CN101244157A - Natural plants extract, preparation method and application thereof - Google Patents

Abstract

The invention provides a natural plant extract, a preparation method and the application. The invention is characterized in that: the natural plant extract is prepared by grape skin, haw apple, lotus leaf, medlar, sea buckthorn, fiveleaf gynostemma herb, pilose asiabell root, radix polygoni multiflori preparata and other materials, and the materials are processed solvent extract, machining and refining and then the natural plant extract is obtained; the preparation method comprises A individual extraction method and B mixed extraction method. The natural plant extract has the advantages of effectively controlling energy absorbing of bodies, reducing fat accumulation in vivo and reducing harm of overnutrition to bodies, reducing blood fat, purifying blood, controlling blood sugar, interdicting and reversing the development of sub-healthy state, reducing morbidity risk of hyperlipemia, hypertension, diabetes, coronary heart disease, fatness, fatty liver and other diseases, providing healthy population for eating to keep fit, also providing sub-healthy population for eating to effectively interdict and reverse the development of sub-healthy state, still being used to prepare the medicament for curing hypertension, hyperlipemia, diabetes and other diseases after deep processing.

Description

A kind of natural plant extracts and its production and application

Technical field:

The present invention relates to a kind of natural plant extracts and its production and application, be specifically related to a kind of natural plant extracts and its preparation method that from raw materials such as Pericarpium Vitis viniferae, haw berry, Folium Nelumbinis, Fructus Lycii, Fructus Hippophae, Herb Gynostemmae Pentaphylli, Radix Codonopsis, Radix Polygoni Multiflori Preparata, extracts, and the application of this extract in the preparation of food, food additive, health food, medicine.

Technical background:

Usually, people only notice that malnutrition can bring health problem, have but ignored overnutrition to healthy harm.The archaism cloud: " having too much of a good thing ", nutrition also are like this.High-energy, high protein, higher fatty acid, low dietary fiber are the diet structure of " three-hypers one is low " of characteristics, though nutritious, but people's health in serious harm.World Health Organization (WHO) in 2000 announces: the number of whole world overnutrition death has surpassed first because malnutrition and dead number.This survey result has beaten alarm bell for global people.In China, the particularly golden collar of some booming incomes of most of town dwellers is arranged, white collar clan's dietary habit is along with huge transformation has also taken place in the increase of income, they no longer follow Chinese traditional meals custom of " based on vegetable protein; animal proteinum is auxilliary ", the animal proteinum of the substitute is with " higher fatty acid; high protein; high-energy " is the diet of master so-called " western civilized life ", tend to select meat or food rich in fat, and it is comparatively exquisite, too sweet food, but the food of high fiber absorbs very little, thereby cause fatty intake in their diet, the increase of heat energy intake, form overnutrition and cause the imbalance of diet structure, adding physical exertion reduces, operating pressure increases the weight of, and environmental pollution etc., all of these factors taken together all becomes hyperlipemia, hypertension, diabetes, cardiovascular and cerebrovascular disease, fat, cancer, the direct or indirect factor that kidney disease and the prevalence of chronic diseases relevant with nutrition obviously increase.Fast food and so-called " the western civilized life " of these " energy bombs " or " junk food " that are called by nutritionist become the arch-criminal who causes " modern civilization disease " to rise year by year just!

" modern civilization disease " also usually is called " affluenza " by people, be the general name that comprises a series of diseases such as hyperlipidemia, hypertension, high blood viscosity, diabetes, arteriosclerosis, cardiovascular and cerebrovascular disease, fatty liver, obesity.Along with enriching of human material life, the disease of these serious harm human body health and the sickness rate of disease increase year by year, have brought a lot of troubles for human live and work.In " new affluenza " seminar of holding in recent years, hypertension, diabetes, blood fat disorder, obesity, any three kinds take place simultaneously in these four kinds of diseases, just become " new affluenza ", because it causes dead probability such as heart and brain accident higher, therefore be called as " dead quartet ".The expert points out, though " new affluenza " is more common in middle-aged and elderly people, but the average generation age of Developmental and Metabolic Disorder in fact from one's late 30s just, prevent " new affluenza ", key is early prevention, produce, occur Developmental and Metabolic Disorder in a large number from free radical, just should prevent pointedly and treat.The disease that World Health Organization (WHO) is relevant with these is defined as a kind of epidemic disease, also it has been classified as with drug abuse, gun in the U.S. and has spread unchecked the social problem that is equal to.Why the World Health Organization (WHO) and the U.S. do like this, are because between the multiple diseases such as hyperlipidemia, hypertension, high blood viscosity, diabetes, obesity, cholecystitis, osteoarthritis, coronary heart disease, breast carcinoma, colon cancer extremely close relation is arranged.The statistical data of World Health Organization (WHO) shows: annual about 1,000,000 people in the whole world are fat ranks because of overnutrition adds.In China, because there are 1.6 hundred million adult's dyslipidemia in the overnutrition whole nation, other has 1.6 hundred million adults to suffer from hypertension, and more than 2,000 ten thousand people suffer from diabetes.China has 2,000,000 people to die from cardiovascular disease to account for the national cause of the death first place every year approximately, and wherein urban population cardiovascular and cerebrovascular disease mortality rate reaches 38%; While overweight people's onset diabetes rate is 5 times of normal type.The medical treatment cost in hospital of above-mentioned chronic disease such as hypertension, diabetes is 827.3 hundred million yuans, and the chronic disease relevant with diet nutritional accounted for 70% of the cause of death.Scholarly forecast is arranged, and in following 20 years, China will face the high-incidence season of hyperlipidemia, hypertension, hyperglycemia, high blood viscosity, obesity and apoplexy.These diseases eat basically.Today, we should " how eating ", and the problem that be not only transformation of customs and usages, changes lifestyles also becomes the social problem that is worth showing great attention to day by day!

The healthy of healthy population, sub-health population in the dietary habit serious threat of " high-energy, high protein, higher fatty acid ", and the troop of modern civilization disease grows stronger day by day.So some people begins to go on a diet or be a vegetarian, wish to reduce caloric absorption by such method, reduce the accumulation of fat, reduce the generation of diseases such as hyperlipemia, hypertension, diabetes.But going on a diet and being a vegetarian not is to be best choice, and can accomplish not to be an easy thing through and through yet! Its reason mainly contains three: at first, and through investigation with studies show that: too go on a diet or emphasize vegetarian diet, can increase the occurrence risk of disease owing to nutrition is unbalanced.People's life just is maintained by taking in various necessary nutrients, when excessively going on a diet or emphasizing vegetarian diet, the nutrient that body is taken in must reduce, can not satisfy the needs of body, cause nutrient unbalanced, thereby bring out cholelithiasis, osteoporosis, alopecia, function of intestinal canal disorder easily, make that brain cell is impaired, amnesia, amenorrhea etc., but also can increase the incidence rate of cardiovascular and cerebrovascular disease.Secondly, diet is human never weak consume activity, pursuing delicious food is human proper life requirement, the history in 5,000 years of Chinese civilization has bred cooking culture of long standing and well established, and spicy good to eat Sichuan cuisine, light fresh and alive Guangdong dishes, antique Anhui cuisine etc. all are the diet that the people is had a very good appetite.In the face of so delicious temptation, perhaps few people can stand fast at go on a diet, the conviction of vegetarian diet.The 3rd, foreign fast food has started the meals culture of " three-hypers one is low " at home.What the Chinese nation taked external cooking culture is a kind of attitude of containing, and along with the enforcement of China's reform policy, the Chinese catering trade is being smiled and met various countries' cuisines to enter China.These several years, local flavor diet all over the world marched China one after another, and its Chinese and western fast food is the fastest at Chinese development, and the west fast food such as " McDonald ", " KFC " that is called foreign fast food by Chinese is accepted by many Chinese.And we know that " characteristics of foreign fast food are three-hypers, three low, promptly higher fatty acid, high protein, high heat, low fiber, lower mineral, low vitamin.Though the existing at present many reports of the harm of foreign fast food, people still keep out incessantly its temptation.So people have entered such one " vicious circle ": on the one hand higher fatty acid, the high protein of absorption without restraint, high caloric diet, satisfying the psychological need of oneself, but do not worry the invasion of affluenza on the other hand all the time!

