KR20190063025A - Wet Tissue composition and wet Tissue containing it - Google Patents

Abstract

A wet wipe composition of the present invention and a wet wipe containing the same can replace or minimize the amount of a chemical preservative, minimizes skin side effects such as skin irritation, allergic symptoms and the like, and has a sterilizing effect that can kill pathogenic bacteria as well as a function as a preservative by having an antimicrobial activity that does not allow microorganisms to proliferate, thereby having an effect of controlling skin flora.

Description

[0001] The present invention relates to a wet tissue composition and a wet tissue including the wet tissue composition,

The present invention relates to a wet tissue composition and a wet tissue including the same, and more particularly, to an antimicrobial active wet tissue composition comprising a natural antibacterial extract which is harmless to human body and has an excellent antibacterial effect as a main component for antibacterial activity, .

Wet tissue is a one-time cleaning product that absorbs a substance that imparts detergency to a substrate such as a cotton or nonwoven fabric. There are a wide variety of uses for it. For example, it has various uses such as use for discharging infant feces, cleaning for public use in public places, cleaning use, cleaning use of industrial products such as device cleaning Products. In recent years, various functional wipes have been developed not only for cleaning use but also for adding functional ingredients according to the purpose of use.

The main use of wet tissues is to use the product in direct contact with the human body. Therefore, in the case of products intended for human body cleaning in Korea, the product is classified as a cosmetic product in 2015 due to the safety problem and is applied to the safety standards of the Food and Drug Administration In fact.

Since the wet tissue is manufactured and packaged in a state in which the liquid composition is impregnated on the substrate such as the nonwoven fabric, the new product before use has basically good conditions for the microbes to proliferate. In the use process, The contamination by the microorganisms is more easily proceeded.

Therefore, a preservative component capable of inhibiting the microbial growth of the wet tissue composition used for impregnating the substrate is an indispensable element. In general, as a preservative component used in a wet tissue composition, methylparaben, butylparaben, benzalkonium chloride, benzoic acid, sorbic acid, ethylparaben, ethylparaben, propylparaben, 2- (4-thiazolyl) -benzimidazole, 3-iodo-2-propynyl bytyl carbamate, , And paraoxybenzoic acid compounds have been used. Such preservatives can cause seizures, erythema and other skin irritation of the skin or can cause direct problems. In addition, the preservatives do not show immediate toxicity, but they contain potential risks such as environmental hormones. In fact, there are many cases in which the incidents that cause such problems are reported recently through the media and cause a great deal of social repercussions.

Therefore, the safety of the wipes that can be directly used on the skin of the human body is more important than the human body. In recent years, studies on harmless and safer preservative ingredients have been intensively studied.

The preservative used in the wet tissue composition is not only a function of suppressing bacterial proliferation but also an object of cleaning a contaminated site, and thus it is possible to remove foreign substances and pathogenic microorganisms that remain in the cleansing site, that is, skin, A sterilization function that can kill pathogens is also required.

Particularly, in the case of infants whose wipes are mainly used for defecation treatment, the resident flora existing on the skin due to the physical skin irritation due to continuous friction and the hydration of the skin due to the moist environment due to the use of the diaper, And is susceptible to second-round infections. Therefore, the infant wipes are required to have a function of controlling the microorganisms in order to prevent rashes, red spots, and skin inflammation which may be caused or exacerbated by microorganisms.

Recently, wet tissue products have been approved by the Food and Drug Administration and contain benzoic acid or humectant used as a food preservative, or 1,2-hexanediol as a side effect system. These components have been proven to have safety as well as performance as a preservative, but the demand of consumers who prefer natural ingredients is rapidly increasing, so that it is urgently required to develop a naturally occurring antimicrobial substance.

Korean Patent Laid-Open Publication No. 10-2017-0105182 (2017.09.19)

It is an object of the present invention to provide an antimicrobial agent capable of replacing or minimizing the use of a chemical preservative and minimizing skin side effects such as skin irritation and allergic symptoms while retaining antimicrobial activity against proliferation of microorganisms, The present invention is to provide a wet tissue composition and a wet tissue including the same, which can sterilize the skin and control the phase sterilization of the skin.

