KR20190128746A - 비율계량 형광 화상화 방법 - Google Patents

비율계량 형광 화상화 방법 Download PDF

Info

Publication number
KR20190128746A
KR20190128746A KR1020197033078A KR20197033078A KR20190128746A KR 20190128746 A KR20190128746 A KR 20190128746A KR 1020197033078 A KR1020197033078 A KR 1020197033078A KR 20197033078 A KR20197033078 A KR 20197033078A KR 20190128746 A KR20190128746 A KR 20190128746A
Authority
KR
South Korea
Prior art keywords
image
fluorescence
fluorescence intensity
cancer
ratio
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
KR1020197033078A
Other languages
English (en)
Korean (ko)
Inventor
알렉 하루투니언
지저스 이 곤잘레즈
Original Assignee
아벨라스 바이오사이언시즈 인코포레이티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 아벨라스 바이오사이언시즈 인코포레이티드 filed Critical 아벨라스 바이오사이언시즈 인코포레이티드
Publication of KR20190128746A publication Critical patent/KR20190128746A/ko
Abandoned legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent materials, e.g. electroluminescent or chemiluminescent
    • C09K11/06—Luminescent materials, e.g. electroluminescent or chemiluminescent containing organic luminescent materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/00002—Operational features of endoscopes
    • A61B1/00004—Operational features of endoscopes characterised by electronic signal processing
    • A61B1/00009—Operational features of endoscopes characterised by electronic signal processing of image signals during a use of endoscope
    • A61B1/000096—Operational features of endoscopes characterised by electronic signal processing of image signals during a use of endoscope using artificial intelligence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/03—Peptides having up to 20 amino acids in an undefined or only partially defined sequence; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent materials, e.g. electroluminescent or chemiluminescent
    • C09K11/06—Luminescent materials, e.g. electroluminescent or chemiluminescent containing organic luminescent materials
    • C09K11/07—Luminescent materials, e.g. electroluminescent or chemiluminescent containing organic luminescent materials having chemically-interreactive components, e.g. reactive chemiluminescent compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/136—Segmentation; Edge detection involving thresholding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Theoretical Computer Science (AREA)
  • Medical Informatics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Radiology & Medical Imaging (AREA)
  • Organic Chemistry (AREA)
  • Materials Engineering (AREA)
  • Molecular Biology (AREA)
  • Quality & Reliability (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Signal Processing (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Optics & Photonics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Artificial Intelligence (AREA)
KR1020197033078A 2017-04-07 2018-04-05 비율계량 형광 화상화 방법 Abandoned KR20190128746A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762483004P 2017-04-07 2017-04-07
US62/483,004 2017-04-07
PCT/US2018/026336 WO2018187629A1 (en) 2017-04-07 2018-04-05 Ratiometric fluorescence imaging methods

Publications (1)

Publication Number Publication Date
KR20190128746A true KR20190128746A (ko) 2019-11-18

Family

ID=63712695

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020197033078A Abandoned KR20190128746A (ko) 2017-04-07 2018-04-05 비율계량 형광 화상화 방법

Country Status (7)

Country Link
US (1) US11293868B2 (https=)
EP (2) EP3610243A4 (https=)
JP (2) JP2020519851A (https=)
KR (1) KR20190128746A (https=)
CN (1) CN110741242B (https=)
EA (1) EA201992252A1 (https=)
WO (1) WO2018187629A1 (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018187629A1 (en) 2017-04-07 2018-10-11 Avelas Biosciences, Inc. Ratiometric fluorescence imaging methods
SE544735C2 (en) * 2018-11-09 2022-11-01 Mm18 Medical Ab Method for identification of different categories of biopsy sample images
US20220167853A1 (en) * 2019-03-28 2022-06-02 Koite Health Oy Method for plaque detection
EP4136616B1 (en) * 2020-04-14 2024-11-06 SurgVision GmbH Verification of segmentation of luminescence images limited to analysis regions thereof
EP3907497B1 (en) * 2020-05-08 2023-08-02 Leica Microsystems CMS GmbH Apparatus and method for displaying and/or printing images of a specimen including a fluorophore
CN115697177A (zh) * 2020-07-20 2023-02-03 直观外科手术操作公司 基于图像确定发荧光物质的性质
JP2023542867A (ja) 2020-09-11 2023-10-12 グリンプス バイオ， インコーポレイテッド 疾患の検出／診断、病期分類、モニタリング及び処置のための生体外でのプロテアーゼ活性検出
WO2022056642A1 (en) * 2020-09-18 2022-03-24 Stryker European Operations Limited Systems and methods for fluorescence visualization
JP2023550687A (ja) * 2020-10-13 2023-12-05 アベラス アクイジション コーポレーション 選択的送達分子を使用する方法
CN112432934B (zh) * 2020-11-05 2021-07-06 北京中科生仪科技有限公司 发射光检测方法
WO2022147423A1 (en) * 2020-12-30 2022-07-07 Stryker Corporation Systems and methods of dual fluorophore ratiometric imaging
CN115575358B (zh) * 2021-06-21 2024-05-24 北京大学 一种对单分子荧光共振能量转移信号进行分析的方法
CN113896708B (zh) * 2021-10-29 2024-03-12 南京碳硅人工智能生物医药技术研究院有限公司 甲醛荧光探针的设计、合成及活性研究
DE102021134368B4 (de) 2021-12-22 2023-09-21 Hochschule Reutlingen Körperschaft des öffentlichen Rechts Vorrichtung und Verfahren zur Detektion von markierten Tumorzellen eines Gewebes in einer strömenden Flüssigkeit
CN114533148B (zh) * 2022-02-15 2023-06-16 佳木斯大学 一种消化内科用于胃癌检测的取样系统
EP4275590A1 (en) * 2022-05-13 2023-11-15 Leica Instruments (Singapore) Pte Ltd Data processing device and computer-implemented method combining two images and an overlay color using a uniform color space
WO2024155928A1 (en) * 2023-01-19 2024-07-25 Marquette University Systems, methods, and media for multimodal data collection and analysis for cancer screening
US20250072739A1 (en) * 2023-09-01 2025-03-06 Karl Storz Imaging, Inc. Single chip camera head for multi-spectral imaging and methods of using the same
PL448268A1 (pl) * 2024-04-11 2025-10-13 Urteste Spółka Akcyjna Związek - marker diagnostyczny guza mózgu, sposób wykrywania aktywności enzymatycznej, sposób diagnozowania guza mózgu, zestaw zawierający taki związek oraz zastosowania takiego związku i sposób leczenia guza mózgu

