KR20190056328A - Production method of mushroom using mushroom cultivation bag with micropore - Google Patents

Abstract

As described in the present invention, when mushrooms are cultivated by forming micropores on a plastic sheet by laser processing without a filter or cotton, the costs can be reduced while the productivity is improved, thereby reducing the human labour and securing the cost-effectiveness. Further, the plastic sheet provided with the micropores formed by laser processing may secure sufficient air ventilation when cultivating various mushrooms such as button mushrooms, oyster mushrooms, and shiitake mushrooms, as compared to when a regular plastic sheet is used, and thus may assist in promoting and invigorating the growth of culture. Further, the plastic sheet of which diameters of the micropores range from 5 to 300 micrometers (μm) and daily air ventilation amount range from 20,000-2,000,000 cc/m^2 may shorten the cultivation period and reduce the pollution.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a mushroom-containing mushroom culture bag,

The present invention relates to a method for producing mushroom using a bag for culturing mushrooms having fine pores.

Shiitake mushrooms have traditionally been used to grow hardwoods by drilling holes in hardwood logs and inoculating them with mushroom seeds. In recent years, in order to increase mushroom production efficiency and reduce labor force, sawdust media, And cultivation methods in which they are cultivated in the market are popularized and used.

In the early stage of oyster mushroom cultivation, it was mainly cultivated as manpower using natural mushroom grower, wood and rice straw, etc. However, this cultivation was highly efficient, but required a lot of manpower and was seasoned . In order to improve this, an automatic cultivation method has been developed in which various media materials are put into a bottle or bag of a certain size with an automated machine, and the mycelium is harvested by fruiting body.

The above-mentioned sowing cultivation method can automate and mechanize the work in all the processes, so that manpower can be reduced. In case of small scale, it can be cultivated by self-labor power, and it is possible to produce a mechanical standard product. In addition, it is possible to regulate supply and demand of mushroom through planned production because it can cultivate all year round by optimizing temperature and humidity in cultivation company. However, the costs such as facility cost and machine installation cost for cultivation are much higher than those for cultivation, and the quality tends to be slightly lower than that produced by the cultivation of bacteria. Glass bottles are also excellent in heat resistance, which is advantageous for disinfection of the culture medium. However, since the glass bottle is relatively easily broken due to a temperature difference or an external impact, it is difficult to handle due to its heavy weight and its structure can not be folded or overlapped Which is disadvantageous in terms of movement and storage, and the manufacturing cost is high.

In recent years, bag cultivation using mushrooms has been widely used. However, in order to cultivate the mushroom by using the bag, it is necessary to use a cotton or filter to attach the medium material to the bag, sterilize it, inoculate the mushroom, and inhale it for breathing.

To form a vent at the end of the bag, a pipe of about 5 cm in length may be bundled with a cotton stopper, or a special paper filter may be inserted into the polypropylene cap to seal it. Alternatively, a filter is placed next to the bag. However, when using cotton, the work is inconvenient and requires a lot of manpower. In addition, the use of the bag for filter attachment is costly and relatively expensive.

Thus, the present invention aims to develop a method capable of respiration of mushroom bacteria without sticking cotton or filter when cultivating mushrooms.

Registration Utility Model No. 20-0474879

The object of the present invention is to provide a mushroom culture bag capable of breathing with mushroom strains by forming laser micro pores in a bag so that mushroom cultivation is possible without additional cotton or filter when cultivating mushrooms, And to provide a method for producing mushrooms.

In order to accomplish the above object, the present invention provides a method for preparing a mushroom culture bag, comprising the steps of: preparing a bag for cultivation of mushrooms having micropores formed by laser treatment and having an open top;

A second step of putting the medium into the bag, followed by sterilization and cooling;

3) inoculating the mushroom seeds after the cooling;

After the inoculation, the top of the encapsulation front and the top of the back bottom are combined and then sealed by compression heating;

A step 5) of culturing the inoculated mushroom seeds after the above-mentioned sealing step to produce a mycelium; And

Culturing the cultured mycelium and cultivating it by browning.

