KR20190101067A - A cosmetic composition containing fermented broussonetia kazinoki siebold and setaria italica and the method thereof - Google Patents

Abstract

The present invention relates to: a cosmetic composition containing a fermented Setaria italica and Broussonetia kazinoki mixture as an active ingredient, which is obtained by mixing an extract extracted from Setaria italica and Broussonetia kazinoki, followed by primary fermentation using fermentation microorganisms, and then secondary fermentation through a process of aging in a cedar container; and a method for producing the same.

Description

Cosmetic composition containing a mulberry and a crude fermented product as an active ingredient and a method for producing the same {A COSMETIC COMPOSITION CONTAINING FERMENTED BROUSSONETIA KAZINOKI SIEBOLD AND SETARIA ITALICA AND THE METHOD THEREOF}

The present invention relates to a cosmetic composition containing a mulberry and a crude fermentation as an active ingredient, and more particularly, to a primary fermentation by adding microorganisms to a mixture of mulberry (Broussonetia kazinoki Siebold) and crude (Setaria italica) And then, to a cosmetic composition comprising a mixed fermented extract of the mulberry and crude prepared through a two-stage fermentation process to secondary fermentation again through low temperature aging process.

The present invention relates to a mask pack cosmetic composition comprising a natural substance having collagen synthesis enhancement, skin regeneration, wrinkle improvement and antioxidant effects, and fermentation of Broussonetia kazinoki Siebold and crude (Setaria italica) as the natural substance. It relates to a cosmetic composition using water.

Functional cosmetics refers to a product that helps to improve wrinkles on the skin or a product that burns the skin finely or protects the skin from ultraviolet rays. Recently, researches on functional cosmetics of plant-derived ingredients are being actively conducted, and in particular, in the case of domestic functional cosmetics using natural materials, more active R & D is being made than other cosmetic fields.

In this direction, Korean cosmetics companies, which have entered the fierce R & D line, are bridging the technological gap with advanced cosmetics countries by leading competitive new material developments and leading the leap into high value-added industries. Of these, functional cosmetics using natural extracts are emerging as a new research tendency in the Korean cosmetics market, and the trend is attracting attention in the skin-beauty market as it combines ethnic preferences for beauty.

In accordance with the above circumstances, the present invention by applying a fermentation product obtained by fermenting the mixture of a mixture of natural mulberry and crude which is known to have a skin cosmetic effect in two steps to a conventional formulation, The present invention proposes a new cosmetic composition and a technique for producing the same, which have significantly improved antioxidant and skin regeneration effects as compared to the cosmetic composition using the bath.

On the other hand, it is recognized as a functional cosmetic material, but the main components of the mulberry sap is non-volatile components and aromatic components accounting for about 70%, the remainder is water and solids. These sapwood sap has various effects such as wrinkle improvement, collagen synthesis enhancement, skin regeneration and antioxidant effect, but there is a problem in that conventional cosmetic formulations have limitations due to insolubility characteristics.

Although the extract is known to have a skin whitening or skin wrinkle improvement effect, it has been studied that the activity of the extract as a cosmetic depends greatly on the fermentation method and the fermentation strain to obtain the extract.

Next, the prior art existing in the field to which the technology of the present invention belongs will be briefly described, and then the technical matters to be made differently from the prior art will be described.

First, Korean Patent Publication No. 10-2012-0003081 (published Jan. 10, 2012) relates to an external composition for slimming containing a mulberry extract, and more specifically, from stems, roots, leaves, flowers and fruits of the mulberry. By containing the obtained extract as an active ingredient, a technique for slimming the skin external preparation composition excellent in the subcutaneous lipolysis effect of the adipocytes and the cellulite removal effect is described.

In addition, Korean Patent Laid-Open Publication No. 10-2015-0092567 (2015.08.13.) Discloses a cosmetic for whitening and a method for manufacturing the same, and more particularly, contains a plant extract to suppress the production of melanin, thereby causing red spots. As a cosmetic for whitening which prevents and improves pigmentation of freckles and the like, a technique relating to a cosmetic preparation method manufactured using plant extract from persimmon soup, bear horsetail and cedars is described.

