KR20160093585A - Vessels for plant tissue culture and domestic bioreactor containing the same - Google Patents

Abstract

The present invention relates to a plant tissue culturing body aseptic culturing container (100), an aseptic culturing system, and a home aseptic culturing device including the same. According to the present invention, the plant tissue culturing body aseptic culturing container comprises: a container body (10) containing a culturing liquid and a plant tissue culturing body; an air feeding hole (20) formed in one lower side surface of the container body (10), and feeding sterilized air; a backflow preventing valve (30) for passing air fed from the air feeding holes (20), and preventing a leakage of the culturing liquid contained in the container body (10); a porous sparger (40) protruding in a semispherical shape on the lower surface thereof in the container body (10), spraying air fed through the air feeding hole (20) into the container body to supply air to the plant tissue culturing body, and agitating the culturing liquid and the plant tissue culturing body; an upper cover (50) for covering the container body (10); and an exhaust hole (60) protruding on the upper surface of the upper cover (50), and discharging a gas generated from the inside of the container body (10) to the outside.

Description

Technical Field [0001] The present invention relates to a sterile culture vessel for plant tissue culture and a sterile culture apparatus for domestic use containing the same,

The present invention relates to a sterile culture container for a plant tissue culture, an aseptic culture system, and a sterile culture apparatus for home use comprising the same. More particularly, the present invention relates to a technique for culturing various plant tissue cultures in a sterile state at home .

Since ancient times, plants have been a source of many natural materials useful to humans, and the materials produced from them have been used for medicines, foods, and cosmetics. However, there are a number of problems such as when the natural substances useful are directly obtained from the plants, when the plants are rare species, when the growth is slow, and when the natural composition changes or the production amount decreases depending on the natural environment. These problems can be solved by using "Plant Tissue Culture Technology", which is to cultivate plant-derived cells and tissues in nutrient media under aseptic conditions. Plants have "Totipotency", the ability to regenerate complete plants from a part of the tissue, which allows tissue culture to take advantage of the potential capabilities of these plants.

In order to produce useful substances using plant tissue culture, mass production techniques are required, and a bioreactor is used here. A bioreactor is a device that allows an organism to grow under an artificially controlled environment for the purpose of producing a specific substance or cell, or performing a specific reaction. In order to produce a useful substance by using a tissue culture of a plant, a highly productive cell line should first be selected and a process of optimizing conditions of external factors such as medium composition, pH, temperature, and light should be performed. Once these conditions are met, a bioreactor suitable for the process and suitable for the growth of the tissue culture and the production of useful materials should be selected. The nature of the plant tissue culture sensitive to shear and whether the oxygen supply can be facilitated is also reviewed Should be.

Therefore, in order to select a suitable bioreactor, not only the biological, biochemical, physical / chemical characteristics of the organism and the product, but also the design of the whole process and commercialization of the product according to these characteristics should be considered. It can be said that the prudent selection of an appropriate reactor is important in establishing optimal production conditions that determine the economics and competitiveness of the final product.

The medium for plant tissue culture contains bulk elements, trace elements, vitamins and sugars necessary for growth, and can be produced at a relatively low cost. Since the protein is hardly contained, separation / purification of proteins from the plant is easy . In addition, it is relatively easy to cultivate large amount of cells compared to animal cell cultures, and has the advantage of being able to produce safe raw materials because there is no danger due to viruses or toxins.

Bioreactors are used in the global market in 2000 as chemicals ($ 13.5 billion), pharmaceuticals ($ 12 billion), energy ($ 18 billion), food ($ 18 billion) and agricultural products . In Japan, for the first time, commercialization of Shikonin, which can be used as a pigment for medicines, food and cosmetics, from Lithospermum erythrorhizon has been carried out through industrial application studies such as mass production of Taxol, It was recognized as a universal technology.

