CN105623995B

CN105623995B - Large-fruited Chinese hawthorn aperitif and beverage and preparation method thereof

Info

Publication number: CN105623995B
Application number: CN201610125815.1A
Authority: CN
Inventors: 胡伯虎; 胡伟; 胡勇; 胡馨木
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-03-06
Filing date: 2016-03-06
Publication date: 2018-11-09
Anticipated expiration: 2036-03-06
Also published as: CN105623995A

Abstract

The invention discloses a kind of plant liquor promoting digestion and beverages and preparation method thereof --- large-fruited Chinese hawthorn aperitif and beverage and preparation method thereof.Its plant and dispensing are：Hawthorn, coloured malt, dried orange peel, stir-baked SEMEN RAPHANI, fennel seeds, fingered citron, sea-buckthorn, Radix Glycyrrhizae, cassia bark, xylo-oligosaccharide, potassium sorbate, 38 degree of white wine or grape wine or water.The composition of the present invention has significantly increasing action gastric juice total amount, gastric juice discharge rate and pepsin discharge rate tool, there is apparent small intestine push speed increasing action, display tool to help digest and gastroenteritic power is promoted to act on the slow model of mouse gastrointestinal peristalsis.Have many advantages, such as it is safe and effective, it is without side-effects.Present invention preparation takes modern advanced ultrasound assisted extraction technique, so that effective ingredients in plant availability is significantly improved, energy conservation and environmental protection, microwave is used to radish seed and quantifies frying, suitable for industrial production, it is a kind of novel aperitif and beverage, China's aperitif will be expanded and lack situation.

Description

Large-fruited Chinese hawthorn aperitif and beverage and preparation method thereof

Technical field

The present invention relates to field of beverage, and in particular to a kind of plant liquor and beverage and preparation method thereof promoting digestion --- Large-fruited Chinese hawthorn aperitif and beverage and preparation method thereof.

Background technology

One word of aperitif derives from Latin aperare, and justice is " opening ", refer to before the meal, after drunk and can stimulate stomach Mouthful, open appetite drinks.Aperitif is born in Middle Ages, and people like tasting medicinal liquor before lunch or adding fragrance at that time Grape wine；Thinking certain plants always has effects that open appetite.Aperitif has become can not in social activity, family life Or scarce component part；If asking someone to have a meal without asking him to drink one glass of aperitif, this situation is incredible , aperitif inherently Yi Zhong spindle is asked.The kind of aperitif has tens kinds in west.External traditional aperitif product mainly divide For 3 types：Vermouth, Bitters, mattress cordiale.Wine degree is at 18 ° or so.Aperitif is also divided into aperitif and postprandial by west Wine：The wine that can be stimulated appetite can be known as aperitif.Aperitif characteristic is can not only to stimulate appetite, and also nourishes, is strong Strong, stomach invigorating and other effects, aperitif mainly have ligueur, fermented milk, GET PEPPERMINT to spend wine etc.；Digestivo is primarily referred to as drinking after the meal Can digestive drinks, Digestivo includes brandy, mescal, whiskey, ligueur, fermented milk, and GET PEPPERMINT spends wine Deng.Remy Martin, celestial No.1 etc. of floaing are Digestivo Typical Representatives.Celestial No.1 of floaing is actually that rum perfuming is formulated, He is such as Banana Wine, almond wine, dried orange peel wine, and coffee minstra is all more typical Digestivo.

Modern aperitif is allotment wine mostly, makees wine base with grape wine or strong drink, the immersion of vegetable raw material is added These raw materials are added in distillation in object.Aperitif mainly has effects that appetizing is planted using grape wine or Spirit as raw material addition Root, stem, leaf, medicinal material, fragrance of object etc. are formulated.Foreign countries add into anise, root of Dahurain angelica root, almond, lemon peel, coriandrum, Wormwood, peppermint, Dutch root, fennel, beautiful cassia bark etc..

The very good appetizing of many of China's traditional Chinese medicine effect promotes digestion autonomic drug (1) (2).It represents recipe such as：Member Famous doctor's ZHU Dan-xi (Christian era 1281-1358) in generation《Danxi's experiential therapy》Pachyma Compound Digestive Tonic Pill, by hawthorn (coke), Medicated Leaven (stir-fry), The tuber of pinellia (toast), radish seed, Poria cocos, dried orange peel, Fructus Forsythiae composition.It can help digestion, lead stagnant and stomach.It is stagnated for dyspepsia, abdominal fullness and distention, Rotten acid regurgitation, do not feel like eating, curative effect is rather good, the dashanzha bolus for selecting its main ingredient to develop later, by hawthorn, Divine Comedy, three taste medicine group of malt At.With help digestion, except product, digestant function.The common feature of the two good recipes is to remove Medicated Leaven, outside two taste of the tuber of pinellia, is all Also the common plant that medicine is also eaten, it is resourceful.We are according to this feature, on the basis of inheriting forefathers' invaluable experience, tie and existing For achievement in research, the tuber of pinellia, Poria cocos, Fructus Forsythiae are subtracted.Constitute by hawthorn, coloured malt, stir-baked SEMEN RAPHANI, dried orange peel, fennel seeds, fingered citron, The appetizing of the primary raw materials such as sea-buckthorn, Radix Glycyrrhizae, cassia bark promotes the new formula of digestion, is used for crowd indigestion person.Due to the party with Hawthorn is monarch, and coloured malt, dried orange peel is minister, and stir-baked SEMEN RAPHANI is assistant, and fennel seeds, fingered citron, sea-buckthorn, Radix Glycyrrhizae, cassia bark are to make, major function It is aid digestion for " appetizing ", therefore named life large-fruited Chinese hawthorn aperitif and beverage.Due to the difference of Chinese and Western culture, Chinese tradition spirits culture is focused on The color of wine, although medicinal liquor is a lot of, based on tonic, so far in the market there is not yet special aperitif, this wine can Can be the first wine of appetizing of China.

