 94%와 같은, 적어도 95%와 같은, 적어도 96%와 같은, 적어도 97%와 같은, 적어도 98%와 같은, 적어도 99%와 같은 상동성을 갖는 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 엔테로바이러스 B (Enterovirus B) 3C 프로테아제이다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 510의 상기 야생형 인간 콕사키바이러스 B3 3C 프로테아제와 적어도 90%, 적어도 91%와 같은, 적어도 92%와 같은, 적어도 93%와 같은, 적어도 94%와 같은, 적어도 95%와 같은, 적어도 96%와 같은, 적어도 97%와 같은, 적어도 98%와 같은, 적어도 99%와 같은 상동성을 갖는 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 엔테로바이러스 B (Enterovirus B) 3C 프로테아제이다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 462의 상기 야생형 인간 콕사키바이러스 A1 3C 프로테아제와 적어도 90%, 적어도 91%와 같은, 적어도 92%와 같은, 적어도 93%와 같은, 적어도 94%와 같은, 적어도 95%와 같은, 적어도 96%와 같은, 적어도 97%와 같은, 적어도 98%와 같은, 적어도 99%와 같은 상동성을 갖는 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 엔테로바이러스 C (Enterovirus C) 3C 프로테아제이다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 552, 566 및 580 중에서 선택되는 하나 이상의 야생형 인간 폴리오바이러스 3C 프로테아제와 적어도 90%, 적어도 91%와 같은, 적어도 92%와 같은, 적어도 93%와 같은, 적어도 94%와 같은, 적어도 95%와 같은, 적어도 96%와 같은, 적어도 97%와 같은, 적어도 98%와 같은, 적어도 99%와 같은 상동성을 갖는 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 엔테로바이러스 C (Enterovirus C) 3C 프로테아제이다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 604, 618 및 620 중에서 선택되는 하나 이상의 야생형 리노바이러스 A (Rhinovirus A) 3C 프로테아제와 적어도 90%, 적어도 91%와 같은, 적어도 92%와 같은, 적어도 93%와 같은, 적어도 94%와 같은, 적어도 95%와 같은, 적어도 96%와 같은, 적어도 97%와 같은, 적어도 98%와 같은, 적어도 99%와 같은 상동성을 갖는 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 엔테로바이러스(Enterovirus) 3C 프로테아제이다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 594의 상기 야생형 리노바이러스 B (Rhinovirus B) 3C 프로테아제와 적어도 90%, 적어도 91%와 같은, 적어도 92%와 같은, 적어도 93%와 같은, 적어도 94%와 같은, 적어도 95%와 같은, 적어도 96%와 같은, 적어도 97%와 같은, 적어도 98%와 같은, 적어도 99%와 같은 상동성을 갖는 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 엔테로바이러스(Enterovirus) 3C 프로테아제이다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 538의 상기 야생형 인간 간염 바이러스 3C 프로테아제와 적어도 90%, 적어도 91%와 같은, 적어도 92%와 같은, 적어도 93%와 같은, 적어도 94%와 같은, 적어도 95%와 같은, 적어도 96%와 같은, 적어도 97%와 같은, 적어도 98%와 같은, 적어도 99%와 같은 상동성을 갖는 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 A 형 간염 바이러스(Hepatitis A virus) 3C 프로테아제이다.
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 FMDV 3C 프로테아제는 서열 번호 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 178, 180, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 302, 304, 306, 308, 310, 312, 314, 316, 318, 320, 322, 324, 326, 328, 330, 332, 334, 336, 338, 340, 342, 344, 346, 348, 350, 352, 354, 356, 358 및 360 중에서 선택된다.
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 소 비염 3C 프로테아제는 서열 번호 412, 414, 416, 418, 420, 424, 426, 428, 430 및 432 중에서 선택된다.
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 말 비염 3C 프로테아제는 서열 번호 436, 438, 440, 442 및 446 중에서 선택된다.
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 엔테로바이러스 A(Enterovirus A) 3C 프로테아제는 서열 번호 464, 466, 468, 470, 472, 474, 476, 512, 514, 516, 518, 520, 522 및 524 중에서 선택된다.
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 엔테로바이러스 A (Enterovirus A) 3C 프로테아제는 서열 번호 480, 482, 484, 486, 488, 490, 492, 496, 498, 500, 502, 504, 506 및 508 중에서 선택된다.
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 엔테로바이러스 C (Enterovirus C) 3C 프로테아제는 서열 번호 448, 450, 452, 454, 456, 458, 460, 540, 542, 544, 546, 548, 550, 554, 556, 558, 560, 562, 564, 568, 570, 572, 574, 576 및 578 중에서 선택된다.
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 리노바이러스 A (Rhinovirus A) 3C 프로테아제는 서열 번호 596, 598, 600, 602, 604, 606, 608, 610, 612, 614, 616, 618, 620, 622, 624, 626, 628, 630, 632 및 634 중에서 선택된다.
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 리노바이러스 B (Rhinovirus B) 3C 프로테아제는 서열 번호 582, 584, 586, 588, 590 및 592 중에서 선택된다.
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 A 형 간염 바이러스(Hepatitis A virus) 3C 프로테아제는 서열 번호 528, 530, 532, 534, 536, 652 및 654 중에서 선택된다.
일부 구현예들에 있어서, 본원에 기재된 상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 상기 단리된 폴리뉴클레오타이드는 피코르나바이러스 P1 전구체 폴리펩타이드를 코딩하는 적어도 하나의 폴리뉴클레오타이드 서열을 추가 포함한다. 전형적으로, 상기 피코르나바이러스 P1 전구페 폴리펩타이드는 본원에 기재된 것과 같이 상기 피코르나바이러스 속, 종들 및/또는 균주들의 임의의 것으로부터 수득된다.
일부 구현예들에 있어서, 본원에 기재된 상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 상기 단리된 폴리뉴클레오타이드는 3D 펩타이드를 코딩하는 적어도 하나의 폴리뉴클레오타이드 서열을 추가 포함한다.
일부 구현예들에 있어서, 본원에 기재된 상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 상기 단리된 폴리뉴클레오타이드는 융합 폴리펩타이드를 코딩하고, 상기 융합 폴리펩타이드는 적어도 3D 펩타이드를 코딩하는 폴리펩타이드 서열을 포함한다. 전형적으로, 상기 융합 폴리펩타이드는 3CD 폴리펩타이드이다.
일부 구현예들에 있어서, 상기 피코르나바이러스 P1 전구체 폴리펩타이드는 FMDV P1 전구체 폴리펩타이드이다. 일부 구현예들에 있어서, FMDV P1 전구체 폴리펩타이드는 서열 번호 363, 366, 369, 372, 375, 378, 381, 384 및 387의 적어도 하나로부터 선택된다. 일부 구현예들에 있어서, FMDV P1 전구체 폴리펩타이드와 같은, 상기 피코르나바이러스 P1 전구체 폴리펩타이드는 서열 번호 362, 365, 368, 371, 374, 377, 380, 383 및 386에서 적어도 90% 상동성, 적어도 91% 상동성과 같은, 적어도 92% 상동성과 같은, 적어도 93% 상동성과 같은, 적어도 94% 상동성과 같은, 적어도 95% 상동성과 같은, 적어도 96% 상동성과 같은, 적어도 97% 상동성과 같은, 적어도 98% 상동성과 같은, 적어도 99% 상동성과 같은, 100% 상동성과 같은 폴리뉴클레오타이드 서열을 갖는다.
또 다른 측면에 있어서, 본원은, 본원의 제 1 측면의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다. 본원의 후속 측면에 있어서, 상기 제 1 측면의 옵션과 구현예들을 준용 (mutatis mutandis)하여 적용한다.
또 다른 측면에 있어서, 본원은, 본원의 상기 제 1 측면에서 기재된 상기 단리된 폴리뉴클레오타이드를 포함하는 벡터에 관한 것이다. 본원의 후속 측면에 있어서, 상기 일 측면의 옵션과 구현예들을 준용하여 적용한다. 본원의 상기 벡터의 추가적인 옵션과 구현예들은 아래에 기재된다.
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드를 포함하는 본원의 벡터는 본원에 기재된 것과 같이 미니서클 벡터, 플라스미드 벡터, 바큘로바이러스 벡터 (baculovirus vector) , 알파바이러스 벡터 (alphavirus vector), 렌티바이러스 벡터 (lentivirus vector), 복제 결핍 아데노바이러스 벡터 (replication deficient adenovirus vector), 또는 우두 바이러스 벡터 (vaccinia virus vector)이다.
일부 구현예들에 있어서, 본원의 상기 벡터는, 본원에 기재된 상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 폴리뉴클레오타이드에 추가하여 적어도 하나의 목적 단백질 또는 목적 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 추가 포함한다. 예를 들어, 상기 벡터는, 상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 상기 폴리뉴클레오타이드 서열 또는 다른 목적 단백질에 작동가능하게 연결된, 적어도 하나의 프로모터 또는 다른 전사 조절 요소, 적어도 하나의 원핵 생물 또는 진핵 생물 번역 개시 서열 또는 다른 번역 조절 요소, 적어도 하나의 번역 인터럽터 서열, 및/또는 적어도 하나의 리포터 유전자를 추가 포함할 것이다. 다른 목적 단백질은, 예를 들어, VP0, VP3, VP1 및, 선택적으로, 3D, 2A, △1D2A, GLuc 및/또는 SGLuc에 대해 코딩하는 적어도 하나의 폴리뉴클레오타이드 서열과 같은 피코르나바이러스 단백질을 포함한다.
또 다른 측면에 있어서, 본원은 위에서 기재된 상기 벡터를 포함하는 숙주 세포에 관한 것이다. 본원의 후속 측면에 있어서, 상기 제 1 측면의 옵션과 구현예들 및 상기 벡터에 관한 상기 측면을 준용하여 적용한다. 본원의 상기 숙주 세포의 추가적인 옵션과 구현예들은 아래에 기재된다.
일부 구현예들에 있어서, 본원에 기재된 상기 벡터는 본원의 숙주 세포에서 발현된다. 상기 본원 숙주 세포는 진핵 또는 원핵 숙주 세포들 일 수 있다. 본원에 기재된 상기 벡터, 폴리뉴클레오타이드 구조체, 조성물 및/또는 방법에 사용될 수 있는 적합한 진핵 세포는, 사카로마이세스 세레비시아 (Saccharomyces cerevisiae), 피키아 파스토리스 (Pichia pastoris)와 같은 진균류 세포; 애기장대 (Arabidopsis thaliana), 클라미도모나스 레인하티 (Chlamydomonas reinhardtii), 대두 (Glycine max), 니코티아나 벤타미아나 (Nicotiana benthamiana), 재배담배 (Nicotiana tabacum), 벼 (Oryza sativa), 옥수수 (Zea mays)와 같은 식물 세포들; 담배거세미나방종 (Spodoptera frugiperda), 노랑초파리 (Drosophila melanogaster), Sf9, Sf21와 같은 곤충 세포; 예를 들어, HEK-293-T (인간 신장 배아) 세포, 중국 햄스터 난소 [Chinese Hamster Ovary (CHO)] 세포, 아기 햄스터 신장(BHK) 세포, LF-BK (돼지 세포), 및/또는 LF-BK αV/β6 세포인 포유동물 세포와 같은 척추동물 세포를 포함한다.
일부 구현예들에 있어서, 본원에 기재된 상기 벡터는, 예를 들어, FMDV 감염인 피코르나바이러스의 감염에 민감한 동물 세포와 같은 포유동물 세포와 같은, 척추 동물 세포와 같은, 동물세포와 같은 숙주세포에서 발현된다.
적합한 원핵 세포는, 간균 (Bacillus), 락토코코스 (Lactococcus), 스트렙토미세스 (Streptomyces), 로도코커스 (Rhodococcus), 코리네박테리움 (Corynebacterium) 및/또는 미코박테륨 (Mycobacterium)과 같은 그람-양성 원핵 세포, 및 대장균 (Escherichia) 및 슈도모나스(Pseudomonas)와 같은 그람-음성 원핵 세포를 포함한다.
다른 측면에 있어서, 본원은, 본원에 기술된 것과 같이 상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 폴리뉴클레오타이드 또는 벡터, 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의한 단백질 분해에 적합한 약학적으로 허용가능한 부형제, 보조제, 완충제 또는 용액을 포함하는 조성물에 관한 것이다. 이하 본원의 측면에서, 상기 제 1 측면의 옵션과 구현예들 및 상기 벡터에 관한 상기 측면을 준용하여 적용한다.
다른 측면에 있어서, 본원은 또한 피코르나바이러스 P1 전구체 폴리펩타이드를 피코르나바이러스 바이러스 단백질 및/또는 바이러스-유사 입자(VLPs)로 가공하는 방법에 관한 것으로서, 상기 방법은 하기를 포함한다: 적합한 배지에서 본원의 단리된 폴리뉴클레오타이드를 포함하는 숙주 세포 및/또는 본원의 단리된 폴리뉴클레오타이드를 포함하는 벡터를 배양하고; VP0, VP1, VP2, VP3 및 VP4 및/또는 VLPs 중에서 선택되는 적어도 하나의 바이러스 단백질을 회수함. 본원의 후속하는 측면에 있어서, 상기 제 1 측면의 옵션과 구현예들을 준용하여 적용한다. 상기 벡터 측면 및 숙주 세포 측면의 상기 옵션과 구현예들 또한 준용하여 적용한다. 본원의 추가적인 옵션과 구현예들은 아래에 기재된다.
일부 구현예들에 있어서, 적어도 VP0, VP1 및 VP3이 회수된다. 일부 구현예들에 있어서, 회수되는 상기 VLPs는 FMDV VLPs이다.
일부 구현예들에 있어서, 피코르나바이러스 바이러스 단백질 및/또는 VLPs에서 피코르나바이러스 P1 전구체 폴리펩타이드를 가공하기 위한 방법은, 분비된 루시퍼라아제(luciferase)의 양을 측정함으로써, FMDV P1 전구체 폴리펩타이드 발현과 같은, 변형된 FMDV 3C 프로테아제 또는 피코르나바이러스 P1 전구체 폴리펩타이드 발현과 같은, 피코르나바이러스 3C 프로테아제 활성을 모니터링하는 것을 추가 포함한다.
상기 방법의 일부 구현예들에 있어서, 본원의 숙주 세포에 의해 발현된, FMDV P1 전구체 폴리펩타이드와 같은 상기 피코르나바이러스 P1 전구체 폴리펩타이드의 적어도 90%, 또는 적어도 95%가, 본원에 기재된 변형된 FMDV 피코르나바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 의해 절단된다.
상기 방법의 일부 구현예들에 있어서, 대조군 피코르나바이러스 3C 프로테아제를 발현하는 다른 상동성 숙주 세포와 비교하여, 하나 이상의 숙주 세포 단백질의, 5% 미만과 같은, 2% 미만과 같은, 10% 미만이 본원에 기술된 변형된 FMDV 3C 프로테아제와 같은, 피코르나바이러스 3C 프로테아제에 의해 절단된다.
상기 방법의 일부 구현예들에 있어서, FMDV P1 전구체 폴리펩타이드와 같은, 본원에 기재된 피코르나바이러스 P1 전구체 폴리펩타이드는, 대조군 피코르나바이러스 3C 프로테아제를 발현하는 다른 상동성 숙주 세포로부터의 양보다 숙주 세포에서 더 많이 발현되거나 또는 더 많이 회수된다.
상기 방법의 일부 구현예들에 있어서, VP0, VP1, VP2, VP3, VP4 및/또는 VLPs는, 대조군 피코르나바이러스 3C 프로테아제에서의 양보다 숙주 세포에서 더 많은 양이 발현되거나 또는 회수된다.
상기 방법의 일부 구현예들에 있어서, 본원에 기재된 숙주 세포에서 본원에 기재된 벡터는, 변형된 FMDV 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제, 2A 또는 다른 변역 인터럽터 서열, 및 N-말단 메티오닌 잔기가 없는 FMDV P1 전구체 폴리펩타이드와 같은 피코르나바이러스 P1 전구체 폴리펩타이드, 및 선택적으로, 분비될 수 있는 GLuc, SGLuc 또는 다른 루시퍼라아제 중 적어도 하나를 코딩한다.
상기 방법의 일부 구현예들에 있어서, 본원에 기재된 숙주 세포에서 벡터는, 본원에 기재된 변형된 FMDV 3C 프로테아제와 같은 변형된 피코르나바이러스 3C 프로테아제, 피코르나바이러스 3D 폴리머라아제, 2A 또는 다른 번역 인터럽터 서열, 및 N-말단 메티오닌 잔기 내, FMDV P1 전구체 폴리펩타이드와 같은 피코르나바이러스 P1 전구체 폴리펩타이드, 및 선택적으로, 분비될 수 있는 GLuc, SGLuc 또는 다른 루시퍼라아제 중 적어도 하나를 코딩한다.
상기 방법의 일부 구현예들에 있어서, 본원의 숙주 세포는, 본원에 기재된 변형된 FMDV 3C 프로테아제와 같은 변형된 피코르나바이러스 3C 프로테아제, 본원에 기재된 FMDV P1 전구체 폴리펩타이드와 같은 피코르나바이러스 P1 전구체 폴리펩타이드, 및 분비될 수 있는 GLuc, SGLuc 또는 다른 루시퍼라아제 중 하나 이상, 및 선택적으로 3D, 2A, △1D2A, 또는 다른 번역 인터럽터 서열 중 하나 이상을 포함하는 융합 단백질을 발현하는 벡터를 포함한다.
상기 방법의 일부 구현예들에 있어서, 본원의 숙주 세포는, 본원에 기재된 변형된 FMDV 3C 프로테아제와 같은 변형된 피코르나바이러스 3C 프로테아제, 본원에 기재된 FMDV P1 전구체와 같은 피코르나바이러스 P1 전구체 폴리펩타이드, 피코르나바이러스 3D 폴리머라아제, 및 분비될 수 있는 GLuc, SGLuc 또는 다른 루시퍼라아제 중 하나 이상, 및 선택적으로, 2A, △1D2A, 또는 다른 번역 인터럽터 서열 중 하나 이상을 포함하는 융합 단백질을 발현하는 벡터를 포함한다.
본원은 또한, 본원에 기재된 것과 같이, 변형된 피코르나바이러스 3C 프로테아제 및 P1 전구체 폴리펩타이드를 코딩하는 벡터 또는 폴리뉴클레오타이드 구조체(construct)를 그것을 필요로 하는 대상에게 투여하는 것을 포함하는, 피코르나바이러스에 대한 면역 반응을 유도하는 방법에 관한 것이다.
본원은 또한, 본원에 기재된 것과 같이, 상기 변형된 피코르나바이러스 3C 프로테아제 및 P1 전구체 폴리펩타이드를 코딩하는 단리된 폴리뉴클레오타이드의 주입을 통한 진핵 세포 및/또는 원핵 세포에서 생성되는, 본원에 기재된 것과 같은 VP0, VP1, VP2, VP3 및 VP4 및/또는 VLPs를 필요로 하는 대상에게 투여하는 것을 포함하는, 피코르나바이러스에 대한 면역 반응을 유도하는 방법에 관한 것이다.
본원의 후속 측면에 있어서, 상기 일 측면의 옵션과 구현예들을 준용하여 적용한다.
도 1은, 단일 개방-판독 프레임 (open-reading frame, ORF) 및 단일 ORF에서 바이러스 폴리프로테인을 코딩하는 피코르나바이러스 RNA 게놈의 연관된 유전적 요소를 나타낸 것이다. 도 1은, 또한 중간 전구체 폴리펩타이드로, 그리고 및 궁극적으로 개별적인 바이러스 단백질로 상기 바이러스 폴리프로테인을 가공하는 것을 나타낸 것이다.
도 2는, 발명의 설명에 기술한 것과 같이, FMDV 3C 프로테아제 결정 구조의 다이아그램을 나타낸 것이다.
도 3은, 예시적으로 야생형 FMDV 균주 Asia Lebanon 89 (서열 번호 6)의 3C 프로테아제의 알려진 2차 구조 및 그것의 아미노산 서열 내에 위치 46, 84 및 163에서 촉매성 잔기의 트리아드의 상대적 위치들을 나타낸다.
도 4는, 발명의 설명 및 실시예 3에 기재된 것과 같이, FMDV, 소 비염 A (Bovine rhinitis A), 소 비염 B (Bovine rhinitis B) 및 말 비염 A (Equine rhinitis A)로부터 야생형 3C 프로테아제의 정렬을 나타낸 것이다. 선택된 2차 구조는 또한 동정된다.
도 5는, 발명의 설명 및 실시예 3에 기재된 것과 같이, FMDV, 콕사키바이러스 (coxsackievirus) A10 및 콕사키바이러스(coxsackievirus) A1로부터 야생형 3C 프로테아제의 정렬을 나타낸 것이다. 선택된 2차 구조는 또한 동정된다.
도 6은, 발명의 설명 및 실시예 3에 기재된 것과 같이, FMDV 및 인간 엔테로바이러스(Enterovirus) A71로부터 야생형 3C 프로테아제의 정렬을 나타낸 것이다. 선택된 2차 구조들은 또한 동정된다.
도 7은, 발명의 설명 및 실시예 3에 기재된 것과 같이, FMDV, 콕사키바이러스 B3 및 콕사키바이러스 B1로부터 야생형 3C 프로테아제의 정렬을 나타낸 것이다. 선택된 2차 구조들은 또한 동정된다.
도 8은, 발명의 설명 및 실시예 3에 기재된 것과 같이, FMDV, 폴리오바이러스 1, 폴리오바이러스 2 및 폴리오바이러스 3으로부터 야생형 3C 프로테아제의 정렬을 나타낸 것이다. 선택된 2차 구조들은 또한 동정된다.
도 9는, 발명의 설명 및 실시예 3에 기재된 것과 같이, FMDV, 인간 리노바이러스 A2, 인간 리노바이러스 A89 및 인간 리노바이러스 A20으로부터 야생형 3C 프로테아제의 정렬을 나타낸 것이다. 선택된 2차 구조들은 또한 동정된다.
도 10은, 발명의 자세한 설명 및 실시예 3에 기재된 것과 같이, FMDV 및 인간 리노바이러스 B14로부터 야생형 3C 프로테아제의 정렬을 나타낸 것이다. 선택된 2차 구조들은 또한 동정된다.
도 11은, 발명의 자세한 설명 및 실시예 3에 기재된 것과 같이, FMDV 및 A 형 간염 바이러스(Hepatitis A virus)로부터 야생형 3C 프로테아제의 정렬을 나타낸 것이다. 선택된 2차 구조들은 또한 동정된다.
도 12는, 발명의 설명 및 실시예 3에 기재된 것과 같이, FMDV, 콕사키바이러스(coxsackievirus) A10, 콕사키바이러스(coxsackievirus) A1, 폴리오바이러스 1, 폴리오바이러스 2, 폴리오바이러스 3, 인간 리노바이러스 A2, 인간 리노바이러스 A89, 인간 리노바이러스 A20, 인간 리노바이러스 B14, 인간 리노바이러스 A71, 콕사키바이러스(coxsackievirus) B1, A 형 간염 바이러스, 소 비염 A (Bovine rhinitis A), 소 비염 B (Bovine rhinitis B) 및 말 비염 A (Equine rhinitis A) 로부터 야생형 3C 프로테아제 일부의 정렬을 나타낸 것이다. 선택된 2차 구조들은 또한 동정된다.
도 13a 내지 13d: 도 13a는, 실시예 2A에 기재된 것과 같이 아미노산 123 내지 128에 의해 형성된 사이드 체인을 나타내는 FMDV 3C 프로테아제의 결정 구조의 일부를 나타낸 것이다. 도 13b는, 실시예 2A 및 2B에 기재된 것과 같이, FMDV P1 폴리펩타이드 (O1MP1), 3C 프로테아제 및 마커 (△1D2A-GLuc △1M)를 코딩하는 pJJP 발현 플라스미드의 맵(map)을 나타낸 것이다. 도 13c 및 도 13d는 각각, 실시예 2A 및 2B에 기재된 것과 같이, 잔기 123 내지 128에서 프롤린 치환들을 포함하는 FMDV 3C 프로테아제 돌연변이체, 야생형 FMDV 3C 프로테아제 및 잔기 163에서 시스테인의 위치에 알라닌을 포함하는 FMDV 3C 프로테아제 돌연변이체를 코딩하는 pJJP 플라스미드를 형질주입한 HEK293T 세포로부터 수득된, 세포 용해물(lysates)의 루시퍼라아제 판독 값 및 웨스턴 블롯 (Western blot)을 나타낸 것이다.
도 14는, 실시예 2B에 기재된 것과 같이, 대장균(E.coli) 콜로니 성장에 대한 야생형 3C 프로테아제 발현 및 3C 프로테아제 돌연변이체, L23P, L43H 및 T100P의 효과 평가를 위해, 카베니실린 (carbenicillin) (+CARB) 또는 카베니실린/이소프로필 β-D-1-티오갈락토피라노시드 (thiogalactopyranoside) (+CARB/IPTG) 한천(agar) 성장 배지에서 형질전환된 대장균(E.coli) 박테리아를 나타낸 것이다.
도 15a 내지 15b: 도 15a 및 도 15b는, 실시예 2B에 기재된 것과 같이, 잔기 22, 23, 99, 100 및 127에서 프롤린 치환을 포함하는 FMDV 3C 프로테아제 돌연변이체, 야생형 FMDV 3C 프로테아제 및 잔기 163에서 시스테인의 위치에 알라닌을 포함하는 FMDV 3C 프로테아제 돌연변이체를 코딩하는 pJJP 플라스미드로 형질주입된 HEK293T 세포로부터 수득된 루시퍼라아제 판독 값 및 세포 용해물의 웨스턴 블롯을 각각 나타낸 것이다.
도 16은, 야생형 FMDV 3C 프로테아제에서 잔기 T100, V124 및 L127의 위치 및 상기 잔기들을 포함하는 상기 구조들의 상대적인 위치를 나타낸 것이다.
도 17a 내지 17b: 도 17a 및 도 17b는, 실시예 2C에 기재된 것과 같이, 잔기 22, 100 및 127 및 잔기 22, 100 및 124에서 3중(triple) 프롤린 치환들을 포함하는 FMDV 3C 프로테아제 돌연변이체, 야생형 FMDV 3C 프로테아제 및 잔기 163에서 시스테인의 위치에 알라닌을 포함하는 FMDV 3C 프로테아제 돌연변이체를 코딩하는 pJJP 플라스미드로 형질주입된 HEK293T 세포로부터 수득된 루시퍼라아제 판독 값 및 세포 용해물의 웨스턴 블롯을 각각 나타낸 것이다.
도 18은, 실시예 2D에 기재된 것과 같이, 잔기 22, 23, 100, 124, 125, 126, 127에서 3Cpro 프롤린 돌연변이, 및 잔기 127와 잔기 22 또는 124의 이중 돌연변이 조합을 포함하는 pJJP 플라스미드로 형질주입된 HEK293-T 세포에서 숙주 eIF4AI의 가공을 나타내는 웨스턴 블롯을 나타낸 것이다.
도 19a 내지 19d: 도 19a는, 실시예 2G(ⅰ)에 기재된 것과 같이, C163 잔기를 포함하는 상기 루프와 비교하여 야생형 FMDV 3C 프로테아제에서 상기 B2-A2 β 시트의 구조를 나타낸 것이다. 도 19b는, 또한 실시예 2G(ⅰ)에 기재된 것과 같이, Y162와 비교하여 잔기 V119, N121, V124, 및 L127에 대한 상기 프로테아제 사이드 체인 구조를 나타낸 것이다. 도 19c는, 상기 기질 결합 포켓(binding pocket)과 비교하여, 상기 N-말단 α 헬릭스 및 A1 β 가닥을 포함하는, 상기 구조 스패닝(spanning) 잔기 7 내지 28을 나타낸 것이다. 잔기 I22 및 L23의 사이드 체인 또한 나타낸다. 실시예 2G(ⅱ) 참조. 도 19d는, 실시예 2G(ⅲ)에 기재된 것과 같이, 잔기 I99 및 T100에 연결되거나 근접한 구조들을 나타낸 것이다.
도 20은, 실시예 2G(ⅰ)에 기재된 것과 같이, 6 개의 피코르나바이러스로 부터 3C 프로테아제의 결정 구조체들에서 상기 촉매성 시스테인에 인접한 잔기에 근접한(proximity) 사이드 체인을 갖는 상기 잔기들을 나타낸 것이다.
도 21은, 실시예 2G(ⅱ)에 기재된 것과 같이, FMDV 3C 프로테아제의 잔기 I22 및 L23에 구조적 동족성을 갖는 5 개의 피코르나바이러스에서 상기 잔기들을 나타낸 것이다.
도 22는, 실시예 2G(ⅲ)에 기재된 것과 같이, FMDV 3C 프로테아제의 잔기 T100에 구조적 동족성을 갖는 5 개의 피코르나바이러스에서 상기 잔기들을 나타낸 것이다.
도 23은, 실시예 2E에 기재된 것과 같이, 잔기 I22, L23, T100, V124, G125, R126, L127에서 FMDV 3Cpro 프롤린 돌연변이 및 이중 돌연변이체 조합 I22/L127 및 V124/L127을 포함하는 pJJP 플라스미드로 형질주입된 CHO-K1 (어두운 회색) 및 BHK-21 (밝은 회색) 세포로부터 루시퍼라아제 판독 값을 나타낸 것이다.
도 24a 내지 24c: 도 24a는, 실시예 2E에 기재된 것과 같이, 잔기 I22, L23, T100, V124, G125, R126, L127에서 FMDV 3Cpro 프롤린 돌연변이 및 이중 돌연변이체 조합들 I22/L127 및 V124/L127을 포함하는 pJJP 플라스미드로 형질주입된 CHO-K1 및 BHK-21 세포로부터 세포 용해물의 웨스턴 블롯을 나타낸 것이다. 도 24b는, 실시예 2E에 기재된 것과 같이, 상기 L127P 돌연변이 및 O1 Manisa P1 폴리펩타이드를 포함하는 pJJP 플라스미드로 형질주입된 CHO-K1 세포들에서 VLP 어레이의 전자 현미경 이미지를 나타낸 것이다. 도 24c는, 실시예 2E에 기재된 것과 같이, 상기 V124P 돌연변이 및 O1 Manisa P1 폴리펩타이드를 포함하는 pJJP 플라스미드로 형질주입된 BHK-21 세포들에서 VLP 어레이의 전자 현미경 이미지를 나타낸 것이다.
도 25는, 실시예 4에 기재된 것과 같이, 3D 폴리머라아제로 융합된 폴리오바이러스 3C 프로테아제 및 3C 프로테아제를 발현하기 위해 사용되는 상기 발현 시스템을 나타낸 것이다.
도 26은, 실시예 4에 기재된 것과 같이, 항-Gluc 항체를 사용하여 가우시아(Gaussia) 루시퍼라아제 ("Gluc") 발현에 대한 2 개의 돌연변이체, R87P 및 L102P의 발현의 효과를 나타낸 것이다.
정의
본원에 사용된, 용어 "단리된(isolated)"은 천연적으로 연관된 적어도 하나의 성분으로부터 제거된 폴리뉴클레오타이드, 폴리펩타이드 또는 다른 성분을 의미한다. 예를 들어, 단리된 폴리뉴클레오타이드, 폴리펩타이드 또는 다른 성분은, 정제하거나 정제하지 않은 것으로서, 자연에서 전형적으로 발견되는 세포의 외부에 존재할 수 있다. 추가적으로 또는 대안적으로, 상기 단리된 성분은, 예를 들어, 자연에서 전형적으로 근접하게 존재하는 뉴클레오타이드 서열, 또는 전형적으로 근접하지 않은 인접한 [또는 인접한(contiguous)] 뉴클레오타이드 서열로부터 분리되는, 자연적으로 발견되는 것과 다른 콘텍스트(context)에서 발견된다. 선택적으로, 폴리뉴클레오타이드 또는 폴리펩타이드와 같은 단리된 성분에 대해 세포 용해(lysis), 추출, 원심분리, 정제 등과 같은 하나 이상의 농축 또는 정제 과정이 실시될 수 있다.
"뉴클레오타이드"는 DNA 및 RNA와 같은 핵산의 모노머, 또는 서브유닛으로서 작용하는 유기체 분자를 의미한다. 뉴클레오타이드는 핵산의 빌딩 블럭이고, 질소성 염기[예: A(아데닌), G(구아닌), C(시토신), T/U(티민/우라실)], 5탄당(리보오스 또는 디옥시리보오스), 및 적어도 하나의 인산기로 구성된다. 따라서, 뉴클레오시드와 인산기는 뉴클레오타이드를 형성한다. 폴리뉴클레오타이드 서열에서 뉴클레오타이드는 일반적으로 그것들의 질소성 염기에 기초하여 표시된다.
"뉴클레오타이드 서열", "폴리뉴클레오타이드 서열" 또는 "핵산 서열"은 DNA (GACT 사용) 또는 RNA 분자 (GACU 사용) 내에 뉴클레오타이드 또는 핵산의 순서를 나타내는 일련의 문자이다. DNA 분자, RNA 분자 또는 다른 폴리뉴클레오타이드 서열은 단일 또는 이중 가닥일 수 있고, 게놈, 재조합, 합성, 전사, PCR 생성물, 증폭 생성물, mRNA 또는 cDNA일 수 있다. "뉴클레오타이드 서열", "폴리뉴클레오타이드 서열" 또는 "핵산 서열"은 선택적으로 변형된 염기 또는 변형된 백본(backbone)을 포함할 수 있다. 상기 용어들은 또한 센스(sense) 또는 안티센스(antisense) 배향 및 이들의 보체(complement) 서열을 또한 포함하는 것을 의미한다.
"재조합 폴리뉴클레오타이드"는, 예를 들어, 상기 폴리뉴클레오타이드가 자연에서 발견되지 않는 뉴클레오타이드 서열을 포함하는 것과 같은, 천연 상태에 있지 않는 폴리뉴클레오타이드이다. 예를 들어, 목적 서열은 벡터에서 복제될 수 있고, 또는 하나 이상의 추가 핵산으로 재조합될 수 있다.
본원에서 사용된, 용어 "피코르나바이러스"는 정이십면체 (icosahedral) 캡시드를 갖는 작고, 세포질, 플러스-가닥 RNA (~7.0-8.5 kb) 바이러스의 과(family)를 나타내는 비-외피형 바이러스를 의미한다. 상기 과의 속(Genera)은 아프토바이러스 (Aphthovirus), 아쿠아마바이러스 (Aquamavirus), 아비헤파토바이러스 (Avihepatovirus), 카디오바이러스 (Cardiovirus), 코사바이러스 (Cosavirus), 디시피바이러스 (Dicipivirus), 엔테로바이러스 (Enterovirus), 에르보바이러스(Erbovirus), 헤파토바이러스 (Hepatovirus), 코부바이러스 ( Kobuvirus ), 메기바이러스 (Megrivirus), 파레코바이러스 (Parechovirus), 피스세바이러스 (Piscevirus), 살리바이러스 (Salivirus), 사펠로바이러스 (Sapelovirus), 세네카바이러스(Senecavirus), 테스코바이러스 (Teschovirus) 및 트레모바이러스(Tremovirus)를 포함한다. 피코르나바이러스의 바이러스 게놈은 전체적으로 구조 단백질 영역, P1, 및 비-구조 단백질 영역, P2 및 P3을 포함하는 단일 폴리프로테인을 코딩하는 하나의 개방 판독 프레임을 포함한다 (도 1 참조). 상기 폴리프로테인으로부터 성숙한 기능성 단백질의 방출은 주로 3C 프로테아제를 포함하는 바이러스 프로테아제에 의해 매개된다.
본원에 사용된, 용어 "아프토바이러스(Aphthovirus)"는, 소 비염 A 바이러스 (Bovine rhinitis A virus), 소 비염 B 바이러스 (Bovine rhinitis B virus), 말 비염 A 바이러스 (Equine rhinitis A virus) 및 FMDV와 같은 종들을 포함하는, 상기 피코르나바이러스 과의 속을 의미한다.
본원에 사용된, 용어 "소 비염(Bovine rhinitis)"은 소 비염 A 바이러스 (Bovine rhinitis A virus) (BRAV) 및 소 비염 B 바이러스 (Bovine rhinitis B virus) (BRBV)를 포함하는 모든 소 비염 종들을 의미한다. 상기 용어는, 각각, 소 비염 1 & 3으로 알려진 소 비염 A 바이러스 1 및 소 비염 A 바이러스 2를 포함하는 소 비염 A 바이러스 (Bovine rhinitis A virus) 혈청형을 포함하는 것을 또한 의미한다.
본원에 사용된, 용어 "말 비염(Equine rhinitis)"은 또한 말 비염 1로 알려진, 말 비염 A 바이러스 (Equine rhinitis A virus) (ERAV)를 포함하는 임의의 말 비염 종들을 의미한다.
본원에 사용된, "인간 엔테로바이러스 A(Human enterovirus A)"로 또한 알려진 용어 "엔테로바이러스 A (Enterovirus A)"는 상기 엔테로바이러스(Enterovirus) 유전자의 종들을 의미한다. 용어 "엔테로바이러스 A (Enterovirus A)"는 CV-A2, CV-A3, CV-A4, CV-A5, CV-A6, CV-A7, CV-A8, CV-A10, CV-A12, CV-A14 & CV-A16과 같은 콕사키바이러스(coxsackievirus) 혈청형들 및 EV-A71, EV-A76, EV-A89, EV-A90, EV-A91, EV-A92, EV-A114, 및 EV-A119와 같은 엔테로바이러스(EV) 혈청형을 포함하는 상기 종들의 혈청형을 포함하는 것을 의미한다. SV19, SV43, SV46과 같은 시미언 바이러스(Simian virus) 혈청형 및 BA13과 같은 바분 (Baboon) 혈청형이 또한 상기 종들에 포함된다.
본원에 사용된, 또한 "인간 엔테로바이러스 B (Human enterovirus B)"로 알려져 있는 용어 "엔테로바이러스 B (Enterovirus B)"는 상기 엔테로바이러스(Enterovirus) 속의 종들을 의미한다. 용어 "엔테로바이러스 B (Enterovirus B)"는 CV-B1, CV-B2, CV-B3, CV-B4, CV-B5, CV-B6 및 CV-A9와 같은 콕사키바이러스(coxsackievirus, CV) 혈청형들 및 EV-B69, EV-B73, EV-B74, EV-B75, EV-B77, EV-B78, EV-B79, EV-B80, EV-B81, EV-B82, EV-B83, EV-B84, EV-B85, EV-B86, EV-B87, EV-B88, EV-B93, EV-B97, EV-B98, EV-B100, EV-B101, EV-B106, EV-B107, EV-B110 및 SA5과 같은 엔테로바이러스(EV) 혈청형들을 포함하는 상기 종들의 혈청형을 포함하는 것을 의미한다. E-1, E-2, E-3, E-4, E-5, E-6, E-7, E-9, E-11, E-12, E-13, E-14, E-15, E-16, E-17, E-18, E-19, E-20, E-21, E-24, E-25, E-26, E-27, E-29, E-30, E-31, E-32, & E-33과 같은 에코바이러스 (Echovirus, E) 혈청형들 또한 상기 종들에 포함된다.
본원에 사용된, "인간 엔테로바이러스 C (Human enterovirus C)"라고도 알려진, 용어 "엔테로바이러스 C (Enterovirus C)"는 상기 엔테로바이러스(Enterovirus) 속의 종들을 의미한다. 용어 "엔테로바이러스 C (Enterovirus C)"는 CV-A1, CV-A11, CV-A13, CV-A17, CV-A19, CV-A20, CV-A21, CV-A22 및 CV-A24와 같은 콕사키바이러스(CV) 혈청형들 및 EV-C95, EV-C96, EV-C99, EV-C102, EV-C104, EV-C105, EV-C109, EV-C116, EV-C117 및 EV-C118와 같은 엔테로바이러스(EV) 혈청형들을 포함하는 상기 종들의 혈청형을 포함하는 것을 의미한다. PV1, PV2 및 PV3을 포함하는 폴리오바이러스(poliovirus) 혈청형 또한 상기 종들에 포함된다.
본원에 사용된, 용어 "인간 콕사키바이러스(human coxsackievirus)"는 인간을 감염시키는 상기 콕사키바이러스 혈청형들을 포함하는 것을 의미한다. 이러한 혈청형들은 CV-A10과 같은 엔테로바이러스 A (Enterovirus A) 혈청형들, CV-B1 및 CV-B3과 같은 엔테로바이러스 B (Enterovirus B) 혈청형들; 및 CV-A1과 같은 엔테로바이러스 C (Enterovirus C) 혈청형들을 포함한다.
본원에 사용된, 용어 "인간 폴리오바이러스(human poliovirus)"는 인간을 감염시키는 상기 폴리오바이러스(poliovirus) 혈청형들을 포함하는 것을 의미한다. 이러한 혈청형들은 폴리오바이러스-(PV-) 1, PV-2 및 PV-3과 같은 엔테로바이러스 C (Enterovirus C) 를 포함한다.
본원에 사용된, "인간 리노바이러스 A (Human rhinovirus A)"라고도 알려진 용어 "리노바이러스(Rhinovirus) A"는 상기 엔테로바이러스(Enterovirus) 속의 종들을 의미한다. 용어 "리노바이러스 A (Rhinovirus A)"는 HRV-A1, HRV-A2, HRV-A7, HRV-A8, HRV-A9, HRV-A10, HRV-A11, HRV-A12, HRV-A13, HRV-A15, HRV-A16, HRV-A18, HRV-A19, HRV-A20, HRV-A21, HRV-A22, HRV-A23, HRV-A24, HRV-A25, HRV-A28, HRV-A29, HRV-A30, HRV-A31, HRV-A32, HRV-A33, HRV-A34, HRV-A36, HRV-A38, HRV-A39, HRV-A40, HRV-A41, HRV-A43, HRV-A44, HRV-A45, HRV-A46, HRV-A47, HRV-A49, HRV-A50, HRV-A51, HRV-A53, HRV-A54, HRV-A55, HRV-A56, HRV-A57, HRV-A58, HRV-A59, HRV-A60, HRV-A61, HRV-A62, HRV-A63, HRV-A64, HRV-A65, HRV-A66, HRV-A67, HRV-A68, HRV-A71, HRV-A73, HRV-A74, HRV-A75, HRV-A76, HRV-A77, HRV-A78, HRV-A80, A81, HRV-A82, HRV-A85, HRV-A88, HRV-A89, HRV-A90, HRV-A94, HRV-A95, HRV-A96, HRV-A98, HRV-A100, HRV-A101, HRV-A102 및 HRV-A103와 같은 인간 리노바이러스(HRV) 혈청형을 포함한다.
본원에 사용된, "인간 리노바이러스 B (Human rhinovirus B)"라고도 알려진, 용어 "리노바이러스 B (Rhinovirus B)"는 상기 엔테로바이러스(Enterovirus) 속의 종들을 의미한다. 용어 "리노바이러스 B (Rhinovirus B)"는 HRV-B3, HRV-B4, HRV-B5, HRV-B6, HRV-B14, HRV-B17, HRV-B26, HRV-B27, HRV-B35, HRV-B37, HRV-B42, HRV-B48, HRV-B52, HRV-B69, HRV-B70, HRV-B72, HRV-B79, HRV-B83, HRV-B84, HRV-B86, HRV-B91, HRV-B92, HRV-B93, HRV-B97 및 HRV-B99와 같은 인간 리노바이러스 (HRV) 혈청형을 포함한다.
본원에 사용된, 용어 "A 형 간염 바이러스(Hepatitis A virus)"는 상기 헤파토바이러스(Hepatovirus) 속에 속하는 바이러스 종들을 의미하고 및 A 형 간염 바이러스 1을 포함하는 상기 A 형 간염 바이러스(Hepatitis A virus) 내의 모든 혈청형들을 포함한다.
본원에 사용된, 용어 "3CPRO", "3C 프로테아제", "피코르나바이러스 3CPRO" 또는 "피코르나바이러스 3C 프로테아제"는 피코르나바이러스 종들에서 발견되는 시스테인 프로테아제를 의미하고, 전형적으로 그의 활성 부위 내에 보존된 시스테인-히스티딘-아스파라긴 산/글루타민 산 촉매성 트리아드 또는 시스테인-히스티딘 다이아드(dyad) [예: A 형 간염 바이러스 (Hepatitis A)]를 포함한다. 상기 촉매성 트리아드 또는 다이아드는 전형적으로 공유성의 중간체를 형성하기 위해 기재를 공격하는 친핵체 (전형적으로, 시스테인)를 분극화하고 활성화하는 전하-릴레이 네트워크를 형성하고, 이것은 그 후 유리(free) 효소를 재생하기 위해 가수분해된다. 3CPRO가 상기 폴리프로테인의 상기 구조 및 비구조 영역에서 돌연변이 절단을 수행하고 글루타민-글리신/세린/알라닌/발린/히스티딘/아르기닌 및 글루타민 산-세린/글리신/아르기닌/메티오닌에서 현저한 기질 선호를 가진다.
본원에 사용된, "FMDV 3C 프로테아제"는 상기 FMDV 종들로부터 피코르나바이러스 3C 프로테아제를 의미한다. 전체적으로, FMDV 3C 프로제아제는 약 23.1-kDa 분자량을 갖고 213 개 아미노산을 포함하는 시스테인 프로테아제이다. 상기 FMDV 3C 프로테아제의 활성 부위에서 상기 시스테인-히스티딘-아스파라긴 산 촉매성 트리아드는 잔기 H46, D84 및 C163에 의해 형성된다. 구조적으로, FMDV 3C 프로테아제는 N-말단 β-배럴 도메인 및 C-말단 β-배럴 도메인을 포함하는 키모트립신(chymotrypsin)-유사 접힘(fold)을 채용한다(도 2 참조). 상기 β-배럴 도메인들의 각각은 펩타이드 또는 기질 결합 틈(cleft)을 형성하기 위해 함께 팩킹되는 네 가닥 역-평행 β-시트의 한 쌍으로 구성된다. 상기 β-시트는 B1, A1, D1, E1 β-가닥 및 B1, C1, F1, E1 β-가닥 (N-말단 도메인) 및 B2, A2, D2, E2 β-가닥 및 B2, C2, F2, E2 β-가닥 (C-말단 도메인)으로 구성되어 있고, 각각의 도메인의 상기 B 및 E 가닥은 상기 두 개의 β-시트에 기여한다. 상기 N-말단 및 C-말단 β-배럴 도메인들의 다른 2차 구조들은, 예를 들어, A1-B1, A2-B2, B1-C1, B2-C2, C1-D1, C2-D2, E1-F1, E2-F2 루프와 같은, 상기 β-가닥들을 연결하는 루프(loop) 또는 회전(turn)을 포함한다. N-말단 α-헬릭스 ("αN") 및 C-말단 α-헬릭스 ("αC")는 또한 FMDV 3C 프로테아제들 내에서 발견되는 2차 구조들이다.
상기 두 개의 β-배럴 도메인에 추가하여, 상기 FMDV 3C 프로테아제는 두 개의 짧은 역-평행 β-가닥들의 작은 β-시트, 및 상기 두 개의 β-가닥들을 연결하는 꼭지점 루프(apical loop)를 갖는 β-가닥의 형성에서 다른 현저한 3차 구조를 보유한다. 도 2에서 볼 수 있는 것과 같이, 상기 β-가닥은 상기 기재 결합 틈 및 활성 부위에 대하여 접히고 기질 인식 및 특이성에 기여한다. 도 3에 나타낸 것과 같이, 상기 두 개의 β-가닥들 및 상기 꼭지점 루프를 포함하는 상기 β-가닥은 상기 FMDV 3C 프로테아제의 잔기들 138 내지 150에 의해 형성된다.
본원에 사용된, "1차 구조(primary structure)"는 단백질의 선형 아미노산 서열을 의미하며, 이것은 화학적으로 펩타이드 결합들에 의해 연결된 아미노산들로 구성된 폴리펩타이드 체인이다.
본원에 사용된, "2차 구조"는, 단백질의 국소 단편(local segments)의 3차원 형태를 의미한다. 2차 구조는, 펩타이드의 3차원 구조로의 접힘을 용이하게 하는 알파-헬릭스, 베타-시트, 루프, β 가닥들, 및 다른 형태를 형성하는 폴리펩타이드 체인 내에 아미노산의 탄소, 산소 및 NH 기 사이에서 발생하는 상호 작용에 기인된다. 2차 구조들의 특정 예는, B2 β 가닥 및 A2 β 가닥으로부터 형성된 A2-B2 β 시트들, 및 B1 β 가닥 및 A1 β 가닥으로부터 형성된 A1-B1 β 시트들과 같은, β-시트들을 포함한다. 다른 예들은, FMDV와 같은 아프토바이러스(Aphthovirus) 종들에서 존재할 수 있는 B2' β 가닥을 포함한다. 도 13a 참조. 또한, 상기 아미노산 서열에서 그것들의 상응하는 잔기들을 갖는 FMDV 3C 프로테아제의 예시적인 2차 구조들을 정렬하는, 도 3 참조.
본원에 사용된. "변경된 2차 구조"는, 야생형 단백질과 같은, 부모 단백질의 그것과 비교하여, 알파-헬릭스 또는 베타-시트와 같은, 파괴(disrupted)되거나 또는 변화된 단백질 2차 구조를 의미한다. 변경된 2차 구조는, 예를 들어, 본원에 개시된 것과 같이, 2차 구조에서 잔기를 프롤린에 의해 치환하는 것에 의해 실현될 수 있다.
본원에 사용된, 용어 "단백질 형태(protein conformation)"는 펩타이드 체인의 2차[나선(helix), 시트, 가닥], 초이차(supersecondary) (모티프), 3차 (도메인) 및 4차(다중합체 단백질들) 구조를 포함하는, 단백질의 특징적 3차원 형상을 의미한다. 본원에 사용된, 용어 "천연 형태(native conformation)"는 생체 시스템에서 그것의 생물학적으로 활성 형태의 야생형 단백질의 특징적 상태, 형성, 모양 또는 구조를 의미한다.
본원에 사용된, 용어 "구조적 동족성(structural homology)"은 두 개 이상의 핵산들 또는 폴리펩타이드들 사이에서 공유되는 3차원 구조를 의미하고, 이것은 공유된 또는 보존된 1차 아미노산 또는 뉴클레오타이드 서열과 연관될 수 있거나 연관되지 않을 수 있다.
본원에 사용된, "변형된 3C 프로테아제(modified 3C protease)"는, 부모 피코르나바이러스 3C 프로테아제로부터 유도되거나 수득되는 피코르나바이러스 종들로부터의 전장(full-length) 3C 프로테아제를 의미한다. 본원에 사용된 "유도되거나 또는 수득된"은 부모 아미노산 서열, 또는 부모 아미노산 서열을 코딩하는 폴리뉴클레오타이드에, 하나 이상의 돌연변이, 예를 들어, 하나 이상의 아미노산들의 치환, 결실 또는 삽입, 또는, 예를 들어, 하나 이상의 아미노산의 치환, 결실, 또는 삽입을 위한 코딩하는 것을 도입하는 것을 나타낸다.
본원에 사용된, 용어 "부모(parent)" 또는 "부모 피코르나바이러스 3C 프로테아제"는, 본원의 변형된 피코르나바이러스 3C 프로테아제를 생성하기 위한 코딩 뉴클레오타이드 서열 및/또는 폴리펩타이드 서열에 돌연변이가 도입된 피코르나바이러스 3C 프로테아제를 의미한다. 상기 부모는 야생형과 상이한 야생형 피코르나바이러스 3C 프로테아제 또는 3C 프로테아제일 수 있다. 즉, 부모 3C 프로테아제는 돌연변이될 수 있어, 그것은 야생형 3C 프로테아제와 상이하나, 그럼에도 불구하고 본원에서 후속으로 변형된 폴리펩타이드와 비교하여 부모 펩타이드로서 나타낸다. 그러므로, 야생형 3C 프로테아제와 같은 변형되지 않은 기준 단백질과 비교함으로써, 특정 활성, 성질 또는 특성에서 요구되는 증가 또는 감소를 가지도록 변형된, 본 기술 분야에 공지된 존재하는 3C 프로테아제들이, 개시(starting) 폴리펩타이드로서 즉, 부모 폴리펩타이드로서 선택되어 사용될 수 있다. 예를 들어, 하나 이상의 단일 아미노산 변화에 의해 그것들의 천연 형태로부터 변형되어, 야생형 3C 프로테아제와 같은 변형되지 않은 기준 단백질과 비교하여 P1 전구체 폴리펩타이드를 가공하는 그것의 능력에서 증가 또는 감소와 같은, 요구되는 특성에서 증가 또는 감소를 보유하는 3C 프로테아제는, 부모로서 사용될 수 있고, 동일하거나 상이한 특성을 갖는, 본원의 변형된 3C 프로테아제를 수득하기 위해 추가 변형될 수 있다.
본원에 사용된, 용어 "돌연변이(mutation)"는 변경된 핵산 또는 변경된 폴리펩타이드를 생성하는 핵산 및/또는 폴리펩타이드의 임의의 유전적 변형을 나타낸다. 돌연변이들은 유전자의 단백질-코딩 영역 내에 발생하는 변경뿐만 아니라 조절 서열 또는 프로모터 서열과 같은 단백질-코딩 서열의 외부 영역에서의 변경을 포함하는, 폴리뉴클레오타이드에서 단일 또는 다중 뉴클레오타이드의 점 돌연변이, 결실, 및/또는 삽입을 포함하나, 이에 제한되는 것은 아니다. 돌연변이들은 사일런트(silent), 프레임-이동 또는 논센스 돌연변이(nonsense mutation)를 나타낼 수 있다.
용어들 "돌연변이된(mutated)", "돌연변이(mutant)", "변형된(modified)", "변경된(altered)", "변이된(variant)", 또는 "조작된(engineered)"은, 부모 3C 프로테아제와 비교하여 변경된, 피코르나바이러스 3C 프로테아제와 같은, 뉴클레오타이드 서열 또는 단백질을 기재하는 형용사로서 본원에서 서로 교환가능하게 사용된다. 비제한적인 예에 있어서, "피코르나바이러스 3C 프로테아제를 코딩하는 돌연변이된 뉴클레오타이드 서열"은, 피코르나바이러스 3C 프로테아제를 위해 코딩하는 야생형, 뉴클레오타이드 서열 코딩과 같은 부모와 상이하게 변형된 피코르나바이러스 3C 프로테아제를 위해 코딩하는 뉴클레오타이드 서열 코딩을 의미한다. 상기 돌연변이된 뉴클레오타이드 서열은 하기 중에서 적어도 하나를 야기하거나 야기하지 않을 수 있다: 하나 이상의 아미노산 치환들, 및 적절하게 접히는 (및 기능적 또는 기능적이지 않을 수 있음) 변역된 펩타이드 생성물에 대한 개방 판독 프레임에서 이동(shift). 다른 비제한적인 예에 있어서, "돌연변이된 피코르나바이러스 3C 프로테아제", "돌연변이 피코르나바이러스 3C 프로테아제", "변형된 피코르나바이러스 3C 프로테아제" 또는 "변경된 피코르나바이러스 3C 프로테아제"는, 피코르나바이러스 3C 프로테아제를 위해 코딩하는 돌연변이된 뉴클레오타이드 서열 코딩으로부터 발현되는 피코르나바이러스 3C 프로테아제를 의미하고, 상기 야생형 피코르나바이러스 3C 프로테아제와 비교하여, 예를 들어, 하나 이상의 아미노산 치환들 또는 상기 프로테아제의 부분의 결실(일반적으로 상기 C-말단으로부터)에 의하여 상기 아미노산 서열이 변경되며, 이것은, 단백질 발현 수준 (예: 형질전환 유전자 발현), 기질 특이성, 피코르나바이러스 폴리펩타이드 전구체들에 대한 숙주 단백질에 대한 단백질 분해 활성, 열적 안정성, 용해 등을 포함하는 단백질/프로테아제 특성들 중 하나 이상의 변경을 또한 유도할 수 있으나, 이에 제한되는 것은 아니다.
돌연변이, 변이된 또는 변형된 폴리펩타이드는, 본원 및 서열 목록에 기재된 것들과 같이, 공지된 피코르나바이러스 폴리뉴클레오타이드 또는 폴리펩타이드 서열과 75, 80, 85, 90, 95, 97.5, 98, 99 또는 100% 서열 상동성 또는 서열 유사성을 가질 수 있다.
BLASTN는 기준 폴리뉴클레오타이드와 적어도 75%, 80%, 85%, 87.5%, 90%, 92.5%, 95%, 97.5%, 98%, 99% 서열 상동성을 갖는 폴리뉴클레오타이드 서열을 동정하는데 사용될 수 있다. 높게 유사한 서열들을 찾기 위해 최적화된 대표적인 BLASTN 설정은 예상 임계값 10, 글자 크기 28, 0의 쿼리 범위(query range)의 최대 매치, 1/-2의 매치/미스매치 스코어, 및 선형 갭 코스트(linear gap cost)를 사용한다. 낮은 복잡도 영역은 필터링되거나 마스크될 수 있다. 표준 뉴클레오타이드 BLAST의 기본 설정은 blast.ncbi.nlm.nih.gov/_Blast.cgi?PROGRAM=blastn&BLAST_PROGRAMS=megaBlast&PAGE_TYPE=BlastSearch&SHOW_DEFAULTS=on&LINK_LOC=blasthome (2016.02.04에 마지막으로 액세스됨)에 의해 기술되며 본원에 참조로서 포함된다.
BLASTP는 기준 아미노산에 적어도 75%, 80%, 85%, 87.5%, 90%, 92.5%, 95%, 97.5%, 98%, 99% 서열 상동성 또는 유사성을 갖는 아미노산 서열을 BLOSUM45, BLOSUM62 또는 BLOSUM80과 같은 유사성 매트릭스를 사용하여 동정하기 위해 사용될 수 있으며, 여기서 BLOSUM45는 밀접한 관련 서열에, BLOSUM62는 중간 범위 서열, 및 BLOSUM80은 더 멀리 연관된 서열에 대해 사용될 수 있다. 달리 명시하지 않는 한 언급된 유사성 스코어(score)는 BLOSUM62의 사용을 기준으로 한다. BLASTP가 사용될 때, 백분율 유사성은 상기 BLASTP 양성 스코어(positives score)에 기초하고 상기 백분율 서열 상동성은 상기 BLASTP 상동성 스코어에 기초한다. BLASTP "상동성"은 상동성인 높은 스코어링 서열 쌍의 총 잔기의 수 및 비율을 나타내고; BLASTP "양성(Positives)"은 정렬 스코어가 양수 값을 가지고 서로 유사한 잔기의 수 및 비율을 나타낸다. 본원에 개시된 상기 아미노산 서열과 이러한 상동성 또는 유사성의 정도 또는 임의의 중간 정도의 상동성 또는 유사성을 갖는 아미노산 서열은 본원에 포함되고 고려된다. 대표적인 BLASTP 설정은 예상 임계값 10, 단어 크기 3, 매트릭스 BLOSUM 62, 및 갭 페널티 11 (존재) 및 1 (확장), 및 조건부 구성 스코어 매트릭스 조정을 사용한다. BLASTP에 대한 다른 기본 설정은 blast.ncbi.nlm.nih.gov/_Blast.cgi?PROGRAM=blastp&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome(2016.06.29에 마지막으로 엑세스됨)에 의해 표시되며 본원에 참조로서 포함된다.
본원에 사용된, 용어 "돌연변이체(mutant)"는 본원에서 문맥에 따라 명사로서 사용되거나, 또는 용어 "변이체(variant)"는 하기를 포함한다: 피코르나바이러스 3C 프로테아제를 코딩하는 돌연변이체 뉴클레오타이드 서열, 돌연변이체 피코르나바이러스 3C 프로테아제, 피코르나바이러스 3C 프로테아제를 코딩하는 돌연변이체 뉴클레오타이드 서열을 포함하는 형질전환 유전자 발현 카세트, 및 피코르나바이러스 3C 프로테아제를 코딩하는 돌연변이체 뉴클레오타이드 서열을 담지하는 벡터. 비제한적인 예에서, "C163A 돌연변이체", 또는 "3C(163A) 돌연변이체"는, 문맥에 따라서, 하기 중 하나를 의미한다: 위치 163에서 시스테인 잔기가 알라닌으로 치환된, FMDV 3C 프로테아제와 같은, 피코르나바이러스 3C 프로테아제를 코딩하는 뉴클레오타이드 서열, 위치 163에서 시스테인 잔기가 알라닌으로 치환된, FMDV 3C 프로테아제와 같은, 피코르나바이러스 3C 프로테아제, 위치 163에서 시스테인 잔기가 알라닌으로 치환된 FMDV 3C 프로테아제와 같은, 피코르나바이러스 3C 프로테아제를 코딩하는 뉴클레오타이드 서열을 포함하는 형질전환 유전자 발현 카세트, 및 위치 163에서 시스테인 잔기가 알라닌으로 치환된 FMDV 3C 프로테아제와 같은, 피코르나바이러스 3C 프로테아제를 코딩하는 뉴클레오타이드 서열을 담지하는 벡터.
본원에 사용된, "치환된", 또는 "치환들"은 다른 아미노산 또는 상기 부모에 존재하지 않는 아미노산을 코딩하는 코돈으로 부모 서열 (야생형 폴리펩타이드 또는 야생형 폴리뉴클레오타이드 서열)의 특정 위치에서 하나 이상의 아미노산 잔기(들) 또는 뉴클레오타이드(들)을 치환하는 것을 의미한다. 예를 들어, 상기 치환 V124P는 부모 3C 프로테아제 내에 위치 124에서 발린이 프롤린으로 치환된 변형된 폴리펩타이드를 의미한다.
본원에 사용된, 용어 "대조군 피코르나바이러스 3C 프로테아제" 또는 "대조군 3C 프로테아제"는, 본원에서 변형된 피코르나바이러스 3C 프로테아제와 비교되는, 활성, 특성, 전사 수준 및/또는 번역 수준을 갖는 피코르나바이러스 3C 프로테아제를 의미한다. 대조군 피코르나바이러스 3C 프로테아제는, 본원에 기재된 것과 같이, 야생형 FMDV 3C 프로테아제의 위치 16-25, 99-100 및 115-130에 상응하는 위치에 위치되는 하나 이상의 아미노산 치환들을 포함하지 않는 것을 제외하고 변형된 피코르나바이러스 3C 프로테아제와 동일한 1차 아미노산 서열을 갖는다. 일부 구현예들에 있어서, 대조군 피코르나바이러스 3C 프로테아제는, 야생형 3C 프로테아제와 같은, 부모 피코르나바이러스 3C 프로테아제이다. 다른 구현예들에 있어서, 대조군 피코르나바이러스는 야생형 FMDV 3C 프로테아제의 위치 20-25, 99-100 및 115-128에 상응하는 위치에 위치된 하나 이상의 치환들을 포함하지 않으나, 다른 돌연변이들을 포함한다. 예를 들어, 대조군 3C 프로테아제는 C163A와 같은, 상기 촉매성 트리아드에서 돌연변이를 제외하고, 야생형 FMDV 3C 프로테아제와 동일한 1차 서열을 가질 수 있다.
용어 "야생형(wild-type)", 그것의 약어 "wt", 및 용어 "천연(native)"은 유전적으로 변형되지 않은 생물학적 분자, 예를 들어, 자연에 존재하고 유전적으로 변형되지 않은 피코르나바이러스 3C 프로테아제를 코딩하는 뉴클레오타이드 서열, 자연에 존재하고 유전적으로 변형되지 않은 코딩 뉴클레오타이드 서열로부터 번역된 피코르나바이러스 3C 프로테아제, 자연에 존재하고 유전적으로 변형되지 않은 피코르나바이러스 3C 프로테아제를 코딩하는 뉴클레오타이드 서열을 포함하는 형질전환 유전자 발현 카세트, 및 자연에 존재하고 유전적으로 변형되지 않은 피코르나바이러스 3C 프로테아제를 코딩하는 뉴클레오타이드 서열을 담지하는 벡터를 의미한다.
본원에 사용된, "P1 전구체", "P1 전구체 폴리펩타이드" 또는 "P1 전구체 단백질"은 구조 단백질, VP0, VP1, VP2, VP3, 및 VP4 뿐만 아니라 2A 번역 인터럽터의 구조 단백질 및/또는 전구체를 포함하는 폴리펩타이드이다. P1 전구체들은, 본원에 개시된 것과 같이, 상기 피코르나바이러스 속, 종들, 혈청형 또는 균주의 임의의 것으로부터 유도될 수 있거나 또는 수득될 수 있으며, 예를 들어, P1 전구체는, 야생형 FMDV로부터 수득될 수 있거나 유도될 수 있고, 분자량이 약 85 kDa이다.
본원에 사용된, 문구 "피코르나바이러스 P1 폴리펩타이드에 대한 단백질 분해 활성을 나타낸다"는, 3C 프로테아제에 의하여, P1 전구체 폴리펩타이드를, 예를 들어, VP0, VP1 및 VP3와 같은 구조 단백질로 가공하는 것을 의미한다. 전형적으로, 상기 구조 단백질들은 비-외피형 정이십면체 (icosahedral) 캡시드 및/또는 바이러스-유사 입자들 "VLPs"로 자기조립된다.
본원에 사용된, "바이러스-유사 입자들(Virus-Like Particles)" 또는 "VLPs"는 전형적으로 바이러스 외피 또는 바이러스 캡시드로 조립되는 구조체를 나타내며, 바이러스와 유사하나, 바이러스 유전체 물질이 결여되어 비-감염성이다. VLPs 는 전형적으로 전바이러스(full virus)에 유사한 면역 반응 자극을 가능하게 한다.
본원에 사용된, 용어 "VP0 단백질"은 VP2 및 VP4 구조 단백질에 포함된 전구체 펩타이드를 의미한다. 상기 VP0 단백질은 피코르나바이러스 3C 프로테아제에 의한 상기 P1 전구체 단백질의 가공에 의해 생성된다. 일부 구현예들에 있어서, VP3 및 VP1와 함께 상기 VP0 단백질은 캡시드 및/또는 VLP 조립체에 사용될 수 있다.
본원에 사용된, "2A"는 FMDV 번역 인터럽터 서열이다 [Luke, 등, Biotech. Genetic Eng. Revs. 26: 223-260 (2009) 참조; 그 전문이 본원에 참조로서 포함됨]. 2A 폴리뉴클레오타이드 서열은 서열 번호 645의 뉴클레오타이드 34-87 및 그에 의해 코딩되는 아미노산 잔기들에 의해 기재된다. 다른 2A 서열은 서열 번호 647로 기재된 아미노산 모티프에 따를 수 있다. 다른 아프토바이러스(Apthoviruses)로부터의 2A 인터럽터들이 또한 사용될 수 있다.
본원에 사용된, 용어 "Δ1D2A"는 전형적으로 FMDV 1D 잔기들 및 FMDV 2A 아미노산 잔기들을 포함하는 번역 종료 서열을 의미한다. 서열 번호 645의 폴리뉴클레오타이드 서열은 서열 번호 646의 상기 Δ1D2A 폴리펩타이드를 코딩한다. 서열 번호 646의 상기 폴리펩타이드를 코딩하는 다른 퇴화한(degenerate) 서열들 또한 사용될 수 있다. 서열 번호 645 및 646로 나타낸 것과 같이, 폴리뉴클레오타이드 1-33은 FMDV 1D 잔기들을 코딩하고, 폴리뉴클레오타이드 34-84은 상기 2A 아미노산 잔기들을 코딩하고, 폴리뉴클레오타이드 88-90은 C-말단 프롤린 잔기를 코딩한다. Δ1D2A와 유사한 다른 번역 종료 서열들은, Δ1D2A보다 상기 1D 단백질의 잔기가 더 적거나 많을 수 있고, 또는 번역 종료 서열로서 작용하는 그것의 능력에 영향을 미치지 않는 상기 2A 서열에 1, 2, 3 또는 그 이상의 점 돌연변이들을 포함할 수 있다.
본원에 사용된, "가우시아 루시퍼라아제 (Gaussia luciferase)"(GLuc)는 작고, 자연적으로 분비된 185 개 아미노산 (서열 번호 649) 루시퍼라아제 및 상기 분비된 루시퍼라아제를 코딩하는 핵산을 의미한다. 상기 용어는, 예를 들어, 서열 번호 649로 나타내는, 천연 GLuc 단백질과 적어도 90%, 적어도 95%와 같은 또는 적어도 99%와 같은 서열 상동성을 갖는 단백질들 및 루시퍼라아제 특성들을 나타내는 GLuc 변이체를 코딩하는 핵산들을 포함하는 것을 또한 의미한다. 전형적으로, GLuc은 반딧불 또는 레닐라 (Renilla) 루시퍼라아제와 비교할 때 더 높은 강도를 가진다.
본원에 사용된, "SGLuc"는 저수준의 단백질 발현을 조사하는데 유용한 초발광성 GLuc 변이체 (예: 서열 번호 651)를 의미한다. SGLuc 변이체는, 전형적으로 GLuc 루시퍼라아제의 분비형을 발현 및/또는 분비될 수 있는 GLuc 루시퍼라아제를 발현하는 가우시아 루시퍼라아제 (Gaussia luciferase) (GLuc) 유전자의 아미노산 89 및 90에서 돌연변이를 포함한다.
본원에서 사용된, "벡터(vector)"는 유기체, 세포, 또는 세포 성분들 사이에서 핵산이 전파되고/되거나 전달될 수 있는 임의의 수단을 나타낸다. 벡터는 바이러스, 박테리오파지, 프로-바이러스, 플라스미드, 파지미드(phagemids), 트랜스포존(transposon), 코즈미드(cosmide), 바이러스 벡터, 발현 벡터, 유전자 전달 벡터, 미니서클 벡터, 및 효모 인공 염색체 (yeast artificial chromosomes, YACs), 박테리아 인공 염색체 (bacterial artificial chromosomes, BACs), 및 식물 인공 염색체 (plant artificial chromosomes, PLACs) 등과 같은 인공 염색체를 포함하는 것으로서, "에피좀", 즉, 자율적으로 복제하거나 숙주 세포의 염색체에 집적될 수 있는 것이다. 벡터는 전형적으로 적어도 복제의 원점, 복제 부위 및 선택가능한 마커 (예: 항생제 내성)를 포함한다. 전술한 비제한적인 예들의 천연 버전은 단리, 정제, 및/또는 변형될 수 있어, 그 수득되는 천연 버전은 그것의 자연 상태의 물질과 구별될 수 있다. 벡터는 또한 네이키드(naked) RNA 폴리뉴클레오타이드, 네이키드 DNA 폴리뉴클레오타이드, 동일한 가닥 내에 DNA 및 RNA 모두로 구성된 폴리뉴클레오타이드, 폴리라이신-컨쥬게이트된 DNA 또는 RNA, 펩타이드-컨쥬게이트된 DNA 또는 RNA, 리포좀-컨쥬게이트된 DNA 등으로서, 본질적으로 에피좀이 아니거나, 또는 아그로박테리움(Agrobacterium) 또는 박테리아와 같은 하나 이상의 상기 폴리뉴클레오타이드 구조체를 포함하는 유기체일 수 있다.
본원에 사용된 용어 "재조합 벡터"는 상이한 소스로부터 핵산 조각들을 연결함으로써 생성된 벡터로 정의된다.
"미니서클 DNA 벡터 (minicircle DNA vector)", "미니서클 벡터", 또는 "미니서클"은 작은 (일반적으로 3 kb 내지 4kb, 또는 일반적으로 10kb 이하) 원형, 에피좀 플라스미드 유도체로서, 모든 원핵생물 벡터 부분 (예: 박테리아 복제 원점, 플라스미드의 박테리아 전파와 연관된 유전자)이 제거된 것이다. 미니서클 벡터는 원핵생물 DNA 서열을 포함하지 않기 때문에, 그것들이 목적 포유동물 세포로 형질전환 유전자를 전달하기 위한 비히클로서 사용될 때 외래종으로 인식될 가능성이 적고, 파괴될 가능성이 적다.
"형질전환 유전자 발현 카세트", "형질전환 유전자 발현 구조체", "발현 카세트", "발현 구조체", "구조체(construct)", "키메라(chimera)", "키메라성 DNA", "DNA 키메라" 또는 "키메라성 유전자"는 자연에서 함께 발견되지 않는 하나 이상의 기능적 단위들 [예: 프로모터, 조절 요소, 컨센서스 서열(consensus sequence), 번역 프레임-이동 서열, 단백질 코딩 유전자 등]을 포함하도록 인공적으로 구축된 핵산 서열이며, 목적 서열(들)을 코딩하는 유전자 뿐만 아니라 shRNAs, microRNAs, siRNAs, 안티-센스 RNAs와 같은 비-번역된 RNAs를 포함하여, 상기 카세트가 전달된 유기체에서 임의의 RNA 전사의 발현을 지시할 수 있다, 형질전환 유전자 발현 카세트는 단일- 또는 이중-가닥이고 원형 또는 선형일 수 있다. 형질전환 유전자 발현 카세트는 표적 세포에서 핵산 서열을 전달, 복제 및/또는 발현시키기 위한 비히클로서 작용하는 것으로서, 벡터에 구축되거나, 삽입 또는 복제될 수 있다.
본원에 사용된, 용어 "형질전환"은 벡터 또는 폴리뉴클레오타이드 구조체가 숙주 세포에 도입되는 과정을 나타낸다. 형질전환 [또는 형질도입 (transduction), 또는 형질주입 (transfection)]은 화학적 형질전환 (예, 아세트산 리튬 형질전환), 전기천공 (electroporation), 미세주입(microinjection), 바이오리스틱 (biolistic) [또는 입자 폭격 (particle bombardment)-매개 전달] 또는 아그로박테리움 매개 형질전환을 포함하는 다수의 수단들 중 임의의 하나에 의해 달성될 수 있다.
본원에 사용된, 용어 "형질주입"은 벡터 (DNA 또는 RNA) 내에서 복제된 유전자와 같은 핵산이 진핵생물 숙주 세포 내로 전달되는 과정을 나타낸다.
본원에 사용된, 용어 "숙주 세포"는, 자연적으로 감염되거나, 예를 들어, 백신접종에 의하여, 바이러스 또는 벡터로 인공적으로 형질주입되거나 형질전환된 원핵생물 세포 (예: 박테리아) 또는 진핵생물 세포 (예: 포유동물, 곤충, 효모 등) 를 의미한다. 상기 숙주 세포에 도입된 상기 바이러스는 살아있거나(live), 불활성화, 약화 또는 변형될 수 있지만, 상기 도입된 벡터는, 숙주 세포에서 발현될 때, 자기조립하여 바이러스-유사 입자(VLPs)를 형성하는 바이러스 구조 단백질을 생성할 수 있는, 형질전환 유전자 발현 카세트를 담지한다. 일부 경우, 숙주 세포는 숙주 또는 대상의 내부에 있을 수 있고, 상기 숙주 또는 대상은, 변형된 FMDV 3C 프로테아제와 같은, 본원의 변형된 피코르나바이러스 3C 프로테아제, 및 FMDV P1 전구체와 같은 적어도 하나의 다른 피코르나바이러스 항원을 코딩하는 핵산-기반 백신의 투여에 의해 치료될 수 있다. 일부 구현예들에 있어서, 변형된 피코르나바이러스 3C 프로테아제-코딩 폴리뉴클레오타이드는 본 기술분야에 잘 공지된 트랜스포존의 사용에 의한, 또는 다른 재조합 DNA 방법들에 의한, 재조합을 통해 숙주 세포 게놈에 도입된다. FMDV 3C 프로테아제와 같은 피코르나바이러스 3C 프로테아제, 및 FMDV P1 전구체와 같은 다른 피코르나바이러스 항원들은 숙주 세포 내부의 동일하거나 상이한 플라스미드, 에피좀 및/또는 다른 DNA 또는 RNA 구조체들로부터 발현될 수 있다.
피코르나바이러스 3C 프로테아제, 피코르나바이러스 P1 전구체 단백질, 다른 단백질들 또는 항원 서열뿐만 아니라, 다른 목적 단백질의 발현을 위한 숙주 세포는 원핵생물 또는 진핵생물 세포일 수 있다. 상기 용어 "숙주 세포"는 사카로미세스 세레비시아 (Saccharomyces cerevisiae), 또는 피히아 파스토리스 (Pichia pastoris)와 같은, 효모 또는 곰팡이 숙주 세포; 애기장대 (Arabidopsis thaliana), 클라미도모나스 레인하티 (Chlamydomonas reinhardtii), 대두 (Glycine max), 니코티아나 벤타미아나 (Nicotiana benthamiana), 재배담배 (Nicotiana tabacum), 벼 (Oryza sativa), 옥수수 (Zea mays)와 같은, 식물 숙주 세포; 또는 담배거세미나방종 (Spodoptera frugiperda), 노랑초파리 (Drosophila melanogaster), Sf9, Sf21과 같은 곤충 세포 또는 곤충 세포주; HEK-293-T (인간 신장 배아) 세포, 중국 햄스터 난소 [Chinese Hamster Ovary (CHO)] 세포, 아기 햄스터 신장(BHK) 세포, LF-BK (돼지 세포), LF-BK αV/β6, 또는 피코르나바이러스 감염에 민감한 동물과 같은 척추 동물의 세포 또는 포유동물의 세포 또는 포유동물 세포주; 간균 (Bacillus), 락토코코스 (Lactococcus), 스트렙토미세스 (Streptomyces), 로도코커스 (Rhodococcus), 코리네박테리움 (Corynebacterium), 미코박테륨 (Mycobacterium)과 같은 그람-양성 박테리아와 같은 원핵생물 숙주 세포 또는 대장균 (Escherichia), 슈도모나스(Pseudomonas)와 같은 그람-음성 박테리아와 같은 원핵생물 숙주 세포를 포함한다.
본원에 사용된, 용어 "잔기(residue)" 또는 "아미노산 잔기"는 펩타이드, 폴리펩타이드 또는 단백질의 폴리머 체인 내에 특정 아미노산을 의미한다. 상기 용어는 22 개의 통상적인 단백질 생성 아미노산 잔기 (셀레노시스테인 및 피롤라이신을 포함), 변형된 단백질 생성 아미노산 잔기, 및/또는 비-단백질 생성 아미노산 잔기 중 임의의 것을 포함한다. 본원을 통해, 아미노산 잔기는 3 개의 글자 코드 또는 단일 글자 코드로 나타낼 수 있으며, 알라닌의 경우 Ala (A), 아르기닌의 경우 Arg (R), 아스파라긴의 경우 Asn (N), 아스파라긴 산의 경우 Asp (D), 시스테인의 경우 Cys (C), 글루타민의 경우 Gln (Q), 글루타민 산의 경우 Glu (E), 글리신의 경우 Gly (G), 히스티딘의 경우 His (H), 이소류신의 경우 Ile (I), 류신의 경우 Leu (L), 라이신의 경우 Lys (K), 메티오닌의 경우 Met (M), 페닐알라닌의 경우 Phe (F), 프롤린의 경우 Pro (P), 세린의 경우 Ser (S), 트레오닌의 경우 Thr (T), 트립토판의 경우 Trp (W), 티로신의 경우 Tyr (Y), 발린의 경우 Val (V), 피롤라이신의 경우 Pyl (O), 셀레노시스테인의 경우 Sec (U)를 포함할 수 있으나, 이에 제한되는 것은 아니다.
용어 "아미노산 서열", "펩타이드 서열" 또는 "단백질 서열"은, 펩타이드 결합들에 의해 연결된 아미노산 잔기들이 펩타이드 또는 단백질 체인에서 나타나는 순서를 의미한다. 아미노산 서열은 일반적으로 유리(free) 아미노기를 포함하는 N-말단으로부터 유리 카르복실기를 포함하는 상기 C-말단으로 리포트(report)된다. 본원에 사용된, "비-코딩된 아미노산", "비-단백질 생성 아미노산", "합성 아미노산" 또는 "비천연 아미노산"은 임의의 유기체의 유전자 코드 (DNA 또는 mRNA)로 자연적으로 코딩되지 않거나 자연적으로 발견되지 않아, in vitro 합성되어야 하는 아미노산을 나타낸다.
"유전적으로 코딩된 아미노산", "코딩된 아미노산" 또는 "천연 아미노산"은, 알라닌, 아르기닌, 아스파라긴, 아스파라긴산, 시스테인, 글루타민, 글루타민산, 글리신, 히스티딘, 이소류신, 류신, 라이신, 메티오닌, 페닐알라닌, 프롤린, 세린, 트레오닌, 트립토판, 티로신, 발린, 피롤라이신 및 셀레노시스테인과 같은, 유기체의 유전자 코드 (DNA 또는 mRNA)로 자연적으로 코딩되거나 상기 유전자 코드에서 발견되는 아미노산을 의미한다.
본원에서 사용된, 용어 "예방하다(prevent)" 및 "예방하는(preventing)"은 질병, 장애, 상태 및/또는, 피코르나바이러스 감염과 같은, 감염의 재발, 전염 또는 발병의 예방을 포함한다. 본원의 개시가 완전한 예방으로 제한되는 것은 아니다. 본원의 일부 구현예들에 있어서, 예방은 질병 발병을 지연시키거나, 중증을 감소시키거나, 전염을 감소시키거나, 또는 증상 및 예후(presentation)를 변경시킨다.
본원에서 사용된, 용어 "치료하다(treat)" 및 "치료하는(treating)"는 질병 진행 지연, 특정 증상의 감소, 전염의 감소를 포함하고, 또는 질병 예후, 증상 또는 진행에 영향을 미친다. 본원에 따른 백신을 이용한 예방 또는 치료는 세포 면역 (예: T 세포를 통한) 또는 체액(humoral) 면역 (예: 항체를 통한)의 유도를 포함할 수 있다. 이러한 백신은, 변형된 FMDV 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 발현하는 숙주 세포에 의해 생성된, 하나 이상의 FMDV 항원과 같은, 하나 이상의 피코르나바이러스 항원을 일반적으로 포함할 것이다. 그러나, 변형된 FMDV 3C 프로테아제와 같은 변형된 피코르나바이러스 3C 프로테아제, 및, 다른 FMDV 항원(들)과 같은 다른 피코르나바이러스 항원들을 발현하는 DNA-기반 백신 또한 고려된다.
피코르나바이러스 VLPs의 생성, 유전자 발현 (예: FMDV 폴리펩타이드 전구체, 야생형 또는 변형된 FMDV 3C 프로테아제의 발현 등), DNA 전사, mRNA 번역, FMDV과 같은 피코르나바이러스의 절단, 폴리펩타이드 전구체 (예: P1 등)의 생성과 같은 반응을 나타낼 때, 용어 "in vivo"는 바이러스 숙주 세포와 같은, 살아있는 세포의 환경 내에서 상기 반응이 일어남을 의미한다. 상기 살아있는 세포는 숙주 또는 다른 유기체 또는 인공 배양 배지 내에서 살아있는 세포일 수 있다.
VLPs, 유전자 발현 (예: FMDV 폴리펩타이드 전구체, 야생형 또는 변형된 FMDV 3C 프로테아제의 발현 등), DNA 전사, mRNA 번역, FMDV과 같은, 피코르나바이러스의 절단, 폴리펩타이드 전구체 (예: P1 등)의 생성과 같은 반응을 나타낼 때, 용어 "in vitro"는 시험관, 플라스크, 페트리 접시 등에서 용액, 액체/고체 배양 배지를 포함하는, 살아있는 세포를 제외한 임의의 환경에서 상기 반응이 일어남을 의미한다.
[자세한 설명]
FMDV 3C 프로테아제 내 아미노산 위치에 상응하는 위치에서 변형을 갖는 폴리뉴클레오타이드 및 폴리펩타이드
FMDV 상응 위치
본원의 일 측면은 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이다. 일부 구현예들에 있어서, 상기 단리된 폴리뉴클레오타이드에 의해 코딩되는 변형된 피코르나바이러스 3C 프로테아제는 아미노산 서열 번호 2, 4, 6, 10, 12, 14, 16, 18 및 20으로 표시되는 야생형 FMDV 3C 프로테아제 서열과 같은, 야생형 FMDV 3C 프로테아제의 아미노산 위치(들) 16-25, 99-100 및 115-130에 상응하는 위치(들)에 위치하는 하나 이상의 아미노산 치환을 포함한다. 이러한 단리된 폴리뉴클레오타이드들에 의해 코딩된 변형된 3C 프로테아제들 또한 고려된다.
다른 피코르나바이러스 상응 위치
일부 측면들에 있어서, 본원은 또한 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이며, 상기 변형된 피코르나바이러스 3C 프로테아제는, 예를 들어, 야생형 소 비염(Bovine rhinitis) 3C 프로테아제의 아미노산 위치 22, 23, 96, 97, 121, 123 및 124 중 하나 이상에 상응하는 위치에 위치하는 하나 이상의 아미노산 치환들을 포함하는 변형된 소 비염(Bovine rhinitis) 3C 프로테아제 치환들; 야생형 말 비염(Equine rhinitis) 3C 프로테아제의 아미노산 위치 23, 100, 125 및 127 중 하나 이상에 상응하는 위치에 위치하는 하나 이상의 아미노산 치환들을 포함하는 변형된 말 비염(Equine rhinitis) 3C 프로테아제; 야생형 인간 콕사키바이러스(Human coxsackievirus)의 19, 20, 87, 102, 104, 105, 106, 109, 112 및 114 아미노산 위치 중 하나 이상에 상응하는 위치에 위치하는 하나 이상의 아미노산 치환들을 포함하는 변형된 인간 콕사키바이러스 3C 프로테아제; 야생형 인간 엔테로바이러스 3C 프로테아제의 아미노산 19, 20, 104, 105, 106 및 109 중 하나 이상에 상응하는 위치에 위치하는 하나 이상의 아미노산 치환들을 포함하는 인간 엔테로바이러스 3C 프로테아제; 야생형 인간 폴리오바이러스(poliovirus) 3C 프로테아제의 아미노산 19, 20, 100, 119, 121, 128 및 130 중 하나 이상에 상응하는 위치에 위치하는 하나 이상의 아미노산 치환들을 포함하는 인간 폴리오바이러스 3C 프로테아제; 및/또는 야생형 인간 리노바이러스 (Human rhinovirus) 3C 프로테아제의 아미노산 위치 19, 20, 87, 88, 102, 104, 108, 112 및 114 중 하나 이상에 상응하는 위치에 위치하는 하나 이상의 아미노산 치환들을 포함하는 인간 리노바이러스 3C 프로테아제; 야생형 인간 간염 바이러스(Human hepatitis) 3C 프로테아제의 아미노산 19, 20, 100, 119, 121, 128 및 130 중 하나 이상에 상응하는 위치에 위치하는 하나 이상의 아미노산 치환들을 포함하는 A 형 간염 바이러스(Hepatitis A virus) 3C 프로테아제로부터 선택된다. 이러한 단리된 폴리뉴클레오타이드들에 의해 코딩되는 변형된 3C 프로테아제 또한 고려된다.
예시적 야생형 서열
일부 구현예들에 있어서, 부모 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드 서열 또는 부모 피코르나바이러스 3C 프로테아제는 변형되어 본원의 상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제를 생성한다. 일부 구현예들에 있어서, 변형되는 부모 폴리뉴클레오타이드 또는 부모 폴리펩타이드는 본 기술 분야에 공지되고 본원에서 제공되는 임의의 피코르나바이러스 속, 종, 아형, 이디오타입(idiotype), 균주 및/또는 단리물(isolate)로부터의 것과 같은, 임의의 야생형 피코르나바이러스 3C 프로테아제로부터 수득되거나 유도될 수 있다. 예를 들어, 부모 피코르나바이러스 3C 프로테아제를 코딩하는 부모 폴리뉴클레오타이드 또는 부모 피코르나바이러스 3C 프로테아제는 아프토바이러스 (Aphthovirus), 아쿠아마바이러스 (Aquamavirus), 아비헤파토바이러스 (Avihepatovirus), 카디오바이러스 (Cardiovirus), 코사바이러스 (Cosavirus), 디시피바이러스 (Dicipivirus), 엔테로바이러스 (Enterovirus), 에르보바이러스(Erbovirus), 헤파토바이러스 (Hepatovirus), 코부바이러스 ( Kobuvirus), 메기바이러스 (Megrivirus), 파레코바이러스 (Parechovirus), 피스세바이러스 (Piscevirus), 살리바이러스 (Salivirus), 사펠로바이러스 (Sapelovirus), 세네카바이러스(Senecavirus), 테스코바이러스 (Teschovirus) 및 트레모바이러스(Tremovirus)를 포함하는 상기 피코르나바이러스 속의 임의로부터 수득되거나 유도될 수 있다. 특별히 적합한 속은 아프토바이러스 (Aphthovirus), 엔테로바이러스 (Enterovirus), 및 헤파토바이러스 (Hepatovirus)를 포함한다.
3C 프로테아제 또는 부모 3C 프로테아제를 코딩하는 부모 폴리뉴클레오타이드가 수득되거나 유도되어 본원의 상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 상기 단리된 폴리뉴클레오타이드 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제를 생성하기 위한 상기 아프토바이러스 (Aphthovirus) 속 내에 특별히 적합한 종들은 FMDV의 혈청형, 예를 들어, A, O, C, Asia 1, SAT1, SAT2, 및 SAT3 혈청형의 임의의 혈청형, 아형, 지역형, 및/또는 균주, 균주 sd-1과 같은, 소 비염 A 바이러스 (Bovine rhinitis A virus), 소 비염 B 바이러스 (Bovine rhinitis B virus) 및 말 비염 A 바이러스 (Equine rhinitis A virus)를 포함한다. 적합한 야생형 FMDV 3C 프로테아제의 특정 예는 서열 번호 2 (A Turkey 2006), 서열 번호 4 (A24 Cruzeiro iso71), 서열 번호 6 (Asia Lebanon 89, 혈청형 Asia 1), 서열 번호 8 (Asia 1 shamir), 서열 번호 10 (C3 Indaial), 서열 번호 12 (O1 PanAsia), 서열 번호 14 (O1 Manisa 분리주 87 균주, 혈청형 O), 서열 번호 16 (SAT1-20 iso11), 서열 번호 18 (SAT2 Egypt 2010) 및 서열 번호 20 (SAT3 ZIM/6/91)를 포함한다. 적합한 부모 FMDV 폴리뉴클레오타이드 3C 프로테아제 서열의 예는 각각 서열 번호 2, 4, 6, 8, 10, 12, 14, 16, 18 및 20을 코딩하는, 서열 번호 1, 3, 5, 7, 9, 11, 13, 15, 17 및 19로 기재된 것들을 포함한다. 부모 3C 프로테아제로서 사용될 수 있는 야생형 소 비염(Bovine rhinitis) 3C 프로테아제의 예는 서열 번호 422 [소 비염 A 바이러스 (Bovine rhinitis A virus)] 및 서열 번호 434 [소 비염 B 바이러스 (Bovine rhinitis B virus)]를 포함한다. 적합한 부모 소 비염(Bovine rhinitis) 폴리뉴클레오타이드 3C 프로테아제 서열의 예는 서열 번호 421 [소 비염 A 바이러스 (Bovine rhinitis A virus)] 및 서열 번호 433 [소 비염 B 바이러스 (Bovine rhinitis B virus)]로 기재된 것들을 포함한다. 부모로서 사용될 수 있는 야생형 말 비염(Equine rhinitis) 3C 프로테아제의 예는 서열 번호 444를 포함한다. 적합한 부모 말 비염(Equine rhinitis) 폴리뉴클레오타이드 3C 프로테아제 서열의 예는 서열 번호 443으로 기재된 것을 포함한다.
3C 프로테아제를 코딩하는 부모 폴리뉴클레오타이드 또는 부모 3C 프로테아제가 수득되거나 유도될 수 있는 특히 적합한 부모 엔테로바이러스(Enterovirus) 종들은 엔테로바이러스 A (Enterovirus A), 엔테로바이러스 B (Enterovirus B), 엔테로바이러스 C (Enterovirus C), 리노바이러스 A (Rhinovirus A) 및 리노바이러스 B (Rhinovirus B)를 포함한다. 적절한 엔테로바이러스 A (Enterovirus A) 혈청형 및 균주는 Kowalik 균주와 같은 인간 콕사키바이러스(Human coxsackievirus) A10, 및 인간 엔테로바이러스 (Human enterovirus) A71, C4 균주와 같은 인간 엔테로바이러스(Human enterovirus) A71을 포함한다. 적합한 엔테로바이러스 B (Enterovirus B) 혈청형 및 균주는, 인간 콕사키바이러스 (Human coxsackievirus) B1, 마코시 (Macocy) 균주와 같은 인간 콕사키바이러스 (Human coxsackievirus) B3, 및 돼지 수포 병 바이러스(swine vesicular disease virus)와 같은 인간 콕사키바이러스 B5, 예를 들어, 서열 번호 640에 기재된 것과 같은 야생형 돼지 수포 병 바이러스 3C 프로테아제와 같은 부모 3C 프로테아제를 포함한다. 적합한 엔테로바이러스 C (Enterovirus C) 혈청형, 균주 및 단리물은 Tompkins 균주와 같은 인간 콕사키바이러스 A1, Mahoney 균주와 같은 인간 폴리오바이러스 1 (poliovirus 1), 균주 R93152와 같은 인간 폴리오바이러스 2 (Human poliovirus 2), 및 단리물 CHN5275/JX/CHN/2001과 같은 인간 폴리오바이러스 3 (Human poliovirus 3)을 포함한다. 특히 적합한 리노바이러스 A (Rhinovirus A) 혈청형 및 균주는 인간 리노바이러스 (Human rhinovirus) A2, 균주 VR1130과 같은 인간 리노바이러스 (Human rhinovirus) A20, 및 균주 R1199과 같은 인간 리노바이러스 (Human rhinovirus) A89를 포함한다. 적합한 리노바이러스 B (Rhinovirus B) 혈청형은 인간 리노바이러스(Human rhinovirus) B14를 포함한다. 특히 적합한 헤파토바이러스(Hepatovirus) 종들은 인간 A 형 간염 바이러스(Human hepatitis A virus)를 포함한다.
적합한 부모 엔테로바이러스(Enterovirus) 3C 프로테아제의 특정 예는 서열 번호 478로 기재된 야생형 인간 콕사키바이러스(Human coxsackievirus) A10 3C 프로테아제, 서열 번호 480으로 기재된 야생형 인간 콕사키바이러스 B1 3C 프로테아제, 서열 번호 510으로 기재된 야생형 인간 콕사키바이러스 B3 3C 프로테아제, 서열 번호 462로 기재된 야생형 인간 콕사키바이러스 A1 3C 프로테아제 및 서열 번호 526으로 기재된 야생형 인간 엔테로바이러스(Human enterovirus) A71 3C 프로테아제를 포함한다. 부모로서 사용하기 위한 특히 적합한 인간 A 형 간염 바이러스는 서열 번호 538로 기재되어 있다.
엔테로바이러스(Enterovirus) 3C 프로테아제를 코딩하는 적합한 부모 폴리뉴클레오타이드의 예에는 서열 번호 477로 기재된 야생형 인간 콕사키바이러스 A10 3C 프로테아제, 서열 번호 479로 기재된 야생형 인간 콕사키바이러스 B1 3C 프로테아제, 서열 번호 509로 기재된 야생형 인간 콕사키바이러스 B3 3C 프로테아제, 서열 번호 461로 기재된 야생형 인간 콕사키바이러스 A1 3C 프로테아제, 서열 번호 525로 기재된 야생형 인간 엔테로바이러스 A71 3C 프로테아제 및 서열 번호 439로 기재된 것과 같은 야생형 돼지 수포 병 바이러스 3C 프로테아제를 코딩하는 것들을 포함한다. 부모로서 사용하기 위한 특히 적합한 인간 A 형 간염 바이러스는 서열 번호 537로 기재되어 있다.
변형에 적합한 부모 피코르나바이러스 3C 프로테아제를 수득하거나 유도하기 위해 사용될 수 있는 다른 적합한 피코르나바이러스는, 서열 번호 636에 기재된, 돼지 테스코바이러스 (Porcine Teschovirus) 3C 프로테아제와 같은 테스코바이러스 (Teschovirus genus) 속, 및, 서열 번호 638에 기재된 것, 세네카 밸리 (Sereca Valley) 바이러스 3C 프로테아제와 같은 세네카 바이러스(Senecavirus) 속을 포함한다. 돼지 테스코바이러스(Porcine Teschovirus) 3C 프로테아제를 코딩하는 적합한 부모 폴리뉴클레오타이드의 예는 서열 번호 635로 기재된 야생형 돼지 테스코바이러스(Porcine Teschovirus) 3C 프로테아제를 포함한다. 세네카 밸리 (Seneca Valley) 바이러스 3C 프로테아제를 코딩하는 적합한 부모 폴리뉴클레오타이드의 예는 서열 번호 637에 기재된 야생형 세네카 밸리 (Sereca Valley) 바이러스 3C 프로테아제를 포함한다.
상기 및 본원에 기재된 상기 야생형 3C 프로테아제 서열 및 상기 야생형 3C 프로테아제를 코딩하는 상기 단리된 폴리뉴클레오타이드는, 본원에 기술된 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드를 달성하기 위하여 변형되는 부모 피코르나바이러스 3C 프로테아제로서, 및/또는, 예를 들어, 야생형 FMDV 3C 프로테아제 아미노산 위치들 16-25, 99-100 및 115-130 또는 이러한 위치들을 코딩하는 코돈에 상응하는 피코르나바이러스 3C 프로테아제에서 위치를 결정하기 위한 정렬의 주형으로서 본원에 기술된 임의의 구현예들에서 사용될 수 있다.
상응 위치의 결정
야생형 FMDV 3C 프로테아제의 아미노산 위치(들) 16-25, 99-100 및 115-130에 상응하는 상기 부모 피코르나바이러스 3C 프로테아제에서 위치(들)은, 예를 들어, 비교 모델링을 사용하여 결정될 수 있다. 상기 구현예들에 있어서, 부모 3C 프로테아제의 3-차원 모델은 공지된 FMDV 3C 프로테아제의 3-차원 구조에 기초하여 1차 아미노산 서열로부터 구축될 수 있다. 모델을 구축할 수 있게 하기 위해, 상기 부모 피코르나바이러스 3C 프로테아제는, 예를 들어, FMDV 3C 프로테아제와 적어도 30% 서열 상동성을 나타내는 것이 보통 바람직하다. 상기 모델 구조는 본 기술 분야에 공지된 임의의 적합한 소프트웨어, 예를 들어, Modeller (Andrej Sali, Roberto Sanchez, Azat Badretdinov, Andrαs Fiser, and Eric Feyfant, The Rockefeller University, 1230 York Avenue, New York, N.Y. 10021-6399, USA), 또는 WHAT IF: A molecular modeling and drug design program (G. Vriend, J. Mol. Graph. (1990) 8, 52-56)를 사용하여 구축될 수 있고, 여기서 그 전문은 본원에 참조로서 포함된다. 생성된 비교 모델은 FMDV 3C 프로테아제에 대한 3차원 구조 유사성에 대해 스코어링(scored) 될 수 있다. 예를 들어, TM 정렬과 같은 프로그램, DALI 서버 (예: DaliLite v.3 available at ekhidna.biocenter.helsinki.fi/dali_server) 또는 PyMOL에서 수행되는 cealign 알고리즘(예: version 1.7.6, www.pymol.org에서 이용가능함)은 각각 TM 정렬 스코어, Z-스코어, 또는 RMSD 값을 계산함으로써 구조 유사성을 평가하기 위해 사용될 수 있다.
