KR20180031309A - Composition for moisturizing skin and restoring damaged skin comprising panthenol and Pinus koraiensis - Google Patents

Abstract

The present invention relates to a composition containing panthenol and Pini Koraiensis Semen oil, and more particularly, to a composition containing panthenol and Pini Koraiensis Semen oil together, which simultaneously provides a synergistic effect between the panthenol and Pini Koraiensis Semen oil, thereby exhibiting effects of skin moisturizing, restoring of damaged skin due to the lowered skin barrier function. Therefore, the composition is preferably applicable as an external preparation and various cosmetic products.

Description

TECHNICAL FIELD The present invention relates to a composition for restoring skin moisturization and skin damage including panthenol and salvia oil,

The present invention relates to a cosmetic composition and an external preparation for skin to improve skin moisturizing ability by synergy effect of panthenol and saliva oil and to restore skin damage due to skin barrier function deterioration.

The skin is histologically composed of three layers of epidermis, dermis, and subcutis. The outermost part of the epidermis is located at the stratum corneum, and its constituent is keratinocytes and skin lipids, and functions as a skin barrier function.

Skin barrier function is an important function to protect skin from external harmful substances by controlling cell division and differentiation of keratin, prevent leakage of substances in body, and prevent evaporation of skin moisture.

In the case of good skin, the water content in the stratum corneum starts to increase from 15%, and gradually increases toward the granular layer. However, in the case of dry skin, the moisture content decreases drastically as the skin gets closer to the epidermis, .

Due to lack of water in the stratum corneum, the skin is excessively proliferated in order to maintain homeostatic properties. As a result, the skin becomes dry and the skin barrier becomes damaged and the skin becomes rough and loses its transparency. Dry skin appears and causes chronic skin diseases such as atopy do.

Moisturizers such as hand creams and body lotions are used to increase the moisture in the stratum corneum.

Moisturizers have the function of occlusives, humectants and emollients, which reduce water replenishment and transepidermal water loss (TEWL) when applied to dry skin. These moisturizers simply cover the skin and keep moisture in it. Recently, however, attention has been focused on restoring and / or strengthening the barrier function, which is the most important function of the skin, rather than merely replenishing moisture.

Various combinations of compositions have been proposed with respect to moisturizing and enhancing skin barrier function. For example, Korean Patent Publication No. 2005-0097578 discloses a cosmetic composition comprising a jujube extract and a walnut extract, which accelerates the differentiation of the stratum corneum by keratinocytes, restores the barrier function of damaged skin, And that it has a better effect on maintenance.

Korean Patent Laid-Open Publication No. 2015-0093300 discloses a cosmetic composition containing scopoletin as an active ingredient and having excellent skin barrier function and moisturizing power.

The skin barrier function and moisturizing power can not sufficiently be secured even in the presentation of various compositions, and development of a composition having such an effect is urgent.

Korean Patent Publication No. 2005-0097578 (Oct. 10, 2005), cosmetic composition for moisturizing skin containing jujube extract and walnut extract Korean Patent Laid-Open Publication No. 2015-0093300 (Aug. 2015), cosmetic composition for enhancing skin barrier function containing scopollen as an active ingredient

In the present invention, various candidate substances were selected and screened to simultaneously provide a moisturizing function and a skin barrier function-enhancing effect, and as a result, panthenol and sea grass oil were selected, and when they were mixed in a specific range, The present invention has been accomplished on the basis of confirming that it is possible to dramatically improve the stability and formulate the formulation at the same time.

Accordingly, an object of the present invention is to provide a cosmetic composition which can simultaneously provide a moisturizing function and a skin barrier function enhancing effect.

In order to achieve the above object, the present invention provides a composition for restoring skin moisturization and skin damage, which comprises panthenol and a saliva oil as an active ingredient.

In this case, the panthenol and the sea oil are mixed at a weight ratio of 0.1 to 10: 0.1 to 20.

Wherein the composition is a cosmetic composition or a composition for external application for skin.

The cosmetic composition according to the present invention includes panthenol and saliva oil at the same time, and can be suitably applied to various cosmetics and external skin preparations with skin moisturizing and strengthening effect as well as skin damaging function due to skin barrier function deterioration due to the effect of the liver barrier .

