KR20190124909A - Dressing composition for salad containing rubus coreanum vinegar - Google Patents

Dressing composition for salad containing rubus coreanum vinegar Download PDF

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KR20190124909A
KR20190124909A KR1020180048967A KR20180048967A KR20190124909A KR 20190124909 A KR20190124909 A KR 20190124909A KR 1020180048967 A KR1020180048967 A KR 1020180048967A KR 20180048967 A KR20180048967 A KR 20180048967A KR 20190124909 A KR20190124909 A KR 20190124909A
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vinegar
bokbunja
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KR102232559B1 (en
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문규환
송지영
박희전
정소희
변보영
안문규
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고창군
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/60Salad dressings; Mayonnaise; Ketchup
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12JVINEGAR; PREPARATION OR PURIFICATION THEREOF
    • C12J1/00Vinegar; Preparation or purification thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The present invention relates to a dressing composition for salad containing Rubus coreanus vinegar. The dressing composition for salad consists of purified oil, canola oil, Rubus coreanus vinegar, sugar mixtures, brewed vinegar, a Rubus coreanus juice, a seasoning and a thickener. The dressing composition for salad contains Rubus coreanus vinegar, thereby being rich in nutrients such as hydrocarbons, organic acids, vitamin and polyphenol and showing excellent antioxidant effects.

Description

복분자 식초가 함유된 샐러드용 드레싱 조성물 {DRESSING COMPOSITION FOR SALAD CONTAINING RUBUS COREANUM VINEGAR}Salad dressing composition containing bokbunja vinegar {DRESSING COMPOSITION FOR SALAD CONTAINING RUBUS COREANUM VINEGAR}

본 발명은 복분자 식초가 함유된 샐러드용 드레싱 조성물에 관한 것으로, 더욱 상세하게는 복분자 식초가 함유되어 탄수화물, 유기산, 비타민 및 폴리페놀 등과 같은 영양성분이 풍부하며, 우수한 항산화효과를 나타내는 복분자 식초가 함유된 샐러드용 드레싱 조성물에 관한 것이다.The present invention relates to a dressing composition for a salad containing bokbunja vinegar, more specifically, bokbunja vinegar is contained, rich in nutrients such as carbohydrates, organic acids, vitamins and polyphenols, containing bokbunja vinegar showing excellent antioxidant effect It relates to a dressing composition for a salad.

복분자(Rubus coreanus Miquel)는 장미과에 속하는 낙엽 활엽관목으로 높이는 2-3 m에 달하고 줄기 끝은 자주색 또는 적색이고 흰색 분말로 덮여있는 산딸기 일종이다. 5-6월에 흰색의 꽃이 피고 7-8월에 반구형의 검붉은 색 열매를 맺는 다년생 식물로 한반도 황해도 이남지방과 중국, 일본의 해발 50-1,000 m 지역 산기슭 양지에서 야생하고 있다(Yuk CS, 1990). Bokbunja (Rubus coreanus Miquel) is a deciduous broad-leaved shrub belonging to the family Rosaceae, reaching 2-3 m in height and having a purple or red stem and covered with white powder. It is a perennial plant with white flowers in May-June and hemispherical dark red fruit in July-August. It is wild in the foothills of the southern part of the Korean Peninsula and 50-1,000 m above sea level in China and Japan (Yuk CS , 1990).

또한 복분자 열매에는 탄수화물, 유기산, 비타민 B군, 비타민 C, 무기성분과 quercetin, ellagic acid, sanguiin H-5 등의 phenol성 화합물이 함유되어 있다(Lee JH et al., 2006, Pang GC et al., 1996). 복분자 열매의 항산화 활성을 지닌 Quercetin의 분리 및 동정 (Yoon et al., 2003), 복분자의 유산균 발효 및 생리활성 효과 등을 보고되고 있다(Park YS et al., 2003). 그 외에도 복분자 추출조건에 따른 페놀성 화합물의 특성 변화 (Yoon et al., 2003), 복분자의 항산화 활성(Yoon et al., 1995), 미숙 복분자로부터 가수분해된 tannin의 분리 (Lee & Lee, 1995), 미숙 복분자 분말을 첨가한 건면의 품질특성 등 다양한 생리활성이 있는 것으로 보고되고 있다(Lee et al., 2000). 복분자는 과실의 크기가 작으며 수분함량이 많아 부패하기 쉽고 저장이 어려워 복분자를 이용한 가공 식품개발에 관한 연구는 미흡한 수준이며, 기능성 식품 소재로서 활용가치가 크게 기대되고 있으나 아직 연구는 미비한 수준으로 이에 대한 활용도 잘 이루어지지 않고 있는 실정이다(Kim AJ et al., 2007b). Bokbunja fruits also contain carbohydrates, organic acids, B vitamins, vitamin C, inorganic components, and phenolic compounds such as quercetin, ellagic acid, and sanguiin H-5 (Lee JH et al., 2006, Pang GC et al. , 1996). Isolation and identification of Quercetin with antioxidant activity of bokbunja fruit (Yoon et al., 2003), lactobacillus fermentation and physiological activity of bokbunja fruit have been reported (Park YS et al., 2003). In addition, changes in the properties of phenolic compounds according to bokbunja extraction conditions (Yoon et al., 2003), antioxidant activity of bokbunja (Yoon et al., 1995), and separation of hydrolyzed tannins from immature bokbunja (Lee & Lee, 1995) ) And various physiological activities such as quality characteristics of dried noodles added with immature bokbunja powder (Lee et al., 2000). Bokbunja is small in fruit size, has high moisture content, is easy to decay, and is difficult to store, and research on processed food development using Bokbunja is insufficient, and its useful value is expected as a functional food material. It is not well utilized (Kim AJ et al., 2007b).

샐러드는 대표적인 채소의 조리방법으로 주 요리 자체로 많이 이용되며, 이에 따라 샐러드의 맛을 더해 주는 드레싱의 이용률도 증가하고 있다(Choi SN et al., 2009). 드레싱은 식사 전 식욕을 돋우어 주고 음식의 맛과 향을 증진시키며 (Kim HD et al., 2002), 색상을 부여하는 등의 역할과 육류 위주의 서구식 식단에서 영양적 균형을 맞추는 역할, 부재료 첨가로 영양가를 높이고 소화 작용을 도와주는 기능을 가지고 있다(Kim MH, 2003; Kim YH, 2007; Lee KI, 2004). Salad is widely used as a main dish as a main method of cooking vegetables, and accordingly, the utilization rate of dressing that adds the taste of salad is increasing (Choi SN et al., 2009). Dressings can stimulate appetite before meals, enhance the taste and aroma of food (Kim HD et al., 2002), add color, balance nutrition in meat-based Western diets, and add ingredients It has the function of increasing nutritional value and helping digestion (Kim MH, 2003; Kim YH, 2007; Lee KI, 2004).

샐러드를 먹기 위해서 사용되는 드레싱은 식품공전(2008)에서 “식품을 제조·가공·조리함에 있어 식품의 풍미를 돋우기 위한 목적으로 사용되는 것으로, 식용유, 식초 등을 주원료로 하여 식염, 당류, 향신료, 알류 또는 식품첨가물을 가하고 유화시키거나 분리액상으로 제조한 것 또는 이에 채소류, 과일류 등을 가한 것으로 드레싱, 마요네즈를 말한다”고 정의하고 있다. Dressings used for eating salads were used in the Food Code (2008) in order to enhance the flavor of foods in the manufacture, processing and cooking of foods, and include salt, saccharides, spices, Eggs or food additives are added and emulsified or prepared in separate liquid form, or vegetables, fruits, etc., are added to dressing and mayonnaise. ”

일반적으로 드레싱이란 대개 차가운 종류의 소스로 주로 차가운 채소에 뿌려 먹는 것을 말하며 드레싱의 종류로는 기름과 식초의 혼합 드레싱, 마요네즈가 들어간 드레싱, 조리된 드레싱의 3종류가 있고 사용하는 재료의 질에 따라 그 풍미와 물성이 좌우 된다 (Sharon, 1990). In general, dressing is a cold type of sauce, usually sprinkled with cold vegetables. There are three types of dressings: mixed dressings with oil and vinegar, dressings with mayonnaise, and cooked dressings. Its flavor and physical properties depend (Sharon, 1990).

