KR20230077327A - Composition and manufacturing method for antioxidant and anti-inflammatory effects, mainly containing complexes of natural substance extracts - Google Patents
Composition and manufacturing method for antioxidant and anti-inflammatory effects, mainly containing complexes of natural substance extracts Download PDFInfo
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- KR20230077327A KR20230077327A KR1020210164487A KR20210164487A KR20230077327A KR 20230077327 A KR20230077327 A KR 20230077327A KR 1020210164487 A KR1020210164487 A KR 1020210164487A KR 20210164487 A KR20210164487 A KR 20210164487A KR 20230077327 A KR20230077327 A KR 20230077327A
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Abstract
Description
본 발명은 천연물질 추출물의 혼합물을 주성분으로 하는 항산화 및 항염증 효능을 갖는 조성물 및 그 제조방법에 관한 것으로, 더욱 자세하게는 종래의 항산화 및 항염제의 문제점을 해소하면서 그 효능을 증진시키기 위해 천연물질을 선별하고 이를 특정한 조건으로 추출 및 조합함에 의해 인체에 유해한 반응을 일으키지 않으면서 우수한 항산화 및 항염 효능을 나타내는 환경 친화적인, 천연물질 추출물의 혼합물을 주성분으로 하는 항산화 및 항염증 효능을 갖는 조성물 및 그 제조방법에 관한 것이다.The present invention relates to a composition having antioxidant and anti-inflammatory effects containing a mixture of natural substance extracts as a main component and a method for preparing the same, and more particularly, to a method for improving the efficacy of natural substances while solving the problems of conventional antioxidants and anti-inflammatory drugs. Composition with antioxidant and anti-inflammatory efficacy as a main component containing a mixture of environmentally friendly, natural substance extracts exhibiting excellent antioxidant and anti-inflammatory effects without causing harmful reactions to the human body by selecting, extracting and combining them under specific conditions, and manufacturing the same It's about how.
염증은 예를 들어, 미생물, 특정 항원, 손상된 세포 또는 물리적 또는 화학적 인자의 침입에 대한 국소화된 조직 반응을 특징으로 하는 것으로, 염증성 반응은 조직 치료를 개시할뿐만 아니라 해로운 작용제 및 손상된 조직 둘 모두를 파괴하고, 희석하거나 또는 격리시키는 역할을 하는 정상적으로 보호적 기전이다. 이러한 염증은 물리적 외상, 감염, 일부 만성 질환 (예를 들어, 건선 및 류마티스 관절염과 같은 자가면역 질환) 또는 외부 자극에 대한 화학적 또는 생리학적 반응 (예를 들어, 알레르기 반응의 일부)으로부터 발생할 수 있다. 염증성 매개체가 혈류 및 국소 혈관의 팽창을 증가시켜, 발적 및 열, 체액의 삼출을 초래하고, 종종 국소 부종, 염증성 부위로의 백혈구 이동 및 통증을 초래할 수 있으며, 많은 질환은 비정상적인 조직 손상 염증을 특징으로 하고 이것에 의해 야기된다. 이러한 상태는 전형적으로 면역 방어 기전의 활성화를 특징으로 하여, 숙주에게 이로운 것보다 더 해로운 영향을 초래할 수 있으며, 일반적으로 다양한 정도의 조직 발적 또는 충혈, 팽윤, 고열, 통증, 가려움증, 세포사, 조직 파괴, 세포 증식 또는 기능 상실과 연관되고 그 예로는 염증성 장 질환, 류마티스 관절염, 다발성 경화증, 건선, 사구체신염 및 이식 거부를 포함한다. 이들 염증성 변화는 혈관활성 아민, 사이토카인, 보체 인자 및 반응성 산소 종과 같은 염증성 매개체와 함께 호중구, 단핵구, 대식세포 및 림프구 같은 세포를 포함하는 일련의 세포 및 생화학적 사건에 의해 촉발되는데, 무엇보다도, 염증은 상처 치유 과정에서 중요한 역할을 하는 것이며 따라서, 염증이 발생한다는 것은 치유되어야 할 손상이 발생하였다는 것이다.Inflammation is characterized by a localized tissue response to, for example, invading microorganisms, specific antigens, damaged cells, or physical or chemical factors, the inflammatory response not only initiating tissue repair but also inducing both detrimental agents and damaged tissue. It is a normally protective mechanism that serves to destroy, dilute or sequester. Such inflammation may result from physical trauma, infection, some chronic diseases (eg, autoimmune diseases such as psoriasis and rheumatoid arthritis), or a chemical or physiological response to external stimuli (eg, as part of an allergic response). . Inflammatory mediators increase blood flow and dilatation of local blood vessels, resulting in redness and heat, exudation of body fluids, and often can result in local oedema, migration of leukocytes to inflamed sites and pain, many diseases characterized by abnormal tissue damaging inflammation and is caused by this. These conditions are typically characterized by activation of immune defense mechanisms, which may result in more deleterious than beneficial effects on the host, and are usually tissue redness or congestion of varying degrees, swelling, hyperthermia, pain, pruritus, cell death, and tissue destruction. , cell proliferation or loss of function, examples of which include inflammatory bowel disease, rheumatoid arthritis, multiple sclerosis, psoriasis, glomerulonephritis, and transplant rejection. These inflammatory changes are triggered by a series of cellular and biochemical events involving cells such as neutrophils, monocytes, macrophages and lymphocytes along with inflammatory mediators such as vasoactive amines, cytokines, complement factors and reactive oxygen species, among other things. , Inflammation plays an important role in the wound healing process, and therefore, when inflammation occurs, an injury to be healed has occurred.
또한, 산화는 호흡하여 몸에 들어온 산소는 몸에 이로운 작용을 하지만 이 과정에서 활성산소가 만들어지며, 지나친 활성산소는 산소를 불안정한 상태에 노출시키는것을 이야기하는데 이는 동물의 몸에 나쁜 영향을 준다. 따라서, 활성산소를 적절히 유지하는 것이 세포의 산화, 세포의 노화를 막는데 주요한 원리로 다루어질 수 있으며, 따라서 항산화 물질에 대한 연구가 지속적으로 이루어 지고 있다.In addition, oxygen that enters the body through oxidation has a beneficial effect on the body, but active oxygen is created in this process, and excessive active oxygen exposes oxygen to an unstable state, which has a bad effect on the animal's body. Therefore, proper maintenance of active oxygen can be treated as a major principle in preventing cell oxidation and cell aging, and therefore, research on antioxidants is continuously being conducted.
