KR20180078781A - Fasting supplement composition - Google Patents

Abstract

The present invention relates to a fasting assistance composition. Specifically, the fasting assistance composition, according to the present invention, reduces levels of obesity, metabolic syndrome, and various inflammatory indicators, while increasing a nutritional index. In addition, the fasting assistance composition also maintains indicators of liver functions, renal functions, and electrolytes within a normal range, and also reduces fatigue and subjective symptoms on discomfort during a fasting period.

Description

{FASTING SUPPLEMENT COMPOSITION}

The present invention relates to a composition for supporting abstinence.

Due to recent rapid economic developments, exposure to various chemical agents and toxic substances such as heavy metals is increasing due to environmental pollution and products containing chemicals. In addition, the number of people suffering from chronic diseases such as overweight, obesity or metabolic syndrome is increasing due to changes in eating habits due to various social and personal factors, excessive calorie intake and insufficient nutrients.

There are diverse ways to improve this, and there is diet using various foods. However, among the ready-to-eat foods used as a dietary supplement, it is common to cause serious problems such as the addition of harmful substances to the human body, the release of not only poisonous substances from the outside of the body but also basic essential elements, thereby weakening the body. In addition, there is a case in which the biased nutrition is ingested, which causes irreversible serious adverse effects on the body.

In order to improve and supplement this, detox therapy that can reduce body weight while positively releasing only toxic substances introduced into the body has recently attracted attention. Detoxotherapy is a type of alternative medicine, which includes fasting, bowel washing, liver washing, coffee enema, nutritional therapy supplementing trace elements such as minerals and vitamins. They are used together to adjuvant therapy or the like (Shin SU et al, J Korean Orient Assoc Study Obes 3 (1):.. 25-35, 2003).

Among them, fasting is one of the oldest detoxification therapies, and it has been widely used since ancient times for purposes such as religious purposes, methods of mental discipline, and healing of diseases. Fasting consciously restricts ingestion of grain during a certain period of time, which leads to rapid changes in the body in a short period of time. Through various clinical studies, the effectiveness of the treatment has been partially identified. Therefore, It is used as a field. The well-known medical effects of fasting include the treatment of obesity, respiratory diseases (rhinitis and asthma), autoimmune diseases (rheumatism), chronic pain syndrome, metabolic syndrome (hypertension, diabetes and hyperlipidemia) and depression, chronic degenerative and inflammatory diseases , Neuroendocrine activation, anti-stress, antioxidant, anti-aging, and autophagy.

In the clinical application of fasting, in order to reduce the disturbance due to the dietary restriction, it is generally divided into four stages of preparatory stage, single stage stage, recovery stage stage and diet regimen, and promotes excretion of body waste during fasting period In order to activate the metabolism, it is accompanied by adjunctive therapies such as bowel washing, hot and cold bathing, bathing, doanjanggong, and aerobic exercise. This series of treatment is classified as 'fasting therapy or pinotherapy' (Fukuda H et al., Stem Cells 24 (3): 763-771, 2006; David R et al., Stem Cells 23 (4): 77-82, 2005). In recent years, it has been recently shown that the effects of treatment such as increase in body fatigue, side effects such as headache, dizziness, vomiting, electrolyte loss, ketosis, In order to maximize the maximum, one can consume some amount of calories such as vegetable enzyme, honey, agar, and grain fermentation in addition to living water during the tableware (250 to 500 kcal), and use the term 'fasting' instead of 'fasting' And a series of treatments related to it are sometimes referred to as 'modified fasting therapy' (Korean Association of Oriental Rehabilitation Medicine, Textbook of oriental rehabilitation medicine, 2nd ed., Seoul: Koonja Publisher, 2005: 376 -84.).

Accordingly, the present inventors have made efforts to find a method for alleviating the side effects of fasting while improving the treatment effect of fasting, and when the fast-cutting supplement composition according to the present invention is consumed during the fasting period, The present invention has been accomplished by confirming that the indicators of obesity, metabolic syndrome and various inflammations are reduced and the nutritional index is increased while minimizing the increase in body fatigue and electrolyte imbalance.

 Shin SU et al., J Korean Orient Assoc. 3 (1): 25-35, 2003  Korean Association of Oriental Rehabilitation Medicine. Text book of oriental rehabilitation medicine. 2nd ed. Seoul: Koonja Publisher. 376-84, 2005

It is an object of the present invention to provide a cutting aid composition comprising a medicinal plant mixture extract, a plum, a maple sap, and a tincture, and a method for producing the composition.

In order to achieve the above object, the present invention provides a cutting aid composition comprising plum, dermis, sine, ginger, ginseng, moonmoon, omija, ginseng, maple sap and morning syrup.

Further, the present invention provides a method for producing a ginseng extract comprising the steps of: 1) obtaining a mixed extract of dermis, sine, ginger and ginseng, ginseng, omija and ginseng; And 2) a step of mixing and mixing the mixture extract of step 1), plum, maple sap, and morning syrup.

In addition, the present invention provides dietary supplements including plums, dermis, sine, ginger, ginseng, moonmoon, omija, ginseng, maple sap, and orchard.

The composition for abstinence abstinence according to the present invention is useful for reducing indicators of obesity, metabolic syndrome and various inflammation, increasing nutritional index, reducing fatigue while keeping the index of liver function, renal function and electrolyte in a normal range, And the like.

Hereinafter, the present invention will be described in detail.

The present invention provides a cutting aid composition comprising plum, dermis, sine, ginger and ginseng, mamdong dong, omija, ginseng, maple sap and morning syrup.

