KR20170036849A - A method of disrupturing microalgae - Google Patents

A method of disrupturing microalgae Download PDF

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Publication number
KR20170036849A
KR20170036849A KR1020150132260A KR20150132260A KR20170036849A KR 20170036849 A KR20170036849 A KR 20170036849A KR 1020150132260 A KR1020150132260 A KR 1020150132260A KR 20150132260 A KR20150132260 A KR 20150132260A KR 20170036849 A KR20170036849 A KR 20170036849A
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South Korea
Prior art keywords
microalgae
oxide
titanium oxide
calcium oxide
crushing
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KR1020150132260A
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Korean (ko)
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김종덕
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김종덕
중소기업은행
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Priority to KR1020150132260A priority Critical patent/KR20170036849A/en
Publication of KR20170036849A publication Critical patent/KR20170036849A/en

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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/72—Treatment of water, waste water, or sewage by oxidation
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • C02F1/5236—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F5/00—Softening water; Preventing scale; Adding scale preventatives or scale removers to water, e.g. adding sequestering agents
    • C02F5/02—Softening water by precipitation of the hardness
    • C02F5/06—Softening water by precipitation of the hardness using calcium compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/10—Energy recovery
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/26—Reducing the size of particles, liquid droplets or bubbles, e.g. by crushing, grinding, spraying, creation of microbubbles or nanobubbles

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Organic Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

More particularly, the present invention relates to a composition for crushing microalgae containing titanium oxide (TiO 2 ) and calcium oxide (CaO) as an active ingredient, and a method for crushing microalgae using the composition .

Description

A method of disrupting microalgae

The present invention relates to a composition for crushing microalgae and a method for crushing microalgae.

In the summer, drought and inflow of contaminants cause a phenomenon of overgrowth of microalgae including green algae in major rivers, causing serious problems such as the cause of fish and the difficulty of using them as drinking water in the vicinity of the water source · It is becoming an economic problem. In order to deal with these green algae, the local governments are spreading them using water cannons mainly in the areas with severe green algae, or are carrying out the level of control activities using yellow loam. Even if these microalgae are collected, the collected microalgae are burned as organic wastes and buried.

However, green algae contains a large amount of carbon and lipid components, and when it is recycled, bioethanol and biodiesel can be produced, and by-products such as green algae produced during the production of these biofuels can be used as feed for livestock .

Therefore, in order to produce biofuels such as bioethanol using these microalgae, it is essential to develop a pretreatment process that economically recovers and crushes microalgae. In connection with the disruption of double microalgae, mechanical destruction methods using bead mills (Chisti, Y., Moo-Young, M., Enzyme Microb. Technol., 8: 194-204, 1986) (Wang et al., Int. J. MoI. Sci., 16 (4): 169-178, 1983) 7702-7722, 2015), chemical detergents using chemicals (Brown and Audet, JR Soc. Interface 5 (Suppl 2): S131-S138, 2008) (Kim et al., Bioresource Technol., 102: 3163-3168, 2011), using a chemical such as aCl2 and FeCl2, 10, 1998), herb disruption using herb extracts (Yang et al., Int. J. Mol. Sci., 10 9): 4157-4167, 2009) and a catalyst destruction method using a catalyst containing TiO2 (Shen et al., Water Sci. Technol., 70 (5): 828-834, 2 014).

However, the mechanical crushing method may not be efficient depending on the kind of the microalgae. In the case of the enzyme crushing method, excessive cost is a problem. In the case of the catalyst crushing method, since the catalyst manufacturing process is complicated and expensive, There is a disadvantage that it is difficult.

Accordingly, the present inventors have made efforts to provide a more economical and efficient method for crushing microalgae and a method of crushing microalgae, and as a result, they have found that titanium oxide (TiO 2 ) and calcium oxide CaO) is treated with water containing microalgae, and various types of microalgae can be economically and efficiently disrupted, thereby completing the present invention.

An object of the present invention is to provide a composition for microalgae crushing using titanium oxide (TiO 2 ) and calcium oxide (CaO) at the same time, and a method for crushing microalgae from water containing microalgae using the composition.

In order to achieve the above object, the present invention provides a composition for crushing microalgae containing titanium oxide (TiO 2 ) and calcium oxide (CaO) as an effective ingredient.

The present invention also provides a composition for crushing microalgae containing titanium oxide (TiO 2 ), calcium oxide (CaO) and magnesium oxide (MgO) as active ingredients.

The present invention also provides a method for disintegrating microalgae comprising treating titanium oxide (TiO 2 ) and calcium oxide (CaO) with water containing microalgae.

In addition, the present invention provides a method for disintegrating microalgae comprising treating titanium oxide (TiO 2 ), calcium oxide (CaO), and magnesium oxide (MgO) with water containing microalgae.

