KR20240074622A - Method for producing honey with excellent long-term storage stability containing coated granules - Google Patents

Abstract

The present invention relates to a method for producing a health functional food composition containing propolis, royal jelly, and pollen. The present invention includes the steps of (1) producing granules containing propolis, royal jelly, pollen, microcrystalline cellulose, lactose, and hydroxypropyl cellulose; (2) sifting the granules through a sieve with a mesh of 710 um or less; (3) coating the granules using a water-soluble ethylcellulose dispersion containing ethylcellulose, oleic acid, ammonium hydroxide, and purified water and ethanol-insoluble alginate; and (4) adding the coated granules to honey.

Description

Method for producing honey with excellent long-term storage stability including coated granules {METHOD FOR PRODUCING HONEY WITH EXCELLENT LONG-TERM STORAGE STABILITY CONTAINING COATED GRANULES}

The present invention relates to a method for producing a health functional food composition containing propolis, royal jelly, and pollen.

Propolis is a substance made by bees by mixing their own saliva and secretions such as enzymes with various saps such as resin extracted from various plants and pollen collected from flowers for their own survival and reproduction. Propolis, also called Russian penicillin or natural penicillin, is used by bees to protect themselves from germs and viruses by applying it to cracks in the hive, and to prevent the invasion of enemies such as wasps and rats. It is used for egg laying and growth, ripening and storage of honey, etc. Maintain suitable habitat. In particular, when the queen bee lays eggs, the worker bees use propolis to disinfect the egg-laying area. Contains alcohol-soluble resins, beeswax, essential oil components, and phenolic compounds.

Meanwhile, royal jelly is provided from the mouths of worker bees as food for the queen bee. Worker bees eat pollen and honey as their staple food, and the digested and absorbed nutrients are transported to the pharyngeal gland on the head through blood vessels, stored as royal jelly, and then supplied to the queen bee. Larvae that receive a concentrated supply of royal jelly develop ovaries that are mature enough to lay eggs and become queen bees.

Royal jelly is a high-protein food that helps supply nutrition, promote and maintain health, and contains moisture (60-70%), protein (9-18%), fat (4-8%), carbohydrate (11-23%), It contains over 40 types of bioactive substances, including vitamins and minerals. Royal jelly strengthens biological resistance, promotes growth, has anticancer effects, has antibacterial effects, and affects the endocrine, circulatory, and hematopoietic organs. In addition, it is effective in maintaining the health of the elderly, loss of appetite in the elderly, and neurotic patients, and is also good for preventing arteriosclerosis because it lowers blood cholesterol levels. In addition, it is helpful for patients with neurological disorders, especially depression, and is also good for patients recovering from surgery. Royal jelly is known to be effective in preventing aging because it contains a large amount of substances similar to the salivary gland hormone parotin. However, royal jelly has a problem with low consumer preference due to its unique fishy taste and aroma.

Bee pollen is a mixture of nectar and saliva when worker bees collect flower nectar and pollen. The worker bees attach it to their hind legs and carry it to the hive to use it as food for bee larvae and queen bees. The pollen contains carbohydrates (25-27%), proteins (23-25%), fats (2%), vitamins, amino acids, minerals and various secondary metabolites. Secondary metabolites include phenolic compounds, anthocyanins, terpenoids, tannins, and polyamines. Many athletes in Europe are taking bee pollen collected with bee pollen long-term as a nutritional supplement to improve athletic performance.

Korean Patent Publication No. 10-2019-0071017

The purpose of the present invention is to provide a method for producing a health functional food composition containing propolis, royal jelly, and pollen.

The present invention also aims to provide a method for producing a health functional food composition that can be stored for a long period of time in honey by coating a composition containing propolis, royal jelly, and pollen.

