KR20170123989A - Method for predicting skin barrier property - Google Patents

Abstract

In the specification, disclosed is a method for providing information about a skin barrier property, which comprises a step of measuring one or more of rs3846662 single nucleotide polymorphism (SNP) of a HMGCR gene, rs1799983 SNP of a NOS3 gene and rs9939609 SNP of a FTO gene from a specimen, and when the genotype of a SNP position of the gene is E0, E1 and E2, the skin barrier function is excellent in order of E0>E1>E2. The genotype score S_G from each of the genes is calculated and analyzed.

Description

Method for predicting skin barrier property

The present specification describes a method for predicting skin barrier function.

The skin is a primary barrier for protecting individuals from the outside, and performs a skin barrier function including protection against external stimuli such as ultraviolet rays, chemicals, air pollutants, dry environment, and protection against excessive divergence of body water . The function of the skin barrier function is maintained when the stratum corneum is normally formed.

The stratum corneum (horney layer), which is the outermost part of the epidermis, is formed from keratinocytes and consists of keratinocytes with complete differentiation and a surrounding lipid layer. The keratinocyte is a characteristic cell in which the basal cell that continuously proliferates in the lowest epidermis undergoes a stepwise change in morphology and function and is elevated to the surface of the skin. After a certain period of time, the old keratinocyte is removed from the skin, This process of repetitive series of changes is called "epidermal cell differentiation" or "keratinization".

In addition, during keratinization, keratinocytes form natural moisturizing factors (NMF) and intercellular lipids (ceramides, cholesterol, fatty acids) and form stratum corneum, which gives firmness and flexibility to the stratum corneum, barrier function.

The skin can be changed by external factors such as hormones, immune cell functions, internal factors such as activity, ultraviolet rays, free radicals, active oxygen, etc., and it is possible to prevent such changes in skin condition, Various methods have been performed to maintain or improve the changed skin condition.

However, due to the genetic and environmental influences, the individual skin characteristics are very different, so the effects of using the same method can vary widely. Therefore, it is important to understand individual skin characteristics in order to maintain and improve effective skin condition.

Genetic characteristics of individuals can be diagnosed through genes. For example, diagnostic methods using genetic markers are being conducted to identify the risk of developing a specific disease. In such a method, it is necessary to identify the marker having a relation with the target characteristic.

Furthermore, the skin characteristic is represented by a combination of various genetic characteristics, and there is a demand for a method for determining more accurate skin characteristic by revealing the factors involved in the characteristic.

Korean Patent Publication No. 10-2009-0027537 (Mar. 17, 2009)

In one aspect, a challenge to be addressed by the present invention is to provide information about the skin barrier function that an individual has genetically possessed.

In another aspect, an object of the present invention is to accurately predict an individual's skin barrier function or skin moisture storage ability through a specific genotype.

In another aspect, the object of the present invention is to provide an accurate prediction of the inherent skin barrier function of an individual by reflecting the degree of association of a specific genotype and the degree of influence of a specific gene.

In another aspect, the object of the present invention is to provide an accurate prediction of the inherent skin barrier function of an individual in consideration of the genetic characteristic of each genus, taking into consideration the degree of association of a specific genotype and the influence of a specific gene.

In one aspect of the present invention, as a method for providing information on the skin barrier function,

One or more of rs3846662 single nucleotide polymorphism (SNP) of the HMGCR gene, rs1799983 single nucleotide polymorphism (SNP) of the NOS3 gene and rs9939609 single nucleotide polymorphism (SNP) of the FTO gene were measured from the specimen,

When the genotype of the SNP position of the gene is E0, E1 and E2, the skin barrier function is excellent in order of E0 > E1 > E2,

From each of the genes, the genotype score S _G And analyzing it,

The genotype score S _G If the frequency F _E0 of E0 genotype is less than 45%, if the genotype of the subject is E0 is _{S G = 1, E1 0.5 ≤} S G ≤ 0.8, E2 is 0.2 ≤ S _G <0.5, and the frequency of the genotype of E0 F _{And E0} is 45% or more,

Provides a method of providing information on skin barrier function.

