KR20180103217A - A fertilizer composition comprising L-Methionine - Google Patents

Abstract

The present application relates to a fertilizer composition including L-methionine, and more particularly, to a fertilizer composition including L-methionine and at least one component selected from a group consisting of L-lysine, L-threonine, L-tryptophan, L-arginine, L-valine, L-glutamate, and nucleic acid. Since the fertilizer composition of the present application can increase the utilization rate of amino acid in plants by efficiently supplying amino acids to the plants, a fertilizer containing the fertilizer composition of the present application has an effect of remarkably increasing the growth and production of the plants compared to existing fertilizer products.

Description

A fertilizer composition comprising L-methionine,

The present application relates to a fertilizer composition comprising L-methionine, specifically L-Methionine; And L-lysine, L-threonine, L-tryptophan, L-arginine, L-valine, L-Glutamate) and a nucleic acid. The present invention also relates to a fertilizer composition comprising at least one component selected from the group consisting of L-glutamate and L-glutamate.

Amino acids are used in protein formation in plants and are essential for growth. Plants can be used to synthesize amino acids themselves, but because they require a lot of energy, they prefer to absorb amino acids directly from the environment. That is, when the amino acid is directly absorbed, the process of converting the starting material into the amino acid in the plant is omitted, so that the effect on the growth is fast, and the energy consumption is reduced and the physiological activity in the plant is enhanced. Therefore, research and development of a fertilizer containing amino acid itself has been actively carried out. Specifically, Korean Patent Laid-Open No. 10-2001-0021522 (Apr. 20, 2001) discloses an animal amino acid fertilizer containing an animal amino acid obtained from animal hair as a nitrogen source.

However, the conventional amino acid fertilizer is produced by acid or alkali hydrolysis using mainly animal material such as gelatin, leather, blood and hair, etc. In this case, not only a form of amino acid not used by plants is formed, The productivity and efficiency of the product are deteriorated because the amino acid content of the product can not be controlled. In addition, plants are known to use L-form amino acids, but conventional amino acid fertilizers have a problem in that the L-form and the D-form are mixed and the utilization rate and the water absorption rate of plants are low.

On the other hand, nucleic acid has been used as a food additive for enhancing the taste of food, and it has been known that it has an effect of promoting plant growth when used in plants.

Under these circumstances, the present inventors have made intensive efforts to solve the above problems. As a result, they have found that L-methionine and L-lysine, L-threonine, L-tryptophan When a fertilizer composition containing L-arginine, L-valine, L-glutamate and nucleic acid is used, the growth of the crops And the yield can be significantly improved, thereby completing the present application.

One object of the present application is to provide a pharmaceutical composition comprising L-Methionine; And L-lysine, L-threonine, L-tryptophan, L-arginine, L-valine, L-Glutamate) and a nucleic acid. The present invention also provides a fertilizer composition comprising the same.

This will be described in detail as follows. On the other hand, each description and embodiment disclosed in the present application can be applied to each other description and embodiment. That is, all combinations of the various elements disclosed in this application fall within the scope of the present application. Further, the scope of the present application is not limited by the specific description described below.

As an aspect to accomplish the above object, the present application relates to a method for producing L-methionine; And L-lysine, L-threonine, L-tryptophan, L-arginine, L-valine, L-Glutamate) and a nucleic acid. The present invention also provides a fertilizer composition comprising at least one component selected from the group consisting of L-glutamate and L-glutamate.

The term " fertilizer " as used in this application generically refers to materials that fertilize land and promote the growth of vegetation. Fertilizers are nutrients that are added to the soil or plant to maintain or promote the productivity of the soil and to promote the growth of the crop, or indirectly to improve the physical and chemical properties of the soil and improve useful microorganisms, It means a substance that helps growth.

Fertilizer can promote the growth rate and growth rate of crops, and can specifically increase the leaf length, leaf width, fresh weight of the ground, fresh weight of the ground, or total fresh weight of the plant, and can increase the leaf color of the plant. More specifically, the fertilizer can increase the leaf length, leaf width, fresh weight of the ground, fresh weight of the ground, whole fresh weight or leaf color of the crop such as lettuce, pepper and the like.

The term " amino acid fertilizer " in the present application means a fertilizer containing the aforementioned amino acid as a constituent. Conventionally, the amino acid fertilizer contains an amino acid, which is an animal material as a main raw material, and which is difficult to be directly used by plants. On the other hand, the fertilizer composition of the present application can increase the absorption rate and utilization rate of plants to promote growth.