When a mixture of titanium oxide and calcium oxide or titanium oxide, calcium oxide and magnesium oxide according to the present invention is treated in microalgae, useful components in the microalgae can be extracted without any further treatment. The method according to the present invention can economically and efficiently break down microalgae and can be very useful for extracting and purifying useful components such as biodiesel and bioethanol from microalgae.

FIG. 1 is a photograph of a state in which AC current (AC) is applied to water containing microalgae according to an embodiment of the present invention and the state becomes a liquid phase phase separation according to the passage of time.
2a-c are micrographs of microscopic algae breakdown of microalgae according to the time course of treatment of microalgae according to an embodiment of the present invention.
2A is a photomicrograph of a change in the shape of microalgae of Comparative Examples 1 to 3 (when only TiO 2 was treated).
2B is a photomicrograph showing changes in the appearance of microalgae of Comparative Examples 4 to 6 (when only CaO and MgO were treated)
FIG. 2C is a photomicrograph of a change in the shape of microalgae during the treatment of the compositions of Examples 1 to 6 of the present invention. FIG.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for crushing microalgae containing titanium oxide (TiO 2 ) and calcium oxide (CaO) as an active ingredient.

The composition may comprise 0.5 to 2 parts by weight of calcium oxide per part by weight of titanium oxide.

The composition may further comprise magnesium oxide (MgO), wherein the composition may include from 0.5 to 2 parts by weight of calcium oxide and from 0.5 to 2 parts by weight of magnesium oxide, based on 1 part by weight of titanium oxide.

The microalgae may be selected from the group consisting of Cyanobacteria, Cryptophyta, Chrysophyta, Euglenophyta, Bacillariophyta, Phaeophyta, Rholophyta, Chlorophyta, Charophyta), or a mixture of two or more thereof.

The green alga may be selected from the group consisting of Chlamydomonas sp., Botryococcus sp., Schizochytrium sp., Scenedesmus sp., Prymnesium sp. ), Amphidinium sp.), nose Ella Sturm in (Coelastrum sp.), tetra-cell misses in (Tetraselmis sp.), tetracycline seutiseu in (Tetracystis sp.), protocol chiffon in (Protosiphion sp.), hydrochloride Dick thione in (Hydrodictyon sp.) Phedi mast room in (Pediastrum sp.), chloro kokum in (Chlorococcum sp.), wool matrix in (Ulothrix sp.), O, even in Konishi Titanium (Oedogonium sp.), chlorella genus (chlorella sp.), styryl to Eau Ronnie help in (Stigeoclonium sp.), Fritz sh Ella in (Fritschiella sp.), spiro rep in (Spirogyra sp.), jig nematic in (Zygnema sp.) or climb FIG Fora in (Cladophora sp.) one .

The cyanobacterium belongs to the genus Microcystis sp., Nostoc sp. Tolypothrix sp., Aulosira sp., Anabaena sp., Planktothrix sp., Cylindrospermum sp., Spodoptera sp. Fischerella sp., Gloeotrichia sp., Nodularia sp., Oscillatoria sp., Aphanizomenos sp. Aphanizomenon sp., Lyngbya sp.), rapiyi aid in sheath (Rhaphidiopsis sp.), chestnut source Forum in (Chrysosporum sp.) Syracuse Titus matrix in (Cuspidothrix sp.), genus Synecoccus sp.), Cylindrospermopsis sp., Dolichospermum sp., Phormidium sp., Tychonema sp., Woroninichna sp. Woronichinia sp.) Or Arthrospira sp.

The present invention also provides a method for disintegrating microalgae comprising treating microalgae with titanium oxide (TiO 2 ) and calcium oxide (CaO) in water containing microalgae.

In the above method, the microalgae may be green algae, cyanobacteria, axon algae, euglena, brown algae, sulfur brown algae, sulfur algae or red algae.

In the above method, the water containing the microalgae may be microalgae-contaminated water, microalgae culture solution, or recovered microalgal slurry.

In the above method, the green algae are selected from the group consisting of Chlamydomonas sp., Botryococcus sp., Schizochytrium sp., Scenedesmus sp. in (Prymnesium sp.), cancer nium in PD (Amphidinium sp.), nose Ella Sturm in (Coelastrum sp.), tetra-cell misses in (Tetraselmis sp.), tetracycline seutiseu in (Tetracystis sp.), protocol chiffon in (Protosiphion sp.), hydrochloride Dick thione in (Hydrodictyon sp.) Phedi mast room in (Pediastrum sp.), chloro kokum in (Chlorococcum sp.), wool matrix in (Ulothrix sp.), O, even in Konishi Titanium (Oedogonium sp.), chlorella genus (chlorella sp.), styryl to Eau Ronnie help in (Stigeoclonium sp.), Fritz sh Ella in (Fritschiella sp.), spiro rep in (Spirogyra sp.), jig nematic in (Zygnema sp.) or climb FIG Fora in (Cladophora sp.) one .

