KR20180037455A - Composition comprising natural herbs essential having antibiotic activity and process thereof - Google Patents

Abstract

The present invention relates to an antifungal composition comprising, as effective components, extracts of natural herbs including Angelica gigas NAKAI, Sasa borealis, green tea, Lonicera japonica, eucalyptus, and basil; and to a production method thereof. The antifungal composition is safe to the human body by containing natural herb extracts, and has a synergetic antifungal effect compared to a case of using only one extract by being produced through mixing the extracts in a predetermined ratio, and thus the composition can be used as an antifungal functional composition.

Description

TECHNICAL FIELD The present invention relates to an antifungal composition comprising a natural plant essential as an active ingredient and a method for producing the antifungal composition,

The present invention relates to an antifungal composition comprising a natural plant essential ingredient as an active ingredient and a method for preparing the antifungal composition. More particularly, the present invention relates to an antifungal composition for preventing detoxification of detoxic substances such as various kinds of heavy metals and toxic substances, ) Or an antifungal compound having excellent detoxifying effect, such as Angelica gigas Nakai, Sasagi, Green tea, Mandarin, Eucalyptus and Basil essentials, and a method for producing the same.

Fungus refers to a group of microorganisms, including fungi, yeast, and mushrooms, most of which can not synthesize their own nutrients and live on other organisms. Most people are infected with skin, such as scalp, hair, face, arms, legs, nails and claws, where cells die and peel off. Most of them are infected with gastric mucosa and vaginal mucosa. It also raises. On the other hand, infections caused by fungi are caused by immunodeficiency, old age, use of immunosuppressants or steroids, malnutrition, poor sanitary conditions, and contact with infected patients.

Angelica gigas Nakai is a perennial herb that belongs to the dragonfly family. It is composed of ingredients such as essential oil and decusin, sedative beta-sitosterol, sucrose, nicotinic acid and folic acid. It has various effects such as various female diseases, menstrual irregularities, menstrual cramps, anemia, poor uterus development, lack of postpartum blood, and menopausal disorders, and has a function to smoothly assist female hormone secretion. Recent studies have shown that Angelica gigas has an excellent effect on cellular immune responses. Oriental medicine is warm in nature, flavor is sweet and very enter the heart, liver, spleen enters. It replenishes and harmonizes the blood, regulates the physiology and makes the stools pass smoothly.

Bamboo is the smallest bamboo among bamboos, and it grows frequently in the mountains of the southern part of Korea. According to Sukyoungbogam, Boncho gangmok, Goddess of Mine and Goddess of Mine, Sasae is an amazing herb that is far superior to ginseng. It is the most powerful herb in bamboo, and its only one thing is diabetes, hypertension, gastritis, gastric ulcer, chronic hepatitis, cancer The incurable disease is often cured. In addition, it is said that the bacillus has the effect of lowering heat, releasing poison, eliminating phlegm, urinating well, treating inflammation and suppressing cancer cells.

Green tea leaves contain polyphenols called tea tannins and polyphenol oxidase, an enzyme that promotes the oxidation of these polyphenols. Tea is classified into various types such as green tea, oolong tea and black tea depending on the degree of oxidation by this enzyme. Tea has attracted much attention as a preventive medicine for cancer and cardiovascular diseases, two important goals in adult preventive medicine.

It is a perennial vine plant belonging to the Indong family and is mainly distributed in Korea and East Asia. It has been used as a treatment for infectious hepatitis, boils, etc. It has been used for treatment of fever, detoxification and perspiration.

It should be understood that the foregoing description of the background art is merely for the purpose of promoting an understanding of the background of the present invention and is not to be construed as adhering to the prior art already known to those skilled in the art.

1. Korean Patent Publication No. 10-2015-0034014 (April 02, 2015) 2. Korean Patent No. 10-0508600 (Aug. 08, 2005)

In order to solve the problems of the conventional antifungal compositions as described above, the inventor of the present invention has developed a method for producing a natural plant essential which has an excellent antifungal effect and is excellent in a detoxic effect of discharging waste materials such as various heavy metals and toxic substances in the body, On the basis of this, the present invention has been completed.

It is a main object of the present invention to provide a method for producing an antifungal composition comprising Angelica keiskei, Sasagi, green tea, Dongduche, eucalyptus and basil essentials with excellent antifungal effect.