The term "L-amino acid" in the present application means an amino acid representing a stereochemistry at the α-carbon atom of a chiral amino acid, and a fischer projection formula of an α-amino acid means an amino acid at the left side Where the carboxyl group is up and the carbon chain is vertical. In this application, the L-form amino acid can be used as a fertilizer composition, and the absorption rate and utilization rate of amino acid of the plant can be increased as compared with the conventional amino acid fertilizer.

The fertilizer composition of the present application may be characterized as essentially containing L-methionine. Methionine is a kind of α-amino acid containing sulfur, which is one of the essential amino acids for humans. L-methionine means that the methionine is L-form.

L-lysine, L-threonine, L-tryptophan, L-arginine, L-valine, Valine, L-glutamic acid, and nucleic acid, and more specifically, it may comprise at least two components, more specifically, at least three components, more specifically, But is not limited to, at least four components, more particularly at least five components, more specifically at least six components, and most specifically at least seven components.

More specifically, the fertilizer composition of the present application is characterized in that L-methionine, L-lysine, L-threonine, L-tryptophan, L-arginine L-arginine, L-valine, L-glutamate and nucleic acid.

The term " nucleic acid " in the present application means a polymer substance composed of a purine base and a pyrimidine base, sugar, and phosphoric acid, and is known to be an important substance that constitutes a genetic material such as DNA or participates in various metabolic processes in an organism.

The fertilizer composition of the present application may be characterized in that the nucleic acid is contained. Specifically, the nucleic acid is selected from the group consisting of IMP, Disodium 5'-Inosinate, GMP, Disodium 5'- -Guanylate, or a combination thereof.

In the fertilizer composition of the present application, the content of the L-methionine and the at least one of the components may be 20 to 90 parts by weight, specifically 25 to 85 parts by weight, more preferably 30 to 85 parts by weight, To 80 parts by weight, but is not limited thereto.

The fertilizer composition of the present application may also contain L-methionine, L-lysine, L-threonine, L-tryptophan, L-arginine, L- valine, L-

Specifically, the L-methionine may be used in an amount of 1 to 17 parts by weight, the L-lysine may be used in an amount of 8 to 22 parts by weight, the L-threonine may be used in an amount of 2 to 8 parts by weight based on 100 parts by weight of the fertilizer composition, 0.5 to 4 parts by weight of the L-arginine, 1 to 10 parts by weight of the L-arginine, 0.5 to 5 parts by weight of the L-valine, 7 to 20 parts by weight of the L-glutamic acid and 1 to 10 parts by weight of the nucleic acid. ,

More specifically, the L-methionine is 2 to 14 parts by weight, the L-lysine is 10 to 20 parts by weight, the L-threonine is 3 to 6 parts by weight, the L-tryptophan is 1 to 3 parts by weight, 2 to 8 parts by weight of arginine, 1 to 3 parts by weight of L-valine, 9 to 18 parts by weight of L-glutamic acid, and 2 to 8 parts by weight of nucleic acid, but the present invention is not limited thereto.

The fertilizer composition of the present application may contain inorganic salts in addition to the above-mentioned amino acids. Specifically, the inorganic salts may further include potassium ions or potassium salts. Potassium is required for photosynthesis and synthesis of proteins from amino acids, and it is known that when potassium is insufficient, the growth decreases and the color of the leaves becomes thicker.

Specifically, the potassium ion or the potassium salt may be contained in an amount of 3 to 25 parts by weight, more specifically 5 to 20 parts by weight, based on 100 parts by weight of the fertilizer composition, but is not limited thereto.

The fertilizer composition of the present application may contain other substances that can be added to fertilizers in general in the art besides the amino acids mentioned above, and may specifically include a large amount of essential or trace essential elements of plants. And may contain iron, manganese, boron, zinc, copper, molybdenum, chlorine, and the like as a trace element, and may contain at least one element selected from the group consisting of nitrogen, phosphoric acid, potassium, sulfur, calcium or lime, magnesium, But is not limited thereto.

Amino acid fertilizers containing the fertilizer compositions of the present application may be prepared by methods known in the art and may be in the form of liquid fertilizers or solid fertilizers. More specifically, the amino acid fertilizer may be manufactured in the form of a solid fertilizer such as, but not limited to, powder, granular or solid fertilizer.

