KR20160123628A - Cosmetic composition comprising disporum sessile extract - Google Patents

Abstract

The present invention relates to a skin cosmetic composition comprising a Disporum uniflorum Baker extract obtained by using extraction methods using water, organic solvents, or ultrasonic waves, through supercritical, fermentation, and processing. The Disporum uniflorum Baker extract has an excellent skin aging prevention effect due to an excellent skin antioxidant effect and an excellent cell proliferation effect.

Description

COSMETIC COMPOSITION COMPRISING DISPORUM SESSILE EXTRACT [0002]

More particularly, the present invention relates to a cosmetic composition comprising a radish herb extract obtained by using water, an organic solvent, or an extraction method such as supersonic or supercritical fluid. will be.

Free radicals, which cause skin aging in general, are highly chemically reactive chemicals. They are caused by various factors in the human body such as ultraviolet rays, radiation, smoke, smoke, stress and metabolism of the mitochondria and leukocytes in the body And the lipid peroxidation, which is a component of the cell membrane, is produced through a multistage chain reaction.

Most of the free radicals associated with skin aging are Reactive Oxygen Species (ROS), and the lipid peroxidation caused by them causes a variety of reactive oxygen species of radicals, peroxides, aldehydes and epoxides And damages DNA, RNA, proteins, cell membranes and cell structures.

So far, research on antioxidants has been one of the most studied areas for inhibiting skin aging. It is known that skin is oxidatively stressed by radicals such as superoxide radicals and hydroxyl radicals and non-radicals such as hydrogen peroxide (H ₂ O ₂ ), thereby damaging cells and promoting skin aging. Therefore, not only the direct antioxidant effect on various oxidizing substances but also the suppressive effect of TNF-a and IL-8 secretion from keratinocytes suppresses oxidative stress of cells, and after that, TNF-a and IL-8 secreted from keratinocytes There is a continuing research on antioxidants that inhibit secondary reactions to peripheral skin cells such as Langerhans cells, fibroblasts, and melanocytes to fundamentally prevent skin aging.

These antioxidants that prevent skin damage by active oxygen include superoxide dismutase (SOD), enzymes such as catalase and tocopherol (vitamin E), beta- carotene, Lipid soluble and water soluble antioxidants or radical scavengers such as ascorbic acid (vitamin C) and glutathione.

As described above, since free radical oxygen or reactive oxygen species are highly related to skin aging, conventionally, superoxide dismutase, vitamin E derivatives, vitamin C and derivatives thereof, and flavonoids have been added to cosmetics There have been a lot of efforts to achieve the skin aging prevention effect by mixing the above ingredients. However, when the above ingredients are mixed in cosmetics, safety, stability, and the like are poor, and it is difficult to expect a substantial effect.

Therefore, in order to solve the problems of the prior art described above, there is a continuing need to develop new natural products and products having excellent antioxidative effect and excellent cell proliferation effect.

According to Korean Patent Laid-Open No. 10-2012-0064218, it is known that the extract of Panax ginseng Radix extract obtained by extracting the ground part of the radish herb with methanol as a solvent has an anti-inflammatory effect. When treated with mouse macrophages, (INOS), which is a gene that regulates the production of nitric oxide, is also effective in reducing the expression of the nitric oxide synthase (NOS).

Accordingly, it is an object of the present invention to provide a cosmetic composition having excellent antioxidative effect and excellent cell proliferation effect. It is another object of the present invention to provide a cosmetic composition having an anti-aging effect through an effect of removing active oxygen in the skin and protecting the skin from cell damaging substances and enhancing collagen biosynthesis by promoting proliferation of skin cells have.

The present invention relates to a cosmetic composition for preventing skin aging which contains an extract of a radish herb extract.

Preferably, the cosmetic composition has a skin antioxidant effect.

Preferably, the cosmetic composition has cell migration effect.

Preferably, the cosmetic composition has an effect of improving skin elasticity.