(A) is Involucrin, (b) is Loricrin, (c) ABCA12, and (d) is a graph showing the skin moisturizing and skin barrier function enhancing ability of dexpen- ) Is a graph showing the degree of mRNA expression for CERS3.
FIG. 2 is a graph for confirming skin moisturizing and skin barrier function enhancement ability of salvia oil. FIG. 2 (a) is a graph showing the degree of PPAR-.gamma.-activation of PPAR-.alpha.-activated (b).

In the present invention, a composition having enhanced skin moisturizing and skin barrier functions through synergy between panthenol and sea grass oil is proposed.

Panthenol is a derivative of vitamin B (pantothenic acid) and has a liquid form with a colorless odorless transparent high viscosity at room temperature. The panthenol has two isomers of D-Panthenol and L-Panthenol, and D-Panthenol (or Dext Panthenol) of Formula 1 and D, L-Panthenol of Formula 2 have biological activity.

Panthenol referred to herein includes both D-Panthenol and D, L-Panthenol, unless otherwise noted.

Panthenol is a penetrating, non-irritating moisturizer that improves the moisturizing and maintaining function of the skin and stimulates skin regeneration. It has the effect of making the dry skin soft and elastic, improving the skin moisturizing function, and preventing inflammation and itching.

1 is a graph for confirming the effect of panthenol on moisturizing / skin barrier strengthening, wherein (a) is Involucrin, (b) is Loricrin, (c) ABCA12, mRNA expression level. 1, panthenol alone has a certain degree of skin moisturizing and skin barrier function improving effect.

Moisturizing moisturizes the skin through the supply of water. Even if the moisture is continuously supplied, it is difficult for moisture to remain on the skin for a long time. If possible, moisturizing care to balance the moisture in the skin should be performed together with skin barrier function It is advantageous.

Therefore, various oils were selected as a substance to regulate the water balance in the skin, and a substance which obtained a synergistic effect on skin moisturizing and skin barrier function enhancement when mixed with panthenol was selected.

Panthenol has a moisturizing effect by skin moisturizing and skin barrier function. Usually, oil has a moisturizing effect by oil film formation. However, they exhibit hydrophilic and lipophilic characteristics, respectively. When these two are mixed, There is a possibility that the stability of the liquid crystal display device is greatly reduced.

However, according to the present invention, salted fish oil was selected as an oil component and, as a result of mixing with panthenol, superior skin moisturizing and skin barrier function could be secured compared with the case where each of panthenol and salted fish oil was used alone.

Pinus koraiensis seed oil is a nonvolatile oil extracted from the seeds of Pinus koraiensis and is known to have moisturizing, anti-aging and sedative effects. The above-mentioned sea grass oil forms a moisturizing oil film on skin without skin glare, and regulates the water balance in the skin by capturing moisture flowing from the skin, thereby further enhancing the skin moisturizing and skin barrier function of panthenol.

The sea grass oil used in the present invention can be purchased commercially or directly extracted.

Upon direct extraction, the saline oil can be extracted by conventionally known plant oil extraction methods such as high temperature compression, supercritical extraction or organic solvent extraction using an organic solvent.

In the examples of the present invention, salted fish oil obtained through an organic solvent extraction method is used, and an excessive amount of salted fish oil can be obtained by such an extraction method. In this case, various organic solvents such as hexane, methanol, ethanol, butanol, isopropanol, chloroform, acetone, and acetonitrile may be used as the organic solvent, but hexane is most preferably used in consideration of the extraction efficiency and the content of the active ingredient.

Specifically, the sea grass oil is prepared by washing the pine nut, drying it, pulverizing it into a powder or a granular state, adding hexane to the sea grass powder at 1 to 50 times (weight ratio) Stir for 72 hours. After stirring, the extraction solution is filtered to recover the salvage oil, followed by concentration, followed by one or more purification steps to obtain clear and transparent sea oil. At this time, a well-known process such as column chromatography can be used for purification.

Specifically, the composition according to the present invention can dramatically improve skin moisturizing and skin barrier function when panthenol and saliva oil are mixed at a weight ratio of 0.1 to 10: 0.1 to 20.