In Korea, bioreactor is an essential equipment for biotechnology that produces high value added raw materials by using living organisms such as microorganisms, plant cells and animal cells. In the past, it was known as fermenter and was used for fermentation of enzymes, amino acids and xylitol. On the other hand, studies have been actively conducted to cultivate wild ginseng cultured muscle using a bioreactor in Korea. Ginseng ( Panax ginseng CA Meyer) is a perennial herbaceous plant belonging to Araliaceae. It is a rare example of plants that are not readily available because of their extremely low number of spontaneous survival compared with long-known medicinal properties, The ginseng cultivar produced by tissue culture is not affected by environmental factors such as weather, pests and seasons, and is cultivated in aseptic condition under difficult cultivation conditions. Therefore, it is not contaminated with heavy metals and pesticide It can be produced with environmentally friendly agricultural products that are cultivated and cultivated.

Korean Unexamined Patent Application Publication No. 2005-008240 entitled "Domestic wild ginseng culture apparatus for producing crude ginseng and adventitious roots of wild ginseng ", " a sterilized filter installed in a lid portion of a culture vessel is supplied with sterilized air into a culture vessel, , And a domestic ginseng incubator having a lid of a culture container designed to be used at the lid for discharging air. Korean Patent Laid-Open Publication No. 1999-012384 " Bioreactor "as used herein refers to a bioreactor for culturing an animal, a microorganism, and a plant cell, wherein a lower end of the body forms a gentle inward curved surface, and an air supply pipe is attached to the lower part. .

In order to cultivate plant tissues, a variety of experimental tools are needed in addition to sterilized, high-pressure sterilizers, scales, distilled water, various nutrients, pH measuring instruments, and culture chambers. However, since these devices and apparatuses are expensive or dangerous and are not easy to use by the general public, it is difficult to cultivate aseptically using plant tissue culture at home.

In addition, in the current laboratory, devices necessary for cultivation are used only once for cultivation through a single sterilization operation, and there is a problem that the sterilization operation is performed every time the cultivation is performed. In addition, in the above-described conventional technology, there is a structure in which the culture is circulated by the air entering from the central entrance of the incubator. In this case, since circulation of air can not be performed in all the spaces inside the incubator, There is a problem that the growth of the cultured body is slow or the required cultured body can not be obtained.

The present inventors have recognized this problem and attempted to develop a plant tissue culture aseptic culture apparatus which can easily produce and directly use a plant tissue culture product without expensive equipment or complicated sterilization operation using the same at home. .

Accordingly, it is an object of the present invention to provide a culture container capable of maintaining and culturing a plant tissue culture in an incubator in a sterile condition at home.

It is another object of the present invention to provide an aseptic culture system using the culture container.

It is another object of the present invention to provide an apparatus for aseptic culture of a plant tissue culture which can easily cultivate a plant tissue culture product without complicated sterilization operation including the culture container.

In order to achieve the above object, according to the present invention, there is provided a method of manufacturing a sterilizing container, comprising: a container body for containing a culture liquid and a plant tissue culture; an air inlet formed at one side of the lower portion of the container body, And a backflow prevention valve which prevents the leakage of the culture liquid contained in the container body, and a backflow prevention valve which protrudes in the form of a hemisphere on the inner bottom surface of the container body, A porous sparger for stirring the culture liquid and the plant tissue culture product, an upper lid covering the container body, and an upper lid protruding from the upper surface of the upper lid, And an exhaust port for discharging the gas generated in the aseptic culture container to the outside .

Preferably, the body of the aseptic culture container is made of a transparent or semitransparent material. The inner surface of the container body is preferably subjected to an antibacterial treatment.

Preferably, the porous spar is formed to protrude into the container at a position eccentric from the center of the bottom of the container body. Through this, it is prevented that the tissue culture body adheres to the wall of the container or accumulates in some space, and uniform nutrition and oxygen supply are achieved. A porous spar cover may be formed on the porous sparger to prevent a hole of the porous sparger from being clogged with foreign substances or to prevent foreign matter from being mixed into the culture liquid through the porous sparger. At this time, it is preferable that the cover of the porous sparger forms holes alternately with the holes formed on the surface of the porous sparger.

In addition, in the porous sparger, it is preferable that a plurality of holes formed on the surface of the porous sparger to be a path of external air are formed to have different sizes.

