KR20190099867A - Composition for controlling plant powdery mildew comprising tobacco extract - Google Patents
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Abstract
Description
본 발명은 포도스파이라 푸스카(Podosphaera fusca)에 대해 높은 항균활성을 가지는 담배 추출물을 유효성분으로 포함하는 식물 흰가루병 방제용 조성물과 이의 제조방법 및 식물 흰가루병 방제제에 관한 것이다.The present invention relates to a composition for controlling plant powdery mildew comprising a tobacco extract having a high antimicrobial activity against Podosphaera fusca as an active ingredient, a preparation method thereof, and a plant powdery mildew control agent.
식물의 흰가루병(Powdery Mildew)은 물의 잎·줄기에 흰가루 형태의 반점이 생기는 식물병으로서, 시설 재배 및 노지 재배에서 많이 발생하는 병으로 난방 시설이 열악한 비닐하우스에서는 이 병의 발생이 적었으나, 최근 시설재배에서 난방 시스템의 변화로 인하여 재배지 내의 환경이 상당히 건조하게 됨에 따라 낮은 습도(60∼70 %의 상대습도)에서 흰가루병의 발병이 점차 증가하는 추세이다. Plant Mildew (Pwdery Mildew) is a plant disease that causes spots in the form of white powder on the leaves and stems of water. It is a disease that occurs frequently in plant cultivation and open field cultivation. As the environment in the plantation is considerably dry due to changes in the heating system in plant cultivation, the incidence of powdery mildew is gradually increasing at low humidity (60 to 70% relative humidity).
작물에서 흰가루병을 일으키는 병원균으로는 약 20여 종으로 알려져 있으며 Erysiphe속, Microsphaera속, Podosphaera속, Phillactinia속, Uncinula속, Sphaerotheca속에 속하는 곰팡이종(種) 들의 특수한 변종에 의하여 생기게 된다. 구체적으로는, 오이 흰가루병의 원인균인 스패로테카 풀리지니아(Sphaerotheca fuliginea),장미 흰가루병의 원인균인 스패로테카 파노사(S. pannosa), 사과나무 흰가루병의 원인균인 포도스파이라 류코트리차(Podosphaera leucotricha), 맥류 흰가루병의 원인균인 블루메리아 그라미니스(Blumeria graminis), 메론 흰가루병의 원인균인 포도스파이라 푸스카(Podosphaera fusca)등이 있다. 유성 세대를 기준으로 균을 분류하면 작물에 따라 그 속명은 다르지만, 작물의 생육기에 흰가루병의 전염원 및 발병에 주요 역할을 하는 불완전세대를 기준으로 균을 분류하면 모두 오이디움(Oidium) 속으로 분류된다. 따라서 흰가루병목에 속한 흰가루병의 원인균을 방제하기 위해서는 거의 동일한 살균제를 사용할 수 있다.There are about 20 known pathogens that cause powdery mildew in crops and are caused by special strains of fungi, which belong to the genus Erysiphe, Microsphaera, Podosphaera, Phillactinia, Uncinula, and Sphaerotheca. Specifically, Sphaerotheca fuliginea, a causative agent of cucumber powdery mildew, S. pannosa, a causative agent of rose powdery mildew, Posphaera leucotricha, a causative agent of apple tree powdery mildew, Blumeria graminis, a causative agent of powdery mildew, and Podosphaera fusca, a causative agent of melon powdery mildew, are included. If you classify bacteria based on sexual generation, the genus is different according to crops, but if you classify bacteria based on incomplete generation, which plays a major role in the epidemic and pathogenesis of powdery mildew, all of them are classified as Oidium. . Therefore, in order to control the causative agent of powdery mildew belonging to the powdery mildew bottle, almost the same fungicide can be used.
이들 병원균은 자낭각 또는 균사의 형태로 병든 식물체의 잔재에서 월동하여 1차전염원이 되며, 시설재배에서는 분생포자가 공기전염이 되어 계속해서 발생한다. 밤과 낮의 온도차가 심한 지역이나 통풍이 잘되지 않는 곳에서 흔히 발생하며, 농작물뿐만 아니라 관상식물에도 널리 분포하며 항상 발생하기 때문에, 매년 흰가루병에 의한 식물 생장 및 수량 감소는 다른 어떤 병보다도 심하여 농사에 막대한 피해를 일으키는 병원균이다. 이러한 흰가루병원균은 시설재배지에 항시 존재, 발생하기 때문에 화학적인 방법으로는 근절하기가 어려운 병해이다.These pathogens overwinter in the remnants of diseased plants in the form of asymptomatic or mycelium, becoming the primary source of infection, and in plant cultivation, conidia spores become airborne and continue to occur. It often occurs in areas with high temperature difference between night and day or in poorly ventilated areas, and it is widely distributed not only in crops but also in ornamental plants and always occurs. Therefore, the decrease in plant growth and yield caused by powdery mildew every year is worse than any other diseases. It is a pathogen that causes enormous damage to. These powdery pathogens are a disease that is difficult to eradicate by chemical methods because they always exist and occur in facility cultivation.