그러나, 더 전형적으로, 상기 상응하는 위치(들)은, 편리하게 하나 이상의 부모 피코르나바이러스 3C 프로타아제(들), 전형적으로 야생형 3C 프로타아제들로부터 수득되거나 또는 유도되는 하나 이상의 1차 아미노산 서열(들), 및 하나 이상의 야생형 FMDV 3C 프로테아제(들)와 같은, 하나 이상의 야생형 3C 프로타아제로부터 수득되거나 또는 유도되는 하나 이상의 1차 아미노산 서열(들) 사이의 정렬에 기초하여 편리하게 결정된다. 상기 1차 아미노산 서열(들)은 상기 본 기술 분야에 공지된 임의의 방법에 의해 정렬될 수 있다. 이러한 방법들은 전형적으로 매치를 최대화시키며 매뉴얼 정렬들을 사용하는 방법들을 포함하고/포함하거나 이용가능한 다수의 정렬 프로그램들[예: 기본 매개변수를 사용하는 BLASTP 및 CLUSTALW 버전 1.74 및 Thompson 등, 1994, CLUSTAL W: "Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice", Nucleic Acids Research, 22:4673-4680, 그 전문이 본원에 참조로서 포함됨]을 사용한다. 일부 구현예들에 있어서, 기본 매개변수를 갖는 Blossum 62를 사용하는 2015년 1월 1일, version 9.4, Professional Edition, Clone Manager 9는 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 3C 프로테아제를 달성하고/하거나 본원의 변형된 3C 프로테아제를 달성하기 위해 부모 3C 프로테아제에서 치환될 수 있는 상응하는 아미노산(들)을 결정하기 위한 상기 정렬을 제조하기 위해 사용된다.
상응하는 위치들의 예
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 본원의 변형된 3C 프로테아제는, 야생형 FMDV 3C 프로테아제의 아미노산 위치들 22 및 23과 같은 아미노산 위치들 16-25에 상응하는 위치(들)에 위치하는 하나 이상의 아미노산 치환들을 포함하도록 변형된 부모 피코르나바이러스 3C 프로테아제이다. 야생형 FMDV 3C 프로테아제의 위치 22 및 23에 상응하는 부모 피코르나바이러스 3C 프로테아제에서 아미노산 위치들의 예들은, 예를 들어, 야생형 FMDV 3C 프로테아제와 같은 야생형 아프토바이러스(Aphthovirus)의 I22 및 L23 각각, 야생형 소 비염 A 바이러스 (Bovine rhinitis A virus) 3C 프로테아제와 같은 야생형 아프토바이러스 (Aphthovirus)의 V22 및 C23 각각, 야생형 말 비염 B 바이러스 (Equine rhinitis B virus) 3C 프로테아제와 같은 야생형 아프토바이러스 (Aphthovirus) 3C 프로테아제의 V22 및 R23 각각, 및, 야생형 말 비염 A 바이러스 (Equine rhinitis A virus) 3C 프로테아제와 같은 야생형 아프토바이러스 (Aphthovirus) 3C 프로테아제의 Y22 및 C23 각각을 포함한다. 야생형 FMDV 3C 프로테아제, 소 비염 A 바이러스 (Bovine rhinitis A virus) 3C 프로테아제, 소 비염 B 바이러스 (Bovine rhinitis B virus) 3C 프로테아제 및 말 비염 A 바이러스 (Equine rhinitis A virus) 3C 프로테아제 사이의 정렬을 나타내는 도 4 참조.
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 3C 프로테아제는 야생형 FMDV 3C 프로테아제의 아미노산 위치들 20 및 21과 같은, 아미노산 위치들 16-25 상응하는 위치(들)에 위치하는 하나 이상의 아미노산 치환들을 포함하는 피코르나바이러스 3C 프로테아제이다. 야생형 FMDV 3C 프로테아제의 위치들 20 및 21에 상응하는 부모 피코르나바이러스 3C 프로테아제에서 아미노산 위치들의 예들은, 예를 들어, 야생형 인간 콕사키바이러스 A10 3C 프로테아제와 같은 야생형 엔테로바이러스 A 3C 프로테아제의 Q19 및 T20 각각, 및 야생형 인간 엔테로바이러스 A71 3C 프로테아제와 같은, 야생형 엔테로바이러스 A 3C 프로테아제의 Q19 및 T20 각각을 포함한다 (도 5 및 도 6 참조).
다른 구현예들에 있어서, 야생형 FMDV 3C 프로테아제의 아미노산 위치들 20 및 21에 상응하는 위치들은, 예를 들어, 야생형 인간 콕사키바이러스 B1 또는 B3 프로테아제과 같은, 야생형 엔테로바이러스 B (Enterovirus B) 3C 프로테아제의 K19 및 T20 각각을 포함한다 (도 7 참조).
야생형 FMDV 3C 프로테아제의 위치들 21 및 22에 상응하는 부모 피코르나바이러스 3C 프로테아제에서 아미노산 위치들의 예들은, 예를 들어, 야생형 인간 콕사키바이러스 A1 3C 프로테아제 및 야생형 인간 폴리오바이러스 3C 프로테아제와 같은 야생형 엔테로바이러스 C 3C 프로테아제의 T19 및 T20 각각을 추가 포함한다 (도 5 및 도 8 참조).
다른 구현예들에 있어서, 야생형 FMDV 3C의 아미노산 위치들 21 및 22에 상응하는 위치들은, 예를 들어, 야생형 리노바이러스 A 3C 프로테아제의 T19 및 T20 각각을 포함한다 (도 9 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 3C 프로테아제는, 야생형 FMDV 3C 프로테아제의 아미노산 위치들 16 및 17과 같은, 아미노산 위치들 16-25에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환들을 포함하는 피코르나바이러스 3C 프로테아제이다. 야생형 FMDV 3C 프로테아제의 위치들 16 및 17에 상응하는 부모 피코르나바이러스 3C 프로테아제에서 아미노산 위치들의 예는, 예를 들어, 야생형 리노바이러스 B (Rhinovirus B) 3C 프로테아제의 T19 및 T20 각각을 포함한다 (도 10 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩되는 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C 프로테아제의 아미노산 위치들 16, 17, 24 및 25와 같은, 아미노산 위치들 16-25에 상응하는 위치(들)에서 위치되는 하나 이상의 아미노산 치환들을 포함한다. 야생형 FMDV 3C 프로테아제의 위치들 16, 17, 24 및 25에 상응하는 부모 피코르나바이러스 3C 프로테아제에서 아미노산 위치들의 예들은, 예를 들어, 야생형 A 형 간염 바이러스(Hepatitis A virus) 3C 프로테아제의 G17, V18, G19 및 E20 각각을 포함한다 (도 11 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C 프로테아제의 아미노산 위치들 123-128과 같은, 아미노산 위치들 115-130에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환(들)을 포함한다. 야생형 FMDV 3C 프로테아제의 아미노산 위치들 123-126 및 128에 상응하는 피코르나바이러스 3C 프로테아제에서 위치들의 예들은 야생형 FMDV 3C 프로테아제와 같은 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제의 D123, V124, G125, R126 및 I128 각각을 포함한다.
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C 프로테아제의 아미노산 위치들 127 및 128과 같은, 아미노산 위치들 115-130에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환(들)을 포함한다. 야생형 FMDV 3C 프로테아제의 아미노산 위치들 124 및 127에 상응하는 피코르나바이러스 3C 프로테아제에서 위치들의 예들은 야생형 소 비염 A 바이러스 3C 프로테아제와 같은 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제의 F120 및 L123 각각을 포함한다 (도 4 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C 프로테아제의 아미노산 위치들 124 및 127과 같은, 아미노산 위치들 115-130에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환들을 포함한다. 아미노산 위치들 124 및 127에 상응하는 피코르나바이러스 3C 프로테아제에서 위치들의 예들은 야생형 소 비염 B 3C 프로테아제와 같은, 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제의 V121 및 L124 각각을 포함한다 (도 4 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C의 아미노산 위치들 125 및 127과 같은, 아미노산 위치들 115-130에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환들을 포함한다. 아미노산 위치들 125 및 127에 상응하는 피코르나바이러스 3C 프로테아제에서 위치들의 예들은 야생형 말 비염 A 3C 프로테아제과 같은, 야생형 아프토바이러스 3C 프로테아제의 A125 및 T127 각각을 포함한다(도 4 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C 프로테아제의 아미노산 위치들 117, 119, 124 및 127과 같은, 아미노산 위치들 115-130에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환들을 포함한다. 아미노산 위치들 117, 120, 124 및 127에 상응하는 피코르나바이러스 3C 프로테아제에서 아미노산 위치들의 예들은 인간 콕사키바이러스 A10과 같은 야생형 엔테로바이러스 A (Enterovirus A) 3C 프로테아제의 L102, I104, N105, M109 및 M112 각각을 포함한다 (도 5 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C의 아미노산 위치들 119, 120, 121 및 124와 같은, 아미노산 위치들 115-130에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환들을 포함한다. 아미노산 위치들 119, 120, 121 및 124에 상응하는 피코르나바이러스 3C 프로테아제에서 아미노산 위치들의 예들은 인간 엔테로바이러스 A71과 같은 야생형 엔테로바이러스 A (Enterovirus A) 3C 프로테아제의 I104, N105, T106 및 M109 각각을 포함한다 (도 6 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C 프로테아제의 아미노산 위치들 116, 118, 119, 120, 123, 126 및 128과 같은, 아미노산 위치들 115-120에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환들을 포함한다. 아미노산 위치들 116, 118, 119, 120, 123, 126 및 128에 상응하는 피코르나바이러스 3C 프로테아제에서 아미노산 위치들의 예들은 인간 콕사키바이러스 B와 같은 야생형 엔테로바이러스 B (Enterovirus B) 3C 프로테아제의 각각, L102, I104, N105, T106, F109, M112 및 I114 각각을 포함한다 (도 7 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C 프로테아제의 아미노산 위치들 117, 119, 120, 124 및 127과 같은, 아미노산 위치들 115-130에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환들을 포함한다. 아미노산 위치들 117, 119, 120, 124 및 127에 상응하는 피코르나바이러스 3C 프로테아제에서 아미노산 위치들의 예들은 인간 콕사키바이러스 A1과 같은 야생형 엔테로바이러스 C (Enterovirus C) 3C 프로테아제의 L102, V104, N105, T106, F109 및 M112 각각을 포함한다 (도 5 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C 프로테아제의 아미노산 위치들 117, 118 및 119와 같은, 아미노산 위치들 115-130에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환들을 포함한다. 아미노산 위치들 117, 118 및 119에 상응하는 피코르나바이러스 3C 프로테아제에서 아미노산 위치들의 예들은 인간 폴리오바이러스와 같은, 야생형 엔테로바이러스 C (Enterovirus C) 3C 프로테아제의 L102, I103, 및 V104 각각을 포함한다 (도 8 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C 프로테아제의 아미노산 위치들 116, 118, 122, 126 및 128과 같은, 아미노산 위치들 115-130에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환들을 포함한다. 아미노산 위치들 116, 118, 122, 126 및 128에 상응하는 피코르나바이러스 3C 프로테아제에서 위치들의 예들은 야생형 리노바이러스 A (Rhinovirus A) 3C 프로테아제의 L102, L104, Q108, T112 및 I114 각각을 포함한다 (도 9 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C 프로테아제의 아미노산 위치들 125 및 127과 같은 아미노산 위치들 115-130에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환들을 포함한다. 아미노산 위치들 128 및 130에 상응하는 피코르나바이러스 3C 프로테아제에서 아미노산 위치들의 예들은 야생형 리노바이러스 B (Rhinovirus B) 3C 프로테아제의 I112 및 E114 각각을 포함한다 (도 10 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C 프로테아제의 아미노산 위치들 115, 117, 124 및 128과 같은, 아미노산 위치들 115-130에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환들을 포함한다. 아미노산 위치들 115, 117, 124 및 128에 상응하는 피코르나바이러스 3C 프로테아제에서 아미노산 위치들의 예들은 A 형 간염 바이러스(Hepatitis A virus) 3C 프로테아제의 L119, T121, M128 및 I130 각각을 포함한다 (도 11 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C 프로테아제의 아미노산 위치들 99 및 100에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환들을 포함한다. 위치 99에 상응하는 피코르나바이러스 3C 프로테아제에서 위치들의 실시예들은 야생형 FMDV 3C 프로테아제와 같은, 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제의 I99 및 L99를 포함한다. 위치 100에 상응하는 피코르나바이러스 3C 프로테아제에서 위치들의 예들은 야생형 FMDV 3C 프로테아제와 같은, 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제의 T100을 포함한다 (도 4 및 도 12 참조).
일부 구현예들에 있어서, 본원의 상기 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 FMDV 3C 프로테아제의 아미노산 위치 100과 같은 아미노산 위치들 99 및 100에 상응하는 위치(들)에 위치되는 하나 이상의 아미노산 치환들을 포함한다. 위치 100에 상응하는 피코르나바이러스 3C 프로테아제에서 위치들의 실시예들은 야생형 소 비염 A 바이러스 3C 프로테아제와 같은 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제의 T96, 야생형 소 비염 A 바이러스 3C 프로테아제와 같은 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제의 T97, 야생형 말 비염 A 바이러스 3C 프로테아제와 같은 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제의 V100을 포함한다(도 4 및 도 12 참조).
다른 구현예들에 있어서, 야생형 FMDV 3C 프로테아제의 아미노산 위치 100에 상응하는 위치들은 예를 들어, 야생형 인간 콕사키바이러스 A10 3C 프로테아제 및 야생형 인간 엔테로바이러스 A71 3C 프로테아제와 같은, 야생형 엔테로바이러스 A 3C 프로테아제의 T87를 포함한다 (도 5, 도 6 및 도 12 참조).
다른 구현예들에 있어서, 야생형 FMDV 3C 프로테아제의 아미노산 위치 100에 상응하는 위치들은 야생형 인간 콕사키바이러스 B1 또는 B3 3C 프로테아제와 같은, 야생형 엔테로바이러스 B 3C 프로테아제의 R87을 포함한다 (도 12 참조).
다른 구현예들에 있어서, 야생형 FMDV 3C 프로테아제의 아미노산 위치 100에 상응하는 위치들은 예를 들어, 야생형 콕사키바이러스 A1 3C 프로테아제 및 야생형 인간 폴리오바이러스 3C 프로테아제와 같은, 야생형 엔테로바이러스 C 3C 프로테아제의 R87을 포함한다 (도 5, 도 8 및 도 12 참조).
다른 구현예들에 있어서, 야생형 FMDV 3C 프로테아제의 아미노산 위치 99 및 100에 상응하는 위치들은 예를 들어, 야생형 리노바이러스 A 3C 프로테아제의 I86 및 R87을 각각 포함한다 (도 9 및 도 12 참조).
다른 구현예들에 있어서, 야생형 FMDV 3C 프로테아제의 아미노산 위치 99 및 100에 상응하는 위치들은 예를 들어, 야생형 리노바이러스 B 3C 프로테아제의 I86 및 R87을 각각 포함한다 (도 10 및 도 12 참조).
다른 구현예들에 있어서, 야생형 FMDV 3C 프로테아제의 아미노산 위치 100에 상응하는 위치들은 예를 들어, 야생형 A 형 간염 바이러스 3C 프로테아제의 T100을 포함한다 (도 11 및 도 12 참조).
아미노산 치환들의 예시적 위치들
일부 측면들에 있어서, 본원은 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제와 같은, 야생형 FMDV 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 위치 I22 및 L23에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은, 예를 들어, 위치 I22 및 L23에 위치된 하나 이상의 아미노산 치환을 포함하는 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제와 같은, 변형된 FMDV 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 변형된 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제와 같은, 야생형 소 비염 A 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 위치 V22 및 C23에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은, 위치 V22 및 C23에 위치된 하나 이상의 아미노산 치환들을 포함하는 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제와 같은, 변형된 소 비염 A 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제와 같은, 야생형 소 비염 A 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 위치 Y22 및 R23에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 위치 Y22 및 R23 위치들에 위치된 하나 이상의 아미노산 치환들을 포함하는, 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제와 같은, 변형된 소 비염 B 바이러스 (Bovine rhinitis B virus) 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제와 같은, 야생형 말 비염 A 바이러스 (Equine rhinitis A virus) 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 위치 Y22 및 C23에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 위치 Y22 및 C23에 위치된 하나 이상의 아미노산 치환들을 포함하는, 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제와 같은, 변형된 말 비염 A 바이러스 (Equine rhinitis A virus) 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 엔테로바이러스 A (Enterovirus A) 3C 프로테아제와 같은, 야생형 인간 콕사키바이러스 A10 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 Q19 및 T20 위치들에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은, Q19 및 T20 위치들에 위치된 하나 이상의 아미노산 치환들을 포함하는 변형된 엔테로바이러스 A 3C 프로테아제와 같은, 변형된 인간 콕사키바이러스 A10 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은, 야생형 엔테로바이러스 A 3C 프로테아제와 같은, 야생형 인간 엔테로바이러스 A71 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 Q19 및 T20 위치들에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 Q19 및 T20 위치들에 위치된 하나 이상의 아미노산 치환들을 포함하는 변형된 엔테로바이러스 A 3C 프로테아제와 같은, 변형된 인간 엔테로바이러스 A71 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은, 야생형 엔테로바이러스 B 3C 프로테아제와 같은, 야생형 인간 콕사키바이러스 B1 및/또는 B3 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 K19 및 T20 위치들에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 K19 및 T20 위치들에 위치된 하나 이상의 아미노산 치환들을 포함하는 변형된 엔테로바이러스 B 3C 프로테아제와 같은, 변형된 인간 콕사키바이러스 B1 및/또는 B3 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 엔테로바이러스 C 3C 프로테아제와 같은, 야생형 인간 콕사키바이러스 A1 3C 프로테아제 및/또는 야생형 인간 폴리오바이러스와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 T19 및 T20 위치들에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은, T19 및 T20 위치들에 위치된 하나 이상의 아미노산 치환들을 포함하는, 변형된 엔테로바이러스 C 3C 프로테아제와 같은, 변형된 인간 콕사키바이러스 A1 3C 프로테아제 및/또는 변형된 인간 폴리오바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 리노바이러스 A 3C 프로테아제아 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 T19 및 T20 위치들에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 T19 및 T20 위치들에 위치된 상기 하나 이상의 아미노산 치환들은 포함하는 변형된 리노바이러스 A 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 리노바이러스 B 3C 프로테아제아 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 T19 및 T20 위치들에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 T19 및 T20 위치들에 위치된 상기 하나 이상의 아미노산 치환들을 포함하는 변형된 리노바이러스 B 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 A 형 간염 바이러스(Hepatitis A virus) 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 G19 및 T20 위치들에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 G19 및 G20 위치들에 위치된 상기 하나 이상의 아미노산 치환들을 포함하는 변형된 A 형 간염 바이러스(Hepatitis A virus) 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제와 같은, 야생형 FMDV 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 D123, V124, G125, R126 및 I128 위치들에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 D123, V124, G125, R126 및 I128 위치들에 위치된 상기 하나 이상의 아미노산 치환들을 포함하는 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제와 같은, 변형된 FMDV 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제와 같은, 야생형 소 비염 A 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 F120 및 L123 위치들에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면에 있어서, 본원은 위치 F120 및 L123에 위치된 하나 이상의 아미노산 치환을 포함하는 야생형 아프토바이러스 3C 프로테아제와 같은, 변형된 소 비염 A 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제와 같은, 야생형 소 비염 B 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 V121 및 L124 위치들에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 V121 및 L124 위치들에 위치된 상기 하나 이상의 아미노산 치환들을 포함하는 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제와 같은, 변형된 소 비염 B 바이러스 3C 프로테아제와 같은 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 아프토바이러스(Aphthovirus) 3C 프로테아제와 같은, 야생형 말 비염 A 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 A125 및 T127 위치들에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 A125 및 T127 위치들에 위치된 상기 하나 이상의 아미노산 치환들을 포함하는 변형된 아프토바이러스 3C 프로테아제와 같은, 변형된 말 비염 A 바이러스 3C 프로테아제와 같은 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 엔테로바이러스 A 3C 프로테아제와 같은, 야생형 인간 콕사키바이러스 A10 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 L102, I104, N105, M109 및 M112 위치들에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 L102, I104, N105, M109 및 M112 위치들에 위치된 상기 하나 이상의 아미노산 치환들을 포함하는 변형된 엔테로바이러스 A 3C 프로테아제와 같은, 변형된 인간 콕사키바이러스 A10 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 엔테로바이러스 A 3C 프로테아제와 같은, 야생형 인간 엔테로바이러스 A71 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 I104, N105, T106 및 M109 위치들에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 I104, N105, T106 및 M109 위치들에 위치된 상기 하나 이상의 아미노산 치환들을 포함하는 변형된 엔테로바이러스 A 3C 프로테아제와 같은, 변형된 인간 엔테로바이러스 A71 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 엔테로바이러스 B 3C 프로테아제와 같은, 야생형 인간 콕사키바이러스 B1 및/또는 B3 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 L102, I104, N105, T106, F109, M112 및 I114 위치들에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 L102, I104, N105, T106, F109, M112 및 I114 위치들에 위치된 상기 하나 이상의 아미노산 치환들을 포함하는 변형된 엔테로바이러스 B 3C 프로테아제와 같은, 변형된 인간 콕사키바이러스 B1 및/또는 B3 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 엔테로바이러스 C 3C 프로테아제와 같은, 야생형 인간 콕사키바이러스 A1 3C 프로테아제와 같은, 변형된 피코르나바이러스를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 L102, V104, N105, T106, F109, 및 M112 위치들에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 L102, V104, N105, T106, F109, 및 M112 위치들에 위치된 상기 하나 이상의 아미노산 치환들을 포함하는 변형된 엔테로바이러스 C 3C 프로테아제와 같은, 변형된 인간 콕사키바이러스 A1 3C 프로테아제와 같은, 변형된 피코르나바이러스에 관한 것이다.
일부 측면들에 있어서, 본원은 엔테로바이러스 C 3C 프로테아제와 같은, 야생형 인간 폴리오바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 L102, I103, 및 V104 위치들에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 L102, I103, 및 V104 위치들에 위치된 상기 하나 이상의 아미노산 치환들을 포함하는 변형된 엔테로바이러스 C 3C 프로테아제와 같은, 변형된 인간 폴리오바이러스 3C 프로테아제와 같은 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 리노바이러스 A 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 L102, L104, Q108, T112 및 I114 위치들에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 L102, L104, Q108, T112 및 I114 위치들에 위치된 상기 하나 이상의 아미노산 치환들을 포함하는 변형된 리노바이러스 A 3C 프로테아제와 같은 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 리노바이러스 B 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 I112 및 E114 위치들에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 I112 및 E114 위치들에 위치된 상기 하나 이상의 아미노산 치환들을 포함하는 변형된 리노바이러스 B 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 A 형 간염 바이러스(Hepatitis A virus) 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 L119, T121, M128 및 I130 위치들에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 L119, T121, M128 및 I130 위치들에 위치된 상기 하나 이상의 아미노산 치환들을 포함하는 변형된 A 형 간염 바이러스(Hepatitis A virus) 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 아프토바이러스 3C 프로테아제와 같은, 야생형 FMDV 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 I99, L99 및 T100 위치들에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 I99, L99 및 T100 위치들에 위치된 하나 이상의 아미노산 치환들을 포함하는 변형된 아프토바이러스 3C 프로테아제와 같은, 변형된 FMDV 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 아프토바이러스 3C 프로테아제와 같은, 야생형 소 비염 A 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 T96 위치에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 T96 위치에 위치된 하나 이상의 아미노산 치환들을 포함하는 변형된 아프토바이러스 3C 프로테아제와 같은, 변형된 소 비염 A 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 아프토바이러스 3C 프로테아제와 같은, 야생형 소 비염 B 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 T97 위치에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 T97 위치에 위치된 하나 이상의 아미노산 치환들을 포함하는 변형된 아프토바이러스 3C 프로테아제와 같은, 변형된 소 비염 B 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 아프토바이러스 3C 프로테아제와 같은, 야생형 말 비염 A 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 V100 위치에 위치된 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 V100 위치에 위치된 하나 이상의 아미노산 치환들을 포함하는 변형된 아프토바이러스 3C 프로테아제와 같은, 변형된 말 비염 A 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 엔테로바이러스 A 3C 프로테아제와 같은, 야생형 인간 콕사키바이러스 A10 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 T87 위치에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 T87 위치에서 하나 이상의 아미노산 치환들을 포함하는 변형된 엔테로바이러스 A 3C 프로테아제와 같은, 변형된 인간 콕사키바이러스 A10 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제와 같은 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 엔테로바이러스 A 3C 프로테아제와 같은, 야생형 인간 엔테로바이러스 A71 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 T87 위치에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 T87 위치들에서 하나 이상의 아미노산 치환들을 포함하는 변형된 엔테로바이러스 A 3C 프로테아제와 같은, 변형된 인간 엔테로바이러스 A71 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제와 같은 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 엔테로바이러스 B 3C 프로테아제와 같은, 야생형 인간 콕사키바이러스 B1 및/또는 B3 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 R87 위치에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 R87 위치에서 하나 이상의 아미노산 치환들을 포함하는 변형된 엔테로바이러스 B 3C 프로테아제와 같은, 변형된 인간 콕사키바이러스 B1 및/또는 B3 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제와 같은 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 엔테로바이러스 C 3C 프로테아제와 같은, 야생형 인간 콕사키바이러스 A1 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 R87 위치에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 R87 위치에서 하나 이상의 아미노산 치환들을 포함하는 변형된 엔테로바이러스 C 3C 프로테아제와 같은, 변형된 인간 콕사키바이러스 A1 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제와 같은 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 야생형 엔테로바이러스 C 3C 프로테아제와 같은, 야생형 인간 폴리오바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 R87 위치에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 R87 위치에서 하나 이상의 아미노산 치환들을 포함하는 변형된 엔테로바이러스 C 3C 프로테아제와 같은, 변형된 인간 폴리오바이러스(Human poliovirus) 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 리노바이러스 A 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 I86 및 R87 및 I114 위치들에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 I86 및 R87 및 I114 위치들에서 하나 이상의 아미노산 치환들을 포함하는 변형된 리노바이러스 A 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제와 같은 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 리노바이러스 B 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 I86 및 R87 위치들에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 I86 및 R87 위치들에서 하나 이상의 아미노산 치환들을 포함하는 변형된 리노바이러스 B 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제와 같은 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
일부 측면들에 있어서, 본원은 A 형 간염 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 관한 것이고, 여기서 상기 변형된 피코르나바이러스 3C 프로테아제는 T100 위치에서 하나 이상의 아미노산 치환들을 포함한다. 다른 측면들에 있어서, 본원은 T100 위치에서 하나 이상의 아미노산 치환들을 포함하는 변형된 A 형 간염 바이러스 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제에 관한 것이다.
아미노산 치환들
본원에 기술된 상기 아미노산 치환을 수득하는 돌연변이들은 본원에 기술된 상기 야생형 피코르나바이러스 3C 프로테아제와 같은 부모 피코르나바이러스 3C 프로테아제 또는 본원에 기술된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드에 본 기술분야에 공지된 임의의 방법론을 사용하여 도입될 수 있다. 돌연변이들은 무작위로, 예를 들어, 2가 금속 이온 보조인자(cofactor)로서 망간의 존재하에 PCR 반응을 수행함으로써 도입될 수 있다. 다른 일부 구현예들에 있어서, 올리고뉴클레오타이드-지시 돌연변이는 본원의 상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 폴리뉴클레오타이드를 생성하는데 사용될 수 있고, 이것은 코딩 DNA 분자를 따라 임의의 결정된 부위에서 염기쌍 변화의 가능한 모든 부류를 허용한다. 일반적으로, 상기 기술은, 상기 목적 부모 피코르나바이러스 3C 프로테아제를 코딩하는 단일 가닥 뉴클레오타이드 서열에 상보적(하나 이상의 미스매치를 제외)인 올리고뉴클레오타이드를 어닐링(annealing)하는 것을 포함한다. 그 후, 상기 미스매치된 올리고뉴클레오타이드는 DNA 폴리머라아제에 의해 연장되어, 한 가닥의 서열로 요구되는 아미노산 치환을 포함하는 이중-가닥 DNA 분자를 생성한다. 그 후, 상기 이중-가닥 폴리뉴클레오타이드는 적절한 발현 벡터에 삽입될 수 있고, 이에 따라 돌연변이체 또는 변형된 폴리펩타이드를 생산할 수 있다. 상기 전술한 올리고뉴클레오타이드-지시 돌연변이 또는 부위-지시 돌연변이유발은 선택적으로, PCR을 통해 수행될 수 있다.
상기 아미노산 치환은 아르기닌, 알라닌, 아스파라긴, 아르파라긴 산, 시스테인, 글루타민, 글루타민 산, 글리신, 히스티딘, 이소류신, 류신, 라이신, 메티오닌, 페닐알라닌, 프롤린, 세린, 트레오닌, 트립토판, 티로신, 발린, 또는 비천연 아미노산을 포함할 수 있다. 예를 들어, 야생형 리노바이러스 B 3C 프로테아제로부터 3C 프로테아제 내에 R87은 알라닌, 아스파라긴, 아르파라긴 산, 시스테인, 글루타민, 글루타민 산, 글리신, 히스티딘, 이소류신, 류신, 라이신, 메티오닌, 페닐알라닌, 프롤린, 세린, 트레오닌, 트립토판, 티로신, 발린, 또는 비천연 아미노산으로 대체될 수 있다.
일부 구현예들에 있어서, 하나 이상의 아미노산 치환들은 하나 이상의 비-보존적 치환을 포함한다. 비-보존적 아미노산 치환은 상이한 화학적 성질 (예를 들어, 극성, 수소 결합 가능성, 산성, 염기성, 형태, 소수성, 방향족 특성 등)을 갖는 다른 아미노산으로 아미노산을 대체하는 것을 의미한다. 예들은 Ala, Val, Leu, Ile, Pro, Phe, Trp 또는 Met와 같은 소수성, 비극성 아미노산을, Ser, Thr, Cys, Tyr, Asn 또는 Gln과 같은 친수성, 극성 아미노산, 또는 Asp 또는 Glu와 같은 산성의 음전하를 띈 아미노산, Lys, Arg 또는 His와 같은 염기성의 양전하를 띈 아미노산으로 대체하는 것을 포함한다. 비-보존적 치환의 다른 예들은 하기 표 1의 하기 그룹들 사이에서 아미노산을 치환하는 것을 포함한다.
[표 1] 아미노산 사이드 체인 특성
Figure pct00001
일부 구현예들에 있어서, 하나 이상의 아미노산 치환들은 프롤린을 포함한다. 본 기술분야에 공지된 것과 같이, 프롤린은 전형적으로 알파 나선 및 베타 시트를 포함하는 2차 구조 요소의 구조 파괴자(disrupter)로서 작용한다.
일부 구현예들에 있어서, 본원에 기술된 상기 단리된 폴리뉴클레오타이드에 의해 코딩되는 상기 변형된 피코르나바이러스 3C 프로테아제 및 본원의 변형된 피코르나바이러스 3C 프로테아제는 1-19 아미노산 치환들과 같은 1-27 아미노산 치환, 예를 들어, 1-4 또는 1-3 아미노산 치환들과 같은, 1 또는 2 아미노산 치환들과 같은 1-10, 1-8, 1-6, 1-5 아미노산 치환들을 포함한다.
일부 구현예들에 있어서, 하나 이상의 아미노산 치환(들)은 본원의 변형된 피코르나바이러스 3C 프로테아제의 상기 단백질 형태 및/또는 기능적 특성을 변경시키지 않는다. 그러나, 보다 전형적으로, 상기 하나 이상의 아미노산 치환(들)은 본원의 변형된 3C 프로테아제의 상기 형태 및/또는 기능적 특성을 변경시킨다. 전형적으로, 본원의 3C 프로테아제의 상기 형태 및 하나 이상의 기능적 특성은 대조군 3C 프로테아제의 것과 비교하여 변형된다.
일부 구현예들에 있어서, 본원에 기술된 상기 하나 이상의 아미노산 치환(들)은 알파 나선, 베타 시트, 베타 가닥 또는 루프와 같은, 2차 구조를 변경시킨다. 2차 구조의 변경은 SPIDER2, Heffernan 등, "Highly Accurate Sequence-based Prediction of Half-Sphere Exposures of Amino Acid Residues in Proteins", Bioinformatics 2016, 32: 843- 849 및 PSIPRED Jones DT. "Protein secondary structure prediction based on position-specific scoring matrices", J. Mol. Biol., 9, 292: 195-202]을 포함하여 잘 알려진 단백질 2차 구조 예측 프로그램을 이용하여 결정될 수 있고, 그 전문은 본원에서 참조로서 포함된다.
일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제는, 번역된 아미노산 서열에서 하나 이상의 아미노산 치환들, 구체적으로 A1-B1 β 시트, A2-B2 β 시트 및/또는 F1 β 가닥 및 A2 β 가닥 사이의 루프, 또는 A1, A2, B1 및/또는 B2 β 가닥 중 하나 이상을 표적으로 하는 하나 이상의 점 돌연변이를 초래할 피코르나바이러스 3C 프로테아제를 코딩하는 뉴클레오타이드 서열에 하나 이상의 점 돌연변이를 포함한다.
일부 구현예들에 있어서, 상기 A1-B1 시트들을 변경, 예를 들어, 파괴하는 상기 하나 이상의 아미노산 치환들은 야생형 FMDV 3C 프로테아제의 하나 이상의 아미노산 위치들 16-25에 상응하는 위치(들)에 위치된다.
일부 구현예들에 있어서, 상기 A1-B1 시트들을 변경, 예를 들어, 파괴하는 상기 하나 이상의 아미노산 치환들은 상기 A1 β 시트를 변경, 예를 들어, 파괴시킨다. 전형적으로, 상기 A1 β 시트를 변경, 예를 들어, 파괴하는 상기 하나 이상의 아미노산 치환들은 야생형 FMDV 3C 프로테아제의 하나 이상의 아미노산 위치들 20-23에 상응하는 위치(들)에 위치된다.
일부 구현예들에 있어서, 상기 A1-B1 시트들을 변경, 예를 들어, 파괴하는 상기 하나 이상의 아미노산 치환들은 상기 A1 β 가닥과 B1 β 가닥 사이의 루프를 변경, 예를 들어, 파괴시킨다. 전형적으로, 상기 A1 β 가닥과 B1 β 가닥 사이의 상기 루프를 변경, 예를 들어, 파괴하는 상기 하나 이상의 아미노산 치환들은 야생형 FMDV 3C 프로테아제의 하나 이상의 아미노산 위치들 24 및 25에 상응하는 위치(들)에 위치된다.
일부 구현예들에 있어서, 상기 F1 β 가닥과 상기 A2 β 가닥 사이의 상기 루프를 변경, 예를 들어, 파괴하는 하나 이상의 아미노산 치환들은 야생형 FMDV 3C 프로테아제의 하나 이상의 아미노산 위치들 99 및 100에 상응하는 위치(들)에 위치된다.
일부 구현예들에 있어서, 상기 A2-B2 β 시트를 변경, 예를 들어, 파괴하는 하나 이상의 아미노산 치환들은 야생형 FMDV 3C 프로테아제의 하나 이상의 아미노산 위치들 115-130에 상응하는 위치(들)에 위치된다.
일부 구현예들에 있어서, 하나 이상의 아미노산 치환들은 상기 A2-B2 β 시트의 상기 B2 β 가닥을 변경, 예를 들어, 파괴한다. 일부 구현예들에 있어서, 상기 B2 β 가닥은 FMDV 3C 프로테아제와 같은 피코르나바이러스 3C 프로테아제의 잔기 123 내지 128을 포함한다. 일부 구현예들에 있어서, 상기 A2-B2 β 시트의 상기 B2 β 가닥을 변경, 예를 들어, 파괴하는 하나 이상의 아미노산 치환들은 야생형 FMDV 3C 프로테아제의 하나 이상의 잔기 123 내지 128에 상응하는 위치(들)에 위치된다.
일부 구현예들에 있어서, 상기 하나 이상의 아미노산 치환들은 상기 B2' β 가닥을 분열시킨다. 상기 B2' β 가닥은 소 비염 A 바이러스, 소 비염 B 바이러스, 말 비염 A 바이러스 및 FMDV를 포함하는 상기 아프토바이러스 속 내에 종들에서 잔기 130 내지 133에 상응한다. 리노바이러스 A, 리노바이러스 B, 및 A 형 간염 바이러스를 포함하는 종들과 같은 일부 구현예들에 있어서, 상기 B2' β 가닥은 존재하지 않는다. 예를 들어, 상기 A2-B2 β 시트의 상기 B2' β 가닥은 잔기 103 내지 116 (리노바이러스 A) 및 잔기 100 내지 114 (리노바이러스 B)에 상응하고, FMDV 에서 잔기 117 내지 130에 상동성이다. A 형 간염 바이러스(Hepatitis A virus)로부터 3C 프로테아제와 같은, 일부 구현예들에 있어서, 상기 A2-B2 β 시트의 상기 B2 β 가닥은 잔기 121 내지 132에 상응하고, FMDV에서 잔기 120 내지 132에 상동성이다. 따라서, 일부 구현예들에 있어서, 상기 A2-B2 β 시트를 변경, 예를 들어, 파괴하는 하나 이상의 치환들은 야생형 FMDV 3C 프로테아제의 아미노산 위치들 117 내지 130에 상응하는 위치(들)에 위치된다. 다른 구현예들에 있어서, 상기 A2-B2 β 시트를 변경 예를 들어, 파괴하는 하나 이상의 치환들은 아미노산 위치들 120 내지 130과 같은 야생형 FMDV 3C 프로테아제의 하나 이상의 위치들 120 내지 132에 상응하는 위치(들)에 위치된다.
일부 구현예들에 있어서, A1-B1 시트와 같은, A2-B2 β 시트 및/또는 F1 β 가닥과 A2 β 가닥 사이의 루프와 같은 2차 구조의 변경, 예를 들어 파괴하는 대조군 3C 프로테아제의 것과 비교하여, 기능적 특성의 변화와 연관된다. 예를 들어, 일부 구현예들에 있어서, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는, 대조군 3C 프로테아제의 것과 비교하여, 숙주 세포에서 발현될 때, 감소된 독성 및/또는 P1 전구체 폴리펩타이드와 같은 폴리펩타이드의 형질전환 유전자 발현의 향상을 가능하게 한다. 일부 구현예들에 있어서, 본원의 상기 변형된 피코르나바이러스 3C 프로테아제의 상기 단백질 분해 가공 능력은 대조군 3C 프로테아제의 것과 비교하여 감소된다.
피코르나바이러스 단백질 분해 가공 동안, 피코르나바이러스 RNA 게놈의 단일 개방 판독 프레임으로부터 번역될 수 있는, 단일 폴리펩타이드 전구체는 야생형 피코르나바이러스 3C 프로테아제를 포함하는 바이러스 코딩된 프로테아제에 의해 기능적 단백질로 절단된다. 전형적으로, 절단은 도 2에 나타낸 것과 같이 상이한 단계들에서 일어나고, 구조 단백질 VP1, VP2, VP3 및 VP4 뿐만 아니라 비-구조 단백질 L, 2A, 2B, 2C, 3A, 3B1, 3B2, 3B3, 3C 및 3D를 형성하기 위해 추가 절단되는 P1, P2 및 P3을 포함하는 다중 중간체 폴리펩타이드 전구체들을 형성한다.
P1은 전형적으로 야생형 피코르나바이러스 3C 프로테아제에 의한 성숙 바이러스의 조립 동안 절단되어 VP0 VP1 및 VP3을 생성하고, 자기 조립되어 대략 구형의 단백질 쉘 (캡시드)을 형성한다. 전형적으로, VP2 및 VP4로의 VP0의 자기 촉매성 절단은 성숙한 바이러스를 생성하기 위한 상기 바이러스 게놈의 캡슐화 동안 발생한다. 상기 캡시드는, 일반적으로 바이러스가 세포 외부에 있는 동안 상기 게놈을 보호하는 역할을 하고, 또한 특정 세포-표면 수용체 (예: 특정 인테그린)와의 상호 작용을 통해 그것이 세포에 결합하고 그 후 세포로 진입하는 것을 허용한다. 상기 바이러스 캡시드는 VP1, VP2, VP3 및 VP4 각각의 수많은 복제, 예를 들어 60 개의 복제로 구성될 수 있다.
본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제 및/또는 본원의 변형된 피코르나바이러스 3C 프로테아제의 단백질 분해 활성은 대조군 3C 프로테아제의 것보다 더 크거나 더 작을 수 있다. 일부 구현예들에 있어서, 본원의 변형된 피코르나바이러스 3C 프로테아제의 상기 단백질 분해 활성은 대조군 피코르나바이러스 3C 프로테아제의 상기 단백질 분해 활성의 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 110%, 120%, 130%, 140%, 150% 또는 200%를 나타낸다. 상기 활성은 VP0, VP1 및 VP3 바이러스 단백질, 또는 일부 구현예들에 있어서, VP1, VP2, VP3 및 VP4를 생산하는 것과 같은, P1의 하나 이상의 절단을 수행하기 위한 상기 변형된 피코르나바이러스 3C 프로테아제의 상대적인 능력에 기초하여 결정될 수 있다, 일 비제한적 예에 있어서, 상기 변형된 피코르나바이러스 3C 프포테아제는, 가장 가까운 천연 대립 유전자와 같은, 대조군 피코르나바이러스 3C 프로테아제의 것과 비교하여, P1을 절단하는 능력이 적어도 90%를 나타낸다.
추가 구현예들에 있어서, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제 또는 본원의 변형된 피코르나바이러스 3C 프로테아제는 대조군 피코르나바이러스 3C 프로테아제와 비교하여, 숙주 세포에서 발현되는 피코르나바이러스 P1 폴리펩타이드 전구체의 총량의 적어도 90%, 적어도 95%와 같이, 또는 적어도 99%와 같이, 또는 적어도 99.9%와 같이, 예를 들어, 99.9% 내지 100%와 같은 가공 능력을 가진다.
통상적이고 유리하게, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제 및/또는 본원에 기술된 상기 변형된 피코르나바이러스 3C 프로테아제는 피코르나바이러스 P1 폴리펩타이드 전구체를 전체 가공하여, 개별적인 캡시드 단백질 VP1, VP2, VP3 및 VP4 또는 VP0, VP1 및 VP3로 절단하는 능력을 유지하고, 이어서 숙주 세포에서 상기 절단된 바이러스 캡시드 단백질을 빈 피코르나바이러스 캡시드로의 조립을 허용한다.
일부 구현예들에 있어서, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제 및/또는 본원에 기술된 상기 변형된 피코르나바이러스 3C 프로테아제는 P1, P2 및 P3를 포함하는 단일 피코르나바이러스 폴리프로테인 번역 생성물의 총량의 적어도 90%를 가공하는 능력을 갖는다. 일부 구현예들에 있어서, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제 및/또는 본원에 기술된 상기 변형된 피코르나바이러스 3C 프로테아제는 P2 (또는 2ABC), P3 (또는 3ABCD), P1 (또는 1ABCD), 1ABC, 2BC, 3AB, 및 3CD와 같은 피코르나바이러스 폴리펩타이드 전구체의 총량의 적어도 90%를 가공하기 위한 능력을 가진다 (도 1 참조). 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제 및/또는 본원에 기술된 상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 피코르나바이러스 단백질에서의 본래 부위 및/또는 피코르나바이러스 3C 절단 부위를 포함하기 위해 조작된 단백질과 같은, 비-피코르나바이러스 단백질들에서 동일하거나 또는 유사한 3C 절단 부위를 절단하거나 가공하기 위해 사용될 수 있다. 일부 구현예에 있어서, 상기 피코르나바이러스 폴리펩타이드 전구체들은 피코르나바이러스 P1 폴리펩타이드 전구체를 포함하지 않는다.
일부 구현예들에 있어서, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제 및/또는 본원에 기술된 상기 변형된 피코르나바이러스 3C 프로테아제는 대조군 3C 프로테아제와 비교하여, 숙주 세포에서 상기 피코르나바이러스 3C 프로테아제의 독성을 감소하거나 제거한다.
예를 들어, 숙주 세포의 성장률 또는 형질전환된 숙주 세포에서 코딩되고 발현된 적어도 하나의 단백질의 수율은 대조군 피코르나바이러스 3C 프로테아제를 발현하는 숙주 세포의 성장률 또는 적어도 하나의 단백질 수율과 비교할 때, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩되는 상기 변형된 피코르나바이러스 3C 프로테아제가 발현되고 및/또는 본원에 기술된 상기 변형된 피코르나바이러스 3C 프로테아제가 숙주 세포에서 적어도 1, 2, 3, 4, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, 200, 300% 또는 그 이상 증가될 것이다.
일부 구현예들에 있어서, 피코르나바이러스 3C 프로테아제의 변형은 본원의 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 돌연변이 뉴클레오타이드 서열 및 피코르나바이러스 P1 폴리펩타이드 전구체를 코딩하는 뉴클레오타이드 서열을 적어도 포함하는 형질전환 유전자 발현 카세트 및/또는 재조합 발현 벡터의 발현 산물(output)을 증가시킬 수 있다, 일부 비제한적인 구현예들에 있어서, 상기 형질전환 유전자 발현 산물은, 대조군 피코르나바이러스 3C 프로테아제를 코딩하는 뉴클레오타이드 서열 및 피코르나바이러스 P1 폴리펩타이드 전구체를 코딩하는 뉴클레오타이드 서열을 포함하는 형질전환 유전자 발현 카세트 및/또는 재조합 발현 카세트의 것과 비교하여, 20 배 이하, 2-20 배와 같은, 5-15 배와 같은, 10-15 배와 같은 양 만큼 증가한다. 숙주 세포가 이러한 형질전환 유전자 발현 카세트 및/또는 재조합 발현 벡터로 형질주입될 때, 형질전환 유전자 발현 생산량의 증가는 숙주 세포에서의 바이러스-유사 입자 (VLPs)의 생산 증가로 이어진다.
일부 구현예들에 있어서, 상기 형질전환 유전자 발현 생산량(output)은, 상기 형질전환 유전자 발현 카세트에, 가우시아(Gaussia) 루시페라아제 유전자 (GLuc) 또는 비제한적으로 SGLuc를 포함하는 그의 변이체와 같은, 발광 리포터 유전자를 융합한 후 조도계 (luminometer)에서 0.5 초의 집적 시간을 이용하여 상대적인 발광 단위 (RLU)의 양을 측정함으로써 평가될 수 있다. 일부 비제한적인 구현예들에 있어서, 형질전환 유전자 발현 카세트를 포함하는 재조합 발현 벡터, 또는 적어도 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 돌연변이체 뉴클레오타이드 서열 및 피코르나바이러스 P1 폴리펩타이드 전구체를 코딩하는 뉴클레오타이드를 포함하는 재조합 발현 벡터는 숙주 세포에서 109-1010 RLU/0.5s, 전형적으로 2x109 내지 8x1010 RLU/0.5s, 보다 전형적으로 4x109 내지 3x1010 RLU/0.5s의 형질전환 유전자 발현 생산량을 갖는다.
다른 일부 구현예들에 있어서, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제를 발현하는 숙주 세포의 생존 가능한 능력 또는 계대(passage) 능력 (세대 대 세대에 걸쳐 형질전환 유전자를 안정하게 유지 및 발현하는 숙주 세포의 능력) 은 대조군 피코르나바이러스 3C 프로테아제를 발현하는 다른 상동성 숙주 세포와 비교하여 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, 200% 또는 그 이상 증가할 수 있다.
본원의 단리된 폴리뉴클레오타이드에 의해 코딩되는 상기 변형된 피코르나바이러스 3C 프로테아제는, 하나 이상의 숙주 단백질 표적 부위에서 하나 이상의 숙주 세포 단백질 또는 형질전환 유전자에 의해 코딩된 것과 같은 다른 공동-발현된 단백질에 대하여 대조군 피코르나바이러스 3C의 단백질 분해 활성의 100, 90, 80, 70, 60, 50, 40, 30, 20 또는 10% 미만을 나타내며, 피코르나바이러스 P1 전구체 단백질을 배제한 피코르나바이러스 P1 전구체 단백질 또는 피코르나바이러스 폴리펩타이드 전구체와 같은, 하나 이상의 피코르나바이러스 폴리펩타이드 전구체를 가공하는 현저한 능력을 유지한다.
일부 구현예들에 있어서, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는, 숙주 세포 단백질에 대한 단백질 분해 활성이 100, 90, 80, 70, 60, 50, 40,30, 20 또는 10% 미만, 또는 10% 이하와 같은 것을 나타내며, 상기 숙주 단백질은, 진핵생물 번역 개시 인자 4A1 (eIF4A1), 히스톤 H3, 핵 전사 인자 카파 B 필수 조절자 (NEMO), 유사 분열에서 68 kDa의 Src-연관 기질 (SAM68), 진핵생물 세포 번역 개시 인자 4G (eIF4G)를 비제한적으로 포함한다. 일부 구현예들에 있어서, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제의 존재는, 대조군 피코르나바이러스 3C 프로테아제를 발현하는 다른 상동성 숙주 세포와 비교하여, 숙주 세포에서 골지(Golgi) 단편화를 덜 나타내고 및 미세소관 시스템 집적의 손실을 감소시킨다.
일부 구현예들에 있어서, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제는, 다른 상동성의 변형되지 않은 피코르나바이러스 3C 프로테아제, 즉 대조군 피코르나바이러스 3C 프로테아제와 비교하여, eIF4A1 진핵 세포 개시 인자에 대한 단백질 분해 활성이 감소될 것이다. 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제는, 대조군 피코르나바이러스 3C 프로테아제의 것과 비교하여, eIF4A1의 진핵 개시 인자 0.001, 0.01, 0.1, 1, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90 또는 95% 만을 분해시킬 것이다. 일부 구현예들에 있어서, 이러한 감소된 활성은 숙주 세포에서 발현된 eIF4A1의 총량을 기준으로 10% 이하, 예를 들어, 8% 이하, 예를 들어, 5% 이하, 예를 들어, 1% 이하, 및 0.001% 내지 1.0%이다.
본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제는 대조군 피코르나바이러스 3C 프로테아제의 것과 비교하여, 히스톤 H3, 핵 전사 인자 카파 B 필수 조절자 (NEMO), 유사 분열에서 68 kDa의 Src-연관 기질 (SAM68) 및/또는 진핵생물 번역 개시 인자 4G (eIF4G)의 적어도 하나에 대한 활성 손실을 나타낼 것이다. 본원의 상기 변형된 3C 프로테아제는, 다른 상동성의 변형되지 않은 피코르나바이러스 3C 프로테아제, 즉, 대조군 피코르나바이러스 3C 프로테아제의 것과 비교하여, 히스톤 H3, 핵 전사 인자 카파 B 필수 조절자 (NEMO), 유사 분열에서 68 kDa의 Src-연관 기질 (SAM68) 및/또는 진핵생물 번역 개시 인자 4G (eIF4G)의 적어도 하나의 0.001, 0.01, 0.1, 1, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90 또는 95%만을 분해시킬 것이다. 추가 구현예들에 있어서, 숙주 세포의 골지(Golgi) 단편화 및 미세소관 시스템 집적의 손실을 유도하는 변형된 피코르나바이러스 3C 프로테아제의 능력은, 대조군 피코르나바이러스 3C 프로테아제의 것과 비교하여, 본원의 하나 이상의 돌연변이들에 의해 감소되거나 제거될 수 있다.
예시적 피코르나바이러스 변형된 3C 프로테아제
일부 구현예들에 있어서, 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제, 예를 들어, 변형된 FMDV 3C 프로테아제와 같은, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제는, 서열 번호 2 (A Turkey 2006), 서열 번호 4 (A24 Cruzeiro iso71), 서열 번호 6 (Asia Lebanon 89, 혈청형 Asia 1), 및 서열 번호 8 (Asia 1 Shamir), 서열 번호 10 (C3 Indaial), 서열 번호 12 (O1 PanAsia), 서열 번호 14 [O1 Manisa 단리물(isolate) 87 균주, 혈청형 O], 서열 번호 16 (SAT1-20 iso11), 서열 번호 18 (SAT2 이집트 2010), 및 서열 번호 20 (SAT3 ZIM/6/91) 중에서 선택되는 야생형 FMDV 3C 프로테아제와 적어도 90%, 적어도 95%, 적어도 99% 상동성이다.
일부 구현예들에 있어서, 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제, 예를 들어, 변형된 소 비염(Bovine rhinitis) 3C 프로테아제와 같은, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는, 서열 번호 422 [소 비염 A (Bovine rhinitis A) Sd-1] 및 서열 번호 434 [소 비염 B (Bovine rhinitis B)] 중에서 선택된 야생형 소 비염(Bovine rhinitis) 3C 프로테아제와 적어도 90%, 적어도 95%, 적어도 99% 상동성이다.
일부 구현예들에 있어서, 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제, 예를 들어, 변형된 말 비염 A (Equine rhinitis A) 3C 프로테아제와 같은, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는, 서열 번호 444의 야생형 말 비염 A (Equine rhinitis A) 3C 프로테아제와 적어도 90%, 적어도 95%, 적어도 99% 상동성이다.
일부 구현예들에 있어서, 엔테로바이러스 A 3C 프로테아제와 같은 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는, 서열 번호 478의 상기 야생형 인간 콕사키바이러스 A10 3C 프로테아제 및 서열 번호 526의 상기 야생형 인간 엔테로바이러스 A71 3C 프로테아제 중에서 선택되는 야생형 엔테로바이러스 A 3C 프로테아제와 적어도 90%, 적어도 95%, 적어도 99% 상동성이다.
일부 구현예들에 있어서, 엔테로바이러스 B 3C 프로테아제와 같은, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는, 서열 번호 494의 상기 야생형 인간 콕사키바이러스 B1 3C 프로테아제 및 서열 번호 510의 상기 인간 콕사키바이러스 B3 (인간 콕사키바이러스 B3 마코시) 중에서 선택되는 야생형 엔테로바이러스 B 3C 프로테아제와 적어도 90%, 적어도 95%, 적어도 99% 상동성이다.
일부 구현예들에 있어서, 엔테로바이러스 C 3C 프로테아제와 같은, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는, 서열 번호 462의 상기 야생형 인간 콕사키바이러스 A1 3C 프로테아제(인간 콕사키바이러스 A1 Tompkins), 및 서열 번호 552 (인간 폴리오바이러스 2 균주 R93152), 서열 번호 565 (인간 폴리오바이러스 3 단리물 CHN5275/JX/CHN/2001) 및 서열 번호 580 (인간 폴리오바이러스 Mahoney) 중에서 선택되는 야생형 인간 폴리오바이러스 3C 프로테아제 중에서 선택되는 야생형 엔테로바이러스 C 3C 프로테아제와 적어도 90%, 적어도 95%, 적어도 99% 상동성이다.
일부 구현예들에 있어서, 변형된 리노바이러스 A 3C 프로테아제와 같은, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제는, 서열 번호 604 (인간 리노바이러스 A20, 균주 VR1130)의 야생형 인간 리노바이러스, 서열 번호 618 (인간 리노바이러스 A89, 균주 VR1199)의 야생형 인간 리노바이러스 및 서열 번호 620 (인간 리노바이러스 A2)의 야생형 인간 리노바이러스 A 3C 중에서 선택되는 야생형 리노바이러스 A 3C 프로테아제와 적어도 90%, 적어도 95%, 적어도 99% 상동성이다.
일부 구현예들에 있어서, 변형된 리노바이러스 B 3C 프로테아제와 같은, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제는, 서열 번호 594 (인간 리노바이러스 B14)의 야생형 리노바이러스 B 3C 프로테아제와 적어도 90%, 적어도 95%, 적어도 99% 상동성이다.
일부 구현예들에 있어서, 변형된 헤파토바이러스(Hepatovirus) 3C 프로테아제와 같은, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제는, 서열 번호 538 (A 형 인간 간염 바이러스)의 야생형 헤파토바이러스(Hepatovirus)와 적어도 90%, 적어도 95%, 적어도 99% 상동성이다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제는, 서열 번호 22 (A Turkey 2006, D123P), 서열 번호 24 (A24 Cruzeiro iso71, D123P), 서열 번호 26 (Asia Lebanon 89, 혈청형 Asia 1, D123P), 서열 번호 28 (Asia1 Shamir, D123P), 서열 번호 30 (C3 Indaial, D123P), 서열 번호 32 (O1 PanAsia, D123P), 서열 번호 34 (O1 Manisa 단리물 87 균주, 혈청형 O, D123P), 서열 번호 36 (SAT1-20 iso11, D123P), 서열 번호 38 (SAT2 Egypt 2010, D123P) 및 서열 번호 40 (SAT3 ZIM/6/91, D123P) 중에서 선택되는 변형된 FMDV 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 본원의 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩되는 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 42 (A Turkey 2006, G125P), 서열 번호 44 (A24 Cruzeiro iso71, G125P) 서열 번호 46 (Asia Lebanon 89, 혈청형 Asia1, G125P), 서열 번호 48 (Asia 1 Shamir, G125P), 서열 번호 50 (C3 Indaial, G125P), 서열 번호 52 (O1 PanAsia, G125P), 서열 번호 54 (O1 Manisa 단리물 87 균주, 혈청형 O, G125P), 서열 번호 56 (SAT1-20 iso11, G125P), 서열 번호 58 (SAT2 Egypt 2010, G125P) 및 서열 번호 60 (SAT3 ZIM/6/91, G125P) 중에서 선택되는 변형된 FMDV 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 62 (A Turkey 2006, I128P), 서열 번호 64 (A24 Cruzeiro iso71, I128P), 서열 번호 66 (Asia Lebanon 89, 혈청형 Asia 1, G125P), 서열 번호 68 (Asia1 Shamir, I128P), 서열 번호 70 (C3 Indaial, I128P) 서열 번호 72 (O1 PanAsia, G125P), 서열 번호 74 (O1 Manisa 단리물 87 균주, 혈청형 O, I128P), 서열 번호 76 (SAT1-20 iso11, I128P), 서열 번호 78 (SAT2 Egypt 2010, I128P) 및 서열 번호 80 (SAT3 ZIM/6/91, I128P) 중에서 선택되는 변형된 FMDV 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 82 (A Turkey 2006, I22P), 서열 번호 84 (A24 Cruzeiro iso71, I22P), 서열 번호 86 (Asia Lebanon 89, 혈청형 Asia 1, I22P), 서열 번호 88 (Asia 1 Shamir, I22P), 서열 번호 90 (C3 Indaial, I22P), 서열 번호 92 (O1 PanAsia, I22P), 서열 번호 94 (O1 Manisa 단리물 87 균주, 혈청형 O, I22P), 서열 번호 96 (SAT1-20 iso11, I22P), 서열 번호 98 (SAT2 Egypt 2010, I22P), 및 서열 번호 100 (SAT3 ZIM/6/91, I22P) 중에서 선택되는 변형된 FMDV 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 102 (A Turkey 2006, I99P), 서열 번호 104 (A24 Cruzeiro iso71, I99P), 서열 번호 106 (Asia Lebanon 89, 혈청형 Asia 1, I99P), 서열 번호 108 (Asia1 Shamir, I99P), 서열 번호 110 (C3 Indaial, I99P), 서열 번호 112 (O1 PanAsia, I99P), 서열 번호 114 (O1 Manisa 단리물 87 균주, 혈청형 O, I99P), 서열 번호 116 (SAT2 Egypt 2010, I99P), 서열 번호 178 (SAT1-20 iso11, I99P) 및 서열 번호 180 (SAT3 ZIM/6/91, I99P) 중에서 선택되는 변형된 FMDV 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 138 (A Turkey 2006, L23P), 서열 번호 140 (A24 Cruzeiro iso71, L23P), 서열 번호 142 (Asia Lebanon 89, 혈청형 Asia 1, L23P), 서열 번호 144 (Asia1 Shamir, L23P), 서열 번호 146 (C3 Indaial, L23P), 서열 번호 148 (O1 PanAsia, L23P), 서열 번호 150 (O1 Manisa 단리물 87 균주, 혈청형 O, L23P), 서열 번호 152 (SAT1-20 iso11, L23P), 서열 번호 154 (SAT2 Egypt 2010, L23P) 및 서열 번호 156 (SAT3 ZIM/6/91, L23P) 중에서 선택되는 변형된 FMDV 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 182 (A Turkey 2006, R126P), 서열 번호 184 (A24 Cruzeiro iso71, R126P), 서열 번호 186 (Asia Lebanon 89, 혈청형 Asia 1, R126P), 서열 번호 188 (Asia1 Shamir, R126P), 서열 번호 190 (C3 Indaial, R126P), 서열 번호 192 (O1 PanAsia, R126P), 서열 번호 194 (O1 Manisa 단리물 87 균주, 혈청형 O, R126P), 서열 번호 196 (SAT1-20 iso11, R126P), 서열 번호 198 (SAT2 Egypt 2010, R126P), 및 서열 번호 200 (SAT3 ZIM/6/91, R126P) 중에서 선택되는 변형된 FMDV 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 202 (A Turkey 2006, T100P), 서열 번호 204 (A24 Cruzeiro iso71, T100P), 서열 번호 206 (Asia Lebanon 89, 혈청형 Asia 1, T100P), 서열 번호 208 (Asia1 Shamir, T100P), 서열 번호 210 (C3 Indaial, T100P), 서열 번호 212 (O1 PanAsia, T100P), 서열 번호 214 (O1 Manisa 단리물 87 균주, O 형 혈청형 T100P), 서열 번호 216 (SAT1-20 iso11, T100P), 서열 번호 218 (SAT2 Egypt 2010, T100P), 및 서열 번호 220 (SAT3 ZIM/6/91, T100P) 중에서 선택되는 변형된 FMDV 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 222 (A Turkey 2006, V124P), 서열 번호 224 (A24 Cruzeiro iso71, V124P), 서열 번호 226 (Asia Lebanon 89, 혈청형 Asia 1, V124P), 서열 번호 228 (Asia1 Shamir, V124P), 서열 번호 230 (C3 Indaial, V124P), 서열 번호 232 (O1 PanAsia, V124P), 서열 번호 234 (O1 Manisa 단리물 87 균주, 혈청형 O, V124P), 서열 번호 236 (SAT1-20 iso11, V124P), 서열 번호 238 (SAT2 Egypt 2010, V124P), 및 서열 번호 240 (SAT3 ZIM/6/91, V124P) 중에서 선택되는 변형된 FMDV 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 242 (A Turkey 2006, I22P/L127P), 서열 번호 244 (A24 Cruzeiro iso71, I22P/L127P), 서열 번호 246 (Asia Lebanon 89, 혈청형 Asia 1, I22P/L127P), 서열 번호 248 (Asia1 Shamir, I22P/L127P) 서열 번호 250 (C3 Indaial, I22P/L127P), 서열 번호 252 (O1 PanAsia, I22P/L127P), 서열 번호 254 (O1 Manisa 단리물 87 균주, 혈청형 O, I22P/L127P), 서열 번호 256 (SAT1-20 iso11, I22P/L127P), 서열 번호 258 (SAT2 Egypt 2010, I22P/L127P) 및 서열 번호 260 (SAT3 ZIM/6/91, I22P/L127P) 중에서 선택되는 변형된 FMDV 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 262 (A Turkey 2006, T100P/L127P), 서열 번호 264 (A24 Cruzeiro iso71, T100P/L127P), 서열 번호 266 (Asia Lebanon 89, 혈청형 Asia 1, T100P/L127P), 서열 번호 268 (Asia1 Shamir, T100P/L127P), 서열 번호 270 (C3 Indaial, T100P/L127P), 서열 번호 272 (O1 PanAsia, T100P/L127P), 서열 번호 274 (O1 Manisa 단리물 87 균주, O 형 혈청형, T100P/L127P), 서열 번호 276 (SAT1-20 iso11, T100P/L127P), 서열 번호 278 (SAT2 Egypt 2010, T100P/L127P), 및 서열 번호 280 (SAT3 ZIM/6/91, T100P/L127P) 중에서 선택되는 변형된 FMDV 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 302 (A Turkey 2006, V124P/L127P), 서열 번호 304 (A24 Cruzeiro iso71, V124P/L127P), 서열 번호 306 (Asia Lebanon 89, 혈청형 Asia 1, V124P/L127P), 서열 번호 308 (Asia1 Shamir, V124P/L127P), 서열 번호 310 (C3 Indaial, V124P/L127P), 서열 번호 312 (O1 PanAsia, V124P/L127P), 서열 번호 314 (O1 Manisa 단리물 87 균주, 혈청형 O, V124P/L127P), 서열 번호 316 (SAT1-20 iso11, V124P/L127P), 서열 번호 318 (SAT2 Egypt 2010, V124P/L127P) 및 서열 번호 320 (SAT3 ZIM/6/91, V124P/L127P) 중에서 선택되는 변형된 FMDV 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 322 (A Turkey 2006, I22P/T100P/L127P), 서열 번호 324 (A24 Cruzeiro iso71, I22P/T100P/L127P), 서열 번호 326 (Asia Lebanon 89, 혈청형 Asia 1, I22P/T100P/L127P), 서열 328 (Asia1 Shamir, I22P/T100P/L127P), 서열 번호 330 (C3 Indaial, I22P/T100P/L127P), 서열 번호 332 (O1 PanAsia, I22P/T100P/L127P), 서열 번호 334 (O1 Manisa 단리물 87 균주, 혈청형 O, I22P/T100P/L127P) 서열 번호 336 (SAT1-20 iso11, I22P/T100P/L127P), 서열 번호 338 (SAT2 Egypt 2010, I22P/T100P/L127P), 및 서열 번호 340 (SAT3 ZIM/6/91, I22P/T100P/L127P) 중에서 선택되는 변형된 FMDV 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 342 (A Turkey 2006, I22P/T100P/V124P), 서열 344 (A24 Cruzeiro iso71, I22P/T100P/V124P), 서열 번호 346 (Asia Lebanon 89, 혈청형 Asia 1, I22P/T100P/V124P), 서열 번호 348 (Asia1 Shamir, I22P/T100P/V124P), 서열 번호 350 (C3 Indaial, I22P/T100P/V124P), 서열 번호 352 (O1 PanAsia, I22P/T100P/V124P), 서열 번호 354 (O1 Manisa 단리물 87 균주, 혈청형 O, I22P/T100P/V124P), 서열 번호 356 (SAT1-20 iso11, I22P/T100P/V124P), 서열 번호 358 (SAT2 Egypt 2010, I22P/T100P/V124P), 및 서열 번호 360 (SAT3 ZIM/6/91, I22P/T100P/V124P) 중에서 선택되는 변형된 FMDV 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 412 [소 비염 A (Bovine rhinitis A) Sd-1, C23P], 서열 번호 414 (소 비염 A Sd-1, F120P), 서열 번호 416 (소 비염 A Sd-1, L123P), 서열 번호 418 (소 비염 A Sd-1, T96P), 서열 번호 420 (소 비염 A Sd-1, V22P), 서열 번호 424 (소 비염 B, L124P), 서열 번호 426 (소 비염 B, R23P), 서열 번호 428 (소 비염 B, T97P), 서열 번호 430 (소 비염 B, V121), 및 서열 번호 432 (소 비염 B, V22P) 중에서 선택되는 변형된 소 비염 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 436 (말 비염 A, A125P), 서열 번호 438 (말 비염 A, C23P), 서열 번호 440 (말 비염 A, T127P), 서열 번호 442 (말 비염 A, V100P) 및 서열 번호 446 (말 비염 A, Y22P) 중에서 선택되는 변형된 말 비염 A 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 464 [인간 콕사키바이러스 A10 코왈릭, L102P], 서열 번호 466 (인간 콕사키바이러스 A10 코왈릭, M109P), 서열 번호 468 (인간 콕사키바이러스 A10 코왈릭, N105P), 서열 번호 470 (인간 콕사키바이러스 A10 코왈릭, Q19P), 서열 번호 472 (인간 콕사키바이러스 A10 코왈릭, T106P), 서열 번호 474 (인간 콕사키바이러스 A10 코왈릭, T20P), 서열 번호 476 (인간 콕사키바이러스 A10 코왈릭, T87P), 서열 번호 512 (인간 엔테로바이러스 A71 균주 C4, I104P), 서열 번호 514 (인간 엔테로바이러스 A71 균주 C4, M109P), 서열 번호 516 (인간 엔테로바이러스 A71 균주 C4, N105P), 서열 번호 518 (인간 엔테로바이러스 A71 균주 C4, Q19P), 서열 번호 520 (인간 엔테로바이러스 A71 균주 C4, T106P), 서열 번호 522 (인간 엔테로바이러스 A71 균주 C4, T20P), 및 서열 번호 524 (인간 엔테로바이러스 A71 균주 C4, T87P) 중에서 선택되는 변형된 엔테로바이러스 A 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 480 [인간 콕사키바이러스(coxsackievirus) B1, F109P], 서열 번호 482 (인간 콕사키바이러스 B1, K19P), 서열 번호 484 (인간 콕사키바이러스 B1, L102P), 서열 번호 486 (인간 콕사키바이러스 B1, N105P), 서열 번호 488 (인간 콕사키바이러스 B1, R87P), 서열 번호 490 (인간 콕사키바이러스 B1, T106P), 서열 번호 492 (인간 콕사키바이러스 B1, T20P), 서열 번호 496 (인간 콕사키바이러스 B3 Macocy, F109P), 서열 번호 498 (인간 콕사키바이러스 B3 Macocy, K19P), 서열 번호 500 (인간 콕사키바이러스 B3 Macocy, L102P), 서열 번호 502 (인간 콕사키바이러스 B3 Macocy, N105P), 서열 번호 504 (인간 콕사키바이러스 B3 Macocy, R87P), 서열 번호 506 (인간 콕사키바이러스 B3 Macocy, T106P) 및 서열 번호 508 (인간 콕사키바이러스 B3 Macocy, T20P) 중에서 선택되는 변형된 엔테로바이러스 B 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 448 [인간 콕사키바이러스(coxsackievirus) A1 Tompkins, F109P], 서열 번호 450 (인간 콕사키바이러스 A1 Tompkins, L102P), 서열 번호 452 (인간 콕사키바이러스 A1 Tompkins, N105P), 서열 번호 454 (인간 콕사키바이러스 A1 Tompkins, R87P), 서열 번호 456 (인간 콕사키바이러스 A1 Tompkins, T106P), 서열 번호 458 (인간 콕사키바이러스 A1 Tompkins, T19P), 서열 번호 460 (인간 콕사키바이러스 A1 Tompkins, T20P), 서열 번호 540 (인간 폴리오바이러스 2 균주 R93152, I103P) 서열 번호 542 (인간 폴리오바이러스 2 균주 R93152, L102P), 서열 번호 544 (인간 폴리오바이러스 2 균주 R93152, R87P), 서열 번호 546 (인간 폴리오바이러스 2 균주 R93152, T19P), 서열 번호 548 (인간 폴리오바이러스 2 균주 R93152, T20P), 서열 번호 550 (인간 폴리오바이러스 2 균주 R93152, V104P), 서열 번호 554 (인간 폴리오바이러스 3 단리물 CHN5275/JX/CHN/2001, I103P), 서열 번호 556 (인간 폴리오바이러스 3 단리물 CHN5275/JX/CHN/2001, L102P), 서열 번호 558 (인간 폴리오바이러스 3 단리물 CHN5275/JX CHN5275/JX/CHN/2001, R87P), 서열 번호 560 (인간 폴리오바이러스 3 단리물 CHN5275/JX/CHN/2001, T19P), 서열 번호 562 (인간 폴리오바이러스 3 단리물 CHN5275/JX/CHN/2001, T20P) 서열 번호 564 (인간 폴리오바이러스 3 단리물 CHN5275/JX/CHN/2001, V104P), 서열 번호 568 (인간 폴리오바이러스 Mahony, I103P), 서열 번호 570 (인간 폴리오바이러스 Mahony, L102P), 서열 번호 572 (인간 폴리오바이러스 Mahoney, T10P), 서열 번호 574 (인간 폴리오바이러스 Mahony,T19P), 서열 번호 576 (인간 폴리오바이러스 Mahoney, T20P), 및 서열 번호 578 (인간 폴리오바이러스 Mahoney, V104P) 중에서 선택되는 변형된 엔테로바이러스 C 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 596 (인간 리노바이러스 A20, 균주 VR1130, K88P), 서열 번호 598 (인간 리노바이러스 A20, 균주 VR1130, R87P), 서열 번호 600 (인간 리노바이러스 A20, 균주 VR1130, T19P), 서열 번호 602 (인간 리노바이러스 A20, 균주 VR1130, T20P), 서열 번호 606 (인간 리노바이러스 A89, 균주 VR1199, I114P), 서열 번호 608 (인간 리노바이러스 A89, 균주 VR1199, K88P), 서열 번호 610 (인간 리노바이러스 A89, 균주 VR1199, R87P), 서열 번호 612 (인간 리노바이러스 A89, 균주 VR1199, T112P), 서열 번호 614 (인간 리노바이러스 A89, 균주 VR1199, T19P), 서열 번호 616 (인간 리노바이러스 A89, 균주 VR1199, T20P), 서열 번호 622 (인간 리노바이러스 A2, I114P), 서열 번호 624 (인간 리노바이러스 A2, Q108P), 서열 626 (인간 리노바이러스 A2, R87P), 서열 628 (인간 리노바이러스 A2, R88P), 서열 번호 630 (인간 리노바이러스 A2, T112P), 서열 번호 632 (인간 리노바이러스 A2, T19P), 및 서열 번호 634 (인간 리노바이러스 A2, T20P) 중에서 선택되는 변형된 리노바이러스 A 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 582 (인간 리노바이러스 B14, E114P), 서열 번호 584 (인간 리노바이러스 B14, G88P), 서열 번호 586 (인간 리노바이러스 B14, I112P), 서열 번호 588 (인간 리노바이러스 B14, R87P), 서열 번호 590 (인간 리노바이러스 B14, T19P) 및 서열 번호 592 (인간 리노바이러스 B14, T20P) 중에서 선택되는 변형된 리노바이러스 B 3C 프로테아제를 포함한다.
일부 구현예들에 있어서, 본원에 기술된 단리된 폴리뉴클레오타이드 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의해 코딩된 상기 변형된 피코르나바이러스 3C 프로테아제는 서열 번호 654 (인간 A 형 간염 바이러스, V18P), 서열 번호 652 (인간 A 형 간염 바이러스, G17P), 서열 번호 528 (인간 A 형 간염 바이러스, E20P), 서열 번호 530 (인간 A 형 간염 바이러스, G19P), 서열 번호 532 (인간 A 형 간염 A 바이러스, I130P), 서열 번호 534 (인간 A 형 간염 바이러스, M128P), 및 서열 번호 536 (인간 A 형 간염 바이러스, T100P) 중에서 선택되는 변형된 헤파토바이러스 3C 프로테아제를 포함한다.
추가 변형, 단서
본원에 기술된 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 폴리뉴클레오타이드 및/또는 본원에 기술된 변형된 피코르나바이러스 3C 프로테아제에 추가 변형을 할 수 있다. 예를 들어, 숙주 세포의 형질전환 전에, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 폴리뉴클레오타이드 서열의 코돈 빈도(frequency)는 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 핵산의 발현 또는 안정성의 최적화를 위해 변형될 수 있다. 코돈 사용(usage)을 최적화하는 데 적합한 소프트웨어는 알려져 있고, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 핵산의 코돈 사용(usage)을 최적화하는 데 사용될 수 있다 (genomes._urv.cat/OPTIMIZER/ 에서 이용가능한 Optimizer 참조, 2016.02.05 마지막으로 엑세스됨). 다양한 유기체들에 대한 코돈 사용 빈도는 알려져 있고 at www._kazusa.or.jp/codon/ (2016.02.05 마지막으로 엑세스됨)의 코돈 사용 데이터베이스가 참조로서 또한 본원에 포함된다.
모든 아미노산 코돈이 퇴화(degenerate)되어 있는 것은 아니며, 예를 들어, 대부분의 생물체의 유전 코드에서, Met와 Trp는 단일 코돈으로 코딩된다. 그러나, 퇴화성 코돈의 경우, 코돈 사용의 빈도 또는 평균 빈도는 0% (공통 퇴화 코돈 없음) 내지 100% (숙주 세포 게놈과 동일한 코돈 사용 빈도)의 범위로 선택될 수 있다. 상기 범위는 0%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95% 및 100%를 포함하는 모든 중간 값을 포함한다. 유사하게, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 핵산의 G+C 함량은, 더 많거나 적은 G 또는 C 뉴클레오타이드를 갖는 퇴화 코돈의 선택에 의해 상기 숙주 세포의 것과 매치되거나, 더 가깝게 이동되거나, 더 멀리 이동될 수 있다. 본원의 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 외인성 핵산의 G+C 함량은 숙주 세포의 평균 G+C 함량보다 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50% 초과 또는 미만의 범위일 수 있다.
대안적으로, 코돈 사용은 본원의 변형된 피코르나바이러스 3C 프로테아제의 발현을 조절 또는 제어하고/하거나 숙주 세포의 생존력, 성장 또는 견고성에 요구되는 숙주 세포 단백질의 상기 발현을 약화시키기 위해 변형될 수 있다. 예를 들어, Kew 등의 미국 특허 제8,846,051호가 그 전문이 본원에서 참조로서 포함된다. 일부 구현예들에 있어서, 숙주 세포에 의한 변형된 피코르나바이러스 3C 프로테아제의 발현은 최대 발현률과 비교하여(예: 코돈 빈도가 숙주 세포와 매치되는 경우) 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 95% 또는 그 이상에 의해 제한되거나 감소될 수 있다.
특정 구현들에 있어서, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩되는 변형된 FMDV 3C 프로테아제의 위치 126 (R126)의 아르기닌 잔기는 치환되지 않는 것이 바람직하다. 비제한적 예로서, 본원에서 제공된 변형된 FMDV 3C 프로테아제는, 아스파르트 산 및 글루탐산과 같은 산성 아미노산에 의한 R126의 치환을 포함하지 않는다. 더욱 특별하게, 상기 변형된 FMDV 3C 프로테아제는 R126의 글루탐산에 의한 치환(즉, R126E)을 포함하지 않는다.
특정 구현들에 있어서, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩되는 변형된 피코르나바이러스의 변형된 FMDV 3C 프로테아제의 위치 42 (L42)의 류신 잔기는 히스티딘으로 치환되는 것이 바람직하다. 상기 변형된 FMDV 3C 프로테아제는 본원에 기술된 임의의 혈청형으로부터 유도되거나 수득될 수 있다. 위치 42에 히스티딘을 도입한, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩되는 예시적인 변형된 FMDV 3C 프로테아제는, 서열 번호 158, 160, 162, 164, 166, 168, 170, 172, 174 및 176에 의해 기재되어 있다. 서열 번호 158, 160, 162, 164, 166, 168, 170, 172, 174 및 176은 각각 서열 번호 157, 159, 161, 163, 165, 167, 169, 171, 173 및 175에 의해 코딩된다.
특정 구현들에 있어서, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩되는 변형된 FMDV 3C 프로테아제에서 위치 34의 트레오닌 잔기는 알라닌으로 치환되고, 위치 88의 메티오닌은 류신으로 치환되는 것이 바람직하다. 상기 변형된 FMDV 3C 프로테아제는 본원에 기술된 임의의 혈청형으로부터 유도되거나 수득될 수 있다. 각각 위치 34 및 88에서 알라닌 및 류신을 각각 포함하는, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩되는 예시적인 변형된 FMDV 3C 프로테아제는 서열 번호 282, 284, 286, 288, 290, 292, 294, 296, 298 및 300으로 기재되고, 이것들은 서열 번호 281, 283, 285, 287, 289, 291, 293, 295, 297 및 299로 각각 코딩된다.
형질전환 유전자 발현 카세트
본원의 다른 측면은 본원의 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드를 포함하는 단리된 폴리뉴클레오타이드에 관한 것이다. 일부 구현예들에 있어서, 상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 상기 단리된 폴리뉴클레오타이드를 포함하는 상기 단리된 폴리뉴클레오타이드는 형질전환 유전자 발현 카세트이다. 일부 구현예들에 있어서, 상기 형질전환 유전자 발현 카세트는 피코르나바이러스 P1 폴리펩타이드 전구체를 코딩하는 뉴클레오타이드 서열을 추가 포함한다. 일부 구현예들에 있어서, 상기 P1 폴리펩타이드 전구체를 코딩하는 상기 뉴클레오타이드 서열은 A, O, C, Asia 1, SAT1, SAT2 및 SAT3 혈청형, 및 이들 7 가지 혈청형의 아형, 지역형 및 균주들 또는 다른 피코르나바이러스 단리물 또는 변이체의 임의의 것으로 수득될 수 있거나, 유래될 수 있다.
일부 구현예들에 있어서, FMDV P1 폴리펩타이드 전구체와 같은, 피코르나바이러스 P1 폴리펩타이드 전구체를 코딩하는 뉴클레오타이드 서열은 서열 번호 362 (A Turkey 2006, 야생형), 서열 번호 365 (서열 번호 A24 Cruzeiro iso71, 야생형), 서열 번호 368 (Asia1 Shamir, 야생형), 서열 번호 371 (C3 Indaial, 야생형), 서열 번호 374 (O1 Manisa 단리물 87 균주, 혈청형 O, 야생형), 서열 번호 377 (O1 PanAsia, 야생형), 서열 번호 380 (SAT1-20 iso11, 야생형), 및 서열 번호 383 (SAT2 Egypt 2010, 야생형), 서열 번호 386 (SAT3 ZIM/6/91, 야생형) 중에서부터 선택된다.
상기 바로 위의 뉴클레오타이드 서열에 의해 코딩된 상기 P1 폴리펩타이드의 상기 아미노산 서열들은 각각, 서열 번호 363, 366, 369, 372, 375, 378, 381, 384 및 387에 의해 기재된 것을 포함한다. P1 폴리펩타이드는, 예를 들어, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 또는 그 이상의 아미노산 잔기들의 결실, 치환 또는 삽입에 의해 변형되며, 단, 상기 P1 폴리펩타이드는 동물의 면역계 (예를 들어, FMDV 포유동물 숙주)에 의해 인식된, 체액성 및 세포성 에피토프 모두를 포함하여, 하나 이상의 에피토프를 유지하거나 포함한다. 천연 P1 폴리펩타이드는 적어도 62%-100% 동족성(homologous)이다. 일부 구현예들에 있어서, 본원에 따른 P1 폴리펩타이드는, FMDV 캡시드와 같은, 피코르나바이러스 캡시드로 가공 및 조립될 수 있는 것으로 기재된다. P1 폴리펩타이드는 본원에 기술된 임의의 상기 P1 폴리펩타이드 또는 다른 공지의 알려진 P1 폴리펩타이드와 60, 70, 80, 90, 95, 96, 97, 98, 99 또는 100% (또는 임의의 중간 값) 상동성 또는 유사성을 갖는 것으로서 구조적으로 기술될 수 있다.
다른 구현예들에 있어서, FMDV P1 폴리펩타이드 전구체와 같은, 피코르나바이러스 P1 폴리펩타이드 전구체를 코딩하는 상기 폴리뉴클레오타이드 서열은 내부 제한 부위들을 제거하기 위해 변형된다. 이러한 FMDV P1 폴리펩타이드 전구체는 서열 번호 361 (A Turkey 2006, 제한 부위 제거됨), 서열 번호 364 (A24 Cruzeiro iso71, 제한 부위 제거됨), 서열 번호 367 (Asia1 Shamir, 제한 부위 제거됨), 서열 번호 370 (C3 Indaial, 제한 부위들이 제거됨), 서열 번호 373 (O1 Manisa 단리물 87 균주, 혈청형 O), 서열 번호 376 (O1 PanAsia, 제한 부위들이 제거됨), 서열 번호 379 (SAT1-20 iso11, 제한 부위들이 제거됨), 서열 번호 381 (SAT2 Egypt 2010, 제한 부위 제거됨), 및 서열 번호 385 (SAT3 ZIM/6/91, 제한 부위들이 제거됨) 중에서 선택될 수 있다.
본원의 다른 구현예에 있어서, 본원에 기술된 돌연변이 전략 및 기술들은 상기 P1 전구체를 코딩하는 상기 뉴클레오타이드 서열에 하나 이상의 돌연변이들을 도입하여, 최종 조립된 캡시드 생성물의 안정성을 향상하기 위해 적용될 수 있다. 도입될 수 있는 상기 돌연변이들 중, 논센스 돌연변이들을 포함할 수 있으며, 상기 논센스 돌연변이들은 임의의 아미노산 치환을 야기하지 않음으로써 복제 및 서브-클로닝을 용이하지만 동일한 변역된 단백질 생산물을 유지하도록 제한 효소 인식 부위를 효과적으로 제거한다. 상기 돌연변이들은 개별적인 발병의 필요에 즉시 응답하기 위해, 예를 들어, FMDV 혈청형과 상이한 다른 P1 폴리펩타이드 전구체들을 교환하기 위한 형질전환 유전자 발현 카세트에 상기 P1 폴리펩타이드 전구체의 클로닝-in 및 클로닝-out을 향상시킨다.
추가 구현예에 있어서, 본원의 상기 형질전환 유전자 발현 카세트는 발현 벡터에 복제하기 위한 상기 발현 카세트의 N-말단 및 C-말단의 각각에서 제한 효소 인식 부위 또는 서열들을 추가 포함한다. 이러한 제한 효소 인식 부위의 예로는 EcoRI, EcoRII, BamHI, HindIII, TaqI, NotI, HinFI, Sau3AI, PvuII, SmaI, NheI, HaeIII, HgaI, AluI, EcoRV, KpnI, PstI, SacI, SpeI, StuI, SphI, XbaI, SalI, ScaI, XcmI, BsiWI, XhoI, BstEII, PflMI, AccI, SacII, PpuMI, AgeI, NcoI, BstXI, MluI 및 AatI에 대한 인식 서열들을 포함하나, 이에 제한되는 것은 아니다. 이러한 형질전환 유전자 구조체는 벡터 또는 폴리뉴클레오타이드 구조체에 복제될 수 있고 숙주 세포에 형질주입 될 수 있다.
일부 구현예들에 있어서, 본원에 기술된 구현예들에 따른 상기 형질전환 유전자 발현 카세트는, 완전한 피코르나바이러스 게놈을 코딩하지 않아 상기 형질전환 유전자 발현 카세트를 포함하는 백신의 제조 또는 투여 동안 우연적인 발병, 예를 들어, 구제역 바이러스 발생이 일어날 수 없다.
또한, 전형적으로, 상기 형질전환 유전자 발현 카세트는, 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 FMDV 3C 프로테아제와 같은, 예를 들어, FMDV 비-구조 단백질인 하나의 피코르나바이러스 비-구조 단백질만을 코딩한다. 전형적으로, 상기 형질전환 유전자 발현 카세트를 포함하는 백신으로 치료된 동물들은 자연적 피코르나바이러스 감염 동안 발현되는 엔테로바이러스(Enterovirus)와 같은, 다른 피코르나바이러스, 비-구조 단백질에 대한 항체들을 생성하지 않을 것이다. 예를 들어, 상기 형질전환 유전자 발현 카세트가 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 FMDV 3C 프로테아제를 코딩하는 돌연변이 뉴클레오타이드 서열을 포함하는 경우, 상기 변형된 FMDV 3C 프로테아제에 대한 항체만을 생성하고, 2B, 2C, 3B, 3D 등과 같은 다른 비-구조 단백질들에 대한 항체를 생성하지 않는다 (도 1 참조). 상기 형질전환 유전자 발현 카세트에 의한 백신 접종과 비교하여, 자연적 감염 후에 생성된 항체 프로파일의 차이는, 백신 접종된 동물들로부터 감염된 동물의 구별과 그 반대의 경우를 구별하는 능력을 부여한다.
일부 구현예들에 있어서, 본원에 따른 상기 형질전환 유전자 발현 카세트는 단일 개방 판독 프레임으로서 구축된다. 상기 P1 폴리펩타이드 전구체를 코딩하는 상기 뉴클레오타이드 서열은, 변형된 FMDV 3C 프로테아제와 같이, 변형된 피코르나바이러스를 코딩하는 상기 뉴클레오타이드 서열에 대하여 5' 또는 3'에 위치될 수 있다.
특정 구현예들에 있어서, 상기 형질전환 유전자 발현 카세트는 P1 폴리펩타이드 전구체를 코딩하는 상기 뉴클레오타이드 서열과, 변형된 FMDV 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 상기 돌연변이 뉴클레오타이드 서열 사이에 위치된 변역 조절 요소를 추가 포함하여, 단일 개방 판독 프레임에서 상기 두 개의 단백질의 개별, 동일 몰량의 동등한 발현을 유익하게 가능하게 한다.
일부 구현예들에 있어서, 상기 번역 조절 요소는 5 개 내지 50 개 아미노산 잔기 길이, 전형적으로 15 개 내지 40 개 잔기들, 더 전형적으로 25 개 내지 35 개 잔기들의 번역 인터럽터 서열이다. 추가 구현예들에 있어서, 상기 번역 인터럽터 서열은 하나 이상의 번형된 피코르나바이러스 3C 프로테아제(들) 비-구조 단백질 (예: 1A, 1B, 1C, 1D, 2A, 2B, 2C, 3A, 3B, 3C, 3D)의 일부를 포함할 수 있다. 일부 구현예들에 있어서, 상기 번역 인터럽터 서열은 상기 18-아미노산 FMDV 2A 단백질에 상기 1D FMDV 단백질의 C-말단의 11-아미노산 서열을 도입하고 FMDV 2A 단백질의 C-말단에 프롤린 잔기를 도입함으로써 형성된다.
일부 구현예들에 있어서, 상기 형질전환 유전자 발현 카세트는 Kozak 컨센서스 서열과 같은, 진핵생물에서 번역의 개시를 위한 뉴클레오타이드 서열을 추가 포함한다. 일부 구현예들에 있어서, 상기 P1 폴리뉴클레오타이드 전구체를 코딩하는 상기 뉴클레오타이드 서열은, 변형된 FMDV 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 돌연변이 뉴클레오타이드 서열에 대하여 5'에 위치되고, 상기 진핵생물 번역 개시 서열은 상기 P1 폴리펩타이드 전구체를 코딩하는 상기 뉴클레오타이드 서열에 대하여 업스트림(upstream) 또는 5'에 위치된다. 대안적인 구현예에 있어서, 변형된 FMDV 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 상기 돌연변이 뉴클레오타이드 서열은 상기 P1 폴리펩타이드 전구체를 코딩하는 뉴클레오타이드 서열에 대하여 5'에 위치되고, 상기 진핵 생물 번역 개시 서열은 변형된 FMDV 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 돌연변이 뉴클레오타이드 서열에 대하여 업스트림 또는 5'에 위치된다.
추가 구현예들에 있어서, 상기 형질전환 유전자 발현 카세트는 프로모터를 포함한다. 특정 구현예들에 있어서 상기 진핵생물 번역 개시 서열과 같이, 상기 프로모터는, 상기 P1 폴리펩타이드 전구체 및 변형된 FMDV 3C 프로테아제와 같은 변형된 피코르나바이러스 3C를 코딩하는 두 개의 뉴클레오타이드 서열의 정렬에 의존하여, 상기 P1 폴리펩타이드 전구체를 코딩하는 뉴클레오타이드 서열 또는 돌연변이 FMDV 3C 프로테아제를 코딩하는 상기 돌연변이체 뉴클레오타이드에 대하여, 업스트림 또는 5'에 위치된다. 특정 구현예들에 있어서, SV40, CMV, UBC, EF1A, PGK 및 CAG를 비제한적으로 포함하는 강하고 구조적인(constitutive) 프로모터는 강한 면역 응답을 유도하기 위해 포유동물 숙주에서 형질전환 유전자 발현의 연장된 높은 수준을 위하여 본원의 상기 형질전환 유전자 발현 카세트가 유리하게 도입될 수 있다. 정지 코돈 서열 (예: TAA, TGA 및 TAG)은 mRNA 번역의 중단을 확실히 하기 위해 본원의 상기 형질전환 유전자 발현 카세트의 말단에 선택적으로 첨가될 수 있다.
벡터(Vectors)
본원의 다른 측면은 본원에 기술된 상기 형질전환 유전자 발현 카세트를 포함하는 벡터들에 관한 것이다. 전형적으로, 상기 형질전환 유전자 발현 카세트는 포유동물 발현 벡터 시스템에 복제될 수 있다. 일부 구현예들에 있어서, 상기 형질전환 유전자 발현 카세트는 pSNAP 벡터 (New England Biolabs)에 복제될 수 있다. 본원의 상기 변형된 FMDV 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드를 포함하는 pSNAP 벡터들의 예들은 서열 번호 406 내지 408 및 410에서 기재된다. 추가 구현예들에 있어서, 상기 pSNAP 벡터의 변형은 전체 벡터 크기의 감소 및/또는 다중 복제 부위에서 하나 이상의 제한 효소 인식 서열의 제거를 포함하나, 이에 제한되는 것은 아니다.
다른 구현예들에 있어서, 상기 형질전환 유전자 발현 카세트를 전달하기 위해 사용되는 상기 벡터는 미니서클(minicircle) DNA 벡터이다. 전형적으로, 미니서클 DNA 벡터는 형질전환 유전자 발현 카세트를 담지하는 미니서클이고, 삽입이 없는 빈(empty) 벡터를 포함하지 않는다.
형질전환 유전자 발현 카세트를 담지하고 전달하기 위한 미니서클 DNA 벡터의 사용은 포유동물 세포가 중간 진핵생물 숙주 세포 시스템 (예: 곤충 세포주 생산 시스템)을 이용하지 않고 형질주입(예: 직접적으로)되도록 한다. 포유동물 세포를 형질전환 유전자 발현 카세트를 담지한 미니서클 DNA 벡터에 직접 형질주입하는 것은 생산 설비에서 대량 부피의 중간 숙주 세포 배양을 유지하고 중간 숙주 세포에 의해 생성된 빈 캡시드 또는 바이러스-유사 입자 (VLPs)를 수확하는 것과 연관된 비용 및 노동을 제거할 수 있다
또한, 미니서클 벡터들은 전형적으로 표준 플라스미드 벡터보다 더 작고 외인성 박테리아 서열들이 결여되어 있다. 일부 구현예들에 있어서, 이러한 특징들은 세포들의 형질주입을 향상시키고 포유동물 숙주 세포 내에서 형질전환 유전자 발현의 연장된 지속을 가능하게 한다. 예를 들어, 미니서클 벡터는 플라스미드에서 발견되는 외인성 박테리아 서열이 결여되기 때문에 표준 벡터보다 더 작다. 플라스미드 벡터들과 미니서클 벡터 사이의 크기의 차이는 외인성 박테리아 서열의 결여, 상기 플라스미드 및 이들의 변이체와 비교하여 현저히 더 작은 것과 같이, 비외인성 박테리아 서열들을 실질적으로 포함하지 않는 것에 기인될 수 있다.
포유동물 숙주 세포에서 피코르나바이러스 바이러스-유사 입자의 생성을 유도 할 수 있는 미니서클 벡터들을 생성하는 방법은 전형적으로 2-단계 과정을 포함한다. 첫째, 박테리아 서열 및 형질전환 유전자를 포함하는 전체-크기 부모 플라스미드가, 예를 들어, 대장균에서 생성된다. 상기 부모 플라스미드가 여전히 상기 대장균 숙주 내부에 있는 동안, 부위-특이적 재조합효소(recombinase)의 발현이 유도되고 상기 원핵생물 또는 박테리아 본(bone)이 상기 재조합효소 인식 부위에서 상기 효소에 의해 절단된다. 부위-특이적 재조합효소의 비제한적인 예는 Cre 재조합효소, Flp 재조합효소, ParA 절단효소(resolvase) 및 PhiC31 인테그라아제(integrase)와 같은 Tyr- 및 Ser- 재조합효소가 있다. 적합한 재료, 기술, 접근법 및 방법의 그 전문이 본원에 참조로 포함되는, 미국 특허 제8,236,548호에 기재되어 있다. 추가 설명은 Kay 등, A Robust System for Production of Minicircle DNA Vectors, Nature Biotechnology 28 1287-1289 (2010)에서 찾을 수 있고, 이것의 전문은 본원에 참조로 포함된다.
형질전환된 숙주 세포
본원의 다른 측면은 적어도 피코르나바이러스 P1 폴리펩타이드 전구체 및 변형된 피코르나바이러스 3C 프로테아제, 예를 들어, FMDV 3C 프로테아제를 발현하는 형질전환 유전자 발현 카세트를 담지하는 벡터로 형질전환 유전자되거나 형질주입된 세포에 관한 것으로서, 이것은, 대조군 3C 프로테아제 및 적어도 피코르나바이러스 P1 폴리펩타이드 전구체로 형질전환된 숙주 세포와 비교하여, 상기 형질전환된 또는 형질주입된 숙주 세포에 대한 독성이 감소되지 않으며, FMDV P1 폴리펩타이드 전구체와 같은 피코르나바이러스 P1 폴리펩타이드 전구체를 VP1, VP2, VP3 및 VP4 또는 VP0, VP1 및 VP3의 FMDV 캡시드 단백질과 같은, 개별 피코르나바이러스 캡시드 단백질로 전체적으로 가공할 수 있다. 상기 숙주 세포들은 박테리아 세포와 같은, 또는 원핵생물, 또는 진핵생물일 수 있다. 전형적으로, 상기 숙주 세포는, 인간 배아 신장 세포주 (HEK-293) 또는 연속적인 돼지 세포주 LF-BK αV/β6와 같은, 진핵생물, 동물 세포 (특히 포유동물 세포), 식물 세포 및 효모 세포일 수 있다. 구체적인 적합한 숙주 세포는 본원에서 "요약" 표제 하에 기술된 것들을 포함하여 기재되어 있다.
상기 숙주 세포들은 통상의 기술자에게 잘 알려져 있고 본원에 기재된 바와 같은 임의의 통상적인 형질전환 유전자 기술을 사용하여 형질전환될 수 있다. 본원의 상기 숙주 세포는, 조절된 조건 하에서, 본원의 형질전환 유전자 카세트 및/또는 재조합 벡터들로 형질주입되기 전 및/또는 후, 세포 배양과 같은, 그것들의 자연 환경의 외부에서 일반적으로 성장될 수 있다. 다른 구체예들에 있어서, 상기 숙주 세포는 그것들의 자연 환경의 내부에서, 예를 들어, 유기체의 일부로서 성장된다.