The term " fasting "or" fasting therapy ", as used herein, refers to a therapy that avoids eating or through which the disease is treated and promoted. It can be divided into four stages, usually preparatory, sectional, restorative and dietetic, and it can refer to all the medical treatments performed during the fasting period. In addition, in order to maintain a long-lasting fasting diet, take appropriate amounts of protein, vitamins, minerals, and water, and eat a minimum of carbohydrates.

Plum ( Prunus mune ) is a round nucleus with a diameter of 2 ~ 3 ㎝. It is divided into two kinds according to the degree of ripe. Cheongmyeong and ume (ume) are separated from the shells and seeds of inexperienced chrysanthemum from mid June to early July. It is dried in sunlight to make it black and exhibits various physiological activities. In one room, plum is used as medicinal herb which shows the effects of dryness, cicada, fatigue recovery, sterilization, and antiparasitic effects. Known components are organic acids such as citric acid, malic acid and succinic acid. They contain terpenoids and flavonoids including minerals such as Ca, Na, Mg and P, and antioxidant, anti-fatigue, anti-inflammatory, It is known that there is an effect. In the present invention, the plum was used for the reduction of body fatigue, electrolyte replenishment and detoxification (anti-inflammation, antioxidation, liver function restoration) effects appearing during a single meal.

The plum may be any kind of plum known in the art. Specifically, the plum may be at least one selected from the group consisting of oatsumi, cucumber and sperm yarns, more specifically, sulfur plum. The plum may be used in the form of an extract, a fermentation broth or a juice. In an embodiment of the present invention, the plum may be a fermentation broth. In addition, the plum may be contained in an amount of 5 to 20 parts by weight, specifically 7 to 15 parts by weight, more specifically 10 to 12 parts by weight, based on 100 parts by weight of the total composition of the cutting aid composition.

The dermis (dermis) is a dried dermis of the mandarin orange, which is an evergreen small almond belonging to the oriental herb, and can be used as a medicinal product having a strong aroma about one year after collection. Known main ingredients are essential oil, hesperidin and vitamin C, and it is known that it strengthens the digestive function by strengthening the nourishment and promotes the waste circulation by facilitating the circulation of the digestive juices. Also, Antioxidant activity, antimicrobial activity, immune enhancement activity, lipid metabolism promotion and anti-obesity. In the present invention, the dermis is used for activating the function of the stomach which may be degraded during the epilator, for promoting lipid metabolism and for anti-obesity effect.

The dermis may be included in an amount of 1 to 5 parts by weight, more specifically 2 to 2.5 parts by weight, based on 100 parts by weight of the total composition of the abstinence-assisting composition.

D-camphor, d-borneol, bornyl acetate, and nerolidol are the major components of the ginseng which are known to have removed the husk from the mature fruit of Yangchunsa, Haenam, . Signs in one room are known to activate gastrointestinal function to help digest and calm, and to exhibit antidiabetic, antiallergic, gastrointestinal function, anti-inflammatory and liver protective effects. In the present invention, sine was used to promote gastrointestinal function, which can be degraded during a fasting period, to promote the release and enhance the detoxification (antioxidant, liver protection) effect.

The sine may be included in an amount of 0.5 to 3 parts by weight, more specifically 1.0 to 1.8 parts by weight, based on 100 parts by weight of the total composition of the abstinence-assisting composition.

The above-mentioned ginger (生薑 科) can be a perennial plant of ginger. It is mainly used for edible and medicinal purposes in fruits and roots. Known main constituents include zingiberol, xingiberene, phellandrene, camphene and citral. It is mainly used for cold, fever, headache due to cold ginger, vomiting due to weakening of stomach function, seawater / sputum, cold depression and drug detoxification. Ginger has been used for antiinflammation, antioxidant, body lipid lowering effect, cholesterol biosynthesis Decreased gastrointestinal function, and decreased body temperature. In the present invention, ginger was used for eliminating intrahepatic cholesterol metabolism which may be caused by the restriction of the intake of lipids, inhibiting the body temperature from decreasing in metabolism, and enhancing the detoxification (anti-inflammatory and antioxidant) effects.

The composition according to the present invention may include all kinds of ginger beverages known in the art. Specifically, the ginger may be any one or more selected from the group consisting of ginger, gourd, excess, and turmeric. The ginger may be contained in an amount of 0.5 to 3 parts by weight, more specifically 1.0 to 1.8 parts by weight, based on 100 parts by weight of the total composition of the abstinence-assisting composition.

The above mammon-dong is a perennial plant of lily and can be used mainly as root. These compounds are known to be steroidal glycoside AH, homoisoflavone, ophioside, and kaempferol-3-glucogalactoside. They are mainly used for the treatment of shinhae, genomic, anti-inflammatory, anti-diabetic, autonomic nervous system improvement, , McMundong has been associated with hypoglycemic effects, antiinflammatory, antiallergic, liver function, lipid metabolism, and osteoclast differentiation inhibition. In the present invention, McMurdo was used for suppression of bone mineral density reduction, anti-diabetic, anti-allergic and anti-inflammatory (anti-inflammatory) effects which may be caused by lack of calcium intake during the fasting period.

The gutters may be included in an amount of 2 to 10 parts by weight, more specifically 3 to 5 parts by weight, based on 100 parts by weight of the total composition of the abstinence-assisting composition.

The Schizophyllum (Schizandrae) is a dried whole fruit of Schizophyllum or Schizophyllum, belonging to Magnoliaceae, and may include components such as iszindrin, glutamicin, citrulline, malic acid and citric acid. Asthma is used in the treatment of seawater, asthma and sweating for the purpose of improving immunity and nourishment, and recent studies have shown that Omiza is effective against inflammation, antioxidation, inhibition of osteoclast differentiation, anti-aging and endurance ≪ / RTI > In the present invention, Omija was used for the reduction of body fatigue during fasting period, prevention of osteoporosis by limiting calcium intake and promotion of detoxification (anti-inflammatory, antioxidant) effect.