The present invention includes the steps of (1) producing granules containing propolis, royal jelly, pollen, microcrystalline cellulose, lactose, and hydroxypropyl cellulose; (2) sifting the granules through a sieve with a mesh of 710 um or less; (3) coating the granules using a water-soluble ethylcellulose dispersion containing ethylcellulose, oleic acid, ammonium hydroxide, and purified water and ethanol-insoluble alginate; and (4) adding the coated granules to honey.

In step (1), based on 100% by weight of the granules, the propolis is 1% by weight and less than 5% by weight, the royal jelly is 1% by weight and less than 3.6% by weight, and the pollen is 5% by weight and 28.8% by weight. The microcrystalline cellulose is included in an amount of 40% by weight to less than 70% by weight, the lactose is included in an amount of 20% by weight to less than 30% by weight, and the hydroxypropyl cellulose-LF is included in an amount of 1% by weight to less than 8% by weight.

The weight ratio of the pollen and microcrystalline cellulose may be 1:2 or more and 1:3 or less.

The weight ratio of the microcrystalline cellulose and lactose may be greater than 1:1 and less than 1:3.

The pollen may have a particle size of 300 um or less.

In step (3), the coating rate may be 4% or more and less than 12%.

The hydroxypropyl cellulose may be hydroxypropyl cellulose with a viscosity of 7 to 150 cps at 25°C.

The ethanol-insoluble alginate may be sodium alginate.

According to the present invention, the object is to provide a method for producing a health functional food composition that can be stored for a long period of time in honey by coating a composition containing propolis, royal jelly, and pollen.

Figure 1 is a diagram showing the granules of a composition containing propolis, royal jelly, and pollen according to an embodiment of the present invention and the steps for establishing the same.
Figure 2 is a diagram showing the granules of a composition containing propolis, royal jelly, and pollen according to one embodiment of the present invention and the steps for forming the same.
Figure 3 is a diagram showing the granules of a composition containing propolis, royal jelly, and pollen according to an embodiment of the present invention and the steps for forming the same.
Figure 4 is a diagram showing the steps of preparing and formulating a composition containing propolis, royal jelly, and pollen into 100 g granules according to an embodiment of the present invention.
Figure 5 is a diagram showing the granules of a composition containing propolis, royal jelly, and pollen according to an embodiment of the present invention and the steps for forming the same.
Figure 6 is a diagram testing the enteric effect over time by coating 6% of sugar spheres.
Figure 7 is a diagram testing the enteric effect over time by coating 12% of sugar spheres.
Figure 8 is a diagram testing the enteric effect over time when a composition containing propolis, royal jelly, and pollen according to an embodiment of the present invention is coated and included in honey.
Figure 9 is a diagram testing the enteric effect over time when a composition containing propolis, royal jelly, and pollen was included in honey without coating.
Figure 10 is a diagram testing the long-term storage stability effect when a composition containing propolis, royal jelly, and pollen according to an embodiment of the present invention is coated and included in honey.

The present invention includes the steps of (1) producing granules containing propolis, royal jelly, pollen, microcrystalline cellulose, lactose, and hydroxypropyl cellulose-LF; (2) sifting the granules through a sieve with a mesh of 710 um or less; (3) coating the granules using a water-soluble ethylcellulose dispersion containing ethylcellulose, oleic acid, ammonium hydroxide, and purified water and ethanol-insoluble alginate; and (4) adding the coated granules to honey.

In the present invention, “health functional food” includes food manufactured and processed using raw materials or ingredients with functionality useful to the human body pursuant to Act No. 6727 on Health Functional Food.

Hereinafter, each step will be described in detail.

(1) Preparing granules containing propolis, royal jelly, pollen, microcrystalline cellulose, lactose, and hydroxypropyl cellulose-LF

The propolis is a substance made by bees by mixing their own saliva and secretions such as enzymes with various sap such as resin extracted from various plants and pollen collected from flowers for their own survival and reproduction. It is obtained from nature or is commercially available. You can use what is sold in powder form. The propolis is contained in an amount of 1% by weight or more and less than 5% by weight based on 100% by weight of the granules.