A method for producing a mushroom using a bag for culturing a mushroom having fine pores is provided.

The method for producing a mushroom using the bag for culturing the micro-pores of the present invention includes a step of preparing a bag for cultivation of mushrooms having micropores formed by laser treatment and having an open top.

Fig. 1 is a perspective view of a mushroom culture bag of the present invention, and Fig. 2 is a perspective view showing another embodiment of the present invention.

In the present invention, the bag may be a cylindrical shape or a brick shape, and the cylindrical shape is shown in FIG. 1, and in the case of a cylindrical shape, the diameter is 12 to 15 cm and the height is 20 to 30 cm. In the case of a brick form, the width and height may be 10 to 12 cm and the height may be 20 to 30 cm.

The bag may also be made of a composite material of polypropylene (PP), high density polyethylene (HDPE) or polyethylene terephthalate (PET) and polyethylene (PE).

In the present invention, micro pores are formed in the bag for cultivation of mushrooms by laser treatment, and the top is opened.

In the present invention, the laser treatment may be performed by using a CO ₂ laser, a UV laser, or a femtosecond laser so as to form micropores having an air permeability of 20,000 to 2,000,000 cc / m ² per day. The micropores 20 have a diameter And may be from 5 to 300 micrometers ([mu] m). The distance (30) between the centers of the micropores and the centers of the adjacent micropores may be 0.1 to 1 mm. When the distance between the centers is less than 0.1 mm, there is a problem that the inflow of pollutant increases. When the distance exceeds 1 mm, the aeration amount per day required for the growth of mushroom mycelium is less than 20,000 ~ 2,000,000 cc / m ² , It has been confirmed through experiments that the distance between the center of the micropores and the center of the adjacent micropores is preferably 0.1 to 1 mm.

In the bag for cultivation of mushrooms, the bottom surface capable of coming into contact with the bottom is preferable as it has no pores. As a result of cultivating the mushroom seedlings, when micro pores existed on the bottom of the mushroom culture bag, the occurrence of contaminants was high and the efficiency of mycelial growth was low.

Next, the medium is inserted into the bag for cultivation of mushrooms, followed by sterilization and cooling.

Ingredients of the medium include sawdust and other nutrients suitable for mushroom cultivation. A medium suitable for culturing the desired mushroom seed culture may be used.

When the medium to be fed into the bag for cultivation of mushrooms is less than 1,500 cc, there is a problem that the content of the cultured mushroom mycelium is reduced, and the content of the cultured mushroom mycelium is reduced to 2,500 cc. In case of exceeding, the amount of medium to be added is larger than that of the bag, so that it takes a long time for the mycelial mycelium to act on the whole medium during the incubation, and it takes much time for browning and aging.

After the medium is added, the bag into which the medium has been added is sterilized at a temperature of 98 to 120 DEG C for 2 to 6 hours, and then the medium is cooled to a temperature of 18 to 22 DEG C suitable for inoculation of the mushroom strain And cooled. It is cooled to an appropriate temperature depending on the type of mushroom seeds.

Next, after the cooling, three steps of inoculating the mushroom seeds are included.

Inoculate the mushroom seeds with the medium cooled to the appropriate temperature. The type of the mushroom may be, but is not limited to, mushroom mushroom, shiitake mushroom, mushroom mushroom, mushroom mushroom or mushroom mushroom.

Next, after the inoculation, the top of the encapsulation front and the top of the back bottom are combined and then sealed by compression heating.

After inoculating the mushroom seeds, the open top and bottom tops of the open bags are combined and sealed with compression heat to form the seal 10. Even if the bag is sealed, the amount of air per a day is maintained at 20,000 to 2,000,000 cc / m ² by micropores formed in the mushroom culture bag at a diameter of 5 to 300 micrometers (μm), thereby removing the carbon dioxide And circulation of air such as oxygen is enabled. In addition, the fine pores having the diameter of 5 to 300 micrometers (μm) as described above can effectively prevent the inflow of contaminants by circulating the air efficiently.

