KR20190011539A - maufacturing methods of Mugwort essence, and functional food using by the same - Google Patents

Abstract

The present invention relates to a method for manufacturing a wormwood, a wormwood extract, and a functional food using the same. More specifically, the present invention relates to: a method for manufacturing a wormwood extract, which enables elution of useful components of the wormwood, such as eupatilin, jaceosidin, and the like in large quantities by adding jujube and licorice to the wormwood and boiling the same in a water double-boiler; a wormwood extract; and a functional food using the same.

Description

[0001] The present invention relates to a method for preparing a mugwort juice and a functional food using the same,

More particularly, the present invention relates to a method for producing a sponge using medicinal wormwood, and more particularly, to a medicinal herb such as eupatilin and jaceosidin by adding jujube, licorice, Which can be eluted in a large amount, and a functional food using the same.

Mugwort is a natural plant, because it has excellent efficacy. It is also called "herb". It has a rich salt containing various enzymes and nucleic acids. It contains vegetable fiber and protein, high quality minerals and various vitamins. In addition, it is known to improve the constitution of modern people who are acidified with strong alkaline foods, to clear blood, to prevent blood circulation and digestion by preventing the incongruity of the body as a medicine, and to suppress and remove toxic substances harmful to human body. In addition, it contains a lot of vitamins A and C to boost immunity and helps prevent colds.

  The active ingredients of mugwort include isocoumarin, coumarin, diterpenlactone, flavonoid, phellandrene, couprol, cadinene, cineole, cineol, artemisinin, eupatilin, and jaceosidin have been identified.

  The pharmacological action of mugwort showed that the extract of mugwort showed the anticoagulant effect and the flavone extracted from mugwort had the anticancer effect. It also has antimicrobial and antioxidant properties and is effective against allergies and inflammation.

  In addition, Eupatilin is involved in the increase, decrease and pharmacological activity of blood glucose, and it has been reported that the gastric wall-protecting action by promoting gastric mucus secretion is 3.5 times stronger than the conventional cimetidine.

  In addition, jaceosidin is an effective ingredient for the treatment of diseases related to angiogenesis such as rheumatoid arthritis, chronic inflammation, diabetic retinopathy, and hemangioma.

 Conventionally, foods using mugwort have a problem in that they can not sufficiently exhibit the usefulness of ingredients contained in mugwort by simply using mugwort after drying and then pulverizing or using its hot water extract.

In addition, various conventional wormwood foods have problems in that their turbidity is poor, they are large in debris, their bitter taste is too strong, and their flavor is too thick. Especially, common mugwort has a limitation that it can not lead to excellent effects in the treatment of cancer and gastric ulcer.

Korean Patent No. 10-0844373 Korean Patent No. 10-1133549

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in view of the above problems, and it is an object of the present invention to extract a large amount of useful components such as eupatilin and jaceosidin.

Further, the present invention is to provide a mugwort juice having an excellent effect on physiological activity because it contains a large amount of an oil component, and also has an excellent turbidity and less bitter taste due to less residue, thereby having an excellent taste and flavor.

According to one embodiment of the present invention, there is provided a method for producing a scalp wax solution comprising aging wormwood for 1 to 5 years; And adding the jujube and licorice to the aged mugwort, heating and warming the mixture.

The step of aging the mugwort comprises: washing the mugwort mugwort, and drying the water mugwort in the shade; And aging the dried wormwood for 3 years.

The above boiling water is added to about 70 to 95 parts by weight of the aged mugwort, about 15.0 to 17.0 parts by weight of the aged mugwort, about 0.5 to 3.0 parts by weight of the jujube, about 0.5 to 2.0 parts by weight of licorice, Lt; 0 > C for about 30 to 35 hours.

In another embodiment of the present invention, rice, malt, motherwort, Angelica gigas, and ginger are mixed with the shin juice prepared according to the above-mentioned method of making salty shiitake, wherein about 1 part by weight of the salty shiitake is about 8.3 parts by weight of rice, About 0.4 part by weight, about 0.1 part by weight of motherwort, about 0.1 part by weight of Angelica keiskei, and about 0.1 part by weight of ginger.