드레싱은 주재료로 기름 및 산이 이용되고 맛과 유화작용을 돕는 향신료와 과일 등이 부재료로 혼입되기도 하는데, 이때 향신료 과일 등은 드레싱의 항산화와 항균성을 증가시키는 것으로 보고되고 있다(Frutos, M.J. et al., 2005). Dressing is mainly used as oil and acid as a main ingredient, and spices and fruits that help taste and emulsification are mixed as subsidiary materials. Spice fruits, etc., have been reported to increase the antioxidant and antimicrobial properties of dressings (Frutos, MJ et al. , 2005).

최근 건강에 대한 관심이 증가하면서 기존의 드레싱에 다양한 천연소재를 첨가한 연구가 보고되고 있으며, 이에 관한 연구로는 표고버섯 샐러드 드레싱 (Jung HA et al., 2011), 오미자 샐러드 드레싱 (Yim SB et al., 2012), 블루베리즙 요구르트드레싱 (Lee WG et al., 2012), 생강 샐러드 드레싱 (Jung HA et al., 2013), 복분자즙 요구르트드레싱 (Park JY et al., 2013), 오디즙 요구르트 드레싱 (Park KB, 2014), 머루 분말 샐러드 드레싱 (Bing DJ et al., 2015), 아로니아즙 요구르트 드레싱 (Park KB et al., 2015) 등이 보고된바 있다.Recently, as interest in health has increased, studies on adding various natural materials to existing dressings have been reported. These studies include shiitake salad dressing (Jung HA et al., 2011) and Schisandra salad dressing (Yim SB et. al., 2012), blueberry juice yogurt dressing (Lee WG et al., 2012), ginger salad dressing (Jung HA et al., 2013), bokbunja juice yogurt dressing (Park JY et al., 2013), mulberry juice Yogurt dressings (Park KB, 2014), Maroon powdered salad dressings (Bing DJ et al., 2015), Aronia juice yogurt dressings (Park KB et al., 2015) and the like have been reported.

따라서 본 연구 사업에서는 기능성이 입증된 복분자를 이용한 식초를 제조한 후 이를 이용한 샐러드용 드레싱 조성물을 개발하여 이화학적 특성 및 기능성 성분을 분석하여 기능성이 부합된 복분자 식초가 함유된 샐러드용 드레싱 조성물을 개발하고자 한다.Therefore, in this research project, after preparing vinegar using bokbunja, which proved to be functional, we developed salad dressing compositions using the same, and analyzed the physicochemical properties and functional ingredients to develop salad dressing compositions containing bokbunja vinegar with functional functionality. I would like to.

한국특허등록 제10-1125509호(2012.03.05)Korea Patent Registration No. 10-1125509 (2012.03.05) 한국특허등록 제10-1428692호(2014.08.04)Korean Patent Registration No. 10-1428692 (2014.08.04)

본 발명의 목적은 복분자 식초가 함유되어 탄수화물, 유기산, 비타민 및 폴리페놀 등과 같은 영양성분이 풍부하며, 우수한 항산화효과를 나타내는 복분자 식초가 함유된 샐러드용 드레싱 조성물을 제공하는 것이다.An object of the present invention is to provide a dressing composition for salads containing bokbunja vinegar is rich in nutrients, such as carbohydrates, organic acids, vitamins and polyphenols, containing the bokbunja vinegar, showing excellent antioxidant effect.

본 발명의 목적은 정제수, 카놀라유, 복분자 식초, 당혼합물, 양조식초, 복분자 즙, 조미료 및 증점제로 이루어지는 것을 특징으로 하는 복분자 식초가 함유된 샐러드용 드레싱 조성물을 제공함에 의해 달성된다.An object of the present invention is achieved by providing a dressing composition for salads containing bokbunja vinegar, characterized in consisting of purified water, canola oil, bokbunja vinegar, sugar mixture, vinegar, bokbunja juice, seasonings and thickeners.

본 발명의 바람직한 특징에 따르면, 상기 복분자식초를 이용한 샐러드용 드레싱 조성물은 정제수 100 중량부, 카놀라유 60 내지 70 중량부, 복분자 식초 30 내지 35 중량부, 당혼합물 60 내지 65 중량부, 양조식초 30 내지 35 중량부, 복분자 즙 10 내지 15 중량부, 조미료 20 내지 25 중량부 및 증점제 0.1 내지 0.5 중량부로 이루어지는 것으로 한다.According to a preferred feature of the invention, the salad dressing composition using the bokbunja vinegar is 100 parts by weight of purified water, 60 to 70 parts by weight of canola oil, 30 to 35 parts by weight of bokbunja vinegar, 60 to 65 parts by weight of sugar mixture, brewing vinegar 30 to It shall consist of 35 weight part, 10-15 weight part of bokbunja juice, 20-25 weight part of seasonings, and 0.1-0.5 weight part of a thickener.

본 발명의 더 바람직한 특징에 따르면, 상기 복분자 식초는 복분자와 설탕을 혼합하여 당도가 23 내지 25 brix가 되도록 혼합물을 제조한 후에, 상기 혼합물 100 중량부에 효모 0.5 내지 1.5 중량부를 투입하고, 20 내지 30℃에서 8 내지 12일간 1차 발효한 후에, 고형분을 제거하고 효모 0.05 내지 0.15 중량부를 추가로 투입하고 24 내지 26℃에서 28 내지 32일간 2차 발효하여 제조되는 것으로 한다.According to a more preferable feature of the present invention, the bokbunja vinegar is mixed with bokbunja and sugar to prepare a mixture so that the sugar content is 23 to 25 brix, 0.5 to 1.5 parts by weight of yeast is added to 100 parts by weight of the mixture, 20 to After primary fermentation at 30 ° C. for 8 to 12 days, the solids are removed, and 0.05 to 0.15 parts by weight of yeast is further added, followed by secondary fermentation at 24 to 26 ° C. for 28 to 32 days.

본 발명의 더욱 바람직한 특징에 따르면, 상기 당혼합물은 설탕과 올리고당이 1:0.7 내지 0.8 중량부로 혼합되어 이루어지는 것으로 한다.According to a more preferred feature of the invention, the sugar mixture is to be made of sugar and oligosaccharides 1: 1: 0.7 to 0.8 parts by weight of the mixture.

본 발명의 더욱 더 바람직한 특징에 따르면, 상기 조미료는 겨자씨, 소금, 건조마늘 및 후추로 이루어지는 것으로 한다.According to a further preferred feature of the invention, the seasoning is to be made of mustard seed, salt, dried garlic and pepper.

본 발명의 더욱 더 바람직한 특징에 따르면, 상기 증점제는 잔탄검으로 이루어지는 것으로 한다.According to a further preferred feature of the present invention, the thickener is composed of xanthan gum.