상기와 같은 점을 고려하여 종래로부터 항산화와 항염 효과가 있는 물질에 대한 꾸준한 연구가 지속되어 왔으며, 그에 대한 다양한 해결책이 제시되어 왔는데, 예를 들어 대한민국 특허 공개 번호 제2016-0018496호(특허문헌 1)에서는 항산화 및 항염증에 효능이 있는 천연 조성물이라는 명칭으로, "당귀미, 소목, 오약, 적작약, 향부자, 도인, 홍화, 감초, 계피를 혼합하여 추출한 항산화 및 항염증에 효능이 있는 천연 조성물로서, 당귀미 100중량부에, 소목 50~80중량부, 오약 50~80중량부, 적작약 50~80중량부, 향부자 50~80중량부, 도인 30~70중량부, 홍화 30~70중량부, 감초 20~50중량부, 계피 20~50중량부를 혼합하여 추출한 항산화 및 항염증에 효능이 있는 천연 조성물"을 개시하고 있으며, 대한민국 특허 공개 번호 제2016-0098569호(특허문헌 2)에서는 양주좀개수염 추출물을 함유하는 항염증 항산화 활성의 약학조성물 및 식품조성물이라는 명칭으로, "양주좀개수염(Eriocaulon decemflorum) 추출물을 함유하는, 항염증 조성물로, 상기 양주좀개수염 추출물은 탄소수 1 내지 5의 알코올, 헥산, 클로로포름, 주정, 물 및 이들의 혼합물로 이루어진 군에서 선택된 어느 하나로 냉침법을 이용해 추출한 것인, 항염증 조성물"을 개시하고 있으며, 대한민국 특허 공개 번호 제2018-0021012호(특허문헌 3)에서는 항산화 및 항염증에 효능이 있는 천연 조성물이라는 명칭으로, "당귀미, 소목, 오약, 적작약, 향부자, 도인, 홍화, 감초, 계피, 달맞이꽃, 버드나무, 탱자열매를 혼합하여 추출한 항산화 및 항염증에 효능이 있는 천연 조성물"을 개시하고 있다.In view of the above points, continuous research on substances having antioxidant and anti-inflammatory effects has been continued from the past, and various solutions have been proposed. For example, Korean Patent Publication No. 2016-0018496 (Patent Document 1 ) is a natural composition with antioxidant and anti-inflammatory effects, and is a natural composition with anti-oxidation and anti-inflammatory effects extracted by mixing Angelica Mi, Somok, Oyak, Red Peony, Hyangbuja, Taoin, Safflower, Licorice, and Cinnamon. , 100 parts by weight of Donggwimi, 50-80 parts by weight of Somak, 50-80 parts by weight of Chinese oatmeal, 50-80 parts by weight of Red peony, 50-80 parts by weight of Chinese porridge, 30-70 parts by weight of Guoin, 30-70 parts by weight of Safflower, A natural composition effective for antioxidant and anti-inflammatory effects extracted by mixing 20 to 50 parts by weight of licorice and 20 to 50 parts by weight of cinnamon" is disclosed, and Korean Patent Publication No. 2016-0098569 (Patent Document 2) Under the name of pharmaceutical composition and food composition of anti-inflammatory antioxidant activity containing the extract, "an anti-inflammatory composition containing an extract of Eriocaulon decemflorum, wherein the extract contains alcohol having 1 to 5 carbon atoms, hexane Discloses an anti-inflammatory composition, which is extracted using a cold precipitation method from one selected from the group consisting of chloroform, alcohol, water, and mixtures thereof, and in Korean Patent Publication No. 2018-0021012 (Patent Document 3), antioxidant And under the name of a natural composition with anti-inflammatory effects, "Antioxidant and anti-inflammatory effect extracted from a mixture of Angelica mia, Somok, Oyak, Red peony, Hyangbuja, Taoin, safflower, licorice, cinnamon, evening primrose, willow, and tangerine fruit. A natural composition with
그러나, 상기한 특허문헌들의 발명이 또한 항산화 및 항염증에 효과를 나타낼 수 있겠지만, 항염증 효능을 나타내면서 세포독성이 없고, 더욱이 항균 효능도 동시에 만족할만한 수준으로 나타낼 수 있는지 알 수 없으며, 따라서 상기한 바와 같이 우수한 항산화 및 항염증에 효과를 나타내면서 인체에 세포독성을 나타내지 않는 천연물질로부터의 유효성분을 추출하고 이로부터 항산화 및 항염증 억제용 조성물을 제공하는 것이 여전히 필요한 실정이다.However, although the inventions of the above patent documents may also exhibit antioxidant and anti-inflammatory effects, it is unknown whether they can exhibit anti-inflammatory effects without cytotoxicity and, moreover, antibacterial effects at a satisfactory level at the same time. As such, it is still necessary to extract active ingredients from natural materials that exhibit excellent antioxidant and anti-inflammatory effects and do not exhibit cytotoxicity to the human body, and to provide a composition for anti-oxidation and anti-inflammatory inhibition therefrom.
이에, 본 발명자 등은 상기한 종래 기술에 있어서의 문제점을 인식하고 이를 해결하기 위해 예의 연구한 결과, 자주천인국, 강화사자발 약쑥, 붉나무 및 삼백초잎 등의 천연물질로부터의 추출물을 특정한 조합으로 포함하는 신규 조성물을 주재로 하는 의료용 제형이 항산화 효과를 유지하면서 염증 반응을 효과적으로 제어하여 항염증 효과를 나타내는 동시에 항균 효능 또한 만족할만한 수준으로 나타낼 수 있음을 밝혀내어 본 발명을 완성하게 되었다.Accordingly, the inventors of the present invention recognized the problems in the prior art and as a result of intensive research to solve them, extracts from natural substances such as japonica cheonginuk, ganghwa sajabal wormwood, red cedar and triticale leaves in a specific combination The present invention was completed by finding that a medical formulation based on the novel composition can exhibit an anti-inflammatory effect by effectively controlling an inflammatory reaction while maintaining an antioxidant effect, and at the same time exhibit an antibacterial effect at a satisfactory level.
따라서, 본 발명은 상기한 종래 기술에 있어서의 기술적 문제점을 감안하여 된 것으로, 본 발명의 주요 목적은 통상적인 항산화 및 항염제의 문제점을 해소하면서 그 효능을 증진시킬 수 있는 항산화 및 항염증 효능을 갖는 조성물 및 그 제조방법을 제공하기 위한 것이다.Therefore, the present invention has been made in view of the technical problems in the prior art, and the main object of the present invention is to have antioxidant and anti-inflammatory effects that can improve the efficacy while solving the problems of conventional antioxidants and anti-inflammatory agents. It is to provide a composition and a manufacturing method thereof.
본 발명의 다른 목적은 상기와 같은 항산화 및 항염증 효능을 갖는 조성물이 인체에 유해한 반응을 일으키지 않으면서 우수한 항산화 및 항염 효능을 나타내도록 하기 위해 이러한 효능을 갖는 천연물질을 선별하고 이를 특정한 조건으로 추출 및 조합함에 의해 환경 친화적인 천연물질 추출물의 혼합물을 주성분으로 하는 항산화 및 항염증 효능을 갖는 조성물 및 그 제조방법을 제공하기 위한 것이다.Another object of the present invention is to select natural substances having antioxidant and anti-inflammatory effects and extract them under specific conditions so that the composition having antioxidant and anti-inflammatory effects exhibits excellent antioxidant and anti-inflammatory effects without causing harmful reactions to the human body. It is to provide a composition having antioxidant and anti-inflammatory effects and a method for producing the same, the main component of which is a mixture of environmentally friendly extracts of natural substances by combining with the present invention.
