KR20190071017A - Manufacturing method of Health Food using honeybee pollen - Google Patents
Manufacturing method of Health Food using honeybee pollen Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/065—Microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Jellies, Jams, And Syrups (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
본 발명은 꿀벌 화분을 이용한 건강식품 제조방법에 관한 것으로, 특히 분말화와 발효로 인해 꿀벌 화분의 영양물질을 효율적으로 이용할 수 있는 꿀벌 화분 발효액을 이용하여 건강식품을 제조함으로써, 꿀벌 화분의 유효성분을 누구나 편리하게 상용할 수 있고, 식품으로 이용할 수 있는 꿀벌 화분을 이용한 건강식품 제조방법에 관한 것이다.The present invention relates to a method of manufacturing a health food using a bee pollen, and more particularly, to a health food by using a bee pollen fermentation broth capable of effectively utilizing nutritive substances of bee pollen due to pulverization and fermentation, The present invention relates to a method for manufacturing a health food using bee pollen which can be conveniently used by anyone and can be used as food.
꽃의 수술에서 나오는 일종의 생식세포인 화분(花粉)은 우수한 항균효과와 면포 감소효과를 인정받아 예로부터 풍기예방, 만성설사 및 종기와 피부 습진의 치료제로 사용되어 왔으며, 현재에도 화장품 원괴나 의료용 치료제, 피부외용제의 용도로 다양하게 사용되고 있다. 또한 화분은 신체 각 부위의 활동을 조절하며 신진대사 작용을 원활하게 하며 식물과 동물의 성장을 촉진하는 물질이 있다. 특히, 환절기 꽃가루 알레르기 예방 효과가 있고 해로운 공해 물질을 청소하는 작용을 한다. 또한 최근에는 단백질을 비롯한 비타민, 미네랄, 지방산, 호르몬, 효소 및 조효소 등이 다양하게 함유되어 있다는 것이 밝혀짐에 따라 식품의 첨가물로서도 많이 이용되고 있는 물질이다.Pollen, a kind of reproductive cells derived from flower surgery, has been used as a treatment for airway prevention, chronic diarrhea, boils and skin eczema since ancient times due to its excellent antimicrobial effect and reducing effect of cottonseed. , And as an external preparation for skin. Pollen also controls the activity of each part of the body, facilitates metabolism, and promotes the growth of plants and animals. In particular, it has the effect of preventing the allergic pollen allergy and it works to clean harmful pollutants. Recently, it has been widely used as food additives because it has been found that various proteins, vitamins, minerals, fatty acids, hormones, enzymes, and coenzymes are contained in various forms.
꿀벌 화분은 화분, 벌의 타액 및 꿀로 이루어진 덩어리 형태로써 수십~수백만 개의 화분 입자들로 구성되어 있다. 꿀벌 화분은 우리 몸에 필수적인 비타민과 미네랄의 훌륭한 공급원이며, 스테미너와 체력을 높여주는 것으로 알려지고 있다. 또한 천연 노화방지제의 주요 공급원이어서 퇴행성 질환 및 기타 질병의 원인이 되는 건강 저해 활동을 억제해주며, 신경장애, 동맥경화증, 정장작용, 간손상 방어, 향균효과에도 효과가 있는 것으로 알려져 있다.Bee pollen is a lump of pollen, bee saliva and honey, consisting of tens or millions of pollen particles. Bee pollen is a great source of essential vitamins and minerals in our bodies and is known to increase stamina and physical fitness. It is also a major source of natural antioxidants and is known to inhibit the health inhibitory activities that cause degenerative diseases and other diseases. It is also known to have effects on neuropathy, arteriosclerosis, rectal action, liver damage defense, and antibacterial effect.
상기와 같은 꿀벌 화분은 크게 외피와 내피의 2층의 껍질에 둘러싸인 구조로 되어 있는데, 외피의 경우 여러 분해 효소나 강한 산에 의하여 파괴되지 않고 유기용매에 대해서도 매우 안정하다는 특징이 있기 때문에 꿀벌 화분을 섭취시 배설물까지 그대로 보존되어 내피 속에 있는 꿀벌 화분의 영양물질을 효율적으로 이용하는데 장애가 되어 왔다. 또한, 꿀벌 화분은 선호도가 떨어지는 풍미를 가지고 있어 섭취를 꺼리는 경우가 많다. 따라서, 꿀벌 화분을 이용한 식품제조 및 가공에 많은 제한점이 있는 실정이다.The honey bee pollen as described above is largely surrounded by the shells of the two layers of the envelope and endothelium. In the case of the envelope, it is not destroyed by various decomposing enzymes or strong acids and is very stable against organic solvents. In the case of ingestion, the excrement has been preserved and it has become an impediment to the effective utilization of nutrients in bee pollen in the endothelium. In addition, honey bee pollen has a low flavor preference, and is often reluctant to consume. Therefore, there are many limitations in food manufacturing and processing using bee pollen.