The royal jelly is secreted from the pharyngeal gland on the head of young worker bees, and contains moisture (60-70%), protein (9-18%), fat (4-8%), carbohydrates (11-23%), and vitamins. It contains over 40 types of bioactive substances, including minerals. Those obtained from nature or those sold commercially can be powdered and used. The royal jelly is contained in an amount of 1% by weight or more and less than 3.6% by weight based on 100% by weight of the granules.

The pollen is a mixture of nectar and saliva when worker bees collect flower nectar and pollen, and includes carbohydrates, proteins, fats, vitamins, amino acids, minerals, and various secondary metabolites. You can use those obtained from nature or those sold commercially. The pollen is included in an amount of 5% by weight or more and less than 28.8% by weight based on 100% by weight of the granules. The pollen preferably has a particle diameter of 300 um or less, and if it exceeds 300 um, granules are not formed well when mixed with propolis and royal jelly powder.

The microcrystalline cellulose (CAS RN 9004-34-6; molecular formula (C ₆ H ₁₀ O ₅ ) where _n is approximately 220; molecular weight approximately 36000 g/m) is a white, odorless and tasteless substance composed of porous particles. It is a purified, partially depolymerized cellulose that occurs as a crystalline powder. The microcrystalline cellulose is contained in an amount of 40% by weight or more and less than 70% by weight based on 100% by weight of the granules. The weight ratio of the pollen and microcrystalline cellulose may be 1:2 or more and 1:3 or less, and is preferably 1:3. If the ratio is less than 1:2, granules are produced, but most of the granules are broken during the sizing step, which is not desirable, and if it is more than 1:3, they are not well filtered out in the sizing step.

The lactose is a natural monosaccharide obtained from milk and consists of one galactose and one glucose portion. Lactose occurs as white to off-white crystalline particles or powder. In the present invention, anhydrous lactose (CAS RN 63-42-3; molecular formula C ₁₂ H ₂₂ O ₁₁ ; molecular weight 342.30 g/m), inhaled lactose, lactose monohydrate (CAS RN 5989-81-1; molecular formula C ₁₂ H ₂₂ O) _{11.H 2} O; molecular weight 360.31 g/m), and spray-dried lactose, which is a mixture of amorphous lactose (1:1 mixture of α- and β-lactose) and lactose monohydrate. If lactose is included, granules may be formed well when producing granules weighing 100 g or more, but if lactose is not included, granules may not be formed well when producing granules weighing less than 100 g.

The lactose may be included in an amount of 20% by weight or more and less than 30% by weight based on 100% by weight of the granules. The weight ratio of the microcrystalline cellulose and lactose may be greater than 1:1 and less than 1:3, and is preferably 1:2. Inclusion of lactose reduces granule breakage. If the weight ratio is less than 1:1, granules may not be formed well.

It is preferable to use hydroxypropyl cellulose having a viscosity of 7 to 150 cps at 25°C. Hydroxypropyl cellulose, which has a viscosity of 7 to 150 cps at 25°C, produces granules more uniformly than low-substituted hydroxypropyl cellulose. The hydroxypropyl cellulose may be included in an amount of 1% by weight or more and less than 8% by weight based on 100% by weight of the granules.

(2) sifting the granules through a sieve with a mesh of 710um or less.

The granules are a state in which the mixture prepared in step (1) is agglomerated into lumps with a particle diameter of 1 μm to 10 mm. Since the particle size distribution of granules is uneven, the particle size distribution must be made uniform through the sizing step.

If sizing is performed through a sieve with a mesh exceeding 710um, sizing with an even particle size distribution may not be produced.

(3) Coating the granules using a water-soluble ethylcellulose dispersion containing ethylcellulose, oleic acid, ammonium hydroxide, and purified water and ethanol-insoluble alginate.

