KR20180104239A - Cosmetic composition comprising mixture extract of Opuntia ficus-indica and Agave americana - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin soothing or skin moisturizing containing a mixed extract of Opuntia humifusa and Agave americana as an active ingredient. According to the present invention, a mixed extract of Opuntia humifusa and Agave americana may suppress production of iNOS and COX2, which are inflammation-related factors, to calm skin and increase amount of moisture change of skin and to increase moisturizing power of the skin. Such skin soothing and skin moisturizing effects can enhance a skin barrier by generally improving the damaged skin. In addition, by using a component derived from a natural plant, there is no side effect to the skin, so that the cosmetic composition may be safely applied.

Description

[0002] Cosmetic compositions containing extract of Opuntia ficus-indica and Agave americana,

The present invention relates to a cosmetic composition containing a mixed extract of Tzanthorrhizae and agar-agar as an active ingredient, and more specifically to a cosmetic composition for skin moisturizing or skin soothing which contains, as an active ingredient, a mixed extract of Tzanthorrhizae and agave.

The most important function of the epidermis in the outermost skin is to protect against various external stimuli such as chemicals, air pollutants, dry environment, ultraviolet rays, physical and chemical stimulants, It is a protective function to prevent excessive divergence of water in the body. This protective function can be maintained only when the stratum corneum composed of keratinocytes is normally formed. The outermost stratum corneum (horney layer) is formed from keratinocytes and consists of keratinocytes with complete differentiation and a surrounding lipid layer. The keratinocyte is a characteristic cell in which basal cells continuously proliferating in the lowest epidermis undergo a stepwise change in morphology and function and are elevated to the surface of the skin. After a certain period of time, And the new keratinocyte replaces its function. This repetitive sequence of changes is called "epidermal cell differentiation" or "keratinization". During keratinization, keratinocytes form the stratum corneum while producing natural moisturizing factors (NMF) and intercellular lipids (ceramides, cholesterol, fatty acids), making the stratum corneum solid and flexible, As shown in Fig. However, such a stratum corneum can be easily lost due to excessive harshness, environmental factors such as living habits such as bath, dry atmosphere, pollutants, and endogenous diseases such as atopic skin and senile skin, In recent years, there has been a substantial increase in the number of factors that cause skin dryness, and the number of people suffering from it is increasing (J. Invest. Dermatol. 1983; 80: 44-49).

Inflammation is the immune response of the body in response to scars or diseases. Oxidative stresses such as ultraviolet rays, active oxygen, free radicals, etc. activate inflammatory factors and cause aging of various diseases and skin. One of the features of inflammation is an increase in the oxygenation of arachidonic acid metabolized by the cyclooxygenase (COX) pathway, which produces prostaglandins. Prostaglandins are mediators of inflammation. Therefore, COX activity can be inhibited to suppress the inflammatory reaction. There are two forms of COX: COX-1 and COX-2. Since COX-2 appears to play an important role in the progression of inflammation, suppression of COX-2 may be an effective way to reduce inflammation (Prostaglandins Leukot Essent Fatty Acids, 69 (5), pp. 329 -337, 2003, Neurosurgery, 56 (3), pp. 590-604, 2005).

Another powerful inflammatory mediator, nitric oxide synthase (NOS), an enzyme that generates nitric oxide (NO), is known to play an important role in mediating the inflammatory response. In particular, NO, which is rapidly over-produced by various cytokines or external stimulants induced by iNOS (inducible NOS), is known to cause cytotoxicity and various inflammatory responses, and chronic inflammation is associated with an increase in iNOS activity (Miller MJ et al., Mediators of inflammation, 4, pp. 387-396, 1995; Appleton L. et al., Adv. Pharmacol., 35, pp. 27-28, 1996).

Currently, steroid preparations, topical immunosuppressants, topical antibiotics and antihistamines are used as medicines for treating inflammatory skin diseases. However, when the medicinal preparations are used for a long period of time, skin thinning, discoloration of skin color, osteoporosis, arteriosclerosis, And the like. In addition, in the case of skin inflammation, various internal inflammatory signals are involved, and skin barrier weakness exacerbates such inflammation. For the above reasons, the development of a substance for soothing the skin is emphasized by improving inflammatory skin diseases derived from natural products and safe for human body while minimizing side effects.