프로폴리스(propolis)는 꿀벌이 자신의 생존과 번식을 위해 여러 식물에서 뽑아낸 수지와 같은 물질에 자신의 침과 효소 등을 섞어서 만든 물질로, 성분으로는 유기물과 미네랄이 가장 많은데, 미네랄, 비타민, 아미노산, 지방, 유기산, 플라보노이드 등은 세포대사에 중요한 역할을 하며, 테르펜류 등은 항암 작용을 한다. 주요한 효능으로는 항염증, 항산화, 항바이러스, 항알레르기 등이 있으며, 그 외에도 생체면역강화와 세포재생작용을 하며, 강력한 살균력으로 천연 식품보존제로 사용된다. 에탄올로 추출한 프로폴리스의 경우 0.01~0.03%에서 항균 효과를 나타내며, 물로 추출한 프로폴리스는 0.03~1.0%에서 대부분 항균 효과가 있다.Propolis is a substance made by mixing bees with their own needles and enzymes in a resin-like substance extracted from various plants for their survival and reproduction. Organic matter and minerals are the most common constituents, and minerals, vitamins , Amino acids, fats, organic acids, and flavonoids play important roles in cell metabolism, and terpenes and the like have anticancer activities. Its main effects are anti-inflammation, antioxidant, antiviral and antiallergic. In addition, it strengthens the body's immune system and regenerates the cells, and is used as a natural food preservative with strong sterilizing power. The propolis extracted with ethanol has an antibacterial effect at 0.01 ~ 0.03%, and the propolis extracted with water has an antibacterial effect at 0.03 ~ 1.0%.
벌꿀은 고대로부터 자양 강장, 진정, 해독, 피로회복, 위장장애, 기침, 피부 건조, 습진, 변비, 신경쇠약, 숙취, 콜레스테롤, 체중증가, 혈당 감소, 대사 균형 유지 등에 사용되어 왔다. 또한, 미용과 건강에 좋고, 비타민 B군, 특히 B6이 풍부하게 함유되어 있다. 이외에도 꿀은 한약의 쓴맛을 완화시켜 줌으로써 한방에서는 교미제로 널리 사용된다. Honey has been used since ancient times for nourishment, sedation, detoxification, fatigue, gastrointestinal disorders, cough, dry skin, eczema, constipation, nervous breakdown, hangover, cholesterol, weight gain, It is also good for beauty and health, and is rich in vitamin B group, especially B6. In addition, honey is used widely as a mating agent in the oriental medicine by alleviating the bitter taste of herbal medicine.
로얄 제리는 꿀벌 유충의 영양 섭취에 사용되는 꿀벌의 분비물로, 젊은 일벌의 머리 부분에 있는 인두선(咽頭腺)에서 분비되며, 일벌이 될 애벌레를 포함한 집단 내 모든 애벌레에게 먹이로 제공된다. 벌들은 여왕벌이 필요하게 되면, 선택된 한 애벌레에게 생후 첫 4일간 오로지 로얄 제리만, 그것도 대량으로 공급한다. 이렇게 초기에 집중적으로 영양 공급을 하면, 그 애벌레는 알을 낳을 수 있을 만큼 성숙된 난소를 포함해 여왕벌의 형태를 갖추게 된다. "여왕벌이 될 애벌레 및 여왕벌만이 로얄 제리를 먹는다"는 잘못된 상식도 유포되어 있지만, 사실은 모든 애벌레가 로얄 제리를 먹으며 여왕벌을 제외한 성충은 먹지 않는 것이다. 여왕벌은 평생 로얄 제리만 먹고 사는데 일벌보다 몸집이 2배 이상, 수명이 30배 이상이나 된다. 로얄 제리는 단백질, 당질이 주가 되고, 비타민 A, B1, B2 가 풍부하고 수분 62.2%, 단백질 10.4%, 지질 0.7%, 당질 26.1%, 회분 0.6%, 효소, 호르몬, 스테로이드 등으로 구성되어 있다. 로얄 제리를 피부에 도포시 피부 표면의 수분을 적당하게 유지시켜 주고, 피부의 대사를 좋게 해서 탄력 있는 피부로 만들어 주며, 강력한 살균력으로 인한 항염증 작용을 하는 성분이 있어서 피부의 더러움을 깨끗이 없애주고, 피부 트러블을 예방하는 효과가 있다. 또한, 효소작용, 항균 작용, 항종양 작용, 호르몬에 대한 작용, 혈류량 증가하는 효과가 있다. 한 연구에서는 생쥐에 4주간 5 mg, 10 mg을 3일 간격으로 근육 주사하였을 때, 10~20%의 높은 증체량을 나타내었으며, 일반 쥐에 4주간 0.01%씩 첨가하여 급여하였을 때 48%의 높은 증체량을 나타내었다. Royal jelly is a secretion of bees used for nutritional intake of bee larvae, secreted from the pharyngeal gland at the head of a young worker bee, and fed to all larvae in the population, including larvae to be worker bees. When the bees need a queen bee, they only supply a large amount of royal jelly to the selected larvae for the first four days of life. In this early intensive nutrition, the larvae have a form of queen bee, including mature ovaries that can lay eggs. There is a common misconception that "only the caterpillar and queen bee will eat royal jelly," but in fact all larvae eat royal jelly and do not eat adults except queen bee. The queen bee lives only for the life of royal jelly, which is more than twice the size of the worker bees, and the life span is more than 30 times. Royal jelly is mainly composed of proteins and saccharides. It is rich in vitamins A, B1 and B2, and consists of 62.2% moisture, 10.4% protein, 0.7% lipid, 26.1% sugar, 0.6% ash, enzyme, hormone and steroid. Royal Jelly is applied to the skin to maintain proper moisture on the surface of the skin, to improve the metabolism of the skin to make elastic skin, and anti-inflammatory effect due to strong sterilizing power, , It is effective to prevent skin trouble. In addition, there is an effect of increasing enzyme activity, antimicrobial action, antitumor action, action on hormone, and blood flow. In one study, mice were injected intramuscularly at 5 mg and 10 mg for 3 weeks with 4, 5, and 10 mg / kg body weight, respectively, and the body weight gain was 10 ~ 20% Weight gain.