An antimicrobial active agent comprising any one or more selected from the group consisting of gold extract, jasmine extract, ginger extract, chamomile extract and Karenlaura extract; A skin remedy comprising any one or two or more selected from gold particles, honey extract, Centella asiatica extract, Horsetail extract, Rosemary extract and Panthenol; A preservative comprising one or more selected from linoleamidopropyl-diimonium chloride phosphate, 1,2-hexanediol, hydroxyacetophenone and sodium benzoate; And a solvent comprising one or both selected from carbonated water and water.

In one example of the present invention, the antimicrobial active agent may include a jasmine extract.

In one example of the present invention, the skin improving agent may include gold particles.

In one example of the present invention, the antimicrobial active agent may include a gold extract, a jasmine extract, a ginger extract, a chamomile extract, and a calendula extract. The skin remedy may include gold particles, honey extract, Extracts, and panthenol, and the solvent may include carbonated water.

In one example of the present invention, the wet tissue composition may contain 0.01 to 10% by weight of an antimicrobial active agent, 0.01 to 10% by weight of a skin improving agent, 0.001 to 5% by weight of a preservative, and a residual amount of a solvent.

In one example of the present invention, the extract may be a supernatant obtained by extracting the extract material with an ethanol aqueous solution and removing the precipitate by centrifugation, independently of each other.

The wet tissue according to the present invention includes the wet tissue composition. Specifically, the wet tissue may include a substrate impregnated with the wet tissue composition.

The wet tissue composition of the present invention and the wet tissue including the same can minimize the side effects such as skin irritation and allergic symptoms while minimizing the use of chemical preservatives or replacing chemical preservatives. It has a sterilizing effect that can kill pathogenic bacteria as well as a function, so that the phase sterilization of the skin can be controlled.

Even if the effects are not explicitly mentioned in the present invention, the effects described in the specification anticipated by the technical features of the present invention and their inherent effects are treated as described in the specification of the present invention.

Fig. 1 shows antibacterial activity against Gram-positive bacteria of each extract prepared in Preparation Examples 1 to 5.
2 shows antibacterial activity against Gram-negative bacteria of the extracts prepared in Preparation Examples 1 to 5.
FIG. 3 shows the antimicrobial activity of the extracts prepared in Preparation Examples 1 to 5 at 10% and 30% concentration and time of extracts of Gram-positive bacteria.
FIG. 4 shows the antimicrobial activity of 10% and 30% of extracts of Gram-negative bacteria of each extract prepared in Preparation Examples 1 to 5 at various concentrations and time.
5 shows the rate of antimicrobial action of the jasmine extract of Preparation Example 2 over time for Gram-positive bacteria.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a wet tissue composition and a wet tissue including the same according to the present invention will be described in detail with reference to the accompanying drawings.

The drawings described in the present invention are provided by way of example so that a person skilled in the art can sufficiently convey the idea of the present invention. Therefore, the present invention is not limited to the illustrated drawings, but may be embodied in other forms, and the drawings may be exaggerated in order to clarify the spirit of the present invention.

The technical and scientific terms used in the present invention will be understood by those of ordinary skill in the art without departing from the scope of the present invention. A description of known functions and configurations that may unnecessarily obscure the description of the present invention will be omitted.

The singular forms of the terms used in the present invention can be interpreted to include plural forms unless otherwise indicated.

 Unless specifically stated in the present invention, the unit of% used unclearly means% by weight.

An antimicrobial active agent comprising any one or more selected from the group consisting of gold extract, jasmine extract, ginger extract, chamomile extract and Karenlaura extract; A skin remedy comprising any one or two or more selected from gold particles, honey extract, Centella asiatica extract, Horsetail extract, Rosemary extract and Panthenol; Selected from Linoleamidopropyl PG-Dimonium Chloride Phosphate, 1,2-hexanediol, Hydroxyacetophenone, and Sodium benzoate. A preservative comprising any one or more of the foregoing; And a solvent comprising one or both selected from carbonated water and water.

The golden extract means an extract of gold (Scutellaria radix). Gold is used as a root of Scutellaria baicalensis GEORGI, a herbaceous perennial plant belonging to the family Lamiaceae, and has been used for the purpose of chewing and deciphering in a herb. Most of the major active substances in gold are known as flavonoid compounds.