본 발명에 따른 복분자 식초가 함유된 샐러드용 드레싱 조성물은 복분자 식초가 함유되어 탄수화물, 유기산, 비타민 및 폴리페놀 등과 같은 영양성분이 풍부하며, 우수한 항산화효과를 나타내는 샐러드용 드레싱 조성물을 제공한다.Salad dressing composition containing bokbunja vinegar according to the present invention is rich in nutrients such as carbohydrates, organic acids, vitamins and polyphenols, containing bokbunja vinegar, and provides a salad dressing composition showing excellent antioxidant effect.

도 1은 본 발명에 실시예 1을 통해 제조된 복분자 식초가 함유된 샐러드용 드레싱 조성물을 촬영하여 나타낸 사진이다.
도 2는 본 발명의 실시예 1을 통해 제조된 복분자 식초가 함유된 샐러드용 드레싱 조성물을 보관용기에 투입한 상태를 촬영하여 나타낸 사진이다.
1 is a photograph showing a salad dressing composition containing bokbunja vinegar prepared in Example 1 in the present invention.
Figure 2 is a photograph showing a state in which the dressing composition for salad containing bokbunja vinegar prepared in Example 1 of the present invention was put in a storage container.

이하에는, 본 발명의 바람직한 실시예와 각 성분의 물성을 상세하게 설명하되, 이는 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 발명을 용이하게 실시할 수 있을 정도로 상세하게 설명하기 위한 것이지, 이로 인해 본 발명의 기술적인 사상 및 범주가 한정되는 것을 의미하지는 않는다.In the following, preferred embodiments of the present invention and the physical properties of each component will be described in detail, which is intended to explain in detail enough to be able to easily carry out the invention by one of ordinary skill in the art, This does not mean that the technical spirit and scope of the present invention is limited.

본 발명에 따른 복분자 식초가 함유된 샐러드용 드레싱 조성물은 정제수, 카놀라유, 복분자 식초, 당혼합물, 양조식초, 복분자 즙, 조미료 및 증점제로 이루어지며, 정제수 100 중량부, 카놀라유 60 내지 70 중량부, 복분자 식초 30 내지 35 중량부, 당혼합물 60 내지 65 중량부, 양조식초 30 내지 35 중량부, 복분자 즙 10 내지 15 중량부, 조미료 20 내지 25 중량부 및 증점제 0.1 내지 0.5 중량부로 이루어지는 것이 바람직하다.Salad dressing composition containing bokbunja vinegar according to the present invention is composed of purified water, canola oil, bokbunja vinegar, sugar mixture, brewed vinegar, bokbunja juice, seasonings and thickeners, 100 parts by weight of purified water, 60 to 70 parts by weight of canola oil, bokbunja It is preferably composed of 30 to 35 parts by weight of vinegar, 60 to 65 parts by weight of sugar mixture, 30 to 35 parts by weight of brewed vinegar, 10 to 15 parts by weight of bokbunja juice, 20 to 25 parts by weight of seasonings and 0.1 to 0.5 parts by weight of thickener.

상기 복분자 식초는 복분자와 설탕을 혼합하여 당도가 23 내지 25 brix가 되도록 혼합물을 제조한 후에, 상기 혼합물 100 중량부에 효모 0.5 내지 1.5 중량부를 투입하고, 20 내지 30℃에서 8 내지 12일간 1차 발효한 후에, 고형분을 제거하고 효모 0.05 내지 0.15 중량부를 추가로 투입하고 24 내지 26℃에서 28 내지 32일간 2차 발효하여 제조된다.The bokbunja vinegar is prepared by mixing the bokbunja and sugar to prepare a mixture so that the sugar content is 23 to 25 brix, 0.5 to 1.5 parts by weight of yeast is added to 100 parts by weight of the mixture, the primary at 20 to 30 ℃ for 8 to 12 days After the fermentation, the solids are removed, and 0.05 to 0.15 parts by weight of yeast is additionally added, followed by secondary fermentation at 24 to 26 ° C. for 28 to 32 days.

또한, 상기 당혼합물은 설탕과 올리고당이 1:0.7 내지 0.8 중량부로 혼합되어 이루어진다.In addition, the sugar mixture is a mixture of sugar and oligosaccharide 1: 0.7 to 0.8 parts by weight.

또한, 상기 조미료는 겨자씨, 소금, 건조마늘 및 후추로 이루어지며, 겨자씨 12 내지 14 중량부, 소금 4.5 내지 5.5 중량부, 건조마늘 3 내지 3.5 중량부 및 후추 1 내지 2 중량부로 이루어진다.In addition, the seasoning is made of mustard seed, salt, dried garlic and pepper, mustard seed 12 to 14 parts by weight, salt 4.5 to 5.5 parts by weight, dried garlic 3 to 3.5 parts by weight and pepper 1 to 2 parts by weight.

또한, 상기 증점제는 잔탄검으로 이루어지는 것이 바람직하다.Moreover, it is preferable that the said thickener consists of xanthan gum.

상기의 성분으로 이루어지는 복분자 식초가 함유된 샐러드용 드레싱 조성물은 정제수 100 중량부, 카놀라유 60 내지 70 중량부, 복분자 식초 30 내지 35 중량부, 당혼합물 60 내지 65 중량부, 복분자 즙 10 내지 15 중량부, 조미료 20 내지 25 중량부 및 증점제 0.1 내지 0.5 중량부를 균질기에 투입하고 교반하여 균질화 한 후에 균질화된 혼합물을 살균기로 이상하고 100℃의 온도에서 50 내지 70초 동안 살균하여 살균처리한 후에 보관용기에 투입하여 보관하게 된다.Salad dressing composition containing bokbunja vinegar comprising the above components is 100 parts by weight of purified water, 60 to 70 parts by weight of canola oil, 30 to 35 parts by weight of bokbunja vinegar, 60 to 65 parts by weight of sugar mixture, 10 to 15 parts by weight of bokbunja juice 20 to 25 parts by weight of seasonings and 0.1 to 0.5 parts by weight of thickener are added to a homogenizer and stirred to homogenize, and then the homogenized mixture is sterilized by sterilization at a temperature of 100 ° C. for 50 to 70 seconds and then sterilized. It will be put in and stored.

본 발명에서 사용되는 복분자의 일반성분을 분석하였고, 비교군으로 오디를 사용하여 함께 분석하여 아래 표 1에 나타내었다.The general components of bokbunja used in the present invention were analyzed and analyzed together with Audi as a comparison group, and are shown in Table 1 below.

아래 표 1에 나타낸 바와 같이 복분자와 오디의 수분함량은 86.35±1.43과 88.90±0.20로 측정되었고, 단백질은 1.34±0.01과 0.64±0.22로 오디에 비해 복분자가 약 2배 더 높게 측정되었다. 지질은 0.10±0.01과 0.42±0.51로 각각 측정되었는데 오디가 복분자에 비해 약 4배 더 높게 측정되었다. 회분은 0.63±0.05와 0.53±0.01 로 각각 확인되었다.As shown in Table 1 below, the moisture content of Bokbunja and Audi was measured to be 86.35 ± 1.43 and 88.90 ± 0.20, and the protein was 1.34 ± 0.01 and 0.64 ± 0.22. Lipids were measured to be 0.10 ± 0.01 and 0.42 ± 0.51, respectively, with Audi measuring about four times higher than Bokbunja. Ash content was found to be 0.63 ± 0.05 and 0.53 ± 0.01, respectively.