<Formula 1>

Genotype score (S _G ) = F _s / F _E0

F _s is the frequency of the genotype of the specimen, F _E0 is the frequency of the E0 genotype, and F _s and F _E0 are obtained from the genotype database of the race to which the specimen belongs.

In one aspect, the present invention can provide information about the skin barrier function that an individual has genetically possessed.

In another aspect, the present invention can accurately predict an individual's skin barrier function or skin moisture storage ability through a specific genotype.

In another aspect, the present invention can accurately predict the inherent skin barrier function of an individual by reflecting the degree of association of a specific genotype and the degree of influence of a specific gene.

In another aspect, the present invention can accurately predict the inherent skin barrier function of an individual in consideration of the genetic characteristic of each race, taking into consideration the degree of association of a specific genotype and the influence of a specific gene.

FIG. 1 is a diagram illustrating a workflow of a single base polymorphism detection method according to the TaqMan probe method.
Figure 2 shows the nucleotide sequence representing the rs3846662 single nucleotide polymorphism of the HMGCR gene.
Figure 3 shows the nucleotide sequence representing the rs1799983 single nucleotide polymorphism of the NOS3 gene.
Figure 4 shows the nucleotide sequence representing the rs9939609 single nucleotide polymorphism (SNP) of the FTO gene.

Hereinafter, embodiments of the present invention will be described in more detail. However, the techniques disclosed in the present application are not limited to the embodiments described herein but may be embodied in other forms. It should be understood, however, that the embodiments disclosed herein are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention.

In the present specification, &quot; single nucleotide polymorphism &quot; (SNP) refers to a base mutation in which a single nucleotide appears differently in DNA, and shows a deviation depending on an individual. Such single nucleotide polymorphism Protein structure, expression level, or expression pattern, and the like.

In the present specification, the rs number specifying the SNP is a reference number of NCBI (National Center for Biotechnology Information).

As used herein, the term &quot; probe &quot; refers to a polynucleotide, a variant thereof, or a polynucleotide having a base sequence complementary to a target site of a gene and a marker substance bound thereto.

As used herein, the term &quot; primer &quot; refers to a polynucleotide having a base sequence complementary to the end of a specific region of a gene used for amplifying a specific region corresponding to a target region of the gene using PCR Means a variant thereof. The primer does not need to be completely complementary to the end of a specific region and can be used if it is complementary enough to hybridize to the terminal to form a double-stranded structure.

As used herein, &quot; hybridization &quot; means that two single-stranded nucleic acids form a duplex structure by pairing complementary base sequences. Hybridization can occur not only in perfect complementarity between single-stranded nucleic acid sequences, but also in the presence of some mismatching nucleotides.

As used herein, the term &quot; frequency of genotypes &quot; refers to the frequency of occurrence of the genotype in the genetic pool as a percentage. The gene pool may be a pool of whole human species or a gene pool of a particular race or group with a common genetic association. For example, the frequency of genotypes in Asian gene pools can be found at http://asia.ensembl.org.

In one embodiment, the present invention provides a method of providing information on skin barrier function.

In this embodiment, the method of providing information comprises measuring one or more of rs3846662 single nucleotide polymorphism (SNP) of HMGCR gene, rs1799983 single nucleotide polymorphism (SNP) of NOS3 gene and rs9939609 single nucleotide polymorphism (SNP) of FTO gene from a sample And analyzing it.

The information providing method comprises the steps of measuring one or more of rs3846662 single nucleotide polymorphism (SNP) of the HMGCR gene, rs1799983 single nucleotide polymorphism (SNP) of the NOS3 gene and rs9939609 single nucleotide polymorphism (SNP) of the FTO gene from the sample, Genotype score S _G And analyzing it.

The genetic skin barrier function of the skin can be determined according to the base at the SNP position of the gene. By confirming the SNP sequence of the gene, the individual can genetically confirm the inherent skin moisture storage ability, skin moisture evaporation prevention ability, and skin moisture loss prevention ability. By confirming the SNP sequence of the gene, an individual can genetically determine the inherent skin barrier function, thereby confirming the moisturizing and moisture-related characteristics of the skin.