Preferably, the herb extract of the present invention contains 0.001 to 30.0% (w / w) based on the total weight of the cosmetic.

Preferably, the shrub herb extract is a stem or root extract of a shrub herb.

Preferably, the edible oil extract of the present invention is obtained by dissolving the edible oil of the present invention in a solvent selected from the group consisting of water, anhydrous or lower alcohols having 1-4 carbons, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl acetate, chloroform, diethyl ether, , Extraction with an extraction solvent selected from the group consisting of acetone, hexane, and mixtures thereof, ultrasonic extraction, supercritical extraction, fermentation, and a foaming method.

Preferably, the cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, Lt; / RTI >

The present invention relates to a cosmetic composition for preventing skin aging comprising an extract of a radish herb extract. The cosmetic composition of the present invention, which is an effective ingredient, has a superior antioxidative and cell migration effect on the skin and excellent skin elasticity, and thus has excellent efficacy as a cosmetic for preventing skin aging.

1 shows the cell migration effect of the herbal extract of the present invention.

The present invention provides a skin cosmetic composition comprising a radish herb extract.

Disporum sessile D. Don used as an active ingredient in the cosmetic composition of the present invention is a perennial herbaceous plant belonging to the lily family and grows in the shade of the forest of the forest. It is distributed in Korea, Japan, and Sakhalin. It grows in the mountains of Ulleungdo and Jeju Island. It is a beautiful flower, easy to reproduce and easy to cultivate, so it is suitable for ornamental use. Since ancient times, coughs, diapers, and pulmonary tuberculosis have been used as medicines.

In the present invention, the extract of the radish herb extract means an extract obtained by extracting from various organs (for example, leaves, flowers, fruits, stems, branches, roots) of the radish herb, preferably using the stem or root of the radish herb. will be.

The herb extract of the present invention can be prepared by conventional methods known in the art, that is, under the conditions of ordinary temperature and pressure, using a conventional solvent (for example, water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms , Acetone, ethyl acetate, butyl acetate or 1,3-butylene glycol).

According to a preferred embodiment, the method for producing the edible herb extract of the present invention is as follows. First, the shredded herbs are cleanly washed with purified water, dried and crushed into small pieces, and then added with water of 1 to 10 times the dry weight, anhydrous or lower alcohol of 1 to 4 carbon atoms, acetone, ethyl acetate, 1,3-butylene glycol and the like are added. Then, it is extracted by heating at 40 to 100 ° C for 3 to 20 hours or at 4 to 40 ° C for 1 to 15 days in a state where a cooling condenser is installed to prevent evaporation of the active ingredient, and is completely dried by a rotary evaporator . In this case, in the case of 1,3-butylene glycol, it is difficult to dry using a rotary evaporator, so it is extracted directly under the above conditions and adjusted to a 1% (w / v) loss on drying.

On the other hand, the edible herb extract of the present invention includes not only the extract obtained by the above-mentioned extraction method, but also the extract obtained by supercritical extraction or ultrasonic extraction by decompression by carbon dioxide and high temperature.

Decompression by carbon dioxide and extraction by supercritical extraction by high temperature means supercritical fluid extraction. Generally, supercritical fluids have the properties of liquid and gas when gas reaches the critical point at high temperature and high pressure, and chemically have polarity similar to that of nonpolar solvent. Due to this characteristic, supercritical fluid (J. Chromatogr. A. 1998; 479: 200-205).

Carbon dioxide is a supercritical fluid with both liquid and gaseous nature, with the operation of supercritical fluid equipment reaching its critical pressure and temperature, resulting in increased solubility in fat-soluble solutes. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the lipophilic substance contained in the sample is extracted into supercritical carbon dioxide.

When the supernatant carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction vessel after extracting the lipid-soluble substance, components that were not extracted by pure supercritical carbon dioxide can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract an active ingredient by using a mixed fluid in which a cosolvent is further mixed with supercritical carbon dioxide or carbon dioxide. These co-solvents may be used alone or in admixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether.