In addition, Korean Patent Laid-Open Publication No. 10-2012-0140527 (published Dec. 31, 2012) relates to a cosmetic composition containing a fermented crude extract, and includes Bifidobacterium sp. Or Lactobacillus sp. It describes a technique related to a cosmetic composition for skin whitening or wrinkle improvement comprising an extract of a crude (Setaria italica) fermented by the strain.

Although the prior art documents have some similarities to the present invention in that the cosmetic composition using the mulberry or jaw, the prior art documents by using the fermentation of fermentation mixture of the mulberry and jaw, as in the present invention, When used alone, it does not include the content of having a significantly superior antioxidant effect and skin regeneration effect, and moreover, the prior art documents are related to the two-stage fermentation process that can maximize the function as a cosmetic as in the present invention and It didn't contain anything to infer.

The present invention has been created to solve the above problems, while being able to apply a variety of cosmetic formulations, cosmetic composition comprising a mulberry sap fermented product that can have collagen synthesis enhancement, skin regeneration, wrinkle improvement and antioxidant effect as an active ingredient And the purpose is to provide a method of manufacturing the same.

According to an embodiment of the present invention, a method of preparing a cosmetic composition containing a mulberry and a crude fermented product as an active ingredient, distilled water and ethanol is added to each of the mulberry and the crude immersed, separated into a supernatant and a precipitate to extract the mulberry extract and the crude extract. An extraction step; A mixing step of preparing a mulberry extract and a crude extract mixture by mixing the mulberry extract and the crude extract extracted in the extraction step; A primary fermentation step of preparing primary mulberry and crude mixed fermentation by adding fermentation microorganisms to the mulberry and crude extract mixture; And a secondary fermentation step of preparing the secondary mulberry and the crude mixed fermentation by placing the primary mulberry and the crude mixed fermented product in a cedar barrel and ripening them.

In addition, as an embodiment, the extraction step, by adding 500 to 2000 parts by weight of 70% ethanol prepared by mixing distilled water and ethanol for 100 parts by weight and 100 parts by weight of the mulberry, and soaked for 6 hours at 50 ℃ It is characterized by extracting the extract.

In addition, as an embodiment, the mixing step, characterized in that the mixing ratio of the mulberry extract and the crude extract to 8: 2: 2 to 8.

In addition, as an embodiment, the primary fermentation step, Pseudozyma sp., Lactobacillus sp., Bacillus sp., Bacillus sp. Leuconostoc sp.) By inoculating and fermenting one microorganism selected from the group consisting of, characterized in that the primary mulberry and crude mixed fermentation product.

In addition, as an embodiment, the primary fermentation step, characterized in that the fermentation for 60 to 80 hours while stirring at 20 ~ 40 ℃ 100 ~ 200rpm.

In addition, as an example, the secondary fermentation step, the primary mulberry and crude mixed fermented product in the cedar barrel and filtered for 5 to 10 days fermented liquid fermented at 2 ~ 10 ℃ secondary mulberry and crude It is characterized by obtaining a mixed fermentation product.

In addition, the cosmetic composition containing a mulberry and a crude fermented product according to another embodiment of the present invention as an active ingredient, after the primary fermentation using a fermentation microorganism by mixing the extract extracted from the mulberry and the crude, and then in the cedar barrel It is characterized in that it contains a mulberry and crude mixed fermentation product obtained by secondary fermentation through aging process as an active ingredient.

In addition, as an embodiment, the extract is added to 500 to 2000 parts by weight of 70% ethanol prepared by mixing distilled water and ethanol for 100 parts by weight and 100 parts by weight of the mulberry, and then immersed for 6 hours at 50 ℃ It is characterized in that the extraction.

In addition, as an embodiment, the mixing of the extract extracted from the mulberry and the crude, characterized in that the mixing ratio of the mulberry extract and the crude extract is 8: 2: 2 to 8:

In addition, as an embodiment, the fermentation microorganism, Pseudozyma sp. (Pseudozyma sp.), Lactobacillus (Lactobacillus sp.), Bacillus (Bacillus sp) and in the group consisting of Leuconostoc sp. It is characterized in that at least one selected.