조성물
본원의 다른 측면들은, 본원의 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드, 형질전환 유전자 발현 카세트 및/또는 재조합 벡터, 및 약학적으로 허용가능하고 본원의 변형된 피코르나바이러스 3C 프로테아제에 의한 단백질 분해에 적합한, 부형제, 보조제, 완충액 또는 용액을 포함하는 조성물에 관한 것이다.
일부 구현예들에 있어서, 본원의 상기 조성물은 백신과 같은 면역원성 조성물, 전형적으로 DNA 백신이다. 본원에 사용된 것과 같이, 용어 "면역원" 또는 "면역원성(immunogenic)"은 숙주에서 면역 반응, 예를 들어, 항체 반응을 유도하는 임의의 기질을 의미한다. 본원에 기재된 면역원성 조성물은 면역보호성 또는 치료성일 수도 있고 또는 아닐 수도 있다. 따라서, 용어 "면역원성"은 백신에만 국한되지 않는다.
용어 "면역 반응 유도"는, 항원과 같은 자극에 반응하는 생체 내 (in vivo)에 면역 세포의 자극을 의미한다. 상기 면역 반응은, 예를 들어, T 세포 및 대식세포 자극과 같은, 세포성 면역 반응, 및, 예를 들어, B 세포 및 보체 자극과 같은 체액성 면역 반응, 및 항체 생성 중 하나 또는 둘을 포함할 수 있다. 면역 반응은, 예를 들어, 항체 면역 분석법, 증식 분석법 및 기타를 포함하여, 본 기술분야에 공지된 기술을 사용하여 측정될 수 있다.
일부 구현예들에 있어서, 본원에 기술된 상기 면역원성 조성물은 항체 조성물을 수득하는데 사용될 수 있고, 이어서 일시적인 면역, 즉 인공적으로 수득된 수동 면역을 제공하기 위하여 대상(subject)에게 투여될 수 있다. 상기 항체 조성물들을 제조하고 투여하는 방법은 본 기술분야에 공지되어 있고, 예를 들어, 본원에 참조로 그 전문이 포함된 미국 특허 제4,748,018호에 기재되어 있다.
일부 구현예들에 있어서, 본원의 상기 면역원성 조성물은 백신이다. 본원에 사용된 용어 "백신"은 항원을 포함하는 조성물을 의미하고, 이것은 상기 대상에서 바이러스에 대한 면역성을 확립하는데 유용하다. 백신은 예방적 또는 치료적일 수 있다. 예를 들어, 백신은 대상에 대한 바이러스의 영향을 예방, 완화, 경감 또는 제거 할 수 있다.
일부 구현예들에 있어서, 본원의 상기 면역원성 조성물은, FMDV P1 폴리펩타이드 전구체와 같은, 적어도 피코르나바이러스 P1 폴리펩타이드 전구체, 및 변형된 FMDV 3C 프로테아제와 같은, 변형된 피코르나바이러스 3C 프로테아제를 발현하는 형질전환 유전자 발현 카세트를 담지하는 면역원성 양의 하나 이상의 벡터를 포함할 수 있으며, 이것은 대조군 피코르나바이러스 3C 프로테아제, 및 적어도 피코르나바이러스 P1 폴리펩타이드 전구체로 형질전환된 또는 형질주입된 숙주 세포와 비교하여, 형질전환된 또는 형질주입된 숙주 세포에 대한 감소된 독성을 가지는 VP1, VP2, VP3 및 VP4 또는 VP0, VP1 및 VP3의 개별 피코르나바이러스 캡시드 단백질로 상기 피코르나바이러스 P1 폴리펩타이드 전구체를 전체적으로 가공할 수 있다. "면역원성 양"은 피코르나바이러스 (예: FMDV)에 대한 면역 반응을 유도할 수 있는 양이다.
하나 이상의 구현예들에 있어서, 본원의 상기 조성물, 백신과 같은 면역원성 조성물은 임의의 알려진 방법에 의해 제제화될 수 있다. 상기 조성물들은, 예를 들어, 주사제 (예 : 피하, 피내 및 근육 내 주사, 제트 주사), 경구 투여용 제제, 비내 투여 (예 : 에어로졸 흡입 또는 점적), 눈 국소 투여, 전기천공, 유전자 총, 형질주입, 리포좀 매개 전달 또는 이들의 조합물로서 액체 용액 또는 현탁액으로서 제제화될 수 있다. 주사 또는 다른 투여 전에 액체의 용액 또는 현탁액에 적합한 고체 형태로도 제조될 수 있다. 상기 제제는 또한 유화되거나 또는 리포좀에 캡슐화될 수 있다.
하나 이상의 구현예들에 있어서, 본원의 상기 백신은 동일한 종 또는 상이한 종의 2 종 이상의 상이한 균주에 대한 면역 반응을 자극하는 면역원성 조성물들을 포함하는 다중(multivalent) 또는 다가(polyvalent) 백신으로서 제제화될 수 있다. 추가 구현예들에 있어서, 본원의 다중 백신은 본원의 변형된 피코르나바이러스 3C 프로테아제 또는 3CD 융합체 또는 본원의 재조합 벡터를 사용하여 P1 전구체로부터 가공된 VLPs로부터 형성된 적어도 하나의 피코르나바이러스 캡시드를 포함한다. 또 다른 구현예들에 있어서, 상기 다중 백신은 살아 있는, 죽은, 약화 또는 불활성화된 완전 미생물; 그의 독소(toxoids), 그의 서브유닛; 그의 VLPs; 그의 컨쥬게이트; 또는 그의 핵산을 포함할 수 있으나, 이에 제한되는 것은 아니다.
하나 이상의 구현예들에 있어서, 상기 활성 면역원성 성분들, 예를 들어, 변형된 3C 프로테아제를 코딩하는 폴리뉴클레오타이드, 또는 3CD 융합체, 및 P1 전구체 폴리펩타이드를 포함하는 벡터를 보조제, 염, 담체, 부형제 또는 희석제와 혼합하며, 이것들은 약학적으로 허용가능하고 상기 활성 성분과 상용성이다.
일부 구현예들에 있어서, 보조제들은 백신과 같은 면역원성 조성물에 첨가되어 더 많은 양의 항체 및 더 오래 지속되는 보호를 제공하는 것과 같이 상기 면역 반응을 증가시킴으로써 변형하고, 이에 따라 주입된 이물질의 양을 최소화한다. 보조제들은, 예를 들어, 항체를 분비하는 B 세포 대신에 상기 T 세포를 활성화시키는 것에 의해 면역계의 특정 세포 유형에 대한 상기 면역 반응을 파괴하는 것을 도움으로써 백신의 효능을 향상 시키는데 또한 사용될 수 있다. 보조제들의 예로는, 수성-기반 알루미늄 수산화물 겔 사포닌, 오일-기반의 Montanide ISA 206, 다른 알루미늄-기반 보조제, 불완전 Freunds 보조제 (IFA), 및 파라핀 오일을 포함할 수 있으나, 이에 제한되는 것은 아니다.
추가 구현예에 있어서, 약학적으로 허용가능한 염은 무기산 (예 : 염산 또는 인산) 및 유기산 (예 : 아세트산, 옥살산, 타르타르산 또는 말레산)으로 형성된 산 부가 염들(펩타이드의 유리 아미노기로 형성됨)을 포함할 수 있으나, 이에 제한되는 것은 아니다. 유리 카르복실기로 형성된 염은 무기 염기 (예 : 수산화나트륨, 수산화칼륨, 수산화암모늄, 수산화칼슘 또는 수산화 제 2 철) 및 유기 염기 (예 : 이소프로필아민, 트리메틸아민, 2-에틸아미노-에탄올, 히스티딘 및 프로카인)로부터 또한 유도될 수 있다.
추가 구현예에 있어서, 적합한 담체는 액체 담체 (예를 들어, 물, 식염수, 배양 배지, 생리 식염수, 수성 덱스트로스, 수성 글리콜) 및 고체 담체 (예 : 전분, 글루코오스, 락토오스, 수크로오스, 덱스트란과 같은 탄수화물; 아스코빅산 및 글루타티온과 같은 항산화제, 가수분해된 단백질)을 포함할 수 있으나, 이에 제한되는 것은 아니다.
추가 구현예에 있어서, 적합한 부형제는 물, 식염수, 덱스트로스, 글리세롤, 에탄올 등, 이들의 조합을 포함하나, 이에 제한되는 것은 아니다.
추가의 구현예에 있어서, 희석제들의 예는 물, 생리 식염수, 인간 혈청 알부민, 오일, 폴리에틸렌 글리콜, 글리세린, 프로필렌 글리콜 또는 다른 합성 용매, 벤질 알코올과 같은 항균제, 아스코빅산 또는 이황산나트륨과 같은 항산화제, 에틸렌 디아민-테트라-아세트산과 같은 킬레이트제, 아세테이트, 시트레이트염 또는 포스페이트염과 같은 완충제, 및 염화나트륨 또는 덱스트로스와 같은 삼투압 조절제를 포함할 수 있으나, 이에 제한되는 것은 아니다.
추가 구현예에 있어서, 상기 백신은 습윤제 또는 유화제, pH 완충제 및/또는 다른 제제와 같은 소량의 보조 물질들을 포함할 수 있고, 이것은 상기 백신의 유효성을 향상시킨다. 효과적인 제제의 예는 하기를 포함하나, 이에 제한되는 것은 아니다: 수산화알루미늄; N-아세틸-무라밀-L-트레오닐-D-이소글루타민 (thr-MDP); N-아세틸-노르-무라밀-L-알라닐-D-이소글루타민 (CGP 11637, 노르-MDP로 지칭됨); N-아세틸-무라밀-L-알라닐-D-이소글루타민 1-L-알라닌-2 (1'-2'- 디팔미토일-sn-글리세로-3-히드록시포스포릴옥시)-에틸 아민(CGP 19835A, MTP-PE로 지칭됨); 및 박테리아에서 추출된 세 가지 성분을 포함하는 RIBI; 2% 스쿠알렌/트윈 80 유제에서 모노포스포릴 지질 A, 트레할로스 디미코레이트 및 세포벽 골격 (MPL+TDM+DWS). 상기 보조제의 유효성은 문제의 보조제를 포함하는 백신에서 상기 면역원의 투여로 인한 면역원에 대한 항체 (특히 IgG, IgM 또는 19A)의 양을 측정함으로써 결정될 수 있다. 추가 구현예에 있어서, 추가 제제들 및 투여 방식이 또한 사용될 수 있다.
하나 이상의 구현예들에 있어서, FMDV 백신과 같은 본원에 따른 상기 피코르나바이러스 백신은 자연 감염과는 다른 면역 반응을 유도하는, 마커 백신 또는 DIVA (Differentiating Infected from Vaccinated Animals)이다. 이러한 차이는 항체 프로파일에 반영되며, 상기 백신 제제에 사용된 것과 동일한 조성물을 포함하는 효소-연결 면역흡착 분석법 (ELISA)과 같은 진단 테스트 및 분석에 의해 검출될 수 있다. DIVA 전략은 FMDV 백신과 같은 DIVA 피코르나바이러스 백신을 사용한 응급 접종이 집단 밀도가 높은 축산 지역에서의 FMDV 발병과 같은 피코르나바이러스 발병에 효과적인 제어 도구가 될 수 있는 박멸 시나리오에서 유용하다. DIVA 백신 접종은 FMDV 박멸과 같은 피코르나바이러스 박멸 과정에서 추방된 동물 수를 제한할 수 있어, 질병 조절 조치에 대한 대중의 수용을 향상하고 및 경제적 손실을 제한할 수 있다.
피코르나바이러스 DNA 백신의 효능은 12 개월 동안, 백신 접종을 받지 않은 대상 집단의 발생률과 비교하여, 백신 접종된 대상 집단 중에서 피코르나바이러스의 발생률의 감소율에 의해 결정될 수 있다. 백신 유효성은 하기 식을 사용하여 측정할 수 있다: VE = [(ARU - ARV)/ARU] x 100%, 여기서 "VE"는 백신 유효성, "ARU"는 백신 접종을 하지 않은 집단의 발생률, "ARV"는 백신 접종한 집단 발생률.
일부 구현예들에 있어서, 본원에 따른 미니서클 DNA 벡터를 포함하는 FMDV 백신과 같은 피코르나바이러스 DNA 백신은 50-95%, 약 50%, 50% 초과, 50%, 약 75%, 75% 초과, 약 90%, 90% 초과, 95%, 약 95% 또는 95% 초과 사이의 VE 값들을 나타낸다.
특정 구현예들에 있어서, 면역원성 조성물 및 백신을 포함하는 본원의 상기 조성물은, 숙주 세포 배양물, 그의 단편 (들) 또는 서브유닛(들)로부터 단리되고 및 정제된 하나 이상의 피코르나바이러스 VLPs의 면역원성 양을 포함한다. 일부 구현예들에 있어서, 상기 VLP는 본원에서 제공되는 상기 변형된 3C 프로테아제를 사용하여 수득된다. 다른 구현예들에 있어서, 상기 백신은 하나 이상의 피코르니아 바이러스 캡시드 단백질 및/또는 그의 일부를, 피코르나바이러스 VLPs의 표면 상의 보조제 분자 및 그의 일부와 조합하여 포함할 수 있고, 선택적으로, 하나 이상의 바이러스 입자 또는 그로부터 유래된 에피토프 펩타이드와 자연적으로 연관된 하나 이상의 피코르나바이러스 바이러스 성분과 같은 다른 단백질 또는 다른 면역원을 추가 포함할 수 있다.
방법
본원의 다른 측면은, 유전적으로 조작된 DNA (예를 들어, 형질전환 유전자 발현 카세트+발현 벡터)를 포유동물 대상에게 투여함으로써 FMDV와 같은 피코르나바이러스의 하나 이상의 균주에 대해 포유동물 대상을 보호하는 것에 관한 것이고, 상기 포유동물 대상에서 FMDV VLPs와 같은 피코르나바이러스 VLPs의 조립을 통한 면역 반응을 in situ 생성한다. 적합한 포유동물 대상들은, 들소 (bison)와 같은 가축 소 또는 야생 소(wild bovids), 아프리카 버팔로, 영양, 임팔라, 가젤, 양, 염소 및 가축동물을 포함하는, 인간, 영장류 및/또는 갈라진-발굽 동물을 포함하는 임의의 포유동물 대상을 포함할 수 있다.
전통적인 전병원체(whole-pathogen) 백신 및 단백질-기반 백신과 비교하여, DNA 백신 플랫폼과 연관된 많은 이점이 있다. 예를 들어, DNA 백신은 죽었거나 살아 있는 실제 감염원을 포함하지 않는다. DNA 백신들은 또한 장기 저장 및 운송을 위해 쉽게 동결 건조될 수 있고, 콜드 체인(cold chain) 전달을 요구하지 않는다.
또한, DNA 백신의 상기 DNA 벡터는, 종래 백신 제제와 비교하여, 더 빠르고 더 쉽게 생산 및 변형될 수 있다. 이것은, 특정 FMDV 발생과 같은, 개별 피코르나바이러스에 맞추어 DNA 백신을 특이적으로 조작하기 위한 더 신속한 응답을 가능하게 한다. 일부 구현예들에 있어서, 상기 형질변환 유전자 발현 카세트를 담지 및 전달하기 위해 미니서클 DNA 벡터를 사용하는 것은, 중간 진핵생물 숙주 세포 시스템의 사용을 제거하고, 상기 FMDV 발생의 진행과 같은, 발병 동안 중간 숙주 세포 시스템의 변형을 포함하여, 연관된 비용 및 노동을 제거한다.
하나 이상의 구현예들에 있어서, FMDV 백신과 같은 피코르나바이러스 백신은, 복용 제제와 상용할 수 있는 방식으로, 그리고 예방적으로 및/또는 치료학적으로 유효할 수 있는 양 및 방식으로, 예방적으로 (예: 미래 감염의 효과를 예방 또는 개선하기 위해), 치료학적으로 (예: 감염된 유기체의 상기 면역계를 치료하거나 강화하기 위해), 또는 둘 다를 위해 투여될 수 있다. 본원에 기술된 것과 같이 DNA 피코르나바이러스 백신에 대한 투여량은, 전체적으로, 치료 대상, 항체들을 합성하기 위한 상기 숙주 세포 면역계의 능력, 및 요구된 보호의 정도에 의존하여, 투여(dose) 당 예비-조립된 피코르나바이러스 VLPs 및/또는 투여 당 보조제 분자의 1 내지 1000 ㎍, 전형적으로 5 내지 500 ㎍, 더 전형적으로 50 내지 550 ㎍, 더 전형적으로 100 내지 200 ㎍이다. 투여될 필요가 있는 활성 성분의 정확한 양은 수의사 또는 의사의 판단에 의존할 수 있고, 또는 각각의 대상에 특이적 일 수 있으나, 그러한 결정은 이러한 실시자의 기술 내에 있다.
본원의 백신과 같은 면역원성 조성물은, 단일 투여(single dose)로 제공될 수 있다; 2 회 투여 계획, 예를 들어, 2 내지 8주의 간격; 또는 다중 투여 계획으로 제공될 수 있다. 다중 투여 계획은, 1차 백신 접종 과정이 1 회 내지 10 회 또는 그 이상의 개별 투여를 포함할 수 있는 투여에 이어서 상기 면역 반응을 유지 및/또는 강화하는데 필요한 후속 시간 간격으로 투여되는 추가 투여 (예를 들어, 2차 투여의 경우 1 내지 4 개월, 10 개월 간격으로, 그리고 필요한 경우 수 개월 후의 후속 투여).
본원의 추가 측면들은, 본원의 조성물, 예를 들어, 본원에 기재된 백신 조성물와 같은, 면역원성 조성물에 혼입될 수 있는, 피코르나바이러스 바이러스-유사 입자 (VLP), FMDV 바이러스-유사 입자를 제조하는 방법에 관한 것이다. 본원의 특정 구현예에서, FMDV VLP와 같은 피코르나바이러스 VLP는 피코르나바이러스 P1 폴리펩타이드 전구체를 본원의 단리된 폴리뉴클레오타이드에 의해 코딩된 변형된 피코르나바이러스 3C 프로테아제 또는 3CD 융합체(fusion)와 접촉시킨 후에 생성된다.
일부 구현예들에 있어서, 본원의 피코르나바이러스 P1 폴리펩타이드 전구체 및 변형된 피코르나바이러스 3C 프로테아제, 또는 3CD 융합체는, 본원에 기술된 변형된 피코르나바이러스를 코딩하는 적어도 변이체 뉴클레오타이드 서열 3C 프로테아제, 또는 3CD 융합체 및 피코르나바이러스 P1 폴리펩타이드 전구체를 포함하는 형질전환 유전자 발현 카세트를 담지하는 재조합 벡터로 형질전환된 숙주 세포에서 in vivo 공동-발현된다. 상기 발현된 피코르나바이러스 P1 폴리펩타이드 전구체는 본원의 상기 발현된 변형된 피코르나바이러스 3C 프로테아제 또는 3CD 융합체에 의해 전체적으로 가공되어 VP1, VP2, VP3 및 VP4 또는 VP0, VP1 또는 VP3의 개별 피코르나바이러스 캡시드 단백질로 절단될 수 있고, 상기 캡시드 단백질은 자기조립되어 빈 피코르나바이러스 캡시드를 형성한다. 피코르나바이러스 VLP는 조립되어 하나 이상의 피코르나바이러스 캡시드를 형성할 수 있고, 이것은 그것들의 숙주 세포로부터 단리 및 정제될 수 있다.
다른 구현예들에 있어서, 피코르나바이러스 VLP 생성은 in vitro에서 일어난다. 이러한 구현예들에 있어서, VLP는, 예를 들어, 적절한 용액, 완충 및/또는 배양 배지를 이용하는 시험관에서 또는 페트리 접시에서 생성된다. 이후, in vitro 생성된 상기 피코르나바이러스 VLPs는 in vitro 환경으로부터 단리 및 정제될 수 있다.
[실시예]
실시예 1. 재료 및 방법
1A. pJJP 돌연변이 구조체의 단리
형질전환 유전자 발현에 대한 변형된 FMDV 3C 프로테아제의 효과를 평가하기 위해, 처음에, 폴리뉴클레오타이드 구조체를 FMDV O1 Manisa iso87 (Accession : AY593823)로부터의 P1-2A 폴리펩타이드를 코딩하는 서열, FMDV Asia1 Shamir 3C (Accession : AY593800) 및 Δ1D2A-SGLuc (Δ1M) 분비된 바이오마커를 포함하는 pJJP 플라스미드(GenScript Inc., Piscataway, NJ)에서 합성하였다. 형질전환 유전자 발현을 위한 리포터로서의 2A 번역 인터럽터 키메라에 대한 설명은 Puckette 등, BMC Biotechnol., 2017, 17:52를 참조하고, 상기 문헌의 전문은 본원에 참조로서 포함된다. 선택 제한 절단 부위는 복제를 용이하게 하기 위해 사일런트(silent) 돌연변이를 사용하여 제거되었다. 상기 폴리뉴클레오타이드 구조체들을 플라스미드 전파에 대한 제조사의 지시에 따라 New England Biolabs (NEB) 5-Alpha competent E. coli (New England Biolabs Inc., Ipswitch, MA)에 형질전환되었다. 배양액은 밀리미터 (μg/mL) 카베니실린 당 100 마이크로그램을 사용하여 테리픽 브로스 (terrific broth)에서 분당 250 회전 (rpm)으로 37℃에서 밤새 성장시켰다. 플라스미드는 QIAGEN® Plasmid Maxi 키트 (Qiagen Inc., Valencia CA)를 사용하여 단리되었다.
1B. 3C 프로테아제의 무작위 돌연변이유발
FMDV 3C 프로테아제의 무작위 돌연변이유발을 Takara Holdings Inc., Shimogyo-ku, Kyoto, Japan를 사용하여 제조사의 추천에 따라 1,000bp 당 2 개의 돌연변이체를 생성하였다. 증폭을 위해 사용된 프라이머들은 Nde-3C-F2 (CATATGAGTGGTGCCCCACCGAC, 서열 번호 641) 및 Rand-XhoXba-R (CCGATTCTAGACTCGAGTTA, 서열 번호 642)이고, 사용된 주형은 pUC57kan (enScript Inc.)에서 합성된 야생형 FMDV Asia1 Shamir 3C (Accession : AY593800)를 이용하였다. QIAQUICK® PCR 정제 키트 (Qiagen Inc.)를 사용하여 PCR 정제가 수행되었고, 제한 효소 NdeI 및 XhoI (New England Biolabs Inc.)에 의해 제조사가 제안한 것과 같이, PCR 산물을 분해하였다. 분해된 PCR 산물을, 제조사의 제안에 따라, T7 리가아제 (Roche Holding AG Basel, 스위스)를 사용하여, 유사하게 가공된 pSNAP-태그 (T7)-2 벡터 (New England Biolabs Inc.)에 복제시켰다.
1C. 박테리아 플레이팅(plating) 및 발현
T7 Express Competent E. coli의 형질전환 (New England Biolabs Inc)은 제조사에 의해 제안된 것과 같이 수행되었다. 플레이팅하기 전에, 형질전환된 대장균은 100 μg/mL 카베니실린 테리픽 브로스 10 mL에서 37℃에서 3 시간 동안 배양되었다. 배양 후, 200 μL를 이소프로필 β-D-1-티오갈락토피라노시드 (IPTG), 카베니실린 (Carb), 루리나-베르타니[Luria-Bertani (LB)] 플레이트 (IPTG +CARB LB 플레이)에 스프레딩하고, 37℃에서 밤새 배양하였다. 개별 대장균 콜로니들을 QIAPREP® Spin Miniprep 키트 (Qiagen Inc.)를 사용하여 플라스미드 단리를 위해 밤새 4 mL의 +CARB LB 배지에서 성장시켰다. 뉴클레오타이드 서열의 결정을 위해 프라이머 T7 (TAATACGACTCACTATAGGG, 서열 번호 643) 및 pSNAP-SR (CGGATATAGTTCCTCCTTTC, 서열 번호 644)을 사용하여 단리된 플라스미드를 시퀀싱하였다. 이어서, 목적 돌연변이를 코딩하는 플라스미드를 T7 Express Competent E. coli에 다시 형질전환 시켰고, 브로스에서 배양되었고, 상기 기재된 것과 같이, 한천(agar)에서 성장시켰다. 그 전문이 본원에서 참조로서 포함되는, Puckette 등, J. Virol., 2017, 91: e00924-17 (페이지 1-13) 참조.
1D. 형질전환 유전자 발현 및 가공 평가
293 성장 배지 [1X Dulbecco's Modified Eagle's medium (DMEM)], 10% 태아 소 혈청, 1X 항생제-향균제 및 1 X 비필수 아미노산을 사용하여 6-웰(well) 플레이트 (VWR International LLC, Radnor, PA)에서 HEK293T 세포 (ATCC CRL-3216)를 성장시켰다. 형질주입을 위해, 세포들을 2 mL Dulbecco 인산염-완충 생리 식염수 (DPBS)로 세척하였다. 4 μg의 플라스미드 DNA 및 10 μL의 리포펙타민 2000 (Invitrogen Inc., Carlsbad, CA)으로 형질주입하기 전에 각 웰에 1 mL의 새로운 배지를 첨가하였다. 수확하기 전에 세포들을 37℃에서 밤새 CO2 인큐베이터에서 성장시켰다.
루시퍼라아제 활성은, 각각의 웰에서 1:4 형질도입 유전자 배지-희석 샘플 100 μL을 사용하여 96-웰 BioSystems VERITAS™ 조도계 (Turner Biosystems Co., Sunnyvale, CA)를 사용하는 형질주입된 세포들로부터 수확된 배지에서 검출되었다. 판독 값은 50 μg/μL 수용성 코엘렌테라진 용액 (NANOLIGHT™ Technologies, Prolume, Ltd Pinetop AZ) 100 μg/μL를 주입한 후 바로, 기질의 주입 전과 후에 0.5 초의 집적 시간을 사용하여 수득하였다.
97℃에서 10 분간 가열된, 리튬도데실설페이트 (LDS) 샘플 완충액 (Invitrogen Inc., Carlsbad, CA)을 10-웰 NUPAGE® 4-12% Bis-Tris 젤 (Invitrogen Inc.)로 로딩하였다. 젤들은 200 V에서 35 분 동안 1X 2-(N-모르폴리노)에탄술폰산 (MES)에서 전기영동되었고, 그 후 상기 IBLOT® 2 시스템 (Invitrogen Inc.)을 사용하여 멤브레인 상에 전달되었다.
멤브레인을 실온에서 1 시간 동안 5% 밀크 블로킹(milk blocking) 완충액에서 배양한 다음, 1X PBS-TWEEN® Tablets (MilliporeSigma Inc., St. Louis, MO)으로 각각 5 분 동안 3 회 세척하였다. 1차 항체는 1:50 희석 쥐 모노클로날 항체 F1412SA (항-VP2) 및 12FE9.2.1 (항-VP1), 항-VP3 토끼 폴리클로날 항체에 대해 1 : 250 희석, 및 eIF4AI(ab31217, ABCAM)에 대해 1 : 1000 희석액에 첨가하고 실온에서 1 시간 동안 배양하였다. 그 전문이 본원에 참조로서 포함된, 항-VP1에 대한 Yang 등 Veterinary Immunology and Immunopathology, 2007, 115: 126-134 (2007) 및 항 VP2에 대한 Stave 등, The Journal of General Virology, 1986, 67: 2083-2092 참조.
멤브레인을 상기 1차 항체 배양 후 5 분 동안 TWEEN® 20 (PBST)의 1X 인산염 완충 생리 식염수로 상기 멤브레인들을 3 회 세척하였다. 2차 항체인 염소 항-쥐-양 고추냉이 퍼옥시다아제 (HRP) 및 염소 항-토끼-HRP (KPL)의 1 : 500 희석액을 상응하는 멤브레인에 실온에서 1 시간 동안 적용하였다. 1x PBST로 3 회 최종 세척 후, 제조사에 의해 제안된 것과 같이, SIGMAFAST? 3,3'-디아미노벤지딘 정제 (MilliporeSigma Inc)를 사용하여 시각화를 수행하였다.
실시예 2. 결과
2A. B 2 β 가닥 잔기(FMDV 잔기 123-128)의 돌연변이
FMDV 3C 프로테아제 결정 구조에서, L127 잔기는 A2 β 가닥과 β 시트를 형성하는 B2 β 가닥 상에 위치된다 (도 13a). 또한, FMDV 프로테아제 3C 결정 구조에 관하여 그 전문이 본원에 참조로서 포함된 Birtley 등, 2005, The Journal of Biological Chemistry 280: 11520-11527 (2005), Zunszain 등, Journal of Molecular Biology, 2010, 395: 375-389 and Yang 등, 2016, PeerJ 4: 4e (2016) 참조. 프롤린은 가닥 및 β 시트 구조를 파괴하는 것으로 알려져 있으므로, 본 발명자들은 FMDV O1 Manisa (O1M) P1, 3C 프로테아제 및 Δ1D2A-GLuc Δ1M 바이오 마커를 코딩하는 발현 플라스미드를 사용하여 123 내지 128의 각 잔기에 대해 프롤린 돌연변이유발 스캐닝을 수행하였다 (도 13b). L127P를 포함하지 않는 상기 다섯 개 시험된 잔기들 중 네 개가 야생형에 대해 형질전환 유전자 발현을 향상시켰다 (도 13c). 상기 네 가지 돌연변이들 중, V124P는 야생형에 비해 가장 강한 향상을 보였으나, D123P, G125P, 및 I128P는 V124P 활성의 1/3 미만을 나타내었다. 상기 L127P 돌연변이는 B2 β 가닥 프롤린 돌연변이체에서 형질전환 유전자 발현을 가장 많이 향상시켰다. 흥미롭게도, 돌연변이체 R126P는 야생형과 형질전환 유전자 발현에 차이가 없었다 (도 13c).
각각의 돌연변이에 대해, 형질전환된 HEK293T 세포로부터의 세포 용해물(lysates)을 상기 FMDV O1M P1 폴리펩타이드의 가공에 대해 조사하였다 (도 13d). 촉매성 트리아드에서의 C163A 활성 녹아웃(knockout) 돌연변이와는 달리, 상기 돌연변이 여섯개 모두는 상기 O1M P1 폴리펩타이드를 가공하는 능력을 유지하고 전체적으로 가공된 VP2를 나타내었다 (도 13d). 3C 프로테아제는 VP0을 VP4와 VP2로 분리하지 않는다. 그러한 분리는 VLPs의 형성 동안 VP0, VP3, 및 VP1 사이의 적절한 상호 작용에 의존하는 것으로 보고되었다.
B. 무작위 돌연변이유발 잔기들
L127P의 것과 유사한 표현형 (phenotype)을 수득하는 다른 신규 돌연변이를 확인하기 위해, 본 발명자들은 무작위 돌연변이유발 반응을 수행하였고 대장균 박테리아에서 발현을 유도하여 생존가능성 및 콜로니 성장을 향상시키는 3C 프로테아제 돌연변이들을 우선적으로 선택하였다. 다섯개의 다른 돌연변이가 확인되었다. 두개의 돌연변이들은 조기 성숙한 정지 코돈을 보유하였고 더 평가되지 않았다. 대장균의 복제물(clone)에 개별적으로 존재하는 나머지 돌연변이, T100P, L23P 및 L43H를 +CARB/+IPTG 및 +CARB 한천 성장 배지 모두에서 성장하여 대장균의 성장에 대한 영향을 평가하였다. 도 14에 나타낸 것과 같이, L23P 및 T100P만이 대장균 콜로니 성장을 향상시켰다.
상기 HEK293T 포유동물 세포에 대한 평가를 위해, 본 발명자들은, 상기 향상이 잔기 특이적인지, 또는 대신에 프롤린 삽입으로 인한 구조 파괴의 결과 인지를 더 잘 이해하기 위해, 인접 돌연변이체 I22P 및 I99P를 포함시켰다. HEK293T 세포들은 WT, L127P 및 C163A와 비교하여 돌연변이 I22P, I23P, I99P 또는 T100P를 포함하는 pJJP 플라스미드 (도 13b)로 형질주입시켰다. 네 가지의 신규 돌연변이 모두는 야생형과 비교했을 때, 형질전환 유전자 발현이 증가한 것으로 나타났다. 도 15a에 나타낸 것과 같이, 상기 형질전환 유전자 발현 수준은 상기 I22P 돌연변이 및 L127P 사이에서 비교 가능하다.
각각의 돌연변이에 대해, 형질주입된 HEK293T 세포로부터의 세포 용해물을 상기 FMDV O1M P1 폴리펩타이드의 가공에 대해 조사하였다(도 13d). 모든 돌연변이들은, C163A 활성 녹아웃 (knockout)을 제외하고, 상기 O1M P1 폴리펩타이드를 가공하는 능력을 유지하고 전체적으로 가공된 VP2를 나타내었다 (도 13d). VP0의 VP4 및 VP2로의 분리는 3C 프로테아제에 의해 가공되지 않고, VLPs의 형성 동안 VP0, VP3 및 VP1 사이의 적절한 상호작용에 의존하는 것으로 기재되었다.
세포 용해물을 웨스턴 블롯팅(western blotting)에 의한 P1 가공에 대해 평가하였다. 상이한 가공 정도가 측정되었지만, 새로운 돌연변이체 중 어느 것도 P1뿐만 아니라 L127P 돌연변이체를 가공하지 않았다 (도 15b). 소량이 검출될 수 있음에도 불구하고, 돌연변이체 I99P에 의한 단백질 가공의 표시는 나타나지 않았다 (도 15b). 돌연변이체 T100P는, VP2, 가공되지 않은 P1, 및 VP3 및 VP1의 융합 펩타이드(VP3/VP1)의 존재에 의해 나타난 것과 같이, VP1을 부분적으로 가공하였다. I22P와 L23P 모두 유사한 가공 프로파일을 보여주었고, P1은 거의 감지되지 않았다. 그러나, VP3/VP1의 존재는 VP3과 VP1의 접합(junction)을 가공하는 데 어려움을 시사하였다 (도 15b).
돌연변이 I22P, L23P, 및 T100P는 상기 O1M P1 폴리펩타이드를 가공하는 능력을 유지하고 전체적으로 가공된 VP2를 나타내었다 (도 15b). 상기 나타낸 것과 같이, VP0의 VP4 및 VP2로 분리는 3C 프로테아제에 의해 가공되지 않았고, VLPs의 형성 동안 VP0, VP3 및 VP1 사이의 적절한 상호작용에 의존하는 것으로 보고되었다.
2C. 확인된 돌연변이의 조합
돌연변이들은 3Cpro 내의 상이한 구조체로의 변경을 나타낸다. 돌연변이 V124P 및 L127P는 상기 B2 β 가닥 상에 존재하는 반면, I22P는 A1 β 가닥 상에 존재하고 T100은 F1 및 A' β 가닥들 사이의 루프 상에서 발견된다 (도 16). 3Cpro 활성에 대한 그것들의 효과를 결정하기 위해, 본 발명자들은 본원에서 확인된 3 가지 돌연변이체들, 즉 I22P, T100P 및 V124P와 L127P의 조합물을 제조하였다. 도 17a에 나타낸 것과 같이, I22P/T100P/V124P의 상기 형질전환 유전자 발현 수준은 I2P/T100P/L127P [~7.0Х1010 상대 루시퍼라아제 단위 (RLU)/0.5 초 vs ~4.5Х1010 RLU/0.5 초]의 것을 초과하였다.
I22P/L127P, T100P/L127P, 및 V124P/L127P의 세 개의 이중 돌연변이 구조체 및 I22P/T100P/V124P 및 I22P/T100P/L127P의 두 개의 트리플 돌연변이체는 pJJP 플라스미드를 사용하여 발현되었고 P1 가공을 평가하였다 (도 17b). 상기 V124P/L127P 이중 돌연변이체 구조체만 높은 수준의 가공을 유지하였다 (도 17b). I22P/L127P 구조체는 약한 가공을 유지하였지만, 전체적으로 가공된 생산물을 웨스턴 블롯(Western blots)에 의해 나타내지 않았다. 가공된 P1은 T100P/L127P 또는 트리플 돌연변이체를 포함하는 구조체에 대한 웨스턴 블롯에 의해 검출되지 않았다.
2D. FMDV 3C 프로테아제 돌연변이체의 eIF4AI에 대한 효과
3Cpro 는 진핵생물 세포의 번역 기계, eIF4AI의 성분을 가공한다. 형질주입된 HEK293-T 세포 용해물을, WT 및 C163A와 비교하기 위하여, 단일 돌연변이체 I22P, L23P, T100P, V124P, G125P 및 L127P뿐만 아니라, 이중 돌연변이체 I22P/L127P 및 V124P/L127P에 의해 가공된 숙주 세포 eIF4AI에 대해 조사하였다 (도 18). 가공된 eIF4AI에 상응하는 강한 밴드가 WT 및 R126P에서 관찰되었다; 약한 밴드가 G125P에서 관찰되었고, 매우 희미한 밴드가 V124P에서 검출되었다 (도 18).
2E. CHO-K1 및 BHK-21 세포주의 돌연변이 시험
중국 햄스터 난소 (CHO) 세포주는 생물의약 제조를 위해 가장 널리 사용되는 세포주 중 하나이다. 3Cpro에 대한 확인된 돌연변이가 야생형에 비해 형질전환 유전자 발현을 계속 향상시키는지 및 VLP 배열 형성이 유지되는 지 확인하기 위해 널리 사용되는 것을 선택하였다. 돌연변이체 L127P는 단일 돌연변이 중 가장 높은 루시퍼라아제 수준을 나타냈지만, HEK293-T 세포들의 생산량을 증가시키는 모든 돌연변이들 또한 CHO-K1 세포에서의 생산량을 향상시켰다 (도 23).
아기 햄스터 신장 (BHK-21) 세포주는 FMDV 불활성화 백신의 생산에 오랫동안 사용되어 왔다. 형질주입된 BHK-21 세포 돌연변이 I22P의 사용은 가장 높은 루시퍼라아제 수준을 나타내었고, 표준 편차 범위 내에서 약간 낮은 V124P를 나타내었다 (도 23). 돌연변이체 L127P는, 여전히 WT보다 월등히 우수하지만, I22P, T100P, 및 V124P에 비해 감소된 향상을 나타내었다 (도 23).
시험된 3Cpro 돌연변이체의 CHO-K1 및 BHK-21 세포주에서의 가공 효율은 HEK293-T 세포에서 관찰된 것과 유사하였다 (도 24a). 전자 현미경 검사는 형질주입된 CHO-K1 세포 (도 24b)에서 L127P 돌연변이체 및 형질도입된 BHK-21 세포에서 V124P 돌연변이체 (도 24c)로 VLP 형성을 확인하였다.
2F. 다른 피코르나바이러스의 동족성 잔기들
본 발명자들은 FMDV 3C 프로테아제 [Zunszain 등 Journal of Molecular Biology, 2010, 395:375-389) 4 (2016), 그 전문이 본원에 참조로서 포함됨]의 공개된 결정 구조와 연관하여 다섯 개의 추가 피코르나바이러스의 3C 프로테아제 결정 구조를 조사하였다. 상기 다섯 개의 추가 피코르나바이러스 3C 프로테아제 결정 구조는 폴리오바이러스 (Mosimann 등, J. Mol. Biol., 1997, 273: 1032-1047), 인간 리노바이러스 (Kawatkar 등, Bioorg. Med. Chem. Lett., 2016, 26: 3248-3252), 인간 엔테로바이러스 71 (Wang 등, Journal of Virology, 2011, 85: 10021-10030), 콕사키바이러스 B3 (Lee 등, J. Biol. Chem., 2009, 284: 7646-7655) 및 A 형 간염 바이러스 (Bergmann 등, Journal of Virology, 1997, 71: 2436-2448)이고, 그 전문이 본원에 참조로서 포함된다.
2G (i) 다른 피코르나바이러스들의 V124P 및 L127P에 대한 동족성 잔기들
공개된 FMDV 3Cpro 결정 구조에서, A2-B2 β 시트는 기질 결합 포켓의 성분은 아니지만, C163 잔기를 포함하는 루프에 매우 근접되어 있다 [Birtley 등, 2005, The Journal of Biological Chemistry, 280: 11520-11527, Zunszain 등, Journal of Molecular Biology, 2010, 395: 375-389, Yang 등, PeerJ, 2016, 4: 4e (1964), 그 전문이 본원에 참조로서 포함됨]. 이러한 발견은 상기 A2-B2 β 시트가 기질 특이성에서 역할을 한다는 추론을 이끌어 냈다 [Birtley 등, 2005, The Journal of Biological Chemistry, 280: 11520-11527, 그 전문이 본원에 참조로서 포함됨]. 잔기 V124 및 L127의 사이드 체인은, 촉매성 잔기 C163에 인접한 잔기 Y162의 사이드 체인에 대하여 상기 B2 β 가닥 상에 가장 가까운 잔기이다 (도 19a 및 도 19b). 상기 A2 가닥의 잔기 I119 및 N121과 함께, 이들 잔기의 사이드 체인들은 Y162의 사이드 체인을 향해 있다(도 19a). 이것은 상기 B2 β 가닥, 3Cpro 결합 포켓, 및 촉매성 트리아드에 대한 접촉점을 제공한다. 돌연변이 V124P 또는 L127P 어느 것도 단백질 분해 활성을 감소시키지 않았고, 이것은 상기 촉매 트리아드에 심각한 영향을 미치지 않았음을 시사한다. 잔기 Y162에 대한 V124P 및 L127P의 가까운 근접부(proximity)는 프롤린에 대한 각각 잔기의 돌연변이가 3Cpro의 숙주 세포 단백질을 쉽게 가공하는 능력을 감소시키는 수단을 제공할 수 있다 (도 19b). 유사한 구조가 가닥 구조의 상기 사이드 체인과 연관되어 촉매성 시스테인에 바로 인접한 잔기의 위치를 조사함으로써 다른 피코르나바이러스에서 관찰된다 (도 20). 촉매성 시스테인은 FMDV에서 잔기 163, A 형 간염 바이러스에서 172, 폴리오바이러스에서 147, 인간 리노바이러스, 인간 엔테로바이러스 71 및 콕사키바이러스 B3이다. 이것은 A 형 간염 바이러스에 대한 잔기 171과, 폴리오바이러스, 인간 리노바이러스, 인간 엔테로바이러스 71, 및 콕사키바이러스 B3에 대한 잔기 146에 대한 가까운 가닥 구조체의 사이드 체인의 상호작용을 조사하는 것을 가능하게 한다. 본 발명자들은 다음과 같은 잔기가 돌연변이된 경우 3C 프로테아제의 숙주 세포 단백질에 대한 악영향을 감소시킬 수 있음을 제안한다: A 형 간염 바이러스에 대한 M128, I130, T121 및 L119; 폴리오바이러스의 경우 V104, L102 및 T142; 인간 리노바이러스의 경우 Q108, T112, I114, L102 및 L104; 인간 엔테로바이러스 71의 경우 T106, M109 및 I104; 콕사키바이러스 B3의 경우 M112, I114, F109, I104 및 L102.
2G (ii). 다른 피코르나바이러스의 I22P 및 L23P에 동족성 잔기들
FMDV에서, 잔기 I22P 및 L23P는 상기 B1 β 가닥과 쌍을 이루는 상기 A1 β 가닥의 일부이다 (도 19c). 잔기 18-31을 포함하는 상기 A1-B1 β 가닥 쌍은 상기 펩타이드 결합 포켓에 인접하고 (도 18c), 기질에 결합 시 구조적 변화를 겪는다. 그 전문이 본원에 참조로서 포함된, Zunszain 등, Journal of Molecular Biology, 2010, 395: 375-389 참조. 이것은 상기 돌연변이 I22P 및 L23P가 A1-B1 β 가닥 쌍의 구조를 변화시키는 상기 A1 β 가닥 구조의 변경을 통해 기질 결합에 영향을 줄 수 있음을 제시한다. 이러한 변경은, 기질 특이성을 파괴하는 결합 포켓의 크기 및 모양에 영향을 미칠 수 있다.
유사한 구조들이 N-말단 나선(helical) 도메인에 연결된 β-시트 구조의 잔기를 조사함으로써 다른 피코르나바이러스에서 관찰되었다 (도 21). 이것은, 돌연변이된 경우, 3C 프로테아제 숙주 세포 단백질 가공이 감소될 수 있는 하기 잔기의 확인을 가능하게 한다: 폴리오바이러스에서 잔기 T20 및 T19, 인간 리노바이러스에서 T20 및 T19, 인간 엔테로바이러스 71에서 T20 및 Q19, 콕사키바이러스 B3에서 T20 및 K19, 및 A 형 간염 바이러스에서 E20 및 G19 (도 21).
2G (iii) 다른 피코르나바이러스의 T100P와 동족성인 잔기들
FMDV에서, 돌연변이 I99P 및 T100P는 인접하고 있음에도 불구하고 상이한 P1 가공 결과를 나타낸다. 둘 다는 상기 결합 부위로부터 원위(distal)이고, A2' β 가닥과 상기 F1 β 가닥 사이의 루프 내에 위치되고, 상기 N-말단 및 C-말단 α 나선에 가까운 근접부에 위치된다 (도 19d). T100P의 것과 대조적으로, I99P로 관찰된 총 가공에서의 파괴(disruptions)는 상기 말단 나선중의 하나 또는 둘 모두의 영향 및 상기 상호 작용으로 인한 단백질 구조의 후속 파괴를 통해 촉진될 수 있다.
유사한 구조가 다른 피코르나바이러스 3C 프로테아제 구조에서 관찰되었다. 특히, 상기 단백질의 상기 N-말단과 C-말단 사이에 위치된 β 가닥 구조를 따르는 루프 영역의 구조가 모든 구조들에 존재한다 (도 22). 이것은 돌연변이된 경우, 3C 프로테아제의 부작용을 감소시킬 수 있는 하기 잔기들의 확인을 가능하게 하였다: 폴리오바이러스에서 잔기 R87, 인간 리노바이러스에서 R87 및 R88, 인간 엔테로바이러스 71에서 T87 및 K88, 콕사키바이러스 B3에서 R87 및 L90, A 형 간염 바이러스에서 T100 및 Q101 (도 22).
실시예 3. 1차 아미노산 서열의 정렬
FMDV 3C 프로테아제의 1차 아미노산 서열과 다른 피코르나바이러스 종들로부터의 3C 프로테아제의 정렬은 도 4 내지 도 12에 나타내었다. 정렬은 Blossum 62, 기본 매개변수를 사용하여 Clone Manager 9, Professional Edition, 버전 9.4, 2015.01.01 을 사용하여 준비되었다. 1차 서열 배열은 FMDV 3C 프로테아제에서, 예를 들어, V124, L127, I22, L23 및 T100에 상응하는 비-FMDV 피코르나바이러스 3C 프로테아제의 상기 아미노산 잔기를 나타낸다. 상기 1차 서열 정렬에서 동정된 상응하는 아미노산은 전체적으로 상기 기술된 구조 동족성 분석을 사용하여 동정된 것들과 일치한다.
실시예 4. 폴리오바이러스 3C 및 3CD 프로테아제 돌연변이체의 형질전환 유전자 발현을 향상
3D 폴리머라아제 (Gluc-2A-3CD)와 융합된 가우시아 루시퍼라아제 (Gaussia luciferase), 2A 번역 인터럽터 및 인간 폴리오바이러스 1 균주 Mahoney (PV) 3C 프로테아제 돌연변이체 (Gluc-2A-3C) 또는 PV 3C 프로테아제 돌연변이체를 포함하는 구조체가 제조되었다. 이러한 구조체들은 도 25에 나타내었다.
기술된 Gluc-2A-3C 및 Gluc-2A-3CD 구조체들은 하기 PV 돌연변이체들에 대해 형질전환 유전자 발현을 평가하기 위해 사용되었다: PV 3C (R87P), PV 3CD (R87P), PV 3C (L102P) 및 PV 3CD (L102P). 형질전환 유전자 발현을 평가하기 위해, 형질주입된 HEK293-T 세포로부터의 세포 배양 배지 상의 항-gluc 항체를 사용하는 웨스턴 블롯을 사용하였다. FMDV 3C (wt), PV 3C (wt), 활성 녹아웃(knockout) 돌연변이체 FMDV 3C (C163A), 활성 녹아웃(knockout) 돌연변이체 PV 3C (C172A) 및 활성 녹아웃(knockout) 돌연변이체 PV 3CD (C172A)를 포함하는 발현 카세트를 대조군으로 사용하였다. 도 26에서 명백히 나타낸 것과 같이, 상기 3C 구조체에 대한 야생형 (서열 번호 579)에 비해, 돌연변이체 R87P (서열 번호 571) 및 돌연변이체 L102P (서열 번호 569)에서 향상된 형질전환 유전자 발현이 관찰되었다. 상기 향상은 야생형 발현이 쉽게 검출되지 않는 3CD 구조체들에 대해 더욱 명확하였다.
SEQUENCE LISTING <110> U.S. DEPARTMENT OF HOMELAND SECURITY PUCKETTE, Michael NEILAN, John <120> MODIFIED PICORNAVIRUS 3C PROTEASES AND USES THEREOF <130> DHS-0162 <160> 655 <170> PatentIn version 3.5 <210> 1 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> WT_A_2006 <400> 1 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 2 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 2 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 3 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> WT_A24 <400> 3 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc atc ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 4 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 4 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 5 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> WT_Asia_leb_89 <400> 5 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 6 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 6 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 7 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> WT_Asia1_Shamir <400> 7 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ttg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 8 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 8 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 9 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <223> WT_C3 <220> <221> misc_feature <222> (1)..(639) <223> WT_C3 <400> 9 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg att ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 10 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 10 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 11 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> WT_O_pan_asia <400> 11 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ata ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 12 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 12 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 13 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> WT_O1_Manissa <400> 13 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 14 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 14 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 15 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> WT_SAT1 <400> 15 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc atc ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 16 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 16 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 17 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> WT_SAT2_Egypt_2010 <400> 17 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ata ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac gtt ggg aga ctc atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 18 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 18 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 19 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> WT_SAT3 <400> 19 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 20 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 20 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 21 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> D123P_A_2006 <400> 21 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct ccn gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 22 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 22 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 23 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> D123P_A24 <400> 23 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc atc ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc ccn gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 24 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 24 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 25 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> D123P_Asia_leb_89 <400> 25 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc ccn gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 26 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 26 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 27 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> D123P_Asia1_Shamir <400> 27 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc ccn gtt ggg aga ttg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 28 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 28 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 29 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> D123P_C3 <400> 29 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg att ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc ccn gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 30 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 30 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 31 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> D123P_O_pan_asia <400> 31 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ata ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc ccn gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 32 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 32 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 33 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> D123P_O1_Manissa <400> 33 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc ccn gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 34 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 34 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 35 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> D123P_SAT1 <400> 35 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc atc ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc ccn gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 36 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 36 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 37 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> D123P_SAT2_Egypt_2010 <400> 37 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ata ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc ccn gtt ggg aga ctc atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 38 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 38 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 39 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> D123P_SAT3 <400> 39 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct ccn gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 40 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 40 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Pro Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 41 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> G125P_A_2006 <400> 41 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat gtc ccn aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 42 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 42 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 43 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> G125P_A24 <400> 43 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc atc ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat gtc ccn aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 44 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 44 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 45 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> G125P_Asia_leb_89 <400> 45 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ccn aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 46 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 46 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 47 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> G125P_Asia1_Shamir <400> 47 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ccn aga ttg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 48 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 49 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> G125P_C3 <400> 49 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg att ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac gtt ccn aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 50 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 50 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 51 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> G125P_O_pan_asia <400> 51 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ata ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat gtc ccn aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 52 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 52 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 53 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> G125P_O1_Manissa <400> 53 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac gtt ccn aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 54 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 54 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 55 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> G125P_SAT1 <400> 55 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc atc ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac gtc ccn aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 56 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 56 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 57 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> G125P_SAT2_Egypt_2010 <400> 57 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ata ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac gtt ccn aga ctc atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 58 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 58 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 59 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> G125P_SAT3 <400> 59 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat gtc ccn aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 60 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 60 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Pro Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 61 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I128P_A_2006 <400> 61 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat gtc ggg aga ctg ccn 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 62 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 62 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 63 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I128P_A24 <400> 63 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc atc ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat gtc ggg aga ctg ccn 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 64 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 64 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 65 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I128P_Asia_leb_89 <400> 65 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ctg ccn 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 66 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 66 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 67 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I128P_Asia1_Shamir <400> 67 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ttg ccn 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 68 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 68 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 69 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I128P_C3 <400> 69 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg att ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac gtt ggg aga ctg ccn 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 70 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 70 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 71 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I128P_O_pan_asia <400> 71 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ata ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat gtc ggg aga ctg ccn 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 72 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 72 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 73 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I128P_O1_Manissa <400> 73 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac gtt ggg aga ctg ccn 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 74 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 74 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 75 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I128P_SAT1 <400> 75 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc atc ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac gtc gga aga ctc ccn 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 76 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 76 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 77 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I128P_SAT2_Egypt_2010 <400> 77 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ata ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac gtt ggg aga ctc ccn 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 78 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 78 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 79 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I128P_SAT3 <400> 79 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat gtc gga aga ctc ccn 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 80 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 80 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Pro 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 81 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P_A_2006 <400> 81 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 82 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 82 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 83 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P_A24 <400> 83 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ccn ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 84 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 84 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 85 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P_Asia_leb_89 <400> 85 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 86 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 86 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 87 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P_Asia1_Shami <400> 87 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ttg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 88 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 88 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 89 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P_C3 <400> 89 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg ccn ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 90 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 90 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 91 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P_O_pan_asia <400> 91 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ccn ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 92 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 92 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 93 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P_O1_Manissa <400> 93 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 94 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 94 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 95 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <223> I22P_SAT1 <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P_SAT1 <400> 95 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc ccn ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 96 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 96 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 97 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <223> I22P_SAT2_Egypt_2010 <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P_SAT2_Egypt_2010 <400> 97 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ccn ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac gtt ggg aga ctc atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 98 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 98 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 99 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P_SAT3 <400> 99 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc ccn ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 100 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 100 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 101 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I99P_A_2006 <400> 101 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac ccn acg aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Pro Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 102 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 102 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Pro Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 103 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I99P_A24 <400> 103 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc atc ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac ccn acg aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Pro Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 104 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 104 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Pro Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 105 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I99P_Asia_leb_89 <400> 105 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac ccn acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Pro Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 106 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 106 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Pro Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 107 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> 199P_Asia_Shamir <400> 107 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac ccn acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Pro Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ttg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 108 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 108 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Pro Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 109 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I99P_C3 <400> 109 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg att ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac ccn acg aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Pro Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 110 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 110 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Pro Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 111 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> 199P_O_pan_asia <400> 111 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ata ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac ccn acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Pro Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 112 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 112 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Pro Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 113 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> 199P_01_Manissa <400> 113 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac ccn acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Pro Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 114 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 114 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Pro Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 115 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> 199P_SAT2_Egypt_2010 <400> 115 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ata ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac ccn acg aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Pro Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac gtt ggg aga ctc atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 116 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 116 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Pro Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 117 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L127P_A_2006 <400> 117 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat gtc ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 118 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 118 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 119 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L127P_A24 <400> 119 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc atc ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat gtc ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 120 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 120 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 121 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L127P_Asia_leb_89 <400> 121 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 122 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 122 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 123 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L127P_Asia1_Shamir <400> 123 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 124 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 124 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 125 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L127P_C3 <400> 125 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg att ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac gtt ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 126 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 126 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 127 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L127P_O_pan_asia <400> 127 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ata ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat gtc ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 128 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 128 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 129 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L127P_01_Manissa <400> 129 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac gtt ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 130 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 130 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 131 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L127P_SAT1 <400> 131 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc atc ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac gtc gga aga ccn atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 132 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 132 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 133 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L127P_SAT2_Egypt_2010 <400> 133 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ata ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac gtt ggg aga ccn atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 134 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 134 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 135 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L127P_SAT3 <400> 135 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat gtc gga aga ccn atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 136 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 136 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 137 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> L23P_A_2006 <400> 137 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ccn gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 138 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 138 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 139 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> L23P_A24 <400> 139 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc atc ccn gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 140 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 140 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 141 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L23P_Asia_leb_89 <400> 141 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ccn gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 142 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 142 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 143 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L23P_Asia_Shamir <400> 143 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ccn gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ttg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 144 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 144 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 145 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L23P_C3 <400> 145 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg att ccn gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 146 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 146 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 147 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L23P_O_pan_asia <400> 147 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ata ccn gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 148 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 148 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 149 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L23P_01_Manissa <400> 149 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ccn gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 150 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 150 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 151 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L23P_SAT1 <400> 151 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc atc ccn gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 152 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 152 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 153 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L23P_SAT2_Egypt_2010 <400> 153 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ata ccn gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac gtt ggg aga ctc atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 154 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 154 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 155 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> L23P_SAT3 <400> 155 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc atc ccn gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 156 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 156 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Pro Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 157 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> L42H_A_2006 <400> 157 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac cay gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 158 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 158 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 159 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L42H_A24 <400> 159 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc atc ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac cay gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 160 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 160 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 161 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L42H_Asia_leb_89 <400> 161 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac cay gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 162 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 162 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 163 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> L42H_Asia1_Shamir <400> 163 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac cay gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ttg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 164 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 164 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 165 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L42H_C3 <400> 165 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg att ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac cay gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 166 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 166 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 167 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L42H_O_pan_asia <400> 167 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ata ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac cay gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 168 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 168 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 169 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L42H_01_Manissa <400> 169 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac cay gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 170 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 170 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 171 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L42H_SAT1 <400> 171 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc atc ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat cay gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 172 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 172 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 173 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L42H_SAT_Egypt_2010 <400> 173 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ata ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat cay gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac gtt ggg aga ctc atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 174 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 174 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 175 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L42H_SAT3 <400> 175 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac cay gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 176 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 176 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr His Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 177 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L99P_SAT1 <400> 177 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc atc ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ccn acg gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Pro Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 178 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 178 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Pro Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 179 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> L99P_SAT3 <400> 179 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ccn acg gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Pro Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 180 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 180 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Pro Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 181 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> R126P_A_2006 <400> 181 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat gtc ggg ccn ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 182 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 182 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 183 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> R126P_A24 <400> 183 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc atc ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat gtc ggg ccn ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 184 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 184 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 185 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> R126P_Asia_leb_88 <400> 185 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg ccn ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 186 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 186 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 187 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> R126P_Asia_Shamir <400> 187 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg ccn ttg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 188 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 188 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 189 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> R126P_C3 <400> 189 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg att ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac gtt ggg ccn ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 190 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 190 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 191 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> R126_O_pan_asia <400> 191 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ata ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat gtc ggg ccn ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 192 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 192 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 193 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> R126P_01_Manissa <400> 193 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac gtt ggg ccn ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 194 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 194 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 195 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> R126P_SAT1 <400> 195 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc atc ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac gtc gga ccn ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 196 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 196 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 197 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> R126_SAT_Egypt_2010 <400> 197 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ata ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac gtt ggg ccn ctc atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 198 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 198 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 199 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> R126P_SAT3 <400> 199 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat gtc gga ccn ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 200 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 200 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Pro Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 201 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P_A_2006 <400> 201 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 202 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 202 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 203 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P_A24 <400> 203 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc atc ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 204 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 204 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 205 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> T100P_Asia_leb_89 <400> 205 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 206 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 206 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 207 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P_Asia1_Shamir <400> 207 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ttg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 208 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 208 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 209 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P_C3 <400> 209 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg att ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 210 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 210 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 211 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P_O_pan_asia <400> 211 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ata ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc ccn aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Pro Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 212 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 212 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 213 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P_O1_Manissa <400> 213 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 214 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 214 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 215 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P_SAT1 <400> 215 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc atc ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc ccn gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 216 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 216 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 217 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P_SAT_Egypt_2010 <400> 217 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ata ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Pro Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac gtt ggg aga ctc atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 218 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 218 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 219 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P_SAT3 <400> 219 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc ccn gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 220 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 220 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 221 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P_A_2006 <400> 221 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat ccn ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 222 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 222 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 223 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P_A24 <400> 223 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc atc ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat ccn ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 224 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 224 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 225 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P_Asia_leb_89 <400> 225 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac ccn ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 226 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 226 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 227 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P_Asia1_Shamir <400> 227 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac ccn ggg aga ttg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 228 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 228 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 229 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P_C3 <400> 229 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg att ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac ccn ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 230 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 230 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 231 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P_O-pan_asia <400> 231 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ata ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat ccc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 232 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 232 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 233 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P_01_Manissa <400> 233 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac ccn ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 234 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 234 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 235 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P_SAT1 <400> 235 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc atc ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac ccn gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 236 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 236 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 237 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P_SAT2_Egypt_2010 <400> 237 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ata ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac ccn ggg aga ctc atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 238 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 238 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 239 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P_SAT3 <400> 239 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat ccn gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 240 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 240 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 241 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/L127P_A_2006 <400> 241 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat gtc ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 242 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 242 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 243 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/L127P_A24 <400> 243 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ccn ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat gtc ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 244 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 244 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 245 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/L127P_asia_leb_89 <400> 245 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 246 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 246 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 247 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/L127P_Asia1_Shamir <400> 247 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 248 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 248 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 249 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/L127_C3 <400> 249 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg ccn ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac gtt ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 250 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 250 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 251 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/L127P_O_pan_asia <400> 251 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ccn ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat gtc ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 252 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 252 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 253 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> I22P/L127P_01_Manissa <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <400> 253 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac gtt ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 254 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 254 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 255 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/L127P_SAT1 <400> 255 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc ccn ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac gtc gga aga ccn atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 256 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 256 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 257 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/L127P_SAT2_Egypt_2010 <400> 257 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ccn ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac gtt ggg aga ccn atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 258 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 258 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 259 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/L127_SAT3 <400> 259 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc ccn ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat gtc gga aga ccn atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 260 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 260 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 261 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P/L127P_A_2006 <400> 261 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat gtc ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 262 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 262 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 263 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P/L127P_A24 <400> 263 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc atc ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat gtc ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 264 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 264 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 265 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P/L127P_Asia_leb_89 <400> 265 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 266 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 266 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 267 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P/L127P_Asia1_Shamir <400> 267 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac ccn ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 268 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 268 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 269 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P/L127P_C3 <400> 269 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg att ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac gtt ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 270 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 270 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 271 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P/L127P_O_pan_asia <400> 271 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ata ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat ccn ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 272 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 272 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 273 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P/L127P_01_Manissa <400> 273 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac ccn ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 274 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 274 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 275 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P/L127P_SAT1 <400> 275 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc atc ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc ccn gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac gtc gga aga ccn atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 276 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 276 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 277 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P/L127P_SAT2_Egypt_2010 <400> 277 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ata ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Pro Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac gtt ggg aga ccn atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 278 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 278 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 279 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T100P/L127P_SAT3 <400> 279 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc ccn gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat gtc gga aga ccn atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 280 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 280 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 281 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T34A/M88L_A_2006 <400> 281 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct gcn gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc ctn gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 282 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <220> <221> misc_feature <222> (88)..(88) <223> The 'Xaa' at location 88 stands for Leu. <400> 282 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 283 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> T34A/M88L_24 <400> 283 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc atc ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct gcn gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc ctn gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 284 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <220> <221> misc_feature <222> (88)..(88) <223> The 'Xaa' at location 88 stands for Leu. <400> 284 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 285 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T34A/M88L_Asia_leb_89 <400> 285 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc gcn gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc ctn gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 286 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <220> <221> misc_feature <222> (88)..(88) <223> The 'Xaa' at location 88 stands for Leu. <400> 286 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 287 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T34A/M88L_Asia1_Shamir <400> 287 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc gcn gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc ctn gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ttg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 288 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <220> <221> misc_feature <222> (88)..(88) <223> The 'Xaa' at location 88 stands for Leu. <400> 288 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 289 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T34A/M88L_C3 <400> 289 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg att ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc gcn gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc ctn gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 290 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <220> <221> misc_feature <222> (88)..(88) <223> The 'Xaa' at location 88 stands for Leu. <400> 290 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 291 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T34A/M88L_O_pan_asia <400> 291 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ata ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct gcn gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc ctn gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 292 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <220> <221> misc_feature <222> (88)..(88) <223> The 'Xaa' at location 88 stands for Leu. <400> 292 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 293 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> T34A/M88L_01_Manissa <400> 293 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct gcn gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc ctn gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 294 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <220> <221> misc_feature <222> (88)..(88) <223> The 'Xaa' at location 88 stands for Leu. <400> 294 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 295 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T34A/M88L_SAT1 <400> 295 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc atc ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct gcn gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc ctn gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 296 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <220> <221> misc_feature <222> (88)..(88) <223> The 'Xaa' at location 88 stands for Leu. <400> 296 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 297 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T34A/M88L_SAT2_Egypt_2010 <400> 297 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ata ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc gcn gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc ctn gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac gtt ggg aga ctc atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 298 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <220> <221> misc_feature <222> (88)..(88) <223> The 'Xaa' at location 88 stands for Leu. <400> 298 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 299 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> T34A/M88L_SAT3 <400> 299 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg gcn gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc ctn gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat gtc gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 300 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <220> <221> misc_feature <222> (88)..(88) <223> The 'Xaa' at location 88 stands for Leu. <400> 300 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Ala Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Xaa Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 301 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P/L127P_A_2006 <400> 301 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat ccn ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 302 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 302 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 303 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P/L127P_A24 <400> 303 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc atc ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat ccn ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 304 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 304 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 305 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P/L127P_Asia_leb_89 <400> 305 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac ccn ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 306 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 306 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 307 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P/L127P_Asia1_Shamir <400> 307 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac ccn ggg aga tcn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Xaa Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 308 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <220> <221> misc_feature <222> (127)..(127) <223> The 'Xaa' at location 127 stands for Ser. <400> 308 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Xaa Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 309 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P/L127P_C3 <400> 309 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg att ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac ccn ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 310 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 310 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 311 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P/L127P_O_pan_asia <400> 311 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ata ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc acg aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat gtc ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 312 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 312 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 313 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P/L127P_01_Manissa <400> 313 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac gtt ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 314 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 314 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 315 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P/L127P_SAT1 <400> 315 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc atc ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac ccn gga aga ccn atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 316 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 316 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 317 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P/L127P_SAT2_Egypt_2010 <400> 317 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ata ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc acg aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac ccn ggg aga ccn atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 318 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 318 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Thr Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 319 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> V124P/L127P_SAT3 <400> 319 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc atc ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc acg gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat ccn gga aga ccn atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 320 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 320 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Ile Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Thr Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Pro Gly Arg Pro Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 321 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/L127P_A_2006 <400> 321 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat gtc ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 322 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 322 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 323 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/L127P_A24 <400> 323 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ccn ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat gtc ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 324 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 324 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 325 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/L127P_Asia_leb_89 <400> 325 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 326 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 326 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 327 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/L127P_Asia1_Shamir <400> 327 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac gtt ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 328 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 328 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 329 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/L127P_C3 <400> 329 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg ccn ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac gtt ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 330 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 330 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 331 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/L127P_O_pan_asia <400> 331 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ccn ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc ccn aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Pro Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat gtc ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 332 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 332 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 333 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/L127P_)1_Manissa <400> 333 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac gtt ggg aga ccn att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 334 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 334 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 335 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/L127P_SAT1 <400> 335 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc ccn ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc ccn gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac gtc gga aga ccn atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 336 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 336 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 337 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/L127P_SAT2_Egypt_2010 <400> 337 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ccn ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Pro Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac gtt ggg aga ccn atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 338 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 338 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 339 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/L127P_SAT3 <400> 339 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc ccn ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc ccn gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat gtc gga aga ccn atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 340 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 340 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Val Gly Arg Pro Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 341 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/V124P_A_2006 <400> 341 agt ggt gcc ccc ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctc gac ggg aag acg gta gcc atc tgt tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gct tac ctt gtg cca cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tat gac aag atc atg ctg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa ggg cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gat aca gca aga atg aag aag ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtt atc aac aac gct gat ccn ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tat tgt gga gga gct gtt ctt gca aag gac gga gcc gag acc ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gca ggt ggt aat gga gtt gga tac tgt tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctg cta aag atg aag gca cac atc gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 342 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 342 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 343 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/V124P_A24 <400> 343 agt ggt gcc cca ccg acc gac ttg caa aag ttg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ccn ctt gac ggg aag acg gta gcc att tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtt ttc ggc act gct tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac aag atc atg ttg gac ggc aga gcc atg aca gat 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtg atc aac aac gcc gat ccn ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tct ggt gaa gcc ctt acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggg ctc ttt gcc tac aaa gcc gca acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggt tat tgc gga gga gcc gtc ctc gct aag gac ggg gct gac acg ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc acc cac tcc gct gga ggc aat ggc gtt gga tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc agg tcc atg ctt ctc aag atg aag gca cac gtt gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 344 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 344 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Leu Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Val Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 345 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/V124P_Asia_leb_89 <400> 345 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac ccn ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 346 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 346 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 347 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/V124P_Asia1_Shamir <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> CDS <222> (1)..