KR20170136474A - Cosmetic compositon for moisturizing comprising rehmannia glutinosa, astragalus membranaceus, angelica gigas and scutellaria baicalensis extract as an active ingredient - Google Patents

Abstract

The present invention provides a cosmetic composition for moisturizing comprising extracts of Rehmannia glutinosa, Astragalus propinquus, Angelica gigas, and Scutellaria baicalensis as active ingredients. The cosmetic composition for moisturizing according to the present invention comprises extracts of Rehmannia glutinosa, Astragalus propinquus, Angelica gigas, and Scutellaria baicalensis as active ingredients, so that when applied to skin, gene expression of hyaluronic acid synthase is increased, and the production of hyaluronic acid is promoted to reduce skin pulling, roughness, lack of elasticity and the like, thereby being effectively used for moisturizing the skin.

Description

[0001] The present invention relates to a cosmetic composition for moisturizing, comprising as an active ingredient, an extract of Aspergillus oryzae, Angelica keiskei, Angelica keiskei, Angelica gigas and Scutellaria baicalensis extract as an active ingredient.

The present invention relates to a cosmetic composition for moisturizing which comprises Rhodiola, Hwanggi, Angelica gigas and Golden extract as an active ingredient.

Recently, the tendency to develop cosmetics has been expanded and developed to emphasize functional aspects such as wrinkle improvement, whitening, and pore contraction beyond the previous cosmetic category. In particular, recently, the development of cosmetics for the purpose of alleviating the symptoms of sensitive skin or dry skin has been proceeding.

Moisturization of the skin is closely related to the moisture content of the stratum corneum on the outermost part of the epidermis. To maintain a healthy skin condition, the stratum corneum should contain about 30% moisture.

Hyaluronic acid (HA) is a kind of Glycosaminoglycan, which is a kind of Glycosaminoglycan. It has strong hydrophilic properties with collagen and elastin and forms a gel by binding with water. It is known as a natural moisturizing agent.

The concentration of HA in the skin is reduced by environmental factors such as age increases, long-term exposure to ultraviolet light, and the like. Such reduction of HA is known to cause reduction of elasticity, coarse skin and wrinkles as well as reduction of skin moisture content. Therefore, it is known that a substance that induces synthesis of HA or inhibits degradation is effective for skin moisturization and anti-aging, and HAS2 and HAS3 among hyaluronic acid synthase (HAS) plays a crucial role in synthesis of hyaluronic acid.

Therefore, it is necessary to study cosmetics that can increase the gene expression of HAS when applied to the skin and thereby promote the production of HA.

Korean Patent Publication No. 10-2010-0088881 (published on Aug. 11, 2010) Korean Patent Publication No. 10-2015-0007530 (published on January 21, 2015). Korean Registered Patent No. 10-1171369 (Registered on Jul. 31, 2012) Korean Registered Patent No. 10-1269993 (Registered on May 31, 2013)

The inventors of the present invention have found that when applied to skin, it is possible to increase gene expression by HAS, thereby promoting the production of hyaluronic acid and thus having a useful effect for skin moisturizing.

Accordingly, it is an object of the present invention to provide a cosmetic composition for moisturizing, which comprises Rhodiola, Hwanggi, Angelica gigas and Golden extract as an active ingredient.

The present invention provides a cosmetic composition for moisturizing, comprising as an active ingredient, Rhodiola, Hwanggi, Angelica gigas and Golden extract.

In one embodiment, the extract is characterized by 0.1 to 10% by weight based on the total weight of the composition.

In one embodiment, the extract is characterized by 1 to 5% by weight based on the total weight of the composition.

In one embodiment, the Rhizophora, Huanggi, Angelica and Golden extracts are characterized in that they are contained in equal proportions.

In one embodiment, the extract is characterized by extraction, concentration under reduced pressure or lyophilization.

In one embodiment, the formulation of the cosmetic composition is any one selected from the group consisting of lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) and water-in-oil (W / O).

