KR20190117989A - Composition comprising insect of Bombus sp. extract for protecting aged internal organ - Google Patents

Abstract

The present invention relates to a composition for protecting aged body organs, containing an extract of Bombus sp. insects, wherein a pharmaceutical composition or a health functional food containing the extract has excellent cell proliferation and anti-inflammatory effects of aged pancreas, kidney or spleen, thereby being able to be easily used as an anti-aging composition for protecting various aged body organs.

Description

Composition for protection of aging body organs, characterized in that it contains an extract of insects of the genus Backyoung bee {Composition comprising insect of Bombus sp. extract for protecting aged internal organ}

The present invention relates to a composition for protecting the aged body organs, characterized in that it contains an extract of insects of the genus Beetle.

In today's fast-paced aging society, delaying the aging process is one of the key health challenges. In Korea, the population aged 65 or older accounted for 11.3% in 2010, and is expected to increase to 37.3% in 2050. In such an aging society, the increase in disease morbidity and enormous medical expenses are expected, and the proliferation of senile diseases such as dementia is expected to be a social problem.

The process of aging is influenced by a combination of genetic, environmental, and lifestyle interactions, and is accompanied by a variety of morphological and biochemical changes. In particular, increased oxidative stress and increased inflammatory response are involved in aging. It is considered to be the main cause. Oxidative stress can be caused by increased production of reactive oxygen species (ROS) or by the reduction of antioxidant defense mechanisms in the body. As a result, biopolymers such as DNA are destroyed and cell damage can promote aging and dementia. Including neurodegenerative diseases, cancer, cardiovascular diseases, such as to increase the risk of aging diseases.

On the other hand, aging is a phenomenon in which normal somatic cells can no longer divide after a certain number of divisions, which contributes to aging of individuals and tissues, and is an important mechanism for suppressing abnormal proliferation of cells in the body organs and cancer formation. Aged cells increase in shape, flatten, increase heterochromatin in the nucleus, increase fear in the cytoplasm, increase SA-β-gal (senescence-associated β-galatosidase) activity, Increasing amounts of proteins that inhibit cell growth such as p53, p16INK4, and p21, insulin-like growth factor binding proteins (IGFBPs), interleukin-6, and conversion growth factor- It secretes various inflammatory proteins such as β (transforming growth factor-β; TGF-β) and interferon. Adler et al. (Adler AS, Sinha S, Kawahara TL, Zhang JY, Segal E, Chang HY.Motif module map reveals enforcement of aging by continual NF-kappaB activity.Genes Dev. 2007; 21: 3244-57) It has been shown that the inflammatory response and the aging process are closely related to tissue and experimental animal tissue. Aging of cells in body organs not only contributes to the aging of an individual or tissue, but also plays an important role in the pathogenesis of various diseases. Aged cells are frequently found in inflammatory lesion tissues such as rheumatoid arthritis, osteoarthritis, hepatitis, chronic skin damage tissues, and atherosclerotic vascular tissues. In addition, cell aging is observed in prostate hyperplasia, hepatitis, liver cancer and the like.

As senescent cells accumulate in the organs of the body, senescent cells do not divide well, and thus damaged tissues are not properly repaired, and they secrete enzymes or inflammatory cytokines that break down surrounding tissues, thereby accelerating tissue damage. Contribute to the etiology of diseases associated with.

The present inventors, while studying the various physiological activities of the insects in the back bee, the extract of the insects in the back bee affects the proliferation of cells extracted from the aged organs and inhibits the production of inflammatory factors It was completed.

Republic of Korea Patent Application Publication No. 10-2011-0013074 (Name of the invention: new uses and manufacturing method of insect glycozyminoglycans, Applicant: Republic of Korea, published date: 2011.02.09) Republic of Korea Patent Publication No. 10-2012-0075214 (Invention name: Method for preparing insect-derived heparin replacement formulation composition and its use, Applicant: Republic of Korea, published date: 2012.07.06 Republic of Korea Registered Patent No. 10-1680644 (Name of the invention: N-glycan derived from the queen bee and its use, Applicant: Korea, Registered Date: 2016.11.23) Republic of Korea Patent Registration No. 10-1491765 (Invention: Composition for the prevention and treatment of diseases caused by angiogenesis, including the extract of the alcohol extract of the back young bee, Applicant: Republic of Korea, registered date: 2015.02.03)

It is an object of the present invention to provide an aging body organ protective composition, characterized in that it contains an extract of insects of the genus Be Young.