In the above-mentioned jasmine extract, jasmine is a generic term for the plants belonging to the genus Aspergillus in the dicotyledonous spruce, and the spice name taken from the flower of this genus.

As a preferred example, the jasmine extract of the above antimicrobial active agent is superior to the antimicrobial active ingredients having different antimicrobial activity, and the antimicrobial active agent has an improved antimicrobial activity even when used together with other antimicrobial active agents, It can be implemented and contains jasmine extract. In general, the extracts containing the antimicrobial component, when used singly, have excellent antimicrobial properties, but when used in combination with other antimicrobial extracts, the improvement of the antibacterial property is substantially absent or rather reduced. However, in the present invention, when one or more antimicrobial extracts selected from among gold extract, gingkohee extract, chamomile extract, and Karenlaura extract are mixed with jasmine extract, the antibacterial properties of jasmine extract Can be implemented. In one preferred embodiment, the antimicrobial active agent may include a gold extract, a jasmine extract, a ginger extract, a chamomile extract, and a Karenlaura extract. However, this is a preferred example, and the present invention is not limited thereto.

The gingkohwha extract means an extract of Lonicerae Flos, and the gingkohwa is a flower bud or a flower bud of Lonicera japonica Thunberg belonging to Caprifoliaceae.

The above-mentioned chamomile extract means an extract of Matmaria chamomilia, and Chamomile is a mums and a perennial plant originating in Europe and India.

The Karendura extract is an extract of Calendula officinalis, Calendula is a marigold or calendula, and in the West it is an annual plant of Asteraceae called Marigold.

In a preferred embodiment of the present invention, the gold particles in the skin improving agent not only help to improve the skin but also improve the washing power and further enhance the antibacterial activity of the antibacterial active agent. The average particle diameter of the gold particles may be as long as the wettable composition can be impregnated into the substrate and can be used for cleaning the human body. Specifically, 50 nm to 10 탆 may be exemplified, but the present invention is not limited thereto. The shape of the gold particles may be varied, but preferably the spherical or elliptical shape may reduce the friction due to skin contact.

In a more preferred example, when the wipes composition according to the present invention contains a jasmine extract and gold particles, the antibacterial activity and cleaning effect can be remarkably improved, and furthermore, when the watersoluble composition further contains carbonated water, the antibacterial activity and cleaning effect are further improved .

The solvent may be water alone, but it may be carbonated water, or may be mixed with carbonated water and water. When carbonated water is used as the solvent, a cooling sensation can be provided, and the cleaning effect can be further improved. However, this is a preferred example, and the present invention is not limited thereto.

The carbonated water may be an aqueous solution in which carbonic acid (H ₂ CO ₃ ) and / or carbon dioxide (CO ₂ ) are dissolved or contained. Generally, carbon dioxide reacts with water to produce carbonic acid, and the reaction can proceed reversely with reversible reaction, so that carbon dioxide and carbon are equilibrium and exist in the aqueous solution. Therefore, the aqueous solution becomes slightly acidic by the production of carbonic acid, so that the degree of the content of carbonic acid can be controlled by adjusting the pH. Specifically, the pH of the carbonated water is not limited within a range that can achieve the object of the present invention, but it may be 5.5 to 6.7. As a specific example, the above-mentioned carbonated water is a preferred example of the purified water having the above-mentioned pH range and containing various minerals. The purified mineral water is also called, for example, primed carbonated water and contains various minerals. As the components of the present invention and their components are bonded and applied to tissues, the absorption rate of the antimicrobial extract to the substrate can be further improved. In addition, the skin cosmetic effect by the mineral ingredient can be further improved. Further, the detergency against human body such as sweat, foreign matter and the like can be further improved.

The antimicrobial active agent may be contained in an amount of 0.01 to 10% by weight based on the total weight of the wet tissue composition. When the jasmine extract and the other antimicrobial active ingredient are included in the antimicrobial active ingredient, the antimicrobial active ingredients may be included independently in an amount of 50 to 150 parts by weight based on 100 parts by weight of the jasmine extract. However, this is a preferred example, and the present invention is not limited thereto.