The above-mentioned omisza may be contained in an amount of 0.5 to 5 parts by weight, more specifically 1 to 2.5 parts by weight, based on 100 parts by weight of the total of the abstraction-aid composition.

The ginseng (ginseng) is a perennial plant of Araliaceae and can be used mainly as root. Saponins are well-known ingredients, and they are mainly used for the weakness of the body, boredom, fatigue, anorexia, vomiting and diarrhea. In addition, recent studies have reported the effects of ginseng on antioxidant, anti-diabetic, anti-cancer, cardiovascular, anti-fatigue, anti-stress, anti-obesity and immune enhancement. In the present invention, ginseng has been used for reduction of body fatigue, metabolic activity during dieting, prevention of immune function depression due to dietary reduction, and improvement of antioxidant effect.

The ginseng may be contained in an amount of 0.5 to 3 parts by weight, more specifically 1.0 to 1.5 parts by weight, based on 100 parts by weight of the total composition of the abstraction-assisting composition.

In addition, prior to preparing the abstinence-aid composition of the present invention, a mixed extract of the medicaments contained in the abstinence-aid composition can be prepared. Specifically, an extract can be obtained by mixing ginseng, dandelion, omija, sane, ginger and ginseng at a ratio of (2 to 4): (1 to 3) :( 0.5 to 2.5): 1: 1: 1, respectively. The extract may be prepared by mixing and extracting the medicinal materials or mixing the respective medicinal material extracts.

In the above method, the mixed extract may be extracted with water, a C ₁ to C ₂ lower alcohol or a mixture thereof as a solvent. As the extraction method, cold extraction, heat extraction, ultrasonic extraction or reflux cooling extraction may be used have. At this time, the extraction time may be 10 to 48 hours and 15 to 30 hours. According to one embodiment of the present invention, the extraction may be performed for 24 hours. The extraction may be repeated three to five times, specifically three times.

In the composition of the present invention, the maple sap may be added to suppress physiological dysfunction such as metabolic dysfunction due to electrolyte shortage caused by restriction of nutrition during the fasting period. The maple sap contains minerals such as manganese, riboflavin, zinc, magnesium, calcium, potassium and sugar, and electrolytes in an abundant manner, so that such side effects can be prevented. At this time, the maple sap solution may be contained in an amount of 15 to 40 parts by weight, specifically 18 to 35 parts by weight, more specifically 20 to 30 parts by weight, based on 100 parts by weight of the total of the cutting aid composition.

The above-mentioned Jojung is a sweet potato made with oligosaccharide as a raw material of rice. It reaches the intestines without digesting from the top and is effective as a food of the beneficial bacteria to improve the intestinal environment. Therefore, the constipation symptoms Can be mitigated. The starch may specifically be isomaltooligosaccharide, fructooligosaccharide, galactooligosaccharide, xylooligosaccharide, maltooligosaccharide or mixed oligosaccharide. Double isomaltooligosaccharides may be more effective for the intestinal function activation than the other oligosaccharides. The starch may be contained in an amount of 2 to 10 parts by weight, more specifically 4 to 6 parts by weight, based on 100 parts by weight of the total composition of the abstinence-assisting composition.

In addition, the composition of the present invention may further comprise an energy source material. The energy source material can be any material that can supplement the insufficient body energy that may be generated by not eating food. Specifically, the energy source material may be a plant fermentation liquid. The substance is mildly acidic and may aid in the composition of beneficial bacteria in the intestines by improving the pH during dietary restriction.

The above-mentioned plant fermentation broth can be used as a fermented liquid such as dandelion, mulberry leaf, lobule, wormwood, motherwort, dandelion, pine needles, ganoderma, buttercups, And may be any one or more of the fermented liquids selected from the group consisting of vine, coconut, sole, shade, white dandelion, lobster, horse, shoemaker, shoemaker, mulberry, ginger tree, have. The plant fermentation broth may be fermented by a method known in the art, or commercially available ones may be used.

In a specific example of the present invention, the inventors of the present invention found that when the subjects were fasted for 10 days while consuming the abstinence-enhancing composition, the obesity-related indicators such as body weight and body fat amount were significantly decreased (see Table 5) Triglyceride, blood pressure, waist circumference, and fasting blood glucose were significantly decreased (see Tables 5, 6 and 8). In addition, the inflammation-related test showed a significant decrease in the high reactive C-reactive protein (hs-CRP) and C-reactive protein (CRP) (see Table 9) (Table 7, 8), and at the same time, the average value of all the indicators obtained from the biochemical tests including liver function, renal function and electrolyte index, etc. were within the normal range To confirm the stability of the abstinence-aid composition (see Table 8). On the other hand, as a result of the body fatigue and discomfort symptom survey conducted before and after the fasting period, the fatigue after the fasting period was significantly reduced even though the meal was restricted (see Table 11) The symptoms of discomfort such as headache, dizziness or vomiting were not different before and after the fasting period, and it was confirmed that when fasting while consuming the abstinence-aid composition of the present invention, fasting phenomenon after fasting was remarkably reduced, see Table 11 and 12 ).

Therefore, the composition of the present invention can be usefully used as an auxiliary composition for fasting therapy.

In addition, the present invention relates to a method for producing a ginseng extract, comprising: 1) obtaining a mixed extract of dermis, sine, ginger and ginseng, ginseng, omija and ginseng; And 2) a step of mixing and mixing the mixture extract of step 1), plum, maple sap, and morning syrup. Each material used in the above production method may have the above-mentioned characteristics.