본 발명의 또 다른 목적은 항산화 및 항염증의 우수한 효과를 나타내는 신규한 조성물의 약학적 용도를 제공하기 위한 것이다.Another object of the present invention is to provide a pharmaceutical use of a novel composition exhibiting excellent antioxidant and anti-inflammatory effects.
본 발명의 또 다른 목적은 상기한 우수한 기능성을 갖는 신규한 약학적 조성물을 보다 용이하게 제조할 수 있는 제조 방법을 제공하기 위한 것이다.Another object of the present invention is to provide a manufacturing method that can more easily prepare a novel pharmaceutical composition having the above excellent functionality.
또한, 본 발명의 목적은 상기 언급한 기술적 과제들로 제한되지 않으며, 이에 부가하여 본 발명은 상기한 명확한 목적 이외에 본 명세서의 전반적인 기술로부터 이 분야의 통상인에 의해 용이하게 도출될 수 있는 다른 목적을 달성함을 그 목적으로 할 수 있다.In addition, the object of the present invention is not limited to the above-mentioned technical problems, and in addition, the present invention provides other objects that can be easily derived by a person skilled in the art from the general description of the present specification in addition to the above-mentioned specific objects. can be aimed at.
상기한 본 발명의 목적은 자주천인국, 강화사자발약쑥, 붉나무 및 삼백초잎 등의 천연물질로부터의 추출물이 항염증 및 항산화 효과를 가지는 것으로 알려져 있음에 착안하여 이로부터 이들 추출물을 다른 천연성분과 특정한 조합으로 혼합한 신규 조성물을 제공하고, 이러한 조성물이 염증 반응을 효과적으로 제어하여 항염증 효과와 항산화 효과를 나타내는 동시에 항균 효능 또한 나타낼 수 있음을 밝혀내어 달성되었다.The object of the present invention described above is to focus on the fact that extracts from natural substances such as Japonica astragalus, Ganghwa lion-footed wormwood, Raspberries and Sambaekcho leaves are known to have anti-inflammatory and antioxidant effects, and from this, these extracts are combined with other natural ingredients. It was achieved by providing a novel composition mixed with, and finding that this composition can effectively control the inflammatory response to exhibit anti-inflammatory and antioxidant effects while also exhibiting antibacterial effects.
상기 목적을 달성하기 위한 본 발명의 천연물질 추출물의 혼합물을 주성분으로 하는 항산화 및 항염증 효능을 갖는 조성물은;In order to achieve the above object, a composition having antioxidant and anti-inflammatory effects containing as a main component a mixture of natural substance extracts of the present invention;
자주천인국(Echinacea purpurea), 붉나무 잎/줄기(Rhus semialata leaf/stem), 삼백초잎(Saururus chinensis leaf) 및 강화사자발약쑥(artemisia princepes)의 추출물로 구성된 것임을 특징으로 한다.It is characterized in that it is composed of extracts of Echinacea purpurea, Rhus semialata leaf/stem, Saururus chinensis leaf, and Artemisia princepes.
본 발명의 다른 구성에 따르면, 상기 조성물은 자주천인국, 붉나무 잎/줄기, 삼백초잎 및 강화사자발약쑥의 추출물을 주성분으로 하며, 여기에 당느릅나무뿌리(유근피, Ulmus Davidiana root), 병풀잎(Centella asicatic leaf), 또는 배초향(Agastache rugosa)의 추출물이 단독으로 또는 조합하여 일정한 비율로 부가되어 구성된 것임을 특징으로 한다.According to another configuration of the present invention, the composition contains as main components extracts of purple astragalus, raspberry leaves/stems, sambaekcho leaves, and ganghwa lion-footed mugwort, here, elm root (Ulmus Davidiana root), Centella asiatica leaf (Centella asicatic leaf), or extracts of Baechohyang (Agastache rugosa) are added alone or in combination at a constant ratio.
본 발명의 또 다른 구성에 따르면, 상기 조성물은 자주천인국, 붉나무 잎/줄기, 삼백초잎, 및 강화사자발약쑥의 추출물을 주성분으로 하며, 여기에 쥐손이풀, 또는 어성초의 추출물이 단독으로 또는 조합하여 일정한 비율로 부가되어 구성된 것임을 특징으로 한다.According to another configuration of the present invention, the composition contains extracts of Japonica chinensis, Raspberry leaves/stems, Sambaekcho leaves, and Ganghwa lion-footed mugwort as main components, wherein the extracts of J. japonica, or Eoseongcho are used alone or in combination at a constant ratio It is characterized in that it is composed by adding.
본 발명의 또 다른 구성에 따르면, 상기 조성물은 자주천인국, 붉나무 잎/줄기, 삼백초잎, 강화사자발약쑥, 당느릅나무뿌리, 병풀잎, 및 배초향의 추출물을 주성분으로 하며, 여기에 쥐손이풀, 또는 어성초의 추출물이 단독으로 또는 조합하여 일정한 비율로 부가되어 구성된 것임을 특징으로 한다.According to another configuration of the present invention, the composition contains extracts of purple astragalus, raspberry leaves / stems, sambaekcho leaves, ganghwa lion's feet wormwood, sugar elm roots, centella leaves, and baechohyang as main components, and here It is characterized in that the extracts are added alone or in combination at a constant ratio.
상기 다른 목적을 달성하기 위한 본 발명의 천연물질 추출물의 혼합물을 주성분으로 하는 항산화 및 항염증 효능을 갖는 조성물의 제조방법은;A method for preparing a composition having antioxidant and anti-inflammatory effects, the main component of which is a mixture of extracts of natural substances of the present invention for achieving the above object;
항산화 및 항염증 효능을 갖는 조성물을 구성하는 자주천인국, 붉나무 잎/줄기, 삼백초잎, 강화사자발약쑥, 당느릅나무뿌리, 병풀잎, 및 배초향 각각을 약 5중량%로 건조시킨 건물로 하여 3차로 증류수를 활용하여 121℃에서 15분 동안 고압멸균 추출하는 단계,The composition having antioxidant and anti-inflammatory effects was dried in about 5% by weight of each of purple acrophytes, rhus leaves/stems, triticale leaves, Ganghwa lion's foot wormwood, sugar elm roots, centella leaves, and pear grass, and dried three times. High-pressure sterilization extraction at 121 ° C. for 15 minutes using distilled water,
추출한 열수 추출물을 원심분리를 하여 상층액만 분리하는 단계, Separating only the supernatant by centrifuging the extracted hot water extract;
상기 단계에서 분리된 각 열수 추출물을 일정한 비율로 조합으로 혼합하는 단계; 및Mixing each of the hot water extracts separated in the above step in a combination at a constant ratio; and
상기 조합된 혼합물을 동결건조하여 분말화하는 단계에 의해 제조함을 특징으로 한다.It is characterized in that it is prepared by lyophilizing the combined mixture to powder.
