KR20170032623A - Chionanthus retusa extract and cosmetic composition comprising the extract - Google Patents

Abstract

Provided are a Chionanthus retusus Lindl. & Paxton extract for antioxidation, skin anti-aging, whitening, or moisturization, and a cosmetic composition containing the same. By containing a natural extract derived from plants as an active ingredient, the cosmetic composition is eco-friendly, and ensures excellent 2,2-diphenyl-1-picryhydrazyl radical (DPPH) reactive oxygen scavenging effects, collagen biosynthesis-promoting effects, effects of inhibiting collagenase expression, effects of inhibiting melanin production, and effects of promoting keratinocyte differentiation, thereby exhibiting outstanding antioxidative effects, skin anti-aging effects, whitening effects, or moisturizing effects.

Description

[0001] The present invention relates to an extract of Cinnamomum cassia L., and a cosmetic composition containing the extract,

More particularly, the present invention relates to an extract of Euphorbiaceae having an antioxidative effect, a skin anti-aging effect, a whitening effect or a moisturizing effect using a natural component and a cosmetic composition containing the same will be.

Active oxygen is known to cause cellular diseases such as aging or cancer by destroying cellular components such as lipids, proteins, sugars and DNA. In addition, the body peroxide produced as a result of lipid oxidation by the active oxygen also causes oxidative destruction to cells, resulting in various functional disorders. Accordingly, antioxidants such as free radical scavengers or peroxidation-inhibiting substances are expected as therapeutic agents for various diseases.

Skin aging is a phenomenon in which collagen and elastin, the major components of the skin, are denatured and the elasticity of the skin is lost. Expression of matrix metalloproteases such as collagenase and elastase degrades collagen and elastin, resulting in reduced collagen and elastin content in the skin. (Dermatology Therapy, 1998, 16, 357-364), retinoids (Leguminosae Seeds), and retinoids have been used for the purpose of inhibiting collagen and elastin degradation. Fraction is also applied to show the effect of increasing the elasticity (U.S. Patent No. 5,322,839). However, the retinoid has a disadvantage in that irritation occurs even when only a small amount of the retinoid is applied to the skin.

There are many factors involved in determining human skin color, among which melanin, the most important factor in determining human skin color. The melanin pigment is a phenolic polymer substance having a complex form of a black pigment and a protein. It protects skin organs below the dermis by blocking ultraviolet rays irradiated from the sun, and at the same time, catches free radicals generated in the skin of the skin, It plays a useful role in protecting genes.

However, melanin, which is produced by external stress stimuli, is a stable substance that does not disappear until the skin is excreted through skin keratinization even if the stress disappears. Therefore, when melanin is produced more than necessary, it induces hypercholesterolemia such as spots, freckles, and dots, resulting in poor cosmetic results. In addition, as the number of people enjoying outdoor activities increased due to the increase in the leisure population, the demand for preventing melanin pigmentation due to ultraviolet rays increased. In response to such demands, substances having inhibitory activity against ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione or tyrosinase have been used in cosmetics or medicines. However, inadequate whitening effect, , And the use thereof is limited due to the problem of formulation and stability in combination with cosmetics.

One aspect of the present invention is to provide an extract of a natural plant having an antioxidative effect, a skin aging-preventing effect, a whitening effect, or a moisturizing effect.

Another aspect of the present invention is to provide a cosmetic composition comprising an extract of a natural plant having an antioxidant effect, a skin aging-preventing effect, a whitening effect, or a moisturizing effect.

According to one embodiment of the present invention, an extract of Chionanthus retusa for antioxidation, skin aging prevention, whitening or moisturizing is provided.

The extract may be extracted from one or more selected from the group consisting of leaves, petals, stems, bark, fruits, seeds and sap.

According to another embodiment of the present invention, there is provided a cosmetic composition comprising an extract of Euphorbiaceae as an active ingredient.

The extract may be at least one selected from the group consisting of leaf, petal, stem, bark, fruit, seed and sap.