흰가루병을 방제하기 위해 이미다졸(imidazole), 트리아질(triazole) 등의 스테롤 생합성저해제와 벤즈이미다졸(benzimidazole)계의 살균제, 스트로비루린(strobilurin)계의 화합물 등이 함유된 살균제를 사용되고 있다. 그러나 이러한 화학 살균제를 계속 사용함으로 약제저항성 균들이 발생하게 되었고, 스테롤 생합성저해제와 벤즈이미다졸계의 살균제는 이미 흰가루병에 대해 방제효과가 저해되었거나 이 약제에 저항성을 지닌 흰가루병이 발생했다는 것이 보고된 바 있다.In order to control powdery mildew, sterol biosynthesis inhibitors such as imidazole and triazole, benzimidazole-based fungicides, and strobilurin-based compounds are used. However, the use of these chemical fungicides caused drug-resistant bacteria, and sterol biosynthesis inhibitors and benzimidazole-based fungicides have already been reported to inhibit the control of powdery mildew or to develop powdery mildew resistant to the drug. have.
식물 흰가루병에 대한 방제를 목적으로 새로운 농약 개발이 많이 진행되고 있으나, 농약성분의 85 ~ 90% 가 대기, 토양, 수계에 산포되어 지속적으로 환경을 파괴시키고, 병해충에 내성 및 저항성이 커지며, 인체에 해를 끼치는 문제가 있다.Many new pesticides are being developed for the purpose of controlling plant powdery mildew.However, 85 to 90% of pesticides are scattered in the air, soil, and water, which continuously destroy the environment, and become more resistant and resistant to pests. There is a problem of harm.
이에, 기존살균제에 대한 저항성 발생으로 흰가루병을 방제하는데 많은 어려움을 겪게 되었고, 화학농약의 지속적인 사용 및 남용으로 인해 인간에 대한 직접적인 독성 및 독성의 잔류 등의 문제 발생으로 천연추출물을 이용한 새로운 방제 물질의 개발이 요구되는 실정이다.As a result, resistance to existing fungicides caused a lot of difficulties in controlling powdery mildew, and due to the continuous use and abuse of chemical pesticides, there was a problem such as direct toxicity and residual toxic effects on humans. Development is required.
이러한 배경하에, 본 발명자들은 흰가루병을 유발하는 병원균에 대해 높은 항균활성을 가지는 천연물질을 발굴하기 위해 예의 노력한 결과, 담배 추출물의 흰가루병 방제 효과를 확인하여 본 발명을 완성하였다.Under these backgrounds, the present inventors have made diligent efforts to discover natural substances having high antibacterial activity against pathogens causing powdery mildew, and thus completed the present invention by confirming the powdery mildew control effect of tobacco extract.
본 발명의 목적은, 담배 추출물을 유효성분으로 포함하는 식물 흰가루병 방제용 조성물, 이의 제조방법 및 식물 흰가루병 방제제를 제공하는 것이다.It is an object of the present invention to provide a composition for plant powdery mildew control comprising a tobacco extract as an active ingredient, a method for preparing the same, and a plant powdery mildew control agent.
그러나, 본 발명이 해결하고자 하는 과제는 이상에서 언급한 과제로 제한되지 않으며, 언급되지 않은 또 다른 과제들은 아래의 기재로부터 해당 기술분야의 통상의 지식을 가진 자에게 명확하게 이해될 수 있을 것이다.However, the problem to be solved by the present invention is not limited to the above-mentioned problem, another task that is not mentioned will be clearly understood by those skilled in the art from the following description.
본 발명의 일 실시예에 따르면, 담배 추출물을 유효성분으로 포함하는, 식물 흰가루병 방제용 조성물이 제공된다.According to one embodiment of the invention, there is provided a composition for controlling plant powdery mildew, comprising a tobacco extract as an active ingredient.
일 측에 따르면, 상기 식물 흰가루병 유발균은 포도스파이라 푸스카(Podosphaera fusca)일 수 있다.According to one side, the plant powdery mildew causing bacteria may be Podosphaera fusca (Podosphaera fusca).
일 측에 따르면, 상기 담배 추출물은, 우방초 또는 우설초 담배로부터 추출된 것일 수 있다.According to one side, the tobacco extract, may be one extracted from woobangcho or tongue tongue tobacco.
일 측에 따르면, 상기 담배 추출물은, 담배의 잎, 꽃, 뿌리 또는 줄기로부터 부터 추출된 것일 수 있다.According to one side, the tobacco extract may be extracted from the leaves, flowers, roots or stems of tobacco.