In the present invention described above, it is possible to extract a large amount of useful components such as eupatilin and jaceosidin, which are useful ingredients of mugwort, by aging the mugwort mugweed for several years and then adding jujube and licorice thereto to the mugwort There is an effect.

In addition, since the ascorbic acid solution prepared by the method of the present invention contains a large amount of an oil component, it has not only an excellent effect of physiological activity but also a good turbidity due to not much residue, and an excellent bitterness and excellent taste and flavor.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart illustrating a method for preparing fortified marshmallow juice using fortified lepidoptera mugwort produced according to an embodiment of the present invention.
FIG. 2 is a photograph showing the rapeseed mugwort which has undergone a three-year aging process according to one embodiment of the present invention.
Figure 3 is a graph showing the results of a comparison between the intensified lavabo mugwort extract (Example 1) (3a), the fortified lambskin mugwort mellifera (Example 2) (3b) (3c). ≪ / RTI >
Figure 4 is a graph showing the results of a comparison between the intensified lavender moxibustion solution (Example 1) (4a), the intensified ladle mugwort mellifera (Example 2) (4b) ) ≪ / RTI > (4c). ≪ Desc / Clms Page number 7 >
Figure 5 shows an example of an enriched lepidoptera including the fortified lepidoptera mugwort extract 5a prepared according to one embodiment of the present invention and the fortified lepidoptera municipal watershed 5b and the fortified lepidoptera essential wort essential water (Comparative Example 3) 5c. High Performance Liquid Chromatography (HPLC) of the fractions.
6 (a), 6 (b), 6 (b), 6 (b), 6 (b) and 6 (b) show the comparison of the properties of the fortified mash mash mash liquid prepared according to the embodiment of the present invention and the mash of Comparative Example 2 ).

BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and will be described in detail in the detailed description. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

 The terminology used in this application is used only to describe a specific embodiment and is not intended to limit the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In the present application, the terms "comprises" or "having" and the like are used to specify that there is a feature, a number, a step, an operation, an element, a component or a combination thereof described in the specification, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

The terms first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart showing a method for producing a scalp solution prepared according to an embodiment of the present invention. FIG.

As shown in the figure, the method for preparing the saline solution according to the present invention basically includes the aging step (S30) and the heating step (S60).

One embodiment of the present invention may further include a washing step (S 10) and a drying step (S 20) before the aging step (S 30), and after the aging step (S 30) It is also possible to further include step S50.

That is, the method for producing the skimmed milk according to the present invention comprises the steps of washing, drying (S20), aging (S30), cutting and washing (S40), mixing (S50) . ≪ / RTI > More particularly, the present invention relates to a process for the production of a medicament which comprises the step of spraying wipes (S10), the step of drying the wrung worms (S20), the step of aging the dried wrights (S30) (S50) of mixing jujube, licorice, and water in mugwort, and heating the mixed mixture to heat the mixture (S60).

Hereinafter, the method of the present invention will be described in detail in order.

First, the present invention may include washing step (S10). The water washing step (S10) is to wash water. The washing method is not particularly limited and includes various methods known in the art. For example, the wormwood can be washed in running water two or three times in total. Washing wormwood is to increase the ripening effect by filtering foreign matter before aging wormwood.

And, the present invention relates to a method for producing a seaweed using wormwood, and the wormwood is not particularly limited and may include various ones known in the art. Among them, it is preferable to use fortified lepidoptera. As used herein, the term " fortified lepidoptera "refers to ragweed grown in Ganghwa Island. Compared to other mugworts, Mugwort mugwort is characterized by its excellent color and leaf shape. Compared with normal mugwort, the strong lepidoptera mugwort has strong myrrh. It is surrounded by the sea and surrounded by the sea. It is considered to be the best cultivar among the wormwood since it has good weatherability due to its marine climate and fountains that grow in the sea, have. Among them, the most preferred is the fortune-telling mugwort collected in the spring before and after the dong.