In addition, as an embodiment, the primary fermentation, characterized in that the fermentation for 60 to 80 hours while stirring at 20 ~ 40 ℃ 100 ~ 200rpm.

In addition, as an embodiment, the secondary fermentation, characterized in that the primary fermented product obtained through the primary fermentation in a cedar barrel and aged at 2 ~ 10 ℃ for 5 to 10 days.

The present invention relates to a cosmetic composition comprising a fermented product of mulberry and crude as an active ingredient, and a method for producing the same, wherein the mixed fermentation of mulberry and crude fermented through a two-stage fermentation process according to the present invention, the antioxidant effect, collagen synthesis effect And excellent skin regeneration effect, cosmetic composition containing it as an active ingredient has an anti-aging effect and wrinkle improvement effect.

1 is a flow chart showing a method for producing a cosmetic composition containing fermented products of mulberry and crude according to an embodiment of the present invention as an active ingredient.
Figure 2 is a graph showing the results of collagen biosynthesis experiments of cosmetic compositions containing the fermentation of mulberry and crude according to an embodiment of the present invention as an active ingredient.
Figure 3 is a graph showing the skin regeneration test results of the cosmetic composition containing a fermented product of mulberry and crude according to an embodiment of the present invention.

Hereinafter, with reference to the accompanying drawings, a cosmetic composition containing a mulberry and crude fermentation according to the present invention as an active ingredient so that those of ordinary skill in the art can easily carry out the present invention and its preparation Preferred embodiments of the method will be described in detail.

In describing the principles of the preferred embodiment of the present invention in detail, if it is determined that the detailed description of the related known functions or configurations may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted.

In addition, the configuration shown in the embodiments and drawings described herein are only one of the most preferred embodiments of the present invention and do not represent all of the technical spirit of the present invention, various modifications that can be replaced at the time of the present application It should be understood that there may be equivalents and variations.

In the present invention, it is possible to apply a variety of cosmetic formulations, while presenting a cosmetic composition containing the mulberry and crude fermentation as an active ingredient having collagen synthesis enhancement, skin regeneration, wrinkle improvement and antioxidant effects. More specifically, the cosmetic composition according to the present invention is prepared by inoculating microorganisms in an extract mixture of a mulberry extract and a crude extract by primary fermentation, followed by secondary fermentation through low-temperature aging in a cedar barrel, and thus prepared. The fermented product is applicable to a variety of cosmetic formulations, and will have an effect on a variety of skin care.

One of the natural materials used in the present invention is locust tree (Broussonetia kazinoki Siebold) is a deciduous broad-leaved shrub native to Korea, Taiwan, and Japan. It grows mainly in low mountain areas.子) It is effective in treating back pain or edema, and the bark of bark is described as low bark (楮 樹皮), which treats edema and swelling, drains water, and urinates well. The mulberry tree is 3m tall, the bark is gray, and the leaves are 5 ~ 20cm long, egg-shaped or long egg-shaped, serrated at the edge, and deeply dug into 2-3 pieces. It is known as the main ingredient for making traditional Korean paper rather than medicinal herbs. Recently, it has been reported that the mulberry extract has a whitening effect has been of interest.

It contains hexane, chloroform, ethylacetate, butanol, aqueous, etc., and it contains kazinol J of kazinol and luteolin of flavonoids. In addition, the phenolic components of the stems and roots of mulberry inhibit the formation of lipid peroxide, which causes aging and deactivation, and inhibit the formation of melanin, which has the effect of whitening the skin color.

Another natural material used in the present invention (Setaria italica) is a long-growing crop that has been cultivated before and after all similarities in all parts of the temperate region of Asia, and is said to have been introduced in China before rice was introduced. Among cereals, the seed is the smallest and the shelf life is strong. There are many kinds, but there are 'chajo' which is persistent when it's open and 'mezzo' which is not. Looking at the nutritional composition of the crude bath, it contains about 10% of protein, half of each of prolamin and glutelin, and less of lysine and less leucine in prolamin. There are many. Carbohydrate is about 70%, most of which is starch. The shape of starch is similar to that of rice. Other ingredients include moisture, lipids, fibrin, ash and flavonoids. In particular, the tank contains antioxidants to inhibit the oxidation of the cells to prevent skin aging has the effect of rejuvenating the organs and skin.