일 측에 따르면, 상기 담배 추출물은, 아세톤, 주정, 탄소수 1~4의 저급 알코올, 에틸 아세테이트 및 1,3-부틸렌글리콜 중에서 선택되는 하나 이상을 추출 용매로 하여 수득한 것일 수 있다.According to one side, the tobacco extract may be obtained by using at least one selected from acetone, alcohol, lower alcohol having 1 to 4 carbon atoms, ethyl acetate and 1,3-butylene glycol as the extraction solvent.
일 측에 따르면, 상기 담배 추출물은, 우설초의 꽃 또는 뿌리의 70% 아세톤 추출물일 수 있다.According to one side, the tobacco extract may be 70% acetone extract of the flower or root of tongue tongue.
본 발명의 다른 일 실시예에 따르면, 본 발명의 일 실시예에 따른 조성물을 포함하는, 식물 흰가루병 방제제가 제공된다.According to another embodiment of the present invention, there is provided a plant powdery mildew control agent comprising a composition according to an embodiment of the present invention.
일 측에 따르면, 상기 방제제는 계면 활성제, 점착제, 증량제 및 영양제로부터 선택된 하나 이상을 포함할 수 있다.According to one side, the control agent may include one or more selected from surfactants, pressure-sensitive adhesives, extenders and nutrients.
일 측에 따르면, 상기 방제제는 입제, 액제, 수화제, 과립 또는 캡슐제 형태인, 식물 흰가루병 방제제.According to one side, the control agent is in the form of granules, liquids, hydrates, granules or capsules, plant powdery mildew control.
본 발명의 또 다른 일 실시예에 따르면, 우설초 또는 우방초 담배를 준비하는 단계; 상기 담배를 추출 용매에 침지하여 추출물을 제조하는 단계; 및 상기 추출물을 여과하여 농축하는 단계; 를 포함하는, 식물 흰가루병 방제용 조성물의 제조방법이 제공된다.According to another embodiment of the present invention, preparing a tongue tongue or woobangcho tobacco; Preparing an extract by dipping the tobacco in an extraction solvent; And concentrating the extract by filtration. Provided is a method for producing a composition for controlling plant powdery mildew.
일 측에 따르면, 상기 여과 및 농축 단계 이후에, 계면 활성제, 점착제, 증량제 또는 영양제를 첨가하는 단계;를 더 포함할 수 있다.According to one side, after the filtration and concentration step, adding a surfactant, pressure-sensitive adhesive, extender or nutrients; may further include.
일 측에 따르면, 상기 추출 용매는, 아세톤, 주정, 탄소수 1~4의 저급 알코올, 에틸 아세테이트 및 1,3-부틸렌글리콜 중에서 선택되는 하나 이상일 수 있다.According to one side, the extraction solvent may be at least one selected from acetone, alcohol, lower alcohol having 1 to 4 carbon atoms, ethyl acetate and 1,3-butylene glycol.
일 측에 따르면, 상기 식물 흰가루병은 포도스파이라 푸스카(Podosphaera fusca)에 의해 유발되는 것일 수 있다.According to one side, the plant powdery mildew may be caused by Podosphaera fusca (Podosphaera fusca).
일 측에 따르면, 상기 추출물은, 담배의 잎, 꽃, 뿌리 또는 줄기로부터 부터 추출된 것일 수 있다.According to one side, the extract may be extracted from the leaves, flowers, roots or stems of tobacco.
본 발명의 식물 흰가루병 방제용 조성물은 포도스파이라 푸스카(Podosphaera fusca)에 우수한 항균 활성을 가지며 환경오염 및 인체 독성이 없어 환경 친화적으로 식물 흰가루병을 방제할 수 있다.The composition for controlling plant powdery mildew of the present invention has excellent antibacterial activity against Podosphaera fusca, and does not have environmental pollution and human toxicity, and thus can control plant powdery mildew.
본 발명의 효과는 상기한 효과로 한정되는 것은 아니며, 본 발명의 상세한 설명 또는 청구범위에 기재된 발명의 구성으로부터 추론 가능한 모든 효과를 포함하는 것으로 이해되어야 한다.It is to be understood that the effects of the present invention are not limited to the above effects, and include all effects deduced from the configuration of the invention described in the detailed description or claims of the present invention.
도 1은 우설초 추출물의 부위별 흰가루병 방제 효과를 나타내는 사진이다.
도 2는 종류 및 부위별 담배 추출물의 메론 흰가루병 방제 효과를 비교하는 사진이다.1 is a photograph showing the powdery mildew control effect of each part of the tongue powder extract.
Figure 2 is a photograph comparing the effect of controlling melon powdery powder of tobacco extract by type and site.