Accordingly, it is possible to circulate the air in the same manner as the bag for the mushroom with the filter in which the air is circulated by forming the filter on the side of the bag for cultivation of the conventional mushroom, and compared with the filter-attached bag requiring about 700 won for the cost of the filter , The cost can be reduced. In addition, compared with the method of using cotton for circulating air, it is simple and effectively prevents inflow of contaminating bacteria.

Next, after the above-mentioned sealing, the inoculated mushroom seeds are cultured in an arm to produce a mycelium.

After the above-mentioned sealing, the inoculated mushroom seeds are cultured in an arm, and cultivation is carried out until the whole mycelium is activated. The above-mentioned arm culturing comprises a step of culturing the cells at a temperature of 20 to 25 DEG C at a humidity of 50 to 70%, and culturing is carried out at an appropriate temperature and humidity depending on the type of mushroom seeds.

FIG. 3 shows that mycelia are formed by inoculating shiitake mushroom seeds into a bag with micropores according to the present invention, and then culturing under a dark culture condition. Fig. 4 shows that the mycelium has grown to the entire culture medium to complete the primary culture.

As described above, it was confirmed that the mycelium cultured in the mushroom culture bag having fine pores with a diameter of 5 to 300 micrometers (μm) so that the peritoneal per day amount was maintained at 20,000 to 2,000,000 cc / m ² .

Next, the cultured mycelium is cultured and then subjected to browning and maturing.

 Fig. 5 shows that the mycelium cultured in the primary culture in Fig. 4 was browned and matured under light culture conditions.

Thereafter, the mycelial body culturing process is carried out. This is a step of culturing the mushroom mycelium which has undergone cancer culture by providing sunlight or light, followed by browning and maturing.

In order to proceed with the aging process, the mycelium which had been firstly cultured was taken out from the bottle or bag, transferred to a separate bag, taken out from the bottle or bag, and then collected in a new bag, However, since the mycelium cultured in the as-cultured state can be browned in the same manner, the mycelium can be agglomerated and stably accumulated in the same bag. It is possible to save the time, manpower and cost, which is economical.

Therefore, the production of the mycelium and browning may proceed in the same bag, thereby saving time, manpower, and cost.

The step of browning and mashing the mycelium cultured in the above manner is a step of allowing the mycelium to form a protective film on its own. As the browning of the medium is evenly spread, the quality of the produced mushroom is improved and the life of the medium is also increased.

The present invention may further comprise a mushroom culturing step of culturing the mycelium cultivated in the above-described manner to obtain a fruiting body.

At this time, the method of matured into fruiting bodies is not particularly limited, and matured fruiting bodies may be matured according to the kinds of mushrooms by a method commonly used in the technical field to which the present invention belongs. For example, the environmental conditions such as humidity, temperature, CO ₂ , and light required when the strain is converted into a fruiting body are appropriately regulated and cultured for several days.

In the present invention, the top of the bag having the browning-aged mycelium formed therein is opened, and the fruit body is matured in the same bag.

As described above, the micro pores are easily formed in the bag according to the present invention by a laser treatment without a filter or cotton, and the mushroom is cultivated, the cost can be reduced and the workability can be improved, and manpower saving and economical efficiency can be secured .

In addition, the micro-pores formed by the laser treatment can provide sufficient ventilation compared to the use of ordinary pouches having no fine pores when cultivating various mushrooms such as mushroom mushroom, oyster mushroom, and shiitake mushroom. .

In addition, a bag having an air permeability of 20,000 to 2,000,000 cc / m ² per day has an advantage that the diameter of the micropores is 5 to 300 micrometers (μm), and the pollution can be reduced while shortening the culture period.