It is preferable that the air outlet is provided with an air filter for preventing contamination from outside. It is also preferable that the exhaust port is formed to face the side of the container body in order to prevent contamination due to contaminants falling vertically.

According to another aspect of the present invention, there is provided an air pump comprising: an air pump; a sterilizing filter for sterilizing air supplied from the air pump; a regulating valve for regulating the amount and pressure of air supplied from the air pump; An air supply device connected to the air inlet of the aseptic culture container and connected to the sterilized culture container for injecting the sterilized air into the sterile culture container; And

A container body for containing a culture liquid and a plant tissue culture body; an air inlet formed at one side of the lower portion of the container body for injecting sterilized air; and a leakage of the culture liquid contained in the container body, The air is supplied to the plant tissue culture body by injecting the air injected through the air inlets into the container body. The air is supplied to the plant tissue culture body, A porous sparger for agitating the culture liquid and the plant tissue culture body, an upper lid covering the container body, and an exhaust port protruding from the upper surface of the upper lid and exhausting the gas generated inside the container body to the outside A plant tissue culture incubator, and a sterile culture container.

According to another aspect of the present invention, there is provided an air conditioner comprising: a lower body of an incubator in which the air supply device is installed; And

A plant tissue culture aseptic cultivation apparatus comprising an incubator main body in which a plant tissue culture aseptic culture container is installed,

A culture container support base is formed on the bottom surface of the incubator body so as to be rotatably supported by the aseptic culture container of the plant tissue culture support, and a front surface portion of the incubator main body is provided with an internal confirmation window, And an inner illumination device is formed on an upper surface of the incubator main body.

At this time, the shape of the incubator main body is not limited, and it may be formed in a cylindrical shape or a polygonal shape.

A temperature sensor and a fan for sensing the temperature of the inside of the incubator body may be additionally provided. A mood lamp for indoor illumination may be further provided on the upper outer surface of the incubator.

Since the aseptic culture vessel of the present invention has an air inlet, a backflow prevention valve and a porous sparge system, it is easy to maintain sterilization and sterilization, and the aseptic culture vessel in which sterility is maintained can be easily replaced . Such a system for providing sterile culture containers enables easy and convenient aseptic cultivation even in homes and laboratories because it is expensive, difficult to handle by ordinary people, dangerous devices, and without special cultivation technology.

1 is a cross-sectional view of an aseptic culture container for a plant tissue culture according to an embodiment of the present invention.
2 is an enlarged bottom cross-sectional view of an aseptic culture container of a plant tissue culture according to an embodiment of the present invention.
FIG. 3 shows a state in which an air supply device according to an embodiment of the present invention is coupled to a culture container.
FIG. 4 shows a culture system in which an air supply device and an aseptic culture container for plant tissue culture are connected according to an embodiment of the present invention.
5 and 6 illustrate an aseptic culture apparatus according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail with reference to the drawings.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a longitudinal sectional view of a plant tissue culture aseptic culture container according to one embodiment of the present invention. FIG.

According to one embodiment of the present invention, as shown in FIG. 1, a container body 10 for containing a culture medium and a plant tissue culture body is formed on one side of the lower part of the container body 10, and sterilized air is injected A backflow prevention valve 30 for passing the air injected from the air inlet 20 but preventing the leakage of the culture liquid contained in the inside of the container body 10, The air injected through the air inlet 20 is injected into the container body to supply air to the plant tissue culture body, and the porous body for stirring the culture liquid and the plant tissue culture body A sparger 40, an upper lid 50 covering the container body 10 and an upper lid 50 protruding from the upper surface of the upper lid 50, The exhaust port 60 for discharging the exhaust gas The plant tissue cultures sterile culture container 100 is provided.

At this time, it is preferable that the body of the aseptic culture container 100 is made of transparent or semitransparent plastic material so that the inside of the aseptic culture container 100 can observe the growth of the tissue culture body. In addition, preferably, the inner surface of the container body 10 is preferably subjected to antibacterial treatment. The inner surface of the container body 10 may be coated with an inorganic antibacterial agent.

The shape of the aseptic culture container 100 is not limited and may be a cylindrical shape or a polygonal shape. Also, as shown in Fig. 1, a part of the side surface may be recessed into the inside so as to facilitate gripping.