In order to determine the creativeness of the present invention, we are careful, comprehensively retrieve the home position of patent family：Wu Xiujie, A kind of health care tea liquor and preparation method thereof for promoting to digest and assimilate.Application publication number：The weight of its constitutive material of CN104327996A Measuring part is：Tribute chrysanthemum 40-50, dietary fiber 20-30, stir-baked SEMEN RAPHANI 4-6, melanoidin malt 25-30, Poria cocos 3-5, orange peel 2-4, celery residue 4-6, guar gum 3-4, spiked gingerlily fruit 1-2, broadbean stem 2-4, stir-baked MASSA FERMENTATA MEDICI-NALIS 3-5, radix ephedrae 1-2, wheat thorn rattan 1-2, emblic extraction Liquid 0.4-0.5, smoked plum extractive 1-2, maltase 0.4-0.6, lactic acid bacteria 2-3, brewer's yeast 1-2, composite nutrient 3-4, wheat Bud sugar 6-8；This health care tea liquor has taste flavor, full of nutrition, adds the Chinese herbal medicine of a variety of integration of drinking and medicinal herbs, stomach is contributed to disappear Change, improve function of intestinal canal, reduce gastrointestinal stimulation, while being wherein rich in the nutriments such as cellulosic, maltose, it is anti-that human body can be increased Oxidizing force improves body and skin health, and to loss of appetite, person has certain curative effect.But the party is complicated, and radix ephedrae etc. is by defending Life portion regulation cannot enter drink.Hunan University of Traditional Chinese Medicine, which is thanked, avenges a kind of handsome, health care tea bag of spleen benefiting and stimulating the appetite.Application publication number： CN104351436A.Material medicine：5-10 parts of bitter citrus immature flower, 5-10 parts of fructus amomi, 10-20 parts of sea-buckthorn, 10-20 parts of hawthorn, coloured malt 10-20 parts, 20-25 parts of the membrane of a chicken's gizzard, 10-20 parts of radish seed, above-mentioned raw materials drug is cleaned to be placed in 60-70 DEG C of baking oven and is dried It is dry；Drug after drying is crushed, 80 mesh sieve is crossed；Drug powder after sieving is packaged in tea bag packet.The health care of the present invention Tea bag plays spleen benefiting and stimulating the appetite during usually drinking, and eliminates various eating accumulations, the effect that treatment children have no appetite； The health care tea bag of the spleen benefiting and stimulating the appetite of the present invention is tea bag packet, easy to carry, clean hygiene.But fructus amomi has pungent taste, the membrane of a chicken's gizzard variable Matter is unfit to do drink, and suitable technique falls behind.Applicant：A kind of digestion-promoting stomachic of Liu Shao Nas and preparation method thereof application publication number： CN104288615A is formed：10-16 parts of ginseng, 10-20 parts of hawthorn, 8-12 parts of Radix Glycyrrhizae, 8-10 parts of the membrane of a chicken's gizzard, six 12-18 parts of songs, 8-12 parts of fig, 10-16 parts of radish seed, 10-20 parts of malt, 10-16 parts of xylitol, 10-14 parts of honey, 30-50 parts of starch, 50-70 parts of water；The digestion-promoting stomachic of the present invention, preparation method is simple, and product recovery rate is high, can reduce production cost, can disappear Productization is stagnant, spleen benefiting and stimulating the appetite and stomach are soothing the liver, belongs to healthy medicine trade, has vast potential for future development.But the product with drug application very Difficulty is by evaluation, because similar product is a lot of.Inventor：A kind of stomach strengthening and digestion promoting tea granules of Zhang Huawei and preparation method thereof Shen Qing Publication Number：CN104172164.Raw material is made：Poria cocos 4-5, sealwort 3-4, the membrane of a chicken's gizzard 5-6, radish seed 3-4, dried orange peel 4.5-5, hair root 1-2, achickenclaw ginseng 2-3, maltodextrin 10-15, hawthorn 20-22, pawpaw 10-12, coix seed 6-7, milk powder 3-4, soda water 8-9, White sugar 4-5, mung bean 5-6, wild cabbage 7-8, rye flour 15-20, abalone kernel 4-5, shrimp med 10-11, caraway 6-7, vinegar 45- 50, lactic acid bacteria 0.1-0.12, nourishing additive agent 10-11.The Chinese medicine added in the tea granule of the present invention can kill after vinegar steams It goes out the harmful substances such as the germ being attached in medicinal material, pathogenic microorganisms and worm's ovum, also pharmacological property can be made milder, in chicken therein The collocation hawthorn such as gold, dried orange peel can make the present invention have the function of stomach strengthening and digestion promoting, in addition, each raw material proportioning science in the present invention, Unique flavor.But sealwort in being formulated, achickenclaw ginseng cannot enter drink by Ministry of Public Health's regulation.Inventor：Xie Xuejiao has strengthening spleen and nourishing stomach Chinese medicine composition of effect and its preparation method and application application publication number：CN104127758A.The Chinese medicine composition has Effect ingredient is mainly prepared by the raw material of following weight parts：2-40 parts of dendrobium candidum, 1-40 parts of Chinese yam, 2-40 parts of sea-buckthorn, mountain 2-40 parts of short, bristly hair or beard, 2-40 parts of coloured malt, 2-40 parts of the membrane of a chicken's gizzard, 2-40 parts of radish seed.Chinese medicine composition of the present invention has notable Strengthening spleen and nourishing stomach effect, taste patient loss of appetite, indigestion and the bored swollen symptom of chest and abdomen can be significantly improved.But in chicken Golden mutability shield.Inventor：Hu Anran, a kind of stomach nourishing tea.Application publication number：CN104095100A.Selected hawthorn, dried orange peel, malt, The a variety of integration of drinking and medicinal herbs of pawpaw, fig, Radix Glycyrrhizae, matrimony vine, dark plum, nutmeg, mulberries, radish seed, almond, cape jasmine, fingered citron, cloves Food materials are crushed with stainless steel pulverizer 40-80 mesh and are packed into the techniques such as heat-sealing type tea filter paper bag alternative tea is made, have collection dietotherapy, Prevent, conditioning triple function is in the unique advantage of one.But cape jasmine, cloves tea making are bitter and pungent.Inventor：Community in Baiyunshan, Guangzhou star Group (medicine company) limited liability company Jiang Li is beautiful；The preparation method of valiant fighter's digestion promoting oral liquid.Application publication number：CN104083617A. The invention discloses a kind of preparation methods of valiant fighter's digestion promoting oral liquid, include the following steps：Take Radix Astragali, rhizoma atractylodis macrocephalae, dried orange peel, wheat Winter, radix scutellariae, stir-baked FRUCTUS CRATAEGI and stir-baked SEMEN RAPHANI, add water to cook, and obtain extracting solution, it is filtered through supermicro filtration membrane, obtain clear liquid, then by clear liquid It is concentrated into clear cream, in clear cream plus refined honey, potassium sorbate and purified water, mixing are filtered through tubular ultra-filtration membrane equipment, you can.This It invents the preparation method and is determined that the best approach and optimum condition for preparing valiant fighter's digestion promoting oral liquid, this method first use ultra micro Filter membrane effectively filters extracting solution, without the refrigeration process in traditional mode of production, then passes through tubular ultra-filtration membrane filtering technique pair Liquid after preparation carries out end-filtration, significantly improves the content of scutelloside in liquid clarity and liquid, improves production Product quality, it is ensured that the quality stability of product can also be realized and be produced without alcoholization, significantly shorten the production cycle, reduce Energy consumption and production cost are suitable for producing on a large scale.But invention is drug, on-beverage.

Though the above patent application has their own advantages, our composition is after selected, formula refining, and technique is advanced.

We queried from after 12 days December in 2003 from State Food and Drug Administration's data query through state The essential information of the health food of food and medicine Surveillance Authority of family examination & approval.The contents list of " national OTC " shares The contents list of 12209 records, wherein keyword " promoting digestion " shares 80 records, the content row of keyword " hawthorn " Table shares 10 records.Wherein as promote digestion similar product it is representational have it is following several：The Jiangxi good medicine company of profit has The good good shellfish shellfish oral solution of board in east that limit company produces, functional component/significant component content contain per 100ml：General flavone 65.4mg, Primary raw material hawthorn, malt, radish seed, Poria cocos, Chinese yam, xanthans, stevioside, water；Correct the limited public affairs of bioengineering in Jilin Amendment board this only promise oral solution produced is taken charge of, functional component/significant component content contains per 100ml：Isomalto-oligosaccharide 15g, Zinc 10mg, iron 30mg, primary raw material isomalto-oligosaccharide, the membrane of a chicken's gizzard, coloured malt, hawthorn, stir-baked SEMEN RAPHANI, zinc gluconate, second Ethylenediamine tetraacetic acid (EDTA) ferrisodium, sucrose, deionized water, flavoring banana essence；Jiangxi Jiangzhong Pharmaceutical (Group) Co., Ltd., ProductName Claim：Board eats power beverage in river, and ingredient/significant component content contains per 100ml：General flavone (in terms of rutin) 2.17mg, it is main former Expect hawthorn, malt, Chinese yam, dried orange peel, honey element, sucrose, citric acid, potassium sorbate, strawberry essence, distilled water, eating method and Amount 3 times a day, each 50ml；It is shaken up when eating after meal, is edible.50ml/ bottles, 150ml/ bottles, 300ml/ bottles of product specification； The promoting digestion and removing indigestion oral solution of Nanchang Chuan Qi health products Co., Ltd, primary raw material hawthorn, the membrane of a chicken's gizzard, Semen Lablab Album, Poria cocos, glucose Sour zinc, thiamine hydrochloride, taurine, sucrose, deionized water；The huge day board stomach invigorating that Jiangxi Ju Bioisystech Co., Ltd produces disappears Change oral solution, functional component/significant component content contains per 100ml：General flavone (in terms of rutin) 101mg；The primary raw material Beishan Mountain Short, bristly hair or beard, malt, Rhizoma Atractylodis Macrocephalae, Poria cocos, Semen Lablab Album, the membrane of a chicken's gizzard, radish seed, sucrose.The above product suitable population is indigestion person, no Suitable population without.

Our contents from State Food and Drug Administration's data query national OTC keyword " large-fruited Chinese hawthorn " List shares 0 record, and the contents list of domestic drug " keyword " " large-fruited Chinese hawthorn " shares 0 record, shows this product name None with existing drug and health products is claimed to repeat, formula composition is also with ancient prescription and existing drug and health food entirely without repetition With it is identical.

Invention content

The purpose of the present invention：One is to provide more effectively whet the appetite a kind of digestant plant liquor and beverage；Two are to provide this The preparation method of wine and beverage.

1. formula and dosage

The formula and dosage of the present invention is the determination by repeated screening according to pertinent literature and application practice, each group Good effects could be obtained in following weight range by closing object dosage：

20-70 parts of hawthorn, 7-20 parts of coloured malt, dried orange peel 5-15, stir-baked SEMEN RAPHANI 10-30, fennel seeds 5-10, fingered citron 5-10, Sea-buckthorn 10-20, Radix Glycyrrhizae 6-12, cassia bark 5-10,2-10 parts of xylo-oligosaccharide, 0.3-0.6 parts of potassium sorbate.

