KR20170067380A - Cosmetic compositions containing Desmodii Herba extaracts for prevention of skin aging and improvement of skin wrinkle - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging and improving skin wrinkles, and more particularly, to a cosmetic composition containing a ginseng extract, and further to a cosmetic composition containing a ginseng extract and a ginseng extract. The cosmetic composition of the present invention is excellent in anti-aging, skin wrinkle improvement, and skin condition improvement.

Description

[0001] The present invention relates to cosmetic compositions for preventing skin aging and improving skin wrinkles,

The present invention relates to a cosmetic composition for preventing skin aging and improving skin wrinkles, and more particularly, to a cosmetic composition containing a ginseng extract, and further to a cosmetic composition containing a ginseng extract and a ginseng extract.

As the largest organ of the human body, which occupies about 16% of the total human body volume, the skin is directly contacted with the external environment, and it is important to protect the human body from many harmful factors that intrude into the human body such as temperature, humidity and ultraviolet rays. It plays a protective role. However, with age, skin cells are damaged due to various pollutants, strong ultraviolet rays, stress and nutritional deficiency, cell proliferation is not properly performed, and wrinkles, elasticity loss, and keratinization occur in the skin.

Skin aging is largely divided into intrinsic aging and extrinsic aging (Cosmetics & Toiletries, 111: 31-37 (1996)). My human aging is characterized by a gradual decline in the physiological function of the skin as it ages,

(Arch Dermatol., 130: 87-95 (1994)), which is characterized by a clinically reduced elasticity, coarse skin texture, deep wrinkles and pigmentation. External aging refers to the aging phenomena caused by external factors such as ultraviolet rays, reactive oxygen species and stress. These factors lead to rapid aging of the skin, resulting in rough wrinkles on the skin surface and a significant reduction in elasticity.

Several studies have been carried out to elucidate the aging mechanism of skin and the causes of various skin aging have been revealed. When the skin is dried by a change in temperature, a decrease in humidity, or wind, the skin physiological activity as a barrier against the outside is lowered and aging is promoted. In addition, when skin is exposed to harmful active oxygen species or free radicals, lipid peroxidation is produced by the oxidative action in the body, which causes deformation of various proteins constituting the skin. On the other hand, collagen and elastic elastin, which occupy most of the skin dermis (about 70-80% of the total dry weight of the skin), are the main proteins produced by fibroblasts, and are involved in mechanical durability of the skin, (Journal of the american academy of dermatology, 21: 610-613 (1989)). Collagen shows a quantitative decrease with increasing age and exposure to ultraviolet light. Collagen is classified according to its morphological and structural characteristics, among which the most well characterized are types I, II, III and IV. Collagen types I and III are the

And collagen type IV is a major component of DEJ (Dermal Epidermal Junction). DEJ is located between the epidermis and the dermis of the skin to control the permeation and transport of the material between the two layers. It regulates the differentiation of adjacent skin cells and maintains the firmness of the skin. Collagen and elastin, the main structural components of the skin, are denatured or biosynthetic by natural or external stimuli, which accelerates skin aging.

In order to inhibit or slow down the progress of skin aging, cosmetics or skin protectants containing various skin protecting ingredients such as retinol, retinoid, vitamin C, flavonoid and tocopherol are being developed. However, such a cosmetic or skin protecting agent has a weak potency in the final formulation and has a slight effect on skin irritation.

Because the skin has many opportunities to interact with various external stimuli, it is more prone to wrinkles than other organs. Among them, facial skin is directly exposed to sunlight, dry outside air and pollutants, so that aging phenomenon such as wrinkles occurs more early than other skin. The most characteristic change due to aging of the skin tissue is a change in the skin matrix, which causes aging of the human skin fibroblast in the dermis, resulting in a decrease in the ability to produce fibrous and matrix. The amount of the substrate is generally decreased, the thickness of the skin is thinned, and the elasticity of the skin is lowered to form the wrinkles. That is, as aging progresses, the skin becomes weaker in elasticity, disorder of blood circulation, weakening of skin barrier, and the like. In addition, exposure of the skin to ultraviolet rays produces free radicals and reactive oxygen species, the main causes of skin cell damage. They can cause damage to the DNA, attack cell membrane structures, generate aging spots, and accelerate wrinkle formation by attacking collagen and fibers that keep skin moist, soft and supple and elastic.