화분을 이용한 건강식품 제조방법과 관련하여 선행기술로는, 대한민국 공개특허공보 제10-2004-0025226호 "화분 발효추출물을 함유하는 식품보존제"(공개일자 : 2004.03.24)가 개시되어 있으나, 본 발명의 꿀벌 화분을 이용한 건강식품 제조방법과는 상이하다.As a prior art related to a method of manufacturing a healthy food using a flowerpot, Korean Patent Publication No. 10-2004-0025226 entitled " Food preservative containing a pollen fermented extract "(published on Mar. 24, 2004) Is different from the method of manufacturing a health food using bee pollen of the present invention.
본 발명의 목적은 영양성분을 용이하게 섭취할 수 있고 풍미가 개선된 꿀벌 화분 발효액을 이용하여 꿀벌 화분의 유효성분을 누구나 편리하게 상용할 수 있는 꿀벌 화분을 이용한 건강식품 제조방법을 제공하는 데 있다.It is an object of the present invention to provide a method of manufacturing a health food using a honey bee pollen which can be conveniently used by anyone using an effective ingredient of a honey bee pollen using a bee pollen fermentation broth which can easily ingest nutritional components and has improved flavor .
상기의 목적을 달성하기 위하여 본 발명의 꿀벌 화분을 이용한 건강식품 제조방법은, 꿀벌 화분을 분쇄하여 분말화하는 꿀벌화분 분말화단계와, 상기 꿀벌화분 분말화단계에서 분말화한 벌꿀 화분 8~12 중량부, 정제수 82~90 중량부, 아스퍼질러스 나이거(Aspergillus niger) 1~3 중량부 및 사카로마이세스 세레비지애(Saccharomyces cerevisiae) 1~3 중량부를 혼합하는 제1혼합단계와, 상기 혼합액을 100~200 rpm, 25~35℃에서 10~14시간 발효시켜 제조된 꿀벌 화분 발효액을 여과하여 준비하는 꿀벌화분 발효액 여과단계와, 프로폴리스 원괴를 추출하고 여과하여 준비하는 프로폴리스 추출단계와, 상기 꿀벌화분 발효액 여과단계에서 여과된 꿀벌 화분 발효액 0.7~1.1 중량부, 상기 프로폴리스 추출단계에서 추출된 프로폴리스 1.6~2.0 중량부, 벌꿀 8~12 중량부, 로얄 제리 0.05~0.15 중량부 및 정제수 85~90 중량부를 넣고 혼합하는 제2혼합단계와, 상기 제2혼합단계에서 생성된 혼합액을 여과, 살균 및 포장하는 단계를 구비한 것을 특징으로 한다.In order to accomplish the above object, the present invention provides a method for manufacturing a health food using a bee pollen, comprising the steps of: pulverizing a honey bee pollen and pulverizing the honey bee pollen; and a honey bee pollinating step 82 to 90 parts by weight of purified water, 1 to 3 parts by weight of Aspergillus niger and 1 to 3 parts by weight of Saccharomyces cerevisiae , A bee pollen fermentation filtration step for preparing a bee pollen fermentation broth prepared by fermenting the mixture solution at 100 to 200 rpm at 25 to 35 ° C for 10 to 14 hours and a step of extracting propolis and filtration to prepare a propolis extract step 0.7 to 1.1 parts by weight of a bee pollen fermentation filtrate filtered in the honeycomb fermentation broth filtration step, 1.6 to 2.0 parts by weight of propolis extracted in the propolis extracting step, 8 to 12 parts by weight of honey, 0.05 to 0.15 parts by weight of royal jelly, And 85 to 90 parts by weight of purified water are mixed and mixed, and a step of filtering, sterilizing and packing the mixed solution produced in the second mixing step.
본 발명의 꿀벌 화분을 이용한 건강식품 제조방법은 분말화와 발효로 인해 꿀벌 화분의 영양물질을 효율적으로 이용할 수 있는 꿀벌 화분 발효액을 이용하여 건강식품을 제조함으로써, 꿀벌 화분의 유효성분을 누구나 편리하게 상용할 수 있는 식품으로 사용할 수 있다.The method of manufacturing a health food using bee pollen according to the present invention is a method for producing a health food by using a honey bee pollen fermentation liquid which can efficiently utilize nutritive substances of bee pollen due to powdering and fermentation, It can be used as a commercially available food.