According to the preferred examples and comparative examples of the present invention, when the panthenol and the sea grass oil are used respectively, the water content of the panthenol increases, and the sea grass oil has the higher oil content. It was confirmed that a high moisturizing effect can be secured.

Even if these two are mixed, more stable formulation can be achieved when the content ratio is satisfied. That is, when the content of panthenol is less than the above-mentioned content, sufficient moisturizing and skin barrier improvement effects can not be secured. On the contrary, when the content is higher than the above-mentioned content ratio, formulation stability is lowered.

At this time, each of the panthenol and the saliva oil is contained in an amount of 1 to 10% by weight, and 0.001 to 5% by weight within 100% by weight of the total composition, which is advantageous in securing the above-mentioned effects and in the formulation stability.

The composition comprising the panthenol and saliva oil according to the present invention can be used for moisturizing the skin and / or enhancing the skin barrier function. In particular, it can be used as a skin dryness, atopic dermatitis, contact dermatitis or psoriasis Can be effectively used as a cosmetic composition and a composition for external application for skin.

More specifically, the cosmetic composition according to the present invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. The ingredients contained in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the above-mentioned composition as an active ingredient. Examples of the ingredients include moisturizers, softeners, surfactants, organic and inorganic pigments, organic powders, Antiseptics, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, blood circulation accelerators, coolants, antiperspirants and the like.

Examples of the moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = Polyglycerin B, polylactic acid, lactic acid, cholesterol, cholesterol ester, carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, Examples of the hydrophilic polymer include hydroxypropyl cellulose, hydroxypropyl cellulose, hydroxypropyl cellulose, hydroxypropyl cellulose, hydroxypropyl cellulose, hydroxypropyl cellulose, hydroxypropyl cellulose, hydroxypropyl cellulose, hydroxypropyl cellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, hydroxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, dextrin, polyvinylpyrrolidone / , Dextrin fatty acid esters, polymer silicon, and the like.

In the present invention, cosmetics having various emulsions of W / O, O / W, W / O / W and O / W / O types including panthenol and salvia oil can be manufactured.

Here, examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self emulsifying monostearic acid glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, polyoxyethylene (POE) sorbitan fatty acid ester, polyoxyethylene (POE) Sorbitol fatty acid esters, polyoxyethylene (POE) glycerin fatty acid esters, polyoxyethylene (POE) alkyl ethers, polyoxyethylene (POE) fatty acid esters, polyoxyethylene (POE) hardened castor oil, polyoxyethylene (Polyoxyethylene / polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, lauric acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid But is not limited to.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, alkyl Amide phosphate, alkyloyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkylsulfosuccinate, sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester But is not limited thereto.

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium bromide, But are not limited to, benzalkonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, and quaternary ammonium salts of lanolin derivatives.

Examples of the amphoteric surfactant include carboxybetaine, amidebetaine, sulfobetaine, hydroxysulfobetaine, amidosulfobetaine, phosphobetaine, aminocarboxylate, imidazoline derivative, amideamine, etc. , And the like.

Also, the thickening agent is used to impart a proper viscosity to the product and stabilize and strengthen the final formed W / O / G network. In the present invention, cellulose gum, xanthan gum, gellan gum, agar, carbomer, Hydroxypropylcellulose, hydroxyethylcellulose, and hydroxyethylcellulose.

Most preferably, it is most advantageous in terms of formulation stability to use a non-ionic emulsifier as a surfactant and a high-alcohol having an amphipathic acid as an auxiliary emulsifier in appropriate ratios so as to prescribe and formulate an increasing agent in a minimized form.

The cosmetic composition of the present invention is not limited to the formulations and may be in the form of a formulation known in the art.

For example, the formulations of the cosmetic composition according to the present invention may be formulated as skin lotions, skin softeners, skin toners, astringents, lotions, milk lotions, water lotions, nutrition lotions, creams, massage creams, , Nourishing essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body cream, body lotion, body oil, body essence or body cleanser.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

In addition, the panthenol and saliva oil proposed in the present invention can be applied as a composition for external application for skin, and has an effect of preventing or improving dry skin, atopic dermatitis, contact dermatitis or psoriasis.