As seen,, the appealing people grow louder and stronger even if changing the sound of dietary habit, and also of no avail." bread is the staff of life ", we can't stop the pursuit of people to cuisines, also their dietary habit for many years of unable change.Therefore we can't help and ask: a kind of method that makes the best of both worlds is arranged? can glut oneself with delicacies, but can control simultaneously the transformation of taking in energy when can make you face delicious food, reduce fat deposition in vivo, promote the drainage of high salt sodium, potassium, thereby the excessive risk rate of diseases such as obesity, hyperlipidemia, hyperglycemia, hypertension reduce to take place and reach healthy purpose, the dream that eats health that realization can " carefree ".Answer is sure: the culture in 5,000 years of the Chinese nation is of extensive knowledge and profound scholarship, accumulated abundant diet theory, wherein " regimen " is widely known by the people, and its marrow is not to cure the disease, but improve people's physical function by food, the development of blocking-up or reverse sub-health state.

In recent years, the definite and magical effect of the numerous and confused discovery antioxidation that Bordeaux Rouge brought of American-European-Japanese bioscience man, defying age, removing free radical.The Frenchman is the main body diet with smoked oil, chocolate, cheese dessert, western-style food and French last course of a feast, though calorie is very high in these diet, the Frenchman seldom suffers from cardiovascular disease, the famous what is called " mystery in France " in biotechnology field, the world that Here it is.Follow-up study through hundreds of bioscience man decades confirms, this is all relevant with the effect materials such as resveratrol that are rich in the red wine.Frenchman's diet contains high oil, high fat, high calorie; but the ratio of suffering from cardiovascular disease but is minimum in the western developed country; and no matter man, women; can both keep tall and straight well-balanced stature from nonage period to person in middle and old age; its reason just is that the Frenchman likes ardently red wine, no matter men and women, old and young, no matter any occasion all can be drunk several glasss of red wine; the almost every meal of majority must be used, and has just become the cardiovascular maximum person who has rendered outstanding service of Protection Code compatriots for a long time.The high-load Fructus Vitis viniferae extract of indication of the present invention and Chinese various plants and Chinese medicinal herbs extract, through scientific compatibility, and the strictness by tens and the pharmacological testing of science, scientific experiments proves that this combination merged the science elite of eastern and western civilization, the red wine culture of France civilization and the abundant science compatibility of dietetic therapy culture of east Chinese civilization are combined, bring brand-new health care theory and brand-new hope will for the in-problem crowd of human cardiovascular health.

U.S. authority science periodical " nature " in November, 2006 delivered Harvard University's medical college with Fructus Vitis viniferae extract in white mouse experimental result on one's body: when eating high lipid food to white mice, give high-load Fructus Vitis viniferae extract: the quality of life of giving the white mouse of Fructus Vitis viniferae extract significantly is better than the white mouse of simple consumption high lipid food, the survival life-span also obviously prolongs, and motor function obviously improves; Some blood parameters such as cholesterol, triglyceride also significantly improve.Its conclusion highly visible has confirmed the supposition that people are of long duration with strict experiment for the first time in mammal: it is practicable utilizing Fructus Vitis viniferae extract to improve mammiferous health, also is the new method for the treatment of dependency disease such as middle-aged and elderly people hyperlipidemia, hypertension, obesity and disease.High-load Pericarpium Vitis viniferae elite part has very remarkable influence to people's cardiovascular health and life-span! U.S. scientist finds that the elite of Pericarpium Vitis viniferae partly is rich in compositions such as powerful antioxidant-resveratrol and flavonoid, and it has thrombolytic, the anticoagulant benefit of aspirin medicine, can prevent thrombosis! Fructus Vitis viniferae is a kind of very common fruit, and its peel has so effect unexpectedly, the positive strong support of this discovery " dietetic therapy " this theory in my Chinese nation's cooking culture.

In view of the enlightenment of above-mentioned experiment, we find the people enjoy " modern civilization disease " to be perplexed, and that is expected also not a kind ofly just but can effectively play a role under the prerequisite that does not influence the normal diet custom, keep their healthy food?

At present, the health food in market, the supermarket, nutraceutical a feast for the eyes really, the five colours appear.Examine, be not difficult to find that wherein all be in eight or nine cases out of ten is main flow with " replenishing " various vitamin, aminoacid and other human body desired nutritionals element; Be particular about in the family be mend clearly summer, big benefit of winter, all be throughout the year " benefit " word right overhead.This has also embodied a concentrated reflection of the rationale of Chinese medicine dietetic therapy " treatment of deficiency-syndrome by reinforcement ".We think: one " benefit " can not be universally applicable, and one " benefit " can not cover problems.We need create a kind of novel diet science that grows with each passing hour in conjunction with the spirit and needs in epoch! Our theory is exactly: " clearly ", " subtracting ", " falling ", " control ", " disappearing "." clearly " promptly be purify the blood, clear away heart-fire, liver heat removing, lung heat clearing, clearing gallbladder, bowel relieving, remove human body waste; " subtract " promptly under the situation that does not influence diet, reduce absorption high fat, high salt, high heat; " fall " be meant blood fat reducing, bring high blood pressure down, blood sugar lowering, reduce the incidence rate of complication; " control " is meant controlling obesity, controlling body weight; " disappear " the diet worry that refers to eliminate people, mediate their trouble and worry.

My company is in this purpose just provides a kind of like this product, on the basis that makes full use of the modern scientific research achievement, thousands of years " dietetic therapy " elite of long standing and well established of France civilization and Chinese civilization have been merged, the product of integration of edible and medicinal herbs in thousand have been spread in many employings, extract refining its active ingredient through scientific methods, prepared natural plant extracts can be controlled body to taking in the absorption of energy, reduce fat accumulation in vivo, reduce the harm of superfluous nutrition to body, blood fat reducing, purify the blood, blood sugar control, the development of blocking-up and reverse sub-health state can be widely used in food, food additive, application in health food and the medicine.

Summary of the invention:

Technical problem to be solved by this invention is that a kind of natural plant extracts that comprises Pericarpium Vitis viniferae, haw berry, Folium Nelumbinis, Fructus Lycii, Fructus Hippophae, Herb Gynostemmae Pentaphylli, Radix Codonopsis, Radix Polygoni Multiflori Preparata etc. and preparation method thereof will be provided, and the application of this extract in preparation food, food additive, health food, medicine also is provided.

The present invention is by above-mentioned natural plants is made up according to certain consumption, extract through solvent, concentrate a series of modern extraction process flow processs such as drying, extract effective component wherein, the application that is used for preparing food, food additive, health food and medicine.

The amount ranges of the used medical material of extract provided by the present invention is designated as with weight: Pericarpium Vitis viniferae 100-1000 part, haw berry 10-50 part, Folium Nelumbinis 5-30 part, Fructus Lycii 5-30 part, Fructus Hippophae 5-30 part, Herb Gynostemmae Pentaphylli 5-20 part, Radix Codonopsis 5-20 part, Radix Polygoni Multiflori Preparata 1-15 part; Preferred amount ranges is with weight note Pericarpium Vitis viniferae 400-800 part, haw berry 20-40 part, Folium Nelumbinis 10-20 part, Fructus Lycii 10-20 part, Fructus Hippophae 10-30 part, Herb Gynostemmae Pentaphylli 5-15 part, Radix Codonopsis 5-15 part, Radix Polygoni Multiflori Preparata 1-10 part; Optimum amount is with 600 parts of Pericarpium Vitis viniferaes of weight note, 30 parts of haw berries, 15 parts on Folium Nelumbinis, 15 parts of Fructus Lycii, 15 parts of Fructus Hippophaes, 10 parts of Herb Gynostemmae Pentaphylli, 10 parts of Radix Codonopsis, 5 parts of Radix Polygoni Multiflori Preparatas.

Extract provided by the present invention is to be prepared from by following method:

A, independent extraction method:

A), in Fructus Lycii, Folium Nelumbinis, Herb Gynostemmae Pentaphylli, Radix Codonopsis, add 5～15 times of water gagings respectively and decoct 2～3 times, decocted 1～2 hour at every turn, the water extract is concentrated, drying obtains Fructus Lycii extract, Folium Nelumbinis extract, Herb Gynostemmae Pentaphylli extract and Radix Codonopsis extract respectively;

B), in Pericarpium Vitis viniferae, haw berry, Fructus Hippophae, Radix Polygoni Multiflori Preparata, add 5～10 times of amount 65～90% alcohol reflux respectively 2～3 times, the each extraction 1～2 hour, filter, discard medicinal residues, filtrate is concentrated, drying makes Pericarpium Vitis viniferae extract, Fructus Crataegi extract, Fructus Hippophae extract and Radix Polygoni Multiflori Preparata extract respectively;

C), eight kinds of extracts of step a) and step b) gained are mixed, promptly get extract provided by the present invention.