<표 1>TABLE 1

Figure pat00001
Figure pat00001

또한, 본분자의 DPPH-free radical 소거활성 측정하였는데, DPPH는 아스코르빈산 및 토코페롤, polyhydroxy 방향족 화합물, 방향족 아민류에 의해 전자나 수소를 받아 불가역적으로 안정한 분자를 형성하여 환원됨에 따라 짙은 자색이 탈색되는 원리를 이용하여 다양한 천연소재로부터 항산화 물질을 탐색하기 위해 많이 이용되고 있으며 인체 내에서 특히 지방질의 산화과정에서 생성되는 free radical들은 세포의 노화를 촉진시키고 생체세포의 방어기전을 저하시킬 뿐만 아니라 세포활성을 저해하므로 이들 free radical의 생성을 근본적으로 억제하거나 이미 생성된 free radical들의 안정화를 통해 생체세포를 보호할 필요가 있다. 전자공여작용은 이러한 생물활성 free radical에 전자를 공여하여 세포 성분의 산화를 억제시킨다. 복분자와 비교하기 위하여 고창에서 많이 재배되고 있는 오디와 함께 DPPH(2,2-diphenyl-1-picryl-hydrazyl) radical 소거활성을 측정하였고 그 결과 값은 아래 표 2에 나타내었다.In addition, DPPH-free radical scavenging activity of the main molecule was measured. DPPH is discolored with dark purple as it is irreversibly formed by reducing electrons and hydrogen by ascorbic acid, tocopherol, polyhydroxy aromatic compounds, and aromatic amines. It is widely used to search for antioxidants from various natural materials by using the principle. Free radicals generated in the oxidation process of lipids in the human body, in particular, promote the aging of cells and lower the defense mechanisms of living cells, as well as cell activities. Therefore, it is necessary to fundamentally inhibit the production of these free radicals or to protect living cells through the stabilization of free radicals already produced. The electron donating action provides electrons to these bioactive free radicals to inhibit the oxidation of cellular components. DPPH (2,2-diphenyl-1-picryl-hydrazyl) radical scavenging activity was measured together with mulberry grown in Gochang to compare with bokbunja. The results are shown in Table 2 below.

<표 2>TABLE 2

Figure pat00002
Figure pat00002

위에 표 2에 나타낸 것처럼, 두 베리의 DPPH-free radical 소거활성의 IC50 값은 각각 6.19와 5.59로 복분자가 오디에 비해 약간 높은 값을 나타내었다.As shown in Table 2 above, the IC 50 values of DPPH-free radical scavenging activity of the two berries were 6.19 and 5.59, respectively, indicating that Bokbunja was slightly higher than Audi.

또한, 본 발명에 사용되는 복분자와 오디의 ABTS radical 소거활성을 측정한 결과는 아래 표 3에 나타내었다.In addition, the results of measuring the ABTS radical scavenging activity of bokbunja and Audi used in the present invention are shown in Table 3 below.

ABTS[2,2'-azinbis-(3-ethyl-benzothiazoline-6-sulfonic acid)]는 비교적 안정한 free radical로서 DPPH 방법과 함께 항산화활성을 스크리닝하는데 많이 이용되고 있다. 또한 lipophilic 또는 hydrophilic 항산화 물질의 측정에 적용 가능한 방법으로 알려져 있다. ABTS는 양이온 라디칼을DPPH는 음이온 라디칼을 소거하는 활성을 흡광도로 측정하는 방법으로 두 방법에 대한 기질과 반응물질과의 결합정도가 달라 추출물을 이용한 라디칼 소거활성 측정값에서 차이가 나타날 수 있다ABTS [2,2'-azinbis- (3-ethyl-benzothiazoline-6-sulfonic acid)] is a relatively stable free radical and is widely used for screening antioxidant activity with DPPH method. It is also known to be applicable to the measurement of lipophilic or hydrophilic antioxidants. ABTS is a measure of the activity of scavenging cationic radicals and DPPH scavenging anion radicals by absorbance.

<표 3>TABLE 3

Figure pat00003
Figure pat00003

위에 표 3에 나타낸 것처럼, 두 베리의 ABTs radical 소거활성의 IC50 값은 각각 11.36과 16.13으로 오디가 복분자에 비해 높은 값을 나타내었다. As shown in Table 3 above, the IC 50 values of ABTs radical scavenging activity of the two berries were 11.36 and 16.13, respectively, indicating that Audi was higher than Bokbunja.

또한, 본 발명에 사용되는 복분자의 총 플라보노이드 성분을 분석하였는데, 폴라보노이드는 주로 anthocyanidins, flavonols, flavones, cathechins 및 flavanones 등으로 구성되어 있으며, 식물에 다량 존재하는 플라보노이드는 항산화 작용, 순환기 질환 예방, 항염, 항알러지, 항균, 항바이러스, 면역증강 등 다양한 기능성 생리활성 효과를 보인다고 보고하였다(Kawaguchi K 1997, Middleton E 1997, Nakagawa M 1974). 복분자와 오디의 총 플라보노이드 함량은 아래 표 4에 나타내었다.In addition, the total flavonoid components of the bokbunja used in the present invention was analyzed, which is mainly composed of anthocyanidins, flavonols, flavones, cathechins and flavanones, flavonoids present in large quantities in plants are antioxidant activity, prevent circulatory disease, It has been reported to have various functional physiological effects such as anti-inflammatory, anti-allergic, antibacterial, anti-viral, and immune enhancing (Kawaguchi K 1997, Middleton E 1997, Nakagawa M 1974). The total flavonoid content of Bokbunja and Audi is shown in Table 4 below.

<표 4>TABLE 4

Figure pat00004
Figure pat00004

위에 표 4에 나타낸 것처럼, 복분자의 총 플라보노이드 함량은 0.62mg/mL 이었고 오디는 0.59mg/mL을 나타내었다. As shown in Table 4 above, the total flavonoid content of Bokbunja was 0.62 mg / mL and the Audi showed 0.59 mg / mL.

또한, 본 발명에 사용되는 복분자의 총 폴리페놀 함량을 측정하여 아래 표 5에 나타내었다.In addition, the total polyphenol content of the bokbunja used in the present invention was measured and shown in Table 5 below.

식물체에 존재하는 페놀화합물은 2차 대사산물로서 분자 내에 하나 이상의 phenolic hydroxyl(OH)기를 가지고 있어서 단백질 및 거대분자들과 결합하는 성질이 있고 공명 안정화된 구조로써 전자를 수용하는 기작으로 항산화 반응에 직접적으로 기여하면서 다양한 식물계에서 발견되는 중요한 성분이다. 2가 금속이온과의 결합력이 우수하며 수소 공여 작용을 통해 free radical을 제거함으로써 콜레스테롤 저하, 고혈압이나 동맥경화 억제, 중성지질의 생성 억제에 의한 비만 방지 및 모세혈관의 저항? 증진 등의 효과가 있으며 항산화 작용과 항암 등의 효과를 나타내는 것으로 알려져 있다.Phenolic compounds present in plants are secondary metabolites that have one or more phenolic hydroxyl (OH) groups in their molecules, which bind to proteins and macromolecules, and are electron-resonant mechanisms that are electron-resonant and stable. It is an important ingredient found in various plant systems. Excellent binding ability with divalent metal ions and hydrogen free donation to remove free radicals to reduce cholesterol, inhibit hypertension or arteriosclerosis, prevent obesity by inhibiting the formation of neutral lipids and resist capillaries? It is known to have effects such as enhancement and to show effects such as antioxidant activity and anticancer.

<표 5>TABLE 5

Figure pat00005
Figure pat00005

이하에서는, 본 발명에 따른 복분자 식초가 함유된 샐러드용 드레싱 조성물의 제조방법 및 그 제조방법을 통해 제조된 샐러드용 드레싱 조성물의 물성을 실시예를 들어 설명하기로 한다.Hereinafter, the physical properties of the salad dressing composition containing the bokbunja vinegar according to the present invention, and the physical properties of the salad dressing composition prepared through the manufacturing method will be described.