(639) <400> 347 agt ggt gcc cca ccg acc gac ttg caa aag atg gtc atg agc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctt gac ggt aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggt act gcc tac ctc gtg cct cgt cac ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gaa aag tac gac agg atc atg ttg gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gcg ctc atg gtg ctc cac cgt ggc aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cac ttt cgt gat aca gca aga atg aag aaa ggt 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggc gtg atc aac aac gcc gac ccn ggg aga ttg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tcc ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc cta ttt gcc tac aga gcc gct acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcc aag gac gga gct gac aca ttt 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tcc gca gga ggc aat gga gtc ggg tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gta tct agg tcc atg ctc ttg aag atg aag gca cac att gac ccc 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 348 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 348 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ser Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Arg Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 349 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22/T100P/V124P_C3 <400> 349 agc ggt gcc cca ccg acc gac ttg caa aag atg gtc atg ggc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctg ccn ctc gat ggg aag acg gtg gcc atc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc acc gga gtg ttt ggc act gct tac ctc gtg ccg cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gca ctc atg gtg cta cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aag cac ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gta ggc gtg att aac aac gcc gac ccn ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctt acc tac aaa gac att gtg gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgt gga gga gcc gtc ctt gcc aag gat gga gct gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtc ggc act cac tct gct gga ggc aat gga gtt ggt tac tgc tca 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aga tcc atg ctt ctc aag atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gag cca cac cac gag 639 Glu Pro His His Glu 210 <210> 350 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 350 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 351 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P?124P_O_pan_asia <400> 351 agt ggt gct cca ccg act gac ttg caa aag atg gtc atg ggt aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag ccc gtt gag ctc ccn ctt gac ggg aag aca gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct act gga gtg ttt ggc acc gcc tac ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ttg gac ggc aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gcc gcg ctc atg gtg ctc cac cgt ggg aat cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 cgg gac atc ccn aaa cac ttc cgt gat gtg gca aga atg aag aaa gga 336 Arg Asp Ile Pro Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtc gtt ggt gtt atc aac aac gcc gat ccn ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tct ggt gag gcc ctc acc tac aag gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg cct ggc ctc ttt gcc tac aag gcc gcc acc aag gcc 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctc gca aag gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc aca cac tct gcg ggc ggc aac gga gtt gga tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctg ctc aag atg aag gca cac ata gat cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cat gaa 639 Glu Pro His His Glu 210 <210> 352 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 352 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Val Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 353 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/V124P_01_Manissaa <400> 353 agt ggt gcc cca ccg acc gac ttg cag aag atg gtc atg ggc aac act 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 aag cct gtt gag ctc ccn ctc gac ggg aag acg gta gcc atc tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gct acc gga gtg ttt ggc act gcc tac ctc gta cct cgt cac ctc ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gcg gag aag tac gac aag ata atg ttg gac ggt aga gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 agt gac tac aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gac gct gca ctc atg gtg ctt cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aaa cat ttt cgt gac aca gca aga atg aag aaa ggc 336 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 acc ccc gtt gtc ggt gtg atc aac aac gcc gac ccn ggg aga ctg att 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tct gga gag gcc ctt acc tac aaa gac att gta gtg tgc atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 gga gac acc atg ccg ggc ctg ttt gcc tac aga gcc gcc acc aag gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 ggt tac tgc ggg gga gcc gtt ctc gcc aag gac gga gcc gac aca ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 atc gtt ggc act cac tcc gca ggt ggt aac gga gtt gga tac tgc tcg 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtg tcc agg tcc atg ctc ctg aaa atg aag gca cac att gac cct 624 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gag 639 Glu Pro His His Glu 210 <210> 354 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 354 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Gly Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Met Thr Asp 50 55 60 Ser Asp Tyr Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Thr Ala Arg Met Lys Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Asp Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Lys Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 355 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/V124P_SAT1 <400> 355 agc gga tgt cca ccg acc gac ctg cag agg atg gtc atg gca aac gtg 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 aaa ccc gtt gag ctc ccn ctc gat ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gct acg gga gtg ttc ggg acg gct tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tac gac aag atc atg ctg gac ggc cgt gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttc aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tca gat gcc gcg ctc atg gtt ctc cac tcc gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc ccn gga cac ttc cgt gac aca atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agt ccc gtc gtt ggt gtg gtc aac aac gcc gac ccn gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gac acc atg ccc gga ctc ttc gca tac cgc gcg ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 ggt tat tgt gga gca gcc gtt ctt gca aaa gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtc ggc acc cac tca gcc ggt ggc aac gga gta ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgt gtc tca cga tcc atg ctc ctg cag atg aaa gcc cac atc gac ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gcc ccc cac acc gag 639 Ala Pro His Thr Glu 210 <210> 356 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 356 Ser Gly Cys Pro Pro Thr Asp Leu Gln Arg Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 357 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/V124P_SAT_Egypt_2010 <400> 357 agt gga gcg cca ccc acc gac ttg caa aag atg gtg atg gcc aac acc 48 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 aaa cca gtc gag ctc ccn ctc gat ggt aag aca gtg gcg atc tgc tgt 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 gcc act gga gtg ttt ggg act gcc tat ctc gtg cct cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gct gag aag tat gac aag atc atg att gac ggc agg gcc atg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 cgt gat ttc aga gtg ttt gag ttt gag att aaa gta aaa gga cag gac 240 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tcg gac gcc gcc ctc atg gtg ctg cac cgt ggg aac cgc gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 aga gac atc ccn aag cac ttt cgt gat caa gca aga atg aga aaa gga 336 Arg Asp Ile Pro Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 acc ccc gtg gtt ggc gtg atc aac aac gcc gac ccn ggg aga ctc atc 384 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tct gga gag gca ctc acc tac aaa gac att gta gtg tgt atg gat 432 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 ggc gac acc atg cca ggc ctc ttt gcc tat aaa gcc gcc acc aaa gct 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 ggc tac tgt gga gga gcc gtt ctt gcg aaa gac gga gcc gag act ttc 528 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 atc gtc ggc act cac tcc gct gga gga aac gga gtt ggt tac tgc tct 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtt tcc aag tcc atg ctc ctg caa atg aag gca cac att gat cct 624 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gaa cca cac cac gaa 639 Glu Pro His His Glu 210 <210> 358 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 358 Ser Gly Ala Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Thr 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Ile Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Ile Asp Gly Arg Ala Met Thr Asp 50 55 60 Arg Asp Phe Arg Val Phe Glu Phe Glu Ile Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Arg Gly Asn Arg Val 85 90 95 Arg Asp Ile Pro Lys His Phe Arg Asp Gln Ala Arg Met Arg Lys Gly 100 105 110 Thr Pro Val Val Gly Val Ile Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Glu Ala Leu Thr Tyr Lys Asp Ile Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Lys Ala Ala Thr Lys Ala 145 150 155 160 Gly Tyr Cys Gly Gly Ala Val Leu Ala Lys Asp Gly Ala Glu Thr Phe 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Lys Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Glu Pro His His Glu 210 <210> 359 <211> 639 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(639) <220> <221> misc_feature <222> (1)..(639) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(639) <223> I22P/T100P/V124P_SAT3 <400> 359 agc gga tgc cca ccg acc gac ttg caa aag atg gtc atg gca aac gta 48 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 aag cct gtt gag ctc ccn ctc gac ggg aag acc gtt gcg ctc tgc tgc 96 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 gcg acg gga gtg ttt ggg aca gct tac ctc gtg ccc cgt cat ctt ttc 144 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 gca gag aag tat gac aag atc atg ctg gac ggc cgc gcc ctg aca gac 192 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 agt gac ttt aga gtg ttt gag ttt gag gtg aaa gta aaa gga cag gac 240 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 atg ctc tct gat gcc gca ctc atg gtt ctc cac tct gga aac cgt gtg 288 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 cgc gat ctc ccn gga cac ttc cgt gac acc atg aaa ctg tcg aaa ggc 336 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 agc ccc gtc gtt ggt gtt gtc aac aac gct gat ccn gga aga ctc atc 384 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 ttc tca gga gac gca ctg acc tac aaa gac ctg gtc gtt tgt atg gac 432 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 ggt gat acc atg cct gga ctc ttc gct tac cgc gca ggg acc aag gtt 480 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 gga tac tgc gga gct gcc gtt ctc gca aag gac ggc gcc aag act gtg 528 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 atc gtt ggc acc cac tca gcc gga ggc aac gga gtg ggc tac tgc tcc 576 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 tgc gtc tca cga tcc atg ctc ctg cag atg aag gcc cac att gat ccg 624 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 gct cct cac acc gag 639 Ala Pro His Thr Glu 210 <210> 360 <211> 213 <212> PRT <213> Foot-and-mouth disease virus <400> 360 Ser Gly Cys Pro Pro Thr Asp Leu Gln Lys Met Val Met Ala Asn Val 1 5 10 15 Lys Pro Val Glu Leu Pro Leu Asp Gly Lys Thr Val Ala Leu Cys Cys 20 25 30 Ala Thr Gly Val Phe Gly Thr Ala Tyr Leu Val Pro Arg His Leu Phe 35 40 45 Ala Glu Lys Tyr Asp Lys Ile Met Leu Asp Gly Arg Ala Leu Thr Asp 50 55 60 Ser Asp Phe Arg Val Phe Glu Phe Glu Val Lys Val Lys Gly Gln Asp 65 70 75 80 Met Leu Ser Asp Ala Ala Leu Met Val Leu His Ser Gly Asn Arg Val 85 90 95 Arg Asp Leu Pro Gly His Phe Arg Asp Thr Met Lys Leu Ser Lys Gly 100 105 110 Ser Pro Val Val Gly Val Val Asn Asn Ala Asp Pro Gly Arg Leu Ile 115 120 125 Phe Ser Gly Asp Ala Leu Thr Tyr Lys Asp Leu Val Val Cys Met Asp 130 135 140 Gly Asp Thr Met Pro Gly Leu Phe Ala Tyr Arg Ala Gly Thr Lys Val 145 150 155 160 Gly Tyr Cys Gly Ala Ala Val Leu Ala Lys Asp Gly Ala Lys Thr Val 165 170 175 Ile Val Gly Thr His Ser Ala Gly Gly Asn Gly Val Gly Tyr Cys Ser 180 185 190 Cys Val Ser Arg Ser Met Leu Leu Gln Met Lys Ala His Ile Asp Pro 195 200 205 Ala Pro His Thr Glu 210 <210> 361 <211> 2262 <212> DNA <213> Artificial Sequence <220> <223> P1_A2006_cut_sites_removed <400> 361 ggagccgggc aatccagtcc ggcaaccggg tcacaaaacc aatcaggcaa cactggtagt 60 atcatcaaca actactacat gcagcagtac cagaactcca tggatacaca acttggcgac 120 aacgccatta gcggtggttc caacgagggc tccactgaca ctacctccac acacacaacc 180 aacacacaga acaatgactg gttttcaaag ctggccagtt ctgccttcag cggtctcttc 240 ggcgctcttc tcgctgacaa aaagacagag gagactaccc tcctggaaga ccgcatcctt 300 accacccgca acggacacac cacctcgaca acccagtcga gtgtgggtgt cacctacggg 360 tactccactg gtgaagacca cgtctctgga cctaacacat ctggcctgga gacgcgagtg 420 gtacaggcag agagattctt caagaaacac ttgtttgatt ggacaactga taaggctttt 480 ggacacctgg aaaaactgga actccccacc gaacacaagg gtgtctatgg gcacttggtg 540 gactctttcg catacatgag aaatggctgg gacgtggagg tgacagccgt tggcaatcag 600 ttcaacggtg ggtgtctcct ggtggccatg gttcctgagt ggaaagagtt tacccctcgt 660 gagaaatacc agctcaccct gtttccacac caatttatca accccagaac caacatgaca 720 gcccacatca cggtcccgta ccttggtgtc aataggtatg accagtacaa acagcacaaa 780 ccctggacac tggtcgtgat ggtggtttcg ccactgacca ccagcagcat tggggcctca 840 cagattaagg tctatgccaa cattgcccca accttcgttc acgtggccgg cgagctccca 900 tcgaaagaag ggatcgtgcc ggttgcttgt acagacgggt acggtggcct ggtgacaaca 960 gacccgaaaa cagctgaccc tgtttatggt atggtgtaca acccgcccag aaccaactac 1020 cctgggcgct ttacaaactt gttggacgtg gccgaggctt gcccgacctt cctctgtttt 1080 gacgacggga aaccttacgt tgtgacaagg acggacgacc aacgcctcct ggccaagttt 1140 gacgtttctc ttgctgcaaa gcacatgtca aacacctacc tctcagggat agcacagtac 1200 tacacgcagt actctggcac tatcaatctg catttcatgt tcactggctc tactgaatca 1260 aaggcccggt acatggtggc gtacattcca cctggcatgg acaacccacc ggacacacct 1320 gagaaggctg cacactgcat ccacgccgag tgggacaccg ggctgaactc caaatttact 1380 ttttctatcc cttacgtgtc tgctgcagac tacgcataca ctgcgtctga cgtggcagaa 1440 acaacaaacg tacaggggtg ggtctgcata taccaaatca ctcacgggaa ggctgaacag 1500 gacactctgg tcgtgtcggt cagcgccggc aaggactttg aactgcgcct cccaattgac 1560 ccccgcacgc aaaccaccac tgccggggag tcagcagacc ctgtcaccac caccgttgag 1620 aactacggtg gtgagacaca ggctcagcga cgtcagcaca ctgacgtcgg cttcatcatg 1680 gacaggtttg cgaaaatcag ccccgtgagc cccacgcacg tcattgacct catgcaaaca 1740 caccaacatg cgttggtggg tgcccttttg cgtgcagcca cgtactactt ctccgatctg 1800 gagattgtgg tgcgtcatga tggcaacttg acgtgggtgc ccaatggagc acctgtagaa 1860 gccttggcca acacaagcaa ccccaccgcc taccacaagc agccatttac gagacttgcg 1920 ctcccttaca ccgcgccgca ccgagtgttg gcaacagtgt ataacggagt aagcaagtac 1980 tctacaactg gtaatggcag aaggggtgat ctggggcctc ttgcggcgcg ggtcgccgca 2040 cagctcccca gctctttcaa ttttggtgca attcgggcca cgaccgtcca cgagcttctc 2100 gtgcgcatga aacgtgccga gctctactgt ccaaggcctc tgctggcagt ggaagtgttg 2160 tcgcaggaca gacacaagca aaagatcatt gcacctgcaa agcaactcct gaacttcgac 2220 ctgctcaagt tggcgggaga cgttgagtcc aaccccgggc cc 2262 <210> 362 <211> 2262 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(2262) <220> <221> misc_feature <222> (1)..(2262) <223> P1_A2006_wildtype <400> 362 gga gcc ggg caa tcc agt ccg gca acc ggg tca caa aac caa tca ggc 48 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 aac act ggt agt atc atc aac aac tac tac atg cag cag tac cag aac 96 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 tcc atg gat aca caa ctt ggc gac aac gcc att agc ggt ggt tcc aac 144 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 gag ggc tcc act gac act acc tcc aca cac aca acc aac aca cag aac 192 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Thr Asn Thr Gln Asn 50 55 60 aat gac tgg ttt tca aag ctg gcc agt tct gcc ttc agc ggt ctc ttc 240 Asn Asp Trp Phe Ser Lys Leu Ala Ser Ser Ala Phe Ser Gly Leu Phe 65 70 75 80 ggc gct ctt ctc gct gac aaa aag aca gag gag act acc ctc ctg gag 288 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 gac cgc atc ctt acc acc cgc aac gga cac acc acc tcg aca acc cag 336 Asp Arg Ile Leu Thr Thr Arg Asn Gly His Thr Thr Ser Thr Thr Gln 100 105 110 tcg agt gtg ggt gtc acc tac ggg tac tcc act ggt gaa gac cac gtc 384 Ser Ser Val Gly Val Thr Tyr Gly Tyr Ser Thr Gly Glu Asp His Val 115 120 125 tct gga cct aac aca tct ggc ctg gag acg cga gtg gta cag gca gag 432 Ser Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Val Gln Ala Glu 130 135 140 aga ttc ttc aag aaa cac ttg ttt gat tgg aca act gat aaa gct ttt 480 Arg Phe Phe Lys Lys His Leu Phe Asp Trp Thr Thr Asp Lys Ala Phe 145 150 155 160 gga cac ctg gaa aaa ctg gaa ctc ccc acc gaa cac aag ggt gtc tac 528 Gly His Leu Glu Lys Leu Glu Leu Pro Thr Glu His Lys Gly Val Tyr 165 170 175 ggg cac ttg gtg gac tct ttc gca tac atg aga aat ggc tgg gac gtg 576 Gly His Leu Val Asp Ser Phe Ala Tyr Met Arg Asn Gly Trp Asp Val 180 185 190 gag gtg acc gcc gtt ggc aac cag ttc aac ggt ggg tgt ctc ctg gtg 624 Glu Val Thr Ala Val Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu Val 195 200 205 gcc atg gta cct gag tgg aaa gag ttt acc cct cgt gag aaa tac cag 672 Ala Met Val Pro Glu Trp Lys Glu Phe Thr Pro Arg Glu Lys Tyr Gln 210 215 220 ctc acc ctg ttt cca cac caa ttt atc aac ccc aga acc aac atg aca 720 Leu Thr Leu Phe Pro His Gln Phe Ile Asn Pro Arg Thr Asn Met Thr 225 230 235 240 gcc cac atc acg gtc ccg tac ctt ggt gtc aat agg tat gac cag tac 768 Ala His Ile Thr Val Pro Tyr Leu Gly Val Asn Arg Tyr Asp Gln Tyr 245 250 255 aaa cag cac aaa ccc tgg aca ctg gtc gtg atg gtg gtt tcg cca ctg 816 Lys Gln His Lys Pro Trp Thr Leu Val Val Met Val Val Ser Pro Leu 260 265 270 acc acc agc agc att ggg gcc tca cag att aag gtc tac gcc aac att 864 Thr Thr Ser Ser Ile Gly Ala Ser Gln Ile Lys Val Tyr Ala Asn Ile 275 280 285 gcc cca acc ttc gtt cac gtg gcc ggc gag ctc cca tcg aaa gaa ggg 912 Ala Pro Thr Phe Val His Val Ala Gly Glu Leu Pro Ser Lys Glu Gly 290 295 300 atc gtg ccg gtt gct tgt aca gac ggg tac ggt ggc ctg gtg aca aca 960 Ile Val Pro Val Ala Cys Thr Asp Gly Tyr Gly Gly Leu Val Thr Thr 305 310 315 320 gac ccg aaa aca gct gac cct gtt tat ggt atg gtg tac aac ccg ccc 1008 Asp Pro Lys Thr Ala Asp Pro Val Tyr Gly Met Val Tyr Asn Pro Pro 325 330 335 aga acc aac tac cct ggg cgc ttt aca aac ttg ttg gac gtg gcc gag 1056 Arg Thr Asn Tyr Pro Gly Arg Phe Thr Asn Leu Leu Asp Val Ala Glu 340 345 350 gct tgc ccg acc ttc ctc tgt ttt gac gac ggg aaa ccg tac gtt gtg 1104 Ala Cys Pro Thr Phe Leu Cys Phe Asp Asp Gly Lys Pro Tyr Val Val 355 360 365 aca agg acg gac gac caa cgc ctc ctg gcc aag ttt gac gtt tct ctt 1152 Thr Arg Thr Asp Asp Gln Arg Leu Leu Ala Lys Phe Asp Val Ser Leu 370 375 380 gct gca aag cac atg tca aac acc tac ctc tca ggg ata gca cag tac 1200 Ala Ala Lys His Met Ser Asn Thr Tyr Leu Ser Gly Ile Ala Gln Tyr 385 390 395 400 tac acg cag tac tct ggc act atc aat ctg cat ttc atg ttc act ggc 1248 Tyr Thr Gln Tyr Ser Gly Thr Ile Asn Leu His Phe Met Phe Thr Gly 405 410 415 tct act gaa tca aag gcc cgg tac atg gtg gcg tac att cca cct ggc 1296 Ser Thr Glu Ser Lys Ala Arg Tyr Met Val Ala Tyr Ile Pro Pro Gly 420 425 430 atg gac aac cca ccg gac aca cct gag aag gct gca cac tgc atc cac 1344 Met Asp Asn Pro Pro Asp Thr Pro Glu Lys Ala Ala His Cys Ile His 435 440 445 gcc gag tgg gac acc ggg ctg aac tcc aaa ttt act ttt tct atc ccg 1392 Ala Glu Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro 450 455 460 tac gtg tct gct gca gac tac gca tac act gcg tct gac gtg gca gaa 1440 Tyr Val Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Asp Val Ala Glu 465 470 475 480 aca aca aac gta cag ggg tgg gtc tgc ata tac caa atc act cac ggg 1488 Thr Thr Asn Val Gln Gly Trp Val Cys Ile Tyr Gln Ile Thr His Gly 485 490 495 aag gct gaa cag gac act ctg gtc gtg tcg gtc agc gcc ggc aag gac 1536 Lys Ala Glu Gln Asp Thr Leu Val Val Ser Val Ser Ala Gly Lys Asp 500 505 510 ttt gaa ctg cgc ctc cca att gac ccc cgc acg caa acc acc act gcc 1584 Phe Glu Leu Arg Leu Pro Ile Asp Pro Arg Thr Gln Thr Thr Thr Ala 515 520 525 ggg gag tca gca gac cct gtc acc acc acc gtt gag aac tac ggt ggt 1632 Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu Asn Tyr Gly Gly 530 535 540 gag aca cag gct cag cga cgt cag cac act gac gtc ggc ttc atc atg 1680 Glu Thr Gln Ala Gln Arg Arg Gln His Thr Asp Val Gly Phe Ile Met 545 550 555 560 gac agg ttt gcg aaa atc agc ccc gtg agc ccc acg cac gtc att gac 1728 Asp Arg Phe Ala Lys Ile Ser Pro Val Ser Pro Thr His Val Ile Asp 565 570 575 ctc atg caa aca cac caa cac gcg ttg gtg ggt gcc ctt ttg cgt gca 1776 Leu Met Gln Thr His Gln His Ala Leu Val Gly Ala Leu Leu Arg Ala 580 585 590 gcc acg tac tac ttc tcc gat ctg gag att gtg gtg cgt cat gat ggc 1824 Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Ile Val Val Arg His Asp Gly 595 600 605 aac ttg acg tgg gtg ccc aat gga gca cct gta gaa gcc ttg gcc aac 1872 Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Val Glu Ala Leu Ala Asn 610 615 620 aca agc aac ccc acc gcc tac cac aag cag cca ttt acg aga ctt gcg 1920 Thr Ser Asn Pro Thr Ala Tyr His Lys Gln Pro Phe Thr Arg Leu Ala 625 630 635 640 ctc cct tac acc gcg ccg cac cga gtg ttg gca aca gtg tat aac gga 1968 Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr Val Tyr Asn Gly 645 650 655 gta agc aag tac tct aca act ggt aat ggc aga agg ggt gac ctg ggg 2016 Val Ser Lys Tyr Ser Thr Thr Gly Asn Gly Arg Arg Gly Asp Leu Gly 660 665 670 cct ctt gcg gcg cgg gtc gcc gca cag ctc ccc agc tct ttc aat ttt 2064 Pro Leu Ala Ala Arg Val Ala Ala Gln Leu Pro Ser Ser Phe Asn Phe 675 680 685 ggt gca att cgg gcc acg acc gtc cac gag ctt ctc gtg cgc atg aaa 2112 Gly Ala Ile Arg Ala Thr Thr Val His Glu Leu Leu Val Arg Met Lys 690 695 700 cgt gcc gag ctc tac tgt ccc agg cct ctg ctg gca gtg gaa gtg ttg 2160 Arg Ala Glu Leu Tyr Cys Pro Arg Pro Leu Leu Ala Val Glu Val Leu 705 710 715 720 tcg cag gac aga cac aag caa aag atc att gca cct gca aag caa ctc 2208 Ser Gln Asp Arg His Lys Gln Lys Ile Ile Ala Pro Ala Lys Gln Leu 725 730 735 ctg aac ttc gac ctg ctc aag ttg gcg gga gac gtt gag tcc aac cct 2256 Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 740 745 750 ggg ccc 2262 Gly Pro <210> 363 <211> 754 <212> PRT <213> Foot-and-mouth disease virus <400> 363 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Thr Asn Thr Gln Asn 50 55 60 Asn Asp Trp Phe Ser Lys Leu Ala Ser Ser Ala Phe Ser Gly Leu Phe 65 70 75 80 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 Asp Arg Ile Leu Thr Thr Arg Asn Gly His Thr Thr Ser Thr Thr Gln 100 105 110 Ser Ser Val Gly Val Thr Tyr Gly Tyr Ser Thr Gly Glu Asp His Val 115 120 125 Ser Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Val Gln Ala Glu 130 135 140 Arg Phe Phe Lys Lys His Leu Phe Asp Trp Thr Thr Asp Lys Ala Phe 145 150 155 160 Gly His Leu Glu Lys Leu Glu Leu Pro Thr Glu His Lys Gly Val Tyr 165 170 175 Gly His Leu Val Asp Ser Phe Ala Tyr Met Arg Asn Gly Trp Asp Val 180 185 190 Glu Val Thr Ala Val Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu Val 195 200 205 Ala Met Val Pro Glu Trp Lys Glu Phe Thr Pro Arg Glu Lys Tyr Gln 210 215 220 Leu Thr Leu Phe Pro His Gln Phe Ile Asn Pro Arg Thr Asn Met Thr 225 230 235 240 Ala His Ile Thr Val Pro Tyr Leu Gly Val Asn Arg Tyr Asp Gln Tyr 245 250 255 Lys Gln His Lys Pro Trp Thr Leu Val Val Met Val Val Ser Pro Leu 260 265 270 Thr Thr Ser Ser Ile Gly Ala Ser Gln Ile Lys Val Tyr Ala Asn Ile 275 280 285 Ala Pro Thr Phe Val His Val Ala Gly Glu Leu Pro Ser Lys Glu Gly 290 295 300 Ile Val Pro Val Ala Cys Thr Asp Gly Tyr Gly Gly Leu Val Thr Thr 305 310 315 320 Asp Pro Lys Thr Ala Asp Pro Val Tyr Gly Met Val Tyr Asn Pro Pro 325 330 335 Arg Thr Asn Tyr Pro Gly Arg Phe Thr Asn Leu Leu Asp Val Ala Glu 340 345 350 Ala Cys Pro Thr Phe Leu Cys Phe Asp Asp Gly Lys Pro Tyr Val Val 355 360 365 Thr Arg Thr Asp Asp Gln Arg Leu Leu Ala Lys Phe Asp Val Ser Leu 370 375 380 Ala Ala Lys His Met Ser Asn Thr Tyr Leu Ser Gly Ile Ala Gln Tyr 385 390 395 400 Tyr Thr Gln Tyr Ser Gly Thr Ile Asn Leu His Phe Met Phe Thr Gly 405 410 415 Ser Thr Glu Ser Lys Ala Arg Tyr Met Val Ala Tyr Ile Pro Pro Gly 420 425 430 Met Asp Asn Pro Pro Asp Thr Pro Glu Lys Ala Ala His Cys Ile His 435 440 445 Ala Glu Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro 450 455 460 Tyr Val Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Asp Val Ala Glu 465 470 475 480 Thr Thr Asn Val Gln Gly Trp Val Cys Ile Tyr Gln Ile Thr His Gly 485 490 495 Lys Ala Glu Gln Asp Thr Leu Val Val Ser Val Ser Ala Gly Lys Asp 500 505 510 Phe Glu Leu Arg Leu Pro Ile Asp Pro Arg Thr Gln Thr Thr Thr Ala 515 520 525 Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu Asn Tyr Gly Gly 530 535 540 Glu Thr Gln Ala Gln Arg Arg Gln His Thr Asp Val Gly Phe Ile Met 545 550 555 560 Asp Arg Phe Ala Lys Ile Ser Pro Val Ser Pro Thr His Val Ile Asp 565 570 575 Leu Met Gln Thr His Gln His Ala Leu Val Gly Ala Leu Leu Arg Ala 580 585 590 Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Ile Val Val Arg His Asp Gly 595 600 605 Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Val Glu Ala Leu Ala Asn 610 615 620 Thr Ser Asn Pro Thr Ala Tyr His Lys Gln Pro Phe Thr Arg Leu Ala 625 630 635 640 Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr Val Tyr Asn Gly 645 650 655 Val Ser Lys Tyr Ser Thr Thr Gly Asn Gly Arg Arg Gly Asp Leu Gly 660 665 670 Pro Leu Ala Ala Arg Val Ala Ala Gln Leu Pro Ser Ser Phe Asn Phe 675 680 685 Gly Ala Ile Arg Ala Thr Thr Val His Glu Leu Leu Val Arg Met Lys 690 695 700 Arg Ala Glu Leu Tyr Cys Pro Arg Pro Leu Leu Ala Val Glu Val Leu 705 710 715 720 Ser Gln Asp Arg His Lys Gln Lys Ile Ile Ala Pro Ala Lys Gln Leu 725 730 735 Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 740 745 750 Gly Pro <210> 364 <211> 2262 <212> DNA <213> Artificial Sequence <220> <223> P1_A24_Cruzeiro_cut_sites_removed <400> 364 ggggccgggc aatccagtcc ggcgaccggc tcgcagaacc aatctggcaa cactggcagc 60 ataattaaca actactacat gcagcaatac cagaactcca tggacacaca gttgggagac 120 aatgccatca gtggaggctc caacgagggc tccacggaca caacttcaac acacacaacc 180 aacactcaaa acaatgactg gttctcgaag ctcgccagtt cagcttttac aggtctgttc 240 ggtgcactgc tcgccgacaa gaagacagag gaaacgacac ttcttgagga ccgcatcctc 300 accacccgca acgggcacac cacctcgacg acccaatcga gtgtgggtgt cacacacggg 360 tactccacag aggaggacca cgttgctggg ccaaacacat cgggcctgga gacgcgagtg 420 gtgcaggcag agagattcta caaaaagtac ttgtttgact ggacaacgga caaggcattt 480 ggacacctgg aaaagctgga gctcccgtcc gaccaccacg gtgtctttgg acacttggtg 540 gactcgtacg cctatatgag aaatggctgg gatgttgagg tgtccgctgt tggcaaccag 600 ttcaacggcg ggtgcctcct ggtggccatg gttcctgaat ggaaggaatt tgacacacgg 660 gagaaatacc aactcaccct tttcccgcac cagtttatta gccccagaac taacatgact 720 gcccacatca cggtccccta ccttggtgtg aacaggtatg atcagtacaa gaagcataag 780 ccctggacat tggttgtcat ggtcgtgtcg ccacttacgg tcaacaacac cagtgcggca 840 caaatcaagg tctatgccaa catagctccg acctatgttc acgtggccgg tgaactcccc 900 tcgaaagagg ggattttccc ggttgcatgt gcggacggtt acggaggatt ggtgacgaca 960 gacccgaaga cagctgaccc tgcttatggc aaggtgtaca acccgcctag gactaactac 1020 cctgggcgct tcaccaacct gttggacgtg gccgaagcgt gtcccacttt cctctgcttt 1080 gacgacggga aaccgtacgt caccacgcgg acggatgaca cccgactttt ggccaagttt 1140 gacctttccc ttgccgcaaa acacatgtcc aacacatacc tgtcagggat tgctcagtac 1200 tacacacagt actctggcac catcaatttg catttcatgt tcacaggttc cactgattca 1260 aaggcccgat acatggtggc ctacatccca cctggggtgg agacaccacc ggacacacct 1320 gaaagggctg cccactgcat tcacgctgaa tgggacactg gactaaactc caaattcact 1380 ttctcaatcc cgtacgtatc cgccgccgat tatgcgtaca cagcgtctga cacggcagaa 1440 acaatcaacg tacagggatg ggtctgcatc taccaaatta cacacgggaa ggctgaaaat 1500 gacaccttgg tcgtgtcggt tagcgccggc aaagactttg agttgcgcct cccgattgac 1560 ccccgccagc agaccaccgc taccggggaa tcagcagacc cggtcacaac caccgtggag 1620 aactacggcg gtgagacaca aatccagaga cgtcaccaca cggacattgg tttcatcatg 1680 gacagatttg tgaagatcca gagcttgagc ccaacacatg tcattgacct catgcagact 1740 caccaacacg gtctggtggg tgccttgctg cgtgcagcca cgtactactt ttctgacctg 1800 gaaattgttg tacggcacga aggcaatctg acctgggtgc ccaacggcgc ccctgaatca 1860 gccctgttga acaccagcaa ccccactgcc tacaacaagg caccattcac gagactcgct 1920 ctcccctaca ctgcgccgca ccgtgtgctg gcaacagtgt acaacgggac gagtaagtat 1980 gctgtgggtg gttcaggcag aagaggcgac atggggtctc tcgcggcgcg agtcgtgaaa 2040 cagcttcctg cttcatttaa ctacggtgca atcaaggccg acgccatcca cgaacttctc 2100 gtgcgcatga aacgggccga gctctactgc cccagaccgc tgttggcaat agaggtgtct 2160 tcgcaagaca ggcacaagca aaagatcatt gcaccagcaa agcagcttct gaattttgac 2220 ctgctcaagt tggccggaga cgttgagtcc aaccctgggc cc 2262 <210> 365 <211> 2262 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(2262) <220> <221> misc_feature <222> (1)..(2262) <223> P1_A24_Cruzeiro_wild_type <400> 365 ggg gcc ggg caa tcc agt ccg gcg acc ggc tcg cag aac caa tct ggc 48 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 aac act ggc agc ata att aac aac tac tac atg cag caa tac cag aac 96 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 tcc atg gac aca cag ttg gga gac aat gcc atc agt gga ggc tcc aac 144 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 gag ggc tcc acg gac aca act tca aca cac aca acc aac act caa aac 192 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Thr Asn Thr Gln Asn 50 55 60 aat gac tgg ttc tcg aag ctc gcc agt tca gct ttt acc ggt ctg ttc 240 Asn Asp Trp Phe Ser Lys Leu Ala Ser Ser Ala Phe Thr Gly Leu Phe 65 70 75 80 ggt gca ctg ctc gcc gac aag aag aca gag gaa acg aca ctt ctt gag 288 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 gac cgc atc ctc acc acc cgc aac ggg cac acc acc tcg acg acc caa 336 Asp Arg Ile Leu Thr Thr Arg Asn Gly His Thr Thr Ser Thr Thr Gln 100 105 110 tcg agt gtg ggt gtc aca cac ggg tac tcc aca gag gag gac cac gtt 384 Ser Ser Val Gly Val Thr His Gly Tyr Ser Thr Glu Glu Asp His Val 115 120 125 gct ggg ccc aac aca tcg ggc ctg gag acg cga gtg gtg cag gca gag 432 Ala Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Val Gln Ala Glu 130 135 140 aga ttc tac aaa aag tac ttg ttt gac tgg aca acg gac aag gca ttt 480 Arg Phe Tyr Lys Lys Tyr Leu Phe Asp Trp Thr Thr Asp Lys Ala Phe 145 150 155 160 gga cac ctg gaa aag ctg gag ctc ccg tcc gac cac cac ggt gtc ttt 528 Gly His Leu Glu Lys Leu Glu Leu Pro Ser Asp His His Gly Val Phe 165 170 175 gga cac ttg gtg gac tcg tac gcc tat atg aga aat ggc tgg gat gtt 576 Gly His Leu Val Asp Ser Tyr Ala Tyr Met Arg Asn Gly Trp Asp Val 180 185 190 gag gtg tcc gct gtt ggc aac cag ttc aac ggc ggg tgc ctc ctg gtg 624 Glu Val Ser Ala Val Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu Val 195 200 205 gcc atg gta cct gaa tgg aag gaa ttt gac aca cgg gag aaa tac caa 672 Ala Met Val Pro Glu Trp Lys Glu Phe Asp Thr Arg Glu Lys Tyr Gln 210 215 220 ctc acc ctt ttc ccg cac cag ttt att agc ccc aga act aac atg act 720 Leu Thr Leu Phe Pro His Gln Phe Ile Ser Pro Arg Thr Asn Met Thr 225 230 235 240 gcc cac atc acg gtc ccc tac ctt ggt gtg aac agg tat gat cag tac 768 Ala His Ile Thr Val Pro Tyr Leu Gly Val Asn Arg Tyr Asp Gln Tyr 245 250 255 aag aag cat aag ccc tgg aca ttg gtt gtc atg gtc gtg tcg cca ctt 816 Lys Lys His Lys Pro Trp Thr Leu Val Val Met Val Val Ser Pro Leu 260 265 270 acg gtc aac aac act agt gcg gca caa atc aag gtc tac gcc aac ata 864 Thr Val Asn Asn Thr Ser Ala Ala Gln Ile Lys Val Tyr Ala Asn Ile 275 280 285 gct ccg acc tat gtt cac gtg gcc ggt gaa ctc ccc tcg aaa gag ggg 912 Ala Pro Thr Tyr Val His Val Ala Gly Glu Leu Pro Ser Lys Glu Gly 290 295 300 att ttc ccg gtt gca tgt gcg gac ggt tac gga gga ttg gtg acg aca 960 Ile Phe Pro Val Ala Cys Ala Asp Gly Tyr Gly Gly Leu Val Thr Thr 305 310 315 320 gac ccg aag aca gct gac cct gct tat ggc aag gtg tac aac ccg cct 1008 Asp Pro Lys Thr Ala Asp Pro Ala Tyr Gly Lys Val Tyr Asn Pro Pro 325 330 335 agg act aac tac cct ggg cgc ttc acc aac ctg ttg gac gtg gcc gaa 1056 Arg Thr Asn Tyr Pro Gly Arg Phe Thr Asn Leu Leu Asp Val Ala Glu 340 345 350 gcg tgt ccc act ttc ctc tgc ttt gac gac ggg aaa ccg tac gtc acc 1104 Ala Cys Pro Thr Phe Leu Cys Phe Asp Asp Gly Lys Pro Tyr Val Thr 355 360 365 acg cgg acg gat gac acc cga ctt ttg gcc aag ttt gac ctt tcc ctt 1152 Thr Arg Thr Asp Asp Thr Arg Leu Leu Ala Lys Phe Asp Leu Ser Leu 370 375 380 gcc gca aaa cat atg tcc aac aca tac ctg tca ggg att gct cag tac 1200 Ala Ala Lys His Met Ser Asn Thr Tyr Leu Ser Gly Ile Ala Gln Tyr 385 390 395 400 tac aca cag tac tct ggc acc atc aat ttg cat ttc atg ttc aca ggt 1248 Tyr Thr Gln Tyr Ser Gly Thr Ile Asn Leu His Phe Met Phe Thr Gly 405 410 415 tcc act gat tca aag gcc cga tac atg gtg gcc tac atc cca cct ggg 1296 Ser Thr Asp Ser Lys Ala Arg Tyr Met Val Ala Tyr Ile Pro Pro Gly 420 425 430 gtg gag aca cca ccg gac aca cct gaa agg gct gcc cac tgc att cac 1344 Val Glu Thr Pro Pro Asp Thr Pro Glu Arg Ala Ala His Cys Ile His 435 440 445 gct gaa tgg gac act gga cta aac tcc aaa ttc act ttc tca atc ccg 1392 Ala Glu Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro 450 455 460 tac gta tcc gcc gcg gat tac gcg tac aca gcg tct gac acg gca gaa 1440 Tyr Val Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Asp Thr Ala Glu 465 470 475 480 aca atc aac gta cag gga tgg gtc tgc atc tac caa att aca cac ggg 1488 Thr Ile Asn Val Gln Gly Trp Val Cys Ile Tyr Gln Ile Thr His Gly 485 490 495 aag gct gaa aat gac acc ttg gtc gtg tcg gtt agc gcc ggc aaa gac 1536 Lys Ala Glu Asn Asp Thr Leu Val Val Ser Val Ser Ala Gly Lys Asp 500 505 510 ttt gag ttg cgc ctc ccg att gac ccc cgc cag cag acc acc gct acc 1584 Phe Glu Leu Arg Leu Pro Ile Asp Pro Arg Gln Gln Thr Thr Ala Thr 515 520 525 ggg gaa tca gca gac ccg gtc acc acc acc gtg gag aac tac ggc ggt 1632 Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu Asn Tyr Gly Gly 530 535 540 gag aca caa atc cag aga cgt cac cac acg gac att ggt ttc atc atg 1680 Glu Thr Gln Ile Gln Arg Arg His His Thr Asp Ile Gly Phe Ile Met 545 550 555 560 gac aga ttt gtg aag atc caa agc ttg agc cca aca cat gtc att gac 1728 Asp Arg Phe Val Lys Ile Gln Ser Leu Ser Pro Thr His Val Ile Asp 565 570 575 ctc atg cag act cac caa cac ggt ctg gtg ggt gcc ttg ctg cgt gca 1776 Leu Met Gln Thr His Gln His Gly Leu Val Gly Ala Leu Leu Arg Ala 580 585 590 gcc acg tac tac ttt tct gac ctg gaa att gtt gta cgg cac gaa ggc 1824 Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Ile Val Val Arg His Glu Gly 595 600 605 aat ctg acc tgg gtg ccc aac ggc gcc cct gaa tca gcc ctg ttg aac 1872 Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ser Ala Leu Leu Asn 610 615 620 acc agc aac ccc act gcc tac aac aag gca cca ttc acg aga ctc gct 1920 Thr Ser Asn Pro Thr Ala Tyr Asn Lys Ala Pro Phe Thr Arg Leu Ala 625 630 635 640 ctc ccc tac act gcg ccg cac cgt gtg ctg gca aca gtg tac aac ggg 1968 Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr Val Tyr Asn Gly 645 650 655 acg agt aag tat gct gtg ggt ggt tca ggc aga aga ggc gac atg ggg 2016 Thr Ser Lys Tyr Ala Val Gly Gly Ser Gly Arg Arg Gly Asp Met Gly 660 665 670 tct ctc gcg gcg cga gtc gtg aaa cag ctt cct gct tca ttt aac tac 2064 Ser Leu Ala Ala Arg Val Val Lys Gln Leu Pro Ala Ser Phe Asn Tyr 675 680 685 ggt gca atc aag gcc gac gcc atc cac gaa ctt ctc gtg cgc atg aaa 2112 Gly Ala Ile Lys Ala Asp Ala Ile His Glu Leu Leu Val Arg Met Lys 690 695 700 cgg gcc gag ctc tac tgc ccc aga ccg ctg ttg gca ata gag gtg tct 2160 Arg Ala Glu Leu Tyr Cys Pro Arg Pro Leu Leu Ala Ile Glu Val Ser 705 710 715 720 tcg caa gac agg cac aag caa aag atc att gca cca gca aag cag ctt 2208 Ser Gln Asp Arg His Lys Gln Lys Ile Ile Ala Pro Ala Lys Gln Leu 725 730 735 ctg aat ttt gac ctg ctc aag ttg gcc gga gac gtt gag tcc aac cct 2256 Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 740 745 750 ggg ccc 2262 Gly Pro <210> 366 <211> 754 <212> PRT <213> Foot-and-mouth disease virus <400> 366 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Thr Asn Thr Gln Asn 50 55 60 Asn Asp Trp Phe Ser Lys Leu Ala Ser Ser Ala Phe Thr Gly Leu Phe 65 70 75 80 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 Asp Arg Ile Leu Thr Thr Arg Asn Gly His Thr Thr Ser Thr Thr Gln 100 105 110 Ser Ser Val Gly Val Thr His Gly Tyr Ser Thr Glu Glu Asp His Val 115 120 125 Ala Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Val Gln Ala Glu 130 135 140 Arg Phe Tyr Lys Lys Tyr Leu Phe Asp Trp Thr Thr Asp Lys Ala Phe 145 150 155 160 Gly His Leu Glu Lys Leu Glu Leu Pro Ser Asp His His Gly Val Phe 165 170 175 Gly His Leu Val Asp Ser Tyr Ala Tyr Met Arg Asn Gly Trp Asp Val 180 185 190 Glu Val Ser Ala Val Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu Val 195 200 205 Ala Met Val Pro Glu Trp Lys Glu Phe Asp Thr Arg Glu Lys Tyr Gln 210 215 220 Leu Thr Leu Phe Pro His Gln Phe Ile Ser Pro Arg Thr Asn Met Thr 225 230 235 240 Ala His Ile Thr Val Pro Tyr Leu Gly Val Asn Arg Tyr Asp Gln Tyr 245 250 255 Lys Lys His Lys Pro Trp Thr Leu Val Val Met Val Val Ser Pro Leu 260 265 270 Thr Val Asn Asn Thr Ser Ala Ala Gln Ile Lys Val Tyr Ala Asn Ile 275 280 285 Ala Pro Thr Tyr Val His Val Ala Gly Glu Leu Pro Ser Lys Glu Gly 290 295 300 Ile Phe Pro Val Ala Cys Ala Asp Gly Tyr Gly Gly Leu Val Thr Thr 305 310 315 320 Asp Pro Lys Thr Ala Asp Pro Ala Tyr Gly Lys Val Tyr Asn Pro Pro 325 330 335 Arg Thr Asn Tyr Pro Gly Arg Phe Thr Asn Leu Leu Asp Val Ala Glu 340 345 350 Ala Cys Pro Thr Phe Leu Cys Phe Asp Asp Gly Lys Pro Tyr Val Thr 355 360 365 Thr Arg Thr Asp Asp Thr Arg Leu Leu Ala Lys Phe Asp Leu Ser Leu 370 375 380 Ala Ala Lys His Met Ser Asn Thr Tyr Leu Ser Gly Ile Ala Gln Tyr 385 390 395 400 Tyr Thr Gln Tyr Ser Gly Thr Ile Asn Leu His Phe Met Phe Thr Gly 405 410 415 Ser Thr Asp Ser Lys Ala Arg Tyr Met Val Ala Tyr Ile Pro Pro Gly 420 425 430 Val Glu Thr Pro Pro Asp Thr Pro Glu Arg Ala Ala His Cys Ile His 435 440 445 Ala Glu Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro 450 455 460 Tyr Val Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Asp Thr Ala Glu 465 470 475 480 Thr Ile Asn Val Gln Gly Trp Val Cys Ile Tyr Gln Ile Thr His Gly 485 490 495 Lys Ala Glu Asn Asp Thr Leu Val Val Ser Val Ser Ala Gly Lys Asp 500 505 510 Phe Glu Leu Arg Leu Pro Ile Asp Pro Arg Gln Gln Thr Thr Ala Thr 515 520 525 Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu Asn Tyr Gly Gly 530 535 540 Glu Thr Gln Ile Gln Arg Arg His His Thr Asp Ile Gly Phe Ile Met 545 550 555 560 Asp Arg Phe Val Lys Ile Gln Ser Leu Ser Pro Thr His Val Ile Asp 565 570 575 Leu Met Gln Thr His Gln His Gly Leu Val Gly Ala Leu Leu Arg Ala 580 585 590 Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Ile Val Val Arg His Glu Gly 595 600 605 Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ser Ala Leu Leu Asn 610 615 620 Thr Ser Asn Pro Thr Ala Tyr Asn Lys Ala Pro Phe Thr Arg Leu Ala 625 630 635 640 Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr Val Tyr Asn Gly 645 650 655 Thr Ser Lys Tyr Ala Val Gly Gly Ser Gly Arg Arg Gly Asp Met Gly 660 665 670 Ser Leu Ala Ala Arg Val Val Lys Gln Leu Pro Ala Ser Phe Asn Tyr 675 680 685 Gly Ala Ile Lys Ala Asp Ala Ile His Glu Leu Leu Val Arg Met Lys 690 695 700 Arg Ala Glu Leu Tyr Cys Pro Arg Pro Leu Leu Ala Ile Glu Val Ser 705 710 715 720 Ser Gln Asp Arg His Lys Gln Lys Ile Ile Ala Pro Ala Lys Gln Leu 725 730 735 Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 740 745 750 Gly Pro <210> 367 <211> 2249 <212> DNA <213> Artificial Sequence <220> <223> P1_Asia1_Shamir_cut_sites_removed <400> 367 ggagccggtc aatccagtcc ggcaaccggg tcacagaacc aatctggcaa cactggaagc 60 atcattaaca actactacat gcaacagtac cagaactcaa tggacacaca gcttggtgac 120 aacgctatta gcggaggttc caacgaaggt tccacggata ccacttccac acacacaaac 180 aacacccaaa acaacgactg gttctcgcgc ctggcaagct ctgcattcag tggactattt 240 ggtgcacttt tggctgacaa gaagacagaa gagacaactc tgcttgaaga ccgcattctc 300 accaccagga acggccacac aacatcgacg acacagtcga gcgttggcgt aacatacggt 360 tacgctgtgg ccgaggacgc ggtgtctgga cccaatacct cgggactcga gactcgtgtt 420 caacaggcag aacggttttt caagaaacac ctgtttgact ggacaccgaa cttggcattt 480 ggacactgtt actacctgga acttcccact gaacacaaag gcgtgtacgg cagtctcatg 540 ggctcatacg cctacatgag aaatggatgg gacatagagg tgactgctgt tggaaaccaa 600 ttcaacggtg gttgtctcct tgtcgcgctc gtgccagagc tgaaggaact cgacacgcga 660 cagaagtacc aactgaccct ctttccccac cagttcatca acccacgcac caacatgacg 720 gcccacatca acgtgccata cgtgggtatc aacaggtacg accagtacgc cctccacaag 780 ccgtggacgc ttgttgtgat ggtggtagcc ccactcaccg tcaaaactgg tggttctgaa 840 cagatcaagg tttacatgaa tgcagcgcca acctacgtgc atgtggcggg agagctgccc 900 tcgaaagagg gaatagttcc cgtcgcgtgt gcggacggtt acggcaacat ggtgacaacg 960 gacccgaaga cggccgatcc agtttacggg aaagtgttca acccccccag gacaaacctc 1020 cctgggcgct tcacgaactt ccttgatgtt gcggaggcat gtccaacttt cctccgcttt 1080 ggagaagtac catttgtgaa gacggtgaac tctggagacc gcttgctggc caagttcgac 1140 gtgtccctcg cagcagggca catgtccaac acctacttgg ctggcctggc gcagtactac 1200 acacagtaca gcggcaccat gaacgtccac ttcatgttca ccggacccac ggatgctaaa 1260 gcccgataca tggtggctta tgtcccccct ggcatgacac cgcccacgga ccctgagcac 1320 gccgcacact gcattcactc tgagtgggat actggtctta actctaagtt taccttttcc 1380 ataccttacc tctctgctgc tgactatgcc tacactgctt ctgacgtggc ggagaccacg 1440 agtgtgcagg gatgggtgtg tatctatcag atcacccacg gcaaggctga gggagacgca 1500 ctggtcgttt ctgtcagcgc cggcaaagac tttgagtttc gcttgcctgt tgacgcacgc 1560 cagcaaacca ccaccactgg cgaatcagca gatccagtca caaccacggt tgagaactat 1620 ggaggagaga ctcagacagc cagacggctt cacactgacg tcgccttcat tcttgacagg 1680 tttgtgaaac tcactgctcc caagaacatc caaaccctcg atctcatgca gatcccctca 1740 cacacgctgg ttggagcact acttcgttct gcgacgtact acttctcaga cctggaggtc 1800 gcgcttgtcc acacaggccc agtcacctgg gtgcccaacg gagcgcccaa ggatgctcta 1860 aacaaccaga ccaacccaac tgcctatcag aagcaaccca tcacccgcct ggcactcccc 1920 tacaccgccc cccatcgtgt gctggcaaca gtgtacaacg ggaagacggc ttacggggaa 1980 acgacctcaa ggcgcggcga catggcggcc ctcgcacaaa ggttgagcgc tcggctgccc 2040 acctccttca actacggcgc cgtgaaggcc gacaccatca ctgagctttt gatccgcatg 2100 aagcgcgcgg agacatattg ccctaggcct ttactagccc ttgacaccac tcaggaccgc 2160 cgcaaacagg agatcattgc acctgagaag caggttttga actttgacct actcaagttg 2220 gcaggagacg ttgagtccaa ccccgggcc 2249 <210> 368 <211> 2250 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(2250) <220> <221> misc_feature <222> (1)..(2250) <223> P1_Asia1_Shamir_wildtype <400> 368 gga gcc ggt caa tcc agt ccg gca acc ggg tca cag aac caa tct ggc 48 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 aac act gga agc atc att aac aac tac tac atg caa cag tac cag aat 96 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 tcc atg gac aca cag ctt ggt gac aac gct att agc gga ggt tcc aac 144 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 gaa ggt tcc acg gat acc act tcc aca cac aca aac aac acc caa aac 192 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Asn Asn Thr Gln Asn 50 55 60 aac gac tgg ttc tcg cgc ctg gct agc tct gca ttc agt ggt ctc ttt 240 Asn Asp Trp Phe Ser Arg Leu Ala Ser Ser Ala Phe Ser Gly Leu Phe 65 70 75 80 ggt gca ctt ttg gct gac aag aag aca gaa gag aca act ctg ctt gaa 288 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 gac cgc att ctc acc acc agg aac ggc cac aca aca tcg acg aca cag 336 Asp Arg Ile Leu Thr Thr Arg Asn Gly His Thr Thr Ser Thr Thr Gln 100 105 110 tcg agc gtt ggc gta aca tac ggt tac gct gtg gcc gag gac gcg gtg 384 Ser Ser Val Gly Val Thr Tyr Gly Tyr Ala Val Ala Glu Asp Ala Val 115 120 125 tct gga ccc aat acc tcg ggt cta gag act cgt gtt caa cag gca gaa 432 Ser Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Gln Gln Ala Glu 130 135 140 cgg ttt ttc aag aaa cac ctg ttt gac tgg aca ccg aac ttg gca ttt 480 Arg Phe Phe Lys Lys His Leu Phe Asp Trp Thr Pro Asn Leu Ala Phe 145 150 155 160 gga cac tgt tac tac ctg gaa ctt ccc act gaa cac aaa ggc gtg tac 528 Gly His Cys Tyr Tyr Leu Glu Leu Pro Thr Glu His Lys Gly Val Tyr 165 170 175 ggc agt ctc atg ggc tcg tac gcc tac atg aga aat gga tgg gac ata 576 Gly Ser Leu Met Gly Ser Tyr Ala Tyr Met Arg Asn Gly Trp Asp Ile 180 185 190 gag gtg act gct gtt gga aac caa ttc aac ggt ggt tgt ctc ctt gtc 624 Glu Val Thr Ala Val Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu Val 195 200 205 gcg ctc gtg cca gag ctg aag gaa ctc gac acg cga cag aag tac cag 672 Ala Leu Val Pro Glu Leu Lys Glu Leu Asp Thr Arg Gln Lys Tyr Gln 210 215 220 ctg acc ctc ttt ccc cac cag ttc atc aac cca cgc acc aac atg acg 720 Leu Thr Leu Phe Pro His Gln Phe Ile Asn Pro Arg Thr Asn Met Thr 225 230 235 240 gcc cac atc aac gtg ccg tac gtg ggt atc aac agg tac gac cag tac 768 Ala His Ile Asn Val Pro Tyr Val Gly Ile Asn Arg Tyr Asp Gln Tyr 245 250 255 gcc ctc cac aag ccg tgg acg ctt gtt gtg atg gtg gta gcc cca ctc 816 Ala Leu His Lys Pro Trp Thr Leu Val Val Met Val Val Ala Pro Leu 260 265 270 acc gtc aaa act ggt ggt tct gaa cag atc aag gtt tac atg aat gca 864 Thr Val Lys Thr Gly Gly Ser Glu Gln Ile Lys Val Tyr Met Asn Ala 275 280 285 gcg cca acc tac gtg cat gtg gcg gga gag ctg ccc tcg aaa gag gga 912 Ala Pro Thr Tyr Val His Val Ala Gly Glu Leu Pro Ser Lys Glu Gly 290 295 300 ata gtt ccc gtc gcg tgt gcg gac ggt tac ggc aac atg gtg acc acg 960 Ile Val Pro Val Ala Cys Ala Asp Gly Tyr Gly Asn Met Val Thr Thr 305 310 315 320 gac ccg aag acg gcc gat cca gtt tac ggg aaa gtg ttc aac ccc ccc 1008 Asp Pro Lys Thr Ala Asp Pro Val Tyr Gly Lys Val Phe Asn Pro Pro 325 330 335 agg aca aac ctc cct ggg cgc ttc acg aac ttc ctt gat gtt gcg gag 1056 Arg Thr Asn Leu Pro Gly Arg Phe Thr Asn Phe Leu Asp Val Ala Glu 340 345 350 gca tgt cca act ttc ctc cgc ttt gga gaa gta cca ttt gtg aag acg 1104 Ala Cys Pro Thr Phe Leu Arg Phe Gly Glu Val Pro Phe Val Lys Thr 355 360 365 gtg aac tct ggt gac cgc ttg ctg gcc aag ttc gac gtg tcc ctc gct 1152 Val Asn Ser Gly Asp Arg Leu Leu Ala Lys Phe Asp Val Ser Leu Ala 370 375 380 gca ggg cac atg tcc aac acc tac ttg gct ggc ctg gcg cag tac tac 1200 Ala Gly His Met Ser Asn Thr Tyr Leu Ala Gly Leu Ala Gln Tyr Tyr 385 390 395 400 aca cag tac agc ggc acc atg aac gtc cac ttc atg ttc acc ggg ccc 1248 Thr Gln Tyr Ser Gly Thr Met Asn Val His Phe Met Phe Thr Gly Pro 405 410 415 acg gat gct aaa gcc cga tac atg gtg gct tat gtc ccc cct ggc atg 1296 Thr Asp Ala Lys Ala Arg Tyr Met Val Ala Tyr Val Pro Pro Gly Met 420 425 430 aca ccg ccc acg gac cct gag cac gcc gca cac tgc att cac tct gag 1344 Thr Pro Pro Thr Asp Pro Glu His Ala Ala His Cys Ile His Ser Glu 435 440 445 tgg gat act ggt ctt aac tct aag ttt acc ttt tcc ata cct tac ctc 1392 Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro Tyr Leu 450 455 460 tct gct gct gac tat gcc tac act gct tct gac gtg gcg gag acc acg 1440 Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Asp Val Ala Glu Thr Thr 465 470 475 480 agt gtg cag gga tgg gtg tgt atc tat cag atc acc cac ggc aag gct 1488 Ser Val Gln Gly Trp Val Cys Ile Tyr Gln Ile Thr His Gly Lys Ala 485 490 495 gag gga gac gca ctg gtc gtt tct gtc agc gcc ggc aaa gac ttt gag 1536 Glu Gly Asp Ala Leu Val Val Ser Val Ser Ala Gly Lys Asp Phe Glu 500 505 510 ttt cgc ttg cct gtt gac gca cgc cag caa acc acc acc act ggc gaa 1584 Phe Arg Leu Pro Val Asp Ala Arg Gln Gln Thr Thr Thr Thr Gly Glu 515 520 525 tca gca gat cca gtc aca acc acg gtt gag aac tat gga gga gag act 1632 Ser Ala Asp Pro Val Thr Thr Thr Val Glu Asn Tyr Gly Gly Glu Thr 530 535 540 cag aca gcc aga cgg ctt cac act gac gtc gcc ttc att ctt gac agg 1680 Gln Thr Ala Arg Arg Leu His Thr Asp Val Ala Phe Ile Leu Asp Arg 545 550 555 560 ttt gtg aaa ctc act gct ccc aag aac atc caa acc ctc gat ctc atg 1728 Phe Val Lys Leu Thr Ala Pro Lys Asn Ile Gln Thr Leu Asp Leu Met 565 570 575 cag atc ccc tca cac acg ctg gtt gga gca cta ctt cgt tct gcg acg 1776 Gln Ile Pro Ser His Thr Leu Val Gly Ala Leu Leu Arg Ser Ala Thr 580 585 590 tac tac ttc tca gac ctg gag gtc gcg ctt gtc cac aca ggc ccg gtc 1824 Tyr Tyr Phe Ser Asp Leu Glu Val Ala Leu Val His Thr Gly Pro Val 595 600 605 acc tgg gtg ccc aac ggc gcg ccc aag gat gct cta aac aac cag acc 1872 Thr Trp Val Pro Asn Gly Ala Pro Lys Asp Ala Leu Asn Asn Gln Thr 610 615 620 aac cca act gcc tat cag aag caa ccc atc acc cgc ctg gca ctc ccc 1920 Asn Pro Thr Ala Tyr Gln Lys Gln Pro Ile Thr Arg Leu Ala Leu Pro 625 630 635 640 tac acc gcc ccc cat cgt gtg ctg gca aca gtg tac aac ggg aag acg 1968 Tyr Thr Ala Pro His Arg Val Leu Ala Thr Val Tyr Asn Gly Lys Thr 645 650 655 gcg tac ggg gaa acg acc tca agg cgc ggc gac atg gcg gcc ctc gca 2016 Ala Tyr Gly Glu Thr Thr Ser Arg Arg Gly Asp Met Ala Ala Leu Ala 660 665 670 caa agg ttg agc gct cgg ctg ccc acc tcc ttc aac tac ggc gcc gtg 2064 Gln Arg Leu Ser Ala Arg Leu Pro Thr Ser Phe Asn Tyr Gly Ala Val 675 680 685 aag gcc gac acc atc act gag ctt ttg atc cgc atg aag cgc gcg gag 2112 Lys Ala Asp Thr Ile Thr Glu Leu Leu Ile Arg Met Lys Arg Ala Glu 690 695 700 aca tat tgc cct agg cct tta cta gcc ctt gac acc act cag gac cgc 2160 Thr Tyr Cys Pro Arg Pro Leu Leu Ala Leu Asp Thr Thr Gln Asp Arg 705 710 715 720 cgc aaa cag gag atc att gca cct gag aag cag gtt ttg aac ttt gac 2208 Arg Lys Gln Glu Ile Ile Ala Pro Glu Lys Gln Val Leu Asn Phe Asp 725 730 735 cta ctc aag ttg gca gga gac gtt gag tcc aac cct ggg ccc 2250 Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 740 745 750 <210> 369 <211> 750 <212> PRT <213> Foot-and-mouth disease virus <400> 369 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Asn Asn Thr Gln Asn 50 55 60 Asn Asp Trp Phe Ser Arg Leu Ala Ser Ser Ala Phe Ser Gly Leu Phe 65 70 75 80 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 Asp Arg Ile Leu Thr Thr Arg Asn Gly His Thr Thr Ser Thr Thr Gln 100 105 110 Ser Ser Val Gly Val Thr Tyr Gly Tyr Ala Val Ala Glu Asp Ala Val 115 120 125 Ser Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Gln Gln Ala Glu 130 135 140 Arg Phe Phe Lys Lys His Leu Phe Asp Trp Thr Pro Asn Leu Ala Phe 145 150 155 160 Gly His Cys Tyr Tyr Leu Glu Leu Pro Thr Glu His Lys Gly Val Tyr 165 170 175 Gly Ser Leu Met Gly Ser Tyr Ala Tyr Met Arg Asn Gly Trp Asp Ile 180 185 190 Glu Val Thr Ala Val Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu Val 195 200 205 Ala Leu Val Pro Glu Leu Lys Glu Leu Asp Thr Arg Gln Lys Tyr Gln 210 215 220 Leu Thr Leu Phe Pro His Gln Phe Ile Asn Pro Arg Thr Asn Met Thr 225 230 235 240 Ala His Ile Asn Val Pro Tyr Val Gly Ile Asn Arg Tyr Asp Gln Tyr 245 250 255 Ala Leu His Lys Pro Trp Thr Leu Val Val Met Val Val Ala Pro Leu 260 265 270 Thr Val Lys Thr Gly Gly Ser Glu Gln Ile Lys Val Tyr Met Asn Ala 275 280 285 Ala Pro Thr Tyr Val His Val Ala Gly Glu Leu Pro Ser Lys Glu Gly 290 295 300 Ile Val Pro Val Ala Cys Ala Asp Gly Tyr Gly Asn Met Val Thr Thr 305 310 315 320 Asp Pro Lys Thr Ala Asp Pro Val Tyr Gly Lys Val Phe Asn Pro Pro 325 330 335 Arg Thr Asn Leu Pro Gly Arg Phe Thr Asn Phe Leu Asp Val Ala Glu 340 345 350 Ala Cys Pro Thr Phe Leu Arg Phe Gly Glu Val Pro Phe Val Lys Thr 355 360 365 Val Asn Ser Gly Asp Arg Leu Leu Ala Lys Phe Asp Val Ser Leu Ala 370 375 380 Ala Gly His Met Ser Asn Thr Tyr Leu Ala Gly Leu Ala Gln Tyr Tyr 385 390 395 400 Thr Gln Tyr Ser Gly Thr Met Asn Val His Phe Met Phe Thr Gly Pro 405 410 415 Thr Asp Ala Lys Ala Arg Tyr Met Val Ala Tyr Val Pro Pro Gly Met 420 425 430 Thr Pro Pro Thr Asp Pro Glu His Ala Ala His Cys Ile His Ser Glu 435 440 445 Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro Tyr Leu 450 455 460 Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Asp Val Ala Glu Thr Thr 465 470 475 480 Ser Val Gln Gly Trp Val Cys Ile Tyr Gln Ile Thr His Gly Lys Ala 485 490 495 Glu Gly Asp Ala Leu Val Val Ser Val Ser Ala Gly Lys Asp Phe Glu 500 505 510 Phe Arg Leu Pro Val Asp Ala Arg Gln Gln Thr Thr Thr Thr Gly Glu 515 520 525 Ser Ala Asp Pro Val Thr Thr Thr Val Glu Asn Tyr Gly Gly Glu Thr 530 535 540 Gln Thr Ala Arg Arg Leu His Thr Asp Val Ala Phe Ile Leu Asp Arg 545 550 555 560 Phe Val Lys Leu Thr Ala Pro Lys Asn Ile Gln Thr Leu Asp Leu Met 565 570 575 Gln Ile Pro Ser His Thr Leu Val Gly Ala Leu Leu Arg Ser Ala Thr 580 585 590 Tyr Tyr Phe Ser Asp Leu Glu Val Ala Leu Val His Thr Gly Pro Val 595 600 605 Thr Trp Val Pro Asn Gly Ala Pro Lys Asp Ala Leu Asn Asn Gln Thr 610 615 620 Asn Pro Thr Ala Tyr Gln Lys Gln Pro Ile Thr Arg Leu Ala Leu Pro 625 630 635 640 Tyr Thr Ala Pro His Arg Val Leu Ala Thr Val Tyr Asn Gly Lys Thr 645 650 655 Ala Tyr Gly Glu Thr Thr Ser Arg Arg Gly Asp Met Ala Ala Leu Ala 660 665 670 Gln Arg Leu Ser Ala Arg Leu Pro Thr Ser Phe Asn Tyr Gly Ala Val 675 680 685 Lys Ala Asp Thr Ile Thr Glu Leu Leu Ile Arg Met Lys Arg Ala Glu 690 695 700 Thr Tyr Cys Pro Arg Pro Leu Leu Ala Leu Asp Thr Thr Gln Asp Arg 705 710 715 720 Arg Lys Gln Glu Ile Ile Ala Pro Glu Lys Gln Val Leu Asn Phe Asp 725 730 735 Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 740 745 750 <210> 370 <211> 2247 <212> DNA <213> Artificial Sequence <220> <223> P1_C3_cut_sites_removed <400> 370 ggagccggac aatccagccc ggcgactggc tcgcagaacc aatctggcaa cactggtagc 60 ataatcaaca actactacat gcaacagtac caaaattcaa tggacacaca actgggtgac 120 aatgctatta gtggtggctc caacgagggc tccacagata caacttccac ccacacaacc 180 aacactcaaa acaacgactg gttttccaaa ctcgccagtt ctgcctttag cggtcttttc 240 ggtgctcttc ttgccgacaa gaagaccgag gaaaccacac tacttgaaga ccgcatcctc 300 accacccgca acggccacac gacttcgaca actcagtcga gcgttggggt cacatacggg 360 tacgcaacaa ctgaggatag cacgtcaggg cctaacacat ccggccttga gacacgtgtt 420 caccaggcag aacggttttt caagatgaca ctctttgaat gggttccctc ccagagtttt 480 ggacacatgc acaaggtcgt tctgccctca gaaccgaaag gtgtctatgg gggtctcgtc 540 aagtcttatg cgtacatgcg caatggctgg gacgttgagg tgactgctgt tggaaaccag 600 ttcaacggcg gttgtctcct ggtggcgctc gttcctgaaa tgggtgacat cagtgacaga 660 gagaagtacc aactgactct ctacccccac caattcatca acccacgcac taacatgacg 720 gcacacatca ccgtgcctta cgtgggtgtc aacagatacg accaatacaa ccaacacaag 780 ccctggactc ttgtcgtcat ggtcgttgct ccacttactg tgaacacatc aggtgcccag 840 cagatcaagg tgtatgccaa catagcccca accaacgttc acgttgctgg tgaacttccc 900 tccaaggagg ggatcttccc cgttgcgtgt gccgacggct atggcaacat ggtgacaact 960 gacccgaaga cggctgaccc tgcctacggg aaagtctata atccacccag gaccgccctg 1020 ccgggccggt tcacaaacta cctggatgtt gctgaggctt gccccactct cctgacgttc 1080 gagaacgtgc cttacgtttc aacacggact gatggacaaa ggctgttggc caagttcgac 1140 gtgtcattgg cagcgaaaca catgtcaaac acttacttgg ctggcttggc ccagtactac 1200 acacagtacg ctgggacaat caacctgcac ttcatgttca ctgggccaac cgacgcgaaa 1260 gctcggtaca tggtggcata cgtgccccct ggcatggaag caccagacaa cccagaggag 1320 gctgcccact gcatacacgc agagtgggac actggtttga actctaagtt cacattttca 1380 atcccgtaca tctcggccgc tgactacgca tacaccgcgt ccagcgaggc tgaaacaaca 1440 agcgtacagg gatgggtttg tgtgtaccag atcactcacg gcaaggcaga cgctgacgcg 1500 ctcgtcgtct ccgcttcggc ggggaaagac tttgaactcc ggctacctgt ggacgctaga 1560 cagcaaacta cgaccactgg cgaatctgcc gaccccgtca ccactaccgt tgagaactac 1620 ggaggagaaa cacaaactca acgtcgccac cacactgacg ttgccttcgt tcttgatcgg 1680 tttgtgaagg tccaggtgtc gggcaaccaa cacacactgg acgttatgca ggtacacaag 1740 gacagtattg tgggtgcact cctacgcgca gccacatact acttctctga cttggaaata 1800 gcagtgactc acactgggaa gctcacatgg gtgcccaacg gcgccccagt ttctgcactt 1860 gacaacacaa ccaaccccac tgcctaccac aaggggccgc tgactcggct ggctctccca 1920 tacaccgcac cacaccgcgt gctggccacg gcgtacactg gtacaacggc ctacactaca 1980 ggtgtacgca ggggagacct agcccacttg gcggcggcgc acgctcggca cctgccaacg 2040 tcgttcaact ttggtgcagt taaagcagag acaatcacag agctgcttgt gcgcatgaag 2100 cgtgctgaac tctactgccc caggccggtc cttccggtcc aaccagcggg cgataggcac 2160 aaacaaccgc tcattgcgcc agcgaaacaa ctgctgaact tcgaccttct caagttggcg 2220 ggagatgtcg agtccaaccc tgggccc 2247 <210> 371 <211> 2247 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(2247) <220> <221> misc_feature <222> (1)..(2247) <223> P1_C3_wildtype <400> 371 gga gcc gga caa tcc agc ccg gcg act ggc tcg cag aac caa tct ggc 48 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 aac act ggt agc ata atc aac aac tac tac atg caa cag tac caa aat 96 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 tcc atg gac aca cag ctg ggt gac aat gct att agt ggt ggc tcc aac 144 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 gag ggc tcc aca gat aca act tcc acc cac aca acc aac act caa aac 192 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Thr Asn Thr Gln Asn 50 55 60 aac gac tgg ttt tcc aaa ctc gcc agt tct gcc ttt agc ggt ctt ttc 240 Asn Asp Trp Phe Ser Lys Leu Ala Ser Ser Ala Phe Ser Gly Leu Phe 65 70 75 80 ggt gct ctt ctt gcc gac aag aag acc gag gaa acc aca cta ctt gaa 288 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 gac cgc atc ctc acc acc cgc aac ggc cac acg acg tcg aca act cag 336 Asp Arg Ile Leu Thr Thr Arg Asn Gly His Thr Thr Ser Thr Thr Gln 100 105 110 tcg agc gtt ggg gtc aca tac ggg tac gca aca act gag gat agc acg 384 Ser Ser Val Gly Val Thr Tyr Gly Tyr Ala Thr Thr Glu Asp Ser Thr 115 120 125 tca ggg ccc aac aca tcc ggc ctt gag aca cgt gtt cac cag gca gaa 432 Ser Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val His Gln Ala Glu 130 135 140 cgg ttt ttc aag atg aca ctc ttt gaa tgg gtt ccc tcc cag agt ttt 480 Arg Phe Phe Lys Met Thr Leu Phe Glu Trp Val Pro Ser Gln Ser Phe 145 150 155 160 gga cac atg cac aag gtc gtt ctg ccc tca gaa ccg aaa ggt gtc tat 528 Gly His Met His Lys Val Val Leu Pro Ser Glu Pro Lys Gly Val Tyr 165 170 175 ggg ggt ctc gtc aag tca tac gcg tac atg cgc aat ggc tgg gac gtt 576 Gly Gly Leu Val Lys Ser Tyr Ala Tyr Met Arg Asn Gly Trp Asp Val 180 185 190 gag gtg act gct gtt gga aac cag ttc aac ggc ggt tgt ctc ctg gtg 624 Glu Val Thr Ala Val Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu Val 195 200 205 gcg ctc gtt cct gaa atg ggt gac atc agt gac aga gag aag tac caa 672 Ala Leu Val Pro Glu Met Gly Asp Ile Ser Asp Arg Glu Lys Tyr Gln 210 215 220 ctg act ctc tac ccc cac caa ttc atc aac cca cgc act aac atg acg 720 Leu Thr Leu Tyr Pro His Gln Phe Ile Asn Pro Arg Thr Asn Met Thr 225 230 235 240 gca cac atc acc gtg cct tac gtg ggt gtc aac aga tac gac caa tac 768 Ala His Ile Thr Val Pro Tyr Val Gly Val Asn Arg Tyr Asp Gln Tyr 245 250 255 aac caa cac aag ccc tgg act ctt gtc gtc atg gtc gtt gct cca ctt 816 Asn Gln His Lys Pro Trp Thr Leu Val Val Met Val Val Ala Pro Leu 260 265 270 act gtg aac aca tca ggt gcc cag cag atc aag gtg tat gcc aac ata 864 Thr Val Asn Thr Ser Gly Ala Gln Gln Ile Lys Val Tyr Ala Asn Ile 275 280 285 gcc cca acc aac gtt cac gtt gct ggt gaa ctt ccc tcc aag gag ggg 912 Ala Pro Thr Asn Val His Val Ala Gly Glu Leu Pro Ser Lys Glu Gly 290 295 300 atc ttc ccc gtt gcg tgt gcc gac ggc tat ggc aac atg gtg aca act 960 Ile Phe Pro Val Ala Cys Ala Asp Gly Tyr Gly Asn Met Val Thr Thr 305 310 315 320 gac ccg aag aca gct gac cct gcc tac ggg aaa gtc tac aat cca ccc 1008 Asp Pro Lys Thr Ala Asp Pro Ala Tyr Gly Lys Val Tyr Asn Pro Pro 325 330 335 agg acc gcc ctg ccg ggc cgg ttc aca aac tac ctg gat gtt gct gag 1056 Arg Thr Ala Leu Pro Gly Arg Phe Thr Asn Tyr Leu Asp Val Ala Glu 340 345 350 gct tgc ccc act ctc ctg acg ttc gag aac gtg cct tac gtt tca aca 1104 Ala Cys Pro Thr Leu Leu Thr Phe Glu Asn Val Pro Tyr Val Ser Thr 355 360 365 cgg act gat gga caa agg ctg ttg gcc aag ttc gac gtg tca ttg gca 1152 Arg Thr Asp Gly Gln Arg Leu Leu Ala Lys Phe Asp Val Ser Leu Ala 370 375 380 gcg aaa cac atg tca aac act tac ttg gct ggc ttg gcc cag tac tac 1200 Ala Lys His Met Ser Asn Thr Tyr Leu Ala Gly Leu Ala Gln Tyr Tyr 385 390 395 400 aca cag tac gct ggg aca atc aac ctg cac ttc atg ttc act ggg cca 1248 Thr Gln Tyr Ala Gly Thr Ile Asn Leu His Phe Met Phe Thr Gly Pro 405 410 415 acc gac gcg aaa gct cgg tac atg gtg gca tac gtg ccc cct ggc atg 1296 Thr Asp Ala Lys Ala Arg Tyr Met Val Ala Tyr Val Pro Pro Gly Met 420 425 430 gaa gca cca gac aac cca gag gag gct gcc cac tgc ata cac gca gag 1344 Glu Ala Pro Asp Asn Pro Glu Glu Ala Ala His Cys Ile His Ala Glu 435 440 445 tgg gac act ggt ttg aac tct aag ttc aca ttt tca atc ccg tac atc 1392 Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro Tyr Ile 450 455 460 tcg gcc gct gac tac gca tac acc gcg tcc agc gag gct gaa aca aca 1440 Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Ser Glu Ala Glu Thr Thr 465 470 475 480 agc gta cag gga tgg gtt tgt gtg tac cag atc act cac ggc aag gca 1488 Ser Val Gln Gly Trp Val Cys Val Tyr Gln Ile Thr His Gly Lys Ala 485 490 495 gac gct gac gcg ctc gtc gtc tcc gct tcg gcg ggg aaa gac ttt gag 1536 Asp Ala Asp Ala Leu Val Val Ser Ala Ser Ala Gly Lys Asp Phe Glu 500 505 510 ctc cgg cta cct gtg gac gct aga cag caa act acg acc act ggc gaa 1584 Leu Arg Leu Pro Val Asp Ala Arg Gln Gln Thr Thr Thr Thr Gly Glu 515 520 525 tct gcc gac ccc gtc acc act acc gtt gag aac tac gga gga gaa aca 1632 Ser Ala Asp Pro Val Thr Thr Thr Val Glu Asn Tyr Gly Gly Glu Thr 530 535 540 caa act caa cgt cgc cac cac act gac gtt gcc ttc gtt ctt gac cgg 1680 Gln Thr Gln Arg Arg His His Thr Asp Val Ala Phe Val Leu Asp Arg 545 550 555 560 ttt gtg aag gtc cag gtg tcg ggc aac caa cac aca ctg gac gtt atg 1728 Phe Val Lys Val Gln Val Ser Gly Asn Gln His Thr Leu Asp Val Met 565 570 575 cag gta cac aag gac agt att gtg ggt gca ctc cta cgc gca gcc aca 1776 Gln Val His Lys Asp Ser Ile Val Gly Ala Leu Leu Arg Ala Ala Thr 580 585 590 tac tac ttc tct gac ttg gaa ata gca gtg act cac act ggg aag ctc 1824 Tyr Tyr Phe Ser Asp Leu Glu Ile Ala Val Thr His Thr Gly Lys Leu 595 600 605 aca tgg gtg ccc aac ggc gcc cca gtt tct gca ctt gac aac aca acc 1872 Thr Trp Val Pro Asn Gly Ala Pro Val Ser Ala Leu Asp Asn Thr Thr 610 615 620 aac ccc act gcc tac cac aag ggg ccg ctg act cgg ctg gct ctc cca 1920 Asn Pro Thr Ala Tyr His Lys Gly Pro Leu Thr Arg Leu Ala Leu Pro 625 630 635 640 tac acc gca cca cac cgc gtg ctg gcc acg gcg tac acc ggt aca acg 1968 Tyr Thr Ala Pro His Arg Val Leu Ala Thr Ala Tyr Thr Gly Thr Thr 645 650 655 gcc tac act acc ggt gta cgc agg gga gac cta gcc cac ttg gcg gcg 2016 Ala Tyr Thr Thr Gly Val Arg Arg Gly Asp Leu Ala His Leu Ala Ala 660 665 670 gcg cac gct cgg cac ctg ccg acg tcg ttc aac ttt ggt gca gtt aaa 2064 Ala His Ala Arg His Leu Pro Thr Ser Phe Asn Phe Gly Ala Val Lys 675 680 685 gca gag aca atc aca gag ctg ctt gtg cgc atg aag cgt gct gaa ctc 2112 Ala Glu Thr Ile Thr Glu Leu Leu Val Arg Met Lys Arg Ala Glu Leu 690 695 700 tac tgc ccc aga ccg gtc ctt ccg gtc caa cca gcg ggc gat agg cac 2160 Tyr Cys Pro Arg Pro Val Leu Pro Val Gln Pro Ala Gly Asp Arg His 705 710 715 720 aaa caa ccg ctc att gcg cca gcg aaa cag ctg ctg aac ttc gac ctt 2208 Lys Gln Pro Leu Ile Ala Pro Ala Lys Gln Leu Leu Asn Phe Asp Leu 725 730 735 ctc aag ttg gcg gga gac gtc gag tcc aac cct ggg ccc 2247 Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 740 745 <210> 372 <211> 749 <212> PRT <213> Foot-and-mouth disease virus <400> 372 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Thr Asn Thr Gln Asn 50 55 60 Asn Asp Trp Phe Ser Lys Leu Ala Ser Ser Ala Phe Ser Gly Leu Phe 65 70 75 80 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 Asp Arg Ile Leu Thr Thr Arg Asn Gly His Thr Thr Ser Thr Thr Gln 100 105 110 Ser Ser Val Gly Val Thr Tyr Gly Tyr Ala Thr Thr Glu Asp Ser Thr 115 120 125 Ser Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val His Gln Ala Glu 130 135 140 Arg Phe Phe Lys Met Thr Leu Phe Glu Trp Val Pro Ser Gln Ser Phe 145 150 155 160 Gly His Met His Lys Val Val Leu Pro Ser Glu Pro Lys Gly Val Tyr 165 170 175 Gly Gly Leu Val Lys Ser Tyr Ala Tyr Met Arg Asn Gly Trp Asp Val 180 185 190 Glu Val Thr Ala Val Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu Val 195 200 205 Ala Leu Val Pro Glu Met Gly Asp Ile Ser Asp Arg Glu Lys Tyr Gln 210 215 220 Leu Thr Leu Tyr Pro His Gln Phe Ile Asn Pro Arg Thr Asn Met Thr 225 230 235 240 Ala His Ile Thr Val Pro Tyr Val Gly Val Asn Arg Tyr Asp Gln Tyr 245 250 255 Asn Gln His Lys Pro Trp Thr Leu Val Val Met Val Val Ala Pro Leu 260 265 270 Thr Val Asn Thr Ser Gly Ala Gln Gln Ile Lys Val Tyr Ala Asn Ile 275 280 285 Ala Pro Thr Asn Val His Val Ala Gly Glu Leu Pro Ser Lys Glu Gly 290 295 300 Ile Phe Pro Val Ala Cys Ala Asp Gly Tyr Gly Asn Met Val Thr Thr 305 310 315 320 Asp Pro Lys Thr Ala Asp Pro Ala Tyr Gly Lys Val Tyr Asn Pro Pro 325 330 335 Arg Thr Ala Leu Pro Gly Arg Phe Thr Asn Tyr Leu Asp Val Ala Glu 340 345 350 Ala Cys Pro Thr Leu Leu Thr Phe Glu Asn Val Pro Tyr Val Ser Thr 355 360 365 Arg Thr Asp Gly Gln Arg Leu Leu Ala Lys Phe Asp Val Ser Leu Ala 370 375 380 Ala Lys His Met Ser Asn Thr Tyr Leu Ala Gly Leu Ala Gln Tyr Tyr 385 390 395 400 Thr Gln Tyr Ala Gly Thr Ile Asn Leu His Phe Met Phe Thr Gly Pro 405 410 415 Thr Asp Ala Lys Ala Arg Tyr Met Val Ala Tyr Val Pro Pro Gly Met 420 425 430 Glu Ala Pro Asp Asn Pro Glu Glu Ala Ala His Cys Ile His Ala Glu 435 440 445 Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro Tyr Ile 450 455 460 Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Ser Glu Ala Glu Thr Thr 465 470 475 480 Ser Val Gln Gly Trp Val Cys Val Tyr Gln Ile Thr His Gly Lys Ala 485 490 495 Asp Ala Asp Ala Leu Val Val Ser Ala Ser Ala Gly Lys Asp Phe Glu 500 505 510 Leu Arg Leu Pro Val Asp Ala Arg Gln Gln Thr Thr Thr Thr Gly Glu 515 520 525 Ser Ala Asp Pro Val Thr Thr Thr Val Glu Asn Tyr Gly Gly Glu Thr 530 535 540 Gln Thr Gln Arg Arg His His Thr Asp Val Ala Phe Val Leu Asp Arg 545 550 555 560 Phe Val Lys Val Gln Val Ser Gly Asn Gln His Thr Leu Asp Val Met 565 570 575 Gln Val His Lys Asp Ser Ile Val Gly Ala Leu Leu Arg Ala Ala Thr 580 585 590 Tyr Tyr Phe Ser Asp Leu Glu Ile Ala Val Thr His Thr Gly Lys Leu 595 600 605 Thr Trp Val Pro Asn Gly Ala Pro Val Ser Ala Leu Asp Asn Thr Thr 610 615 620 Asn Pro Thr Ala Tyr His Lys Gly Pro Leu Thr Arg Leu Ala Leu Pro 625 630 635 640 Tyr Thr Ala Pro His Arg Val Leu Ala Thr Ala Tyr Thr Gly Thr Thr 645 650 655 Ala Tyr Thr Thr Gly Val Arg Arg Gly Asp Leu Ala His Leu Ala Ala 660 665 670 Ala His Ala Arg His Leu Pro Thr Ser Phe Asn Phe Gly Ala Val Lys 675 680 685 Ala Glu Thr Ile Thr Glu Leu Leu Val Arg Met Lys Arg Ala Glu Leu 690 695 700 Tyr Cys Pro Arg Pro Val Leu Pro Val Gln Pro Ala Gly Asp Arg His 705 710 715 720 Lys Gln Pro Leu Ile Ala Pro Ala Lys Gln Leu Leu Asn Phe Asp Leu 725 730 735 Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 740 745 <210> 373 <211> 2259 <212> DNA <213> Artificial Sequence <220> <223> P1_O1_Manissa_cut_sites_removed <400> 373 ggagccgggc aatccagccc ggcaaccggg tcacagaacc aatcaggcaa cactgggagc 60 atcatcaaca attactacat gcagcagtac caaaactcta tggacacaca acttggtgac 120 aacgctacaa gcggaggctc aaacgagggg tccacggaca caacctccac ccacacaacc 180 aacactcaga acaacgactg gttctcgaag ctggccagtt ccgctttcag cggtcttttc 240 ggcgctcttc tcgccgacaa gaaaaccgag gagaccactc ttcttgagga ccgcatcctc 300 actactcgta acggacacac cacctcgaca acccagtcga gcgtaggagt cacatacggg 360 tatgcaacgg ctgaggattt cgtgagcggg ccaaacacct ctggtcttga gaccagggtt 420 gcccaggcag agcggttctt taaaacccac ctgttcgact gggtcacaag tgacccgttc 480 ggacggtgcc acctgctaga acttccaact gaccacaaag gtgtctatgg cagcctgacc 540 gactcgtatg cttatatgag gaacggctgg gatgttgaag tcactgctgt gggaaatcag 600 ttcaatggag gatgcctgtt ggtggctatg gtgccagaac tttgctccat acagaagagg 660 gagctgtacc agctcacgct ctttcctcac cagttcatca accctcggac gaacatgaca 720 gcacacatca ctgtgccctt tgttggcgtc aaccgttatg accagtacaa ggtacacaaa 780 ccttggaccc tcgtggttat ggttgtagcc cccctgaccg tcaacagtga aggtgccccg 840 caaatcaagg tgtatgccaa catcgcacct accaacgtac acgtcgcggg tgagttccct 900 tccaaagagg ggatcttccc tgtggcttgc agcgatggtt atggcggtct ggtgacaact 960 gacccgaaaa cggctgaccc cgcttacggg aaagtgttta accccccccg caacatgttg 1020 ccggggcggt tcaccaattt tcttgacgtg gctgaggcgt gccccacgtt tctccacttc 1080 gagggtgacg tgccatacgt gaccacgaag acggattcag acagggtgct cgctcagttc 1140 gacttgtctt tggcagcaaa gcacatgtcc aacaccttcc ttgcaggtct cgcccagtac 1200 tacacacagt acagcggcac catcaacctg cacttcatgt tcacagggcc tactgacgcg 1260 aaggcgcgtt acatgattgc gtatgctcct cctggcatgg aaccacctaa aacgccagag 1320 gcggctgccc actgcatcca tgctgaatgg gacacagggt tgaactcaaa attcacattt 1380 tcaatccctt acctttcggc ggctgattac gcttacacag cgtctgacac tgctgagacc 1440 acaaatgtac agggatgggt ttgcctgttt caaataacac acgggaaagc tgacggcgac 1500 gcactggtcg ttttggccag cgccggaaag gactttgagc tgcgcctgcc ggtggatgct 1560 cgcacacaga ctacctcagc gggcgagtca gcagaccccg tgaccgccac cgttgagaat 1620 tacggtggcg agacacaggt ccagaggcgc caacacacgg acgtgtcatt tatattagac 1680 agatttgtga aagtgacacc aaaagaccaa attaatgtat tggacctgat gcaaacccct 1740 gctcacactt tggtgggagc actccttcgt actgccactt actatttcgc tgacttagag 1800 gtggcagtga agcacgaggg aaacctcacc tgggtgccga acggggcgcc tgaagcggcg 1860 ttggacaaca ccaccaaccc aacagcttac cacaaggcac cactcacccg acttgcactg 1920 ccttacacgg cgccacaccg cgtgttggct actgtttaca acgggaacag caagtatggt 1980 gacggcacgg tggccaatgt gagaggtgat ctgcaagtgt tggcccagaa ggcggcgaga 2040 gcgctgccta cctccttcaa ctacggtgcc attaaagcta ctcgggtgac tgaactgctt 2100 taccgcatga agagggctga gacatactgt ccccggcctc ttttggccat tcacccggac 2160 caggctagac acaagcagaa gattgtggct ccggtgaaac agcttctaaa ttttgacctg 2220 ctcaaattgg cgggagatgt ggagtccaac cctgggccc 2259 <210> 374 <211> 2259 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(2259) <220> <221> misc_feature <222> (1)..(2259) <223> P1_01_Marissa_wild_type <400> 374 gga gcc ggg caa tcc agc ccg gca acc ggg tca cag aac caa tca ggc 48 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 aac act ggg agc atc atc aac aat tac tac atg cag cag tac caa aac 96 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 tcc atg gac aca caa ctt ggt gac aac gct aca agc gga ggc tca aac 144 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Thr Ser Gly Gly Ser Asn 35 40 45 gag ggg tcc acg gac aca acc tcc acc cac aca acc aac act cag aac 192 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Thr Asn Thr Gln Asn 50 55 60 aac gac tgg ttc tcg aag ctg gcc agt tcc gct ttc agc ggt ctt ttc 240 Asn Asp Trp Phe Ser Lys Leu Ala Ser Ser Ala Phe Ser Gly Leu Phe 65 70 75 80 ggc gct ctt ctc gcc gac aag aaa acc gag gag acc act ctt ctc gag 288 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 gac cgc atc ctc act act cgt aac gga cac acc acc tcg aca acc cag 336 Asp Arg Ile Leu Thr Thr Arg Asn Gly His Thr Thr Ser Thr Thr Gln 100 105 110 tcg agc gtt gga gtc acg tac ggg tat gca aca gct gag gat ttc gtg 384 Ser Ser Val Gly Val Thr Tyr Gly Tyr Ala Thr Ala Glu Asp Phe Val 115 120 125 agc ggg cca aac acc tct ggt ctc gag acc agg gtt gcc cag gca gag 432 Ser Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Ala Gln Ala Glu 130 135 140 cgg ttc ttt aaa acc cac ctg ttc gac tgg gtc acc agt gac ccg ttc 480 Arg Phe Phe Lys Thr His Leu Phe Asp Trp Val Thr Ser Asp Pro Phe 145 150 155 160 gga cgg tgc cac ctg ctg gaa ctt cca act gac cac aaa ggt gtc tac 528 Gly Arg Cys His Leu Leu Glu Leu Pro Thr Asp His Lys Gly Val Tyr 165 170 175 ggc agc ctg acc gac tcg tat gct tat atg agg aac ggc tgg gat gtt 576 Gly Ser Leu Thr Asp Ser Tyr Ala Tyr Met Arg Asn Gly Trp Asp Val 180 185 190 gaa gtc act gca gtg gga aac cag ttc aat gga gga tgc ctg ttg gtg 624 Glu Val Thr Ala Val Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu Val 195 200 205 gcc atg gtg cca gaa ctt tgc tcc ata cag aag agg gag ctg tac cag 672 Ala Met Val Pro Glu Leu Cys Ser Ile Gln Lys Arg Glu Leu Tyr Gln 210 215 220 ctc acg ctc ttt cct cac cag ttc atc aac cct cgg acg aac atg aca 720 Leu Thr Leu Phe Pro His Gln Phe Ile Asn Pro Arg Thr Asn Met Thr 225 230 235 240 gca cac atc act gtg ccc ttt gtt ggc gtc aac cgt tat gac cag tac 768 Ala His Ile Thr Val Pro Phe Val Gly Val Asn Arg Tyr Asp Gln Tyr 245 250 255 aag gta cac aaa cct tgg acc ctc gtg gtt atg gtt gta gcc ccc ctg 816 Lys Val His Lys Pro Trp Thr Leu Val Val Met Val Val Ala Pro Leu 260 265 270 acc gtc aac agt gaa ggt gcc ccg caa atc aag gtg tat gcc aac atc 864 Thr Val Asn Ser Glu Gly Ala Pro Gln Ile Lys Val Tyr Ala Asn Ile 275 280 285 gca cct acc aac gta cac gtc gcg ggt gag ttc cct tcc aaa gag ggg 912 Ala Pro Thr Asn Val His Val Ala Gly Glu Phe Pro Ser Lys Glu Gly 290 295 300 atc ttc cct gtg gct tgc agc gat ggt tat ggc ggt ctg gtg acc act 960 Ile Phe Pro Val Ala Cys Ser Asp Gly Tyr Gly Gly Leu Val Thr Thr 305 310 315 320 gac ccg aaa acg gct gac ccc gct tac ggg aaa gtg ttt aac ccc ccc 1008 Asp Pro Lys Thr Ala Asp Pro Ala Tyr Gly Lys Val Phe Asn Pro Pro 325 330 335 cgc aac atg ttg ccg ggg cgg ttc acc aat ttt ctt gac gtg gct gag 1056 Arg Asn Met Leu Pro Gly Arg Phe Thr Asn Phe Leu Asp Val Ala Glu 340 345 350 gcg tgc ccc acg ttt ctc cac ttc gag ggt gac gtg cca tac gtg acc 1104 Ala Cys Pro Thr Phe Leu His Phe Glu Gly Asp Val Pro Tyr Val Thr 355 360 365 acg aag acg gat tca gac agg gtg ctc gct cag ttc gac ttg tct ttg 1152 Thr Lys Thr Asp Ser Asp Arg Val Leu Ala Gln Phe Asp Leu Ser Leu 370 375 380 gca gca aag cac atg tcg aac acc ttc ctt gca ggt ctc gcc cag tac 1200 Ala Ala Lys His Met Ser Asn Thr Phe Leu Ala Gly Leu Ala Gln Tyr 385 390 395 400 tac aca cag tac agc ggc acc atc aac ctg cac ttc atg ttc aca ggg 1248 Tyr Thr Gln Tyr Ser Gly Thr Ile Asn Leu His Phe Met Phe Thr Gly 405 410 415 cct act gac gcg aag gcg cgt tac atg att gcg tat gct cct cct ggc 1296 Pro Thr Asp Ala Lys Ala Arg Tyr Met Ile Ala Tyr Ala Pro Pro Gly 420 425 430 atg gaa cca cct aaa acg cca gag gcg gct gcc cac tgc att cat gct 1344 Met Glu Pro Pro Lys Thr Pro Glu Ala Ala Ala His Cys Ile His Ala 435 440 445 gaa tgg gac aca ggg ttg aac tca aaa ttc aca ttt tca atc cct tac 1392 Glu Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro Tyr 450 455 460 ctt tcg gcg gct gat tac gct tac aca gcg tct gac act gct gag acc 1440 Leu Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Asp Thr Ala Glu Thr 465 470 475 480 aca aat gta cag gga tgg gtt tgc ctg ttt caa ata aca cac ggg aaa 1488 Thr Asn Val Gln Gly Trp Val Cys Leu Phe Gln Ile Thr His Gly Lys 485 490 495 gct gac ggc gac gca ctg gtc gtt ttg gct agc gcc gga aag gac ttt 1536 Ala Asp Gly Asp Ala Leu Val Val Leu Ala Ser Ala Gly Lys Asp Phe 500 505 510 gag ctg cgc ctg ccg gtg gat gct cgc aca cag act acc tcc gcg ggc 1584 Glu Leu Arg Leu Pro Val Asp Ala Arg Thr Gln Thr Thr Ser Ala Gly 515 520 525 gag tca gct gac ccc gtg acc gcc acc gtt gag aat tac ggt ggc gag 1632 Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu Asn Tyr Gly Gly Glu 530 535 540 aca cag gtc cag agg cgc caa cac acg gac gtc tca ttt ata tta gac 1680 Thr Gln Val Gln Arg Arg Gln His Thr Asp Val Ser Phe Ile Leu Asp 545 550 555 560 aga ttt gtg aaa gtg aca cca aaa gac caa att aat gta ttg gac ctg 1728 Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile Asn Val Leu Asp Leu 565 570 575 atg caa acc cct gct cac act ttg gtg gga gca ctc ctt cgt act gcc 1776 Met Gln Thr Pro Ala His Thr Leu Val Gly Ala Leu Leu Arg Thr Ala 580 585 590 act tac tat ttc gct gac tta gag gtg gca gtg aag cac gag gga aac 1824 Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val Lys His Glu Gly Asn 595 600 605 ctc acc tgg gtc ccg aac ggg gcg cct gaa gcg gcg ttg gac aac acc 1872 Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala Ala Leu Asp Asn Thr 610 615 620 acc aac cca aca gct tac cac aag gca cca ctc acc cga ctt gca ctg 1920 Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu Thr Arg Leu Ala Leu 625 630 635 640 cct tac acg gcg cca cac cgc gtg ttg gct act gtt tac aac ggg aac 1968 Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr Val Tyr Asn Gly Asn 645 650 655 agc aag tat ggt gac ggc acg gtg gcc aat gtg aga ggt gac ctg caa 2016 Ser Lys Tyr Gly Asp Gly Thr Val Ala Asn Val Arg Gly Asp Leu Gln 660 665 670 gtg ttg gcc cag aag gcg gcg aga gcg ctg cct acc tcc ttc aac tac 2064 Val Leu Ala Gln Lys Ala Ala Arg Ala Leu Pro Thr Ser Phe Asn Tyr 675 680 685 ggt gcc att aaa gct act cgg gtg act gaa ctg ctt tac cgc atg aag 2112 Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu Leu Tyr Arg Met Lys 690 695 700 agg gct gag aca tac tgt ccc cgg cct ctt ttg gcc att cac ccg gac 2160 Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu Ala Ile His Pro Asp 705 710 715 720 cag gct aga cac aag cag aag att gtg gca ccg gtg aaa cag ctt cta 2208 Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Leu Leu 725 730 735 aat ttt gac ctg ctc aaa ttg gcg gga gat gtg gag tcc aac cct ggg 2256 Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 740 745 750 ccc 2259 Pro <210> 375 <211> 753 <212> PRT <213> Foot-and-mouth disease virus <400> 375 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Thr Ser Gly Gly Ser Asn 35 40 45 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Thr Asn Thr Gln Asn 50 55 60 Asn Asp Trp Phe Ser Lys Leu Ala Ser Ser Ala Phe Ser Gly Leu Phe 65 70 75 80 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 Asp Arg Ile Leu Thr Thr Arg Asn Gly His Thr Thr Ser Thr Thr Gln 100 105 110 Ser Ser Val Gly Val Thr Tyr Gly Tyr Ala Thr Ala Glu Asp Phe Val 115 120 125 Ser Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Ala Gln Ala Glu 130 135 140 Arg Phe Phe Lys Thr His Leu Phe Asp Trp Val Thr Ser Asp Pro Phe 145 150 155 160 Gly Arg Cys His Leu Leu Glu Leu Pro Thr Asp His Lys Gly Val Tyr 165 170 175 Gly Ser Leu Thr Asp Ser Tyr Ala Tyr Met Arg Asn Gly Trp Asp Val 180 185 190 Glu Val Thr Ala Val Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu Val 195 200 205 Ala Met Val Pro Glu Leu Cys Ser Ile Gln Lys Arg Glu Leu Tyr Gln 210 215 220 Leu Thr Leu Phe Pro His Gln Phe Ile Asn Pro Arg Thr Asn Met Thr 225 230 235 240 Ala His Ile Thr Val Pro Phe Val Gly Val Asn Arg Tyr Asp Gln Tyr 245 250 255 Lys Val His Lys Pro Trp Thr Leu Val Val Met Val Val Ala Pro Leu 260 265 270 Thr Val Asn Ser Glu Gly Ala Pro Gln Ile Lys Val Tyr Ala Asn Ile 275 280 285 Ala Pro Thr Asn Val His Val Ala Gly Glu Phe Pro Ser Lys Glu Gly 290 295 300 Ile Phe Pro Val Ala Cys Ser Asp Gly Tyr Gly Gly Leu Val Thr Thr 305 310 315 320 Asp Pro Lys Thr Ala Asp Pro Ala Tyr Gly Lys Val Phe Asn Pro Pro 325 330 335 Arg Asn Met Leu Pro Gly Arg Phe Thr Asn Phe Leu Asp Val Ala Glu 340 345 350 Ala Cys Pro Thr Phe Leu His Phe Glu Gly Asp Val Pro Tyr Val Thr 355 360 365 Thr Lys Thr Asp Ser Asp Arg Val Leu Ala Gln Phe Asp Leu Ser Leu 370 375 380 Ala Ala Lys His Met Ser Asn Thr Phe Leu Ala Gly Leu Ala Gln Tyr 385 390 395 400 Tyr Thr Gln Tyr Ser Gly Thr Ile Asn Leu His Phe Met Phe Thr Gly 405 410 415 Pro Thr Asp Ala Lys Ala Arg Tyr Met Ile Ala Tyr Ala Pro Pro Gly 420 425 430 Met Glu Pro Pro Lys Thr Pro Glu Ala Ala Ala His Cys Ile His Ala 435 440 445 Glu Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro Tyr 450 455 460 Leu Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Asp Thr Ala Glu Thr 465 470 475 480 Thr Asn Val Gln Gly Trp Val Cys Leu Phe Gln Ile Thr His Gly Lys 485 490 495 Ala Asp Gly Asp Ala Leu Val Val Leu Ala Ser Ala Gly Lys Asp Phe 500 505 510 Glu Leu Arg Leu Pro Val Asp Ala Arg Thr Gln Thr Thr Ser Ala Gly 515 520 525 Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu Asn Tyr Gly Gly Glu 530 535 540 Thr Gln Val Gln Arg Arg Gln His Thr Asp Val Ser Phe Ile Leu Asp 545 550 555 560 Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile Asn Val Leu Asp Leu 565 570 575 Met Gln Thr Pro Ala His Thr Leu Val Gly Ala Leu Leu Arg Thr Ala 580 585 590 Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val Lys His Glu Gly Asn 595 600 605 Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala Ala Leu Asp Asn Thr 610 615 620 Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu Thr Arg Leu Ala Leu 625 630 635 640 Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr Val Tyr Asn Gly Asn 645 650 655 Ser Lys Tyr Gly Asp Gly Thr Val Ala Asn Val Arg Gly Asp Leu Gln 660 665 670 Val Leu Ala Gln Lys Ala Ala Arg Ala Leu Pro Thr Ser Phe Asn Tyr 675 680 685 Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu Leu Tyr Arg Met Lys 690 695 700 Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu Ala Ile His Pro Asp 705 710 715 720 Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Leu Leu 725 730 735 Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 740 745 750 Pro <210> 376 <211> 2259 <212> DNA <213> Artificial Sequence <220> <223> P1_O1_pan_Asia_cut_sites_removed <400> 376 ggtgctgggc aatccagccc ggcgactggg tcacagaacc agtcaggcaa cactggaagc 60 atcatcaaca actactacat gcagcagtac cagaactcca tggacacaca acttggtgac 120 aacgctatca gcggaggctc taacgaaggt tctacagaca ccacctccac ccacacaacc 180 aacactcaga acaacgactg gttttcaaaa ctagccagtt ccgctttcag cggtcttttc 240 ggcgctcttc tcgccgacaa gaaaaccgag gagactactc ttcttgagga ccgcatcctc 300 actacccgta acggacacac aacctcgaca acccagtcga gcgttggagt cacttacggg 360 tacgcaacag ccgaggattt tgtgagcggg ccaaacacat ctggccttga gaccagggtt 420 gtgcaagcag agcggttctt caaaacccac ttgttcgatt gggtcactag tgactcgttc 480 ggacgatgcc acctgctgga acttccaact gaccacaaag gtgtctatgg cagcctgact 540 gattcttatg cgtacatgag aaacggttgg gatgtcgagg tcactgcggt gggaaaccag 600 ttcaacggag gatgcctgtt ggtggccatg gtgccagaac tctgctctat cagcaaaaga 660 gagctgtacc agctcacgct ctttccccac cagttcatca acccccggac gaacatgacg 720 gcacacatca ccgtgccctt tgtcggcgtc aaccgctacg accagtacaa ggtacacaaa 780 ccttggaccc tcgtggtcat ggtcgtggcc ccgctgactg tcaacactga aggtgctcca 840 cagatcaagg tttatgccaa catcgcccct accaacgtgc acgtcgcggg tgagttcccc 900 tccaaggaag ggatcttccc cgtggcctgc agtgacggtt acggcggtct tgtgaccact 960 gacccaaaga cggctgaccc cgtttacggg aaagttttca atccccctcg cagcatgttg 1020 ccagggcgat tcaccaactt ccttgatgtg gctgaggcgt gccctacgtt tctgcatttt 1080 gagggtgacg taccatacgt gaccacaaag acggattcgg acagggttct cgctcagttt 1140 gacttgtctt tggcagcgaa gcacatgtca aacacctttc ttgcaggtct cgcccagtac 1200 tacgcacagt acagtggcac tattaacctg cacttcatgt tcacagggcc aactgacgcg 1260 aaagcgcgtt acatgattgc ctatgccccc cccggcatgg agccgcctaa aacacctgag 1320 gcagccgctc actgcattca cgcggagtgg gatactggat tgaactcaaa attcacattc 1380 tcaatccctt acctctcggc ggctgactat gcgtacaccg cgtctgacac cgccgagacc 1440 acaaatgtac agggatgggt ttgcctgttt caaatcacgc acgggaaggc cgatggtgac 1500 gcacttgttg tcctggccag cgccggtaag gacttcgagc tgcggttgcc agttgatgcc 1560 cgcacgcaga ccacctccac aggtgagtca gctgaccccg tgactgccac tgttgagaac 1620 tacggtggcg aaacacaggt ccagagacgc cagcacacgg acgtctcgtt tatattggac 1680 agatttgtga aagtgacacc aaaagaccaa atcaatgtgt tggacctgat gcaaaccccc 1740 gcccacactt tggtaggtgc actcctccgc accgccacct actacttcgc agatttagag 1800 gtggcagtaa aacacgaggg gaaccttacc tgggtcccga atggggcgcc cgagacagcg 1860 ttggataaca ccactaatcc aacggcttac cacaaggcac cgctcactcg tcttgcactg 1920 ccttacacgg caccgcaccg tgtcttggct accgtttaca acgggaactg caagtacgac 1980 gagagcagca caaccaacgt gagaggagac ctgcaagtgt tggcccaaaa ggcggcgagg 2040 acgctgccca cctccttcaa ctacggtgcc atcaaagcta cacgggtgaa cgagttgctt 2100 taccgcatga agagggctga aacatactgc ccccggcctc ttttggccat tcatccgaat 2160 gaagcaagac acaagcaaaa gatagtggca cctgcgaagc aactcctgaa cttcgacctg 2220 ctcaagttgg cgggagacgt tgagtccaac cccgggccc 2259 <210> 377 <211> 2259 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(2259) <220> <221> misc_feature <222> (1)..(2259) <223> P1_O1_pan_Asia_wildtype <400> 377 ggt gct ggg caa tcc agc ccg gcg act ggg tca cag aac cag tca ggc 48 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 aac act gga agc atc atc aac aac tac tac atg cag cag tac cag aac 96 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 tcc atg gac aca caa ctt ggt gac aac gct atc agc gga ggc tct aac 144 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 gaa ggg tct aca gac acc acc tcc acc cac aca acc aac act cag aac 192 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Thr Asn Thr Gln Asn 50 55 60 aac gac tgg ttt tca aaa cta gcc agt tcc gct ttc agc ggt ctt ttc 240 Asn Asp Trp Phe Ser Lys Leu Ala Ser Ser Ala Phe Ser Gly Leu Phe 65 70 75 80 ggc gct ctt ctc gcc gac aag aaa acc gag gag act act ctt ctc gag 288 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 gac cgc atc ctc act acc cgt aac gga cac aca acc tcg aca acc cag 336 Asp Arg Ile Leu Thr Thr Arg Asn Gly His Thr Thr Ser Thr Thr Gln 100 105 110 tcg agc gtt gga gtc act tac ggg tac gca aca gcc gag gat ttt gtg 384 Ser Ser Val Gly Val Thr Tyr Gly Tyr Ala Thr Ala Glu Asp Phe Val 115 120 125 agc ggg cca aac aca tct ggc ctt gag acc agg gtt gtg caa gca gag 432 Ser Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Val Gln Ala Glu 130 135 140 cgg ttc ttc aaa acc cac ttg ttc gat tgg gtc act agt gac tcg ttc 480 Arg Phe Phe Lys Thr His Leu Phe Asp Trp Val Thr Ser Asp Ser Phe 145 150 155 160 gga cga tgc cac ctg ctg gaa ctt cca act gac cac aaa ggt gtc tac 528 Gly Arg Cys His Leu Leu Glu Leu Pro Thr Asp His Lys Gly Val Tyr 165 170 175 ggc agc ctg act gat tct tat gcg tac atg aga aac ggt tgg gat gtc 576 Gly Ser Leu Thr Asp Ser Tyr Ala Tyr Met Arg Asn Gly Trp Asp Val 180 185 190 gag gtc act gcg gtg gga aac cag ttc aac gga gga tgc ctg ttg gtg 624 Glu Val Thr Ala Val Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu Val 195 200 205 gcc atg gtg cca gaa ctc tgc tct atc agc aaa aga gag ctg tac cag 672 Ala Met Val Pro Glu Leu Cys Ser Ile Ser Lys Arg Glu Leu Tyr Gln 210 215 220 ctc acg ctc ttt ccc cac cag ttc atc aac ccc cgg acg aac atg acg 720 Leu Thr Leu Phe Pro His Gln Phe Ile Asn Pro Arg Thr Asn Met Thr 225 230 235 240 gca cac atc acc gtg ccc ttt gtc ggc gtc aac cgc tac gac cag tac 768 Ala His Ile Thr Val Pro Phe Val Gly Val Asn Arg Tyr Asp Gln Tyr 245 250 255 aag gta cac aaa cct tgg acc ctc gtg gtc atg gtc gtg gcc ccg ctg 816 Lys Val His Lys Pro Trp Thr Leu Val Val Met Val Val Ala Pro Leu 260 265 270 act gtc aac act gaa ggt gct cca cag atc aag gtt tat gcc aac atc 864 Thr Val Asn Thr Glu Gly Ala Pro Gln Ile Lys Val Tyr Ala Asn Ile 275 280 285 gcc cct acc aac gtg cac gtc gcg ggt gag ttc ccc tcc aag gaa ggg 912 Ala Pro Thr Asn Val His Val Ala Gly Glu Phe Pro Ser Lys Glu Gly 290 295 300 atc ttc ccc gtg gcc tgc agt gac ggt tac ggc ggt ctt gtg acc act 960 Ile Phe Pro Val Ala Cys Ser Asp Gly Tyr Gly Gly Leu Val Thr Thr 305 310 315 320 gac cca aag acg gct gac ccc gtt tac ggg aaa gtt ttc aat ccc cct 1008 Asp Pro Lys Thr Ala Asp Pro Val Tyr Gly Lys Val Phe Asn Pro Pro 325 330 335 cgc agc atg ttg cca ggg cga ttc acc aac ttc ctt gat gtg gct gag 1056 Arg Ser Met Leu Pro Gly Arg Phe Thr Asn Phe Leu Asp Val Ala Glu 340 345 350 gcg tgc cct acg ttt ctg cat ttt gag ggt gac gta cca tac gtg acc 1104 Ala Cys Pro Thr Phe Leu His Phe Glu Gly Asp Val Pro Tyr Val Thr 355 360 365 aca aag acg gat tcg gac agg gtt ctc gct cag ttt gac ttg tct ttg 1152 Thr Lys Thr Asp Ser Asp Arg Val Leu Ala Gln Phe Asp Leu Ser Leu 370 375 380 gca gcg aag cac atg tca aac acc ttt ctt gca ggt ctc gcc cag tac 1200 Ala Ala Lys His Met Ser Asn Thr Phe Leu Ala Gly Leu Ala Gln Tyr 385 390 395 400 tac gca cag tac agt ggc act att aac ctg cac ttc atg ttc aca ggg 1248 Tyr Ala Gln Tyr Ser Gly Thr Ile Asn Leu His Phe Met Phe Thr Gly 405 410 415 ccc act gac gcg aaa gcg cgt tac atg att gca tat gcc ccc ccc ggc 1296 Pro Thr Asp Ala Lys Ala Arg Tyr Met Ile Ala Tyr Ala Pro Pro Gly 420 425 430 atg gag ccg cct aaa aca cct gag gca gcc gct cac tgc att cac gcg 1344 Met Glu Pro Pro Lys Thr Pro Glu Ala Ala Ala His Cys Ile His Ala 435 440 445 gag tgg gat act gga ttg aat tca aaa ttc aca ttc tca atc cct tac 1392 Glu Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro Tyr 450 455 460 ctc tcg gcg gct gac tac gcg tac acc gcg tct gac acc gcc gag acc 1440 Leu Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Asp Thr Ala Glu Thr 465 470 475 480 aca aat gta cag gga tgg gtt tgc ctg ttt caa atc acg cac ggg aag 1488 Thr Asn Val Gln Gly Trp Val Cys Leu Phe Gln Ile Thr His Gly Lys 485 490 495 gcc gat ggt gac gca ctt gtt gtc ctg gct agc gcc ggt aag gac ttc 1536 Ala Asp Gly Asp Ala Leu Val Val Leu Ala Ser Ala Gly Lys Asp Phe 500 505 510 gag ctg cgg ttg cca gtt gat gcc cgc acg cag acc acc tcc aca ggt 1584 Glu Leu Arg Leu Pro Val Asp Ala Arg Thr Gln Thr Thr Ser Thr Gly 515 520 525 gag tca gct gac ccc gtg act gcc act gtt gag aac tac ggt ggc gaa 1632 Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu Asn Tyr Gly Gly Glu 530 535 540 aca cag gtc cag aga cgc cag cac acg gac gtc tcg ttt ata ttg gac 1680 Thr Gln Val Gln Arg Arg Gln His Thr Asp Val Ser Phe Ile Leu Asp 545 550 555 560 aga ttt gtg aaa gtg aca cca aaa gac caa atc aat gtg ttg gac ctg 1728 Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile Asn Val Leu Asp Leu 565 570 575 atg caa acc ccc gcc cac act ttg gta ggt gca ctc ctc cgc acc gcc 1776 Met Gln Thr Pro Ala His Thr Leu Val Gly Ala Leu Leu Arg Thr Ala 580 585 590 acc tac tac ttc gca gat tta gag gtg gca gta aaa cac gag ggg aac 1824 Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val Lys His Glu Gly Asn 595 600 605 ctt acc tgg gtc ccg aat ggg gcg ccc gag aca gcg ttg gat aac acc 1872 Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr Ala Leu Asp Asn Thr 610 615 620 act aat cca acg gct tac cac aag gca ccg ctc act cgt ctt gca ctg 1920 Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu Thr Arg Leu Ala Leu 625 630 635 640 cct tac acg gca ccg cac cgt gtc ttg gct acc gtt tac aac ggg aac 1968 Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr Val Tyr Asn Gly Asn 645 650 655 tgc aag tac gac gag agc agc aca acc aac gtg aga ggt gac ctg caa 2016 Cys Lys Tyr Asp Glu Ser Ser Thr Thr Asn Val Arg Gly Asp Leu Gln 660 665 670 gtg ttg gcc caa aag gcg gcg agg acg ctg ccc acc tcc ttc aac tac 2064 Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro Thr Ser Phe Asn Tyr 675 680 685 ggt gcc atc aaa gct acc cgg gtg aac gag ttg ctt tac cgc atg aag 2112 Gly Ala Ile Lys Ala Thr Arg Val Asn Glu Leu Leu Tyr Arg Met Lys 690 695 700 agg gct gaa aca tac tgc ccc cgg cct ctt ttg gcc att cat ccg aat 2160 Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu Ala Ile His Pro Asn 705 710 715 720 gaa gca aga cac aag caa aag ata gtg gca cct gcg aag caa ctc ctg 2208 Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Ala Lys Gln Leu Leu 725 730 735 aac ttc gac ctg ctc aag ttg gcg gga gac gtt gag tcc aac cct ggg 2256 Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 740 745 750 ccc 2259 Pro <210> 378 <211> 753 <212> PRT <213> Foot-and-mouth disease virus <400> 378 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Thr Asn Thr Gln Asn 50 55 60 Asn Asp Trp Phe Ser Lys Leu Ala Ser Ser Ala Phe Ser Gly Leu Phe 65 70 75 80 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 Asp Arg Ile Leu Thr Thr Arg Asn Gly His Thr Thr Ser Thr Thr Gln 100 105 110 Ser Ser Val Gly Val Thr Tyr Gly Tyr Ala Thr Ala Glu Asp Phe Val 115 120 125 Ser Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Val Gln Ala Glu 130 135 140 Arg Phe Phe Lys Thr His Leu Phe Asp Trp Val Thr Ser Asp Ser Phe 145 150 155 160 Gly Arg Cys His Leu Leu Glu Leu Pro Thr Asp His Lys Gly Val Tyr 165 170 175 Gly Ser Leu Thr Asp Ser Tyr Ala Tyr Met Arg Asn Gly Trp Asp Val 180 185 190 Glu Val Thr Ala Val Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu Val 195 200 205 Ala Met Val Pro Glu Leu Cys Ser Ile Ser Lys Arg Glu Leu Tyr Gln 210 215 220 Leu Thr Leu Phe Pro His Gln Phe Ile Asn Pro Arg Thr Asn Met Thr 225 230 235 240 Ala His Ile Thr Val Pro Phe Val Gly Val Asn Arg Tyr Asp Gln Tyr 245 250 255 Lys Val His Lys Pro Trp Thr Leu Val Val Met Val Val Ala Pro Leu 260 265 270 Thr Val Asn Thr Glu Gly Ala Pro Gln Ile Lys Val Tyr Ala Asn Ile 275 280 285 Ala Pro Thr Asn Val His Val Ala Gly Glu Phe Pro Ser Lys Glu Gly 290 295 300 Ile Phe Pro Val Ala Cys Ser Asp Gly Tyr Gly Gly Leu Val Thr Thr 305 310 315 320 Asp Pro Lys Thr Ala Asp Pro Val Tyr Gly Lys Val Phe Asn Pro Pro 325 330 335 Arg Ser Met Leu Pro Gly Arg Phe Thr Asn Phe Leu Asp Val Ala Glu 340 345 350 Ala Cys Pro Thr Phe Leu His Phe Glu Gly Asp Val Pro Tyr Val Thr 355 360 365 Thr Lys Thr Asp Ser Asp Arg Val Leu Ala Gln Phe Asp Leu Ser Leu 370 375 380 Ala Ala Lys His Met Ser Asn Thr Phe Leu Ala Gly Leu Ala Gln Tyr 385 390 395 400 Tyr Ala Gln Tyr Ser Gly Thr Ile Asn Leu His Phe Met Phe Thr Gly 405 410 415 Pro Thr Asp Ala Lys Ala Arg Tyr Met Ile Ala Tyr Ala Pro Pro Gly 420 425 430 Met Glu Pro Pro Lys Thr Pro Glu Ala Ala Ala His Cys Ile His Ala 435 440 445 Glu Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro Tyr 450 455 460 Leu Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Asp Thr Ala Glu Thr 465 470 475 480 Thr Asn Val Gln Gly Trp Val Cys Leu Phe Gln Ile Thr His Gly Lys 485 490 495 Ala Asp Gly Asp Ala Leu Val Val Leu Ala Ser Ala Gly Lys Asp Phe 500 505 510 Glu Leu Arg Leu Pro Val Asp Ala Arg Thr Gln Thr Thr Ser Thr Gly 515 520 525 Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu Asn Tyr Gly Gly Glu 530 535 540 Thr Gln Val Gln Arg Arg Gln His Thr Asp Val Ser Phe Ile Leu Asp 545 550 555 560 Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile Asn Val Leu Asp Leu 565 570 575 Met Gln Thr Pro Ala His Thr Leu Val Gly Ala Leu Leu Arg Thr Ala 580 585 590 Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val Lys His Glu Gly Asn 595 600 605 Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr Ala Leu Asp Asn Thr 610 615 620 Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu Thr Arg Leu Ala Leu 625 630 635 640 Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr Val Tyr Asn Gly Asn 645 650 655 Cys Lys Tyr Asp Glu Ser Ser Thr Thr Asn Val Arg Gly Asp Leu Gln 660 665 670 Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro Thr Ser Phe Asn Tyr 675 680 685 Gly Ala Ile Lys Ala Thr Arg Val Asn Glu Leu Leu Tyr Arg Met Lys 690 695 700 Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu Ala Ile His Pro Asn 705 710 715 720 Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Ala Lys Gln Leu Leu 725 730 735 Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 740 745 750 Pro <210> 379 <211> 2289 <212> DNA <213> Artificial Sequence <220> <223> P1_SAT1_KNP/196/91_cut_sites_removed <400> 379 ggagcgggcc agtcgtcccc cgccactggg tcacagaacc agtcaggcaa cactggcagc 60 atcatcaaca actactacat gcaacagtac caaaattcaa tggacaccca actcggagac 120 aacgccatct ccggtgggtc aaatgagggt tcgaccgaca caacttcgac ccacaccagc 180 aacacccaaa acaacgattg gttttcaaaa ttggctcaat cggccttctc tggattggtt 240 ggtgcgttgc ttgccgacaa gaagactgag gaaaccacac tccttgagga tcgcatcctc 300 acgaccagtc acggcacaac cacctcgacc acacaaagtt cagtgggcat aacctacggt 360 tacgccgact cggaccgttt cctccccggc ccaaacacca acgggctgga gacacgtgtg 420 gaacaagcag agaggttttt caaacacaaa ttatttgatt ggacacttga acaacgattt 480 ggaacaacac acgttttgga actgcccaca gaccacaaag gcatctatgg acaacttgtt 540 gactcccact catacattcg caatgggtgg gacgttgagg tctccgcgac cgcaacgcag 600 ttcaacgggg gctgcctcct ggtagcgatg gtgcccgaat tgtgcaaact gtcggaaaga 660 gagaaatacc aactcactct cttccctcat caatttctag acccaaggac caacaccacg 720 gcacacatcc aggttcccta cctgggcgtg gatcgccacg accaaggaac acgccacaaa 780 gcgtggaccc tggttgtcat ggtggtggca ccctacacaa acgatcagac gattggctcg 840 aacaaagccg aggtgtacgt gaacattgct cccacaaacg tttacgtcgc cggtgagaag 900 cccgcaaaac agggcattct ccccgtggcc gtctccgttg gctatggtgg cttccaaaat 960 acagatccca aaacatcgga ccccgtatat gggcacgtgt acaacccggc tcgcaccggc 1020 ctacctggga ggttcacaaa cctcttggat gtggctgaag cgtgccctac actgcttgac 1080 ttcaacggag ttccgtatgt gaccacccag gcaaactcag gatctaaagt gttaacttgt 1140 tttgatttgg cttttggtca caaaaatttg aaaaatacat ttatgtctgg tcttgcccag 1200 tactacaccc agtacagtgg cacactcaac ctgcacttca tgtacacagg cccaaccaac 1260 aacaaggcta agtacatggt ggcctacatc ccacctggga cacaccctct cccggaaaca 1320 ccggagatgg agtcccactg tcaccacgcc gagtgggaca caggcctgaa ctcaaccttc 1380 acattcaccg tgccgtatgt gtcggctgcc gacttcgcgt acacctactc tgacgagcct 1440 gaacaggctt cggttcaggg ttgggtgggc gtatatcagg tgactgacac acacgagaag 1500 gacggtgcag ttgttgtgtc cgtcagtgct ggacccgact tcgagttcag gatgcccatc 1560 agcccctcgc gccagaccac atccgctggc gaaggagcgg agcctgtcac gactgatgct 1620 tcccaacacg gaggcgacag acggacaact cgtaggcatc acactgatgt gagcttcttg 1680 ctcgatcggt tcacactggt tggtaaaaca caggacaaca aactgacact agacctgctc 1740 caaaccaagg aaaaagcact ggttggcgca atcctgcgcg cagccacgta ctacttctct 1800 gacttggagg ttgcgtgtgt gggtgacaac aaatgggtcg gctggactcc caacggtgct 1860 ccagaacttg cggaagtggg cgacaaccca gtcgtctttt ccaaaggtag aaccacccgt 1920 tttgcactgc cctacaccgc tccacacagg tgcttggcga cagcctacaa cggtgactgc 1980 aagtacaaac ccactggcac agctccacgc gaaaacattc gtggagacct cgcaactctc 2040 gcggcgagga ttgcaagtga gacacacatt ccaaccacct tcaactatgg caggatttac 2100 acagacactg aggtcgatgt gtacgtcagg atgaagcgcg cggaactcta ctgcccgcga 2160 cccgttctca cgcactacga ccacggtggc agggatcgct acagaactgc gatcaccaaa 2220 cctgtcaaac agatgtgcag tttcgacctg ttaaagctgg ctggagacgt tgagtccaac 2280 cctgggccc 2289 <210> 380 <211> 2289 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(2289) <220> <221> misc_feature <222> (1)..