Hwangjin Outpatient 9, Inhibitory effect of extracts of Fusarium chinense on breast cancer cells, Physiology of Oncology 28 (5) 494-498 (2014)

The present invention provides a cosmetic composition which can be used for preventing skin aging and improving skin wrinkles.

The present invention provides a cosmetic composition for preventing skin aging and improving skin wrinkles, which comprises an extract of Ganoderma lucidum as an active ingredient.

Further, the cosmetic composition is preferably a cosmetic composition for preventing skin aging and improving skin wrinkles, which further comprises a gold sphagnum extract as an active ingredient, wherein the mixture of the gold sphagnum and the gold nuggets is solvent extracted.

Further, it is preferable to further include a pineapple extract as an active ingredient, wherein a mixture of the pineapple and the pineapple leaf is solvent-extracted.

In addition, it is preferable that the present invention further comprises a gold skein and a pine needles extract as an effective ingredient, and a solvent mixture of a mixture of a gold microspheres, a gold skein and a pine needles.

Further, the extract is preferably a cosmetic composition characterized by being an extract which is soluble in water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

In the present invention, it is possible to additionally include gold scales and ginseng extracts based on the extract of Fusarium chinense.

Desmodii Herba is a pre-plant of the two-plant Desmodium styracifolium, and its medicinal area is above the ground. The stem is cylindrical and has a length of about 1m. It is yellowish, covered with dense, soft hair, and the vagina is slightly tight. Leaves are horny, lobules are 1 ~ 3, round to round square, 2 ~ 4cm in diameter, the front end is slightly concave and the base is heart-like to gentle circle. The upper surface is yellowish green to grayish green with no hairs, and the lower surface has villi firmly attached to grayish white color. The taste is sweet and sour, and the quality is cool. Ingredients are Alkaloid, Flavonoid glycoside, etc., and are known to have efficacy in urinary infection, urinary stone, nephritis, cholecystitis, lacquer, pediatric marker, hematoma, jaundice. However, the antioxidative effect and the improvement effect of the skin quality of the fungus are not known at present. Disclosure of the Invention The present invention has found a possibility as a cosmetic composition for a fungus, and completed the present invention.

Rosa laevigata is the fruit of the Rosaceae. It is obovate with fruit, length is 2 ~ 3.5cm, diameter is 1 ~ 2cm. The outer surface is yellowish red to reddish brown, and the traces of the thorns are convex to a small point of brown. There is a tray-shaped calyx in the upper part, yellow pedicel remains in the center, and the lower part is gradually pointed. Quality is hard. On the cut face, calyx is 0.1 ~ 0.2cm in wall thickness, and it is hard inside and contains several small aqueducts. The angel is strong in the function of converging all the leaks, so the body is fragile, and the hacho is poor, and it is used for oil wells, urine, frequent urination. It is also used for sweating, sweating, sweating, diarrhea, diarrhea, uterine bleeding, coughing and shortness of breath.

The 杜 沖 葉 is the leaf of the mulberry tree. Leaves are elliptical or long ovate, 7 ~ 10cm long, 4 ~ 6cm wide. The upper surface is gray to dark green with no hair, and the lower surface is green gray. Its tip is pointed, its bottom is dull, it has sawtooth on the edge, and petiole. The vagina is easy to break, and the tongue will increase the thread-like rubber. There is a peculiar smell. The quality is warm and the taste is a bit spicy. It has the effect of seeing forgiveness, strengthening muscles and lowering blood pressure. Pain in the back and back, feet and knees weak and weak, when used for hypertension. It is known that it contains chlorogenic acid, caffeic acid, aubucine, gum, glycans such as glycosides, flavonoids, alkaloids, organic acids and the like.