Most of the extracted samples contain carbon dioxide. Since the carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the cosolvent can be removed by a reduced pressure evaporator.

As the extraction solvent which can be used for the ultrasonic extraction method, one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether can be used.

The extracted sample is recovered by vacuum filtration, and the filtrate is recovered, and the extract is removed by a vacuum evaporator and freeze-dried to obtain an extract.

In addition, the herbal extract of the present invention also includes extracts which have undergone conventional purification and / or fermentation processes. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, The fraction is also considered to be included in the extract of the present invention. The fermentation product obtained by fermentation for 1 to 7 days while maintaining the pH of 5 to 7 under a temperature condition of 20 to 40 DEG C as a bacterium selected from the group consisting of yeast, lactic acid bacteria, Bacillus subtilis bacteria and mixtures thereof, It is interpreted to be included in the extract.

In addition, the herb extract of the present invention also includes the extract obtained by the pulverization method. Fojeran is a pharmaceutical technology that changes the original properties of medicines by processing the medicines based on the oriental medicine theory. Mainly refers to the process of steaming, boiling, roasting, roasting, burning, or mixing processes. In the present invention, the edible product was steamed and dried before use.

The herb extract of the present invention can be prepared in a powder state by an additional process such as vacuum distillation and freeze drying or spray drying.

According to a more preferred embodiment of the present invention, the herb extract of the present invention is excellent in antioxidative and cell migration (cell migration) and skin aging effects.

According to a preferred embodiment of the present invention, the content of the herbal extract of the present invention is 0.001 to 30.0% by weight, preferably 0.1 to 20.0% by weight based on the total weight of the cosmetic composition. At this time, when the total weight of the extract is less than 0.001% by weight, the effect thereof is difficult to be exhibited. When the total weight exceeds 30.0% by weight, there is a high possibility of irritation to the skin and a great influence on the stabilization of the formulation.

The components contained in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the extract of the carnauba wax extract as an active ingredient and include conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, And a carrier.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto.

Example  One. Edible herb  Preparation of extract

100g of washed and dried shrubs (stem and root) were powdered and made fine using a mesh. Thereafter, 1,000 ml of 70% ethanol adjusted to a ratio of water and ethanol of 7: 3 was added and the mixture was leached at room temperature for 4 days. The solution was filtered and the filtrate was concentrated in vacuo to obtain a dry extract. The extraction yield was 24.2 g / 100 g. The dried extract was dissolved in 30% 1,3-butylene glycol solution and purified water to a concentration of 10% (w / v), filtered, and applied to the experiment of the present invention.

Experimental Example  One. Edible herb  Extract NBT Antioxidant effect measurement

In Experimental Example 1, antioxidative activity was measured by the NBT method using BHT (Butylated Hydroxytoluene), a substance known to have antioxidative effect, as a comparative group in order to confirm the antioxidative effect of the extract obtained from Example 1.

In order to measure the antioxidant effect, active oxygen produced by xanthine and xanthine oxidase was measured by NBT method, and the effect of the test substance (Bamboo shoot extract, BHT) to remove active oxygen, that is, do. Xanthine and xanthine oxidase produce active oxygen. The active oxygen is reacted with Nitro Blue Tetrazolium (NBT), and the blue color produced by the reaction is measured at a wavelength of 560 nm to measure the active oxygen scavenging ratio (%).

As a measuring method

1. 0.05 M Na ₂ CO ₃ Solution -------------------------------- 2.4ml

2. 3 mM xanthine solution - 0.1 ml

3. 3 mM EDTA solution ------------------------------------ 0.1 ml

4. BSA solution ----------------------------------------- 0.1ml

5. 0.72 mM NBT solution - 0.1 ml

6. Xanthine oxidase solution - 0.1 ml

7. 6 mM CuCl ₂ solution - 0.1 ml

① Add 1, 2, 3, 4 and 5 solutions to vials, add 0.1 ml of sample solution and leave at 25 ℃ for 10 minutes.