The enhanced rapeseed mugwort of the present invention has an efficacy in preventing and treating various diseases, and in an action aspect, it has an effect of suppressing hyperactivity such as analgesia, sedation, papermaking through inhibitory action. Enhanced lysozyme mugwort contains various physiologically active ingredients such as flavonoids such as eupatilin and jaceosidin, and phenylpropanoid such as sesamin. Among them, Eupatilin is involved in the rise, fall and pharmacological activity of blood glucose, and it has been reported that the gastric wall protecting action by promoting gastric mucus secretion is 3.5 times stronger than the conventional cimetidine. Eupatilin is an indicator component of mugwort mugwort, which is contained only in a large amount of mugwort native to the west coast. It is an indicator component of gastritis and gastric ulcer treatment, anti-inflammatory activity, suppression of immune response, hepatocytes induced by bile acid, gastric cancer cells, It has excellent efficacy for activities such as apoptosis. In addition, jaceosidin is an effective ingredient for the treatment of diseases related to angiogenesis such as rheumatoid arthritis, chronic inflammation, diabetic retinopathy, and hemangioma.

Next, the present invention may further comprise a step (S20) of drying the washed wormwood. That is, in the drying step (S20), the washed wormwood is dried. It is intended to increase the preservation period of mugwort, and to increase the taste and flavor of mugwort, by drying mugwort prior to ripening of mugwort. There is no particular limitation on the method of drying mugwort. For example, it can be air-dried in the room or dried using a hot-air dryer. It can be aged for a long time in a warehouse that does not get sunshine in an appropriate amount of sunlight in a plastic house or a garbage, but it is preferable to dry it so that it is not exposed to sunlight as much as possible. Among them, it is preferable to dry in the sun for the first 3 days to remove the moisture contained in the leaves and stems of washed wormwood, and then to dry in the shade at the drying facility. At this time, if the drying facility is provided with rain, shading, moisture prevention, ventilation facilities, drying hood, etc., drying can be performed more efficiently. It is important to dry the mugwort well because the colors of mugwort can vary depending on the varieties of mugwort, the amount of sunshine, the moisture condition, the drying period, the temperature, the degree of grinding, and various operations during processing.

The present invention is characterized by including a step (S30) of aging the wormwood. The mugwort may be water washed and / or dried mugwort, but it is preferable to use water mugwort that has been washed and dried as described above. There is no particular limitation on the method of aging the wormwood. For example, it is possible to store the harvested wormwood in a jar or a container and store and store it under appropriate temperature and humidity conditions for a predetermined period of time. Among them, the ripening period is preferably about one year to about five years, and more preferably about three years.

Fortified lepidoptera includes 19 to 29 essential oils. About 19 essential oils are detected in fresh lepidoptera, while 29 essential oils are found in lepidoptera, which is more than 2 years old. In addition, 1,8-cineole increased about 13-fold by storage for 2 years, and trans-b-cayophyllene, which was the main component of fresh lepidoptera, was not detected. Accordingly, it is preferable that the mugwort is aged for about three years so as to maximally contain useful useful components.

Next, the present invention may further comprise a step (S40) of cutting and washing the aged wormwood. That is, the cutting and washing step (S40) is to cut and clean the aged wormwood. It is possible to further enhance the effect of the heated hot water bath by cutting and washing the warm water bath before the hot water bath is matured. The method and size of the cutting method are not particularly limited. For example, it is possible to wash wormwood, which is about 1 m long, about 1 ~ 10 cm in size, after cutting like leaves and stems. Preferably, the aged mugwort is cut into a size of about 2 to 5 cm as compared with the case where the mugwort is heated and boiled, and then it is more advantageous to extract useful components in mugwort.

