KR20190086421A - Companion animal nutrient containing collagen extract and method for producing the same - Google Patents
Companion animal nutrient containing collagen extract and method for producing the same Download PDFInfo
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Abstract
Description
본 발명은 콜라겐추출물을 포함한 반려동물 영양제 및 그 제조방법에 관한 것으로 닯발 및 명태로부터 추출한 콜라겐추출물을 이용하여 반려동물의 영양제로 제조하거나 반려동물 영양제사료로 제공하기 위한 것이다.The present invention relates to a companion animal nutrient including a collagen extract and a method for producing the same. The present invention provides a nutritional supplement for a companion animal or a companion animal nutrient feed using a collagen extract extracted from a mulberry and a pollack.
최근 국내외 반려동물 사육은 소득 수준이 높아지고, 노령화 및 1인 가구 등 핵가족화되면서 급격하게 증가하고 있는 추에에 있다. 미국의 경우 2015년 개 또는 고양이를 키우는 가구가 53.5%, 개와 고양이를 함께 키우는 가구는 12.7%이고, 기타 Pets을 키우는 가구가 10.5%로 전체 가구의 76.7%가 반려동물을 키우고 있는 것으로 나타나고 있다.In recent years, domestic and overseas companion animal breeding has been growing rapidly as income level is rising, aging and becoming a nuclear family such as single person household. In the United States, 53.5% of dogs and cats are raised by dogs and cats, and 12.7% of dogs and cats are raised by dogs and cats in 2015.
우리나라의 경우에도 노인가구 및 1인가구가 증가하면서 반려동물 사육가구는 크게 증가하고 있다. 2015년 우리나라의 전체 가구중 21.8%가 반려동물을 키우기 있는 것으로 조사되고 있다.In Korea, the number of households raised by companion animals is also increasing, as the number of elderly households and households increases. In 2015, 21.8% of all households in Korea are surveyed to raise companion animals.
그러나 반려동물 가구의 급격한 증가에도 우리나라 반려동물의 영양제는 크게 산업화되지 못하고 있어 외국의 영양제 제품에 밀리고 있으며, 그나마 국내 반려동물의 사료 시장에서도 소비자들로부터 외면 받고 외국 사료제품이 크게 잠식하고 있는 실정이다. 반려동물의 사료 수출입 현황을 보면 2015년 47,863톤이 수입된 반면, 수출은 6,488톤 정도로 수입 비중이 높아 국내 반려동물 사료시장의 약 58%가 수입품에 의존하고 있는 실정이다.However, even with the rapid increase of companion households, the nutrients of domestic companion animals are not highly industrialized, and they are being pushed out by foreign nutritional products. In the feed market of domestic companion animals, . In terms of imports and exports of companion animals, 47,863 tons were imported in 2015, while exports accounted for 6,488 tons of imported goods, which means that about 58% of the domestic animal feed market depends on imports.
또한 반려동물 산업에서 반려동물 영양제를 포함한 사료시장의 규모가 2012년 약 33%인 2,970억원이고, 반려동물 의료시장이 31%인 2,790억원, 반려동물 용품시장이 20%인 1,800억원 정도로 조사되었으며, 그동안 급격한 반려동물 사육 가구수의 증가로 사료시장의 규모는 크게 증가한 것으로 추정된다.In addition, in the companion animal industry, the size of the feed market including companion animal nutrients is estimated to be 337%, 297 billion won in 2012, 279 billion won in the companion animal care market and 20 billion won in the companion animal products market, It is estimated that the size of the feed market has increased significantly due to the rapid increase in the number of companion animals raised.
현재 반려동물을 키우는 가구의 경우 반려동물에 대한 이해부족으로 반려동물에게 적합한 사료를 제공하지 못하고, 사람이 먹는 음식물 등을 먹여서 많은 반려동물들이 여러가지 질환이 있는 경우가 많이 있다.In the case of households currently raising a companion animal, the companion animal can not provide adequate food for the companion animal due to lack of understanding about the companion animal. In many cases, many companion animals have various diseases because they feed the food to be eaten.
반려동물이 선호하는 제품은 비만, 피부질환, 면역력 부족 등 실내 생활 위주의 반려동물에게 자주 발생하는 질환 예방 및 치료 효과가 있는 제품이나 노령 애경에게 자주 발생하는 관절, 치주질환 예방 및 지료효과가 있는 제품이 선호되고 있으나 국내에는 이러한 제품을 찾아보기 힘들고, 외국 제품의 경우 고가이기 때문에 일반적으로 반려동물에게 제공하는 것이 쉽지 않은 실정이다.The products preferred by the companion animal are those products that have the effect of prevention and treatment of diseases frequently occurring in companion animals such as obesity, skin diseases, immunity deficiency, etc., Although products are preferred, it is difficult to find such products in Korea, and foreign products are expensive, so it is generally not easy to provide them to companion animals.
본원발명은 상기와 같은 문제점을 해결하기 위하여 고급의 영양제 성분인 콜라겐추출물을 반려동믈에게 제공하기 위한 것이다. 또한 닭발 및 명태에서 유래된 콜라겐을 저분자 펩타이드로 분해된 콜라겐 추출물을 영양제로 제공하여 비만 및 피부질환, 면역력 부족 등의 질환을 예방하고, 관절 및 치주질환을 예방하기 위한 것이다.In order to solve the above-mentioned problems, the present invention provides a collagen extract, which is a high-quality nutritional ingredient, to a companion dog. The present invention also provides a collagen extract obtained by degrading collagen derived from chicken hair and pollack as a low molecular peptide as a nutrient to prevent diseases such as obesity, skin diseases and immunity, and to prevent joints and periodontal diseases.