Preferably 50 parts of hawthorn, 15 parts of coloured malt, 10 parts of dried orange peel, 20 parts of stir-baked SEMEN RAPHANI, 8 parts of fennel seeds, 8 parts of fingered citron, sand 15 parts of spine, 8 parts of Radix Glycyrrhizae, 8 parts of cassia bark, 5 parts of xylo-oligosaccharide, 0.5 part of potassium sorbate.

Further, in formula of the invention further include 2-8 parts of Himalayan mayapple fruit, preferably 5 parts.

Further, in formula of the invention further include 2-8 parts of the stem of noble dendrobium, preferably 3 parts.

Dosage is less than this range, and then effect is poor, the light deficient pool of color, and dosage is more than this range and then easily contains sediment, and color is heavy muddy, Poor taste.

Hawthorn rosaceous plant large-fruited Chinese hawthorn Crataegus pinnatifida Bge.var.major N.E.Br. or mountain The dry mature fruit of short, bristly hair or beard Crataeguspinnatifida Bge..Crataegolic acid is sweet, tepor.Enter spleen, stomach, Liver Channel.Function invigorating the spleen Stomach, it is aid digestion, be the top grade of meat stagnation of disappearing, be widely used in indigestion etc., therefore it is monarch to select, therefore its dosage is being formulated In account for large percentage；Research shows that：Hawthorn contains flavone of hawthorn fruit and a variety of organic acids, and contains lipolytic enzyme, after entering stomach, can enhance enzyme Effect promotes carnivorous digestion, cholesterol is contributed to convert.Hawthorn is full of nutrition, almost all nutritional ingredients containing fruit, special It is not that organic acid and ascorbic content are higher.Hawthorn sweet and sour taste is suitble to eat raw, and is suitble to be processed into health food.It is modern Medical research proves：Still there is hawthorn decompression, lipid-loweringing to increase the effect (3) (4) of coronary flow and arrhythmia etc..

Malt for the ripening fruits of grass family annual herb plant barley HordeumvuigareL. is dry through germination and At.Nature and flavor：Sweet and neutral.Channel tropism：Taste.Huan Compendiums of Materia Medica》It says：" can digest all rice, face, all pulp dyspepsias " is promoting digestion and removing indigestion Key medicine.Applied to dyspepsia, loss of appetite etc.；Ingredient mainly contains α-and beta amylase (amylase), catalyzing enzyme (catalyticase), peroxidating isomerase (peroxidisomerase) etc..Beta amylase can be by amylose complete hydrolysis at wheat Bud sugar, alpha amylase are then allowed to resolve into short straight key polyglucose (i.e. dextrin), and the latter can be that beta amylase is hydrolyzed into malt again Sugar.Therefore starch is decomposed into maltose and dextrin under the action of α and beta amylase.In addition to malt itself can promote digestion, wheat Bud decoction also has slight facilitation (5) to the secretion of hydrochloric acid in gastric juice and pepsin.

Dried orange peel is the drying and ripening fruit of rutaceae orange Citrus reticulata Blanco and its variety Skin.Dried orange peel acrid flavour is bitter, warm-natured, has effects that warming stomach for dispelling cold, regulating qi-flowing for strengthening spleen, is suitble to stomach turgor, indigestion, loss of appetite Etc. symptoms people it is edible.Modern research shows that containing the ingredients such as a large amount of volatile oil, hesperidine, vitamin B, C, its institute in dried orange peel The volatile oil contained has mild stimulation to gastrointestinal tract, can promote the secretion of digestive juice, and excludes pneumatosis in enteron aisle, increases appetite. Volatile oil contained by it has mild stimulation to gastrointestinal tract, can promote the secretion of digestive juice, and excludes pneumatosis in intestinal tube, increases food It is intended to.The bitter substance of dried orange peel is using limonin and bitter principle as representative " limonoid ", and this limonoid taste is gentle, It is easily soluble in water, contributes to the digestion of food.When dried orange peel is used for cooking dishes.Its bitter taste mutually reconciles with other tastes, can shape At original in style flavor.Dried orange peel plays dual regulation to pipe smooth muscle.Dried orange peel both contained excited stomach movement at Point --- aurantiamarin, have the function of reinforce WeiDongLi Capsule, and energy whetting appetite, qi-regulating de-swelling；Again containing relaxing smooth muscle at Point --- volatile oil, thus the spasmic pain of stomach can be released.It is the dyspeptic common medicine for the treatment of, does not relax especially, eats to stomach It is intended to the effect of depressed, bored swollen, pain, nausea, the general symptom to be spat are more suitable, and relieving the gastric disorder and regulating vital energy, de-swelling are alleviated pain, therefore is selected as Make medicine (6).

The SEMEN RAPHANI that radish seed is commonly called as.It is the dry mature seed of crucifer radish (Raphanus sativusL) Chemical analysis：Seed contains sinapine (sinapine) and fat oil 30%, contains a large amount of erucic acid (erucic acid) and Asia in oil Oleic acid (linoleic acid), leukotrienes (linolenic acid) also contain brassicasterol (brassicasterol) and 22- Dehydrogenation campesterol (22-dehydrocampesterol).It is separately plain (raphanin) containing radish.Radish seed nature and flavor, pungent, sweet and neutral. Return lung, spleen, stomach.《Compendium of Materia Medica》It records, stir-baked SEMEN RAPHANI has work(such as " lower gas Dingchuan control phlegm, help digestion and eliminate flatulence, sharp stool and urine " With.For controlling retention of food and drink, abdominal distention, constipation, therefore select as assistant；Experiment display：Radish seed difference extract is to reality The property tested Mouse Stomach defecation and Intestinal pushing remarkably promote effect, and radish seed oil, radish seed the water extracted immersing paste have mice with constipation logical Just it acts on, a large amount of clinical reports are stagnated with stir-baked SEMEN RAPHANI Therapeutic diet, and abdominal distention, constipation truly has good effect, and can make digestion not Good symptom is obviously improved, therefore thinks that radish seed " promoting the circulation of qi helps digestion " acts on, and is substantially that can promote gastrointestinal motility.In addition, still having disease-resistant The effect of pathogenic microorganism, detoxication and antihypertensive effect, therefore be selected into as assistant (7).

Fennel seeds acrid flavour, it is warm-natured.Return liver,kidney,spleen, stomach.Function eliminating cold to stop pain, regulating qi-flowing for harmonizing stomach are treatment indigestions Common food.

Fingered citron whole body is all precious.Root, stem, leaf, flower, fruit can be used as medicine, pungent, bitter, sweet, warm, nontoxic；Enter liver, spleen, stomach three Through there is a variety of medicinal functions such as regulating qi-flowing for eliminating phlegm, preventing or arresting vomiting dissipate-swelling, liver soothing and speen fortifying and stomach.It is swollen to the indigestion of common people, chest and abdomen It is bored, there is significantly more curative effect.Long term usage plays the role of health-care.Zoopery is shown：This product alcohol extract is in vitro to rat, rabbit Intestinal tube has obvious inhibiting effect, intravenous injection administration also to have same inhibiting effect in body intestinal tube to anesthetized cat, rabbit.To acetylcholine Caused rabbit duodenum spasm has notable spasmolysis.

Sea-buckthorn sea-buckthorn is economic plants, and it can be served for product, and pharmaceutically acceptable.Fruit and oil have very high medical value, energy Gastric duodenal ulcer and indigestion etc. are treated, the illnesss such as chronic superficial gastritis, atrophic gastritis, colitis are treated Effect is notable；Cholesterol can be reduced, allevating angina pectoris breaking-out also prevents the effect of coronary atherosclerotic heart disease.

Radix glycyrrhizae taste is sweet, it is mild-natured and, enter the heart, spleen, lung, stomach four and pass through.It invigorates the spleen and benefits qi, clearing heat and detoxicating, expelling phlegm and arresting coughing, emergency Analgesic, coordinating the drug actions of a prescription.It is usually used in weakness of the spleen and the stomach, lassitude hypodynamia.Radix Glycyrrhizae has similar adrenocortical hormones fuction.Histamine is drawn The gastroxia risen has inhibiting effect；And there is antiacid and alleviation gastrointestinal smooth muscle spasm effect.Remove the leaching of glycyrrhizin Flavonoid glycosides have obvious protective function to Rat Experimental ulcer in cream and Radix Glycyrrhizae.In addition, Radix Glycyrrhizae is also good at mediation property of medicine, solution hundred The poison of medicine.