B, mixed extraction method:

A), Fructus Lycii, Folium Nelumbinis, Herb Gynostemmae Pentaphylli, Radix Codonopsis are mixed, adds 5～15 times of water gagings and decoct 2～3 times, decocted 1～2 hour at every turn, the water extract is concentrated, and drying obtains extract A;

B), Pericarpium Vitis viniferae, haw berry, Fructus Hippophae, Radix Polygoni Multiflori Preparata are mixed, adds 5～10 times of amount 65～90% alcohol reflux 2～3 times, extracted 1～2 hour at every turn, filter, discard medicinal residues, filtrate is concentrated, and drying makes extract B;

C), two kinds of extracts of A, the B of step a) and step b) gained are mixed, promptly get extract provided by the present invention.

In above-mentioned independent extraction method, preparation technology is preferred:

A), adding 10 times of water gagings respectively in a certain amount of Fructus Lycii, Folium Nelumbinis, Herb Gynostemmae Pentaphylli, Radix Codonopsis decocts 2～3 times, the each decoction 1～2 hour, the water extract is concentrated, and drying obtains Fructus Lycii extract, Folium Nelumbinis extract, Herb Gynostemmae Pentaphylli extract and Radix Codonopsis extract respectively;

B), in a certain amount of Pericarpium Vitis viniferae, haw berry, Fructus Hippophae, Radix Polygoni Multiflori Preparata, add 10 times of amount 70～80% alcohol reflux respectively 2～3 times, the each extraction 1～2 hour, filter, discard medicinal residues, filtrate is concentrated, drying makes Pericarpium Vitis viniferae extract, Fructus Crataegi extract, Fructus Hippophae extract, Radix Polygoni Multiflori Preparata extract respectively;

C), eight kinds of extracts of step a) and step b) gained are mixed, promptly get extract provided by the present invention.

In above-mentioned mixed extraction method, preparation technology is preferred:

A), a certain amount of Fructus Lycii, Folium Nelumbinis, Herb Gynostemmae Pentaphylli, Radix Codonopsis are mixed, adds 10 times of water gagings and decoct 2～3 times, decocted 1～2 hour at every turn, the water extract is concentrated, and drying obtains extract A;

B), a certain amount of Pericarpium Vitis viniferae, haw berry, Fructus Hippophae, Radix Polygoni Multiflori Preparata are mixed, adds 10 times of amount 70～80% alcohol reflux 2～3 times, extracted 1～2 hour at every turn, filter, discard medicinal residues, filtrate is concentrated, and drying makes extract B;

C), two kinds of extracts of A, the B of step a) and step b) gained are mixed, promptly get extract provided by the present invention.

Natural plant extracts provided by the present invention can be used for the preparation of food, food additive, health food, also can form as prescription, through the modern pharmaceutical technology, carries out deep processing, is prepared into medicine.Mainly provide the application of the preparation that is used for food, health food, food additive in the present invention.

Natural plant extracts provided by the present invention can effectively control body to the absorption of taking in energy, reduce in vivo accumulation of fat, reduce the harm of superfluous nutrition body, blood fat reducing, purify the blood, blood sugar control, controlling body weight is removed rubbish in the body, the development of blocking-up and reverse sub-health state, can reduce the onset risk of hyperlipidemia, hypertension, diabetes, coronary heart disease, obesity, fatty liver diseases, can be edible for healthy population, keep fit; Also can be edible for sub-health population, effectively block and reverse the development of sub-health state, can also pass through deep processing, be used for preparing the application of the medicine of diseases such as treating hypertension, hyperlipemia, diabetes.

Its concrete effect explaination is as follows:

(1) regulates lipid metabolism, reduce the incidence rate of cardiovascular disease.Reduce the content of cholesterol in serum, triglyceride, reduce low density lipoprotein, LDL, high density lipoprotein increasing, effectively regulate lipid metabolism, brought into play the effect of blood " street cleaner ", thereby can reduce the deposition of lipid, reduce the formation of arteriosclerosis plaque, reduce the risk of coronary artery, cerebral atherosclerosis at ductus arteriosus wall.

(2) bring high blood pressure down, blood viscosity lowering suppresses biologically active pdgf, suppresses thrombosis, reduces disease incidence rate such as hypertension, myocardial infarction, cerebral thrombosis.

(3) improve hemorheological property, improve the body microcirculation, effectively prevent acute cerebral insult, and can also produce repair nerve injury.

(4) antioxidation is effectively removed free radical, and protection blood vessel endothelium system avoids the infringement of free radical, protection vascular health; Effectively to the reperfusion injury of antioxidant radical after, thereby effectively protect heart, reduce infarction size myocardial ischemia.

(5) blood sugar regulation, the sickness rate of reduction diabetes, the caused complication of prevent diabetes.Effectively improve the microcirculation of β cell; increase the blood oxygen metabolism of islet cells; eliminate the infiltration of interleukin to islet cells; suppress the generation of oxygen-derived free radicals; repair; activate beta Cell of islet that damage or atrophy; the biological function of protection glucose tolerance factor; regulate the affinity of Insulin receptor INSR; quicken the acceptance and the running of islets of langerhans receptor, strengthen the sensitivity of insulin target cell, reduce the synthetic of glycogen; the heteroplasia that suppresses glycogen; make secretion of insulin drain running and reach perfect condition, thereby repair patient's islet function, effectively prevent and treat the generation and the development of complication.

(6) the liver protecting, the prevention fatty liver.Because can effectively regulate lipid metabolism, thereby can reduce the deposition of fat at liver, the liver protecting reduces the probability that fatty liver takes place; Hepatic tissue to damage has the excellent repairing effect, the function of detoxification of energy enhance hepatocyte.

(7) generation of minimizing obesity.This product can suppress the absorption of superabundant fats, reduces the increase of body quality, reduces the obesity odds.

(8) facilitating digestion improves the function of gastric gland secretion and Digestive system, improves gastrointestinal peristalsis, regulates the function of intestinal canal disorder, repairs gastric mucosa, repairs deep layer ulcer, promotes the intestinal mucosa Regeneration and Repair, keeps intestinal eubiosis.

(9) regulate body's immunological function, the cellular immunization and the humoral immunization of body all had remarkable influence.

(10) auxiliary improvement of memory.

(11) resisting fatigue, hypnotic improves the quality of sleeping.

(12) radioprotective, anoxia enduring.

(13) loosening bowel to relieve constipation is discharged the body toxin, enhances metabolism the occurrence probability of intestinal tract diseases such as reduction rectal cancer.

(14) the energy nourishes rough skin promotes cellular metabolism, promotes epithelial tissue regeneration, has antiallergic, antibacterial, strong penetration and protection skin natural colour, keeps effects such as youth, slow down aging.

Natural plant extracts provided by the present invention can be formed the preparation that is used for food, food additive, health food and medicine as prescription or main prescription through any feasible mode, its crowd who is suitable for is very extensive, can be healthy population, sub-health state crowd (potential ill crowd), also can be ill crowd; Can be used for middle-aged and elderly people, also can be used for adolescence, be particularly suitable for big, the unusual to some extent people of blood fat/blood pressure/blood glucose of those operating pressures.Except suitable hyperlipidemia, hypertension, hyperglycemia " three-hypers disease " crowd took, also suitable those coronary heart disease, angina pectoris, arteriosclerosis, fatty liver, obesity, immunologic hypofunction person ate.The natural plant extracts that is provided can effectively control body in vivo the accumulation of the absorption of taking in energy and conversion, minimizing fat, reduce the deposition of fat at liver, reduce the harm of superfluous nutrition to body, blood fat reducing, purify the blood, blood sugar control, controlling body weight is removed rubbish in the body, the continuation of blocking-up and reverse sub-health state develops, and can reduce the onset risk of hyperlipidemia, hypertension, diabetes, coronary heart disease, obesity, fatty liver diseases.Food with this natural plant extracts preparation can make healthy population continue the state of keeping fit, avoid the infringement of " three-hypers disease ", also can block and reverse sub-health state, reduce or delay the transformation of sub-health state crowd to ill crowd to " three-hypers disease " development.Prescription provided by the present invention and natural plant extracts thereof can also pass through certain processing, are prepared into various health product and eat for healthy, sub-health population, bring into play its distinctive effect; Also can pass through deep processing, be used for preparing the application of the medicine of diseases such as treatment hypertension, hyperlipemia, diabetes.