상기와 같은 콜라겐 추출물을 맥강, 대두분말 또는 옥수수분말을 포함하는 탄수화물 성분에 포함시켜 콜라겐 추출물이 함유된 영양제 사료를 반려동물에게 제공하기 위한 것이다.The collagen extract is contained in a carbohydrate component including a manganese, soybean powder or corn powder to provide a companion animal with a nutritional feed containing collagen extract.
본 발명은 상기와 같은 목적을 달성하기 위하여 닭발과 명태로부터 콜라겐을 발효공법으로 추출하고 상기 추출된 저분자 콜라겐을 반려동물의 영양제로 제공하고, 또한 영양제 사료에 포함시켜 개, 고양이 및 관상용 어류 등 반려동물에게 먹이 형태로 제공한다. In order to achieve the above-mentioned object, the present invention provides a method of extracting collagen from chicken broth and pollack by a fermentation method, providing the extracted low molecular collagen as a nutrient for companion animals, Provide animals with food.
본원발명에서 사용되는 콜라겐은 닭발과 명태의 혼합물에서 유래된 것이다. 닭발은 닭을 도축하고 나오는 부산물로서 닭발에는 피부, 혈관, 뼈, 치아, 근육 등 인체를 구성하는 모든 결합조직의 단백질 주원료인 콜라겐이 많이 함유되어 있다. The collagen used in the present invention is derived from a mixture of chicken leg and pollack. Chicken feet are a by-product of slaughtering chickens. Chicken feet contain a lot of collagen, which is the main ingredient of proteins in all connective tissues that make up the human body such as skin, blood vessels, bones, teeth, and muscles.
참고로 보통 사람 체중의 16%정도가 단백질로 구성되어 있으며, 이중 약 3분의 1이 콜라겐으로 이루어져 있다. 콜라겐은 형태적으로 가늘고 긴 막대 모양을 하고 있으며 우리 몸을 형태를 지탱하는 역할을 한다. 콜라겐은 연골세포, 근섬유세포, 골세포, 피부섬유아세포 등에서 만들어진다. 피부에서 콜라겐의 구성량은 약 70%에 이르며, 진피의 경우 90%이상을 차지한다. 관절을 건강하게 하고, 피부를 탄력 있게 유지시켜주는 기능을 한다.For reference, about 16% of normal human body weight is made up of protein, and about one third of it is made up of collagen. Collagen has a long, thin, long rod shape and serves to support the shape of our body. Collagen is made from cartilage cells, muscle fiber cells, bone cells, and skin fibroblasts. The amount of collagen in the skin is about 70%, and in the case of the dermis it accounts for more than 90%. It keeps the joints healthy and keeps the skin elastic.
동물의 피부를 열수로 끓여 얻어진 유도단백질이 젤라틴이다. 젤라틴은 엄밀한 의미에서 콜라겐이라고 부를 수 없다. 콜라겐을 구성하는 아미노산으로는 프롤린, 옥시프롤린, 글리신, 글루탐산 등이 있다. 골질 또한 골 조직의 세포간질을 형성하는 물질로서 콜라겐의 일종이다. 콜라겐은 20대부터 체내에서 생성이 저하되어 40대에는 절반 이하로 떨어진다. 돼지껍질이나 족발, 도가니탕 등에 콜라겐이 들어있고, 특히 닭발에 많이 함유되어 있다.Gelatin is an inductive protein obtained by boiling an animal's skin with hot water. Gelatin can not be called collagen in a strict sense. Amino acids constituting the collagen include proline, oxyproline, glycine, glutamic acid, and the like. Bone is also a type of collagen that forms the interstitium of bone tissue. Collagen is produced in the body from the 20s and declines to less than half in the 40s. Collagen is contained in pig skin, paws, and potato, and is especially contained in chicken hair.