In one embodiment, the composition further comprises at least one additive selected from the group consisting of purified water, oils, surfactants, moisturizers, chelating agents, pigments, fatty acids, antioxidants, waxes, flavors, preservatives and neutralizing agents .

The present invention provides a moisturizing cosmetic composition comprising the cosmetic composition described above.

The cosmetic composition for moisturizing according to the present invention contains hyaluronic acid, hwanggi, angelica, and golden extract as an active ingredient. When applied to skin, it increases gene expression of hyaluronic acid synthase, thereby promoting the production of hyaluronic acid, , Roughness, lack of elasticity and the like can be improved, so that it can be effectively used for moisturizing the skin.

FIG. 1 is an image showing the results of RT-PCR analysis of HaCaT cells treated with the extract of Example 1 (note that 1 represents 100 bp Ladder, 2 represents 0.1% DMSO-HAS2, 3 represents 1 μM ATRA-HAS2, 6 was the same as in Example 1 except that the extract of Example 1 was used in the same concentration of 50 / / ml-HAS2 for 3 times, 7 was 0.1% DMSO-GAPDH, 8 was 1 袖 M ATRA-GAPDH, ㎍ / ㎖-GAPDH, and 12 is 100bp Ladder).
FIG. 2 is a graph showing skin moisturizing measurement results of a ball region to which a skin prepared by the method according to Embodiment 2 is applied.
3 is a graph showing skin moisturizing measurement results of a ball region to which an essence prepared by the method according to Example 3 is applied.
FIG. 4 is a graph showing skin moisturizing measurement results of a ball region to which cream prepared by the method according to Example 4 is applied. FIG.

Hereinafter, the present invention will be described in detail.

The present invention provides a cosmetic composition for moisturizing, comprising as an active ingredient, Rhodiola, Hwanggi, Angelica gigas and Golden extract.

Gwanghwang is a plant belonging to perennial herbaceous ginseng and is rich in physiologically active substances such as catalpol, aucubin, etc., and is known to help improve immunity.

In addition, Hwanggi is a perennial herb belonging to soybean, and Hwanggi contains isoflavonoid and water-soluble vitamin, choline, which are known to help manage healthy skin.

Angelica is a herbal medicine of the buttercups, and the dexacin ingredient contained in Angelica gigas protects skin from active oxygen by activating skin metabolism and has been known to have a conditioning effect.

In addition, gold is a perennial herbaceous plant of the genus Lepidoptera, and is used as a herbal natural preservative, mainly for removing harmful oxygen, anti-inflammatory, antibacterial, antiallergic, antioxidant, whitening, anti-aging and moisturizing.

The hyaluronic acid, hyaluronic acid, hyaluronic acid, and gold are used as medicinal plants to induce gene expression of hyaluronic acid synthetase (HAS) upon application to skin, thereby increasing the production of hyaluronic acid (HA) .

The extract can be prepared using a commonly used hot water extraction method, solvent extraction method, backwash extraction method, etc., and a solvent extraction method can be preferably used.

The solvent used in the solvent extraction method may be various solvents such as purified water, alcohol, aqueous alcohol solution, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane or hexane. Preferably, can do.

For example, it has been found that application of Rhodiola, Huanggi, Angelica gigantosa and Golden extract to human keratinocyte, HaCaT, is effective in improving skin moisturization.

When the extract is contained in an amount of less than 0.1% by weight based on the total weight of the composition, it is difficult to expect the moisturizing effect of the extract to be sought in the present invention because its content is too small. When the extract is contained in an amount exceeding 10% by weight, And 0.1 to 5% by weight, and most preferably 0.5 to 1% by weight, based on the total weight of the composition.

In addition, in order to obtain a synergistic effect, the Rhizophora, Rhodochrosite, Angelica giganta, and Golden extract may be contained in the same ratio.