When the genotype of the SNP position of the gene is E0, E1 and E2, the skin barrier function is excellent in order of E0 > E1 > E2, and scores can be given according to the genotype.

The genotype score S _G S _G = 1 if the genotype of the specimen is E0, 0.5 ≤ S _G ≤ 0.8 if E1, and 0.2 ≤ S _G <0.5 if the frequency F E0 of the genotype of _E0 is less than 45%. For example, S _G = 1 for E 0, 0.6 ≤ S _G ≤ 0.7 for E 1, 0.3 ≤ S _G ≤ 0.4 for E 2, S _G = 1 for E 0 and S _G = 2/3 for E 1 S _G = 1/3. However, the genotype score of E0, E1, and E2 is E0>E1> E2. In one embodiment, the genotypic score is a reflection of the frequency of the E0 genotype, the rs3846662 single nucleotide polymorphism (SNP) of the HMGCR gene, the rs1799983 single nucleotide polymorphism (SNP) of the NOS3 gene and the genotype score of the rs9939609 single nucleotide polymorphism Can be appropriately adjusted according to the ratio.

The genotype score S _G can be represented by the following formula 1 when the frequency F of the _E0 E0 genotype is not less than 45%.

<Formula 1>

Genotype score (S _G ) = F _s / F _E0

F _s is the frequency of the genotype of the specimen, and F _E0 is the frequency of the E0 genotype.

Since the genotype score is calculated according to the frequency of the specific genotype as described above and the frequency in the actual gene pool is reflected, more matching information can be provided for the specimen. In particular, it is possible to provide more accurate information by limiting the gene pool to a specific racial or genetic association group to which the specimen belongs.

The genotype score S _G (r) of the rs3846662 single nucleotide polymorphism (SNP) of the HMGCR gene, rs1799983 single nucleotide polymorphism (SNP) of the NOS3 gene and rs9939609 single nucleotide polymorphism Can be reflected at a ratio of 0.5-1.5: 0.5-1.5: 0.5-1.5, for example, at a ratio of 0.8-1.2: 0.8-1.2: 0.8-1.2, for example, 1: 1: 1 .

It is possible to provide more accurate information by reflecting the genotypic score reflecting the frequency of these genotypes at a specific rate instead of simply judging and combining the single nucleotide polymorphisms of the three genes.

In one embodiment, skin barrier function scores can be calculated reflecting genotype scores for the three genes. The rate of reflection of the genotypic score may be as described above. According to the score, the classification regarding the skin barrier function can be classified to provide information on the skin barrier function. In one example, the grades can be divided into good, interest, and attention in order of excellent skin barrier function, but are not limited thereto.

The measurement of the single base polymorphism of the gene from the sample may include identifying a base at the SNP position of each gene from the DNA of the sample. The method for identifying a base is not particularly limited as long as it is a method used for detecting a single nucleotide polymorphism (SNP). For example, the nucleotide sequence may be analyzed by sequencing, , Or a method of hybridizing with sample DNA using a probe complementary to the above sequence to measure the degree of hybridization. For example, TaqMan ^TM using allele-specific hybridization  Probe method using allele-specific hybridization through polymerisation, restriction fragment length polymorphism (RFLP) method using restriction enzymes, melting temperature (Tm) A dynamic allele-specific hybridization (DASH) method, a pyrosequencing method using a polymerization reaction, a microarray method using an allele-specific extension, or the like can be used But is not limited thereto.

In one embodiment, measuring a single base polymorphism of a gene from the sample comprises: (a) obtaining DNA from the sample; (b) amplifying the rs3846662 single nucleotide polymorphism (SNP) of the HMGCR gene, the rs1799983 single nucleotide polymorphism (SNP) of the NOS3 gene and the rs9939609 single nucleotide polymorphism (SNP) region of the FTO gene in the DNA; And (c) identifying a base at the SNP position in the amplified DNA.

The human HMGCR gene has a function of regulating the level of cholesterol in the blood, and rs3846662 SNP of HMGCR exists in the intron region of the gene, and thus there may be a difference in the cholesterol level of the skin according to the SNP. The presence of skin cholesterol acts as a barrier to prevent moisture evaporation from the inside to the outside of the skin, thus affecting skin barrier function. The nucleotide sequence of the HMGCR gene is shown in SEQ ID NO: 1.