The poppy tree extract may be contained in an amount of 0.0001 to 50% by weight based on the weight of the whole composition.

The composition may be formulated as a softening agent, a nutritional lotion, a massage cream, a nutritional cream, a pack, a gel, a skin sticking type, a transdermal administration type, a lotion, an ointment, a gel, a cream, a patch or a spray.

The composition may further comprise at least one selected from the group consisting of a preservative, a moisturizer, an emollient, a surfactant, an organic pigment, an inorganic pigment, an organic powder, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a plant extract, a pH adjuster, An antiseptic agent, a cold agent, an antiperspirant agent, and purified water.

The use of natural extracts derived from plants as an active ingredient has natural, but excellent DPPH radical scavenging effect, collagen biosynthesis promoting effect, collagenase expression inhibiting effect, melanin formation inhibiting effect and keratinocyte differentiation promoting effect, Thus, it is possible to exhibit excellent antioxidative effect, skin aging prevention effect, whitening effect or moisturizing effect.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. However, the embodiments of the present invention can be modified into various other forms, and the scope of the present invention is not limited to the embodiments described below.

According to one embodiment of the present invention, it is possible to provide an antioxidant, an anti-aging skin, a whitening agent or a moisturizing antler seed extract (Chionanthus retusa).

Chionanthus retusa, the active ingredient of the present invention, is about 20 m in height, and the bark is ash-toned brown, with slightly hairs on the branches, with petioles facing each other, long petiole, oval, 3 ~ ~ 6cm. The leaf is flat but the leaf of young shoot has double sawtooth, the surface is green, the back is light green, and the vein has light brown hairs. Flowers are dioecious, bloomed in May-June, with conical flower buds at the end of branches. Calyxes and corolla are divided into 4, petals are white and butterflies are about 3mm long. The peduncle is 7 ~ 10mm long and has nodes. There are two stamens attached to a flower basket and one pistil. The fruit is oval, dark purple, ripening in October to November, and breeding is possible with seeds or bunches.

The extract is preferably extracted from at least one selected from the group consisting of leaves, petals, stems, bark, fruits, seeds and sap, and is more preferably extracted from sap.

The extract of the present invention can be extracted by a suitable method known in the art. For example, an organic solvent is added to the leaves, petals, stems, bark, fruits, or seeds of dried poppy, After 1 day of immersion at 15 ° C, the residue and filtrate are separated by filtration and centrifugation through a filter cloth, and the separated filtrate is concentrated under reduced pressure to obtain leaf, petal, stem, bark, The sap is extracted and dried by spraying to obtain an extract of Sapporo japonicus.

The organic solvent is not particularly limited and may be a C ₁ to C ₅ lower alcohol, ether, ethyl acetate or chloroform. The C ₁ to C ₅ lower alcohols may be used for mixing one or more kinds selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol .

According to another embodiment of the present invention, it is possible to provide a cosmetic composition comprising an extract of Euphorbiaceae as an active ingredient. As can be seen in the following examples, the cosmetic composition of the present invention has an excellent DPPH active oxygen eliminating effect, collagen biosynthesis promoting effect, collagenase expression inhibiting effect, melanin formation inhibiting effect and keratinocyte differentiation promoting effect, , Excellent antioxidative effect, skin aging prevention effect, whitening effect or moisturizing effect can be exhibited.

It is preferable that the ginseng extract contained in the composition is extracted from at least one selected from the group consisting of leaves, petals, stems, bark, fruits, seeds and sap, more preferably extracted from sap.

The extract is preferably contained in an amount of 0.0001 to 50% by weight, more preferably 1 to 20% by weight based on the weight of the whole composition. If the content of the extract is less than 0.0001% by weight based on the weight of the whole composition, the antioxidative effect, the anti-aging effect, the whitening effect or the moisturizing effect of the extract of Echinoderm are not sufficient. There is a fear of denaturation of cosmetics, etc., and it is difficult to control the viscosity of cosmetic formulations and it is difficult to develop cosmetic formulations because production of emulsion formulations is easy to take place.