As used herein, the term "extract " means an active ingredient isolated from a natural product. The extract can be obtained by an extraction process using water, an organic solvent, or a mixed solvent thereof, and includes an extract, a dry powder thereof, or all the forms formulated with it. In the present invention, the extract of Pseudomonas fluorescens can be extracted by using water, an organic solvent or a mixed solvent thereof. Preferably a lower alcohol having 1 to 4 carbon atoms, or an extract obtained by using the mixture in a mixed solvent thereof. Especially, ethanol is preferably used for extraction. The extracted liquid can be used directly or by concentrating and / or drying. In the case of extraction using an organic solvent, an organic solvent such as methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF) (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof. The active ingredient of the herbal medicine may be extracted at room temperature or warmed under the condition that the active ingredient is not destroyed or minimized. Depending on the organic solvent to be extracted, the degree of extraction and the degree of loss of the active ingredient of the medicament may differ. Therefore, an appropriate organic solvent should be selected and used. The extraction method is not particularly limited, and examples thereof include cold extraction, ultrasonic extraction, and reflux cooling extraction. Filtration is a process of removing suspended solid particles from an extract, and may be performed by filtering particles using a cotton, nylon, or the like, or by ultrafiltration, freezing filtration, centrifugation, and the like. Concentration of the extract can be carried out by reduced-pressure concentration, reverse osmosis concentration, or the like. The post-concentration drying step includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying and the like. In some cases, a step of pulverizing the final dried extract may be added. In addition, the extract may be subjected to an additional fractionation process.

In order to examine whether the extract of the present invention has an anti-aging effect and an improvement in skin wrinkles, it is necessary to determine whether there is an antioxidative effect, an inhibitory effect on collagenase expression, an inhibitory effect on elastase activity, And the promotion of cell differentiation. The present inventors again tested the effect of skin elasticity, skin wrinkle and skin moisturizing effect through the actual cosmetic formulation using the extract of the present invention. As a result, the extracts of Gwangmaekcho extract showed some excellent effects, and the extracts of ginseng extract, mixed extract of ginseng leaves, and ginseng extracts of Gwanggeumgwa, ginseng, and ginseng showed a synergistic effect. It is more effective to mix each substance and solvent extraction together rather than mixing each extract. When each material is mixed, 50 to 200 parts by weight of the added material may be mixed based on 100 parts by weight of the optical brightener. More preferably, the same amount of material is mixed and extracted.

The cosmetic composition of the present invention is excellent in anti-aging, skin wrinkle improvement, and skin condition improvement.

Hereinafter, the present invention will be described in more detail through examples and test examples. However, the embodiments and the like are used for illustrative purposes in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited thereto.

≪ Preparation of extracts of Gwanggimcheoncho &

3 ㎏ of Gwangmyeongcho purchased from Seoul Medicinal Plant was dried and pulverized for 5 days at shade and room temperature. The pulverized light pulverizer was immersed in 30 L of 95% ethanol and extracted at 50 DEG C for 24 hours. This was filtered through a filter paper, dried and concentrated under reduced pressure at 45 캜 to obtain 390 g of a total extract (Example 1).

≪ Preparation of gold scales,

Using 3 kg of gold skein purchased from Seoul Medicament Co., 490 g of total extract was obtained by the same method (Example 2).

In addition, 3 kg of pupae were extracted in the same manner to obtain 392 g of a total extract (Example 3).

≪ Preparation of mixture extract >

1.5 kg of the ginkgo simulator and 1.5 kg of the gold sperm were mixed and extracted by the same method to obtain 475 g of the total extract (Example 4).

1.5 kg of Fusarium monocytogenes and 1.5 kg of Pseudomonas sp. Were extracted and extracted in the same manner to obtain 473 g of a total extract (Example 5).