Cassia bark acrid-sweet flavor, property heat, entering kidney, spleen, bladder warp has complement sun, warming spleen and stomach, removing internal cold, coronary circulation-promoting pain-relieving and antidiarrheal The effect of stomachache with cool feeling and abdominal pain diarrhea for deficiency-cold in spleen and stomach,

Xylo-oligosaccharide also known as wood oligose are using refining corncob powder as raw material, by the production work such as enzyme process separation, purification A kind of functional sugar that skill is process is a kind of sweetener with special role.Xylo-oligosaccharide is by 2-7 xylose molecule The functional polymerization sugar being combined into β-Isosorbide-5-Nitrae glycosidic bond.With the soyabean oligosaccharides, oligofructose, oligomeric used in usual people Isomaltose etc., which is compared, has unique advantage, it can selectively promote the proliferation activity of intestinal bifidobacteria.Its bifid Because subfunction is 10-20 times of other polymerization carbohydrates.Xylo-oligosaccharide is to be proliferated the strongest product of Bifidobacterium function in polymerization carbohydrate One of kind, its effect of property is nearly 20 times of other polymerization carbohydrates, does not have to hydrolyze the enzyme of xylo-oligosaccharide in human gastrointestinal tract, so It is preferably that Bifidobacterium is utilized that it, which can be directly entered big enteral, promotes Bifidobacterium Bifidum proliferation while generating a variety of organic acids.Drop Low enteron aisle pH value inhibits harmful bacteria growth, so that probiotics is largely proliferated in enteron aisle, xylo-oligosaccharide has stronger absorption to pathogen Power, such as Escherichia coli, enteritis door Salmonella, Friedlander's bacillus, Aeromonas hydrophila can be adsorbed onto on xylo-oligosaccharide, by It is not degraded by the digestive ferment in enteron aisle in xylo-oligosaccharide, the pathogen that can carry attachment is excreted by enteron aisle, to anti- Only disease cluster in enteron aisle achievees the purpose that prevent diarrhea.Bifidobacterium utilizes xylo-oligosaccharide, generates a large amount of short-chain fat Acid can stimulate intestines peristalsis, promote gastroenteritic power, increase excrement wettability, and keep certain osmotic pressure, to prevent just It is secret.Liver function is protected, serum cholesterol is reduced, reduces blood pressure, immunity of organisms can be enhanced, anticancer promotes body to generate more The health-care efficacies (8) (9) such as kind nutriment, including vitamin B, niacin and folic acid.

Potassium sorbate be a kind of unsaturated fatty acid (salt) it can be absorbed by the metabolic system of human body and be decomposed into rapidly Carbon dioxide and water, in vivo noresidue.Mould, the activity of saccharomycete and aerobic bacteria, moreover it is possible to prevent can be effectively inhibited The harmful microbes such as clostridium botulinum, staphylococcus, salmonella are grown and breeding, but to anaerobic Bacillus and acidophilus breast bar The beneficial microbes such as bacterium are nearly unavailable, and the effect for restraining development is more stronger than bactericidal effect, effectively extends food to reach Holding time, and keep the flavor of original food.For the industries such as food, beverage, beverage dosage 0.5g/kg, anti-corrosion effect Fruit is 5-10 times of similar product sodium benzoate.Sorbic acid is the anti-of the highly effective and safe of international food and agricultural organization and Health Organisation recommendations Rotten antistaling agent, therefore it is preservative that this formula, which selects it,.

Feature of the present invention is the color of root Ju wine and beverage, fragrant, taste requirement, is coloured with color gorgeous hawthorn at prime；The day of hawthorn Right tart flavour, the fragrance of coloured malt improve drink quality.After the xylo-oligosaccharide for increasing appropriate amount, keep its sour-sweet tasty and refreshing, it is red gorgeous to lure People, acquirement are whetted the appetite at a glance, drink the good double excellent effects of mouthfeel.Therefore, product, which is made, in supplementary material selected in formula to reach To expected function of whetting the appetite.

The effect of combination of the above object adapted, relationship is clearly demarcated, reasonable recipe, and collaboration generates is safe and reliable, has digesting and appetizing, changes Kind stomach and intestine, promote the function of digestion.This matches the product of middle no incompatibility, safe to the human body.

2. preparation method of the present invention

2.1 crude drugs, which arrange, to be processed：

2.1.1 hawthorn, coloured malt, dried orange peel, stir-baked SEMEN RAPHANI, fennel seeds, fingered citron, sea-buckthorn, Radix Glycyrrhizae, cassia bark, meet《China People's republic's pharmacopeia》2010 editions regulations.Meet Ministry of Public Health's announcement《New resource food list and be both food and medicine Name of an article list》.

2.1.2 xylo-oligosaccharide meets QB/T 2984-2008 regulations, and potassium sorbate meets GB 13736-2008 regulations.

2.2 extraction：

According to large-fruited Chinese hawthorn whet the appetite formula ratio, weigh hawthorn, coloured malt, dried orange peel, stir-baked SEMEN RAPHANI, fennel seeds, fingered citron, sea-buckthorn, Radix Glycyrrhizae, cassia bark add 10 times of amount water, are heated to 70 DEG C, the immersions produced up to mechanical & electronic equipment corporation, Ltd using Shenzhen's remittance hundred million KESP-100KH ultrasonic extraction devices extract 3 times, extract about 50min every time；Filtering, merging filtrate.

2.3 concentration：

It is 1.20-1.30, pH3.0-4.0 that merging filtrate, which is concentrated under reduced pressure into relative density, obtains concentrate, concentrate is contained Enter in clean turnover barrel, indicates name of product, batch number, specification, quantity, operator's name, it is spare.

2.4 dispensing：

Product formula will be pressed in above-mentioned concentrate respectively, xylo-oligosaccharide, potassium sorbate is added, put into V-type blender, it After add 16 times of 38 degree of white wine or grape wine, 10min is mixed, it is ensured that uniformly.It stands 24 hours and precipitates, take supernatant It is put into filling machine.It is filling in glass wine bottle, every bottle of 250ml.

The originality of 2.5 techniques

It is us with reference to many Research Literatures for preparation process of the present invention, and after carrying out preferably, just determines following Technique：Ultrasonic wave extraction is to be applied to effective component of Chinese herbal medicine in recent years to extract a kind of newest more mature of separation Means.Its principle is mainly to accelerate the leaching of active ingredients of plants to extract using the cavitation of ultrasonic wave in a liquid.In addition, It also utilizes its sub-effect, such as mechanical oscillation, diffusion to smash, its acceleration is made to be extracted the diffusion of composition, release.Surpass according to statistics Sound wave is very high in 65--70 DEG C of working efficiency.And temperature spent at 70 DEG C in Chinese herbal medicine effective ingredient substantially not by It destroys.About 50 minutes (under the conditions of 70 degree) active ingredients of plants extraction times.And the digestion time of cooking process generally requires two By three hours.It is 3 times or more the times of ultrasonic wave extraction time.It is extracted per tank 3 times, the 90% of substantially extractable effective ingredient More than.

The method that traditional medicinal liquor is prepared is a lot of, and generally there are commonly following several at present：

2.5.1 cold-maceration：By the Chinese medicine thin slice or coarse crushing particle after processing, be placed in the container of sealing be added it is suitable Soaking in Chinese liquor 7-14 days or so, after active ingredient is dissolved into wine, you can filter out liquid and drink.But this method cannot carry completely Active ingredient is taken out, and time-consuming takes material.

2.5.2. hot dipping：Hot dipping is method that is a kind of ancient and effectively making medicinal liquor.Typically by Chinese medicine with Then wine lets cool storage with boiling certain time.This method can be accelerated to leach speed and the active ingredient in Chinese medicine is made more to hold Easily leach.But when boiling wine, alcohol burn should be prevented, prevents alcohol from volatilizing again.Therefore it has used less.

2.5.3. decocting method：Traditional Chinese medicine powder must be broken into powder by this method, be all put into marmite, add water to out powder about It 10 centimetres, impregnates about 6 hours, then heating is boiled 1-2 hours, and after filtering, the dregs of a decoction add water to be decocted 1 time again in right amount, is merged twice Liquid after standing 8 hours, then takes supernatant to be concentrated by heating into tune paste, after cooling, adds suitable wine, mixing is placed in In container, supernatant is taken after about 7 days,.But the armaticity Chinese medicine containing volatile oil should not use this method.

2.5.4. excise law：First add decocting to endure Chinese medicine, after filter and remove residue, be condensed into concoction, is placed in clean container In, it seals, sets at heat preservation 10 days or so, reduce the contact with air to the greatest extent, and keep certain temperature, filter residue after fermentation ?.But this method is time-consuming and perishable.