In addition, Cedar (Cryptomeria japonica) is an evergreen tree native to Japan and is widely distributed in Jeju and southern regions. Cedar contains various types of terpenoids, especially sesquiterpenes of 15 carbons as main components. These ingredients are known to have antifungal activity against dermatophytes on their own.

Hereinafter, with reference to the drawings it will be described in detail with respect to the cosmetic composition and manufacturing method containing the mulberry and the crude fermented product according to the present invention as an active ingredient.

1 is a flow chart showing a method for producing a cosmetic composition containing a mulberry and a crude fermented product according to an embodiment of the present invention as an active ingredient.

As shown in FIG. 1, first, distilled water and ethanol are added to each of the mulberry and the tank to be immersed to separate the supernatant and the precipitate to perform the extraction step of extracting the mulberry extract and the crude extract (S101).

 More specifically, the extraction step is added to 500 to 2000 parts by weight of 70% ethanol prepared by mixing distilled water and ethanol for 100 parts by weight and 100 parts by weight of the mulberry, and then immersed at 50 ℃ for 6 hours to extract the extract do.

In addition, the extraction step may extract the mulberry and crude extracts in a conventional extraction process known in the art. For example, a single or mixed solution of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), acetone, ethyl acetate, chloroform, butyl acetate or 1,3-butylene glycol An extract can also be obtained as an extraction solvent. On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained in addition to the above-described extraction solvent, as well as the extract of the present invention exhibiting substantially the same effects using other extraction solvents. In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process.

After performing the extraction step (S101), and performs the mixing step to prepare the mulberry and crude extract mixture by mixing the extract of the mulberry extract and the crude extract in the extraction step (S102).

In the mixing step, the mixing ratio of the mulberry extract and the crude extract is mixed at 8: 2 to 2: 8, and preferably the mixing ratio of the mulberry extract and the crude extract is 6: 4 to 4: 6. More preferably, the mulberry extract and the crude extract are mixed and used in the same ratio.

After performing the mixing step (S102), the fermentation microorganism is added to the mulberry and crude extract mixture to perform a primary fermentation step to prepare a primary mulberry and crude mixed fermentation (S103).

More specifically, the first fermentation step, after the addition of the mulberry and crude extract mixture to the prepared medium and sterilization, Pseudozyma sp. (Pseudozyma sp.), Lactobacillus (Lactobacillus sp.), Bacillus ( Bacillus sp) and Leukonstock stock (Leuconostoc sp.) Genus one fermented microorganism selected from the group consisting of inoculation.

The medium is preferably selected according to the type of the inoculated microorganism, glucose, K2HPO4, KH2PO4, NaCl, yeast extract, malt extract, peptone, olive oil, glutamic acid, DMEM (animal cell culture medium) ), It is preferable to use a medium prepared with one or a mixture thereof selected from EpiLife (Cascade fluid).

After inoculation of the fermentation microorganisms selected in the medium as described above, the fermentation is carried out for 60 to 80 hours, preferably 72 hours with stirring at 100 to 200 rpm, preferably 130 to 150 rpm at 20 to 40 ° C., preferably 25 to 35 ° C. It is preferable to proceed. As the fermentation step is performed, primary mulberry and crude mixed fermentation products having hydrophilic properties are prepared.

After performing the primary fermentation step (S103), the primary mulberry and crude mixed fermented product is put into a cedar barrel and aged to carry out the secondary fermentation step to prepare a secondary mulberry and crude mixed fermentation (S104). Secondary mulberry and crude mixed fermentations obtained through such a process are used as cosmetic compositions according to the invention.

More specifically, the secondary fermentation step is to put the primary mulberry and crude mixed fermentation in cedar barrels and filtered the liquid fermentation matured at a low temperature of 2 ~ 10 ℃ for 5-10 days by filtering the secondary mulberry and crude Obtain fermented product.