<제조예 1><Manufacture example 1>

복분자와 설탕을 혼합하여 당도가 24 brix가 되도록 혼합물을 제조한 후에, 상기 혼합물 100 중량부에 효모 1.0 중량부를 투입하고, 5℃에서 10일간 1차 발효한 후에, 고형분을 제거하고 효모 0.1 중량부를 추가로 투입하고 25℃에서 30일간 2차 발효하여 복분자 식초를 제조하였다.After mixing the bokbunja and sugar to prepare a mixture so that the sugar content is 24 brix, 1.0 parts by weight of yeast was added to 100 parts by weight of the mixture, and after the first fermentation at 5 ℃ for 10 days, the solids were removed and 0.1 parts by weight of yeast Further added and secondary fermentation at 25 ℃ 30 days to prepare a bokbunja vinegar.

본 발명에서 원재료의 일반성분은 AOAC(2000)방법으로 분석하였다. 수분함량은 105℃ 오븐을 이용한 상압가열법을 이용하여 측정하였고, 조지질은 에테르를 용매로 사용한 속실렛법을 이용하여 속실렛장치(Extraction Unit E-816)를 이용하여 측정하였다. 단백질함량은 미량 켈달법, 회분은 550℃ 회화로를 이용한 직접 회화법으로 측정하였다.General components of the raw materials in the present invention were analyzed by the AOAC (2000) method. Moisture content was measured by an atmospheric pressure heating method using an oven at 105 ℃, crude lipid was measured using an Soxhlet apparatus (Extraction Unit E-816) using an Soxhlet method using ether as a solvent. Protein content was measured by trace Kjeldahl method and ash by direct incineration method using a 550 ° C incinerator.

또한, DPPH(2,2-diphenyl-1-picryl-hydrazyl) radical 소거활성은 농도별로 조제한 시료액 100mL에 ethanol 200mL를 가하고 2×10-4M DPPH용액 300mL를 가한 후 5초간 vortex mixer로 혼합하여 실온에서 30분간 반응시키고 ELISA(Synergy HT, Biotec, Washington DC, USA)를 사용하여 517nm에서 흡광도를 측정하였음. 대조구는 시료 대신 ethanol을 첨가하여 실험하였다.In addition, DPPH (2,2-diphenyl-1-picryl-hydrazyl) radical scavenging activity was added to 100 mL of the sample solution prepared by concentration, ethanol 200 mL was added, 300 mL of 2 × 10 -4 M DPPH solution was added, and mixed with a vortex mixer for 5 seconds. The reaction was carried out at room temperature for 30 minutes and the absorbance was measured at 517 nm using ELISA (Synergy HT, Biotec, Washington DC, USA). The control group was tested by adding ethanol instead of the sample.

DPPH radical scavenging activity(%)=(1 - 시료의 흡광도/대조구의 흡광도) ×100DPPH radical scavenging activity (%) = (1-absorbance of sample / absorbance of control) × 100

또한, ABTS radical을 이용한 항산화 활성의 측정은 potassium persulfate와의 반응에 의해 생성된 ABTS radical이 추출물 내의 항산화 물질에 의해 제거되어 radical 특유의 색인 청록색이 탈색되는 것을 이용한 방법으로 DPPH 방법과 함께 항산화 활성을 탐색하는데 자주 이용되고 있가. ABTS assay는 Art 등의 방법을 변형하여 측정하였다. 농도별 (1, 5, 10, 25, 50/mL)로 제조한 시료를 96well plate에 5mL를 취하고 pH 7.0으로 맞춘 2.45 mM ABTs 용액 195 mL를 넣은 후 잘 교반한 후 7 min동안 방치 한 다음 ELISA를 이용하여 734 nm에서 흡광도를 측정하였고, 대조구는 시료 대신 ethanol을 첨가하여 실험하였다.In addition, the measurement of antioxidant activity using ABTS radical was carried out by the ABTS radical generated by the reaction with potassium persulfate by the antioxidant in the extract to remove the index-specific blue-green color. Is it often used to ABTS assay was measured by modifying the method of Art. Take 5 mL of the sample prepared by concentration (1, 5, 10, 25, 50 / mL) into a 96well plate, add 195 mL of a 2.45 mM ABTs solution adjusted to pH 7.0, stir well, and leave for 7 min. Absorbance was measured at 734 nm by using, and the control was tested by adding ethanol instead of the sample.

ABTS radical scavenging activity (%) = (1 - A/B) × 100ABTS radical scavenging activity (%) = (1-A / B) × 100

A: Absorbance of sampleA: Absorbance of sample

B: Absorbance of blankB: Absorbance of blank

또한, 플라보노이드 함량 또한 건강기능식품공전 방법을 이용하여 측정하였다. 즉 각각의 시료 0.5 mL에 ethanol 1.5 mL, 10% 질산알루미늄 0.1 mL, 1 M 초산칼슘 0.1 mL, 증류수 2.8 mL을 순서대로 가하고 vortex mixer로 혼합하여 암소에서 40분간 반응시키고 UV/VIS spectrophotometer(UV-2450, Shimadzu Co., Kyoto, Japan)를 사용하여 415nm에서 흡광도를 측정하였다. 이때 quercetin(Sigma-Aldrich Co., St. Louis, Mo, USA)을 표준물질로 사용하여 검량곡선을 작성하고 이로부터 총 폴리페놀 함량을 구하였다. In addition, the flavonoid content was also measured using the nutraceutical method. That is, 0.5 mL of each sample was added 1.5 mL of ethanol, 0.1 mL of 10% aluminum nitrate, 0.1 mL of 1 M calcium acetate, and 2.8 mL of distilled water, and mixed with a vortex mixer for 40 minutes in the dark. The UV / VIS spectrophotometer (UV- 2450, Shimadzu Co., Kyoto, Japan) was used to measure the absorbance at 415 nm. At this time, a calibration curve was prepared using quercetin (Sigma-Aldrich Co., St. Louis, Mo, USA) as a standard material, and the total polyphenol content was determined from the calibration curve.

또한, 총 폴리페놀 함량은 페놀성 물질인 phosphomolybdic acid와 반응하여 청색을 나타내는 원리를 이용한 Folin-Denis 방법을 이용하여 측정하였다. 시료액 1mL에 Folin-Ciocalteau’s phenol regent 1mL를 가하고 3 분간 반응시킨 다음 10% Na2CO3 1mL를 넣고 1 시간 동안 실온에서 반응시킨 후 UV/VIS spectrophotometer(UV-2450, Shimadzu Co., Kyoto, Japan)를 사용하여 700nm에서 비색정량 하였다. 이때 gallic acid(Sigma-Aldrich Co., St. Louis, Mo, USA)를 표준물질로 사용하여 검량곡선을 작성하고 이로부터 총 폴리페놀 함량을 구하였다.In addition, the total polyphenol content was measured by using the Folin-Denis method using the principle of blue to react with the phenolic phosphomolybdic acid. 1 mL of Folin-Ciocalteau's Phenolic Regent was added to 1 mL of the sample solution, followed by reaction for 3 minutes, 1 mL of 10% Na 2 CO 3 , and reaction at room temperature for 1 hour, followed by UV / VIS spectrophotometer (UV-2450, Shimadzu Co., Kyoto, Japan ) Was colorimetrically determined at 700 nm. At this time, the calibration curve was prepared using gallic acid (Sigma-Aldrich Co., St. Louis, Mo, USA) as a standard and the total polyphenol content was calculated from this.

