KR20180116818A - A cosmetic base material composition a fermentation products of rubus coreanus using phellinus baumii mycelium and a functional cosmetic composition comprising the same - Google Patents
A cosmetic base material composition a fermentation products of rubus coreanus using phellinus baumii mycelium and a functional cosmetic composition comprising the same Download PDFInfo
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Abstract
Description
The present invention relates to a mycelium of Phellinus species The present invention relates to a cosmetic raw material composition containing Rubus coreanus fermented by Baumii Mycelium and a functional cosmetic composition containing the same, and more particularly, to a cosmetic raw material composition containing a fermented product obtained by inoculating and culturing a mycelium culture medium And a functional cosmetic composition for skin conditioning or scalp care comprising the cosmetic raw material composition as an active ingredient.
Skin is a part of the body that is directly exposed to the external environment. It is exposed to ultraviolet rays caused by exposure to sunlight in daily life, air pollutants such as yellow dust or automobile soot, and various bacteria, thereby causing skin trouble and irritation. . Such aging of the skin reduces the elasticity of the skin on the appearance of the skin to form wrinkles or to form spots and senile spots to cause skin pigmentation.
In general, aging of the skin is accompanied by various theories such as somatic mutation, excessive production of free radicals, autoimmune reaction, accumulation of toxic substances, errors generated during gene replication, transformation of dendritic proteins, And the diseases caused by various diseases such as adult diseases are attributed to reactive oxygen species (ROS) is recognized that the active oxygen species originating from oxygen and nitrogen to control the natural antioxidants to develop active research is progressing have.
The reactive oxygen species, usually known as harmful oxygen, is a lipid peroxide such as peroxide anion radical, hydrogen peroxide, hydroxy radical, peroxidized lipid or free radical generated in the most stable form, It occurs even under normal conditions, but the presence of antioxidant enzymes such as superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and the like inhibit the production of excessive reactive oxygen species. However, when the active oxygen species is abnormally excessive due to stress and external environmental factors and is not eliminated, the oxidative stress due to the free radicals is added to the living body and DNA, RNA, protein, Cell membrane and cell structure damage, cancer, tissue damage, and aging.
Studies on antioxidants capable of controlling or eliminating these active oxygen species have been actively conducted in order to suppress the rapid increase of various diseases and aging in the modern age. Studies on the efficacy of natural antioxidants such as tocopherol, ascorbic acid, carotenoids, flavonoids and glutathione, and their stabilization techniques, and studies on the synthetic antioxidants butylated hydroxytoluene (BHT), butylated hydroxyanisol (BHA), and many other antioxidants have been continuously studied. Among these antioxidants, tocopherol has a relatively low antioxidative effect, and synthetic antioxidants such as BHA and BHT, which are highly effective, have been used in pharmaceuticals and food fields. However, there is an indication of mutagenicity and toxicity, and in the case of ascorbic acid, Development of the obtained derivatives and formulations is progressing, and development of a natural antioxidant material having more stable and effective effect is urgently required.
In order to solve such a problem, many studies have been made on a cosmetic composition using an extract which is relatively low in toxicity, safe and effective for skin, among phytochemicals, It has been required to develop a material which maximizes its efficacy since the effect of the ingredient is limited.
On the other hand, in the case of natural extracts through fermentation, sugar components present in natural products are converted into alcohols as carbon sources in the metabolic process of microorganisms without using harmful organic solvents, so that the active ingredients in natural products, especially flavonoids, Polyphenols, catechins, etc. are present as glycated and stable compounds, and the extractability of the active ingredient is increased through fermentation, and excellent skin efficacy is expected by the active ingredient which is excluded from sugar and activated.
Recently, consumers' perception of mushroom has expanded into not only edible but also medicinal and taste foods, and mushroom extracts and mycelium extracts and mycelial cultures have been found to be effective in the prevention and treatment of various diseases. It has emerged as an important biological resource for the development of functional foods and medicines. About 20,000 species of mushrooms are known worldwide, of which about 2,000 species can be developed for food. Approximately 992 species of mushrooms distributed in Korea are recorded. Among them, about 100 kinds of edible mushrooms and 50 kinds of mushrooms are found. Especially, more than 20 kinds of mushrooms having high toxicity are identified. More than 20 kinds of mushrooms can be cultivated in Korea, and have been proven to have anti-cancer, low cholesterol and low blood sugar.