It is also known as Opuntia ficus-indica or Opuntia humifusa. These millennia and centipedes are commonly used without mixing. It has been known to be effective for constipation, burns, edema, bronchial asthma, sputum, and pertussis in the private sector and has been used to alleviate constipation and ulcers. Currently, it has been shown to inhibit diabetes, maintain the stability of glucose in the body, decrease cholesterol It is used for the purpose of diabetes treatment in Mexico. In addition, the plant has been known to prevent rheumatoid arthritis and osteoporosis, and it contains flavonoids and a large amount of vitamin C, which is also used for anti-aging and skin care (Chinese medicines, The main pole gangmok). In recent years, it has been reported that the components of the TCI are wound healing (Fitoterpia, 2001; 72: 165 ~ 167) and antiinflammatory effects (Fitoterpia, 2001; 72: 288-290).

Agave Americana L. (Agave Americana L.) is an evergreen, multi-year-old plant belonging to the family Asparagus and Agaricus. It is native to Mexico and grows in tropical and subtropical regions. The fleshy leaf runs about 20 ~ 30, and it grows up to 1 ~ 2m in height. In addition to this, tequila is produced by fermentation and distillation of agave sap. In addition, it can be used for liquor production such as pork and mescal, and molasses, industrial alcohol, vinegar and agar-agar can be produced as raw materials. The agave is a name given to the shape of the leaf as 'a dragon-like tongue'. In addition, Korean Patent No. 1571387 relates to a cosmetic composition for skin moisturizing or skin cleansing, wherein a complex extract containing an agar-agar extract has skin moisturizing effect and skin soothing effect.

However, as in the present invention, the skin soothing (inflammation relieving) and skin moisturizing effects of the mixed extract of Tianyansen and Agaricus are not yet known.

Accordingly, the present inventors have made intensive researches to overcome the problems of the prior art. As a result, they have found that a substance which is effective in relieving skin inflammation and capable of soothing the skin and having excellent skin moisturizing effect, In the case of the mixed extract of Tanshin Seeds and Agaricus, it inhibits the expression of inflammatory factors such as iNOS and COX2, and has excellent skin moisturizing effect as well as high stability and safety.

KR 10-1571387 B

 Chinese medicinal herb medicine  Fitoterpia, 2001; 72: 165-167  Fitoterpia, 2001; 72: 288-290

Accordingly, it is a main object of the present invention to provide a cosmetic composition containing an extract of a combination of a milky water extract and an agar-agar mixture, which suppresses the expression of an inflammatory factor such as iNOS and COX2 and is excellent in skin moisturizing effect and high in stability and safety, There is.

According to one aspect of the present invention, there is provided a cosmetic composition comprising as an active ingredient a mixed extract of acorn and agar.

The inventor of the present invention has found that when a substance having a high stability and safety can be used to calm the skin by alleviating inflammation of the skin from natural plants having no adverse effects on the skin and screening substances having excellent skin moisturizing effect, Effect and skin moisturizing effect, and the present invention has been completed.

In the present invention, the extract may be extracted with any solvent for extracting natural plants, preferably water, anhydrous or hydrous ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, Is extracted with at least one solvent selected from the group consisting of chloroform, glycerin, butylene glycol, propylene glycol or a mixed solvent thereof.

In the case of the extracts of the present invention, the extracts of the stem and the fruit and the leaves and the leaves of the agar-agar were added with 1 L of the extraction solvent and then boiled for 3 hours under reflux, The filtrate was taken and added with water to adjust the total volume to 100 ml. The adjusted solution was left at 4 캜 for 6 days, and the resulting precipitates were filtered through 0.45 탆 membrane.

In addition, in case of the mixed extract of the present invention, the water content of the water-soluble extract of the present invention is not less than 3% And heated to obtain a hot-water extract. After the extraction, the filtrate was taken and added with water to make a total volume of 100 ml. The adjusted solution was left at 4 캜 for 6 days, and the resulting precipitates were subjected to 0.45 탆 membrane filtration to obtain a mixed extract of acorns and agar.

In the present invention, the mixed extract has a mixed weight of 1: 5 to 1: 5, preferably 1: 3: 1 to 3, more preferably 1: 2: 1 to 2, .

