KR20190038154A - Genetic Polymorphic marker for predicting moisture contents in skin and use thereof - Google Patents

Abstract

The present invention relates to a specific genetic polymorphic marker having a significant correlation with moisture contents in skin and a composition for detecting the same. According to the present invention, moisture contents in skin can be predicted, and information on skin care and diseases associated with the predicted moisture contents in skin can be provided and utilized in the development of personalized cosmetics.

Description

Genetic polymorphic markers for predicting skin moisture content and uses thereof

The present invention relates to a technique for predicting moisture content in a skin by selecting a specific genetic polymorphic marker having a significant correlation with the moisture content of the skin, A composition for predicting skin moisture content, comprising a marker composition for predicting skin moisture content including a polymorphic marker, an agent for detecting a sequence of the marker, or a kit for predicting skin moisture content, Prediction method.

Genetic analysis research has shifted from research to discovering genetic markers that are relevant to disease or phenotype, to studies that predict future diseases or phenotypes using derived genetic indices. In particular, genetic analysis of facial features and skin characteristics has also increased the number of research fields that predict facial deformity or skin aging (Kemp et al., Molecules, 2017 Feb 26; 22 (3), 2017).

In addition, in research fields that predict phenotypes with a single genetic indicator, more studies are being conducted to increase the prediction accuracy of phenotypes with multiple genetic indices (Siewierska-Gorska et al., Homo. 2017 Feb 4. pii: S0018-442X (17), 2017).

Genetic analysis studies are expanding to basic disease index or academic analysis research, commercial disease prediction services (23andMe, HelloGene), skin prediction services (Pathway Genomics Skinfit, GeneStyle) The combination of platform technology and genomics-based forecasting services in the BT sector is creating new businesses that involve global conglomerates in personalized health and beauty management services.

The present invention provides a composition for predicting the skin moisture content and a kit for predicting the skin moisture content of the composition, which comprises a specific gene polymorphic marker having a significant correlation with the moisture content in the skin, a preparation having a complementary sequence with the sequence thereof .

It is another object of the present invention to provide a method for predicting the moisture content in the skin by identifying the sequence of a specific gene polymorphic marker having a significant correlation with the skin moisture content of a gene sample isolated from a human subject, And to provide a method for classifying types.

The present inventors analyzed the moisture content and genes in the skin of a subject to confirm the nucleotide variation of a gene polymorphism marker located in a gene expected to be related to the skin moisture content and found a gene polymorphism marker having a significant correlation with the skin moisture content And the technique of predicting the skin moisture content based on the information on the pattern of the selected marker among genes of the individual was completed.

The present invention provides a genetic polymorphism marker composition for predicting skin moisture content comprising a marker having a significant correlation with moisture content in the skin.

In one embodiment, the marker comprises a marker in which at least one nucleotide located in the nucleotide sequence of nucleotides 58213463 to 58214463 nucleotides located in at least one of the alleles of human chromosome 17 is mutated; A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 100727203 th nucleotide to 100728203 th nucleotide located in at least one gene of an allele of human chromosome 6 is mutated; A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 135328082-th nucleotide to 135329082-th nucleotide located in at least one gene of an allele of human chromosome 8 is mutated; A marker in which at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 135325607 to 135326607 nucleotides located in at least one gene of an allele of human chromosome 8 is mutated; A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 161738602-th nucleotide to 161739602-th nucleotide located in at least one gene of an allele of human chromosome 6 is mutated; At least one nucleotide located in the nucleic acid sequence consisting of 161739900 nucleotides to 161740900 nucleotides located in at least one gene of the allele of the human chromosome 6 is located in one or more genes of the marker and the allele of the human chromosome 1 And at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 168423343 and 168424343 nucleotides mutated.

The variation of the nucleotide may refer to a homologous or heterozygous wherein the nucleotide comprises a low frequency allele.