상기 콜라겐 추출물은 반려동물의 영양제사료로 제공할 수 있는데 영양제사료는 맥강, 대두분말 또는 옥수수분말에 콜라겐 추출물을 혼합 건조하여 제조한다. 상기 반려동물 영양제사료는 맥강, 대두분말 또는 옥수수분말 중 하나 또는 2이상의 혼합물 100중량부에 콜라겐추출물 1~35중량부를 포함하는 것이 바람직하며, 상기 혼합물에 비타민 C, 글루코사민, MSM(법제유황), 칼슘, 발효효소 또는/및 사과 추출물을 더 포함할 수 있으며, 이 경우 상기 맥강, 대두분말 또는 옥수수분말 중 하나 또는 2이상의 혼합물 100중량부에 비타민 C 0.01~10중량부, MSM 0.1~2중량부, 글루코사민 0.5~5중량부, 칼슘 1~3중량부, 발효효소 0.5~10중량부, 덱스트린 0.5~5중량부, 유산균 0.1~3중량부, 효모균 0.1~3중량부, 고초균 0.1~3중량부, 영지버섯균사체 1~5중량부, 상황버섯균사체 1~5중량부, 사과추출물 0.5~5중량부, 홍삼분말 0.5~3중량부 또는 유산균 0.1~1중량부로 구성되는 성분 하나 또는 2 이상의 성분을 혼합하여 반려동물 영양제로 제조한다.The collagen extract may be provided as a nutritional feed for companion animals. The nutritional feed is prepared by mixing and drying a collagen extract in a manganese, soybean powder or corn powder. Preferably, the feed of the companion animal nutrient includes 1 to 35 parts by weight of a collagen extract in 100 parts by weight of one or more of a mixture of one or more of manganese, soybean powder or corn powder, and vitamin C, glucosamine, MSM (legal sulfur) Wherein 0.01 to 10 parts by weight of vitamin C and 0.1 to 2 parts by weight of MSM are added to 100 parts by weight of one or more of the above-mentioned manganese, soybean powder or corn powder, 0.5 to 5 parts by weight of glucosamine, 1 to 3 parts by weight of calcium, 0.5 to 10 parts by weight of fermentation enzyme, 0.5 to 5 parts by weight of dextrin, 0.1 to 3 parts by weight of lactic acid bacteria, 0.1 to 3 parts by weight of yeast, 0.1 to 3 parts by weight of Bacillus subtilis 1 to 5 parts by weight of a mushroom mycelia, 1 to 5 parts by weight of mycelia of mushroom, 0.5 to 5 parts by weight of an apple extract, 0.5 to 3 parts by weight of red ginseng powder or 0.1 to 1 part by weight of a lactic acid bacterium. Mixed and prepared as a companion animal nutrient.
본원발명은 실내 생활 위주의 개, 고양이 등 반려동물에게 자주 발생하는 비만, 피부질환, 면역력부족 등에 탁월한 효과가 있으며, 또한 반려견의 탈모도 예방할 수 있는 효과가 있다.The present invention has an excellent effect on obesity, skin diseases, immunity and lack of immunity, which frequently occur in companion animals such as dogs and cats, which are mainly for indoor living, and also can prevent hair loss of dogs.
특히 반려동물의 손상된 피부 즉, 상처가 난 경우 또는 질병 등으로 트러블이 생기 피부의 회복에 탁월한 효과를 제공한다.In particular, it provides an excellent effect in the recovery of damaged skin of companion animals, that is, in the case of a wound or a disease caused by trouble.
또한 음식에 민감한 일부 반려동물에게 제공되어 곡물 또는 가금류 고기의 사료에 대한 과민방응을 예방할 수 있는 효과가 있다.It is also provided to some companion animals that are sensitive to food, which can prevent irritation of the feed of grain or poultry meat.
또한 노령 반려동물에게 자주 발생하는 관절질환 및 치주질환을 예방하거나 치료하는 효과를 제공한다.It also provides the effect of preventing or treating joint disease and periodontal disease, which frequently occur in aged companion animals.
이하 실시예를 통해 본 발명을 보다 상세하게 설명한다. 다만 이러한 실시예에 의해 본 발명이 한정되는 것은 아니며, 실시예에 인용되는 수치는 본 발명의 수치범위에서 선택하여 본 발명의 이해를 돕기 위한 하나의 실시예에 불과한 것이다.Hereinafter, the present invention will be described in more detail with reference to examples. However, the present invention is not limited to these embodiments, and numerical values cited in the embodiments are merely one example for facilitating understanding of the present invention by selecting within the numerical range of the present invention.
<닭발명태 혼합물을 이용한 콜라겐추출물의 제조>≪ Preparation of Collagen Extract Using Chicken Feet < RTI ID = 0.0 >
닭발 100g에는 170∼180Kcal 정도의 열량이 있고, 영양성분으로는 탄수화물 11%, 단백질 21%, 지방 67%가 함유되어 있다. 비타민 B1, B2, B6, C, E 등이 함유되어 있고, 기타 미량원소로는 나이아신 1.20mg, 나트륨 30.00mg, 아연 0.1mg, 인 21.0mg, 지질 0.20mg, 철분 0.5mg, 칼륨 65.0mg, 칼슘 2.0mg, 콜레스테롤 5.40mg, 회분 0.20mg 등이 함유되어 있다.100g chicken breast 100g calories about 170 ~ 180Kcal of nutrients, carbohydrates 11%, 21% protein, 67% fat is contained. Vitamin B1, B2, B6, C, and E, and other trace elements include niacin 1.20 mg, sodium 30.00 mg, zinc 0.1 mg, phosphorus 21.0 mg, lipid 0.20 mg, iron 0.5 mg, potassium 65.0 mg, calcium 2.0mg, 5.40mg of cholesterol, 0.20mg of ash and the like.
명태에는 단백질, 칼슘, 인, 철분, 비타민 A 및 나이아신 등의 영양성분이 풍부하게 함유되어 있고, 특히 명태살에 칼슘 성분이 풍부하게 들어 있다. Pollack is rich in protein, calcium, phosphorus, iron, vitamin A, and niacin. Especially, it contains abundant calcium in pollock.
본원발명의 닭발과 명태를 이용한 콜라겐 추출물은 닭발과 명태에서 추출한다. 먼저, 도축하고 남은 닭발을 세척한 후 발톱과 비늘 등의 이물질을 제거한다. The collagen extracts of the invention of the present invention are extracted from chicken legs and pollocks. First, slaughter and clean the remaining chicken feet, and then remove foreign materials such as claws and scales.