The present application relates to a fertilizer composition comprising high-purity L-methionine and at least one L-type amino acid or nucleic acid, wherein the fertilizer composition of the present application can efficiently increase the rate of amino acid utilization in a plant by efficiently supplying amino acids to plants , The fertilizer containing the fertilizer composition of the present application has the effect of significantly increasing the growth and yield of the plant than the existing amino acid fertilizer product.

Hereinafter, the present application will be described in more detail by way of examples. However, these embodiments are for illustrative purposes only, and the scope of the present application is not limited to these embodiments.

Example  1: Comparison of effect of fertilizer composition containing amino acid or nucleic acid

(1) Amino acids and nucleic acids used

L-Lysine 79% (w / w)], threonine [L-Threonine 99% (w / w)], tryptophan [L-Tryptophan 98% (w / w)], methionine [L-Methionine 99% L-Valine 98% (w / w)], glutamic acid [L-Glutamic acid 86% (w / w)], [Disodium 5'-Inosinate (50%) and Disodium 5'-Guanylate (50%)] 99% (w / w) powder products were used. The amino acid and nucleic acid were produced by CJ CheilJedang.

(2) Crop cultivation method

In order to test the growth effect of each amino acid and nucleic acid crop, the amino acid and the nucleic acid were treated to cultivate lettuce (cultivar). The basic fertilization management of lettuce was carried out 7 days before the lettuce planting (planting the seedlings raised in the hot spring to the field) on the basis of the fertilizer application criteria of RDA, 10 days and 20 days later. NK 804 was used for the cultivation of roots, while the roots used for fertilizer used fertilizer.

Then, lettuce was formulated for each pot every 1 week. After 7 days, 14 days and 21 days after formaldehyde, amino acids and nucleic acids were added to the leaves to dissolve the fertilizer in water and sprayed on the leaves of the plants. (Table 1). Amino acids and nucleic acids were diluted 500 times with water and treated with 50 ml / week of lettuce, and each treatment was repeated three times. Leaf length, leaf width, leaf color and fresh weight of lettuce were investigated on the 30th day. Leaf length and leaf width were measured by hand. Leaf color was measured with a chlorophyll system (Model: SPAD-502P) of Konica Minolta Co., and live weight was measured by scales after separating the ground part and the under part.

Treatment Basics and smiles Amino acid and nucleic acid fertilization Non-treatment Untreated Untreated Control process Untreated Racing process Lysine foliar fertilization Threonine moiety process Threonine foliar fertilization Tryptophan process Tryptophan foliar fertilization Methionine process Methionine foliar fertilization Arginine process Arginine foliar fertilization Valin Ward process Fertilization Glutamate process Glutamic acid foliar fertilization Nucleic acid globule process Fertility

(3) Results

The results measured according to the above method are shown in Table 2 below.

Treatment Leaf length (cm) Leaf width (cm) Leaf
(SPAD) all
Fresh weight
(g / object) Above ground
Fresh weight
(g / object) Underground
Fresh weight
(g / object) Non-treatment 14.0 5.5 27.6 13.5 12.9 0.67 Control 14.7 7.0 29.1 20.7 19.7 1.03 Racing 15.2 7.5 29.4 23.2 21.7 1.53 Threonine moiety 15.0 7.8 29.5 21.5 20.4 1.10 Tryptophan 15.8 7.7 29.6 22.2 21.1 1.08 Methionine 15.3 7.3 35.7 23.2 22.0 1.15 Arginine 15.8 7.4 29.8 24.7 23.6 1.07 Valin Ward 15.2 7.4 29.7 22.8 21.8 1.04 Glutamate 15.9 7.2 29.4 23.5 22.4 1.09 Nucleic acid globule 15.0 7.4 29.0 21.0 19.9 1.14

As a result, it was confirmed that the leaf length, leaf width and leaf color were increased in the amino acid and nucleic acid treatments compared to the untreated control and control. In particular, the leaf area was highest in glutamic acid, arginine and tryptophan, and leaf width was higher in threonine, tryptophan and lysine, and leaf color was higher in methionine.

In the case of fresh weight of lettuce, amino acid and nucleic acid were increased compared to untreated and control. Above ground was especially high in arginine, methionine and glutamic acid, and the underground was particularly high in lysine, nucleic acid and methionine.