1 shows a perspective view of a cylindrical mushroom culture bag of the present invention.
Fig. 2 is a perspective view of a mushroom culture bag of the present invention in the form of a brick.
FIG. 3 shows that mycelia are formed by inoculating shiitake mushroom seeds into a bag with micropores according to the present invention, and then culturing under a dark culture condition.
Fig. 4 shows that the mycelium of Fig. 3 grows and is deposited on the entire medium to complete the primary culture.
Fig. 5 shows that the mycelium cultured in the primary culture in Fig. 2 was browned and matured under light culture conditions.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

Example  One

Using a UV laser, a mushroom culture bag with fine pores having a diameter of 5 micrometers (μm) was produced so that a throughput per day was 20,000 cc / m ² .

Example  2

A UV-laser was used to produce a bag for culture of mushrooms having fine pores with a diameter of 10 micrometers (μm) so as to form a throughput of 30,000 cc / m ² per day.

Example  3

A UV-laser was used to prepare a bag for culture of mushrooms having fine pores with a diameter of 30 micrometers (μm) so as to form an aeration amount of 100,000 cc / m ² per day.

Example  4

Using a UV laser, a mushroom culture bag with fine pores having a diameter of 50 micrometers (μm) was produced so that a throughput per day was 160,000 cc / m ² .

Example  5

A UV-laser was used to produce a bag for culture of mushrooms having fine pores with a diameter of 100 micrometers (μm) so as to form a throughput of 400,000 cc / m ² per day.

Example  6

Using a UV laser, a mushroom culture bag with fine pores having a diameter of 150 micrometers (μm) was produced so that a throughput per day was 650,000 cc / m ² .

Example  7

A UV-laser was used to produce a mushroom culture bag having fine pores with a diameter of 200 micrometers (μm) so that a throughput per day was 1,000,000 cc / m ² .

Example  8

A UV-laser was used to produce a mushroom culture bag with fine pores of 250 micrometers (μm) in diameter so that a throughput of 1,450,000 cc / m ^{2 was} formed per day.

Example  9

Using a UV laser, a mushroom culture bag with fine pores having a diameter of 300 micrometers (μm) was produced so that a throughput per day was 2,000,000 cc / m ² .

Comparative Example  One

Using a UV laser, a mushroom culture bag with micropores having a diameter of 1 micrometer (μm) was produced so that a throughput per day was 2,000 cc / m ² .

Comparative Example  2

Using a UV laser, a mushroom culture bag with micropores having a diameter of 4 micrometers (μm) was produced so as to form a throughput of 15,000 cc / m ² per day.

Comparative Example  3

Using a UV laser, a mushroom culture bag having micropores having a diameter of 320 micrometers (μm) was produced so that a throughput per day was 2,500,000 cc / m ² .

Comparative Example  4

A UV-laser was used to produce a mushroom culture bag having fine pores with a diameter of 360 micrometers (μm) so as to produce an air throughput of 3,200,000 cc / m ² per day.

Comparative Example  5

Using a UV laser, a mushroom culture bag with micropores having a diameter of 400 micrometers (μm) was produced so that a throughput per day was 4,200,000 cc / m ² .

The above examples and comparative examples are summarized in the following table.

Micro pore size (micrometer, μm) Daily aeration (cc / m ² ) Example 1 5 20,000 Example 2 10 30,000 Example 3 30 100,000 Example 4 50 160,000 Example 5 100 400,000 Example 6 150 650,000 Example 7 200 1,000,000 Example 8 250 1,450,000 Example 9 300 2,000,000 Comparative Example 1 One 2,000 Comparative Example 2 4 15,000 Comparative Example 3 320 2,500,000 Comparative Example 4 360 3,200,000 Comparative Example 5 400 4,200,000

Experimental Example  1: the incubation period according to the micropore size and Contamination rate

The incubation period and the contamination rate according to the micropore sizes according to Examples 1 to 9 and Comparative Examples 1 to 5 were measured.