More specifically, the marker in which at least one nucleotide located in the nucleic acid sequence consisting of 58213463 nucleotides to 58214463 nucleotides located in at least one of the alleles of chromosome 17 is mutated is one of the alleles of human chromosome 17 At least one marker comprising a single nucleotide polymorphism (SNP) located at the above 58213963 base; A marker in which at least one nucleotide located in a nucleotide sequence consisting of 100727203 nucleotides to 100728203 nucleotides located in one or more genes of the alleles of the human chromosome 6 is mutated is selected from one or more of alleles of human chromosome 6, Lt; RTI ID = 0.0 > SNP < / RTI > A marker in which at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 135328082 to 135329082 nucleotides located in at least one of the alleles of the human chromosome 8 has been mutated is selected from the group consisting of one or more of the alleles of human chromosome 8 Lt; RTI ID = 0.0 > SNP < / RTI > The marker in which at least one nucleotide located in the nucleic acid sequence consisting of nucleotides 135325607 to 135326607 located in at least one of the alleles of the human chromosome 8 is mutated is selected from the group consisting of one or more of the alleles of human chromosome 8 Lt; RTI ID = 0.0 > SNP < / RTI > A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 161738602 nucleotides to 161739602 nucleotides located in at least one of alleles of the human chromosome 6 is mutated is selected from the group consisting of one or more of the alleles of human chromosome 6 Lt; RTI ID = 0.0 > SNP < / RTI > The marker in which at least one nucleotide located in the nucleic acid sequence consisting of 161739900 nucleotides to 161740900 nucleotides located in at least one gene of the allele of the human chromosome 6 is mutated is selected from 161740400 nucleotides At least one marker comprising a SNP located in a base; A marker in which at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 168423343 and 168424343 nucleotides located in at least one gene of the alleles of the human chromosome 1 is mutated is selected from the group consisting of one or more alleles of human chromosome 1 Lt; RTI ID = 0.0 > SNP < / RTI >

More specifically, the marker is a marker (rs10438768) having nucleotide 58213963 nucleotides located in at least one gene of alleles of the human chromosome 17; A marker (rs34260097) having 100727703 th nucleotide G located in at least one gene of alleles of the human chromosome 6; A marker (rs10100237) in which the nucleotide 135328582 is located in at least one of the alleles of the human chromosome 8; A marker (rs1584255) wherein the 135326107th nucleotide located in one or more genes of the allele of human chromosome 8 is A; A marker (rs6939708) in which the nucleotide 161739102 is located in at least one of the alleles of the human chromosome 6; A marker (rs12664801) in which 161740400 nucleotides are G located in one or more genes of alleles of the human chromosome 6; And a marker (rs12563551) in which the nucleotide 168423843 is located in one or more genes of the alleles of the human chromosome 1 (Table 1).

Marker Chromosome number Chromosomal location Darwin's allele Low frequency allele rs10438768 17 58213963 G C rs34260097 6 100727703 T G rs10100237 8 135328582 A G rs1584255 8 135326107 G A rs6939708 6 161739102 G C rs12664801 6 161740400 A G rs12563551 One 168423843 T C

The major allele in the above table means an allele that can be detected in more than 50% of Koreans and a minor allele means an allele that can be detected in less than 50% of Koreans . The presence of a major allele or minor allele in all two alleles is referred to as homozygote and the two alleles have a multivariate allele and a low frequency Major (minor) / minor (minor) cases are called heterozygotes.

The marker has a significant correlation with the moisture content in the skin. When the nucleotide located in at least one of the two alleles is mutated to have a low frequency allele (Major / Minor or Minor / Minor) Or an average skin moisture content, that is, a skin having a high or low moisture content in the skin when both alleles have a major allele (Major / Major).

The moisture content in the skin refers to the moisture content contained in at least one selected from the group consisting of skin epidermis, dermis and subcutaneous fat layer, and may preferably mean the moisture content of the outermost layer or stratum corneum of the epidermis . The moisture content in the skin may be a major factor for diagnosing the skin condition medically or cosmetically. For example, when the moisture content in the skin is lowered to a certain level or lower, it may cause atopic dermatitis, inflammation, dryness, itching, And the like.

The moisture content in the skin may be calculated by measuring the electric conductivity according to the moisture content, and may be a result measured using, for example, a water meter or a skin touch device.

The human normal or average skin moisture content refers to the average value of the skin moisture content measured for 400 subjects having alleles of all two alleles. For example, when electric conductivity is applied to the skin surface, The moisture content of the skin expressed as a percentage (%) may be 35 to 65%, preferably 40 to 60%.

For example, if the subject has at least one marker selected from the group consisting of rs34260097, rs10100237, rs1584255, and rs12563551, the allele has a low skin moisture content relative to the skin moisture content of the normal sample with all major alleles . For example, if the marker has the marker, the allele gene has a water content of 60 to 99.9%, preferably 83 to 97%, based on 100% of the skin moisture content of a normal sample having a major allele. Of the skin moisture content.

In addition, when having at least one marker selected from the group consisting of rs6939708, rs12664801 and rs10438768, it can be predicted that the allele gene has a high skin moisture content in comparison with the skin moisture content of a normal sample having a major allele have. For example, in the case of having the marker, the allele gene has a water content of 100.1 to 140%, preferably 110 to 135%, based on 100% of the skin moisture content of a normal sample having a major allele, Of the skin moisture content.