In addition, the present invention is characterized by including a step (S50) of mixing jujube with licorice. That is, the mixing step (S50) is to mix jujube, liquorice and water in the aged wormwood or the cut and washed wormwood. By adding jujube and licorice to the wormwood, the bitter taste of the wormwood is alleviated, the taste and flavor are improved, and the texture is improved.

It is important to mix the raw material so that the taste of the mixed material can be improved, the sugar content can be increased, and the bitter and sweet taste can be harmonized while suitably reducing the bitter taste and strong flavor of the wolfberry. In the present invention, the proper mixing ratio of jujube, licorice, and water to the mugwort is about 15.0 to about 17.0 parts by weight of the aged mugwort, about 0.5 to about 3.0 parts by weight of the jujube, about 0.5 to about 2.0 parts by weight of licorice, 50 parts by weight and about 70 parts by weight. Among them, it is more preferable that about 16.0 to about 17.0 parts by weight of aged mugwort, about 1.5 to about 3.0 parts by weight of jujube, about 1.0 to 2.0 parts by weight of licorice and 60 to 65 parts by weight of water are more preferable. It does not.

If the amount of the mugwort is too much, the smell of the mugwort is too strong, the taste is reduced, the texture is decreased due to the taste, and the foreign matter is increased and the turbidity is deteriorated. Also, if the amount of mugwort is too small, the efficacy of mugwort can not be expected. For example, if the mugwort contains more than about 30.0 parts by weight of the mugwort mugwort, if it contains jujube, licorice, or water, the texture of the mugwort is deteriorated due to its strong flavor, taste, and turbidity.

In addition, the present invention is characterized by including a step (S60) of heating and mixing the mixture. That is, in the heating step S60, the mixture of the jujube and licorice is heated and boiled. The mixture of the mixture of jujube, licorice and water is heated by hot water to make the most useful components of the mugwort dissolve to the fullest extent and jujube and licorice are considered.

The method of heating and boiling is not particularly limited and may include various methods known in the art. However, in the present invention, it is preferable that the heating is carried out at a temperature of about 75 ° C to about 95 ° C for about 30 hours to about 35 hours. When the temperature of the water bath is less than 75 캜 and the time is less than 30 hours, there is a disadvantage in that the soluble component of the mugwort is not eluted sufficiently. If the temperature of the water bath exceeds 95 캜 and the time exceeds 35 hours, the starch rises in the mugwort, And is susceptible to deterioration, resulting in poor texture. Therefore, it is preferable to heat the above-mentioned heating at a temperature of about 80 ° C to about 92 ° C for about 32 hours. In this case, the soluble component of the mugwort is also eluted well, and the bitter taste and strong smell of mugwort are appropriately decreased, Can be increased, so that the harmony of the bitter taste and the sweet taste is most effective.

According to the present invention, the mugwort is aged for several years, and then the jujube and licorice are added to the mugwort so that a large amount of useful components such as eupatilin and jaceosidin, which are useful components of mugwort, are extracted There is an effect that can be. That is, the present invention relates to a process for efficiently extracting beneficial ingredients of fortified lysolecithine, comprising the steps of: extracting isocoumarin, coumarin, diterpenlactone, flavonoid, ), Phellandrene, couprol, cadinene, cineol, artemisinin, eupatilin, jaceosidin and the like. There is an effect that a large amount of the component can be eluted.

In addition, since the ascorbic acid solution prepared by the method of the present invention contains a large amount of an oil component, it has not only an excellent effect of physiological activity but also a good turbidity due to not much residue, and an excellent bitterness and excellent taste and flavor.

Another aspect of the present invention is a method for manufacturing a crude oil using a mugwort solution prepared according to the above-described manufacturing method, and a method for preparing the same.

That is, the present invention relates to a method for preparing a rice wine, which comprises mixing rice, malt, motherwort, Angelica gigas, and ginger into a mugwort solution prepared according to the above-described production method, wherein about 8.0 to about 9.0 parts by weight of rice, About 0.1-0.2 parts by weight of Angelica keiskei, about 0.1-0.2 parts by weight of Angelica keiskei, about 0.1-0.2 parts by weight of ginger, and about 0.1-0.2 parts by weight of ginger.