물로 세척한 명태는 1~3Cm의 길이로 절단한다. 본 발명에서 명태는 생태를 건조시킨 것으로 황태, 건태, 북어, 노가리 및 코다리 등을 총칭하는 의미로 사용하는 것으로 한다.The polished rice washed with water is cut to a length of 1 to 3 cm. In the present invention, pollack is dried in ecology and generally used in the meaning of "hwangtae", "kuntae", "korea", "nogari", and "kodari".
상기 세척하고 이물질을 제거한 닭발과 명태를 물과 함께 중탕솥에 넣고 100∼130℃, 1.2~2.0 기압의 압력하에서 20~40분 동안 1차 가열하며, 바람직하게는 110℃, 1.5기압에서 30분 동안 가열하는 것이 가장 바람직하다The chicken feet and the pollack which have been cleaned and the foreign matter are removed are placed in a hot water pot together with water and then heated at a temperature of 100 to 130 ° C under a pressure of 1.2 to 2.0 atm for 20 to 40 minutes and preferably at 110 ° C and 1.5 atm for 30 minutes Lt; RTI ID = 0.0 >
상기 1차 가열한 닭발과 명태의 혼합물에 1~3배의 물을 더 넣고 온도 70~100℃의 온도에서 20~80시간 동안 2차 가열하여 끓이면 발효효과를 얻을 수 있다. 상기와 같이 닭발 및 명태를 1차 및 2차 가열하여 콜라겐추출물을 제조한다.The fermentation effect can be obtained by adding 1 to 3 times more water to the mixture of the primary heated chicken leg and the pollack and boiling the mixture at a temperature of 70 to 100 ° C for 20 to 80 hours. As described above, chicken broth and pollack are first and secondly heated to prepare a collagen extract.
상기와 같이 제조된 닭발 및 명태를 이용한 콜라겐추출물은 저분자 펩타이드의 척도가 되는 하이드록시플로린(Hydroxyproline)의 함량이 기존의 콜라겐 제품보다 10~50% 이상 많이 함유되어 있어 반려동물의 영양제 또는 영양제사료로 사용하는 경우 흡수가 용이한 장점이 있다.Since the content of hydroxyproline, which is a measure of low molecular peptide, is more than 10 ~ 50% higher than that of the existing collagen products, the collagen extract using the chicken broth and pollack prepared as described above can be used as a nutrient or nutrient feed It has an advantage of easy absorption when used.
<콜라겐추출물을 이용한 반려동물 영양제의 제조>≪ Preparation of companion animal nutrient using collagen extract >
상기 닭발로부터 추출한 콜라겐추출물 또는, 닭발과 명태를 혼합하여 추출한 콜라겐추출물은 반려동물 영양제로 제조할 수 있으며, 반려동물 영양제로 제조하는 경우 다양한 영양소과 혼합하여 제조된다. The collagen extract extracted from the chicken leg or the collagen extract obtained by mixing the chicken leg and the pollack can be prepared as a companion animal nutrient, and when it is prepared as a companion animal nutrient, it is mixed with various nutrients.
또한 상기 콜라겐추출물에 아래의 영양소를 혼합하여 반려동물 영양제를 제조하는 경우 콜라겐추출물은 부형제 100중량부에 5~50중량부의 비율로 혼합하여 제조하며, 상기 부형제로는 화곡류, 서류, 두류 등의 곡물, 또는 육류 및 생선 등이 사용된다. 상기 반려동물 영양제에 혼합되어 사용될 수 있는 영양소에는 비타민 C, 글루코사민, MSM, 칼슘, 발효효소, 덱스트린, 유산균, 효모균, 고초균, 영지버섯균사체, 상황버섯균사체, 사과추출물 및 홍삼분말을 하나 또는 2 이상의 성분을 혼합하여 사용한다.When the collagen extract is mixed with the following nutrients to produce a companion animal nutrient, the collagen extract is prepared by mixing 5 to 50 parts by weight of the collagen extract in 100 parts by weight of the excipient. The excipient may be at least one selected from the group consisting of cereals, Cereals, or meat and fish. The nutrients that can be mixed with the companion animal nutrients include one or more of vitamin C, glucosamine, MSM, calcium, fermentation enzyme, dextrin, lactic acid bacteria, yeast, Bacillus subtilis, mushroom mycelium, mushroom mycelium, apple extract, The ingredients are mixed and used.
이경우 비타민 C 0.01~10중량부, MSM 0.1~2중량부, 글루코사민 0.5~5중량부, 칼슘 1~3중량부, 발효효소 0.5~10중량부, 덱스트린 0.5~5중량부, 유산균 0.1~3중량부, 효모균 0.1~3중량부, 고초균 0.1~3중량부, 영지버섯균사체 1~5중량부, 상황버섯균사체 1~5중량부, 사과추출물 0.5~5중량부, 홍삼분말 0.5~3중량부 또는 유산균 0.1~1중량부로 구성되는 성분 하나 또는 2 이상의 성분을 상기 콜라겐추출물 100중량부에 혼합하여 반려동물 영양제로 제조한다.In this case, 0.01 to 10 parts by weight of vitamin C, 0.1 to 2 parts by weight of MSM, 0.5 to 5 parts by weight of glucosamine, 1 to 3 parts by weight of calcium, 0.5 to 10 parts by weight of fermentation enzyme, 0.5 to 5 parts by weight of dextrin, 0.1 to 3 parts by weight of yeast, 0.1 to 3 parts by weight of Bacillus subtilis, 1 to 5 parts by weight of mycelia of gingko mushrooms, 1 to 5 parts by weight of mycelia of mushroom, 0.5 to 5 parts by weight of apple extract, 0.5 to 3 parts by weight of red ginseng powder, 0.1 to 1 part by weight of a lactic acid bacterium is mixed with 100 parts by weight of the collagen extract to prepare a companion animal nutrient.