When the concentration of the extract is less than 10 μg / ml, it is difficult to expect a moisturizing effect by the extract. When the concentration of the extract is less than 200 μg / ml The cytotoxicity may be increased and the extract may contain the above-mentioned concentration range, more preferably the concentration may be 50 μg / ml.

Further, the extract may be constituted so as to be subjected to extraction under reduced pressure or lyophilized. At this time, the reduced-pressure concentrated or lyophilized extract may preferably be one dissolved in at least one solvent selected from purified water, ethanol, butylene glycol and propylene glycol.

The formulation of the cosmetic composition may be any one selected from conventional lotion, gel, water-soluble liquid, cream, essence, oil-in-water type and w / o type.

The cosmetic composition may further contain additives such as purified water, oil, a surfactant, a moisturizer, a chelating agent, a pigment, a fatty acid, an antioxidant, a wax, a flavor, a preservative, a neutralizing agent or a mixture thereof.

The cosmetic composition as described above may be formulated in the form of lotion, skin or cream by mixing with glycerin, thickeners, etc. Specifically, it may be formulated as a lotion, nutritional cream, massage cream, essence, eye essence, Eye patch or the like.

The composition of each of these formulations may contain various kinds of bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.

The present invention provides a moisturizing cosmetic composition comprising the cosmetic composition described above.

The moisturizing cosmetic composition containing the cosmetic composition according to the present invention may be used in accordance with a conventional method of use, and may be used in a number of times depending on the skin condition or taste of the user.

The cosmetic composition for moisturizing according to the present invention contains hyaluronic acid, hwanggi, angelica, and golden extract as an active ingredient. When applied to skin, it increases gene expression of hyaluronic acid synthase, thereby promoting the production of hyaluronic acid, , Roughness, lack of elasticity and the like can be improved, so that it can be effectively used for moisturizing the skin.

Hereinafter, the present invention will be described in more detail with reference to preferred embodiments. The embodiments presented are only a concrete example of the present invention and are not provided for the purpose of limiting the scope of the present invention.

Example 1. Preparation of Rhizophora, Hwanggi, Angelica and Golden extracts

Hwanggi, Hwanggi, Angelica and Golden were purchased from Puremind Pharmaceuticals (Gyeongbuk, Yeongcheon). The samples were stored in the Natural Products Laboratory of Sangmyung University Oriental Biotechnology Convergence Science Department. 100 g of each of the four kinds of medicines were measured and mixed, and the mixture was added to 2,000 ml of 70% (v / v) ethanol solvent. After cooling, the mixture was filtered and collected in a bottle. . The extract was concentrated by using a vacuum concentrator and then freeze-dried.

Example 2: Preparation of skins containing ginseng, hwanggi, angelica and golden extracts

A skin containing the extract prepared by the method according to Example 1 was prepared with the composition shown in Table 1 below.

ingredient Content (% by weight) Butylene glycol 7.00 glycerin 5.00 Hydroxyethanol 3.00 ethanol 1.50 Betaine 1.50 Page-32 1.00 Phenoxyethanol 0.30 Clofenine 0.20 Allantoin 0.20 Panthenol 0.20 Horse riding extract 0.20 Ginger extract 0.20 True Angelica extract 0.20 Golden extract 0.20 Hwanggi extract 0.20 Carbomer 0.20 PIGGY-40 Hydrogenated Castor Oil 0.20 Phi-Fi-26-Butes-26 0.20 Arginine 0.10 Tromethamine 0.10 Glyceryl polyacrylate 0.10 Xanthan gum 0.06 Dipotassium glycyrrhizate 0.05 Disodium iodide 0.02 Ethylhexyl Glycerin 0.02 Purified water Suitable amount Total amount 100.00

Example 3: Preparation of essence containing ginseng, hwanggi, angelica and golden extract

Essences containing the extract according to Example 1 were prepared with the compositions shown in Table 2 below.