The skin improving agent may be included in an amount of 0.01 to 10% by weight based on the total weight of the wet tissue composition. When the gold particles of the skin remover component and the other skin remedies component are included, the skin remover components may be included independently from 100 to 2,000 parts by weight based on 1 part by weight of the gold particles. However, this is a preferred example, and the present invention is not limited thereto.

The preservative may be contained in an amount of 0.001 to 5% by weight, preferably 0.001 to 3% by weight, more preferably 0.001 to 1% by weight based on the total weight of the wet tissue composition. However, this is a preferred example, and the present invention is not limited thereto.

The solvent may be included as a residual amount such that the total weight of the wet tissue composition is 100% by weight. If the solvent contains both super-fine carbonated water and water, the super-fine carbonated water may be included in an amount of 30 to 300 parts by weight based on 100 parts by weight of water. However, it should be understood that the present invention is not limited thereto.

The 'extract' referred to in the present invention may mean that the extract is extracted by a known extraction method, and the specific ingredients and the content contained in the extract are not limited as long as the corresponding raw materials are extracted. Specifically, the extract extracted from the raw material may be one having a solid content of 0.1 to 20% by weight or a concentrate, and may be extracted with an extraction solvent containing water, ethanol or ethanol aqueous solution. The extraction method is not particularly limited, and may be, for example, an extract obtained by extracting the extractive material as an extraction solvent at 20 to 40 ° C for 2 to 24 hours. Further, an extract which is a supernatant obtained by removing the precipitate by centrifuging the above extract may be more preferable. Further, the extract may be concentrated and dried, the extraction solvent may be diluted and centrifuged again to remove the precipitate, and the extract which is a supernatant thereof may be more preferable. When the extract prepared by this method is used, more remarkable antimicrobial activity characteristics can be realized, but this is a preferred example, but the present invention is not limited thereto.

The wet tissue according to the present invention includes the wet tissue composition. Specifically, the wet tissue may include a substrate impregnated with the wet tissue composition. The base material may be any material that can be used for wet tissue used for cleaning the face, cleaning the skin, cleaning and removing make-up, and may be a non-woven fabric, a chemical fiber, a paper sheet, And preferably a cellulose-based fabric, as described above. Such a cellulose-based fabric is preferable in terms of minimizing the irritation of the skin and also having excellent contact with the skin. However, the present invention is not limited thereto, and this description is well known in the art and is not limited.

EXAMPLES Hereinafter, the present invention will be described in detail with reference to Examples. However, the present invention is described in more detail with reference to the following Examples. However, the scope of the present invention is not limited by the following Examples.

[Production Example 1]

10 g of dried gold ( Scutellaria baicalensis Georgi ) powder was mixed with 100 ml of a 95% by weight aqueous ethanol solution and stirred at room temperature for 6 hours to prepare a mixture of extracted active ingredients in gold. The mixture was then centrifuged at 10,000 rpm for 10 minutes using a centrifuge, the precipitate was removed, and the supernatant was concentrated and dried with a vacuum concentrator. 10 ml of sterilized water was added to the dried and dried concentrate, and the undissolved precipitate was separated and removed by centrifugation at 10,000 rpm for 10 minutes using a centrifuge to prepare a gold extract as a supernatant thereof.

[Production Example 2]

The jasmine extract was prepared in the same manner as in Preparation Example 1, except that dried jasmine powder was used in place of the dried golden powder in Preparation Example 1.

[Production Example 3]

A silver microsphere extract was prepared in the same manner as in Preparation Example 1, except that the dried gold silver powder was used in place of the dried gold powder in Preparation Example 1.

[Production Example 4]

A chamomile extract was prepared in the same manner as in Preparation Example 1, except that dried chamomile powder was used in place of the dried golden powder in Preparation Example 1.

[Production Example 5]

A Karenlaura extract was prepared in the same manner as in Preparation Example 1, except that dried Karenlaura powder was used in place of the dried golden powder in Production Example 1.

[Experimental Example 1]

To evaluate the antimicrobial activity of each of the extracts prepared in Preparation Examples 1 to 5, antimicrobial activity against Gram negative bacteria and Gram positive bacteria was evaluated. Teicoplanin, a commercial antibiotic, was used as a positive control, and sterilized third distilled water was used as a negative control.