The cosmetic composition of the present invention contains the above-mentioned ingredients as an active ingredient and may be prepared by appropriately blending ingredients other than the active ingredient according to various desired formulations.

More specifically, the cosmetic composition according to the present invention can be prepared in any form conventionally produced in the art. For example, the composition can be used as a softening agent, a nutritional lotion, a massage cream, a nutritional cream, a pack, Skin-adhesive type, transdermal type, lotion, ointment, gel, cream, patch or spray formulations.

In addition, the composition of the present invention may be combined with the components contained in a general cosmetic composition, if necessary, together with the extract of Echinacea. The blending components contained in the composition may be selected from the group consisting of a preservative component, a moisturizer, an emollient, a surfactant, an organic pigment, an inorganic pigment, an organic powder, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a plant extract, A blood circulation accelerator, a cold agent, an antiperspirant agent, and purified water.

Hereinafter, the present invention will be described more specifically by way of specific examples. The following examples are provided to aid understanding of the present invention, and the scope of the present invention is not limited thereto.

Example

1. Manufacture of apple tree extract

[Example 1]

The extract was refluxed three times with 4 L of 80% methanol in 500 g of dried leafy leaves, and then immersed at 15 캜 for 1 day. Thereafter, the extract was filtered using a filter cloth, and the residue and the filtrate were separated by centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain an extract.

[Example 2]

The extract was obtained in the same manner as in Example 1 except that the nip tree fruit was used instead of the euphorbiaceae leaf.

 [Example 3]

The extract was obtained in the same manner as in Example 1, except that an apple tree trunk was used instead of the lobster.

 [Example 4]

The extract was obtained in the same manner as in Example 1 except that the bark bark was used instead of the bark.

[Example 5]

10 L of the sap obtained from the poppy tree was spray-dried with hot air at 80 캜 to obtain an extract.

2. Antioxidant effect test (DPPH test)

The antioxidative effects of the extracts obtained from Examples 1 to 5 were measured by DPPH (1,1-diphenyl-2-picryl hydrazyl) active oxygen scavenging test.

The DPPH free radical scavenging test is a method of evaluating the sulfur oxidizing ability through the change of the absorbance generated by the reduction of the free radical DPPH. 10 μl each of the test substances listed in Table 1 below was added to 190 μl of 100 μM (in ethanol) DPPH solution to make a reaction solution, reacted at 37 ° C. for 30 minutes, and the absorbance was measured at 540 nm. At this time, Trolox was used as a control sample. The DPPH analysis results of Examples 1 to 5 and Trolox are shown in Table 1 below, wherein IC50 is a sample concentration when the absorbance is reduced by 50% by the added sample.

sample IC50 (ppm) Example 1 75 Example 2 64 Example 3 45 Example 4 65 Example 5 35 Trolox 65

As can be seen in Table 1 above, Examples 1-5 inhibit the oxidation of organic radicals at similar or lower concentrations than Trolox used as an antioxidant. Accordingly, it was confirmed that the extract of Echinacea according to the present invention has an excellent antioxidative effect.

3. Anti-aging effect test - Promoting collagen biosynthesis

The anti-aging effects of the extracts obtained from Examples 1 to 5 were measured by a collagen biosynthesis accelerating effect test.