In the filtration, the liquid fermentation product is centrifuged and filtered to prepare primary mulberry and crude mixed fermentation products. As described above, the liquid fermented product is removed by removing the insoluble matter such as cells and the supernatant through centrifugation, followed by paper filtration of 0.3 to 0.5 μm, preferably 0.45 μm, followed by the sterilization and filtration of the secondary mulberry according to the present invention. Mixed fermentations (cosmetic compositions according to the invention) can be obtained.

As described above, the extraction of the anti-aging active ingredient is enhanced by undergoing the second aging process in the cedar barrel, and the secondary mixed fermentation product of the present invention is a primary mixed fermentation product which does not undergo secondary fermentation, or a general pottery jar. It was confirmed that the antioxidant and collagen production activity is superior to the secondary fermented product fermented in the secondary fermentation.

Cosmetic compositions containing the mulberry and crude fermented products prepared by performing such a process as an active ingredient can be applied to various cosmetic formulations, and have various effects such as wrinkle improvement, collagen synthesis enhancement, skin regeneration and antioxidant effect. It is possible to secure a high value as a cosmetic. For example, it may be formulated into cosmetics such as solutions, suspensions, emulsions, gels, lotions, essences, creams, powders, soaps, mask packs, cleansing, oils, foundations, and the like.

For reference, the cosmetic composition according to the present invention may further contain other ingredients within the range not impairing the effect of the present invention in addition to the above-mentioned effective ingredient.

In addition, the components included in the cosmetic composition of the present invention contains components commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, and for example, conventional auxiliaries such as stabilizers, solubilizers, vitamins, pigments and flavors, and carriers. can do.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethylcarbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, aluminum meta Hydroxide, microcrystalline cellulose, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a cream or gel, wax, paraffin, trakant, animal oil, starch, cellulose derivative, silicone, bentonite, polyethylene glycol, silica, zinc oxide or talc may be used as the carrier component.

When the formulation of the present invention is a powder or a spray, the carrier component may include a propellant such as silica, talc, aluminum hydroxy group, lactose, calcium silicate, chlorofluorohydrocarbon, propane / butane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an imidazolinium derivative, an isethionate, a methyltaurate, a sarcosinate, a fatty acid amide ether. Sulfates, aliphatic alcohols, alkylamidobetaines, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

<Example 1>

Weigh 50 g each of mulberry and jaw, add 70% ethanol to each of 10 times the weight of mulberry and jaw, and soak for 6 hours at 50 ° C. to extract the mulberry extract and the crude extract, and then mix them in the same weight ratio. A crude extract mixture was prepared.

The above-mentioned mulberry and crude extract mixtures were added to the medium (3% glucose, 1% Glutamic acid, 1% K2HPO4, 0.5% KH2PO4), and then sterilized, inoculated with Bacillus sp. Microorganisms, stirred at 140 rpm at 30 ° C, and 72 hours. Fermentation to prepare primary mulberry and crude mixed fermentation.

The primary mulberry and crude mixed fermented products were aged at low temperature at 2-8 ° C. for 7 days in a cedar barrel, and then the liquid fermented product was removed by centrifugation to remove insoluble matters such as cells, and the supernatant was recovered. After filtration and sterile filtration to prepare a secondary mulberry and crude mixed fermentation (cosmetic composition comprising the active material of the mulberry and crude fermentation according to the present invention).

Comparative Example 1

70 g of ethanol was added to 50 g of mulberry and 10 times the weight of the mulberry and the crude, and soaked at 50 ° C. for 6 hours to extract the mulberry extract.

Comparative Example 2

To 50 g of the bath, 70% ethanol was added 10 times the weight of the mulberry and the bath and soaked at 50 ° C. for 6 hours to extract the crude extract.