In the present invention, the mixed extract is characterized by containing 0.01 to 60% by weight based on the total weight of the cosmetic composition.

In the present invention, the cosmetic composition is characterized in that it is for moisturizing the skin.

According to the experimental example of the present invention, the expression of filaggrin, a factor related to moisture retention and drying in the skin, was measured to confirm the skin moisturizing effect. As a result, Was significantly higher than that of the mixed extracts of acorns or agar. In addition, as a result of confirming the skin moisture increase effect through the human body experiment, it was confirmed that the skin moisture increase rate of the present invention was significantly increased compared to the single extracts. These results indicate that the mixed extract of Tzanthorrhizae and Agaricus has a synergistic effect in comparison with the single extracts of the present invention. This shows that the mixed extract increases the expression amount of pilgar green and the water content in the skin, (See Experimental Examples 3 and 4 of the present invention).

In the present invention, the cosmetic composition is characterized in that it is used for skin soothing.

In the present invention, the cosmetic composition is characterized in that the skin is soothed by inhibiting the expression of an inflammatory factor.

According to the experimental example of the present invention, the expression of cyclooxygenase 2 (COX 2) and inducible NO synthase (iNOS), which are inflammation-inducing factors in skin, As a result of the measurement, it was confirmed that the expression levels of COX 2 and iNOS were significantly reduced in the case of the mixed extract of Tzanthorrhizae and Agaricus, than that of the mixed extract of Tzanthorrhoeae or Agaricus. From these results, it can be concluded that the mixed extracts of the present invention have a synergistic effect compared to the single extracts, and that the mixed extracts reduce the expression amount of COX 2 and iNOS than the single extracts, (See Experimental Examples 1 and 2 of the present invention).

In the present invention, the composition may be used as a skin lotion, lotion, moisturizing cream, essence, cleansing foam, hand cream, foundation, makeup base, lipstick, gel, patch, essence, shampoo, rinse, , A body lotion and a body cleanser.

The ingredients contained in the cosmetic composition of the present invention may contain, in addition to the above-mentioned extracts, the components conventionally used in cosmetic compositions, such as stabilizers, solubilizers, vitamins, pigments and fragrances, And a carrier.

As described above, the mixed extract of the present invention of the present invention inhibits the production of iNOS and COX 2, which are inflammation-related factors, so as to calm the skin and increase the amount of moisture change of the skin. Such skin soothing and skin moisturizing effects can enhance the skin barrier by improving the damaged skin as a whole. As well as. By using a component derived from a natural plant, there is no adverse effect on the skin, so that it can be safely applied as a cosmetic composition.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Comparative Example  One: Millennium  Production of single extract

100 g of the perennial plant purchased from Pyeongchang, Korea were added to 1 liter of water, and the mixture was boiled under reflux for 3 hours. The mixture was filtered through a filter paper, and the filtrate was adjusted to 100 ml by adding water. The mixture was allowed to stand at 4 占 폚 for 6 days, and the resulting precipitates were filtered through a 0.45 占 퐉 membrane to obtain a tsunami extract.

Comparative Example  2: Preparation of agar alone extract

100 g of agar-agar purchased from Jeju Resource Plant Research Institute was added and 1 L of water was added. After boiling for 3 hours under reflux, the mixture was filtered through a filter paper. The filtrate was adjusted to 100 ml by adding water. ℃ for 6 days, and the resulting precipitates were filtered through 0.45 ㎛ membrane to obtain agar-agar extract.

Example  One: Millennium  And agar-mixed extract preparation

(100 g) was prepared by mixing 1: 1 of each of the tubercle seed extract obtained in Comparative Example 1 and the agave seed extract obtained in Comparative Example 2, that is, 50 g of the acorn extract and 50 g of the agave seed extract. The herb name, use site, and content are shown in Table 1.

medicinal herbs Scientific name and use area Content (%) Millennium Opuntia ficus-indica / stem, fruit 50% agave Agave Americana / stem, leaf 50%