Human fibroblasts (PromoCell, Germany) were seeded at 10 ⁵ per well in 24 wells and cultured until they grew to about 90%. The cells were cultured in a serum-free DMEM (Dulbecco's modified Eagle's medium) for 24 hours, and then the test substances in the following Table 2 were treated at 10 ^-4 mol / L in the serum-free medium and cultured in a carbon dioxide incubator for 24 hours. At this time, tocopherol and EGCG (Epigallocatechin-3-gallate) were used as positive control samples. The supernatant of the cultured medium was lifted and the procolagen (I) ELISA kit (proc / ollagen type (I)) was used to measure the increase or decrease of procollagen. The analytical results of Examples 1 to 5, tocopherol and EGCG are shown in Table 2 below, wherein the combined performance (%) is obtained by setting the untreated group to 100%.

sample Sum performance (%) Example 1 148 Example 2 155 Example 3 160 Example 4 145 Example 5 140 Tocopherol 118 EGCG 125 Untreated group 100

As can be seen from the above Table 2, Examples 1 to 5 are superior to the positive control groups of tocopherol and EGCG in promoting collagen biosynthesis. Accordingly, it has been confirmed that the extract of Echinosophora koreensis according to the present invention effectively promotes collagen biosynthesis, thereby having an excellent anti-aging effect on skin.

4. Anti-aging effect test - Measurement of inhibitory effect on collagenase expression

The anti-aging effects of the extracts obtained from Examples 1 to 5 were measured by a collagenase inhibitory effect test.

Human fibroblasts were plated at 5,000 cells / well in a 96-well microtiter plate containing DMEM medium containing 2.5% fetal bovine serum, and cultured to a growth of about 90% . Then, the cells were cultured in a serum-free DMEM medium for 24 hours, treated with the test substances shown in the following Table 3 at a concentration of 10 ^-4 mol for 24 hours in the serum-free DMEM medium, and then the cell culture medium was collected. At this time, tocopherol and EGCG were used as positive control samples.

Antibody-antibody reaction was performed in a thermostatic chamber for 3 hours by adding the cell culture medium collected in a 96-well plate uniformly coated with primary collagenase antibody. After 3 hours, the second collagen antibody bound to the chromophore was placed in a 96-well plate and reacted for another 15 minutes. After 15 minutes, the color development inducing substance was added to induce color development at room temperature for 15 minutes, and 1M sulfuric acid was added to stop the reaction (color development). At this time, the color of the reaction solution was yellow and the degree of yellow was different according to the progress of the reaction. The absorbance of a yellow 96-well plate was measured at 405 nm using a spectrophotometer (Amersham Pharmacia, USA) and the degree of expression of collagenase was calculated by the following equation , Where the reaction absorbance of the untreated group was determined as the absorbance of the control group.

[Mathematical Expression]

Collagenase expression level (%) = A / B X 100

A: absorbance of the cell group treated with the test substance

B: Absorbance of the control group

The degree of collagenase expression of Examples 1 to 5, tocopherol and EGCG is shown in Table 3 below, wherein the degree of collagenase expression (%) was prepared by setting the degree of collagenase expression in the untreated group to 100% .

sample Expression level of collagenase (%) Example 1 30 Example 2 36 Example 3 46 Example 4 44 Example 5 26 Tocopherol 75 EGCG 60 Untreated group 100

As can be seen in Table 3, Examples 1 to 5 are superior to collagenase expression inhibiting effect than tocopherol and EGCG, which are positive control groups. Therefore, it was found that the extract of Echinochloa crus-galli according to the present invention effectively inhibited the expression of collagenase, thereby having excellent skin aging-preventing ability.

5. Whitening effect test

The whitening effect tests on the extracts obtained from Examples 1 to 5 were performed by a test for inhibiting melanin production using mouse pigment cells.

(Dooley, TP et al, Skin pharmacol, 7, pp 188-200) derived from C57BL / 6 mice were cultured in DMEM medium at 37 ° C under 5% carbon dioxide. The DMEM medium is a medium supplemented with 10% fetal bovine serum, 100 nM 2-O-tetradecanoyphorbol-13-acetate, and 1 nM cholera toxin. The Mel-Ab cells cultured in the DMEM medium were removed with 0.25% trypsin-EDTA, and the cells were cultured at a concentration of 10 ⁵ cells / well in a 24-well plate. Thereafter, the test substances of Table 4 were added at 10 ppm each for 3 consecutive days from the second day, followed by incubation. At this time, hydroquinone was used as a positive control. At the end of the incubation, the culture medium was removed, washed with PBS, and the cells were lysed with 1 N sodium hydroxide to measure the absorbance at 400 nm. The inhibition rate (%) of melanin production was calculated by the following equation (3) Absorbance of the control group. The calculation results are shown in Table 4 (Dooley's method).

