KR20160146372A - Cosmetic composition with the extract of Rehmannia glutinosa, Scutellaria baicalensis and Isatis indigotica against skin aging - Google Patents

Abstract

The present invention relates to a cosmetic composition which contains at least one extract from the group consisting of a Rehmannia glutinosa extract, a Scutellaria baicalensis extract, and an Isatis indigotica extract. The extract of the present invention suppresses the generation of various factors related to the aging of skin and promotes the activity of various factors related to antiaging, and thereby the cosmetic composition containing the extract of the present invention exhibits an excellent antiaging effect.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition for preventing aging of skin comprising extracts of Chinese cabbage, Chinese cabbage extract, golden extract and extract of Daechung (Cosmetic composition with extract of Rehmannia glutinosa, Scutellaria baicalensis and Isatis indigotica against skin aging)

The invention is Rehmannia (Rehmannia it relates to a cosmetic composition for preventing skin aging, more particularly glutinosa extract, golden ( Scutellaria baicalensis extract, Isatis indigotica extract of the present invention.

Skin aging can be divided into endogenous aging which finds the cause of aging from the inside and extrinsic aging which finds the cause from the outside. Endogenous skin aging is a natural aging phenomenon that occurs over time and is known to act as a direct or indirect cause of active oxygen. Exogenous skin aging has been reported to cause skin cancer, including thick and deep wrinkles, reduced skin elasticity, and pigmentation, due to aging caused by various external stresses including UV rays.

Exogenous skin aging is also known as photoaging because most of its causes are sunburn. The clinical features of photoaging are more severe than endogenous aging and show aging early on. The photoaging is mainly characterized by abnormal findings of the dermis, and the decrease of the collagen fiber and the accumulation of the elastic fiber material are remarkable.

On the other hand, global warming means global warming of the air around the sea and on the surface. From 1906 to 2005, the world average surface temperature rose 0.8 degrees Celsius. However, the rate of warming accelerated to the point where the warming rate in the latter half of this period accounted for more than half, and according to the IPCC, it is expected that there will be a temperature rise of about 2 to 4.5 degrees Celsius in 2100. This means that Australia, which has an average temperature of 36 to 38 degrees Celsius, will rise to 40 to 42 degrees Celsius. In recent years, interest in thermal aging including infrared rays as well as photoaging due to ultraviolet rays has been increasing, and heat generated in daily life such as use of a steam room, a sauna, a computer or a mobile phone, and a hair dryer has also become a main cause of heat aging .

The ideal temperature of the skin is 30 to 31 degrees Celsius lower than body temperature. People at normal temperatures will experience skin temperatures up to 43 degrees Celsius for 30 minutes when exposed to intense sunlight. When the skin temperature rises, the enzymes that break down collagen, which is the main constituent of the skin, are activated to promote skin damage and aging, which leads to wrinkles. It has also been reported that oxidative stress, which is increased by heat, is involved in skin aging.

Meanwhile, Rehmannia glutinosa ) is a ginseng and plant, and its roots are coarse, stretched to the side, and reddish brown. It is a medicinal plant of many years, and its roots are used as medicinal materials. In the oriental medicine, the raw materials of roots are called raw persimmon, dried persimmon leaves, steamed persimmon leaves and steamed persimmon leaves. It is used for blood sugar, diabetes and blood pressure drop. It is used for fever, detoxification, heartache, blood clots, nosebleeds, uterine bleeding and menstrual irregularities. Dried persimmon is used for thirst after fever and fever It is known to be effective.

Golden ( Scutellaria baicalensis ) is a herbaceous and perennial herbaceous plant that contains the physiologically active components of flavonoids and wogonin and has the effect of releasing heat of the heart and lungs or stopping the cough by pharmacological effect.

Isatis indigotica ) is a biennial plant belonging to the cruciferous family, and has been used as herbal medicine since ancient times. In one room, Daechung Leaf is used as Daechung Leaf, and Root is used as a plant name. It is used for the treatment of various infectious diseases such as cold, pneumonia, viral hepatitis, and it has the merit of acupuncture meridian and detoxification (antipyretic, anti-inflammatory, antibacterial action).

However, no description has been made on a cosmetic composition for preventing skin aging which contains an extract of a mixture composed of ginseng, golden, and ginseng.