이하에서, 첨부된 도면을 참조하여 실시예들을 상세하게 설명한다. 그러나, 실시예들에는 다양한 변경이 가해질 수 있어서 특허출원의 권리 범위가 이러한 실시예들에 의해 제한되거나 한정되는 것은 아니다. 실시예들에 대한 모든 변경, 균등물 내지 대체물이 권리 범위에 포함되는 것으로 이해되어야 한다.Hereinafter, exemplary embodiments will be described in detail with reference to the accompanying drawings. However, various changes may be made to the embodiments so that the scope of the patent application is not limited or limited by these embodiments. It is to be understood that all changes, equivalents, and substitutes for the embodiments are included in the scope of rights.
실시예에서 사용한 용어는 단지 설명을 목적으로 사용된 것으로, 한정하려는 의도로 해석되어서는 안된다. 단수의 표현은 문맥상 명백하게 다르게 뜻하지 않는 한, 복수의 표현을 포함한다. 본 명세서에서, "포함하다" 또는 "가지다" 등의 용어는 명세서 상에 기재된 특징, 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것이 존재함을 지정하려는 것이지, 하나 또는 그 이상의 다른 특징들이나 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것들의 존재 또는 부가 가능성을 미리 배제하지 않는 것으로 이해되어야 한다.The terminology used herein is for the purpose of description and should not be construed as limiting. Singular expressions include plural expressions unless the context clearly indicates otherwise. In this specification, terms such as "comprise" or "have" are intended to indicate that there is a feature, number, step, action, component, part, or combination thereof described on the specification, and one or more other features. It is to be understood that the present invention does not exclude the possibility of the presence or the addition of numbers, steps, operations, components, components, or a combination thereof.
다르게 정의되지 않는 한, 기술적이거나 과학적인 용어를 포함해서 여기서 사용되는 모든 용어들은 실시예가 속하는 기술 분야에서 통상의 지식을 가진 자에 의해 일반적으로 이해되는 것과 동일한 의미를 가지고 있다. 일반적으로 사용되는 사전에 정의되어 있는 것과 같은 용어들은 관련 기술의 문맥 상 가지는 의미와 일치하는 의미를 가지는 것으로 해석되어야 하며, 본 출원에서 명백하게 정의하지 않는 한, 이상적이거나 과도하게 형식적인 의미로 해석되지 않는다.Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art. Terms such as those defined in the commonly used dictionaries should be construed as having meanings consistent with the meanings in the context of the related art and shall not be construed in ideal or excessively formal meanings unless expressly defined in this application. Do not.
또한, 첨부 도면을 참조하여 설명함에 있어, 도면 부호에 관계없이 동일한 구성 요소는 동일한 참조부호를 부여하고 이에 대한 중복되는 설명은 생략하기로 한다. 실시예를 설명함에 있어서 관련된 공지 기술에 대한 구체적인 설명이 실시예의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우 그 상세한 설명을 생략한다.In addition, in the description with reference to the accompanying drawings, the same components regardless of reference numerals will be given the same reference numerals and duplicate description thereof will be omitted. In the following description of the embodiment, when it is determined that the detailed description of the related known technology may unnecessarily obscure the gist of the embodiment, the detailed description thereof will be omitted.
본 명세서에서 사용되는 용어, “흰가루병”은 곰팡이 균사류가 엉켜 식물체가 회백색을 띠게 되는 병을 의미하는 것으로, 병에 걸린 부위는 흉한 모양으로 뒤틀리면서 잎이나 줄기를 시들게 하고 열매의 질이 떨어지게 된다.As used herein, the term “powdery mildew” refers to a disease in which the fungus mycelium is entangled and the plant becomes grayish white. The diseased area is warped in an unsightly shape, withering leaves or stems, and deteriorating the quality of the fruit. .
본 발명의 일 실시예에 따르면, 담배 추출물을 유효성분으로 포함하는, 식물 흰가루병 방제용 조성물이 제공된다.According to one embodiment of the invention, there is provided a composition for controlling plant powdery mildew, comprising a tobacco extract as an active ingredient.
일 측에 따르면, 상기 식물 흰가루병 유발균은 포도스파이라 푸스카(Podosphaera fusca)일 수 있다. 포도스파이라 푸스카는 식물 병원체를 감염시키는 곰팡이균으로, 포도스파이라 푸스카에 의한 병해로는 오이 흰가루병, 호박 흰가루병, 메론 흰가루병, 수박 흰가루병, 참외 흰가루병, 박 흰가루병, 상추 흰가루병 등이 있다.According to one side, the plant powdery mildew causing bacteria may be Podosphaera fusca (Podosphaera fusca). Grape spira pusca is a fungus that infects plant pathogens. Diseases caused by grape spira pusca include cucumber powdery mildew, pumpkin powdery mildew, melon powdery mildew, watermelon powdery mildew, melon powdery mildew, gourd powdery mildew and lettuce powdery mildew.