(2289) <223> P1_SAT1_KNP/196/91_wildtype <400> 380 gga gcg ggc cag tcg tcc ccc gcc act ggg tcc cag aac cag tct ggc 48 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 aac act ggc agc atc atc aac aac tac tac atg caa cag tac caa aat 96 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 tcc atg gac acc caa ctc gga gac aac gcc atc tcc ggt ggg tca aat 144 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 gag ggt tcg acc gac aca acg tcg acc cac acc agc aac acc caa aac 192 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Ser Asn Thr Gln Asn 50 55 60 aac gat tgg ttt tca aaa ttg gct caa tcg gcc ttc tct gga ttg gtt 240 Asn Asp Trp Phe Ser Lys Leu Ala Gln Ser Ala Phe Ser Gly Leu Val 65 70 75 80 ggt gcg ttg ctt gcc gac aag aag act gag gaa acc aca ctc ctc gag 288 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 gat cgc atc ctc acg acc agt cac ggc aca acc acc tcg acc aca caa 336 Asp Arg Ile Leu Thr Thr Ser His Gly Thr Thr Thr Ser Thr Thr Gln 100 105 110 agt tca gtg ggc ata acc tac ggt tac gcc gac tcg gac cgt ttc ctc 384 Ser Ser Val Gly Ile Thr Tyr Gly Tyr Ala Asp Ser Asp Arg Phe Leu 115 120 125 ccc ggc cca aac acc aac ggg ctg gag aca cgt gtg gaa caa gca gag 432 Pro Gly Pro Asn Thr Asn Gly Leu Glu Thr Arg Val Glu Gln Ala Glu 130 135 140 agg ttt ttc aaa cac aaa tta ttt gat tgg aca ctt gaa caa cga ttt 480 Arg Phe Phe Lys His Lys Leu Phe Asp Trp Thr Leu Glu Gln Arg Phe 145 150 155 160 gga aca aca cac gtt ttg gaa ctg ccc aca gac cac aaa ggc atc tat 528 Gly Thr Thr His Val Leu Glu Leu Pro Thr Asp His Lys Gly Ile Tyr 165 170 175 gga caa ctt gtt gac tcc cac tca tac att cgc aat ggg tgg gac gtt 576 Gly Gln Leu Val Asp Ser His Ser Tyr Ile Arg Asn Gly Trp Asp Val 180 185 190 gag gtc tcc gcg acc gca acg cag ttc aac ggg ggc tgc ctc ctg gta 624 Glu Val Ser Ala Thr Ala Thr Gln Phe Asn Gly Gly Cys Leu Leu Val 195 200 205 gcg atg gtg ccc gaa ttg tgc aaa ctg tcg gaa aga gag aaa tac caa 672 Ala Met Val Pro Glu Leu Cys Lys Leu Ser Glu Arg Glu Lys Tyr Gln 210 215 220 ctc act ctc ttc cct cat caa ttt ctc gac cca agg acc aac acc acg 720 Leu Thr Leu Phe Pro His Gln Phe Leu Asp Pro Arg Thr Asn Thr Thr 225 230 235 240 gca cac atc cag gta ccc tac ctg ggc gtg gat cgc cac gac caa gga 768 Ala His Ile Gln Val Pro Tyr Leu Gly Val Asp Arg His Asp Gln Gly 245 250 255 aca cgc cac aaa gcg tgg acc ctg gtt gtc atg gtg gtg gca ccc tac 816 Thr Arg His Lys Ala Trp Thr Leu Val Val Met Val Val Ala Pro Tyr 260 265 270 aca aac gat cag acg att ggc tcg aac aaa gcc gag gtg tac gtg aac 864 Thr Asn Asp Gln Thr Ile Gly Ser Asn Lys Ala Glu Val Tyr Val Asn 275 280 285 att gct ccc aca aac gtt tac gtc gcc ggt gag aag ccc gca aaa cag 912 Ile Ala Pro Thr Asn Val Tyr Val Ala Gly Glu Lys Pro Ala Lys Gln 290 295 300 ggc att ctc ccc gtg gcc gtc tcc gtt ggc tat ggt ggc ttc caa aat 960 Gly Ile Leu Pro Val Ala Val Ser Val Gly Tyr Gly Gly Phe Gln Asn 305 310 315 320 aca gat ccc aaa aca tcg gac ccc gta tac ggg cac gtg tac aac ccg 1008 Thr Asp Pro Lys Thr Ser Asp Pro Val Tyr Gly His Val Tyr Asn Pro 325 330 335 gct cgc acc ggc cta cct ggg agg ttc aca aac ctc ttg gat gtg gct 1056 Ala Arg Thr Gly Leu Pro Gly Arg Phe Thr Asn Leu Leu Asp Val Ala 340 345 350 gaa gcg tgc cct aca ctg ctt gac ttc aac gga gtt ccg tac gtg acc 1104 Glu Ala Cys Pro Thr Leu Leu Asp Phe Asn Gly Val Pro Tyr Val Thr 355 360 365 acc cag gca aac tct gga tct aaa gtg tta act tgt ttt gat ttg gct 1152 Thr Gln Ala Asn Ser Gly Ser Lys Val Leu Thr Cys Phe Asp Leu Ala 370 375 380 ttt ggt cac aaa aat ttg aaa aat aca ttt atg tct ggt ctt gcc cag 1200 Phe Gly His Lys Asn Leu Lys Asn Thr Phe Met Ser Gly Leu Ala Gln 385 390 395 400 tac tac acc cag tac agt ggc aca ctc aac ctg cac ttc atg tac aca 1248 Tyr Tyr Thr Gln Tyr Ser Gly Thr Leu Asn Leu His Phe Met Tyr Thr 405 410 415 ggc cca acc aac aac aag gct aag tac atg gtg gcc tac atc cca cct 1296 Gly Pro Thr Asn Asn Lys Ala Lys Tyr Met Val Ala Tyr Ile Pro Pro 420 425 430 ggg aca cac cct ctc ccg gaa aca ccg gag atg gag tcc cac tgt cac 1344 Gly Thr His Pro Leu Pro Glu Thr Pro Glu Met Glu Ser His Cys His 435 440 445 cac gcc gag tgg gac aca ggc ctg aac tca acc ttc aca ttc acc gtg 1392 His Ala Glu Trp Asp Thr Gly Leu Asn Ser Thr Phe Thr Phe Thr Val 450 455 460 ccg tac gtg tcg gct gcc gac ttc gcg tac acc tac tct gac gag cct 1440 Pro Tyr Val Ser Ala Ala Asp Phe Ala Tyr Thr Tyr Ser Asp Glu Pro 465 470 475 480 gaa cag gct tcg gtt cag ggt tgg gtg ggc gta tac cag gtg act gac 1488 Glu Gln Ala Ser Val Gln Gly Trp Val Gly Val Tyr Gln Val Thr Asp 485 490 495 aca cac gag aag gac ggt gca gtt gtt gtg tcc gtc agt gct gga ccc 1536 Thr His Glu Lys Asp Gly Ala Val Val Val Ser Val Ser Ala Gly Pro 500 505 510 gac ttc gag ttc agg atg ccc atc agc ccc tcg cgc cag acc aca tcc 1584 Asp Phe Glu Phe Arg Met Pro Ile Ser Pro Ser Arg Gln Thr Thr Ser 515 520 525 gct ggc gaa gga gcg gag cct gtc acg act gat gct tcc caa cac gga 1632 Ala Gly Glu Gly Ala Glu Pro Val Thr Thr Asp Ala Ser Gln His Gly 530 535 540 ggc gac aga cgg aca act cgt agg cat cac act gat gtg agc ttc ttg 1680 Gly Asp Arg Arg Thr Thr Arg Arg His His Thr Asp Val Ser Phe Leu 545 550 555 560 ctc gac cgg ttc aca ctg gtt ggt aaa aca cag gac aac aaa ctg aca 1728 Leu Asp Arg Phe Thr Leu Val Gly Lys Thr Gln Asp Asn Lys Leu Thr 565 570 575 cta gac ctg ctc caa acc aag gaa aaa gca ctg gtt ggc gca atc ctg 1776 Leu Asp Leu Leu Gln Thr Lys Glu Lys Ala Leu Val Gly Ala Ile Leu 580 585 590 cgc gca gcc acg tac tac ttc tct gac ttg gag gtt gcg tgt gtg ggt 1824 Arg Ala Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Val Ala Cys Val Gly 595 600 605 gac aac aaa tgg gtc ggc tgg act ccc aac gga gct cca gaa ctt gcg 1872 Asp Asn Lys Trp Val Gly Trp Thr Pro Asn Gly Ala Pro Glu Leu Ala 610 615 620 gaa gtg ggc gac aac cca gtc gtc ttt tcc aaa ggt aga acc acc cgt 1920 Glu Val Gly Asp Asn Pro Val Val Phe Ser Lys Gly Arg Thr Thr Arg 625 630 635 640 ttt gca ctg ccc tac acc gct cca cac agg tgc ttg gcg aca gcc tac 1968 Phe Ala Leu Pro Tyr Thr Ala Pro His Arg Cys Leu Ala Thr Ala Tyr 645 650 655 aac ggt gac tgc aag tac aaa ccc act ggc aca gct cca cgc gaa aac 2016 Asn Gly Asp Cys Lys Tyr Lys Pro Thr Gly Thr Ala Pro Arg Glu Asn 660 665 670 att cgt gga gac ctc gca act ctc gcg gcg agg att gca agt gag aca 2064 Ile Arg Gly Asp Leu Ala Thr Leu Ala Ala Arg Ile Ala Ser Glu Thr 675 680 685 cac att cca acc acc ttc aac tat ggc agg att tac aca gac act gag 2112 His Ile Pro Thr Thr Phe Asn Tyr Gly Arg Ile Tyr Thr Asp Thr Glu 690 695 700 gtc gac gtg tac gtc agg atg aag cgc gcg gag ctc tac tgc ccg cga 2160 Val Asp Val Tyr Val Arg Met Lys Arg Ala Glu Leu Tyr Cys Pro Arg 705 710 715 720 ccc gtt ctc acg cac tac gac cac ggt ggc agg gat cgc tac aga act 2208 Pro Val Leu Thr His Tyr Asp His Gly Gly Arg Asp Arg Tyr Arg Thr 725 730 735 gcg atc acc aaa cct gtc aaa cag atg tgc agt ttc gac ctg tta aag 2256 Ala Ile Thr Lys Pro Val Lys Gln Met Cys Ser Phe Asp Leu Leu Lys 740 745 750 ctg gct gga gac gtt gag tcc aac cct ggg ccc 2289 Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 755 760 <210> 381 <211> 763 <212> PRT <213> Foot-and-mouth disease virus <400> 381 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Ser Asn Thr Gln Asn 50 55 60 Asn Asp Trp Phe Ser Lys Leu Ala Gln Ser Ala Phe Ser Gly Leu Val 65 70 75 80 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 Asp Arg Ile Leu Thr Thr Ser His Gly Thr Thr Thr Ser Thr Thr Gln 100 105 110 Ser Ser Val Gly Ile Thr Tyr Gly Tyr Ala Asp Ser Asp Arg Phe Leu 115 120 125 Pro Gly Pro Asn Thr Asn Gly Leu Glu Thr Arg Val Glu Gln Ala Glu 130 135 140 Arg Phe Phe Lys His Lys Leu Phe Asp Trp Thr Leu Glu Gln Arg Phe 145 150 155 160 Gly Thr Thr His Val Leu Glu Leu Pro Thr Asp His Lys Gly Ile Tyr 165 170 175 Gly Gln Leu Val Asp Ser His Ser Tyr Ile Arg Asn Gly Trp Asp Val 180 185 190 Glu Val Ser Ala Thr Ala Thr Gln Phe Asn Gly Gly Cys Leu Leu Val 195 200 205 Ala Met Val Pro Glu Leu Cys Lys Leu Ser Glu Arg Glu Lys Tyr Gln 210 215 220 Leu Thr Leu Phe Pro His Gln Phe Leu Asp Pro Arg Thr Asn Thr Thr 225 230 235 240 Ala His Ile Gln Val Pro Tyr Leu Gly Val Asp Arg His Asp Gln Gly 245 250 255 Thr Arg His Lys Ala Trp Thr Leu Val Val Met Val Val Ala Pro Tyr 260 265 270 Thr Asn Asp Gln Thr Ile Gly Ser Asn Lys Ala Glu Val Tyr Val Asn 275 280 285 Ile Ala Pro Thr Asn Val Tyr Val Ala Gly Glu Lys Pro Ala Lys Gln 290 295 300 Gly Ile Leu Pro Val Ala Val Ser Val Gly Tyr Gly Gly Phe Gln Asn 305 310 315 320 Thr Asp Pro Lys Thr Ser Asp Pro Val Tyr Gly His Val Tyr Asn Pro 325 330 335 Ala Arg Thr Gly Leu Pro Gly Arg Phe Thr Asn Leu Leu Asp Val Ala 340 345 350 Glu Ala Cys Pro Thr Leu Leu Asp Phe Asn Gly Val Pro Tyr Val Thr 355 360 365 Thr Gln Ala Asn Ser Gly Ser Lys Val Leu Thr Cys Phe Asp Leu Ala 370 375 380 Phe Gly His Lys Asn Leu Lys Asn Thr Phe Met Ser Gly Leu Ala Gln 385 390 395 400 Tyr Tyr Thr Gln Tyr Ser Gly Thr Leu Asn Leu His Phe Met Tyr Thr 405 410 415 Gly Pro Thr Asn Asn Lys Ala Lys Tyr Met Val Ala Tyr Ile Pro Pro 420 425 430 Gly Thr His Pro Leu Pro Glu Thr Pro Glu Met Glu Ser His Cys His 435 440 445 His Ala Glu Trp Asp Thr Gly Leu Asn Ser Thr Phe Thr Phe Thr Val 450 455 460 Pro Tyr Val Ser Ala Ala Asp Phe Ala Tyr Thr Tyr Ser Asp Glu Pro 465 470 475 480 Glu Gln Ala Ser Val Gln Gly Trp Val Gly Val Tyr Gln Val Thr Asp 485 490 495 Thr His Glu Lys Asp Gly Ala Val Val Val Ser Val Ser Ala Gly Pro 500 505 510 Asp Phe Glu Phe Arg Met Pro Ile Ser Pro Ser Arg Gln Thr Thr Ser 515 520 525 Ala Gly Glu Gly Ala Glu Pro Val Thr Thr Asp Ala Ser Gln His Gly 530 535 540 Gly Asp Arg Arg Thr Thr Arg Arg His His Thr Asp Val Ser Phe Leu 545 550 555 560 Leu Asp Arg Phe Thr Leu Val Gly Lys Thr Gln Asp Asn Lys Leu Thr 565 570 575 Leu Asp Leu Leu Gln Thr Lys Glu Lys Ala Leu Val Gly Ala Ile Leu 580 585 590 Arg Ala Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Val Ala Cys Val Gly 595 600 605 Asp Asn Lys Trp Val Gly Trp Thr Pro Asn Gly Ala Pro Glu Leu Ala 610 615 620 Glu Val Gly Asp Asn Pro Val Val Phe Ser Lys Gly Arg Thr Thr Arg 625 630 635 640 Phe Ala Leu Pro Tyr Thr Ala Pro His Arg Cys Leu Ala Thr Ala Tyr 645 650 655 Asn Gly Asp Cys Lys Tyr Lys Pro Thr Gly Thr Ala Pro Arg Glu Asn 660 665 670 Ile Arg Gly Asp Leu Ala Thr Leu Ala Ala Arg Ile Ala Ser Glu Thr 675 680 685 His Ile Pro Thr Thr Phe Asn Tyr Gly Arg Ile Tyr Thr Asp Thr Glu 690 695 700 Val Asp Val Tyr Val Arg Met Lys Arg Ala Glu Leu Tyr Cys Pro Arg 705 710 715 720 Pro Val Leu Thr His Tyr Asp His Gly Gly Arg Asp Arg Tyr Arg Thr 725 730 735 Ala Ile Thr Lys Pro Val Lys Gln Met Cys Ser Phe Asp Leu Leu Lys 740 745 750 Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 755 760 <210> 382 <211> 2277 <212> DNA <213> Artificial Sequence <220> <223> P1_SAT2_Egypt_2010_cut_sites_removed <400> 382 ggggccgggc aatccagccc ggctactggt tcccaaaatc aatcaggcaa tactggtagc 60 attattaaca actactacat gcaacagtac cagaactcaa tggacacaca gcttggtgac 120 aacgccatta gcggtggttc caacgagggg tcaacggaca ccacgtcaac acacacaaac 180 aacacacaga acaatgactg gttttccaaa ttggctcaat cagccatttc agggcttttc 240 ggtgctctgc ttgcagacaa gaaaacagag gagaccacac tgctggagga cagaatcctg 300 accacacgtc acgggaccac gacctccacc acgcaaagtt ctgtgggagt gacatttggt 360 tatgctgatg ctgattcgtt tcgcccagga ccaaacacct ctgggcttga gacgcgcgtg 420 caacaggcag aacgcttctt caaggagaaa ctttttgact ggaccagtga caaacctttc 480 ggtacgctgt acgtgttgga gttgcccaaa gaccacaagg gcatctacgg taaacttacc 540 gactcctaca cgtacatgcg taacggttgg gacgttcagg tcagcgcgac cagcacacag 600 tttaacggcg gatcactgct cgtggcaatg gtcccagagt tgtgcagtct aaaagccaga 660 gaagaatttc aactcactct ctacccacac cagttcatta acccgcgcac caacacaacc 720 gcacacatac aggttcctta cctgggtgta aacagacacg accagggtaa gcgccaccag 780 gcgtggtctc tggttgtgat ggtgctcacg cctcttacca ccgaggcgca gatgaacagc 840 ggcaccgtcg aggtgtacgc caacatcgca ccaacaaatg tggttgtggc gggtgaactg 900 ccgggtaagc agggcatcat tccggtcgct gccgctgacg ggtatggtgg tttccagaac 960 accgacccga aaacggctga ccccatttac gggcatgtgt acaacccgtc cagaaacgac 1020 tgccacgggc gatactccaa tcttatggat gtcgccgagg cgtgtccaac actcctcaac 1080 tttgatggca agccctacgt cgtgaccaag aacaacggtg acaaggtgat ggcacgcttc 1140 gatgtcgcct ttacacacaa ggtgcatggg aacacgtttc tggcgggctt ggccgactac 1200 tacacacagt attcaggcag cctaaactac cacttcatgt acactggacc cacacatcac 1260 aaggcaaagt tcatggtggc atacgtgccc cctggtgttg cagttgacca actgcctagc 1320 acaccggagg atgctgcgca ctgctaccat gcggaatggg acaccgggtt gaactcttct 1380 ttctcgttcg cagtgcctta catctccgct gcggactttt cttacacaca cacagacaca 1440 ccagcaatgg ccaccactaa tggctgggta gttgtactac aggtcacaga cacgcactct 1500 gcggaagctg ccgttgtagt gtcagtcagc gcgggaccag atttggagtt ccgattccct 1560 atcgaccctg tgcgacagac tacctcggcg ggagaaggcg cagatgttgt caccacggac 1620 ccatcgacac acggtgggaa tgttcaagag ggacgacgca aacacaccga cgttgcgttc 1680 cttcttgatc gcagtacaca cgttcacaca aataagacat cttttgttgt ggacctcatg 1740 gacacaaggg agaaggcgct cgtaggcgca atcctgcgag catccaccta ctacttttgt 1800 gaccttgaaa ttgcatgtgt gggcgaccac acaagggtct tctggcagcc caacggggca 1860 ccacggacta cccagctcgg cgacaaccct atggtttttg ccaagggcgg cgtaacccgc 1920 tttgccatcc cgtttacggc tccacacagg ctgctatcta ctgtttacaa cggcgagtgt 1980 gtgtacacca aggccgccgc tgccattcgt ggtgatcgtg cggcacttgc ggcaaagtac 2040 gctgacacca accacacttt gccgccaacc ttcaacttcg ggtacgtgac cgttgacaaa 2100 ccagttgacg tttactaccg gatgaagagg gctgagctgt actgcccacg cccactgctg 2160 ccagcctaca aacacacaga cagagacaga ttcgacgcgc ccatcggcgt cgaaagacag 2220 accctgaatt ttgacctgct gaaactggca ggagacgttg agtccaaccc tgggccc 2277 <210> 383 <211> 2277 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(2277) <220> <221> misc_feature <222> (1)..(2277) <223> P1_SAT2_Egypt_2010_wildtype <400> 383 ggg gcc ggg caa tcc agc ccg gct act gga tcc caa aat caa tca ggc 48 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 aat act ggt agc att att aac aac tac tac atg caa cag tac cag aac 96 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 tca atg gac aca cag ctt ggt gac aac gcc att agc ggt ggt tcc aac 144 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 gag ggg tcg acg gac acc acg tcg aca cac aca aac aac aca cag aac 192 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Asn Asn Thr Gln Asn 50 55 60 aat gac tgg ttt tcc aaa ttg gct caa tca gcc att tca ggg ctt ttc 240 Asn Asp Trp Phe Ser Lys Leu Ala Gln Ser Ala Ile Ser Gly Leu Phe 65 70 75 80 gga gct ctg ctt gca gac aag aaa aca gag gag acc aca ctg ctg gag 288 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 gac aga atc ctg acc acg cgt cac ggg acc acg acc tcc acc acg caa 336 Asp Arg Ile Leu Thr Thr Arg His Gly Thr Thr Thr Ser Thr Thr Gln 100 105 110 agt tct gtg gga gtg aca ttt ggt tat gct gat gct gat tcg ttt cgc 384 Ser Ser Val Gly Val Thr Phe Gly Tyr Ala Asp Ala Asp Ser Phe Arg 115 120 125 cca gga ccc aac acc tct ggg ctt gag acg cgc gtg caa cag gca gaa 432 Pro Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Gln Gln Ala Glu 130 135 140 cgc ttc ttc aag gag aag ctt ttt gac tgg acc agt gac aaa cct ttc 480 Arg Phe Phe Lys Glu Lys Leu Phe Asp Trp Thr Ser Asp Lys Pro Phe 145 150 155 160 ggt acg ctg tac gtg ttg gag ttg ccc aaa gac cac aag ggc atc tac 528 Gly Thr Leu Tyr Val Leu Glu Leu Pro Lys Asp His Lys Gly Ile Tyr 165 170 175 ggt aaa ctt acc gac tcc tac acg tac atg cgt aac ggt tgg gac gtt 576 Gly Lys Leu Thr Asp Ser Tyr Thr Tyr Met Arg Asn Gly Trp Asp Val 180 185 190 cag gtc agc gcg acc agc aca cag ttt aac ggc gga tca ctg ctc gtg 624 Gln Val Ser Ala Thr Ser Thr Gln Phe Asn Gly Gly Ser Leu Leu Val 195 200 205 gca atg gtc cca gag ttg tgc agt cta aaa gcc aga gaa gaa ttt caa 672 Ala Met Val Pro Glu Leu Cys Ser Leu Lys Ala Arg Glu Glu Phe Gln 210 215 220 ctc act ctc tac cca cac cag ttc att aac ccg cgc acc aac aca acc 720 Leu Thr Leu Tyr Pro His Gln Phe Ile Asn Pro Arg Thr Asn Thr Thr 225 230 235 240 gca cac ata cag gtt cct tac ctg ggt gta aac aga cac gac cag ggt 768 Ala His Ile Gln Val Pro Tyr Leu Gly Val Asn Arg His Asp Gln Gly 245 250 255 aag cgc cac cag gcg tgg tct ctg gtt gtg atg gtg ctc acg cct ctt 816 Lys Arg His Gln Ala Trp Ser Leu Val Val Met Val Leu Thr Pro Leu 260 265 270 acc acc gag gcg cag atg aac agc ggc acc gtc gag gtg tac gcc aac 864 Thr Thr Glu Ala Gln Met Asn Ser Gly Thr Val Glu Val Tyr Ala Asn 275 280 285 atc gca cca aca aat gtg gtt gtg gcg ggt gaa ctg ccg ggt aag cag 912 Ile Ala Pro Thr Asn Val Val Val Ala Gly Glu Leu Pro Gly Lys Gln 290 295 300 ggc atc att ccg gtc gct gcc gct gac ggg tat ggt ggt ttc cag aac 960 Gly Ile Ile Pro Val Ala Ala Ala Asp Gly Tyr Gly Gly Phe Gln Asn 305 310 315 320 acc gac ccg aaa acg gct gac ccc att tac ggg cat gtg tac aac ccg 1008 Thr Asp Pro Lys Thr Ala Asp Pro Ile Tyr Gly His Val Tyr Asn Pro 325 330 335 tcc aga aac gac tgc cac ggg cga tac tcc aat ctt atg gat gtc gcc 1056 Ser Arg Asn Asp Cys His Gly Arg Tyr Ser Asn Leu Met Asp Val Ala 340 345 350 gag gcg tgt cca aca ctc ctc aac ttt gat ggc aag ccc tac gtc gtg 1104 Glu Ala Cys Pro Thr Leu Leu Asn Phe Asp Gly Lys Pro Tyr Val Val 355 360 365 acc aag aac aac ggt gac aag gtg atg gca cgc ttc gac gtc gcc ttt 1152 Thr Lys Asn Asn Gly Asp Lys Val Met Ala Arg Phe Asp Val Ala Phe 370 375 380 aca cac aag gtg cat ggg aac acg ttt ctg gcg ggc ttg gcc gac tac 1200 Thr His Lys Val His Gly Asn Thr Phe Leu Ala Gly Leu Ala Asp Tyr 385 390 395 400 tac aca cag tat tca ggc agc cta aac tac cac ttc atg tac act gga 1248 Tyr Thr Gln Tyr Ser Gly Ser Leu Asn Tyr His Phe Met Tyr Thr Gly 405 410 415 ccc aca cat cac aag gca aag ttc atg gtg gca tac gtg ccc cct ggt 1296 Pro Thr His His Lys Ala Lys Phe Met Val Ala Tyr Val Pro Pro Gly 420 425 430 gtt gca gtt gac cag ctg cct agc aca ccg gag gat gct gcg cac tgc 1344 Val Ala Val Asp Gln Leu Pro Ser Thr Pro Glu Asp Ala Ala His Cys 435 440 445 tac cat gcg gaa tgg gac acc ggg ttg aat tct tct ttc tcg ttc gca 1392 Tyr His Ala Glu Trp Asp Thr Gly Leu Asn Ser Ser Phe Ser Phe Ala 450 455 460 gtg cct tac atc tcc gct gcg gac ttt tct tac aca cac aca gac aca 1440 Val Pro Tyr Ile Ser Ala Ala Asp Phe Ser Tyr Thr His Thr Asp Thr 465 470 475 480 ccg gcc atg gcc acc acc aat ggc tgg gtg gtt gta ctg cag gtc acc 1488 Pro Ala Met Ala Thr Thr Asn Gly Trp Val Val Val Leu Gln Val Thr 485 490 495 gac acg cac tct gcg gaa gct gcc gtt gta gtg tca gtc agc gcg gga 1536 Asp Thr His Ser Ala Glu Ala Ala Val Val Val Ser Val Ser Ala Gly 500 505 510 cca gat ttg gaa ttc cga ttc cct atc gac cct gtg cga cag act acc 1584 Pro Asp Leu Glu Phe Arg Phe Pro Ile Asp Pro Val Arg Gln Thr Thr 515 520 525 tcg gcg gga gaa ggc gca gat gtt gtc acc acg gac cca tcg aca cac 1632 Ser Ala Gly Glu Gly Ala Asp Val Val Thr Thr Asp Pro Ser Thr His 530 535 540 ggt ggg aat gtt caa gag ggt cga cgc aaa cac acc gac gtt gcg ttc 1680 Gly Gly Asn Val Gln Glu Gly Arg Arg Lys His Thr Asp Val Ala Phe 545 550 555 560 ctt ctt gat cgc agt aca cac gtt cac aca aat aag aca tct ttt gtt 1728 Leu Leu Asp Arg Ser Thr His Val His Thr Asn Lys Thr Ser Phe Val 565 570 575 gtg gac ctc atg gac aca agg gag aag gcg ctc gta ggc gca atc ctg 1776 Val Asp Leu Met Asp Thr Arg Glu Lys Ala Leu Val Gly Ala Ile Leu 580 585 590 cga gca tcc acc tac tac ttt tgt gac ctt gaa att gca tgt gtg ggc 1824 Arg Ala Ser Thr Tyr Tyr Phe Cys Asp Leu Glu Ile Ala Cys Val Gly 595 600 605 gac cac aca agg gtc ttc tgg cag ccc aac ggg gca ccg cgg act acc 1872 Asp His Thr Arg Val Phe Trp Gln Pro Asn Gly Ala Pro Arg Thr Thr 610 615 620 cag ctc ggc gac aac cct atg gtt ttt gcc aag ggc ggc gta acc cgc 1920 Gln Leu Gly Asp Asn Pro Met Val Phe Ala Lys Gly Gly Val Thr Arg 625 630 635 640 ttt gcc atc ccg ttt acg gct cca cac agg ctg ctg tct act gtt tac 1968 Phe Ala Ile Pro Phe Thr Ala Pro His Arg Leu Leu Ser Thr Val Tyr 645 650 655 aac ggc gag tgt gtc tac acc aag gcc gcc gct gcc att cgt ggt gat 2016 Asn Gly Glu Cys Val Tyr Thr Lys Ala Ala Ala Ala Ile Arg Gly Asp 660 665 670 cgt gcg gca ctt gcg gca aag tac gct gac acc aac cac act ttg ccg 2064 Arg Ala Ala Leu Ala Ala Lys Tyr Ala Asp Thr Asn His Thr Leu Pro 675 680 685 cca acc ttc aac ttc ggg tac gtg acc gtt gac aaa cca gtc gac gtt 2112 Pro Thr Phe Asn Phe Gly Tyr Val Thr Val Asp Lys Pro Val Asp Val 690 695 700 tac tac cgg atg aag agg gct gag ctg tac tgc cca cgc cca ctg ctg 2160 Tyr Tyr Arg Met Lys Arg Ala Glu Leu Tyr Cys Pro Arg Pro Leu Leu 705 710 715 720 cca gcc tac aaa cac aca gac aga gac aga ttc gac gcg ccc atc ggc 2208 Pro Ala Tyr Lys His Thr Asp Arg Asp Arg Phe Asp Ala Pro Ile Gly 725 730 735 gtc gaa aga cag acc ctg aat ttt gac ctg ctg aaa ctg gca gga gac 2256 Val Glu Arg Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp 740 745 750 gtt gag tcc aac cct ggg ccc 2277 Val Glu Ser Asn Pro Gly Pro 755 <210> 384 <211> 759 <212> PRT <213> Foot-and-mouth disease virus <400> 384 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Asn Asn Thr Gln Asn 50 55 60 Asn Asp Trp Phe Ser Lys Leu Ala Gln Ser Ala Ile Ser Gly Leu Phe 65 70 75 80 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 Asp Arg Ile Leu Thr Thr Arg His Gly Thr Thr Thr Ser Thr Thr Gln 100 105 110 Ser Ser Val Gly Val Thr Phe Gly Tyr Ala Asp Ala Asp Ser Phe Arg 115 120 125 Pro Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Gln Gln Ala Glu 130 135 140 Arg Phe Phe Lys Glu Lys Leu Phe Asp Trp Thr Ser Asp Lys Pro Phe 145 150 155 160 Gly Thr Leu Tyr Val Leu Glu Leu Pro Lys Asp His Lys Gly Ile Tyr 165 170 175 Gly Lys Leu Thr Asp Ser Tyr Thr Tyr Met Arg Asn Gly Trp Asp Val 180 185 190 Gln Val Ser Ala Thr Ser Thr Gln Phe Asn Gly Gly Ser Leu Leu Val 195 200 205 Ala Met Val Pro Glu Leu Cys Ser Leu Lys Ala Arg Glu Glu Phe Gln 210 215 220 Leu Thr Leu Tyr Pro His Gln Phe Ile Asn Pro Arg Thr Asn Thr Thr 225 230 235 240 Ala His Ile Gln Val Pro Tyr Leu Gly Val Asn Arg His Asp Gln Gly 245 250 255 Lys Arg His Gln Ala Trp Ser Leu Val Val Met Val Leu Thr Pro Leu 260 265 270 Thr Thr Glu Ala Gln Met Asn Ser Gly Thr Val Glu Val Tyr Ala Asn 275 280 285 Ile Ala Pro Thr Asn Val Val Val Ala Gly Glu Leu Pro Gly Lys Gln 290 295 300 Gly Ile Ile Pro Val Ala Ala Ala Asp Gly Tyr Gly Gly Phe Gln Asn 305 310 315 320 Thr Asp Pro Lys Thr Ala Asp Pro Ile Tyr Gly His Val Tyr Asn Pro 325 330 335 Ser Arg Asn Asp Cys His Gly Arg Tyr Ser Asn Leu Met Asp Val Ala 340 345 350 Glu Ala Cys Pro Thr Leu Leu Asn Phe Asp Gly Lys Pro Tyr Val Val 355 360 365 Thr Lys Asn Asn Gly Asp Lys Val Met Ala Arg Phe Asp Val Ala Phe 370 375 380 Thr His Lys Val His Gly Asn Thr Phe Leu Ala Gly Leu Ala Asp Tyr 385 390 395 400 Tyr Thr Gln Tyr Ser Gly Ser Leu Asn Tyr His Phe Met Tyr Thr Gly 405 410 415 Pro Thr His His Lys Ala Lys Phe Met Val Ala Tyr Val Pro Pro Gly 420 425 430 Val Ala Val Asp Gln Leu Pro Ser Thr Pro Glu Asp Ala Ala His Cys 435 440 445 Tyr His Ala Glu Trp Asp Thr Gly Leu Asn Ser Ser Phe Ser Phe Ala 450 455 460 Val Pro Tyr Ile Ser Ala Ala Asp Phe Ser Tyr Thr His Thr Asp Thr 465 470 475 480 Pro Ala Met Ala Thr Thr Asn Gly Trp Val Val Val Leu Gln Val Thr 485 490 495 Asp Thr His Ser Ala Glu Ala Ala Val Val Val Ser Val Ser Ala Gly 500 505 510 Pro Asp Leu Glu Phe Arg Phe Pro Ile Asp Pro Val Arg Gln Thr Thr 515 520 525 Ser Ala Gly Glu Gly Ala Asp Val Val Thr Thr Asp Pro Ser Thr His 530 535 540 Gly Gly Asn Val Gln Glu Gly Arg Arg Lys His Thr Asp Val Ala Phe 545 550 555 560 Leu Leu Asp Arg Ser Thr His Val His Thr Asn Lys Thr Ser Phe Val 565 570 575 Val Asp Leu Met Asp Thr Arg Glu Lys Ala Leu Val Gly Ala Ile Leu 580 585 590 Arg Ala Ser Thr Tyr Tyr Phe Cys Asp Leu Glu Ile Ala Cys Val Gly 595 600 605 Asp His Thr Arg Val Phe Trp Gln Pro Asn Gly Ala Pro Arg Thr Thr 610 615 620 Gln Leu Gly Asp Asn Pro Met Val Phe Ala Lys Gly Gly Val Thr Arg 625 630 635 640 Phe Ala Ile Pro Phe Thr Ala Pro His Arg Leu Leu Ser Thr Val Tyr 645 650 655 Asn Gly Glu Cys Val Tyr Thr Lys Ala Ala Ala Ala Ile Arg Gly Asp 660 665 670 Arg Ala Ala Leu Ala Ala Lys Tyr Ala Asp Thr Asn His Thr Leu Pro 675 680 685 Pro Thr Phe Asn Phe Gly Tyr Val Thr Val Asp Lys Pro Val Asp Val 690 695 700 Tyr Tyr Arg Met Lys Arg Ala Glu Leu Tyr Cys Pro Arg Pro Leu Leu 705 710 715 720 Pro Ala Tyr Lys His Thr Asp Arg Asp Arg Phe Asp Ala Pro Ile Gly 725 730 735 Val Glu Arg Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp 740 745 750 Val Glu Ser Asn Pro Gly Pro 755 <210> 385 <211> 2277 <212> DNA <213> Artificial Sequence <220> <223> P1_SAT3_ZIM/05/91_cut_sites_removed <400> 385 ggagcgggcc agtcttcccc tgccactggg tcgcagaacc aatctggcaa cactggcagc 60 atcataaaca actactacat gcagcagtac caaaattcaa tggacaccca gcttggcgac 120 aacgccatct cgggtggttc aaatgaaggc agcacggaca ccacgtccac acacaccaac 180 aacacccaaa acaatgattg gttttcaaaa ttggcccaat ccgccatttc aggactcttc 240 ggggctctgt tggcggacaa aaagaccgag gaaacaacct tgcttgagga ccgaatcctc 300 acaacacgcc acaatactac cacatccacc acgcagagtt cggtgggagt gacttacggt 360 tatgcgtccg ctgatcggtt tctcccagga ccgaacacaa gtgggctgga aacgcgagtg 420 gaacaggccg agagattctt caaggagaga ctcttcactt ggaccgcttc gcagcagtac 480 gcccacgttc acctgcttga acttcccacc gaccacaagg gcatctacgg tgcaatggtg 540 gacaaccacg cttacatacg caacggatgg gacgtgcagg tctccgcgac cagcacgcag 600 ttcaatggtg gtacattgct cgtggcaatg gtcccagagt tacacgcgct cgacacacgt 660 agtgtttcac agctcacgct gtttccacac caattcatca acccacgcac aaacaccact 720 gcccacattg tcgttcccta cattggggta aacaggcatg accaggtaaa aatgcacaag 780 gcctggacat tggtggtcgc ggttctcgca ccgctcacaa catcaaacat gggacaggac 840 aacgttgagg tgtacgccaa catcgcacca accaacgttt ttgttgctgg cgagaagcct 900 accaaacaag gcatcttccc cgtggcctgc aacgacggct acggtggctt tcagaacact 960 gatcccaaga cctctgaccc catatacggg cttgtggcaa acccacctcg caccgctttt 1020 ccagggaggt tcaccaactt ccttgacgtg gcggaggcat gcccgacctt tctggacttc 1080 aacggaacac cttacgtcaa gaccagacac aacagtggga gcaagatact cacccacata 1140 gatttggctt ttgggcacaa gagtttcaag aacacttatc ttgctggtct cgcccagtat 1200 tacgcccaat acagcggatc tctcaacctg cacttcatgt acacgggtcc aacgcaatca 1260 aaggcacgct ttatggttgc atacgtacca ccagggacgg agcccgtacc atcaacaccc 1320 gaggaagcgg cgcactgcta ccactcggaa tgggacactg gactgaactc caagttcacg 1380 ttcacagtgc cgtacatgtc tgctgctgac tatgcctaca cctactgcga cgagcctgaa 1440 caggcctccg cacaagggtg ggtaacactg taccaaatta ctgacacaca tgaccctgat 1500 tcggctgtcc tcatctcagt tagcgcaggc gctgactttg aactcaggct gcccatcaac 1560 cccgttactc agacaacgag cgcgggggaa ggcgcagacg tggtgacaac ggacgttacc 1620 acacatggag gaacagttga cacaccaaga aggcagcaca ccaacgtgga gtttctgctg 1680 gacagattca cacacattgg gtcgatcacc gcctcaaaga ccattgacct ccttgagaca 1740 aaagaacaca cgctggtggg cgcactcctg cgttcagcta cgtactactt ttgtgacctt 1800 gaggtcgcgg tgcttggcaa cgcgaagtgg gttggatggg tgccgaatgg ctgcccacac 1860 accgaccgcg tggaagacaa cccagtcgtt cacgccaagg ggaatgttac ccgctttgca 1920 ctgccctaca cagccccgca cggagtgctt gccacgacct acaacgggac ttgcaagtat 1980 tccaagacgc aaagtgttaa accacgccga ggcgacatgg ctgtgctggc acaacgtgtt 2040 gaaggtgaac aacaaagatg caaacccacg acattcaact tcggtcgatt gttgtgtgat 2100 tcgggtgacg tgtactaccg aatgaagcgg gccgagcttt actgcccacg tccgctgatg 2160 gtcaggtaca ctcacaccac agacagatac aaagttgcgc ttgtgtcacc agctaagcaa 2220 ctgtgcaact tcgacctgtt aaagttggct ggagatgttg agtccaaccc tgggccc 2277 <210> 386 <211> 2277 <212> DNA <213> Foot-and-mouth disease virus <220> <221> CDS <222> (1)..(2277) <220> <221> misc_feature <222> (1)..(2277) <223> P1_SAT3_ZIM/05/91_wildtype <400> 386 gga gcg ggc cag tct tcc cct gcc aca ggg tcg cag aac caa tct ggc 48 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 aac act ggc agc atc ata aac aac tac tac atg cag cag tac cag aat 96 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 tca atg gac acc cag ctt ggc gac aac gcc atc tcg ggt ggt tca aat 144 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 gaa ggc agc acg gac acc acg tcc aca cac acc aac aac acc caa aac 192 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Asn Asn Thr Gln Asn 50 55 60 aat gat tgg ttt tca aaa ttg gcc caa tcc gcc att tca gga ctc ttc 240 Asn Asp Trp Phe Ser Lys Leu Ala Gln Ser Ala Ile Ser Gly Leu Phe 65 70 75 80 ggg gct ctg ttg gcg gac aaa aag acc gag gaa aca acc ttg ctc gag 288 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 gac cga atc ctc aca aca cgc cac aat act acc aca tcc acc acg cag 336 Asp Arg Ile Leu Thr Thr Arg His Asn Thr Thr Thr Ser Thr Thr Gln 100 105 110 agt tcg gtg gga gtg acg tac ggt tac gcg tcc gct gac cgg ttt ctc 384 Ser Ser Val Gly Val Thr Tyr Gly Tyr Ala Ser Ala Asp Arg Phe Leu 115 120 125 cca gga ccg aac aca agt ggg ctg gaa acg cga gtg gaa cag gcc gag 432 Pro Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Glu Gln Ala Glu 130 135 140 aga ttc ttc aag gag aga ctc ttc act tgg acc gct tcg cag cag tac 480 Arg Phe Phe Lys Glu Arg Leu Phe Thr Trp Thr Ala Ser Gln Gln Tyr 145 150 155 160 gcc cac gtt cac ctg ctt gaa ctt ccc acc gac cac aag ggc atc tac 528 Ala His Val His Leu Leu Glu Leu Pro Thr Asp His Lys Gly Ile Tyr 165 170 175 ggt gcc atg gtg gac aac cac gcg tac ata cgc aac gga tgg gac gtg 576 Gly Ala Met Val Asp Asn His Ala Tyr Ile Arg Asn Gly Trp Asp Val 180 185 190 cag gtc tcc gcg acc agc acg cag ttc aat ggt ggt aca ttg ctc gtg 624 Gln Val Ser Ala Thr Ser Thr Gln Phe Asn Gly Gly Thr Leu Leu Val 195 200 205 gca atg gtc cca gag tta cac gcg ctc gac acg cgt agt gtt tca cag 672 Ala Met Val Pro Glu Leu His Ala Leu Asp Thr Arg Ser Val Ser Gln 210 215 220 ctc acg ctg ttt cca cac caa ttc atc aac cca cgc aca aac acc act 720 Leu Thr Leu Phe Pro His Gln Phe Ile Asn Pro Arg Thr Asn Thr Thr 225 230 235 240 gcc cac att gtg gtt ccc tac att ggg gta aac agg cat gac cag gta 768 Ala His Ile Val Val Pro Tyr Ile Gly Val Asn Arg His Asp Gln Val 245 250 255 aaa atg cac aag gcc tgg aca ttg gtg gtc gcg gtt ctc gca ccg ctc 816 Lys Met His Lys Ala Trp Thr Leu Val Val Ala Val Leu Ala Pro Leu 260 265 270 acc aca tca aac atg gga cag gac aac gtt gag gtg tac gcc aac atc 864 Thr Thr Ser Asn Met Gly Gln Asp Asn Val Glu Val Tyr Ala Asn Ile 275 280 285 gca cca acc aac gtt ttt gtg gct ggc gag aag cct acc aaa caa ggc 912 Ala Pro Thr Asn Val Phe Val Ala Gly Glu Lys Pro Thr Lys Gln Gly 290 295 300 atc ttc ccc gtg gcc tgc aac gac ggc tac ggt ggc ttt cag aac act 960 Ile Phe Pro Val Ala Cys Asn Asp Gly Tyr Gly Gly Phe Gln Asn Thr 305 310 315 320 gat ccc aag acc tct gac ccc ata tac ggg ctg gtg gca aac cca cct 1008 Asp Pro Lys Thr Ser Asp Pro Ile Tyr Gly Leu Val Ala Asn Pro Pro 325 330 335 cgc acc gct ttt ccc ggg agg ttc acc aac ttc ctg gac gtg gcg gag 1056 Arg Thr Ala Phe Pro Gly Arg Phe Thr Asn Phe Leu Asp Val Ala Glu 340 345 350 gca tgc ccg acc ttt ctg gac ttc aac gga aca cct tac gtc aag acc 1104 Ala Cys Pro Thr Phe Leu Asp Phe Asn Gly Thr Pro Tyr Val Lys Thr 355 360 365 aga cac aac agt ggg agc aag ata ctc acc cac ata gat ttg gct ttt 1152 Arg His Asn Ser Gly Ser Lys Ile Leu Thr His Ile Asp Leu Ala Phe 370 375 380 ggg cac aag agt ttc aag aac act tat ctt gct ggt ctc gcc cag tat 1200 Gly His Lys Ser Phe Lys Asn Thr Tyr Leu Ala Gly Leu Ala Gln Tyr 385 390 395 400 tac gcc caa tac agt gga tct ctc aac ctg cac ttc atg tac acg ggt 1248 Tyr Ala Gln Tyr Ser Gly Ser Leu Asn Leu His Phe Met Tyr Thr Gly 405 410 415 ccc acg caa tca aag gca cgc ttt atg gtt gca tac gta cca ccc ggg 1296 Pro Thr Gln Ser Lys Ala Arg Phe Met Val Ala Tyr Val Pro Pro Gly 420 425 430 acg gag ccc gta cca tca aca ccc gag gaa gcg gcg cac tgc tac cac 1344 Thr Glu Pro Val Pro Ser Thr Pro Glu Glu Ala Ala His Cys Tyr His 435 440 445 tcg gaa tgg gac act gga ctg aac tcc aag ttc acg ttc aca gtg ccg 1392 Ser Glu Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Thr Val Pro 450 455 460 tac atg tct gca gct gac tat gcc tac acc tac tgc gac gag cct gaa 1440 Tyr Met Ser Ala Ala Asp Tyr Ala Tyr Thr Tyr Cys Asp Glu Pro Glu 465 470 475 480 cag gcc tcc gca caa ggg tgg gta aca ctg tac caa att act gac aca 1488 Gln Ala Ser Ala Gln Gly Trp Val Thr Leu Tyr Gln Ile Thr Asp Thr 485 490 495 cat gac cct gat tcg gct gtc ctc atc tca gtt agc gca ggc gct gac 1536 His Asp Pro Asp Ser Ala Val Leu Ile Ser Val Ser Ala Gly Ala Asp 500 505 510 ttt gag ctc agg ctg ccc atc aac ccc gtt act cag aca acg agc gcg 1584 Phe Glu Leu Arg Leu Pro Ile Asn Pro Val Thr Gln Thr Thr Ser Ala 515 520 525 ggg gaa ggc gca gac gtg gtg acc acg gac gtt acc acc cat gga gga 1632 Gly Glu Gly Ala Asp Val Val Thr Thr Asp Val Thr Thr His Gly Gly 530 535 540 aca gtt gac aca cca aga agg cag cac acc aac gtg gag ttt ctg ctg 1680 Thr Val Asp Thr Pro Arg Arg Gln His Thr Asn Val Glu Phe Leu Leu 545 550 555 560 gac aga ttc aca cac att ggg tcg atc acc gcc tca aag acc att gac 1728 Asp Arg Phe Thr His Ile Gly Ser Ile Thr Ala Ser Lys Thr Ile Asp 565 570 575 ctc ctc gag aca aaa gaa cac acg ctg gtg ggc gca ctc ctg cgt tca 1776 Leu Leu Glu Thr Lys Glu His Thr Leu Val Gly Ala Leu Leu Arg Ser 580 585 590 gct acg tac tac ttt tgt gac ctc gag gtc gcg gtg ctt ggc aac gcg 1824 Ala Thr Tyr Tyr Phe Cys Asp Leu Glu Val Ala Val Leu Gly Asn Ala 595 600 605 aag tgg gtt gga tgg gtg ccg aat ggc tgc cca cac acc gac cgc gtg 1872 Lys Trp Val Gly Trp Val Pro Asn Gly Cys Pro His Thr Asp Arg Val 610 615 620 gaa gac aac cca gtc gtt cac gcc aag ggg aat gtt acc cgc ttt gca 1920 Glu Asp Asn Pro Val Val His Ala Lys Gly Asn Val Thr Arg Phe Ala 625 630 635 640 ctg ccc tac aca gcc ccg cac gga gtg ctt gcc acg acc tac aac ggg 1968 Leu Pro Tyr Thr Ala Pro His Gly Val Leu Ala Thr Thr Tyr Asn Gly 645 650 655 act tgc aag tat tcc aag acg caa agt gtt aaa cca cgc cgt ggc gac 2016 Thr Cys Lys Tyr Ser Lys Thr Gln Ser Val Lys Pro Arg Arg Gly Asp 660 665 670 atg gct gtg ctg gca caa cgc gtt gaa ggt gaa caa caa aga tgc aaa 2064 Met Ala Val Leu Ala Gln Arg Val Glu Gly Glu Gln Gln Arg Cys Lys 675 680 685 ccc acg aca ttc aac ttc ggt cga ttg ttg tgt gat tcg ggt gac gtg 2112 Pro Thr Thr Phe Asn Phe Gly Arg Leu Leu Cys Asp Ser Gly Asp Val 690 695 700 tac tac cga atg aag cgg gcc gag ctt tac tgc cca cgt ccg ctg atg 2160 Tyr Tyr Arg Met Lys Arg Ala Glu Leu Tyr Cys Pro Arg Pro Leu Met 705 710 715 720 gtc agg tac act cac acc aca gac aga tac aaa gtt gcg ctt gtg tca 2208 Val Arg Tyr Thr His Thr Thr Asp Arg Tyr Lys Val Ala Leu Val Ser 725 730 735 cca gct aag caa ctg tgc aac ttc gac ctg tta aag ttg gct gga gat 2256 Pro Ala Lys Gln Leu Cys Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp 740 745 750 gtt gag tcc aac cct ggg ccc 2277 Val Glu Ser Asn Pro Gly Pro 755 <210> 387 <211> 759 <212> PRT <213> Foot-and-mouth disease virus <400> 387 Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 1 5 10 15 Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 20 25 30 Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 35 40 45 Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Asn Asn Thr Gln Asn 50 55 60 Asn Asp Trp Phe Ser Lys Leu Ala Gln Ser Ala Ile Ser Gly Leu Phe 65 70 75 80 Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 85 90 95 Asp Arg Ile Leu Thr Thr Arg His Asn Thr Thr Thr Ser Thr Thr Gln 100 105 110 Ser Ser Val Gly Val Thr Tyr Gly Tyr Ala Ser Ala Asp Arg Phe Leu 115 120 125 Pro Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Glu Gln Ala Glu 130 135 140 Arg Phe Phe Lys Glu Arg Leu Phe Thr Trp Thr Ala Ser Gln Gln Tyr 145 150 155 160 Ala His Val His Leu Leu Glu Leu Pro Thr Asp His Lys Gly Ile Tyr 165 170 175 Gly Ala Met Val Asp Asn His Ala Tyr Ile Arg Asn Gly Trp Asp Val 180 185 190 Gln Val Ser Ala Thr Ser Thr Gln Phe Asn Gly Gly Thr Leu Leu Val 195 200 205 Ala Met Val Pro Glu Leu His Ala Leu Asp Thr Arg Ser Val Ser Gln 210 215 220 Leu Thr Leu Phe Pro His Gln Phe Ile Asn Pro Arg Thr Asn Thr Thr 225 230 235 240 Ala His Ile Val Val Pro Tyr Ile Gly Val Asn Arg His Asp Gln Val 245 250 255 Lys Met His Lys Ala Trp Thr Leu Val Val Ala Val Leu Ala Pro Leu 260 265 270 Thr Thr Ser Asn Met Gly Gln Asp Asn Val Glu Val Tyr Ala Asn Ile 275 280 285 Ala Pro Thr Asn Val Phe Val Ala Gly Glu Lys Pro Thr Lys Gln Gly 290 295 300 Ile Phe Pro Val Ala Cys Asn Asp Gly Tyr Gly Gly Phe Gln Asn Thr 305 310 315 320 Asp Pro Lys Thr Ser Asp Pro Ile Tyr Gly Leu Val Ala Asn Pro Pro 325 330 335 Arg Thr Ala Phe Pro Gly Arg Phe Thr Asn Phe Leu Asp Val Ala Glu 340 345 350 Ala Cys Pro Thr Phe Leu Asp Phe Asn Gly Thr Pro Tyr Val Lys Thr 355 360 365 Arg His Asn Ser Gly Ser Lys Ile Leu Thr His Ile Asp Leu Ala Phe 370 375 380 Gly His Lys Ser Phe Lys Asn Thr Tyr Leu Ala Gly Leu Ala Gln Tyr 385 390 395 400 Tyr Ala Gln Tyr Ser Gly Ser Leu Asn Leu His Phe Met Tyr Thr Gly 405 410 415 Pro Thr Gln Ser Lys Ala Arg Phe Met Val Ala Tyr Val Pro Pro Gly 420 425 430 Thr Glu Pro Val Pro Ser Thr Pro Glu Glu Ala Ala His Cys Tyr His 435 440 445 Ser Glu Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Thr Val Pro 450 455 460 Tyr Met Ser Ala Ala Asp Tyr Ala Tyr Thr Tyr Cys Asp Glu Pro Glu 465 470 475 480 Gln Ala Ser Ala Gln Gly Trp Val Thr Leu Tyr Gln Ile Thr Asp Thr 485 490 495 His Asp Pro Asp Ser Ala Val Leu Ile Ser Val Ser Ala Gly Ala Asp 500 505 510 Phe Glu Leu Arg Leu Pro Ile Asn Pro Val Thr Gln Thr Thr Ser Ala 515 520 525 Gly Glu Gly Ala Asp Val Val Thr Thr Asp Val Thr Thr His Gly Gly 530 535 540 Thr Val Asp Thr Pro Arg Arg Gln His Thr Asn Val Glu Phe Leu Leu 545 550 555 560 Asp Arg Phe Thr His Ile Gly Ser Ile Thr Ala Ser Lys Thr Ile Asp 565 570 575 Leu Leu Glu Thr Lys Glu His Thr Leu Val Gly Ala Leu Leu Arg Ser 580 585 590 Ala Thr Tyr Tyr Phe Cys Asp Leu Glu Val Ala Val Leu Gly Asn Ala 595 600 605 Lys Trp Val Gly Trp Val Pro Asn Gly Cys Pro His Thr Asp Arg Val 610 615 620 Glu Asp Asn Pro Val Val His Ala Lys Gly Asn Val Thr Arg Phe Ala 625 630 635 640 Leu Pro Tyr Thr Ala Pro His Gly Val Leu Ala Thr Thr Tyr Asn Gly 645 650 655 Thr Cys Lys Tyr Ser Lys Thr Gln Ser Val Lys Pro Arg Arg Gly Asp 660 665 670 Met Ala Val Leu Ala Gln Arg Val Glu Gly Glu Gln Gln Arg Cys Lys 675 680 685 Pro Thr Thr Phe Asn Phe Gly Arg Leu Leu Cys Asp Ser Gly Asp Val 690 695 700 Tyr Tyr Arg Met Lys Arg Ala Glu Leu Tyr Cys Pro Arg Pro Leu Met 705 710 715 720 Val Arg Tyr Thr His Thr Thr Asp Arg Tyr Lys Val Ala Leu Val Ser 725 730 735 Pro Ala Lys Gln Leu Cys Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp 740 745 750 Val Glu Ser Asn Pro Gly Pro 755 <210> 388 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(C163A)-GLuc(-1M) <400> 388 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcatccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcacg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacg ttgggagact gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctacgctg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 389 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(D123P)-GLuc(-1M) <400> 389 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcatccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcacg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgcccccg ttgggagact gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 390 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(G125P)-GLuc(-1M) <400> 390 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcatccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcacg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacg ttccgagact gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 391 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(I128P)-GLuc(-1M) <400> 391 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcatccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcacg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacg ttgggagact gcctttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 392 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(I22P)-GLuc(-1M) <400> 392 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcccccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcacg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacg ttgggagact gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 393 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(I22P/L127P)-GLuc(-1M) <400> 393 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcccccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcacg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacg ttgggagacc gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 394 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(I22P/T100P/L127P)-GLuc(-1M) <400> 394 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcccccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcccg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacg ttgggagacc gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 395 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(I22P/T100P/V124P)-GLuc(-1M) <400> 395 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcccccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcccg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacc ctgggagact gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 396 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(I99P)-GLuc(-1M) <400> 396 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcatccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agaccccacg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacg ttgggagact gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 397 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(L127P)-GLuc(-1M) <400> 397 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcatccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcacg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacg ttgggagacc gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 398 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(L23P)-GLuc(-1M) <400> 398 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcatccctg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcacg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacg ttgggagact gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 399 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(R126P)-GLuc(-1M) <400> 399 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcatccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcacg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacg ttgggccact gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 400 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(T100P)-GLuc(-1M) <400> 400 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcatccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcccg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacg ttgggagact gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 401 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(T100P/L127P)-GLuc(-1M) <400> 401 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcatccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcccg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacg ttgggagacc gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 402 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(T34A/M88L)-GLuc(-1M) <400> 402 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcatccttg acggtaagac ggtggccatc tgctgcgccg ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct cctggtgctc caccgtggca accgtgtgag 3600 agacatcacg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacg ttgggagact gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 403 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(V124P)-GLuc(-1M) <400> 403 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcatccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcacg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacc ctgggagact gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 404 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(V124P/L127P)-GLuc(-1M) <400> 404 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcatccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcacg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacc ctgggagacc gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 405 <211> 6674 <212> DNA <213> Artificial Sequence <220> <223> pJJP_O1P1-3C(WT)-GLuc(-1M) <400> 405 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaagat taaacttaag 840 tgactctatt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 900 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 960 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 1020 cagaatcgat attggatccg ccgccaccat gggagccggg caatccagcc cggcaaccgg 1080 gtcacagaac caatcaggca acactgggag catcatcaac aattactaca tgcagcagta 1140 ccaaaactct atggacacac aacttggtga caacgctaca agcggaggct caaacgaggg 1200 gtccacggac acaacctcca cccacacaac caacactcag aacaacgact ggttctcgaa 1260 gctggctagc tccgctttca gcggtctttt cggcgctctt ctcgccgaca agaaaaccga 1320 ggagaccact cttcttgagg accgcatcct cactactcgt aacggacaca ccacctcgac 1380 aacccagtcg agcgtaggag tcacatacgg gtatgcaacg gctgaggatt tcgtgagcgg 1440 gccaaacacc tctggtcttg agaccagggt tgcccaggca gagcggttct ttaaaaccca 1500 cctgttcgac tgggtcacaa gtgacccgtt cggacggtgc cacctgctag aacttccaac 1560 tgaccacaaa ggtgtctatg gcagcctgac cgactcgtat gcttatatga ggaacggctg 1620 ggatgttgaa gtcactgctg tgggaaatca gttcaatgga ggatgcctgt tggtggctat 1680 ggtgccagaa ctttgctcca tacagaagag ggagctgtac cagctcacgc tctttcctca 1740 ccagttcatc aaccctcgga cgaacatgac agcacacatc actgtgccct ttgttggcgt 1800 caaccgttat gaccagtaca aggtacacaa accttggacc ctcgtggtta tggttgtagc 1860 ccccctgacc gtcaacagtg aaggtgcccc gcaaatcaag gtgtatgcca acatcgcacc 1920 taccaacgta cacgtcgcgg gtgaattccc ttccaaagag gggatcttcc ctgtggcttg 1980 cagcgatggt tatggcggtc tggtgacaac tgacccgaaa acggctgacc ccgcttacgg 2040 gaaagtgttt aacccccccc gcaacatgtt gccggggcgg ttcaccaatt ttcttgacgt 2100 ggctgaggcg tgccccacgt ttctccactt cgagggtgac gtgccatacg tgaccacgaa 2160 gacggattca gacagggtgc tcgctcagtt cgacttgtct ttggcagcaa agcacatgtc 2220 caacaccttc cttgcaggtc tcgcccagta ctacacacag tacagcggca ccatcaacct 2280 gcacttcatg ttcacagggc ctactgacgc gaaggcgcgt tacatgattg cgtatgctcc 2340 tcctggcatg gaaccaccta aaacgccaga ggcggctgcc cactgcatcc atgctgaatg 2400 ggacacaggg ttgaactcaa aattcacatt ttcaatccct tacctttcgg cggctgatta 2460 cgcttacaca gcgtctgaca ctgctgagac cacaaatgta cagggatggg tttgcctgtt 2520 tcaaataaca cacgggaaag ctgacggcga cgcactggtc gttttggcca gcgccggaaa 2580 ggactttgag ctgcgcctgc cggtggatgc taggacccag actacctccg cgggcgagtc 2640 agcagacccc gtgaccgcca ccgttgagaa ttacggtggc gagacacagg tccagaggcg 2700 ccaacacacg gacgtgtcat ttatattaga cagatttgtg aaagtgacac caaaagacca 2760 aattaatgta ttggacctga tgcaaacccc tgctcacact ttggtgggag cactccttcg 2820 tactgccact tactatttcg ctgacttaga ggtggcagtg aagcacgagg gaaacctcac 2880 ctgggtgccg aacggggcgc ctgaagcggc gttggacaac accaccaacc caacagctta 2940 ccacaaggca ccactcaccc gacttgcact gccttacacg gcgccacacc gcgtgttggc 3000 tactgtttac aacgggaaca gcaagtatgg tgacggcacg gtggccaatg tgagaggtga 3060 tctgcaagtg ttggcccaga aggcggcgag agcgctgcct acctccttca actacggtgc 3120 cattaaagct actcgggtga ctgaactgct ttaccgcatg aagagggctg agacatactg 3180 tccccggcct cttttggcca ttcacccgga ccaggctaga cacaagcaga agattgtggc 3240 tccggtgaaa cagcttctaa attttgacct gctcaaattg gcgggagatg tggagtccaa 3300 ccctgggccc agtggtgccc caccgaccga cttgcaaaag atggtcatga gcaacactaa 3360 gcctgttgag ctcatccttg acggtaagac ggtggccatc tgctgcgcca ccggagtgtt 3420 tggtactgcc tacctcgtgc ctcgtcacct tttcgcagaa aagtacgaca ggatcatgtt 3480 ggacggcagg gccatgacag acagtgacta cagagtgttt gagtttgaga ttaaagtaaa 3540 aggacaggac atgctctcag acgctgcgct catggtgctc caccgtggca accgtgtgag 3600 agacatcacg aaacactttc gtgatacagc aagaatgaag aaaggtaccc ccgttgtcgg 3660 cgtgatcaac aacgccgacg ttgggagact gattttctcc ggtgaggccc tcacctacaa 3720 ggacattgta gtgtgcatgg atggagacac catgccgggc ctatttgcct acagagccgc 3780 taccaaggct ggctactgtg gaggagccgt tcttgccaag gacggagctg acacatttat 3840 cgtcggcact cactccgcag gaggcaatgg agtcgggtac tgctcatgcg tatctaggtc 3900 catgctcttg aagatgaagg cacacattga ccccgaacca caccacgagg aattccacaa 3960 gcaaaagatc attgcaccag caaagcagct tctgaatttt gacctgctca agttggccgg 4020 agacgttgag tccaacccag gaccaggagt caaagttctg tttgccctga tctgcatcgc 4080 tgtggccgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag 4140 caacttcgcg accacggatc tcgatgctga ccgagggaag ttgcccggca agaagctgcc 4200 gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa gctggctgca ccaggggctg 4260 tctgatctgc ctgtcccaca tcaaatgcac gcccaagatg aagaagttca tcccaggacg 4320 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt 4380 tgacattcct gagattcctg ggttcaagga cttggagcca atggagcagt tcatcgcaca 4440 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg 4500 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca 4560 gggccaggtg gacaagatca agggggccgg tggtgactaa tctagagata tcagacatga 4620 taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta 4680 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 4740 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 4800 tttaaagcaa gtaaaacctc tacaaatgtg gtaaatccga taaggatcgt cgactggctg 4860 cagtcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4920 gtatccgctc tcgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4980 tatgagtatt caacatttca gagtggccct tattcccttt tttgcagcat tttgccttcc 5040 tgtttttgct cacccagaaa cactggtgaa agtaaaagat gctgaagacc agttgggtgc 5100 cagagtgggt tacattgaac tggacctcaa cagtggtaaa atccttgaga gttttagacc 5160 tgaagaaaga tttccaatga tgagcacttt taaagtactg ctatgtggtg ctgtattatc 5220 cagaattgat gcagggcaag agcaacttgg tagaagaata cactattctc agaatgactt 5280 ggttgagtac tcaccagtca cagaaaagca tcttacagat ggcatgacag taagagaatt 5340 atgcagtgct gccataacca tgagtgataa cacagctgcc aacttacttc tgacaaccat 5400 tggtggacct aaagagctaa ctgctttttt gcacaacatg ggggaccatg taactagact 5460 tgataggtgg gaacctgagc tgaatgaagc cattccaaat gatgagaggg acaccacaat 5520 gcctgtagca atggcaacaa ctttgaggaa actattaact ggtgaactac ttactctagc 5580 ttccagacaa caattaatag actggatgga ggcagataaa gttgcaggac cacttctgag 5640 gtcagcctta cctgctggct ggtttattgc tgataaatct ggagcaggtg agagagggtc 5700 taggggtatc attgcagcac tggggccaga tggtaagccc tccagaattg tagttatcta 5760 cacaacaggg agtcaggcaa ctatggatga aagaaataga cagatagctg agataggtgc 5820 ctcactgatt aagcattggt gagcatgcga ccaagtttac tcatatatac tttagattga 5880 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 5940 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg gttaaccccg tagaaaagat 6000 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 6060 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 6120 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 6180 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 6240 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 6300 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 6360 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 6420 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 6480 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 6540 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 6600 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ccactgcaat 6660 ggccggccag atct 6674 <210> 406 <211> 6531 <212> DNA <213> Artificial Sequence <220> <223> pSNAP_3C(L23P) <400> 406 aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 60 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 120 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 180 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 240 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 300 ccttgagagt tttcgccccg aagaacgttt cccaatgatg agcactttta aagttctgct 360 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca 420 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 480 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 540 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 600 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 660 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 720 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 780 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 840 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 900 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 960 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 1020 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag 1080 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat 1140 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 1200 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 1260 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 1320 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 1380 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 1440 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 1500 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag 1560 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 1620 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 1680 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 1740 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 1800 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 1860 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 1920 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 1980 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 2040 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 2100 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 2160 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 2220 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 2280 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 2340 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 2400 cgagcgcagc gagtcagtga gcgaggaagc tatggtgcac tctcagtaca atctgctctg 2460 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc 2520 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 2580 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 2640 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc 2700 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt 2760 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc 2820 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat 2880 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa 2940 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcc 3000 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt 3060 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat 3120 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc 3180 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt 3240 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa 3300 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat 3360 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 3420 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt 3480 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca 3540 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg 3600 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atgtccgcac 3660 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg 3720 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac 3780 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga 3840 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta 3900 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt 3960 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg 4020 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt 4080 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt 4140 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag 4200 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc 4260 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca 4320 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt 4380 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg 4440 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac 4500 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac 4560 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg 4620 aatggtgcat gccggcatgc cgccctttcg tcttcaagaa ttaattccca attccccagg 4680 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 4740 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag 4800 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattcccc 4860 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 4920 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg 4980 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattaattc 5040 cccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 5100 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt 5160 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggaattaa 5220 ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 5280 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 5340 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat 5400 taattcccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 5460 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg 5520 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg 5580 aattggggat cggaattaat tcccggttta aaccggggat ctcgatcccg cgaaattaat 5640 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt 5700 taactttaag aaggagatat acatatgagt ggtgccccac cgaccgactt gcaaaagatg 5760 gtcatgagca acactaagcc tgttgagctc atccctgacg gtaagacggt ggccatctgc 5820 tgcgccaccg gagtgtttgg tactgcctac ctcgtgcctc gtcacctttt cgcagaaaag 5880 tacgacagga tcatgttgga cggcagggcc atgacagaca gtgactacag agtgtttgag 5940 tttgagatta aagtaaaagg acaggacatg ctctcagacg ctgcgctcat ggtgctccac 6000 cgtggcaacc gtgtgagaga catcacgaaa cactttcgtg atacagcaag aatgaagaaa 6060 ggtacccccg ttgtcggcgt gatcaacaac gccgacgttg ggagactgat tttctccggt 6120 gaggccctca cctacaagga cattgtagtg tgcatggatg gagacaccat gccgggccta 6180 tttgcctaca gagccgctac caaggctggc tactgtggag gagccgttct tgccaaggac 6240 ggagctgaca catttatcgt cggcactcac tccgcaggag gcaatggagt cgggtactgc 6300 tcatgcgtat ctaggtccat gctcttgaag atgaaggcac acattgaccc cgaaccacac 6360 cacgagtagc tcgaggttaa ttaagcggcc gcattgatcc ggctgctaac aaagcccgaa 6420 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct 6480 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t 6531 <210> 407 <211> 6531 <212> DNA <213> Artificial Sequence <220> <223> pSNAP_3C(L42H) <400> 407 aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 60 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 120 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 180 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 240 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 300 ccttgagagt tttcgccccg aagaacgttt cccaatgatg agcactttta aagttctgct 360 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca 420 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 480 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 540 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 600 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 660 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 720 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 780 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 840 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 900 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 960 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 1020 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag 1080 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat 1140 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 1200 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 1260 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 1320 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 1380 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 1440 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 1500 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag 1560 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 1620 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 1680 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 1740 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 1800 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 1860 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 1920 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 1980 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 2040 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 2100 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 2160 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 2220 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 2280 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 2340 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 2400 cgagcgcagc gagtcagtga gcgaggaagc tatggtgcac tctcagtaca atctgctctg 2460 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc 2520 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 2580 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 2640 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc 2700 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt 2760 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc 2820 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat 2880 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa 2940 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcc 3000 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt 3060 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat 3120 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc 3180 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt 3240 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa 3300 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat 3360 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 3420 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt 3480 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca 3540 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg 3600 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atgtccgcac 3660 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg 3720 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac 3780 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga 3840 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta 3900 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt 3960 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg 4020 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt 4080 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt 4140 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag 4200 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc 4260 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca 4320 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt 4380 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg 4440 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac 4500 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac 4560 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg 4620 aatggtgcat gccggcatgc cgccctttcg tcttcaagaa ttaattccca attccccagg 4680 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 4740 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag 4800 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattcccc 4860 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 4920 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg 4980 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattaattc 5040 cccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 5100 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt 5160 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggaattaa 5220 ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 5280 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 5340 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat 5400 taattcccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 5460 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg 5520 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg 5580 aattggggat cggaattaat tcccggttta aaccggggat ctcgatcccg cgaaattaat 5640 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt 5700 taactttaag aaggagatat acatatgagt ggtgccccac cgaccgactt gcaaaagatg 5760 gtcatgagca acactaagcc tgttgagctc atccttgacg gtaagacggt ggccatctgc 5820 tgcgccaccg gagtgtttgg tactgcctac cacgtgcctc gtcacctttt cgcagaaaag 5880 tacgacagga tcatgttgga cggcagggcc atgacagaca gtgactacag agtgtttgag 5940 tttgagatta aagtaaaagg acaggacatg ctctcagacg ctgcgctcat ggtgctccac 6000 cgtggcaacc gtgtgagaga catcacgaaa cactttcgtg atacagcaag aatgaagaaa 6060 ggtacccccg ttgtcggcgt gatcaacaac gccgacgttg ggagactgat tttctccggt 6120 gaggccctca cctacaagga cattgtagtg tgcatggatg gagacaccat gccgggccta 6180 tttgcctaca gagccgctac caaggctggc tactgtggag gagccgttct tgccaaggac 6240 ggagctgaca catttatcgt cggcactcac tccgcaggag gcaatggagt cgggtactgc 6300 tcatgcgtat ctaggtccat gctcttgaag atgaaggcac acattgaccc cgaaccacac 6360 cacgagtagc tcgaggttaa ttaagcggcc gcattgatcc ggctgctaac aaagcccgaa 6420 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct 6480 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t 6531 <210> 408 <211> 6531 <212> DNA <213> Artificial Sequence <220> <223> pSNAP_3C(T100P) <400> 408 aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 60 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 120 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 180 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 240 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 300 ccttgagagt tttcgccccg aagaacgttt cccaatgatg agcactttta aagttctgct 360 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca 420 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 480 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 540 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 600 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 660 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 720 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 780 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 840 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 900 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 960 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 1020 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag 1080 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat 1140 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 1200 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 1260 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 1320 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 1380 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 1440 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 1500 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag 1560 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 1620 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 1680 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 1740 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 1800 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 1860 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 1920 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 1980 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 2040 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 2100 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 2160 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 2220 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 2280 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 2340 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 2400 cgagcgcagc gagtcagtga gcgaggaagc tatggtgcac tctcagtaca atctgctctg 2460 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc 2520 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 2580 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 2640 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc 2700 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt 2760 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc 2820 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat 2880 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa 2940 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcc 3000 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt 3060 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat 3120 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc 3180 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt 3240 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa 3300 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat 3360 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 3420 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt 3480 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca 3540 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg 3600 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atgtccgcac 3660 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg 3720 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac 3780 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga 3840 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta 3900 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt 3960 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg 4020 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt 4080 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt 4140 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag 4200 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc 4260 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca 4320 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt 4380 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg 4440 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac 4500 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac 4560 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg 4620 aatggtgcat gccggcatgc cgccctttcg tcttcaagaa ttaattccca attccccagg 4680 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 4740 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag 4800 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattcccc 4860 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 4920 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg 4980 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattaattc 5040 cccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 5100 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt 5160 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggaattaa 5220 ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 5280 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 5340 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat 5400 taattcccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 5460 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg 5520 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg 5580 aattggggat cggaattaat tcccggttta aaccggggat ctcgatcccg cgaaattaat 5640 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt 5700 taactttaag aaggagatat acatatgagt ggtgccccac cgaccgactt gcaaaagatg 5760 gtcatgagca acactaagcc tgttgagctc atccttgacg gtaagacggt ggccatctgc 5820 tgcgccaccg gagtgtttgg tactgcctac ctcgtgcctc gtcacctttt cgcagaaaag 5880 tacgacagga tcatgttgga cggcagggcc atgacagaca gtgactacag agtgtttgag 5940 tttgagatta aagtaaaagg acaggacatg ctctcagacg ctgcgctcat ggtgctccac 6000 cgtggcaacc gtgtgagaga catcccgaaa cactttcgtg atacagcaag aatgaagaaa 6060 ggtacccccg ttgtcggcgt gatcaacaac gccgacgttg ggagactgat tttctccggt 6120 gaggccctca cctacaagga cattgtagtg tgcatggatg gagacaccat gccgggccta 6180 tttgcctaca gagccgctac caaggctggc tactgtggag gagccgttct tgccaaggac 6240 ggagctgaca catttatcgt cggcactcac tccgcaggag gcaatggagt cgggtactgc 6300 tcatgcgtat ctaggtccat gctcttgaag atgaaggcac acattgaccc cgaaccacac 6360 cacgagtagc tcgaggttaa ttaagcggcc gcattgatcc ggctgctaac aaagcccgaa 6420 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct 6480 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t 6531 <210> 409 <211> 6531 <212> DNA <213> Artificial Sequence <220> <223> pSNAP_3C(WT) <400> 409 aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 60 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 120 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 180 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 240 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 300 ccttgagagt tttcgccccg aagaacgttt cccaatgatg agcactttta aagttctgct 360 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca 420 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 480 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 540 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 600 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 660 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 720 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 780 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 840 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 900 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 960 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 1020 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag 1080 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat 1140 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 1200 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 1260 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 1320 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 1380 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 1440 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 1500 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag 1560 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 1620 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 1680 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 1740 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 1800 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 1860 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 1920 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 1980 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 2040 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 2100 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 2160 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 2220 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 2280 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 2340 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 2400 cgagcgcagc gagtcagtga gcgaggaagc tatggtgcac tctcagtaca atctgctctg 2460 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc 2520 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 2580 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 2640 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc 2700 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt 2760 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc 2820 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat 2880 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa 2940 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcc 3000 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt 3060 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat 3120 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc 3180 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt 3240 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa 3300 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat 3360 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 3420 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt 3480 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca 3540 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg 3600 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atgtccgcac 3660 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg 3720 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac 3780 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga 3840 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta 3900 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt 3960 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg 4020 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt 4080 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt 4140 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag 4200 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc 4260 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca 4320 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt 4380 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg 4440 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac 4500 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac 4560 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg 4620 aatggtgcat gccggcatgc cgccctttcg tcttcaagaa ttaattccca attccccagg 4680 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 4740 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag 4800 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattcccc 4860 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 4920 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg 4980 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattaattc 5040 cccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 5100 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt 5160 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggaattaa 5220 ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 5280 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 5340 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat 5400 taattcccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 5460 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg 5520 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg 5580 aattggggat cggaattaat tcccggttta aaccggggat ctcgatcccg cgaaattaat 5640 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt 5700 taactttaag aaggagatat acatatgagt ggtgccccac cgaccgactt gcaaaagatg 5760 gtcatgagca acactaagcc tgttgagctc atccttgacg gtaagacggt ggccatctgc 5820 tgcgccaccg gagtgtttgg tactgcctac ctcgtgcctc gtcacctttt cgcagaaaag 5880 tacgacagga tcatgttgga cggcagggcc atgacagaca gtgactacag agtgtttgag 5940 tttgagatta aagtaaaagg acaggacatg ctctcagacg ctgcgctcat ggtgctccac 6000 cgtggcaacc gtgtgagaga catcacgaaa cactttcgtg atacagcaag aatgaagaaa 6060 ggtacccccg ttgtcggcgt gatcaacaac gccgacgttg ggagactgat tttctccggt 6120 gaggccctca cctacaagga cattgtagtg tgcatggatg gagacaccat gccgggccta 6180 tttgcctaca gagccgctac caaggctggc tactgtggag gagccgttct tgccaaggac 6240 ggagctgaca catttatcgt cggcactcac tccgcaggag gcaatggagt cgggtactgc 6300 tcatgcgtat ctaggtccat gctcttgaag atgaaggcac acattgaccc cgaaccacac 6360 cacgagtagc tcgaggttaa ttaagcggcc gcattgatcc ggctgctaac aaagcccgaa 6420 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct 6480 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t 6531 <210> 410 <211> 6590 <212> DNA <213> Artificial Sequence <220> <223> pSNAP_Flag-3C(L127P) <400> 410 aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 60 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 120 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 180 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 240 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 300 ccttgagagt tttcgccccg aagaacgttt cccaatgatg agcactttta aagttctgct 360 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca 420 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 480 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 540 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 600 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 660 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 720 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 780 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 840 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 900 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 960 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 1020 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag 1080 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat 1140 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 1200 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 1260 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 1320 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 1380 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 1440 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 1500 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag 1560 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 1620 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 1680 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 1740 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 1800 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 1860 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 1920 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 1980 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 2040 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 2100 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 2160 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 2220 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 2280 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 2340 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 2400 cgagcgcagc gagtcagtga gcgaggaagc tatggtgcac tctcagtaca atctgctctg 2460 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc 2520 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 2580 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 2640 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc 2700 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt 2760 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc 2820 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat 2880 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa 2940 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcc 3000 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt 3060 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat 3120 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc 3180 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt 3240 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa 3300 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat 3360 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 3420 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt 3480 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca 3540 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg 3600 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atgtccgcac 3660 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg 3720 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac 3780 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga 3840 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta 3900 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt 3960 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg 4020 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt 4080 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt 4140 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag 4200 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc 4260 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca 4320 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt 4380 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg 4440 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac 4500 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac 4560 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg 4620 aatggtgcat gccggcatgc cgccctttcg tcttcaagaa ttaattccca attccccagg 4680 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 4740 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag 4800 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattcccc 4860 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 4920 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg 4980 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattaattc 5040 cccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 5100 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt 5160 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggaattaa 5220 ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 5280 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 5340 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat 5400 taattcccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 5460 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg 5520 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg 5580 aattggggat cggaattaat tcccggttta aaccggggat ctcgatcccg cgaaattaat 5640 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt 5700 taactttaag aaggagatat acatatggac tataaagacg acgacgacaa acccgggagt 5760 ggtgccccac cgaccgactt gcaaaagatg gtcatgagca acactaagcc tgttgagctc 5820 atccttgacg gtaagacggt ggccatctgc tgcgccaccg gagtgtttgg tactgcctac 5880 ctcgtgcctc gtcacctttt cgcagaaaag tacgacagga tcatgttgga cggcagggcc 5940 atgacagaca gtgactacag agtgtttgag tttgagatta aagtaaaagg acaggacatg 6000 ctctcagacg ctgcgctcat ggtgctccac cgtggcaacc gtgtgagaga catcacgaaa 6060 cactttcgtg atacagcaag aatgaagaaa ggtacccccg ttgtcggcgt gatcaacaac 6120 gccgacgttg ggagaccgat tttctccggt gaggccctca cctacaagga cattgtagtg 6180 tgcatggatg gagacaccat gccgggccta tttgcctaca gagccgctac caaggctggc 6240 tactgtggag gagccgttct tgccaaggac ggagctgaca catttatcgt cggcactcac 6300 tccgcaggag gcaatggagt cgggtactgc tcatgcgtat ctaggtccat gctcttgaag 6360 atgaaggcac acattgaccc cgaaccacac cacgagtagt cgacagacaa gcttaggtat 6420 ttattcggct cgaggttaat taagcggccg cattgatccg gctgctaaca aagcccgaaa 6480 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc 6540 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat 6590 <210> 411 <211> 627 <212> DNA <213> Bovine rhinitis <220> <221> CDS <222> (1)..(627) <220> <221> misc_feature <222> (1)..(627) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(627) <223> Bovine_Rhinitis_A_Sd-1_KP236128_3C(C23P) <400> 411 agt ggt gct cca cca aca gac atg cag caa cac gtt ctc agg aac gtg 48 Ser Gly Ala Pro Pro Thr Asp Met Gln Gln His Val Leu Arg Asn Val 1 5 10 15 cgc cca ata tca ctt gtt ccn gat gga aag gtt gtg tct atg tgc tgt 96 Arg Pro Ile Ser Leu Val Pro Asp Gly Lys Val Val Ser Met Cys Cys 20 25 30 ggc ttt ggg gtt ttt ggc aac tgt tac ctt gtg cca aac cat atg ttt 144 Gly Phe Gly Val Phe Gly Asn Cys Tyr Leu Val Pro Asn His Met Phe 35 40 45 gag gag act ttt gac aca att ttg ttg ggg gag act ccc ctg aag aag 192 Glu Glu Thr Phe Asp Thr Ile Leu Leu Gly Glu Thr Pro Leu Lys Lys 50 55 60 aag gat tac gag gtt att aac ctc gaa aca ggc gat ggt gtt tct gat 240 Lys Asp Tyr Glu Val Ile Asn Leu Glu Thr Gly Asp Gly Val Ser Asp 65 70 75 80 gca gca ctg ttg cat gtt ttt aaa ggc ccc aga gtt aag gac atg aca 288 Ala Ala Leu Leu His Val Phe Lys Gly Pro Arg Val Lys Asp Met Thr 85 90 95 atg cac ttc aga gat gaa gta cgc att ccc aag ggg aca act gtt gca 336 Met His Phe Arg Asp Glu Val Arg Ile Pro Lys Gly Thr Thr Val Ala 100 105 110 ggc tgt gtg aac agt cac gag ttt ggg cgc ttg gtt ttt act ggt acc 384 Gly Cys Val Asn Ser His Glu Phe Gly Arg Leu Val Phe Thr Gly Thr 115 120 125 gcc ctt aca ttt aaa gat gtg att gtg tgt tca gac ggt gat gag ctt 432 Ala Leu Thr Phe Lys Asp Val Ile Val Cys Ser Asp Gly Asp Glu Leu 130 135 140 ccg aac gtg ttt gcc tac aaa gct gcc aca caa cgg ggc tac tgt ggc 480 Pro Asn Val Phe Ala Tyr Lys Ala Ala Thr Gln Arg Gly Tyr Cys Gly 145 150 155 160 agt ccc gtt tta gtg aag aac agt gcg cac aca gtg gtg gtt ggg att 528 Ser Pro Val Leu Val Lys Asn Ser Ala His Thr Val Val Val Gly Ile 165 170 175 cac tct gca ggt ggg aac gga aac ggg tac gcg tcg tgt gtc acc cga 576 His Ser Ala Gly Gly Asn Gly Asn Gly Tyr Ala Ser Cys Val Thr Arg 180 185 190 tct gtc ctg ttg aag gtt aag aga atg ctt gat cct gat gtc cat ctt 624 Ser Val Leu Leu Lys Val Lys Arg Met Leu Asp Pro Asp Val His Leu 195 200 205 gag 627 Glu <210> 412 <211> 209 <212> PRT <213> Bovine rhinitis <400> 412 Ser Gly Ala Pro Pro Thr Asp Met Gln Gln His Val Leu Arg Asn Val 1 5 10 15 Arg Pro Ile Ser Leu Val Pro Asp Gly Lys Val Val Ser Met Cys Cys 20 25 30 Gly Phe Gly Val Phe Gly Asn Cys Tyr Leu Val Pro Asn His Met Phe 35 40 45 Glu Glu Thr Phe Asp Thr Ile Leu Leu Gly Glu Thr Pro Leu Lys Lys 50 55 60 Lys Asp Tyr Glu Val Ile Asn Leu Glu Thr Gly Asp Gly Val Ser Asp 65 70 75 80 Ala Ala Leu Leu His Val Phe Lys Gly Pro Arg Val Lys Asp Met Thr 85 90 95 Met His Phe Arg Asp Glu Val Arg Ile Pro Lys Gly Thr Thr Val Ala 100 105 110 Gly Cys Val Asn Ser His Glu Phe Gly Arg Leu Val Phe Thr Gly Thr 115 120 125 Ala Leu Thr Phe Lys Asp Val Ile Val Cys Ser Asp Gly Asp Glu Leu 130 135 140 Pro Asn Val Phe Ala Tyr Lys Ala Ala Thr Gln Arg Gly Tyr Cys Gly 145 150 155 160 Ser Pro Val Leu Val Lys Asn Ser Ala His Thr Val Val Val Gly Ile 165 170 175 His Ser Ala Gly Gly Asn Gly Asn Gly Tyr Ala Ser Cys Val Thr Arg 180 185 190 Ser Val Leu Leu Lys Val Lys Arg Met Leu Asp Pro Asp Val His Leu 195 200 205 Glu <210> 413 <211> 627 <212> DNA <213> Bovine rhinitis <220> <221> CDS <222> (1)..(627) <220> <221> misc_feature <222> (1)..