2.5.5. percolation：Percolation is produced suitable for pharmaceutical factory.Traditional Chinese medicine powder is first broken into thick end, suitable white wine is added Infiltration 2-4 hours, makes medicinal material coarse powder fully expand, is uniformly charged bottom by several times and is lined in the percolator of absorbent cotton, fills every time It is compressed with wooden stick after good.The handstone for installing Chinese medicine, covering gauze above, and press last layer clean；In order to avoid after white wine is added Medicinal powder is set to float.Then the switch for opening mouth under percolation filter, then adds white wine from percolator top at leisure, when liquid is from lower mouth Upper switch is closed when outflow, to make the liquid of outflow pour into percolator, continuously adds white wine to several centimeters of high drug powder Until, it is then capped, lower mouth switch is opened after placing 24-48 hours, percolate is made slowly to flow out.Liquid of filtering is collected by specified amount, Corrigent is added to stir evenly, is sealed after dissolving, filters out liquid after standing a few days, then add white wine to specified amount to get medicinal liquor.Still It is time-consuming and laborious, active ingredient cannot be extracted completely.

This product is planted according to having more advanced, ripe ultrasonic extraction process (10) (11) (12) using ultrasonic extraction Object active ingredient concentrates as medicinal extract, adds the wine or water of proportional quantity, and mixing is hooked to for wine or beverage.With method simplicity, save Power material saving, energy conservation and environmental protection can extract 95% or more active ingredient, be readily transported, it is only necessary to medicinal extract are transported to somewhere, in locality plus wine Add water, greatly reduce production cost, and can guarantee that the flavor of wine and beverage is constant, ensures that product quality, this method exist Belong to pioneering in the production technology of domestic plant liquor, we in State Intellectual Property Office's patent Chinese patent announces announcement systems with " ultrasonic extraction effective ingredients in plant is hooked to white spirit process " is to carry being retrieved, and result is：" the not content of this respect " is shown Show the patent that there is no this method at present.

Technical know-how：Radish seed frying technology is always to ensure the crux of radish seed effect, and raw radish seed is just as eating Raw turnip is equally irritant to stomach, can cause the noisy uncomfortable even vomiting of stomach.<<Chinese Pharmacopoeia>>Concocting method：A, radish Son：Crude drug is taken, impurity, cleaning, drying are removed.Used time smashs to pieces.B, stir-baked SEMEN RAPHANI：Net radish seed is taken, preheating frying container is set It is interior, it is heated, is fried to heaving with slow boiling, there is cracker, appearance color and luster to deepen, internal yellow, and when aromatic effusion, taking-up is dried in the air It is cool.Used time smashs to pieces.But how to fry that can be only achieved big production ideal effect be key technology.Radish seed is because its seed is tiny, when stir-fry It energy snuff, no significant color and luster variation cannot be not careful with regard to coking, duration and degree of heating selection is critically important thus as the seed of cowherb. Old medicine work is extended down to the time 15 to 20 minutes, radish seed cracks, and overflows according to repeatedly groping to be fried slowly with low baking temperature Warmth odor takes the dish out of the pot and spreads out.Plant skin fine fisssure at this time, be covered with tiny grey dot on the kind skin of rice-pudding red, benevolence not coke to get. But<<Chinese Pharmacopoeia>>With old medicine work experience entirely be experience, without quantizating index, it is difficult to run in the industrial production.We By being studied repeatedly using micro-wave oven frying method：3.5min is heated in microwave big fire.It can reach and heave, there is cracker, appearance color Pool is deepened, internal yellow, and aromatic effusion ideal effect.It is that last stomach of taking is very comfortable to crush, smell fragrant, and entrance is micro- peppery It is very fragrant.This technology solves stir-baked SEMEN RAPHANI technical barrier for many years, creative.

For preferred preferable technique, we publish according to Ministry of Public Health's health legal system with supervision department using general flavone as index《It protects Health food is examined and assessment technique specification》(2003) assay method of total flavonoids in health food in, to following 6 batch sample point Comparative study of the Different Extraction Method to general flavone content has not been carried out, it is as a result as follows：

Comparative study of the Different Extraction Method to general flavone content

Lot number	The name of an article	General flavone content in per 100ml
			2013X0287	Compound large-fruited Chinese hawthorn ultrasound alcohol extract	184.0mg/100ml
2013X0290	Compound large-fruited Chinese hawthorn ultrasound Aqueous extracts	182.5mg/100ml
			2013X0291	Compound large-fruited Chinese hawthorn water cooking liquid	14.2mg/100ml
2013X0288	Single hawthorn ultrasound alcohol extract	27.8mg/100ml
			2013X0289	Single hawthorn ultrasound Aqueous extracts	25.4mg/100ml
2013X0292	Single hawthorn water cooking liquid	1.0mg/100ml

The above results show that：General flavone content 184.0mg in the every 100ml of large-fruited Chinese hawthorn alcohol ultrasound alcohol extract, large-fruited Chinese hawthorn ultrasound General flavone content 182.5mg in the every 100ml of Aqueous extracts, but the two statistics no significant difference, large-fruited Chinese hawthorn water cooking liquid are only 14.2mg has notable difference with ultrasonic extraction.

Single hawthorn ultrasound alcohol extract 27.8mg, single hawthorn ultrasound Aqueous extracts 25.4mg, the two statistics is without apparent poor Different, single hawthorn water cooking liquid is only 1.0mg, has notable difference with ultrasonic extraction.Illustrate that ultrasonic extraction is substantially better than traditional water decoction Method, carries at low cost in view of ultrasonic water, therefore it is this production technology to select ultrasonic water extraction.

Specific implementation mode

Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention Rather than it limits the scope of the invention.In the following examples, the experimental methods for specific conditions are not specified, usually according to conventional strip Part or according to the normal condition proposed by manufacturer.Unless otherwise stated, otherwise all percentage, ratio, ratio or number is pressed Weight meter.

Unless otherwise defined, all professional and scientific terms used in text and meaning known to one skilled in the art Justice is identical.In addition, any method and material similar or impartial to described content can be applied to the method for the present invention.Wen Zhong The preferred implement methods and materials are for illustrative purposes only.

Large-fruited Chinese hawthorn appetizing concentrate promotes digestive function Report on Animal (selected parts)

1. test material

1.1 tested material：Large-fruited Chinese hawthorn concentrate is (referred to as：Appetizing drink) 310ml/ bottles, per 1000ml by hawthorn, coloured malt, stir-fry The total 100g compositions such as radish seed, dried orange peel, healthcare function are to promote digestion (being used for indigestion).310ml/ bottles of population dose, 1 Bottle/time, 2 times/day, amount to 62g raw materials.Adult is calculated as 1.03g raw materials/kg weight by 60kg weight.

Submitted sample is packed with 1.5L closed plastic boxes, and content is that sepia concentrates thick liquid, and No. 1 side is 7.53g Raw material/g, No. 2 sides are 9.12g raw materials/g, and product batch number (generation date of application) 20131115 is provided, this laboratory 4 by Hu Baihu It is DEG C stored refrigerated.

When using mouse test, directly extracts concentrate and distilled water is added to be diluted to 1.55g raw materials/ml for high dose respectively Group takes high dose group 4ml that distilled water 8ml is added to be diluted to 0.52g raw materials/ml for middle dose group, middle dose group 4ml is taken to add distillation Water 4ml is diluted to 0.26g raw materials/ml for low dose group, gavage (ig) volume 0.2ml/10g BW.

When using rat test, directly extracts concentrate and distilled water is added to be diluted to 3.1g raw materials/ml for high dose respectively Group takes high dose group 20ml that distilled water 40ml is added to be diluted to 1.0g raw materials/ml for middle dose group, middle dose group 15ml is taken to add steaming Distilled water 15ml is diluted to 0.5g raw materials/ml for low dose group, ig volume 1.0ml/100g BW.

1.2 model agent：R-1132, every content 2.5mg, lot number 201102014.

It is prepared by 1.3 chemical reagent：Prepared Chinese ink is pasted；0.05mol/L hydrochloric acid solutions；2cm egg white glass capillaries；Ether, physiology Brine, Iodophor, 75% ethyl alcohol.

1.4 animal：SPF grades of SD kinds female rats 56,130~150g of weight；KM kinds female mice 80, weight 20~ 22g is provided, animal productiong licensing number by National Institute for Food and Drugs Control's Laboratory Animal Resource center：SCXK (capital) 2009-0017.Large and small mouse sub-cage rearing, per 4 or 10, cage, every group of animal 8 or 10, breeding observing is after 7 days or 3 days For testing.