상기와 같이 구성되는 본 발명의 천연물질 추출물의 혼합물을 주성분으로 하는 항산화 및 항염증 효능을 갖는 조성물은 일반적인 항산화 및 항염증 제제의 문제점을 완전히 해소하면서 인체에 유해한 반응을 일으키지 않고 우수한 항산화 및 항염 효능을 나타내도록 하기 위해 천연물질을 선별하고 이를 특정한 조건으로 추출 및 조합함으로 환경 친화적인 천연물질 추출물의 혼합물을 주성분으로 하여 항산화 및 항염증 효능을 갖는 조성물을 제공하는 유용한 작용효과를 제공할 뿐만 아니라, 이러한 신규 조합으로 되는 약학적 용도를 제공하므로 상기한 종래의 기술에서 요구되는 문제점을 모두 해결하는 유용한 작용효과를 또한 제공한다.The composition having antioxidant and anti-inflammatory effects, which is composed of the mixture of natural substance extracts of the present invention as a main component, completely solves the problems of general antioxidant and anti-inflammatory agents, does not cause harmful reactions to the human body, and has excellent antioxidant and anti-inflammatory effects. By selecting natural substances and extracting and combining them under specific conditions to provide a composition having antioxidant and anti-inflammatory effects using a mixture of environmentally friendly natural substance extracts as a main component, useful effects are provided, Since this novel combination provides a pharmaceutical use, it also provides useful functional effects that solve all the problems required in the prior art.
도 1은 본 발명의 각 실시예에 따른 조성물의 항산화 및 항염증 효능을 나타내는 그래프로, 세로축은 DPPH 소거 활성을 나타낸다.
도 2는 본 발명의 각 실시예에 따른 조성물의 항산화 및 항염증 효능을 나타내는 그래프로, 세로축은 ABTS 소거 활성을 나타낸다.
도 3은 본 발명의 각 실시예에 따른 조성물의 NO 소거능은 도시하는 그래프로, 세로축은 아질산염의 농도를 나타낸다.Figure 1 is a graph showing the antioxidant and anti-inflammatory efficacy of the composition according to each embodiment of the present invention, the vertical axis represents the DPPH scavenging activity.
Figure 2 is a graph showing the antioxidant and anti-inflammatory efficacy of the composition according to each embodiment of the present invention, the vertical axis represents the ABTS scavenging activity.
Figure 3 is a graph showing the NO scavenging ability of the composition according to each embodiment of the present invention, the vertical axis represents the concentration of nitrite.
이하, 본 발명을 첨부된 도면을 참고로 하여 바람직한 실시형태에 의해 보다 상세히 설명하기로 한다. 하지만, 본 발명의 범주가 여기에 한정되는 것이 아님은 물론이다.Hereinafter, the present invention will be described in more detail by preferred embodiments with reference to the accompanying drawings. However, it goes without saying that the scope of the present invention is not limited thereto.
본 명세서에서, 실시형태는 본 발명의 개시가 완전하도록 하며, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자에게 발명의 범주를 완전하게 알려주기 위해 제공되는 것으로서, 본 발명의 범주는 단지 청구항에 의해 정의될 뿐이다. 따라서, 몇몇 실시형태들에서, 잘 알려진 구성 요소, 잘 알려진 동작 및 잘 알려진 기술들은 본 발명이 모호하게 해석되는 것을 피하기 위하여 구체적으로 설명하지 않는다. In this specification, the embodiments are provided to make the disclosure of the present invention complete, and to completely inform those skilled in the art of the scope of the invention to which the present invention belongs, and the scope of the present invention is limited only to the claims. is defined only by Thus, in some embodiments, well-known components, well-known operations and well-known techniques are not described in detail in order to avoid obscuring the interpretation of the present invention.
본 명세서에서 사용된 용어들은 실시형태를 설명하기 위한 것이며 본 발명을 제한하고자 하는 것은 결코 아니다. 본 명세서에서, 단수형은 문구에서 특별히 언급하지 않은 한 복수형도 포함한다. 또한, '포함(또는, 구비)한다'로 언급된 구성 요소 및 동작은 하나 이상의 다른 구성요소 및 동작의 존재 또는 추가를 배제하지 않는다. The terms used herein are for describing the embodiments and are in no way intended to limit the present invention. In this specification, singular forms also include plural forms unless specifically stated otherwise in a phrase. Also, elements and operations referred to as 'include (or include)' do not exclude the presence or addition of one or more other elements and operations.
청구범위 및 명세서에 사용된 용어들은 달리 명시되지 않는 한, 하기에 기술된 바와 같이 정의된다. 또한, 본 명세서에서 사용되는 임의의 용어 또는 기호가 아래에 제시된 바와 같이 정의되지 않는 경우, 이것은 당해 기술분야에서 그것의 일반적인 의미를 가질 것이다.Terms used in the claims and specification, unless otherwise specified, are defined as set forth below. Also, if any term or symbol used herein is not defined as set forth below, it shall have its ordinary meaning in the art.
본 발명의 실시는 당업계의 기술 내에 있는 유기 화학, 분자 생물학 (재조합 기술을 포함함), 미생물학, 세포 생물학, 생화학 및 면역학의 통상적인 기술의 사용을 포함한다.The practice of the present invention involves the use of conventional techniques of organic chemistry, molecular biology (including recombinant techniques), microbiology, cell biology, biochemistry and immunology that are within the skill of the art.
본 명세서 및 첨부된 청구항들에서 사용된 바와 같이, 요소를 설명하는 맥락에서, 특히 청구범위의 맥락에서, 단수 및 유사한 지시대상은 본 명세서에 달리 나타내지 않는 한 또는 문맥상 분명히 반대로 되지 않는 한 단수 및 복수 양자를 포함하는 것으로 해석되어야 한다. 본 명세서에서 값의 범위의 언급은 단지 본 명세서에서 다르게 지시되지 않는 한, 범위의 상한 및 하한을 포함하여, 범위 내에 속하는 각각의 개별 값을 개별적으로 지칭하는 속기 방법으로서 기능을 하기 위한 것이며, 각각의 개별 값은 본 명세서에서 개별적으로 인용된 것처럼 본 명세서에 포함된다. 본 명세서에서 기재된 모든 방법은 본 명세서에서 달리 지시되거나 문맥상 명백하게 반대되지 않는 한 임의의 적합한 순서로 수행될 수 있다.As used in this specification and the appended claims, in the context of describing elements, particularly in the context of the claims, the singular and similar referents refer to the singular and the singular, unless indicated otherwise in the specification or the context clearly dictates the contrary. It should be construed as including both plural. Recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each individual value falling within the range, inclusive of the upper and lower limits of the range, unless otherwise indicated herein, and each Individual values of are incorporated herein as if individually recited herein. All methods described herein can be performed in any suitable order unless otherwise indicated herein or clearly contradicted by context.