It is still another object of the present invention to provide an antifungal composition comprising a natural plant essential substance having an excellent antifungal effect produced by the method as an active ingredient.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

In the present invention, a method for producing a natural plant essential comprises: a primary extraction of a selected natural herb or native herb plant; Extracting the primary extract in a pot; Aging only the secondary extract in the pot; And filtering the aged solution.

In the present invention, the selected natural herb or native herb plant uses Angelica gigas Nakai, Sagitta, Green tea, Dongchu, Eucalyptus and Basil.

The natural plant essentials used in the examples of the present invention are prepared and used by the following production methods.

The natural herb or native herb plant can be roughly divided into citrus fruit (fruit), flower petal, tree and root. After thoroughly washing the wood and root system, cut off at a slant so that the cross section is widely exposed so that a lot of components can be extracted. Rinse the herbs cleaned by type and rinse with spirit alcohol, then mix the natural plant oil with the same type of herbal ingredients: alcohol alcohol in volume ratio of 1: 1. In particular, the petal system can be mixed with natural vegetable oil: alcohol in a volume ratio of 2: 1 in view of the water content and the easily scratched nature.

In the present invention, eucalyptus oil or basil oil is used as a natural plant oil used as a solvent in the first extraction step.

At this time, eucalyptus oil or basil oil and alcohol alcohol can be mixed in a volume ratio of 1: 1.

The eucalyptus oil and the basil oil used in the present invention are extracted from plants and can be produced by a known extraction method as an essential oil used for perfume or aroma therapy and are not limited to a specific production method.

Eucalyptus oil (Eucalyptus globulus Oil) is sharp and refreshing, cleanses the head, enhances respiratory function. It has antiviral action and is effective for influenza, throat infection, cough, Qatar symptoms, sinusitis, asthma, pulmonary tuberculosis.

Ocimum Basilicum Oil has a fresh, clean scent, and is effective in improving mood, concentration and depression. Tenderness, germane, dryness, aphrodisiac, and antidepressant.

In the primary extraction step, the alcohol extraction method, cold compression method, and high concentration natural plant oil aging method are used because not only water-soluble but also fat-soluble components are extracted. Preferably, the alcohol extraction method is used by mixing the natural vegetable oil and the alcohol in a volumetric ratio of 1: 1 or 2: 1. It is used as a safe container that does not release environment hormones. It is sealed with wax so that the natural plant fragrance does not volatilize after extraction, and then stored in a cool, shady place. At this time, the temperature is maintained in the range of 15 to 20 ° C, but it is stored at not more than 20 ° C, and the first extraction step can be carried out for 50 to 55 days.

The second extraction step can be carried out for 24 months. The first extract is crushed and transferred to Onggi (soil earthenware), sealed with wax as in the first extraction step, and then 2/3 or more Bury and store in a cool, cool place at 15-20 ° C. It is good to adjust the temperature. If the Onggi is not tightly sealed with wax, the fragrance may be volatilized or the outside air may enter and deteriorate.

In the aging step, the herb or herb and the natural plant essential are separated from the used extract to the second extraction, and the extract (solution) is aged by putting it in a pot (soil earthenware). This step should be kept in a cool place where the temperature does not rise above 20 ℃ because the proper temperature is important, and keep it sealed for at least 24 months. The longer the ripening period, the higher the natural plant essentials of high concentration. Since the algae contained in the extract are mostly volatilized as the aging period passes, the content of alcohol is low in pure natural plant essentials because it is aged in Onggi (soil earthenware).

In the filtration step, the solution (natural plant essential) obtained through the aging step was filtered using a 5 μm filter paper Use a vacuum filter to filter out fine impurities.

In the present invention, natural plant essentials can use Angelica giganteus essential, Sasa essential, Green tea essential, and Indochina essential.

The antifungal composition containing the natural plant essential substance of the present invention as an active ingredient is a natural plant essential substance prepared by the natural plant essential production method, and it is used as Angelica essential oil, Sage essential oil, Green tea essential oil and Indochina essential oil, May be prepared by blending or aging a mixture of basil oil.