In the above method, the cyanobacterial is selected from the group consisting of Microcystis sp., Nostoc sp. Tolypothrix sp., Aulosira sp., Anabaena sp., Planktothrix sp., Cylindrospermum sp., Spodoptera sp. Fischerella sp., Gloeotrichia sp., Nodularia sp., Oscillatoria sp., Aphanizomenos sp. Aphanizomenon sp., Lyngbya sp.), rapiyi aid in sheath (Rhaphidiopsis sp.), chestnut source Forum in (Chrysosporum sp.) Syracuse Titus matrix in (Cuspidothrix sp.), genus Synecoccus sp.), Cylindrospermopsis sp., Dolichospermum sp., Phormidium sp., Tychonema sp., Woroninichna sp. Woronichinia sp.) Or Arthrospira sp.

In the above method, the water containing microalgae may be microalgae-contaminated water, microalgae culture solution, or recovered microagal slurry. The recovered microalgae slurry may be obtained by recovering suspended microalgae by applying alternating current to the water or microalgae culture fluid contaminated with microalgae.

In the above method, the titanium oxide and the calcium oxide can be treated in an amount of 50 to 1,000 mg, respectively, per 1 L of water containing microalgae.

In this method, further magnesium oxide can be treated, in which case the titanium oxide, calcium oxide and magnesium oxide can each be treated with 50 to 1,000 mg per 1 L of water containing microalgae.

In the process, a mixture of titanium oxide and calcium oxide, or titanium oxide, calcium oxide and magnesium oxide, may be processed sequentially, either simultaneously or sequentially.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples.

However, it should be understood that the present invention is not limited to the embodiments and examples described below, but may be embodied in various other forms. The following embodiments and examples are intended to be illustrative, It is provided to fully inform the owner of the scope of the invention.

< Example  And Comparative Example > Preparation of composition for microalgae crushing

The present inventors prepared compositions for microalgae crushing with the compositions shown in Table 1 below.

The components and the throughput of the composition for microalgae crushing of the present invention ingredient TiO 2 (% w / v) CaO (% w / v) MgO (% w / v) Comparative Example 1 0.01 - - Comparative Example 2 0.03 - - Comparative Example 3 0.05 - - Comparative Example 4 - 0.01 0.01 Comparative Example 5 - 0.03 0.03 Comparative Example 6 - 0.05 0.05 Example 1 0.01 0.01 - Example 2 0.03 0.03 - Example 3 0.05 0.05 - Example 4 0.01 0.01 0.01 Example 5 0.03 0.03 0.03 Example 6 0.05 0.05 0.05

< Experimental Example  1> Pretreatment of cultured microalgae

Prior to carrying out the microalgae crushing experiment, the recovery of microalgae in culture was carried out using the following method.

The microalgae used for the experiment are the cyanobacteria Microcystis aeruginos ). First, a phase change was observed for 5 minutes while applying an electric current of 200 to 240 V for household use to 5 L of microcystis aeruginosa culture solution. In the application of the household AC, the plug was inserted into the household AC outlet without using the special power supply, and the exposed wire was inserted into the beaker containing the culture liquid. At this time, In order to prevent this problem, a partition wall is installed in the beaker and both wires are inserted between the partition walls.

As a result, the microalgae agglomerated on the upper part of the beaker and the lower culture became gradually transparent (FIG. 1). Therefore, it was confirmed that the AC current application method is a very useful method for selectively collecting microalgae from water containing microalgae. The water below the floating flocculated microalgae was sucked and removed by the pump, and the remaining microalgae were collected and used for the subsequent microalgae crushing experiment.

< Experimental Example  2> Fragmentation of microalgae

The microalgae recovered at the time of Experiment 1 were treated with the compositions for crushing microalgae prepared in the above Comparative Examples and Examples, and the degree of microalgae crushing was checked over time.

As a result, as shown in FIG. 2A, it was confirmed that the microalgae were hardly dissolved even when the concentration of titanium oxide was increased during the single treatment of titanium oxide as the comparative example.

In addition, as shown in FIG. 2B, when only calcium oxide and magnesium oxide were treated, partial cell destruction occurred only after 120 minutes of 0.05% CaO and 0.05% MgO treatment. And it was found.

On the other hand, as shown in FIG. 2C, when the titanium oxide and calcium oxide were treated (Examples 1 to 3) or titanium oxide, calcium oxide and magnesium oxide were mixed (Examples 4 to 6) It was confirmed that the dissolution of the microalgae was further promoted with increasing time and with increasing time. In addition, it was confirmed that the microbial destruction occurred more efficiently when the three components of titanium oxide, calcium oxide and magnesium oxide were mixed than titanium oxide and calcium oxide mixture.