The term "skin" as used in the present invention may include, but is not limited to, at least one selected from the group consisting of facial skin, neck skin, hand skin, chest skin, leg skin and foot skin. Preferably, the marker of the present invention can be used to predict the moisture content of the facial skin.

When the marker of the present invention is used to predict the moisture content of the facial skin, different markers may be used depending on the skin located in the U-zone or the T-zone. The U zone means a face region extending from the cheek of the face to the cheek of the other side through the jaw, and the T zone means a face region to which the forehead and the nose are connected.

For example, one or more markers selected from the group consisting of rs10438768, rs34260097, rs10100237, rs1584255, rs6939708 and rs12664801 can be used to predict the moisture content of the skin located in the T zone of the face.

In addition, the marker of rs12563551 can be used to predict the moisture content of the skin located in the U-zone of the face.

As another example of the present invention, there is provided a composition for predicting the skin moisture content, which comprises a preparation for detecting a nucleic acid sequence of a marker having a significant correlation with skin moisture content.

The marker or marker composition may be applied equally to compositions for estimating skin moisture content.

More specifically, the agent may be an oligonucleotide, a primer pair or a probe capable of measuring the nucleic acid sequence of the marker.

As used herein, the term "oligonucleotide" refers to a nucleic acid-based molecule capable of forming a dimer with a marker having a complementary sequence to the nucleic acid sequence of the target gene polymorphism marker.

The term "primer" as used herein refers to a nucleic acid sequence having a short free 3 'hydroxyl group and capable of forming a base pair with a complementary template, Refers to 7 to 50 nucleic acid sequences that function as a starting point. Primers are usually synthesized but can also be used in naturally occurring nucleic acids. The sequence of the primer does not necessarily have to be exactly the same as the sequence of the template, but is sufficiently complementary that it can hybridize with the template. Primers can initiate DNA synthesis in the presence of a polymerization reaction (i. E., A reagent for DNA polymerase or reverse transcriptase and four different nucleoside triphosphates) at the appropriate buffer solution and temperature. The primer of the present invention may have a length known in the art as having a sequence complementary to a marker having a significant correlation with the skin moisture content.

As used herein, the term "probe" refers to a nucleic acid fragment such as RNA or DNA corresponding to a specific base or a few hundred bases that can achieve specific binding (hybridization) with a specific DNA or RNA And is labeled with a detectable conventional label (e.g., a fluorescent substance, a luminescent material, a radioactive isotope, etc.), so that the presence or absence of a specific nucleotide can be confirmed. The probe may be prepared in the form of an oligonucleotide probe, a single stranded DNA probe, a double stranded DNA probe, or an RNA probe. In the present invention, the moisture content of the skin can be predicted through hybridization by hybridization using a complementary probe and a marker having a significant correlation with the skin moisture content. The selection and hybridization conditions of the probes can be modified based on what is known in the art.

For example, the probe comprises an oligonucleotide having a sequence complementary to a consecutive 5 to 100 bp nucleotide sequence in a region from the 58213463 base to the 58214463 base of the human chromosome 17; An oligonucleotide having a sequence complementary to a consecutive 5 to 100 bp nucleotide sequence in a region from 100727203 base to 100728203 base of human chromosome 6; An oligonucleotide having a sequence complementary to a consecutive 5 to 100 bp nucleotide sequence in a region from the 135328082-th base to the 135329082-th base of human chromosome 8; An oligonucleotide having a sequence complementary to a consecutive 5 to 100 bp nucleotide sequence in a region from the 135325607 base to the 135326607 base of human chromosome 8; An oligonucleotide having a sequence complementary to a consecutive 5 to 100 bp nucleotide sequence in a region from positions 161738602 to 161739602 of the human chromosome 6; An oligonucleotide having a sequence complementary to a consecutive 5 to 100 bp nucleotide sequence in a region from the 161739900th base to the 161740900th base of the human chromosome 6; And an oligonucleotide having a sequence complementary to a consecutive 5 to 100 bp nucleotide sequence in a region from the 168423343 th base to the 168424343 th base of the human chromosome 1; And the like.