In one embodiment of the present invention, 15% by weight of Angelicae essential, 15% by weight of Sasa essential, 15% by weight of green tea essential, 15% by weight of Essential essential oil, 20% by weight of eucalyptus oil and 20% .

The antifungal composition of the present invention can be manufactured in the form of general functional cosmetics, external preparation, food preservative, spray.

In one embodiment, when the antifungal composition of the present invention is prepared into a spray form and sprayed on futon, it may exhibit an antifungal effect as well as a sterilizing effect and a house dust mite eradication effect.

Natural plant therapy is also called fragrance therapy, and fragrance enters the limbic system of the brain within 3 seconds through the olfactory nerve. Actual usage may be inhalation, massage or eating.

The natural plant essential of the present invention has an excellent antifungal effect because of its excellent antimicrobial (antibacterial, antifungal, antiviral) function and can exhibit a relaxing effect which alleviates mental and physical tension.

The natural plant essentials according to the present invention can be divided into seven functional groups such as phenol series, oxide series, terpene series, ester series, aldehyde series, ketone series and alcohol series. Due to the difference in contents contained in each natural plant selected , Physiological activity, sterilization or antifungal, and detoxifying effect.

As described above, the present invention can be used as an antifungal functional composition because it contains a natural plant essential substance and is safe to human body and can be produced by mixing at a certain ratio, thereby exhibiting a synergistic antifungal effect rather than a single essential substance.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a photograph showing the results of an antifungal effect test of Experimental Example 2 of an antifungal composition containing a natural plant essential of the present invention. FIG. (a) Halo test: P. acnes , 72 hours, (b) Halo test: M. furfur , after 48 hours.

Hereinafter, the present invention will be described in more detail with reference to Examples.

It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

<Angelica Essential  Manufacturing>

Angelica gigas were grown for more than 5 years and roots were picked, washed, and dried to remove impurities, dried naturally or dried and then immersed in a fermented juice. A solvent for primary extraction was prepared by mixing eucalyptus oil and alcohol alcohol in a 1: 1 volume ratio. The Angelica japonica is placed in a pot, and the extracting solvent is poured to the extent that the Angelica gigas is submerged. After extraction, the gelatin is sealed with wax to prevent volatilization of the Angelica japonica, followed by incubation in a cool shady place for 50 to 55 days Respectively.

The above-mentioned first-extracted Angelicae Radix extract was crushed and transferred to Onggi, sealed with beeswax in the same manner as in the first extraction step, and then buried in the ground at least 2/3 of its length, and placed in a shady, cool place at a temperature of 15 to 20 ° C for 24 months Respectively.

The extracted Angelica japonica extract was used to separate the extracted extract. The extract was kept in a sealed place for more than 24 months in a cool place where the extract was not raised above 20 ° C. The aged Angelica japonica essential solution was filtered using a 5 μm filter paper The filtrate was filtered using a vacuum filter to filter out fine impurities.

<Sasae Essential  Manufacturing>

Sasa was washed and dried to remove impurities and dried to dryness, or dried and then immersed in a fermented alcoholic beverage. A solvent for primary extraction was prepared by mixing eucalyptus oil and alcohol alcohol in a 1: 1 volume ratio. The sage was placed in a pot, the extraction solvent was poured to such an extent that the sage was submerged, and then the sage essential was prepared in the same manner as in the preparation of the Angelica japonica essential.

<Green tea Essential  Manufacturing>

Green tea was prepared by taking tea leaves, washing them to remove impurities, drying them naturally to dry them, drying them, and soaking them in a fermented alcoholic beverage. A solvent for primary extraction was prepared by mixing eucalyptus oil and alcohol alcohol in a 1: 1 volume ratio. The rusty tea leaves were placed in a pot, and the extraction solvent was poured to such an extent that the rusty tea leaves were submerged. Then, green tea essentials were prepared in the same manner as in the preparation of the Angelica japonica essential.

<Dong Dong Essential  Manufacturing>

The leaves were picked and then washed to remove impurities, dried naturally to dry the water, or dried and then immersed in a fermented juice. A solvent for primary extraction was prepared by mixing eucalyptus oil and alcohol alcohol in a 1: 1 volume ratio. The seeds of the seedlings were placed in pots, the extracting solvent was poured to such an extent that the seeds of the seedlings were locked, and then the seedlings were prepared in the same manner as in the preparation of the seedlings.