상기 콜라겐추출물에 효모균, 유산균, 고초균 및 발효효소를 상기 비율로 혼합하여 제조된 반려동물 영양제를 반려동물에게 급이하면 락토바실러스 등 반려동물의 장내 유익균 활동을 도와 반려동물의 장을 쾌적하게 하고, 유해물질요소와 크레아티닌의 생성을 줄이고 체외로 배출시켜 반려동물의 장을 건강하게 해준다. When the companion animal nutrient prepared by mixing yeast, lactic acid bacteria, Bacillus subtilis, and fermentation enzyme in the above ratio into the above collagen extract is fed to the companion animal, the companion animal's activity of the companion animal such as Lactobacillus is promoted, Reduces the production of harmful substances and creatinine, and excretes them out of the body, thereby improving the health of companion animals.
상기 영지버섯균사체 및 상황버섯균사체는 베타글루칸의 함량이 높아 항염 및 항암효과와 면역력을 증진시켜 주며, 또한 반려동물 체내 신진대사를 활발하게 하여 내장 기능 향상 및 개선에 뛰어난 효과가 있다. 상기 합량비율은 다른 성분의 조화을 위해 최적의 비율로 혼합한 프리미엄 반려동물 영양제로 손색이 없다.The mushroom mycelia and the mushroom mycelium have a high beta-glucan content, thereby enhancing the anti-inflammatory and anti-cancer effects and immunity. Also, the mushroom mycelium and the mushroom mycelium have an excellent effect on enhancing and improving the visceral function by activating metabolism in the companion animal. The combination ratio is a premium companion animal nutrient mixed at an optimum ratio for the harmony of the other ingredients.
또한 홍삼분말을 상기 비율로 콜라겐추출액과 혼합하여 영양제로 제조하는 경우 콜레스테롤 생합성 촉진, 호르몬분비촉진, 항암작용 및 면역력 증진 등의 효과가 있으며, 반려동물의 체질을 건강하게 유지시켜줄 수 있도록 상기 비율로 혼합하여 준다.In addition, when red ginseng powder is mixed with the collagen extract at the above ratio, it is effective as a nutritional supplement to promote cholesterol biosynthesis, promote hormone secretion, anticancer activity and immunity. In order to keep the composition of the companion animal healthy, Mix.
상기 콜라겐추출물을 이용한 반려동물 영양제는 실내 생활이 많은 반려동물에게 자주발생하는 비만, 피부질환 및 면역력 부족 등에 탁월한 효과가 있으며, 노령 애견에게 자주 발생하는 관절질환이나 치주질환의 예방 및 치료에 효과가 있다.The collagen extract of the present invention has excellent effects on obesity, skin diseases, immunity and lack of immunity, which are often found in companion animals living in a living room. It is effective for prevention and treatment of joint diseases and periodontal diseases have.
상기 콜라겐추출물 영양제의 아미노산 함량은 다음과 같이 분석되었다.The amino acid content of the collagen extract nutrient was analyzed as follows.
[시료 및 표준물질 제조방법] [Method of preparing samples and reference materials]
검액 조제방법 Preparing the sample solution
액상시료를 동결건조 및 분말화하여 검액으로 사용함(O-1) Liquid sample is lyophilized and powdered and used as sample solution (O-1)
6N HCl을 준비한 후 동결건조 된 시료 100mg/mL로 녹여줌 Prepare 6N HCl and dissolve with 100 mg / mL of lyophilized sample
Teflon tape으로 잘 감싼 후 dry oven을 이용하여 110℃에서 24시간 동안 반응시켜줌 Wrapped with Teflon tape and reacted at 110 ° C for 24 hours using a dry oven
24시간 경과 후 질소가스를 공급하여 flushing후 3차 증류수로 혼합하여 필터링 후 HPLC 분석함 After 24 hours, nitrogen gas was supplied and flushing was performed, followed by mixing with tertiary distilled water, followed by filtering and HPLC analysis
표준물질 제조방법 Standard material manufacturing method
실험에 사용한 지표 성분인 아미노산은 Pickering사에서 구매하여 사용함 The amino acid used as an indicator in the experiment was purchased from Pickering.
표준원액을 Sodium diluent(Na220)로 1/2, 1/4배의 농도로 희석하여 검량선을 작성 Dilute standard stock solution to 1/2 and 1/4 concentration with sodium diluent (Na220) to prepare calibration curve
*1) n.d: not detected(불검출)* 1) n.d: not detected (not detected)
<콜라겐추출물을 이용한 반려동물 영양제사료의 제조>≪ Preparation of feed animal feeds using collagen extract >
상기 닭발로부터 추출한 콜라겐추출물 또는 닭발과 명태를 혼합하여 추출한 콜라겐추출물을 이용하여 반려동물 영양제사료를 제조할 수 있으며, 반려동물 영양제사료는 탄수화물의 공급원으로 옥수수나 대두, 보리, 밀 등의 곡물을 사용하며, 단백질공급원으로는 닭, 오리 등의 가금류 고기가 사용된다.The collagen extract extracted from the chicken legs or the collagen extract extracted from the mixture of chicken legs and pollack can be used to produce feeds for companion animal nutrients. The feed of the companion animal nutrients can be used as a source of carbohydrates such as corn, soybean, barley and wheat And chicken, duck, and other poultry meat are used as protein sources.