Korean Patent No. 10-1010744 (Registration Date: Jan. 18, 2011) describes a technique for a composition for exfoliating, which contains a water extract of Chinese persimmon. Korean Patent No. 10-0761248 (registered on September 18, 2007) describes a technique for a composition for treating atopic dermatitis containing a mixed composition of bamboo extract and gold extract. Korean Patent Laid-Open No. 10-2008-0053851 (published on Jun. 16, 2008) discloses a technique for a cosmetic composition for treating and preventing acne containing a Panrum root extract.

The present invention relates to the use of Rehmannia glutinosa extract, golden ( Scutellaria baicalensis ) extract, Isatis indigotica extract of the present invention.

In order to achieve the above object, the present invention Rehmannia (Rehmannia glutinosa ), golden ( Scutellaria baicalensis ), and Taisho ( Isatis indigotica ) as an active ingredient. The present invention also provides a cosmetic composition for preventing skin aging, which comprises an extract of at least one member selected from the group consisting of indigotica . Chrysanthemum extract, golden extract and Daechung extract do not show toxicity to cells, so they are harmless to the human body and have no irritation and are suitable for skin cosmetics. It also increases the expression of heat shock proteins reduced by thermal stimulation, inhibits the development of factors associated with skin aging, and promotes the activity of factors associated with skin aging prevention. Accordingly, the cosmetic composition containing any one or more extracts selected from the group consisting of ginseng, golden, and ginseng as an active ingredient exhibits excellent skin aging prevention effect.

On the other hand, the present invention is preferably applied to Rehmannia glutinosa ), golden ( Scutellaria baicalensis ), and Taisho ( Isatis indigotica ) as an active ingredient. More preferably 10 to 50% by weight of lead, 10 to 50% by weight of gold, and 10 to 50% by weight of sea bream as an active ingredient. Mixed extracts exert a better anti-aging effect than single extracts.

On the other hand, in the present invention, the baechul may preferably be the root of the baechu.

Meanwhile, in the present invention, the extract is preferably extracted with ethanol as an extraction solvent. And more preferably 60 to 80% ethanol as an extraction solvent.

The term " comprising as an active ingredient " in the present invention means that the anti-aging effect of skin in the composition of the present invention is reversible ( Rehmannia glutinosa ), golden ( Scutellaria baicalensis ), Daechung ( Isatis indigotica ). < / RTI > However, the composition of the present invention Rehmannia (Rehmannia glutinosa), gold (Scutellaria baicalensis), may hall (including other raw materials into the different components or other cosmetic skin with anti-aging effect in addition to any one or more extracts selected from the group consisting of Isatis indigotica).

On the other hand, in the cosmetic composition for preventing skin aging according to the present invention, the skin aging can be preferably induced by thermal stimulation. According to the experiment of the present invention described below, Rehmannia glutinosa extract, golden ( Scutellaria baicalensis extract, Isatis indigotica extract and rhubarb ( Rehmannia glutinosa ), golden ( Scutellaria baicalensis ), Daechung ( Isatis indigotica ) was found to be effective against skin aging induced by thermal stimulation.

On the other hand, in the cosmetic composition for preventing skin aging according to the present invention, the skin aging may preferably be caused by wrinkles. In the following experiment, Rehmannia glutinosa extract, golden ( Scutellaria baicalensis) Extracts, hall (Isatis indigotica) extract and Rehmannia (Rehmannia glutinosa ), golden ( Scutellaria baicalensis ), Daechung ( Isatis mixing the extract of indigotica) is because it is confirmed to decrease the troponin elastin (tropoelastin) and the expression of MMP-12 that are associated with wrinkling and increasing the fibril Lin (fibrillin) -1 expression.

Meanwhile, the cosmetic composition of the present invention may be in the form of a suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, wax foundation, Spray, or spray.

When the formulation of the cosmetic composition of the present invention is a paste or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, Can be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, Propellants such as carbon, propane / butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is the above solution or emulsion, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan can be used.

When the formulation of the cosmetic composition of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth.

When the formulation of the cosmetic composition of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

On the other hand, the cosmetic composition of the present invention can be used alone or in combination with other ingredients. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use may be varied depending on the skin condition or taste of the user.

The present < RTI ID = 0.0 >Rehmannia glutinosa) Extract, golden (Scutellaria baicalensis) Extract, DaechungIsatis indigotica) Extracts and Rhubarb (Rehmannia glutinosa), Gold (Scutellaria baicalensis), DaechungIsatis indigotica) Suppresses the occurrence of various factors related to skin aging and promotes the activation of various factors associated with anti-aging. Therefore, the cosmetic composition of the present invention exhibits excellent skin aging-preventing effect.