도 1은 본 발명의 꿀벌 화분을 이용한 건강식품 제조방법의 순서도,
도 2는 발효 미생물에 따른 꿀벌 화분 발효액의 선호도를 도시한 도표,
도 3은 발효 시간에 따른 꿀벌 화분 발효액의 선호도를 도시한 도표,
도 4는 본 발명에 의해 제조된 꿀벌 화분을 이용한 건강식품과 프로폴리스의 항균 효과를 비교한 도표,
도 5는 본 발명에 의해 제조된 꿀벌 화분을 이용한 건강식품과 프로폴리스의 전자공여능을 비교한 도표,
도 6은 본 발명에 의해 제조된 꿀벌 화분을 이용한 건강식품의 복용 전후에 신장, 체중 및 가슴둘레를 측정한 도표,
도 7은 본 발명에 의해 제조된 꿀벌 화분을 이용한 건강식품의 복용 전후에 혈압을 측정한 도표,
도 8은 본 발명에 의해 제조된 꿀벌 화분을 이용한 건강식품의 복용 전후에 시력을 측정한 도표,
도 9는 본 발명에 의해 제조된 꿀벌 화분을 이용한 건강식품의 복용 전후에 식전 혈당을 측정한 것이다.
도 10은 본 발명에 의해 제조된 꿀벌 화분을 이용한 건강식품의 복용 전후에 총콜레스테롤 함량을 측정한 도표이다.BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart of a method for manufacturing a health food using bee pollen of the present invention,
FIG. 2 is a graph showing the preference of bee pollen fermentation broth according to fermentation microorganisms,
FIG. 3 is a graph showing the preference of bee pollen fermentation broth according to fermentation time,
FIG. 4 is a graph comparing antimicrobial effects of a healthy food and a propolis using a bee pollen prepared according to the present invention,
FIG. 5 is a graph comparing the electron donating ability of a healthy food with a propolis using honey bee pollen prepared according to the present invention,
FIG. 6 is a graph showing elongation, weight and chest circumference measured before and after taking a healthy food using the bee pollen prepared by the present invention,
FIG. 7 is a graph showing blood pressures measured before and after taking a healthy food using the bee pollen prepared according to the present invention,
FIG. 8 is a graph showing visual acuity measured before and after taking a healthy food using the bee pollen prepared according to the present invention,
FIG. 9 is a graph showing the measurement of pre-eclampsia before and after taking the health food using the bee pollen prepared according to the present invention.
FIG. 10 is a graph showing total cholesterol content measured before and after taking a healthy food using a bee pollen prepared by the present invention. FIG.
이하, 첨부된 도면을 참조하여 본 발명의 꿀벌 화분을 이용한 건강식품 제조방법을 상세히 설명하고자 한다. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Reference will now be made in detail to the preferred embodiments of the present invention, examples of which are illustrated in the accompanying drawings.
도 1에 도시된 바와 같이, 본 발명의 꿀벌 화분을 이용한 건강식품 제조방법은, 꿀벌 화분을 분쇄하여 분말화하는 꿀벌화분 분말화단계와, 상기 꿀벌화분 분말화단계에서 분말화한 벌꿀 화분 8~12 중량부, 정제수 82~90 중량부, 아스퍼질러스 나이거(Aspergillus niger) 1~3 중량부 및 사카로마이세스 세레비지애(Saccharomyces cerevisiae) 1~3 중량부를 혼합하는 제1혼합단계와, 상기 혼합액을 100~200 rpm, 25~35℃에서 10~14시간 발효시켜 제조된 꿀벌 화분 발효액을 여과하여 준비하는 꿀벌화분 발효액 여과단계와, 프로폴리스 원괴를 추출하고 여과하여 준비하는 프로폴리스 추출단계와, 상기 꿀벌화분 발효액 여과단계에서 여과된 꿀벌 화분 발효액 0.7~1.1 중량부, 상기 프로폴리스 추출단계에서 추출된 프로폴리스 1.6~2.0 중량부, 벌꿀 8~12 중량부, 로얄 제리 0.05~0.15 중량부 및 정제수 85~90 중량부를 넣고 혼합하는 제2혼합단계와, 상기 제2혼합단계에서 생성된 혼합액을 여과, 살균 및 포장하는 단계로 구성된다.As shown in FIG. 1, the method of manufacturing a health food using the honey bee pollen of the present invention comprises: a bee pollen powdering step of pulverizing and pulverizing a honey bee pollen; and a honey bee pollen- 12 to 90 parts by weight of purified water, 1 to 3 parts by weight of Aspergillus niger and 1 to 3 parts by weight of Saccharomyces cerevisiae , A bee pollen fermentation filtration step of filtering the honeycomb fermentation broth prepared by fermenting the above mixed solution at 100 to 200 rpm at 25 to 35 ° C for 10 to 14 hours and a step of extracting propolis and filtration to prepare a propolis extraction step 0.7 to 1.1 parts by weight of a bee pollen fermentation filtrate filtered in the bee pollen fermentation broth filtration step, 1.6-2.0 parts by weight of propolis extracted in the propolis extracting step, 8-12 parts by weight of honey, 0.05-0.15 parts by weight of royal jelly, And 85 to 90 parts by weight of purified water are mixed and mixed; and filtering, sterilizing and packing the mixed solution produced in the second mixing step.