ingredient Content (% by weight) Dipropylene glycol 6.00 Cetyl hexanoate 3.00 Caprylic / capric triglyceride 2.00 Hydrogenated coconut oil 2.00 Glyceryl stearate 2.00 ethanol 1.50 Cyclopentasiloxane 1.50 Glyceryl glucoside 1.50 Betaine 1.00 Benzyl glycol 0.92 Cyclohexasiloxane 0.50 Dimethicone 0.50 Sorbitan olivate 0.50 Cetearyl alcohol 0.20 Carbomer 0.20 Horse riding extract 0.20 Ginger extract 0.20 True Angelica extract 0.20 Golden extract 0.20 Hwanggi extract 0.20 Tromethamine 0.10 Arginine 0.10 Allantoin 0.10 Tocopheryl acetate 0.10 Panthenol 0.10 Butoxydiglycol 0.05 Raspberry ketone 0.03 Disease is DTI 0.02 Sodium hyaluronate 0.01 Propanediol 0.01 glycerin 0.01 Hydrolyzed deglycosaminoglycans 0.01 Purified water Suitable amount Total amount 100.00

Example 4. Manufacture of Creams Containing Ringer, Huangji, Angelica and Golden Extracts

Creams containing the extract according to Example 1 were prepared with the compositions shown in Table 3 below.

ingredient Content (% by weight) Cetyl hexanoate 7.00 Butylene glycol 7.00 glycerin 3.00 Glyceryl stearate S 2.00 Cetearyl alcohol 2.00 Hydrogenated coconut oil 2.00 Hydroxyethanol 2.00 Cyclopentasiloxane 2.00 Betaine 1.50 ethanol 1.50 Cyclohexasiloxane 1.50 Glyceryl stearate 1.00 FAGE -100 stearate 1.00 Benzyl glycol 0.92 Dimethicone 0.50 Beads wax 0.50 Carbomer 0.20 Horse riding extract 0.20 Ginger extract 0.20 True Angelica extract 0.20 Golden extract 0.20 Hwanggi extract 0.20 Tromethamine 0.10 Arginine 0.10 Sodium polyacrylate 0.10 Xanthan gum 0.10 Tocopheryl acetate 0.10 Panthenol 0.10 Butoxydiglycol 0.05 Allantoin 0.05 Raspberry ketone 0.03 Disodium iodide 0.02 Sodium hyaluronate 0.01 Purified water Suitable amount Total amount 100.00

Test Example 1. Analysis of extract

(1) Cytotoxicity analysis

To determine the cytotoxicity of the extract of Example 1, toxicity tests using MTT assay were performed.

The HaCaT cell line used for the toxicity test was purchased from ATCC, USA, and HaCaT cells were cultured in a cell culture medium (Dulbecco's Modified Eagle Medium, DMEM, Gibco) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin- And cultured in medium containing penicillin-streptomycin (Gibco) at 37 ° C and 5% CO ₂ .

First, the cells were seeded in a 96-well plate at a concentration of 2 x 10 ⁵ cells / ml. After 24 hours, they were replaced with serum-free DMEM and treated with the extracts of Example 1 at 10, 50, 100 and 200 / / ml, respectively. After 24 hours of incubation the medium was removed and 20 μl of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-hydrogen-tetrazolium bromide [3- 5-dimethyl-2-thiazolyl) -2,5-diphenyl-2H-tetrazolium bromide, MTT] was added and the cells were incubated for 2 hours in a CO ₂ incubator. After dissolving the crystals with 100 μl of dimethylsulfoxide (DMSO), the absorbance was measured at 570 nm and the final concentration of DMSO was adjusted to 0.1%.

When the cell viability was 100% when treated with 0.1 μM all-trans-retinoic acid (ATRA, Sigma), the cell viability was 92.65% when treated with 10 μg / ml of the extract of Example 1 , 93.73% when treated with 50 μg / ml, 94.02% when treated with 100 μg / ml, and 100.19% when treated with 200 μg / ml, respectively, showing no cytotoxicity upon treatment with the extract according to Example 1 .