More specifically, the probe is characterized in that at least one 58213963 base (rs10438768) of the alleles of the human chromosome 17 has C and a sequence complementary to a consecutive 5 to 100 bp base sequence comprising the 58213963 base Oligonucleotides; At least one 100727703 base (rs34260097) of the allele of the human chromosome 6 has G, an oligonucleotide having a sequence complementary to a consecutive 5 to 100 bp nucleotide sequence comprising the 100727703 base; At least one 135328582 base (rs10100237) of alleles of the human chromosome 8 has G, an oligonucleotide having a sequence complementary to a consecutive 5 to 100 bp nucleotide sequence comprising the 135328582 base; At least one 135326107 base (rs1584255) of the allele of the human chromosome 8 has A, an oligonucleotide having a sequence complementary to a consecutive 5 to 100 bp nucleotide sequence comprising the 135326107 base; At least one 161739102 base (rs6939708) of the allele of the human chromosome 6 has C, an oligonucleotide having a sequence complementary to a consecutive 5 to 100 bp nucleotide sequence comprising the 161739102 base; At least one 161740400 base (rs12664801) of the alleles of the human chromosome 6 has G, an oligonucleotide having a sequence complementary to a consecutive 5 to 100 bp nucleotide sequence comprising the 161740400 base; At least one 168423843 base (rs12563551) of the allele of the human chromosome 1 has C and may be an oligonucleotide having a sequence complementary to a consecutive 5 to 100 bp base sequence comprising the 168423843 base.

For example, the primer pair includes a primer consisting of two oligonucleotides having a sequence complementary to two consecutive 5-100 bp nucleotide sequences that do not overlap each other in the region from 58213463 base to 58214463 base of human chromosome 17 pair; A pair of primers consisting of two oligonucleotides having a sequence complementary to two consecutive 5 to 100 bp nucleotide sequences that do not overlap each other in the regions from positions 100727203 to 100728203 bases of human chromosome 6; A pair of primers consisting of two oligonucleotides having a sequence complementary to two consecutive 5 to 100 bp nucleotide sequences not overlapping each other in the region from 135328082 base to 135329082 base of human chromosome 8; A primer pair consisting of two oligonucleotides having a sequence complementary to two consecutive 5 to 100 bp nucleotide sequences not overlapping each other in a region from the 135325607 base to the 135326607 base of human chromosome 8; A pair of primers consisting of two oligonucleotides having a sequence complementary to two consecutive 5 to 100 bp nucleotide sequences that do not overlap each other in a region from positions 161738602 to 161739602 of the human chromosome 6; A pair of primers consisting of two oligonucleotides having a sequence complementary to two consecutive 5 to 100 bp nucleotide sequences which do not overlap each other in the region from the 161739900 base to the 161740900 base of human chromosome 6; And a pair of primers consisting of two oligonucleotides having a sequence complementary to two consecutive 5 to 100 bp nucleotide sequences that do not overlap each other in a region from positions 16842334343 to 168424343 bases of human chromosome 1 Or more.

The primers or probes of the present invention can be chemically synthesized using a phosphoramidite solid support method, or other well-known methods. Such nucleic acid sequences may incorporate additional features that do not alter the basic properties. Examples of additional features that may be incorporated include, but are not limited to, methylation, capping, substitution of one or more nucleic acids with homologues, and modification between nucleic acids.

As another example of the present invention, there is provided a composition for predicting skin moisture content, which comprises a preparation for detecting a nucleic acid sequence of a marker having a significant correlation with skin moisture content, or a composition for skin type classification according to skin moisture content, A composition for screening a cosmetic active ingredient related to moisture content or a composition for the diagnosis of a disease or symptom related to skin moisture content such as atopic dermatitis, psoriasis, inflammation, dryness, itching, peeling or wrinkles, A kit for predicting skin moisture content comprising the composition, or a composition for screening a relevant diagnostic drug.

The marker or marker composition and the agent for detecting the nucleic acid sequence of the marker can be similarly applied to the prediction composition, the composition for screening, the composition for screening, the composition for diagnosis, or the kit.

In one example, the kit may further comprise conventional means for detecting the marker and the agent hybridizing to detect the nucleic acid sequence of the marker. For example, when the kit contains a preparation labeled with a detectable labeling substance as a detection agent for detecting the nucleic acid sequence of the marker, the kit may comprise detection means (for example, fluorescence detection means (fluorescence microscope, PCR, etc.) May be further included.

In one example, the marker

(i) one selected from the group consisting of rs10438768, rs34260097, rs10100237, rs1584255, rs6939708, rs12664801 and rs12563551, such as rs12664801;

(ii) a combination of rs12664801 with at least one member selected from the group consisting of rs10438768, rs34260097, rs10100237, rs1584255, and rs12563551; or

(iii) a combination of rs12664801 with at least two members selected from the group consisting of rs10438768, rs34260097, rs10100237, rs1584255, rs6939708, and rs12563551.