본 발명의 바람직한 실시형태에 따르면, 본 발명의 천연물질 추출물의 혼합물을 주성분으로 하는 항산화 및 항염증 효능을 갖는 조성물은;According to a preferred embodiment of the present invention, a composition having antioxidant and anti-inflammatory effects comprising a mixture of natural substance extracts of the present invention as a main component;
자주천인국(Echinacea purpurea), 붉나무 잎/줄기(Rhus semialata leaf/stem), 삼백초잎(Saururus chinensis leaf) 및 강화사자발약쑥(artemisia princepes)의 추출물로 구성된다.It is composed of extracts of Echinacea purpurea, Rhus semialata leaf/stem, Saururus chinensis leaf, and Artemisia princepes.
본 발명의 바람직한 실시형태에 따르면, 본 발명의 천연물질 추출물의 혼합물을 주성분으로 하는 항산화 및 항염증 효능을 갖는 조성물은;According to a preferred embodiment of the present invention, a composition having antioxidant and anti-inflammatory effects comprising a mixture of natural substance extracts of the present invention as a main component;
자주천인국, 붉나무 잎/줄기, 삼백초잎 및 강화사자발약쑥의 추출물을 주성분으로 하며, 여기에 당느릅나무뿌리(유근피, Ulmus Davidiana root), 병풀잎(Centella asicatic leaf), 또는 배초향(Agastache rugosa)의 추출물이 단독으로 또는 조합하여 일정한 비율로 부가되어 구성될 수 있다.The main ingredients are extracts of Japonica chinensis, red tree leaves/stems, Sambaekcho leaves, and Ganghwa lion-footed mugwort, and the extracts of sugar elm root (Ugeunpi, Ulmus Davidiana root), Centella asicatic leaf, or Baechohyang (Agastache rugosa) are added as main ingredients. Extracts may be added alone or in combination at a constant ratio.
본 발명의 바람직한 실시형태에 따르면, 본 발명의 천연물질 추출물의 혼합물을 주성분으로 하는 항산화 및 항염증 효능을 갖는 조성물은;According to a preferred embodiment of the present invention, a composition having antioxidant and anti-inflammatory effects comprising a mixture of natural substance extracts of the present invention as a main component;
자주천인국, 붉나무 잎/줄기, 삼백초잎, 및 강화사자발약쑥의 추출물을 주성분으로 하며, 여기에 쥐손이풀, 또는 어성초의 추출물이 단독으로 또는 조합하여 일정한 비율로 부가되어 구성될 수 있다.It is composed of extracts of Japonica chinensis, red tree leaves/stems, Sambaekcho leaves, and Ganghwa lion-footed mugwort as main components, and extracts of J. japonica or Houttuynia cordata may be added alone or in combination at a constant ratio.
본 발명의 바람직한 실시형태에 따르면, 본 발명의 천연물질 추출물의 혼합물을 주성분으로 하는 항산화 및 항염증 효능을 갖는 조성물은;According to a preferred embodiment of the present invention, a composition having antioxidant and anti-inflammatory effects comprising a mixture of natural substance extracts of the present invention as a main component;
자주천인국, 붉나무 잎/줄기, 삼백초잎, 강화사자발약쑥, 당느릅나무뿌리, 병풀잎, 및 배초향의 추출물을 주성분으로 하며, 여기에 쥐손이풀, 또는 어성초의 추출물이 단독으로 또는 조합하여 일정한 비율로 부가되어 구성될 수 있다.The main ingredients are extracts of purple cheonginguk, red tree leaves/stems, sambaekcho leaves, ganghwa lion-footed mugwort, elm root, centella leaf, and baechohyang, and extracts of jwisonipul or eoseongcho are added alone or in combination at a certain ratio. can be configured.
본 발명의 바람직한 실시형태에 따라 항산화 및 항염증 효능을 갖는 조성물을 구성하는 자주천인국, 붉나무 잎/줄기, 삼백초잎, 강화사자발약쑥, 당느릅나무뿌리, 병풀잎, 및 배초향의 추출물은 당업계에 알려진 통상적인 방법을 사용하여 얻을 수 있다. 보다 구체적으로, 이들 자주천인국, 붉나무 잎/줄기, 삼백초잎, 강화사자발약쑥, 당느릅나무뿌리, 병풀잎, 및 배초향의 활성 성분을 추출하는 방법은 바람직하게는 열수 추출법 이용하여 얻을 수 있다.According to a preferred embodiment of the present invention, the extracts of purple acrophytes, raspberry leaves/stems, triticale leaves, ganghwa lion's foot wormwood, sugar elm roots, centella asiatica leaves, and baechohyang constituting a composition having antioxidant and anti-inflammatory effects are in the art It can be obtained using known conventional methods. More specifically, the method of extracting the active ingredients of these purple acrophytes, red tree leaves/stems, three baekcho leaves, Ganghwa lion's foot wormwood, sugar elm root, centella leaf, and baechohyang is preferably obtained using a hot water extraction method.
보다 구체적으로는, 본 발명의 바람직한 실시형태에 따라 사용되는 자주천인국, 붉나무 잎/줄기, 삼백초잎, 강화사자발약쑥, 당느릅나무뿌리, 병풀잎, 및 배초향의 추출은 상기 각 천연물질을 약 5중량%로 건조시킨 건물을 3차로 증류수를 활용하여 121℃에서 15분 동안 고압멸균 추출할 수 있다. 이와 같이 추출한 열수 추출물은 원심분리를 하여 상층액만 분리한다. 그런 다음 각 열수 추출물을 원하는 여러 조합으로 혼합하여 동결건조하여 분말화할 수 있다.More specifically, the extraction of purple celestial ginseng, raspberry leaf/stem, triticale leaf, ganghwa lion's foot wormwood, sugar elm root, centella leaf, and baechohyang used according to a preferred embodiment of the present invention is about 5 The dry matter by weight % can be extracted by high-pressure sterilization at 121 ° C. for 15 minutes using distilled water for the third time. The hot water extract extracted in this way is centrifuged to separate only the supernatant. Then, each hot water extract can be mixed in desired combinations and lyophilized to powder.
상기와 같이 동결건조된 분말이 본 발명의 바람직한 실시형태에 따른 조성물을 제조하는데 사용될 수 있다.The lyophilized powder as described above may be used to prepare a composition according to a preferred embodiment of the present invention.