The mixture was mixed with 1 kg of each of green beans, gold persimmon, and green beans, and extracted by the same method to obtain 490 g of the total extract (Example 6)

≪ Test Example 1 >

In order to examine the antioxidative effect of the extract of the examples of the present invention, a free radical scavenging activity experiment was conducted. The antioxidant activity was evaluated by comparing DPPH antioxidant activity with that of free radical DPPH (1,1-diphenyl-2-picryl hydrazyl). Trolox, a synthetic antioxidant widely used as a positive control, was used.

190 μl of 100 μM (in ethanol) DPPH solution, 10 μl of each of the extracts of the example of the present invention and the positive control were added to the reaction solution, and reacted at 37 ° C for 30 minutes. Then, the absorbance was measured at 540 nm.

The IC50 of Trolox showed 47 ppm, and the IC50 of Example 1 showed 62, showing antioxidative effect equal to trolox when compared to Trolox. The IC50 values of Example 2 and Example 3 were 107 and 113, respectively. However, in Examples 4, 5 and 6, 51, 54, and 46 were shown, and the mixed extracts showed excellent antioxidative effects.

Test Example 2: Inhibitory effect on expression of collagenase

The ability of the extract of the above Example to inhibit collagenase expression was measured in comparison with EGCG. EGCG is an antioxidant that is known to regenerate epidermal cells of the skin and prevent aging of the skin.

Human fibroblasts were plated at 5,000 cells per well in a 96-well plate containing DMEM medium containing 2.5% urea serum and cultured to 80% growth. Example and EGCG were treated at a concentration of 20 占 퐂 / ml for 24 hours, and cell culture medium was collected. The degree of collagenase production of the cell culture broth was measured using a commercially available collagenase measuring device. First, the cell culture solution collected on a 96-well plate uniformly coated with collagenase antibody was added and the antigen-antibody reaction was performed in a thermostatic chamber for 3 hours. Subsequently, the second collagen antibody conjugated with chromophore was added to a 96-well plate, and color development was induced at room temperature for 15 minutes. Then, 1 M sulfuric acid was added to stop the color development reaction. The color is yellow, and the degree of yellowishness varies depending on the extent of the reaction. Absorbance was measured at 405 nm, and the absorbance of the treated group was divided by the absorbance of the untreated group and the degree of expression was examined. The lower the degree of expression of collagenase, the higher the ability to inhibit the expression of collagenase and the less collagen decomposition in the skin, the less elasticity of skin elasticity will be inhibited and the amount of wrinkles to be formed will be reduced.

When the degree of expression of the untreated group was taken as 100, EGCG was 75%, and in Example 1, 69%, effectively suppressing the expression of collagenase. In Examples 2 and 3, the inhibitory effect was not so good, but the mixed extracts of Examples 4, 5, and 6 were 66, 67, and 60, respectively.

<Test Example 3 - Elastase activity inhibitory effect>

The elastase activity inhibition of the Examples was measured in comparison with EGCG. Cannell RJP, Kellan SJ, Owsians AM, and Walker JM. Results of a large scale screening of microalgae for protease inhibitors. Planta Med. 1988; 54 (1): 10 -14). The amount of pnitroanilide produced from the substrate at 37 ° C for 20 minutes was measured at 445 nm using N-succinyl- (L-Ala) 3-p-nitroanilide as a substrate. 0.5 ml of each test solution was added to 0.5 ml of each test solution. 0.5 ml of porcine pancreas elastase (2.5 U / ml) dissolved in 50 mM tris-HCl buffer (pH 8.6) N-succinyl- (L-Ala) 3-p-nitroanilide (0.5 mg / ml) dissolved in HCl buffer (pH 8.6) was added for 20 minutes. Elastase inhibitory activity was determined in accordance with (absorbance of 1-treated group / absorbance of untreated group) * 100.