The polynucleotide corresponding to rs3846662, which is the single nucleotide polymorphism (SNP) of the HMGCR gene, has a single base polymorphism in which the base is T or C at the SNP position. The genotype of the single nucleotide polymorphic position may be TT, TC or CC.

In the HMGCR gene, if the genotype of the SNP locus is TT, TC or CC, the genetic skin barrier function is excellent in order of TT> TC> CC.

The human NOS3 gene is known as a gene related to the synthesis of nitrogen monoxide, and the rs1799983 SNP of the HMGCR exists in the promoter region of the gene, and thus there may be a difference in the function of synthesizing nitrogen monoxide (NO) according to the SNP. Nitric oxide synthesis regulates vascular function and blood circulation, and thus water loss due to heat dissipation through the skin can be controlled. The nucleotide sequence of the NOS3 gene is shown in SEQ ID NO: 2.

The polynucleotide corresponding to rs1799983, which is the single nucleotide polymorphism (SNP) of the NOS3 gene, has a single base polymorphism in which the base is G or T at the SNP position. The genotype of the single nucleotide polymorphic position may be GG, GT or TT.

In the NOS3 gene, when the genotype of the base of the rs1799983 single nucleotide polymorphism position is GG, GT or TT, the genetic skin barrier function is excellent in order of GG> GT> TT.

The human FTO gene acts in conjunction with IRX3 and is known to play a role in conventional obesity. The rs9939609 SNP of the FTO is present in the intron region of the FTO gene. The present inventors have found that the rso9939609 SNP of the FTO gene is involved in the moisture storage of the skin. The nucleotide sequence of the FTO gene is shown in SEQ ID NO: 3.

The polynucleotide corresponding to rs9939609, which is the single nucleotide polymorphism (SNP) of the FTO gene, has a single base polymorphism in which the base is A or T at the SNP position. The genotype of the single nucleotide polymorphic position may be AA, AT or TT.

In the FTO gene, when the genotype of the SNP position is AA, AT, or TT, the genetic skin barrier function is excellent in order of AA> AT> TT.

In one embodiment, one or more of a primer and a probe may be used to detect a base at the SNP position of the gene.

The probe may comprise 20 to 60, for example 40 to 52, for example 45 to 50 consecutive nucleotide sequences comprising a base at the SNP position of the nucleotide sequence, or a sequence complementary to the nucleotide sequence . The probe may have a sequence that is capable of hybridizing to the SNP sequence to detect a base at the SNP position. The probe may comprise a polynucleotide having such a sequence and a labeling substance bound thereto for detection.

The primer may comprise a set of primers consisting of two polynucleotides having a sequence complementary to each of 10 to 40 consecutive nucleotide sequences at the 5 'and 3' ends of the gene fragment of the target site. The primer set is used for amplification of a target site, and the target site is a contiguous base sequence comprising a base at the SNP position of the gene, for example, 50 to 400 consecutive bases containing a base at the SNP position Base sequence. The target site may be, for example, 100 to 300 consecutive nucleotide sequences, including, for example, 200 consecutive nucleotide sequences containing a nucleotide at the SNP position.

According to the embodiments of the present invention, it is possible to provide information on skin barrier function and skin moisture retention ability genetically possessed by an individual. Specifically, it is possible to identify a single nucleotide polymorphism of a specific gene related to the skin barrier function, in particular, the skin moisture storage ability, thereby providing information on an individual's natural skin characteristics, thereby providing management, And the like.

Hereinafter, the present invention will be described in detail with reference to Examples, Comparative Examples and Test Examples. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not to be construed as limiting the scope of the present invention. It will be self-evident.

[Test Example 1] DNA extraction and amplification from a specimen

Genomic DNA was extracted from blood of subjects using blood DNA (QIAamp DNA Blood Mini Kit, Quiagen) for 108 Koreans. The extracted DNA was purified and used in the following experiments.

[Test Example 2] Detection of gene SNP

Using the DNA of the subject amplified and purified in Test Example 1 as a template, the SNP of each gene was detected by the TaqMan probe method as shown in Fig.