본 발명의 꿀벌 화분을 이용한 건강식품 제조방법은 유효성분 중의 하나인 꿀벌 화분 발효액은 꿀벌 화분을 분말화한 후 사카로마이세스 세레비지애(Saccharomyces cerevisiae)와 락토바실러스 플란타룸(Lactobacillus plantarum) 중 어느 하나의 균주와 아스퍼질러스 나이거(Aspergillus niger) 균주를 이용하여 발효하는 단계에 의해 제조된다.The method of manufacturing a health food using the bee pollen according to the present invention is characterized in that a bee pollen fermentation liquid, which is one of effective ingredients, is produced by powdering a bee pollen and cultivating Saccharomyces cerevisiae and Lactobacillus plantarum Or by fermentation using any one of the strains and an Aspergillus niger strain.
꿀벌 화분 발효액은 꿀벌 화분의 선호도가 떨어지는 풍미는 미생물 발효를 통해 극복하여 향미를 개선하거나 증진시킬 수 있다. The honey bee pollen fermented liquid can overcome the preference of honey bee pollen through microbial fermentation to improve or enhance the flavor.
또한, 꿀벌 화분의 두꺼운 세포벽으로 인한 문제는 물리적 파쇄에 이은 효소작용을 통해 달성할 수 있는데, 이때 아스퍼질러스 나이거의 셀룰라아제(cellulase)를 이용할 수 있다. 따라서, 발효 균주로서 셀룰라아제를 생산하는 균주를 이용할 수 있으며, 셀룰라아제를 생산하는 균주이면 특별히 제한되지 않으나, 바람직하게는 아스퍼질러스 속 균주, 더욱 바람직하게는 아스퍼질러스 나이거(Aspergillus niger) 균주를 이용할 수 있다.In addition, the problem caused by the thick cell wall of the bee pollen can be achieved through the enzymatic action following physical breakdown, in which case the cellulase of Aspergillus can be used. Therefore, a strain producing a cellulase can be used as a fermentation strain, and it is not particularly limited as long as it is a strain producing a cellulase. Preferably, however, an Aspergillus strain, more preferably an Aspergillus niger strain, Can be used.
상기 균주 외에 꿀벌 화분 발효액의 선호도를 높이기 위해, 각종 식용가능한 균주를 이용할 수 있으며, 이의 예로는 곰팡이, 효모, 박테리아 등일 수 있으며, 곰팡이는 푸사리움 (Fusarium) 속, 아스퍼질러스 (Aspergillus) 속, 리조푸스 (Rhizopus) 속 등이 있으며, 효모는 사카로마이세스 (Saccharomyces) 속 등이 있으며, 박테리아는 락토바실러스 (Bacillus) 속 등이 있으나, 이에 제한되지 않는다. 상기 식용 가능한 미생물은 구체적으로, 푸사리움 베네나툼 (Fusarium venenatum), 아스퍼질러스 오리제(Aspergillus oryzae), 아스퍼질러스 아와모리 (Aspergillus awamori), 리조푸스 마이크로스포루스(Rhizopus microsporus), 사카로마이세스 세레비지애 (Saccharomyces cerevisiae), 바실러스 메가테리움(Bacillus megaterium), 바실러스 서브틸리스 (Bacillus subtilis), 락토바실러스 플란타룸(Lactobacillus plantarum) 등일 수 있으나, 이에 제한되지 않는다.To increase the affinity of the bee pollen fermentation broth in addition to the above strains, it may be used various edible strain, and examples thereof include and the like fungi, yeasts, bacteria, fungus Fusarium (Fusarium) genus, genus Aspergillus (Aspergillus), separation tank and the like in crispus (Rhizopus), yeast and the like, my process (Saccharomyces) in a Saccharomyces, such as in bacteria include, but are Lactobacillus bacteria (Bacillus), but is not limited thereto. The edible microorganism specifically, Fusarium Venetian natum (Fusarium venenatum), Aspergillus duck claim (Aspergillus oryzae , Aspergillus awamori , Rhizopus microsporus), Celebi as Saccharomyces My jiae access (Saccharomyces but are not limited to, cerevisiae , Bacillus megaterium , Bacillus subtilis , Lactobacillus plantarum , and the like.
바람직하게는 상기 미생물은 락토바실러스 플란타룸(Lactobacillus plantarum) 또는 사카로마이세스 세레비지애 (Saccharomyces cerevisiae)이며, 더욱 바람직하게는 사카로마이세스 세레비지애 (Saccharomyces cerevisiae)이다.Preferably, the microorganism is Lactobacillus Planta room (Lactobacillus plantarum) or saccharide in my process three Levy jiae (Saccharomyces S. cerevisiae , and more preferably Saccharomyces cerevisiae .
상기 미생물을 이용하여 발효하는 방법은 이용하는 미생물에 따라, 발효 조건 및 발효 배지가 달라질 수 있으며, 발효 조건 및 발효 배지는 당업계에 공지된 것을 이용할 수 있다. 예를 들면, 효모의 경우에는 YPD 배지에서 30℃에서 배양할 수 있으며 필요에 따라 호기성 조건을 선택할 수 있다.The fermentation conditions and the fermentation medium may be varied depending on the microorganism to be used, and the fermentation conditions and the fermentation medium may be those known in the art. For example, yeast can be cultured in YPD medium at 30 ° C, and aerobic conditions can be selected as needed.
상기 꿀벌 화분을 분쇄하여 분말화하는 과정은 분쇄기 등을 이용할 수 있다. 또한, 발효 조건은 혼합액을 100~200 rpm, 25~35℃에서 10~14시간 동안 발효시킬 수 있으나, 반드시 이에 한정되는 것은 아니다.The bee pollen may be pulverized and pulverized using a pulverizer or the like. The fermentation conditions may be fermentation of the mixed solution at 100 to 200 rpm at 25 to 35 DEG C for 10 to 14 hours, but not always limited thereto.