Below further explain the present invention with embodiment, but the present invention is not limited to this.

Specific embodiment:

1, preparation method of extract:

A), Fructus Lycii, Folium Nelumbinis, Herb Gynostemmae Pentaphylli, Radix Codonopsis added 10 times of water gagings respectively decoct 3 times, decocted 1 hour at every turn, the water extract is concentrated, drying obtains Fructus Lycii extract, Folium Nelumbinis extract, Herb Gynostemmae Pentaphylli extract and Radix Codonopsis extract respectively;

B), Pericarpium Vitis viniferae, haw berry, Fructus Hippophae, Radix Polygoni Multiflori Preparata add 8 times of amount 70% alcohol reflux respectively 3 times, extracted 1 hour at every turn, filter, discard medicinal residues, filtrate is concentrated, and drying makes Pericarpium Vitis viniferae extract, Fructus Crataegi extract, Fructus Hippophae extract and Radix Polygoni Multiflori Preparata extract respectively;

C), eight kinds of extracts of step a) and step b) gained are mixed, promptly get extract provided by the present invention.

2, preparation method of extract

A), in Fructus Lycii, Folium Nelumbinis, Herb Gynostemmae Pentaphylli, Radix Codonopsis, add 10 times of water gagings and decoct 3 times, decocted 1 hour at every turn, the water extract is concentrated, drying obtains extract A;

B), in Pericarpium Vitis viniferae, haw berry, Fructus Hippophae, Radix Polygoni Multiflori Preparata, add 10 times of amount 70% alcohol reflux 3 times, extracted 1 hour at every turn, filter, discard medicinal residues, filtrate is concentrated, drying makes extract B;

C), two kinds of extracts of A, the B of step a) and step b) gained are mixed, promptly get extract provided by the present invention.

3, the application of extract

1), food:

Adding 8 times of water gagings in 15kg Fructus Lycii, 15kg Folium Nelumbinis, 10kg Herb Gynostemmae Pentaphylli, 10kg Radix Codonopsis respectively decocts 3 times, the each decoction 2 hours, the water extract is concentrated, and drying obtains Fructus Lycii extract, Folium Nelumbinis extract, Herb Gynostemmae Pentaphylli extract and Radix Codonopsis extract respectively; In 600kg Pericarpium Vitis viniferae, 30kg haw berry, 15kg Fructus Hippophae, 5kg Radix Polygoni Multiflori Preparata, add 10 times of amount 65% alcohol reflux respectively 3 times, the each extraction 1 hour, filter, discard medicinal residues, filtrate is concentrated, drying makes Pericarpium Vitis viniferae extract, Fructus Crataegi extract, Fructus Hippophae extract and Radix Polygoni Multiflori Preparata extract respectively; Eight kinds of extracts of gained are mixed drying.

Above-mentioned natural plant extracts adds edible adjuvant, becomes all acceptable food forms such as tablet, capsule, granule, oral liquid according to the prepared of routine.

2) food additive:

Add 12 times of water gagings and decoct 3 times in Fructus Lycii, Folium Nelumbinis, Herb Gynostemmae Pentaphylli, Radix Codonopsis, decocted 1 hour at every turn, the water extract is concentrated, drying obtains extract A; Add 10 times of amount 75% alcohol reflux 3 times in Pericarpium Vitis viniferae, haw berry, Fructus Hippophae, Radix Polygoni Multiflori Preparata, extracted 1 hour at every turn, filter, discard medicinal residues, filtrate is concentrated, drying makes extract B; Two kinds of extracts of A, B of gained are mixed, promptly get extract provided by the present invention.

Above-mentioned natural plant extracts is added in the various edible carriers such as noodles, flour, bread, rice flour, Amylum Nelumbinis Rhizomatis, lotus powder, cookies, beverage, be prepared into various types of food or beverage.

3) health food:

Add 10 times of water gagings and decoct 2 times in 15kg Fructus Lycii, 15kg Folium Nelumbinis, 10kg Herb Gynostemmae Pentaphylli, 10kg Radix Codonopsis, decocted 2 hours at every turn, the water extract is concentrated, drying obtains extract A; Add 10 times of amount 70% alcohol reflux 3 times in 600kg Pericarpium Vitis viniferae, 30kg haw berry, 15kg Fructus Hippophae, 5kg Radix Polygoni Multiflori Preparata, extracted 1 hour at every turn, filter, discard medicinal residues, filtrate is concentrated, drying makes extract B; Two kinds of extracts of A, B of gained are mixed, promptly.

Above-mentioned natural plant extracts is added adjuvant, according to the preparation method of routine, be prepared into the health food of all dosage forms of acceptable on the pharmaceutics, for example tablet, capsule, oral liquid, electuary etc.

4) medicine:

Add 15 times of water gagings respectively and decoct 3 times in Fructus Lycii, Folium Nelumbinis, Herb Gynostemmae Pentaphylli, Radix Codonopsis, decocted 1 hour at every turn, the water extract is concentrated, drying obtains Fructus Lycii extract, Folium Nelumbinis extract, Herb Gynostemmae Pentaphylli extract and Radix Codonopsis extract respectively; In Pericarpium Vitis viniferae, haw berry, Fructus Hippophae, Radix Polygoni Multiflori Preparata, add 8 times of amount 85% alcohol reflux respectively 3 times, extracted 1 hour at every turn, filter, discard medicinal residues, filtrate is concentrated, and drying makes Pericarpium Vitis viniferae extract, Fructus Crataegi extract, Fructus Hippophae extract, Radix Polygoni Multiflori Preparata extract respectively; Eight kinds of extracts of gained are mixed, drying, promptly.

Above-mentioned natural plant extracts is through deep processing, the middle pharmaceutic adjuvant that adds, be prepared into any dosage form of acceptable on the pharmaceutics according to conventional method, can be conventional formulation forms such as tablet, granule, hard capsule, soft capsule, pill, oral liquid, injection, also can be prepared into special preparations such as slow releasing agent, controlled release agent, dispersant, effervescent.

Below further explain the present invention by functional experiment.

One, the influence that experimental hyperlipidemia rabbit blood fat and atherosclerosis are formed:

Experimental technique:

Get normal feedstuff and stablize the rabbit in 2 weeks.Be divided into 3 groups (model group, extract I group, extract II groups) at random by body weight, every treated animal is irritated stomach and is given cholesterol 0.2g/kg when giving normal feedstuff, once-a-day, the administration group is administered once every day, and model group gives normal saline, continuous 8 weeks, 2,4,6,8 weeks were got blood by ear vein after modeling, and 3000rpm is centrifugal, and separation of serum is equipped with to be surveyed, sacrifice of animal after 8 weeks, take out aorta by the side longitudinal incision, with 10% formalin fixed 24 hours, with soudan III dyeing 30min.Carry out the classification of aorta speckle and the measuring and calculating of plaque area.Experimental result is represented with x ± sd.

Table 1: this product is to the influence of high fat rabbit anteserum triglyceride

Annotate: and model group is relatively ^*P＜0.05, ^*P＜0.01; Compare with normal value ^##P＜0.01

Table 2: this product is to the influence of high fat rabbit anteserum T-CHOL

Annotate: and model group is relatively ^*P＜0.05, ^*P＜0.01; Compare with normal value ^##P＜0.01

Table 3: this product is to the atherosclerotic influence of high fat rabbit

Annotate: and model group is relatively, ^*P＜0.01

Experimental result:

This product successive administration can reduce hyperlipemia rabbit anteserum cholesterol and triglyceride levels, significantly suppresses atherosclerotic generation.Prompting this product has the effect of regulating lipid metabolism, prevention and the diabetes complicated abnormalities of sugar/lipid metabolism of treatment.

Two, to the preventive effect of alloxan induced hyperglycemia mice:

Experimental technique:

Get 30 of male Kunming kind white mice, be divided into 3 groups (extract I group, extract II group, blank groups) at random, 10 every group, administration group prevention gastric infusion is 30 days before the experiment, and the blank group is given distilled water.After the last administration 2 hours, tail vein injection alloxan 60mg/kg got blood and surveys blood glucose after 72 hours.Contrast between the work group.

Table 4: this product is to the influence (representing with x ± sd) of hyperglycemia mouse blood sugar level.

Annotate: and the normal saline group is relatively ^*P＜0.01

Experimental result:

This product prevention administration can prevent the generation of alloxan induced mice hyperglycemia.