Fig. 1 shows the results of the cytotoxicity evaluation of the extracts of Rhizobia, Golden and Daicheng roots.
FIG. 2 shows the results of evaluating the expression of the heat shock protein HSP47 in the mixed extracts, the Chinese cabbage extract, the golden extract and the Daechu root extract.
FIG. 3 shows the expression of tropoelastin, fibrillin-1 and elastin degrading enzyme (MMP-12) by the treatment of mixed extract, hwanghwang extract, golden extract, This is the result of evaluating the level.
FIG. 4 is a result of measuring changes in skin temperature when a formulation containing the mixed extract of the present invention (mixed extract of guinea pig, golden, and radish root) was actually used.
FIG. 5 is a graph showing the change in skin temperature visually using a thermal imaging camera when a formulation containing the mixed extract of the present invention (mixed extract of guinea pig, golden, and roe) is actually used.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

[ Example  1: Manufacture of mixed extract of Rhizopus, Golden, and Daechung Root]

The mixture was prepared by pulverizing a mixture obtained by mixing 1: 1: 1 of rhizome, golden, and radish roots at a ratio of 1: 1: 1. To 25 g of the prepared mixed powder, 500 ml of 70% ethanol as a solvent was added and the mixture was stirred with a stirrer (Heidoph, specifications: MR3001, MR3001K ) For 3 hours at 500 rpm. After the extraction, the extract solution was filtered using a filter paper, and the recovered filtrate was concentrated under reduced pressure at 45 ° C using a concentrator (Heidolph, LABOROTA 4000) to obtain a mixed extract.

In the present invention, the mixed extract of Rhododendron, Golden, and Daechung Root was referred to as 'mixed extract'.

[ Example  2: Manufacture of huangbu extract]

500 ml of 70% ethanol as a solvent was added to 25 g of zeolite powder and stirred and extracted at 500 rpm for 3 hours using a stirrer (manufacturer: Heidoph, standard: MR3001, MR3001K). After the extraction, the extract solution was filtered using a filter paper, and the filtrate was collected and concentrated under reduced pressure at 45 ° C using a concentrator (Heidolph, LABOROTA 4000) to obtain a rhizome extract.

[ Example  3: Preparation of gold extract]

500 g of 70% ethanol as a solvent was added to 25 g of the gold powder, followed by stirring and extraction at 500 rpm for 3 hours using a stirrer (manufacturer: Heidoph, standard: MR3001, MR3001K). After the extraction, the extract solution was filtered using a filter paper, and the recovered filtrate was concentrated under reduced pressure at 45 ° C using a concentrator (Heidolph, LABOROTA 4000) to obtain a golden extract.

[ Example  4: Manufacture of Daechung Root Extract]

500 g of 70% ethanol as a solvent was added to 25 g of Daechung root powder, followed by stirring and extraction at 500 rpm for 3 hours using a stirrer (manufacturer: Heidoph, standard: MR3001, MR3001K). After the extraction, the extract solution was filtered using a filter paper, and the filtrate was collected and concentrated under reduced pressure at 45 ° C using a concentrator (Heidolph, LABOROTA 4000) to obtain a root extract of Daechung.

[ Experimental Example  1: cytotoxicity evaluation]

In this test, the cytotoxicity of the extracts of Rhus javanicum, Golden extract, and Daechung root was evaluated by MTT assay.

Human fibroblasts (HDF) were cultured in each well of a 96-well plate at a concentration of 1 × 10 ⁵ cells / ml. Then, the horseradish extract, golden extract, 100 [mu] L of each solution was added to each well of the plate at a concentration of 25, 50, 100, and 200 [mu] g / mL, followed by incubation for 24 hours. Twenty-four hours later, 20 μl of MTT solution diluted in PBS was added to each well at a concentration of 5 mg / ml, and the mixture was incubated for 2 hours. Then, 100 μl of isopropanol was eluted with an ELISA reader (VICTOR3, Perkin Elmer) Absorbance at 570 nm.

After measuring the absorbance of the experimental group (sample treated group) and the control group (untreated group), the cytotoxicity evaluation was converted and the results are shown in Fig. Here, the cell viability (%) was obtained by the following formula (1).