(627) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(627) <223> Bovine_Rhinitis_A_Sd-1_KP236128_3C(F120P) <400> 413 agt ggt gct cca cca aca gac atg cag caa cac gtt ctc agg aac gtg 48 Ser Gly Ala Pro Pro Thr Asp Met Gln Gln His Val Leu Arg Asn Val 1 5 10 15 cgc cca ata tca ctt gtt tgt gat gga aag gtt gtg tct atg tgc tgt 96 Arg Pro Ile Ser Leu Val Cys Asp Gly Lys Val Val Ser Met Cys Cys 20 25 30 ggc ttt ggg gtt ttt ggc aac tgt tac ctt gtg cca aac cat atg ttt 144 Gly Phe Gly Val Phe Gly Asn Cys Tyr Leu Val Pro Asn His Met Phe 35 40 45 gag gag act ttt gac aca att ttg ttg ggg gag act ccc ctg aag aag 192 Glu Glu Thr Phe Asp Thr Ile Leu Leu Gly Glu Thr Pro Leu Lys Lys 50 55 60 aag gat tac gag gtt att aac ctc gaa aca ggc gat ggt gtt tct gat 240 Lys Asp Tyr Glu Val Ile Asn Leu Glu Thr Gly Asp Gly Val Ser Asp 65 70 75 80 gca gca ctg ttg cat gtt ttt aaa ggc ccc aga gtt aag gac atg aca 288 Ala Ala Leu Leu His Val Phe Lys Gly Pro Arg Val Lys Asp Met Thr 85 90 95 atg cac ttc aga gat gaa gta cgc att ccc aag ggg aca act gtt gca 336 Met His Phe Arg Asp Glu Val Arg Ile Pro Lys Gly Thr Thr Val Ala 100 105 110 ggc tgt gtg aac agt cac gag ccn ggg cgc ttg gtt ttt act ggt acc 384 Gly Cys Val Asn Ser His Glu Pro Gly Arg Leu Val Phe Thr Gly Thr 115 120 125 gcc ctt aca ttt aaa gat gtg att gtg tgt tca gac ggt gat gag ctt 432 Ala Leu Thr Phe Lys Asp Val Ile Val Cys Ser Asp Gly Asp Glu Leu 130 135 140 ccg aac gtg ttt gcc tac aaa gct gcc aca caa cgg ggc tac tgt ggc 480 Pro Asn Val Phe Ala Tyr Lys Ala Ala Thr Gln Arg Gly Tyr Cys Gly 145 150 155 160 agt ccc gtt tta gtg aag aac agt gcg cac aca gtg gtg gtt ggg att 528 Ser Pro Val Leu Val Lys Asn Ser Ala His Thr Val Val Val Gly Ile 165 170 175 cac tct gca ggt ggg aac gga aac ggg tac gcg tcg tgt gtc acc cga 576 His Ser Ala Gly Gly Asn Gly Asn Gly Tyr Ala Ser Cys Val Thr Arg 180 185 190 tct gtc ctg ttg aag gtt aag aga atg ctt gat cct gat gtc cat ctt 624 Ser Val Leu Leu Lys Val Lys Arg Met Leu Asp Pro Asp Val His Leu 195 200 205 gag 627 Glu <210> 414 <211> 209 <212> PRT <213> Bovine rhinitis <400> 414 Ser Gly Ala Pro Pro Thr Asp Met Gln Gln His Val Leu Arg Asn Val 1 5 10 15 Arg Pro Ile Ser Leu Val Cys Asp Gly Lys Val Val Ser Met Cys Cys 20 25 30 Gly Phe Gly Val Phe Gly Asn Cys Tyr Leu Val Pro Asn His Met Phe 35 40 45 Glu Glu Thr Phe Asp Thr Ile Leu Leu Gly Glu Thr Pro Leu Lys Lys 50 55 60 Lys Asp Tyr Glu Val Ile Asn Leu Glu Thr Gly Asp Gly Val Ser Asp 65 70 75 80 Ala Ala Leu Leu His Val Phe Lys Gly Pro Arg Val Lys Asp Met Thr 85 90 95 Met His Phe Arg Asp Glu Val Arg Ile Pro Lys Gly Thr Thr Val Ala 100 105 110 Gly Cys Val Asn Ser His Glu Pro Gly Arg Leu Val Phe Thr Gly Thr 115 120 125 Ala Leu Thr Phe Lys Asp Val Ile Val Cys Ser Asp Gly Asp Glu Leu 130 135 140 Pro Asn Val Phe Ala Tyr Lys Ala Ala Thr Gln Arg Gly Tyr Cys Gly 145 150 155 160 Ser Pro Val Leu Val Lys Asn Ser Ala His Thr Val Val Val Gly Ile 165 170 175 His Ser Ala Gly Gly Asn Gly Asn Gly Tyr Ala Ser Cys Val Thr Arg 180 185 190 Ser Val Leu Leu Lys Val Lys Arg Met Leu Asp Pro Asp Val His Leu 195 200 205 Glu <210> 415 <211> 627 <212> DNA <213> Bovine rhinitis <220> <221> CDS <222> (1)..(627) <220> <221> misc_feature <222> (1)..(627) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(627) <223> Bovine_Rhinitis_A_Sd-1_KP236128_3C(L123P) <400> 415 agt ggt gct cca cca aca gac atg cag caa cac gtt ctc agg aac gtg 48 Ser Gly Ala Pro Pro Thr Asp Met Gln Gln His Val Leu Arg Asn Val 1 5 10 15 cgc cca ata tca ctt gtt tgt gat gga aag gtt gtg tct atg tgc tgt 96 Arg Pro Ile Ser Leu Val Cys Asp Gly Lys Val Val Ser Met Cys Cys 20 25 30 ggc ttt ggg gtt ttt ggc aac tgt tac ctt gtg cca aac cat atg ttt 144 Gly Phe Gly Val Phe Gly Asn Cys Tyr Leu Val Pro Asn His Met Phe 35 40 45 gag gag act ttt gac aca att ttg ttg ggg gag act ccc ctg aag aag 192 Glu Glu Thr Phe Asp Thr Ile Leu Leu Gly Glu Thr Pro Leu Lys Lys 50 55 60 aag gat tac gag gtt att aac ctc gaa aca ggc gat ggt gtt tct gat 240 Lys Asp Tyr Glu Val Ile Asn Leu Glu Thr Gly Asp Gly Val Ser Asp 65 70 75 80 gca gca ctg ttg cat gtt ttt aaa ggc ccc aga gtt aag gac atg aca 288 Ala Ala Leu Leu His Val Phe Lys Gly Pro Arg Val Lys Asp Met Thr 85 90 95 atg cac ttc aga gat gaa gta cgc att ccc aag ggg aca act gtt gca 336 Met His Phe Arg Asp Glu Val Arg Ile Pro Lys Gly Thr Thr Val Ala 100 105 110 ggc tgt gtg aac agt cac gag ttt ggg cgc ccn gtt ttt act ggt acc 384 Gly Cys Val Asn Ser His Glu Phe Gly Arg Pro Val Phe Thr Gly Thr 115 120 125 gcc ctt aca ttt aaa gat gtg att gtg tgt tca gac ggt gat gag ctt 432 Ala Leu Thr Phe Lys Asp Val Ile Val Cys Ser Asp Gly Asp Glu Leu 130 135 140 ccg aac gtg ttt gcc tac aaa gct gcc aca caa cgg ggc tac tgt ggc 480 Pro Asn Val Phe Ala Tyr Lys Ala Ala Thr Gln Arg Gly Tyr Cys Gly 145 150 155 160 agt ccc gtt tta gtg aag aac agt gcg cac aca gtg gtg gtt ggg att 528 Ser Pro Val Leu Val Lys Asn Ser Ala His Thr Val Val Val Gly Ile 165 170 175 cac tct gca ggt ggg aac gga aac ggg tac gcg tcg tgt gtc acc cga 576 His Ser Ala Gly Gly Asn Gly Asn Gly Tyr Ala Ser Cys Val Thr Arg 180 185 190 tct gtc ctg ttg aag gtt aag aga atg ctt gat cct gat gtc cat ctt 624 Ser Val Leu Leu Lys Val Lys Arg Met Leu Asp Pro Asp Val His Leu 195 200 205 gag 627 Glu <210> 416 <211> 209 <212> PRT <213> Bovine rhinitis <400> 416 Ser Gly Ala Pro Pro Thr Asp Met Gln Gln His Val Leu Arg Asn Val 1 5 10 15 Arg Pro Ile Ser Leu Val Cys Asp Gly Lys Val Val Ser Met Cys Cys 20 25 30 Gly Phe Gly Val Phe Gly Asn Cys Tyr Leu Val Pro Asn His Met Phe 35 40 45 Glu Glu Thr Phe Asp Thr Ile Leu Leu Gly Glu Thr Pro Leu Lys Lys 50 55 60 Lys Asp Tyr Glu Val Ile Asn Leu Glu Thr Gly Asp Gly Val Ser Asp 65 70 75 80 Ala Ala Leu Leu His Val Phe Lys Gly Pro Arg Val Lys Asp Met Thr 85 90 95 Met His Phe Arg Asp Glu Val Arg Ile Pro Lys Gly Thr Thr Val Ala 100 105 110 Gly Cys Val Asn Ser His Glu Phe Gly Arg Pro Val Phe Thr Gly Thr 115 120 125 Ala Leu Thr Phe Lys Asp Val Ile Val Cys Ser Asp Gly Asp Glu Leu 130 135 140 Pro Asn Val Phe Ala Tyr Lys Ala Ala Thr Gln Arg Gly Tyr Cys Gly 145 150 155 160 Ser Pro Val Leu Val Lys Asn Ser Ala His Thr Val Val Val Gly Ile 165 170 175 His Ser Ala Gly Gly Asn Gly Asn Gly Tyr Ala Ser Cys Val Thr Arg 180 185 190 Ser Val Leu Leu Lys Val Lys Arg Met Leu Asp Pro Asp Val His Leu 195 200 205 Glu <210> 417 <211> 627 <212> DNA <213> Bovine rhinitis <220> <221> CDS <222> (1)..(627) <220> <221> misc_feature <222> (1)..(627) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(627) <223> Bovine_Rhinitis_A_Sd-1_KP236128_3C(T96P) <400> 417 agt ggt gct cca cca aca gac atg cag caa cac gtt ctc agg aac gtg 48 Ser Gly Ala Pro Pro Thr Asp Met Gln Gln His Val Leu Arg Asn Val 1 5 10 15 cgc cca ata tca ctt gtt tgt gat gga aag gtt gtg tct atg tgc tgt 96 Arg Pro Ile Ser Leu Val Cys Asp Gly Lys Val Val Ser Met Cys Cys 20 25 30 ggc ttt ggg gtt ttt ggc aac tgt tac ctt gtg cca aac cat atg ttt 144 Gly Phe Gly Val Phe Gly Asn Cys Tyr Leu Val Pro Asn His Met Phe 35 40 45 gag gag act ttt gac aca att ttg ttg ggg gag act ccc ctg aag aag 192 Glu Glu Thr Phe Asp Thr Ile Leu Leu Gly Glu Thr Pro Leu Lys Lys 50 55 60 aag gat tac gag gtt att aac ctc gaa aca ggc gat ggt gtt tct gat 240 Lys Asp Tyr Glu Val Ile Asn Leu Glu Thr Gly Asp Gly Val Ser Asp 65 70 75 80 gca gca ctg ttg cat gtt ttt aaa ggc ccc aga gtt aag gac atg ccn 288 Ala Ala Leu Leu His Val Phe Lys Gly Pro Arg Val Lys Asp Met Pro 85 90 95 atg cac ttc aga gat gaa gta cgc att ccc aag ggg aca act gtt gca 336 Met His Phe Arg Asp Glu Val Arg Ile Pro Lys Gly Thr Thr Val Ala 100 105 110 ggc tgt gtg aac agt cac gag ttt ggg cgc ttg gtt ttt act ggt acc 384 Gly Cys Val Asn Ser His Glu Phe Gly Arg Leu Val Phe Thr Gly Thr 115 120 125 gcc ctt aca ttt aaa gat gtg att gtg tgt tca gac ggt gat gag ctt 432 Ala Leu Thr Phe Lys Asp Val Ile Val Cys Ser Asp Gly Asp Glu Leu 130 135 140 ccg aac gtg ttt gcc tac aaa gct gcc aca caa cgg ggc tac tgt ggc 480 Pro Asn Val Phe Ala Tyr Lys Ala Ala Thr Gln Arg Gly Tyr Cys Gly 145 150 155 160 agt ccc gtt tta gtg aag aac agt gcg cac aca gtg gtg gtt ggg att 528 Ser Pro Val Leu Val Lys Asn Ser Ala His Thr Val Val Val Gly Ile 165 170 175 cac tct gca ggt ggg aac gga aac ggg tac gcg tcg tgt gtc acc cga 576 His Ser Ala Gly Gly Asn Gly Asn Gly Tyr Ala Ser Cys Val Thr Arg 180 185 190 tct gtc ctg ttg aag gtt aag aga atg ctt gat cct gat gtc cat ctt 624 Ser Val Leu Leu Lys Val Lys Arg Met Leu Asp Pro Asp Val His Leu 195 200 205 gag 627 Glu <210> 418 <211> 209 <212> PRT <213> Bovine rhinitis <400> 418 Ser Gly Ala Pro Pro Thr Asp Met Gln Gln His Val Leu Arg Asn Val 1 5 10 15 Arg Pro Ile Ser Leu Val Cys Asp Gly Lys Val Val Ser Met Cys Cys 20 25 30 Gly Phe Gly Val Phe Gly Asn Cys Tyr Leu Val Pro Asn His Met Phe 35 40 45 Glu Glu Thr Phe Asp Thr Ile Leu Leu Gly Glu Thr Pro Leu Lys Lys 50 55 60 Lys Asp Tyr Glu Val Ile Asn Leu Glu Thr Gly Asp Gly Val Ser Asp 65 70 75 80 Ala Ala Leu Leu His Val Phe Lys Gly Pro Arg Val Lys Asp Met Pro 85 90 95 Met His Phe Arg Asp Glu Val Arg Ile Pro Lys Gly Thr Thr Val Ala 100 105 110 Gly Cys Val Asn Ser His Glu Phe Gly Arg Leu Val Phe Thr Gly Thr 115 120 125 Ala Leu Thr Phe Lys Asp Val Ile Val Cys Ser Asp Gly Asp Glu Leu 130 135 140 Pro Asn Val Phe Ala Tyr Lys Ala Ala Thr Gln Arg Gly Tyr Cys Gly 145 150 155 160 Ser Pro Val Leu Val Lys Asn Ser Ala His Thr Val Val Val Gly Ile 165 170 175 His Ser Ala Gly Gly Asn Gly Asn Gly Tyr Ala Ser Cys Val Thr Arg 180 185 190 Ser Val Leu Leu Lys Val Lys Arg Met Leu Asp Pro Asp Val His Leu 195 200 205 Glu <210> 419 <211> 627 <212> DNA <213> Bovine rhinitis <220> <221> CDS <222> (1)..(627) <220> <221> misc_feature <222> (1)..(627) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(627) <223> Bovine_Rhinitis_A_Sd-1_KP236128_3C(V22P) <400> 419 agt ggt gct cca cca aca gac atg cag caa cac gtt ctc agg aac gtg 48 Ser Gly Ala Pro Pro Thr Asp Met Gln Gln His Val Leu Arg Asn Val 1 5 10 15 cgc cca ata tca ctt ccn tgt gat gga aag gtt gtg tct atg tgc tgt 96 Arg Pro Ile Ser Leu Pro Cys Asp Gly Lys Val Val Ser Met Cys Cys 20 25 30 ggc ttt ggg gtt ttt ggc aac tgt tac ctt gtg cca aac cat atg ttt 144 Gly Phe Gly Val Phe Gly Asn Cys Tyr Leu Val Pro Asn His Met Phe 35 40 45 gag gag act ttt gac aca att ttg ttg ggg gag act ccc ctg aag aag 192 Glu Glu Thr Phe Asp Thr Ile Leu Leu Gly Glu Thr Pro Leu Lys Lys 50 55 60 aag gat tac gag gtt att aac ctc gaa aca ggc gat ggt gtt tct gat 240 Lys Asp Tyr Glu Val Ile Asn Leu Glu Thr Gly Asp Gly Val Ser Asp 65 70 75 80 gca gca ctg ttg cat gtt ttt aaa ggc ccc aga gtt aag gac atg aca 288 Ala Ala Leu Leu His Val Phe Lys Gly Pro Arg Val Lys Asp Met Thr 85 90 95 atg cac ttc aga gat gaa gta cgc att ccc aag ggg aca act gtt gca 336 Met His Phe Arg Asp Glu Val Arg Ile Pro Lys Gly Thr Thr Val Ala 100 105 110 ggc tgt gtg aac agt cac gag ttt ggg cgc ttg gtt ttt act ggt acc 384 Gly Cys Val Asn Ser His Glu Phe Gly Arg Leu Val Phe Thr Gly Thr 115 120 125 gcc ctt aca ttt aaa gat gtg att gtg tgt tca gac ggt gat gag ctt 432 Ala Leu Thr Phe Lys Asp Val Ile Val Cys Ser Asp Gly Asp Glu Leu 130 135 140 ccg aac gtg ttt gcc tac aaa gct gcc aca caa cgg ggc tac tgt ggc 480 Pro Asn Val Phe Ala Tyr Lys Ala Ala Thr Gln Arg Gly Tyr Cys Gly 145 150 155 160 agt ccc gtt tta gtg aag aac agt gcg cac aca gtg gtg gtt ggg att 528 Ser Pro Val Leu Val Lys Asn Ser Ala His Thr Val Val Val Gly Ile 165 170 175 cac tct gca ggt ggg aac gga aac ggg tac gcg tcg tgt gtc acc cga 576 His Ser Ala Gly Gly Asn Gly Asn Gly Tyr Ala Ser Cys Val Thr Arg 180 185 190 tct gtc ctg ttg aag gtt aag aga atg ctt gat cct gat gtc cat ctt 624 Ser Val Leu Leu Lys Val Lys Arg Met Leu Asp Pro Asp Val His Leu 195 200 205 gag 627 Glu <210> 420 <211> 209 <212> PRT <213> Bovine rhinitis <400> 420 Ser Gly Ala Pro Pro Thr Asp Met Gln Gln His Val Leu Arg Asn Val 1 5 10 15 Arg Pro Ile Ser Leu Pro Cys Asp Gly Lys Val Val Ser Met Cys Cys 20 25 30 Gly Phe Gly Val Phe Gly Asn Cys Tyr Leu Val Pro Asn His Met Phe 35 40 45 Glu Glu Thr Phe Asp Thr Ile Leu Leu Gly Glu Thr Pro Leu Lys Lys 50 55 60 Lys Asp Tyr Glu Val Ile Asn Leu Glu Thr Gly Asp Gly Val Ser Asp 65 70 75 80 Ala Ala Leu Leu His Val Phe Lys Gly Pro Arg Val Lys Asp Met Thr 85 90 95 Met His Phe Arg Asp Glu Val Arg Ile Pro Lys Gly Thr Thr Val Ala 100 105 110 Gly Cys Val Asn Ser His Glu Phe Gly Arg Leu Val Phe Thr Gly Thr 115 120 125 Ala Leu Thr Phe Lys Asp Val Ile Val Cys Ser Asp Gly Asp Glu Leu 130 135 140 Pro Asn Val Phe Ala Tyr Lys Ala Ala Thr Gln Arg Gly Tyr Cys Gly 145 150 155 160 Ser Pro Val Leu Val Lys Asn Ser Ala His Thr Val Val Val Gly Ile 165 170 175 His Ser Ala Gly Gly Asn Gly Asn Gly Tyr Ala Ser Cys Val Thr Arg 180 185 190 Ser Val Leu Leu Lys Val Lys Arg Met Leu Asp Pro Asp Val His Leu 195 200 205 Glu <210> 421 <211> 627 <212> DNA <213> Bovine rhinitis <220> <221> CDS <222> (1)..(627) <220> <221> misc_feature <222> (1)..(627) <223> Bovine_Rhinitis_A_Sd-1_KP236128_3C(wt) <400> 421 agt ggt gct cca cca aca gac atg cag caa cac gtt ctc agg aac gtg 48 Ser Gly Ala Pro Pro Thr Asp Met Gln Gln His Val Leu Arg Asn Val 1 5 10 15 cgc cca ata tca ctt gtt tgt gat gga aag gtt gtg tct atg tgc tgt 96 Arg Pro Ile Ser Leu Val Cys Asp Gly Lys Val Val Ser Met Cys Cys 20 25 30 ggc ttt ggg gtt ttt ggc aac tgt tac ctt gtg cca aac cat atg ttt 144 Gly Phe Gly Val Phe Gly Asn Cys Tyr Leu Val Pro Asn His Met Phe 35 40 45 gag gag act ttt gac aca att ttg ttg ggg gag act ccc ctg aag aag 192 Glu Glu Thr Phe Asp Thr Ile Leu Leu Gly Glu Thr Pro Leu Lys Lys 50 55 60 aag gat tac gag gtt att aac ctc gaa aca ggc gat ggt gtt tct gat 240 Lys Asp Tyr Glu Val Ile Asn Leu Glu Thr Gly Asp Gly Val Ser Asp 65 70 75 80 gca gca ctg ttg cat gtt ttt aaa ggc ccc aga gtt aag gac atg aca 288 Ala Ala Leu Leu His Val Phe Lys Gly Pro Arg Val Lys Asp Met Thr 85 90 95 atg cac ttc aga gat gaa gta cgc att ccc aag ggg aca act gtt gca 336 Met His Phe Arg Asp Glu Val Arg Ile Pro Lys Gly Thr Thr Val Ala 100 105 110 ggc tgt gtg aac agt cac gag ttt ggg cgc ttg gtt ttt act ggt acc 384 Gly Cys Val Asn Ser His Glu Phe Gly Arg Leu Val Phe Thr Gly Thr 115 120 125 gcc ctt aca ttt aaa gat gtg att gtg tgt tca gac ggt gat gag ctt 432 Ala Leu Thr Phe Lys Asp Val Ile Val Cys Ser Asp Gly Asp Glu Leu 130 135 140 ccg aac gtg ttt gcc tac aaa gct gcc aca caa cgg ggc tac tgt ggc 480 Pro Asn Val Phe Ala Tyr Lys Ala Ala Thr Gln Arg Gly Tyr Cys Gly 145 150 155 160 agt ccc gtt tta gtg aag aac agt gcg cac aca gtg gtg gtt ggg att 528 Ser Pro Val Leu Val Lys Asn Ser Ala His Thr Val Val Val Gly Ile 165 170 175 cac tct gca ggt ggg aac gga aac ggg tac gcg tcg tgt gtc acc cga 576 His Ser Ala Gly Gly Asn Gly Asn Gly Tyr Ala Ser Cys Val Thr Arg 180 185 190 tct gtc ctg ttg aag gtt aag aga atg ctt gat cct gat gtc cat ctt 624 Ser Val Leu Leu Lys Val Lys Arg Met Leu Asp Pro Asp Val His Leu 195 200 205 gag 627 Glu <210> 422 <211> 209 <212> PRT <213> Bovine rhinitis <400> 422 Ser Gly Ala Pro Pro Thr Asp Met Gln Gln His Val Leu Arg Asn Val 1 5 10 15 Arg Pro Ile Ser Leu Val Cys Asp Gly Lys Val Val Ser Met Cys Cys 20 25 30 Gly Phe Gly Val Phe Gly Asn Cys Tyr Leu Val Pro Asn His Met Phe 35 40 45 Glu Glu Thr Phe Asp Thr Ile Leu Leu Gly Glu Thr Pro Leu Lys Lys 50 55 60 Lys Asp Tyr Glu Val Ile Asn Leu Glu Thr Gly Asp Gly Val Ser Asp 65 70 75 80 Ala Ala Leu Leu His Val Phe Lys Gly Pro Arg Val Lys Asp Met Thr 85 90 95 Met His Phe Arg Asp Glu Val Arg Ile Pro Lys Gly Thr Thr Val Ala 100 105 110 Gly Cys Val Asn Ser His Glu Phe Gly Arg Leu Val Phe Thr Gly Thr 115 120 125 Ala Leu Thr Phe Lys Asp Val Ile Val Cys Ser Asp Gly Asp Glu Leu 130 135 140 Pro Asn Val Phe Ala Tyr Lys Ala Ala Thr Gln Arg Gly Tyr Cys Gly 145 150 155 160 Ser Pro Val Leu Val Lys Asn Ser Ala His Thr Val Val Val Gly Ile 165 170 175 His Ser Ala Gly Gly Asn Gly Asn Gly Tyr Ala Ser Cys Val Thr Arg 180 185 190 Ser Val Leu Leu Lys Val Lys Arg Met Leu Asp Pro Asp Val His Leu 195 200 205 Glu <210> 423 <211> 624 <212> DNA <213> Bovine rhinitis <220> <221> CDS <222> (1)..(624) <220> <221> misc_feature <222> (1)..(624) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(624) <223> Bovine_Rhinitus_B_3C(L124P) <400> 423 agt ggg aac cct cca act gac atg cag ttg gtg gta ctc aag aac aca 48 Ser Gly Asn Pro Pro Thr Asp Met Gln Leu Val Val Leu Lys Asn Thr 1 5 10 15 caa cca att tca cta gtt aga gat gga caa att gtc gcc act tgt tgt 96 Gln Pro Ile Ser Leu Val Arg Asp Gly Gln Ile Val Ala Thr Cys Cys 20 25 30 gct ctt ggc gtt ttt gga acc acc tat tta gtt ccc tac cac ctc ttt 144 Ala Leu Gly Val Phe Gly Thr Thr Tyr Leu Val Pro Tyr His Leu Phe 35 40 45 gaa gaa agt ttt gac acc ctc att ata gga gac aga cat ctg aaa gaa 192 Glu Glu Ser Phe Asp Thr Leu Ile Ile Gly Asp Arg His Leu Lys Glu 50 55 60 caa gac tac aaa ctt gac act ttt gaa ctc aga gac ggg aaa atc agc 240 Gln Asp Tyr Lys Leu Asp Thr Phe Glu Leu Arg Asp Gly Lys Ile Ser 65 70 75 80 gat gtt gct gcc ctc acc tta aac aaa gga gcc cgt gtg cgt gac att 288 Asp Val Ala Ala Leu Thr Leu Asn Lys Gly Ala Arg Val Arg Asp Ile 85 90 95 acc tca cac ttt cga gac gag gtc aag gtc act aag aat tca cca gtt 336 Thr Ser His Phe Arg Asp Glu Val Lys Val Thr Lys Asn Ser Pro Val 100 105 110 gtc ggt tgt gtc aag aac aca acg gtc ggt caa ccn gta ttt aac ggg 384 Val Gly Cys Val Lys Asn Thr Thr Val Gly Gln Pro Val Phe Asn Gly 115 120 125 aca gct gct ggt ttt aaa gac cat atc atc tgc tct gat ggt gac act 432 Thr Ala Ala Gly Phe Lys Asp His Ile Ile Cys Ser Asp Gly Asp Thr 130 135 140 ctg cct aat atg ttc gtt tac act gcc aac aca cag tac ggg tat tgc 480 Leu Pro Asn Met Phe Val Tyr Thr Ala Asn Thr Gln Tyr Gly Tyr Cys 145 150 155 160 gga agc ggc ata ctc gtc aaa gat ggt tct cac aca gtc atc ata ggc 528 Gly Ser Gly Ile Leu Val Lys Asp Gly Ser His Thr Val Ile Ile Gly 165 170 175 atc cat agt gca gga ggt aac gga aaa ggt tac gct agt tgt gtc act 576 Ile His Ser Ala Gly Gly Asn Gly Lys Gly Tyr Ala Ser Cys Val Thr 180 185 190 cgt tcc gca ctc ctc gca ctg agg ggc aga gaa agg ccc gag ctc gaa 624 Arg Ser Ala Leu Leu Ala Leu Arg Gly Arg Glu Arg Pro Glu Leu Glu 195 200 205 <210> 424 <211> 208 <212> PRT <213> Bovine rhinitis <400> 424 Ser Gly Asn Pro Pro Thr Asp Met Gln Leu Val Val Leu Lys Asn Thr 1 5 10 15 Gln Pro Ile Ser Leu Val Arg Asp Gly Gln Ile Val Ala Thr Cys Cys 20 25 30 Ala Leu Gly Val Phe Gly Thr Thr Tyr Leu Val Pro Tyr His Leu Phe 35 40 45 Glu Glu Ser Phe Asp Thr Leu Ile Ile Gly Asp Arg His Leu Lys Glu 50 55 60 Gln Asp Tyr Lys Leu Asp Thr Phe Glu Leu Arg Asp Gly Lys Ile Ser 65 70 75 80 Asp Val Ala Ala Leu Thr Leu Asn Lys Gly Ala Arg Val Arg Asp Ile 85 90 95 Thr Ser His Phe Arg Asp Glu Val Lys Val Thr Lys Asn Ser Pro Val 100 105 110 Val Gly Cys Val Lys Asn Thr Thr Val Gly Gln Pro Val Phe Asn Gly 115 120 125 Thr Ala Ala Gly Phe Lys Asp His Ile Ile Cys Ser Asp Gly Asp Thr 130 135 140 Leu Pro Asn Met Phe Val Tyr Thr Ala Asn Thr Gln Tyr Gly Tyr Cys 145 150 155 160 Gly Ser Gly Ile Leu Val Lys Asp Gly Ser His Thr Val Ile Ile Gly 165 170 175 Ile His Ser Ala Gly Gly Asn Gly Lys Gly Tyr Ala Ser Cys Val Thr 180 185 190 Arg Ser Ala Leu Leu Ala Leu Arg Gly Arg Glu Arg Pro Glu Leu Glu 195 200 205 <210> 425 <211> 624 <212> DNA <213> Bovine rhinitis <220> <221> CDS <222> (1)..(624) <220> <221> misc_feature <222> (1)..(624) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(624) <223> Bovine_Rhinitus_B_3C(R23P) <400> 425 agt ggg aac cct cca act gac atg cag ttg gtg gta ctc aag aac aca 48 Ser Gly Asn Pro Pro Thr Asp Met Gln Leu Val Val Leu Lys Asn Thr 1 5 10 15 caa cca att tca cta gtt ccn gat gga caa att gtc gcc act tgt tgt 96 Gln Pro Ile Ser Leu Val Pro Asp Gly Gln Ile Val Ala Thr Cys Cys 20 25 30 gct ctt ggc gtt ttt gga acc acc tat tta gtt ccc tac cac ctc ttt 144 Ala Leu Gly Val Phe Gly Thr Thr Tyr Leu Val Pro Tyr His Leu Phe 35 40 45 gaa gaa agt ttt gac acc ctc att ata gga gac aga cat ctg aaa gaa 192 Glu Glu Ser Phe Asp Thr Leu Ile Ile Gly Asp Arg His Leu Lys Glu 50 55 60 caa gac tac aaa ctt gac act ttt gaa ctc aga gac ggg aaa atc agc 240 Gln Asp Tyr Lys Leu Asp Thr Phe Glu Leu Arg Asp Gly Lys Ile Ser 65 70 75 80 gat gtt gct gcc ctc acc tta aac aaa gga gcc cgt gtg cgt gac att 288 Asp Val Ala Ala Leu Thr Leu Asn Lys Gly Ala Arg Val Arg Asp Ile 85 90 95 acc tca cac ttt cga gac gag gtc aag gtc act aag aat tca cca gtt 336 Thr Ser His Phe Arg Asp Glu Val Lys Val Thr Lys Asn Ser Pro Val 100 105 110 gtc ggt tgt gtc aag aac aca acg gtc ggt caa ctg gta ttt aac ggg 384 Val Gly Cys Val Lys Asn Thr Thr Val Gly Gln Leu Val Phe Asn Gly 115 120 125 aca gct gct ggt ttt aaa gac cat atc atc tgc tct gat ggt gac act 432 Thr Ala Ala Gly Phe Lys Asp His Ile Ile Cys Ser Asp Gly Asp Thr 130 135 140 ctg cct aat atg ttc gtt tac act gcc aac aca cag tac ggg tat tgc 480 Leu Pro Asn Met Phe Val Tyr Thr Ala Asn Thr Gln Tyr Gly Tyr Cys 145 150 155 160 gga agc ggc ata ctc gtc aaa gat ggt tct cac aca gtc atc ata ggc 528 Gly Ser Gly Ile Leu Val Lys Asp Gly Ser His Thr Val Ile Ile Gly 165 170 175 atc cat agt gca gga ggt aac gga aaa ggt tac gct agt tgt gtc act 576 Ile His Ser Ala Gly Gly Asn Gly Lys Gly Tyr Ala Ser Cys Val Thr 180 185 190 cgt tcc gca ctc ctc gca ctg agg ggc aga gaa agg ccc gag ctc gaa 624 Arg Ser Ala Leu Leu Ala Leu Arg Gly Arg Glu Arg Pro Glu Leu Glu 195 200 205 <210> 426 <211> 208 <212> PRT <213> Bovine rhinitis <400> 426 Ser Gly Asn Pro Pro Thr Asp Met Gln Leu Val Val Leu Lys Asn Thr 1 5 10 15 Gln Pro Ile Ser Leu Val Pro Asp Gly Gln Ile Val Ala Thr Cys Cys 20 25 30 Ala Leu Gly Val Phe Gly Thr Thr Tyr Leu Val Pro Tyr His Leu Phe 35 40 45 Glu Glu Ser Phe Asp Thr Leu Ile Ile Gly Asp Arg His Leu Lys Glu 50 55 60 Gln Asp Tyr Lys Leu Asp Thr Phe Glu Leu Arg Asp Gly Lys Ile Ser 65 70 75 80 Asp Val Ala Ala Leu Thr Leu Asn Lys Gly Ala Arg Val Arg Asp Ile 85 90 95 Thr Ser His Phe Arg Asp Glu Val Lys Val Thr Lys Asn Ser Pro Val 100 105 110 Val Gly Cys Val Lys Asn Thr Thr Val Gly Gln Leu Val Phe Asn Gly 115 120 125 Thr Ala Ala Gly Phe Lys Asp His Ile Ile Cys Ser Asp Gly Asp Thr 130 135 140 Leu Pro Asn Met Phe Val Tyr Thr Ala Asn Thr Gln Tyr Gly Tyr Cys 145 150 155 160 Gly Ser Gly Ile Leu Val Lys Asp Gly Ser His Thr Val Ile Ile Gly 165 170 175 Ile His Ser Ala Gly Gly Asn Gly Lys Gly Tyr Ala Ser Cys Val Thr 180 185 190 Arg Ser Ala Leu Leu Ala Leu Arg Gly Arg Glu Arg Pro Glu Leu Glu 195 200 205 <210> 427 <211> 624 <212> DNA <213> Bovine rhinitis <220> <221> CDS <222> (1)..(624) <220> <221> misc_feature <222> (1)..(624) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(624) <223> Bovine_Rhinitus_B_3C(T97P) <400> 427 agt ggg aac cct cca act gac atg cag ttg gtg gta ctc aag aac aca 48 Ser Gly Asn Pro Pro Thr Asp Met Gln Leu Val Val Leu Lys Asn Thr 1 5 10 15 caa cca att tca cta gtt aga gat gga caa att gtc gcc act tgt tgt 96 Gln Pro Ile Ser Leu Val Arg Asp Gly Gln Ile Val Ala Thr Cys Cys 20 25 30 gct ctt ggc gtt ttt gga acc acc tat tta gtt ccc tac cac ctc ttt 144 Ala Leu Gly Val Phe Gly Thr Thr Tyr Leu Val Pro Tyr His Leu Phe 35 40 45 gaa gaa agt ttt gac acc ctc att ata gga gac aga cat ctg aaa gaa 192 Glu Glu Ser Phe Asp Thr Leu Ile Ile Gly Asp Arg His Leu Lys Glu 50 55 60 caa gac tac aaa ctt gac act ttt gaa ctc aga gac ggg aaa atc agc 240 Gln Asp Tyr Lys Leu Asp Thr Phe Glu Leu Arg Asp Gly Lys Ile Ser 65 70 75 80 gat gtt gct gcc ctc acc tta aac aaa gga gcc cgt gtg cgt gac att 288 Asp Val Ala Ala Leu Thr Leu Asn Lys Gly Ala Arg Val Arg Asp Ile 85 90 95 ccn tca cac ttt cga gac gag gtc aag gtc act aag aat tca cca gtt 336 Pro Ser His Phe Arg Asp Glu Val Lys Val Thr Lys Asn Ser Pro Val 100 105 110 gtc ggt tgt gtc aag aac aca acg gtc ggt caa ctg gta ttt aac ggg 384 Val Gly Cys Val Lys Asn Thr Thr Val Gly Gln Leu Val Phe Asn Gly 115 120 125 aca gct gct ggt ttt aaa gac cat atc atc tgc tct gat ggt gac act 432 Thr Ala Ala Gly Phe Lys Asp His Ile Ile Cys Ser Asp Gly Asp Thr 130 135 140 ctg cct aat atg ttc gtt tac act gcc aac aca cag tac ggg tat tgc 480 Leu Pro Asn Met Phe Val Tyr Thr Ala Asn Thr Gln Tyr Gly Tyr Cys 145 150 155 160 gga agc ggc ata ctc gtc aaa gat ggt tct cac aca gtc atc ata ggc 528 Gly Ser Gly Ile Leu Val Lys Asp Gly Ser His Thr Val Ile Ile Gly 165 170 175 atc cat agt gca gga ggt aac gga aaa ggt tac gct agt tgt gtc act 576 Ile His Ser Ala Gly Gly Asn Gly Lys Gly Tyr Ala Ser Cys Val Thr 180 185 190 cgt tcc gca ctc ctc gca ctg agg ggc aga gaa agg ccc gag ctc gaa 624 Arg Ser Ala Leu Leu Ala Leu Arg Gly Arg Glu Arg Pro Glu Leu Glu 195 200 205 <210> 428 <211> 208 <212> PRT <213> Bovine rhinitis <400> 428 Ser Gly Asn Pro Pro Thr Asp Met Gln Leu Val Val Leu Lys Asn Thr 1 5 10 15 Gln Pro Ile Ser Leu Val Arg Asp Gly Gln Ile Val Ala Thr Cys Cys 20 25 30 Ala Leu Gly Val Phe Gly Thr Thr Tyr Leu Val Pro Tyr His Leu Phe 35 40 45 Glu Glu Ser Phe Asp Thr Leu Ile Ile Gly Asp Arg His Leu Lys Glu 50 55 60 Gln Asp Tyr Lys Leu Asp Thr Phe Glu Leu Arg Asp Gly Lys Ile Ser 65 70 75 80 Asp Val Ala Ala Leu Thr Leu Asn Lys Gly Ala Arg Val Arg Asp Ile 85 90 95 Pro Ser His Phe Arg Asp Glu Val Lys Val Thr Lys Asn Ser Pro Val 100 105 110 Val Gly Cys Val Lys Asn Thr Thr Val Gly Gln Leu Val Phe Asn Gly 115 120 125 Thr Ala Ala Gly Phe Lys Asp His Ile Ile Cys Ser Asp Gly Asp Thr 130 135 140 Leu Pro Asn Met Phe Val Tyr Thr Ala Asn Thr Gln Tyr Gly Tyr Cys 145 150 155 160 Gly Ser Gly Ile Leu Val Lys Asp Gly Ser His Thr Val Ile Ile Gly 165 170 175 Ile His Ser Ala Gly Gly Asn Gly Lys Gly Tyr Ala Ser Cys Val Thr 180 185 190 Arg Ser Ala Leu Leu Ala Leu Arg Gly Arg Glu Arg Pro Glu Leu Glu 195 200 205 <210> 429 <211> 624 <212> DNA <213> Bovine rhinitis <220> <221> CDS <222> (1)..(624) <220> <221> misc_feature <222> (1)..(624) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(624) <223> Bovine_Rhinitus_B_3C(V121P) <400> 429 agt ggg aac cct cca act gac atg cag ttg gtg gta ctc aag aac aca 48 Ser Gly Asn Pro Pro Thr Asp Met Gln Leu Val Val Leu Lys Asn Thr 1 5 10 15 caa cca att tca cta gtt aga gat gga caa att gtc gcc act tgt tgt 96 Gln Pro Ile Ser Leu Val Arg Asp Gly Gln Ile Val Ala Thr Cys Cys 20 25 30 gct ctt ggc gtt ttt gga acc acc tat tta gtt ccc tac cac ctc ttt 144 Ala Leu Gly Val Phe Gly Thr Thr Tyr Leu Val Pro Tyr His Leu Phe 35 40 45 gaa gaa agt ttt gac acc ctc att ata gga gac aga cat ctg aaa gaa 192 Glu Glu Ser Phe Asp Thr Leu Ile Ile Gly Asp Arg His Leu Lys Glu 50 55 60 caa gac tac aaa ctt gac act ttt gaa ctc aga gac ggg aaa atc agc 240 Gln Asp Tyr Lys Leu Asp Thr Phe Glu Leu Arg Asp Gly Lys Ile Ser 65 70 75 80 gat gtt gct gcc ctc acc tta aac aaa gga gcc cgt gtg cgt gac att 288 Asp Val Ala Ala Leu Thr Leu Asn Lys Gly Ala Arg Val Arg Asp Ile 85 90 95 acc tca cac ttt cga gac gag gtc aag gtc act aag aat tca cca gtt 336 Thr Ser His Phe Arg Asp Glu Val Lys Val Thr Lys Asn Ser Pro Val 100 105 110 gtc ggt tgt gtc aag aac aca acg ccn ggt caa ctg gta ttt aac ggg 384 Val Gly Cys Val Lys Asn Thr Thr Pro Gly Gln Leu Val Phe Asn Gly 115 120 125 aca gct gct ggt ttt aaa gac cat atc atc tgc tct gat ggt gac act 432 Thr Ala Ala Gly Phe Lys Asp His Ile Ile Cys Ser Asp Gly Asp Thr 130 135 140 ctg cct aat atg ttc gtt tac act gcc aac aca cag tac ggg tat tgc 480 Leu Pro Asn Met Phe Val Tyr Thr Ala Asn Thr Gln Tyr Gly Tyr Cys 145 150 155 160 gga agc ggc ata ctc gtc aaa gat ggt tct cac aca gtc atc ata ggc 528 Gly Ser Gly Ile Leu Val Lys Asp Gly Ser His Thr Val Ile Ile Gly 165 170 175 atc cat agt gca gga ggt aac gga aaa ggt tac gct agt tgt gtc act 576 Ile His Ser Ala Gly Gly Asn Gly Lys Gly Tyr Ala Ser Cys Val Thr 180 185 190 cgt tcc gca ctc ctc gca ctg agg ggc aga gaa agg ccc gag ctc gaa 624 Arg Ser Ala Leu Leu Ala Leu Arg Gly Arg Glu Arg Pro Glu Leu Glu 195 200 205 <210> 430 <211> 208 <212> PRT <213> Bovine rhinitis <400> 430 Ser Gly Asn Pro Pro Thr Asp Met Gln Leu Val Val Leu Lys Asn Thr 1 5 10 15 Gln Pro Ile Ser Leu Val Arg Asp Gly Gln Ile Val Ala Thr Cys Cys 20 25 30 Ala Leu Gly Val Phe Gly Thr Thr Tyr Leu Val Pro Tyr His Leu Phe 35 40 45 Glu Glu Ser Phe Asp Thr Leu Ile Ile Gly Asp Arg His Leu Lys Glu 50 55 60 Gln Asp Tyr Lys Leu Asp Thr Phe Glu Leu Arg Asp Gly Lys Ile Ser 65 70 75 80 Asp Val Ala Ala Leu Thr Leu Asn Lys Gly Ala Arg Val Arg Asp Ile 85 90 95 Thr Ser His Phe Arg Asp Glu Val Lys Val Thr Lys Asn Ser Pro Val 100 105 110 Val Gly Cys Val Lys Asn Thr Thr Pro Gly Gln Leu Val Phe Asn Gly 115 120 125 Thr Ala Ala Gly Phe Lys Asp His Ile Ile Cys Ser Asp Gly Asp Thr 130 135 140 Leu Pro Asn Met Phe Val Tyr Thr Ala Asn Thr Gln Tyr Gly Tyr Cys 145 150 155 160 Gly Ser Gly Ile Leu Val Lys Asp Gly Ser His Thr Val Ile Ile Gly 165 170 175 Ile His Ser Ala Gly Gly Asn Gly Lys Gly Tyr Ala Ser Cys Val Thr 180 185 190 Arg Ser Ala Leu Leu Ala Leu Arg Gly Arg Glu Arg Pro Glu Leu Glu 195 200 205 <210> 431 <211> 624 <212> DNA <213> Bovine rhinitis <220> <221> CDS <222> (1)..(624) <220> <221> misc_feature <222> (1)..(624) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(624) <223> Bovine_Rhinitus_B_3C(V22P) <400> 431 agt ggg aac cct cca act gac atg cag ttg gtg gta ctc aag aac aca 48 Ser Gly Asn Pro Pro Thr Asp Met Gln Leu Val Val Leu Lys Asn Thr 1 5 10 15 caa cca att tca cta ccn aga gat gga caa att gtc gcc act tgt tgt 96 Gln Pro Ile Ser Leu Pro Arg Asp Gly Gln Ile Val Ala Thr Cys Cys 20 25 30 gct ctt ggc gtt ttt gga acc acc tat tta gtt ccc tac cac ctc ttt 144 Ala Leu Gly Val Phe Gly Thr Thr Tyr Leu Val Pro Tyr His Leu Phe 35 40 45 gaa gaa agt ttt gac acc ctc att ata gga gac aga cat ctg aaa gaa 192 Glu Glu Ser Phe Asp Thr Leu Ile Ile Gly Asp Arg His Leu Lys Glu 50 55 60 caa gac tac aaa ctt gac act ttt gaa ctc aga gac ggg aaa atc agc 240 Gln Asp Tyr Lys Leu Asp Thr Phe Glu Leu Arg Asp Gly Lys Ile Ser 65 70 75 80 gat gtt gct gcc ctc acc tta aac aaa gga gcc cgt gtg cgt gac att 288 Asp Val Ala Ala Leu Thr Leu Asn Lys Gly Ala Arg Val Arg Asp Ile 85 90 95 acc tca cac ttt cga gac gag gtc aag gtc act aag aat tca cca gtt 336 Thr Ser His Phe Arg Asp Glu Val Lys Val Thr Lys Asn Ser Pro Val 100 105 110 gtc ggt tgt gtc aag aac aca acg gtc ggt caa ctg gta ttt aac ggg 384 Val Gly Cys Val Lys Asn Thr Thr Val Gly Gln Leu Val Phe Asn Gly 115 120 125 aca gct gct ggt ttt aaa gac cat atc atc tgc tct gat ggt gac act 432 Thr Ala Ala Gly Phe Lys Asp His Ile Ile Cys Ser Asp Gly Asp Thr 130 135 140 ctg cct aat atg ttc gtt tac act gcc aac aca cag tac ggg tat tgc 480 Leu Pro Asn Met Phe Val Tyr Thr Ala Asn Thr Gln Tyr Gly Tyr Cys 145 150 155 160 gga agc ggc ata ctc gtc aaa gat ggt tct cac aca gtc atc ata ggc 528 Gly Ser Gly Ile Leu Val Lys Asp Gly Ser His Thr Val Ile Ile Gly 165 170 175 atc cat agt gca gga ggt aac gga aaa ggt tac gct agt tgt gtc act 576 Ile His Ser Ala Gly Gly Asn Gly Lys Gly Tyr Ala Ser Cys Val Thr 180 185 190 cgt tcc gca ctc ctc gca ctg agg ggc aga gaa agg ccc gag ctc gaa 624 Arg Ser Ala Leu Leu Ala Leu Arg Gly Arg Glu Arg Pro Glu Leu Glu 195 200 205 <210> 432 <211> 208 <212> PRT <213> Bovine rhinitis <400> 432 Ser Gly Asn Pro Pro Thr Asp Met Gln Leu Val Val Leu Lys Asn Thr 1 5 10 15 Gln Pro Ile Ser Leu Pro Arg Asp Gly Gln Ile Val Ala Thr Cys Cys 20 25 30 Ala Leu Gly Val Phe Gly Thr Thr Tyr Leu Val Pro Tyr His Leu Phe 35 40 45 Glu Glu Ser Phe Asp Thr Leu Ile Ile Gly Asp Arg His Leu Lys Glu 50 55 60 Gln Asp Tyr Lys Leu Asp Thr Phe Glu Leu Arg Asp Gly Lys Ile Ser 65 70 75 80 Asp Val Ala Ala Leu Thr Leu Asn Lys Gly Ala Arg Val Arg Asp Ile 85 90 95 Thr Ser His Phe Arg Asp Glu Val Lys Val Thr Lys Asn Ser Pro Val 100 105 110 Val Gly Cys Val Lys Asn Thr Thr Val Gly Gln Leu Val Phe Asn Gly 115 120 125 Thr Ala Ala Gly Phe Lys Asp His Ile Ile Cys Ser Asp Gly Asp Thr 130 135 140 Leu Pro Asn Met Phe Val Tyr Thr Ala Asn Thr Gln Tyr Gly Tyr Cys 145 150 155 160 Gly Ser Gly Ile Leu Val Lys Asp Gly Ser His Thr Val Ile Ile Gly 165 170 175 Ile His Ser Ala Gly Gly Asn Gly Lys Gly Tyr Ala Ser Cys Val Thr 180 185 190 Arg Ser Ala Leu Leu Ala Leu Arg Gly Arg Glu Arg Pro Glu Leu Glu 195 200 205 <210> 433 <211> 624 <212> DNA <213> Bovine rhinitis <220> <221> CDS <222> (1)..(624) <220> <221> misc_feature <222> (1)..(624) <223> Bovine_Rhinitus_B_3C(wt) <400> 433 agt ggg aac cct cca act gac atg cag ttg gtg gta ctc aag aac aca 48 Ser Gly Asn Pro Pro Thr Asp Met Gln Leu Val Val Leu Lys Asn Thr 1 5 10 15 caa cca att tca cta gtt aga gat gga caa att gtc gcc act tgt tgt 96 Gln Pro Ile Ser Leu Val Arg Asp Gly Gln Ile Val Ala Thr Cys Cys 20 25 30 gct ctt ggc gtt ttt gga acc acc tat tta gtt ccc tac cac ctc ttt 144 Ala Leu Gly Val Phe Gly Thr Thr Tyr Leu Val Pro Tyr His Leu Phe 35 40 45 gaa gaa agt ttt gac acc ctc att ata gga gac aga cat ctg aaa gaa 192 Glu Glu Ser Phe Asp Thr Leu Ile Ile Gly Asp Arg His Leu Lys Glu 50 55 60 caa gac tac aaa ctt gac act ttt gaa ctc aga gac ggg aaa atc agc 240 Gln Asp Tyr Lys Leu Asp Thr Phe Glu Leu Arg Asp Gly Lys Ile Ser 65 70 75 80 gat gtt gct gcc ctc acc tta aac aaa gga gcc cgt gtg cgt gac att 288 Asp Val Ala Ala Leu Thr Leu Asn Lys Gly Ala Arg Val Arg Asp Ile 85 90 95 acc tca cac ttt cga gac gag gtc aag gtc act aag aat tca cca gtt 336 Thr Ser His Phe Arg Asp Glu Val Lys Val Thr Lys Asn Ser Pro Val 100 105 110 gtc ggt tgt gtc aag aac aca acg gtc ggt caa ctg gta ttt aac ggg 384 Val Gly Cys Val Lys Asn Thr Thr Val Gly Gln Leu Val Phe Asn Gly 115 120 125 aca gct gct ggt ttt aaa gac cat atc atc tgc tct gat ggt gac act 432 Thr Ala Ala Gly Phe Lys Asp His Ile Ile Cys Ser Asp Gly Asp Thr 130 135 140 ctg cct aat atg ttc gtt tac act gcc aac aca cag tac ggg tat tgc 480 Leu Pro Asn Met Phe Val Tyr Thr Ala Asn Thr Gln Tyr Gly Tyr Cys 145 150 155 160 gga agc ggc ata ctc gtc aaa gat ggt tct cac aca gtc atc ata ggc 528 Gly Ser Gly Ile Leu Val Lys Asp Gly Ser His Thr Val Ile Ile Gly 165 170 175 atc cat agt gca gga ggt aac gga aaa ggt tac gct agt tgt gtc act 576 Ile His Ser Ala Gly Gly Asn Gly Lys Gly Tyr Ala Ser Cys Val Thr 180 185 190 cgt tcc gca ctc ctc gca ctg agg ggc aga gaa agg ccc gag ctc gaa 624 Arg Ser Ala Leu Leu Ala Leu Arg Gly Arg Glu Arg Pro Glu Leu Glu 195 200 205 <210> 434 <211> 208 <212> PRT <213> Bovine rhinitis <400> 434 Ser Gly Asn Pro Pro Thr Asp Met Gln Leu Val Val Leu Lys Asn Thr 1 5 10 15 Gln Pro Ile Ser Leu Val Arg Asp Gly Gln Ile Val Ala Thr Cys Cys 20 25 30 Ala Leu Gly Val Phe Gly Thr Thr Tyr Leu Val Pro Tyr His Leu Phe 35 40 45 Glu Glu Ser Phe Asp Thr Leu Ile Ile Gly Asp Arg His Leu Lys Glu 50 55 60 Gln Asp Tyr Lys Leu Asp Thr Phe Glu Leu Arg Asp Gly Lys Ile Ser 65 70 75 80 Asp Val Ala Ala Leu Thr Leu Asn Lys Gly Ala Arg Val Arg Asp Ile 85 90 95 Thr Ser His Phe Arg Asp Glu Val Lys Val Thr Lys Asn Ser Pro Val 100 105 110 Val Gly Cys Val Lys Asn Thr Thr Val Gly Gln Leu Val Phe Asn Gly 115 120 125 Thr Ala Ala Gly Phe Lys Asp His Ile Ile Cys Ser Asp Gly Asp Thr 130 135 140 Leu Pro Asn Met Phe Val Tyr Thr Ala Asn Thr Gln Tyr Gly Tyr Cys 145 150 155 160 Gly Ser Gly Ile Leu Val Lys Asp Gly Ser His Thr Val Ile Ile Gly 165 170 175 Ile His Ser Ala Gly Gly Asn Gly Lys Gly Tyr Ala Ser Cys Val Thr 180 185 190 Arg Ser Ala Leu Leu Ala Leu Arg Gly Arg Glu Arg Pro Glu Leu Glu 195 200 205 <210> 435 <211> 615 <212> DNA <213> Equine Rhinitus <220> <221> CDS <222> (1)..(615) <220> <221> misc_feature <222> (1)..(615) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(615) <223> Equine_Rhinitus_A_3C(A125P) <400> 435 act gga gtt cca gca act gat ttg caa caa tct att atg aag aat gtt 48 Thr Gly Val Pro Ala Thr Asp Leu Gln Gln Ser Ile Met Lys Asn Val 1 5 10 15 cag cca att gag ctt tat tgt gat ggt aat ttg gtt act gat tgt tct 96 Gln Pro Ile Glu Leu Tyr Cys Asp Gly Asn Leu Val Thr Asp Cys Ser 20 25 30 gct ttg ggt gtt tat gat aac tcc tat ttg gtt ccc ttg cat ttg ttt 144 Ala Leu Gly Val Tyr Asp Asn Ser Tyr Leu Val Pro Leu His Leu Phe 35 40 45 gag ttt gat ttt gac acc att gtg ctt ggc ggt cgc cag tac aag aaa 192 Glu Phe Asp Phe Asp Thr Ile Val Leu Gly Gly Arg Gln Tyr Lys Lys 50 55 60 gct gat tgt gaa aaa gtg gaa ttt gaa ctt gaa att aat gga gat atg 240 Ala Asp Cys Glu Lys Val Glu Phe Glu Leu Glu Ile Asn Gly Asp Met 65 70 75 80 gtg tca tcg gac gcg tgc ttg ctc cgg gtt cca acc gga cca aaa gtg 288 Val Ser Ser Asp Ala Cys Leu Leu Arg Val Pro Thr Gly Pro Lys Val 85 90 95 aga aat att gtt cat ctt ttc aca aat gaa att gag tta aaa aag atg 336 Arg Asn Ile Val His Leu Phe Thr Asn Glu Ile Glu Leu Lys Lys Met 100 105 110 acc caa att aca gga att atg aat tct cca cac caa ccn cgc act gtg 384 Thr Gln Ile Thr Gly Ile Met Asn Ser Pro His Gln Pro Arg Thr Val 115 120 125 ttt ttt ggc agt ttt ttg aca gta aaa aaa tct att ttg aca tca gat 432 Phe Phe Gly Ser Phe Leu Thr Val Lys Lys Ser Ile Leu Thr Ser Asp 130 135 140 ggg act gtt atg cct aat gtc ttg tct tac gca gcc cag acc tcg cgg 480 Gly Thr Val Met Pro Asn Val Leu Ser Tyr Ala Ala Gln Thr Ser Arg 145 150 155 160 ggg tat tgt ggc gca gca att gtg gca ggc tca cct gcc cgc atc att 528 Gly Tyr Cys Gly Ala Ala Ile Val Ala Gly Ser Pro Ala Arg Ile Ile 165 170 175 ggc att cat tct gct ggt act ggg tct gta gcc ttt tgc tcc ctg gtg 576 Gly Ile His Ser Ala Gly Thr Gly Ser Val Ala Phe Cys Ser Leu Val 180 185 190 tcc aga gat gcg ctg gag cgc act ctc cct cag aaa cag 615 Ser Arg Asp Ala Leu Glu Arg Thr Leu Pro Gln Lys Gln 195 200 205 <210> 436 <211> 205 <212> PRT <213> Equine Rhinitus <400> 436 Thr Gly Val Pro Ala Thr Asp Leu Gln Gln Ser Ile Met Lys Asn Val 1 5 10 15 Gln Pro Ile Glu Leu Tyr Cys Asp Gly Asn Leu Val Thr Asp Cys Ser 20 25 30 Ala Leu Gly Val Tyr Asp Asn Ser Tyr Leu Val Pro Leu His Leu Phe 35 40 45 Glu Phe Asp Phe Asp Thr Ile Val Leu Gly Gly Arg Gln Tyr Lys Lys 50 55 60 Ala Asp Cys Glu Lys Val Glu Phe Glu Leu Glu Ile Asn Gly Asp Met 65 70 75 80 Val Ser Ser Asp Ala Cys Leu Leu Arg Val Pro Thr Gly Pro Lys Val 85 90 95 Arg Asn Ile Val His Leu Phe Thr Asn Glu Ile Glu Leu Lys Lys Met 100 105 110 Thr Gln Ile Thr Gly Ile Met Asn Ser Pro His Gln Pro Arg Thr Val 115 120 125 Phe Phe Gly Ser Phe Leu Thr Val Lys Lys Ser Ile Leu Thr Ser Asp 130 135 140 Gly Thr Val Met Pro Asn Val Leu Ser Tyr Ala Ala Gln Thr Ser Arg 145 150 155 160 Gly Tyr Cys Gly Ala Ala Ile Val Ala Gly Ser Pro Ala Arg Ile Ile 165 170 175 Gly Ile His Ser Ala Gly Thr Gly Ser Val Ala Phe Cys Ser Leu Val 180 185 190 Ser Arg Asp Ala Leu Glu Arg Thr Leu Pro Gln Lys Gln 195 200 205 <210> 437 <211> 615 <212> DNA <213> Equine rhinitis <220> <221> CDS <222> (1)..(615) <220> <221> misc_feature <222> (1)..(615) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(615) <223> Equine_Rhinitus_A_3C(C23P) <400> 437 act gga gtt cca gca act gat ttg caa caa tct att atg aag aat gtt 48 Thr Gly Val Pro Ala Thr Asp Leu Gln Gln Ser Ile Met Lys Asn Val 1 5 10 15 cag cca att gag ctt tat ccn gat ggt aat ttg gtt act gat tgt tct 96 Gln Pro Ile Glu Leu Tyr Pro Asp Gly Asn Leu Val Thr Asp Cys Ser 20 25 30 gct ttg ggt gtt tat gat aac tcc tat ttg gtt ccc ttg cat ttg ttt 144 Ala Leu Gly Val Tyr Asp Asn Ser Tyr Leu Val Pro Leu His Leu Phe 35 40 45 gag ttt gat ttt gac acc att gtg ctt ggc ggt cgc cag tac aag aaa 192 Glu Phe Asp Phe Asp Thr Ile Val Leu Gly Gly Arg Gln Tyr Lys Lys 50 55 60 gct gat tgt gaa aaa gtg gaa ttt gaa ctt gaa att aat gga gat atg 240 Ala Asp Cys Glu Lys Val Glu Phe Glu Leu Glu Ile Asn Gly Asp Met 65 70 75 80 gtg tca tcg gac gcg tgc ttg ctc cgg gtt cca acc gga cca aaa gtg 288 Val Ser Ser Asp Ala Cys Leu Leu Arg Val Pro Thr Gly Pro Lys Val 85 90 95 aga aat att gtt cat ctt ttc aca aat gaa att gag tta aaa aag atg 336 Arg Asn Ile Val His Leu Phe Thr Asn Glu Ile Glu Leu Lys Lys Met 100 105 110 acc caa att aca gga att atg aat tct cca cac caa gca cgc act gtg 384 Thr Gln Ile Thr Gly Ile Met Asn Ser Pro His Gln Ala Arg Thr Val 115 120 125 ttt ttt ggc agt ttt ttg aca gta aaa aaa tct att ttg aca tca gat 432 Phe Phe Gly Ser Phe Leu Thr Val Lys Lys Ser Ile Leu Thr Ser Asp 130 135 140 ggg act gtt atg cct aat gtc ttg tct tac gca gcc cag acc tcg cgg 480 Gly Thr Val Met Pro Asn Val Leu Ser Tyr Ala Ala Gln Thr Ser Arg 145 150 155 160 ggg tat tgt ggc gca gca att gtg gca ggc tca cct gcc cgc atc att 528 Gly Tyr Cys Gly Ala Ala Ile Val Ala Gly Ser Pro Ala Arg Ile Ile 165 170 175 ggc att cat tct gct ggt act ggg tct gta gcc ttt tgc tcc ctg gtg 576 Gly Ile His Ser Ala Gly Thr Gly Ser Val Ala Phe Cys Ser Leu Val 180 185 190 tcc aga gat gcg ctg gag cgc act ctc cct cag aaa cag 615 Ser Arg Asp Ala Leu Glu Arg Thr Leu Pro Gln Lys Gln 195 200 205 <210> 438 <211> 205 <212> PRT <213> Equine rhinitis <400> 438 Thr Gly Val Pro Ala Thr Asp Leu Gln Gln Ser Ile Met Lys Asn Val 1 5 10 15 Gln Pro Ile Glu Leu Tyr Pro Asp Gly Asn Leu Val Thr Asp Cys Ser 20 25 30 Ala Leu Gly Val Tyr Asp Asn Ser Tyr Leu Val Pro Leu His Leu Phe 35 40 45 Glu Phe Asp Phe Asp Thr Ile Val Leu Gly Gly Arg Gln Tyr Lys Lys 50 55 60 Ala Asp Cys Glu Lys Val Glu Phe Glu Leu Glu Ile Asn Gly Asp Met 65 70 75 80 Val Ser Ser Asp Ala Cys Leu Leu Arg Val Pro Thr Gly Pro Lys Val 85 90 95 Arg Asn Ile Val His Leu Phe Thr Asn Glu Ile Glu Leu Lys Lys Met 100 105 110 Thr Gln Ile Thr Gly Ile Met Asn Ser Pro His Gln Ala Arg Thr Val 115 120 125 Phe Phe Gly Ser Phe Leu Thr Val Lys Lys Ser Ile Leu Thr Ser Asp 130 135 140 Gly Thr Val Met Pro Asn Val Leu Ser Tyr Ala Ala Gln Thr Ser Arg 145 150 155 160 Gly Tyr Cys Gly Ala Ala Ile Val Ala Gly Ser Pro Ala Arg Ile Ile 165 170 175 Gly Ile His Ser Ala Gly Thr Gly Ser Val Ala Phe Cys Ser Leu Val 180 185 190 Ser Arg Asp Ala Leu Glu Arg Thr Leu Pro Gln Lys Gln 195 200 205 <210> 439 <211> 615 <212> DNA <213> Equine Rhinitus <220> <221> CDS <222> (1)..(615) <220> <221> misc_feature <222> (1)..(615) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(615) <223> Equine_Rhinitus_A_3C(T127P) <400> 439 act gga gtt cca gca act gat ttg caa caa tct att atg aag aat gtt 48 Thr Gly Val Pro Ala Thr Asp Leu Gln Gln Ser Ile Met Lys Asn Val 1 5 10 15 cag cca att gag ctt tat tgt gat ggt aat ttg gtt act gat tgt tct 96 Gln Pro Ile Glu Leu Tyr Cys Asp Gly Asn Leu Val Thr Asp Cys Ser 20 25 30 gct ttg ggt gtt tat gat aac tcc tat ttg gtt ccc ttg cat ttg ttt 144 Ala Leu Gly Val Tyr Asp Asn Ser Tyr Leu Val Pro Leu His Leu Phe 35 40 45 gag ttt gat ttt gac acc att gtg ctt ggc ggt cgc cag tac aag aaa 192 Glu Phe Asp Phe Asp Thr Ile Val Leu Gly Gly Arg Gln Tyr Lys Lys 50 55 60 gct gat tgt gaa aaa gtg gaa ttt gaa ctt gaa att aat gga gat atg 240 Ala Asp Cys Glu Lys Val Glu Phe Glu Leu Glu Ile Asn Gly Asp Met 65 70 75 80 gtg tca tcg gac gcg tgc ttg ctc cgg gtt cca acc gga cca aaa gtg 288 Val Ser Ser Asp Ala Cys Leu Leu Arg Val Pro Thr Gly Pro Lys Val 85 90 95 aga aat att gtt cat ctt ttc aca aat gaa att gag tta aaa aag atg 336 Arg Asn Ile Val His Leu Phe Thr Asn Glu Ile Glu Leu Lys Lys Met 100 105 110 acc caa att aca gga att atg aat tct cca cac caa gca cgc ccn gtg 384 Thr Gln Ile Thr Gly Ile Met Asn Ser Pro His Gln Ala Arg Pro Val 115 120 125 ttt ttt ggc agt ttt ttg aca gta aaa aaa tct att ttg aca tca gat 432 Phe Phe Gly Ser Phe Leu Thr Val Lys Lys Ser Ile Leu Thr Ser Asp 130 135 140 ggg act gtt atg cct aat gtc ttg tct tac gca gcc cag acc tcg cgg 480 Gly Thr Val Met Pro Asn Val Leu Ser Tyr Ala Ala Gln Thr Ser Arg 145 150 155 160 ggg tat tgt ggc gca gca att gtg gca ggc tca cct gcc cgc atc att 528 Gly Tyr Cys Gly Ala Ala Ile Val Ala Gly Ser Pro Ala Arg Ile Ile 165 170 175 ggc att cat tct gct ggt act ggg tct gta gcc ttt tgc tcc ctg gtg 576 Gly Ile His Ser Ala Gly Thr Gly Ser Val Ala Phe Cys Ser Leu Val 180 185 190 tcc aga gat gcg ctg gag cgc act ctc cct cag aaa cag 615 Ser Arg Asp Ala Leu Glu Arg Thr Leu Pro Gln Lys Gln 195 200 205 <210> 440 <211> 205 <212> PRT <213> Equine Rhinitus <400> 440 Thr Gly Val Pro Ala Thr Asp Leu Gln Gln Ser Ile Met Lys Asn Val 1 5 10 15 Gln Pro Ile Glu Leu Tyr Cys Asp Gly Asn Leu Val Thr Asp Cys Ser 20 25 30 Ala Leu Gly Val Tyr Asp Asn Ser Tyr Leu Val Pro Leu His Leu Phe 35 40 45 Glu Phe Asp Phe Asp Thr Ile Val Leu Gly Gly Arg Gln Tyr Lys Lys 50 55 60 Ala Asp Cys Glu Lys Val Glu Phe Glu Leu Glu Ile Asn Gly Asp Met 65 70 75 80 Val Ser Ser Asp Ala Cys Leu Leu Arg Val Pro Thr Gly Pro Lys Val 85 90 95 Arg Asn Ile Val His Leu Phe Thr Asn Glu Ile Glu Leu Lys Lys Met 100 105 110 Thr Gln Ile Thr Gly Ile Met Asn Ser Pro His Gln Ala Arg Pro Val 115 120 125 Phe Phe Gly Ser Phe Leu Thr Val Lys Lys Ser Ile Leu Thr Ser Asp 130 135 140 Gly Thr Val Met Pro Asn Val Leu Ser Tyr Ala Ala Gln Thr Ser Arg 145 150 155 160 Gly Tyr Cys Gly Ala Ala Ile Val Ala Gly Ser Pro Ala Arg Ile Ile 165 170 175 Gly Ile His Ser Ala Gly Thr Gly Ser Val Ala Phe Cys Ser Leu Val 180 185 190 Ser Arg Asp Ala Leu Glu Arg Thr Leu Pro Gln Lys Gln 195 200 205 <210> 441 <211> 615 <212> DNA <213> Equine Rhinitus <220> <221> CDS <222> (1)..(615) <220> <221> misc_feature <222> (1)..(615) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(615) <223> Equine_Rhinitus_A_3C(V100P) <400> 441 act gga gtt cca gca act gat ttg caa caa tct att atg aag aat gtt 48 Thr Gly Val Pro Ala Thr Asp Leu Gln Gln Ser Ile Met Lys Asn Val 1 5 10 15 cag cca att gag ctt tat tgt gat ggt aat ttg gtt act gat tgt tct 96 Gln Pro Ile Glu Leu Tyr Cys Asp Gly Asn Leu Val Thr Asp Cys Ser 20 25 30 gct ttg ggt gtt tat gat aac tcc tat ttg gtt ccc ttg cat ttg ttt 144 Ala Leu Gly Val Tyr Asp Asn Ser Tyr Leu Val Pro Leu His Leu Phe 35 40 45 gag ttt gat ttt gac acc att gtg ctt ggc ggt cgc cag tac aag aaa 192 Glu Phe Asp Phe Asp Thr Ile Val Leu Gly Gly Arg Gln Tyr Lys Lys 50 55 60 gct gat tgt gaa aaa gtg gaa ttt gaa ctt gaa att aat gga gat atg 240 Ala Asp Cys Glu Lys Val Glu Phe Glu Leu Glu Ile Asn Gly Asp Met 65 70 75 80 gtg tca tcg gac gcg tgc ttg ctc cgg gtt cca acc gga cca aaa gtg 288 Val Ser Ser Asp Ala Cys Leu Leu Arg Val Pro Thr Gly Pro Lys Val 85 90 95 aga aat att ccn cat ctt ttc aca aat gaa att gag tta aaa aag atg 336 Arg Asn Ile Pro His Leu Phe Thr Asn Glu Ile Glu Leu Lys Lys Met 100 105 110 acc caa att aca gga att atg aat tct cca cac caa gca cgc act gtg 384 Thr Gln Ile Thr Gly Ile Met Asn Ser Pro His Gln Ala Arg Thr Val 115 120 125 ttt ttt ggc agt ttt ttg aca gta aaa aaa tct att ttg aca tca gat 432 Phe Phe Gly Ser Phe Leu Thr Val Lys Lys Ser Ile Leu Thr Ser Asp 130 135 140 ggg act gtt atg cct aat gtc ttg tct tac gca gcc cag acc tcg cgg 480 Gly Thr Val Met Pro Asn Val Leu Ser Tyr Ala Ala Gln Thr Ser Arg 145 150 155 160 ggg tat tgt ggc gca gca att gtg gca ggc tca cct gcc cgc atc att 528 Gly Tyr Cys Gly Ala Ala Ile Val Ala Gly Ser Pro Ala Arg Ile Ile 165 170 175 ggc att cat tct gct ggt act ggg tct gta gcc ttt tgc tcc ctg gtg 576 Gly Ile His Ser Ala Gly Thr Gly Ser Val Ala Phe Cys Ser Leu Val 180 185 190 tcc aga gat gcg ctg gag cgc act ctc cct cag aaa cag 615 Ser Arg Asp Ala Leu Glu Arg Thr Leu Pro Gln Lys Gln 195 200 205 <210> 442 <211> 205 <212> PRT <213> Equine Rhinitus <400> 442 Thr Gly Val Pro Ala Thr Asp Leu Gln Gln Ser Ile Met Lys Asn Val 1 5 10 15 Gln Pro Ile Glu Leu Tyr Cys Asp Gly Asn Leu Val Thr Asp Cys Ser 20 25 30 Ala Leu Gly Val Tyr Asp Asn Ser Tyr Leu Val Pro Leu His Leu Phe 35 40 45 Glu Phe Asp Phe Asp Thr Ile Val Leu Gly Gly Arg Gln Tyr Lys Lys 50 55 60 Ala Asp Cys Glu Lys Val Glu Phe Glu Leu Glu Ile Asn Gly Asp Met 65 70 75 80 Val Ser Ser Asp Ala Cys Leu Leu Arg Val Pro Thr Gly Pro Lys Val 85 90 95 Arg Asn Ile Pro His Leu Phe Thr Asn Glu Ile Glu Leu Lys Lys Met 100 105 110 Thr Gln Ile Thr Gly Ile Met Asn Ser Pro His Gln Ala Arg Thr Val 115 120 125 Phe Phe Gly Ser Phe Leu Thr Val Lys Lys Ser Ile Leu Thr Ser Asp 130 135 140 Gly Thr Val Met Pro Asn Val Leu Ser Tyr Ala Ala Gln Thr Ser Arg 145 150 155 160 Gly Tyr Cys Gly Ala Ala Ile Val Ala Gly Ser Pro Ala Arg Ile Ile 165 170 175 Gly Ile His Ser Ala Gly Thr Gly Ser Val Ala Phe Cys Ser Leu Val 180 185 190 Ser Arg Asp Ala Leu Glu Arg Thr Leu Pro Gln Lys Gln 195 200 205 <210> 443 <211> 615 <212> DNA <213> Equine Rhinitus <220> <221> CDS <222> (1)..(615) <220> <221> misc_feature <222> (1)..(615) <223> Equine_Rhinitus_A_3C(wt) <400> 443 act gga gtt cca gca act gat ttg caa caa tct att atg aag aat gtt 48 Thr Gly Val Pro Ala Thr Asp Leu Gln Gln Ser Ile Met Lys Asn Val 1 5 10 15 cag cca att gag ctt tat tgt gat ggt aat ttg gtt act gat tgt tct 96 Gln Pro Ile Glu Leu Tyr Cys Asp Gly Asn Leu Val Thr Asp Cys Ser 20 25 30 gct ttg ggt gtt tat gat aac tcc tat ttg gtt ccc ttg cat ttg ttt 144 Ala Leu Gly Val Tyr Asp Asn Ser Tyr Leu Val Pro Leu His Leu Phe 35 40 45 gag ttt gat ttt gac acc att gtg ctt ggc ggt cgc cag tac aag aaa 192 Glu Phe Asp Phe Asp Thr Ile Val Leu Gly Gly Arg Gln Tyr Lys Lys 50 55 60 gct gat tgt gaa aaa gtg gaa ttt gaa ctt gaa att aat gga gat atg 240 Ala Asp Cys Glu Lys Val Glu Phe Glu Leu Glu Ile Asn Gly Asp Met 65 70 75 80 gtg tca tcg gac gcg tgc ttg ctc cgg gtt cca acc gga cca aaa gtg 288 Val Ser Ser Asp Ala Cys Leu Leu Arg Val Pro Thr Gly Pro Lys Val 85 90 95 aga aat att gtt cat ctt ttc aca aat gaa att gag tta aaa aag atg 336 Arg Asn Ile Val His Leu Phe Thr Asn Glu Ile Glu Leu Lys Lys Met 100 105 110 acc caa att aca gga att atg aat tct cca cac caa gca cgc act gtg 384 Thr Gln Ile Thr Gly Ile Met Asn Ser Pro His Gln Ala Arg Thr Val 115 120 125 ttt ttt ggc agt ttt ttg aca gta aaa aaa tct att ttg aca tca gat 432 Phe Phe Gly Ser Phe Leu Thr Val Lys Lys Ser Ile Leu Thr Ser Asp 130 135 140 ggg act gtt atg cct aat gtc ttg tct tac gca gcc cag acc tcg cgg 480 Gly Thr Val Met Pro Asn Val Leu Ser Tyr Ala Ala Gln Thr Ser Arg 145 150 155 160 ggg tat tgt ggc gca gca att gtg gca ggc tca cct gcc cgc atc att 528 Gly Tyr Cys Gly Ala Ala Ile Val Ala Gly Ser Pro Ala Arg Ile Ile 165 170 175 ggc att cat tct gct ggt act ggg tct gta gcc ttt tgc tcc ctg gtg 576 Gly Ile His Ser Ala Gly Thr Gly Ser Val Ala Phe Cys Ser Leu Val 180 185 190 tcc aga gat gcg ctg gag cgc act ctc cct cag aaa cag 615 Ser Arg Asp Ala Leu Glu Arg Thr Leu Pro Gln Lys Gln 195 200 205 <210> 444 <211> 205 <212> PRT <213> Equine Rhinitus <400> 444 Thr Gly Val Pro Ala Thr Asp Leu Gln Gln Ser Ile Met Lys Asn Val 1 5 10 15 Gln Pro Ile Glu Leu Tyr Cys Asp Gly Asn Leu Val Thr Asp Cys Ser 20 25 30 Ala Leu Gly Val Tyr Asp Asn Ser Tyr Leu Val Pro Leu His Leu Phe 35 40 45 Glu Phe Asp Phe Asp Thr Ile Val Leu Gly Gly Arg Gln Tyr Lys Lys 50 55 60 Ala Asp Cys Glu Lys Val Glu Phe Glu Leu Glu Ile Asn Gly Asp Met 65 70 75 80 Val Ser Ser Asp Ala Cys Leu Leu Arg Val Pro Thr Gly Pro Lys Val 85 90 95 Arg Asn Ile Val His Leu Phe Thr Asn Glu Ile Glu Leu Lys Lys Met 100 105 110 Thr Gln Ile Thr Gly Ile Met Asn Ser Pro His Gln Ala Arg Thr Val 115 120 125 Phe Phe Gly Ser Phe Leu Thr Val Lys Lys Ser Ile Leu Thr Ser Asp 130 135 140 Gly Thr Val Met Pro Asn Val Leu Ser Tyr Ala Ala Gln Thr Ser Arg 145 150 155 160 Gly Tyr Cys Gly Ala Ala Ile Val Ala Gly Ser Pro Ala Arg Ile Ile 165 170 175 Gly Ile His Ser Ala Gly Thr Gly Ser Val Ala Phe Cys Ser Leu Val 180 185 190 Ser Arg Asp Ala Leu Glu Arg Thr Leu Pro Gln Lys Gln 195 200 205 <210> 445 <211> 615 <212> DNA <213> Equine Rhinitus <220> <221> CDS <222> (1)..(615) <220> <221> misc_feature <222> (1)..(615) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(615) <223> Equine_Rhinitus_A_3C(Y22P) <400> 445 act gga gtt cca gca act gat ttg caa caa tct att atg aag aat gtt 48 Thr Gly Val Pro Ala Thr Asp Leu Gln Gln Ser Ile Met Lys Asn Val 1 5 10 15 cag cca att gag ctt ccn tgt gat ggt aat ttg gtt act gat tgt tct 96 Gln Pro Ile Glu Leu Pro Cys Asp Gly Asn Leu Val Thr Asp Cys Ser 20 25 30 gct ttg ggt gtt tat gat aac tcc tat ttg gtt ccc ttg cat ttg ttt 144 Ala Leu Gly Val Tyr Asp Asn Ser Tyr Leu Val Pro Leu His Leu Phe 35 40 45 gag ttt gat ttt gac acc att gtg ctt ggc ggt cgc cag tac aag aaa 192 Glu Phe Asp Phe Asp Thr Ile Val Leu Gly Gly Arg Gln Tyr Lys Lys 50 55 60 gct gat tgt gaa aaa gtg gaa ttt gaa ctt gaa att aat gga gat atg 240 Ala Asp Cys Glu Lys Val Glu Phe Glu Leu Glu Ile Asn Gly Asp Met 65 70 75 80 gtg tca tcg gac gcg tgc ttg ctc cgg gtt cca acc gga cca aaa gtg 288 Val Ser Ser Asp Ala Cys Leu Leu Arg Val Pro Thr Gly Pro Lys Val 85 90 95 aga aat att gtt cat ctt ttc aca aat gaa att gag tta aaa aag atg 336 Arg Asn Ile Val His Leu Phe Thr Asn Glu Ile Glu Leu Lys Lys Met 100 105 110 acc caa att aca gga att atg aat tct cca cac caa gca cgc act gtg 384 Thr Gln Ile Thr Gly Ile Met Asn Ser Pro His Gln Ala Arg Thr Val 115 120 125 ttt ttt ggc agt ttt ttg aca gta aaa aaa tct att ttg aca tca gat 432 Phe Phe Gly Ser Phe Leu Thr Val Lys Lys Ser Ile Leu Thr Ser Asp 130 135 140 ggg act gtt atg cct aat gtc ttg tct tac gca gcc cag acc tcg cgg 480 Gly Thr Val Met Pro Asn Val Leu Ser Tyr Ala Ala Gln Thr Ser Arg 145 150 155 160 ggg tat tgt ggc gca gca att gtg gca ggc tca cct gcc cgc atc att 528 Gly Tyr Cys Gly Ala Ala Ile Val Ala Gly Ser Pro Ala Arg Ile Ile 165 170 175 ggc att cat tct gct ggt act ggg tct gta gcc ttt tgc tcc ctg gtg 576 Gly Ile His Ser Ala Gly Thr Gly Ser Val Ala Phe Cys Ser Leu Val 180 185 190 tcc aga gat gcg ctg gag cgc act ctc cct cag aaa cag 615 Ser Arg Asp Ala Leu Glu Arg Thr Leu Pro Gln Lys Gln 195 200 205 <210> 446 <211> 205 <212> PRT <213> Equine Rhinitus <400> 446 Thr Gly Val Pro Ala Thr Asp Leu Gln Gln Ser Ile Met Lys Asn Val 1 5 10 15 Gln Pro Ile Glu Leu Pro Cys Asp Gly Asn Leu Val Thr Asp Cys Ser 20 25 30 Ala Leu Gly Val Tyr Asp Asn Ser Tyr Leu Val Pro Leu His Leu Phe 35 40 45 Glu Phe Asp Phe Asp Thr Ile Val Leu Gly Gly Arg Gln Tyr Lys Lys 50 55 60 Ala Asp Cys Glu Lys Val Glu Phe Glu Leu Glu Ile Asn Gly Asp Met 65 70 75 80 Val Ser Ser Asp Ala Cys Leu Leu Arg Val Pro Thr Gly Pro Lys Val 85 90 95 Arg Asn Ile Val His Leu Phe Thr Asn Glu Ile Glu Leu Lys Lys Met 100 105 110 Thr Gln Ile Thr Gly Ile Met Asn Ser Pro His Gln Ala Arg Thr Val 115 120 125 Phe Phe Gly Ser Phe Leu Thr Val Lys Lys Ser Ile Leu Thr Ser Asp 130 135 140 Gly Thr Val Met Pro Asn Val Leu Ser Tyr Ala Ala Gln Thr Ser Arg 145 150 155 160 Gly Tyr Cys Gly Ala Ala Ile Val Ala Gly Ser Pro Ala Arg Ile Ile 165 170 175 Gly Ile His Ser Ala Gly Thr Gly Ser Val Ala Phe Cys Ser Leu Val 180 185 190 Ser Arg Asp Ala Leu Glu Arg Thr Leu Pro Gln Lys Gln 195 200 205 <210> 447 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A1_Tompkins_3C(F109P) <400> 447 ggc cca gta ttt gat tat gca gtt gca atg gca aag aaa aac atc ctg 48 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 aca gcc acc aca gaa aag ggt gaa ttc aca atg tta gga gta cat gat 96 Thr Ala Thr Thr Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aga gtt gca gta ttg cct acc cat tca aac cca ggt gaa aca ata gtt 144 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 gtg tca gga aaa gaa gtg aag atc ttg gat gca aag agg ctg gtt gat 192 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 aat gat gag gtc aat ctt gaa att act att gta acc cta gac aga aac 240 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 gaa aag ttt aga gac atc aga act cac tta ccg acc caa atc cat gag 288 Glu Lys Phe Arg Asp Ile Arg Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 acc aat gat gcg gtt ttg gct gtc aac acc tcc aaa ccn cca aat atg 336 Thr Asn Asp Ala Val Leu Ala Val Asn Thr Ser Lys Pro Pro Asn Met 100 105 110 tac att cca gtt gga tca gta gtt gaa cag gga atg ttg aac ctt ggt 384 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 ggc aga ccc aca aat aga acc cta atg tat aac ttt cca act aaa gca 432 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 ggg cag tgt ggt ggt gtc tta atg gcc act ggc aaa gtg att ggc ata 480 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 cat gtt ggt ggt aat gga tct cac ggt ttc gca gct gca ctt aaa aga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 agt tac ttt act gaa gaa cag 549 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 448 <211> 183 <212> PRT <213> Human coxsackievirus <400> 448 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 Thr Ala Thr Thr Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 Thr Asn Asp Ala Val Leu Ala Val Asn Thr Ser Lys Pro Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 449 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A1_Tompkins_3C(L102P) <400> 449 ggc cca gta ttt gat tat gca gtt gca atg gca aag aaa aac atc ctg 48 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 aca gcc acc aca gaa aag ggt gaa ttc aca atg tta gga gta cat gat 96 Thr Ala Thr Thr Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aga gtt gca gta ttg cct acc cat tca aac cca ggt gaa aca ata gtt 144 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 gtg tca gga aaa gaa gtg aag atc ttg gat gca aag agg ctg gtt gat 192 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 aat gat gag gtc aat ctt gaa att act att gta acc cta gac aga aac 240 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 gaa aag ttt aga gac atc aga act cac tta ccg acc caa atc cat gag 288 Glu Lys Phe Arg Asp Ile Arg Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 acc aat gat gcg gtt ccn gct gtc aac acc tcc aaa ttt cca aat atg 336 Thr Asn Asp Ala Val Pro Ala Val Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tac att cca gtt gga tca gta gtt gaa cag gga atg ttg aac ctt ggt 384 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 ggc aga ccc aca aat aga acc cta atg tat aac ttt cca act aaa gca 432 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 ggg cag tgt ggt ggt gtc tta atg gcc act ggc aaa gtg att ggc ata 480 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 cat gtt ggt ggt aat gga tct cac ggt ttc gca gct gca ctt aaa aga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 agt tac ttt act gaa gaa cag 549 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 450 <211> 183 <212> PRT <213> Human coxsackievirus <400> 450 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 Thr Ala Thr Thr Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 Thr Asn Asp Ala Val Pro Ala Val Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 451 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A1_Tompkins_3C(N105P) <400> 451 ggc cca gta ttt gat tat gca gtt gca atg gca aag aaa aac atc ctg 48 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 aca gcc acc aca gaa aag ggt gaa ttc aca atg tta gga gta cat gat 96 Thr Ala Thr Thr Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aga gtt gca gta ttg cct acc cat tca aac cca ggt gaa aca ata gtt 144 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 gtg tca gga aaa gaa gtg aag atc ttg gat gca aag agg ctg gtt gat 192 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 aat gat gag gtc aat ctt gaa att act att gta acc cta gac aga aac 240 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 gaa aag ttt aga gac atc aga act cac tta ccg acc caa atc cat gag 288 Glu Lys Phe Arg Asp Ile Arg Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 acc aat gat gcg gtt ttg gct gtc ccn acc tcc aaa ttt cca aat atg 336 Thr Asn Asp Ala Val Leu Ala Val Pro Thr Ser Lys Phe Pro Asn Met 100 105 110 tac att cca gtt gga tca gta gtt gaa cag gga atg ttg aac ctt ggt 384 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 ggc aga ccc aca aat aga acc cta atg tat aac ttt cca act aaa gca 432 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 ggg cag tgt ggt ggt gtc tta atg gcc act ggc aaa gtg att ggc ata 480 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 cat gtt ggt ggt aat gga tct cac ggt ttc gca gct gca ctt aaa aga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 agt tac ttt act gaa gaa cag 549 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 452 <211> 183 <212> PRT <213> Human coxsackievirus <400> 452 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 Thr Ala Thr Thr Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 Thr Asn Asp Ala Val Leu Ala Val Pro Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 453 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A1_Tompkins_3C(R87P) <400> 453 ggc cca gta ttt gat tat gca gtt gca atg gca aag aaa aac atc ctg 48 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 aca gcc acc aca gaa aag ggt gaa ttc aca atg tta gga gta cat gat 96 Thr Ala Thr Thr Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aga gtt gca gta ttg cct acc cat tca aac cca ggt gaa aca ata gtt 144 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 gtg tca gga aaa gaa gtg aag atc ttg gat gca aag agg ctg gtt gat 192 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 aat gat gag gtc aat ctt gaa att act att gta acc cta gac aga aac 240 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 gaa aag ttt aga gac atc ccn act cac tta ccg acc caa atc cat gag 288 Glu Lys Phe Arg Asp Ile Pro Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 acc aat gat gcg gtt ttg gct gtc aac acc tcc aaa ttt cca aat atg 336 Thr Asn Asp Ala Val Leu Ala Val Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tac att cca gtt gga tca gta gtt gaa cag gga atg ttg aac ctt ggt 384 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 ggc aga ccc aca aat aga acc cta atg tat aac ttt cca act aaa gca 432 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 ggg cag tgt ggt ggt gtc tta atg gcc act ggc aaa gtg att ggc ata 480 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 cat gtt ggt ggt aat gga tct cac ggt ttc gca gct gca ctt aaa aga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 agt tac ttt act gaa gaa cag 549 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 454 <211> 183 <212> PRT <213> Human coxsackievirus <400> 454 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 Thr Ala Thr Thr Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Pro Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 Thr Asn Asp Ala Val Leu Ala Val Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 455 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A1_Tompkins_3C(T106P) <400> 455 ggc cca gta ttt gat tat gca gtt gca atg gca aag aaa aac atc ctg 48 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 aca gcc acc aca gaa aag ggt gaa ttc aca atg tta gga gta cat gat 96 Thr Ala Thr Thr Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aga gtt gca gta ttg cct acc cat tca aac cca ggt gaa aca ata gtt 144 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 gtg tca gga aaa gaa gtg aag atc ttg gat gca aag agg ctg gtt gat 192 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 aat gat gag gtc aat ctt gaa att act att gta acc cta gac aga aac 240 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 gaa aag ttt aga gac atc aga act cac tta ccg acc caa atc cat gag 288 Glu Lys Phe Arg Asp Ile Arg Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 acc aat gat gcg gtt ttg gct gtc aac ccn tcc aaa ttt cca aat atg 336 Thr Asn Asp Ala Val Leu Ala Val Asn Pro Ser Lys Phe Pro Asn Met 100 105 110 tac att cca gtt gga tca gta gtt gaa cag gga atg ttg aac ctt ggt 384 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 ggc aga ccc aca aat aga acc cta atg tat aac ttt cca act aaa gca 432 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 ggg cag tgt ggt ggt gtc tta atg gcc act ggc aaa gtg att ggc ata 480 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 cat gtt ggt ggt aat gga tct cac ggt ttc gca gct gca ctt aaa aga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 agt tac ttt act gaa gaa cag 549 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 456 <211> 183 <212> PRT <213> Human coxsackievirus <400> 456 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 Thr Ala Thr Thr Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 Thr Asn Asp Ala Val Leu Ala Val Asn Pro Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 457 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A1_Tompkins_3C(T19P) <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A1_Tompkins_3C(T19P) <400> 457 ggc cca gta ttt gat tat gca gtt gca atg gca aag aaa aac atc ctg 48 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 aca gcc ccn aca gaa aag ggt gaa ttc aca atg tta gga gta cat gat 96 Thr Ala Pro Thr Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aga gtt gca gta ttg cct acc cat tca aac cca ggt gaa aca ata gtt 144 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 gtg tca gga aaa gaa gtg aag atc ttg gat gca aag agg ctg gtt gat 192 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 aat gat gag gtc aat ctt gaa att act att gta acc cta gac aga aac 240 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 gaa aag ttt aga gac atc aga act cac tta ccg acc caa atc cat gag 288 Glu Lys Phe Arg Asp Ile Arg Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 acc aat gat gcg gtt ttg gct gtc aac acc tcc aaa ttt cca aat atg 336 Thr Asn Asp Ala Val Leu Ala Val Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tac att cca gtt gga tca gta gtt gaa cag gga atg ttg aac ctt ggt 384 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 ggc aga ccc aca aat aga acc cta atg tat aac ttt cca act aaa gca 432 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 ggg cag tgt ggt ggt gtc tta atg gcc act ggc aaa gtg att ggc ata 480 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 cat gtt ggt ggt aat gga tct cac ggt ttc gca gct gca ctt aaa aga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 agt tac ttt act gaa gaa cag 549 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 458 <211> 183 <212> PRT <213> Human coxsackievirus <400> 458 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 Thr Ala Pro Thr Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 Thr Asn Asp Ala Val Leu Ala Val Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 459 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A1_Tompkins_3C(T20P) <400> 459 ggc cca gta ttt gat tat gca gtt gca atg gca aag aaa aac atc ctg 48 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 aca gcc acc ccn gaa aag ggt gaa ttc aca atg tta gga gta cat gat 96 Thr Ala Thr Pro Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aga gtt gca gta ttg cct acc cat tca aac cca ggt gaa aca ata gtt 144 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 gtg tca gga aaa gaa gtg aag atc ttg gat gca aag agg ctg gtt gat 192 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 aat gat gag gtc aat ctt gaa att act att gta acc cta gac aga aac 240 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 gaa aag ttt aga gac atc aga act cac tta ccg acc caa atc cat gag 288 Glu Lys Phe Arg Asp Ile Arg Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 acc aat gat gcg gtt ttg gct gtc aac acc tcc aaa ttt cca aat atg 336 Thr Asn Asp Ala Val Leu Ala Val Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tac att cca gtt gga tca gta gtt gaa cag gga atg ttg aac ctt ggt 384 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 ggc aga ccc aca aat aga acc cta atg tat aac ttt cca act aaa gca 432 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 ggg cag tgt ggt ggt gtc tta atg gcc act ggc aaa gtg att ggc ata 480 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 cat gtt ggt ggt aat gga tct cac ggt ttc gca gct gca ctt aaa aga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 agt tac ttt act gaa gaa cag 549 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 460 <211> 183 <212> PRT <213> Human coxsackievirus <400> 460 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 Thr Ala Thr Pro Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 Thr Asn Asp Ala Val Leu Ala Val Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 461 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A1_Tompkins_3C(wt) <400> 461 ggc cca gta ttt gat tat gca gtt gca atg gca aag aaa aac atc ctg 48 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 aca gcc acc aca gaa aag ggt gaa ttc aca atg tta gga gta cat gat 96 Thr Ala Thr Thr Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aga gtt gca gta ttg cct acc cat tca aac cca ggt gaa aca ata gtt 144 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 gtg tca gga aaa gaa gtg aag atc ttg gat gca aag agg ctg gtt gat 192 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 aat gat gag gtc aat ctt gaa att act att gta acc cta gac aga aac 240 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 gaa aag ttt aga gac atc aga act cac tta ccg acc caa atc cat gag 288 Glu Lys Phe Arg Asp Ile Arg Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 acc aat gat gcg gtt ttg gct gtc aac acc tcc aaa ttt cca aat atg 336 Thr Asn Asp Ala Val Leu Ala Val Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tac att cca gtt gga tca gta gtt gaa cag gga atg ttg aac ctt ggt 384 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 ggc aga ccc aca aat aga acc cta atg tat aac ttt cca act aaa gca 432 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 ggg cag tgt ggt ggt gtc tta atg gcc act ggc aaa gtg att ggc ata 480 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 cat gtt ggt ggt aat gga tct cac ggt ttc gca gct gca ctt aaa aga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 agt tac ttt act gaa gaa cag 549 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 462 <211> 183 <212> PRT <213> Human coxsackievirus <400> 462 Gly Pro Val Phe Asp Tyr Ala Val Ala Met Ala Lys Lys Asn Ile Leu 1 5 10 15 Thr Ala Thr Thr Glu Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Arg Val Ala Val Leu Pro Thr His Ser Asn Pro Gly Glu Thr Ile Val 35 40 45 Val Ser Gly Lys Glu Val Lys Ile Leu Asp Ala Lys Arg Leu Val Asp 50 55 60 Asn Asp Glu Val Asn Leu Glu Ile Thr Ile Val Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Thr His Leu Pro Thr Gln Ile His Glu 85 90 95 Thr Asn Asp Ala Val Leu Ala Val Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Ser Val Val Glu Gln Gly Met Leu Asn Leu Gly 115 120 125 Gly Arg Pro Thr Asn Arg Thr Leu Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ala Thr Gly Lys Val Ile Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Glu Glu Gln 180 <210> 463 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A10_Kowalik_3C(L102P) <400> 463 gga cct agc ttg gac ttc gct ttg tct ctt ctg agg cgc aac atc aga 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gcg cag acc gac cag gga cac ttc acc atg cta ggc ata cgg gac 96 Gln Ala Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 cgt cta gcc atc ttg cca cgc cac tca caa cca ggg aag acc atc tgg 144 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 ata gag cac aaa ttg gtc aac gta cta gat gca gtt gag ttg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa ggt gtt aat ttg gaa ctc acg ctg gtg acc ttg gac act aat 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 gag aag ttt agg gac att acc aag ttc atc cca gag aca ata gct ggt 288 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 gct agt gat gca act ccn gtt atc aac act gag cat atg ccc tcg atg 336 Ala Ser Asp Ala Thr Pro Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 ttt gtg cca ata ggt gac gtt gtg cag tat ggg ttt ttg aat ctc agt 384 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggc aaa ccc aca cac aga act atg atg tac aat ttc ccc acg aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt ggg ggg gta gtc act tca gtt ggc aag atc att gga atc 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 cac att ggc ggg aat gga cgc cag ggc ttc tgc gct ggt tta aag agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agc tac ttt gcc agc gag caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 464 <211> 183 <212> PRT <213> Human coxsackievirus <400> 464 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Ala Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 Ala Ser Asp Ala Thr Pro Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 465 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A10_Kowalik_3C(M109P) <400> 465 gga cct agc ttg gac ttc gct ttg tct ctt ctg agg cgc aac atc aga 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gcg cag acc gac cag gga cac ttc acc atg cta ggc ata cgg gac 96 Gln Ala Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 cgt cta gcc atc ttg cca cgc cac tca caa cca ggg aag acc atc tgg 144 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 ata gag cac aaa ttg gtc aac gta cta gat gca gtt gag ttg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa ggt gtt aat ttg gaa ctc acg ctg gtg acc ttg gac act aat 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 gag aag ttt agg gac att acc aag ttc atc cca gag aca ata gct ggt 288 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 gct agt gat gca act cta gtt atc aac act gag cat ccn ccc tcg atg 336 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Pro Pro Ser Met 100 105 110 ttt gtg cca ata ggt gac gtt gtg cag tat ggg ttt ttg aat ctc agt 384 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggc aaa ccc aca cac aga act atg atg tac aat ttc ccc acg aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt ggg ggg gta gtc act tca gtt ggc aag atc att gga atc 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 cac att ggc ggg aat gga cgc cag ggc ttc tgc gct ggt tta aag agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agc tac ttt gcc agc gag caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 466 <211> 183 <212> PRT <213> Human coxsackievirus <400> 466 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Ala Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Pro Pro Ser Met 100 105 110 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 467 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A10_Kowalik_3C(N105P) <400> 467 gga cct agc ttg gac ttc gct ttg tct ctt ctg agg cgc aac atc aga 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gcg cag acc gac cag gga cac ttc acc atg cta ggc ata cgg gac 96 Gln Ala Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 cgt cta gcc atc ttg cca cgc cac tca caa cca ggg aag acc atc tgg 144 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 ata gag cac aaa ttg gtc aac gta cta gat gca gtt gag ttg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa ggt gtt aat ttg gaa ctc acg ctg gtg acc ttg gac act aat 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 gag aag ttt agg gac att acc aag ttc atc cca gag aca ata gct ggt 288 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 gct agt gat gca act cta gtt atc ccn act gag cat atg ccc tcg atg 336 Ala Ser Asp Ala Thr Leu Val Ile Pro Thr Glu His Met Pro Ser Met 100 105 110 ttt gtg cca ata ggt gac gtt gtg cag tat ggg ttt ttg aat ctc agt 384 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggc aaa ccc aca cac aga act atg atg tac aat ttc ccc acg aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt ggg ggg gta gtc act tca gtt ggc aag atc att gga atc 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 cac att ggc ggg aat gga cgc cag ggc ttc tgc gct ggt tta aag agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agc tac ttt gcc agc gag caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 468 <211> 183 <212> PRT <213> Human coxsackievirus <400> 468 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Ala Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 Ala Ser Asp Ala Thr Leu Val Ile Pro Thr Glu His Met Pro Ser Met 100 105 110 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 469 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A10_Kowalik_3C(Q19P) <400> 469 gga cct agc ttg gac ttc gct ttg tct ctt ctg agg cgc aac atc aga 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gcg ccn acc gac cag gga cac ttc acc atg cta ggc ata cgg gac 96 Gln Ala Pro Thr Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 cgt cta gcc atc ttg cca cgc cac tca caa cca ggg aag acc atc tgg 144 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 ata gag cac aaa ttg gtc aac gta cta gat gca gtt gag ttg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa ggt gtt aat ttg gaa ctc acg ctg gtg acc ttg gac act aat 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 gag aag ttt agg gac att acc aag ttc atc cca gag aca ata gct ggt 288 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 gct agt gat gca act cta gtt atc aac act gag cat atg ccc tcg atg 336 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 ttt gtg cca ata ggt gac gtt gtg cag tat ggg ttt ttg aat ctc agt 384 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggc aaa ccc aca cac aga act atg atg tac aat ttc ccc acg aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt ggg ggg gta gtc act tca gtt ggc aag atc att gga atc 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 cac att ggc ggg aat gga cgc cag ggc ttc tgc gct ggt tta aag agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agc tac ttt gcc agc gag caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 470 <211> 183 <212> PRT <213> Human coxsackievirus <400> 470 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Ala Pro Thr Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 471 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A10_Kowalik_3C(T106P) <400> 471 gga cct agc ttg gac ttc gct ttg tct ctt ctg agg cgc aac atc aga 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gcg cag acc gac cag gga cac ttc acc atg cta ggc ata cgg gac 96 Gln Ala Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 cgt cta gcc atc ttg cca cgc cac tca caa cca ggg aag acc atc tgg 144 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 ata gag cac aaa ttg gtc aac gta cta gat gca gtt gag ttg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa ggt gtt aat ttg gaa ctc acg ctg gtg acc ttg gac act aat 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 gag aag ttt agg gac att acc aag ttc atc cca gag aca ata gct ggt 288 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 gct agt gat gca act cta gtt atc aac ccn gag cat atg ccc tcg atg 336 Ala Ser Asp Ala Thr Leu Val Ile Asn Pro Glu His Met Pro Ser Met 100 105 110 ttt gtg cca ata ggt gac gtt gtg cag tat ggg ttt ttg aat ctc agt 384 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggc aaa ccc aca cac aga act atg atg tac aat ttc ccc acg aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt ggg ggg gta gtc act tca gtt ggc aag atc att gga atc 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 cac att ggc ggg aat gga cgc cag ggc ttc tgc gct ggt tta aag agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agc tac ttt gcc agc gag caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 472 <211> 183 <212> PRT <213> Human coxsackievirus <400> 472 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Ala Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 Ala Ser Asp Ala Thr Leu Val Ile Asn Pro Glu His Met Pro Ser Met 100 105 110 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 473 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A10_Kowalik_3C(T20P) <400> 473 gga cct agc ttg gac ttc gct ttg tct ctt ctg agg cgc aac atc aga 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gcg cag ccn gac cag gga cac ttc acc atg cta ggc ata cgg gac 96 Gln Ala Gln Pro Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 cgt cta gcc atc ttg cca cgc cac tca caa cca ggg aag acc atc tgg 144 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 ata gag cac aaa ttg gtc aac gta cta gat gca gtt gag ttg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa ggt gtt aat ttg gaa ctc acg ctg gtg acc ttg gac act aat 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 gag aag ttt agg gac att acc aag ttc atc cca gag aca ata gct ggt 288 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 gct agt gat gca act cta gtt atc aac act gag cat atg ccc tcg atg 336 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 ttt gtg cca ata ggt gac gtt gtg cag tat ggg ttt ttg aat ctc agt 384 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggc aaa ccc aca cac aga act atg atg tac aat ttc ccc acg aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt ggg ggg gta gtc act tca gtt ggc aag atc att gga atc 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 cac att ggc ggg aat gga cgc cag ggc ttc tgc gct ggt tta aag agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agc tac ttt gcc agc gag caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 474 <211> 183 <212> PRT <213> Human coxsackievirus <400> 474 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Ala Gln Pro Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 475 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A10_Kowalik_3C(T87P) <400> 475 gga cct agc ttg gac ttc gct ttg tct ctt ctg agg cgc aac atc aga 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gcg cag acc gac cag gga cac ttc acc atg cta ggc ata cgg gac 96 Gln Ala Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 cgt cta gcc atc ttg cca cgc cac tca caa cca ggg aag acc atc tgg 144 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 ata gag cac aaa ttg gtc aac gta cta gat gca gtt gag ttg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa ggt gtt aat ttg gaa ctc acg ctg gtg acc ttg gac act aat 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 gag aag ttt agg gac att ccn aag ttc atc cca gag aca ata gct ggt 288 Glu Lys Phe Arg Asp Ile Pro Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 gct agt gat gca act cta gtt atc aac act gag cat atg ccc tcg atg 336 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 ttt gtg cca ata ggt gac gtt gtg cag tat ggg ttt ttg aat ctc agt 384 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggc aaa ccc aca cac aga act atg atg tac aat ttc ccc acg aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt ggg ggg gta gtc act tca gtt ggc aag atc att gga atc 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 cac att ggc ggg aat gga cgc cag ggc ttc tgc gct ggt tta aag agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agc tac ttt gcc agc gag caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 476 <211> 183 <212> PRT <213> Human coxsackievirus <400> 476 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Ala Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Pro Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 477 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_A10_Kowalik_3C(wt) <400> 477 gga cct agc ttg gac ttc gct ttg tct ctt ctg agg cgc aac atc aga 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gcg cag acc gac cag gga cac ttc acc atg cta ggc ata cgg gac 96 Gln Ala Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 cgt cta gcc atc ttg cca cgc cac tca caa cca ggg aag acc atc tgg 144 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 ata gag cac aaa ttg gtc aac gta cta gat gca gtt gag ttg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa ggt gtt aat ttg gaa ctc acg ctg gtg acc ttg gac act aat 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 gag aag ttt agg gac att acc aag ttc atc cca gag aca ata gct ggt 288 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 gct agt gat gca act cta gtt atc aac act gag cat atg ccc tcg atg 336 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 ttt gtg cca ata ggt gac gtt gtg cag tat ggg ttt ttg aat ctc agt 384 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggc aaa ccc aca cac aga act atg atg tac aat ttc ccc acg aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt ggg ggg gta gtc act tca gtt ggc aag atc att gga atc 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 cac att ggc ggg aat gga cgc cag ggc ttc tgc gct ggt tta aag agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agc tac ttt gcc agc gag caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 478 <211> 183 <212> PRT <213> Human coxsackievirus <400> 478 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Ala Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Ile Arg Asp 20 25 30 Arg Leu Ala Ile Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Thr Ile Ala Gly 85 90 95 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 Phe Val Pro Ile Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Ile Ile Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 479 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B1_3C(F109P) <400> 479 gga cct gta ttt gag ttt gcc gta gcc atg atg aag agg aat tcg agc 48 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 aca gtg aaa act gaa tac ggt gag ttc act atg ctg ggt atc tat gat 96 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gct gtt ttg cca cgt cat gcc aaa cct ggg cca acc atc tta 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gaa att ggt gtg cta gac gcc aag gag tta gtg gac 192 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggt aca aac ttg gaa ctg aca cta ctt aag ctg aac cga aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gaa aag ttc aga gat att agg ggc ttc tta gcc aag gaa gag gtg gag 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gct gtg cta gca att aac acc agc aag ccn cct aac atg 336 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Pro Pro Asn Met 100 105 110 tat atc cca gtg gga caa gtt aca gag tat ggc ttc ctg aat ctg ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggt aca ccc acc aag aga atg ctt atg tac aac ttc ccc acg agg gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggc gga gtg ctc atg tcc act ggc aaa gtt ttg ggg atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggc ggg aat ggt cat caa ggc ttc tca gcg gca ctt ctt aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gaa caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 480 <211> 183 <212> PRT <213> Human coxsackievirus <400> 480 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Pro Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 481 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B1_3C(K19P) <400> 481 gga cct gta ttt gag ttt gcc gta gcc atg atg aag agg aat tcg agc 48 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 aca gtg ccn act gaa tac ggt gag ttc act atg ctg ggt atc tat gat 96 Thr Val Pro Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gct gtt ttg cca cgt cat gcc aaa cct ggg cca acc atc tta 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gaa att ggt gtg cta gac gcc aag gag tta gtg gac 192 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggt aca aac ttg gaa ctg aca cta ctt aag ctg aac cga aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gaa aag ttc aga gat att agg ggc ttc tta gcc aag gaa gag gtg gag 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gct gtg cta gca att aac acc agc aag ttc cct aac atg 336 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tat atc cca gtg gga caa gtt aca gag tat ggc ttc ctg aat ctg ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggt aca ccc acc aag aga atg ctt atg tac aac ttc ccc acg agg gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggc gga gtg ctc atg tcc act ggc aaa gtt ttg ggg atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggc ggg aat ggt cat caa ggc ttc tca gcg gca ctt ctt aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gaa caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 482 <211> 183 <212> PRT <213> Human coxsackievirus <400> 482 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Pro Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 483 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B1_3C(L102P) <400> 483 gga cct gta ttt gag ttt gcc gta gcc atg atg aag agg aat tcg agc 48 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 aca gtg aaa act gaa tac ggt gag ttc act atg ctg ggt atc tat gat 96 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gct gtt ttg cca cgt cat gcc aaa cct ggg cca acc atc tta 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gaa att ggt gtg cta gac gcc aag gag tta gtg gac 192 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggt aca aac ttg gaa ctg aca cta ctt aag ctg aac cga aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gaa aag ttc aga gat att agg ggc ttc tta gcc aag gaa gag gtg gag 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gct gtg ccn gca att aac acc agc aag ttc cct aac atg 336 Val Asn Glu Ala Val Pro Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tat atc cca gtg gga caa gtt aca gag tat ggc ttc ctg aat ctg ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggt aca ccc acc aag aga atg ctt atg tac aac ttc ccc acg agg gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggc gga gtg ctc atg tcc act ggc aaa gtt ttg ggg atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggc ggg aat ggt cat caa ggc ttc tca gcg gca ctt ctt aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gaa caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 484 <211> 183 <212> PRT <213> Human coxsackievirus <400> 484 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Pro Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 485 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B1_3C(N105P) <400> 485 gga cct gta ttt gag ttt gcc gta gcc atg atg aag agg aat tcg agc 48 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 aca gtg aaa act gaa tac ggt gag ttc act atg ctg ggt atc tat gat 96 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gct gtt ttg cca cgt cat gcc aaa cct ggg cca acc atc tta 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gaa att ggt gtg cta gac gcc aag gag tta gtg gac 192 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggt aca aac ttg gaa ctg aca cta ctt aag ctg aac cga aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gaa aag ttc aga gat att agg ggc ttc tta gcc aag gaa gag gtg gag 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gct gtg cta gca att ccn acc agc aag ttc cct aac atg 336 Val Asn Glu Ala Val Leu Ala Ile Pro Thr Ser Lys Phe Pro Asn Met 100 105 110 tat atc cca gtg gga caa gtt aca gag tat ggc ttc ctg aat ctg ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggt aca ccc acc aag aga atg ctt atg tac aac ttc ccc acg agg gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggc gga gtg ctc atg tcc act ggc aaa gtt ttg ggg atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggc ggg aat ggt cat caa ggc ttc tca gcg gca ctt ctt aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gaa caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 486 <211> 183 <212> PRT <213> Human coxsackievirus <400> 486 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Pro Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 487 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B1_3C(R87P) <400> 487 gga cct gta ttt gag ttt gcc gta gcc atg atg aag agg aat tcg agc 48 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 aca gtg aaa act gaa tac ggt gag ttc act atg ctg ggt atc tat gat 96 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gct gtt ttg cca cgt cat gcc aaa cct ggg cca acc atc tta 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gaa att ggt gtg cta gac gcc aag gag tta gtg gac 192 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggt aca aac ttg gaa ctg aca cta ctt aag ctg aac cga aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gaa aag ttc aga gat att ccn ggc ttc tta gcc aag gaa gag gtg gag 288 Glu Lys Phe Arg Asp Ile Pro Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gct gtg cta gca att aac acc agc aag ttc cct aac atg 336 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tat atc cca gtg gga caa gtt aca gag tat ggc ttc ctg aat ctg ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggt aca ccc acc aag aga atg ctt atg tac aac ttc ccc acg agg gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggc gga gtg ctc atg tcc act ggc aaa gtt ttg ggg atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggc ggg aat ggt cat caa ggc ttc tca gcg gca ctt ctt aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gaa caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 488 <211> 183 <212> PRT <213> Human coxsackievirus <400> 488 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Pro Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 489 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B1_3C(T106P) <400> 489 gga cct gta ttt gag ttt gcc gta gcc atg atg aag agg aat tcg agc 48 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 aca gtg aaa act gaa tac ggt gag ttc act atg ctg ggt atc tat gat 96 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gct gtt ttg cca cgt cat gcc aaa cct ggg cca acc atc tta 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gaa att ggt gtg cta gac gcc aag gag tta gtg gac 192 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggt aca aac ttg gaa ctg aca cta ctt aag ctg aac cga aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gaa aag ttc aga gat att agg ggc ttc tta gcc aag gaa gag gtg gag 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gct gtg cta gca att aac ccn agc aag ttc cct aac atg 336 Val Asn Glu Ala Val Leu Ala Ile Asn Pro Ser Lys Phe Pro Asn Met 100 105 110 tat atc cca gtg gga caa gtt aca gag tat ggc ttc ctg aat ctg ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggt aca ccc acc aag aga atg ctt atg tac aac ttc ccc acg agg gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggc gga gtg ctc atg tcc act ggc aaa gtt ttg ggg atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggc ggg aat ggt cat caa ggc ttc tca gcg gca ctt ctt aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gaa caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 490 <211> 183 <212> PRT <213> Human coxsackievirus <400> 490 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Asn Pro Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 491 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B1_3C(T20P) <400> 491 gga cct gta ttt gag