When used as a composition for external application for skin, it may be formulated containing an acceptable carrier, medium or base in the field of dermatology. At this time, the composition may contain a skin absorption promoting substance to increase skin moisturizing effect, skin barrier function enhancing effect, and skin keratinocyte differentiation inducing effect.

In addition, the composition for external application for skin may be in the form of a lipid, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, Any other ingredients commonly used in ionic or nonionic emulsifiers, fillers, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic active agents, lipophilic active agents, lipid vesicles or external preparations for the skin , ≪ / RTI > and the like. Wherein the ingredients can be introduced in amounts commonly used in the field of dermatology.

The composition for external application for skin includes, but is not limited to, ointments, plasters, lotions, liniments, emulsions, aerosols, extracts, fludextracts, gels, A tablet, a wetting gel, a suspension, an emulsion, a paste, a spray, a patch, a patch, and the like.

In the composition for external application for skin, the effective amount of the skin antioxidant composition according to the present invention varies depending on the composition of the external preparation for skin, the kind of the formulation, the age, body weight, health condition, sex, administration time, .

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

EXPERIMENTAL EXAMPLE 1 Analysis of Protein and Enzyme Related Gene Expression of Panthenol

To confirm the effect of panthenol on skin barrier function, human keratinocyte line HaCaT cells were used to confirm gene expression level involved in skin barrier function.

Involucrin, Loricrin, ABCA12 and CERS3 gene expression were confirmed. Involucrin is a protein that forms a membrane that protects keratinocytes in association with loricrin. Rorikreen is a protein that plays an important role in Epidermis differentiation. ABCA12 is important for the normal development of skin cells. Cells such as ceramides , And CERS3 is an enzyme that synthesizes ceramides that are important for skin barrier composition.

HaCaT was cultured in DMEM medium (Dulbecco's modified Eagle's Medium, Gibco 1210-0038) containing 10% fetal bovine serum. 1x10 < ⁶ > cells were dispensed into T-75 flasks and cultured for 24 hours. Then, DMEM medium without fetal bovine serum was treated, cultured for 24 hours, and then treated with panthenol for 24 hours in a medium containing no fetal bovine serum. The cells were then harvested, washed with cold phosphate buffered saline (PBS), and 1 mL of the TRIZOL reagent (Life Technologies, Inc.) was added to extract the total RNA and quantitated Reverse transcription PCR was performed. PCR experiments confirmed the expression of the four target genes.

4 쨉 g of total RNA was added to 25 쨉 l of a reverse transcription reaction buffer containing 50 mM Tris-HCl (Tris-HCl, pH 8.3), 75 mM KCl, 3 mM MgCl ₂ , 0.1 M DTT, 10 mM dNTP, 40 units / R RNase inhibitor, (DT) 16 primer and a 200 unit SuperScript II (SuperScript II, GiboBRL) reverse transcription polymerase were added and reacted at 42 ° C for 1 hour. 2.5 μl of the reverse transcription reaction solution was then diluted with AmpliTaq DNA polymerase (0.04 U, Perkin Elmer, Shelton, CT), 50 mM Tris (pH 8.3), 0.25 mg / Was added to 50 L of a PCR reaction buffer containing 3 mM MgCl ₂ , 0.25 mM dNTPs and 1 / 50,000 dilution of SYBR Green I [Molecular Probes, Eugene, Ole (Molecular Probes, Eugene, OR)] and 10 μM of primer 30 C for 30 seconds, 53 C for 30 seconds, and 72 C for 1 minute.

Relative mRNA levels were analyzed by measuring changes in SYBR Green I fluorescence using ICycler software and are shown in FIG. As a negative control group, relative mRNA levels were shown based on the value of 1 in the ethanol treatment group.

1 is a graph for confirming the effect of enhancing skin barrier function of dexpentanol, wherein (a) shows the degree of mRNA expression of Involucrin, (b) roricrin, (c) ABCA12, and (d) CERS3 Graph. At this time, CTL is a control group and means a group not treated with dexpanthenol.

