KR20070015899A - Preparation method of a fertilizer containing amino acids using Yeasts - Google Patents

Abstract

The present invention is to produce a fertilizer with the amino acid product of the yeast cell digestion through the yeast self-extinguishing reaction using the yeast of sake lees that have been discarded after the liquor production process, the yeast contained in the main membrane generated after the liquor, such as beer and sake Reuse to adjust the solids content to 4-18%, to promote self-extinguishing and to prevent microbial contamination, alcohol 1-9% (v / v), Protease0.01-1% (w / v) After the digestion was completed, the digestion was completed, and the supernatant was obtained by centrifugation. After filtering, KH ₂ PO ₄ was added to the amino acid fertilizer containing various useful substances. It is about.

Yeast, Amino Acid Fertilizer, Autodigestion

Description

Preparation method of amino acid fertilizer using yeast {Preparation method of a fertilizer containing amino acids using Yeasts}

The present invention relates to a method for producing an amino acid fertilizer comprising, as a nitrogen source, amino acids obtained by self-digestion for 24 hours by adding 9% alcohol and 0.2% Protease at 50 ° C. in yeast obtained from sake lees such as beer and sake. Soil absorption form of nitrogen is an organic nitrogen compound, the amino acid fertilizer of the present invention is easily broken down into low-molecular amino acids, so that the plant is easily absorbed and prevents soil contamination, promotes the growth of crops, and increases the resistance of crops to cold damage. This could be widely used for growing crops.

The prior art is in the case of domestic patent No. 0094000, publication date October 23, 1989, Applicant Kaneyugi Gauge, inventors Kaneyugi Gauge "organic fertilizer and feed material manufacturing method" in the fermentation waste liquid to remove the necessary components in Takju It was fermented for 5 days with microorganisms cultured in distilled waste, and Patent No. 0424083, published date 2003. 05. 16, Applicant Park Young-chul, inventor Park Young-chul's "Lactic acid bacteria yeast liquid fertilizer and its manufacturing method" inoculated with yeast Cultured lactic acid fertilizer prepared by aging in a aging tank for 60 days after the culture has a disadvantage compared to the yeast self-extinguishing fertilizer, and furthermore, liquid fertilizer was prepared without completely decomposing yeast by self-extinguishing. Patent No. 0284976, Publication Date 2000. 08. 05, Applicant Lee Ki-sun, Eun-guk Lee, Inventor Lee Ki-sun's "Method of Manufacturing Complex Amino Acid Fertilizer Using Chicken Hair" and Patent No. 0405107 Publication Date October 11, 2002, Applicant Wonpoong The inventors Jin Jin-ok and three others, "fermented amino acid organic fertilizer and fermented amino acid liquid fertilizer and its manufacturing method" is known, but the conventional method for producing amino acid fertilizers by organic hydrolysis of collagen proteins such as leather, bones, hair, etc. There is a disadvantage in that it takes too long to prepare a complex amino acid fertilizer and hydrolyze it with alkali, and in order to cleave protein bonds, it is common to add a reducing agent or a hydrolysis promoting compound to hydrolyze and acid neutralize and then remove insoluble salts. Soil in rural areas has a problem that acidification and deterioration of soil due to excessive fertilization and environmental pollution of chemical fertilizers, the development of excellent raw materials as amino acid fertilizer is required, the present invention is discarded after the production of liquor The present invention relates to the production of high quality amino acid fertilizers in which proteins are broken down to amino acid units by autodigestion using yeast slurries that have been used or inadequately used.

The present invention to solve this problem is to produce a fertilizer as an amino acid product according to the component decomposition through the yeast self-extinguishing reaction using the yeast of sake lees that were generated as by-products in the liquor production process and discarded. Since the amino acid fertilizer prepared as described above is pure organic, it alkalizes the soil, and since it is a pure amino acid, it enhances the intellect of the soil, and is effective in preventing plant growth, pests, and weed generation.