Preferably, about 8.3 parts by weight of rice, about 0.4 part by weight of malt, about 0.1 part by weight of motherwort, about 0.1 part by weight of Angelica keiskei, and about 0.1 part by weight of ginger are mixed with 1 part by weight of dried squid liquid Can be provided.

According to the present invention, it is effective in eliminating the bitter taste of dragonflies, adding the deep taste of dragonflies, amylase enzyme rich in digestion and absorption, and low in calories to control the food quantity. When the mugwort solution prepared by the production method according to the present invention is applied to food such as zucchini, excellent effects can be obtained in the treatment or improvement of anti-cancer, gastric ulcer and gynecological diseases.

Furthermore, the present invention relates to a pulverized product obtained by pulverizing raw mugwort or dried mugwort to a particle size of a specific size; Extracts of dried mugwort in hot water; And the mugwort juice (extract) can be applied to foods to produce functional foods.

In addition, the mugwort juice according to the present invention can be used as a material for imparting functionality to various foods and feeds (or feed additives) such as rice cakes, noodles, drinks, and the like, so that industrial applicability is expected.

For example, when the above-mentioned mugwort juice is used, it is possible to use the above-mentioned mugwort juice, such as rice cakes (Songpyeon, rice cakes, rice cakes, mugwort tortillas, inflorescence, mugwort dumplings), cold noodles, It can be used for a variety of foods such as wormwood confectionery and chocolate. It can also be used as other household items such as mugwort soap, mugwort cosmetics, mugwort drinks, mugwort bean sprouts, and wormwood syrup.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.

< Example  1> Fortified ralph lauren  Mugwort

Strengthened lepidoptera was prepared by washing with water. The washed water liquor was dried in the shade and the dried water liquor wormwood was aged for three years at a low temperature (15-25 ° C) in a conventional manner. The 3-year aged reinforced lepidoptera was cut and washed. The jujube, liquorice, and water were mixed and heated in a hot water bath. At this time, the mixture was mixed with water to make about 17% of mugwort powder, about 3% of jujube, and about 2% of licorice. The mixed material including the above-mentioned reinforced lepidoptera was heated in a hot water bath at a temperature of about 80 째 C to about 92 째 C for about 32 hours.

< Example  2> Fortified ralph lauren  Manufacture

Rice, maltose, motherwort, Angelica gigas, and ginger were mixed with the fortified lavish mugwort solution prepared in Example 1, and about 8.3 parts by weight of rice, about 0.4 part by weight of maltose, about 0.4 part by weight of rice, 0.1 part by weight, Angelica grisea about 0.1 part by weight, and ginger about 0.1 part by weight.

Namely, the fortified leprosy mosquito larvae, motherwort, Angelica gigas, and ginger prepared according to the above Example 1 were put in a pot. The rice was washed and sufficiently poured into water, and then water was added to make a rice cake. Then, the malt was poured into water and rubbed by hand, and it was called for about 2 ~ 3 hours. After pouring the boiled water into the boiled maltose, it was repeated about 2 to 3 times, and then it was sown in a sieve or sacked, and it was squeezed to remove the residue and received maltose water. When the hot pepper that I made was warm, I put it in a hot pot and poured maltous water and stirred it to stir well. After fermentation at a temperature of about 60 ° C for about 7 to 8 hours, only the undiluted solution was separated. The separated juice was soaked in a weak fire so as to make fortified larder.

< Comparative Example  1> When the temperature and time conditions are different Fortified lepidoptera  Manufacture of essence

Comparative Example 1 was prepared in the same mixing ratio of jujube, licorice, and water as in Example 1, and the mixed material was heated at a temperature of about 60 to 75 ° C for about 24 hours to prepare a seaweed.