As shown in FIG. 1, it was confirmed that the extracts of Rhododendron, Extract, and Daechung Root did not show cytotoxicity in the test concentration range (FIG. 1). Fig. 1 shows the results of the cytotoxicity evaluation of the extracts of Rhizobia, Golden and Daicheng roots.

[ Experimental Example  2: Heat shock protein  Evaluation of Expression]

Heat shock protein (HSP) is a kind of protective protein that protects skin cells from stress caused by in vivo / external harmful environment (heat, ultraviolet ray, etc.) and normal function of skin cells. Therefore, in this Experimental Example, changes in HSP expression by heat stimulation and changes in HSP expression by the treatment of the mixed extracts, ginseng extracts, golden extracts and Daechung root extracts obtained in Examples 1 to 4 were confirmed by RT-PCR Respectively.

Human-derived fibroblasts were inoculated on a 60 mm plate at 1 × 10 ⁶ cells / well and cultured for 24 hours. After the culture, the culture medium was discarded, and a serum-free medium was added thereto, followed by starvation for 24 hours. After 24 hours, the serum-free medium was added, and a plate sealed with parafilm was placed in a water bath adjusted to 42 to 44 ° C. After thermal stimulation for 15 minutes, The samples were diluted to a concentration of 100 μl / ml and treated with 3 ml each on each plate. After the treatment, the amount of HSP47 gene expressed from the cells cultured for 24 hours was examined.

The HDF cells cultured in the above were washed twice with DPBS buffer, and 1 ml of QIAzol was added to each plate. After reacting at room temperature for 5 minutes, the cells were recovered, and 200 쨉 l of chloroform was added thereto. Vortexing was performed. Thereafter, centrifugation was carried out at 4 占 폚 and 12,000 x g for 20 minutes to recover 400 占 퐇 of supernatant. Thereafter, 500 占 퐇 of isopropanol was added to each of the tubes, followed by vortexing and reaction at room temperature for 10 minutes.

Thereafter, centrifugation was carried out at 4 DEG C at 12,000 x g for 20 minutes to precipitate RNA, and the supernatant was discarded. Then, the RNA pellet was washed with 75% ethanol (centrifuged at 12,000 x g for 20 minutes at 4 ° C), air-dried, and DEPC H ₂ O was added to extract the RNA. After confirming total RNA by electrophoresis, RNA was quantified using a spectrophotometer, and first strand cDNA was synthesized. Subsequently, cDNA synthesis was performed using cDNA synthesis kit (Thermo, # K 1643) as follows. 4 μl of 5X reaction buffer and 2 μl of 'Maxima Enzyme Mix' were added to total RNA (0.5 μg), and then nuclease free water was added to a total volume of 20 μl, CDNA was synthesized by setting reaction conditions at 25 ° C for 10 minutes, at 50 ° C for 15 minutes, and at 85 ° C for 5 minutes in this order.

Then, cDNA amplification was performed as follows using 'EmeraldAmp GT PCR Master Mix, TAKARA, RR310A'. 1 μl cDNA synthesis and 12.5 μl master mix, 1.25 μl forward primer (10 pmol), 1.25 μl reverse primer (10 pmol) and 9 μl nuclease free water were mixed to prepare cDNA . The primer sequences used for the amplification of the target gene were as shown in Table 1 below, and the experimental results are shown in FIG.

Target (target) order HSP47 Forward primer 5'-CGT CTC GAC TCC TGG GAA TG-3 ' Reverse primer 5'-ACA TGG CGT TGA CTA GCA GG-3 '

As shown in FIG. 2, it was confirmed that the expression of HSP47 reduced by heat stimulation was increased in all of the mixed extract, hwanghwang extract, golden extract and Daechung root extract treated group. Among them, It was confirmed that the effect of increasing expression was the best (Fig. 2). FIG. 2 shows the results of evaluating the expression of the heat shock protein HSP47 in the mixed extracts, the Chinese cabbage extract, the golden extract and the Daechu root extract.

[ Experimental Example  3: Daylight fibrillogenesis  evaluation]

Sunlight Elastic Fibrosis, a typical phenomenon of photoaging, is a phenomenon in which an abnormal elastic fiber material accumulates in the dermis, and the elasticity of the skin is lowered to cause symptoms of skin sagging. In order to examine the effects of the mixed extracts, ginseng extracts, golden extracts and Daechung root extracts obtained in Examples 1 to 4 on daytime elastic fibrosis, the elastic fiber fibrosis, tropoelastin (tropoelastin), fibrillin-1 expression and elastin degrading enzyme (MMP-12) gene expression levels were evaluated by RT-PCR.