상기의 구성에 따른 본 발명의 꿀벌 화분을 이용한 건강식품 제조방법의 동작은 다음과 같다.The operation of the method of manufacturing a health food using the honey bee pollen according to the present invention is as follows.
꿀벌 화분 발효액을 이용한 건강식품을 제조하기 위해 벌꿀, 꿀벌 화분, 프로폴리스 및 로얄 제리는 청정 지역인 충북 괴산군에서 생산된 양봉 산물을 사용하였다.Honey, honey bee pollen, propolis and royal jelly were used in beekeeping products produced in Goesan - gun, Chungbuk province to produce health food using bee pollen fermented broth.
먼저, 도 1에 도시된 바와 같이, 꿀벌 화분 발효액을 제조하기 위해 꿀벌 화분을 분쇄기로 분쇄하여 분말화하고, 분말화한 꿀벌 화분 10 중량부, 정제수 86 중량부, 아스퍼질러스 나이거(Aspergillus niger) 2 중량부, 사카로마이세스 세레비지애(Saccharomyces cerevisiae) 2 중량부를 혼합한 후, 혼합액을 150 rpm, 30℃에서 12시간 발효시킨다.First, as shown in FIG. 1, in order to produce a bee pollen fermentation liquid, bee pollen was pulverized by a pulverizer and pulverized, and 10 parts by weight of pulverized bee pollen, 86 parts by weight of purified water, Aspergillus niger ) And 2 parts by weight of Saccharomyces cerevisiae were mixed, and the mixture was fermented at 150 rpm and 30 ° C for 12 hours.
상기와 같이 제조한 꿀벌 화분 발효액을 여과하여 준비한 후, 프로폴리스 원괴를 추출하고 여과하여 준비하고, 꿀벌 화분 발효액 0.9 중량부, 프로폴리스 1.8 중량부, 벌꿀 10 중량부, 로얄 제리 0.1 중량부 및 정제수 87.2 중량부를 넣고 혼합한 후, 혼합액을 여과, 살균 및 포장한다..The prepared bee pollen fermentation broth was prepared by filtration, and then the propolis powder was extracted and filtered to prepare 0.9 part by weight of fermented bee pollen, 1.8 parts by weight of propolis, 10 parts by weight of honey, 0.1 part by weight of royal jelly, 87.2 parts by weight, and the mixture was filtered, sterilized and packed.
도 2는 발효 미생물에 따른 꿀벌 화분 발효액의 선호도를 조사한 것이다. 그 결과, 아스퍼질러스 나이거(Aspergillus niger)를 단독으로 발효하였을 때보다 락토바실러스 플란타룸(Lactobacillus plantarum)이나 사카로마이세스 세레비지애(Saccharomyces cerevisiae)와 함께 발효하였을 때 선호도가 더 높았고, 아스퍼질러스 나이거(Aspergillus niger)와 락토바실러스 플란타룸(Lactobacillus plantarum)을 함께 발효하였을 때보다 아스퍼질러스 나이거(Aspergillus niger)와 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 함께 발효하였을 때 더 높은 선호도를 나타내었다.FIG. 2 is a graph showing the preference of fermented bee pollen according to fermentation microorganisms. As a result, preference was higher when fermenting with Lactobacillus plantarum or Saccharomyces cerevisiae than when fermenting Aspergillus niger alone, Aspergillus and this (Aspergillus niger) and Lactobacillus Planta Room (Lactobacillus plantarum) than the hayeoteul fermented with Aspergillus or this (Aspergillus niger) and Saccharomyces access to my Celebi jiae (Saccharomyces When S. cerevisiae was fermented together, it showed higher preference.
도 3은 꿀벌 화분 발효액을 제조시 적절한 발효 시간을 결정하기 위해 꿀벌 화분을 6시간, 12시간, 18시간 및 24시간 동안 발효하여 선호도를 조사한 것이다. 그 결과, 12시간 발효한 발효액이 가장 높은 선호도를 나타내어 12시간 동안 발효하는 것이 가장 적절하다고 생각되었다.FIG. 3 shows the preference of bee pollen fermented for 6 hours, 12 hours, 18 hours, and 24 hours in order to determine an appropriate fermentation time in the production of bee pollen fermentation broth. As a result, the fermentation broth fermented for 12 hours showed the highest preference, and fermentation for 12 hours was considered most appropriate.
도 4는 본 발명에 의해 제조된 꿀벌 화분 발효액을 이용한 건강식품과 프로폴리스(물 추출, 에탄올 추출)의 항균 효과를 비교한 것으로, 실험에 사용된 농도는 프로폴리스(물 추출, 에탄올 추출)의 경우 프로폴리스 1%, 본 발명의 꿀벌 화분 발효액을 이용한 건강식품은 50%로 프로폴리스 0.9%가 함유된 샘플을 사용하였다. 항균 효과를 비교하기 위해 대장균의 배양조건은 nutrient broth로 37℃에서 150 rpm으로 12시간 동안 배양하면서 관찰하였다. FIG. 4 is a graph comparing the antimicrobial effect of a healthy food and a propolis (water extraction, ethanol extraction) using a bee pollen fermentation liquid prepared according to the present invention. The concentrations used in the experiment were propolis (water extraction, ethanol extraction) 1% of propolis, and 50% of healthy food using the honey bee pollen fermentation liquid of the present invention, 0.9% propolis was used. To compare the antimicrobial effects, E. coli was cultured in nutrient broth at 37 ° C and 150 rpm for 12 hours.