(2) RT-PCR (reverse transcription-polymerase chain reaction) analysis

RT-PCR was used to confirm whether the extract of Example 1 expresses HAS2 (Hyaluronic acid synthase 2) gene.

First, HaCaT cells were placed in 6 well plates at a concentration of 2 x 10 < ⁵ > cells / ml. After 24 hours, serum-free DMEM was replaced and the extract of Example 1 was treated with 50 μg / ml. The final DMSO concentration was adjusted to 0.1%. After incubation for 24 hours, RNA was extracted with easyBlue (Intron). After measuring the RNA concentration and purity (OD 260 / OD 280), cDNA was synthesized with 2 μg RNA using a power cDNA synthesis kit (Intron). PCR was performed using a premix PCR kit (Solgent). The PCR product was electrophoresed on 1.5% agarose gel and the band was confirmed and shown in Fig. 1 μM of all-trans-retinoic acid (ATRA, Sigma) was used as a positive control.

The sequences of the primers used for reference are shown in Table 4 below.

gene direction The base sequence (5 '- > 3') Size (bp) HAS2 Forward GCT ACC AGT TTA TCC AAA CG (20 mer) 393 Reverse GTG ACT CAT CTG TCT CAC CG (20 mer) GAPDH Forward ATT GTT GCC ATC AAT GAC CC (20 mer) 546 Reverse AGT AGA GGC AGG GAT GAT GT (20 mer)

The PCR conditions were 94 ° C for 15 minutes, 32 to 35 cycles (94 ° C for 30 seconds, 50 ° C for 30 seconds, 72 ° C for 60 seconds, and 72 ° C for 10 minutes).

As shown in FIG. 1, the HAS2 gene was expressed in the extract at a concentration of 50 μg / ml, indicating that HA synthesis can be increased.

(3) HA enzyme-linked immunosorbent assay (HA-ELISA) assay

HA-ELISA (HA-ELISA kit, echelon) was performed to examine the change in the amount of HA produced when the extract according to Example 1 was treated.

First, HaCaT cells were placed in 6 well plates at a concentration of 2 x 10 < ⁵ > cells / ml. After 24 hours, the cells were washed twice with serum-free DMEM, fresh serum-free DMEM was then replaced, and the extract according to Example 1 was treated with 50 μg / ml. DMSO was adjusted to 0.1%. After 24 hours of extract treatment, 350 μl of the medium was centrifuged at 15,000 × g for 5 minutes, and the supernatant was collected and stored at 20 ° C. until ELISA was performed. Table 5 shows the HA yields measured at 0, 15, 30 and 45 minutes by ELISA using ATRA 1 [mu] M as a positive control.

Production amount (占 퐂 / ml) 0 minutes 15 minutes 30 minutes 45 minutes ATRA (30 minutes) Example 1
50 [mu] g / ml 517.025 528.904 543.324 516.959 759.091

As shown in Table 5, when the extract of Example 1 was treated at 50 占 퐂 / ml, the amount of HA produced was 543.32 占 퐂 / ml, which was the highest at the time of 30 minutes measurement. Thus, it can be seen that the extract according to Example 1 can express HA by expressing the HAS gene.

Therefore, the extract according to the example of the present invention was confirmed to exhibit the HA-producing effect as a safe natural product without toxicity, though it is not similar to the compound, like the compound.

Test Example 2. Skin moisturizing improvement effect Human body application test

(1) Skin

In order to analyze the skin moisturizing effect of skins containing Rhizophora, Huanggi, Angelica gigas, and Golden extract according to Example 2, it was applied to 22 female (average 40.636 years) face balls.

First, the face was washed with water and then waited for 30 minutes under constant temperature and humidity conditions. Then, the moisture content of the test site was measured with a moisture meter (Corneometer CM825, Courage and Khazaka, Germany) before application of the sample, 2 weeks after application of the sample, ). The results are shown in Table 2 below, and skin moisturizing growth rate is shown in Table 7. Statistical analysis was performed using the IBM SPSS statistics version 21.0 program.