The mushroom is also called woody mud mushroom. The mushroom has various shapes such as 6-12cm diameter, 2 ~ 10cm thick, semicircular shape, flat shape, round mountain shape, horseshoe shape. The dark brown hairs on the surface are short and densely packed, then disappear and grow to become crisp. In the early days of growth, mud clusters seem to be united, and after they are all grown, they are called tongue because they are out of their stumps in a tree stump. Since 1968, Kiekawa et al. Have been known to have anticancer effects, many studies have been carried out. In addition to the anticancer effects, they exhibit various pharmacological effects such as immunomodulatory effect, antioxidative effect, liver function improving action, hyperlipidemia improving action, and whitening effect. For medicinal purposes, the moon is yellow or light yellow and is characterized by lack of flavor and aroma. Korea, Japan, Australia, and North America.
Also, the bokbunja is a medicinal product made from unripe berries of bokbunja berries of rosacea, and it is named after the yogan is turned upside down when it eats. It strengthens the kidney function and uses it for the 遗精, 夢 精, 尿 尿, etc. It prevents the weakening of sight, lightens the body and darkens the head. It also makes the skin soft and beautiful.
In addition, the bokbunja contains a large amount of polyphenols, and it has been found that there are anti-inflammatory action, antioxidative action, anti-Helicobacter pylori action, anticancer action, inhibition of aging, prevention of arteriosclerosis, prevention of thrombosis and sterilization effect.
Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a skin- The present invention provides a cosmetic raw material composition comprising a fermented product obtained by solid fermentation of a bokbunja suitable for conditioning and scalp care, and a functional cosmetic composition containing the cosmetic raw material composition.
Disclosure of the Invention The present invention has been made to solve the above problems, and it is an object of the present invention to provide a mycelium of Phellinus Rubus by Baumii Mycelium) coreanus ) fermentation product.
The present invention also provides a cosmetic composition comprising the cosmetic raw material composition of the present invention.
The cosmetic composition of the present invention is preferably used for skin conditioning or scalp care.
In addition, the cosmetic composition of the present invention can be used in cosmetics such as soft lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, powder foundation, emulsion foundation, wax foundation, cleansing cream, cleansing foam, cleansing water, , A lotion, a lipstick, a shadow, a hair spray, a hair lotion, a hair gel, a hair wax, a shampoo and a rinse.
The fermented product obtained by fermenting the bokbunja of the present invention with a mushroom is a composition derived from a natural product having no cytotoxicity and is excellent in antioxidative activity, skin aging inhibition, anti-inflammatory activity and anti-dandruff activity, and is useful as a cosmetic raw material composition for skin conditioning and scalp care So it is very useful in the cosmetics industry.
Brief Description of the Drawings Fig. 1 shows a process for producing a bacterium fermented product by Mycelia mycelium of the present invention.
FIG. 2 shows the results of 5α-reductase assay of the bacterium fermented by the mycelia of the present invention of the present invention.
Fig. 3 and Fig. 4 show gene expression patterns of the inflammation-related factors of the berry fruit fermented by the mycelium of the present invention of the present invention.
Fig. 5 is a graph showing inhibitory activity of bacillus pertussis fermented by the mycelium of the present invention against dandruff.
Hereinafter, the present invention will be described in detail.
The inventors of the present invention have conducted intensive studies to develop materials for functional cosmetic compositions using natural products. As a result, it has been found that when the bacterium fermented in a mushroom obtained by inoculating mushroom mycelium into a bokbunja and culturing it according to a solid culture method, Activity, inhibition of skin aging, anti-inflammatory activity and anti-dandruff activity, and suggests that the bacterium fermented product of the present invention can be used as a raw material composition for a functional cosmetic composition.
Therefore, the present invention relates to a method for producing a mycelium of Phellinus ( Rubus coreanus ) fermented by Baumii Mycelium.