The antimicrobial activity of each extract was evaluated by a radial diffusion assay and then tested for the antimicrobial activity by a colony count assay.

The bacterium used for the test was as shown in Table 1 and the bacteria were cultured in a 50 ml Mueller-Hilton Broth (MHB: Difco, USA) or Tryptic Soy Broth (TSB: Difco, USA) For 18 hours to a stationary phase and then incubated for 2 hours and 30 minutes to reach a logarithmic phase under the same conditions.

Scientific name (classification number) Place of sale Remarks Staphylococcus aureus (KCTC 1621) KCTC (Life Resource Center) Gram positive Escherichia coli (KCTC 1039) KCTC (Life Resource Center) Gram negative

Radical diffusion method

Antimicrobial activities of these extracts of Preparation Examples 1 to 5 were tested by radial diffusion assay against their gram-positive strains Staphylococcus aureus (KCTC 1621) and gram negative strain Escherichia coli (KCTC 1039) Respectively. The test substance was prepared by diluting the stock solution (100%), 50%, 25%, 12.5%, 6.25% diluted with sterilized water and measuring the size of the clear zone indicating the growth inhibition by the test substance. Respectively.

Specifically, a test substance solution diluted with sterilized distilled water was prepared for each of the extracts prepared in Preparation Examples 1 to 5 as a test substance to be tested for antibacterial activity, as described above. After that, underlay agar (9% 100 mM NaP buffer pH 7.4, 1% 100 mM sodium citrate buffer pH 7.4, 1% low EEO agarose, 0.03% tryptic soy broth containing 4 × 10 ⁶ CFU / A hole with a diameter of 3 mm was made in a powder plate, and 5 μl of each sample was injected. After addition of nutrient-rich overlay agar (1% agarose, 60 mg / ml TSB powder) was added to the underlay agar plate, which was incubated at 37 ° C for 3 hours, and incubated overnight at 37 ° C.

After the culture, the growth of the test bacteria was stopped with an aqueous acetic acid solution having a concentration of 5% by weight, and the size of the clear zone indicating the degree of inhibition of bacterial growth was measured to confirm the degree of antimicrobial activity. In this case, the diameter size except for 3 mm hole for sample injection in clear zone is expressed in units, and 0.1 mm of clear zone corresponds to 1 unit. Ceftriaxone and Teicoplanin used as positive control were also used at concentrations of 1 ㎍ / ㎖, 0.5 ㎍ / ㎖ and 0.25 ㎍ / ㎖.

As a result, the antimicrobial activity against Gram-positive bacteria in each of the extracts is shown in Fig. 1, and Fig. 1 shows a comparison of the anti-bacterial activity against the Gram positive bacteria (S. aureus) Respectively. The antibacterial activity was shown in all cases of Preparation Examples 1 to 5, and the gold extract of Preparation Example 1 and the gold eutectic extract of Preparation Example 3 showed a high level of antibacterial activity. In particular, the jasmine extract of Preparation Example 2 showed the highest antimicrobial activity of 54 units, indicating that the antimicrobial activity was excellent even at a low content.

The antimicrobial activity against Gram-negative bacteria in each extract is shown in Fig. The Gram-negative bacterium exhibited a concentration-dependent antimicrobial activity similar to that of the Gram-positive bacteria, and the jasmine extract showed the highest antibacterial effect of 69 units against E. coli.

Colony counting method

Antimicrobial activity of the gram-positive Staphylococcus aureus (KCTC 1621) and the gram negative strain Escherichia coli (KCTC 1039) was tested using the extracts of Preparation Examples 1 to 5 as test substances. Test bacteria were prepared at a concentration of 2 × 10 ⁵ CFU / ml, and the extracts diluted to 60% and 20% were mixed in the same volume as the solution containing the bacteria. Finally, the concentration of the test substance was 10% 30%. ≪ / RTI > The mixture was allowed to react at 37 ° C. After 10, 20, and 40 minutes, a portion (10 μl) of the reaction solution was aliquoted and the aliquot was diluted or spun onto a plate medium as the raw solution. The plated plate media counted colonies formed after one day of culture at 37 ° C.