Three, to the hypertensive influence of rat experiment (acute renal artery stenotic type)

Experimental technique:

Get the healthy SD rat, ♂, 250-300g is divided into administration I group, administration II group and matched group at random, and successive administration is 30 days after weighing, and matched group is given normal saline.Pentobarbital sodium with 0.3% during experiment is anaesthetized 1ml/100g, prostrate being fixed in deducts breast, lower back hair on the rat operating-table, cut skin to third lumbar vertebra along median line from the 10th thoracic vertebra, 1.5-2cm and under the rib of left side apart from vertebra 1cm place, separate left kidney, with kidney the base of a fruit with little metal clip clamping 4-5 hour.Separate trachea, insert tracheal intubation, ligation.Separate carotid artery, the plastic tube that will contain 0.1% heparin-saline inserts common carotid artery, and ligation is fixed.Another termination blood pressure transducer inserts four roads connection, recording blood pressure.Decontrol the little metal clip of kidney the base of a fruit, rat blood pressure is increased to plateau value, recording blood pressure gradually.

Table 5:

Annotate: and matched group is relatively ^*P＜0.05, ^*P＜0.01

Experimental result: this product has the effect that brings high blood pressure down.

Four, the ligation coronary artery is caused the influence of Acute Myocardial Ischemia in Rats

Experimental technique:

Get 40 of SD rats, male and female half and half, body weight 250-300g is divided into 4 groups at random, that is: sham operated rats, model group, 1 group of extract, 2 groups of extracts.Each treated animal difference gastric infusion (10ml/kg, sham operated rats and model group give the distilled water of equal volume) of elder generation is 7 days before the experiment, behind the last administration 1h, lumbar injection urethane 1g/kg anesthesia, get dorsal position and fix, it is subcutaneous that needle electrode carefully thrusts extremity, measures the II lead electrocardiogram.Separate trachea, intubate connect artificial respirator (tidal volume 30ml, respiratory frequency 60 times/min), open breast in breastbone left side 3-4 intercostal is then opened pericardium, left anterior descending coronary artery place threading between arterial cone and left auricle overlaps standby with plastic tube.Postoperative is stablized 20min, and electrocardiogram before the record ligation merges plastic tube ligation 60min, and the electrocardiogram of record 30min, 60min.Then take out plastic tube and irritate 90min again, and the electrocardiogram of record 45min, 90min.And in experiment end back abdominal aortic blood, the centrifugal 15min of 3000r/min, preparation serum, automatic clinical chemistry analyzer are surveyed CK, the LDH value is carried out statistical test.

Table 6: this product is to influence (X ± S) (n=10) of rats with myocardial ischemia ECG T wave due to the coronary ligation

Annotate: compare with model group: ^*P＜0.05, ^*P＜0.01; Compare with sham operated rats: ^#P＜0.05, ^##P＜0.01.

The result shows:

Model group when coronary ligation 30min, 60min the T ripple than sham operated rats obviously raise (P all＜0.01); The multiple back T ripple of irritating obviously reduces, but still is higher than sham operated rats (P＜0.05); The modeling success is described.The T ripple raise when 1 group of extract can resist ligation 30min, 60min, with model group notable difference (P＜0.05) was arranged relatively; The T ripple raise when 1,2 groups of extracts can resist ligation 30min, 60min, with remarkable (P＜0.05 of model group comparing difference; P＜0.01); Multiple 1, the 2 groups of T ripples of back extract of irritating all reduce, and 2 groups of extracts compare with model group at the multiple back 45min that irritates that there were significant differences (P＜0.05).Prompting this product has the certain protection effect to the myocardial ischemia due to the coronary ligation, and presents certain dose-effect relationship.

Table 7: this product is to influence (X ± S) (n=10) of rats with myocardial ischemia serum CK, LDH value due to the coronary ligation

Annotate: compare with model group: * P＜0.05, * * P＜0.01; Compare with sham operated rats: ^##P＜0.01.

The result shows:

1,2 groups of CK values of extract reduce, with remarkable (P＜0.05 of model group comparing difference; P＜0.01); 2 groups of LDH values of extract reduce, with model group comparing difference remarkable (P＜0.05).Prompting this product can suppress myocardial ischemia/again irritates the myocardial cell that causes and discharges CK, LDH, myocardial cell is had the certain protection effect, and present certain dose-effect relationship.

Five, to the protective effect of Acute Myocardial Ischemia in Rats

Experimental technique:

Select 40 of body weight 250g left and right sides male rats for use, be divided into 3 groups at random, 10 every group.Gastric infusion 7 days, 1 hour rat that will regain consciousness lies on the back fixing after the last administration, the normal ECG of record precordial leads, quiet then notes pituitrin 1.5u/kg, injection finishes in the 5s, injection back 15s, 30s, 45s, 1min,, 2min and 5min respectively write down electrocardiogram one time, with give in the pituitrin 30s S-T section raise more than the 1mm or in the 2min T ripple descend 50% or more or inversion as the index of myocardial ischemia, represent the myocardial ischemia negative rate the myocardial ischemia number of animals not occur with the ratio of used number of animals.Experimental data is carried out statistical procedures with the t check system.

Table 8:

Annotate: and matched group is relatively ^*P＜0.01

Experimental result: as can be seen from the above table, this product has significant protective effect to the myocardial ischemia due to the pituitrin.

Six, to the hemodynamic influence of anesthetized dog

Experimental technique:

Get domesticated dog, the male and female dual-purpose is divided into 3 groups of (1) sham operated rats: give the isometric(al) normal saline at random; (2) extract I group; (3) extract II group.The used medicine of each treated animal is made into suitable concn with suitable solvent, and the administration volume is 2ml/kg.With the subcutaneous cephalic vein anesthesia of 3% pentobarbital sodium 30mg/kg forelimb, back of the body position is fixed on the operating-table, cuts off the hair of cervical region, chest and left hind inboard.Separate trachea and insert tracheal intubation; Separate femoral vein and insert venous cannulation, slowly constant speed input normal saline (about 1ml/min); Separate femoral artery and insert arterial cannulation (being full of the heparin-saline of 500u/ml in the pipe), to measure arteriotony.Under the artificial respiration, open breast, cut off pericardium in the 4th intercostal, be sewn in thoracic wall, separate root of ascending aorta and ramus descendens anterior arteriae coronariae sinistrae, place the electromagnetic blood flowmeter probe (12mm of suitable internal diameter, 2mm), be connected in and measure cardiac output (CO) and coronary flow (CBF) on the electromagnetic blood flowmeter.Left ventricular cannulation (being full of heparin-saline in the pipe) in left ventricle apex wound inserts left ventricle, is measured left indoor pressure (LVSP), left chamber diastasis pressure (LVEDP), the maximum climbing speed (LVdp/dtmax) of intraventricular pressure; It is subcutaneous that needle electrode is inserted the dog extremity, recording ecg (ECG).After waiting to stablize, separate duodenal administration, directly write down mean arterial pressure (MAP), heart rate (HR), cardiac output (CO), coronary flow (CBF), measure and calculate MAP, HR, CO, CBF, LVSP, LVEDP, ejection time, SV (stroke volume), CI (cardiac index), SI (SI), LVWI (stroke work index), TTI (total oxygen consumption index), the TPVR (peripheral vascular resistance always) of each time period anesthetized dog of administration front and back.