First, 2000 cc of shiitake medium was put into the bag, sterilized at 100 ° C for 4 hours, and cooled to 20 ° C. The shiitake mushroom was inoculated on the cooled medium. Thereafter, the top of the encapsulation front and the top of the rear top were combined and then sealed with compression heat. After the sealing, the inoculated mushroom seeds were cultured at a temperature of 23 캜 at a humidity of 60% and culture was carried out until the mycelia were activated throughout the medium. After that, the cultured mycelium was cultured in a dark place without being transferred to a new bag, and the browning period and the contamination rate until this process were measured. The bag was then opened to mature the fruit body.

As a result, in one embodiment of the present invention, a bag having a micropores diameter of 5 to 300 micrometers (μm) so as to have an air permeability of 20,000 to 2,000,000 cc / m ² per day using a laser, , The incubation period was significantly shortened compared with the case of cultivating the mushroom seeds in a bag having micropores having a diameter of less than 5 micrometers (μm). In addition, there was no significant difference in the contamination rate between the mushroom culture bag formed with the micropores having a diameter of 5 to 300 micrometers (μm) and the bag formed with less than 5 micrometers (μm).

Further, when the mushroom seedlings were cultivated in a bag formed with a diameter of fine pores exceeding 300 micrometers (μm) as compared with a bag for cultivation of mushrooms having a diameter of 5 to 300 micrometers (μm) The incubation period was not shortened or increased, and only the contamination rate was significantly increased.

Therefore, the encapsulation of micropores having a diameter of 5 to 300 micrometers (μm) so as to have an air permeability of 20,000 to 2,000,000 cc / m ² per day using the laser shortens the incubation period of the mushroom seedlings and decreases the contamination rate .

The above results are summarized in Table 2.

Incubation period Contamination rate Example 1 90 days 0% Example 2 90 days 0% Example 3 88 days 0% Example 4 85 days 0% Example 5 83 days 0% Example 6 82 days 0% Example 7 81 days 0% Example 8 80 days 0% Example 9 80 days 0% Comparative Example 1 More than 100 days 0% Comparative Example 2 More than 100 days 0% Comparative Example 3 85 days 5% or more Comparative Example 4 85 days 15% or more Comparative Example 5 88 days More than 30%

100: Mushroom culture bag
10: Sealing part
20: Microstructure
30: distance between the center of the micropores and the center of the adjacent micropores

Claims (7)

Preparing a bag for culture of mushrooms having fine pores formed by laser treatment and having open tops;
A second step of putting the medium into the bag, followed by sterilization and cooling;
3) inoculating the mushroom seeds after the cooling;
After the inoculation, the top of the encapsulation front and the top of the back bottom are combined and then sealed by compression heating;
A step 5) of culturing the inoculated mushroom seeds after the above-mentioned sealing step to produce a mycelium; And
Culturing the cultured mycelium and cultivating it by browning.
A method for producing mushroom using a microbial pouch for culture of mushrooms. The method according to claim 1, wherein the bag is made of a composite material of polypropylene (PP), high density polyethylene (HDPE) or polyethylene terephthalate (PET) and polyethylene (PE). The method according to claim 1, wherein the bag has a diameter of 12 to 15 cm and a height of 20 to 30 cm when the bag is cylindrical, and 10 to 12 cm and a height of 20 to 30 cm when the bag is a brick . The method according to claim 1, wherein the laser treatment is performed by using a CO ₂ laser, a UV laser, or a femtosecond laser to form micropores so that the throughput per day is 20,000 to 2,000,000 cc / m ² . The method according to claim 1, wherein the micropores have a diameter of 5 to 300 micrometers (μm). The method according to claim 1, wherein the production of the mycelium and the browning are carried out in the same bag. The method according to claim 1, wherein the encapsulation shortens the incubation period and reduces the contamination rate.

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