Experimental Example  One: iNOS  Measurement of active inhibition effect

The activity of iNOS (inducible nitric oxide synthase, inducible nitric oxide synthase) was measured in order to investigate the inhibitory effect of artificially induced inflammation using Raw264.7 cells, a mouse macrophage. 8 × 10 ⁵ cells were suspended in a 10% FBS-DMEM (Fetal Bovine Serum, fetal bovine serum, Dulbecco's Modified Eagle Medium, Gibco) medium without phenol-red and inoculated on a 24-well plate Respectively. One day after the treatment, the extract was cultured for 18 hours, and treated with 1 μg / ml of lipopolysaccharide (LPS, Sigma) for 24 hours. Thereafter, the supernatant was recovered and added to a 96-well plate in an amount equivalent to 100 μl, and the same amount of Griess reagent (Sigma) was added thereto, followed by gentle shaking at room temperature for 10 minutes, and the absorbance was measured at 570 nm. Sodium nitrite was used as a standard product, a calibration curve was prepared, and the amount of nitric oxide produced in the culture solution was determined for each sample. The% yield of each sample was determined as 100% of the amount of nitric oxide in the group treated with lipopolysaccharide. The results were as shown in Table 2 below using the Cheonjangwon-do extract, agar-agar extract, .

sample % Yield Control 6.84 Lipopolysaccharide (LPS) 100 Silkworm extract alone 64.18 Agar alone extract 82.16 Millennium and agar mixed extract 38.78

As a result, as shown in Table 2, it can be seen that the mixed extract of the present invention of the present invention significantly inhibits iNOS activity compared to the extract of Chenyanthus or agar alone.

Experimental Example  2: Measurement of inhibitory effect of COX 2 gene expression

Raw 264.7 cells, a macrophage of mice, were treated with lipopolysaccharide to increase COX 2 (Cyclooxygenase 2) gene expression and then assayed by RT-PCR. 8 × 10 ⁵ cells were suspended in a 10% FBS-DMEM medium (Fetal Bovine Serum, fetal bovine serum, Dulbecco's Modified Eagle Medium, Gibco) and inoculated on a 100 mm dish. After one day, the extract was treated and cultured for 18 hours, followed by treatment with 1 μg / ml lipopolysaccharide (LPS, lipopolysaccharide, Sigma) for 8 hours. Cell culture medium was removed and 1 ml of triazole (Trizol, Invitrogen) was added. RNA was isolated according to Invitrogen 's RNA isolation method and quantified at 260 nm to perform reverse transcription - polymerase chain reaction Respectively. All-in-one RT-PCR kit (Super Bio Inc.) was used for the reverse transcription polymerization, and the experiment was carried out according to the manual of the all-in-one RT-PCR kit. Respectively.

sample COX2 (%) Control group 100 Lipopolysaccharide (LPS) 116.56 Silkworm extract alone 91.21 Agar alone extract 95.97 Millennium and agar mixed extract 61.51

As a result, as shown in Table 3, it can be confirmed that the mixed extract of the present invention of the present invention remarkably inhibits the expression of COX 2 gene more than the extract of the agar or agar alone.

Experimental Example 3: Measurement of skin moisturizing effect by identification of Fillagrin gene

Human keratinocyte (NHK) and fibroblast (NHF) were used to determine the efficacy of the combined extracts of Tzanthorrhizae and Agaricus in skin cells. Fibroblasts were evaluated using a replicative senescence model that induced cell senescence through repeated subculture. In the case of keratinocytes as a gene, pila green was selected as a moisturizing factor. After 24 hours, the cells were collected, washed twice with 10 ml of phosphate buffered saline (PBS), and then treated with Trizol reagent, Invitrogen (Invitrogen) , Carlsbad, CA, USA) was used to isolate total RNA (total RNA) in the cells. The separated RNA was purified once more using a Qiagen RNeasy kit (Qiagen, Valencia, Calif.), And then separated from the above separated RNA using Superscript Reverse Transcriptase (RT) CDNA was synthesized by using RT-PCR kit (Invitrogen, Carlsbad, Calif.) And quantitative analysis of the expression of pILLA green gene was performed by real-time reverse transcription polymerase chain reaction . The gene expression patterns were analyzed using a TaqMan gene expression assay kit (Applied Biosystems, Forster City, CA) of Applied Biosystems, Inc. The results are shown in Table 4 below.

sample Filla green gene Control group One Silkworm extract alone 1.57 Agar alone extract 1.67 Millennium and agar mixed extract 4.68

Experimental Example  4: Through human experiment Skin moisturizing power  Increase effect measurement

In order to investigate the effect of increasing the skin moisturizing power of the mixed extracts of Tilapia thunbergii and Agave sp. By human body experiment, a skin moisture improvement effect test was carried out as follows.