The composition or kit may also include an agent that detects a nucleic acid sequence of one or more markers selected from the group consisting of rs10438768, rs34260097, rs10100237, rs1584255, rs6939708, rs12664801, and rs12563551.

For example, the composition or kit may comprise an agent that detects the nucleic acid sequence of the following marker:

(i) one selected from the group consisting of rs10438768, rs34260097, rs10100237, rs1584255, rs6939708, rs12664801 and rs12563551, such as rs12664801;

(ii) a combination of rs12664801 with at least one member selected from the group consisting of rs10438768, rs34260097, rs10100237, rs1584255, and rs12563551; or

(iii) a combination of rs12664801 with at least two members selected from the group consisting of rs10438768, rs34260097, rs10100237, rs1584255, rs6939708, and rs12563551.

Preferably, the composition or kit is a composition comprising an agent that detects the nucleic acid sequence of the rs10438768, rs34260097, rs10100237, rs1584255, rs6939708 and rs12664801 markers, wherein the compositions or kits for predicting the skin moisture content of the T zone of the face have.

Also preferably, the composition or kit is a composition comprising an agent that detects the nucleic acid sequence of the rs12563551 marker and may be a composition or kit for predicting the skin moisture content of the U zone region of the face.

As another example of the present invention, there is provided a method for predicting a skin moisture content, comprising the step of identifying a nucleic acid sequence of a marker having a significant correlation with a skin moisture content with respect to a gene sample obtained from a human subject, A method of providing skin information, a method of providing skin information, a method of providing skin information, a method of providing skin information, a method of providing skin information, A method for screening a drug for diagnosis of a disease or symptom associated with moisture content.

The marker or marker composition may be applied to the prediction method, the classification method, the screening method, and the information providing method.

The skin moisture content prediction technique according to the present invention predicts the skin moisture content inherited genetically, and grasps the understanding of the skin type classified by the skin moisture content or the symptom, and based on the factors related to the skin moisture content, It can be used to develop personalized cosmetics by providing information on diseases.

The present invention will be described in more detail with reference to the following examples. However, the scope of protection of the present invention is not intended to be limited to the following examples.

Example  1. Gene polymorphism for predicting skin moisture content Marker  quest

1.1 Skin moisture content and sample preparation

To measure the skin moisture content of the subjects, the water content of the T zone and U zone of the face was measured in 400 adults between 20 and 30 years old. Specifically, the moisture content in the skin was obtained by measuring the electric conductivity according to the water content using the skin touch device. In addition, a sample of oral cavity gene was collected using a DNA swab.

1.2 Selection of Significant Gene Polymorphic Markers

The skin average moisture content of Example 1.1 was used as a phenotypic variable and about 900,000 genetic mutation sites were examined using a large SNP chip (PMRA SNP chip). Using linear regression, significant genetic variation was found for mean skin moisture content.

The selected markers for the model output were selected from the three major genotypes: major allele, minor allele, major / major homozygous, major / minor heterozygous, and Minor / Minor homozygous We have one individual, and we have confirmed whether the skin moisture content decreases or increases when each genotype is changed from Major / Major to Major / Minor and from Major Minor to Minor / Minor. When the p-value was significantly lower (<0.0001) in the tendency of decreasing or increasing the skin moisture content, it was selected as a significant genetic factor for this analysis. Seven markers and a T zone A total of 22 markers were obtained from 15 skin markers.

The explanatory power (R square) of the marker was calculated by linear regression analysis using the SPSS program. When the prediction value is calculated using the prediction model, the difference between the actual measured value and the predicted value is called a residual. The smaller the residual is, the higher the explanatory power (R square) It can be interpreted as the degree to which. The excavated markers and their explanatory power (R square) values were calculated.