본 발명의 조성물에 포함되는 담배 추출물은, 우방초 또는 우설초 담배로부터 추출된 것일 수 있으며, 담배의 잎, 꽃, 뿌리 또는 줄기로부터 부터 추출된 것일 수 있다. 우방초, 우설초는 담배(Nicotiana tabacum)의 재래종에 속하는 담배를 나타낸다.Tobacco extract included in the composition of the present invention, may be extracted from woobangcho or tongue tongue tobacco, may be extracted from tobacco leaves, flowers, roots or stems. Woobangcho and tongue tongue represent tobacco belonging to the conventional species of tobacco (Nicotiana tabacum).
일 측에 따르면, 상기 담배 추출물은, 아세톤, 주정, 탄소수 1~4의 저급 알코올, 에틸 아세테이트 및 1,3-부틸렌글리콜 중에서 선택되는 하나 이상을 추출 용매로 하여 수득한 것일 수 있다.According to one side, the tobacco extract may be obtained by using at least one selected from acetone, alcohol, lower alcohol having 1 to 4 carbon atoms, ethyl acetate and 1,3-butylene glycol as the extraction solvent.
일 측에 따르면, 상기 담배 추출물은, 우설초의 꽃 또는 뿌리의 70% 아세톤 추출물일 수 있다. According to one side, the tobacco extract may be 70% acetone extract of the flower or root of tongue tongue.
본 발명의 다른 일 실시예에 따르면, 본 발명의 일 실시예에 따른 조성물을 포함하는, 식물 흰가루병 방제제가 제공된다.According to another embodiment of the present invention, there is provided a plant powdery mildew control agent comprising a composition according to an embodiment of the present invention.
본 발명의 방제제는 계면 활성제, 점착제, 증량제 및 영양제로부터 선택된 하나 이상을 포함할 수 있다. 본 발명의 방제제는 본 발명의 조성물에, 계면 활성제, 점착제, 증량제 및 영양제로부터 선택된 하나 이상을 더 포함할 수 있다. 계면활성제로는 폴리카복실레이트, 소듐 리그노설포네이트, 칼슘 리그노설포네이트, 소듐 다이알킬 설포석시네이트, 소듐 알킬 아릴 설포네이트, 폴리옥시에틸렌 알킬 페닐 에테르, 소듐 트리폴리포스페이트, 폴리옥시에틸렌 알킬 아릴 포스포릭 에스테르, 폴리옥시에틸렌 알킬 아릴 에테르, 폴리옥시에틸렌 알킬 아릴 폴리머, 폴리옥시알킬온 알킬 페닐 에테르, 폴리옥시에틸렌 노닐 페닐 에테르, 소듐 설포네이트 나프탈렌 포름알데히드, 트리톤 100 및 트윈 80으로부터 선택되는 하나 이상을 사용할 수 있다. 점착제로는 전분, 하이드(hide) 아교, 카제인, 덱스트린, 식물검, 동물 단백질, 식물 단백질, 셀락, 천연 고무, 나트륨 실리케이트, 알키드, 아크릴 에스테르, 폴리아미드, 폴리스티렌, 합성 고무, 폴리비닐알코올, 우레아 수지, 페놀 수지, 불포화 폴리에스테르 수지, 에폭시 수지, 멜라민 수지, 푸란 수지 및 알키드 수지로부터 선택되는 하나 이상을 사용할 수 있다. 증량제 및 영양제로는 소듐알기네이트, 전분, 대두박, 밀기울, 입상섬유질, 유안, 규조토, 제올라이트, 벤토나이트, 탈크, 카올린, 파이로필라이트, 화이트카본, 당류, 콩가루, 쌀, 밀, 황토 및 덱스트린으로부터 선택되는 하나 이상을 사용할 수 있다. 이외에도, 방제제의 제형, 이용 목적에 따라 부동액, 분산제, 증점제, 붕해제 등을 더 포함할 수 있다.The control agents of the present invention may include one or more selected from surfactants, tackifiers, extenders and nutrients. The control agent of the present invention may further comprise at least one selected from surfactants, pressure-sensitive adhesives, extenders and nutrients in the composition of the present invention. Surfactants include polycarboxylate, sodium lignosulfonate, calcium lignosulfonate, sodium dialkyl sulfosuccinate, sodium alkyl aryl sulfonate, polyoxyethylene alkyl phenyl ether, sodium tripolyphosphate, polyoxyethylene alkyl aryl At least one selected from phosphoric esters, polyoxyethylene alkyl aryl ethers, polyoxyethylene alkyl aryl polymers, polyoxyalkylone alkyl phenyl ethers, polyoxyethylene nonyl phenyl ethers, sodium sulfonate naphthalene formaldehyde, Triton 100 and Tween 80 Can be used. Adhesives include starch, hide glue, casein, dextrin, plant gum, animal protein, plant protein, shellac, natural rubber, sodium silicate, alkyd, acrylic ester, polyamide, polystyrene, synthetic rubber, polyvinyl alcohol, urea One or more selected from resins, phenolic resins, unsaturated polyester resins, epoxy resins, melamine resins, furan resins, and alkyd resins can be used. Extenders and nutrients include sodium alginate, starch, soybean meal, bran, granular fiber, yuan, diatomaceous earth, zeolite, bentonite, talc, kaolin, pyrophyllite, white carbon, sugars, soy flour, rice, wheat, ocher and dextrin One or more selected may be used. In addition, it may further include an antifreeze, a dispersant, a thickener, a disintegrant, etc. according to the formulation of the control agent, the purpose of use.