The conventional self-extinguishing method of yeast, which is a by-product of mainstream, adds saline or ethanol to 10-20 (w / v)% of sake yeast and stirs at 50 ° C. to obtain autolysed digested products by the action of enzymes present in yeast cells. In the case of using liquor yeast, since the yeast cells are mostly heat treated after fermentation, protease is used.

The amino acid fertilizer manufacturing method of the present invention is to reuse the yeast contained in the main film generated after the production of liquor, such as beer and sake, and to filter out impurities one time using a 100-120 mesh sieve to remove impurities, the solid content to 12% After the adjustment, 9% (v / v) alcohol and 0.2% Protease (w / v) were added to the reaction solution to promote self-extinguishing and to prevent microbial contamination. Proteins in yeast are broken down into amino acid units and useful components in the cytoplasm are released. After completion of the decomposition, the supernatant was obtained by centrifugation at 5000 rpm for 20 minutes, followed by filtration, followed by KH ₂ PO ₄ It is obtained by dissolving 1-10% (w / v) and mixing with amino acid fertilizer liquid.

The autodigestion digestion solution obtained as described above is used by applying about 16 kinds of amino acids in 43% of liquid fertilizer.

Insufficient supply of nitrogen to crops results in poor overall growth and poor growth, leaves become smaller, chlorophyll production and chloroplast development worsen, resulting in chlorosis yellowing of the entire crop and yellowing from the lower leaves. It usually dies and becomes shorter because of the lack of nitrogen, nitrogen in plants is sent in the form of relatively water-soluble amines and amides. Insufficient amino acids also lead to poor cell wall formation and weak tissues, making it less susceptible to pests, overgrowing foliage, delaying maturation, and decreasing seed yield.

N (nitrogen) as the main tri-fertilizer to increase the production of agricultural products. P (person). The consumption ratio of K (potassium) is used as N: P ₂ 0 ₅ : K ₂ 0 = 55: 20: 25, which is generally consumed by changing farming methods, agricultural structure, grassland area, and increasing consumption of forest fertilizers. There is an increasing need for the development of fertilizer types and forms. KH ₂ PO with phosphoric acid and Kaliwon 52-34 as phosphoric acid and Kaliwon to correct the insufficient chemical components for stabilizing crop productivity and improving agricultural quality together with the nitrate and mineral trace elements in amino acid fertilizer using yeast ₄ Dissolve 1-10% (w / v) and mix into self-digested amino acid fertilizer solution using yeast.

In the form of nitrogen to the plants absorb through their roots are ammonia (NH ₄ ⁺⁾ and nitrate (NO ₃ ^-) and a terminal cancer monitor nitrogen, but vary slightly depending on the type of fertilizer until it changes into a useful nitrate in crops 40 to 50 days have passed since the absorption of the crop is delayed to confirm the change state of the crop due to nitrogen, resulting in an overuse of the fertilizer. The amino acid fertilizer of the present invention is absorbed as a simple organic nitrogen compound.

In addition, the amino acid fertilizer using yeast is a liquid fertilizer, unlike the powdered amino acid fertilizer preparation, it does not take the cost of drying and concentration, and can solve the disadvantage that does not dissolve in water, so it can be used very easily as a fertilizer and economically It can also be said to be very advantageous.

In addition, the 9% alcohol added during yeast self-extinguishing increases the elution of components in the cells during self-extinguishing and reduces the risk of contamination by microorganisms during self-extinguishing and distribution, thereby improving yield and quality.

Yeast has been used for a long time in food fields such as brewing and bakery industry, and is used as a raw material for manufacturing various useful substances such as vitamins, protein supplements, and nucleic acid extracting raw materials. It is known to contain nucleic acids, various vitamins and minerals.