또한, 당도는 휴대용 당도계(Refractometer, PAL-1, Brix 0 to 53%, Atago, Japan)를 이용하여 시료 착즙액을 3회 반복 측정한 후 그 평균값으로 나타내었다.In addition, the sugar content was expressed as the average value after measuring the sample juice three times using a portable sugar meter (Refractometer, PAL-1, Brix 0 to 53%, Atago, Japan).

또한, pH 측정은 pH meter(SevenEasy, Mettler Toledo, Swiss)를 이용하여 3회 반복측정하였고, 산도는 자동산도계(Easy pH, METTLER TOLEDO, Swizerland)를 이용하여 측정하고 3번 반복 측정하여 평균값으로 나타내었다.In addition, pH measurement was repeated three times using a pH meter (SevenEasy, Mettler Toledo, Swiss), acidity was measured using an automatic pH meter (Easy pH, METTLER TOLEDO, Swizerland) and repeated three times to measure the average value Indicated.

또한, 색도 측정은 분광색차계(CM-5, Minolta, Osaka, Japan)를 사용하여 L(Lightness), a(Redness), b(Yellowness) 값으로 표시하였으며, 각 시료를 3회 반복 측정하여 그 평균값을 나타내었다. In addition, chromaticity measurement was expressed by L (Lightness), a (Redness), b (Yellowness) values using a spectrophotometer (CM-5, Minolta, Osaka, Japan), and each sample was repeatedly measured three times. The mean value is shown.

또한, 아미노산 분석은 HPLC를 이용하여 분석하였으며, Borate buffer, OPA/MPA, FMOC 시약을 시료와 단계적으로 혼합한 후 반응이 완료되면 시료를 칼럼에 주입하여 분석하였음. HPLC 조건은 Dionex Ultimate 3000 (Thremo Dionex, USA)로 분석하였고, 칼럼은 VDSpher 100 C18-E (4.6mm x 150mm, 3.5um/VDS optilab, Germany)을 사용하였다.In addition, the amino acid analysis was analyzed using HPLC, and after mixing the borate buffer, OPA / MPA, FMOC step by step with the sample and the reaction was injected into the column was analyzed. HPLC conditions were analyzed by Dionex Ultimate 3000 (Thremo Dionex, USA) and the column was VDSpher 100 C18-E (4.6mm x 150mm, 3.5um / VDS optilab, Germany).

또한, 무기성분은 Osborne & Voogt (1981)의 방법에 따라 시료를 질산과 과산화수소, 증류수를 이용하여 마이크로웨이브 분해기(Ultrawave, Milestone, Italy)로 시료를 분해하였고, 냉각 후 소량의 증류수로 희석한 후 50 mL 정용플라스크로 옮겨 서 증류수로 정용하였다. 이 용액을 ICP-OES(Agilent 720-ES, Melbourne, Australia)로 분석하여 무기성분의 함량을 구하였다. 분석조건은 power의 경우 1.20 kw, analysis pump rate는 15 rpm, rinse time은 10 sec로 하였다.In addition, the inorganic component was decomposed into a microwave digester (Ultrawave, Milestone, Italy) using nitric acid, hydrogen peroxide and distilled water according to the method of Osborne & Voogt (1981), after cooling and diluted with a small amount of distilled water The flask was transferred to a 50 mL flask and distilled with distilled water. This solution was analyzed by ICP-OES (Agilent 720-ES, Melbourne, Australia) to determine the content of inorganic components. The analysis conditions were 1.20 kw for power, 15 rpm for analysis pump rate, and 10 sec for rinse time.

또한, 유기산 분석은 시료를 호모게나이저로 고르게 균질화한 후 10 g을 100 mL 용량 플라스크에 취한 후 증류수로 표시선까지 정확히 채우고, 초음파로 1시간 추출하였음. 5℃의 원심분리기 3,000 rpm에서 30분간 원심분리한 후 상등액을 취한 다음, membrane filter (0.45 μm)로 여과 후 HPLC (Dionex Ultimate 3000 (USA)로 분석하였으며, 표준 유기산의 각 retention time을 확인하였고, 그 때의 표준 유기산 면적과 시료의 면적으로부터 함량을 계산하였다. In addition, the organic acid analysis was homogenized the sample evenly with a homogenizer, and then 10 g was taken in a 100 mL volumetric flask and accurately filled with distilled water to the indicator line, and extracted by ultrasonic for 1 hour. After centrifugation at 3,000 rpm for 5 minutes at 3,000 rpm, the supernatant was collected, filtered through membrane filter (0.45 μm), analyzed by HPLC (Dionex Ultimate 3000 (USA)), and each retention time of the standard organic acid was confirmed. The content was calculated from the area of the standard organic acid and the area of the sample at that time.

또한, 유리당 분석은 시료 5 g에 증류수 50 mL를 정용하여 30℃ 수욕조에서 30분간 진탕한 후 15,000 rpm에서 20분간 원심분리 한 다음 25 mL로 정용하고 0.45 μm syring filter로 여과시킨 것을 HPLC ystems (Waters 2695, Milford, CT, USA)로 분석하였음. HPLC 조건으로 칼럼은 carbohydrate analysis (4.6×150 mm, TST, Waters, Newcastle, DE, USA)를 사용하였고, 용매는 acetonitrile-water (75:25v/v), 유속은 1.5 mL/min으로 분석하였고, 유리당 함량은 시료 중의 각 유리당과 동일한 표준물질 (Sigma Chemical Co., St. Louis, MO, USA)을 이용하여 작성한 검량선으로부터 계산하였다.In addition, free sugar analysis was performed by shaking 50 mL of distilled water to 30 g in a 30 ° C. water bath, centrifuging for 20 min at 15,000 rpm, then 25 mL and filtering with 0.45 μm syring filter. Waters 2695, Milford, CT, USA). HPLC analysis was performed using carbohydrate analysis (4.6 × 150 mm, TST, Waters, Newcastle, DE, USA), acetonitrile-water (75: 25v / v), and a flow rate of 1.5 mL / min. Free sugar content was calculated from calibration curves prepared using the same standard (Sigma Chemical Co., St. Louis, Mo., USA) as each free sugar in the sample.

상기 제조예 1을 통해 제조된 복분자 식초의 당도, 산도, pH를 측정하여 아래 표 6에 나타내었다.The sugar, acidity, and pH of the bokbunja vinegar prepared through Preparation Example 1 were measured and shown in Table 6 below.

<표 6>TABLE 6

Figure pat00006
Figure pat00006

위에 표 6에 나타낸 것처럼, 복분자 식초의 당도는 10°Brix 였고 산도와 pH는 각각 5.05 %와 3.32를 나타냈다.As shown in Table 6 above, the sugar content of Bokbunja vinegar was 10 ° Brix, and the acidity and pH were 5.05% and 3.32, respectively.

또한, 상기 제조예 1을 통해 제조된 복분자 식초의 색도를 측정하여 아래 표 7에 나타내었다.In addition, the chromaticity of the bokbunja vinegar prepared in Preparation Example 1 was measured and shown in Table 7 below.

<표 7>TABLE 7

Figure pat00007
Figure pat00007

위에 표 7에 나타낸 것처럼, 복분자 식초의 명도 값을 나타내는 L 값은 24.49±0.09를 나타냈고 적색도인 a 값은 43.61±0.02를 나타냈으며 황색도 값인 b 값은 41.58±0.16을 나타내었다.As shown in Table 7 above, the L value representing the brightness value of Bokbunja vinegar was 24.49 ± 0.09, the redness a value was 43.61 ± 0.02, and the yellowness value b value was 41.58 ± 0.16.

또한, 상기 제조예 1을 통해 제조된 복분자 식초의 아미노산을 분석하여 아래 표 8에 나타내었다.In addition, the amino acids of the bokbunja vinegar prepared in Preparation Example 1 are shown in Table 8 below.