&Quot; (3) "

sample Melanin production inhibition rate (%) Example 1 68 Example 2 59 Example 3 58 Example 4 67 Example 5 74 Hydroquinone 53 Untreated group 0

As can be seen from the above Table 4, Examples 1 to 5 are superior to hydroquinone in the effect of inhibiting melanin formation. Thus, it has been confirmed that the extract of Echinacea according to the present invention effectively inhibits the production of melanin, thereby having an excellent skin whitening effect.

6. Moisturizing effect test

Moisturizing effect tests on the extracts obtained in Examples 1 to 5 were measured by a test for promoting keratinocyte differentiation. Specifically, the amount of CE (cornified envelope) produced upon differentiation of keratinocytes was measured using absorbance Respectively.

The keratinized cells of the primary cultures isolated from the epidermis of the newborn infants were placed in a culture flask and adhered to the bottom. The test substances in the following Table 5 were each treated at a concentration of 1 ppm in the culture medium, % ≪ / RTI > until growth. At this time, low calcium (0.03 mM) and high calcium (1.2 mM) treatment groups were used as negative control or positive control, respectively. The cultured cells were harvested and washed with PBS (Phosphate Buffered Saline). Then, the cells were washed with 10 mM Tris-HCl buffer (Tris-HCl, pH (1)) containing 2% SDS (sodium dodecyl sulfate) and 20 mM DTT (Dithiothreitol) 7.4) was added to perform sonication, and then boiling was performed. The precipitate separated by centrifugation was suspended again in 1 ml of PBS and absorbance was measured at 340 nm. Separately, a part of the solution after the sonication was taken to measure the protein content and used as a standard for assessing the degree of cell differentiation. The results are shown in Table 5 below.

Test substance The ability to differentiate in keratinocytes (%) Low calcium (0.03 mM) solution (negative control) 100 High calcium (1.2 mM) solution (positive control) 210 Example 1 146 Example 2 175 Example 3 142 Example 4 131 Example 5 126

As shown in Table 5, it was confirmed that Examples 1 to 5 promoted differentiation in keratinocytes. Therefore, it has been confirmed that the extract of Echinacea according to the present invention has enhanced skin barrier function and skin moisturizing effect.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, It will be obvious to those of ordinary skill in the art.

Claims (7)

Antioxidant, anti-aging, whitening or moisturizing extract of Chionanthus retusa.
The method according to claim 1,
Wherein the extract is extracted from at least one selected from the group consisting of leaf, petal, stem, bark, fruit, seed and sap.
A cosmetic composition comprising an extract of Euphorbiaceae as an active ingredient.
The method of claim 3,
The cosmetic composition as described above is obtained from at least one selected from the group consisting of leaves, petals, stems, bark, fruits, seeds and sap.
The method of claim 3,
The cosmetic composition according to claim 1, wherein the extract is from 0.0001 to 50% by weight based on the weight of the total composition.
The method of claim 3,
Wherein the composition is formulated into a formulation of a softening agent, a nutritional lotion, a massage cream, a nutritional cream, a pack, a gel, a skin adhesive type, a transdermal type, a lotion, an ointment, a gel, a cream, a patch or a spray.
The method of claim 3,
The composition may further comprise at least one selected from the group consisting of a preservative, a moisturizer, an emollient, a surfactant, an organic pigment, an inorganic pigment, an organic powder, an ultraviolet absorbent, an antiseptic, a bactericide, an antioxidant, a plant extract, a pH adjuster, Wherein the cosmetic composition further comprises at least one selected from the group consisting of cold agents, antiperspirants and purified water.