< Comparative Example  2 > material mixing ratio Fortified lepidoptera  Manufacture of essence

Comparative Example 2 is the same as that in Example 1 except that the above ingredients are mixed together with water such that the mixing ratio of the materials is about 35%, about 3% of jujube, about 2% of licorice, More than the sample.

< Comparative Example  3> by distillation Fortified lepidoptera  extraction

Strengthened lepidoptera was prepared by washing with water. The washed rugged warp mugwort was dried in the shade and the dried warp knotworm mugwort was aged at low temperature (15-25 ° C) for 3 years. Thereafter, the active ingredient was extracted by a distillation method, instead of heating with hot water as in the examples of the present invention, to produce intensely water-soluble essential waxy essential water.

< Experimental Example  1 > Yu Patin ( eupatilin ), Posture osidine ( jaceocidin ) Quantitative analysis

 In order to analyze the content of eupatilin and jaceosidin in rapeseed mugwort, TLC and HPLC were used as follows.

In order to analyze the eupatilin and jaceosidin contents in the fortified lepidopterous mugwort, Example 1 (fortified lion mugwort), Example 2 (fortified lion mugwort mugwort), Comparative Example 3 Essential water) was used. Comparative Example 3 (Enhanced Lepidoptera essential water) is an extract obtained by distilling water-soluble lepidopterous mugwort, which is not water-heated, dried and aged for 3 years in the method of manufacturing drag mugwort according to the present invention, Example 1 (fortified lemon mugwort) and Example 2 (fortified lambskin mugwort) were compared with the extraction amounts of the useful components by the manufacturing method according to the invention.

First, the concentrated mugwort mugwort extract (Example 1), the pressed mugwort mugwort mugwort (Example 2), and the pressed mugwort mugwort essential water (Comparative Example 3) were concentrated under reduced pressure at about 40 ° C or lower. Then, ethyl acetate (EtOAc) and the solvent n - hexane (n -hexane) 1 of solvent divided the first mixed solution and the fraction repeatedly with water by 150 ml 3 times. Among them, ethyl acetate - Samples were prepared by concentration of the hexane (EtOAc- n -hexane) layer pressure-n. 3A to 3C according to an embodiment of the present invention show a process for producing fractions of fortified green lime mugwort (Example 1), fortified lime green mugwort (Example 2), and fortified lime essential water (Comparative Example 3) .

As a result of the fractionation preparation process (FIG. 3), it was found that the enriched lepidopterous mugwort solution (fraction) was obtained through EtOAc / n- hexane fr. (Fraction), which is expected to contain eupatilin and jaceosidin (Example 1), and 99.3 mg of the fortified lycopene (Example 2) and 160 mg of the crude lecithin essential oil (Comparative Example 3) were obtained.

In order to identify eupatilin and jaceosidin in the sample using TLC (thin layer chromatograpy), the following conditions were used.

Use TLC: SiO ₂ TLC

Evaporation solvent: EtOAc- n- hexane = 1: 1

Figure 4 is a graph showing the results of a comparison between the intensified lepromatosophilus extract prepared according to one embodiment of the present invention (Example 1, Figure 4a), the intensified lepidoptera (Example 2, Figure 4b) , Fig. 4c). The results of Thin layer chromatograpy are shown in Fig. As shown in FIG. 4 according to one embodiment, a small amount was confirmed in TLC, and in a small amount in intensified lycopods mugwort (Fig. 4a) and intensified lepidoptera (Fig. 4b). Eupatilin, And the presence of jaceosidin could not be confirmed (FIG. 4C).

< Experimental Example  2> HPLC On by Yu Patin ( eupatilin ), Posture osidine ( jaceocidin ) Quantitative analysis

 In order to analyze the content of eupatilin and jaceosidin in rapeseed mugwort, HPLC was carried out as follows.

In order to analyze the eupatilin and jaceosidin contents in the fortified lepidopterous mugwort, Example 1 (fortified lion mugwort), Example 2 (fortified lion mugwort mugwort), Comparative Example 3 The purified fractions were prepared in the same manner as in the above TLC quantitative analysis experiment by using liquefied natural mugwort essential water.