본 발명에서는 상기 콜라겐추출물을 사용하여 상기 탄수화물 공급원 및 단백질 공급원과 혼합하여 반려동물 영양제사료를 제조한다.In the present invention, the collagen extract is mixed with the carbohydrate source and the protein source to prepare a feed animal feed.
탄수화물 공급원에 콜라겐추출물을 혼합하여 반려동물 영양제사료를 제조하는 경우 맥강, 옥수수분말 또는 대두분말 등 곡물분말을 하나 또는 2 이상을 혼합한 혼합물 100중량부에 상기 콜라겐추출물 5~30중량부를 혼합하여 과립 또는 환의 형태로 반려동물 영양제사료를 제조하며, 상기 반려동물의 영양제사료의 수분함량은 10~15% 정도로 하는 것이 반려동물에게 급이하였을 때 잘 먹으면서 보관에도 용이하다. 상기 곡물분말은 90~130℃의 온도에서 10~30분 동안 볶은 다음 과립 또는 분말로 제조하여 사용하는 것이 바람직하다. When preparing a feed animal feed mixture by mixing collagen extract with a carbohydrate source, 5 to 30 parts by weight of the collagen extract is mixed with 100 parts by weight of a mixture of one or more cereal powders such as cornstarch, corn flour or soybean flour, Or a loin, and the moisture content of the nutrient feed of the companion animal is set to about 10 to 15%, which is easy to store while being eaten well when the animal is fed. The grain powder is preferably roasted at a temperature of 90 to 130 ° C for 10 to 30 minutes and then used as granules or powder.
상기 반려동물 영양제사료에 사용되는 콜라겐추출물을 제조할 때 닭발과 함께 명태를 사용함으로써 명태의 생선맛을 가미하여 반려동물들이 더 선호하는 영양제사료가 된다.When preparing the collagen extracts used for the feed of the companion animal nutrients, using the pollocks together with the chicken roots, the companion animals are more preferred nutritional feeds by adding the fish flavor of the pollacks.
탄수화물 공급원과 단백질공급원을 혼합하여 반려동물 영양제사료를 제조하는 경우 맥강, 옥수수분말 또는 대두분말 등 곡물분말 중 하나 또는 2 이상을 혼합한 혼합물 100중량부에 콜라겐추출물 3~20중량부와 닭 또는 오리 등의 가금류 고기 1~5중량부를 사용하여 반려동물 영양제사료를 제조하며, 상기 반려동물의 영양제사료의 수분 함량은 8~13 중량% 정도 함유하는 것이 반려동물이 먹기 좋으며, 영양제사료의 보관도 바람직하다.When 100 parts by weight of a mixture of one or more of cereal powder such as manganese, corn powder or soybean powder is mixed with 3 to 20 parts by weight of a collagen extract and 100 parts by weight of chicken or duck And 1 to 5 parts by weight of poultry meat are used to prepare a companion animal nutrient feed. The moisture content of the nutrient feed of the companion animal is about 8 to 13% by weight, which is good for companion animals to eat, Do.
상기 탄수화물공급원을 이용한 반려동물 영양제사료와 탄수화물공급원 및 단백질공급원을 이용한 반려동물 영양제사료에 홍삼분말을 더 포함하여 프리미엄급 반려동물 영양제사료를 제조할 수 있으며, 이경우 홍삼분말은 1~3중량부를 더 혼합하여 제조한다. The feed of the carbohydrate may be further supplemented with the red ginseng powder in the feed of the companion animal nutrients using the feed of the companion animal nutrients, the source of the carbohydrates and the source of the protein. In this case, 1 to 3 parts by weight of the red ginseng powder .
[실시예 1] [Example 1]
닭발로부터 추출한 콜라겐추출물을 이용한 반려동물 영양제는 다음과 같이 제조한다.Companion animal nutrients using collagen extracts extracted from chicken wings are prepared as follows.
먼저 세척하고 이물질을 제거한 닭발 1Kg을 물 2Kg과 함께 중탕솥에 넣고 110℃, 1.5 기압의 압력하에서 30분 동안 1차 가열한 다음, 상기 1차 가열한 닭발에 1차 가열한 닭발의 2배의 물을 더 넣고 90℃의 온도에서 30시간 동안 2차 가열하여 끓인다. 상기와 같이 닭발을 1차 및 2차 가열하여 콜라겐추출물을 제조한다.First, 1 kg of chicken legs which had been washed and debris was put in a hot water pot together with 2 kg of water and heated for 30 minutes at 110 ° C. under a pressure of 1.5 atm. Then, Water is further added and the mixture is boiled by secondary heating at a temperature of 90 ° C for 30 hours. The chicken hair is first and secondly heated as described above to prepare a collagen extract.
상기 콜라겐추출물를 부형제에 혼합한 후 건조하여 과립형태의 반려동물 영양제를 제조한다.The collagen extract is mixed with an excipient and then dried to prepare a granular animal feed nutrient.
[실시예 2] [Example 2]
닭발과 명태를 혼합하여 추출한 콜라겐추출물을 이용한 반려동물 영양제는 다음과 같이 제조한다.The collagen extract obtained by mixing chicken broth with pollack is prepared as follows.