According to the present invention, the step 2) may further include an energy source material. More specifically, the energy source material may further include a plant fermentation liquid.

In the above method, the mixed extract of step 1) may be extracted with water, a C ₁ to C ₂ lower alcohol or a mixture thereof as a solvent. The extraction method may be cold extraction, heat extraction, ultrasonic extraction or reflux cooling extraction. At this time, the extraction time may be 10 to 48 hours and 15 to 30 hours. According to one embodiment of the present invention, the extraction may be performed for 24 hours. The extraction may be repeated three to five times, specifically three times.

Further, in the above method, the step 2) may be performed at room temperature. According to one embodiment of the present invention, the temperature may be maintained at room temperature for 12 hours.

In a specific embodiment of the present invention, we added dermis, sine, ginger, mushroom root, omija and ginseng to the extractor after washing with purified water and then extracted at 90 ° C for 24 hours. Next, the extract was sterilized and filtered, and then the above filtrate was mixed with a maple sap and rice oligosaccharide in a mixture of a fermentation broth fermentation broth and a fermentation broth extracted from a field fungus. The mixture was allowed to stand at room temperature for 12 hours, An adjuvant was prepared.

The present invention also provides dietary supplements containing plum, dermis, sine, ginger, ginseng, omija, ginseng, maple sap, and orchard which have the above-mentioned characteristics.

Hereinafter, the present invention will be described in detail by the following examples. However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited thereto.

< Example  One > Manufacture of abstinence aids

&Lt; 1-1 > Production of Oriental herbal extract

First, a mixed extract of the medicaments contained in the abstinence aid was prepared. Specifically, 4 kg of dermis, 2 kg of sine, 2 kg of ginger, 6 kg of cormorant root, 3 kg of omija and 2 kg of ginseng were washed, based on the preparation of 180 kg of abstinence aid (Korea). The washed medicinal material was introduced into an extractor, and 76 L of purified water was added thereto, followed by extraction at 90 DEG C for 24 hours. The extracted extract was sterilized at 100 DEG C for 10 minutes, and then heat-treated using steam. The sterilized herbal extract was filtered through a 100-mesh filter having a pore size of 254 μm, concentrated to 1/4, and stored in a container.

<1-2> Preparation of abstinence aid

Using the herbal extract prepared in Example <1-1>, the abstinence aid was prepared as follows. Specifically, the mixture was weighed and mixed at a temperature of 100 占 폚 for 10 minutes and then subjected to a cooling treatment. The air was injected by opening the airout valve so that the remaining ethanol could be removed when the temperature reached 80 DEG C during sterilization. When the temperature of the filtrate after cooling is lowered to 40 캜 or less, the LB Maple Treat corp. Canada and rice oligosaccharide (Chung Jung Won Co., Korea) prepared in <1-1> Respectively. The mixture was allowed to stand at room temperature for 12 hours, and then filtered with a filter having a pore size of 5 mu m to prepare a shortening aid. The content of each component contained in the abstinence aid prepared by the above method is shown in Table 1, and the nutritional component is shown in Table 2.

material ratio(%) Maple sap 25 Fermented liquid of sulfur plum 10 Rice oligosaccharides (Chochung) 5 McMundong 3.3 dermis 2.2 Schisandra 1.7 sign 1.1 ginger 1.1 Ginseng 1.1 Extract of fermented soybeans 49.5 Total weight 100 100

nutrient Supplied amount Calories (kcal) 70.1 Carbohydrate (g) 17.36 (G) 14.57 Protein (g) 0.07 Total fat (g) 0.04 Saturated fat (g) 0 Trans fats (g) 0 Cholesterol (mg) 0 Salinity (mg) 0.61 Per 30 ml of abstinence aid

< Experiment Yes  One > Efficacy of fasting supplements

<1-1> Subjects

From December 2015 to February 2016, overweight and obese Koreans with body mass index (BMI) of 23 kg / m ^{2 and} over who visited three local clinics for obesity treatment were randomly assigned to the above- .

Pregnancy and lactation, anemia, hypotension, gallstone disease, tuberculosis, gastroduodenal ulcer and anorexia patients, insulin-dependent diabetes mellitus patients, untreated and untreated patients who are under 18 years of age, Patients with renal impairment (serum creatinine> 2.0 mg / dl), aspartate aminotransferase (AST) and alanine aminotransferase (ALT)> patients with hypertension (systolic blood pressure> 160 mmHg or diastolic blood pressure> 100 mmHg) 2.5 times the upper limit) were excluded from fasting therapy. Patients who had undergone other obesity treatment within the last 6 months were excluded from the study. As a result, the subjects were 33 persons in total, and the age distribution and the obesity degree of the subjects were shown in Tables 3 and 4, respectively. The age distribution of the subjects was 11 (33.3%) in their 20s, 6 (18.2%) in their 30s, 11 (33.3%) in their forties and 5 (15.2%) in their 50s. 16 patients (48.5%) and 17 patients (51.5%) were male (Table 3). Further, if classified by the body mass index of the subjects in BMI overweight (BMI of 23 to 24.9 kg / m ^2), seven (21.2%), obesity (BMI is 25 to 29.9 kg / m ²⁾ in 18 patients (54.5% ) And elevated obesity (BMI, ≥30 kg / m ² ) were in 8 patients (24.2%) (Table 4).

age

gender
total(%) female(%) male(%) 20-29 7 (21.2) 4 (12.1) 11 (33.3) 30 to 39 2 (6.1) 4 (12.1) 6 (18.2) 40 to 49 3 (9.1) 8 (24.2) 11 (33.3) 50-59 4 (12.1) 1 (3.0) 5 (15.2) total 16 (48.5) 17 (51.5) 33 (100)