Marker
T zone (T) /
U zone (U) Major / Major Major / minor minor / minor Effect Size Standard Error R2
(Explanatory power) P
(Reliability) Average
(%) frequency Average
(%) frequency Average
(%) frequency rs10438768 T 44.6 133 55.1 199 56.5 76 6.542 1.477 0.044 0.000 rs13400999 T 54.6 326 42.2 79 45.0 4 -10.954 2.386 0.047 0.000 rs34260097 T 55.8 236 47.9 151 39.7 22 -7.963 1.731 0.047 0.000 rs10100237 T 56.3 207 49.4 162 41.3 39 -7.271 1.565 0.048 0.000 rs1584255 T 57.1 144 51.9 189 42.9 76 -6.835 1.441 0.050 0.000 rs6939708 T 46.9 185 54.3 171 61.6 53 7.341 1.489 0.054 0.000 rs12664801 T 46.9 184 54.4 172 61.7 53 7.418 1.490 0.055 0.000 rs142331737 U 51.8 398 40.8 11 - 0 -10.983 2.522 0.042 0.000 rs77031770 U 51.8 400 39.9 10 - 0 -11.872 2.639 0.045 0.000 rs140591763 U 52.0 364 48.8 40 27.3 3 -5.431 1.206 0.045 0.000 rs149919685 U 51.9 390 42.3 16 - 0 -9.564 2.106 0.046 0.000 rs59055935 U 51.1 379 57.3 26 67.5 2 6.682 1.457 0.047 0.000 rs9342420 U 51.8 402 38.9 9 - 0 -12.885 2.771 0.048 0.000 rs12563551 U 52.7 273 49.6 125 44.1 12 -3.514 0.754 0.048 0.000 rs76164268 U 50.4 316 55.4 91 47.0 2 4.391 0.937 0.049 0.000 rs4312176 U 50.7 347 55.5 61 62.7 3 4.979 1.042 0.051 0.000 rs141353816 U 51.7 404 35.5 6 - 0 -16.228 3.379 0.051 0.000 rs6460581 U 51.7 405 35.5 6 - 0 -16.228 3.375 0.051 0.000 rs11258536 U 51.8 398 39.6 11 - 0 -12.158 2.511 0.052 0.000

If the minor / minor frequency of each marker among the above 22 selected markers is 10 or more and the minor / minor frequency of the marker is not 10 or more, the minor / minor frequency of each marker is 1 or more Genetic variants with a major / minor frequency of 20 or more were selected and the seven markers in the table below were obtained.

The selected markers were rs12563551 as a marker for predicting the skin moisture content of the facial region of the face, six markers of rs10438768, rs34260097, rs10100237, rs1584255, rs6939708 and rs12664801, Seven markers were selected as markers for skin moisture content prediction. The position and variation information of the marker are shown in the following table.

Marker Chromosome number Chromosomal location Darwin's allele Low frequency allele rs10438768 17 58213963 G C rs34260097 6 100727703 T G rs10100237 8 135328582 A G rs1584255 8 135326107 G A rs6939708 6 161739102 G C rs12664801 6 161740400 A G rs12563551 One 168423843 T C

Example  2. Marker  Calculate explanatory power by combination

In order to calculate the explanatory power in the case of using a single marker, explanatory power was confirmed in a combination in which markers are added one by one in order of highest explanatory power. The results for the skin moisture content prediction marker of the T zone region are shown in the table below.

Formula
Explanatory power Rank 1 Explanatory power Rank 2 Explanatory power Rank 3 Explanatory power Rank 4 Explanatory Power Rank 5 Explanatory power Rank 6 Dispersion
Analysis p Explanatory power R2 (A1) (A2) (A3) (A4) (A5) (A6) F1 rs12664801 - - - - - 9.48E-07 0.055 F3 rs12664801 rs6939708 rs1584255 - - - 1.64E-11 0.111 F4 rs12664801 rs6939708 rs1584255 rs10100237 - - 5.98E-11 0.112 F5 rs12664801 rs6939708 rs1584255 rs10100237 rs34260097 - 9.12E-14 0.147 F6 rs12664801 rs6939708 rs1584255 rs10100237 rs34260097 rs10438768 7.47E-16 0.174

As can be seen from the above table, the explanatory power of the combination of F5 and F6 was the best in the T zone skin of the face. In the case of F6, it was confirmed that about 17% of the water content of the T zone region can be explained genetically.

Example  3. Marker  Prediction of skin moisture content by combination

The descriptive power of each marker in Examples 1 and 2 is summarized in Table 6 below and based on this, an expression for predicting the skin moisture content according to the markers as shown in Table 7 was completed.

division Marker R2 (explanatory power) A1 rs10438768 0.044 A2 rs34260097 0.047 A3 rs10100237 0.048 A4 rs1584255 0.05 A5 rs6939708 0.054 A6 rs12664801 0.055 B1 rs12563551 0.048

Combination (Fn) Estimated skin moisture content (%) F1 46.944 + (7.418) * (A1) F3 52.513 + (7.796) * (A1) + (-6.879) * (A2) F4 (A2) + (-3.136) * (A3) 52.894+ (7.521) * (A1) + (-4.575) F5 (A2) + (-3.816) * (A3) + (-6.931) * (A4) 55.860 + 7.322 * F6 (A3) + (- 7.167) * (A4) + (5.363) * (A5) + (+2.977) F7 52.784 + (- 3.514) * (B1)

The combination F1 to F6 is a predicted value for the skin area moisture content of the T zone and the skin moisture content prediction value of the U zone skin area in the case of the combination F7.