Computing formula:

CI (cardiac index)=CO/ body surface area SV (stroke volume)=CO*1000/HR

SI (SI)=CI*1000/HR TTI (total oxygen consumption index)=MAP*HR* ejection time

TPVR (total peripheral vascular resistance)=MAP/CO

LVWI (stroke work index)=CI*1.025* (SAP-0.667) * 13.6*0.001

Table 9 this product is to influence (X ± S) (n=5) of anesthetized dog mean arterial pressure (MAP)

Table 10 this product is to influence (X ± S) (n=5) of anesthetized dog heart rate (HR)

Table 11 this product is to influence (X ± S) (n=5) of anesthetized dog cardiac output (CO)

Compare with sham operated rats ^*P＜0.05

Table 12 this product is to influence (X ± S) (n=5) of anesthetized dog coronary flow (CBF)

Compare with sham operated rats ^*P＜0.05

Table 13 this product is to influence (X ± S) (n=5) of anesthetized dog stroke volume (SV)

Compare with sham operated rats ^*P＜0.05, ^*P＜0.01

Table 14 this product is to influence (X ± S) (n=5) of anesthetized dog cardiac index (CI)

Compare with sham operated rats ^*P＜0.05

Table 15 this product is to influence (X ± S) (n=5) of anesthetized dog SI (SI)

Compare with sham operated rats ^*P＜0.05, ^*P＜0.01

Table 16 this product is to influence (X ± S) (n=5) of anesthetized dog ejection time

Compare with sham operated rats, ^*P＜0.05, ^*P＜0.01

Table 17 this product is influence (X ± S) (n=5) of work index (LVWI) to chamber, an anesthetized dog left side

Table 18 this product is to influence (X ± S) (n=5) of the total oxygen consumption of anesthetized dog (TTI)

Compare with sham operated rats ^*P＜0.05, ^*P＜0.01

Table 19 this product is to influence (X ± S) (n=5) of anesthetized dog blood vessel total peripheral resistance (TPVR)

Compare with sham operated rats ^*P＜0.05, ^*P＜0.01

The result shows:

This product extract II group can increase anesthetized dog cardiac output (CO), dog cardiac index (CI), obviously increase stroke volume (SV) and dog SI (SI), two dosage groups of extract I and extract II can increase anesthetized dog coronary flow (CBF), reduce total oxygen consumption of anesthetized dog (TTI) and blood vessel total peripheral resistance (TPVR).The prompting this product tool hemodynamics that has some improvement can reduce afterload, reduce the effect of cardiac work, cardiac function enhancing.

Seven, to the influence of platelet aggregation

Get 30 of kunming mices, the male and female dual-purpose, mice is divided into 3 groups at random, successive administration 7 days (matched group is given normal saline), every day, gastric infusion was 1 time.30min after the last administration, get anticoagulation, centrifugalize goes out platelet blood plasma 0.1ml, places on the silicidation microscope slide, put into 1 of glass bead unshakable in one's determination in the blood plasma, microscope slide is placed on the heating magnetic stirring apparatus, and 37 ℃ of preheating 2min start agitator, change 5min with 1000r/min, take off microscope slide, first perusal platelet aggregation number, rearmounted microscopically is observed the platelet aggregation number.

Table 20: this product is to the influence of mouse platelets aggregation capability

Group	Number of animals (n)	Dosage (g/kg)	The platelet aggregation number
				Extract I group extract II group matched group	10 10 10	6 3	0.90±0.41 ** 0.93±0.94 * 1.40±0.38

Annotate: and matched group is relatively ^*P＜0.01; ^*P＜0.01.

Experimental result shows:

This product and matched group compare, and the platelet aggregation ability obviously reduces, and illustrates that this product can suppress hematoblastic gathering.

Eight, to the hemorheological influence of stasis syndrome rat model:

Experimental technique:

Get with the Wistar rat of the body weight under the raising condition about 300g, male and female half and half are divided into 4 groups (blank group, model group, extract I group, extract II groups), one week of administration group successive administration at random, once a day, model group and blank group all give isometric normal saline.After last administration in the 7th day, except that the blank group, equal adrenaline subcutaneous injection 0.8ml/Kg, totally twice, 4 hours at interval, between biphasic injection Adr, rat is immersed 5` in the frozen water, stop eating after the disposal, get blood examination inferior morning and survey.

Table 21:

Annotate: and model group is relatively ^*P＜0.05, ^*P＜0.01; Compare with the blank group ^##P＜0.05

Table 22:

Annotate: and model group is relatively ^*P＜0.05, ^*P＜0.01; Compare with the blank group ^##P＜0.05

Experimental result:

Can significantly reduce the level of whole blood contrast viscosity, serum specific viscosity with model group comparison this product, improve RBC electrophoresis index, reduce the RBC hematocrit, prompting this product has the effect that improves hemorheological property.

Nine, the effect of antagonism rat suppository formation:

Experimental technique:

Get the healthy SD rat, ♂, 200-300g is divided into extract I group, extract II group and matched group at random, and successive administration is 7 days after weighing, and matched group is given normal saline.Weigh during experiment, lumbar injection pentobarbital sodium 0.05g/Kg, insert polyethylene tube in the trachea so that remove the trachea endocrine, and separation right common carotid artery and left external jugular vein, put into four long trumpeter's art silk threads of a 5cm in three sections polyethylene tube stage casings, be full of heparin-saline solution (50u/ml) two ends in the polyethylene tube and insert right common carotid artery and left external jugular vein respectively.The recovery blood flow is middle Herba Clinopodii after 15 minutes, takes out silk thread rapidly and weighs, and it is wet weight of thrombus that gross weight deducts silk thread weight, calculates suppression ratio.

Table 23:

Experimental result:

Extract of the present invention can effectively suppress rats in vitro thrombosis.(compare with matched group ^*P＜0.01)

Ten, the fat experiment of prevention rat:

Experimental technique:

Get the healthy SD rat, ♂ about 150g, is divided into totally four groups of blank group, model control group, extract I group, extract II groups at random after weighing, model control group and administration group give the nutrient fodder of equivalent every day, and the blank group gives the equivalent normal feedstuff; Experimental group gives the given the test agent of various dose simultaneously, and matched group is given coordinative solvent, continuously 30d.Every animal of experimental session record give appetite, surplus appetite and berley amount, claim body weight weekly twice, weigh when experiment finishes, cut open the belly and get body fat (testis and perirenal fat pad) and weigh, calculate fat/body ratio.

Table 24

Annotate: compare with model group ^*P＜0.01, ^*P＜0.05.

11, the effect (pylorus ligature law) of the experimental gastric ulcer of Chinese People's Anti-Japanese Military and Political College Mus:

Experimental technique:

Get the rat of body weight about 180g, be divided into 3 groups (matched group, extract I group, extract II groups) at random, every group of equal successive administration is after 30 days (matched group is given normal saline), and fasting is after 48 hours, the capable pylorus ligation operation of etherization, stop to supply water, put to death rat after 18 hours, get stomach and cut off coat of the stomach along greater gastric curvature for food, clean content, coat of the stomach is launched to fix, observe the gastric mucosa face, write down ulcerogenic Mus number, ulcer number, ulcer level, ulcer index and pathological changes situation.The result represents with x ± sd.

Table 25:

Annotate: compare with matched group ^*P＜0.01

Experimental result: this product can significantly suppress the incidence rate of pylorus ligation mouse gastric ulcer, alleviates ulcer level, reduces ulcer index.

12, ethanol is caused the protective effect of acute mouse liver injury model

Experimental technique:

Get healthy KM mice, ♂, 18-22g is divided into totally four groups of blank group, model group, administration I group, administration II groups at random after weighing, blank group and model group are irritated stomach and are given distilled water, experimental group gives the given the test agent of various dose, 30d altogether once a day, give given the test agent for the last time after, model group and each given the test agent group are once irritated stomach give 50% ethanol 12mL/kg, the blank group is given distilled water, and fasting was put to death animal in 16 hours, carried out every index and pathological examination.

Table 26

Annotate: compare with model group ^*P＜0.01, ^*P＜0.05.

When hepatic tissue suffered damage or be downright bad, ALT, AST promptly overflowed in the cell and advance the human bloodstream, thereby cause serum alt, and AST is active obviously to raise.Therefore, serum alt, the active rising of AST has reflected the degree of hepatocyte injury to a certain extent.This experimental result shows, gives this product in advance and can suppress ethanol and cause mice serum ALT, the active rising of AST.

Ethanol all can produce high malicious product in the intrahepatic metabolism approach: acetaldehyde.Acetaldehyde promotes lipid peroxidation by the interaction with cysteine, GSH and vitamin E.GSH can eliminate the toxicity oxygen-derived free radicals, and the protection cell is not subjected to the electrophilic material damage.Short-term is taken the photograph people's ethanol, and GSH is synthetic to make liver lose GSH in a large number by suppressing.GSH reduces in the liver, and its oxidation resistance is descended, thereby helps the lipid peroxidation due to other factors, causes hepar damnification.This experimental result shows, gives this product in advance and can suppress hepatic tissue MDA rising and the GSH reduction that ethanol causes.As seen, this product stops the exhaustion of the liver GSH due to the ethanol, eliminates excessive harmful free radical in the body, and antagonism ethanol causes hepatocyte injury.