Yeast slurry, a major by-product of liquor production, is mainly known as Saccharomyces cerevisiae, and about 600 strains are known now. It has been a test material. In the food field, it has been used for baking, alcohol production, feed protein, health food raw materials, single cell protein, vitamins and nucleic acid-related substances. Numerous nutrients such as various amino acids, peptides, adenylic acid, guanylic acid, organic acid, lipids, sugars and related nucleic acid polymers in vivo are included, and amino acids, which are important components of the present invention, are analyzed by an amino acid autoanalyzer. g was added to the reaction vial, 5 mL of 6N-HCl containing 0.05% β-mercaptoethanol was added, and then the reaction vial was raised using an aspirator and decomposed at 110 ° C. for 24 hours, followed by amino acid analyzer (PharmaciaBiotech Co, Biochrom 20). ). The amino acid fertilizer prepared by the method of the present invention was analyzed to contain about 43% of 16 amino acids as shown in Table 1 below.

Amino acid type of fertilizer according to the present invention Amino Acid Content (g / 100g) Lysine 3.79 Methionine (Met) 0.38 Tryptophan (Trp) 0.50 Arginine (Arg) 2.20 Histidine (His) 1.10 Isoleucine (Ile) 2.12 Wooshin (Leu) 3.50 Phenylalanine (Phe) 2.22 Threonine (Thr) 2.34 Val 2.47 Glycine 2.12 Cystine 0.50 Tyrosine (Tyr) 1.45 Alanine (Ala) 3.35 Serine 2.71 Aspartic Acid (Asp) 4.77 Glutamic Acid (Glu) 7.24

TABLE 1

As can be seen in Tables 2 and 3, the yeast degradation solution general components, vitamins, and minerals content can be seen that the yeast degradation solution prepared by the method of the present invention contains a good nutrient as an amino acid fertilizer.

TABLE 2

                    General content% protein 45.5 Fat 0.7 fibrin 1.8 Ash 7.5 carbohydrate 38.5

TABLE 3

Vitamin and Mineral Types of Fertilizers According to the Present Invention content Thiamin 89.4mg / kg Riboflavin 34.1mg / kg Nicotinic acid 436.4 mg / kg Cholin 3789mg / kg Folic acid 9.5mg / kg Biotin 0.9mg / kg Pantothenic acid 107.1 mg / kg Pyridoxine 42.4mg / kg Ca 0.13% P 1.46% Fe 0.013% Mg 0.15% K 1.08% Zn 38.7 mg / kg Mn 5.7 mg / kg

Example 1

The yeast slurry obtained after the liquor production was added to 9% (v / v) alcohol and 0.2% (w / v) Protease of the weight of the reaction solution, followed by self-extinguishing for about 24 hours in a reactor maintained at 50 ° C. . After self-extinguishing is complete, centrifugation is carried out, and the supernatant is obtained and filtered to obtain an amino acid fertilizer. Yield was about 75-80% based on the reaction solution. KH ₂ PO ₄ with phosphoric acid and Kali as 52-34 1-10% (w / v) is dissolved and mixed with the fermented amino acid fertilizer liquid using yeast.

Claims (4)

After adjusting the solids content of the yeast slurry contained in the sake produced after the liquor production to 4-18%, preferably 8-12%, 4-96 hours at a temperature of 40-60 ℃, preferably 48-55 ℃ Preferably, a method of preparing amino acid fertilizer by self-extinguishing for 8-48 hours and completing decomposition and then centrifugation to obtain only the supernatant. According to claim 1, Alcohol 1-9% (v / v), Protease 0.01-1% (w / v), preferably alcohol 4-9%, Protease 0.1-0.4% Method for producing amino acid fertilizer, characterized in that the addition The method of claim 1, wherein after the centrifugation, KH ₂ PO ₄ is added to phosphoric acid and Kaliwon. The method of claim 1, wherein the cultured yeast is self-extinguishing in addition to sake lees such as beer, sake, medicinal sake, makgeolli, and the like.