<표 8>TABLE 8

Figure pat00008
Figure pat00008

위에 표 8에 나타낸 것처럼, 아미노산 중 proline이 58.71 mg/L로 가장 높은 값을 나타내었고, 그 다음은 alanine으로 56.94 mg/L 순이었으며, histidine이 1.97 mg/L로 가장 낮은 값을 나타내었다.As shown in Table 8 above, proline among the amino acids showed the highest value with 58.71 mg / L, followed by alanine with 56.94 mg / L, followed by histidine with the lowest value of 1.97 mg / L.

또한, 상기 제조예 1을 통해 제조된 복분자 식초의 무기성분을 분석하여 아래 표 9에 나타내었다.In addition, the inorganic components of the bokbunja vinegar prepared in Preparation Example 1 are shown in Table 9 below.

<표 9>TABLE 9

Figure pat00009
Figure pat00009

위에 표 9에 나타낸 것처럼, 복분자 식초의 무기성분은 K가 1351.453 mg/L로 가장 높은 함량을 나타냈으며 Mg이 126.416 mg/L, Ca가 107.547 mg/L 순으로 높게 나타났다.As shown in Table 9, the inorganic components of bokbunja vinegar showed the highest content of 1351.453 mg / L K, followed by Mg 126.416 mg / L, Ca 107.547 mg / L in order.

또한, 상기 제조예 1을 통해 제조된 복분자 식초의 유기산 성분을 분석하여 아래 표 10에 나타내었다.In addition, the organic acid component of the bokbunja vinegar prepared in Preparation Example 1 is shown in Table 10 below.

<표 10>TABLE 10

Figure pat00010
Figure pat00010

위에 표 10에 나타낸 것처럼, 복분자 식초의 주요 유기산은 Acetic acid이었으며 그 함량은 38931.442 mg/L로 나타났다. 이어서 Citric acid, Lactic acid로 각각 1905.739 mg/L, 854.529 mg/L 로 높았으며 Malic acid가 231.095 mg/L로 가장 낮은 값을 나타냈다.As shown in Table 10 above, the main organic acid of bokbunja vinegar was Acetic acid and its content was 38931.442 mg / L. Citric acid and Lactic acid were 1905.739 mg / L and 854.529 mg / L, respectively, and Malic acid was the lowest at 231.095 mg / L.

또한, 상기 제조예 1을 통해 제조된 복분자 식초의 유리당 성분을 분석하여 아래 표 11에 나타내었다.In addition, the free sugar component of the bokbunja vinegar prepared in Preparation Example 1 is shown in Table 11 below.

<표 11>TABLE 11

Figure pat00011
Figure pat00011

위에 표 11에 나타낸 것처럼, 복분자 식초의 주요 유리당 성분은 fructose 와 glucose로 나타났으며, 그 함량은 각각 7879.217와 6506.876mg/L이었다.As shown in Table 11 above, the major free sugar components of bokbunja vinegar were fructose and glucose, and their contents were 7879.217 and 6506.876 mg / L, respectively.

<실시예 1><Example 1>

정제수 30g, 카놀라유 20g, 상기 제조예 1을 통해 제조된 복분자식초 10g, 설탕 11g, 올리고당 8, 양조식초 10g, 복분자 즙 4g, 겨자씨 3.9g, 소금 1.5g, 건조마늘 1g, 후추 0.5g, 잔탄검 0.1g을 균질기에 투입하고 30분 동안 균질화한 후에, 균질화된 혼합물을 살균기에 투입하고 100℃의 온도에서 60초 동안 살균하여 복분자 식초가 함유된 샐러드용 드레싱 조성물을 제조하였다.Purified water 30g, canola oil 20g, bokbunja vinegar 10g, sugar 11g, oligosaccharide 8, brewed vinegar 10g, mustard seed 4g, mustard seed 3.9g, salt 1.5g, dried garlic 1g, pepper 0.5g, xanthan gum After adding 0.1 g to the homogenizer and homogenizing for 30 minutes, the homogenized mixture was added to the sterilizer and sterilized for 60 seconds at a temperature of 100 ° C. to prepare a dressing composition for a salad containing bokbunja vinegar.

상기 실시예 1을 통해 제조된 복분자 식초가 함유된 샐러드용 드레싱 조성물의 당도, 산도, pH를 측정하여 아래 표 12에 나타내었다.The sugar, acidity, and pH of the salad dressing composition containing bokbunja vinegar prepared through Example 1 were measured and shown in Table 12 below.

단, 당도는 휴대용 당도계(Refractometer, PAL-1, Brix 0 to 53%, Atago, Japan)를 이용하여 시료 착즙액을 3회 반복 측정한 후 그 평균값으로 나타내었다.However, the sugar content was expressed as the average value after measuring the sample juice three times using a portable sugar meter (Refractometer, PAL-1, Brix 0 to 53%, Atago, Japan).

또한, 산도는 자동산도계(Easy pH, METTLER TOLEDO, Swizerland)를 이용하여 측정하고 3번 반복 측정하여 평균값으로 나타내었다.In addition, the acidity was measured by using an automatic acidimeter (Easy pH, METTLER TOLEDO, Swizerland) and repeated three times to represent the average value.

또한, pH 측정은 pH meter(SevenEasy, Mettler Toledo, Swiss)를 이용하여 3회 반복측정하였다. In addition, pH measurement was repeated three times using a pH meter (SevenEasy, Mettler Toledo, Swiss).

<표 12>TABLE 12

Figure pat00012
Figure pat00012

또한, 상기 실시예 1을 통해 제조된 복분자 식초가 함유된 샐러드용 드레싱 조성물의 점도를 측정하여 아래 표 13에 나타내었다.In addition, the viscosity of the salad dressing composition containing bokbunja vinegar prepared in Example 1 was measured and shown in Table 13 below.

단, 점도는 Viscometer (brookfield, LVDV Ⅱ, USA) 이용하여 100 rpm으로 spindle LV2룰 사용하여 상온에서 측정하였으며 spindel이 돌기 시작한 후 1분 정도 되는 순간의 점도를 cP(centi poise)단위로 읽었다. However, the viscosity was measured at room temperature using a spindle LV2 at 100 rpm using a Viscometer (brookfield, LVDV II, USA), and the viscosity at about 1 minute after the spindel began to spin was read in units of cP (centi poise).

<표 13>TABLE 13

Figure pat00013
Figure pat00013

또한, 상기 실시예 1을 통해 제조된 복분자 식초가 함유된 샐러드용 드레싱 조성물의 색도를 측정하여 아래 표 14에 나타내었다.In addition, the chromaticity of the salad dressing composition containing bokbunja vinegar prepared in Example 1 was measured and shown in Table 14 below.

단, 색도는 분광색차계(CM-5, Minolta, Osaka, Japan)를 사용하여 L(Lightness), a(Redness), b(Yellowness) 값으로 표시하였으며, 각 시료를 3회 반복 측정하여 그 평균값을 나타내었다. However, chromaticity was expressed as L (Lightness), a (Redness), and b (Yellowness) using a spectrophotometer (CM-5, Minolta, Osaka, Japan), and the average value of each sample was measured three times. Indicated.

<표 14>TABLE 14

Figure pat00014
Figure pat00014

또한, 상기 실시예 1을 통해 제조된 복분자 식초가 함유된 샐러드용 드레싱 조성물의 영양성분을 분석하여 아래 표 15에 나타내었다.In addition, the nutritional composition of the salad dressing composition containing bokbunja vinegar prepared in Example 1 is shown in Table 15 below.