Experiment 1: Measurement of DPPH Free Radical Scavenging Capacity

DPPH free radical scavenging activity was measured according to the method of Nanjo et al. (1996). 30 μl of 60 μM DPPH (1,1-diphenyl-2-picrylhydrazyl) solution was mixed with 30 μl of the sample, and the reaction solution was transferred to a 100 μl quartz capillary tube for 2 minutes, followed by an electron spin resonance (ESR) spectrometer (JES-FA, JEOL). Ltd., Tokyo, Japan). Experimental conditions are as follows. (magnetic field, 336.5 ± 5 mT; power, 5 mW; modulation frequency, 9.41 GHz; amplitude, 1 × 1,000; sweep time, 30 s; temperature, 298 K.)

Experiment 1 Results

DPPH radical scavenging ability was calculated by the difference of the relative radical signal peak height between H and H0, the results are shown in Table 1 below.

DPPH  free radical scavenging activity ( % ) = (1-H / H0) × 100 Example 1 67.87 ± 0.34 Comparative Example 1 49.27 ± 1.26 Comparative Example 2 52.14 ± 0.70

As shown in Table 1, the cosmetic composition as a active ingredient in the mulberry and crude fermentation according to the present invention was shown to have excellent DPPH free radical scavenging ability compared with the mulberry extract and crude extract.

It can be seen that the cosmetic composition of the present invention extracted by fermenting the mulberry and crude through a two-stage fermentation process significantly improved anti-aging activity compared to the cosmetic composition using only the mulberry or crude extract.

Experiment 2: collagen biosynthesis promoting effect

Human dermal fibroblasts were seeded in 48-well microplates containing DMEM medium containing 10% fetal calf serum (1 × 10 ⁵ cells / well) and CO 2 at 5% concentration (CO 2) Incubator was incubated for 24 hours at 37 ℃. The serum prepared in Example 1 was added to a serum-free DMEM medium added with a final concentration of 0.1, 1, 10, 100, 500, 1000 ㎍ / ㎖ and 24 serum-free DMEM medium was not added with the mulberry sap fermentation as a control After further incubation for hours, cell cultures were harvested.

The amount of collagen produced in the collected cell culture was measured using a collagen protein measurement kit of Takara Shuzo Co., Ltd. in Japan, and the results are shown in FIG. 2.

Experiment 2 results

As shown in Figure 2, it was found that the collagen biosynthesis rate is increased under the conditions treated with the addition of the cosmetic composition according to the present invention compared to the control group without the cosmetic composition according to the present invention.

Experiment 3: Skin Regeneration Effect

Skin cell culture

Human dermal fibroblasts were purchased from Gibco (Life Technologies, Carlsbad, Calif., USA). Cells were incubated at 37 ° C. in the DMEM medium (Gibco). The medium was maintained until 80% confluence of attached cells and passage culture was performed at 80% confluence. Medium was changed every two days until confluence. The subculture was washed with a phosphate buffered saline (PBS) in which the culture medium was removed and the cells were dropped into 0.25% trypsin-EDTA (Gibco BRL, Grand Island, NY, USA), followed by centrifugation of the cell suspension. After that, the cell number was measured and subcultured three times again in the culture.

Measurement of migration of skin cells

Put the DMEM medium (Gibco Co., Ltd.) with 10% serum in a 6 well plate, and inoculate 1 × 10 ⁶ of human skin fibroblasts, and incubate at 5% CO 2 and 37 ° C. for 24 hours. Was attached to the petri dish. Each well was washed twice with serum depleted culture, and then scratched the cells by using a micro tip to induce scratch damage. Culture medium treated with a cosmetic composition of the present invention prepared in accordance with Example 1 cell layer according to each concentration (1, 10, 100, 1000 ㎍ / ㎖), a control culture medium without adding anything and the preparation method of Comparative Example 1 In the positive control culture medium added to the treatment of the extract of the young mulberry extract, the degree of scratch recovery after 24 hours incubation was confirmed by a microscope equipped with a microimage video camera (Boyertown, PA), the results are shown in FIG. Indicated.

Experiment 3 results

As shown in Figure 3, the positive control condition treated with the mulberry extract can be seen that the dermal regeneration effect is improved compared to the control group is not added. However, the conditions treated with the cosmetic composition according to the present invention were superior to HDF cell mobility at all concentrations, compared to the positive control condition added with the mulberry extract, and the dermal regeneration effect compared to the positive control condition treated with only the mulberry extract It can be seen that is improved.