BMI (kg / m ² )
gender
total(%)
female(%) male(%) Overweight (23 to 24.9) 7 (21.2%) (0.0%) 7 (21.2%) Obesity (25-29.9) 8 (24.2%) 10 (30.3%) 18 (54.5%) Altitude Obesity (≥30) 1 (3.0%) 7 (21.2%) 8 (24.2%) total 16 (48.5%) 17 (51.5%) 33 (100%)

<1-2> Fasting

The fasting regimen was performed over two days of preparation, 10 days of fasting and 3 days of recovery, followed by a diet of 15 to 30 days. During the fasting period, 40 minutes of aerobic exercise per day was carried out to prevent loss of muscle and to maintain basal metabolic rate. To maintain appetite suppression and chewing function and to provide a cooling sensation inside the room, Of xylitol gum (5 kcal / g) were allowed.

First, during the preparation period (2 days), the amount of food was not reduced, but caffeine, meat, and alcohol were restricted, and overeating was avoided. During the diaper (10 days), daily 180 ml of the dietary supplement was drunk in a total of 6 doses (between morning, morning and lunch, between lunch and dinner, between evening and evening and between bed and breakfast) 10-fold diluted in water. The total calorie intake per day was 420.6 kcal and the water intake was maintained at least 2.5 ℓ. On the first day of restoration (3 days), 300 g of uncooked rice was used to prepare meals for about 1,200 kcal, and on the second day about 1,500 kcal for meals and about 1,800 kcal (About 2/3 of the normal amount of food). In addition, the first day of the recovery period was uncontaminated and then low-salt.

The baseline test (baseline test) was performed in addition to the appropriateness test for fasting therapy from day 7 to day -1. In order to determine the effect of fasting therapy, Car examinations were performed.

<1-3> Physical measurement and Body composition  inspection

Changes in body composition and body composition before and after ingestion of the dietary supplement were examined.

First, body composition was measured using VENUS 5.5 (RESOURCE MEDICAL, Korea). Body weight, waist circumference, BMI, body fat mass, muscle mass, fat mass, body fat percentage, waist hip ratio, basal metabolic rate and visceral fat level were measured and the body weight, waist circumference and body composition The index items were analyzed by paired t-test.

As a result, all physical parameters and basal metabolic rate such as body weight, waist circumference, BMI, and body fat amount were decreased as shown in Table 5 below. On the other hand, visceral fat levels were decreased but not statistically significant (Table 5).

variable Before fasting After fasting change p-value Weight (kg) 79.21 + - 14.25 73.75 ± 13.43 -5.45 ± 1.13 *** <0.001 Waist circumference (cm) 89.75 +/- 10.64 82.46 ± 10.57 -7.28 ± 4.81 *** <0.001 BMI (kg / m ² ) 27.9 ± 3.44 26.01 ± 3.29 -1.89 ± 0.41 *** <0.001 Fat mass (kg) 25.23 + - 5.4 22.97 + 5.07 -2.26 ± 0.91 *** <0.001 Muscle weight (kg) 49.48 ± 9.89 46.58 ± 9.25 -2.89 ± 1.26 *** <0.001 Fat Weight (kg) 53.97 + - 10.67 50.78 + - 9.98 -3.19 + 1.30 *** <0.001 Body fat percentage (%) 31.98 + 4.17 31.24 + 4.09 -0.75 +/- 1.02 *** <0.001 Waist hip ratio 0.91 + 0.08 0.9 ± 0.08 -0.01 + -0.01 **   0.003 Visceral fat level 12.52 + - 2.84 11.61 ± 3.33 -0.91 + - 2.59   0.052 Basal metabolic rate (kcal) 1,388.55 ± 220.51 1,346.55 ± 207.33 -42 ± 21.12 *** <0.001 **, *** P <0.01 and P <0.001, respectively.

<1-4> Blood Pressure Test

Blood pressure was measured by using FT-500R PLUS (RESOURCE MEDICAL, Republic of Korea) after stabilizing the subjects for 5 minutes or more before and after the incubation period.

As a result, systolic blood pressure and diastolic blood pressure were significantly decreased as shown in Table 6 (Table 6). On the other hand, according to the results of the previous studies in which fasting was performed for only 5 days of water and saline, there was no significant change in systolic blood pressure after fasting, but only about 1.3 mmHg in mean diastolic blood pressure (Sung-Il et al., A Study on the Effect of Fasting on Human Body, Journal of Korean Academy of Family Medicine, Vol. 15, No. 12, 1995). However, in the present study, it was confirmed that blood pressure could be significantly decreased by taking the abstinence supplement during the 10 day fasting period.

variable Before fasting After fasting change p-value Systolic blood pressure (mmHg) 124.36 + - 16.36 113.52 ± 14.34 -10.85 +/- 14.61 *** <0.001 Diastolic blood pressure (mmHg) 76.85 ± 10 73.33 + - 9.6 -3.52 8.03 *   0.017 *, *** P <0.05 and P <0.001, respectively.

<1-5> Blood test

First, the whole blood and white blood cell counts of pre- and postmenopausal women were analyzed by using BC5800 (MINDRAY, Korea) to determine leukocyte, erythrocyte, hemoglobin (hemoglobin), erythrocyte volume, platelet count and refined leukocyte fraction (basophil, eosinophil, Neutrophils, lymphocytes, monocytes) were measured.