4-1. Skin moisture  Increase effect

The cosmetics prepared in the following formulation examples were selected from 140 women of 20 to 40 years old who had poor skin and were given Formulation Examples 1 to 5 respectively and applied on the face and the first half for 2 days each for 1 month. At this time, the skin dryness was measured by using a moisture meter (CM820 courage Khazaka electronic GmbH, Germany) in a constant temperature and humidity condition (24 ° C, 40% , 2 weeks, and 5 weeks after the treatment. At this time, a group not containing extract as a negative control group and a lipid mixture were used as a positive control group.

4-1-2. Formulation Example (emulsifier type)

Each of the oil phase and water phase was completely dissolved at 70 DEG C and emulsified at 7,000 rpm for 5 minutes to prepare a cream formulation as shown in Table 5 below.

Formulation example 1 contains the mixed extract of the present invention of the present invention and Formulation Example 2 does not contain the extract as a negative control, Formulation Example 3 contains only the extract of Dillanthes primalum alone, Formulation Example 4 contains only the extract of the agar-agar , Formulation Example 5 contains a lipid mixture as a positive control.

ingredient ( content% ) Formulation Example  One Formulation Example  2 Formulation Example  3 Formulation Example  4 Formulation Example  5 Beads wax 2 2 2 2 2 Stearyl alcohol 5 5 5 5 5 Stearic acid 8 8 8 8 8 Squalene 10 10 10 10 10 Propylene glycol monostearate 3 3 3 3 3 Polyoxyethylene ether One One One One One Preservative, antioxidant dose dose dose dose dose glycerin 4 4 4 4 4 Propylene glycol 8 8 8 8 8 Triethylamine One One One One One Sodium diol polyacrylate 0.2 0.2 0.2 0.2 0.2 Purified water To 100 To 100 To 100 To 100 To 100 Silkworm extract alone - - 2 - - Agar alone extract - - 2 - Millennium and agar mixed extract 2 - - - - Lipid mixture - - - - 2

4-1-3. result

As shown in Table 6, skin moisture was increased in both of the extracts of Solanum tuberosum solanum, Agaricus solanum extract, and the mixture of Chenille seeds and Agaricus mixed extracts. Particularly, it was confirmed that the effect of skin-water increase by the combination of the acorn extract and the agar-mixed extract was remarkable.

Remarks Skin moisture increase rate (%) 1 week Two weeks Three weeks Formulation Example 1 68 75 86 Formulation Example 2 9 11 14 Formulation Example 3 39 50 52 Formulation Example 4 43 52 57 Formulation Example 5 41 49 55

Claims (8)

The cosmetic composition according to any one of claims 1 to 3,
The method of claim 1, wherein the extract is selected from the group consisting of water, anhydrous or hydrous ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, benzene, chloroform, glycerin, butylene glycol, propylene glycol, And extracting with one or more selected solvents.
The cosmetic composition according to claim 1, wherein the mixed extract is extracted at a mixing weight ratio of 1: 3 to 1: 3.
The cosmetic composition according to claim 1, wherein the mixed extract is contained in an amount of 0.01 to 60% by weight based on the total weight of the cosmetic composition.
The cosmetic composition according to claim 1, wherein the cosmetic composition is for moisturizing the skin.
The cosmetic composition according to claim 1, wherein the cosmetic composition is a skin-soothing composition.
The cosmetic composition according to claim 1, wherein the cosmetic composition suppresses the expression of an inflammatory factor to thereby calm the skin.
The composition according to claim 1, wherein the composition is at least one selected from the group consisting of lotion, lotion, moisturizing cream, essence, cleansing foam, hand cream, foundation, makeup base, lipstick, gel, patch, essence, shampoo, rinse, Soaps, body lotions, and body cleansers. ≪ RTI ID = 0.0 > 11. < / RTI >