또한, 본 발명의 바람직한 실시형태에 따라 사용되는 쥐손이풀, 또는 어성초의 추출도 상기와 같은 자주천인국, 붉나무 잎/줄기, 삼백초잎, 강화사자발약쑥, 당느릅나무뿌리, 병풀잎, 및 배초향을 추출하는 방법을 사용하여 수행될 수 있다.In addition, the extraction of rattlesnake or eoseongcho used according to a preferred embodiment of the present invention also extracts the above-mentioned purple cheonginguk, raspberry leaves / stems, triticale leaves, ganghwa lion's foot wormwood, elm root, centella leaf, and baecho scent method can be performed.
본 발명의 바람직한 실시형태에 따라 사용되는 자주천인국은 항산화, 항균 및 항염 효능을 가진다. 자주천인국의 항산화, 항염, 저혈당, 세포 증식 억제 효능을 가지는데, 이는 문헌 "Studies on phytochemical, antioxidant, anti-inflammatory, hypoglycaemic and antiproliferative activities of Echinacea purpurea and Echinacea angustifolia extracts. Pharm Biol. 2017 Dec;55(1):649-656. doi: 10.1080/13880209.2016.1265989"에 기술되어 있는 바와 같다. 자주천인국은 또한 문헌 "Echinacea purpurea-derived alkylamides exhibit potent anti-inflammatory effects and alleviate clinical symptoms of atopic eczema. J Dermatol Sci. 2017 Oct;88(1):67-77. doi: 10.1016/j.jdermsci.2017.05.015. Epub 2017 May 27"을 참고하면, 강력한 항염 효과로 아토피 습진 증상에 효과를 나타낸다는 것을 알 수 있다.Japonica astragalus used according to a preferred embodiment of the present invention has antioxidant, antibacterial and anti-inflammatory effects. It has antioxidant, anti-inflammatory, hypoglycemic, and cell proliferation inhibitory effects of Japonica astragalus, which is described in the literature "Studies on phytochemical, antioxidant, anti-inflammatory, hypoglycaemic and antiproliferative activities of Echinacea purpurea and Echinacea angustifolia extracts. Pharm Biol. 2017 Dec;55( 1):649-656. doi: 10.1080/13880209.2016.1265989". Jaju Cheoninguk also reported on “Echinacea purpurea-derived alkylamides exhibit potent anti-inflammatory effects and alleviate clinical symptoms of atopic eczema. J Dermatol Sci. 2017 Oct;88(1):67-77. doi: 10.1016/j.jdermsci.2017.05 .015. Epub 2017 May 27", it can be seen that it is effective for atopic eczema symptoms with a strong anti-inflammatory effect.
자주천인국의 추출물에는 다당류가 포함되어 있는데, 이 다당류는 산화적 손상 모델 실험 결과에 따르면 우수한 항산화 효과 보유하고 있는 것으로 나타났다(Polysaccharide from Echinacea purpurea reduce the oxidant stress in vitro and in vivo. Int J Biol Macromol. 2020 Apr 15;149:41-50. doi: 10.1016/j.ijbiomac.2020.01.129. Epub 2020 Jan 14 참고). 자주천인국의 추출물은 NO 생성 억제, NF-kB 신호전달을 통하여 항염 효능을 가지는 것으로 알려져 있으며, 이는 문헌 "Sesquiterpenes from Echinacea purpurea and their anti-inflammatory activities. Phytochemistry. 2020 Nov;179:112503. doi: 10.1016/j.phytochem.2020.112503. Epub 2020 Sep 9"에서 확인할 수 있다.The extract of Japonica japonica contains polysaccharides, which were shown to have excellent antioxidant effects according to the results of oxidative damage model experiments (Polysaccharide from Echinacea purpurea reduce the oxidant stress in vitro and in vivo. Int J Biol Macromol. 2020 Apr 15;149:41-50. doi: 10.1016/j.ijbiomac.2020.01.129. See Epub 2020 Jan 14). Extracts of Japonica japonica are known to have anti-inflammatory effects through inhibition of NO production and NF-kB signaling, which are described in the literature "Sesquiterpenes from Echinacea purpurea and their anti-inflammatory activities. Phytochemistry. 2020 Nov;179:112503. doi: 10.1016 /j.phytochem.2020.112503. Epub 2020 Sep 9".
더욱이, 자주천인국의 추출물에 포함된 다당류는 생체내에서 강력한 항염 특성을 가지고, 다양한 쥐를 사용한 실험에서 쥐의 항균 능력을 향상시키고, 그리고 JNK 활성화를 통하여 대식세포의 식세포 및 세포내 살균 활성을 향상시키는 것으로 나타났다. 이러한 각각의 효능에 대해서는 참고문헌 "Post-screening characterization of an acidic polysaccharide from Echinacea purpurea with potent anti-inflammatory properties in vivo. Food Funct. 2020 Sep 23;11(9):7576-7583. doi: 10.1039/d0fo01367f", "Echinacea purpurea stimulates cellular immunity and anti-bacterial defence independently of the strain of mice. Pol J Vet Sci. 2003;6(3 Suppl):3-5" 및 "Echinacea purpurea Extract Polarizes M1 Macrophages in Murine Bone Marrow-Derived Macrophages Through the Activation of JNK. J Cell Biochem. 2017 Sep;118(9):2664-2671. doi: 10.1002/jcb.25875. Epub 2017 May 15"를 참고한다.Moreover, the polysaccharide contained in the extract of Astragalus japonica has strong anti-inflammatory properties in vivo, improves the antibacterial ability of rats in experiments using various rats, and enhances the phagocytic and intracellular bactericidal activity of macrophages through JNK activation. It has been shown to do For each of these efficacy, reference "Post-screening characterization of an acidic polysaccharide from Echinacea purpurea with potent anti-inflammatory properties in vivo. Food Funct. 2020 Sep 23;11(9):7576-7583. doi: 10.1039/d0fo01367f ", "Echinacea purpurea stimulates cellular immunity and anti-bacterial defense independently of the strain of mice. Pol J Vet Sci. 2003;6(3 Suppl):3-5" and "Echinacea purpurea Extract Polarizes M1 Macrophages in Murine Bone Marrow- Derived Macrophages Through the Activation of JNK. J Cell Biochem. 2017 Sep;118(9):2664-2671. doi: 10.1002/jcb.25875. Epub 2017 May 15".