본 발명의 방제제는 입제, 액제, 수화제, 과립 또는 캡슐제 형태로 제조될 수 있으나, 이에 한정되는 것은 아니다. 액제와 수화제는 필요에 따라 1/1000로 희석하여 사용할 수 있다.The control agent of the present invention may be prepared in the form of granules, liquids, hydrates, granules or capsules, but is not limited thereto. Liquids and hydrating agents can be diluted to 1/1000 as needed.
본 발명의 또 다른 일 실시예에 따르면, 우설초 또는 우방초 담배를 준비하는 단계; 상기 담배를 추출 용매에 침지하여 추출물을 제조하는 단계; 및 상기 추출물을 여과하여 농축하는 단계; 를 포함하는, 식물 흰가루병 방제용 조성물의 제조방법이 제공된다.According to another embodiment of the present invention, preparing a tongue tongue or woobangcho tobacco; Preparing an extract by dipping the tobacco in an extraction solvent; And concentrating the extract by filtration. Provided is a method for producing a composition for controlling plant powdery mildew.
일 측에 따르면, 상기 여과 및 농축 단계 이후에, 계면 활성제, 점착제, 증량제 또는 영양제를 첨가하는 단계;를 더 포함할 수 있다.According to one side, after the filtration and concentration step, adding a surfactant, pressure-sensitive adhesive, extender or nutrients; may further include.
이하, 실시예를 통하여 본 발명을 보다 상세히 설명하기로 한다. 하기 실시예는 본 발명을 예시하기 위한 목적으로 기술된 것으로서, 본 발명의 범위가 이에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to Examples. The following examples are described for the purpose of illustrating the present invention, but the scope of the present invention is not limited thereto.
실시예Example 1: 담배 추출물의 제조 1: Preparation of Tobacco Extract
우방초와 우설초를 준비하여 꽃, 잎, 줄기 및 뿌리 부위로 나누어 잘게 절단한다. 절단된 꽃, 잎, 줄기 및 뿌리 100g을 1L 70% 아세톤 용액에 침지시켜 상온에서 7일 동안 추출하고, 이를 여과한 후 농축한 다음 50% MeOH에 용해시킨 최종 추출물을 제조하였다. Prepare woobangcho and tongue tongue and divide into flowers, leaves, stems and roots and cut them finely. 100 g of cut flowers, leaves, stems, and roots were immersed in a 1L 70% acetone solution, extracted for 7 days at room temperature, filtered, concentrated, and dissolved in 50% MeOH to prepare a final extract.
실시예Example 2: 담배 추출물의 흰가루병 실내 방제 효과 2: Effect of Tobacco Extracts on Indoor Powder Control
실시예 1에서 제조된 우설초의 꽃, 잎, 줄기 및 뿌리 추출물을 물(DW)에 희석하여 5mg/ml의 농도로 준비한다. 시설 재배지에서 발생한 메론 흰가루병 이병 잎을 원형 디스크 (Φ 28mm) 형태로 절단하여 표면에 담배 추출물을 분무한 후, 페트리디쉬의 살균수에 적신 여과지 위에 올려 놓고 26℃로 고정된 배양기(incubator)에서 5일 동안 배양하였다. 배양 후 이병 정도를 육안으로 비교 측정한 결과, 추출물과 동일한 양의 물을 분무한 무처리군과 비교하여 담배 추출물을 분무한 경우 발병 면적이 감소하는 것을 확인할 수 있었다. 도 1에서 나타난 바와 같이, 우설초의 꽃, 잎 추출물의 효과가 가장 우수한 것을 확인할 수 있었다.Flower, leaf, stem and root extracts of tongue tongue prepared in Example 1 are diluted in water (DW) to prepare a concentration of 5mg / ml. Cut melon powdery mildew leaves from plant cultivation into circular disk (Φ 28mm), spray tobacco extract on the surface, place on filter paper soaked in petri dish sterilized water, and incubate fixed at 26 ℃. Incubated for days. As a result of visually measuring the degree of disease after incubation, it was confirmed that the onset area was reduced when the tobacco extract was sprayed compared to the untreated group sprayed with the same amount of water as the extract. As shown in Figure 1, it was confirmed that the effect of the flower, leaf extract of tongue tongue.