Specifically, to 20ng of the genomic DNA of the subject obtained in Test Example 1, 12.5 占 of 2X TaqMan Universal PCR Master mix, 1.25 占 퐇 of 20X Primer and TaqMan Probe dye mix of TaqMan SNP Genotyping Assay specific to each gene SNP shown in Table 1 below , And 11.25 μl of DNase-free sterile water. The final reaction solution was adjusted to 25 μl by vortexing. The reaction solution was dispensed into a 96-well plate to perform PCR reaction. The PCR conditions were 95 ℃ for 10 min, followed by denaturation at 95 ℃ for 15 sec and annealing and extension at 60 ℃ for 40 cycles. After that, gene SNP was detected using a real-time PCR system (TaqMan Mastermix, ABI).

Gene name Reference SNP TaqMan SNP Genotyping Assay ID HMGCR rs3846662 C___2838669_10 NOS3 rs1799983 C___3219460_20 FTO rs9939609 C__30090620_10

[Test Example 3] Genotype score calculation and analysis

According to the SNP genotype identified in Test Example 2, the genotype score S _G Respectively.

Gene name Reference SNP SNP location genotype E0 E1 E2 HMGCR rs3846662 TT TC CC NOS3 rs1799983 GG GT TT FTO rs9939609 AA AT TT

The frequencies (F _E0 , F _E1 , and F _E2 ) for each of the SNP genotypes in Table 3 are obtained from http://asia.ensembl.org and if the frequency F _E0 of the genotype of _E0 is less than 45% If E0 is greater than S _G = 1, E1 is S _G = 2/3, E2 is S _G = 1/3, the frequency F _E0 than 45% of the genotype of E0, was calculated by the following formula (1).

<Formula 1>

Genotype score (S _G ) = F _s / F _E0

The genotype score of each gene was reflected at a ratio of 1: 1: 1, and scores on skin barrier function were shown in Table 3 below.

gene Reference SNP E0 E0 Score E1 E1 Score E2 E2 Score HMGCR rs3846662 TT 33.0 TC 17.2 CC 15.8 NOS3 rs1799983 GG 33.0 GT 9.8 TT 0.8 FTO / IRX3 rs9939609 AA 33.0 AT 22.0 TT 11.0

 [Test Example 4] Evaluation of skin barrier function

The skin barrier function of the subject was evaluated by the following method.

[Test Example 5] Correlation between Skin Barrier Function Score and Skin Moisture Storage Capacity

The correlation between the skin barrier function score calculated in Test Example 3 and the skin barrier function measured in Test Example 4 was analyzed.

From the above results, it can be seen that information on the skin barrier function can be more accurately provided to the individual by discriminating the skin barrier function through the genotype score in consideration of the genotype frequency of the specific gene SNP of the present invention.