The mushroom used in the production of the cosmetic composition for raw materials of the present invention may be long-lived mushroom, and the mushroom may be bramble strawberry.
The mushroom mycelium of the present invention can be produced by seed culture of the mushroom seedlings. As a preferable example, the mushroom mycelium is inoculated on a PDA (Potato Dextrose Agar) medium, and the mushroom mycelium is inoculated at a temperature of 20 to 25 ° C at intervals of 3 to 4 weeks Cultured and maintained. The seed culture is a step of culturing the mycelia mycelium cultured in storage prior to the main culture. In a preferred embodiment, the seed culture can be obtained as a liquid culture by inoculating a seed culture, i.e., a mycelium, into a culture medium for Mycelia matsutake, and then cultivating it by rotary shaking.
The brambler of the present invention means a fruit. The brambles can be sterilized by washing the prepared brambled fruit, dewatered and dried, and various known methods of sterilization can be applied. In a preferred embodiment, the sterilization may be performed at 121 DEG C for 30 minutes in a high pressure sterilization mode.
The method of inoculating the mycelium of the present invention into the sterilized brambles is aseptically inoculated into sterilized brambles of a liquid culture obtained by the above described mushroom culture. For example, 30 to 70 parts by weight of the mushroom culture can be inoculated to 100 parts by weight of the bokbunja.
Preferably, the culturing is carried out at a temperature of 20 to 35 DEG C, a relative humidity of 70 to 90% and a culturing period of 7 to 45 days. As a preferable example, For 20 days at a temperature of 85% relative humidity.
The fermented product of the brambly solid fermented product of the mushroom mycelium is in the form that the mushroom mycelium is completely covered on the surface of the brambler, and after drying and crushing it, an extract of the brambled solid fermentation product of the mushroom mycelium can be produced.
The solvent used for the extraction may be water or lower ethanol, but is preferably hot water extraction. As a preferred embodiment, the extract can be taken by adding hot water of about 5 to 150 times volume to the bran solid fermented product of the dried and crushed mushroom mycelium, shaking it for 1 to 5 hours, and filtering or centrifuging it. The above process may be performed 1 to 5 times, preferably 3 times. The obtained extract is sterilized in the same manner as the above-described sterilization process.
According to the concrete manufacturing process as described above, the bacterium solid fermented product or extract thereof can be produced by the mycelium of the present invention, and it can be applied as a raw material of the functional cosmetic composition described below as a raw material composition for cosmetics.
Accordingly, the present invention provides a cosmetic composition or a functional cosmetic composition containing the cosmetic raw material composition containing the brambled fermented product of the present invention of the present invention.
The term "functional" as used herein means an antioxidant activity, a skin aging inhibition, an anti-inflammatory activity, a dandruff-suppressing activity, and the like.
Accordingly, the cosmetic composition of the present invention is preferably used for skin conditioning or scalp care.
The brambled fermented product of the mushroom mycelium, which is the main component of the cosmetic composition of the present invention, may be contained in an amount of 0.001 to 50% by weight, preferably 0.01 to 20% by weight, more preferably 0.1 To 10% by weight. Appropriate formulation stability can be ensured within such a content range, and desired antioxidative activity, inhibition of skin aging, anti-inflammatory activity and anti-dandruff activity can be expected.
The cosmetic raw material composition of the present invention may further contain known natural extracts or other ingredients for antioxidation, prevention of skin aging and promotion of skin regeneration within the range of not inhibiting the active activity of the present invention, .
In addition, the composition may also contain components commonly added to cosmetic compositions, such as fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, A cosmetic or dermatological composition such as a perfume, a perfume, a surfactant, water, an ionic or nonionic type emulsifier, a filler, a sequestering and chelating agent, a preservative, a vitamin, a barrier, a wetting agent, an essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent May be formulated into a particular formulation, including adjuvants or carriers commonly used in the field of dermatology.