1.5 laboratory animal room：For barrier environment, totally-enclosed state, three-stage filtration is blown, and 22~24 DEG C of temperature is relatively wet Degree 40~45%, fluorescent lamp illuminate for 12 hours, and experimental animal uses credit number：SYXK (capital) 2011-0008.High pressure sterilization Wood wool bits are bedding and padding.Plastics mouse box body accumulates 35 × 30 × 17cm (rat) or 28 × 17 × 12cm (mouse), stainless steel wire top Lid.SPF grades of big and small mouse upgrowth breeding feeds are pulled together feed corporation,Ltd's production by Beijing Australia of section, and it is qualified that Feedstuff Enterprises examine Card number：Capital, which is raised, examines (2009) 06166, product batch number：13103123.Laboratory animal room of the court provides purified water and freely drinks.

1.6 key instruments and instrument：1/10 animal electronic balance of sensibility reciprocal, 1/1000 electronic balance of sensibility reciprocal, 37 DEG C of constant temperature trainings Case is supported, electric furnace anaesthetizes tank, timer, vernier caliper, 1m straight steel rulers, small grinding wheel, glass plate, diameter 0.8~1.0mm capillary glass Glass pipe, 100ml mortars, 50ml beakers, 25ml Tissue Culture Flasks 60,1ml, 5ml, 10ml disposable syringe, large and small mouse solution Cut open plate, suture silk, haemostatic clamp, medical scissors and tweezers etc..

2. method and result

The measurement of 2.1 rat body weights and food ration

Experiment is divided into 7 groups, and (solvent control group, No. 1 side's 30.9g/kg group of appetizing drink, 10.3g/kg groups and 5.2g/kg groups are opened Stomach drinks No. 2 side's 30.9g/kg groups, 10.3g/kg groups and 5.2g/kg groups), every group of 8 rats.Daily ig 1 time, continuous 30 days, gives Rat has no loose stools or does just, no Systemic reaction after giving tested material.Every rat of each group is weighed weekly 2 times, per cage Rat claims weekly feed total amount and surplus 2 times.It calculates the average weight of every group of rat of each period, weight gain value, take the photograph Appetite and food utilization.The results detailed in Table 1~4.

The observation of the appetizing drink of table 1 all weight each to rat

Note：The p compared with solvent control group>0.05.

The observation of the appetizing drink of table 2 all weight gain values each to rat

Note：The p compared with solvent control group>0.05.

The observation of the appetizing drink of table 3 all food rations each to rat

Note：The p compared with solvent control group>0.05.

The observation of the appetizing drink of table 4 all food utilizations each to rat

Note：The p compared with solvent control group>0.05.

The result shows that：Appetizing No. 1 side of drink and No. 2 each dosage groups in side increase rat body weight, weight during giving tested material Value added, food ration and food utilization, which have no, to be obviously increased, compared with solvent control group, statistical analysis unknown significance difference (p<0.05)。

The measurement of 2.2 rat gastric juice and protease

Experiment is divided into 7 groups, and (solvent control group, No. 1 side's 30.9g/kg group of appetizing drink, 10.3g/kg groups and 5.2g/kg groups are opened Stomach drinks No. 2 side's 30.9g/kg groups, 10.3g/kg groups and 5.2g/kg groups), every group of 8 rats.Daily ig 1 time, continuous 30 days, gives Rat has no loose stools or does just, no Systemic reaction after giving tested material.30th day ig gives fasting 24 hours after tested material, In fasting, ig gives tested material again or distilled water is primary within 20 hours.Etherization rat after 4 hours, surgical incision stomach wall, With suture silk ligation pylorus (channel for closing gastric content downlink), suturing them notch at stomach and duodenum.It is 5 small When after cervical dislocation put to death rat, open abdominal cavity, haemostatic clamp clamps at esophagus hypomere cardia, the complete full stomach of clip, and by stomach content Object all takes out, and measures the gastric secretion total amount of every rat, calculates gastric juice discharge rate per hour.1ml supernatant gastric juice is taken to add again Enter 15ml 0.05mol/L hydrochloric acid solutions mixing in 25ml Tissue Culture Flasks, be put into freshly prepared 2 radixin pipe, closes bottle Mouthful, it sets in 37 DEG C of constant incubators and is incubated 24 hours, protein pipe is taken out, with each protein pipe both ends hyalomere of vernier caliper measurement Point length, pepsin activity and pepsin discharge rate are calculated with the average value of the transparent length in 4 ends.Formula is as follows：

Pepsin activity (u/ml)=(average values of four end protein pipe transparent part length)²×16

The gastric juice amount of pepsin discharge rate (u/h)=pepsin activity × per hour

The results detailed in Table 5,6.

Observation of the appetizing drink of table 5 to rat gastric secretion

Note：The * P compared with solvent control group<0.05；**P<0.01.

Influence of the appetizing drink of table 6 to rat pepsin

Note：The * P compared with solvent control group<0.05；**P<0.01.

The result shows that：The square high dose group of appetizing drink 1 has gastric juice total amount, gastric juice discharge rate and pepsin discharge rate bright Aobvious to increase, middle dose group is significantly increased pepsin discharge rate；The square high dose group of appetizing drink 2 is to gastric juice total amount, gastric juice Discharge rate, pepsin activity and pepsin discharge rate are significantly increased, and middle dose group has obviously pepsin discharge rate Increase；For the above numerical value compared with solvent control group, statistical analysis has significant difference or significant differences (p<0.05 Or<0.01).

2.3 mouse small intestine exercise tests

Experiment be divided into 8 groups (solvent control group, model control group, appetizing drink No. 1 side's 30.9g/kg group, 10.3g/kg groups and 5.2g/kg groups, No. 2 side's 30.9g/kg groups of appetizing drink, 10.4g/kg groups and 5.2g/kg groups), every group of 10 mouse.Daily ig 1 Secondary, continuous 15 days, mouse had no loose stools or does just that no Systemic reaction, each group mouse weight increases after giving tested material It is normal.Tested material is given to each dosage group after 16 hours within 15th day, to solvent control group and model comparison again in fasting Group gives isometric distilled water；After 30 minutes in addition to solvent control group, every mouse ig gives model agent compound diphenoxylate 5mg/kg (suitable people dosage 20X), after giving model agent 30 minutes, each group gives prepared Chinese ink and pastes 0.6ml/ weight；After 25 minutes Cervical dislocation puts to death mouse, separation abdominal viscera of cutting open the belly, the complete gastrointestinal tissue of clip, and carefully cuts mesostenium, makes small Intestines slowly stretch, and on spilling the glass plate of physiological saline, stretch small intestine, and after bouncing back naturally, measurement is pasted from pylorus~prepared Chinese ink The distance (prepared Chinese ink paste promotes length) in forward position and the distance (total small intestinal length) from pylorus~colon section start, calculate prepared Chinese ink paste and push away Conversion value is promoted into rate and prepared Chinese ink pasteFormula is as follows：

Prepared Chinese ink pastes propulsion rate (%)=prepared Chinese ink paste and promotes length (cm) ÷ total small intestinal lengths (cm) × 100%.The results detailed in Table 7.

The observation that the appetizing drink of table 7 acts on mouse small intestine movement

Note：The #p compared with solvent control group<0.05；The * p compared with model control group<0.05.

The result shows that：The small intestine prepared Chinese ink of model control group and each dosage group of tested material paste propulsion rate obviously slows down；With solvent Control group compares, and statistical analysis all has significant difference (p<0.05).Appetizing No. 1 side of drink and No. 2 sides are low, middle dose group Small intestine push speed variation unobvious compared with model control group, no difference of science of statistics (p>0.05)；No. 1 side and No. 2 are drunk in appetizing The small intestine ink progradation and prepared Chinese ink of square high dose group promote conversion value to be then significantly increased, compared with model control group, statistics Credit analysis has significant difference (p<0.05).

3. conclusion

Appetizing No. 1 side of drink and No. 2 sides 15 days or 30 days are given with KM kinds mouse and SD kind rat continuous gavages, it is each to rat Zhou Tichong, weight gain value, food ration and food utilization have no significant change.No. 1 side's 30.9g/kg high dose group is to gastric juice Total amount, gastric juice discharge rate and pepsin discharge rate tool have significantly increasing action, and have to the slow model of mouse gastrointestinal peristalsis bright Aobvious small intestine push speed increasing action；10.3g/kg middle dose groups have significantly increasing action pepsin discharge rate.No. 2 sides 30.9g/kg high dose groups are significantly increased gastric juice total amount, gastric juice discharge rate, pepsin activity and pepsin discharge rate Effect has apparent small intestine push speed increasing action to the slow model of mouse gastrointestinal peristalsis；10.3g/kg middle dose groups are to stomach Protease discharge rate has significantly increasing action.