When the inhibition rate of the untreated group was regarded as 0%, the EGCG was 21% and the EGCG was 37% as compared with the EGCG. Examples 4, 5 and 6, which are mixed extracts, showed excellent characteristics as 48, 43 and 54%.

<Test Example 4 - Melanin formation inhibitory effect>

The melanogenesis inhibitory effect of the examples was measured relative to the known whitening substance hydroquinone. The cells were cultured in DMEM supplemented with 10% fetal bovine serum, 100 nM 12-O-Tetradecanoyl (10 nM) Acetate, and 1 nM cholera toxin in the presence of 5% CO2 at 37 &lt; 0 &gt; C. The cultured Mel-Ab cells were removed with 0.25% trypsin-EDTA, and the cells were cultured at a concentration of 100 cells / well in a 24-well plate. Then, each test substance was added for 3 consecutive days Respectively. As a test substance, hydroquinone and the example were used at a concentration of 10 占 퐂 / ml. Then, the culture solution was removed, washed with PBS, and then the cells were dissolved with 1 N sodium hydroxide, and the absorbance at 400 nm was measured. The absorbance was then calculated as 100 (absorbance of the 1-treated group / absorbance of the untreated group)

When the inhibition rate of melanin formation in the untreated group was taken as 100, the inhibition rate of hydroquinone was 43%, and Example 1 showed 38%, showing a relatively excellent effect. And 39% in the case of Example 2 as well. In the case of the mixed extract, Example 4 shows a synergistic effect by showing 47%, and Example 6 shows an additional synergistic effect by 56%.

&Lt; Test Example 5 - Promoting effect of keratinocyte differentiation &

In order to examine the differentiation promoting effect of keratinocytes, the amount of corned envelope (CE) generated upon differentiation of keratinocytes was measured by absorbance. First, the keratinized cells of a primary culture separated from the epidermis of a newborn baby are placed in a culture flask and adhered to the bottom. After the material of the example is treated at a concentration of 10 ppm in the culture medium, when the cells grow to about 80% of the floor area For 5 days. At this time, low calcium (0.03 mM) and high calcium (1.2 mM) treatment groups were negative control and positive control, respectively. Then, the cultured cells were harvested, washed with PBS (Phosphate buffered saline), and then suspended in 10 mM Tris-HCl buffer (Tris-HCl, pH 7.4) containing 2% SDS (sodium dodecyl sulfate) and 20 mM DTT (Dithiothreitol) pH 7.4) was added, sonication, boiling, and centrifugation. The precipitate was suspended again in 1 ml of PBS and the absorbance at 340 nm was measured. Separately, a part of the solution after the sonication was taken to measure the protein content and used as a standard in evaluating the degree of cell differentiation.

The positive control group was 205, the negative control group was 139, the negative control group was 132, the negative control group was 148, and the negative control group had a synergistic effect. Thus, 165, 161, and 174, respectively. In the case of bifidobacterium, it seems to promote the differentiation of keratinocytes.

Based on the above results, a cream formulation was prepared using Example 1, Examples 4, 5 and 6 to further test.

<Formulation Example>

The extracts of Example 1, 5, 7, polyglyceryl 2-olate 1.4, ceramide 0.6, cetearate -4.13, dicetyl phosphate 0.5, carlytriglyceride 10, stearyl alcohol 2, Nutrient cream was prepared in a composition of trace amount, triethanolamine 0.2, and purified water balance (unit weight%) (Formulation Example 1).

Formulations 2, 3 and 4 were prepared in the same manner as in Formulation Example 1, using the extracts of Examples 4, 5 and 6, respectively.

<Comparative Formulation Example>

In Formulation Example 1, a nutritional cream was prepared except for the extract of Example 1 (Comparative Formulation Example).