그 결과, 대장균 배양 6시간까지는 대장균 농도가 모든 샘플에서 처음과 유사한 농도였으나, 6시간 이후에 프로폴리스 물 추출물은 대장균 세포 농도가 다른 샘플에 비해 급격하게 높아졌고, 프로폴리스 에탄올 추출물과 본 발명의 건강식품은 처음과 유사한 농도를 유지하였다.As a result, the E. coli concentration was similar to that of the first sample in all samples up to 6 hours after the coliform culture, but the propolis water extract showed a sharp increase in the E. coli cell concentration after 6 hours and the propolis ethanol extract and the Healthy foods maintained a similar concentration as the first.
따라서, 본 발명의 건강식품은 항균 효과가 있음을 알 수 있다.Therefore, it can be seen that the health food of the present invention has an antibacterial effect.
도 5는 본 발명에 의해 제조된 꿀벌 화분 발효액을 이용한 건강식품과 프로폴리스(물 추출, 에탄올 추출)의 전자공여능(Electron donating ability, EDA) 효과를 추출물의 농도별로 비교한 것이다. FIG. 5 is a graph comparing the electron donating ability (EDA) effect of healthy food and propolis (water extraction, ethanol extraction) using the bee pollen fermentation broth prepared according to the present invention by concentration of the extract.
그 결과, 추출물의 농도가 높아질수록 모든 샘플이 증가하는 경향을 나타내었다. 그 중 본 발명에 의해 제조된 꿀벌 화분 발효액을 이용한 건강식품이 프로폴리스(물 추출, 에탄올 추출)에 비해 더 높은 전자공여능 효과를 나타내었다.As a result, all samples showed an increasing tendency as the concentration of the extract increased. Among them, the health food using the bee pollen fermentation liquid prepared according to the present invention showed higher electron donating effect than propolis (water extraction, ethanol extraction).
도 6은 본 발명에 의해 제조된 꿀벌 화분 발효액을 이용한 건강식품을 복용하기 전과 복용 후를 비교하여 신장, 체중 및 가슴둘레를 측정한 것이다. 도 6에서 14M은 만 14세의 남성이고, 10M은 만 10세의 남성이고, 9M은 만 9세의 남성이고, 8M은 만 8세의 남성이고, 5M은 만 5세의 남성이고, 4F는 만 4세의 여성을 나타낸다.FIG. 6 is a graph comparing the height, weight, and chest circumference of a bee pollen fermentation broth manufactured according to the present invention before and after taking a healthy food. In FIG. 6, 14M is a 14 year old male, 10M is a 10 year old male, 9M is a 9 year old male, 8M is a 8 year old male, 5M is a 5 year old male, Represents a 4 year old woman.
그 결과, 복용 전과 비교하여 복용 후 대부분 신장, 체중, 가슴둘레가 증가하여 본 발명의 건강식품이 성장 촉진 효과가 있는 것으로 나타났다.As a result, it was found that most of the health food of the present invention had growth promoting effect by increasing height, weight, and chest circumference after taking it as compared with before taking.
도 7은 본 발명에 의해 제조된 꿀벌 화분 발효액을 이용한 건강식품을 복용하기 전과 복용 후에 혈압을 측정한 것이다. 그 결과, 복용 전 수축기 혈압 80 mmHg인 사람들이 복용 후 90~104 mmHg의 범위로 나타나 수축기 혈압의 정상 범위인 90~119 mmHg의 범위로 나타났으며, 이완기 혈압이 정상수준보다 낮은 사람들이 본 발명의 건강식품을 복용한 후 이완기 혈압이 높아진 것으로 나타나, 본 발명의 건강식품이 낮은 혈압을 정상 수준으로 올려주는 효과가 있는 것으로 나타났다.7 is a graph showing blood pressures measured before and after taking a healthy food using the bee pollen fermentation broth prepared according to the present invention. As a result, the pre-dose systolic blood pressure of 80 mmHg was in the range of 90 to 104 mmHg after administration and was in the range of 90 to 119 mmHg, which is the normal range of systolic blood pressure. When the diastolic blood pressure was lower than the normal level, The diabetic blood pressure was increased after taking the health food of the present invention, and thus the health food of the present invention showed the effect of raising the low blood pressure to the normal level.
도 8은 본 발명에 의해 제조된 꿀벌 화분 발효액을 이용한 건강식품을 복용하기 전과 복용 후에 시력을 검사한 것으로, 거의 대부분의 사람이 본 발명의 건강식품을 복용하였을 때 복용 전보다 시력이 더 높아지는 것으로 나타나 본 발명의 건강식품이 시력 개선 효과가 있는 것으로 나타났다.FIG. 8 shows the visual acuity of the bee pollen fermentation broth prepared before and after taking the health food prepared according to the present invention. When almost all people took the health food of the present invention, the visual acuity was higher than that before taking it The health food of the present invention has an effect of improving eyesight.