The water-soluble ethylcellulose dispersion includes ethylcellulose, oleic acid, ammonium hydroxide, and purified water.

The ethanol-insoluble alginate includes sodium alginate, alginate in other salt forms, alginate in nonionic form, and any combination thereof, and sodium alginate is preferably used. Non-limiting examples of commercially available ethanol insoluble sodium alginate products include PROTANAL® CR 8133 (FMC Corporation), PROTANAL® LFR5/60 (FMC Corporation), and NS ENTERIC® (Colorcon).

The coating rate of the granules is 4% or more and less than 12%, and is preferably 6%. If the content is less than 4%, the enteric properties become poor, and if the content is more than 12%, it disintegrates in the honey and the storage stability of the honey decreases.

The coating rate is calculated as follows:

(4) Adding the coated granules to honey

The coated granules are added to honey to prepare a health functional food composition. For 100 parts by weight of honey, 1 to 30 parts by weight of granules are included. If it exceeds 30 parts by weight, the granules are included in too much and the texture is not smooth.

Example

Next, the present invention will be described in detail through examples. However, the present invention is not limited to these examples.

Selection of ratio of pollen and microcrystalline cellulose and selection of particle size of pollen

Prepare commercially purchased propolis powder and royal jelly powder with a particle size of 100~300um. Filter the pollen powder purchased on the market using a No. 40 sieve (425um sieve size) or a No. 50 sieve (300um), and filter the pollen powder, propolis powder, royal jelly powder, microcrystalline cellulose, and low-substituted hydroxypropyl cellulose. Mix for 3 minutes to prepare 5g of granules. Dry at 40 degrees Celsius for 1 hour, and sieve the granules through a No. 25 sieve (710um sieve size). The results are shown in Table 1 below and Figures 1 and 2.

Ingredient name
(weight%) Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Composition 6 propolis 7.2 5.9 5.0 7.2 5.0 3.9 royal jelly 5.2 4.2 3.6 5.2 3.6 2.8 Flowerpot (No. 40 sieve) 41.2 33.9 28.8 0 0 0 Flowerpot (No. 50 sieve) 0 0 0 41.2 28.8 22.1 MCC 102 41.2 50.8 57.6 41.2 57.6 66.3 L-HPC 5.2 5.1 5.0 5.2 5.0 5.0 Total 100 100 100 100 100 100

As shown in Table 1 and Figures 1 and 2, compositions 1 to 3 formed granules, but most of the granules were broken after sizing, whereas compositions 4 to 6 did not agglomerate well during the granule formation process and the granules were less broken. . In addition, in compositions 3 and 6, where the weight ratio of pollen and microcrystalline cellulose was 1:3, granules were formed well, and the granules were less broken even after sizing.

Selection of HPC

A comparative experiment was conducted to select the type of hydroxypropyl cellulose. Specifically, low-substituted hydroxypropyl cellulose (L-HPC) and hydroxypropyl cellulose (HPC-LF) with a viscosity of 7 to 150 cps at 25°C are compared and the results are shown in Table 2 and Figure 3 below.

Ingredient name
(weight%) Composition 7 Composition 8 propolis 3.9 3.9 royal jelly 2.8 2.8 Flowerpot (No. 50 sieve) 22.1 22.1 microcrystalline cellulose 66.3 66.3 L-HPC 5.0 0 HPC-LF 0 5.0 Total 100 100

As shown in Table 2 and Figure 3, it can be seen that when hydroxypropyl cellulose with a viscosity of 7 to 150 cps at 25°C is used, the granules are less broken even after sizing.

Selection of composition ratio for mass production

When producing 100 g of granules using Composition 6, the granules were not formed, and all of the granules were broken after sizing (see Figure 4). Accordingly, lactose was additionally added to prepare 100g granules. The specific composition ratio is shown in Table 3 below, and the results of composition 10 are shown in Figure 5.