The cosmetic composition of the present invention may be prepared in any form conventionally produced in the art and may be formulated into various forms such as solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, Containing cleansing, oil and spray, but are not limited thereto. More specifically, the present invention relates to a cosmetic composition for cosmetics such as a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a powder foundation, an emulsion foundation, a wax foundation, a cleansing cream, a cleansing foam, a cleansing water, Hair, hair wax, shampoo, rinse, and the like, for example.
When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .
When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component. In particular, in the case of a spray, a mixture of chlorofluorohydrocarbons, Propane / butane or dimethyl ether.
When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.
In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.
When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.
Hereinafter, the present invention will be described in more detail with reference to preferred embodiments. The following examples illustrate one preferred embodiment of the present invention and are not to be construed as limiting the scope of the present invention.
[Example]
1. Bokbunja solids by Mycelium of Situ mushroom Fermented Heat number Extract preparation
Longevity mushroom ( Phellinus baumii ) was cultured using a PDA (Potato Dextrose Agar, Difco, Co., USA) medium at 25 ° C. using subculture strains of Palm Bios Co., Ltd., while being subcultured at intervals of 4 weeks and stored at 4 ° C. Respectively. In a 500 ml flask, 100 ml of mushroom mycelium solid medium (1 cm x 1 cm, 3 pieces) was inoculated with mycelium of long-lived mushroom condition, and then inoculated in a rotary shaking incubator (Daihan Labtech Co.Korea, 25 ± 1 ° C, 130 rpm) The cells were cultured for 7 days and used as liquid seeds.
The bokbunja was purchased from Daegu herb medicine market, and the bokbunja fruit was washed with water to remove impurities and dehydrated. 100 g of bokbunja was placed in a 3 L culture container, sterilized at 121 캜 for 30 minutes and then cooled at room temperature. Were aseptically inoculated into 100 g of sterilized brambles. Fermentation was carried out for 20 days in an incubator at 24 ° C and 85% relative humidity. After fermentation, 10 times volume of hot water was added, and the mixture was shaken for 2 hours and then immersed for 72 hours. After centrifugation at 10,000 rpm for 10 minutes, the supernatant was taken. After repeating this three times, the extract was concentrated under reduced pressure to prepare a powder (Fig. 1).
2. Activity evaluation
2.1. Antioxidant activity evaluation
The antioxidant activity of the brambled solid fermented product by the above prepared mushroom mycelium was evaluated. As a control group, bokbunja and ascorbic acid were used. Specifically, the hydrothermal extract (100 μg / ml) of the bacterium solid fermentation product of the prepared mushroom mycelia was evaluated by measurement of DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging activity and FRAP activity.
For DPPH scavenging ability, 380 μl of 2 × 10 -4 M DPPH solution dissolved in 99.5% ethanol was added to 20 μl of diluted samples at various concentrations, and the mixture was reacted at 37 ° C. for 30 minutes. Then, microplate reader (Asys Hitech, Expert 96, Asys Co., Austria). DPPH free radical scavenging activity was expressed as a percentage as shown in Table 1 below.
The FRAP activity was carried out according to a known method on a hot-water extract (100 μg / ml) of the bran solid fermented product of the prepared mushroom mycelium.
2.2. Evaluation of anti-inflammatory activity
Griess reagent assay was performed to measure anti - inflammatory activity. Concentrations of nitrite in culture were measured by griess reagent. Raw 264.7 cells were cultured in DMEM medium at 5 × 10 3 cells / ml, inoculated on a 12-well plate, and cultured for 24 hours in a 5% CO 2 incubator. Cells were treated with 1 μg / ml LPS and treated with 1, 10, 100 μg / ml of extracts for 1 hour and cultured for 24 hours. After obtaining the supernatant of the culture, it was reacted with the griess reagent, and the absorbance at 540 nm was measured with an ELISA reader, and the NO production rate was expressed as a percentage.
Bokbunja
When the amount of NO produced in the LPS-induced group was 100% based on the RAW 264.7 cells, the results showed that the NO production inhibitory activity was generally exhibited. After fermentation, NO production tended to decrease. However, most of the high concentration samples showed toxicity and the decrease of NO production due to toxicity was suspected. Therefore, cytotoxicity test using MTT assay showed that the amount of NO was decreased by the cytotoxicity before fermentation, but the amount of NO was decreased after fermentation. This means that NO production is directly reduced rather than cytotoxic.