On the other hand, the blood biochemical test was performed by using AU680 (Beckman Coulter, USA) after subjects were allowed to fast for 8 hours or more. Specifically, the fasting blood glucose, blood urea nitrogen (BUN), creatine, total cholesterol, total protein, albumin, globulin, total bilirubin, direct bilirubin, aspartate aminotransferase, alanine aminotransferase (ALP), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), triglycerides, Na ⁺ , K ⁺ and Ca ²⁺ Were measured.

In addition, AU680 (Beckman Coulter, USA) was used to investigate the inflammatory response of subjects before and after the fasting period. Highly reactive C-reactive protein (hs-CRP), C-reactive protein (CRP), erythrocyte sedimentation rate (erythrocyte sedimentation rate, ESR).

As a result, as shown in Table 7, red blood cells, hemoglobin (hemoglobin) and erythrocyte volume were significantly increased as a result of whole blood cell and white blood cell fraction test. However, leukocytes and eosinophils were significantly decreased. On the other hand, the mean values of all indicators obtained from the whole blood and white blood cell counts remained within the normal range (Table 7). Previous studies of fasting for 5 days, taking only water and saline, showed an increase in hemoglobin (about 1 g / dl) after fasting but a decrease in the volume of red blood cells (about 1 g / dl) (Cho, Il et al., A Study on the Effects of Fasting on Human Body, Journal of Family Medicine, Vol. 15, No. 12, 1995). However, in this study, it was confirmed that even though we had a fasting period for 10 days, by supplementing the fasting supplement, the values of hemoglobin and hematocrit were significantly increased to improve the oxygen supply ability.

variable Normal range Before fasting After fasting change p-value Red blood cells (× 10 ⁶ / μl) 4 to 5.2 4.84 0.48 5.02 + - 0.42 0.18 ± 0.24 *** <0.001 Hemoglobin (g / dl) 13-16.5 14.50 ± 1.41 15.07 ± 1.16 0.57 + - 0.64 *** <0.001 Red blood cell volume (%) 36-47 44.17 + - 4.23 45.81 ± 3.59 1.64 ± 2.23 *** <0.001 Platelets (× 10 ³ / μl) 150-400 251.15 + - 43.41 251.24 + - 41.23 0.09 ± 26.42   0.984 White blood cells (× 10 ³ / μl) 4.5-10 7.18 ± 1.48 6.03 ± 1.9 -1.15 ± 1.58 *** <0.001 Enucleation (%) 0-3 0.56 ± 0.26 0.55 0.25 -0.01 + -0.33   0.916 Eosinophil (%) 0-5 2.93 ± 1.58 2.31 ± 1.26 -0.62 占 1.38 *   0.014 Neutrophil (%) 47-79.5 55.71 + - 6.53 54.67 ± 9.64 -1.04 ± 10.02   0.555 Lymphocyte (%) 15 to 45 33.73 + - 6.34 34.80 + - 8.47 1.07 + - 8.71   0.487 Monocyte (%) 2 to 10 7.06 ± 1.68 7.67 ± 1.62 0.60 ± 1.95   0.084 *, *** P <0.05 and P <0.001, respectively.

On the other hand, as shown in Table 8, blood biochemical test results showed that the values of total protein, albumin and globulin corresponding to the nutritional index after fasting were significantly increased. However, ALT decreased after fasting but was not significant. γ-GTP and blood urea nitrogen (BUN) were significantly decreased, and total bilirubin, direct bilirubin and creatine increased significantly. On the other hand, BUN / creatinine ratio (B / C) and fasting blood glucose were significantly decreased after fasting and total cholesterol was also decreased but not significant. In addition, high density lipoprotein cholesterol (HDL-C) and triglyceride were significantly decreased and low density lipoprotein cholesterol (LDL-C) was significantly increased. In addition, Na ⁺ and K ⁺ , which are electrolyte indicators, did not show any significant change after fasting, but Ca ^{2 +} was significantly increased. After the blastocyst, the mean values of all indicators obtained from the biochemical tests remained within the normal range (Table 8). On the other hand, in existing studies in which only water and saline were consumed and fasting was carried out for 5 days, total protein and albumin were not significantly different but were reported to decrease (Jung et al. , Journal of Family Medicine, Vol. 15, No. 12, 1995). However, in the present study, it was confirmed that even though we had a 10-day fasting period, the above-mentioned values were significantly increased by taking the fasting supplement, thus maintaining the body nutrition during the fasting period. In addition, in the above-mentioned literature, it has been reported that total cholesterol, triglyceride and ALT are significantly increased and γ-GTP is not changed by fasting. However, in this study, Triglyceride, triglyceride, ALT, and r-GTP tended to decrease or decrease significantly, thereby improving participants' health.