<110> AMOREPACIFIC CORPORATION <120> Method for predicting skin barrier property <130> 16P192 / IND <160> 3 <170> KoPatentin 3.0 <210> 1 <211> 1001 <212> DNA <213> Homo sapiens <400> 1 ttttttttct tactaattta cctctgtgga gctattcatt tgaatcaaca ttcttttttt 60 ccccccaacc aagcataaat attactcatt ttaaatgaat ggctttaaag ttgatattct 120 gttatgtgct ctttagcagg taatatgtta acaattatgt ttggtaatca cagaaaatga 180 cactggttct aaaataaaca aatagatata actgtacata caaatccact cacacacctg 240 ctagtgctgt caaatgcctc ctttatcact gcgaaccctt cagatgtttc gagccaggct 300 ttcacttctg cagagtcaca agcacgtgga agacgcacaa ctgggccacg agtcatccca 360 tctgcaagga ctcggctgct ggcacctcca ccaagctaca cagtatatgt tagagaagca 420 agcacatgtt acccaaaaat gctcatgctt gacccaaaag gtatcactaa ttgtccttaa 480 aactcttctc attgccttac ytatgatgta tttttaaact ggcaaatata taaatgccaa 540 cttacaccta ttgctctgca gcctctattg gtgctggcca caagacaacc ttctgttgtt 600 gccattggaa cctgaaattc tttttcatct aagcaaaggg gtcctgccac tccaacaggg 660 atgggcatat atccaataac attctcacaa caagctccca tcacctaaaa ggtaaagtca 720 ggcaccaaat gaaaatctat atagtaaatg cacaaaattt tatctcagct tgtcagtata 780 actatcttca aacttaatcc tttagtatgt attcttttta aacaaaatgt ttaattcacc 840 tttaaaaagt gtttaaaata ctaaaatctt tgagtaatga ctataaaagc aggaaatata 900 tttttaattt ctatgaccta catcataagc agaataaaaa attaggataa aatgatttaa 960 aaggaaaatg ttttataaaa ctatgtacat atatgaaact a 1001 <210> 2 <211> 1001 <212> DNA <213> Homo sapiens <400> 2 tccccatgcg tgccagctcg gccatcacag tgttcccgca gcgctgccct ggccgaggag 60 acttccgaat ctggaacagc cagctggtgc gctacgcggg ctaccggcag caggatggct 120 ctgtgcgggg ggacccagcc aacgtggaga tcaccgaggt gggcaccgag ggccacccat 180 gagggtgtcc ccaaggtgga gaatgaggaa accagtggga gaaggctcgg gggatccagg 240 caggaagagg ggagcctcgg tgagataaag gatgaaaaac accaaaggag gggtgcctgg 300 gtggtcacgg agacccagcc aatgagggac cctggagatg aaggcaggag acagtggatg 360 gaggggtccc tgaggagggc atgaggctca gccccagaac cccctctggc ccactcccca 420 cagctctgca ttcagcacgg ctggacccca ggaaacggtc gcttcgacgt gctgcccctg 480 ctgctgcagg ccccagatga kcccccagaa ctcttccttc tgccccccga gctggtcctt 540 gaggtgcccc tggagcaccc cacgtgagca ccaaagggat tgactgggtg ggatggaggg 600 ggccatccct gagcctctca agaagggcct gcaagggggt gctgatccca caccccaaca 660 cccccaggct ggagtggttt gcagccctgg gcctgcgctg gtacgccctc ccggcagtgt 720 ccaacatgct gctggaaatt gggggcctgg agttccccgc agcccccttc agtggctggt 780 acatgagcac tgagatcggc acgaggaacc tgtgtgaccc tcaccgctac aacatcctgg 840 aggtgaggtg cgggatgggg ctcgggcacc gaatgcacct gtccaaggca ggagtctggc 900 tctcactcca tccccaaaat gccagccacg gggacaatca gagcaggtcc agggttgcct 960 cctaaatggg aactgaggac aagctctaga accactgaag c 1001 <210> 3 <211> 1001 <212> DNA <213> Homo sapiens <400> 3 tgtgttaggc gagatatggt gagtggtttc agaggcttgt gtgagaaagt tgatacactg 60 cccctacccc atgcaaacac acactttctt ttcttatgat gacctttctt taaaagcatt 120 tgttggaata tgagattata ggccataagt gagaaaacaa ctctttttga gctgtgagga 180 atactaggag aggagaaagt gagctgtgtg tgctgcccat ggtggtacgc tgctatggtt 240 ctacagttcc agtcattttt gacagcatgg attcaatgca aaatggcaac acacactctg 300 tatcttttgg cagatcagaa cataatgaaa ataaaataaa aaaattcaaa actggctctt 360 gaatgaaata ggattcagaa gagatgatct caaatctact ttatgagata atgtcctttt 420 taaaaataaa cactaacatc agttatgcat ttagaatgtc tgaattatta ttctaggttc 480 cttgcgactg ctgtgaattt wgtgatgcac ttggatagtc tctgttactc taaagtttta 540 ataggtaaca gtcagaaatg gagtgggaga gcataaaagc aaactgaaat gcaaatagct 600 ggtaccctga agccattaac tttaagctgg ttattcctga cctactgttt ggacataaga 660 tggtagagag gctgagtgtg acttgaacat ttgttcctta gaaacaccat ccttgggctg 720 ggcgcagtgg ctcacgcctg tgtttccagg actttgggag gctgaggcgg gcagatcatg 780 aggtcaatag atcgaaacta tcctggctaa cacggtgaaa tcccatctct actaaaaata 840 caaaaaatta gccaggtgtg gtggcgggtg cctgtagtcc cagctactcg ggaggctgag 900 gcaggagaat tgcttgaacc caggaggcag aggttgcagt gagccaaggt cgcaccactg 960 cactctagcc tgggcaacag agtgagactc cttctcaaaa a 1001