2.3. 5α - reductase assay
5α-Reductase activity was evaluated by directly isolating 5α-reductase from rat prostate. As a specific method, seven-week-old male SD rats were sacrificed to obtain prostate gland, which was homogenized using liquid nitrogen. The homogenized tissue was lysed with cold lysis buffer (20 mM potassium phosphate, pH 6.6, 0.32 M sucrose, 1 mM dithiothreitol (DTT), 0.2 mM phenylmethyl-sulfonylfluoride (PMSF)). The homogenized tissues were placed in triple ratio of 10 mM Tris-HCl buffer (pH 7.0), and the supernatant was harvested by centrifugation. The supernatant was centrifuged again and the intermediate layer was used as 5α-Reductase. The working solution was prepared with 90 μL of 10 mM Tris-HCl buffer (pH 6.0), 50 μL of 20 μM testosterone, 10 μL of 2 mM NADPH, and 50 μL of 100 mg / mL enzyme. The addition amount of the sample was treated with 1% concentration of the total reaction solution to measure the degree of inhibition. The enzyme reaction was incubated at 37 ° C for 1 h, and the absorbance change was measured under UV-spectrophotometric conditions at 340 nm.
As a result of the experiment, O.D. It was confirmed that the enzyme reaction occurred normally due to the change of the value. As a positive control, finasteride was used at a concentration of 10, 100 μM, and about 20% inhibition activity was confirmed at 100 μM. The bokbunja showed high activity even in the raw material, but showed relatively high activity after the mushroom fermentation (Fig. 2).
2.4. Evaluation of Immunological Expression Patterns
RAW 264.7 cells were cultured in DMEM medium at 5 × 10 3 cells / ml, inoculated on a 12-well plate, and cultured in a 5% CO 2 incubator for 24 hours. Cells were treated with 1 μg / ml of LPS and treated with extracts / samples at 0, 1, 10, and 100 μg / ml for 1 hour and cultured for 24 hours. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to confirm the expression of anti-inflammatory factors. DEPC water and ONE-STEP RT-PCR PreMix Kit (Intron, Korea) were used to separate and quantify RNA by treating with TRIzol reagent (Invitrogen Co., Carlsbad, CA, USA) And amplified using a Mastercycler gradient (Eppendorf, Hamburg, Germany). 1% agarose gel was prepared with 1X TAE buffer, and the PCR product corresponding to each primer was mixed with a DNA gel loading solution, and electrophoresis was performed at 50V. The gel was stained with ethidium bromide (EtBr), and the difference in expression was confirmed by UV. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control.
LPS treatment in RAW264.7 cells showed an increase in inflammatory factors TNF-α, COX-2 and IL-1β. On the other hand, it was confirmed that some of the activities were decreased in the cells treated with the bokbunja, and the expression level of the bokbunja solid fermentation product of the mushroom mycelia was further reduced (Figs. 3 and 4).
2.5. Assessment of dandruff inhibitory activity
Dandruff often occurs when the Pityrosporum yeast in the scalp is overproduced by stress and other causes, so its inhibitory activity is evaluated. Pityrosporum ovale strains were purchased from ATCC (ATCC 12078) and used. The strain was cultured in Luria-Bertani broth for 7 days. Luria-Bertani agar was autoclaved and stained in a petri dish to stain the strain cultured. An 8 mm paper disc was placed in the center of the Petri dish, and 20 μl of the sample was added to the plate and incubated at 35 ° C for 3 days. The degree of inhibition was evaluated by measuring the clear zone of the sample against the inhibition of pityrosporum by caliper.
As shown in Fig. 5, the activity of the mycelium of the mushroom mycelium in the case of the brambled fermented product was about twice as high as that of the native brambled product, which can be expected to increase the inhibitory component of the brambler after fermentation.
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2017
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KR20140074263A (en) * | 2014-05-09 | 2014-06-17 | 주식회사 엘지생활건강 | Cosmetic composition having antioxidation activity |
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