variable Normal range Before fasting After fasting change p-value Total protein (g / dl) 6-8.5 7.29 ± 0.36 7.79 ± 0.37 0.49 ± 0.45 *** <0.001 Albumin (g / dl) 3.5-5 4.58 ± 0.2 4.88 ± 0.25 0.3 ± 0.23 *** <0.001 Globulin (g / dl) 1.5 to 3.5 2.72 0.32 2.91 ± 0.33 0.19 ± 0.37 **   0.005 AST (IU / L) 0 to 40 24.88 + - 8.41 28.91 + - 8.48 4.03 ± 7.4 **   0.004 ALT (IU / L) 0 to 40 37.94 + 23.69 35.94 ± 19.71 -2.0013.27   0.393 γ-GTP (IU / L) 11 to 63 40.52 ± 30.53 29.88 ± 18.93 -10.64 +/- 12.82 *** <0.001 ALP (IU / L) 30 to 115 70.73 ± 15.31 74.36 ± 15.66 3.64 ± 12.23   0.097 Total bilirubin (mg / dl) 0.2 to 1.5 0.48 0.21 0.95 + 0.46 0.47 ± 0.39 *** <0.001 Direct bilirubin (mg / dl) 0.0 to 0.5 0.20 ± 0.06 0.38 + 0.13 0.17 ± 0.13 *** <0.001 BUN (mg / dl) 7-26 13.64 ± 3.29 8.98 ± 4.18 -4.66 + 4.97 *** <0.001 Creatine (mg / dl) 0.5 to 1.5 0.91 + 0.18 1.07 ± 0.18 0.16 + - 0.10 *** <0.001 BUN / creatinine ratio 7-29 14.88 ± 3.86 8.03 ± 3.92 -6.85 ± 5.21 *** <0.001 Fasting blood glucose (mg / dl) 70-110 99.21 + - 11.18 92.52 ± 10.9 -6.70 ± 13.19 **   0.006 Total cholesterol (mg / dl) 130-230 210.58 ± 40.79 209.42 + - 43.56 -1.15 ± 29.11   0.822 High density lipoprotein
Cholesterol (mg / dl) 35-99 56.12 ± 14.91 46.61 + - 11.64 -9.52 +/- 8.55 *** <0.001 Low-density lipoprotein
Cholesterol (mg / dl) 45 to 150 124.61 + - 43.33 145.55 + - 43.55 20.94 ± 30.88 *** <0.001 Triglyceride (mg / dl) 30 to 200 150.7 ± 105.17 90.67 ± 65.24 -60.09 ± 65.22 *** <0.001 Na &lt; ⁺ & gt ^; (mg / dl) 137-150 138.52 ± 1.37 137.64 ± 2.29 -0.88 ± 2.64   0.065 K ^& lt ^{; +} & gt ^; (mg / dl) 3.6 to 5.0 4.26 ± 0.36 4.18 ± 0.39 -0.08 ± 0.34   0.164 Ca ^{2 +} (mg / dl) 7.5-12.1 9.17 + - 0.35 9.56 ± 0.46 0.39 ± 0.52 *** <0.001 **, *** P <0.01 and P <0.001, respectively.

In addition, as shown in Table 9, the highly reactive C-reactive protein (hs-CRP) and the C-reactive protein (CRP) decreased significantly after the fasting, but the erythrocyte sedimentation rate (ESR) (Table 9).

variable Normal range Before fasting After fasting change p-value hs-CRP (mg / L) &Lt; 1.0 0.33 + - 0.54 0.14 + 0.17 -0.19 + 0.44 * 0.016 CRP (mg / L) <5 1.63 ± 1.77 0.92 + - 0.65 -0.71 + - 1.72 * 0.023 Erythrocyte sedimentation rate (mm / h) 0-14 8.82 + - 4.25 9.97 + - 6.67 1.15 ± 5.5 0.237 * Significant differences were identified at P <0.05

<1-6> Urinalysis

The urine test was performed before and after the experiment. Urine was analyzed using a EuroMeter 720 (SDI, Korea), and urine was analyzed using glucose, sugar, ketone, protein, nitrite, bilirubin, urobilinogen, occult blood, white blood cells, Respectively. The results of each item were quantified as 0 for '-', 0.5 for '+', 1 for '+', 2 for '+++' and 3 for '++++' And the results were evaluated.

As a result, as shown in the following Table 10, after the fasting, the ketone and ourobilinogen were significantly increased (Table 10).

variable Normal range Before fasting After fasting change p-value Glucose (glucose) 0 0.36 ± 1.17 0.03 0.12 -0.33 ± 1.13 0.100 Ketone 0 0.32 ± 0.73 1.50 ± 1.08 1.18 ± 1.39 *** <0.001 protein 0 0.36 ± 0.99 0.27 ± 0.40 -0.09 ± 1.06   0.683 Nitrite 0 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00     1,000 Yo bilirubin 0 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00     1,000 Urobilinogen 0-1 0.00 ± 0.00 0.73 ± 0.88 0.73 ± 0.88 *** <0.001 Urine potential bleeding 0 0.23 + - 0.57 0.42 ± 0.86 0.20 ± 1.00   0.282 Leukocyte / HPF 0-4 2.03 ± 2.23 2.76 ± 3.86 0.73 + - 4.19   0.327 Urinary Blood Cells / HPF 0-1 1.67 ± 2.48 2.70 ± 3.90 1.03 + - 4.47   0.195 Urine pH 5.0 to 8.5 6.02 ± 0.63 5.82 ± 0.37 -0.20 ± 0.71   0.119 Urinal weight 1.005-1.030 1.022 0.005 1.021 ± 0.006 -0.001 ± 0.007   0.447 HPF: high power field
*** P <0.001 confirms significant difference

<1-7> Questionnaire Survey

The revised Chalder's fatigue scale and the rating scale for subjective symptoms were used to analyze the results.

Specifically, the fatigue questionnaire consisted of 7 items of physical fatigue, 4 items of mental fatigue, total 11 items, and each scale was divided into 4 stages (good = 1, similar to normal) = 2 points, less than usual = 3 points, and much less than usual = 4 points). In addition, the subjective symptom questionnaire was used to assess the eight types of discomfort symptoms (hunger, dizziness, heartburn, headache, loudness, somnolence, insomnia and skin rash) No = 1, light = 2, middle = 3, severe = 4 and very severe = 5).

As a result, fatigue after the fasting was significantly improved as shown in Tables 11 and 12 below. By item, you can take a break from the item "Do you need more rest?", "Are you having symptoms of sleepiness?", "Are you having difficulty or difficulty in starting a new job? (Table 11). In addition, there were no significant differences in the subjective symptoms between before and after the abstinence period in all of the eight items such as hunger, dizziness, heartburn, headache, loudness, somnolence, insomnia and skin rash (Table 12). In general, fasting with only drinking water increases the feeling of helplessness and fatigue after fasting, and it is difficult to perform singles with more than five days in daily life because of dizziness, headache and stomach symptoms. And the fasting can be carried out only under intensive management. However, as described above, the adverse effects due to fasting were alleviated by ingesting the abstinence aid, and it was confirmed that the abstinence of 10 days or more could be performed in parallel with daily life.