도 9는 본 발명에 의해 제조된 꿀벌 화분 발효액을 이용한 건강식품을 복용하기 전과 복용 후에 식전 혈당을 측정한 것으로, 본 발명의 건강식품을 복용하였을 때 복용 전보다 혈당이 낮아져 본 발명의 건강식품이 혈당 저하 효과가 있는 것으로 나타났다.FIG. 9 is a graph showing the measurement of pre-meal blood glucose levels before and after taking a healthy food using the bee pollen fermentation broth prepared according to the present invention. When the health food of the present invention was used, .
도 10은 본 발명에 의해 제조된 꿀벌 화분 발효액을 이용한 건강식품을 복용하기 전과 복용 후에 총콜레스테롤 수치를 측정한 것으로, 본 발명의 건강식품을 복용하였을 때 복용 전보다 총콜레스테롤 수치가 낮아져 본 발명의 건강식품이 콜레스테롤 저하 효과가 있는 것으로 나타났다.FIG. 10 is a graph showing the total cholesterol levels measured before and after taking a healthy food using the bee pollen fermentation broth prepared according to the present invention. When the health food of the present invention was used, the total cholesterol level was lower than that before taking the health food, Food was found to have a cholesterol-lowering effect.
Claims (1)
상기 꿀벌화분 분말화단계에서 분말화한 벌꿀 화분 8~12 중량부, 정제수 82~90 중량부, 아스퍼질러스 나이거(Aspergillus niger) 1~3 중량부 및 사카로마이세스 세레비지애(Saccharomyces cerevisiae) 1~3 중량부를 혼합하는 제1혼합단계;
상기 혼합액을 100~200 rpm, 25~35℃에서 10~14시간 발효시켜 제조된 꿀벌 화분 발효액을 여과하여 준비하는 꿀벌화분 발효액 여과단계;
프로폴리스 원괴를 추출하고 여과하여 준비하는 프로폴리스 추출단계;
상기 꿀벌화분 발효액 여과단계에서 여과된 꿀벌 화분 발효액 0.7~1.1 중량부, 상기 프로폴리스 추출단계에서 추출된 프로폴리스 1.6~2.0 중량부, 벌꿀 8~12 중량부, 로얄 제리 0.05~0.15 중량부 및 정제수 85~90 중량부를 넣고 혼합하는 제2혼합단계; 및
상기 제2혼합단계에서 생성된 혼합액을 여과, 살균 및 포장하는 단계를 구비한 것을 특징으로 하는 꿀벌 화분을 이용한 건강식품 제조방법.A bee pollen powdering step of pulverizing and pulverizing a bee pollen;
8 to 12 parts by weight of honey powder pollinated in the honey bee pollen pulverization step, 82 to 90 parts by weight of purified water, 1 to 3 parts by weight of Aspergillus niger and 1 to 3 parts by weight of Saccharomyces cerevisiae ) 1 to 3 parts by weight;
Filtering the bee pollen fermentation broth prepared by fermenting the mixed solution at 100 to 200 rpm at 25 to 35 ° C for 10 to 14 hours to filter the bee pollen fermentation broth;
A propolis extraction step in which the propolis raw material is extracted and filtered to prepare;
0.7 to 1.1 parts by weight of a bee pollen fermentation filtrate filtered in the honeycomb fermentation broth filtration step, 1.6 to 2.0 parts by weight of propolis extracted in the propolis extracting step, 8 to 12 parts by weight of honey, 0.05 to 0.15 parts by weight of royal jelly, 85 to 90 parts by weight; And
And a step of filtering, sterilizing and packing the mixed solution produced in the second mixing step.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20220001062U (en) | 2020-11-05 | 2022-05-12 | 황현 | Device for griding and storage honey bee pollen |
| CN115336693A (en) * | 2022-08-25 | 2022-11-15 | 齐鲁工业大学 | Bee pollen fermented sports beverage and preparation method thereof |
| KR102653339B1 (en) | 2022-11-21 | 2024-04-01 | 심영근 | Method for producing a health functional food composition comprising propolis, royal jelly and pollen |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040025226A (en) | 2002-09-18 | 2004-03-24 | 파미셀주식회사 | an antiseptic of containing an extract to pollen-fermentation |
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2017
- 2017-12-13 KR KR1020170170887A patent/KR20190071017A/en not_active Application Discontinuation
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040025226A (en) | 2002-09-18 | 2004-03-24 | 파미셀주식회사 | an antiseptic of containing an extract to pollen-fermentation |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20220001062U (en) | 2020-11-05 | 2022-05-12 | 황현 | Device for griding and storage honey bee pollen |
| CN115336693A (en) * | 2022-08-25 | 2022-11-15 | 齐鲁工业大学 | Bee pollen fermented sports beverage and preparation method thereof |
| KR102653339B1 (en) | 2022-11-21 | 2024-04-01 | 심영근 | Method for producing a health functional food composition comprising propolis, royal jelly and pollen |
| KR20240074622A (en) | 2022-11-21 | 2024-05-28 | 심영근 | Method for producing honey with excellent long-term storage stability containing coated granules |
| KR20240074621A (en) | 2022-11-21 | 2024-05-28 | 심영근 | Method for producing honey comprising propolis, royal jelly and pollen |
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