실시예Example 3: 담배 추출물의 메론 흰가루병 방제 효과 3: Effects of Tobacco Extracts on Melon Powder Disease
실시예 1에서 제조된 담배 추출물을 5mg/ml 농도로 희석하여 시설 재배지에서 재배된 메론 흰가루병 발병 초기인 11엽 ~ 20엽에 1주일 간격으로 2회 살포하였다. 발병도 조사는 농촌진흥청 연구조사분석 기준에 준하여 담배 추출물 살포 1주일 후 10개 엽에 대해 발병 면적률 (이병율)이 1~20%인 경우는 1, 21~50%는 2, 51~70%는 3, 71~90%는 4, 90% 이상인 경우는 5로 표시하고, 하기 식 1을 이용하여 발병도를 산출하였다.Tobacco extract prepared in Example 1 was diluted to a concentration of 5mg / ml and was sprayed twice a week to 11 ~ 20 leaves, which is the beginning of the melon powdery mildew disease cultivated in the facility plantation. The incidence survey was based on the Rural Research Administration's analysis and analysis criteria, and 1, 21 ~ 50%, 2, 51 ~ 70% for 10 leaves after 1 week of tobacco extract application. Is 3, 71 to 90% is 4, when the 90% or more represented by 5, the incidence was calculated using the following formula (1).
[식 1][Equation 1]
담배 추출물의 흰가루병 방제가는 무처리군의 발병도 대비 감소 정도를 하기 식 2를 이용하여 산출하였다.Powder powder control value of the tobacco extract was calculated using the following equation 2 to the degree of reduction compared to the incidence of the untreated group.
[식 2][Equation 2]
시설 재배지에서의 흰가루병 방제 효과를 확인하기 위해 포장시험을 실시한 결과, 도 2에 나타난 바와 같이 부위별 추출물 모두 흰가루병 방제 효과를 나타내는 것으로 확인 되었다. 또한, 농촌진흥청 연구조사분석 기준에 준하여 발명도 및 방제가를 분석한 결과, 하기 표 1에 나타난 바와 같이 실시예 1에서 제조된 모든 담배 추출물은 45% 이상의 흰가루병 방제 효과를 나타내었으며, 특히 우설초의 꽃, 잎 추출물은 70% 이상의 우수한 흰가루병 방제 효과를 나타내었다.As a result of the field test to confirm the effect of powdery mildew control at the plantation site, as shown in Figure 2, it was confirmed that the extracts of each part exhibits powdery mildew control effect. In addition, as a result of analyzing the invention and control value according to RDA research and analysis criteria, as shown in Table 1, all tobacco extracts produced in Example 1 showed more than 45% powdery mildew control effect, in particular, The flower and leaf extracts showed excellent powdery mildew control effects of more than 70%.
위와 같은 결과는 우방초, 우설초 담배 추출물이 흰가루병 방제용 조성물로 우수한 효과를 나타낸 수 있음을 시사한다.The above results suggest that tobacco extracts of woobangcho and tongue tongue can exhibit excellent effects as a composition for powdery mildew control.
이상과 같이 실시예들이 비록 한정된 도면에 의해 설명되었으나, 해당 기술분야에서 통상의 지식을 가진 자라면 상기를 기초로 다양한 기술적 수정 및 변형을 적용할 수 있다. 예를 들어, 설명된 기술들이 설명된 방법과 다른 순서로 수행되거나, 및/또는 설명된 시스템, 구조, 장치, 회로 등의 구성요소들이 설명된 방법과 다른 형태로 결합 또는 조합되거나, 다른 구성요소 또는 균등물에 의하여 대치되거나 치환되더라도 적절한 결과가 달성될 수 있다.Although the embodiments have been described with reference to the accompanying drawings, those skilled in the art may apply various technical modifications and variations based on the above. For example, the described techniques may be performed in a different order than the described method, and / or components of the described systems, structures, devices, circuits, etc. may be combined or combined in a different form than the described method, or other components Or even if replaced or substituted by equivalents, an appropriate result can be achieved.
그러므로, 다른 구현들, 다른 실시예들 및 특허청구범위와 균등한 것들도 후술하는 청구범위의 범위에 속한다.Therefore, other implementations, other embodiments, and equivalents to the claims are within the scope of the following claims.
Claims (14)
Tobacco extract containing as an active ingredient, plant powdery mildew control composition.
상기 식물 흰가루병 유발균은 포도스파이라 푸스카(Podosphaera fusca)인, 식물 흰가루병 방제용 조성물.
The method of claim 1,
The plant powdery mildew-inducing bacterium is grape spira (Podosphaera fusca), the composition for controlling plant powdery mildew.
상기 담배 추출물은, 우방초 또는 우설초 담배로부터 추출된 것인, 식물 흰가루병 방제용 조성물.
The method of claim 1,
The tobacco extract is extracted from woobangcho or tongue tongue tobacco, plant powdery mildew control composition.
상기 담배 추출물은, 담배의 잎, 꽃, 뿌리 또는 줄기로부터 추출된 것인, 식물 흰가루병 방제용 조성물.
The method of claim 3,
The tobacco extract is extracted from the leaves, flowers, roots or stems of tobacco, plant powdery mildew control composition.