Referring to FIG. 1, it can be seen that panthenol has a significant gene expression-enhancing effect on each protein and enzyme, and thus has a skin barrier function.

Experimental Example  2: Sea lion  Oil PPAR -α, PPAR -γ activation effect analysis

Activation of PPAR-α and PPAR-γ, which are involved in the skin barrier function, was examined using human keratinocyte line HaCaT cells to confirm skin moisturizing and skin barrier function of sea tangle oil.

PPAR-a and PPAR-y are involved in maintaining epidermal homeostasis by inducing normal skin layer formation by controlling epidermal keratinocyte differentiation and lipid metabolism.

HaCaT, a human keratinocyte, was subcultured in a DMEM medium containing 10% fetal bovine serum and phenol red-free medium was used to remove the effect of estrogen on phenol red Respectively. Plasmids are PPARs response elements (PPARs) that are activated by binding of PPAR-α, PPAR-β / δ, and PPAR-γ plasmid with ligand-binding PPARs after a universal promoter, A firefly luciferase gene which acts as a reporter with a promoter as a reporter and a universal promoter to be used as a reference to a β-galactosidase gene Lt; / RTI > plasmid was used [Clew, S. Kliewer, S. A.), Saturn, Rain. Forman, B.M., Blumberg, B., Ong, Lee. Ong, E. S., Borgmeyer, U., Mannelsdorf, D .; Mangelsdorf, D. J., Umesono, K., Evans, Al. Evans, R. M., Differential expression and activation of a family of murine peroxisome proliferator-activated receptors, Proc. Natl. Acad. Sci. USA, 91 (1994) 7355-7359.].

HaCaT cells were plated in a 24 well plate at a concentration of 5x10 < ⁴ > and cultured for 24 hours. Then, the plasmid gene was transiently transfected. After incubation for 24 hours, the cells were washed with phosphate buffered saline (PBS), treated with 10 μM of salicylic acid and Wy-14,643 and PPAR-γ ligand troglitazone ("TGZ") as positive controls . The negative control group was a group treated with ethanol used for dissolving the sample. After culturing for 24 hours, the cells were washed with PBS and the luciferase activity was measured by harvesting the cells.

FIG. 2 is a graph for confirming the skin barrier function enhancing effect of salvia oil. FIG. 2 (a) is a graph showing PPAR-.alpha. Activation (b) showing PPAR-gamma activation level. In this case, CTL means control group, and 10 micromolar WY (positive control substance of WY14643-PPAR-α) / TGZ (positive control substance of troglitazone-PPAR-γ) positive control.

Referring to FIG. 2, it was confirmed that saliva oil effectively induced significant levels of PPAR-a and PPAR-y activity.

Example  1 to 3, Comparative Example  1 to 4

In order to confirm the synergy effect related to skin moisturization and skin barrier function enhancement by combination of panthenol and saliva oil, it was formulated into a nutritional cream as shown in Table 1 below.

The raw materials 2, 3, 4, 5, 6 and 7 shown in the following Table 1 were added to a mixer and stirred at 70 캜 to prepare an oily portion. The raw materials 1, 8, 9, 10 and 11 shown in the following Table 1 were added to a separate mixer, and the mixture was stirred at 70 ° C to prepare an aqueous phase.

Next, the prepared oily phase was gradually added dropwise to the top of the water and stirred at 8000 rpm for 3 minutes to prepare an emulsion. Here, raw materials 12 and 13 were added, and homomixing was performed again at 7000 rpm for 2 minutes to induce the increase and dispersion. The obtained emulsion was cooled to 30 DEG C, and then a nutritive cream was produced through degassing and discharging.