13, mouse spleen lymphocyte transformation experiment

Experimental technique:

Select 18-22g C57BL/6J mice for use, be divided into totally three groups of blank group, extract I group and extract II groups after weighing at random, experimental group is given the various dose given the test agent, the blank group is given distilled water, be total to 30d once a day, administration finishes the aseptic spleen of getting in back, places to fill an amount of aseptic Hank ' s liquid plate, makes the individual cells suspension.In the complete culture solution of 1mL, with the blue dyeing counting viable count of platform phenol, adjusting cell concentration is 3*106/mL with cell suspension.Divide two holes to add in 24 well culture plates cell suspension, every hole 1mL, a hole adds 75 μ l ConA liquid, and 5%CO2 is put in contrast in another hole, cultivates 72h in 37 ℃ of incubators.Cultivate and finish preceding 4h, supernatant 0.7mL is inhaled in every hole gently, adds 0.7mL and does not contain calf serum RPMI1640 culture fluid, adds MTT (5mg/mL) 50mL/ hole simultaneously, continues to cultivate 4h.After cultivating end, every hole adds 1mL acid isopropyl alcohol, and purple crystal is dissolved fully.Divide then to install in 96 well culture plates, each packing 3-6 hole, hole is measured 570nm absorbance (A as parallel sample with microplate reader ₅₇₀).Use A ₅₇₀ConA ⁺-A ₅₇₀ConA ^-Represent lymphocytic multiplication capacity.

Table 27

Experimental result:

This product can promote the inductive mouse lymphocyte multiplication capacity of ConA, illustrates that its non-specific cell immunologic function to body has obvious enhancement effect.

14, the normal mouse peritoneal macrophage is engulfed the influence of digestion chicken red blood cell ability

The experiment grouping:

40 of Kunming mouses are divided into 4 experimental grouies at random, 10 every group; Administration: the normal control group gives starch solution 10g/kg/ days, irritates stomach, continuous 30 days; The administration group is respectively to give this product solution in 6g/kg/ days, 3g/kg/ days, 1.5g/kg/ days.Irritated stomach 30 days;

Experimental technique: the blood serum induced haemolysis rope of sheep red blood cell (SRBC) is surveyed method.

Table 28:

Annotate: and matched group is relatively ^*P＜0.01.

Experimental result:

Mice is taken 30 days testing results of this product and shows that big or middle dosage group mice engulfs digestion chicken red blood cell ability than the control group mice peritoneal macrophage and obviously strengthens, and being reflected at phagocytic index and phagocytic percentage variance statistics has utmost point significant difference; Small dose group mice and matched group compare, and difference is not obvious, shows that phagocytic index and phagocytic percentage variance statistical discrepancy are not obvious.This product that suitable dosage is taken in this explanation can significantly improve the cellular immune function of mice.

15, old rats antioxidation experiment

Experimental technique:

Select 20 monthly age male SD rats for use, be divided into aged matched group, extract I group, extract II group at random, other establishes one 3 months old rats for lacking the matched group in age, age, matched group was all given distilled water to aged matched group with less, experimental group is given the given the test agent of various dose, be total to 30d once a day, experiment finishes to put to death animal, gets blood and surveys MDA, SOD, GSH-Ps.

Table 29

Annotate: compare with aged matched group ^*P＜0.01, ^*P＜0.05.

Experimental result:

This product can effectively reduce the intravital level of lipid of aged Mus and prevent that the interior antioxidase of body is subjected to the oxidative damage of free yl induction, recovers the activity of antioxidase.

16, to the influence of life span of drosophila melanogaster:

Experimental technique: the preparation of fruit bat culture medium: Semen Maydis powder 10g is poured in the 250ml beaker, add tap water 36ml, heating and furnishing pasty state.Claim again 1.5g agar to put in the 100ml beaker and add tap water 40ml, boil and make abundant dissolving that add white sugar 13.5g again, benzoic acid 15g (with 3ml 95% dissolve with ethanol) boils dissolving.Agar and cornmeal mush that dissolving is good mix, and boil, weigh, and add dried yeast powder 1.0g, and 100 ℃ of hot water are fully mixed well to gross weight 100g.Pour into while hot through 160 ℃ and do in the roasting disinfectant culture vessel.The preparation of pastille culture medium: this extract is added appropriate solvent be made into and add above-mentioned culture medium behind the variable concentrations and fully mix well and get final product.Get the female male drosophila of hatching in the 24h, be divided into 4 groups at random, every group of male and female half and half are put into the 3.0cm * 10.0cm glass tubing that fills the thick former culture medium of 2cm and are cultivated.Test tube keeps flat, and when being cultured to 29 days, is divided into 4 groups again at random, put into former culture medium for one group and compare, its excess-three group is put into the glass tubing of pastille culture medium and is cultivated, and regularly changes fresh culture, add up dead fruit bat number every day simultaneously, until whole natural deaths.From the 29th day as the 1st day, calculate existence natural law, average life and maximum life span of each group.

Table 30:

Annotate: compare with matched group: ^*P＜0.01; ^*P＜0.05.

17, to the acquired amnemonic influence of scopolamine induced mice (darkness avoidance test):

Experimental technique:

Get inbred line male mice number, be divided into totally four groups of blank group, model group, extract I group, extract II groups at random.Experimental group gives the given the test agent of various dose, blank group and model group give distilled water, are total to 30d once a day, and each group is respectively at after the last administration, 20min lumbar injection scopolamine before the training, each organizes mice energising training once, experimentizes the incubation period of record mice behind the 24h, after finishing, experiment puts to death immediately, get cerebral tissue,, measure the content of acetylcholine esterase, monoamine oxidase, MAO with NS (1: 9) homogenate.

Table 31

Annotate: compare with model group: ^*P＜0.01; ^*P＜0.05; Compare with the blank group: ^#P＜0.05.

Experimental result: the ability of learning and memory of acetyl choline content and body is proportionate, and acetylcholine esterase can reduce latter's content by the hydrolysis acetylcholine, thereby has influence on ability of learning and memory, and therefore suppressing acetylcholine esterase then can improve memory function accordingly.Activity of monoamine oxidase is closely related with the medium of regulating nerve, cardiovascular function, and the MAO-B activity increases with age growth, is an old and feeble important indicator.Behind this experimental result display model group injected in mice scopolamine, dysmnesia occur, show as that to keep away dark incubation period short.This product can obviously be improved the memory ability of mice, keeps away and obviously is longer than model group dark incubation period, and can reduce CHE content in the mouse brain, can reduce MAO content in the brain with fashion.

18, to sodium nitrite consolidate the obstacle model to mouse memory influence (water maze method):

Experimental technique:

Animal divides into groups, administration is the same.Each organizes the training of mice water maze every day once, continuously 30d.After the last administration, after each was organized mice and carries out water maze training and finish, the subcutaneous injection sodium nitrite experimentized behind the 24h immediately, and the time of mice covering the race respectively organized in record, and get brain homogenate and measure glutamic acid and MDA content.

Table 32

Annotate: compare with model group: ^*P＜0.01; ^*P＜0.05; Compare with the blank group: ^#P＜0.05.

Experimental result:

Glutamic acid is the excitatory transmitter of cone neurone, form closely related with learning and memory, but excessive glutamic acid can cause stream in sodium, calcium a large amount of, produces the neurotoxicity infringement, and participates in the pathogenesis of neural degeneration diseases such as Secondary cases neurocyte necrosis that cerebral ischemia causes, AD.MDA is the catabolite of lipid peroxide, and its content height is proportionate with cerebral tissue radical damage degree, is a kind of performance of body aging, also can cause dysmnesia simultaneously.After this experimental result shows mouse subcutaneous injection NaNO2, thereby cause mice brain anoxia to destroy the maintenance of memory, swimming time prolongs, and this product can shorten the swimming time of mice after the modeling, improving memory.Can both reduce glutamic acid content in the brain, the extract II group can also reduce MDA content in the mouse brain in addition, and the effect of antioxidant radical is arranged.

19, antifatigue effect

Get male mice and be divided into two groups at random, experimental group is irritated stomach extract 3g/kg of the present invention, and matched group is given the starch solution of equivalent, administration dropped into mice in the treadmill after 10 days, record mice entry is to the bottom, and time of unable come-up again is as swimming continuance time.

Table 33:

The result shows: this product can obviously prolong the mice swimming continuance time, has antifatigue effect.

20, mice normal pressure anoxia enduring experiment

Experimental technique:

Get the KM mice, male and female half and half are divided into 3 groups at random, i.e. blank group (giving isometric distilled water), extract I, II group.Each group is total to 30d once a day all by gastric infusion, and the administration volume is 20ml/kg.Behind the last administration 45min, mice is placed the airtight wide mouthed bottle of 125ml (built-in sodica calx 20g).Stopping with the mice mouth breathing is the dead mouse sign, the time-to-live of record mice, surpass 60min in 60min.