Specifically, 100 μl of the prepared test substance solution was added to 10 mM NaP buffer containing the same amount of logarithmic phase bacteria calculated to 2 × 10 ⁶ CFU / ml, and the mixture was added at 37 ° C. After incubation for 1 hour, 10 μl of the mixed solution was aliquoted, and the resultant was diluted or applied to a 1.5% Bacto-agar (Difco, Detroit, USA) at pH 7.4 as it was, and then cultured at 37 ° C or 30 ° C for one day. Were counted to confirm their activity. Ceftriaxone and Teicoplanin, both used as positive control, were also prepared at a concentration of 200 μg / ml.

The antimicrobial activity according to the concentrations of 10% and 30% of each extract against S. aureus is shown in FIG. The test substance showed significant antibacterial activity against Gram positive bacteria used in the test. Compared with the case of the chemomail extract and the Karen dura extract, the golden extract showed excellent antibacterial activity at 30% concentration, reducing the test strain from 10 ⁶ CFU / ml to 10 ³ CFU / ml. Especially, the extract of jasmine showed a very strong activity to completely control the test strain even at the concentration of 10%.

The antimicrobial activity at 10% and 30% concentration of each extract against Gram negative strain ( E. coli ) is shown in FIG. As a result of the antibacterial activity test of each extract against Gram negative bacteria, all of the tested extracts showed significant antibacterial activity. In particular, the extracts of jasmine and chamomile showed a strong anti-bacterial activity which decreased to less than 10 ³ CFU / And exhibited bacterial activity.

As described above, it can be seen that the jasmine extract has the strongest antimicrobial activity in both Gram-positive bacteria and Gram-negative bacteria. In order to confirm the killing kinetics, the antimicrobial activity of Teicoplanin at a concentration of 10 μg / ㎖, which was used as a test substance at a concentration of 30% and the control group, The identified colonies were identified by Gram-positive bacteria (S. aureus). The test method was almost the same as the previous test, but the reaction time was shortened to 1 minute, 5 minutes and 10 minutes.

As a result, it can be confirmed that the action time is relatively fast even in a relatively short time, as shown in the action rate graph of the jasmine extract against gram-positive bacteria shown in FIG. Jasmine extract showed activity from 1 minute after the shortest time of reaction in this test. After 5 minutes of reaction time, less than 10 ³ CFU / ㎖ and after 20 minutes, it decreased to less than 10 ² CFU / ㎖ Strong activity. On the other hand, Teicoplanin, a commercial antibiotic used as a control, started to show activity late after 10 minutes.

Teicoplanin, a commercial antibiotic, controls bacteria by inhibiting glycopeptide polymerization, which is involved in bacterial cell wall synthesis. Therefore, antibacterial action time of teicoplanin is related to bacterial growth rate. On the contrary, it is indirectly known that the activity of jasmine extract starts to show its activity even in a very short time of 1 minute because it contains a component that acts directly on bacteria to kill it.

In the antimicrobial activity test, the bacteria are in an excess amount (10 ⁵ to 10 ⁶ CFU / ml), and the test substances under the condition are controlled to a low level of 10 ⁴ CFU / ml and a high level of 10 ² CFU / This is a high antimicrobial activity effect which reduces more than 99% of bacteria in the whole test organism. Therefore, antimicrobial activity can be anticipated even if these extracts are used at a very low content, and the effect of jasmine extract is expected to be very high.

The components of Table 2 below were thoroughly mixed and dispersed in the stated weight percent composition to produce a wet tissue composition. At this time, the antimicrobial active agents, gold extract, jasmine extract, ginger extract, chamomile extract and calendula extract were used as the extracts prepared in Preparation Examples 1 to 5, respectively.