본 발명의 바람직한 실시형태에 따라 사용되는 강화사자발약쑥은 염증성 T-세포의 증식과 염증 과정을 억제하여 항염 효능(Anti-inflammatory effects of Artemisia princeps in antigen-stimulated T cells and regulatory T cells. J Pharm Pharmacol. 2009 Aug;61(8):1043-50. doi: 10.1211/jpp/61.08.0008 참고), 간암세포에서 항산화 및 항염 효과(Antioxidant and anticancer activity of Artemisia princeps var. orientalis extract in HepG2 and Hep3B hepatocellular carcinoma cells. Chin J Cancer Res. 2013 Oct;25(5):536-43. doi: 10.3978/j.issn.1000-9604.2013.10.02 참고), 실험실내 항산화 및 항염 활성(In vitro antioxidant and anti-inflammatory activities of Jaceosidin from Artemisia princeps Pampanini cv. Sajabal. Arch Pharm Res. 2008 Apr;31(4):429-37. doi: 10.1007/s12272-001-1175-8. Epub 2008 May 1 참고) 등의 효과가 확인되었다.Ganghwa Lion's Foot mugwort used according to a preferred embodiment of the present invention inhibits the proliferation of inflammatory T-cells and the inflammatory process, thereby exhibiting anti-inflammatory effects (Anti-inflammatory effects of Artemisia princeps antigen in-stimulated T cells and regulatory T cells. J Pharm Pharmacol 2009 Aug;61(8):1043-50. doi: 10.1211/jpp/61.08.0008 reference), Antioxidant and anticancer activity of Artemisia princeps var. orientalis extract in HepG2 and Hep3B hepatocellular carcinoma cells.Chin J Cancer Res. 2013 Oct;25(5):536-43.doi: 10.3978/j.issn.1000-9604.2013.10.02), In vitro antioxidant and anti-inflammatory activities of Jaceosidin from Artemisia princeps Pampanini cv. Sajabal. Arch Pharm Res. 2008 Apr;31(4):429-37. doi: 10.1007/s12272-001-1175-8. Refer to Epub 2008 May 1). .
또한 본 발명의 바람직한 실시형태에 따라 사용되는 강화사자발약쑥은 스트렙토코커스 세균의 성장과 생체막(biofilm) 형성의 억제(Artemisia princeps Inhibits Growth, Biofilm Formation, and Virulence Factor Expression of Streptococcus mutans. J Med Food. 2019 Jun;22(6):623-630. doi: 10.1089/jmf.2018.4304. Epub 2019 Apr 25 참고), 항산화 및 항응고 활성(Antioxidant and anticoagulant activities of Ganghwa medicinal mugwort (Artemisia princeps Pampanini) extract Journal of applied biological chemistry v.63 no.4 ,2020, pp.439 - 442 참고) 및 사람의 소장에 기생하는 유해세균에 대한 항염 활성 보유(Growth-inhibiting effects of seco-tanapartholides identified in Artemisia princeps var. orientalis whole plant on human intestinal bacteria. J Appl Microbiol. 2003;95(1):7-12. doi: 10.1046/j.1365-2672.2003.01998.x 참고)의 효과가 밝혀졌다.In addition, the strengthened lion's foot mugwort used according to a preferred embodiment of the present invention inhibits the growth of Streptococcus bacteria and biofilm formation (Artemisia princeps Inhibits Growth, Biofilm Formation, and Virulence Factor Expression of Streptococcus mutans. J Med Food. 2019 Jun;22(6):623-630. doi: 10.1089/jmf.2018.4304. See Epub 2019 Apr 25), Antioxidant and anticoagulant activities of Ganghwa medicinal mugwort (Artemisia princeps Pampanini) extract Journal of applied biological chemistry v.63 no.4 ,2020, pp.439 - 442) and has anti-inflammatory activity against harmful bacteria parasitic on the human small intestine (Growth-inhibiting effects of seco-tanapartholides identified in Artemisia princeps var. orientalis whole plant on human intestinal bacteria. J Appl Microbiol. 2003;95(1):7-12. doi: 10.1046/j.1365-2672.2003.01998.x) was found.
본 발명의 바람직한 실시형태에 따라 사용되는 붉나무 잎/줄기는 항산화 효능을 가진다(Antioxidant properties, physico-chemical characteristics and proximate composition of five wild fruits of Manipur, India J Food Sci Technol. 2015 Feb;52(2):894-902. doi: 10.1007/s13197-013-1128-2. Epub 2013 Aug 9 참고)The raspberry leaves/stems used according to a preferred embodiment of the present invention have antioxidant properties (Antioxidant properties, physico-chemical characteristics and proximate composition of five wild fruits of Manipur, India J Food Sci Technol. 2015 Feb;52(2) :894-902. doi: 10.1007/s13197-013-1128-2. See Epub 2013 Aug 9)
본 발명의 바람직한 실시형태에 따라 사용되는 삼백초잎은 Nrf2/HO-1 활성 억제를 통한 항염 활성을 가진다(Lignans from Saururus chinensis exhibit anti-inflammatory activity by influencing the Nrf2/HO-1 activation pathway. Arch Pharm Res. 2019 Apr;42(4):332-343. doi: 10.1007/s12272-018-1093-4. Epub 2019 Jan 4 참고). 또한 삼백초잎은 쥐 실험에서 대식세포에서의 항염 효과를 증명하였다(Anti-inflammatory effects of Saururus chinensis aerial parts in murine macrophages via induction of heme oxygenase-1. Meng X, Kim I, Jeong YJ, Cho YM, Kang SC. Exp Biol Med (Maywood). 2016 Feb;241(4):396-408. doi: 10.1177/1535370215614657. Epub 2015 Nov 8 참고). 또한 삼백초잎은 산화적 스트레스로부터 간을 보호하는 효능(Protection of Saururus Chinensis Extract against Liver Oxidative Stress in Rats of Triton WR-1339-induced Hyperlipidemia. Toxicol Res. 2014 Dec;30(4):291-6. doi: 10.5487/TR.2014.30.4.291 참고), 항산화 및 항염 효능(Antioxidative and anti-inflammatory effects of Saururus chinensis methanol extract in RAW 264.7 macrophages. J Med Food. 2005 Summer;8(2):190-7. doi: 10.1089/jmf.2005.8.190 참고), 및 추출물에 포함된 단일 성분이 항균, 항산화, 항염 활성 보유(meso-Dihydroguaiaretic acid attenuates airway inflammation and mucus hypersecretion in an ovalbumin-induced murine model of asthma. Int Immunopharmacol. 2016 Feb;31:239-47. doi: 10.1016/j.intimp.2015.12.033. Epub 2016 Jan 11 참고)을 가지고, 또한 삼백초잎 추출물은 항염, 항혈관신생, 항통각 활성을 가지고(Anti-inflammatory, anti-angiogenic and anti-nociceptive activities of Saururus chinensis extract. J Ethnopharmacol. 2008 Nov 20;120(2):282-6. doi: 10.1016/j.jep.2008.08.016. Epub 2008 Aug 23 참고), 삼백초 추출물의 항균성과 두피에 미치는 영향에 대한 연구에서 그 추출물은 항균 활성을 가지는 것으로 나타났다.Sambaekcho leaves used according to a preferred embodiment of the present invention have anti-inflammatory activity through inhibition of Nrf2 / HO-1 activity (Lignans from Saururus chinensis exhibit anti-inflammatory activity by influencing the Nrf2 / HO-1 activation pathway. Arch Pharm Res 2019 Apr;42(4):332-343.doi: 10.1007/s12272-018-1093-4. See Epub 2019 Jan 4). In addition, sambaekcho leaves demonstrated anti-inflammatory effects in macrophages in rat experiments (Anti-inflammatory effects of Saururus chinensis aerial parts in murine macrophages via induction of heme oxygenase-1. Meng X, Kim I, Jeong YJ, Cho YM, Kang See SC.Exp Biol Med (Maywood).2016 Feb;241(4):396-408.doi: 10.1177/1535370215614657. Epub 2015 Nov 8). In addition, the effect of saururus chinensis extract against liver oxidative stress in rats of Triton WR-1339-induced Hyperlipidemia. Toxicol Res. 2014 Dec;30(4):291-6. doi : 10.5487/TR.2014.30.4.291), Antioxidative and anti-inflammatory effects of Saururus chinensis methanol extract in RAW 264.7 macrophages. J Med Food. 2005 Summer;8(2):190-7. doi: 10.1089/jmf.2005.8.190), and a single component contained in the extract has antibacterial, antioxidant, and anti-inflammatory activities (meso-Dihydroguaiaretic acid attenuates airway inflammation and mucus hypersecretion in an ovalbumin-induced murine model of asthma. Int Immunopharmacol. 2016 Feb;31:239-47. doi: 10.1016/j.intimp.2015.12.033. See Epub 2016 Jan 11), and also, the extract of three hundred plants has anti-inflammatory, anti-angiogenic, and anti-nociceptive activities (Anti-inflammatory, anti-angiogenic and anti-nociceptive activities of Saururus chinensis extract. J Ethnopharmacol. 2008
실시예Example
이하, 본 발명을 특정한 실시예에 의해 보다 자세히 설명하지만, 본 발명의 범주가 이들 실시에에 제한되는 것이 아님은 물론이다.Hereinafter, the present invention will be described in more detail with specific examples, but the scope of the present invention is, of course, not limited to these examples.
