JP2020519851A - レシオメトリック蛍光画像化方法 - Google Patents

レシオメトリック蛍光画像化方法 Download PDF

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Publication number
JP2020519851A
JP2020519851A JP2019553092A JP2019553092A JP2020519851A JP 2020519851 A JP2020519851 A JP 2020519851A JP 2019553092 A JP2019553092 A JP 2019553092A JP 2019553092 A JP2019553092 A JP 2019553092A JP 2020519851 A JP2020519851 A JP 2020519851A
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image
fluorescence
fluorescence intensity
cancer
threshold
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Japanese (ja)
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JP2020519851A5 (https=
Inventor
ハルトゥーニアン，アレック
ゴンザレス，ジーザス，イー．
Original Assignee
アベラス バイオサイエンシーズ，インク．
アベラス バイオサイエンシーズ，インク．
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Publication of JP2020519851A publication Critical patent/JP2020519851A/ja
Publication of JP2020519851A5 publication Critical patent/JP2020519851A5/ja
Priority to JP2022207680A priority Critical patent/JP2023102271A/ja
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/00002—Operational features of endoscopes
    • A61B1/00004—Operational features of endoscopes characterised by electronic signal processing
    • A61B1/00009—Operational features of endoscopes characterised by electronic signal processing of image signals during a use of endoscope
    • A61B1/000096—Operational features of endoscopes characterised by electronic signal processing of image signals during a use of endoscope using artificial intelligence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/03—Peptides having up to 20 amino acids in an undefined or only partially defined sequence; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent materials, e.g. electroluminescent or chemiluminescent
    • C09K11/06—Luminescent materials, e.g. electroluminescent or chemiluminescent containing organic luminescent materials
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent materials, e.g. electroluminescent or chemiluminescent
    • C09K11/06—Luminescent materials, e.g. electroluminescent or chemiluminescent containing organic luminescent materials
    • C09K11/07—Luminescent materials, e.g. electroluminescent or chemiluminescent containing organic luminescent materials having chemically-interreactive components, e.g. reactive chemiluminescent compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/136—Segmentation; Edge detection involving thresholding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Theoretical Computer Science (AREA)
  • Medical Informatics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Radiology & Medical Imaging (AREA)
  • Organic Chemistry (AREA)
  • Materials Engineering (AREA)
  • Molecular Biology (AREA)
  • Quality & Reliability (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Signal Processing (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Optics & Photonics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Artificial Intelligence (AREA)
JP2019553092A 2017-04-07 2018-04-05 レシオメトリック蛍光画像化方法 Pending JP2020519851A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022207680A JP2023102271A (ja) 2017-04-07 2022-12-23 レシオメトリック蛍光画像化方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762483004P 2017-04-07 2017-04-07
US62/483,004 2017-04-07
PCT/US2018/026336 WO2018187629A1 (en) 2017-04-07 2018-04-05 Ratiometric fluorescence imaging methods

Related Child Applications (1)

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JP2022207680A Division JP2023102271A (ja) 2017-04-07 2022-12-23 レシオメトリック蛍光画像化方法

Publications (2)

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JP2020519851A true JP2020519851A (ja) 2020-07-02
JP2020519851A5 JP2020519851A5 (https=) 2021-05-13

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JP2019553092A Pending JP2020519851A (ja) 2017-04-07 2018-04-05 レシオメトリック蛍光画像化方法
JP2022207680A Pending JP2023102271A (ja) 2017-04-07 2022-12-23 レシオメトリック蛍光画像化方法

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US (1) US11293868B2 (https=)
EP (2) EP3610243A4 (https=)
JP (2) JP2020519851A (https=)
KR (1) KR20190128746A (https=)
CN (1) CN110741242B (https=)
EA (1) EA201992252A1 (https=)
WO (1) WO2018187629A1 (https=)

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US20220167853A1 (en) * 2019-03-28 2022-06-02 Koite Health Oy Method for plaque detection
EP4136616B1 (en) * 2020-04-14 2024-11-06 SurgVision GmbH Verification of segmentation of luminescence images limited to analysis regions thereof
EP3907497B1 (en) * 2020-05-08 2023-08-02 Leica Microsystems CMS GmbH Apparatus and method for displaying and/or printing images of a specimen including a fluorophore
CN115697177A (zh) * 2020-07-20 2023-02-03 直观外科手术操作公司 基于图像确定发荧光物质的性质
JP2023542867A (ja) 2020-09-11 2023-10-12 グリンプス バイオ， インコーポレイテッド 疾患の検出／診断、病期分類、モニタリング及び処置のための生体外でのプロテアーゼ活性検出
WO2022056642A1 (en) * 2020-09-18 2022-03-24 Stryker European Operations Limited Systems and methods for fluorescence visualization
JP2023550687A (ja) * 2020-10-13 2023-12-05 アベラス アクイジション コーポレーション 選択的送達分子を使用する方法
CN112432934B (zh) * 2020-11-05 2021-07-06 北京中科生仪科技有限公司 发射光检测方法
WO2022147423A1 (en) * 2020-12-30 2022-07-07 Stryker Corporation Systems and methods of dual fluorophore ratiometric imaging
CN115575358B (zh) * 2021-06-21 2024-05-24 北京大学 一种对单分子荧光共振能量转移信号进行分析的方法
CN113896708B (zh) * 2021-10-29 2024-03-12 南京碳硅人工智能生物医药技术研究院有限公司 甲醛荧光探针的设计、合成及活性研究
DE102021134368B4 (de) 2021-12-22 2023-09-21 Hochschule Reutlingen Körperschaft des öffentlichen Rechts Vorrichtung und Verfahren zur Detektion von markierten Tumorzellen eines Gewebes in einer strömenden Flüssigkeit
CN114533148B (zh) * 2022-02-15 2023-06-16 佳木斯大学 一种消化内科用于胃癌检测的取样系统
EP4275590A1 (en) * 2022-05-13 2023-11-15 Leica Instruments (Singapore) Pte Ltd Data processing device and computer-implemented method combining two images and an overlay color using a uniform color space
WO2024155928A1 (en) * 2023-01-19 2024-07-25 Marquette University Systems, methods, and media for multimodal data collection and analysis for cancer screening
US20250072739A1 (en) * 2023-09-01 2025-03-06 Karl Storz Imaging, Inc. Single chip camera head for multi-spectral imaging and methods of using the same
PL448268A1 (pl) * 2024-04-11 2025-10-13 Urteste Spółka Akcyjna Związek - marker diagnostyczny guza mózgu, sposób wykrywania aktywności enzymatycznej, sposób diagnozowania guza mózgu, zestaw zawierający taki związek oraz zastosowania takiego związku i sposób leczenia guza mózgu

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JP2001017379A (ja) * 1999-07-09 2001-01-23 Fuji Photo Film Co Ltd 蛍光診断装置
JP2006189265A (ja) * 2004-12-28 2006-07-20 Kanazawa Univ アルツハイマー病の検査方法
JP2008268027A (ja) * 2007-04-20 2008-11-06 Nikon Corp 画像解析方法と、蛍光検出装置
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Publication number Publication date
KR20190128746A (ko) 2019-11-18
EP3610243A4 (en) 2020-12-16
EP3876196A1 (en) 2021-09-08
EA201992252A1 (ru) 2020-04-02
US20200150041A1 (en) 2020-05-14
CN110741242A (zh) 2020-01-31
EP3610243A1 (en) 2020-02-19
JP2023102271A (ja) 2023-07-24
WO2018187629A1 (en) 2018-10-11
US11293868B2 (en) 2022-04-05
CN110741242B (zh) 2023-07-07

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