The prepared wet tissue composition was impregnated in a cotton tissue for 1 hour and then semi-dried at room temperature for 3 hours to prepare a sports wet tissue.

ingredient Example (% by weight) One 2 3 Antimicrobial activator Golden extract 0.05 0.50 1.00 Jasmine extract 0.05 0.50 1.00 Gold Euphoria extract 0.05 0.50 1.00 Chamomile extract 0.05 0.50 1.00 Karen Dura extract 0.05 0.50 1.00 Skin conditioner Honey extract 0.05 0.50 1.00 Panthenol 0.05 0.50 1.00 Centipede extract 0.05 0.50 1.00 Horsetail extract 0.05 0.50 1.00 Rosemary extract 0.05 0.50 1.00 gold 0.0001 0.001 0.02 Preservative 1,2-hexanediol 0.01 Hydroxyacetophenone 0.02 Linoleamidopropyl < RTI ID = 0.0 > diimonium < / RTI > chloride phosphate 0.05 Sodium benzoate 0.05 menstruum Super carbonate water 10.00 Purified water Balance Sum 100.00

[Experimental Example 2]

Primary stimulation evaluation test for human skin

The evaluation of the primary irritation response to the human skin was carried out by the single - seal test method. The human skin patch test is basically a test to judge whether or not dermatitis occurs when the skin external agent is brought into contact with the skin. The skin test is performed on the subject's arm or the like using a patch test patch to evaluate skin irritation and sensitization It is judged by evaluation. The test is performed on subjects with normal skin, and the experts evaluate the reactions of the test results such as erythema, edema, swelling, and palsy to judge the safety of the external preparation for skin.

As a specific test method, 32 healthy subjects without skin disease were enrolled in this study. The mean age was 37.75 years (± 5.87 years). A special patch (IQ series / IQ Ultra Chamber) was used to evaluate skin primary response.

The wet tissue composition prepared in Examples 1 to 3 was dropped into a patch (5 mm x 5 mm) in an amount of 20 to 25 μl as a test product, and the wet tissue composition was adhered to the test site. 24 hours after the patch attachment, the patch was removed, and the test site was visually evaluated by a specialist for 30 minutes to 1 hour after the patch was removed, 24 hours after the patch was removed, and 48 hours after the patch was removed.

As a concrete evaluation method, the evaluation criteria of the stimulation reaction was based on the evaluation method designed by Frosch & Kligman using the reading standard of the International Contact Dermatological Research Committee (ICDRG), and the evaluation criteria are shown in Table 3 below. To ensure the objectivity of the visual evaluation, the degree of skin irritation was determined with reference to the photographs shown in the reference (S. M. An et al. Int J Cosmet Sci., 2014 36: 62-67).

[Table 3]

After the evaluation according to the above evaluation criteria, the average skin irritation index was calculated according to the following formula in Table 4, and the skin irritation intensity was evaluated according to the judgment criteria.

[Table 4]

As a result, as shown in Table 5 below, it can be confirmed that there is practically no possibility of stimulation in real life.

[Table 5]

Claims (7)

An antimicrobial active agent comprising any one or two or more selected from the group consisting of gold extract, jasmine extract, ginger europaea extract, chamomile extract and Karenlaura extract;
A skin remedy comprising any one or two or more selected from gold particles, honey extract, Centella asiatica extract, Horsetail extract, Rosemary extract and Panthenol;
A preservative comprising one or more selected from linoleamidopropyl-diimonium chloride phosphate, 1,2-hexanediol, hydroxyacetophenone and sodium benzoate; And
A solvent containing any one or two selected from carbonated water and water;
≪ / RTI > The method according to claim 1,
Wherein the antimicrobial active agent comprises a jasmine extract. 3. The method of claim 2,
Wherein the skin improving agent comprises gold particles. The method according to claim 1,
The antimicrobial active agent includes a golden extract, a jasmine extract, a ginger extract, a chamomile extract, and a calendula extract,
The skin improving agent includes gold particles, honey extract, centilla sp. Extract, horsetail extract, rosemary extract and panthenol,
Wherein the solvent comprises carbonic acid water. The method according to claim 1,
Wherein the wet tissue composition comprises 0.01 to 10% by weight of an antimicrobial active agent, 0.01 to 10% by weight of a skin improving agent, 0.001 to 5% by weight of a preservative, and a residual amount of a solvent. The method according to claim 1,
Wherein the extract is a supernatant obtained by extracting the extract material with an ethanol aqueous solution and removing the precipitate by centrifugation independently of each other. A wet tissue impregnated with the wet tissue composition of any one of claims 1 to 6.

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