실시예 1 내지 6Examples 1 to 6
실시예 1 내지 6에서는 자주천인국, 붉나무 잎/줄기, 삼백초잎, 강화사자발약쑥, 당느릅나무뿌리, 병풀잎, 및 배초향를 각각 약 5중량%로 건조시킨 건물을 3차로 증류수를 활용하여 121℃에서 15분 동안 고압멸균 추출하였다.In Examples 1 to 6, the dried ginseng chrysanthemum, red tree leaves/stems, triticale leaves, ganghwa lion's foot wormwood, elm root, centella leaf, and baecho scent were each dried at about 5% by weight at 121 ° C. using distilled water for the third time. Autoclaved extraction was performed for 15 minutes.
상기와 같이 추출한 열수 추출물은 원심분리를 하여 상층액만 분리하였다. 그런 다음 각 열수 추출물을 하기 표 1에 나타난 조합으로 혼합하여 실시예 1 내지 6의 조성물을 제조하였다.The hot water extract extracted as described above was centrifuged to separate only the supernatant. Then, the compositions of Examples 1 to 6 were prepared by mixing each of the hot water extracts in the combinations shown in Table 1 below.
실험예Experimental example
실험예 1. 항산화 효능Experimental Example 1. Antioxidant efficacy
상기 실시예 1 내지 6에 따라 제조된 조성물의 항산화능을 확인하는 실험을 수행하여 하기 표 2와 도 1 및 2에 각각 나타냈다.Experiments were performed to confirm the antioxidant ability of the compositions prepared according to Examples 1 to 6, and are shown in Table 2 and FIGS. 1 and 2, respectively.
실험예 2. 항균 효능Experimental Example 2. Antibacterial efficacy
항균 실험에서 효능을 명확히 나타내는 데이터를 구체적으로 제시하지 않았지만, 대장균, 황색포도상구균, 녹농균 등에 대해 항균 효능을 갖는 것으로 추정되었다.Although data clearly showing efficacy in antibacterial experiments were not presented, it was estimated to have antibacterial efficacy against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa.
실험예 3. 항염 효능Experimental Example 3. Anti-inflammatory efficacy
① 세포독성 확인① Confirmation of cytotoxicity
본 발명의 조성물을 구성하는 각 천연물질은 각각이 세포 독성을 나타내지 않는 것으로 상기 본 발명의 실시예에 따른 각 조성물 또한 세포 독성은 없을 것으로 추정된다.Since each natural substance constituting the composition of the present invention does not exhibit cytotoxicity, it is estimated that each composition according to the embodiment of the present invention also has no cytotoxicity.
② 본 발명의 각 실시예에 따른 조성물의 NO 소거능은 도 3에 도시되어 있다. 도 3에서 알 수 있는 바와 같이 본 발명의 각 실시예에 따른 조성물은 NO를 효과적으로 소거하였다.② NO scavenging ability of the composition according to each embodiment of the present invention is shown in FIG. As can be seen in Figure 3, the composition according to each embodiment of the present invention effectively scavenged NO.
이상에서 설명한 바와 같이, 본 발명의 기술적 사상은 바람직한 실시형태에서 구체적으로 기술되었으나, 상기한 실시형태는 그 설명을 위한 것이며, 그 제한을 위한 것이 아님을 주의하여야 한다. 본 발명의 기술사상 범위 내에서 다양한 변형 및 수정이 가능함은 당업자에게 명백한 것이며, 따라서 이러한 변형 및 수정이 첨부된 특허청구범위에 속함은 당연한 것이다.As described above, the technical idea of the present invention has been specifically described in the preferred embodiments, but it should be noted that the above embodiments are for explanation and not for limitation. It is obvious to those skilled in the art that various variations and modifications are possible within the scope of the technical spirit of the present invention, and therefore it is natural that such variations and modifications fall within the scope of the appended claims.
Claims (5)
추출한 열수 추출물을 원심분리를 하여 상층액만 분리하는 단계,
상기 단계에서 분리된 각 열수 추출물을 일정한 비율로 조합으로 혼합하는 단계; 및
상기 조합된 혼합물을 동결건조하여 분말화하는 단계에 의해 제조함을 특징으로, 천연물질 추출물의 혼합물을 주성분으로 하는 항산화 및 항염증 효능을 갖는 조성물의 제조방법.
The composition of the composition having antioxidant and anti-inflammatory effects, including purple acrophytes, rhus leaves/stems, triticale leaves, ganghwa lion's foot wormwood, sugar elm roots, centella leaves, and Chinese cabbage scent, each of which was dried at about 5% by weight. High-pressure sterilization extraction at 121 ° C. for 15 minutes using distilled water,
Separating only the supernatant by centrifuging the extracted hot water extract;
Mixing each of the hot water extracts separated in the above step in a combination at a constant ratio; and
A method for preparing a composition having antioxidant and anti-inflammatory effects, wherein the mixture of natural extracts is used as a main component, characterized in that the combined mixture is prepared by lyophilization to powder.
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| KR102613837B1 (en) | 2023-12-15 |
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