Composition (% by weight) Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 One Dexpanthenol 5 10.02 5.01 0 5 5 2 Seaweed oil 0.01 0 0 5.01 0 0 3 Jojoba oil 0 0 0 0 0.01 0.02 4 Polyglyceryl-3 methyl glucoside distearate 2 2 2 2 2 2 5 Cetyl alcohol 3 3 3 3 3 3 6 PEG-100 stearate 0.8 0.8 0.8 0.8 0.8 0.8 7 Dimethicone 5 5 5 5 5 5 8 water to 100 to 100 to 100 to 100 to 100 to 100 9 Gt; EDTA < / RTI & 0.05 0.05 0.05 0.05 0.05 0.05 10 glycerin 12 12 12 12 12 12 11 Ethylhexyl glycerin 0.05 0.05 0.05 0.05 0.05 0.05 12 Polyacrylate-13 * polyisobutene * polysorbate 20 0.6 0.6 0.6 0.6 0.6 0.6 13 Spices 0.4 0.4 0.4 0.4 0.4 0.4

Experimental Example  3: Skin Moisture power  Synergy measurement

Skin pulling and skin moisture contents were measured to confirm skin moisturizing effect of the cream formulations prepared in the above Examples and Comparative Examples.

(1) Skin pull measurement

The skin pull index was analyzed using a cutometer. The measurement value of the cutometer is expressed by a value (mm, ratio) between 0 and 1, and the higher the value, the better the skin pulling. The growth rate was calculated as (immediately after application - prior to application) / before application * 100.

Before application Immediately after application Growth rate (%) Example 1 0.173 0.194 12% Comparative Example 1 0.173 0.180 4% Comparative Example 2 0.176 0.183 3.9%

Referring to Table 2, it can be seen that the cream according to the present invention can improve skin pulling after application of the skin.

(2) Measurement of skin moisture

The face of 60 males and females in the 40s and 50s classified as dry skin was washed and then subjected to hot air drying to apply the cream formulations of the above Examples and Comparative Examples. The skin moisture content was measured by a skin moisture meter (Corneometer CM825, C + K Electronic Co., Germany) immediately after cleaning, after hot air drying, and immediately after application under constant temperature and humidity conditions (24 ° C., relative humidity 40%).

The results in Table 3 are based on the skin moisture meter measured immediately before the start of the test, and the percent increase in the measured value after treatment for a certain period of time. At this time, the control group means that the cream formulation is not applied.

Skin moisture content Immediately after cleaning After hot air drying Immediately after application Decrease rate immediately after washing vs hot air drying (%) After hot air drying vs Decrease immediately after application (%) Example 1 45.718 32.348 67.505 28.734 110.375 Comparative Example 1 45.730 32.008 58.680 29.017 90.471 Comparative Example 2 45.722 31.980 57.912 29.145 91.590 Control group 45.721 31.870 32.220 29.808 1.110

Referring to Table 3, it can be seen that the composition of the cream formulation according to the present invention maintains a high level of moisture content even after drying.

Experimental Example 4: Enhancing effect of skin barrier function

The skin barrier function enhancing effect of the cream formulations prepared in the above Examples and Comparative Examples was measured as follows.

In order to measure the effect of the composition according to the present invention on the recovery of damaged skin barrier function due to skin damage, the following experiment was conducted. The recovery of transdermal water loss (TWEL) by applying the cream of Examples and Comparative Examples for 6 weeks while applying the cream of Examples and Comparative Examples to the skin barrier by damaging the skin barrier using the tape stripping method for 10 adults, Were measured and compared with a Vapometer (Delfin, Finland). At this time, a commercially available moisture cream (Fiji gel) was purchased and used as a control group.

Percutaneous water loss Before application 2 weeks after application 4 weeks after application Reduction rate after 2 weeks Decrease rate after 4 weeks Example 1 30.191 23.286 21.941 20.033 24.273 Comparative Example 1 29.557 24.024 23.470 - - Control group 27.536 24.623 23.418 9.571 13.466

Referring to Table 4, it can be seen that the cream according to the present invention has a small amount of transdermal water loss and can effectively improve the skin barrier function.

Hereinafter, another formulation example of the present invention will be described, but the formulation of the composition comprising dexpanthenol and saliva oil according to the present invention is not limited to these examples.