First, in order to prepare a HPLC analysis sample, the filtrate was filtered through a 0.45 mm membrane filter.

The HPLC analysis conditions for quantitative analysis are as follows.

* Device name: Waters 600S

* Column: Phenomenex C18 column (5 [mu] m, 250 mm x 4.6 mm)

* Detector: UV-detector, 330 nm

Solvent: A - water / B - methanol

* Dissolution rate: 0.6 ml / min

* injection volume: 20 μl

To perform HPLC quantitative analysis, a regression curve of a standard sample was first prepared. Eupatilin and jaceosidin were prepared at concentrations of 10, 20, 50 and 500 ppm (0.01, 0.02, 0.05 and 0.5 mg / ml), respectively. Regression curves were prepared by injecting 20 μl under the same solvent conditions.

Then, the contents of eupatilin and jaceosidin in the fractions were analyzed. The thus prepared sample solution was analyzed under the following solvent conditions under the above HPLC analysis conditions. Each experiment was repeated three times.

 Then, a standard sample of eupatilin was analyzed by HPLC up to concentrations of 10, 20, 50 and 500 ppm (0.01, 0.02, 0.05, 0.5 mg / ml) .

In addition, standard samples of jaceosidin were analyzed by HPLC up to concentrations of 10, 20, 50 and 500 ppm (0.01, 0.02, 0.05, 0.5 mg / ml) and the values obtained in this concentration range were used according to regression curves Respectively.

The results of the HPLC quantitative analysis are shown in FIG. Specifically, FIG. 5A, according to one embodiment of the present invention, shows an HPLC chromatogram of intensified leprosy mosquito larvae (Example 1). As shown in Fig. 5A, the HPLC analysis of the EtOAc / n- hexane fraction of the fortified lambskin mugwort extract (Example 1) showed that the eupatilin was at 22.586 min and the jaceosidin was 18.879 min Was observed.

FIG. 5B shows an HPLC chromatogram of the powdered green alga mugwort (Example 2) according to one embodiment of the present invention. As shown in FIG. 5B, the HPLC analysis of the EtOAc / n- hexane fraction of the fortified lycopershopped mugwort (Example 2) revealed that the eupatilin and jaceosidin were at 22.950 min and 18.304 min, respectively Was observed.

Meanwhile, FIG. 5C according to a comparative example of the present invention shows HPLC chromatogram of the hardened lepidoptera essential water (Comparative Example 3). As shown in FIG. 5C, the HPLC analysis of the EtOAc / n- hexane fraction of the enriched lysolecine essential water (Comparative Example 3) showed no eupatilin and jaceosidin.

Therefore, the results of quantitative determination of eupatilin and jaceosidin in each sample are summarized in Table 1 below.

eupatilin jaceocidin amount (mg / g) * amount (mg / bottle) amount (mg / g) * amount (mg / bottle) Example 1 1.513 + 0.050 0.062 ± 0.002 0.041 0.028 0.042 ± 0.001 Example 2 1.283 + - 0.056 ** 0.127 ± 0.006 0.970 + 0.032 0.096 + 0.003 Comparative Example 3 - - - -

*; Contents of eupatilin and jaceosidin per 1 g of EtOAc-n-hexane fraction (unit: mg)

**: The obtained value is the mean and standard deviation of the experiment results of 3 times

According to the above TLC and HPLC quantitative analysis results, it is possible to increase the extractability of eupatilin and jaceosidin, which are highly physiologically active, in the case of mugwort juice, It can be confirmed that it is a manufacturing method.

< Experimental Example  3> Properties, impurities, Briggs  evaluation

The properties, impurities, and brix of Example 1 (fortified lemon mugwort solution) and Comparative Example 2 prepared according to one embodiment of the present invention were compared.