Claims (8)

A method of providing information on a skin barrier function,
One or more of rs3846662 single nucleotide polymorphism (SNP) of the HMGCR gene, rs1799983 single nucleotide polymorphism (SNP) of the NOS3 gene and rs9939609 single nucleotide polymorphism (SNP) of the FTO gene were measured from the specimen,
When the genotype of the SNP position of the gene is E0, E1 and E2, the skin barrier function is excellent in order of E0 > E1 > E2,
From each of the genes, the genotype score S _G And analyzing it,
The genotype score S _G If the frequency F _E0 of E0 genotype is less than 45%, if the genotype of the subject is E0 is _{S G = 1, E1 0.5 ≤} S G ≤ 0.8, E2 is 0.2 ≤ S _G <0.5, and the frequency of the genotype of E0 F _{And E0} is 45% or more,
How to Provide Information on Skin Barrier Function:
<Formula 1>
Genotype score (S _G ) = F _s / F _E0
F _s is the frequency of the genotype of the specimen, F _E0 is the frequency of the E0 genotype, and F _s and F _E0 are obtained from the genotype database of the race to which the specimen belongs. The method according to claim 1,
Wherein the genotype of the rs3846662 SNP position of the HMGCR gene is TT, TC or CC and the skin barrier function is excellent in order of TT>TC> CC. The method according to claim 1,
Wherein the genotype of the rs1799983 SNP position of the NOS3 gene is GG, GT or TT, and GG > GT > TT. The method according to claim 1,
Wherein the genotype of the rs9939609 SNP position of the FTO gene is AA, AT, or TT and AA > AT > TT. The method according to claim 1,
Measuring the single base polymorphism of the gene from the sample
(a) obtaining DNA from a specimen;
(b) amplifying the rs3846662 single nucleotide polymorphism (SNP) of the HMGCR gene, the rs1799983 single nucleotide polymorphism (SNP) of the NOS3 gene and the rs9939609 single nucleotide polymorphism (SNP) region of the FTO gene in the DNA; And
(c) identifying a base at the SNP position in the amplified DNA. The method according to claim 1,
The genotype score S _G S _G = 1 if the genotype of the specimen is E0, 0.6? S _G ? 0.7 if E1, 0.3? S _G ? 0.4 if the frequency F E0 of the genotype of _E0 is less than 45%. The method according to claim 1,
The genotype score S _G (r) of the rs3846662 single nucleotide polymorphism (SNP) of the HMGCR gene, rs1799983 single nucleotide polymorphism (SNP) of the NOS3 gene and rs9939609 single nucleotide polymorphism Lt; / RTI &gt;
The genotype score S _G for rs3846662 single nucleotide polymorphism (SNP) of the HMGCR gene, rs1799983 single nucleotide polymorphism (SNP) of the NOS3 gene and rs9939609 single nucleotide polymorphism (SNP) of the FTO gene was 0.5-1.5: 0.5-1.5: 0.5- Lt; RTI ID = 0.0 &gt; 1.5. &Lt; / RTI &gt; 8. The method of claim 7,
The genotype score S _G (r) of the rs3846662 single nucleotide polymorphism (SNP) of the HMGCR gene, rs1799983 single nucleotide polymorphism (SNP) of the NOS3 gene and rs9939609 single nucleotide polymorphism 0.8 to 1.2: 0.8 to 1.2: 0.8 to 1.2,
The genotype score S _G S _G = 1 if the genotype of the specimen is E0, 0.6? S _G ? 0.7 if E1, 0.3? S _G ? 0.4 if the frequency F E0 of the genotype of _E0 is less than 45%.

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