ttt gcc gta gcc atg atg aag agg aat tcg agc 48 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 aca gtg aaa ccn gaa tac ggt gag ttc act atg ctg ggt atc tat gat 96 Thr Val Lys Pro Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gct gtt ttg cca cgt cat gcc aaa cct ggg cca acc atc tta 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gaa att ggt gtg cta gac gcc aag gag tta gtg gac 192 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggt aca aac ttg gaa ctg aca cta ctt aag ctg aac cga aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gaa aag ttc aga gat att agg ggc ttc tta gcc aag gaa gag gtg gag 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gct gtg cta gca att aac acc agc aag ttc cct aac atg 336 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tat atc cca gtg gga caa gtt aca gag tat ggc ttc ctg aat ctg ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggt aca ccc acc aag aga atg ctt atg tac aac ttc ccc acg agg gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggc gga gtg ctc atg tcc act ggc aaa gtt ttg ggg atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggc ggg aat ggt cat caa ggc ttc tca gcg gca ctt ctt aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gaa caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 492 <211> 183 <212> PRT <213> Human coxsackievirus <400> 492 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Lys Pro Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 493 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B1_3C(wt) <400> 493 gga cct gta ttt gag ttt gcc gta gcc atg atg aag agg aat tcg agc 48 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 aca gtg aaa act gaa tac ggt gag ttc act atg ctg ggt atc tat gat 96 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gct gtt ttg cca cgt cat gcc aaa cct ggg cca acc atc tta 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gaa att ggt gtg cta gac gcc aag gag tta gtg gac 192 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggt aca aac ttg gaa ctg aca cta ctt aag ctg aac cga aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gaa aag ttc aga gat att agg ggc ttc tta gcc aag gaa gag gtg gag 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gct gtg cta gca att aac acc agc aag ttc cct aac atg 336 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tat atc cca gtg gga caa gtt aca gag tat ggc ttc ctg aat ctg ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggt aca ccc acc aag aga atg ctt atg tac aac ttc ccc acg agg gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggc gga gtg ctc atg tcc act ggc aaa gtt ttg ggg atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggc ggg aat ggt cat caa ggc ttc tca gcg gca ctt ctt aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gaa caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 494 <211> 183 <212> PRT <213> Human coxsackievirus <400> 494 Gly Pro Val Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Ile Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 495 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B3_Macocy_3C(F109P) <400> 495 ggc cct gcc ttt gag ttc gcc gtc gca atg atg aaa agg aac tca agc 48 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 acg gtg aag act gaa tat ggc gag ttt acc atg ctg ggc atc tat gac 96 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gcc gtt ttg cca cgc cac gcc aaa cct ggg cca acc atc ttg 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gag gtt ggt gtg cta gat gcc aag gag cta gta gac 192 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggc acc aac tta gaa ctg aca cta ctc aaa ttg aac cgg aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gag aag ttc aga gac atc aga ggc ttc tta gcc aag gag gaa gtg gag 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gca gtg cta gca att aac acc agc aag ccn ccc aac atg 336 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Pro Pro Asn Met 100 105 110 tac att cca gta gga cag gtc aca gaa tac ggc ttc cta aac cta ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggc aca ccc acc aag aga atg ctt atg tac aac ttc ccc aca aga gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggc cag tgt ggt gga gtg ctc atg tcc acc ggc aag gta ctg ggt atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggt gga aat ggc cat cag ggc ttc tca gca gca ctc ctc aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gag caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 496 <211> 183 <212> PRT <213> Human coxsackievirus <400> 496 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Pro Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 497 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B3_Macocy_3C(K19P) <400> 497 ggc cct gcc ttt gag ttc gcc gtc gca atg atg aaa agg aac tca agc 48 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 acg gtg ccn act gaa tat ggc gag ttt acc atg ctg ggc atc tat gac 96 Thr Val Pro Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gcc gtt ttg cca cgc cac gcc aaa cct ggg cca acc atc ttg 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gag gtt ggt gtg cta gat gcc aag gag cta gta gac 192 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggc acc aac tta gaa ctg aca cta ctc aaa ttg aac cgg aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gag aag ttc aga gac atc aga ggc ttc tta gcc aag gag gaa gtg gag 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gca gtg cta gca att aac acc agc aag ttt ccc aac atg 336 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tac att cca gta gga cag gtc aca gaa tac ggc ttc cta aac cta ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggc aca ccc acc aag aga atg ctt atg tac aac ttc ccc aca aga gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggc cag tgt ggt gga gtg ctc atg tcc acc ggc aag gta ctg ggt atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggt gga aat ggc cat cag ggc ttc tca gca gca ctc ctc aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gag caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 498 <211> 183 <212> PRT <213> Human coxsackievirus <400> 498 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Pro Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 499 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B3_Macocy_3C(L102P) <400> 499 ggc cct gcc ttt gag ttc gcc gtc gca atg atg aaa agg aac tca agc 48 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 acg gtg aag act gaa tat ggc gag ttt acc atg ctg ggc atc tat gac 96 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gcc gtt ttg cca cgc cac gcc aaa cct ggg cca acc atc ttg 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gag gtt ggt gtg cta gat gcc aag gag cta gta gac 192 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggc acc aac tta gaa ctg aca cta ctc aaa ttg aac cgg aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gag aag ttc aga gac atc aga ggc ttc tta gcc aag gag gaa gtg gag 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gca gtg ccn gca att aac acc agc aag ttt ccc aac atg 336 Val Asn Glu Ala Val Pro Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tac att cca gta gga cag gtc aca gaa tac ggc ttc cta aac cta ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggc aca ccc acc aag aga atg ctt atg tac aac ttc ccc aca aga gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggc cag tgt ggt gga gtg ctc atg tcc acc ggc aag gta ctg ggt atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggt gga aat ggc cat cag ggc ttc tca gca gca ctc ctc aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gag caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 500 <211> 183 <212> PRT <213> Human coxsackievirus <400> 500 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Pro Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 501 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B3_Macocy_3C(N105P) <400> 501 ggc cct gcc ttt gag ttc gcc gtc gca atg atg aaa agg aac tca agc 48 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 acg gtg aag act gaa tat ggc gag ttt acc atg ctg ggc atc tat gac 96 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gcc gtt ttg cca cgc cac gcc aaa cct ggg cca acc atc ttg 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gag gtt ggt gtg cta gat gcc aag gag cta gta gac 192 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggc acc aac tta gaa ctg aca cta ctc aaa ttg aac cgg aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gag aag ttc aga gac atc aga ggc ttc tta gcc aag gag gaa gtg gag 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gca gtg cta gca att ccn acc agc aag ttt ccc aac atg 336 Val Asn Glu Ala Val Leu Ala Ile Pro Thr Ser Lys Phe Pro Asn Met 100 105 110 tac att cca gta gga cag gtc aca gaa tac ggc ttc cta aac cta ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggc aca ccc acc aag aga atg ctt atg tac aac ttc ccc aca aga gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggc cag tgt ggt gga gtg ctc atg tcc acc ggc aag gta ctg ggt atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggt gga aat ggc cat cag ggc ttc tca gca gca ctc ctc aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gag caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 502 <211> 183 <212> PRT <213> Human coxsackievirus <400> 502 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Pro Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 503 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B3_Macocy_3C(R87P) <400> 503 ggc cct gcc ttt gag ttc gcc gtc gca atg atg aaa agg aac tca agc 48 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 acg gtg aag act gaa tat ggc gag ttt acc atg ctg ggc atc tat gac 96 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gcc gtt ttg cca cgc cac gcc aaa cct ggg cca acc atc ttg 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gag gtt ggt gtg cta gat gcc aag gag cta gta gac 192 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggc acc aac tta gaa ctg aca cta ctc aaa ttg aac cgg aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gag aag ttc aga gac atc ccn ggc ttc tta gcc aag gag gaa gtg gag 288 Glu Lys Phe Arg Asp Ile Pro Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gca gtg cta gca att aac acc agc aag ttt ccc aac atg 336 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tac att cca gta gga cag gtc aca gaa tac ggc ttc cta aac cta ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggc aca ccc acc aag aga atg ctt atg tac aac ttc ccc aca aga gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggc cag tgt ggt gga gtg ctc atg tcc acc ggc aag gta ctg ggt atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggt gga aat ggc cat cag ggc ttc tca gca gca ctc ctc aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gag caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 504 <211> 183 <212> PRT <213> Human coxsackievirus <400> 504 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Pro Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 505 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B3_Macocy_3C(T106P) <400> 505 ggc cct gcc ttt gag ttc gcc gtc gca atg atg aaa agg aac tca agc 48 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 acg gtg aag act gaa tat ggc gag ttt acc atg ctg ggc atc tat gac 96 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gcc gtt ttg cca cgc cac gcc aaa cct ggg cca acc atc ttg 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gag gtt ggt gtg cta gat gcc aag gag cta gta gac 192 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggc acc aac tta gaa ctg aca cta ctc aaa ttg aac cgg aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gag aag ttc aga gac atc aga ggc ttc tta gcc aag gag gaa gtg gag 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gca gtg cta gca att aac ccn agc aag ttt ccc aac atg 336 Val Asn Glu Ala Val Leu Ala Ile Asn Pro Ser Lys Phe Pro Asn Met 100 105 110 tac att cca gta gga cag gtc aca gaa tac ggc ttc cta aac cta ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggc aca ccc acc aag aga atg ctt atg tac aac ttc ccc aca aga gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggc cag tgt ggt gga gtg ctc atg tcc acc ggc aag gta ctg ggt atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggt gga aat ggc cat cag ggc ttc tca gca gca ctc ctc aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gag caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 506 <211> 183 <212> PRT <213> Human coxsackievirus <400> 506 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Asn Pro Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 507 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B3_Macocy_3C(T20P) <400> 507 ggc cct gcc ttt gag ttc gcc gtc gca atg atg aaa agg aac tca agc 48 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 acg gtg aag ccn gaa tat ggc gag ttt acc atg ctg ggc atc tat gac 96 Thr Val Lys Pro Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gcc gtt ttg cca cgc cac gcc aaa cct ggg cca acc atc ttg 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gag gtt ggt gtg cta gat gcc aag gag cta gta gac 192 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggc acc aac tta gaa ctg aca cta ctc aaa ttg aac cgg aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gag aag ttc aga gac atc aga ggc ttc tta gcc aag gag gaa gtg gag 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gca gtg cta gca att aac acc agc aag ttt ccc aac atg 336 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tac att cca gta gga cag gtc aca gaa tac ggc ttc cta aac cta ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggc aca ccc acc aag aga atg ctt atg tac aac ttc ccc aca aga gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggc cag tgt ggt gga gtg ctc atg tcc acc ggc aag gta ctg ggt atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggt gga aat ggc cat cag ggc ttc tca gca gca ctc ctc aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gag caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 508 <211> 183 <212> PRT <213> Human coxsackievirus <400> 508 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Lys Pro Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 509 <211> 549 <212> DNA <213> Human coxsackievirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> Human_Coxsackievirus_B3_Macocy_3C(wt) <400> 509 ggc cct gcc ttt gag ttc gcc gtc gca atg atg aaa agg aac tca agc 48 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 acg gtg aag act gaa tat ggc gag ttt acc atg ctg ggc atc tat gac 96 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gcc gtt ttg cca cgc cac gcc aaa cct ggg cca acc atc ttg 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aat gat caa gag gtt ggt gtg cta gat gcc aag gag cta gta gac 192 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aag gac ggc acc aac tta gaa ctg aca cta ctc aaa ttg aac cgg aat 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gag aag ttc aga gac atc aga ggc ttc tta gcc aag gag gaa gtg gag 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 gtt aat gag gca gtg cta gca att aac acc agc aag ttt ccc aac atg 336 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tac att cca gta gga cag gtc aca gaa tac ggc ttc cta aac cta ggt 384 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 ggc aca ccc acc aag aga atg ctt atg tac aac ttc ccc aca aga gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggc cag tgt ggt gga gtg ctc atg tcc acc ggc aag gta ctg ggt atc 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gtt ggt gga aat ggc cat cag ggc ttc tca gca gca ctc ctc aaa 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 cac tac ttc aat gat gag caa 549 His Tyr Phe Asn Asp Glu Gln 180 <210> 510 <211> 183 <212> PRT <213> Human coxsackievirus <400> 510 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ser Ser 1 5 10 15 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Glu Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Lys Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Gln Val Thr Glu Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Lys 165 170 175 His Tyr Phe Asn Asp Glu Gln 180 <210> 511 <211> 549 <212> DNA <213> Human enterovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Enterovirus_A71_Strain_C4_KP861243_3C(I104P) <400> 511 ggc ccg agc ctt gac ttt gct ctc tcc cta ctg aga agg aac atc agg 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gtc caa aca gac caa ggg cat ttc acc atg ttg ggt gtt agg gat 96 Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 cgc tta gca gtc ctc cca cgc cac tca caa cct ggc aaa acc att tgg 144 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 att gag cac aaa ctc gtg aac gtc ctt gat gca gtt gaa ctg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa gga gtc aac ctg gaa tta acc ctc atc act ctt gac acc aac 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 gaa aag ttt agg gat atc acc aaa ttc atc cca gaa aat atc agc act 288 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 gct agc gat gcc acc cta gtg ccn aac acg gag cac atg cca tca atg 336 Ala Ser Asp Ala Thr Leu Val Pro Asn Thr Glu His Met Pro Ser Met 100 105 110 ttt gtc ccg gtg ggt gac gtt gtg cag tat ggc ttt ttg aat ctc agt 384 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggt aag cct acc cat cgc acc atg atg tac aat ttt cct acc aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt gga gga gtg gtg acg tct gtt ggg aag gtt gtc ggt att 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 cac att ggt ggc aat ggc aga caa ggt ttt tgc gca ggc ctc aaa agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agt tac ttt gct agt gaa caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 512 <211> 183 <212> PRT <213> Human enterovirus <400> 512 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 Ala Ser Asp Ala Thr Leu Val Pro Asn Thr Glu His Met Pro Ser Met 100 105 110 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 513 <211> 549 <212> DNA <213> Human enterovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Enterovirus_A71_Strain_C4_KP861243_3C(M109P) <400> 513 ggc ccg agc ctt gac ttt gct ctc tcc cta ctg aga agg aac atc agg 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gtc caa aca gac caa ggg cat ttc acc atg ttg ggt gtt agg gat 96 Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 cgc tta gca gtc ctc cca cgc cac tca caa cct ggc aaa acc att tgg 144 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 att gag cac aaa ctc gtg aac gtc ctt gat gca gtt gaa ctg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa gga gtc aac ctg gaa tta acc ctc atc act ctt gac acc aac 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 gaa aag ttt agg gat atc acc aaa ttc atc cca gaa aat atc agc act 288 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 gct agc gat gcc acc cta gtg atc aac acg gag cac ccn cca tca atg 336 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Pro Pro Ser Met 100 105 110 ttt gtc ccg gtg ggt gac gtt gtg cag tat ggc ttt ttg aat ctc agt 384 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggt aag cct acc cat cgc acc atg atg tac aat ttt cct acc aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt gga gga gtg gtg acg tct gtt ggg aag gtt gtc ggt att 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 cac att ggt ggc aat ggc aga caa ggt ttt tgc gca ggc ctc aaa agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agt tac ttt gct agt gaa caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 514 <211> 183 <212> PRT <213> Human enterovirus <400> 514 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Pro Pro Ser Met 100 105 110 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 515 <211> 549 <212> DNA <213> Human enterovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Enterovirus_A71_Strain_C4_KP861243_3C(N105P) <400> 515 ggc ccg agc ctt gac ttt gct ctc tcc cta ctg aga agg aac atc agg 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gtc caa aca gac caa ggg cat ttc acc atg ttg ggt gtt agg gat 96 Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 cgc tta gca gtc ctc cca cgc cac tca caa cct ggc aaa acc att tgg 144 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 att gag cac aaa ctc gtg aac gtc ctt gat gca gtt gaa ctg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa gga gtc aac ctg gaa tta acc ctc atc act ctt gac acc aac 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 gaa aag ttt agg gat atc acc aaa ttc atc cca gaa aat atc agc act 288 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 gct agc gat gcc acc cta gtg atc ccn acg gag cac atg cca tca atg 336 Ala Ser Asp Ala Thr Leu Val Ile Pro Thr Glu His Met Pro Ser Met 100 105 110 ttt gtc ccg gtg ggt gac gtt gtg cag tat ggc ttt ttg aat ctc agt 384 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggt aag cct acc cat cgc acc atg atg tac aat ttt cct acc aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt gga gga gtg gtg acg tct gtt ggg aag gtt gtc ggt att 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 cac att ggt ggc aat ggc aga caa ggt ttt tgc gca ggc ctc aaa agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agt tac ttt gct agt gaa caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 516 <211> 183 <212> PRT <213> Human enterovirus <400> 516 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 Ala Ser Asp Ala Thr Leu Val Ile Pro Thr Glu His Met Pro Ser Met 100 105 110 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 517 <211> 549 <212> DNA <213> Human enterovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Enterovirus_A71_Strain_C4_KP861243_3C(Q19P) <400> 517 ggc ccg agc ctt gac ttt gct ctc tcc cta ctg aga agg aac atc agg 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gtc ccn aca gac caa ggg cat ttc acc atg ttg ggt gtt agg gat 96 Gln Val Pro Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 cgc tta gca gtc ctc cca cgc cac tca caa cct ggc aaa acc att tgg 144 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 att gag cac aaa ctc gtg aac gtc ctt gat gca gtt gaa ctg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa gga gtc aac ctg gaa tta acc ctc atc act ctt gac acc aac 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 gaa aag ttt agg gat atc acc aaa ttc atc cca gaa aat atc agc act 288 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 gct agc gat gcc acc cta gtg atc aac acg gag cac atg cca tca atg 336 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 ttt gtc ccg gtg ggt gac gtt gtg cag tat ggc ttt ttg aat ctc agt 384 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggt aag cct acc cat cgc acc atg atg tac aat ttt cct acc aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt gga gga gtg gtg acg tct gtt ggg aag gtt gtc ggt att 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 cac att ggt ggc aat ggc aga caa ggt ttt tgc gca ggc ctc aaa agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agt tac ttt gct agt gaa caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 518 <211> 183 <212> PRT <213> Human enterovirus <400> 518 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Val Pro Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 519 <211> 549 <212> DNA <213> Human enterovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Enterovirus_A71_Strain_C4_KP861243_3C(T106P) <400> 519 ggc ccg agc ctt gac ttt gct ctc tcc cta ctg aga agg aac atc agg 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gtc caa aca gac caa ggg cat ttc acc atg ttg ggt gtt agg gat 96 Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 cgc tta gca gtc ctc cca cgc cac tca caa cct ggc aaa acc att tgg 144 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 att gag cac aaa ctc gtg aac gtc ctt gat gca gtt gaa ctg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa gga gtc aac ctg gaa tta acc ctc atc act ctt gac acc aac 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 gaa aag ttt agg gat atc acc aaa ttc atc cca gaa aat atc agc act 288 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 gct agc gat gcc acc cta gtg atc aac ccn gag cac atg cca tca atg 336 Ala Ser Asp Ala Thr Leu Val Ile Asn Pro Glu His Met Pro Ser Met 100 105 110 ttt gtc ccg gtg ggt gac gtt gtg cag tat ggc ttt ttg aat ctc agt 384 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggt aag cct acc cat cgc acc atg atg tac aat ttt cct acc aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt gga gga gtg gtg acg tct gtt ggg aag gtt gtc ggt att 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 cac att ggt ggc aat ggc aga caa ggt ttt tgc gca ggc ctc aaa agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agt tac ttt gct agt gaa caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 520 <211> 183 <212> PRT <213> Human enterovirus <400> 520 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 Ala Ser Asp Ala Thr Leu Val Ile Asn Pro Glu His Met Pro Ser Met 100 105 110 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 521 <211> 549 <212> DNA <213> Human enterovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Enterovirus_A71_Strain_C4_KP861243_3C(T20P) <400> 521 ggc ccg agc ctt gac ttt gct ctc tcc cta ctg aga agg aac atc agg 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gtc caa ccn gac caa ggg cat ttc acc atg ttg ggt gtt agg gat 96 Gln Val Gln Pro Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 cgc tta gca gtc ctc cca cgc cac tca caa cct ggc aaa acc att tgg 144 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 att gag cac aaa ctc gtg aac gtc ctt gat gca gtt gaa ctg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa gga gtc aac ctg gaa tta acc ctc atc act ctt gac acc aac 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 gaa aag ttt agg gat atc acc aaa ttc atc cca gaa aat atc agc act 288 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 gct agc gat gcc acc cta gtg atc aac acg gag cac atg cca tca atg 336 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 ttt gtc ccg gtg ggt gac gtt gtg cag tat ggc ttt ttg aat ctc agt 384 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggt aag cct acc cat cgc acc atg atg tac aat ttt cct acc aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt gga gga gtg gtg acg tct gtt ggg aag gtt gtc ggt att 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 cac att ggt ggc aat ggc aga caa ggt ttt tgc gca ggc ctc aaa agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agt tac ttt gct agt gaa caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 522 <211> 183 <212> PRT <213> Human enterovirus <400> 522 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Val Gln Pro Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 523 <211> 549 <212> DNA <213> Human enterovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Enterovirus_A71_Strain_C4_KP861243_3C(T87P) <400> 523 ggc ccg agc ctt gac ttt gct ctc tcc cta ctg aga agg aac atc agg 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gtc caa aca gac caa ggg cat ttc acc atg ttg ggt gtt agg gat 96 Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 cgc tta gca gtc ctc cca cgc cac tca caa cct ggc aaa acc att tgg 144 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 att gag cac aaa ctc gtg aac gtc ctt gat gca gtt gaa ctg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa gga gtc aac ctg gaa tta acc ctc atc act ctt gac acc aac 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 gaa aag ttt agg gat atc ccn aaa ttc atc cca gaa aat atc agc act 288 Glu Lys Phe Arg Asp Ile Pro Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 gct agc gat gcc acc cta gtg atc aac acg gag cac atg cca tca atg 336 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 ttt gtc ccg gtg ggt gac gtt gtg cag tat ggc ttt ttg aat ctc agt 384 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggt aag cct acc cat cgc acc atg atg tac aat ttt cct acc aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt gga gga gtg gtg acg tct gtt ggg aag gtt gtc ggt att 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 cac att ggt ggc aat ggc aga caa ggt ttt tgc gca ggc ctc aaa agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agt tac ttt gct agt gaa caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 524 <211> 183 <212> PRT <213> Human enterovirus <400> 524 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Pro Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 525 <211> 549 <212> DNA <213> Human enterovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> Human_Enterovirus_A71_Strain_C4_KP861243_3C(wt) <400> 525 ggc ccg agc ctt gac ttt gct ctc tcc cta ctg aga agg aac atc agg 48 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 caa gtc caa aca gac caa ggg cat ttc acc atg ttg ggt gtt agg gat 96 Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 cgc tta gca gtc ctc cca cgc cac tca caa cct ggc aaa acc att tgg 144 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 att gag cac aaa ctc gtg aac gtc ctt gat gca gtt gaa ctg gtg gat 192 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 gag caa gga gtc aac ctg gaa tta acc ctc atc act ctt gac acc aac 240 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 gaa aag ttt agg gat atc acc aaa ttc atc cca gaa aat atc agc act 288 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 gct agc gat gcc acc cta gtg atc aac acg gag cac atg cca tca atg 336 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 ttt gtc ccg gtg ggt gac gtt gtg cag tat ggc ttt ttg aat ctc agt 384 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 ggt aag cct acc cat cgc acc atg atg tac aat ttt cct acc aaa gca 432 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 gga cag tgt gga gga gtg gtg acg tct gtt ggg aag gtt gtc ggt att 480 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 cac att ggt ggc aat ggc aga caa ggt ttt tgc gca ggc ctc aaa agg 528 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 agt tac ttt gct agt gaa caa 549 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 526 <211> 183 <212> PRT <213> Human enterovirus <400> 526 Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 1 5 10 15 Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 20 25 30 Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 35 40 45 Ile Glu His Lys Leu Val Asn Val Leu Asp Ala Val Glu Leu Val Asp 50 55 60 Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Thr Lys Phe Ile Pro Glu Asn Ile Ser Thr 85 90 95 Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 100 105 110 Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 115 120 125 Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 130 135 140 Gly Gln Cys Gly Gly Val Val Thr Ser Val Gly Lys Val Val Gly Ile 145 150 155 160 His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Gly Leu Lys Arg 165 170 175 Ser Tyr Phe Ala Ser Glu Gln 180 <210> 527 <211> 657 <212> DNA <213> Human hepatitus <220> <221> CDS <222> (1)..(657) <220> <221> misc_feature <222> (1)..(657) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(657) <223> Human_Hepatitis_A_Virus_NC001489_3C(E20P) <400> 527 tca act ttg gaa ata gca gga ctg gtt agg aag aac ttg gtt cag ttt 48 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 gga gtt gga ccn aag aat gga tgt gtg aga tgg gtt atg aat gcc ttg 96 Gly Val Gly Pro Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 gga gtg aaa gat gat tgg ctg ctt gtg cct tcc cat gct tat aaa ttt 144 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 gag aaa gat tat gaa atg atg gag ttt tat ttt aat aga ggt gga act 192 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 tac tat tca att tca gct ggt aat gtt gtt att caa tct ttg gat gtg 240 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 gga ttc cag gat gtt gtt ctg atg aag gtt cct aca att cct aag ttt 288 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 aga gat att act cag cat ttt att aag aaa ggg gat gtg cct aga gct 336 Arg Asp Ile Thr Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 ttg aat cgc ctg gca aca tta gtg aca act gta aat gga acc cct atg 384 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Met 115 120 125 tta att tct gag ggc cca cta aag atg gaa gag aaa gct act tat gtt 432 Leu Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 cat aag aaa aat gat ggt aca aca gtt gat tta act gtg gat cag gca 480 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 tgg aga gga aaa ggc gaa ggt ctt cct gga atg tgt ggt ggg gcc ttg 528 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 gtt tca tcg aat caa tct ata cag aat gca atc ttg ggc atc cat gtt 576 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 gct gga gga aat tca att ctt gtt gca aaa ttg gtt act caa gaa atg 624 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 ttc caa aat att gat aag aaa att gaa agt cag 657 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 528 <211> 219 <212> PRT <213> Human hepatitus <400> 528 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 Gly Val Gly Pro Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 Arg Asp Ile Thr Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Met 115 120 125 Leu Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 529 <211> 657 <212> DNA <213> Human hepatitus <220> <221> CDS <222> (1)..(657) <220> <221> misc_feature <222> (1)..(657) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(657) <223> Human_Hepatitis_A_Virus_NC001489_3C(G19P) <400> 529 tca act ttg gaa ata gca gga ctg gtt agg aag aac ttg gtt cag ttt 48 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 gga gtt ccn gag aag aat gga tgt gtg aga tgg gtt atg aat gcc ttg 96 Gly Val Pro Glu Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 gga gtg aaa gat gat tgg ctg ctt gtg cct tcc cat gct tat aaa ttt 144 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 gag aaa gat tat gaa atg atg gag ttt tat ttt aat aga ggt gga act 192 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 tac tat tca att tca gct ggt aat gtt gtt att caa tct ttg gat gtg 240 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 gga ttc cag gat gtt gtt ctg atg aag gtt cct aca att cct aag ttt 288 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 aga gat att act cag cat ttt att aag aaa ggg gat gtg cct aga gct 336 Arg Asp Ile Thr Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 ttg aat cgc ctg gca aca tta gtg aca act gta aat gga acc cct atg 384 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Met 115 120 125 tta att tct gag ggc cca cta aag atg gaa gag aaa gct act tat gtt 432 Leu Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 cat aag aaa aat gat ggt aca aca gtt gat tta act gtg gat cag gca 480 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 tgg aga gga aaa ggc gaa ggt ctt cct gga atg tgt ggt ggg gcc ttg 528 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 gtt tca tcg aat caa tct ata cag aat gca atc ttg ggc atc cat gtt 576 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 gct gga gga aat tca att ctt gtt gca aaa ttg gtt act caa gaa atg 624 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 ttc caa aat att gat aag aaa att gaa agt cag 657 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 530 <211> 219 <212> PRT <213> Human hepatitus <400> 530 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 Gly Val Pro Glu Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 Arg Asp Ile Thr Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Met 115 120 125 Leu Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 531 <211> 657 <212> DNA <213> Human hepatitus <220> <221> CDS <222> (1)..(657) <220> <221> misc_feature <222> (1)..(657) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(657) <223> Human_Hepatitis_A_Virus_NC001489_3C(I130P) <400> 531 tca act ttg gaa ata gca gga ctg gtt agg aag aac ttg gtt cag ttt 48 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 gga gtt gga gag aag aat gga tgt gtg aga tgg gtt atg aat gcc ttg 96 Gly Val Gly Glu Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 gga gtg aaa gat gat tgg ctg ctt gtg cct tcc cat gct tat aaa ttt 144 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 gag aaa gat tat gaa atg atg gag ttt tat ttt aat aga ggt gga act 192 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 tac tat tca att tca gct ggt aat gtt gtt att caa tct ttg gat gtg 240 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 gga ttc cag gat gtt gtt ctg atg aag gtt cct aca att cct aag ttt 288 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 aga gat att act cag cat ttt att aag aaa ggg gat gtg cct aga gct 336 Arg Asp Ile Thr Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 ttg aat cgc ctg gca aca tta gtg aca act gta aat gga acc cct atg 384 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Met 115 120 125 tta ccn tct gag ggc cca cta aag atg gaa gag aaa gct act tat gtt 432 Leu Pro Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 cat aag aaa aat gat ggt aca aca gtt gat tta act gtg gat cag gca 480 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 tgg aga gga aaa ggc gaa ggt ctt cct gga atg tgt ggt ggg gcc ttg 528 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 gtt tca tcg aat caa tct ata cag aat gca atc ttg ggc atc cat gtt 576 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 gct gga gga aat tca att ctt gtt gca aaa ttg gtt act caa gaa atg 624 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 ttc caa aat att gat aag aaa att gaa agt cag 657 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 532 <211> 219 <212> PRT <213> Human hepatitus <400> 532 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 Gly Val Gly Glu Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 Arg Asp Ile Thr Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Met 115 120 125 Leu Pro Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 533 <211> 657 <212> DNA <213> Human hepatitus <220> <221> CDS <222> (1)..(657) <220> <221> misc_feature <222> (1)..(657) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(657) <223> Human_Hepatitis_A_Virus_NC001489_3C(M128P) <400> 533 tca act ttg gaa ata gca gga ctg gtt agg aag aac ttg gtt cag ttt 48 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 gga gtt gga gag aag aat gga tgt gtg aga tgg gtt atg aat gcc ttg 96 Gly Val Gly Glu Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 gga gtg aaa gat gat tgg ctg ctt gtg cct tcc cat gct tat aaa ttt 144 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 gag aaa gat tat gaa atg atg gag ttt tat ttt aat aga ggt gga act 192 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 tac tat tca att tca gct ggt aat gtt gtt att caa tct ttg gat gtg 240 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 gga ttc cag gat gtt gtt ctg atg aag gtt cct aca att cct aag ttt 288 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 aga gat att act cag cat ttt att aag aaa ggg gat gtg cct aga gct 336 Arg Asp Ile Thr Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 ttg aat cgc ctg gca aca tta gtg aca act gta aat gga acc cct ccn 384 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Pro 115 120 125 tta att tct gag ggc cca cta aag atg gaa gag aaa gct act tat gtt 432 Leu Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 cat aag aaa aat gat ggt aca aca gtt gat tta act gtg gat cag gca 480 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 tgg aga gga aaa ggc gaa ggt ctt cct gga atg tgt ggt ggg gcc ttg 528 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 gtt tca tcg aat caa tct ata cag aat gca atc ttg ggc atc cat gtt 576 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 gct gga gga aat tca att ctt gtt gca aaa ttg gtt act caa gaa atg 624 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 ttc caa aat att gat aag aaa att gaa agt cag 657 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 534 <211> 219 <212> PRT <213> Human hepatitus <400> 534 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 Gly Val Gly Glu Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 Arg Asp Ile Thr Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Pro 115 120 125 Leu Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 535 <211> 657 <212> DNA <213> Human hepatitus <220> <221> CDS <222> (1)..(657) <220> <221> misc_feature <222> (1)..(657) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(657) <223> Human_Hepatitis_A_Virus_NC001489_3C(T100P) <400> 535 tca act ttg gaa ata gca gga ctg gtt agg aag aac ttg gtt cag ttt 48 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 gga gtt gga gag aag aat gga tgt gtg aga tgg gtt atg aat gcc ttg 96 Gly Val Gly Glu Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 gga gtg aaa gat gat tgg ctg ctt gtg cct tcc cat gct tat aaa ttt 144 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 gag aaa gat tat gaa atg atg gag ttt tat ttt aat aga ggt gga act 192 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 tac tat tca att tca gct ggt aat gtt gtt att caa tct ttg gat gtg 240 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 gga ttc cag gat gtt gtt ctg atg aag gtt cct aca att cct aag ttt 288 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 aga gat att ccn cag cat ttt att aag aaa ggg gat gtg cct aga gct 336 Arg Asp Ile Pro Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 ttg aat cgc ctg gca aca tta gtg aca act gta aat gga acc cct atg 384 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Met 115 120 125 tta att tct gag ggc cca cta aag atg gaa gag aaa gct act tat gtt 432 Leu Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 cat aag aaa aat gat ggt aca aca gtt gat tta act gtg gat cag gca 480 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 tgg aga gga aaa ggc gaa ggt ctt cct gga atg tgt ggt ggg gcc ttg 528 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 gtt tca tcg aat caa tct ata cag aat gca atc ttg ggc atc cat gtt 576 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 gct gga gga aat tca att ctt gtt gca aaa ttg gtt act caa gaa atg 624 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 ttc caa aat att gat aag aaa att gaa agt cag 657 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 536 <211> 219 <212> PRT <213> Human hepatitus <400> 536 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 Gly Val Gly Glu Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 Arg Asp Ile Pro Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Met 115 120 125 Leu Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 537 <211> 657 <212> DNA <213> Human hepatitus <220> <221> CDS <222> (1)..(657) <220> <221> misc_feature <222> (1)..(657) <223> Human_Hepatitis_A_Virus_NC001489_3C(wt) <400> 537 tca act ttg gaa ata gca gga ctg gtt agg aag aac ttg gtt cag ttt 48 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 gga gtt gga gag aag aat gga tgt gtg aga tgg gtt atg aat gcc ttg 96 Gly Val Gly Glu Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 gga gtg aaa gat gat tgg ctg ctt gtg cct tcc cat gct tat aaa ttt 144 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 gag aaa gat tat gaa atg atg gag ttt tat ttt aat aga ggt gga act 192 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 tac tat tca att tca gct ggt aat gtt gtt att caa tct ttg gat gtg 240 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 gga ttc cag gat gtt gtt ctg atg aag gtt cct aca att cct aag ttt 288 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 aga gat att act cag cat ttt att aag aaa ggg gat gtg cct aga gct 336 Arg Asp Ile Thr Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 ttg aat cgc ctg gca aca tta gtg aca act gta aat gga acc cct atg 384 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Met 115 120 125 tta att tct gag ggc cca cta aag atg gaa gag aaa gct act tat gtt 432 Leu Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 cat aag aaa aat gat ggt aca aca gtt gat tta act gtg gat cag gca 480 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 tgg aga gga aaa ggc gaa ggt ctt cct gga atg tgt ggt ggg gcc ttg 528 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 gtt tca tcg aat caa tct ata cag aat gca atc ttg ggc atc cat gtt 576 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 gct gga gga aat tca att ctt gtt gca aaa ttg gtt act caa gaa atg 624 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 ttc caa aat att gat aag aaa att gaa agt cag 657 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 538 <211> 219 <212> PRT <213> Human hepatitus <400> 538 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 Gly Val Gly Glu Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 Arg Asp Ile Thr Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Met 115 120 125 Leu Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 539 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_2_Strain_R93152_3C(I103P) <400> 539 ggc cct ggg ttt gat tac gca gtg gcc atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca acc acc agc aaa ggg gag ttt acg atg ttg gga gtc tat gat 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val Tyr Asp 20 25 30 aat gtg gcc atc ttg cca acc cac gcc tca cct ggt gaa agc att gcg 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Ala 35 40 45 atc gac ggt aaa gag gtg gaa att ctc gac gcc aaa gcc ctt gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 cag gca gga act aat ctt gaa att acc ata att aca cta aag agg aac 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gag aag ttc aga gat atc agg cca cac att ccc acc caa atc acc gaa 288 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 aca aat gat gga gtt ttg ccn gtg aac act agt aag tac ccc aac atg 336 Thr Asn Asp Gly Val Leu Pro Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tat gtt ccc gtt ggt gct gtg acc gaa cag ggg tat ctt aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 gga cga caa acc gct cgt acg cta atg tac aac ttt cca act aga gca 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggt ggt gtc atc acg tgc act ggt aaa gtc att ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cat gtt ggt ggg aac ggt tca cat ggg ttc gcg gcg gcc cta aag cgg 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag att caa 549 Ser Tyr Phe Thr Gln Ile Gln 180 <210> 540 <211> 183 <212> PRT <213> Human poliovirus <400> 540 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val Tyr Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Ala 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Pro Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ile Gln 180 <210> 541 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_2_Strain_R93152_3C(L102P) <400> 541 ggc cct ggg ttt gat tac gca gtg gcc atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca acc acc agc aaa ggg gag ttt acg atg ttg gga gtc tat gat 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val Tyr Asp 20 25 30 aat gtg gcc atc ttg cca acc cac gcc tca cct ggt gaa agc att gcg 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Ala 35 40 45 atc gac ggt aaa gag gtg gaa att ctc gac gcc aaa gcc ctt gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 cag gca gga act aat ctt gaa att acc ata att aca cta aag agg aac 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gag aag ttc aga gat atc agg cca cac att ccc acc caa atc acc gaa 288 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 aca aat gat gga gtt ccn atc gtg aac act agt aag tac ccc aac atg 336 Thr Asn Asp Gly Val Pro Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tat gtt ccc gtt ggt gct gtg acc gaa cag ggg tat ctt aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 gga cga caa acc gct cgt acg cta atg tac aac ttt cca act aga gca 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggt ggt gtc atc acg tgc act ggt aaa gtc att ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cat gtt ggt ggg aac ggt tca cat ggg ttc gcg gcg gcc cta aag cgg 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag att caa 549 Ser Tyr Phe Thr Gln Ile Gln 180 <210> 542 <211> 183 <212> PRT <213> Human poliovirus <400> 542 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val Tyr Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Ala 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Pro Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ile Gln 180 <210> 543 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_2_Strain_R93152_3C(R87P) <400> 543 ggc cct ggg ttt gat tac gca gtg gcc atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca acc acc agc aaa ggg gag ttt acg atg ttg gga gtc tat gat 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val Tyr Asp 20 25 30 aat gtg gcc atc ttg cca acc cac gcc tca cct ggt gaa agc att gcg 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Ala 35 40 45 atc gac ggt aaa gag gtg gaa att ctc gac gcc aaa gcc ctt gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 cag gca gga act aat ctt gaa att acc ata att aca cta aag agg aac 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gag aag ttc aga gat atc ccn cca cac att ccc acc caa atc acc gaa 288 Glu Lys Phe Arg Asp Ile Pro Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 aca aat gat gga gtt ttg atc gtg aac act agt aag tac ccc aac atg 336 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tat gtt ccc gtt ggt gct gtg acc gaa cag ggg tat ctt aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 gga cga caa acc gct cgt acg cta atg tac aac ttt cca act aga gca 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggt ggt gtc atc acg tgc act ggt aaa gtc att ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cat gtt ggt ggg aac ggt tca cat ggg ttc gcg gcg gcc cta aag cgg 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag att caa 549 Ser Tyr Phe Thr Gln Ile Gln 180 <210> 544 <211> 183 <212> PRT <213> Human poliovirus <400> 544 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val Tyr Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Ala 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Pro Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ile Gln 180 <210> 545 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_2_Strain_R93152_3C(T19P) <400> 545 ggc cct ggg ttt gat tac gca gtg gcc atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca ccn acc agc aaa ggg gag ttt acg atg ttg gga gtc tat gat 96 Thr Ala Pro Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val Tyr Asp 20 25 30 aat gtg gcc atc ttg cca acc cac gcc tca cct ggt gaa agc att gcg 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Ala 35 40 45 atc gac ggt aaa gag gtg gaa att ctc gac gcc aaa gcc ctt gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 cag gca gga act aat ctt gaa att acc ata att aca cta aag agg aac 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gag aag ttc aga gat atc agg cca cac att ccc acc caa atc acc gaa 288 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 aca aat gat gga gtt ttg atc gtg aac act agt aag tac ccc aac atg 336 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tat gtt ccc gtt ggt gct gtg acc gaa cag ggg tat ctt aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 gga cga caa acc gct cgt acg cta atg tac aac ttt cca act aga gca 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggt ggt gtc atc acg tgc act ggt aaa gtc att ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cat gtt ggt ggg aac ggt tca cat ggg ttc gcg gcg gcc cta aag cgg 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag att caa 549 Ser Tyr Phe Thr Gln Ile Gln 180 <210> 546 <211> 183 <212> PRT <213> Human poliovirus <400> 546 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Pro Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val Tyr Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Ala 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ile Gln 180 <210> 547 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_2_Strain_R93152_3C(T20P) <400> 547 ggc cct ggg ttt gat tac gca gtg gcc atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca acc ccn agc aaa ggg gag ttt acg atg ttg gga gtc tat gat 96 Thr Ala Thr Pro Ser Lys Gly Glu Phe Thr Met Leu Gly Val Tyr Asp 20 25 30 aat gtg gcc atc ttg cca acc cac gcc tca cct ggt gaa agc att gcg 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Ala 35 40 45 atc gac ggt aaa gag gtg gaa att ctc gac gcc aaa gcc ctt gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 cag gca gga act aat ctt gaa att acc ata att aca cta aag agg aac 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gag aag ttc aga gat atc agg cca cac att ccc acc caa atc acc gaa 288 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 aca aat gat gga gtt ttg atc gtg aac act agt aag tac ccc aac atg 336 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tat gtt ccc gtt ggt gct gtg acc gaa cag ggg tat ctt aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 gga cga caa acc gct cgt acg cta atg tac aac ttt cca act aga gca 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggt ggt gtc atc acg tgc act ggt aaa gtc att ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cat gtt ggt ggg aac ggt tca cat ggg ttc gcg gcg gcc cta aag cgg 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag att caa 549 Ser Tyr Phe Thr Gln Ile Gln 180 <210> 548 <211> 183 <212> PRT <213> Human poliovirus <400> 548 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Pro Ser Lys Gly Glu Phe Thr Met Leu Gly Val Tyr Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Ala 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ile Gln 180 <210> 549 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_2_Strain_R93152_3C(V104P) <400> 549 ggc cct ggg ttt gat tac gca gtg gcc atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca acc acc agc aaa ggg gag ttt acg atg ttg gga gtc tat gat 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val Tyr Asp 20 25 30 aat gtg gcc atc ttg cca acc cac gcc tca cct ggt gaa agc att gcg 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Ala 35 40 45 atc gac ggt aaa gag gtg gaa att ctc gac gcc aaa gcc ctt gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 cag gca gga act aat ctt gaa att acc ata att aca cta aag agg aac 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gag aag ttc aga gat atc agg cca cac att ccc acc caa atc acc gaa 288 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 aca aat gat gga gtt ttg atc ccn aac act agt aag tac ccc aac atg 336 Thr Asn Asp Gly Val Leu Ile Pro Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tat gtt ccc gtt ggt gct gtg acc gaa cag ggg tat ctt aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 gga cga caa acc gct cgt acg cta atg tac aac ttt cca act aga gca 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggt ggt gtc atc acg tgc act ggt aaa gtc att ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cat gtt ggt ggg aac ggt tca cat ggg ttc gcg gcg gcc cta aag cgg 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag att caa 549 Ser Tyr Phe Thr Gln Ile Gln 180 <210> 550 <211> 183 <212> PRT <213> Human poliovirus <400> 550 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val Tyr Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Ala 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Pro Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ile Gln 180 <210> 551 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_2_Strain_R93152_3C(wt) <400> 551 ggc cct ggg ttt gat tac gca gtg gcc atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca acc acc agc aaa ggg gag ttt acg atg ttg gga gtc tat gat 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val Tyr Asp 20 25 30 aat gtg gcc atc ttg cca acc cac gcc tca cct ggt gaa agc att gcg 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Ala 35 40 45 atc gac ggt aaa gag gtg gaa att ctc gac gcc aaa gcc ctt gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 cag gca gga act aat ctt gaa att acc ata att aca cta aag agg aac 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gag aag ttc aga gat atc agg cca cac att ccc acc caa atc acc gaa 288 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 aca aat gat gga gtt ttg atc gtg aac act agt aag tac ccc aac atg 336 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tat gtt ccc gtt ggt gct gtg acc gaa cag ggg tat ctt aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 gga cga caa acc gct cgt acg cta atg tac aac ttt cca act aga gca 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggt ggt gtc atc acg tgc act ggt aaa gtc att ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cat gtt ggt ggg aac ggt tca cat ggg ttc gcg gcg gcc cta aag cgg 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag att caa 549 Ser Tyr Phe Thr Gln Ile Gln 180 <210> 552 <211> 183 <212> PRT <213> Human poliovirus <400> 552 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val Tyr Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Ala 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ile Gln 180 <210> 553 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_3_Isolate_CHN5275/JX/CHN/2001_FJ859183_3C(I103P) <400> 553 ggg cct ggg ttt gac tat gca gtg gct atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca act act agc aaa ggg gag ttc aca atg cta gga gtc cac gac 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aac gtg gcc att ttg cca act cat gcc tca cct ggt gag agt att gta 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 att gat ggc aaa gag gtt gaa atc cta gac gct aaa gcc ctc gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 cag gca ggc act aat ctg gaa atc acc ata ata acc ctc aaa aga aat 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gaa aag ttc aga gat atc aga caa cac ata ccc act caa atc acc gag 288 Glu Lys Phe Arg Asp Ile Arg Gln His Ile Pro Thr Gln Ile Thr Glu 85 90 95 acg aat gat gga gtt ctg ccn gtg aac act agt aag tac ccc aac atg 336 Thr Asn Asp Gly Val Leu Pro Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tac gtt cct gtc ggt gct gtg act gag cag gga tac cta aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 ggg cgc cag act gct cgt act cta atg tac aac ttt cca acc aga gct 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggt gga gtc atc aca tgc act ggg aaa gtc atc ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cac gtt ggt ggg aat ggt tca cat ggg ttt gca gcg gcc ctg aag cgg 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag agc caa 549 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 554 <211> 183 <212> PRT <213> Human poliovirus <400> 554 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gln His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Pro Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 555 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_3_Isolate_CHN5275/JX/CHN/2001_FJ859183_3C(L102P) <400> 555 ggg cct ggg ttt gac tat gca gtg gct atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca act act agc aaa ggg gag ttc aca atg cta gga gtc cac gac 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aac gtg gcc att ttg cca act cat gcc tca cct ggt gag agt att gta 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 att gat ggc aaa gag gtt gaa atc cta gac gct aaa gcc ctc gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 cag gca ggc act aat ctg gaa atc acc ata ata acc ctc aaa aga aat 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gaa aag ttc aga gat atc aga caa cac ata ccc act caa atc acc gag 288 Glu Lys Phe Arg Asp Ile Arg Gln His Ile Pro Thr Gln Ile Thr Glu 85 90 95 acg aat gat gga gtt ccn att gtg aac act agt aag tac ccc aac atg 336 Thr Asn Asp Gly Val Pro Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tac gtt cct gtc ggt gct gtg act gag cag gga tac cta aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 ggg cgc cag act gct cgt act cta atg tac aac ttt cca acc aga gct 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggt gga gtc atc aca tgc act ggg aaa gtc atc ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cac gtt ggt ggg aat ggt tca cat ggg ttt gca gcg gcc ctg aag cgg 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag agc caa 549 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 556 <211> 183 <212> PRT <213> Human poliovirus <400> 556 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gln His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Pro Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 557 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_3_Isolate_CHN5275/JX/CHN/2001_FJ859183_3C(R87P) <400> 557 ggg cct ggg ttt gac tat gca gtg gct atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca act act agc aaa ggg gag ttc aca atg cta gga gtc cac gac 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aac gtg gcc att ttg cca act cat gcc tca cct ggt gag agt att gta 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 att gat ggc aaa gag gtt gaa atc cta gac gct aaa gcc ctc gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 cag gca ggc act aat ctg gaa atc acc ata ata acc ctc aaa aga aat 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gaa aag ttc aga gat atc ccn caa cac ata ccc act caa atc acc gag 288 Glu Lys Phe Arg Asp Ile Pro Gln His Ile Pro Thr Gln Ile Thr Glu 85 90 95 acg aat gat gga gtt ctg att gtg aac act agt aag tac ccc aac atg 336 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tac gtt cct gtc ggt gct gtg act gag cag gga tac cta aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 ggg cgc cag act gct cgt act cta atg tac aac ttt cca acc aga gct 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggt gga gtc atc aca tgc act ggg aaa gtc atc ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cac gtt ggt ggg aat ggt tca cat ggg ttt gca gcg gcc ctg aag cgg 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag agc caa 549 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 558 <211> 183 <212> PRT <213> Human poliovirus <400> 558 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Pro Gln His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 559 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_3_Isolate_CHN5275/JX/CHN/2001_FJ859183_3C(T19P) <400> 559 ggg cct ggg ttt gac tat gca gtg gct atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca ccn act agc aaa ggg gag ttc aca atg cta gga gtc cac gac 96 Thr Ala Pro Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aac gtg gcc att ttg cca act cat gcc tca cct ggt gag agt att gta 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 att gat ggc aaa gag gtt gaa atc cta gac gct aaa gcc ctc gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 cag gca ggc act aat ctg gaa atc acc ata ata acc ctc aaa aga aat 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gaa aag ttc aga gat atc aga caa cac ata ccc act caa atc acc gag 288 Glu Lys Phe Arg Asp Ile Arg Gln His Ile Pro Thr Gln Ile Thr Glu 85 90 95 acg aat gat gga gtt ctg att gtg aac act agt aag tac ccc aac atg 336 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tac gtt cct gtc ggt gct gtg act gag cag gga tac cta aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 ggg cgc cag act gct cgt act cta atg tac aac ttt cca acc aga gct 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggt gga gtc atc aca tgc act ggg aaa gtc atc ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cac gtt ggt ggg aat ggt tca cat ggg ttt gca gcg gcc ctg aag cgg 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag agc caa 549 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 560 <211> 183 <212> PRT <213> Human poliovirus <400> 560 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Pro Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gln His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 561 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_3_Isolate_CHN5275/JX/CHN/2001_FJ859183_3C(T20P) <400> 561 ggg cct ggg ttt gac tat gca gtg gct atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca act ccn agc aaa ggg gag ttc aca atg cta gga gtc cac gac 96 Thr Ala Thr Pro Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aac gtg gcc att ttg cca act cat gcc tca cct ggt gag agt att gta 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 att gat ggc aaa gag gtt gaa atc cta gac gct aaa gcc ctc gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 cag gca ggc act aat ctg gaa atc acc ata ata acc ctc aaa aga aat 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gaa aag ttc aga gat atc aga caa cac ata ccc act caa atc acc gag 288 Glu Lys Phe Arg Asp Ile Arg Gln His Ile Pro Thr Gln Ile Thr Glu 85 90 95 acg aat gat gga gtt ctg att gtg aac act agt aag tac ccc aac atg 336 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tac gtt cct gtc ggt gct gtg act gag cag gga tac cta aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 ggg cgc cag act gct cgt act cta atg tac aac ttt cca acc aga gct 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggt gga gtc atc aca tgc act ggg aaa gtc atc ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cac gtt ggt ggg aat ggt tca cat ggg ttt gca gcg gcc ctg aag cgg 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag agc caa 549 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 562 <211> 183 <212> PRT <213> Human poliovirus <400> 562 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Pro Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gln His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 563 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_3_Isolate_CHN5275/JX/CHN/2001_FJ859183_3C(V104P) <400> 563 ggg cct ggg ttt gac tat gca gtg gct atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca act act agc aaa ggg gag ttc aca atg cta gga gtc cac gac 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aac gtg gcc att ttg cca act cat gcc tca cct ggt gag agt att gta 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 att gat ggc aaa gag gtt gaa atc cta gac gct aaa gcc ctc gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 cag gca ggc act aat ctg gaa atc acc ata ata acc ctc aaa aga aat 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gaa aag ttc aga gat atc aga caa cac ata ccc act caa atc acc gag 288 Glu Lys Phe Arg Asp Ile Arg Gln His Ile Pro Thr Gln Ile Thr Glu 85 90 95 acg aat gat gga gtt ctg att ccn aac act agt aag tac ccc aac atg 336 Thr Asn Asp Gly Val Leu Ile Pro Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tac gtt cct gtc ggt gct gtg act gag cag gga tac cta aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 ggg cgc cag act gct cgt act cta atg tac aac ttt cca acc aga gct 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggt gga gtc atc aca tgc act ggg aaa gtc atc ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cac gtt ggt ggg aat ggt tca cat ggg ttt gca gcg gcc ctg aag cgg 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag agc caa 549 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 564 <211> 183 <212> PRT <213> Human poliovirus <400> 564 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gln His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Pro Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 565 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_3_Isolate_CHN5275/JX/CHN/2001_FJ859183_3C(wt) <400> 565 ggg cct ggg ttt gac tat gca gtg gct atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca act act agc aaa ggg gag ttc aca atg cta gga gtc cac gac 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aac gtg gcc att ttg cca act cat gcc tca cct ggt gag agt att gta 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 att gat ggc aaa gag gtt gaa atc cta gac gct aaa gcc ctc gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 cag gca ggc act aat ctg gaa atc acc ata ata acc ctc aaa aga aat 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gaa aag ttc aga gat atc aga caa cac ata ccc act caa atc acc gag 288 Glu Lys Phe Arg Asp Ile Arg Gln His Ile Pro Thr Gln Ile Thr Glu 85 90 95 acg aat gat gga gtt ctg att gtg aac act agt aag tac ccc aac atg 336 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tac gtt cct gtc ggt gct gtg act gag cag gga tac cta aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 ggg cgc cag act gct cgt act cta atg tac aac ttt cca acc aga gct 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggt cag tgt ggt gga gtc atc aca tgc act ggg aaa gtc atc ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cac gtt ggt ggg aat ggt tca cat ggg ttt gca gcg gcc ctg aag cgg 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag agc caa 549 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 566 <211> 183 <212> PRT <213> Human poliovirus <400> 566 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gln His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 567 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_Mahoney_3C(I103P) <400> 567 gga cca ggg ttc gat tac gca gtg gct atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca act act agc aag gga gag ttc act atg tta gga gtc cac gac 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aac gtg gct att tta cca acc cac gct tca cct ggt gaa agc att gtg 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 atc gat ggc aaa gaa gtg gag atc ttg gat gcc aaa gcg ctc gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 caa gca gga acc aat ctt gaa atc act ata atc act cta aag aga aat 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gaa aag ttc aga gac att aga cca cat ata cct act caa atc act gag 288 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 aca aat gat gga gtc ttg ccn gtg aac act agc aag tac ccc aat atg 336 Thr Asn Asp Gly Val Leu Pro Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tat gtt cct gtc ggt gct gtg act gaa cag gga tat cta aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 ggg cgc caa act gct cgt act cta atg tac aac ttt cca acc aga gca 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 gga cag tgt ggt gga gtc atc aca tgt act ggg aaa gtc atc ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cat gtt ggt ggg aac ggt tca cac ggg ttt gca gcg gcc ctg aag cga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag agt caa 549 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 568 <211> 183 <212> PRT <213> Human poliovirus <400> 568 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Pro Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 569 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_Mahoney_3C(L102P) <400> 569 gga cca ggg ttc gat tac gca gtg gct atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca act act agc aag gga gag ttc act atg tta gga gtc cac gac 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aac gtg gct att tta cca acc cac gct tca cct ggt gaa agc att gtg 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 atc gat ggc aaa gaa gtg gag atc ttg gat gcc aaa gcg ctc gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 caa gca gga acc aat ctt gaa atc act ata atc act cta aag aga aat 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gaa aag ttc aga gac att aga cca cat ata cct act caa atc act gag 288 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 aca aat gat gga gtc ccn atc gtg aac act agc aag tac ccc aat atg 336 Thr Asn Asp Gly Val Pro Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tat gtt cct gtc ggt gct gtg act gaa cag gga tat cta aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 ggg cgc caa act gct cgt act cta atg tac aac ttt cca acc aga gca 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 gga cag tgt ggt gga gtc atc aca tgt act ggg aaa gtc atc ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cat gtt ggt ggg aac ggt tca cac ggg ttt gca gcg gcc ctg aag cga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag agt caa 549 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 570 <211> 183 <212> PRT <213> Human poliovirus <400> 570 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Pro Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 571 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_Mahoney_3C(R87P) <400> 571 gga cca ggg ttc gat tac gca gtg gct atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca act act agc aag gga gag ttc act atg tta gga gtc cac gac 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aac gtg gct att tta cca acc cac gct tca cct ggt gaa agc att gtg 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 atc gat ggc aaa gaa gtg gag atc ttg gat gcc aaa gcg ctc gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 caa gca gga acc aat ctt gaa atc act ata atc act cta aag aga aat 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gaa aag ttc aga gac att ccn cca cat ata cct act caa atc act gag 288 Glu Lys Phe Arg Asp Ile Pro Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 aca aat gat gga gtc ttg atc gtg aac act agc aag tac ccc aat atg 336 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tat gtt cct gtc ggt gct gtg act gaa cag gga tat cta aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 ggg cgc caa act gct cgt act cta atg tac aac ttt cca acc aga gca 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 gga cag tgt ggt gga gtc atc aca tgt act ggg aaa gtc atc ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cat gtt ggt ggg aac ggt tca cac ggg ttt gca gcg gcc ctg aag cga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag agt caa 549 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 572 <211> 183 <212> PRT <213> Human poliovirus <400> 572 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Pro Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 573 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_Mahoney_3C(T19P) <400> 573 gga cca ggg ttc gat tac gca gtg gct atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca ccn act agc aag gga gag ttc act atg tta gga gtc cac gac 96 Thr Ala Pro Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aac gtg gct att tta cca acc cac gct tca cct ggt gaa agc att gtg 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 atc gat ggc aaa gaa gtg gag atc ttg gat gcc aaa gcg ctc gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 caa gca gga acc aat ctt gaa atc act ata atc act cta aag aga aat 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gaa aag ttc aga gac att aga cca cat ata cct act caa atc act gag 288 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 aca aat gat gga gtc ttg atc gtg aac act agc aag tac ccc aat atg 336 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tat gtt cct gtc ggt gct gtg act gaa cag gga tat cta aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 ggg cgc caa act gct cgt act cta atg tac aac ttt cca acc aga gca 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 gga cag tgt ggt gga gtc atc aca tgt act ggg aaa gtc atc ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cat gtt ggt ggg aac ggt tca cac ggg ttt gca gcg gcc ctg aag cga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag agt caa 549 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 574 <211> 183 <212> PRT <213> Human poliovirus <400> 574 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Pro Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 575 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_Mahoney_3C(T20P) <400> 575 gga cca ggg ttc gat tac gca gtg gct atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca act ccn agc aag gga gag ttc act atg tta gga gtc cac gac 96 Thr Ala Thr Pro Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aac gtg gct att tta cca acc cac gct tca cct ggt gaa agc att gtg 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 atc gat ggc aaa gaa gtg gag atc ttg gat gcc aaa gcg ctc gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 caa gca gga acc aat ctt gaa atc act ata atc act cta aag aga aat 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gaa aag ttc aga gac att aga cca cat ata cct act caa atc act gag 288 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 aca aat gat gga gtc ttg atc gtg aac act agc aag tac ccc aat atg 336 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tat gtt cct gtc ggt gct gtg act gaa cag gga tat cta aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 ggg cgc caa act gct cgt act cta atg tac aac ttt cca acc aga gca 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 gga cag tgt ggt gga gtc atc aca tgt act ggg aaa gtc atc ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cat gtt ggt ggg aac ggt tca cac ggg ttt gca gcg gcc ctg aag cga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag agt caa 549 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 576 <211> 183 <212> PRT <213> Human poliovirus <400> 576 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Pro Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 577 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_Mahoney_3C(V104P) <400> 577 gga cca ggg ttc gat tac gca gtg gct atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca act act agc aag gga gag ttc act atg tta gga gtc cac gac 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aac gtg gct att tta cca acc cac gct tca cct ggt gaa agc att gtg 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 atc gat ggc aaa gaa gtg gag atc ttg gat gcc aaa gcg ctc gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 caa gca gga acc aat ctt gaa atc act ata atc act cta aag aga aat 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gaa aag ttc aga gac att aga cca cat ata cct act caa atc act gag 288 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 aca aat gat gga gtc ttg atc ccn aac act agc aag tac ccc aat atg 336 Thr Asn Asp Gly Val Leu Ile Pro Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tat gtt cct gtc ggt gct gtg act gaa cag gga tat cta aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 ggg cgc caa act gct cgt act cta atg tac aac ttt cca acc aga gca 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 gga cag tgt ggt gga gtc atc aca tgt act ggg aaa gtc atc ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cat gtt ggt ggg aac ggt tca cac ggg ttt gca gcg gcc ctg aag cga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag agt caa 549 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 578 <211> 183 <212> PRT <213> Human poliovirus <400> 578 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Pro Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 579 <211> 549 <212> DNA <213> Human poliovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> Human_Poliovirus_Mahoney_3C(wt) <400> 579 gga cca ggg ttc gat tac gca gtg gct atg gct aaa aga aac att gtt 48 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 aca gca act act agc aag gga gag ttc act atg tta gga gtc cac gac 96 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 aac gtg gct att tta cca acc cac gct tca cct ggt gaa agc att gtg 144 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 atc gat ggc aaa gaa gtg gag atc ttg gat gcc aaa gcg ctc gaa gat 192 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 caa gca gga acc aat ctt gaa atc act ata atc act cta aag aga aat 240 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 gaa aag ttc aga gac att aga cca cat ata cct act caa atc act gag 288 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 aca aat gat gga gtc ttg atc gtg aac act agc aag tac ccc aat atg 336 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 tat gtt cct gtc ggt gct gtg act gaa cag gga tat cta aat ctc ggt 384 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 ggg cgc caa act gct cgt act cta atg tac aac ttt cca acc aga gca 432 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 gga cag tgt ggt gga gtc atc aca tgt act ggg aaa gtc atc ggg atg 480 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 cat gtt ggt ggg aac ggt tca cac ggg ttt gca gcg gcc ctg aag cga 528 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 tca tac ttc act cag agt caa 549 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 580 <211> 183 <212> PRT <213> Human poliovirus <400> 580 Gly Pro Gly Phe Asp Tyr Ala Val Ala Met Ala Lys Arg Asn Ile Val 1 5 10 15 Thr Ala Thr Thr Ser Lys Gly Glu Phe Thr Met Leu Gly Val His Asp 20 25 30 Asn Val Ala Ile Leu Pro Thr His Ala Ser Pro Gly Glu Ser Ile Val 35 40 45 Ile Asp Gly Lys Glu Val Glu Ile Leu Asp Ala Lys Ala Leu Glu Asp 50 55 60 Gln Ala Gly Thr Asn Leu Glu Ile Thr Ile Ile Thr Leu Lys Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro His Ile Pro Thr Gln Ile Thr Glu 85 90 95 Thr Asn Asp Gly Val Leu Ile Val Asn Thr Ser Lys Tyr Pro Asn Met 100 105 110 Tyr Val Pro Val Gly Ala Val Thr Glu Gln Gly Tyr Leu Asn Leu Gly 115 120 125 Gly Arg Gln Thr Ala Arg Thr Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Ile Thr Cys Thr Gly Lys Val Ile Gly Met 145 150 155 160 His Val Gly Gly Asn Gly Ser His Gly Phe Ala Ala Ala Leu Lys Arg 165 170 175 Ser Tyr Phe Thr Gln Ser Gln 180 <210> 581 <211> 546 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(546) <220> <221> misc_feature <222> (1)..(546) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(546) <223> Human_Rhinovirus_14_NC001490_3C(E114P) <400> 581 gga cca aac aca gaa ttt gca cta tcc ctg tta agg aaa aac ata atg 48 Gly Pro Asn Thr Glu Phe Ala Leu Ser Leu Leu Arg Lys Asn Ile Met 1 5 10 15 act ata aca acc tca aag gga gag ttc aca ggg tta ggc ata cat gat 96 Thr Ile Thr Thr Ser Lys Gly Glu Phe Thr Gly Leu Gly Ile His Asp 20 25 30 cgt gtc tgt gtg ata ccc aca cac gca cag cct ggt gat gat gta cta 144 Arg Val Cys Val Ile Pro Thr His Ala Gln Pro Gly Asp Asp Val Leu 35 40 45 gtg aat ggt cag aaa att aga gtt aag gat aag tac aaa tta gta gat 192 Val Asn Gly Gln Lys Ile Arg Val Lys Asp Lys Tyr Lys Leu Val Asp 50 55 60 cca gag aac att aat cta gag ctt aca gtg ttg act tta gat aga aat 240 Pro Glu Asn Ile Asn Leu Glu Leu Thr Val Leu Thr Leu Asp Arg Asn 65 70 75 80 gaa aaa ttc aga gat atc agg gga ttt ata tca gaa gat cta gaa ggt 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Ile Ser Glu Asp Leu Glu Gly 85 90 95 gtg gat gcc act ttg gta gta cat tca aat aac ttt acc aac act atc 336 Val Asp Ala Thr Leu Val Val His Ser Asn Asn Phe Thr Asn Thr Ile 100 105 110 tta ccn gtt ggc cct gta aca atg gca gga ctt att aat ttg agt agc 384 Leu Pro Val Gly Pro Val Thr Met Ala Gly Leu Ile Asn Leu Ser Ser 115 120 125 acc ccc act aac aga atg att cgt tat gat tat gca aca aaa act ggg 432 Thr Pro Thr Asn Arg Met Ile Arg Tyr Asp Tyr Ala Thr Lys Thr Gly 130 135 140 cag tgt gga ggt gtg ctg tgt gct act ggt aag atc ttt ggt att cat 480 Gln Cys Gly Gly Val Leu Cys Ala Thr Gly Lys Ile Phe Gly Ile His 145 150 155 160 gtt ggc ggt aat gga aga caa gga ttt tca gct caa ctt aaa aaa caa 528 Val Gly Gly Asn Gly Arg Gln Gly Phe Ser Ala Gln Leu Lys Lys Gln 165 170 175 tat ttt gta gag aaa caa 546 Tyr Phe Val Glu Lys Gln 180 <210> 582 <211> 182 <212> PRT <213> Human rhinovirus <400> 582 Gly Pro Asn Thr Glu Phe Ala Leu Ser Leu Leu Arg Lys Asn Ile Met 1 5 10 15 Thr Ile Thr Thr Ser Lys Gly Glu Phe Thr Gly Leu Gly Ile His Asp 20 25 30 Arg Val Cys Val Ile Pro Thr His Ala Gln Pro Gly Asp Asp Val Leu 35 40 45 Val Asn Gly Gln Lys Ile Arg Val Lys Asp Lys Tyr Lys Leu Val Asp 50 55 60 Pro Glu Asn Ile Asn Leu Glu Leu Thr Val Leu Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Ile Ser Glu Asp Leu Glu Gly 85 90 95 Val Asp Ala Thr Leu Val Val His Ser Asn Asn Phe Thr Asn Thr Ile 100 105 110 Leu Pro Val Gly Pro Val Thr Met Ala Gly Leu Ile Asn Leu Ser Ser 115 120 125 Thr Pro Thr Asn Arg Met Ile Arg Tyr Asp Tyr Ala Thr Lys Thr Gly 130 135 140 Gln Cys Gly Gly Val Leu Cys Ala Thr Gly Lys Ile Phe Gly Ile His 145 150 155 160 Val Gly Gly Asn Gly Arg Gln Gly Phe Ser Ala Gln Leu Lys Lys Gln 165 170 175 Tyr Phe Val Glu Lys Gln 180 <210> 583 <211> 546 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(546) <220> <221> misc_feature <222> (1)..(546) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(546) <223> Human_Rhinovirus_14_NC001490_3C(G88P) <400> 583 gga cca aac aca gaa ttt gca cta tcc ctg tta agg aaa aac ata atg 48 Gly Pro Asn Thr Glu Phe Ala Leu Ser Leu Leu Arg Lys Asn Ile Met 1 5 10 15 act ata aca acc tca aag gga gag ttc aca ggg tta ggc ata cat gat 96 Thr Ile Thr Thr Ser Lys Gly Glu Phe Thr Gly Leu Gly Ile His Asp 20 25 30 cgt gtc tgt gtg ata ccc aca cac gca cag cct ggt gat gat gta cta 144 Arg Val Cys Val Ile Pro Thr His Ala Gln Pro Gly Asp Asp Val Leu 35 40 45 gtg aat ggt cag aaa att aga gtt aag gat aag tac aaa tta gta gat 192 Val Asn Gly Gln Lys Ile Arg Val Lys Asp Lys Tyr Lys Leu Val Asp 50 55 60 cca gag aac att aat cta gag ctt aca gtg ttg act tta gat aga aat 240 Pro Glu Asn Ile Asn Leu Glu Leu Thr Val Leu Thr Leu Asp Arg Asn 65 70 75 80 gaa aaa ttc aga gat atc agg ccn ttt ata tca gaa gat cta gaa ggt 288 Glu Lys Phe Arg Asp Ile Arg Pro Phe Ile Ser Glu Asp Leu Glu Gly 85 90 95 gtg gat gcc act ttg gta gta cat tca aat aac ttt acc aac act atc 336 Val Asp Ala Thr Leu Val Val His Ser Asn Asn Phe Thr Asn Thr Ile 100 105 110 tta gaa gtt ggc cct gta aca atg gca gga ctt att aat ttg agt agc 384 Leu Glu Val Gly Pro Val Thr Met Ala Gly Leu Ile Asn Leu Ser Ser 115 120 125 acc ccc act aac aga atg att cgt tat gat tat gca aca aaa act ggg 432 Thr Pro Thr Asn Arg Met Ile Arg Tyr Asp Tyr Ala Thr Lys Thr Gly 130 135 140 cag tgt gga ggt gtg ctg tgt gct act ggt aag atc ttt ggt att cat 480 Gln Cys Gly Gly Val Leu Cys Ala Thr Gly Lys Ile Phe Gly Ile His 145 150 155 160 gtt ggc ggt aat gga aga caa gga ttt tca gct caa ctt aaa aaa caa 528 Val Gly Gly Asn Gly Arg Gln Gly Phe Ser Ala Gln Leu Lys Lys Gln 165 170 175 tat ttt gta gag aaa caa 546 Tyr Phe Val Glu Lys Gln 180 <210> 584 <211> 182 <212> PRT <213> Human rhinovirus <400> 584 Gly Pro Asn Thr Glu Phe Ala Leu Ser Leu Leu Arg Lys Asn Ile Met 1 5 10 15 Thr Ile Thr Thr Ser Lys Gly Glu Phe Thr Gly Leu Gly Ile His Asp 20 25 30 Arg Val Cys Val Ile Pro Thr His Ala Gln Pro Gly Asp Asp Val Leu 35 40 45 Val Asn Gly Gln Lys Ile Arg Val Lys Asp Lys Tyr Lys Leu Val Asp 50 55 60 Pro Glu Asn Ile Asn Leu Glu Leu Thr Val Leu Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro Phe Ile Ser Glu Asp Leu Glu Gly 85 90 95 Val Asp Ala Thr Leu Val Val His Ser Asn Asn Phe Thr Asn Thr Ile 100 105 110 Leu Glu Val Gly Pro Val Thr Met Ala Gly Leu Ile Asn Leu Ser Ser 115 120 125 Thr Pro Thr Asn Arg Met Ile Arg Tyr Asp Tyr Ala Thr Lys Thr Gly 130 135 140 Gln Cys Gly Gly Val Leu Cys Ala Thr Gly Lys Ile Phe Gly Ile His 145 150 155 160 Val Gly Gly Asn Gly Arg Gln Gly Phe Ser Ala Gln Leu Lys Lys Gln 165 170 175 Tyr Phe Val Glu Lys Gln 180 <210> 585 <211> 546 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(546) <220> <221> misc_feature <222> (1)..(546) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(546) <223> Human_Rhinovirus_14_NC001490_3C(I112P) <400> 585 gga cca aac aca gaa ttt gca cta tcc ctg tta agg aaa aac ata atg 48 Gly Pro Asn Thr Glu Phe Ala Leu Ser Leu Leu Arg Lys Asn Ile Met 1 5 10 15 act ata aca acc tca aag gga gag ttc aca ggg tta ggc ata cat gat 96 Thr Ile Thr Thr Ser Lys Gly Glu Phe Thr Gly Leu Gly Ile His Asp 20 25 30 cgt gtc tgt gtg ata ccc aca cac gca cag cct ggt gat gat gta cta 144 Arg Val Cys Val Ile Pro Thr His Ala Gln Pro Gly Asp Asp Val Leu 35 40 45 gtg aat ggt cag aaa att aga gtt aag gat aag tac aaa tta gta gat 192 Val Asn Gly Gln Lys Ile Arg Val Lys Asp Lys Tyr Lys Leu Val Asp 50 55 60 cca gag aac att aat cta gag ctt aca gtg ttg act tta gat aga aat 240 Pro Glu Asn Ile Asn Leu Glu Leu Thr Val Leu Thr Leu Asp Arg Asn 65 70 75 80 gaa aaa ttc aga gat atc agg gga ttt ata tca gaa gat cta gaa ggt 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Ile Ser Glu Asp Leu Glu Gly 85 90 95 gtg gat gcc act ttg gta gta cat tca aat aac ttt acc aac act ccn 336 Val Asp Ala Thr Leu Val Val His Ser Asn Asn Phe Thr Asn Thr Pro 100 105 110 tta gaa gtt ggc cct gta aca atg gca gga ctt att aat ttg agt agc 384 Leu Glu Val Gly Pro Val Thr Met Ala Gly Leu Ile Asn Leu Ser Ser 115 120 125 acc ccc act aac aga atg att cgt tat gat tat gca aca aaa act ggg 432 Thr Pro Thr Asn Arg Met Ile Arg Tyr Asp Tyr Ala Thr Lys Thr Gly 130 135 140 cag tgt gga ggt gtg ctg tgt gct act ggt aag atc ttt ggt att cat 480 Gln Cys Gly Gly Val Leu Cys Ala Thr Gly Lys Ile Phe Gly Ile His 145 150 155 160 gtt ggc ggt aat gga aga caa gga ttt tca gct caa ctt aaa aaa caa 528 Val Gly Gly Asn Gly Arg Gln Gly Phe Ser Ala Gln Leu Lys Lys Gln 165 170 175 tat ttt gta gag aaa caa 546 Tyr Phe Val Glu Lys Gln 180 <210> 586 <211> 182 <212> PRT <213> Human rhinovirus <400> 586 Gly Pro Asn Thr Glu Phe Ala Leu Ser Leu Leu Arg Lys Asn Ile Met 1 5 10 15 Thr Ile Thr Thr Ser Lys Gly Glu Phe Thr Gly Leu Gly Ile His Asp 20 25 30 Arg Val Cys Val Ile Pro Thr His Ala Gln Pro Gly Asp Asp Val Leu 35 40 45 Val Asn Gly Gln Lys Ile Arg Val Lys Asp Lys Tyr Lys Leu Val Asp 50 55 60 Pro Glu Asn Ile Asn Leu Glu Leu Thr Val Leu Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Ile Ser Glu Asp Leu Glu Gly 85 90 95 Val Asp Ala Thr Leu Val Val His Ser Asn Asn Phe Thr Asn Thr Pro 100 105 110 Leu Glu Val Gly Pro Val Thr Met Ala Gly Leu Ile Asn Leu Ser Ser 115 120 125 Thr Pro Thr Asn Arg Met Ile Arg Tyr Asp Tyr Ala Thr Lys Thr Gly 130 135 140 Gln Cys Gly Gly Val Leu Cys Ala Thr Gly Lys Ile Phe Gly Ile His 145 150 155 160 Val Gly Gly Asn Gly Arg Gln Gly Phe Ser Ala Gln Leu Lys Lys Gln 165 170 175 Tyr Phe Val Glu Lys Gln 180 <210> 587 <211> 546 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(546) <220> <221> misc_feature <222> (1)..(546) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(546) <223> Human_Rhinovirus_14_NC001490(R87P) <400> 587 gga cca aac aca gaa ttt gca cta tcc ctg tta agg aaa aac ata atg 48 Gly Pro Asn Thr Glu Phe Ala Leu Ser Leu Leu Arg Lys Asn Ile Met 1 5 10 15 act ata aca acc tca aag gga gag ttc aca ggg tta ggc ata cat gat 96 Thr Ile Thr Thr Ser Lys Gly Glu Phe Thr Gly Leu Gly Ile His Asp 20 25 30 cgt gtc tgt gtg ata ccc aca cac gca cag cct ggt gat gat gta cta 144 Arg Val Cys Val Ile Pro Thr His Ala Gln Pro Gly Asp Asp Val Leu 35 40 45 gtg aat ggt cag aaa att aga gtt aag gat aag tac aaa tta gta gat 192 Val Asn Gly Gln Lys Ile Arg Val Lys Asp Lys Tyr Lys Leu Val Asp 50 55 60 cca gag aac att aat cta gag ctt aca gtg ttg act tta gat aga aat 240 Pro Glu Asn Ile Asn Leu Glu Leu Thr Val Leu Thr Leu Asp Arg Asn 65 70 75 80 gaa aaa ttc aga gat atc ccn gga ttt ata tca gaa gat cta gaa ggt 288 Glu Lys Phe Arg Asp Ile Pro Gly Phe Ile Ser Glu Asp Leu Glu Gly 85 90 95 gtg gat gcc act ttg gta gta cat tca aat aac ttt acc aac act atc 336 Val Asp Ala Thr Leu Val Val His Ser Asn Asn Phe Thr Asn Thr Ile 100 105 110 tta gaa gtt ggc cct gta aca atg gca gga ctt att aat ttg agt agc 384 Leu Glu Val Gly Pro Val Thr Met Ala Gly Leu Ile Asn Leu Ser Ser 115 120 125 acc ccc act aac aga atg att cgt tat gat tat gca aca aaa act ggg 432 Thr Pro Thr Asn Arg Met Ile Arg Tyr Asp Tyr Ala Thr Lys Thr Gly 130 135 140 cag tgt gga ggt gtg ctg tgt gct act ggt aag atc ttt ggt att cat 480 Gln Cys Gly Gly Val Leu Cys Ala Thr Gly Lys Ile Phe Gly Ile His 145 150 155 160 gtt ggc ggt aat gga aga caa gga ttt tca gct caa ctt aaa aaa caa 528 Val Gly Gly Asn Gly Arg Gln Gly Phe Ser Ala Gln Leu Lys Lys Gln 165 170 175 tat ttt gta gag aaa caa 546 Tyr Phe Val Glu Lys Gln 180 <210> 588 <211> 182 <212> PRT <213> Human rhinovirus <400> 588 Gly Pro Asn Thr Glu Phe Ala Leu Ser Leu Leu Arg Lys Asn Ile Met 1 5 10 15 Thr Ile Thr Thr Ser Lys Gly Glu Phe Thr Gly Leu Gly Ile His Asp 20 25 30 Arg Val Cys Val Ile Pro Thr His Ala Gln Pro Gly Asp Asp Val Leu 35 40 45 Val Asn Gly Gln Lys Ile Arg Val Lys Asp Lys Tyr Lys Leu Val Asp 50 55 60 Pro Glu Asn Ile Asn Leu Glu Leu Thr Val Leu Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Pro Gly Phe Ile Ser Glu Asp Leu Glu Gly 85 90 95 Val Asp Ala Thr Leu Val Val His Ser Asn Asn Phe Thr Asn Thr Ile 100 105 110 Leu Glu Val Gly Pro Val Thr Met Ala Gly Leu Ile Asn Leu Ser Ser 115 120 125 Thr Pro Thr Asn Arg Met Ile Arg Tyr Asp Tyr Ala Thr Lys Thr Gly 130 135 140 Gln Cys Gly Gly Val Leu Cys Ala Thr Gly Lys Ile Phe Gly Ile His 145 150 155 160 Val Gly Gly Asn Gly Arg Gln Gly Phe Ser Ala Gln Leu Lys Lys Gln 165 170 175 Tyr Phe Val Glu Lys Gln 180 <210> 589 <211> 546 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(546) <220> <221> misc_feature <222> (1)..