먼저 세척하고 이물질을 제거한 닭발 1Kg과 물로 세척한 명태 500g을 물 3Kg과 함께 중탕솥에 넣고 110℃, 1.5 기압의 압력하에서 30분 동안 1차 가열한 다음, 상기 1차 가열한 닭발명태 혼합물에 상기 혼합물의 2배의 물을 더 넣고 90℃의 온도에서 30시간 동안 2차 가열하여 끓인다. 상기와 같이 닭발 및 명태를 1차 및 2차 가열하여 콜라겐추출물을 제조한다.First, 1 kg of chicken legs washed with water and 500 g of polish roasted with water were added to a hot water pot with 3 kg of water and then heated at 110 ° C. under a pressure of 1.5 atm for 30 minutes. Then, the above- Add 2 times more water of the mixture and boil for 30 hours at 90 ℃ for 2 hours. As described above, chicken broth and pollack are first and secondly heated to prepare a collagen extract.
상기 콜라겐추출물를 부형제에 혼합한 후 건조하여 과립형태의 반려동물 영양제를 제조한다.The collagen extract is mixed with an excipient and then dried to prepare a granular animal feed nutrient.
[실시예 3] [Example 3]
홍삼분말을 더 포함하는 반려동물 영양제를 제조하는 경우, 실시예 2의 콜라겐추출물 100중량부와 홍삼분말 0.5~3중량부를 부형제에 혼합한 후 건조하여 과립형태의 프리미엄급 반려동물 영양제를 제조한다.100 parts by weight of the collagen extract of Example 2 and 0.5 to 3 parts by weight of red ginseng powder are mixed with excipients and dried to prepare a granular form of premium companion animal nutrient.
[실시예 4][Example 4]
탄수화물 공급원에 상기 실시예 2의 콜라겐추출물을 혼합하여 반려동물 영양제사료를 제조하는 경우 대두분말 100중량부에 콜라겐추출물 15중량부를 혼합하여 과립상태의 반려동물 영양사료를 제조한다. 상기 대두분말은 100℃의 온도에서 20분 동안 볶은 다음 분말로 제조하여 사용하는 것이 바람직하다. When a feed of carbohydrate is mixed with the collagen extract of Example 2, 15 parts by weight of a collagen extract is mixed with 100 parts by weight of soybean powder to prepare a granular feed animal feed. The soybean powder is preferably roasted at a temperature of 100 ° C for 20 minutes and then used as a powder.
[실시예 5][Example 5]
탄수화물 공급원으로 대두와 단백질공급원으로 닭고기를 사용하고, 실시예 2의 콜라겐추출물을 혼합하여 반려동물 영양제사료를 제조하는 경우 대두분말 100중량부에 콜라겐추출물 15중량부, 닭고기 2중량부를 혼합하여 과립상태의 반려동물 영양사료를 제조한다.When chicken meat is used as a source of carbohydrate and chicken meat is used as a source of protein and collagen extract of Example 2 is mixed to prepare a companion animal nutrient feed, 15 parts by weight of collagen extract and 2 parts by weight of chicken are mixed with 100 parts by weight of soybean powder, Of animal feed.
[반려동물 피부질환 효능 조사][Study on the efficacy of companion animal skin disease]
상기 실시예들의 반려동물 영양제와 영양제사료를 1 ~2년생 포메라니안(Pomeranian)에 1일 3회, 1회 150g의 영양제 및 영양제사료를 보통의 사료와 함께 4주 동안 급이한 경우와 상기 영양제 및 영양제사료를 급이하지 않은 대조구를 비교하여 피부상태를 조사하였다. 상기 실험에서 각 군에는 포메라니안 5마리를 1군으로 하여 실험하였으며, 트러블이 발생된 포메라니안의 피부상태를 5단계(5점 척도법)로 조사한 결과 다음과 같은 결과를 얻었다.When the feed of the companion animal nutrients and the nutrient feed of the above examples is fed to the Pomeranian for 1 to 2 years, three times a day, once for 150 g of the nutritional and nutritional feeds for four weeks with the ordinary feed, The skin condition was examined by comparing the control without feeding the nutrient feed. In the above experiment, five groups of pomeranian were used as the first group, and the skin condition of the pomeranian occurred in five steps (5 point scaling method). The results were as follows.
상기 5점 척도법은 1 : 심한트러블, 2: 트러블, 3 : 보통, 4 : 양호, 5 : 극히 양호로 구분한다.The 5-point scale method is divided into 1: severe trouble, 2: trouble, 3: normal, 4: good, and 5: extremely good.
상기 실시예의 표에서 보는 바와 같이 본원발명의 영양제 또는 영양제사료를 급이한 반려동물의 피부질환 및 피부트러블 완화 효과가 크게 양호한 것으로 나타났다. As shown in the table of the above example, the skin diseases and skin troubles of the companion animals fed with the nutritional or nutritional feed of the present invention were greatly improved.