Therefore, when a mixture of titanium oxide and calcium oxide or a mixture of titanium oxide, calcium oxide and magnesium oxide according to an embodiment of the present invention is processed into microalgae, microalgae can be used to extract the useful components in the microalgae without any further treatment. Can be broken.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. I will understand. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

When the composition for crushing microalgae according to the present invention as described above is used, the microalgae can be economically and efficiently crushed, and is very useful for extracting and purifying useful components such as biodiesel and bioethanol from crushed microalgae Lt; / RTI &gt;

Claims (13)

A composition for crushing microalgae containing titanium oxide (TiO 2 ) and calcium oxide (CaO) as an effective ingredient.
The method according to claim 1,
And 0.5 to 2 parts by weight of calcium oxide based on 1 part by weight of the titanium oxide.
The method according to claim 1,
A composition for microalgae crushing, further comprising magnesium oxide (MgO).
The method of claim 3,
0.5 to 2 parts by weight of calcium oxide and 0.5 to 2 parts by weight of magnesium oxide per 1 part by weight of the titanium oxide.
Titanium oxide (TiO 2) and calcium oxide crushing method of the micro-algae which comprises (CaO) microalgae (microalgae) in the treated water contains.
6. The method of claim 5,
The microalgae may be selected from the group consisting of Cyanobacteria, Cryptophyta, Chrysophyta, Euglenophyta, Bacillariophyta, Phaeophyta, Rholophyta, Chlorophyta, Charophyta), or a mixture of two or more thereof.
The method according to claim 6,
The green alga may be selected from the group consisting of Chlamydomonas sp., Botryococcus sp., Schizochytrium sp., Scenedesmus sp., Prymnesium sp. ), Amphidinium sp.), nose Ella Sturm in (Coelastrum sp.), tetra-cell misses in (Tetraselmis sp.), tetracycline seutiseu in (Tetracystis sp.), protocol chiffon in (Protosiphion sp.), hydrochloride Dick thione in (Hydrodictyon sp.) Phedi mast room in (Pediastrum sp.), chloro kokum in (Chlorococcum sp.), wool matrix in (Ulothrix sp.), O, even in Konishi Titanium (Oedogonium sp.), chlorella genus (chlorella sp.), styryl to Eau Ronnie to help in (Stigeoclonium sp.), Fritz sh Ella in (Fritschiella sp.), spiro rep in (Spirogyra sp.), jig nematic in (Zygnema sp.) and Cloud FIG Fora in (Cladophora sp.) Lt; RTI ID = 0.0 &gt; alga, &lt; / RTI &gt;
The method according to claim 6,
The cyanobacterium belongs to the genus Microcystis sp., Nostoc sp. Tolypothrix sp., Aulosira sp., Anabaena sp., Planktothrix sp., Cylindrospermum sp., Spodoptera sp. Fischerella sp., Gloeotrichia sp., Nodularia sp., Oscillatoria sp., Aphanizomenos sp. Aphanizomenon sp., Lyngbya sp.), rapiyi aid in sheath (Rhaphidiopsis sp.), chestnut source Forum in (Chrysosporum sp.) Syracuse Titus matrix in (Cuspidothrix sp.), genus Synecoccus sp.), Cylindrospermopsis sp., Dolichospermum sp., Phormidium sp., Tychonema sp., Woroninichna sp. Woronichinia sp., And Arthrospira sp.).
6. The method of claim 5,
Wherein the water containing microalgae is water, microalgae culture medium, or recovered microalgal slurry contaminated with microalgae.
10. The method of claim 9,
Wherein the recovered microalgae slurry is obtained by recovering suspended microalgae by applying an alternating current to a water or microalgae culture fluid contaminated with microalgae.
6. The method of claim 5,
Wherein the titanium oxide and calcium oxide are each treated with 50 to 1,000 mg per 1 L of water containing microalgae.
6. The method of claim 5,
Wherein the magnesium oxide is additionally treated.
13. The method of claim 12,
Wherein the titanium oxide, calcium oxide and magnesium oxide are each treated with 50 to 1,000 mg per 1 L of water containing microalgae.
KR1020150132260A 2015-09-18 2015-09-18 A method of disrupturing microalgae KR20170036849A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019182181A1 (en) * 2018-03-22 2019-09-26 새론아쿠아 주식회사 Method for removing microalgae by using rotifers
KR20200058862A (en) * 2018-11-20 2020-05-28 중앙대학교 산학협력단 Cultivating method of microalgae using titanium dioxide nano particles

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019182181A1 (en) * 2018-03-22 2019-09-26 새론아쿠아 주식회사 Method for removing microalgae by using rotifers
KR20200058862A (en) * 2018-11-20 2020-05-28 중앙대학교 산학협력단 Cultivating method of microalgae using titanium dioxide nano particles

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