Therefore, it was confirmed that when mixed with lysine, threonine, tryptophan, methionine, arginine, valine, glutamic acid and nucleic acid, it could be used as a fertilizer to increase the leaf length, leaf width, leaf color, whole fresh weight, In addition, methionine is the most effective amino acid in enhancing the commerciality of lettuce in that methionine has the highest leaf color.

Example  2: Comparison of the effect of fertilizer compositions comprising L-methionine and at least one amino acid or nucleic acid

(1) Amino acids and nucleic acids used

The same amino acid and nucleic acid as those described in Example 1 were used.

(2) Crop cultivation method

In order to test the growth effect of the mixed composition of amino acid and nucleic acid on the crops, lettuce (cultivar: Cheongma) was cultivated, fertilization management, crop cultivation method, investigation items and methods were the same as those of Example 1. The treatments contained L-methionine as a base and the components except for one of the remaining amino acids and nucleic acids were included, and the treatment composition composition ratios are shown in Table 3 below.

Treatment Lysine Threonine Tryptophan Methionine Arginine Balin Glutamic acid Nucleic acid Refined glucose 1 treatment 20 6 3 14 8 3 18 8 20 2 treatment 0 6 3 14 8 3 18 8 40 3 treatment 20 0 3 14 8 3 18 8 26 4 treatment 20 6 0 14 8 3 18 8 23 5 treatment 20 6 3 14 0 3 18 8 28 6 treatment 20 6 3 14 8 0 18 8 23 7 treatment 20 6 3 14 8 3 0 8 38 8 treatment 20 6 3 14 8 3 18 0 28

(3) Results

Leaf length, leaf width, leaf color and fresh weight were measured in the same manner as in Example 1 after the amino acids were treated, and the results are shown in Table 4 below.

Treatment Leaf Leaf width Leaf all
Fresh weight Above ground
Fresh weight Underground
Fresh weight (cm) (cm) (SPAD) (g / object) (g / object) (g / object) 1 treatment 16.8 8.2 36.3 27.1 25.4 1.65 2 treatment 15.9 7.9 34.2 24.3 23.1 1.24 3 treatment 16.2 7.8 34.5 23.9 22.4 1.45 4 treatment 15.3 7.7 33.5 23.9 22.6 1.34 5 treatment 14.5 7.4 33.8 23.0 21.6 1.36 6 treatment 15.5 7.9 35.6 25.7 24.2 1.48 7 treatment 15.2 7.5 32.6 23.6 22.5 1.14 8 treatment 16 7.8 34.6 26.1 24.6 1.47

As a result, leaf, leaf width and leaf color showed the highest values in the 1st treatment containing both amino acid and nucleic acid. 2 to 7 treatments excluding one of amino acids or nucleic acids showed a tendency to be lower than those of one treatment. The total weight of fresh lettuce, fresh weight at the ground level and fresh weight at the bottom of the lettuce showed the highest value in the 1 treatment group.

Therefore, when using a fertilizer composition containing six kinds of L-methionine and the remaining amino acids and a nucleic acid, it was confirmed that the plant foliage, leaf width, leaf color, total fresh weight, overground fresh weight and underground fresh weight were both increased.

Example  3: Comparison of the effect of fertilizer composition and general amino acid fertilizer of the present application

(1) Manufacture of amino acid and nucleic acid mixed fertilizer

As a comparative group, an animal-hydrolyzed amino acid fertilizer (Sichuan mianzhu pengfa biochemical co. Ltd., China), which is a conventional commercial product, was used. L-Lysine 79% (w / w)], threonine [L-Threonine 99% (w / w)], tryptophan [L-Tryptophan 98% L-arginine 99% (w / w)], valine [L-Valine 98% (w / w)], glutamic acid [L- Glutamic acid 86% (w / w), 99% (w / w) of nucleic acid [Disodium 5'-Inosinate (50%) and Disodium 5'-Guanylate (50% The amino acid compositions of the fertilizer compositions (experimental group 1 and experimental group 2) and comparative groups of the present application are shown in Table 5

amino acid Experiment 1 Experiment 2 Comparative group Aspartic acid 3.44% Threonine 3.00% 6.00% 1.90% Serine 5.08% Glutamic acid 9.00% 18.00% 3.87% Glycine 4.81% Alanine 2.57% Balin 1.00% 3.00% 1.94% Methionine 2.00% 14.00% 0.21% Isoleucine 1.31% Leucine 1.14% Tyrosine 0.44% Phenylalanine 2.56% Arginine 2.00% 8.00% 2.66% Proline 3.33% Lysine 10.00% 20.00% 0.66% Tryptophan 1.00% 3.00% Nucleic acid 2.00% 8.00% Sum 30.00% 80.00% 35.92%