The present invention relates to a composition for protecting the aged body organs, characterized in that it contains an extract of insects of the genus Beetle.

The bumblebee insect may be selected from the group consisting of Western common carder bee (Bombus terrestris), bees (Bombus ignitus), common carder bee (Bombus hypocrita sapporoensis Cockerell) and some common carder bee (Bombus ardens) to sappo.

The aged body organs may be pancreas, kidney or spleen.

The composition is characterized in that it has the effect of inhibiting cell proliferation or inflammation of the aged pancreas, kidney or spleen. The inhibitory effect of inflammation can be mediated by inhibiting IL-10.

The present invention can provide a health functional food for protecting the aged body organs, characterized in that it contains an extract of insects in the genus beetle.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

Insects of the genus beetle can be used one or more selected from larva, pupa, worker bee adult, queen bee adult, cane adult and cocoon.

The extract of the insects of the genus beetle can extract the insects of the genus beetle with water, C1 ~ C4 alcohol, acetone or a mixture thereof as a solvent, the C1 ~ C4 alcohol is methanol, ethanol, propanol, isopropanol, butanol and iso It may be selected from the group consisting of butanol. Among the solvents in the present invention, most preferably ethanol or acetone may be used.

Water, C1 ~ C4 alcohol, acetone or a mixture solution thereof used in the preparation of the extract of the insect insects of the back young bees may be used 1 to 40 times the volume (1 to 40 L based on 1 kg) based on the weight of insects used in the back young bees, preferably 5 to 40 times the volume can be used. Extraction conditions of the extract of the insect in the back young bees may be 1 minute to 48 hours at 20 ~ 100 ℃. The process may be repeated 1 to 4 times.

The present invention also provides a pharmaceutical composition for protecting the aged body organs containing the extract of insects of the genus bee. The extract of the insects of the genus Youngbeul may be added as 0.001 to 100% by weight to the pharmaceutical composition of the present invention. The pharmaceutical compositions may be used in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral formulations, external preparations, suppositories, and sterile injectable solutions, respectively, according to a conventional method. Carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , Methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid form preparations include at least one excipient such as starch, calcium carbonate, sucrose or lactose, It is prepared by mixing gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The dosage of the pharmaceutical composition of the present invention will vary depending on the age, sex, weight of the subject to be treated, the specific disease or pathology to be treated, the severity of the disease or pathology, the route of administration and the judgment of the prescriber. Dosage determination based on these factors is within the level of skill in the art and generally dosages range from 0.01 mg / kg / day to approximately 2000 mg / kg / day. More preferred dosage is 1 mg / kg / day to 500 mg / kg / day. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, livestock, humans, etc. by various routes. All modes of administration can be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or cerebrovascular injections. Since the extract of the present invention has little toxicity and side effects, it is a drug that can be used safely even when taken for long periods of time.

In another aspect, the present invention provides a dietary supplement for protecting the aged body organs, including the extract of insects in the genus beetle and food acceptable food supplement additives. The extract of the insect insects of the genus Youngbeul may be added as 0.001 to 100% by weight to the health functional food of the present invention. The health functional food of the present invention includes the form of tablets, capsules, pills, or liquids, and the food to which the extract of the present invention can be added includes, for example, various drinks, meats, sausages, breads, candy, Snacks, noodles, ice cream, dairy products, soups, ionic drinks, beverages, alcoholic beverages, gum, tea and vitamin complexes.

The present invention relates to a composition for protecting the aged body organs, characterized in that it contains an extract of the insect Bombu s sp., Wherein the pharmaceutical composition or health functional food containing the extract is aged pancreas, kidney or spleen. It can be easily used as an anti-aging composition for protecting various aging body organs due to its excellent cell proliferation and anti-inflammatory effects.

1 is a result confirming the cell proliferation effect of the insect extract in the back young bees in the spleen cells collected from the aged mice.
Figure 2 is a result confirming the cell proliferation effect of the insect extract in the back young bees in the kidney cells collected from the aged mice.
Figure 3 is a result confirming the cell proliferation effect of the insect extract in the back young bees in the pancreas cells collected from the aged mice.
Figure 4a is a result confirming the inhibitory effect of IL-10 possessed by the insect extract of the back bee in the spleen cells collected from the aged mice.
Figure 4b is a result confirming the inhibitory effect of IL-10 possessed by the insect extract in the backing bee on kidney cells collected from aged mice.
Figure 5 is a result confirming the inhibitory effect of IL-10 possessed by the insect extract in the backing bee in the pancreas cells collected from the aged mice.