&Lt; Test Example 6 - skin elasticity >

In order to confirm the skin elasticity improvement effect in human, the above formulation examples and comparative formulations were evaluated as follows. Forty healthy 50 females were divided into 10 groups, and the nutritional cream was dispensed to each of the formulations and the comparative formulations, and applied to the left side of the face for 6 weeks, twice a day, morning and evening, followed by a skin elasticity tester (Cutometer SEM 575, C + K Electronic Co., Germany). The results show the properties of skin viscoelasticity.

And 0.18 for the comparative formulation, and Formulation Examples 1 to 4 showed the results of 0.33, 0.39, 0.41, and 0.54, respectively. It was found that the composition containing the extract of Gwangmyeongcho extract was effective for improving the skin elasticity.

&Lt; Test Example 7 - Efficacy of Skin Wrinkle Reduction >

The wrinkle-improving effect of the composition of the present invention on human was further evaluated for the object of Test Example 6 above. Silicone was used for image analysis by measuring the state of the wrinkles by measuring the state of the wrinkles with a skin meter (visometer, SV600, Courage + Khazaka electronic GmbH, Germany). And the value obtained by subtracting the pre-application variable value from the variable value after 6 weeks was averaged. The difference (R1) between the maximum value and the minimum value of the wrinkle contour line, the average value (R1) of the R1 values, the maximum value (R3) among the R1 values divided by 5, the angle between the baseline of the wrinkle contour line The mean value (R4) of the top and valley minus the value (R5) and the average value (R5) of the baseline of the wrinkle contour minus each wrinkle line are obtained.

In the case of the comparative formulations, there was no positive wrinkle improvement effect, and all of the formulations showed improvement in wrinkles. In particular, in the case of Formulation Example 4, the effect of improving the wrinkles of the skin was particularly excellent.

&Lt; Test Example 8 - Skin moisturizing effect >

In order to evaluate the effect of the extract of the present invention on the skin moisturizing effect, the skin moisturizing effect of the formulation examples and the comparative formulations was evaluated as follows. Fifty females of 50 females classified as dry skin were divided into 10 groups each, and the nutritional creams of Formulation Examples 1 to 4 and Comparative Formulation were applied to the face for 2 weeks each for 4 weeks. (24 ° C, relative humidity 40%) after 1 week, 2 weeks, and 4 weeks after application, and after 2 weeks (total 6 weeks) (Corneometer CM825, C + K Electronic Co., Germany). The increase in the measured value after treatment for a certain period based on the value of the skin moisture meter measured immediately before the start of the test was measured as a percentage.

In the case of the comparative formulation, the moisture increase rate was about 30% until the 4th week of application, but when the application was stopped, the skin moisture content decreased to about 15%. The formulations of the examples showed a water increase rate of 30% or more overall, and a water increase rate of about 40% at 4 weeks, and a water increase rate of 30% or more at 6 weeks of discontinuation of application, It was confirmed that the improvement effect of the moisturizing condition was continued. In particular, in Comparative Formulation Examples 3 and 4, the maintenance rate of the water increase rate after stopping application was about 35%.

From the above results, it was confirmed that the extract of the present invention has effects of preventing skin aging, improving wrinkles, promoting differentiation of keratinocytes, and increasing skin moisturizing ability through improvement of skin condition.

Claims (5)

A cosmetic composition for preventing skin aging and improving skin wrinkles comprising an extract of Ganoderma lucidum as an active ingredient. The method according to claim 1,
A cosmetic composition for preventing skin aging and improving skin wrinkles, which further comprises an extract of Angelica keiskei koidz. The method according to claim 1,
A cosmetic composition for preventing skin aging and improving skin wrinkles, which further comprises a ginseng leaf extract as an active ingredient, wherein a mixture of ginseng powder and ginseng leaves is solvent-extracted. The method according to claim 1,
A cosmetic composition for preventing skin aging and improving skin wrinkles, which further comprises a gold scales and a pineapple extract as an active ingredient, wherein a mixture of a scarlet pigment, a gold scarf and a pine needles is solvent extracted. 5. The method according to any one of claims 1 to 4,
Wherein the extract is an extract soluble in water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.