(2) Crop cultivation method

In order to test the growth effect of the produced mixed fertilizer on the crops, the mixed fertilizer was treated to produce red pepper (variety: rich). The basic fertilization of red pepper was treated 7 days before red pepper, and 40 days and 60 days after red pepper was planted. NK 804 was used for the cultivation of roots, while the roots used for fertilizer used fertilizer. Thereafter, the red pepper was settled in a unit of 100 cm in length and 40 cm in length. After 10 days of formalinization, before flowering (20 days after formal application), after flowering (30 days after formal administration) After 5 days, the mixed fertilizer of amino acid was fertilized 5 times. In the case of foliar fertilization, the total amount of amino acids in each treatment was different. Therefore, 0.2% of the control group, 0.24% of the control group, and 0.09% of the control group 2 were added to the pepper Respectively.

(3) Results

After the mixed fertilizer was treated, the plant height (the length of the top part of the plant), the weight of the fresh weight (weight of the top and bottom parts after harvesting the pepper), the weight of the dried and the dried red pepper were measured.

Test section
Length (cm)
Fresh weight
(g / 5 objects) In building
(g / 5 objects) Dry pepper yield
(kg / 1000 m ² ) Untreated group 99 1711 503 375 Experiment 1 105 2211 664 442 Experiment 2 105 2221 666 443 Comparative group 100 2032 630 420

As a result, the plant height (length of the top part of the plant) of experimental groups 1 and 2 was increased by 6% compared to untreated group (control group without amino acid fertilizer treatment) and by 5% compared with the control group. Fresh weight (weight of the upper and lower parts after harvesting of pepper) was increased by 29% and 30% and 9%, respectively, compared with the untreated group in the experimental groups 1 and 2. The yield of dry red pepper in experimental groups 1 and 2 was 18% higher than that of untreated group and 5% higher than that of control group.

On the other hand, the amino acid content of the mixed fertilizer used in the experimental group 2 was higher than the amino acid content of the mixed fertilizer used in the experimental group 1, but since the amino acid content of the actual pepper was made the same by varying the dilution factor of the fertilizer at the time of foliar application, There was no significant difference between the experimental groups 1 and 2.

Accordingly, it can be seen that the fertilizer composition of the present application can increase the growth and yield of pepper compared to the amino acid-untreated group, and can further increase the growth and yield of the pepper compared to the animal amino acid hydrolyzed fertilizer of the comparative group.

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all aspects and not restrictive. The scope of the present application is to be interpreted as being within the scope of the present application, all changes or modifications derived from the meaning and scope of the appended claims and from their equivalents rather than the detailed description.

Claims (6)

L-Methionine; And
L-lysine, L-threonine, L-tryptophan, L-arginine, L-valine, L-glutamic acid -Glutamate) and a nucleic acid. ≪ RTI ID = 0.0 > 21. < / RTI >
The method according to claim 1,
Wherein said nucleic acid is 5'-Inosinate, 5'-Disodium 5'-Guanylate (GMP), or a combination thereof.
The method according to claim 1,
Wherein the content of the L-methionine and the at least one component is 30 to 80 parts by weight based on 100 parts by weight of the fertilizer composition.
The method according to claim 1,
Wherein the fertilizer composition comprises L-methionine, L-lysine, L-threonine, L-tryptophan, L-arginine, L-valine, L-
Methionine, L-lysine, L-threonine, and L-tryptophan are contained in an amount of 2 to 14 parts by weight, 10 to 20 parts by weight, 3 to 6 parts by weight, and 3 to 6 parts by weight, 2 to 8 parts by weight of the L-arginine, 1 to 3 parts by weight of the L-valine, 9 to 18 parts by weight of the L-glutamic acid and 2 to 8 parts by weight of the nucleic acid.
The method according to claim 1,
Wherein the fertilizer composition further comprises potassium ions or potassium salts.
6. The method of claim 5,
Wherein the potassium ion or potassium salt is included in an amount of 5 to 20 parts by weight based on 100 parts by weight of the fertilizer composition.