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4496542A (en) 1981-03-30 1985-01-29 Usv Pharmaceutical Corporation N-substituted-amido-amino acids
US4659839A (en) 1984-10-10 1987-04-21 Mallinckrodt, Inc. Coupling agents for radiolabeled antibody fragments
JP2001017379A (ja) 1999-07-09 2001-01-23 Fuji Photo Film Co Ltd 蛍光診断装置
CA2491703A1 (en) * 2002-07-09 2004-01-15 Medispectra, Inc. Methods and apparatus for characterization of tissue samples
JP4568840B2 (ja) 2004-12-28 2010-10-27 国立大学法人金沢大学 アルツハイマー病の検査方法
US8078265B2 (en) 2006-07-11 2011-12-13 The General Hospital Corporation Systems and methods for generating fluorescent light images
JP5487534B2 (ja) 2007-04-20 2014-05-07 株式会社ニコン 画像解析方法と、蛍光検出装置
CA2740602C (en) * 2007-10-19 2017-05-02 Visen Medical, Inc. Imaging systems featuring waveguiding compensation
US20090226059A1 (en) * 2008-02-12 2009-09-10 Richard Levenson Tissue Processing And Assessment
JP5338573B2 (ja) * 2009-08-31 2013-11-13 ソニー株式会社 蛍光像取得方法、蛍光像取得装置及び蛍光像取得プログラム
CA2841249C (en) * 2011-07-29 2023-09-26 Avelas Biosciences, Inc. Selective delivery molecules and methods of use
US11573235B2 (en) * 2012-03-30 2023-02-07 Leica Microsystems Cms Gmbh Methods for generating an image of a biological sample
CN107209118B (zh) * 2014-09-29 2021-05-28 史赛克欧洲运营有限公司 在自体荧光存在下生物材料中目标荧光团的成像
US10385380B2 (en) * 2014-10-02 2019-08-20 The Regents Of The University Of California Personalized protease assay to measure protease activity in neoplasms
WO2016148657A1 (en) 2015-03-19 2016-09-22 Histoindex Pte Ltd Method of detecting fibrous tissue in a biological specimen using co-localized images generated by second harmonic generation and two photon emission
CN108369734B (zh) * 2015-12-10 2022-04-26 凯杰有限公司 数字图像中的对象分类方法、系统及计算机可读介质
US10279585B2 (en) * 2017-01-31 2019-05-07 Xerox Corporation Method and system for aligning ejectors that eject clear materials in a printer
WO2018187629A1 (en) 2017-04-07 2018-10-11 Avelas Biosciences, Inc. Ratiometric fluorescence imaging methods
US10724956B1 (en) * 2019-02-01 2020-07-28 Essen Instruments, Inc. Spectral unmixing

Also Published As

Publication number Publication date
EP3610243A4 (en) 2020-12-16
EP3876196A1 (en) 2021-09-08
JP2020519851A (ja) 2020-07-02
EA201992252A1 (ru) 2020-04-02
US20200150041A1 (en) 2020-05-14
CN110741242A (zh) 2020-01-31
EP3610243A1 (en) 2020-02-19
JP2023102271A (ja) 2023-07-24
WO2018187629A1 (en) 2018-10-11
US11293868B2 (en) 2022-04-05
CN110741242B (zh) 2023-07-07

Similar Documents

Publication Publication Date Title
CN110741242B (zh) 比率荧光成像方法
US9695251B2 (en) Activatable cell penetrating peptides with quenched fluorophores
US9999687B2 (en) Fluorescent imaging agents
Gao et al. Fluorescent chemical probes for accurate tumor diagnosis and targeting therapy
ES2861528T3 (es) Colorantes del NIR dirigidos al PSMA y sus usos
CA3128911C (en) Selective delivery molecules and methods of use
CN110199195B (zh) Psma靶向的nir染料及其用途
US20040022731A1 (en) In vivo imaging of apoptosis
CN101896208B (zh) 眼部细胞凋亡荧光标记物
JP2018521994A (ja) 治療用ペプチドおよびそれらの使用方法
AU2022207469B2 (en) Methods and compositions for visualizing a ureter in a surgical procedure
Nagai et al. Design of a dendrimer with a matrix metalloproteinase-responsive fluorescence probe and a tumor-homing peptide for metastatic tumor cell imaging in the lymph node
JP2026035653A (ja) フルオロフォア標識ｕｐａｒ標的化ペプチドコンジュゲートを含む組成物
HK40051643A (en) Ratiometric fluorescence imaging methods
Uddin et al. Rational design of peptide ligands based on knob–socket protein packing model using CD13 as a prototype receptor
EP3999123A1 (en) A receptor-targeting conjugate with an effective pharmacokinetic profile
Fields et al. Imaging matrix metalloproteinase activity implicated in breast cancer progression

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

PC1904 Unpaid initial registration fee

St.27 status event code: A-2-2-U10-U14-oth-PC1904

St.27 status event code: N-2-6-B10-B12-nap-PC1904