② Add 6 solution, stir rapidly, and start culturing at 25 ℃ for 20 minutes.

③ Then, add 7 solution to stop the reaction and measure the absorbance St at 560 nm.

(4) The blank of the sample solution is measured by the same procedure as that for measuring the absorbance St except that distilled water is used instead of the above-mentioned 6 solution.

(5) The absorbance Bt is measured by operating the blank test in the same manner as in the measurement of the absorbance St except that distilled water is used instead of the sample solution.

(6) Blank of the blank test is carried out in the same manner as in the measurement of the absorbance St except that distilled water is used instead of the sample solution and the 6 solution, and the absorbance Bo is measured.

The active oxygen scavenging rate (%) was calculated according to Equation (1), and the results are shown in Table 1.

St: Absorbance at 560 nm after enzyme reaction of sample solution

Bt: Absorbance at 560 nm after enzyme reaction of blank test solution

So: the absorbance at 560 nm before the reaction in the absence of enzyme in the sample solution

Bo: absorbance at 560 nm before the reaction in the absence of enzyme in the blank test solution

Name of sample Treatment concentration (%) Antioxidative effect (%) Example 1 0.01 87.9 BHT 0.01 90.1

As shown in Table 1, the test results showed excellent antioxidative effects at the concentration of 0.01%. It was confirmed that the extract of the present invention has almost similar antioxidative effect as that of BHT at a concentration of 0.01%.

Experimental Example 2. Measurement of Antioxidative Effect of Panaxgillus oryzae Radix Extract Extract Using DPPH Method

In order to measure the antioxidative effect of the extract of the radish herb extract obtained in Example 1, Experimental Example 2 was carried out in the same manner as in Example 1 except that vitamin C, which is known to have an antioxidative effect, was used as a comparative group and the antioxidative effect (free radical scavenging ratio) Were measured.

The DPPH method measures the antioxidative effect by reducing power using a free radical called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical. The degree of free radical scavenging (%) at a wavelength of 560 nm is measured by comparing the degree of decrease of the absorbance by DPPH reduction by the test substance (the radiolabeled extract, vitamin C) with the absorbance of the blank test solution.

As a reagent used, 61.88 mg of a 0.1 mM solution of 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 618.76) was dissolved in methanol to make 100 ml.

As a measuring method,

(1) Add 0.15 ml of the sample solution to 0.15 ml of 0.1 mM DPPH solution in a 96-well plate, stir rapidly, and start culturing at 25 ° C for 10 minutes.

② Measure the absorbance St at 560 nm thereafter.

(3) The blank of the sample solution is measured by the same procedure as that for measuring the absorbance St except that methanol is used instead of the 0.1 mM DPPH solution.

(4) The absorbance Bt is measured by operating in the same manner as in the measurement of the absorbance St except that distilled water is used instead of the sample solution.

(5) Blank of the blank test is carried out in the same manner as in the measurement of the absorbance St except that methanol is used instead of the 0.1 mM DPPH solution and distilled water is used instead of the sample solution, and the absorbance Bo is measured.

The results of the free radical scavenging ratio (%) were calculated according to Equation (2), and the results are shown in Table 2 below.

St: absorbance at 560 nm after free radical scavenging of the sample solution

Bt: absorbance at 560 nm after free radical scavenging of the blank test solution

So: the absorbance at 560 nm before the reaction in the absence of free radicals in the sample solution

Bo: absorbance at 560 nm before the reaction in the absence of the free radical of the blank test solution

Name of sample Treatment concentration (%) Antioxidative effect (%) Example 1 0.05 89.1 Vitamin C 0.05 93.4

As shown in Table 2, it was confirmed that the extract of Panax ginseng extract had antioxidative effect similar to the antioxidative effect of vitamin C, which is known to have an antioxidative effect at a concentration of 0.05%.