Formulation Example  1: Massage cream

Massage creams comprising the panthenol and saliva oil of the present invention were prepared in a conventional manner according to the composition of Table 8 below.

ingredient weight% Panthenol 5.0 Seaweed oil 0.01 Icari seed B2 0.5 Wax 10.0 Polysorbate 60 1.5 PEG 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Remainder

Formulation Example 2: Nourishing lotion

Nutrition lotions containing the panthenol and saliva oil of the present invention were prepared in a conventional manner according to the composition of Table 9 below.

ingredient weight% Panthenol 5.0 Seaweed oil 0.01 Alpha-keto glutaric acid 1.0 Niacinamide 1.0 Beta-1,3-glucan 1.0 Wax 4.0 Polysorbate 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservative, fragrance 0.1 Purified water Remainder

Formulation Example 3: Pack

A pack containing the skin antioxidant composition of the present invention was prepared in a conventional manner according to the composition of Table 10 below.

ingredient weight% Panthenol 5.0 Seaweed oil 0.01 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 ethanol 6.0 Purified water Remainder

Formulation Example 4: ointment

Ointments containing the panthenol and saliva oil of the present invention were prepared in a conventional manner according to the composition of Table 6 below.

ingredient weight% Panthenol 5.0 Seaweed oil 0.01 Alpha-keto glutaric acid 1.0 Niacinamide 1.0 Beta-1,3-glucan 10.0 Wax 10.0 Polysorbate 5.0 Piggy 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.5 Vaseline 5.0 Liquid paraffin 10.0 Squalane 5.0 Sheer butter 3.0 Caprylic / capric triglyceride 5.0 glycerin 10.0 Propylene glycol 10.2 Triethanolamine 0.2 Preservative, fragrance 0.1 Purified water Remainder

Formulation Example 5: Gel

A gel containing the panthenol and saliva oil of the present invention was prepared in a conventional manner according to the composition of Table 7 below.

ingredient weight% Panthenol 5.0 Seaweed oil 0.01 Alpha-keto glutaric acid 1.0 Niacinamide 1.0 Beta-1,3-glucan 0.1 Ethylenediamine sodium acetate 0.05 glycerin 5.0 Carboxyvinyl polymer 0.3 ethanol 5.0 Piggy 60 hardened castor oil 0.5 Triethanolamine 0.3 Preservative, fragrance 0.1 Purified water Remainder

The composition containing panthenol and saliva oil according to the present invention has a skin damaging function and a skin damaging recovery effect due to deterioration of skin barrier function, so that it can be suitably applied to various cosmetic compositions and compositions for external application.

Claims (13)

Wherein the cosmetic composition comprises a panthenol and a saline oil. The method according to claim 1,
Wherein the panthenol is D-Panthenol, D, L-Panthenol or a mixture thereof. The method according to claim 1,
Wherein the panthenol is contained in an amount of 1 to 10% by weight in 100% by weight of the total composition. The method according to claim 1,
Wherein the saliva oil is contained in an amount of 0.005 to 1 wt% in 100 wt% of the total composition. The method according to claim 1,
Wherein the panthenol and the saliva oil are mixed at a weight ratio of 0.1 to 10: 0.1 to 20. The method according to claim 1,
The cosmetic composition may be at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a cream, a massage cream, a nutrition cream, a moisture cream, a hand cream, a foundation, , A cleansing foam, a cleansing lotion, a cleansing cream, a body cream, a body lotion, a body oil, a body essence or a body cleanser. Panthenol, and saline oil. The composition for external application for skin moisturizing and skin damage recovery. 8. The method of claim 7,
Wherein the panthenol is D-Panthenol, D, L-Panthenol or a mixture thereof. 8. The method of claim 7,
Wherein the panthenol is contained in an amount of 1 to 10% by weight in 100% by weight of the total composition. 8. The method of claim 7,
Wherein the saliva oil is contained in an amount of 0.005 to 1% by weight in 100% by weight of the total composition. 8. The method of claim 7,
Wherein the panthenol and saliva oil are mixed at a weight ratio of 0.1 to 10: 0.1 to 20. 8. The method of claim 7,
The composition for external application for skin for moisturizing and restoring skin damage, wherein the composition for external application for skin has a preventive or ameliorative effect on dry skin, atopic dermatitis, contact dermatitis or psoriasis. 8. The method of claim 7,
The composition for external application for skin may be an ointment, plasters, lotions, liniments, emulsions, aerosols, extracts, fludextracts, gels, hydrogels, suspensions, emulsions Wherein the composition is formulated into a paste, a paste, a spray, a patch, and a paste.

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