Hereinafter, a preferred embodiment of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided to fully convey the spirit of the present invention to those skilled in the art so that the contents introduced herein are thoroughly and completely.

Example 1 Preparation of Insect Beetle Insect Sample

Table 1 below shows the insect samples used in the present invention. Insects of each backing bee were insects obtained by breeding in the National Academy of Agricultural Sciences. (Remarks-Insect No. 11 was taken from Taebaek and then reared). In order to prepare the extract, each insect shown in Table 1 was pulverized into a living body, soaked in acetone or each 75% (v / v) ethanol aqueous solution, and immersed at low temperature (about 4 ° C), and treated with ultrasonic waves three times at an interval of 1 hour per day. After immersion (total 3 days), the resultant was filtered and concentrated under reduced pressure and freeze-dried to obtain an extract (dried).

sample Abbreviation Condition of Insects Used as Raw Material Samples Extract Type One BTLP Western common carder bee larvae in which changes in one of the pupae (Bombus terrestris) Acetone extract 2 BIW Worker Bees from Bumblebee ( Bombus ignitus ) Acetone extract 3 Btcocn Cocoon without the larvae and pupa of Bombus terrestris worker bees Acetone extract 4 ZomBTQ Adult queen bee of Bombus ardens ardens Acetone extract 5 BIcocn Cocoon removed from larvae and pupa of bumblebee ( Bombus ignitus ) worker Acetone extract 6 SabW Adult worker bees of Bombus hypocrita sapporoensis Acetone extract 7 BIQ Adult queen bee of bumblebee ( Bombus ignitus ) Acetone extract 8 BTPu Chrysalis changing into an adult of a worker bee ( Bombus terrestris pupa) Acetone extract 9 BIL Worker bee larvae of bumblebee ( Bombus ignitus ) Acetone extract 10 BIP Bumblebee ( Bombus ignitus ) pupa during its transformation into adult Acetone extract 11 TabasabQ Queen Bee Adult of Bombus hypocrita sapporoensis Acetone extract 12 ZomBtalcolex Adult queen bee of Bombus ardens ardens 75% (v / v) ethanol
Aqueous solution extract 13 BTQ Adult queen bee of Bombus terrestris Acetone extract 14 ㅅㄷㅇ Western bee queen bee
(1/10 dilution of sample 13) Acetone extract

** cocoon: cocoon / bongon. Egg form of thin membrane. Unlike silkworms, which stay in Cocoon only during the pupa stage, insects in the rearing bees grow in individual cocoons from the larval stage to the pupa stage.

** Sapporo Back Young Bee: Also known as Sapporo Back Young Bee.

<Example 2. Confirmation of cell proliferation effect>

The spleen, kidney and pancreas of 18 months old aging rats (869.5 grams in weight, SD rats) were collected and isolated from each other.

To explain the cell collection process in more detail, senile mice were removed from the spleen, kidney, and pancreas in sterilized state, and each organ cell was separated by tweezers in a 20ml DMEM (Gibco) medium and scraped with a 23 gauge needle. Then transfer each suspension of the spleen, pancreas, and kidney cells into a 50 ml conical tube, allow to stand for 5 minutes at room temperature to allow the cell debris to settle, and then remove the supernatant from 50 ml cinical tibe containing 5 ml fetal bovine serum (Sigma). After pipetting so as not to mix the two layers in the centrifugation at 500 g for 5 minutes at room temperature, the cell precipitate was suspended in 20 ml of medium. After that, the cell suspension was added to 0.17M Tris-NH ₄ Cl solution warmed to 37 ° C. in order to destroy red blood cells, and left for 5 minutes. Then, pipetting the two layers into a 20 ml conical tube containing 5 ml fetal bovine serum, washing the cells three times with DMEM medium, and controlling the cells by measuring the number of cells using a hemocytometer. Aliquots were made and media volume was adjusted to 180 μl. Each extract of Table 1 was prepared by diluting each extract of Table 1 in a medium at a concentration of 50 mg / ml and 5 mg / ml in 96 wells, and adding 20 μl to the medium so that the total volume of the medium was 200 μl. Incubated for 2 days at mg / ml and 0.5 mg / ml.