Experimental Example 3: Cell migration effect

Analysis of the promoting effect of migration of fibroblasts is one of the common methods of measuring the wound healing effect of cells. Therefore, the fibroblast HDFn cells were confluently cultured in a 24-well plate, and the cells were scraped (in vitro wounding) using a microtip, treated with 1 ppm and 1000 ppm of the Panax grape extract of Preparation Example 1, and reacted for 24 hours. After 24 hours, the cells migrated under the microscope were photographed and the density of cells transferred to the wounding location was determined.

FIG. 1 shows the cell migration effect of the spinach extract obtained in Example 1 of the present invention.

Migration evaluation in the HDFn cell confirms cell migration to the empty space after 24 hours. As can be seen from FIG. 1, cells were observed in the middle space after 24 hours at each concentration in Example 1 as compared with the control group, showing excellent cell migration effect.

Experimental Example 4: Evaluation of skin elasticity improving effect

In order to confirm the effect of improving the skin elasticity of the extract of the radish herb extract obtained in Example 1 of the present invention, a cosmetic (Formulation Example 1) comprising a composition containing a radish herb extract was prepared, The following experiment was conducted. As shown in Table 3, the cosmetic compositions of Comparative Formulation Example 1, which is a control group not including the extract of the radish herb extract, were prepared, and the skin elasticity improving effect was compared.

The skin elasticity improvement effect of the cream containing Formulation Herb Extract (Formulation Example 1) and Comparative Formulation Example 1 was evaluated as follows.

In 15 subjects (20 to 35 year old female), the cream of Formulation Example 1 was applied to the right side of the face and the cream of Comparative Formulation Example 1 was applied to the left side of the face for 2 consecutive days for 4 consecutive weeks.

The skin elasticity improvement effect after the completion of the experiment was measured using a skin elasticity meter (SEM 575, C + K Electronic Co., Germany) before and 4 weeks after use of the product. The results are shown in Table 4 as the ΔR8 value of the skin elasticity meter (Cutometer SEM 575). The R8 value indicates the viscoelasticity of the skin.

As shown in the following Table 4, it can be seen that the skin elasticity improving effect of the test person who applied the cream of Formulation Example 1 containing the extract of the radish herb extract was excellent.

Unit: wt% Raw material Formulation Example 1 Comparative Formulation Example 1 One Stearyl alcohol 6.0 6.0 Stearic acid 2.0 2.0 Stearic acid cholesterol 2.0 2.0 Squalane 4.0 4.0 Caprylic / capric triglyceride 6.0 6.0 FAGE -100 stearate 2.0 2.0 Glyceryl stearate 2.0 2.0 2 Butylene glycol 6.0 6.0 3 Example 1 3.0 - 4 Purified water to 100 to 100

Experimental product
Skin elasticity effect (ㅿ R8, n = 15, p <0.05) Before use after use Formulation Example 1 0.12 0.38 Comparative Formulation Example 1 0.11 0.17

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (8)

A cosmetic composition for preventing aging of the skin containing an extract of a radish herb extract. The cosmetic composition according to claim 1, wherein the cosmetic composition has skin antioxidant effect. The cosmetic composition according to claim 1, wherein the cosmetic composition has cell migration effect. The cosmetic composition according to claim 1, wherein the cosmetic composition has an effect of improving skin elasticity. [Claim 2] The cosmetic composition according to claim 1, wherein the cosmetic composition comprises 0.001 to 30.0% (w / w) based on the total weight of the cosmetic. 2. The cosmetic composition according to claim 1, wherein the limulus herb extract is a stem or a root extract of a radish herb. [Claim 2] The method according to claim 1, wherein the edible oil extract is selected from the group consisting of water, anhydrous or lower alcohols having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl acetate, chloroform, Wherein the cosmetic composition is obtained from an extraction method selected from the group consisting of a solvent extraction method, an ultrasonic extraction method, a supercritical extraction method, a fermentation method and a foaming method using an extraction solvent selected from the group consisting of dichloromethane, hexane and mixtures thereof. The cosmetic composition according to claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Lt; RTI ID = 0.0 &gt; 1, &lt; / RTI &gt;

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