Before application After 2 weeks of application After 4 weeks of application Average (A.U) 55.007 61.678 64.704 Standard Deviation 6.862 7.158 7.262

After 2 weeks of application After 4 weeks of application Growth rate (%) 12.612 18.457

As shown in Table 6, Table 7 and FIG. 2, the skin moisturization increase rate was 12.612% and 18.457% after 2 weeks and 4 weeks, respectively, at a statistically significant level compared with before application of the sample. Thus, it can be seen that the skins containing Rhododendron, Rhodochrosophila, Angelica gigas, and Golden extract according to Example 2 are effective for increasing skin moisturization.

(2) essence

In order to analyze the skin moisturizing effect of the essence including Rhizophora, Huanggi, Angelica gigas and Golden extract according to Example 3, it was applied to 22 female (average 43.272 years old) face balls.

First, the face was washed with water, and then waited for 30 minutes under constant temperature and humidity conditions. Then, the moisture content of the test area was measured three times using a moisture meter before the application of the sample, two weeks after the application of the sample, The results are shown in Table 8 and FIG. 3, and skin moisturizing growth rates are shown in Table 9.

Before application After 2 weeks of application After 4 weeks of application Average (A.U) 52.096 64.467 70.672 Standard Deviation 8.028 6.771 5.237

After 2 weeks of application After 4 weeks of application Growth rate (%) 25.177 38.091

As shown in Table 8, Table 9, and FIG. 3, the skin moisturizing growth rate was 25.177% and 38.091% after 2 weeks and 4 weeks, respectively, at a statistically significant level compared to that before the application of the sample. Thus, it can be seen that the essence containing the extract of Gujung, Huanggi, Angelica gigas and Golden extract according to Example 3 is effective for increasing skin moisturization.

(3) Cream

In order to analyze the skin moisturizing effect of the cream containing ginseng, hwanggi, angelica, and golden extract according to Example 4, it was applied to 22 female (average 45.045 years old) face balls.

First, the face was washed with water, and then waited for 30 minutes under constant temperature and humidity conditions. Then, the moisture content of the test area was measured three times using a moisture meter before the application of the sample, two weeks after the application of the sample, Shown in Table 10 and FIG. 4, and skin moisturizing growth rate is shown in Table 11.

Before application After 2 weeks of application After 4 weeks of application Average (A.U) 59.960 64.184 67.883 Standard Deviation 8,354 7.725 7.832

After 2 weeks of application After 4 weeks of application Growth rate (%) 7.733 14.026

As shown in Table 10, Table 11, and FIG. 4, the skin moisture increase rate was 7.733% and 14.026% at 2 weeks and 4 weeks after application, respectively, at a statistically significant level compared to before application of the sample. Thus, it can be seen that the cream containing Zyghe, Huanggi, Angelica gigas and Golden extract according to Example 4 helps increase skin moisturization.

Claims (6)

Wherein the composition comprises 0.1 to 5% by weight of ethanol extract of Chinese cabbage, Chinese cabbage, angelica, golden, and golden, based on the total weight of the composition. The moisturizing cosmetic composition according to claim 1, further comprising an ethanol extract of horse riding. The cosmetic composition for moisturizing according to claim 1, wherein the extract is extracted and concentrated under reduced pressure or freeze-dried. The cosmetic composition according to claim 1, wherein the formulation of the cosmetic composition is any one selected from the group consisting of lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) A cosmetic composition for moisturizing. The composition according to claim 1, further comprising at least one additive selected from the group consisting of purified water, an oil, a surfactant, a moisturizer, a chelating agent, a pigment, a fatty acid, an antioxidant, a wax, a flavoring agent, Wherein the cosmetic composition is a moisturizing cosmetic composition. A moisturizing cosmetic comprising the moisturizing cosmetic composition according to any one of claims 1 to 5.

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