As demonstrated in the results of Experiments 1 to 3, the cosmetic composition containing the mulberry and the crude fermented product according to the present invention as an active ingredient, appears to have an excellent effect on the antioxidant effect, collagen synthesis effect and skin regeneration effect In particular, the mulberry or crude extracts were found to have significantly superior antioxidant and skin regeneration effects when used alone.

The present invention has been described above with reference to the embodiments illustrated in the accompanying drawings, which are merely exemplary, and various modifications and equivalent other embodiments may be made by those skilled in the art to which the present invention pertains. Will understand. Therefore, the technical protection scope of the present invention will be defined by the claims below.

S101: Extraction Step
S102: Mixing Step
S103: first fermentation stage
S104: secondary fermentation stage

Claims (6)

An extraction step of distilling distilled water and ethanol into each of the mulberry and the bath to separate the supernatant and the precipitate to extract the mulberry extract and the crude extract;
A mixing step of preparing a mulberry extract and a crude extract mixture by mixing the mulberry extract and the crude extract extracted in the extraction step;
A primary fermentation step of preparing primary mulberry and crude mixed fermentation by adding fermentation microorganisms to the mulberry and crude extract mixture; And
Containing the primary mulberry and crude mixed fermented product in the cedar barrel and fermented by secondary fermentation step to produce a secondary mulberry and crude mixed fermentation; containing the mulberry and crude fermentation as an active ingredient Cosmetic composition manufacturing method. The method according to claim 1,
The extraction step,
For 100 parts by weight and 100 parts by weight of the mulberry, 500 to 2000 parts by weight of 70% ethanol prepared by mixing distilled water and ethanol were added and then immersed at 50 ° C. for 6 hours to extract the extract,
The mixing step,
A method of producing a cosmetic composition containing a mulberry and a crude fermented product as an active ingredient, characterized in that the mixing ratio of the mulberry extract and the crude extract is 8: 2 to 2: 8. The method according to claim 1,
The primary fermentation step,
One species of microorganism selected from the group consisting of Pseudozyma sp., Lactobacillus sp., Bacillus sp and Leuconostoc sp. By inoculating and fermenting, it is to obtain the primary mulberry and crude mixed fermentation, fermentation for 60 to 80 hours while stirring at 20 to 40 ℃ 100 to 200 rpm,
The secondary fermentation step,
Put the primary mulberry and crude mixed fermentation in cedar barrel and fermented fermented to dried mulberry and crude fermented product characterized in that the filtered fermented liquid fermented at 2 ~ 10 ℃ for 5 to 10 days Cosmetic composition manufacturing method containing as an active ingredient. It is characterized in that it contains the mulberry and the crude mixed fermentation product obtained by mixing fermented extract from the mulberry and the crude fermentation using fermentation microorganism, and then fermented in the cedar barrel secondary fermentation as an active ingredient Cosmetic composition containing a mulberry and a crude fermented product as an active ingredient. The method according to claim 4,
The extract is,
For 100 parts by weight and 100 parts by weight of the mulberry, 500 to 2000 parts by weight of 70% ethanol prepared by mixing distilled water and ethanol were added and then immersed at 50 ° C. for 6 hours to extract the extract,
Mixing the extract extracted from the mulberry and the tank,
The mixing ratio of the mulberry extract and the crude extract is 8: 2 to 2: 8,
The fermentation microorganism,
Pseudozyma sp., Lactobacillus sp., Bacillus sp., Bacillus sp. And L. Cosmetic composition containing water as an active ingredient. The method of claim 4, wherein
The primary fermentation,
It contains a mulberry and crude fermented product as an active ingredient, characterized in that the fermentation for 60 to 80 hours while stirring at 20 ~ 40 ℃ 100 ~ 200rpm,
The secondary fermentation,
Cosmetic composition containing a mulberry and crude fermentation as an active ingredient, characterized in that the primary fermented product obtained through the primary fermentation in a cedar barrel and aged at 2 ~ 10 ℃ for 5 to 10 days.

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