Subsequently, the survival rate of the cells was confirmed using an XTT assay kit (Cell Proliferation kit II (XTT) Cat. No. 1 465 015 [Roche Diagnostics GmbH, Mannheim, Germany]), compared with the absorbance criteria of 490 nm-650 nm. The survival rate of tissue cells of Btcocon and BIQ was increased. 1 to 3 and Table 2, Kidney cells were significant in all groups, spleen cells and pancreas cells were also significant in most groups compared to the no treatment group (CON).

<Example 3. Confirmation of IL-10 Inhibitory Activity>

Spoons, kidneys, and pancreas of 18-month-old aging rats (869.5 grams in weight) made by self-cultivation were isolated from each cell, and 200 μl of the 96 wells were dispensed into each well of 50 mg / ml. It was prepared by diluting the medium at a concentration of 5 mg / ml, and adding 20 μl to the medium so that the total volume of the medium was 200 μl and incubating for 2 days. The substrate was then analyzed after confirmation from 450-540 nm using an IL-10 activity to ^{ELISA kit (R & D Quantikine ®} ElISA Rat IL-10).

The results are shown in Table 3 and FIG. 4 and FIG. 5, which showed that the amount of IL-10 increased in aging (inflammatory) as a whole decreased by the sample.

Formulation Example 1 Pharmaceutical Formulation-Preparation of Tablet

200 g of the extract of oleracea was mixed with 175.9 g of lactose, 180 g of potato starch, and 32 g of colloidal silicic acid. 10% gelatin solution was added to the mixture, which was then ground and passed through a 14 mesh sieve. It was dried and the mixture obtained by adding 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate was made into a tablet.

Preparation Example 2 Food Preparation

Formulation Example 2-1. Preparation of Cooking Seasonings

The extract of the adult adult bee bee was added as 1% by weight to the cooking seasoning was prepared for health promotion cooking seasoning.

Formulation Example 2-2. Manufacture of Flour Food

The extract of the kkaeyoung bee was added to the flour in 0.1% by weight, and the mixture was used to prepare bread, cake, cookies, crackers and noodles to prepare foods for health promotion.

Formulation Example 2-3. Preparation of soups and gravy

The extract of the bumblebee was added to the soup and gravy 0.1% by weight to prepare a soup and gravy for health promotion.

Formulation Example 2-4. Manufacture of dairy products

Extracts of bumblebee larvae were added to the milk at 0.1% by weight, and the milk was used to prepare various dairy products such as butter and ice cream.

Formulation Example 2-5. Vegetable Juice Manufacturing

0.5 g of the queen bee extract was added to 1,000 ml of tomato juice or carrot juice to prepare vegetable juice for health promotion.

Formulation Example 2-6. Fruit juice manufacturing

0.1 g of the extract of the larvae of the Western young bees was added to 1,000 ml of apple juice or wine to prepare fruit juice for health promotion.

Claims (11)

Composition for protecting the aged body organs, characterized in that it contains an extract of the insect Bombu s sp. The method of claim 1,
The insects of the genus bee beetle ( Bombus terrestris ), bumble bee ( Bombus ignitus ), Sapporo back bee (Bloomus hypocrita sapporoensis Cockerell) and the back of the bee ( Bombus ardens ) composition for protecting the aged body organs, characterized in that . The method of claim 1,
The organ is a pancreas, kidney or spleen aging body composition, characterized in that. The method of claim 1,
The composition is an organ body protective composition, characterized in that the pancreas, kidney or spleen has a cell proliferation effect. The method of claim 1,
The composition is an aging body organ protective composition, characterized in that the pancreas, kidney or spleen has an inhibitory effect on inflammation. A dietary supplement for protecting the aged organs, comprising an extract of an insect of Bombus sp. The method of claim 6,
The bumblebee insect is Western common carder bee (Bombus terrestris), bees (Bombus ignitus), common carder bee to sappo (Bombus hypocrita sapporoensis Cockerell) and some common carder bee (Bombus ardens) aging, characterized in that selected from the group consisting of organ protection health Nutraceutical. The method of claim 6,
The organ is a pancreas, kidney or spleen aging body functional health food, characterized in that. The method of claim 6,
The composition is a functional health food for protecting the aged body organs, characterized in that the pancreas, kidney or spleen has a cell proliferation effect. The method of claim 6,
The composition is a functional health food for protecting the aged body organs, characterized in that the pancreas, kidney or spleen has an inhibitory effect on inflammation. A composition for inhibiting aging of the body, characterized in that it contains an extract of insects of Bombu s sp.

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