Question Before fasting After fasting change p-value Are you tired? 2.06 ± 0.61 1.79 + - 0.55 -0.27 ± 0.80 0.059 Need more relaxation? 2.15 ± 0.57 1.76 ± 0.50 -0.39 + 0.79 ** 0.007 Do you have symptoms of sleepiness? 2.15 + 0.44 1.85 0.71 -0.30 ± 0.81 * 0.039 Do you find it hard or difficult to start a new job? 2.06 ± 0.43 1.79 + - 0.55 -0.27 ± 0.63 * 0.018 Are you feeling sick? 2.15 ± 0.51 2.15 + - 0.62 0.00 ± 0.87 One Do you feel weak muscle strength? 2.21 0.55 2.24 ± 0.71 0.03 0.81 0.831 Do you feel weak overall? 2.21 ± 0.65 2.12 ± 0.65 -0.09 ± 1.07 0.629 Focusing on work and thinking.
Are you tired? 2.09 ± 0.46 1.91 ± 0.58 -0.18 ± 0.68 0.136 When you think about it, do you feel dizzy and unhappy? 2.09 ± 0.52 1.85 ± 0.67 -0.24 ± 0.79 0.088 I want to say something, but can not talk properly? 2.09 ± 0.58 1.85 ± 0.51 -0.24 ± 0.75 0.073 Do you have low memory? 2.16 ± 0.57 1.79 + - 0.55 -0.30 ± 0.88 * 0.012 total 23.36 + - 4.36 21.09 + - 4.54 -2.27 ± 6.29 * 0.046 *, ** P <0.05 and P <0.01 were found to be significantly different

Subjective symptoms Before fasting After fasting change p-value Hunger feeling 1.85 0.80 1.94 + 0.79 0.09 ± 1.13 0.647 whirl 1.42 ± 0.79 1.36 ± 0.82 -0.06 ± 1.06 0.744 water brash 1.33 + - 0.82 1.73 ± 0.91 0.39 ± 1.12 0.051 headache 1.39 ± 0.79 1.24 ± 0.56 -0.15 + 0.71 0.231 Languor 1.24 0.71 1.33 + 0.78 0.09 ± 1.01 0.609 It's a bitter 1.52 + - 0.97 1.42 ± 0.87 -0.09 ± 0.98 0.598 Blow 1.48 ± 0.83 1.36 ± 0.86 -0.12 ± 0.96 0.474 Skin rash 1.24 ± 0.79 1.15 ± 0.62 -0.09 ± 0.84 0.54 *, ** P <0.05 and P <0.01 were found to be significantly different

Claims (20)

Plum, dermis, sine, ginger (ginseng), mukmundong, omija, ginseng, maple sap, and orchard.
The abstinence-reduction composition according to claim 1, wherein the plum is at least one selected from the group consisting of oatsu-yaki, pickling-room, and yellowish room.
2. The composition according to claim 1, wherein the plum is a plum fermentation broth.
4. The composition according to claim 3, wherein the plum is contained in an amount of 5 to 20 parts by weight based on 100 parts by weight of the total composition.
[2] The composition according to claim 1, wherein the dermis is contained in an amount of 1 to 5 parts by weight based on 100 parts by weight of the total composition.
The cutting aid composition according to claim 1, wherein the sine is contained in an amount of 0.5 to 3 parts by weight based on 100 parts by weight of the total composition of the cutting aid composition.
2. The composition according to claim 1, wherein the ginger is at least one selected from the group consisting of ginger, ginger, excess, and turmeric.
[2] The composition according to claim 1, wherein the ginger is included in an amount of 0.5 to 3 parts by weight based on 100 parts by weight of the whole composition.
The abstinence-reduction composition according to claim 1, wherein the gutters are included in an amount of 2 to 10 parts by weight based on 100 parts by weight of the total composition.
The abstinence-reduction composition according to claim 1, wherein said omisza is contained in an amount of 0.5 to 5 parts by weight based on 100 parts by weight of the total of the abstraction-aid composition.
[2] The composition according to claim 1, wherein the ginseng is contained in an amount of 0.5 to 3 parts by weight based on 100 parts by weight of the total composition.
The abstinence-reduction composition according to claim 1, wherein the maple sap is contained in an amount of 15 to 40 parts by weight based on 100 parts by weight of the abstraction-aid composition.
The abstinence-reduction composition according to claim 1, wherein the starch is contained in an amount of 2 to 10 parts by weight based on 100 parts by weight of the total composition of the abstraction-providing composition.
The abstinence aid composition of claim 1, further comprising an energy source material.
15. The composition according to claim 14, wherein the energy source material is a plant fermentation broth.
1) obtaining a mixed extract of dermis, sine, ginger and (ginseng), mukmundong, omija and ginseng ; And
2) a step of mixing and mixing the mixed extract of step 1), plum, maple sap, and morning syrup, and allowing to stand.
The method according to claim 16, further comprising an energy source material in step 2).
17. The method according to claim 16, wherein the mixed extract is extracted with water, a C ₁ to C ₂ lower alcohol or a mixture thereof as a solvent.
The method according to claim 16, wherein the step (2) is carried out at room temperature.
Dietary supplements including plum, dermis, cod, ginger (生薑 科), moonmoon dong, omija, ginseng, maple sap and mackerel.