FIG. 6 is a graph comparing the properties of the fortified lavender extract (Example 1) prepared according to one embodiment of the present invention and the essence of Comparative Example 2 (FIGS. 6A and 6B), the impurities (FIG. 6C) 6D and 6D). 6A and 6B, the left side shows the case of Example 1 (17% of fortified leprosy mugwort) and the right side shows the case of Comparative Example 2 (35% of fortified lepidoptera) It can be confirmed that the color is made thicker. However, in the case of the drink to be consumed, it may be objectionable to be too thick, but it can be confirmed that the color of Example 1 (left, 17% content) is brighter and lighter.

Further, referring to FIG. 6C, it was confirmed that Comparative Example 2 (upper portion) was more turbid than Example 1 (lower portion) because of a large amount of impurities. In the case of Comparative Example 2 (upper part), if the content of reinforcing lavender mugwort is too much, the turbidity is increased due to the increase of the starch, the neck hanging phenomenon is increased when drinking, and the aftertaste may not be calmer. Therefore, it was confirmed that it was appropriate to make the content of reinforced rhizome mugwort 17% as compared with that of Comparative Example 2 as in Example 1.

6d and 6e, Fig. 6d shows the Bricks result of the fortified lion's mugwort solution according to Example 1 including 17% of the fortified lepidoptera mugwort, Fig. 6e shows the content of 35% &Lt; tb &gt; &lt; tb &gt; &lt; TABLE &gt; According to this, Example 1 shows 44 brix (%) and Comparative Example 2 shows 38 brix (%). As a result, it was confirmed that the brick value of Example 1 was higher than that of Comparative Example 2, and that the sugar content was higher. This indicates that Comparative Example 2 has a higher intestinal marshmallow content than that of Example 1, so that it is possible to feel less sweetness of jujube and licorice due to bitter taste.

< Experimental Example  4> Evaluation of taste and flavor of mugwort

Comparative Example 1 (including 17% of fortified lepidoptera, at 60 to 75 ° C for 24 hours), Comparative Example 2 (containing 17% of fortified lepidoptera, 32 hours at 80 to 92 ° C) , Flavor, taste, and texture of fortified lambskin mugwort juice were investigated by using 35% content of mugwort powder, 80 ~ 92 ℃, 32 hours).

(Example 1, Comparative Examples 1 and 2) The sensory evaluation results are shown in Table 2 below.

division color incense flavor Texture Comprehensive likelihood Example 1 8.5 8.2 8.3 8.7 6.4 Comparative Example 1 5.6 5.8 5.3 6.4 5.8 Comparative Example 2 4.6 6.4 4.8 5.2 5.3

As can be seen from the above Table 2, the overall acceptability including the color, flavor, taste, texture and the like of the rapeseed mugwort solution prepared according to Example 1 of the present invention was higher than that of Comparative Example. That is, the highest preference was shown in Example 1 (including the content of 17% of rapeseed mugwort, 80 to 92 ° C, 32 hours).

Although the present invention has been shown and described with respect to certain preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the following claims It will be apparent to those skilled in the art.

Claims (4)

Aging wormwood for 1 to 5 years; And
Adding the jujube and licorice to the aged mugwort, and heating and warming the mixture.
The method according to claim 1,
The step of aging the ragweed,
A step of washing the intestinal mascara and drying the wrung worm in the shade; And aging the dried wormwood for 3 years. &Lt; Desc / Clms Page number 20 &gt;
3. The method of claim 2,
The above-mentioned boiling is carried out by adding 15.0 to 17.0 parts by weight of the aged mugwort, 0.5 to 3.0 parts by weight of jujube and 0.5 to 2.0 parts by weight of licorice in 70 parts by weight of water, And heating for 35 hours.
A process for producing a rice germ extract according to any one of claims 1 to 3, which comprises mixing rice, maltose, motherwort, Angelica gigas, and ginger with 1 part by weight of rice powder, 8.3 parts by weight of rice, 0.4 part by weight of malt 0.1 part by weight of motherwort, 0.1 part by weight of Angelica keiskei, and 0.1 part by weight of ginger.

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