Claims (4)
상기 반려동물 영양제의 제조방법은 부형제 100중량부와 콜라겐추출물 5~50중량부가 혼합된 혼합물에 비타민 C, 글루코사민, MSM, 칼슘, 발효효소, 덱스트린, 유산균, 효모균, 고초균, 영지버섯균사체, 상황버섯균사체, 사과추출물 및 홍삼분말을 하나 또는 2 이상의 성분을 혼합하여 제조하되,
상기 부형제는 화곡류, 서류, 두류로 구성되는 곡물을 사용하고,
상기 콜라겐추출물은 닭발과 명태를 물과 함께 중탕솥에 넣고 100~130℃, 1.2~2.0 기압의 압력하에서 20~40분 동안 1차 가열한 다음, 상기 1차 가열한 닭발과 명태의 혼합물에 1~3배의 물을 더 넣고 온도 70~100℃에서 20~80시간 동안 2차 가열하여 제조한 콜라겐추출물을 사용하여 제조하는 것을 특징으로 하는 반려동물 영양제의 제조방법
A method for producing collagen-containing companion animal nutrient,
The method for preparing the companion animal nutrient according to the present invention is characterized in that 100 parts by weight of an excipient and 5 to 50 parts by weight of a collagen extract are mixed with a mixture of vitamin C, glucosamine, MSM, calcium, fermentation enzyme, dextrin, lactic acid bacteria, yeast, Mycelium, apple extract, and red ginseng powder by mixing one or two or more ingredients,
The excipient may be selected from the group consisting of grain, paper and grain,
The collagen extracts were prepared by first heating the chicken legs and pollacks together with water in a hot water pot and heating them at a temperature of 100 to 130 ° C under a pressure of 1.2 to 2.0 atm for 20 to 40 minutes and adding 1 To 3 times of water and further heating the mixture at a temperature of 70 to 100 DEG C for 20 to 80 hours to prepare a collagen extract
상기 반려동물 영양제의 제조방법은 상기 부형제 100중량부와 콜라겐추출물 5~50중량부가 혼합된 혼합물에 비타민 C 0.01~10중량부, MSM 0.1~2중량부, 글루코사민 0.5~5중량부, 칼슘 1~3중량부, 발효효소 0.5~10중량부, 덱스트린 0.5~5중량부, 유산균 0.1~3중량부, 효모균 0.1~3중량부, 고초균 0.1~3중량부, 영지버섯균사체 1~5중량부, 상황버섯균사체 1~5중량부, 사과추출물 0.5~5중량부, 홍삼분말 0.5~3중량부 또는 유산균 0.1~1중량부로 구성되는 성분 하나 또는 2 이상의 성분을 혼합하여 제조하는 것을 특징으로 하는 반려동물 영양제의 제조방법
The method according to claim 1,
The method for preparing a companion animal nutrient according to claim 1, wherein 0.01 to 10 parts by weight of vitamin C, 0.1 to 2 parts by weight of MSM, 0.5 to 5 parts by weight of glucosamine, 1 to 5 parts by weight of calcium, 3 to 5 parts by weight of lactic acid bacteria, 0.1 to 3 parts by weight of lactic acid bacteria, 0.1 to 3 parts by weight of yeast bacteria, 0.1 to 3 parts by weight of Bacillus subtilis, 1 to 5 parts by weight of mycelia of gingko mushrooms, 1 to 5 parts by weight of mushroom mycelia, 0.5 to 5 parts by weight of apple extract, 0.5 to 3 parts by weight of red ginseng powder or 0.1 to 1 part by weight of lactic acid bacterium. ≪ / RTI &
상기 반려동물 영양제사료의 제조방법은 맥강, 대두분말 또는 옥수수분말 중 하나 또는 2 이상을 혼합한 혼합물 100중량부에 콜라겐추출물 3~20중량부, 닭 또는 오리를 포함하는 가금류 고기 1~5중량부, 홍삼분말 1~3중량부를 혼합하여 제조하되,
상기 맥강, 대두분말 또는 옥수수분말 중 하나 또는 2 이상을 혼합한 혼합물은 90~130℃의 온도에서 10~30분 동안 볶은 다음 과립 또는 분말로 제조하여 사용하고,
상기 콜라겐추출물은 닭발과 명태를 물과 함께 중탕솥에 넣고 100~130℃에서 20~40분 동안 1차 가열한 다음, 상기 1차 가열한 닭발과 명태의 혼합물에 1~3배의 물을 더 넣고 온도 70~100℃에서 20~80시간 동안 2차 가열하여 제조한 콜라겐추출물을 사용하여 제조하는 것을 특징으로 하는 반려동물 영양제사료의 제조방법
A method for preparing a companion animal nutrient feed comprising collagen extract,
The method for preparing the companion animal nutrient feed is characterized by comprising the step of adding 3 to 20 parts by weight of a collagen extract to 1 to 5 parts by weight of poultry meat containing chicken or duck in 100 parts by weight of a mixture of one or more of manganese, , And 1 to 3 parts by weight of red ginseng powder,
The mixture of one or more of the above-mentioned manganese, soybean powder or corn powder is roasted at a temperature of 90 to 130 ° C for 10 to 30 minutes and then used as granules or powder.
The collagen extract was prepared by adding chicken broth and pollack to a hot water pot with water and then heating the mixture at 100 to 130 ° C for 20 to 40 minutes and then adding 1 to 3 times more water to the mixture of the first- And then heating the mixture at a temperature of 70 to 100 ° C for 20 to 80 hours to prepare a collagen extract.
상기 반려동물의 영양제사료의 수분 함량은 8~13 중량%를 함유하도록 제조하는 것을 특징으로 하는 반려동물 영양제사료의 제조방법The method of claim 3,
Characterized in that the moisture content of the nutritive feed of said companion animal is made to contain 8 to 13% by weight
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