(546) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(546) <223> Human_Rhinovirus_14_NC001490_3C(T19P) <400> 589 gga cca aac aca gaa ttt gca cta tcc ctg tta agg aaa aac ata atg 48 Gly Pro Asn Thr Glu Phe Ala Leu Ser Leu Leu Arg Lys Asn Ile Met 1 5 10 15 act ata ccn acc tca aag gga gag ttc aca ggg tta ggc ata cat gat 96 Thr Ile Pro Thr Ser Lys Gly Glu Phe Thr Gly Leu Gly Ile His Asp 20 25 30 cgt gtc tgt gtg ata ccc aca cac gca cag cct ggt gat gat gta cta 144 Arg Val Cys Val Ile Pro Thr His Ala Gln Pro Gly Asp Asp Val Leu 35 40 45 gtg aat ggt cag aaa att aga gtt aag gat aag tac aaa tta gta gat 192 Val Asn Gly Gln Lys Ile Arg Val Lys Asp Lys Tyr Lys Leu Val Asp 50 55 60 cca gag aac att aat cta gag ctt aca gtg ttg act tta gat aga aat 240 Pro Glu Asn Ile Asn Leu Glu Leu Thr Val Leu Thr Leu Asp Arg Asn 65 70 75 80 gaa aaa ttc aga gat atc agg gga ttt ata tca gaa gat cta gaa ggt 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Ile Ser Glu Asp Leu Glu Gly 85 90 95 gtg gat gcc act ttg gta gta cat tca aat aac ttt acc aac act atc 336 Val Asp Ala Thr Leu Val Val His Ser Asn Asn Phe Thr Asn Thr Ile 100 105 110 tta gaa gtt ggc cct gta aca atg gca gga ctt att aat ttg agt agc 384 Leu Glu Val Gly Pro Val Thr Met Ala Gly Leu Ile Asn Leu Ser Ser 115 120 125 acc ccc act aac aga atg att cgt tat gat tat gca aca aaa act ggg 432 Thr Pro Thr Asn Arg Met Ile Arg Tyr Asp Tyr Ala Thr Lys Thr Gly 130 135 140 cag tgt gga ggt gtg ctg tgt gct act ggt aag atc ttt ggt att cat 480 Gln Cys Gly Gly Val Leu Cys Ala Thr Gly Lys Ile Phe Gly Ile His 145 150 155 160 gtt ggc ggt aat gga aga caa gga ttt tca gct caa ctt aaa aaa caa 528 Val Gly Gly Asn Gly Arg Gln Gly Phe Ser Ala Gln Leu Lys Lys Gln 165 170 175 tat ttt gta gag aaa caa 546 Tyr Phe Val Glu Lys Gln 180 <210> 590 <211> 182 <212> PRT <213> Human rhinovirus <400> 590 Gly Pro Asn Thr Glu Phe Ala Leu Ser Leu Leu Arg Lys Asn Ile Met 1 5 10 15 Thr Ile Pro Thr Ser Lys Gly Glu Phe Thr Gly Leu Gly Ile His Asp 20 25 30 Arg Val Cys Val Ile Pro Thr His Ala Gln Pro Gly Asp Asp Val Leu 35 40 45 Val Asn Gly Gln Lys Ile Arg Val Lys Asp Lys Tyr Lys Leu Val Asp 50 55 60 Pro Glu Asn Ile Asn Leu Glu Leu Thr Val Leu Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Ile Ser Glu Asp Leu Glu Gly 85 90 95 Val Asp Ala Thr Leu Val Val His Ser Asn Asn Phe Thr Asn Thr Ile 100 105 110 Leu Glu Val Gly Pro Val Thr Met Ala Gly Leu Ile Asn Leu Ser Ser 115 120 125 Thr Pro Thr Asn Arg Met Ile Arg Tyr Asp Tyr Ala Thr Lys Thr Gly 130 135 140 Gln Cys Gly Gly Val Leu Cys Ala Thr Gly Lys Ile Phe Gly Ile His 145 150 155 160 Val Gly Gly Asn Gly Arg Gln Gly Phe Ser Ala Gln Leu Lys Lys Gln 165 170 175 Tyr Phe Val Glu Lys Gln 180 <210> 591 <211> 546 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(546) <220> <221> misc_feature <222> (1)..(546) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(546) <223> Human_Rhinovirus_14_NC001490_3C(T20P) <400> 591 gga cca aac aca gaa ttt gca cta tcc ctg tta agg aaa aac ata atg 48 Gly Pro Asn Thr Glu Phe Ala Leu Ser Leu Leu Arg Lys Asn Ile Met 1 5 10 15 act ata aca ccn tca aag gga gag ttc aca ggg tta ggc ata cat gat 96 Thr Ile Thr Pro Ser Lys Gly Glu Phe Thr Gly Leu Gly Ile His Asp 20 25 30 cgt gtc tgt gtg ata ccc aca cac gca cag cct ggt gat gat gta cta 144 Arg Val Cys Val Ile Pro Thr His Ala Gln Pro Gly Asp Asp Val Leu 35 40 45 gtg aat ggt cag aaa att aga gtt aag gat aag tac aaa tta gta gat 192 Val Asn Gly Gln Lys Ile Arg Val Lys Asp Lys Tyr Lys Leu Val Asp 50 55 60 cca gag aac att aat cta gag ctt aca gtg ttg act tta gat aga aat 240 Pro Glu Asn Ile Asn Leu Glu Leu Thr Val Leu Thr Leu Asp Arg Asn 65 70 75 80 gaa aaa ttc aga gat atc agg gga ttt ata tca gaa gat cta gaa ggt 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Ile Ser Glu Asp Leu Glu Gly 85 90 95 gtg gat gcc act ttg gta gta cat tca aat aac ttt acc aac act atc 336 Val Asp Ala Thr Leu Val Val His Ser Asn Asn Phe Thr Asn Thr Ile 100 105 110 tta gaa gtt ggc cct gta aca atg gca gga ctt att aat ttg agt agc 384 Leu Glu Val Gly Pro Val Thr Met Ala Gly Leu Ile Asn Leu Ser Ser 115 120 125 acc ccc act aac aga atg att cgt tat gat tat gca aca aaa act ggg 432 Thr Pro Thr Asn Arg Met Ile Arg Tyr Asp Tyr Ala Thr Lys Thr Gly 130 135 140 cag tgt gga ggt gtg ctg tgt gct act ggt aag atc ttt ggt att cat 480 Gln Cys Gly Gly Val Leu Cys Ala Thr Gly Lys Ile Phe Gly Ile His 145 150 155 160 gtt ggc ggt aat gga aga caa gga ttt tca gct caa ctt aaa aaa caa 528 Val Gly Gly Asn Gly Arg Gln Gly Phe Ser Ala Gln Leu Lys Lys Gln 165 170 175 tat ttt gta gag aaa caa 546 Tyr Phe Val Glu Lys Gln 180 <210> 592 <211> 182 <212> PRT <213> Human rhinovirus <400> 592 Gly Pro Asn Thr Glu Phe Ala Leu Ser Leu Leu Arg Lys Asn Ile Met 1 5 10 15 Thr Ile Thr Pro Ser Lys Gly Glu Phe Thr Gly Leu Gly Ile His Asp 20 25 30 Arg Val Cys Val Ile Pro Thr His Ala Gln Pro Gly Asp Asp Val Leu 35 40 45 Val Asn Gly Gln Lys Ile Arg Val Lys Asp Lys Tyr Lys Leu Val Asp 50 55 60 Pro Glu Asn Ile Asn Leu Glu Leu Thr Val Leu Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Ile Ser Glu Asp Leu Glu Gly 85 90 95 Val Asp Ala Thr Leu Val Val His Ser Asn Asn Phe Thr Asn Thr Ile 100 105 110 Leu Glu Val Gly Pro Val Thr Met Ala Gly Leu Ile Asn Leu Ser Ser 115 120 125 Thr Pro Thr Asn Arg Met Ile Arg Tyr Asp Tyr Ala Thr Lys Thr Gly 130 135 140 Gln Cys Gly Gly Val Leu Cys Ala Thr Gly Lys Ile Phe Gly Ile His 145 150 155 160 Val Gly Gly Asn Gly Arg Gln Gly Phe Ser Ala Gln Leu Lys Lys Gln 165 170 175 Tyr Phe Val Glu Lys Gln 180 <210> 593 <211> 546 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(546) <220> <221> misc_feature <222> (1)..(546) <223> Human_Rhinovirus_14_NC001490_3C(wt) <400> 593 gga cca aac aca gaa ttt gca cta tcc ctg tta agg aaa aac ata atg 48 Gly Pro Asn Thr Glu Phe Ala Leu Ser Leu Leu Arg Lys Asn Ile Met 1 5 10 15 act ata aca acc tca aag gga gag ttc aca ggg tta ggc ata cat gat 96 Thr Ile Thr Thr Ser Lys Gly Glu Phe Thr Gly Leu Gly Ile His Asp 20 25 30 cgt gtc tgt gtg ata ccc aca cac gca cag cct ggt gat gat gta cta 144 Arg Val Cys Val Ile Pro Thr His Ala Gln Pro Gly Asp Asp Val Leu 35 40 45 gtg aat ggt cag aaa att aga gtt aag gat aag tac aaa tta gta gat 192 Val Asn Gly Gln Lys Ile Arg Val Lys Asp Lys Tyr Lys Leu Val Asp 50 55 60 cca gag aac att aat cta gag ctt aca gtg ttg act tta gat aga aat 240 Pro Glu Asn Ile Asn Leu Glu Leu Thr Val Leu Thr Leu Asp Arg Asn 65 70 75 80 gaa aaa ttc aga gat atc agg gga ttt ata tca gaa gat cta gaa ggt 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Ile Ser Glu Asp Leu Glu Gly 85 90 95 gtg gat gcc act ttg gta gta cat tca aat aac ttt acc aac act atc 336 Val Asp Ala Thr Leu Val Val His Ser Asn Asn Phe Thr Asn Thr Ile 100 105 110 tta gaa gtt ggc cct gta aca atg gca gga ctt att aat ttg agt agc 384 Leu Glu Val Gly Pro Val Thr Met Ala Gly Leu Ile Asn Leu Ser Ser 115 120 125 acc ccc act aac aga atg att cgt tat gat tat gca aca aaa act ggg 432 Thr Pro Thr Asn Arg Met Ile Arg Tyr Asp Tyr Ala Thr Lys Thr Gly 130 135 140 cag tgt gga ggt gtg ctg tgt gct act ggt aag atc ttt ggt att cat 480 Gln Cys Gly Gly Val Leu Cys Ala Thr Gly Lys Ile Phe Gly Ile His 145 150 155 160 gtt ggc ggt aat gga aga caa gga ttt tca gct caa ctt aaa aaa caa 528 Val Gly Gly Asn Gly Arg Gln Gly Phe Ser Ala Gln Leu Lys Lys Gln 165 170 175 tat ttt gta gag aaa caa 546 Tyr Phe Val Glu Lys Gln 180 <210> 594 <211> 182 <212> PRT <213> Human rhinovirus <400> 594 Gly Pro Asn Thr Glu Phe Ala Leu Ser Leu Leu Arg Lys Asn Ile Met 1 5 10 15 Thr Ile Thr Thr Ser Lys Gly Glu Phe Thr Gly Leu Gly Ile His Asp 20 25 30 Arg Val Cys Val Ile Pro Thr His Ala Gln Pro Gly Asp Asp Val Leu 35 40 45 Val Asn Gly Gln Lys Ile Arg Val Lys Asp Lys Tyr Lys Leu Val Asp 50 55 60 Pro Glu Asn Ile Asn Leu Glu Leu Thr Val Leu Thr Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Ile Ser Glu Asp Leu Glu Gly 85 90 95 Val Asp Ala Thr Leu Val Val His Ser Asn Asn Phe Thr Asn Thr Ile 100 105 110 Leu Glu Val Gly Pro Val Thr Met Ala Gly Leu Ile Asn Leu Ser Ser 115 120 125 Thr Pro Thr Asn Arg Met Ile Arg Tyr Asp Tyr Ala Thr Lys Thr Gly 130 135 140 Gln Cys Gly Gly Val Leu Cys Ala Thr Gly Lys Ile Phe Gly Ile His 145 150 155 160 Val Gly Gly Asn Gly Arg Gln Gly Phe Ser Ala Gln Leu Lys Lys Gln 165 170 175 Tyr Phe Val Glu Lys Gln 180 <210> 595 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(546) <220> <221> misc_feature <222> (1)..(546) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(546) <223> Human_Rhinovirus_20_Strain_ATCC_VR1130_3C(K88P) <400> 595 ggg cca caa gaa gaa ttt gga cgt agt tta atc aaa cat aac act tgt 48 Gly Pro Gln Glu Glu Phe Gly Arg Ser Leu Ile Lys His Asn Thr Cys 1 5 10 15 gtt gta aca aca gac aat ggt aag ttc act gga tta ggg atc tat gac 96 Val Val Thr Thr Asp Asn Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 aat ata atg att ata cca aca cat gct gat gct ggg aaa gaa gta gaa 144 Asn Ile Met Ile Ile Pro Thr His Ala Asp Ala Gly Lys Glu Val Glu 35 40 45 ata gat ggg atc aaa act cag gtt gat gat gct tat gat tta cat aat 192 Ile Asp Gly Ile Lys Thr Gln Val Asp Asp Ala Tyr Asp Leu His Asn 50 55 60 tcc cag gga atc aaa ttg gaa att aca gtc ctt aaa ttg cat aga aat 240 Ser Gln Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu His Arg Asn 65 70 75 80 gaa aag ttt aga gac att aga ccn tac ata cca gag act gaa gat gat 288 Glu Lys Phe Arg Asp Ile Arg Pro Tyr Ile Pro Glu Thr Glu Asp Asp 85 90 95 tat cca gaa tgc cat tta gca ctg gtt gct aat caa cag gag cct act 336 Tyr Pro Glu Cys His Leu Ala Leu Val Ala Asn Gln Gln Glu Pro Thr 100 105 110 ata cta gaa gtt ggc gac tgc tgc tca tat gga aac ata tta tta agt 384 Ile Leu Glu Val Gly Asp Cys Cys Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggc aac cag aca gct aga atg att aaa tac aac tac cca aca aaa tca 432 Gly Asn Gln Thr Ala Arg Met Ile Lys Tyr Asn Tyr Pro Thr Lys Ser 130 135 140 gga ttc tgt gga ggg gtc ttg tat aaa ata gga tta gtg cta ggt ata 480 Gly Phe Cys Gly Gly Val Leu Tyr Lys Ile Gly Leu Val Leu Gly Ile 145 150 155 160 cat gtt ggg gga aat ggt agg gat ggt ttt tcc gcg atg cta cta aga 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 tca tat ttc act gaa caa cag 549 Ser Tyr Phe Thr Glu Gln 180 <210> 596 <211> 182 <212> PRT <213> Human rhinovirus <400> 596 Gly Pro Gln Glu Glu Phe Gly Arg Ser Leu Ile Lys His Asn Thr Cys 1 5 10 15 Val Val Thr Thr Asp Asn Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 Asn Ile Met Ile Ile Pro Thr His Ala Asp Ala Gly Lys Glu Val Glu 35 40 45 Ile Asp Gly Ile Lys Thr Gln Val Asp Asp Ala Tyr Asp Leu His Asn 50 55 60 Ser Gln Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu His Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro Tyr Ile Pro Glu Thr Glu Asp Asp 85 90 95 Tyr Pro Glu Cys His Leu Ala Leu Val Ala Asn Gln Gln Glu Pro Thr 100 105 110 Ile Leu Glu Val Gly Asp Cys Cys Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Asn Gln Thr Ala Arg Met Ile Lys Tyr Asn Tyr Pro Thr Lys Ser 130 135 140 Gly Phe Cys Gly Gly Val Leu Tyr Lys Ile Gly Leu Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 Ser Tyr Phe Thr Glu Gln 180 <210> 597 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_20_Strain_ATCC_VR1130_3C(R87P) <400> 597 ggg cca caa gaa gaa ttt gga cgt agt tta atc aaa cat aac act tgt 48 Gly Pro Gln Glu Glu Phe Gly Arg Ser Leu Ile Lys His Asn Thr Cys 1 5 10 15 gtt gta aca aca gac aat ggt aag ttc act gga tta ggg atc tat gac 96 Val Val Thr Thr Asp Asn Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 aat ata atg att ata cca aca cat gct gat gct ggg aaa gaa gta gaa 144 Asn Ile Met Ile Ile Pro Thr His Ala Asp Ala Gly Lys Glu Val Glu 35 40 45 ata gat ggg atc aaa act cag gtt gat gat gct tat gat tta cat aat 192 Ile Asp Gly Ile Lys Thr Gln Val Asp Asp Ala Tyr Asp Leu His Asn 50 55 60 tcc cag gga atc aaa ttg gaa att aca gtc ctt aaa ttg cat aga aat 240 Ser Gln Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu His Arg Asn 65 70 75 80 gaa aag ttt aga gac att ccn aaa tac ata cca gag act gaa gat gat 288 Glu Lys Phe Arg Asp Ile Pro Lys Tyr Ile Pro Glu Thr Glu Asp Asp 85 90 95 tat cca gaa tgc cat tta gca ctg gtt gct aat caa cag gag cct act 336 Tyr Pro Glu Cys His Leu Ala Leu Val Ala Asn Gln Gln Glu Pro Thr 100 105 110 ata cta gaa gtt ggc gac tgc tgc tca tat gga aac ata tta tta agt 384 Ile Leu Glu Val Gly Asp Cys Cys Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggc aac cag aca gct aga atg att aaa tac aac tac cca aca aaa tca 432 Gly Asn Gln Thr Ala Arg Met Ile Lys Tyr Asn Tyr Pro Thr Lys Ser 130 135 140 gga ttc tgt gga ggg gtc ttg tat aaa ata gga tta gtg cta ggt ata 480 Gly Phe Cys Gly Gly Val Leu Tyr Lys Ile Gly Leu Val Leu Gly Ile 145 150 155 160 cat gtt ggg gga aat ggt agg gat ggt ttt tcc gcg atg cta cta aga 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 tca tat ttc act gaa caa cag 549 Ser Tyr Phe Thr Glu Gln Gln 180 <210> 598 <211> 183 <212> PRT <213> Human rhinovirus <400> 598 Gly Pro Gln Glu Glu Phe Gly Arg Ser Leu Ile Lys His Asn Thr Cys 1 5 10 15 Val Val Thr Thr Asp Asn Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 Asn Ile Met Ile Ile Pro Thr His Ala Asp Ala Gly Lys Glu Val Glu 35 40 45 Ile Asp Gly Ile Lys Thr Gln Val Asp Asp Ala Tyr Asp Leu His Asn 50 55 60 Ser Gln Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu His Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Pro Lys Tyr Ile Pro Glu Thr Glu Asp Asp 85 90 95 Tyr Pro Glu Cys His Leu Ala Leu Val Ala Asn Gln Gln Glu Pro Thr 100 105 110 Ile Leu Glu Val Gly Asp Cys Cys Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Asn Gln Thr Ala Arg Met Ile Lys Tyr Asn Tyr Pro Thr Lys Ser 130 135 140 Gly Phe Cys Gly Gly Val Leu Tyr Lys Ile Gly Leu Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 Ser Tyr Phe Thr Glu Gln Gln 180 <210> 599 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_20_Strain_ATCC_VR1130_3C(T19P) <400> 599 ggg cca caa gaa gaa ttt gga cgt agt tta atc aaa cat aac act tgt 48 Gly Pro Gln Glu Glu Phe Gly Arg Ser Leu Ile Lys His Asn Thr Cys 1 5 10 15 gtt gta ccn aca gac aat ggt aag ttc act gga tta ggg atc tat gac 96 Val Val Pro Thr Asp Asn Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 aat ata atg att ata cca aca cat gct gat gct ggg aaa gaa gta gaa 144 Asn Ile Met Ile Ile Pro Thr His Ala Asp Ala Gly Lys Glu Val Glu 35 40 45 ata gat ggg atc aaa act cag gtt gat gat gct tat gat tta cat aat 192 Ile Asp Gly Ile Lys Thr Gln Val Asp Asp Ala Tyr Asp Leu His Asn 50 55 60 tcc cag gga atc aaa ttg gaa att aca gtc ctt aaa ttg cat aga aat 240 Ser Gln Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu His Arg Asn 65 70 75 80 gaa aag ttt aga gac att aga aaa tac ata cca gag act gaa gat gat 288 Glu Lys Phe Arg Asp Ile Arg Lys Tyr Ile Pro Glu Thr Glu Asp Asp 85 90 95 tat cca gaa tgc cat tta gca ctg gtt gct aat caa cag gag cct act 336 Tyr Pro Glu Cys His Leu Ala Leu Val Ala Asn Gln Gln Glu Pro Thr 100 105 110 ata cta gaa gtt ggc gac tgc tgc tca tat gga aac ata tta tta agt 384 Ile Leu Glu Val Gly Asp Cys Cys Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggc aac cag aca gct aga atg att aaa tac aac tac cca aca aaa tca 432 Gly Asn Gln Thr Ala Arg Met Ile Lys Tyr Asn Tyr Pro Thr Lys Ser 130 135 140 gga ttc tgt gga ggg gtc ttg tat aaa ata gga tta gtg cta ggt ata 480 Gly Phe Cys Gly Gly Val Leu Tyr Lys Ile Gly Leu Val Leu Gly Ile 145 150 155 160 cat gtt ggg gga aat ggt agg gat ggt ttt tcc gcg atg cta cta aga 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 tca tat ttc act gaa caa cag 549 Ser Tyr Phe Thr Glu Gln Gln 180 <210> 600 <211> 183 <212> PRT <213> Human rhinovirus <400> 600 Gly Pro Gln Glu Glu Phe Gly Arg Ser Leu Ile Lys His Asn Thr Cys 1 5 10 15 Val Val Pro Thr Asp Asn Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 Asn Ile Met Ile Ile Pro Thr His Ala Asp Ala Gly Lys Glu Val Glu 35 40 45 Ile Asp Gly Ile Lys Thr Gln Val Asp Asp Ala Tyr Asp Leu His Asn 50 55 60 Ser Gln Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu His Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Lys Tyr Ile Pro Glu Thr Glu Asp Asp 85 90 95 Tyr Pro Glu Cys His Leu Ala Leu Val Ala Asn Gln Gln Glu Pro Thr 100 105 110 Ile Leu Glu Val Gly Asp Cys Cys Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Asn Gln Thr Ala Arg Met Ile Lys Tyr Asn Tyr Pro Thr Lys Ser 130 135 140 Gly Phe Cys Gly Gly Val Leu Tyr Lys Ile Gly Leu Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 Ser Tyr Phe Thr Glu Gln Gln 180 <210> 601 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_20_Strain_ATCC_VR1130_3C(T20P) <400> 601 ggg cca caa gaa gaa ttt gga cgt agt tta atc aaa cat aac act tgt 48 Gly Pro Gln Glu Glu Phe Gly Arg Ser Leu Ile Lys His Asn Thr Cys 1 5 10 15 gtt gta aca ccn gac aat ggt aag ttc act gga tta ggg atc tat gac 96 Val Val Thr Pro Asp Asn Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 aat ata atg att ata cca aca cat gct gat gct ggg aaa gaa gta gaa 144 Asn Ile Met Ile Ile Pro Thr His Ala Asp Ala Gly Lys Glu Val Glu 35 40 45 ata gat ggg atc aaa act cag gtt gat gat gct tat gat tta cat aat 192 Ile Asp Gly Ile Lys Thr Gln Val Asp Asp Ala Tyr Asp Leu His Asn 50 55 60 tcc cag gga atc aaa ttg gaa att aca gtc ctt aaa ttg cat aga aat 240 Ser Gln Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu His Arg Asn 65 70 75 80 gaa aag ttt aga gac att aga aaa tac ata cca gag act gaa gat gat 288 Glu Lys Phe Arg Asp Ile Arg Lys Tyr Ile Pro Glu Thr Glu Asp Asp 85 90 95 tat cca gaa tgc cat tta gca ctg gtt gct aat caa cag gag cct act 336 Tyr Pro Glu Cys His Leu Ala Leu Val Ala Asn Gln Gln Glu Pro Thr 100 105 110 ata cta gaa gtt ggc gac tgc tgc tca tat gga aac ata tta tta agt 384 Ile Leu Glu Val Gly Asp Cys Cys Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggc aac cag aca gct aga atg att aaa tac aac tac cca aca aaa tca 432 Gly Asn Gln Thr Ala Arg Met Ile Lys Tyr Asn Tyr Pro Thr Lys Ser 130 135 140 gga ttc tgt gga ggg gtc ttg tat aaa ata gga tta gtg cta ggt ata 480 Gly Phe Cys Gly Gly Val Leu Tyr Lys Ile Gly Leu Val Leu Gly Ile 145 150 155 160 cat gtt ggg gga aat ggt agg gat ggt ttt tcc gcg atg cta cta aga 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 tca tat ttc act gaa caa cag 549 Ser Tyr Phe Thr Glu Gln Gln 180 <210> 602 <211> 183 <212> PRT <213> Human rhinovirus <400> 602 Gly Pro Gln Glu Glu Phe Gly Arg Ser Leu Ile Lys His Asn Thr Cys 1 5 10 15 Val Val Thr Pro Asp Asn Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 Asn Ile Met Ile Ile Pro Thr His Ala Asp Ala Gly Lys Glu Val Glu 35 40 45 Ile Asp Gly Ile Lys Thr Gln Val Asp Asp Ala Tyr Asp Leu His Asn 50 55 60 Ser Gln Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu His Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Lys Tyr Ile Pro Glu Thr Glu Asp Asp 85 90 95 Tyr Pro Glu Cys His Leu Ala Leu Val Ala Asn Gln Gln Glu Pro Thr 100 105 110 Ile Leu Glu Val Gly Asp Cys Cys Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Asn Gln Thr Ala Arg Met Ile Lys Tyr Asn Tyr Pro Thr Lys Ser 130 135 140 Gly Phe Cys Gly Gly Val Leu Tyr Lys Ile Gly Leu Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 Ser Tyr Phe Thr Glu Gln Gln 180 <210> 603 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_20_Strain_ATCC_VR1130_3C(wt) <400> 603 ggg cca caa gaa gaa ttt gga cgt agt tta atc aaa cat aac act tgt 48 Gly Pro Gln Glu Glu Phe Gly Arg Ser Leu Ile Lys His Asn Thr Cys 1 5 10 15 gtt gta aca aca gac aat ggt aag ttc act gga tta ggg atc tat gac 96 Val Val Thr Thr Asp Asn Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 aat ata atg att ata cca aca cat gct gat gct ggg aaa gaa gta gaa 144 Asn Ile Met Ile Ile Pro Thr His Ala Asp Ala Gly Lys Glu Val Glu 35 40 45 ata gat ggg atc aaa act cag gtt gat gat gct tat gat tta cat aat 192 Ile Asp Gly Ile Lys Thr Gln Val Asp Asp Ala Tyr Asp Leu His Asn 50 55 60 tcc cag gga atc aaa ttg gaa att aca gtc ctt aaa ttg cat aga aat 240 Ser Gln Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu His Arg Asn 65 70 75 80 gaa aag ttt aga gac att aga aaa tac ata cca gag act gaa gat gat 288 Glu Lys Phe Arg Asp Ile Arg Lys Tyr Ile Pro Glu Thr Glu Asp Asp 85 90 95 tat cca gaa tgc cat tta gca ctg gtt gct aat caa cag gag cct act 336 Tyr Pro Glu Cys His Leu Ala Leu Val Ala Asn Gln Gln Glu Pro Thr 100 105 110 ata cta gaa gtt ggc gac tgc tgc tca tat gga aac ata tta tta agt 384 Ile Leu Glu Val Gly Asp Cys Cys Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggc aac cag aca gct aga atg att aaa tac aac tac cca aca aaa tca 432 Gly Asn Gln Thr Ala Arg Met Ile Lys Tyr Asn Tyr Pro Thr Lys Ser 130 135 140 gga ttc tgt gga ggg gtc ttg tat aaa ata gga tta gtg cta ggt ata 480 Gly Phe Cys Gly Gly Val Leu Tyr Lys Ile Gly Leu Val Leu Gly Ile 145 150 155 160 cat gtt ggg gga aat ggt agg gat ggt ttt tcc gcg atg cta cta aga 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 tca tat ttc act gaa caa cag 549 Ser Tyr Phe Thr Glu Gln Gln 180 <210> 604 <211> 183 <212> PRT <213> Human rhinovirus <400> 604 Gly Pro Gln Glu Glu Phe Gly Arg Ser Leu Ile Lys His Asn Thr Cys 1 5 10 15 Val Val Thr Thr Asp Asn Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 Asn Ile Met Ile Ile Pro Thr His Ala Asp Ala Gly Lys Glu Val Glu 35 40 45 Ile Asp Gly Ile Lys Thr Gln Val Asp Asp Ala Tyr Asp Leu His Asn 50 55 60 Ser Gln Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu His Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Lys Tyr Ile Pro Glu Thr Glu Asp Asp 85 90 95 Tyr Pro Glu Cys His Leu Ala Leu Val Ala Asn Gln Gln Glu Pro Thr 100 105 110 Ile Leu Glu Val Gly Asp Cys Cys Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Asn Gln Thr Ala Arg Met Ile Lys Tyr Asn Tyr Pro Thr Lys Ser 130 135 140 Gly Phe Cys Gly Gly Val Leu Tyr Lys Ile Gly Leu Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 Ser Tyr Phe Thr Glu Gln Gln 180 <210> 605 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_89_Strain_ATCC_VR1199_3C(I114P) <400> 605 ggc cca gag gaa gag ttt ggt cgc tca cta ctc aaa cat aat tgc tgt 48 Gly Pro Glu Glu Glu Phe Gly Arg Ser Leu Leu Lys His Asn Cys Cys 1 5 10 15 gtt gtg aca acc gac aaa ggc aaa ttc aca ggt ctt ggc ata tat gac 96 Val Val Thr Thr Asp Lys Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 caa gtc atg gta ctt cca aca cat tct gac cca ggc tct gag atc ttg 144 Gln Val Met Val Leu Pro Thr His Ser Asp Pro Gly Ser Glu Ile Leu 35 40 45 gta gat gga gta aaa gtt aag gtc tct gat tcc tat gat ttg cat aac 192 Val Asp Gly Val Lys Val Lys Val Ser Asp Ser Tyr Asp Leu His Asn 50 55 60 cat gag ggt gtt aag cta gag atc aca gtt gtg aaa tta att aga aat 240 His Glu Gly Val Lys Leu Glu Ile Thr Val Val Lys Leu Ile Arg Asn 65 70 75 80 gag aag ttt aaa gac atc aga aaa tat tta ccc tca cgt gaa gat gac 288 Glu Lys Phe Lys Asp Ile Arg Lys Tyr Leu Pro Ser Arg Glu Asp Asp 85 90 95 tat cct gct tgt aac ctt gcc tta cta gct aat caa gat gag cca aca 336 Tyr Pro Ala Cys Asn Leu Ala Leu Leu Ala Asn Gln Asp Glu Pro Thr 100 105 110 ata ccn agt gtt ggt gat gca gta tct tat ggt aac atc tta ttg agt 384 Ile Pro Ser Val Gly Asp Ala Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggt acc aat act gca cga atg atc aag tac cac tac ccg aca aaa gct 432 Gly Thr Asn Thr Ala Arg Met Ile Lys Tyr His Tyr Pro Thr Lys Ala 130 135 140 gga tat tgt ggg ggt gtt ttg tac aag gtt ggc tct att ctt ggt ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Val Gly Ser Ile Leu Gly Ile 145 150 155 160 cat gtt ggt ggc aat ggt aga gat gga ttt tct gca atg ctt ctc aaa 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Lys 165 170 175 tct tat ttt ggt gaa acc cag 549 Ser Tyr Phe Gly Glu Thr Gln 180 <210> 606 <211> 183 <212> PRT <213> Human rhinovirus <400> 606 Gly Pro Glu Glu Glu Phe Gly Arg Ser Leu Leu Lys His Asn Cys Cys 1 5 10 15 Val Val Thr Thr Asp Lys Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 Gln Val Met Val Leu Pro Thr His Ser Asp Pro Gly Ser Glu Ile Leu 35 40 45 Val Asp Gly Val Lys Val Lys Val Ser Asp Ser Tyr Asp Leu His Asn 50 55 60 His Glu Gly Val Lys Leu Glu Ile Thr Val Val Lys Leu Ile Arg Asn 65 70 75 80 Glu Lys Phe Lys Asp Ile Arg Lys Tyr Leu Pro Ser Arg Glu Asp Asp 85 90 95 Tyr Pro Ala Cys Asn Leu Ala Leu Leu Ala Asn Gln Asp Glu Pro Thr 100 105 110 Ile Pro Ser Val Gly Asp Ala Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Thr Asn Thr Ala Arg Met Ile Lys Tyr His Tyr Pro Thr Lys Ala 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Val Gly Ser Ile Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Lys 165 170 175 Ser Tyr Phe Gly Glu Thr Gln 180 <210> 607 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_89_Strain_ATCC_VR1199_3C(K88P) <400> 607 ggc cca gag gaa gag ttt ggt cgc tca cta ctc aaa cat aat tgc tgt 48 Gly Pro Glu Glu Glu Phe Gly Arg Ser Leu Leu Lys His Asn Cys Cys 1 5 10 15 gtt gtg aca acc gac aaa ggc aaa ttc aca ggt ctt ggc ata tat gac 96 Val Val Thr Thr Asp Lys Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 caa gtc atg gta ctt cca aca cat tct gac cca ggc tct gag atc ttg 144 Gln Val Met Val Leu Pro Thr His Ser Asp Pro Gly Ser Glu Ile Leu 35 40 45 gta gat gga gta aaa gtt aag gtc tct gat tcc tat gat ttg cat aac 192 Val Asp Gly Val Lys Val Lys Val Ser Asp Ser Tyr Asp Leu His Asn 50 55 60 cat gag ggt gtt aag cta gag atc aca gtt gtg aaa tta att aga aat 240 His Glu Gly Val Lys Leu Glu Ile Thr Val Val Lys Leu Ile Arg Asn 65 70 75 80 gag aag ttt aaa gac atc aga ccn tat tta ccc tca cgt gaa gat gac 288 Glu Lys Phe Lys Asp Ile Arg Pro Tyr Leu Pro Ser Arg Glu Asp Asp 85 90 95 tat cct gct tgt aac ctt gcc tta cta gct aat caa gat gag cca aca 336 Tyr Pro Ala Cys Asn Leu Ala Leu Leu Ala Asn Gln Asp Glu Pro Thr 100 105 110 ata ata agt gtt ggt gat gca gta tct tat ggt aac atc tta ttg agt 384 Ile Ile Ser Val Gly Asp Ala Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggt acc aat act gca cga atg atc aag tac cac tac ccg aca aaa gct 432 Gly Thr Asn Thr Ala Arg Met Ile Lys Tyr His Tyr Pro Thr Lys Ala 130 135 140 gga tat tgt ggg ggt gtt ttg tac aag gtt ggc tct att ctt ggt ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Val Gly Ser Ile Leu Gly Ile 145 150 155 160 cat gtt ggt ggc aat ggt aga gat gga ttt tct gca atg ctt ctc aaa 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Lys 165 170 175 tct tat ttt ggt gaa acc cag 549 Ser Tyr Phe Gly Glu Thr Gln 180 <210> 608 <211> 183 <212> PRT <213> Human rhinovirus <400> 608 Gly Pro Glu Glu Glu Phe Gly Arg Ser Leu Leu Lys His Asn Cys Cys 1 5 10 15 Val Val Thr Thr Asp Lys Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 Gln Val Met Val Leu Pro Thr His Ser Asp Pro Gly Ser Glu Ile Leu 35 40 45 Val Asp Gly Val Lys Val Lys Val Ser Asp Ser Tyr Asp Leu His Asn 50 55 60 His Glu Gly Val Lys Leu Glu Ile Thr Val Val Lys Leu Ile Arg Asn 65 70 75 80 Glu Lys Phe Lys Asp Ile Arg Pro Tyr Leu Pro Ser Arg Glu Asp Asp 85 90 95 Tyr Pro Ala Cys Asn Leu Ala Leu Leu Ala Asn Gln Asp Glu Pro Thr 100 105 110 Ile Ile Ser Val Gly Asp Ala Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Thr Asn Thr Ala Arg Met Ile Lys Tyr His Tyr Pro Thr Lys Ala 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Val Gly Ser Ile Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Lys 165 170 175 Ser Tyr Phe Gly Glu Thr Gln 180 <210> 609 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_89_Strain_ATCC_VR1199_3C(R87P) <400> 609 ggc cca gag gaa gag ttt ggt cgc tca cta ctc aaa cat aat tgc tgt 48 Gly Pro Glu Glu Glu Phe Gly Arg Ser Leu Leu Lys His Asn Cys Cys 1 5 10 15 gtt gtg aca acc gac aaa ggc aaa ttc aca ggt ctt ggc ata tat gac 96 Val Val Thr Thr Asp Lys Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 caa gtc atg gta ctt cca aca cat tct gac cca ggc tct gag atc ttg 144 Gln Val Met Val Leu Pro Thr His Ser Asp Pro Gly Ser Glu Ile Leu 35 40 45 gta gat gga gta aaa gtt aag gtc tct gat tcc tat gat ttg cat aac 192 Val Asp Gly Val Lys Val Lys Val Ser Asp Ser Tyr Asp Leu His Asn 50 55 60 cat gag ggt gtt aag cta gag atc aca gtt gtg aaa tta att aga aat 240 His Glu Gly Val Lys Leu Glu Ile Thr Val Val Lys Leu Ile Arg Asn 65 70 75 80 gag aag ttt aaa gac atc ccn aaa tat tta ccc tca cgt gaa gat gac 288 Glu Lys Phe Lys Asp Ile Pro Lys Tyr Leu Pro Ser Arg Glu Asp Asp 85 90 95 tat cct gct tgt aac ctt gcc tta cta gct aat caa gat gag cca aca 336 Tyr Pro Ala Cys Asn Leu Ala Leu Leu Ala Asn Gln Asp Glu Pro Thr 100 105 110 ata ata agt gtt ggt gat gca gta tct tat ggt aac atc tta ttg agt 384 Ile Ile Ser Val Gly Asp Ala Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggt acc aat act gca cga atg atc aag tac cac tac ccg aca aaa gct 432 Gly Thr Asn Thr Ala Arg Met Ile Lys Tyr His Tyr Pro Thr Lys Ala 130 135 140 gga tat tgt ggg ggt gtt ttg tac aag gtt ggc tct att ctt ggt ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Val Gly Ser Ile Leu Gly Ile 145 150 155 160 cat gtt ggt ggc aat ggt aga gat gga ttt tct gca atg ctt ctc aaa 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Lys 165 170 175 tct tat ttt ggt gaa acc cag 549 Ser Tyr Phe Gly Glu Thr Gln 180 <210> 610 <211> 183 <212> PRT <213> Human rhinovirus <400> 610 Gly Pro Glu Glu Glu Phe Gly Arg Ser Leu Leu Lys His Asn Cys Cys 1 5 10 15 Val Val Thr Thr Asp Lys Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 Gln Val Met Val Leu Pro Thr His Ser Asp Pro Gly Ser Glu Ile Leu 35 40 45 Val Asp Gly Val Lys Val Lys Val Ser Asp Ser Tyr Asp Leu His Asn 50 55 60 His Glu Gly Val Lys Leu Glu Ile Thr Val Val Lys Leu Ile Arg Asn 65 70 75 80 Glu Lys Phe Lys Asp Ile Pro Lys Tyr Leu Pro Ser Arg Glu Asp Asp 85 90 95 Tyr Pro Ala Cys Asn Leu Ala Leu Leu Ala Asn Gln Asp Glu Pro Thr 100 105 110 Ile Ile Ser Val Gly Asp Ala Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Thr Asn Thr Ala Arg Met Ile Lys Tyr His Tyr Pro Thr Lys Ala 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Val Gly Ser Ile Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Lys 165 170 175 Ser Tyr Phe Gly Glu Thr Gln 180 <210> 611 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_89_Strain_ATCC_VR1199_3C(T112P) <400> 611 ggc cca gag gaa gag ttt ggt cgc tca cta ctc aaa cat aat tgc tgt 48 Gly Pro Glu Glu Glu Phe Gly Arg Ser Leu Leu Lys His Asn Cys Cys 1 5 10 15 gtt gtg aca acc gac aaa ggc aaa ttc aca ggt ctt ggc ata tat gac 96 Val Val Thr Thr Asp Lys Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 caa gtc atg gta ctt cca aca cat tct gac cca ggc tct gag atc ttg 144 Gln Val Met Val Leu Pro Thr His Ser Asp Pro Gly Ser Glu Ile Leu 35 40 45 gta gat gga gta aaa gtt aag gtc tct gat tcc tat gat ttg cat aac 192 Val Asp Gly Val Lys Val Lys Val Ser Asp Ser Tyr Asp Leu His Asn 50 55 60 cat gag ggt gtt aag cta gag atc aca gtt gtg aaa tta att aga aat 240 His Glu Gly Val Lys Leu Glu Ile Thr Val Val Lys Leu Ile Arg Asn 65 70 75 80 gag aag ttt aaa gac atc aga aaa tat tta ccc tca cgt gaa gat gac 288 Glu Lys Phe Lys Asp Ile Arg Lys Tyr Leu Pro Ser Arg Glu Asp Asp 85 90 95 tat cct gct tgt aac ctt gcc tta cta gct aat caa gat gag cca ccn 336 Tyr Pro Ala Cys Asn Leu Ala Leu Leu Ala Asn Gln Asp Glu Pro Pro 100 105 110 ata ata agt gtt ggt gat gca gta tct tat ggt aac atc tta ttg agt 384 Ile Ile Ser Val Gly Asp Ala Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggt acc aat act gca cga atg atc aag tac cac tac ccg aca aaa gct 432 Gly Thr Asn Thr Ala Arg Met Ile Lys Tyr His Tyr Pro Thr Lys Ala 130 135 140 gga tat tgt ggg ggt gtt ttg tac aag gtt ggc tct att ctt ggt ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Val Gly Ser Ile Leu Gly Ile 145 150 155 160 cat gtt ggt ggc aat ggt aga gat gga ttt tct gca atg ctt ctc aaa 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Lys 165 170 175 tct tat ttt ggt gaa acc cag 549 Ser Tyr Phe Gly Glu Thr Gln 180 <210> 612 <211> 183 <212> PRT <213> Human rhinovirus <400> 612 Gly Pro Glu Glu Glu Phe Gly Arg Ser Leu Leu Lys His Asn Cys Cys 1 5 10 15 Val Val Thr Thr Asp Lys Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 Gln Val Met Val Leu Pro Thr His Ser Asp Pro Gly Ser Glu Ile Leu 35 40 45 Val Asp Gly Val Lys Val Lys Val Ser Asp Ser Tyr Asp Leu His Asn 50 55 60 His Glu Gly Val Lys Leu Glu Ile Thr Val Val Lys Leu Ile Arg Asn 65 70 75 80 Glu Lys Phe Lys Asp Ile Arg Lys Tyr Leu Pro Ser Arg Glu Asp Asp 85 90 95 Tyr Pro Ala Cys Asn Leu Ala Leu Leu Ala Asn Gln Asp Glu Pro Pro 100 105 110 Ile Ile Ser Val Gly Asp Ala Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Thr Asn Thr Ala Arg Met Ile Lys Tyr His Tyr Pro Thr Lys Ala 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Val Gly Ser Ile Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Lys 165 170 175 Ser Tyr Phe Gly Glu Thr Gln 180 <210> 613 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_89_Strain_ATCC_VR1199_3C(T19P) <400> 613 ggc cca gag gaa gag ttt ggt cgc tca cta ctc aaa cat aat tgc tgt 48 Gly Pro Glu Glu Glu Phe Gly Arg Ser Leu Leu Lys His Asn Cys Cys 1 5 10 15 gtt gtg ccn acc gac aaa ggc aaa ttc aca ggt ctt ggc ata tat gac 96 Val Val Pro Thr Asp Lys Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 caa gtc atg gta ctt cca aca cat tct gac cca ggc tct gag atc ttg 144 Gln Val Met Val Leu Pro Thr His Ser Asp Pro Gly Ser Glu Ile Leu 35 40 45 gta gat gga gta aaa gtt aag gtc tct gat tcc tat gat ttg cat aac 192 Val Asp Gly Val Lys Val Lys Val Ser Asp Ser Tyr Asp Leu His Asn 50 55 60 cat gag ggt gtt aag cta gag atc aca gtt gtg aaa tta att aga aat 240 His Glu Gly Val Lys Leu Glu Ile Thr Val Val Lys Leu Ile Arg Asn 65 70 75 80 gag aag ttt aaa gac atc aga aaa tat tta ccc tca cgt gaa gat gac 288 Glu Lys Phe Lys Asp Ile Arg Lys Tyr Leu Pro Ser Arg Glu Asp Asp 85 90 95 tat cct gct tgt aac ctt gcc tta cta gct aat caa gat gag cca aca 336 Tyr Pro Ala Cys Asn Leu Ala Leu Leu Ala Asn Gln Asp Glu Pro Thr 100 105 110 ata ata agt gtt ggt gat gca gta tct tat ggt aac atc tta ttg agt 384 Ile Ile Ser Val Gly Asp Ala Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggt acc aat act gca cga atg atc aag tac cac tac ccg aca aaa gct 432 Gly Thr Asn Thr Ala Arg Met Ile Lys Tyr His Tyr Pro Thr Lys Ala 130 135 140 gga tat tgt ggg ggt gtt ttg tac aag gtt ggc tct att ctt ggt ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Val Gly Ser Ile Leu Gly Ile 145 150 155 160 cat gtt ggt ggc aat ggt aga gat gga ttt tct gca atg ctt ctc aaa 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Lys 165 170 175 tct tat ttt ggt gaa acc cag 549 Ser Tyr Phe Gly Glu Thr Gln 180 <210> 614 <211> 183 <212> PRT <213> Human rhinovirus <400> 614 Gly Pro Glu Glu Glu Phe Gly Arg Ser Leu Leu Lys His Asn Cys Cys 1 5 10 15 Val Val Pro Thr Asp Lys Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 Gln Val Met Val Leu Pro Thr His Ser Asp Pro Gly Ser Glu Ile Leu 35 40 45 Val Asp Gly Val Lys Val Lys Val Ser Asp Ser Tyr Asp Leu His Asn 50 55 60 His Glu Gly Val Lys Leu Glu Ile Thr Val Val Lys Leu Ile Arg Asn 65 70 75 80 Glu Lys Phe Lys Asp Ile Arg Lys Tyr Leu Pro Ser Arg Glu Asp Asp 85 90 95 Tyr Pro Ala Cys Asn Leu Ala Leu Leu Ala Asn Gln Asp Glu Pro Thr 100 105 110 Ile Ile Ser Val Gly Asp Ala Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Thr Asn Thr Ala Arg Met Ile Lys Tyr His Tyr Pro Thr Lys Ala 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Val Gly Ser Ile Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Lys 165 170 175 Ser Tyr Phe Gly Glu Thr Gln 180 <210> 615 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_89_Strain_ATCC_VR1199_3C(T20P) <400> 615 ggc cca gag gaa gag ttt ggt cgc tca cta ctc aaa cat aat tgc tgt 48 Gly Pro Glu Glu Glu Phe Gly Arg Ser Leu Leu Lys His Asn Cys Cys 1 5 10 15 gtt gtg aca ccn gac aaa ggc aaa ttc aca ggt ctt ggc ata tat gac 96 Val Val Thr Pro Asp Lys Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 caa gtc atg gta ctt cca aca cat tct gac cca ggc tct gag atc ttg 144 Gln Val Met Val Leu Pro Thr His Ser Asp Pro Gly Ser Glu Ile Leu 35 40 45 gta gat gga gta aaa gtt aag gtc tct gat tcc tat gat ttg cat aac 192 Val Asp Gly Val Lys Val Lys Val Ser Asp Ser Tyr Asp Leu His Asn 50 55 60 cat gag ggt gtt aag cta gag atc aca gtt gtg aaa tta att aga aat 240 His Glu Gly Val Lys Leu Glu Ile Thr Val Val Lys Leu Ile Arg Asn 65 70 75 80 gag aag ttt aaa gac atc aga aaa tat tta ccc tca cgt gaa gat gac 288 Glu Lys Phe Lys Asp Ile Arg Lys Tyr Leu Pro Ser Arg Glu Asp Asp 85 90 95 tat cct gct tgt aac ctt gcc tta cta gct aat caa gat gag cca aca 336 Tyr Pro Ala Cys Asn Leu Ala Leu Leu Ala Asn Gln Asp Glu Pro Thr 100 105 110 ata ata agt gtt ggt gat gca gta tct tat ggt aac atc tta ttg agt 384 Ile Ile Ser Val Gly Asp Ala Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggt acc aat act gca cga atg atc aag tac cac tac ccg aca aaa gct 432 Gly Thr Asn Thr Ala Arg Met Ile Lys Tyr His Tyr Pro Thr Lys Ala 130 135 140 gga tat tgt ggg ggt gtt ttg tac aag gtt ggc tct att ctt ggt ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Val Gly Ser Ile Leu Gly Ile 145 150 155 160 cat gtt ggt ggc aat ggt aga gat gga ttt tct gca atg ctt ctc aaa 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Lys 165 170 175 tct tat ttt ggt gaa acc cag 549 Ser Tyr Phe Gly Glu Thr Gln 180 <210> 616 <211> 183 <212> PRT <213> Human rhinovirus <400> 616 Gly Pro Glu Glu Glu Phe Gly Arg Ser Leu Leu Lys His Asn Cys Cys 1 5 10 15 Val Val Thr Pro Asp Lys Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 Gln Val Met Val Leu Pro Thr His Ser Asp Pro Gly Ser Glu Ile Leu 35 40 45 Val Asp Gly Val Lys Val Lys Val Ser Asp Ser Tyr Asp Leu His Asn 50 55 60 His Glu Gly Val Lys Leu Glu Ile Thr Val Val Lys Leu Ile Arg Asn 65 70 75 80 Glu Lys Phe Lys Asp Ile Arg Lys Tyr Leu Pro Ser Arg Glu Asp Asp 85 90 95 Tyr Pro Ala Cys Asn Leu Ala Leu Leu Ala Asn Gln Asp Glu Pro Thr 100 105 110 Ile Ile Ser Val Gly Asp Ala Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Thr Asn Thr Ala Arg Met Ile Lys Tyr His Tyr Pro Thr Lys Ala 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Val Gly Ser Ile Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Lys 165 170 175 Ser Tyr Phe Gly Glu Thr Gln 180 <210> 617 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_89_Strain_ATCC_VR1199_3C(wt)) <400> 617 ggc cca gag gaa gag ttt ggt cgc tca cta ctc aaa cat aat tgc tgt 48 Gly Pro Glu Glu Glu Phe Gly Arg Ser Leu Leu Lys His Asn Cys Cys 1 5 10 15 gtt gtg aca acc gac aaa ggc aaa ttc aca ggt ctt ggc ata tat gac 96 Val Val Thr Thr Asp Lys Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 caa gtc atg gta ctt cca aca cat tct gac cca ggc tct gag atc ttg 144 Gln Val Met Val Leu Pro Thr His Ser Asp Pro Gly Ser Glu Ile Leu 35 40 45 gta gat gga gta aaa gtt aag gtc tct gat tcc tat gat ttg cat aac 192 Val Asp Gly Val Lys Val Lys Val Ser Asp Ser Tyr Asp Leu His Asn 50 55 60 cat gag ggt gtt aag cta gag atc aca gtt gtg aaa tta att aga aat 240 His Glu Gly Val Lys Leu Glu Ile Thr Val Val Lys Leu Ile Arg Asn 65 70 75 80 gag aag ttt aaa gac atc aga aaa tat tta ccc tca cgt gaa gat gac 288 Glu Lys Phe Lys Asp Ile Arg Lys Tyr Leu Pro Ser Arg Glu Asp Asp 85 90 95 tat cct gct tgt aac ctt gcc tta cta gct aat caa gat gag cca aca 336 Tyr Pro Ala Cys Asn Leu Ala Leu Leu Ala Asn Gln Asp Glu Pro Thr 100 105 110 ata ata agt gtt ggt gat gca gta tct tat ggt aac atc tta ttg agt 384 Ile Ile Ser Val Gly Asp Ala Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggt acc aat act gca cga atg atc aag tac cac tac ccg aca aaa gct 432 Gly Thr Asn Thr Ala Arg Met Ile Lys Tyr His Tyr Pro Thr Lys Ala 130 135 140 gga tat tgt ggg ggt gtt ttg tac aag gtt ggc tct att ctt ggt ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Val Gly Ser Ile Leu Gly Ile 145 150 155 160 cat gtt ggt ggc aat ggt aga gat gga ttt tct gca atg ctt ctc aaa 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Lys 165 170 175 tct tat ttt ggt gaa acc cag 549 Ser Tyr Phe Gly Glu Thr Gln 180 <210> 618 <211> 183 <212> PRT <213> Human rhinovirus <400> 618 Gly Pro Glu Glu Glu Phe Gly Arg Ser Leu Leu Lys His Asn Cys Cys 1 5 10 15 Val Val Thr Thr Asp Lys Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 20 25 30 Gln Val Met Val Leu Pro Thr His Ser Asp Pro Gly Ser Glu Ile Leu 35 40 45 Val Asp Gly Val Lys Val Lys Val Ser Asp Ser Tyr Asp Leu His Asn 50 55 60 His Glu Gly Val Lys Leu Glu Ile Thr Val Val Lys Leu Ile Arg Asn 65 70 75 80 Glu Lys Phe Lys Asp Ile Arg Lys Tyr Leu Pro Ser Arg Glu Asp Asp 85 90 95 Tyr Pro Ala Cys Asn Leu Ala Leu Leu Ala Asn Gln Asp Glu Pro Thr 100 105 110 Ile Ile Ser Val Gly Asp Ala Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Thr Asn Thr Ala Arg Met Ile Lys Tyr His Tyr Pro Thr Lys Ala 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Val Gly Ser Ile Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Lys 165 170 175 Ser Tyr Phe Gly Glu Thr Gln 180 <210> 619 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_A2_3C_X02316 <400> 619 gga cca gag gag gaa ttt ggg atg tct tta att aaa cat aac tca tgt 48 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 gtt att aca aca gaa aat ggg aaa ttc aca ggt ctt gga gta tac gac 96 Val Ile Thr Thr Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 aga ttt gtg gtc gta cca aca cat gca gat cct gga aag gaa att cag 144 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 gtt gat ggt ata act aca aaa gtc att gac tca tat gac cta tac aac 192 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 aag aat ggg ata aag cta gaa ata aca gta ctt aaa tta gat aga aat 240 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 gaa aaa ttt aga gat atc agg aga tat ata cct aac aat gaa gat gat 288 Glu Lys Phe Arg Asp Ile Arg Arg Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 tac ccc aat tgc aac tta gca ctg cta gca aac cag cct gaa cca act 336 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Gln Pro Glu Pro Thr 100 105 110 ata atc aat gtt gga gat gtt gta tcc tat ggc aat ata ctg ctc agt 384 Ile Ile Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggc aac caa acg gct aga atg ctt aaa tac agt tac cca act aaa tct 432 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 ggt tac tgt gga ggt gtc tta tac aaa att ggg caa gtg ctt gga ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 cat gtt ggg ggc aat ggt agg gat ggt ttc tca gct atg tta ctc aga 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 tcc tat ttc act gat gtt cag 549 Ser Tyr Phe Thr Asp Val Gln 180 <210> 620 <211> 183 <212> PRT <213> Human rhinovirus <400> 620 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 Val Ile Thr Thr Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Arg Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Gln Pro Glu Pro Thr 100 105 110 Ile Ile Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 Ser Tyr Phe Thr Asp Val Gln 180 <210> 621 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_A2_3C_X02316_3C(I114P) <400> 621 gga cca gag gag gaa ttt ggg atg tct tta att aaa cat aac tca tgt 48 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 gtt att aca aca gaa aat ggg aaa ttc aca ggt ctt gga gta tac gac 96 Val Ile Thr Thr Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 aga ttt gtg gtc gta cca aca cat gca gat cct gga aag gaa att cag 144 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 gtt gat ggt ata act aca aaa gtc att gac tca tat gac cta tac aac 192 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 aag aat ggg ata aag cta gaa ata aca gta ctt aaa tta gat aga aat 240 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 gaa aaa ttt aga gat atc agg aga tat ata cct aac aat gaa gat gat 288 Glu Lys Phe Arg Asp Ile Arg Arg Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 tac ccc aat tgc aac tta gca ctg cta gca aac cag cct gaa cca act 336 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Gln Pro Glu Pro Thr 100 105 110 ata ccn aat gtt gga gat gtt gta tcc tat ggc aat ata ctg ctc agt 384 Ile Pro Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggc aac caa acg gct aga atg ctt aaa tac agt tac cca act aaa tct 432 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 ggt tac tgt gga ggt gtc tta tac aaa att ggg caa gtg ctt gga ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 cat gtt ggg ggc aat ggt agg gat ggt ttc tca gct atg tta ctc aga 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 tcc tat ttc act gat gtt cag 549 Ser Tyr Phe Thr Asp Val Gln 180 <210> 622 <211> 183 <212> PRT <213> Human rhinovirus <400> 622 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 Val Ile Thr Thr Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Arg Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Gln Pro Glu Pro Thr 100 105 110 Ile Pro Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 Ser Tyr Phe Thr Asp Val Gln 180 <210> 623 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_A2_3C_X02316_3C(Q108P) <400> 623 gga cca gag gag gaa ttt ggg atg tct tta att aaa cat aac tca tgt 48 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 gtt att aca aca gaa aat ggg aaa ttc aca ggt ctt gga gta tac gac 96 Val Ile Thr Thr Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 aga ttt gtg gtc gta cca aca cat gca gat cct gga aag gaa att cag 144 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 gtt gat ggt ata act aca aaa gtc att gac tca tat gac cta tac aac 192 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 aag aat ggg ata aag cta gaa ata aca gta ctt aaa tta gat aga aat 240 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 gaa aaa ttt aga gat atc agg aga tat ata cct aac aat gaa gat gat 288 Glu Lys Phe Arg Asp Ile Arg Arg Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 tac ccc aat tgc aac tta gca ctg cta gca aac ccn cct gaa cca act 336 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Pro Pro Glu Pro Thr 100 105 110 ata atc aat gtt gga gat gtt gta tcc tat ggc aat ata ctg ctc agt 384 Ile Ile Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggc aac caa acg gct aga atg ctt aaa tac agt tac cca act aaa tct 432 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 ggt tac tgt gga ggt gtc tta tac aaa att ggg caa gtg ctt gga ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 cat gtt ggg ggc aat ggt agg gat ggt ttc tca gct atg tta ctc aga 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 tcc tat ttc act gat gtt cag 549 Ser Tyr Phe Thr Asp Val Gln 180 <210> 624 <211> 183 <212> PRT <213> Human rhinovirus <400> 624 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 Val Ile Thr Thr Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Arg Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Pro Pro Glu Pro Thr 100 105 110 Ile Ile Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 Ser Tyr Phe Thr Asp Val Gln 180 <210> 625 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_A2_3C_X02316_3C(R87P) <400> 625 gga cca gag gag gaa ttt ggg atg tct tta att aaa cat aac tca tgt 48 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 gtt att aca aca gaa aat ggg aaa ttc aca ggt ctt gga gta tac gac 96 Val Ile Thr Thr Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 aga ttt gtg gtc gta cca aca cat gca gat cct gga aag gaa att cag 144 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 gtt gat ggt ata act aca aaa gtc att gac tca tat gac cta tac aac 192 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 aag aat ggg ata aag cta gaa ata aca gta ctt aaa tta gat aga aat 240 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 gaa aaa ttt aga gat atc ccn aga tat ata cct aac aat gaa gat gat 288 Glu Lys Phe Arg Asp Ile Pro Arg Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 tac ccc aat tgc aac tta gca ctg cta gca aac cag cct gaa cca act 336 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Gln Pro Glu Pro Thr 100 105 110 ata atc aat gtt gga gat gtt gta tcc tat ggc aat ata ctg ctc agt 384 Ile Ile Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggc aac caa acg gct aga atg ctt aaa tac agt tac cca act aaa tct 432 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 ggt tac tgt gga ggt gtc tta tac aaa att ggg caa gtg ctt gga ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 cat gtt ggg ggc aat ggt agg gat ggt ttc tca gct atg tta ctc aga 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 tcc tat ttc act gat gtt cag 549 Ser Tyr Phe Thr Asp Val Gln 180 <210> 626 <211> 183 <212> PRT <213> Human rhinovirus <400> 626 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 Val Ile Thr Thr Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Pro Arg Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Gln Pro Glu Pro Thr 100 105 110 Ile Ile Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 Ser Tyr Phe Thr Asp Val Gln 180 <210> 627 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_A2_3C_X02316_3C(R88P) <400> 627 gga cca gag gag gaa ttt ggg atg tct tta att aaa cat aac tca tgt 48 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 gtt att aca aca gaa aat ggg aaa ttc aca ggt ctt gga gta tac gac 96 Val Ile Thr Thr Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 aga ttt gtg gtc gta cca aca cat gca gat cct gga aag gaa att cag 144 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 gtt gat ggt ata act aca aaa gtc att gac tca tat gac cta tac aac 192 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 aag aat ggg ata aag cta gaa ata aca gta ctt aaa tta gat aga aat 240 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 gaa aaa ttt aga gat atc agg ccn tat ata cct aac aat gaa gat gat 288 Glu Lys Phe Arg Asp Ile Arg Pro Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 tac ccc aat tgc aac tta gca ctg cta gca aac cag cct gaa cca act 336 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Gln Pro Glu Pro Thr 100 105 110 ata atc aat gtt gga gat gtt gta tcc tat ggc aat ata ctg ctc agt 384 Ile Ile Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggc aac caa acg gct aga atg ctt aaa tac agt tac cca act aaa tct 432 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 ggt tac tgt gga ggt gtc tta tac aaa att ggg caa gtg ctt gga ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 cat gtt ggg ggc aat ggt agg gat ggt ttc tca gct atg tta ctc aga 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 tcc tat ttc act gat gtt cag 549 Ser Tyr Phe Thr Asp Val Gln 180 <210> 628 <211> 183 <212> PRT <213> Human rhinovirus <400> 628 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 Val Ile Thr Thr Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Pro Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Gln Pro Glu Pro Thr 100 105 110 Ile Ile Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 Ser Tyr Phe Thr Asp Val Gln 180 <210> 629 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_A2_3C_X02316_3C(T112P) <400> 629 gga cca gag gag gaa ttt ggg atg tct tta att aaa cat aac tca tgt 48 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 gtt att aca aca gaa aat ggg aaa ttc aca ggt ctt gga gta tac gac 96 Val Ile Thr Thr Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 aga ttt gtg gtc gta cca aca cat gca gat cct gga aag gaa att cag 144 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 gtt gat ggt ata act aca aaa gtc att gac tca tat gac cta tac aac 192 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 aag aat ggg ata aag cta gaa ata aca gta ctt aaa tta gat aga aat 240 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 gaa aaa ttt aga gat atc agg aga tat ata cct aac aat gaa gat gat 288 Glu Lys Phe Arg Asp Ile Arg Arg Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 tac ccc aat tgc aac tta gca ctg cta gca aac cag cct gaa cca ccn 336 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Gln Pro Glu Pro Pro 100 105 110 ata atc aat gtt gga gat gtt gta tcc tat ggc aat ata ctg ctc agt 384 Ile Ile Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggc aac caa acg gct aga atg ctt aaa tac agt tac cca act aaa tct 432 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 ggt tac tgt gga ggt gtc tta tac aaa att ggg caa gtg ctt gga ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 cat gtt ggg ggc aat ggt agg gat ggt ttc tca gct atg tta ctc aga 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 tcc tat ttc act gat gtt cag 549 Ser Tyr Phe Thr Asp Val Gln 180 <210> 630 <211> 183 <212> PRT <213> Human rhinovirus <400> 630 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 Val Ile Thr Thr Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Arg Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Gln Pro Glu Pro Pro 100 105 110 Ile Ile Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 Ser Tyr Phe Thr Asp Val Gln 180 <210> 631 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_A2_3C_X02316_3C(T19P) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <400> 631 gga cca gag gag gaa ttt ggg atg tct tta att aaa cat aac tca tgt 48 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 gtt att ccn aca gaa aat ggg aaa ttc aca ggt ctt gga gta tac gac 96 Val Ile Pro Thr Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 aga ttt gtg gtc gta cca aca cat gca gat cct gga aag gaa att cag 144 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 gtt gat ggt ata act aca aaa gtc att gac tca tat gac cta tac aac 192 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 aag aat ggg ata aag cta gaa ata aca gta ctt aaa tta gat aga aat 240 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 gaa aaa ttt aga gat atc agg aga tat ata cct aac aat gaa gat gat 288 Glu Lys Phe Arg Asp Ile Arg Arg Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 tac ccc aat tgc aac tta gca ctg cta gca aac cag cct gaa cca act 336 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Gln Pro Glu Pro Thr 100 105 110 ata atc aat gtt gga gat gtt gta tcc tat ggc aat ata ctg ctc agt 384 Ile Ile Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggc aac caa acg gct aga atg ctt aaa tac agt tac cca act aaa tct 432 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 ggt tac tgt gga ggt gtc tta tac aaa att ggg caa gtg ctt gga ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 cat gtt ggg ggc aat ggt agg gat ggt ttc tca gct atg tta ctc aga 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 tcc tat ttc act gat gtt cag 549 Ser Tyr Phe Thr Asp Val Gln 180 <210> 632 <211> 183 <212> PRT <213> Human rhinovirus <400> 632 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 Val Ile Pro Thr Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Arg Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Gln Pro Glu Pro Thr 100 105 110 Ile Ile Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 Ser Tyr Phe Thr Asp Val Gln 180 <210> 633 <211> 549 <212> DNA <213> Human rhinovirus <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> n is c, g, a, t/u <220> <221> misc_feature <222> (1)..(549) <223> Human_Rhinovirus_A2_3C_X02316_3C(T20P) <400> 633 gga cca gag gag gaa ttt ggg atg tct tta att aaa cat aac tca tgt 48 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 gtt att aca ccn gaa aat ggg aaa ttc aca ggt ctt gga gta tac gac 96 Val Ile Thr Pro Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 aga ttt gtg gtc gta cca aca cat gca gat cct gga aag gaa att cag 144 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 gtt gat ggt ata act aca aaa gtc att gac tca tat gac cta tac aac 192 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 aag aat ggg ata aag cta gaa ata aca gta ctt aaa tta gat aga aat 240 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 gaa aaa ttt aga gat atc agg aga tat ata cct aac aat gaa gat gat 288 Glu Lys Phe Arg Asp Ile Arg Arg Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 tac ccc aat tgc aac tta gca ctg cta gca aac cag cct gaa cca act 336 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Gln Pro Glu Pro Thr 100 105 110 ata atc aat gtt gga gat gtt gta tcc tat ggc aat ata ctg ctc agt 384 Ile Ile Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 ggc aac caa acg gct aga atg ctt aaa tac agt tac cca act aaa tct 432 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 ggt tac tgt gga ggt gtc tta tac aaa att ggg caa gtg ctt gga ata 480 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 cat gtt ggg ggc aat ggt agg gat ggt ttc tca gct atg tta ctc aga 528 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 tcc tat ttc act gat gtt cag 549 Ser Tyr Phe Thr Asp Val Gln 180 <210> 634 <211> 183 <212> PRT <213> Human rhinovirus <400> 634 Gly Pro Glu Glu Glu Phe Gly Met Ser Leu Ile Lys His Asn Ser Cys 1 5 10 15 Val Ile Thr Pro Glu Asn Gly Lys Phe Thr Gly Leu Gly Val Tyr Asp 20 25 30 Arg Phe Val Val Val Pro Thr His Ala Asp Pro Gly Lys Glu Ile Gln 35 40 45 Val Asp Gly Ile Thr Thr Lys Val Ile Asp Ser Tyr Asp Leu Tyr Asn 50 55 60 Lys Asn Gly Ile Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Arg Tyr Ile Pro Asn Asn Glu Asp Asp 85 90 95 Tyr Pro Asn Cys Asn Leu Ala Leu Leu Ala Asn Gln Pro Glu Pro Thr 100 105 110 Ile Ile Asn Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 115 120 125 Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Ser Tyr Pro Thr Lys Ser 130 135 140 Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu Arg 165 170 175 Ser Tyr Phe Thr Asp Val Gln 180 <210> 635 <211> 615 <212> DNA <213> Porcine Teschovirus <220> <221> CDS <222> (1)..(615) <220> <221> misc_feature <222> (1)..(615) <223> Porcine_Teschovirus_3C(wt)_NC003985 <400> 635 ggg cca aaa gga caa gcc aat atg gaa atg gaa aga act tta atg aag 48 Gly Pro Lys Gly Gln Ala Asn Met Glu Met Glu Arg Thr Leu Met Lys 1 5 10 15 aag aac ata gtg gaa atg aca tat gag aag cac aac ggg agg ttt caa 96 Lys Asn Ile Val Glu Met Thr Tyr Glu Lys His Asn Gly Arg Phe Gln 20 25 30 act acc aca gtc ctg ttt gtg cgt gat cgg att ttt ctg atc aac act 144 Thr Thr Thr Val Leu Phe Val Arg Asp Arg Ile Phe Leu Ile Asn Thr 35 40 45 cac att ttg tca tca att aag aac ttc cac tat gag aac act gag att 192 His Ile Leu Ser Ser Ile Lys Asn Phe His Tyr Glu Asn Thr Glu Ile 50 55 60 cca gca gct tca gtt cag aaa gtt cag gcc ata ttt gat gga cat cca 240 Pro Ala Ala Ser Val Gln Lys Val Gln Ala Ile Phe Asp Gly His Pro 65 70 75 80 tct gat gta act gca gtc cag ttc act gtt gga cga caa tac cgt gac 288 Ser Asp Val Thr Ala Val Gln Phe Thr Val Gly Arg Gln Tyr Arg Asp 85 90 95 atc aca agc aat ttt ata atc agt ctg cca aat cct ggc aca cca ata 336 Ile Thr Ser Asn Phe Ile Ile Ser Leu Pro Asn Pro Gly Thr Pro Ile 100 105 110 gtt ggc ttg atg aag aca gaa ggt agc agt tat atc tgg tct ggt gaa 384 Val Gly Leu Met Lys Thr Glu Gly Ser Ser Tyr Ile Trp Ser Gly Glu 115 120 125 tgt ctc cca ttt aaa aat aca atg aac act tat gaa ggc tgt gtt cct 432 Cys Leu Pro Phe Lys Asn Thr Met Asn Thr Tyr Glu Gly Cys Val Pro 130 135 140 cat gtg tta cca tat aag gcc gtg aca gcc cat ggc tac tgt ggc tct 480 His Val Leu Pro Tyr Lys Ala Val Thr Ala His Gly Tyr Cys Gly Ser 145 150 155 160 gtc atg gtc gca gat gct gga gtc tgg aaa ggc att tgt ggc ata cac 528 Val Met Val Ala Asp Ala Gly Val Trp Lys Gly Ile Cys Gly Ile His 165 170 175 tcc tta gga gat ggt gcc ata ggt gca gca aca gtt ttg tca aga cag 576 Ser Leu Gly Asp Gly Ala Ile Gly Ala Ala Thr Val Leu Ser Arg Gln 180 185 190 cat ttg ctc aac ctt ctt gag gga ttc ctg gaa ttc cag 615 His Leu Leu Asn Leu Leu Glu Gly Phe Leu Glu Phe Gln 195 200 205 <210> 636 <211> 205 <212> PRT <213> Porcine Teschovirus <400> 636 Gly Pro Lys Gly Gln Ala Asn Met Glu Met Glu Arg Thr Leu Met Lys 1 5 10 15 Lys Asn Ile Val Glu Met Thr Tyr Glu Lys His Asn Gly Arg Phe Gln 20 25 30 Thr Thr Thr Val Leu Phe Val Arg Asp Arg Ile Phe Leu Ile Asn Thr 35 40 45 His Ile Leu Ser Ser Ile Lys Asn Phe His Tyr Glu Asn Thr Glu Ile 50 55 60 Pro Ala Ala Ser Val Gln Lys Val Gln Ala Ile Phe Asp Gly His Pro 65 70 75 80 Ser Asp Val Thr Ala Val Gln Phe Thr Val Gly Arg Gln Tyr Arg Asp 85 90 95 Ile Thr Ser Asn Phe Ile Ile Ser Leu Pro Asn Pro Gly Thr Pro Ile 100 105 110 Val Gly Leu Met Lys Thr Glu Gly Ser Ser Tyr Ile Trp Ser Gly Glu 115 120 125 Cys Leu Pro Phe Lys Asn Thr Met Asn Thr Tyr Glu Gly Cys Val Pro 130 135 140 His Val Leu Pro Tyr Lys Ala Val Thr Ala His Gly Tyr Cys Gly Ser 145 150 155 160 Val Met Val Ala Asp Ala Gly Val Trp Lys Gly Ile Cys Gly Ile His 165 170 175 Ser Leu Gly Asp Gly Ala Ile Gly Ala Ala Thr Val Leu Ser Arg Gln 180 185 190 His Leu Leu Asn Leu Leu Glu Gly Phe Leu Glu Phe Gln 195 200 205 <210> 637 <211> 525 <212> DNA <213> Seneca Valley Virus <220> <221> CDS <222> (1)..(525) <220> <221> misc_feature <222> (1)..(525) <223> Seneca_Valley_Virus_3C(wt) <400> 637 gac atg ggc ttt gag gct gcg gtc gct aag aaa gtg gtc gtc ccc att 48 Asp Met Gly Phe Glu Ala Ala Val Ala Lys Lys Val Val Val Pro Ile 1 5 10 15 acc ttc atg gtt ccc aac aga cct tct ggg ctt aca cag tcc gct ctt 96 Thr Phe Met Val Pro Asn Arg Pro Ser Gly Leu Thr Gln Ser Ala Leu 20 25 30 ctg gtg acc ggc cgg acc ttc cta atc aat gaa cat aca tgg tcc aat 144 Leu Val Thr Gly Arg Thr Phe Leu Ile Asn Glu His Thr Trp Ser Asn 35 40 45 ccc tcc tgg acc agc ttc aca atc cgc ggt gag gta cac act cgt gat 192 Pro Ser Trp Thr Ser Phe Thr Ile Arg Gly Glu Val His Thr Arg Asp 50 55 60 gag ccc ttc caa acg gtt cat ttc act cac cac ggt att ccc aca gat 240 Glu Pro Phe Gln Thr Val His Phe Thr His His Gly Ile Pro Thr Asp 65 70 75 80 ctg atg atg gta cgt ctc gga ccg ggc aat tct ttc cct aac aat cta 288 Leu Met Met Val Arg Leu Gly Pro Gly Asn Ser Phe Pro Asn Asn Leu 85 90 95 gac aag ttt gga ctt gac cag atg ccg gca cgc aac tcc cgt gtg gtt 336 Asp Lys Phe Gly Leu Asp Gln Met Pro Ala Arg Asn Ser Arg Val Val 100 105 110 ggc gtt tcg tcc agt tac gga aac ttc ttc ttc tct gga aat ttc ctc 384 Gly Val Ser Ser Ser Tyr Gly Asn Phe Phe Phe Ser Gly Asn Phe Leu 115 120 125 gga ttt gtt gat tcc atc acc tct gaa caa gga act tac gca aga ctc 432 Gly Phe Val Asp Ser Ile Thr Ser Glu Gln Gly Thr Tyr Ala Arg Leu 130 135 140 ttt agg tac agg gtg acg acc tac aaa gga tgg tgc ggc tcg gcc ctg 480 Phe Arg Tyr Arg Val Thr Thr Tyr Lys Gly Trp Cys Gly Ser Ala Leu 145 150 155 160 gtc tgt gag gcc ggt ggc gtc cga cgc atc att ggc ctg cat tct 525 Val Cys Glu Ala Gly Gly Val Arg Arg Ile Ile Gly Leu His Ser 165 170 175 <210> 638 <211> 175 <212> PRT <213> Seneca Valley Virus <400> 638 Asp Met Gly Phe Glu Ala Ala Val Ala Lys Lys Val Val Val Pro Ile 1 5 10 15 Thr Phe Met Val Pro Asn Arg Pro Ser Gly Leu Thr Gln Ser Ala Leu 20 25 30 Leu Val Thr Gly Arg Thr Phe Leu Ile Asn Glu His Thr Trp Ser Asn 35 40 45 Pro Ser Trp Thr Ser Phe Thr Ile Arg Gly Glu Val His Thr Arg Asp 50 55 60 Glu Pro Phe Gln Thr Val His Phe Thr His His Gly Ile Pro Thr Asp 65 70 75 80 Leu Met Met Val Arg Leu Gly Pro Gly Asn Ser Phe Pro Asn Asn Leu 85 90 95 Asp Lys Phe Gly Leu Asp Gln Met Pro Ala Arg Asn Ser Arg Val Val 100 105 110 Gly Val Ser Ser Ser Tyr Gly Asn Phe Phe Phe Ser Gly Asn Phe Leu 115 120 125 Gly Phe Val Asp Ser Ile Thr Ser Glu Gln Gly Thr Tyr Ala Arg Leu 130 135 140 Phe Arg Tyr Arg Val Thr Thr Tyr Lys Gly Trp Cys Gly Ser Ala Leu 145 150 155 160 Val Cys Glu Ala Gly Gly Val Arg Arg Ile Ile Gly Leu His Ser 165 170 175 <210> 639 <211> 549 <212> DNA <213> Swine Vesicular Disease <220> <221> CDS <222> (1)..(549) <220> <221> misc_feature <222> (1)..(549) <223> Swine_Vesicular_Disease_Virus_3C(wt) <400> 639 ggt cca gcg ttt gag ttc gcc gtg gcg atg atg aaa aga aac gcc agt 48 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ala Ser 1 5 10 15 aca gtg aaa act gag tac ggt gaa ttc acc atg ctt ggg att tac gac 96 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 agg tgg gcg gtg ttg cca cgc cat gcc aaa cct ggc ccc acc atc ttg 144 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 atg aac gac cag gta gtc gga gtg ttg gac gcc aag gaa cta gtt gat 192 Met Asn Asp Gln Val Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 aaa gat ggg acc aac ctg gaa ttg act ctc ttg aag ctc aac cgc aac 240 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 gag aag ttt aga gac atc agg gga ttc tta gca cga gag gag gtc gaa 288 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Arg Glu Glu Val Glu 85 90 95 gtg aac gaa gct gtc cta gca ata aac aca agt aaa ttc ccg aat atg 336 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 tac ata ccc gtg ggc cgg gta acc gac tat ggg ttc tta aat ctg ggt 384 Tyr Ile Pro Val Gly Arg Val Thr Asp Tyr Gly Phe Leu Asn Leu Gly 115 120 125 gga acc ccc acg aag aga atg ctc atg tac aat ttc cca act agg gca 432 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 ggc cag tgt ggg ggt gtc ctt atg tca aca ggg aaa gtc ctg gga ata 480 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 cat gta gga ggg aat gga cac caa ggg ttt tca gcg gca ctc ctc aga 528 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Arg 165 170 175 cac tac ttc aac gag gag cag 549 His Tyr Phe Asn Glu Glu Gln 180 <210> 640 <211> 183 <212> PRT <213> Swine Vesicular Disease <400> 640 Gly Pro Ala Phe Glu Phe Ala Val Ala Met Met Lys Arg Asn Ala Ser 1 5 10 15 Thr Val Lys Thr Glu Tyr Gly Glu Phe Thr Met Leu Gly Ile Tyr Asp 20 25 30 Arg Trp Ala Val Leu Pro Arg His Ala Lys Pro Gly Pro Thr Ile Leu 35 40 45 Met Asn Asp Gln Val Val Gly Val Leu Asp Ala Lys Glu Leu Val Asp 50 55 60 Lys Asp Gly Thr Asn Leu Glu Leu Thr Leu Leu Lys Leu Asn Arg Asn 65 70 75 80 Glu Lys Phe Arg Asp Ile Arg Gly Phe Leu Ala Arg Glu Glu Val Glu 85 90 95 Val Asn Glu Ala Val Leu Ala Ile Asn Thr Ser Lys Phe Pro Asn Met 100 105 110 Tyr Ile Pro Val Gly Arg Val Thr Asp Tyr Gly Phe Leu Asn Leu Gly 115 120 125 Gly Thr Pro Thr Lys Arg Met Leu Met Tyr Asn Phe Pro Thr Arg Ala 130 135 140 Gly Gln Cys Gly Gly Val Leu Met Ser Thr Gly Lys Val Leu Gly Ile 145 150 155 160 His Val Gly Gly Asn Gly His Gln Gly Phe Ser Ala Ala Leu Leu Arg 165 170 175 His Tyr Phe Asn Glu Glu Gln 180 <210> 641 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Nde-3C-F2 Primer <400> 641 catatgagtg gtgccccacc gac 23 <210> 642 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CCGATTCTAGACTCGAGTTA <400> 642 ccgattctag actcgagtta 20 <210> 643 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> T7 Primer <400> 643 taatacgact cactataggg 20 <210> 644 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> pSNAP-SR Primer <400> 644 cggatatagt tcctcctttc 20 <210> 645 <211> 90 <212> DNA <213> Artificial Sequence <220> <223> Delta 1D2A interrupter sequence <220> <221> misc_feature <222> (1)..(90) <223> Nucleotides 34-87 code for the 2A sequence <220> <221> CDS <222> (1)..(90) <400> 645 cay aag caa aag atc att gca cca gca aag cag ctt ctg aat ttt gac 48 His Lys Gln Lys Ile Ile Ala Pro Ala Lys Gln Leu Leu Asn Phe Asp 1 5 10 15 ctg ctc aag ttg gcc gga gac gtt gag tcc aac cct gga ccc 90 Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 20 25 30 <210> 646 <211> 30 <212> PRT <213> Artificial Sequence <220> <223> Synthetic Construct <400> 646 His Lys Gln Lys Ile Ile Ala Pro Ala Lys Gln Leu Leu Asn Phe Asp 1 5 10 15 Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 20 25 30 <210> 647 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Conserved 2A motif <220> <221> MISC_FEATURE <222> (2)..(2) <223> XAA is any naturally occurring amino acid <220> <221> MISC_FEATURE <222> (4)..(4) <223> XAA is any naturally occurring amino acid <400> 647 Asp Xaa Glu Xaa Asn Pro Gly Pro 1 5 <210> 648 <211> 558 <212> DNA <213> Gaussia princeps <220> <221> CDS <222> (1)..(558) <220> <221> misc_feature <222> (1)..(558) <223> GLuc <400> 648 atg gga gtc aaa gtt ctg ttt gcc ctg atc tgc atc gct gtg gcc gag 48 Met Gly Val Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 1 5 10 15 gcc aag ccc acc gag aac aac gaa gac ttc aac atc gtg gcc gtg gcc 96 Ala Lys Pro Thr Glu Asn Asn Glu Asp Phe Asn Ile Val Ala Val Ala 20 25 30 agc aac ttc gcg acc acg gat ctc gat gct gac cgc ggg aag ttg ccc 144 Ser Asn Phe Ala Thr Thr Asp Leu Asp Ala Asp Arg Gly Lys Leu Pro 35 40 45 ggc aag aag ctg ccg ctg gag gtg ctc aaa gag atg gaa gcc aat gcc 192 Gly Lys Lys Leu Pro Leu Glu Val Leu Lys Glu Met Glu Ala Asn Ala 50 55 60 cgg aaa gct ggc tgc acc agg ggc tgt ctg atc tgc ctg tcc cac atc 240 Arg Lys Ala Gly Cys Thr Arg Gly Cys Leu Ile Cys Leu Ser His Ile 65 70 75 80 aag tgc acg ccc aag atg aag aag ttc atc cca gga cgc tgc cac acc 288 Lys Cys Thr Pro Lys Met Lys Lys Phe Ile Pro Gly Arg Cys His Thr 85 90 95 tac gaa ggc gac aaa gag tcc gca cag ggc ggc ata ggc gag gcg atc 336 Tyr Glu Gly Asp Lys Glu Ser Ala Gln Gly Gly Ile Gly Glu Ala Ile 100 105 110 gtc gac att cct gag att cct ggg ttc aag gac ttg gag ccc atg gag 384 Val Asp Ile Pro Glu Ile Pro Gly Phe Lys Asp Leu Glu Pro Met Glu 115 120 125 cag ttc atc gca cag gtc gat ctg tgt gtg gac tgc aca act ggc tgc 432 Gln Phe Ile Ala Gln Val Asp Leu Cys Val Asp Cys Thr Thr Gly Cys 130 135 140 ctc aaa ggg ctt gcc aac gtg cag tgt tct gac ctg ctc aag aag tgg 480 Leu Lys Gly Leu Ala Asn Val Gln Cys Ser Asp Leu Leu Lys Lys Trp 145 150 155 160 ctg ccg caa cgc tgt gcg acc ttt gcc agc aag atc cag ggc cag gtg 528 Leu Pro Gln Arg Cys Ala Thr Phe Ala Ser Lys Ile Gln Gly Gln Val 165 170 175 gac aag atc aag ggg gcc ggt ggt gac taa 558 Asp Lys Ile Lys Gly Ala Gly Gly Asp 180 185 <210> 649 <211> 185 <212> PRT <213> Gaussia princeps <400> 649 Met Gly Val Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 1 5 10 15 Ala Lys Pro Thr Glu Asn Asn Glu Asp Phe Asn Ile Val Ala Val Ala 20 25 30 Ser Asn Phe Ala Thr Thr Asp Leu Asp Ala Asp Arg Gly Lys Leu Pro 35 40 45 Gly Lys Lys Leu Pro Leu Glu Val Leu Lys Glu Met Glu Ala Asn Ala 50 55 60 Arg Lys Ala Gly Cys Thr Arg Gly Cys Leu Ile Cys Leu Ser His Ile 65 70 75 80 Lys Cys Thr Pro Lys Met Lys Lys Phe Ile Pro Gly Arg Cys His Thr 85 90 95 Tyr Glu Gly Asp Lys Glu Ser Ala Gln Gly Gly Ile Gly Glu Ala Ile 100 105 110 Val Asp Ile Pro Glu Ile Pro Gly Phe Lys Asp Leu Glu Pro Met Glu 115 120 125 Gln Phe Ile Ala Gln Val Asp Leu Cys Val Asp Cys Thr Thr Gly Cys 130 135 140 Leu Lys Gly Leu Ala Asn Val Gln Cys Ser Asp Leu Leu Lys Lys Trp 145 150 155 160 Leu Pro Gln Arg Cys Ala Thr Phe Ala Ser Lys Ile Gln Gly Gln Val 165 170 175 Asp Lys Ile Lys Gly Ala Gly Gly Asp 180 185 <210> 650 <211> 558 <212> DNA <213> Gaussia princeps <220> <221> misc_feature <222> (1)..(558) <223> SGLuc <220> <221> CDS <222> (1)..(558) <400> 650 atg gga gtc aaa gtt ctg ttt gcc ctg atc tgc atc gct gtg gcc gag 48 Met Gly Val Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 1 5 10 15 gcc aag ccc acc gag aac aac gaa gac ttc aac atc gtg gcc gtg gcc 96 Ala Lys Pro Thr Glu Asn Asn Glu Asp Phe Asn Ile Val Ala Val Ala 20 25 30 agc aac ttt gcg acc acg gat ctc gat gct gac cga ggg aag ttg ccc 144 Ser Asn Phe Ala Thr Thr Asp Leu Asp Ala Asp Arg Gly Lys Leu Pro 35 40 45 ggc aag aag ctg ccg ctg gag gtg ctc aaa gag atg gaa gcc aat gcc 192 Gly Lys Lys Leu Pro Leu Glu Val Leu Lys Glu Met Glu Ala Asn Ala 50 55 60 cgg aaa gct ggc tgc acc agg ggc tgt ctg atc tgc ctg tcc cac atc 240 Arg Lys Ala Gly Cys Thr Arg Gly Cys Leu Ile Cys Leu Ser His Ile 65 70 75 80 aag tgc acg ccc aag atg aag aag tgg ctc cca gga cgc tgc cac acc 288 Lys Cys Thr Pro Lys Met Lys Lys Trp Leu Pro Gly Arg Cys His Thr 85 90 95 tac gaa ggc gac aaa gag tcc gca cag ggc ggc ata ggc gag gcg atc 336 Tyr Glu Gly Asp Lys Glu Ser Ala Gln Gly Gly Ile Gly Glu Ala Ile 100 105 110 gtc gat att cct gag att cct ggg ttc aag gac ttg gag cca atg gag 384 Val Asp Ile Pro Glu Ile Pro Gly Phe Lys Asp Leu Glu Pro Met Glu 115 120 125 cag ttc atc gca cag gtc gat ctg tgt gtg gac tgc aca act ggc tgc 432 Gln Phe Ile Ala Gln Val Asp Leu Cys Val Asp Cys Thr Thr Gly Cys 130 135 140 ctc aaa ggg ctt gcc aac gtg cag tgt tct gac ctg ctc aag aag tgg 480 Leu Lys Gly Leu Ala Asn Val Gln Cys Ser Asp Leu Leu Lys Lys Trp 145 150 155 160 ctg ccg caa cgc tgt gcg acc ttt gcc agc aag atc cag ggc cag gtg 528 Leu Pro Gln Arg Cys Ala Thr Phe Ala Ser Lys Ile Gln Gly Gln Val 165 170 175 gac aag atc aag ggg gcc ggt ggt gac taa 558 Asp Lys Ile Lys Gly Ala Gly Gly Asp 180 185 <210> 651 <211> 185 <212> PRT <213> Gaussia princeps <400> 651 Met Gly Val Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 1 5 10 15 Ala Lys Pro Thr Glu Asn Asn Glu Asp Phe Asn Ile Val Ala Val Ala 20 25 30 Ser Asn Phe Ala Thr Thr Asp Leu Asp Ala Asp Arg Gly Lys Leu Pro 35 40 45 Gly Lys Lys Leu Pro Leu Glu Val Leu Lys Glu Met Glu Ala Asn Ala 50 55 60 Arg Lys Ala Gly Cys Thr Arg Gly Cys Leu Ile Cys Leu Ser His Ile 65 70 75 80 Lys Cys Thr Pro Lys Met Lys Lys Trp Leu Pro Gly Arg Cys His Thr 85 90 95 Tyr Glu Gly Asp Lys Glu Ser Ala Gln Gly Gly Ile Gly Glu Ala Ile 100 105 110 Val Asp Ile Pro Glu Ile Pro Gly Phe Lys Asp Leu Glu Pro Met Glu 115 120 125 Gln Phe Ile Ala Gln Val Asp Leu Cys Val Asp Cys Thr Thr Gly Cys 130 135 140 Leu Lys Gly Leu Ala Asn Val Gln Cys Ser Asp Leu Leu Lys Lys Trp 145 150 155 160 Leu Pro Gln Arg Cys Ala Thr Phe Ala Ser Lys Ile Gln Gly Gln Val 165 170 175 Asp Lys Ile Lys Gly Ala Gly Gly Asp 180 185 <210> 652 <211> 657 <212> DNA <213> Hepatitis A virus <220> <221> CDS <222> (1)..(657) <220> <221> misc_feature <222> (1)..(657) <223> Hepatitis A 3C(G17P) <400> 652 tca act ttg gaa ata gca gga ctg gtt agg aag aac ttg gtt cag ttt 48 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 ccn gtt gga gag aag aat gga tgt gtg aga tgg gtt atg aat gcc ttg 96 Pro Val Gly Glu Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 gga gtg aaa gat gat tgg ctg ctt gtg cct tcc cat gct tat aaa ttt 144 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 gag aaa gat tat gaa atg atg gag ttt tat ttt aat aga ggt gga act 192 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 tac tat tca att tca gct ggt aat gtt gtt att caa tct ttg gat gtg 240 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 gga ttc cag gat gtt gtt ctg atg aag gtt cct aca att cct aag ttt 288 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 aga gat att act cag cat ttt att aag aaa ggg gat gtg cct aga gct 336 Arg Asp Ile Thr Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 ttg aat cgc ctg gca aca tta gtg aca act gta aat gga acc cct atg 384 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Met 115 120 125 tta att tct gag ggc cca cta aag atg gaa gag aaa gct act tat gtt 432 Leu Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 cat aag aaa aat gat ggt aca aca gtt gat tta act gtg gat cag gca 480 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 tgg aga gga aaa ggc gaa ggt ctt cct gga atg tgt ggt ggg gcc ttg 528 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 gtt tca tcg aat caa tct ata cag aat gca atc ttg ggc atc cat gtt 576 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 gct gga gga aat tca att ctt gtt gca aaa ttg gtt act caa gaa atg 624 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 ttc caa aat att gat aag aaa att gaa agt cag 657 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 653 <211> 219 <212> PRT <213> Hepatitis A virus <400> 653 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 Pro Val Gly Glu Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 Arg Asp Ile Thr Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Met 115 120 125 Leu Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 654 <211> 657 <212> DNA <213> Hepatitis A virus <220> <221> CDS <222> (1)..(657) <220> <221> misc_feature <222> (1)..(657) <223> Hepatitis A 3C(V18P) <400> 654 tca act ttg gaa ata gca gga ctg gtt agg aag aac ttg gtt cag ttt 48 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 gga ccn gga gag aag aat gga tgt gtg aga tgg gtt atg aat gcc ttg 96 Gly Pro Gly Glu Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 gga gtg aaa gat gat tgg ctg ctt gtg cct tcc cat gct tat aaa ttt 144 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 gag aaa gat tat gaa atg atg gag ttt tat ttt aat aga ggt gga act 192 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 tac tat tca att tca gct ggt aat gtt gtt att caa tct ttg gat gtg 240 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 gga ttc cag gat gtt gtt ctg atg aag gtt cct aca att cct aag ttt 288 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 aga gat att act cag cat ttt att aag aaa ggg gat gtg cct aga gct 336 Arg Asp Ile Thr Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 ttg aat cgc ctg gca aca tta gtg aca act gta aat gga acc cct atg 384 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Met 115 120 125 tta att tct gag ggc cca cta aag atg gaa gag aaa gct act tat gtt 432 Leu Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 cat aag aaa aat gat ggt aca aca gtt gat tta act gtg gat cag gca 480 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 tgg aga gga aaa ggc gaa ggt ctt cct gga atg tgt ggt ggg gcc ttg 528 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 gtt tca tcg aat caa tct ata cag aat gca atc ttg ggc atc cat gtt 576 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 gct gga gga aat tca att ctt gtt gca aaa ttg gtt act caa gaa atg 624 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 ttc caa aat att gat aag aaa att gaa agt cag 657 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215 <210> 655 <211> 219 <212> PRT <213> Hepatitis A virus <400> 655 Ser Thr Leu Glu Ile Ala Gly Leu Val Arg Lys Asn Leu Val Gln Phe 1 5 10 15 Gly Pro Gly Glu Lys Asn Gly Cys Val Arg Trp Val Met Asn Ala Leu 20 25 30 Gly Val Lys Asp Asp Trp Leu Leu Val Pro Ser His Ala Tyr Lys Phe 35 40 45 Glu Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe Asn Arg Gly Gly Thr 50 55 60 Tyr Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gln Ser Leu Asp Val 65 70 75 80 Gly Phe Gln Asp Val Val Leu Met Lys Val Pro Thr Ile Pro Lys Phe 85 90 95 Arg Asp Ile Thr Gln His Phe Ile Lys Lys Gly Asp Val Pro Arg Ala 100 105 110 Leu Asn Arg Leu Ala Thr Leu Val Thr Thr Val Asn Gly Thr Pro Met 115 120 125 Leu Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Lys Ala Thr Tyr Val 130 135 140 His Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Thr Val Asp Gln Ala 145 150 155 160 Trp Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cys Gly Gly Ala Leu 165 170 175 Val Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Leu Gly Ile His Val 180 185 190 Ala Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Val Thr Gln Glu Met 195 200 205 Phe Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln 210 215

Claims (20)

  1. 변형된 피코르나바이러스 3C 프로테아제(picornavirus 3C protease)를 코딩하는 단리된 폴리뉴클레오타이드로서,
    상기 변형된 피코르나바이러스 3C 프로테아제는 야생형 구제역 바이러스(Foot-and-Mouth Disease virus, FMDV) 3C 프로테아제의 변경된 2차 구조 및 16 내지 25, 99, 100 및 115 내지 130 위치에 상응하는 하나 이상의 아미노산 위치(들)에 위치한 하나 이상의 아미노산 치환(들)을 포함하는 것이고,
    상기 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 상기 단리된 폴리뉴클레오타이드는, 숙주 세포에서 형질전환되고 공동-발현될 때, 대조군 피코르나바이러스 3C 프로테아제로 형질전환되고 공동-발현된 숙주 세포에서 나타나는 P1 전구체 폴리펩타이드 형질전환 유전자의 발현양과 비교하여, P1 전구체 폴리펩타이드의 형질전환 유전자의 발현을 증가시키고,
    상기 하나 이상의 상응하는 아미노산 위치(들)는 상기 변형된 피코르나바이러스 3C 프로테아제와 하나 이상의 상기 야생형 FMDV 3C 프로테아제(들)의 정렬에 의해 동정되는 것이고,
    상기 변형된 피코르나바이러스 3C 프로테아제는 하나 이상의 아미노산 치환을 갖는 변형된 FMDV 3C 프로테아제를 배제한 것으로서, 상기 하나 이상의 아미노산 치환은 위치 125에서의 아미노산 치환, 위치 126에서의 아미노산 치환, 위치 127에서의 아미노산 치환, 위치 128에서의 아미노산 치환, 위치 129에서의 아미노산 치환, 위치 130에서의 아미노산 치환 및 이들의 조합들로 이루어지는 군으로부터 선택되는 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  2. 제 1 항에 있어서,
    상기 변경된 2차 구조가 하기로부터 선택되고:
    A1-B1 β 시트,
    F1 β 가닥과 A2 β 가닥 사이의 루프, 및
    A2-B2 β 시트;
    상기 A1-B1 β 시트를 변경하는 상기 하나 이상의 아미노산 치환(들)은 야생형 FMDV 3C 프로테아제의 아미노산 위치 16 내지 25 중 하나 이상에 상응하는 위치(들)에 위치하고,
    상기 F1 β 가닥과 A2 β 가닥 사이의 루프를 변경하는 상기 하나 이상의 아미노산 치환(들)은 야생형 FMDV 3C 프로테아제의 아미노산 위치 99 및 100의 하나 이상에 상응하는 위치(들)에 위치하고,
    상기 A2-B2 β 시트를 변경하는 상기 하나 이상의 아미노산 치환(들)은 야생형 FMDV 3C 프로테아제의 아미노산 위치 115 내지 130 중 하나 이상에 상응하는 위치(들)에 위치하는 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  3. 제 1 항 또는 제 2 항에 있어서,
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제이고:
    I22 및 L23 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 FMDV 3C 프로테아제,
    V22 및 C23 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 소 비염 A(Bovine rhinitis A) 바이러스 3C 프로테아제,
    V22 및 R23 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 소 비염 B(Bovine rhinitis B) 3C 프로테아제, 및
    Y22 및 C23 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 말 비염(Equine rhinitis) 3C 프로테아제;
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 변형된 엔테로바이러스 A(Enterovirus A) 3C 프로테아제이고:
    Q19 및 Q20 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 인간 콕사키바이러스(Human coxsackievirus A) 바이러스, 및
    Q19 및 T20 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 인간 엔테로바이러스 A71(Human enterovirus A71) 3C 프로테아제;
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 변형된 엔테로바이러스 B(Enterovirus B) 3C 프로테아제이고:
    K19 및 T20 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 인간 콕사키바이러스 B1(Human coxsackievirus B1) 3C 프로테아제, 및 K19 및 T20 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 인간 콕사키바이러스 B3(Human coxsackievirus B3) 3C 프로테아제;
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 변형된 엔테로바이러스(Enterovirus) 3C 프로테아제이고:
    T19 및 T20 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 콕사키바이러스 A1(coxsackievirus A1) 3C 프로테아제, 및
    T19 및 T20 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 인간 폴리오바이러스(Human poliovirus) 3C 프로테아제;
    상기 변형된 피코르나바이러스 3C 프로테아제는 T19 및 T20 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 리노바이러스 A(Rhinovirus A) 3C 프로테아제이고;
    상기 변형된 피코르나바이러스 3C 프로테아제는 T19 및 T20 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 리노바이러스 A(Rhinovirus A) 3C 프로테아제이고,
    상기 변형된 피코르나바이러스 3C 프로테아제는 G19 및 T20 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 A형 간염 바이러스(Hepatitis A virus) 3C 프로테아제인 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  4. 제 1 항 내지 제 3 항 중 어느 한 항에 있어서,
    상기 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제는 하기 중 하나 이상으로부터 선택되고:
    V124에서 아미노산 치환을 포함하는 변형된 FMDV 3C 프로테아제,
    F120 및 L123 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 소 비염 A(Bovine rhinitis A) 바이러스 3C 프로테아제,
    V123 및 L124 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 소 비염 B(Bovine rhinitis B) 3C 프로테아제, 및
    A125 및 T127 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 말 비염(Equine rhinitis) 3C 프로테아제;
    상기 변형된 엔테로바이러스(Enterovirus) 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 엔테로바이러스 A(Enterovirus A) 3C 프로테아제이고:
    M112, V114, M109, I104 및 L102 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 인간 콕사키바이러스 A(Human coxsackievirus A) 바이러스, 및
    T106, M109 및 I104 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 인간 엔테로바이러스 A71(Human enterovirus A71) 3C 프로테아제;
    상기 변형된 엔테로바이러스(Enterovirus) 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 엔테로바이러스 B(Enterovirus B) 3C 프로테아제이고:
    M112, I114, F109, I104 및 L102 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 인간 콕사키바이러스 B1(Human coxsackievirus B1), 및
    M112, I114, F109, I104 및 L102 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 인간 콕사키바이러스 B3(Human coxsackievirus B3);
    상기 변형된 엔테로바이러스(Enterovirus) 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 엔테로바이러스 V(Enterovirus V) 3C 프로테아제이고:
    M112, I114, F109, V104 및 L102 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 콕사키바이러스 A1(Coxsackievirus A1) 3C 프로테아제, 및
    V104 및 L102 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 인간 폴리오바이러스(Human poliovirus) 3C 프로테아제;
    상기 변형된 엔테로바이러스(Enterovirus) 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 리노바이러스(Rhinovirus) 3C 프로테아제이고:
    Q108, T112, I114, L102 및 L104 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 리노바이러스 A(Rhinovirus A) 3C 프로테아제, 및
    I112 및 E114 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 리노바이러스 B(Rhinovirus B) 3C 프로테아제;
    상기 변형된 헤파토바이러스(Hepatovirus)는 M128, I130, T121 및 L119 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환들을 포함하는 변형된 A형 간염 바이러스(Hepatitis A virus) 3C 프로테아제인 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  5. 제 1 항 내지 제 4 항 중 어느 한 항에 있어서,
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제이고:
    위치 T100에서 아미노산 치환을 포함하는 변형된 FMDV 3C 프로테아제,
    위치 T96에서 아미노산 치환을 포함하는 변형된 소 비염 A(Bovine rhinitis A) 바이러스 3C 프로테아제,
    위치 T97에서 아미노산 치환을 포함하는 변형된 소 비염 B(Bovine rhinitis B) 3C 프로테아제, 및
    위치 V100에서 아미노산 치환을 포함하는 변형된 말 비염(Equine rhinitis) 3C 프로테아제;
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 변형된 엔테로바이러스 A(Enterovirus A) 3C 프로테아제이고:
    위치 T87에서 아미노산 치환을 포함하는 변형된 인간 콕사키바이러스 A(Human coxsackievirus A) 바이러스, 및
    위치 T87에서 아미노산 치환을 포함하는 변형된 인간 엔테로바이러스 A71(Human enterovirus A71) 3C 프로테아제;
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 변형된 엔테로바이러스 B(Enterovirus B) 3C 프로테아제이고:
    위치 R87에서 아미노산 치환을 포함하는 변형된 인간 콕사키바이러스 B1(Human coxsackievirus B1), 및
    위치 R87에서 아미노산 치환을 포함하는 변형된 인간 콕사키바이러스 B3(Human coxsackievirus B3);
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 변형된 엔테로바이러스 C(Enterovirus C) 3C 프로테아제이고:
    위치 R87에서 아미노산 치환을 포함하는 변형된 콕사키바이러스 A1(Coxsackievirus A1) 3C 프로테아제, 및
    위치 R87에서 아미노산 치환을 포함하는 변형된 인간 폴리오바이러스(Human poliovirus) 3C 프로테아제;
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 변형된 리노바이러스(Rhinovirus) 3C 프로테아제이고:
    R87, R88 및 G88에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 리노바이러스 A(Rhinovirus A) 3C 프로테아제, 및
    R87 및 K88 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 리노바이러스 B(Rhinovirus B) 3C 프로테아제;
    상기 변형된 피코르나바이러스 3C 프로테아제는 T100 및 Q101 중에서 선택되는 하나 이상의 위치(들)에서 하나 이상의 아미노산 치환(들)을 포함하는 변형된 A형 간염 바이러스(Hepatitis A virus) 3C 프로테아제인 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  6. 제 1 항 내지 제 5 항 중 어느 한 항에 있어서,
    상기 야생형 FMDV 3C 프로테아제는 서열번호 2, 4, 6, 8, 10, 12, 14, 16, 18 및 20 중에서 선택되는 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  7. 제 1 항 내지 제 6 항 중 어느 한 항에 있어서,
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제이고:
    서열번호 2, 4, 6, 8, 10, 12, 14, 16, 18 및 20 중에서 선택되는 하나 이상의 야생형 FMDV 3C 프로테아제와 적어도 95%의 상동성을 갖는 변형된 FMDV 3C 프로테아제,
    서열번호 422 및 434 중에서 선택되는 하나 이상의 야생형 소 비염(Bovine rhinitis) 3C 프로테아제와 적어도 95%의 상동성을 갖는 변형된 소 비염(Bovine rhinitis) 3C 프로테아제, 및
    서열번호 444의 야생형 말 비염(Equine rhinitis) 3C 프로테아제와 적어도 95%의 상동성을 갖는 변형된 말 비염(Equine rhinitis) 3C 프로테아제;
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중에서 선택되는 서열과 적어도 95%의 상동성을 갖는 변형된 엔테로바이러스 A(Enterovirus A) 3C 프로테아제이고:
    서열번호 478의 야생형 인간 콕사키바이러스 A10(Human coxsackievirus A10) 3C 프로테아제, 및
    서열번호 536의 야생형 인간 엔테로바이러스 A71(Human enterovirus A71) 3C 프로테아제;
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중에서 선택되는 서열과 적어도 95%의 상동성을 갖는 변형된 엔테로바이러스 B(Enterovirus B) 3C 프로테아제이고:
    서열번호 494의 야생형 인간 콕사키바이러스 B1(Human coxsackievirus B1) 3C 프로테아제, 및
    서열번호 510의 야생형 인간 콕사키바이러스 B3(Human coxsackievirus B3) 3C 프로테아제;
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중에서 선택되는 서열과 적어도 95%의 상동성을 갖는 변형된 엔테로바이러스 C(Enterovirus C) 3C 프로테아제이고:
    서열번호 462의 야생형 인간 콕사키바이러스 A1(Human coxsackievirus A1) 3C 프로테아제, 및
    서열번호 552, 566 및 580 중에서 선택되는 야생형 인간 폴리오바이러스(Human poliovirus) 3C 프로테아제;
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중에서 선택되는 서열과 적어도 95%의 상동성을 갖는 변형된 리노바이러스(Rhinovirus)이고:
    서열번호 604, 618 및 620 중에서 선택되는 야생형 인간 리노바이러스 A(Human rhinovirus A) 3C 프로테아제, 및
    서열번호 594의 야생형 리노바이러스 B(Rhinovirus B) 3C 프로테아제;
    상기 변형된 피코르나바이러스 3C 프로테아제는 서열번호 538의 야생형 인간 간염(Human hepatitis) 3C 프로테아제와 적어도 95%의 상동성을 갖는 변형된 A형 간염 바이러스(Hepatitis A virus) 3C 프로테아제인 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  8. 제 1 항 내지 제 7 항 중 어느 한 항에 있어서,
    상기 변형된 피코르나바이러스 3C 프로테아제는 하기 중 하나 이상으로부터 선택되는 변형된 아프토바이러스(Aphthovirus) 3C 프로테아제이고:
    서열번호 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 178, 180, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 302, 304, 306, 308, 310, 312, 314, 316, 318, 320, 322, 324, 326, 328, 330, 332, 334, 336, 338, 340, 342, 344, 346, 348, 350, 352, 354, 356, 358 및 360 중에서 선택되는 변형된 FMDV 3C 프로테아제,
    서열번호 412, 414, 416, 418, 420, 424, 426, 428, 430 및 432 중에서 선택되는 변형된 소 리노바이러스(Bovine rhinovirus) 3C 프로테아제, 및
    서열번호 436, 438, 440, 442 및 446 중에서 선택되는 변형된 말 리노바이러스(Equine rhinovirus) 3C 프로테아제;
    상기 변형된 피코르나바이러스 3C 프로테아제가 서열번호 464, 466, 468, 470, 472, 474, 476, 512, 514, 516, 518, 520, 522 및 524 중에서 선택되는 변형된 엔테로바이러스 A(Enterovirus A) 3C 프로테아제이고;
    상기 변형된 피코르나바이러스 3C 프로테아제가 서열번호 480, 482, 484, 486, 488, 490, 492, 496, 498, 500, 502, 504, 506 및 508 중에서 선택되는 변형된 엔테로바이러스 B(Enterovirus B) 3C 프로테아제이고;
    상기 변형된 피코르나바이러스 3C 프로테아제는 서열번호 448, 450, 452, 454, 456, 458, 460 540, 542, 544, 546, 548, 550, 554, 556, 558, 560, 562, 564, 568, 570, 572, 574, 576 및 578 중에서 선택되는 엔테로바이러스 C(Enterovirus C) 3C 프로테아제이고;
    상기 변형된 피코르나바이러스 3C 프로테아제는 서열번호 528, 530, 532, 534, 536, 652 및 654 중에서 선택되는 변형된 A형 간염 바이러스(Hepatitis A virus) 3C 프로테아제이고;
    상기 변형된 피코르나바이러스 3C 프로테아제는,
    서열번호 596, 598, 600, 602, 604, 606, 608, 610, 612, 614, 616, 618, 620, 622, 624, 626, 628, 630, 632 및 634 중에서 선택되는 변형된 리노바이러스 A(Rhinovirus A) 3C 프로테아제, 및
    서열번호 582, 584, 586, 588, 590 및 592 중에서 선택되는 변형된 리노바이러스 B(Rhinovirus B) 3C 프로테아제
    중에서 선택되는 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  9. 제 1 항 내지 제 8 항 중 어느 한 항에 있어서,
    상기 폴리뉴클레오타이드는, 숙주 세포에서 형질전환되고 공동-발현될 때, 대조군 피코르나바이러스 3C 프로테아제를 코딩하는 폴리뉴클레오타이드로 형질전환되고 공동-발현된 숙주 세포에서 단백질 분해로 절단된 eIF4A1의 발현양과 비교하여, 단백질 분해로 절단된 eIF4A1의 발? 양을 증가 또는 감소시키는 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  10. 제 1 항 내지 제 9 항 중 어느 한 항에 있어서,
    상기 하나 이상의 아미노산 치환(들)은 프롤린에 의한 치환을 포함하는 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  11. 제 1 항 내지 제 10 항 중 어느 한 항에 있어서,
    P1 전구체 폴리펩타이드를 코딩하는 적어도 하나의 폴리뉴클레오타이드 서열을 추가로 포함하는,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  12. 제 1 항 내지 제 11 항 중 어느 한 항의 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 폴리뉴클레오타이드를 포함하는, 벡터.
  13. 제 12 항의 벡터를 포함하는, 숙주 세포.
  14. 제 1 항 내지 제 13 항 중 어느 한 항의 변형된 피코르나바이러스 3C 프로테아제를 코딩하는 폴리뉴클레오타이드 또는 벡터, 및 상기 변형된 피코르나바이러스 3C 프로테아제에 의한 단백질 분해에 적합한 약학적으로 허용 가능한 부형제, 보조제, 완충제 또는 용액을 포함하는, 조성물.
  15. 피코르나바이러스 P1 전구체 폴리펩타이드를 피코르나바이러스 바이러스 단백질 및/또는 바이러스-유사 입자(Virus-Like Particles: VLPs)로 가공하는 방법으로서,
    제 13 항의 숙주세포를 적합한 배지에서 배양하는 것; 및
    VP0, VP1, VP2, VP3 및 VP4 및/또는 VLPs 중에서 선택되는 적어도 하나의바이러스 단백질을 회수하는 것,
    을 포함하는, 방법.
  16. 제 1 항 내지 제 15 항 중 어느 한 항에 있어서,
    상기 정렬은 기본 매개변수를 갖는 블로섬 62(Blossum 62)를 이용하여 클론 매니저 9, 버전 9.4(Clone Manager 9, Version 9.4, 1, January 2015)를 사용하여 결정되는 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  17. 제 1 항 내지 제 16 항 중 어느 한 항에 있어서,
    상기 하나 이상의 아미노산 치환은 비-보존적 치환인 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  18. 제 1 항 내지 제 17 항 중 어느 한 항에 있어서,
    상기 하나 이상의 아미노산 치환(들)은 야생형 구제역 바이러스(FMDV) 3C 프로테아제의 16 내지 25 위치에 상응하는 하나 이상의 아미노산 위치(들)에 위치한 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  19. 제 1 항 내지 제 18 항 중 어느 한 항에 있어서,
    상기 하나 이상의 아미노산 치환(들)은 야생형 구제역 바이러스(FMDV) 3C 프로테아제의 99 및 100 위치에 상응하는 하나 이상의 아미노산 위치(들)에 위치한 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
  20. 제 1 항 내지 제 19 항 중 어느 한 항에 있어서,
    상기 하나 이상의 아미노산 치환(들)은 야생형 구제역 바이러스(FMDV) 3C 프로테아제의 115 내지 130 위치에 상응하는 하나 이상의 아미노산 위치(들)에 위치한 것인,
    변형된 피코르나바이러스 3C 프로테아제를 코딩하는 단리된 폴리뉴클레오타이드.
KR1020197004446A 2018-05-22 2018-09-20 변형된 피코르나바이러스 3c 프로테아제 및 그의 방법 KR102277529B1 (ko)

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