Claims (13)

And at least one marker selected from the group consisting of the following markers:
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 58213463 nucleotides to 58214463 nucleotides located in at least one gene of the allele of human chromosome 17 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 100727203 th nucleotide to 100728203 th nucleotide located in at least one gene of an allele of human chromosome 6 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 135328082-th nucleotide to 135329082-th nucleotide located in at least one gene of an allele of human chromosome 8 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 135325607 to 135326607 nucleotides located in at least one gene of an allele of human chromosome 8 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 161738602-th nucleotide to 161739602-th nucleotide located in at least one gene of an allele of human chromosome 6 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 161739900 nucleotides to 161740900 nucleotides located in at least one gene of an allele of human chromosome 6 is mutated; And
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 168423343 and 168424343 nucleotides located in at least one gene of a human chromosome 1 allele is mutated. The method according to claim 1,
The marker in which at least one nucleotide located in the nucleotide sequence consisting of 58213463 nucleotides to 58214463 nucleotides located in at least one of the alleles of the human chromosome 17 is mutated is one of the alleles of chromosome 16 of human 16 At least one marker comprising a SNP (single nucleotide polymorphism) marker located at the above 58213963 base,
The marker in which at least one nucleotide located in the nucleic acid sequence consisting of 100727203 nucleotides to 100728203 nucleotides located in one or more genes of the allele of the human chromosome 6 is mutated is selected from one or more of the alleles of human chromosome 6 At least one marker comprising a SNP located in a base,
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 135328082 to 135329082 nucleotides located in at least one of the alleles of the human chromosome 8 has been mutated is selected from the group consisting of one or more of the alleles of human chromosome 8 Lt; RTI ID = 0.0 &gt; SNP, &lt; / RTI &gt;
The marker in which at least one nucleotide located in the nucleic acid sequence consisting of nucleotides 135325607 to 135326607 located in at least one of the alleles of the human chromosome 8 is mutated is selected from the group consisting of one or more of the alleles of human chromosome 8 Lt; RTI ID = 0.0 &gt; SNP, &lt; / RTI &gt;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 161738602 and 161739602 nucleotides located in one or more genes of a human chromosome 6 allele is mutated, At least one marker comprising a SNP located at position 161739102 &lt;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 161739900 nucleotides to 161740900 nucleotides located in at least one gene of the allele of human chromosome 6 is mutated is selected from the group consisting of at least one of alleles of human chromosome 8 and 6 One or more markers comprising a SNP located in the 161740400 base,
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 168423343 and 168424343 nucleotides located in at least one gene of the alleles of the human chromosome 1 is mutated is selected from the group consisting of one or more alleles of human chromosome 1 Wherein the polymorphism marker comprises at least one SNP located at the base of the polymorphism. The skin moisture content prediction gene polymorphism marker composition according to claim 1, wherein the marker is at least one marker selected from the group consisting of:
A marker (rs10438768) having a nucleotide sequence of 58213963 nucleotides located in at least one gene of alleles of the human chromosome 17,
A marker (rs34260097) having 100727703 nucleotides of G located in at least one of the alleles of the human chromosome 6,
A marker (rs10100237) in which the nucleotide 135328582 is located in at least one of the alleles of the human chromosome 8, G,
A marker (rs1584255) in which the nucleotide 135326107 is located in one or more of the alleles of the human chromosome 8 is A,
A marker (rs6939708) having nucleotide 161739102 C located in at least one gene of alleles of the human chromosome 6,
A marker (rs12664801) having 161740400 nucleotides of G located in at least one gene of alleles of the human chromosome 6, and
(Rs12563551) in which at least one of the alleles of the human chromosome 1 is located in one or more genes and the nucleotide 168423843 is C; A marker for detecting a nucleic acid sequence of one or more markers selected from the group consisting of the following markers:
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 58213463 nucleotides to 58214463 nucleotides located in at least one gene of the allele of human chromosome 17 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 100727203 th nucleotide to 100728203 th nucleotide located in at least one gene of an allele of human chromosome 6 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 135328082-th nucleotide to 135329082-th nucleotide located in at least one gene of an allele of human chromosome 8 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 135325607 to 135326607 nucleotides located in at least one gene of an allele of human chromosome 8 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 161738602-th nucleotide to 161739602-th nucleotide located in at least one gene of an allele of human chromosome 6 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 161739900 nucleotides to 161740900 nucleotides located in at least one gene of an allele of human chromosome 6 is mutated; And
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 168423343 and 168424343 nucleotides located in at least one gene of a human chromosome 1 allele is mutated. 5. The composition for predicting skin moisture content according to claim 4, wherein the marker is at least one marker selected from the group consisting of:
A marker (rs10438768) in which at least one of the alleles of the human chromosome 17 is Cs 58213963 nucleotide,
A marker (rs34260097) in which at least one 100727703 nucleotide of the allele of the human chromosome 6 is G,
A marker (rs10100237) in which at least one of the alleles of the human chromosome 8 is G135328582 nucleotide,
A marker (rs1584255) in which at least one of the alleles of human chromosome 8 is 135326107 nucleotides A,
A marker (rs6939708) in which at least 161739102 &lt; 2 &gt; nucleotides of alleles of the human chromosome 6 are C,
A marker (rs12664801) in which at least 161740400 nucleotides of alleles of the human chromosome 6 are G, and
A marker (rs12563551) in which at least one of the alleles of human chromosome 1 is 168423843 nucleotides C; 5. The composition according to claim 4, wherein the agent is an oligonucleotide, a primer pair or a probe capable of measuring the nucleic acid sequence of the marker. 5. The composition for predicting skin moisture content according to claim 4, wherein the skin is at least one selected from the group consisting of facial skin, neck skin, hand skin, chest skin, leg skin and foot skin. 5. The method according to claim 4, wherein the at least one marker selected from the group consisting of is for predicting skin having a low water content relative to the skin moisture content of a normal sample having all major alleles Composition for predicting skin moisture content:
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 100727203 th nucleotide to 100728203 th nucleotide located in at least one gene of an allele of human chromosome 6 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 135328082-th nucleotide to 135329082-th nucleotide located in at least one gene of an allele of human chromosome 8 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 135325607 to 135326607 nucleotides located in at least one gene of an allele of human chromosome 8 is mutated; And
(Rs12563551) in which at least one of the alleles of the human chromosome 1 is located in one or more genes and the nucleotide 168423843 is C; 5. The method according to claim 4, wherein the at least one marker selected from the group consisting of is for predicting skin having a high water content relative to the skin moisture content of a normal sample having all major alleles Composition for predicting skin moisture content:
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 58213463 nucleotides to 58214463 nucleotides located in at least one gene of the allele of human chromosome 17 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 161738602-th nucleotide to 161739602-th nucleotide located in at least one gene of an allele of human chromosome 6 is mutated; And
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 161739900 nucleotides to 161740900 nucleotides located in at least one gene of an allele of human chromosome 6 is mutated. 5. The composition for predicting skin moisture content according to claim 4, wherein the at least one marker selected from the group consisting of the following is for predicting the moisture content of the T zone zone of the face:
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 58213463 nucleotides to 58214463 nucleotides located in at least one gene of the allele of human chromosome 17 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 100727203 th nucleotide to 100728203 th nucleotide located in at least one gene of an allele of human chromosome 6 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 135328082-th nucleotide to 135329082-th nucleotide located in at least one gene of an allele of human chromosome 8 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 135325607 to 135326607 nucleotides located in at least one gene of an allele of human chromosome 8 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 161738602-th nucleotide to 161739602-th nucleotide located in at least one gene of an allele of human chromosome 6 is mutated; And
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 161739900 nucleotides to 161740900 nucleotides located in at least one gene of an allele of human chromosome 6 is mutated. 5. The method according to claim 4, wherein the marker (rs12563551) having 168423843 nucleotides C located in at least one of the alleles of the human chromosome 1 is used for predicting the moisture content of the U zone skin of the face, Composition for predicting the content. Identifying a nucleic acid sequence of one or more markers selected from the group consisting of the following markers for a gene sample obtained from a human subject:
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 58213463 nucleotides to 58214463 nucleotides located in at least one gene of the allele of human chromosome 17 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 100727203 th nucleotide to 100728203 th nucleotide located in at least one gene of an allele of human chromosome 6 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 135328082-th nucleotide to 135329082-th nucleotide located in at least one gene of an allele of human chromosome 8 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 135325607 to 135326607 nucleotides located in at least one gene of an allele of human chromosome 8 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 161738602-th nucleotide to 161739602-th nucleotide located in at least one gene of an allele of human chromosome 6 is mutated;
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of 161739900 nucleotides to 161740900 nucleotides located in at least one gene of an allele of human chromosome 6 is mutated; And
A marker in which at least one nucleotide located in a nucleic acid sequence consisting of nucleotides 168423343 and 168424343 nucleotides located in at least one gene of a human chromosome 1 allele is mutated. 12. A kit for predicting skin moisture content, comprising the composition of any one of claims 4 to 11.