Prompt：Appetizing No. 1 side of drink and No. 2 sides can increase gastric secretion and pepsin in 30.9g/kg dosage to rat Discharge, alleviates and the mouse gastrointestinal peristalsis mitigated caused by compound diphenoxylate model agent is slow, to promotion digestive function Effect.The every experimental data in appetizing No. 1 side of drink and No. 2 sides relatively has no notable difference.

The acute toxicity test report (selected parts) of large-fruited Chinese hawthorn appetizing drink (concentrate)

1. test material

1.1 tested material：The red appetizing drink of mountain wheat is (referred to as：Appetizing drink) 310ml/ bottles, per 1000ml containing hawthorn, coloured malt, stir-fry The total 100g compositions such as radish seed, dried orange peel, healthcare function are to promote digestion (being used for indigestion).310ml/ bottles of population dose, 1 Bottle/time, 2 times/day, amount to 62g raw materials.Adult is calculated as 1.03g raw materials/kg weight by 60kg weight.

Tested material is packed with 1.5L closed plastic boxes, and content is that sepia concentrates thick liquid, and No. 1 side is 7.53g former Material/g, No. 2 sides are 9.12g raw materials/g, and product batch number (generation date of application) 20131115 is provided, 4 DEG C of this laboratory by Hu Baihu It is stored refrigerated.

Before experiment, it is dilute that No. 1 square concentrate (9.3g raw materials/ml) with No. 16 gavage syringe needles and 1ml syringes is not added with distilled water It releases and directly extracts gavage (ig)；No. 2 square concentrates (11.8g raw materials/ml) need to add distilled water to be diluted to 10.2g raw materials/ml, then use No. 16 gavage syringe needles and 1ml syringes extract.Mouse ig volume 0.2ml/10g BW.

1.2 animal：SPF grades of KM kind mouse, totally 40, female, fifty-fifty, the 20~22g of weight of hero, experimental animal production licence Number：SCXK (capital) 2009-0017 is provided by National Institute for Food and Drugs Control's Laboratory Animal Resource center.Mouse presses gender Per 10, cage, for testing after laboratory animal room's breeding observing 3 days.Mouse fasting 16 hours, can't help water before experiment.

1.3 laboratory animal room：For barrier environment, totally-enclosed state, three-stage filtration is blown, and 22~24 DEG C of temperature is relatively wet Degree 40~45%, fluorescent lamp illuminate for 12 hours, and experimental animal uses credit number：SYXK (capital) 2011-0008.High pressure sterilization Wood wool bits are bedding and padding.The small mouse-cage body of plastics accumulates 29 × 18 × 12cm, stainless steel wire head cover.SPF big and small mouse upgrowth breeding feeds It is provided for the feed corporation,Ltd that pulls together of Beijing Australia of section, examines quality certification number：Capital, which is raised, examines (2009) 06166, product batch number： 13103123.Laboratory animal room of the court provides purified water and freely drinks.

1.4 key instruments and instrument

1/10 animal electronic balance of sensibility reciprocal, 1/1000 electronic balance of sensibility reciprocal, 50ml beakers, 1ml disposable syringes, dissection Plate, medical scissors and tweezers.

2. test method

The mouse of fasting 16 hours is taken to be divided into 2 groups, each 10 of every group of male and female.Tested material is given according to every mouse weight ig Suspension is given at 2 times (intervals 5 hours) in one day, and No. 1 side's group accumulation is 372g raw materials/kg BW to sample amount；No. 2 side's group accumulations It is 408g raw materials/kg BW to sample amount.Give the adverse reaction that animal on the 14th is observed continuously after tested material and death state.Record Give sample 0,3,7 days and 14 days mouse weight.

3. result

Same day ig is observed continuously 4 hours after giving tested material, and mouse is in high spirits, and behavior is normal, activity freely, respiratory rate Unchanged, stimulation weak to the sound etc. is sensitive, and listless excited or inhibition behavior has no loose stools, the urinary incontinence, mouth, nosebleed and eye Conjunctival congestion etc..When giving tested material crawl mouse for the second time, it is seen that black soft stool is discharged, and has no that other toxic reactions occur. Next day mouse hair is moist, and freely, eupnea, reaction is quick for activity, and food consumption, drinking-water etc. are normal, there is black excrement Discharge.Mouse on the 14th is observed continuously and does not find any new abnormal response or death.Survival mice average weight increases in the meantime Long 10~16g.Refer to table 1.

The observation that the appetizing drink of table 1 reacts Mouse Acute Toxicity

Full 14 days cervical dislocations of contamination put to death mouse, dissect each internal organs and carry out gross examination of skeletal muscle：Brain is without bleeding and ponding；Thoracic cavity without Bloody fluid, the heart, lung not enlargement, no hemostasis；Abdominal cavity without hydrops, each organ adhesion, liver,spleen,kidney surface is smooth, not enlargement, Without tubercle, food is more in stomach, and enteric cavity is without flatulence, colon and rectum there are excrement, and each histoorgan is complete, color and luster is ruddy, not See the abnormalities such as enlargement, mass, ulcer, atrophy, necrosis, hyperemia and hemostasis.

It is 1.03g raw materials/kg BW that adult calculates oral dose by 60kg weight.No. 1 side's group accumulation is 372g former to sample amount Material/kg BW is equivalent to 360 times of clinical people's dosage；No. 2 side's group accumulations are 408g raw materials/kg BW to sample amount, are equivalent to clinic 396 times of people's dosage.

4. conclusion

2 times a day ig's KM kinds mouse contaminates, and is observed continuously 14, has no overt toxicity reaction and death.Its LD₅₀Amount> 372g/kg, according to acute toxicity (LD₅₀) dose fractionation standard belongs to nontoxic rank.

The preparation method of the composition beverage and chewable tablets is expanded on further by the following examples：

Embodiment 1

The preparation method of large-fruited Chinese hawthorn beverage for improving appetite (formula 1)：

1.1 crude drugs, which arrange, to be processed：

1.1.1 hawthorn, coloured malt, dried orange peel, stir-baked SEMEN RAPHANI, fennel seeds, fingered citron, sea-buckthorn, Radix Glycyrrhizae, cassia bark, meet《Middle Chinese People republic pharmacopeia》2010 editions regulations.Meet Ministry of Public Health's announcement《New resource food list and be both food and drug List》

Dosage is 50 parts of hawthorn, 15 parts of coloured malt, 10 parts of dried orange peel, 20 parts of stir-baked SEMEN RAPHANI, 8 parts of fennel seeds, 8 parts of fingered citron, sand 15 parts of spine, 8 parts of Radix Glycyrrhizae, 8 parts of cassia bark, 5 parts of xylo-oligosaccharide, 0.5 part of potassium sorbate.

1.1.2 xylo-oligosaccharide meets QB/T 2984-2008 regulations, and potassium sorbate meets GB 13736-2008 regulations.

1.2. extraction：

According to large-fruited Chinese hawthorn beverage for improving appetite formula, hawthorn, coloured malt, dried orange peel, stir-baked SEMEN RAPHANI, fennel seeds, fingered citron, sand are weighed Spine, Radix Glycyrrhizae, cassia bark add 10 times of amount water, are heated to 70 DEG C, the immersions produced up to mechanical & electronic equipment corporation, Ltd using Shenzhen's remittance hundred million Formula KESP-100KH ultrasonic extraction devices extract 3 times, extract about 50min every time；Filtering, merging filtrate.

1.3 concentration：

1.4 dispensing：

This operating post is D grades of clean areas.Check the verification concentrate intermediate name of an article, specification, lot number, quantity.It will be above-mentioned Product formula is pressed in concentrate respectively, xylo-oligosaccharide, potassium sorbate, 16 times of purified water is added, heated 30 minutes, stand 24 hours Precipitation, filtration are contained after the assay was approved in clean turnover barrel, and name of product, batch number, specification, quantity, operator are indicated Name, is transported to subsequent processing in time, bucket housing cleaning polybag when transhipment.

1.5 filling：

This operating post is D grades of clean areas.The liquid prepared is transported to bottling department, is carried out filling, filling outside in draw ring Pop can (310ml/ tanks) sealing, the sterilizing opened.Indicate name of product, batch number, specification, quantity, operator's name.

1.6 outer packing：Outer packing uses corrugated case.

1.7 examining：It tests according to company standard.

1.8 storage：Product through that can be put in storage after the assay was approved.