The RT-PCR method was the same as that of Experimental Example 2, and the primer sequences used for the amplification of the target gene were as shown in Table 2 below, and the results are shown in FIG.

Target (target) order Tropoelastin Forward primer 5'-AAA GCA GCA GCA AAG TTC GG-3 ' Reverse primer 5'-ACC TGG GAC AAC TGG AAT CC-3 ' Fibrillin-1 Forward primer 5'-GGT GAA TGT ACA AAC ACA GTC AGCA-3 ' Reverse primer 5'-ATA GGA ACA GAG CAC AGC TTG TTGA-3 ' MMP-12 Forward primer 5'-ACA CCT GAC ATG AAC CGT GAG-3 ' Reverse primer 5'-TGG CCA AGA CCT AAG GAA TG-3 '

As a result of the experiment, as shown in FIG. 3, the mixed extract, hwanghwang extract, golden extract and Daechung root extract decreased the expression of tropoelastin and MMP-12 which were increased by heat stimulation and decreased by heat stimulation The expression of fibrillin-1 was increased. Among them, it was confirmed that the mixed extract had the best effect of reducing the expression of tropoelastin and MMP-12 and increasing the expression of fibrillin-1 (FIG. 3). FIG. 3 shows the expression of tropoelastin, fibrillin-1 and elastin degrading enzyme (MMP-12) by the treatment of mixed extract, hwanghwang extract, golden extract, This is the result of evaluating the level.

[ Experimental Example  4: Human body Patch  Test]

The human patch test is widely used as a method for searching for primary irritants, but dermatologically refers to a diagnostic patch test for the diagnosis of allergic contact dermatitis. Therefore, in the present experimental example, it was determined whether the mixed extracts, hornblende extracts, golden extracts and Daechung root extracts obtained in Example 1 and Comparative Examples 1 to 3 were suitable for skin use.

The mixed extracts, ginseng extracts, golden extracts and Daechu root extracts obtained in Example 1 and Comparative Examples 1 to 3 were dissolved in 5% DMSO in a Finn chamber in 20 arms of healthy adult women. 2%, respectively, and they were fixed on the skin with a tape (scanpore tape). After 24 hours, it was removed from the skin. After 1 hour, the degree of erythema and edema was determined. The criteria were based on the criteria of the International Contact Dermatitis Research Group (ICDRG) listed in Table 3 below and the results are shown in Table 3 below.

sample Tested embroidery Judgment result Irritation degree ++ + ± - Mixed extract 20 - - - 20 0 Ginger extract 20 - - - 20 0 Golden extract 20 - - - 20 0 Daechung root extract 20 - - - 20 0 Control group 20 - - - 20 0 (Criteria)
-: No erythema or unusual phenomenon
±: slightly redder than around
+: Significantly reddened than surrounding
++: More reddening and swelling than the surrounding area
(Stimulus calculation formula)
The number of stimuli = [{(占) number 占 1} + {占 number 占 2} + {(++) number 占 3}

As a result of the experiment, as shown in Table 3, it was confirmed that no symptoms were observed in 20 female subjects. Therefore, it was confirmed that the mixed extract, hwanghwang extract, golden extract and Daechung root extract of the present invention are suitable for use as cosmetics.

[ Experimental Example  5: Prototype cream Train terminal  Efficacy evaluation]

The present experiment was conducted to evaluate the efficacy of a cream (Formulation Example 1) to which a mixed extract of the present invention (mixed extract of ginseng, gold, and roots of Daechung) was added (Protective Example 1) as a prototype, and then to evaluate its effect on the temporary heat shielding effect. The skin temperature before and after use of the present invention cream with the prescription of Table 4 below was measured for women aged 20 to 59 years.