Ingredient name
(weight%) Composition 9 Composition 10 Composition 11 propolis 3.9 3.9 3.9 royal jelly 2.8 2.8 2.8 Flowerpot (No. 50 sieve) 22.1 22.1 22.1 microcrystalline cellulose 33.1 44.2 55.3 lactose 33.1 22.1 11.1 HPC-LF 5.0 5.0 5.0 Total 100 100 100

As shown in Table 3 and Figure 5, when lactose was included, the condition of the granules after sizing was excellent when producing 100 g granules, and in particular, the condition was best when the weight ratio of microcrystalline cellulose and lactose was 2:1. In the case of compositions 9 and 11, some granules broke after sizing.

Coating rate selection

To select the coating rate, an experiment was conducted using sugar spheres (20/25mesh), which exhibit similar behavior to the granules of the present invention. Using a sugar sphere, 40.8 g of water-soluble ethyl cellulose dispersion (surelease) containing ethyl cellulose, oleic acid, ammonium hydroxide, and purified water, and 1.8 g of sodium alginate (NS enteric), a coating rate of 6% was applied using a coating gun. Alternatively, it was coated to make it 12%. Coating conditions are Inlet temperature: 79~75℃, fan flow: 25m ³ /h, flow rate: 1ml/min, atomizing pressure: 2bar, bed temperature 45~50℃.

After weighing 50 mg of the coated sugar shear, it was added to 10 ml of water and honey at pH 1.2, 4.0, and 6.8, and the degree of disintegration was observed while rotating at 5 rpm in a rotary mixer. The results when the coating rate is 6% are shown in Figure 6, and the results when the coating rate is 12% are shown in Figure 7.

As shown in Figures 6 and 7, when the coating rate was 6%, it disintegrated at pH 6.8 after 60 minutes and did not disintegrate in honey. However, when the coating rate was 12%, it did not disintegrate in all water and honey. Therefore, it was confirmed that the storage stability and enteric effect for honey were excellent when the coating rate was 6%.

Enteric properties test of coated granules

The granules of Composition 11 were coated with 40.8 g of a water-soluble ethyl cellulose dispersion (surelease) containing ethyl cellulose, oleic acid, ammonium hydroxide, and purified water and 1.8 g of sodium alginate (NS enteric) at a coating rate of 6%. Coating conditions are Inlet temperature: 79~75℃, fan flow: 25m ³ /h, flow rate: 1ml/min, atomizing pressure: 2bar, bed temperature 45~50℃.

After weighing 50 mg of the coated granules and the uncoated granules of Composition 11, they were added to 10 ml of water and honey at pH 1.2, 4.0, and 6.8, and the degree of disintegration was observed while rotating at 5 rpm in a rotary mixer. The results of the coated granules are shown in Figure 8, and the results of the uncoated granules are shown in Figure 9.

As shown in Figures 8 and 9, it can be seen that the coated granules have excellent enteric properties, but the uncoated granules disintegrate in water of all pHs and have poor enteric properties.

Long-term storage stability experiment on honey

50 mg of the granules of composition 11 coated at a coating rate of 6% and the granules of composition 11 uncoated were weighed in an amount of 50 mg. Add to 10ml of honey and disperse at 5rpm in a rotary mixer. The long-term stability of the granules dispersed in honey was confirmed while stored at room temperature for 1 month. The results are shown in Figure 10.

As shown in Figure 10, the uncoated granules settled and mostly disintegrated after 1 month, while the coated granules did not settle or disintegrate even after 1 month.

Claims (1)

(1) manufacturing granules containing propolis, royal jelly, and pollen;
(2) sifting the granules through a sieve;
(3) coating the granules using a water-soluble ethylcellulose dispersion containing ethylcellulose, oleic acid, ammonium hydroxide, and purified water and sodium alginate; and
(4) adding the coated granules to honey,
A method for producing honey including coated granules, wherein the coating rate in step (3) is 4% or more and less than 12%.