Embodiment 2

The preparation method of large-fruited Chinese hawthorn appetizing chewing tablet and its preparation technology：

2.1 crude drugs, which arrange, to be processed：

2.1.1 hawthorn, coloured malt, dried orange peel, stir-baked SEMEN RAPHANI, fennel seeds, fingered citron, sea-buckthorn, Radix Glycyrrhizae, cassia bark, meet《Middle Chinese People republic pharmacopeia》2010 editions regulations.Meet Ministry of Public Health's announcement《New resource food list and be both food and drug List》.

2.2. extraction：

According to large-fruited Chinese hawthorn appetizing chewing tablet and its preparation technology formula, hawthorn, coloured malt, dried orange peel, stir-baked SEMEN RAPHANI, fennel seeds, fingered citron, sand are weighed Spine, Radix Glycyrrhizae, cassia bark add 10 times of amount water, are heated to 70 DEG C, the leachings produced up to mechanical & electronic equipment corporation, Ltd using Shenzhen's remittance hundred million Enter formula KESP-100KH ultrasonic extraction devices, extract 3 times, extracts about 50min every time；Filtering, merging filtrate.

2.3 concentration：

3.4 dispensing：

This operating post is D grades of clean areas.Check the verification concentrate intermediate name of an article, specification, lot number, quantity.It will be above-mentioned Product formula is pressed in concentrate respectively, xylo-oligosaccharide, potassium sorbate is added, 16 times of starch is then added, contains into clean turnover barrel In, 10min is mixed, it is ensured that uniformly, make dough.Indicate name of product, batch number, specification, quantity, operation Person's name, is transported to subsequent processing in time, bucket housing cleaning polybag when transhipment.

1.5 film-making：

This operating post is D grades of clean areas.The dough drug prepared is transported to film-making workshop, carries out film-making, every weight 1g.100 every bottle, sealing, sterilizing.Indicate name of product, batch number, specification, quantity, operator's name.

1.6 outer packing：Outer packing uses corrugated case.

1.7 examining：It tests according to company standard.

Embodiment 3

The preparation method of large-fruited Chinese hawthorn aperitif (formula 2)：

3.1 crude drugs, which arrange, to be processed：

3.1.1 hawthorn, coloured malt, dried orange peel, stir-baked SEMEN RAPHANI, fennel seeds, fingered citron, sea-buckthorn, Radix Glycyrrhizae, cassia bark, Himalayan mayapple fruit, the stem of noble dendrobium Meet《Pharmacopoeia of People's Republic of China》2010 editions regulations.Meet Ministry of Public Health's announcement《New resource food list and both Be food it is drug list again》.

Dosage is 50 parts of hawthorn, 15 parts of coloured malt, 10 parts of dried orange peel, 20 parts of stir-baked SEMEN RAPHANI, 8 parts of fennel seeds, 8 parts of fingered citron, sand 15 parts of spine, 8 parts of Radix Glycyrrhizae, 8 parts of cassia bark, 5 parts of Himalayan mayapple fruit, 3 parts of the stem of noble dendrobium, 5 parts of xylo-oligosaccharide, 0.5 part of potassium sorbate.

3.1.2 xylo-oligosaccharide meets QB/T 2984-2008 regulations, and potassium sorbate meets GB 13736-2008 regulations.

3.2 extraction：

According to large-fruited Chinese hawthorn whet the appetite formula ratio, weigh hawthorn, coloured malt, dried orange peel, stir-baked SEMEN RAPHANI, fennel seeds, fingered citron, sea-buckthorn, Radix Glycyrrhizae, cassia bark, Himalayan mayapple fruit, the stem of noble dendrobium add 10 times of amount water, are heated to 70 DEG C, extract 3 times, extract about 50min every time；Filtering merges Filtrate.

3.3 concentration：

It is 1.20-1.30, pH3.0-4.0 that merging filtrate, which is concentrated under reduced pressure into relative density, obtains concentrate.

3.4 dispensing：

The foregoing is merely illustrative of the preferred embodiments of the present invention, is not limited to the substantial technological content model of the present invention It encloses, substantial technological content of the invention is broadly to be defined in the right of application, any technology that other people complete Entity or method also or a kind of equivalent change, will if identical with defined in the right of application It is considered as being covered by among the right.

Bibliography

(1) Zhejiang the Chinese medicine progress Journal of Traditional Chinese Medicine of Zhang Xiaofeng functional dyspepsia FDs, 2001 (07)：14-16

(2) Liu Jianping, Xie Wei, Dai Yehong, Lin Mei, Zhao Hongwei, the research of Kang Wei Chinese medicine functional dyspepsia FDs The Hebei present situation traditional Chinese medicine journal, 2006 (03)：45-48

(3) Xue Jie, summer prosperous hawthorn pharmacological research progress, Xinjiang traditional Chinese medicine, 2002,20 (4)：69.71

(4) Luo Yumei, Wang Hezhen.Treasure's traditional Chinese medical science traditional Chinese medicines when chemical composition and pharmacological research the progress of hawthorn, 2004,15 (1)：53-54

(5) the research overview Chinese medicines 2010 (09) of Wang Xiong, Wu Jin tiger fructus hordei germinatus：1503-1508

(6) Zhao Xiu tinkling of pieces of jade dried orange peels physiologically active ingredient progress food industry science and technology, 2013,12 phase 376-381)

(7) Li Hong radish seeds modern study overview (summary) Beijing University of Chinese Medicine journal, 2001 (03)：67-69

(8) Yin Liduan, Wang Lizhi, Dong apply farming industries from super xylo-oligosaccharides in functional food, .2003,6：.29-31

(9) Zhang Darong, Yin Ji increase clinical application food science and technology of the xylo-oligosaccharides in improving function of intestinal canal disorder, 2007,10：125-126.

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Claims

1. a kind of large-fruited Chinese hawthorn aperitif, it is characterised in that it is made of the raw material of following weight proportion：20-70 parts of hawthorn fries wheat 7-20 parts of bud, 5-15 parts of dried orange peel, 10-30 parts of stir-baked SEMEN RAPHANI, 5-10 parts of fennel seeds, 5-10 parts of fingered citron, 10-20 parts of sea-buckthorn, Radix Glycyrrhizae 6-12 parts, 5-10 parts of cassia bark, 2-10 parts of xylo-oligosaccharide, 0.3-0.6 parts of potassium sorbate.

2. a kind of large-fruited Chinese hawthorn aperitif according to claim 1, it is characterised in that it by following weight proportion raw material system At：50 parts of hawthorn, 15 parts of coloured malt, 10 parts of dried orange peel, 20 parts of stir-baked SEMEN RAPHANI, 8 parts of fennel seeds, 8 parts of fingered citron, 15 parts of sea-buckthorn, Radix Glycyrrhizae 8 Part, 8 parts of cassia bark, 5 parts of xylo-oligosaccharide, 0.5 part of potassium sorbate.

The composition 3. a kind of large-fruited Chinese hawthorn is whetted the appetite, it is characterised in that it is made of the raw material of following weight proportion：20-70 parts of hawthorn, 7-20 parts of coloured malt, 5-15 parts of dried orange peel, 10-30 parts of stir-baked SEMEN RAPHANI, 5-10 parts of fennel seeds, 5-10 parts of fingered citron, 10-20 parts of sea-buckthorn, 6-12 parts of Radix Glycyrrhizae, 5-10 parts of cassia bark, 2-10 parts of xylo-oligosaccharide, 0.3-0.6 parts of potassium sorbate.

4. a kind of large-fruited Chinese hawthorn is whetted the appetite composition according to claim 3, it is characterised in that it by following weight proportion raw material It is made：It is 50 parts of hawthorn, 15 parts of coloured malt, 10 parts of dried orange peel, 20 parts of stir-baked SEMEN RAPHANI, 8 parts of fennel seeds, 8 parts of fingered citron, 15 parts of sea-buckthorn, sweet 8 parts of grass, 8 parts of cassia bark, 5 parts of xylo-oligosaccharide, 0.5 part of potassium sorbate.

5. a kind of preparation method of large-fruited Chinese hawthorn beverage for improving appetite, which is characterized in that a kind of large-fruited Chinese hawthorn described in claim 3 or 4 16 times of purified water is added in appetizing composition, heats 30 minutes, stands 24 hours and precipitates, filtration is to get large-fruited Chinese hawthorn beverage.

6. a kind of preparation method of large-fruited Chinese hawthorn appetizing preparation, which is characterized in that a kind of large-fruited Chinese hawthorn described in claim 3 or 4 It is acceptable that clinical human is added in appetizing composition《Pharmacopoeia of People's Republic of China》It is required when the different preparations of license are at agent Various customary adjuvants to get.