ingredient
W.T (%) Water Purified water 73.7545 Glycerin glycerin 5 IsoamylLaurate Isoamyl laurate 3 Glycereth-26 Glycereth-26 3 Titanium Dioxide Titanium dioxide 3 Biosaccharide Gum-1 Bai Osaka Ride Gum -1 2 Arbutin Arbutin 2 Alcohol ethanol 2 ButyleneGlycol Butylene glycol 1.144 Sodium Acrylate / Sodium Acryloyldimethyl Taurate Copolymer Sodium acrylate / sodium acryloyldimethyltaurate copolymer 0.5 PropyleneGlycol Propylene glycol 0.01 MorusAlbaBark Extract Eucalyptus extract 0.04 Scutellaria Baicalensis Root Extract Golden extract 0.3 Rehmannia Glutinosa Root Extract Ginger extract 0.3 Isatis Tinctoria Root Extract Daechung root extract 0.3 Prunus Serrulata Flower Extract Cherry extract 0.007 Pearl Extract Pearl extract 0.007 Lonicera Caprifolium (Honeysuckle) Flower Extract Rhododendron flower extract 0.007 Paeonia Lactiflora Root Extract Peony extract 0.006 Jasminum Officinale (Jasmine) Flower Extract Jasmine flower extract 0.006 Chamomilla Recutita (Matricaria) Flower / Leaf Extract Chamomile flower / leaf extract 0.006 DunaliellaSalina Extract Dulnellella salina extract One StearicAcid Stearic acid 0.4 Potassium Cetyl Phosphate Potassium cetyl phosphate 0.25 Aluminum Hydroxide Aluminum hydroxide 0.25 Polysorbate 80 Polysorbate 80 0.2 Isohexadecane Isohexadecane 0.425 Caprylic / Capric Triglyceride Caprylic / capric triglyceride 0.005 Lecithin lecithin 0.002 Hexylene Glycol Hexylene glycol 0.21 Ethylhexylglycerin Ethylhexyl Glycerin 0.07 Decylene Glycol Decylene glycol 0.07 Totarol Totally 0.14 Allantoin Allantoin 0.1 Dipotassium Glycyrrhizate Dipotassium glycyrrhizate 0.05 Bis-Stearyl PEG / PPG-8/6 SMDI / PEG-400 Copolymer Bis-stearyl phage / PPE-8/6 SMD / PPE-400 copolymer 0.2 Ammonium Acryloyldimethyltaurate / VP Copolymer Ammonium acryloyldimethyltaurate / Vpicopolymer 0.05 Adenosine Adenosine 0.04 Caprylyl Glycol Caprylic glycol 0.025 Phenoxyethanol Phenoxyethanol 0.0255 Fragrance Spices 0.1 system 100

As a result of measuring the skin temperature, as shown in FIG. 4, when the formulation of the present invention was used, even if the skin temperature was a little, the skin temperature was rather low. In the case of using the formulation 1, Respectively. FIG. 4 is a result of measuring changes in skin temperature when a formulation containing the mixed extract of the present invention (mixed extract of guinea pig, golden, and radish root) was actually used.

On the other hand, FIG. 5 shows the result of measurement of change in skin temperature at the time of heating after application of the face of Formulation Example 1 using a thermal imaging camera. As shown in Fig. 5, when the formulation of the present invention was used, it was visually confirmed that the skin temperature did not rise. FIG. 5 is a graph showing the change in skin temperature visually using a thermal imaging camera when a formulation containing the mixed extract of the present invention (mixed extract of guinea pig, golden, and roe) is actually used.

Claims (7)

Rehmannia glutinosa ), golden ( Scutellaria baicalensis) and the hall (Isatis indigotica) aging to any one or more extracts selected from the group consisting of characterized in that it contains as an active ingredient for preventing cosmetic composition.
The method according to claim 1,
Rehmannia glutinosa ), golden ( Scutellaria baicalensis) and skin anti-aging composition for the mixed extract of the hall (Isatis indigotica), characterized in that it contains as an active ingredient.
3. The method of claim 2,
The above-
10. The composition for preventing skin aging as set forth in any one of claims 1 to 9, wherein the composition comprises 10 to 50% by weight of zinc oxide, 10 to 50% by weight of gold and 10 to 50%
The method according to claim 1,
The above-
A cosmetic composition for preventing skin aging characterized by being root of Daechung.
The method according to claim 1,
The above-
Wherein the extract is extracted with ethanol as an extraction solvent.
The method according to claim 1,
Skin aging,
A cosmetic composition for preventing skin aging characterized by being induced by thermal stimulation.
The method according to claim 1,
Skin aging,
A cosmetic composition for preventing skin aging characterized by wrinkles.

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