상기 담배 추출물은, 아세톤, 주정, 탄소수 1~4의 저급 알코올, 에틸 아세테이트 및 1,3-부틸렌글리콜 중에서 선택되는 하나 이상을 추출 용매로 하여 수득한 것인, 식물 흰가루병 방제용 조성물.
The method of claim 3,
The tobacco extract is obtained by using at least one selected from acetone, alcohol, lower alcohol having 1 to 4 carbon atoms, ethyl acetate, and 1,3-butylene glycol as an extraction solvent, plant powder control composition.
상기 담배 추출물은, 우설초의 꽃 또는 뿌리의 70% 아세톤 추출물인, 식물 흰가루병 방제용 조성물.
The method of claim 4, wherein
The tobacco extract is 70% acetone extract of the flower or root of tongue tongue, plant powder control composition.
Plant powdery mildew control agent containing the composition of any one of Claims 1-6.
상기 방제제는 계면 활성제, 점착제, 증량제 및 영양제로부터 선택된 하나 이상을 포함하는, 식물 흰가루병 방제제.
The method of claim 7, wherein
The control agent comprises at least one selected from surfactants, pressure-sensitive adhesives, extenders and nutrients, plant powdery mildew control agent.
상기 방제제는 입제, 액제, 수화제, 과립 또는 캡슐제 형태인, 식물 흰가루병 방제제.
The method of claim 7, wherein
The control agent is in the form of granules, liquids, hydrates, granules or capsules, plant powdery mildew control.
상기 담배를 추출 용매에 침지하여 추출물을 제조하는 단계; 및
상기 추출물을 여과하여 농축하는 단계; 를 포함하는, 식물 흰가루병 방제용 조성물의 제조방법.
Preparing tongue tongue or cowweed tobacco;
Preparing an extract by dipping the tobacco in an extraction solvent; And
Filtering and extracting the extract; A method of producing a composition for controlling plant powdery mildew, comprising.
상기 여과 및 농축 단계 이후에,
계면 활성제, 점착제, 증량제 또는 영양제를 첨가하는 단계;를 더 포함하는, 식물 흰가루병 방제용 조성물의 제조방법.
The method of claim 10,
After the filtration and concentration steps,
Adding a surfactant, pressure-sensitive adhesive, extender or nutrients; further comprising, manufacturing method for controlling plant powdery disease.
상기 추출 용매는, 아세톤, 주정, 탄소수 1~4의 저급 알코올, 에틸 아세테이트 및 1,3-부틸렌글리콜 중에서 선택되는 하나 이상인, 식물 흰가루병 방제용 조성물의 제조방법.
The method of claim 10,
The extraction solvent is at least one selected from acetone, spirits, lower alcohols having 1 to 4 carbon atoms, ethyl acetate and 1,3-butylene glycol, the method for producing a composition for controlling plant powdery mildew.
상기 식물 흰가루병은, 포도스파이라 푸스카(Podosphaera fusca)에 의해 유발되는 것인, 식물 흰가루병 방제용 조성물의 제조방법.
The method according to any one of claims 10 to 12,
The plant powdery mildew, which is caused by Podosphaera fusca (Podosphaera fusca), a method for producing a composition for controlling plant powdery mildew.
상기 추출물은, 담배의 잎, 꽃, 뿌리 또는 줄기로부터 추출된 것인, 식물 흰가루병 방제용 조성물의 제조방법.
The method according to any one of claims 10 to 12,
The extract is a method for producing a composition for controlling plant powdery mildew, which is extracted from tobacco leaves, flowers, roots or stems.
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| CN1268298A (en) * | 2000-04-21 | 2000-10-04 | 侯永林 | Green composite pesticide |
| JP2005289846A (en) * | 2004-03-31 | 2005-10-20 | Hirokazu Shimoji | Insecticide |
| KR20090045960A (en) * | 2007-10-30 | 2009-05-11 | 전라남도 | Botanical pesticide from plant extract with sustainable emulsifying agent to control of aphids |
| KR20160058293A (en) * | 2014-11-14 | 2016-05-25 | 대한민국(농촌진흥청장) | Microbial agent possessing antifungal activity against ginseng root rot pathogens containing soil actinomycete strain |
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| CN1268298A (en) * | 2000-04-21 | 2000-10-04 | 侯永林 | Green composite pesticide |
| JP2005289846A (en) * | 2004-03-31 | 2005-10-20 | Hirokazu Shimoji | Insecticide |
| KR20090045960A (en) * | 2007-10-30 | 2009-05-11 | 전라남도 | Botanical pesticide from plant extract with sustainable emulsifying agent to control of aphids |
| KR20160058293A (en) * | 2014-11-14 | 2016-05-25 | 대한민국(농촌진흥청장) | Microbial agent possessing antifungal activity against ginseng root rot pathogens containing soil actinomycete strain |
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