Table 34

Annotate: compare with the blank group: ^*P＜0.01; ^*P＜0.05.

The result shows, the time-to-live of this product energy significant prolongation mice normal pressure anoxia enduring.

21, the experimentation of small intestine movement of mice ahead running

Experimental technique:

Select the bull mice for use, body weight 18-22g is divided into blank group, model group, extract I group, extract II group at random.The same approach of blank group and model group is given distilled water, experimental group is given the various dose given the test agent, is total to 7d once a day, and water 16h is can't help in fasting after the last administration, model group and given the test agent group are irritated stomach and are given compound diphenoxylate (5mg/kg), and the blank group is given distilled water.To 30min behind the compound diphenoxylate, the given the test agent group contains the prepared Chinese ink (containing 5% activated carbon powder, 10% Radix Acaciae senegalis) of given the test agent respectively, and matched group is irritated stomach to prepared Chinese ink.Take off cervical vertebra behind the 25min immediately and put to death animal, open the abdominal cavity and separate mesentery, cut off the upper end from pylorus, lower end the intestinal tube to ileocecus, place on the pallet, gently small intestinal is pulled into straight line, measuring intestinal tube length is the small intestinal total length, is that prepared Chinese ink advances length from pylorus to prepared Chinese ink forward position.Calculate prepared Chinese ink propelling rate.

Experimental result: this product can obvious excited mice gastrointestinal peristalsis.

Table 35:

Annotate: compare with the blank group ^##P＜0.01; Compare with model control group: ^*P＜0.01, ^*P＜0.05.

Claims (10)

1. a natural plant extracts is characterized in that it being to be extracted through solvent by raw materials such as Pericarpium Vitis viniferae, haw berry, Folium Nelumbinis, Fructus Lycii, Fructus Hippophae, Herb Gynostemmae Pentaphylli, Radix Codonopsis, Radix Polygoni Multiflori Preparatas, concentrates, and merges, and the effect material that technological processes such as drying are extracted is formed.

2. natural plant extracts according to claim 1 is characterized in that the amount ranges of each component is designated as with weight:

Pericarpium Vitis viniferae 100-1000 part haw berry 10-50 part

Folium Nelumbinis 5-30 part Fructus Lycii 5-30 part

Fructus Hippophae 5-30 part Herb Gynostemmae Pentaphylli 5-20 part

Radix Codonopsis 5-20 part Radix Polygoni Multiflori Preparata 1-15 part

3. natural plant extracts according to claim 2 is characterized in that the preferred amount ranges of each component is designated as with weight:

Pericarpium Vitis viniferae 400-800 part haw berry 20-40 part

Folium Nelumbinis 10-20 part Fructus Lycii 10-20 part

Fructus Hippophae 10-30 part Herb Gynostemmae Pentaphylli 5-15 part

Radix Codonopsis 5-15 part Radix Polygoni Multiflori Preparata 1-10 part

4. natural plant extracts according to claim 3 is characterized in that the optimum amount of each component is designated as with weight:

30 parts of 600 parts of haw berries of Pericarpium Vitis viniferae

15 parts of 15 parts of Fructus Lycii of Folium Nelumbinis

10 parts of 15 portions of Herb Gynostemmae Pentaphylli of Fructus Hippophae

5 parts of 10 parts of Radix Polygoni Multiflori Preparatas of Radix Codonopsis

5. one kind contains any described natural plant extracts of claim 1-4, it is characterized in that being being prepared from according to following method:

A, independent extraction method:

A), adding 5～15 times of water gagings respectively in a certain amount of Fructus Lycii, Folium Nelumbinis, Herb Gynostemmae Pentaphylli, Radix Codonopsis decocts 2～3 times, the each decoction 1～2 hour, the water extract is concentrated, and drying obtains Fructus Lycii extract, Folium Nelumbinis extract, Herb Gynostemmae Pentaphylli extract and Radix Codonopsis extract respectively;

B), in a certain amount of Pericarpium Vitis viniferae, haw berry, Fructus Hippophae, Radix Polygoni Multiflori Preparata, add 5～10 times of amount 65～90% alcohol reflux respectively 2～3 times, the each extraction 1～2 hour, filter, discard medicinal residues, filtrate is concentrated, drying makes Pericarpium Vitis viniferae extract, Fructus Crataegi extract, Fructus Hippophae extract, Radix Polygoni Multiflori Preparata extract respectively;

C), eight kinds of extracts of step a) and step b) gained are mixed, promptly get extract provided by the present invention.

B, mixed extraction method:

A), a certain amount of Fructus Lycii, Folium Nelumbinis, Herb Gynostemmae Pentaphylli, Radix Codonopsis are mixed, adds 5～15 times of water gagings and decoct 2～3 times, decocted 1～2 hour at every turn, the water extract is concentrated, and drying obtains extract A;

B), a certain amount of Pericarpium Vitis viniferae, haw berry, Fructus Hippophae, Radix Polygoni Multiflori Preparata are mixed, adds 5～10 times of amount 65～90% alcohol reflux 2～3 times, extracted 1～2 hour at every turn, filter, discard medicinal residues, filtrate is concentrated, and drying makes extract B;

C), two kinds of extracts of A, the B of step a) and step b) gained are mixed, promptly get extract provided by the present invention.

6. the preparation method of natural plant extracts according to claim 5 is characterized in that:

A, independent extraction method:

A), adding 10 times of water gagings respectively in a certain amount of Fructus Lycii, Folium Nelumbinis, Herb Gynostemmae Pentaphylli, Radix Codonopsis decocts 2～3 times, the each decoction 1～2 hour, the water extract is concentrated, and drying obtains Fructus Lycii extract, Folium Nelumbinis extract, Herb Gynostemmae Pentaphylli extract and Radix Codonopsis extract respectively;

B), in a certain amount of Pericarpium Vitis viniferae, haw berry, Fructus Hippophae, Radix Polygoni Multiflori Preparata, add 10 times of amount 70～80% alcohol reflux respectively 2～3 times, the each extraction 1～2 hour, filter, discard medicinal residues, filtrate is concentrated, drying makes Pericarpium Vitis viniferae extract, Fructus Crataegi extract, Fructus Hippophae extract, Radix Polygoni Multiflori Preparata extract respectively;

C), eight kinds of extracts of step a) and step b) gained are mixed, promptly get extract provided by the present invention.

B, mixed extraction method:

A), a certain amount of Fructus Lycii, Folium Nelumbinis, Herb Gynostemmae Pentaphylli, Radix Codonopsis are mixed, adds 10 times of water gagings and decoct 2～3 times, decocted 1～2 hour at every turn, the water extract is concentrated, and drying obtains extract A;

B), a certain amount of Pericarpium Vitis viniferae, haw berry, Fructus Hippophae, Radix Polygoni Multiflori Preparata are mixed, adds 10 times of amount 70～80% alcohol reflux 2～3 times, extracted 1～2 hour at every turn, filter, discard medicinal residues, filtrate is concentrated, and drying makes extract B;

C), two kinds of extracts of A, the B of step a) and step b) gained are mixed, promptly get extract provided by the present invention.

7. one kind contains the application that the described natural plant extracts of claim 1 is used for preparing food, food additive, health food, medicine.

8. the application of natural plant extracts according to claim 7, it is characterized in that: be used to prepare have the control body to the absorption of taking in energy, reduce in vivo accumulation of fat, reduce the harm of superfluous nutrition body, blood fat reducing, purify the blood, blood sugar control, blocking-up and reverse sub-health state effects such as development be suitable for the food that healthy population, sub-health state crowd and ill crowd eat, the application in the food additive.

9. the application of natural plant extracts according to claim 7, it is characterized in that: be used to prepare have the control body to the absorption of taking in energy, reduce in vivo accumulation of fat, reduce the harm of superfluous nutrition body, blood fat reducing, purify the blood, blood sugar control, the nutritious and healthy food of the effects such as development of blocking-up and reverse sub-health state, the application in the health food.

10. the application of natural plant extracts according to claim 7 is characterized in that this extract can be used for preparing the application that prevents and treat the medicine of diseases such as hypertension, hyperlipemia, diabetes, obesity, fatty liver, coronary heart disease, angina pectoris, arteriosclerosis through deep processing.