&Lt; Example 1 >

15% by weight of Angelica essential oil, 15% by weight of balsam essential oil, 15% by weight of green tea essential oil and 15% by weight of indochine essential oil, 20% by weight of eucalyptus oil and 20% by weight of basil oil were matured for 1 day, An antifungal composition was prepared.

< Experimental Example  1 > Antifungal effect test of the antifungal composition of the present invention

Test strain Escherichia Coli ATCC 25922 and Staphylococcus aureus ATCC 6538 were subjected to an antifungal activity test of the antifungal composition of the present invention prepared in Example 1, and the test results are shown in Table 1 below. The number of the initial bacteria was measured, and the number of bacteria after the lapse of 10 minutes was measured after the antifungal composition of the present invention prepared in Example 1 was treated.

Reduction rate (%) = {(A-B) / A} x 100

(A: initial number of bacteria, B: number of bacteria after a certain time)

Test strain
(CFU / ml) Sample classification result E. coli Early 5.2 x 10 ⁵ After 10 minutes &Lt; 10 (99.9% or more) S. aureus Early 3.8 x 10 ⁵ After 10 minutes &Lt; 10 (99.9% or more)

As shown in Table 1, the test strain Escherichia Coli ATCC 25922 and Staphylococcus aureus ATCC 6538 were measured to be 10 CFU / ml or less after 10 minutes. Therefore, the antifungal composition of the present invention showed an antifungal effect of 99.9%.

< Experimental Example  2 > Antifungal effect test of the antifungal composition of the present invention

In order to examine the antibacterial and antifungal effect of the antifungal composition of the present invention, antifungal activity tests of the antifungal composition of the present invention prepared in Example 1 were conducted using P. acnes and M. furfur . This test was conducted according to the Halo test method.

(1) Propionibacterium acnes test method

P. acnes was inoculated into Fluid thioglycollate mediu medium and cultured at 35 ± 1 ℃ for 7 days.

The P. acnes culture was inoculated on a pre-prepared Brewer's anaerobic agar plate with a sterile cotton swab, treated with 25 μl of the stock solution in a sterilized paper disc (8 mm), placed on the center of the medium The zone of inhibition produced by anaerobic jar incubation at (72 ± 2) hours in anaerobic condition at 35 ± 1 ℃ was measured.

(2) Malassezia furfur Test Methods

Malassezia Furfur was inoculated into brain heart infusion (BHI) and cultured at (30 ± 1) ° C for 48 ± 2 hours. The medium was centrifuged at 3,000 rpm for 10 minutes, and the medium was removed. The medium was washed once with sterile physiological saline, centrifuged again, and the supernatant was removed and suspended in sterile physiological saline.

The M. furfur spore suspension was inoculated uniformly onto the prepared LNA plate medium with sterilized cotton swabs, treated with 25 μl of the stock solution in a sterilized paper disc (8 mm), placed on the center of the medium 30 ± 1) ℃ for 48 ± 2 hours to measure the diameter of the zone of inhibition.

(3) Results

Test strain T D W P. acnes 12.0 8.0 2.0 M. furfur 10.0 8.0 1.0

Growth Low Zone: W = (T-D) / 2

Where W is the width of the low zone (mm), T is the total diameter of the sample and the low zone (mm), D is the diameter of the sample (mm)

(4) Results

As shown in Table 2 above, a low zone of 2.0 mm for P. acnes and 1.0 mm for M. furfur was formed under the presented conditions. That is, the antifungal composition of the present invention showed antibacterial effect against P. acnes and antifungal effect against M. furfur .

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (2)

15% by weight of Angelica essential oil, 15% by weight of Sasa essential, 15% by weight of green tea essential and 15% by weight of Echinacea essential oil, 20% by weight of eucalyptus oil and 20% by weight of basil oil.
The method according to claim 1,
Wherein said Angelica essential, berry essential, green tea essential and mandarin essential are produced by the following steps:
(a) first extracting the selected natural herb or native herb;
(b) extracting the first extract of step (a) in a pot by secondary extraction;
(c) aging only the secondary extract of step (b) in Onggi; And
(d) filtering the aged solution of step (c).

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