단, 영양성분 분석은 ㈜ 세스코 시험분석센터에 의뢰하여 분석하였다. 분석 내용으로는 9대 영양성분인 나트륨, 당류, 열량, 조단백질, 조지방, 콜레스테롤, 탄수화물, 트랜스지방 및 포화지방 항목을 분석하였다.However, nutritional component analysis was commissioned by Sesco Corporation. As for the analysis, the nine major nutrients such as sodium, sugar, calories, crude protein, crude fat, cholesterol, carbohydrate, trans fat and saturated fat were analyzed.

<표 15>TABLE 15

Figure pat00015
Figure pat00015

위에 표 15에 나타낸 것처럼, 본 발명의 실시예 1을 통해 제조된 복분자 식초가 함유된 샐러드용 드레싱 조성물은 1회 제공량 100ml로 탄수화물은 영양소 기준치 7%, 당류는 18%, 단백질은 1%, 지방은 41%, 포화지방은 7% 및 나트륨은 36%를 나타냈다.As shown in Table 15 above, the salad dressing composition containing bokbunja vinegar prepared in Example 1 of the present invention in a single serving amount of 100ml carbohydrates 7% nutrients, sugars 18%, protein 1%, fat Silver 41%, saturated fat 7% and sodium 36%.

본 발명에 따른 복분자 식초가 함유된 샐러드용 드레싱 조성물은 복분자 식초가 함유되어 탄수화물, 유기산, 비타민 및 폴리페놀 등과 같은 영양성분이 풍부하며, 우수한 항산화효과를 나타낸다.Dressing composition for salads containing bokbunja vinegar according to the present invention is rich in nutrients such as carbohydrates, organic acids, vitamins and polyphenols containing bokbunja vinegar, and shows an excellent antioxidant effect.

Claims (6)

정제수, 카놀라유, 복분자 식초, 당혼합물, 양조식초, 복분자 즙, 조미료 및 증점제로 이루어지는 것을 특징으로 하는 복분자 식초가 함유된 샐러드용 드레싱 조성물.
Dressing composition for salads containing bokbunja vinegar, characterized in that consisting of purified water, canola oil, bokbunja vinegar, sugar mixture, brewed vinegar, bokbunja juice, seasonings and thickeners.
청구항 1에 있어서,
상기 복분자식초를 이용한 샐러드용 드레싱 조성물은 정제수 100 중량부, 카놀라유 60 내지 70 중량부, 복분자 식초 30 내지 35 중량부, 당혼합물 60 내지 65 중량부, 양조식초 30 내지 35 중량부, 복분자 즙 10 내지 15 중량부, 조미료 20 내지 25 중량부 및 증점제 0.1 내지 0.5 중량부로 이루어지는 것을 특징으로 하는 복분자 식초가 함유된 샐러드용 드레싱 조성물.
The method according to claim 1,
The salad dressing composition using the bokbunja vinegar is 100 parts by weight of purified water, 60 to 70 parts by weight of canola oil, 30 to 35 parts by weight of bokbunja vinegar, 60 to 65 parts by weight of sugar mixture, 30 to 35 parts by weight of brewed vinegar, bokbunja juice 10 to Dressing composition for salads containing bokbunja vinegar, characterized in that consisting of 15 parts by weight, seasoning 20 to 25 parts by weight and thickener 0.1 to 0.5 parts by weight.
청구항 1 또는 2에 있어서,
상기 복분자 식초는 복분자와 설탕을 혼합하여 당도가 23 내지 25 brix가 되도록 혼합물을 제조한 후에, 상기 혼합물 100 중량부에 효모 0.5 내지 1.5 중량부를 투입하고, 20 내지 30℃에서 8 내지 12일간 1차 발효한 후에, 고형분을 제거하고 효모 0.05 내지 0.15 중량부를 추가로 투입하고 24 내지 26℃에서 28 내지 32일간 2차 발효하여 제조되는 것을 특징으로 하는 복분자 식초가 함유된 샐러드용 드레싱 조성물.
The method according to claim 1 or 2,
The bokbunja vinegar is prepared by mixing the bokbunja and sugar to prepare a mixture so that the sugar content is 23 to 25 brix, 0.5 to 1.5 parts by weight of yeast is added to 100 parts by weight of the mixture, the primary at 20 to 30 ℃ for 8 to 12 days After fermentation, the dressing composition for salads containing bokbunja vinegar, characterized in that the solid content is removed, and added to 0.05 to 0.15 parts by weight of yeast and secondary fermentation at 24 to 26 ℃ for 28 to 32 days.
청구항 1 또는 2에 있어서,
상기 당혼합물은 설탕과 올리고당이 1:0.7 내지 0.8 중량부로 혼합되어 이루어지는 것을 특징으로 하는 복분자 식초가 함유된 샐러드용 드레싱 조성물.
The method according to claim 1 or 2,
The sugar mixture is a dressing composition for salad containing bokbunja vinegar, characterized in that the sugar and oligosaccharide is 1: 0.7 to 0.8 parts by weight.
청구항 1 또는 2에 있어서,
상기 조미료는 겨자씨, 소금, 건조마늘 및 후추로 이루어지는 것을 특징으로 하는 복분자 식초가 함유된 샐러드용 드레싱 조성물.
The method according to claim 1 or 2,
The seasoning is salad dressing composition containing bokbunja vinegar, characterized in that consisting of mustard seeds, salt, dried garlic and pepper.
청구항 1 또는 2에 있어서,
상기 증점제는 잔탄검으로 이루어지는 것을 특징으로 하는 복분자 식초가 함유된 샐러드용 드레싱 조성물.
The method according to claim 1 or 2,
The thickening agent salad dressing composition containing bokbunja vinegar, characterized in that consisting of xanthan gum.
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KR20230092334A (en) * 2021-12-17 2023-06-26 주식회사 씨드밀 Salad dressing containing natural ingredients and Manufacturing method for the same

Citations (5)

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Publication number Priority date Publication date Assignee Title
KR100206521B1 (en) * 1991-08-07 1999-07-01 이중덕 Dressing
KR101125509B1 (en) 2009-12-23 2012-03-21 상주시 Mulberry sauce and preparation method thereof
KR20120061447A (en) * 2010-12-03 2012-06-13 농업회사법인(주)농가사랑 Manufacturing Method for Multi-purpose Sauce Using Rubus coreanus Skin and Unpolished rice Skin
KR101428692B1 (en) 2013-01-18 2014-08-11 전북대학교산학협력단 Fermented Organic Rubus Coreanus Vinegar With Antioxidative Function And Process Of Preparing The Same
KR101583447B1 (en) * 2015-06-09 2016-01-19 홍성표 Dressing sauce for meat with high palatability and functionality and preparation method thereof

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KR100206521B1 (en) * 1991-08-07 1999-07-01 이중덕 Dressing
KR101125509B1 (en) 2009-12-23 2012-03-21 상주시 Mulberry sauce and preparation method thereof
KR20120061447A (en) * 2010-12-03 2012-06-13 농업회사법인(주)농가사랑 Manufacturing Method for Multi-purpose Sauce Using Rubus coreanus Skin and Unpolished rice Skin
KR101428692B1 (en) 2013-01-18 2014-08-11 전북대학교산학협력단 Fermented Organic Rubus Coreanus Vinegar With Antioxidative Function And Process Of Preparing The Same
KR101583447B1 (en) * 2015-06-09 2016-01-19 홍성표 Dressing sauce for meat with high palatability and functionality and preparation method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230092334A (en) * 2021-12-17 2023-06-26 주식회사 씨드밀 Salad dressing containing natural ingredients and Manufacturing method for the same

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