As shown in FIG. 2, an air inlet 20, a check valve 30 and a porous sparger 40 are systematically installed in a lower space of the bottom surface of the container body 10. The air inlet 20 is opened to one side of the sterile culture container 100. A backflow prevention valve 30 is provided in succession to the air inlet 20. A porous sparger 40 having a plurality of holes formed on the surface thereof is installed in a shape protruding in a hemispherical shape on the bottom surface of the container body 10. The plurality of holes formed on the surface of the porous sparger 40 may be formed only on the portion protruding into the container or may be formed on the entire surface of the porous sparger 40.

Through the air inlet 20, sterilized air is injected from the outside as described later. The injected air passes through a check valve 30 connected to the air inlet 20. Although the sterilized air injected from the outside by the check valve 30 passes, the culture liquid inside the container, which can be partially flowed out through the holes of the porous sparger 40, is prevented from flowing out because the flow thereof is suppressed. The air that has passed through the check valve 30 is uniformly sprayed through a plurality of holes of a porous sparger protruding in a hemispherical shape to supply oxygen to the plant tissue culture, Stir in the vessel. At this time, a semi-spherical porous sparger cover 41 corresponding to the external shape of the sparger is provided outside the protruding porous sparger 40, so that the holes of the porous sparger are clogged due to foreign substances, It is preferable to prevent foreign matter from being mixed into the culture liquid through the sparger. At this time, it is preferable that holes are alternately formed in the cover 41 of the porous sparger, as shown in FIG. 2, with holes formed on the surface of the porous sparger 40.

At this time, preferably, the porous sparger 40 is formed so as to protrude into the container at a position eccentric from the center of the bottom of the container body. When air is injected from the center of the bottom of the container to agitate the culture medium and the tissue culture medium, the tissue culture body adheres to the wall of the container or accumulates in a part of the space, so that uniform nutrition and oxygen supply can not be achieved .

Further, more preferably, in the porous sparger 40, it is preferable that a plurality of holes, which are paths of outside air, are formed in different sizes. According to one embodiment, the diameter of the hole is inversely proportional to the distance from the holes formed in the surface of the hemispherical porous sparger protruding into the container to the inner wall surface of the container in the direction in which air is injected . If the diameter of the hole is set smaller as the distance to the inner wall of the vessel is increased in the direction in which the air is injected, air is injected at a higher pressure, so that even stirring can be performed to supply uniform oxygen and nutrients to the plant tissue culture . In the case where the porous sparger cover 41 is formed, the diameters of the plurality of holes formed in the cover may be different from each other in the above-described manner.

An upper lid 50 is provided on the upper part of the container body 10. The upper lid 50 is formed inside the container body 10 to inject the culture liquid and the plant tissue culture to be cultured, and to collect the cultured plant tissue culture. The culture medium and the plant tissue culture are injected into the container under the sterilized condition before it is provided to cultivate the plant tissue culture such as the wild ginseng culture at home. And then sealed with an upper cover 50 to be supplied to the culture. After the cultivation at home is completed, the cultured plant tissue culture is harvested again by opening it. At home, it can be opened only after the culture is finished, so sterilization can be maintained during cultivation.

Since the gas is generated during the tissue culture of the plant, the gas inside the container must be discharged to the outside. For this purpose, an exhaust port (60) is formed on the upper surface of the upper lid (50). At this time, it is preferable that the exhaust port is formed to be opened to the side of the container, as shown in FIG. 1, in order to prevent contamination from contaminants falling vertically. Preferably, an air filter 61 is installed in the exhaust port to prevent external air from entering through the exhaust port 60 and contamination. There is no limitation on the type thereof, and a fine filter having a diameter larger than that of the bacteria elimination filter 71 of the present invention can be used.

3, an air pump 70, a sterilizing filter 71 for sterilizing the air supplied from the air pump 70, a sterilizing filter 71 for sterilizing the air supplied from the air pump 70, A control valve 72 for regulating the amount and pressure of the air supplied from the air pump 70 and an air inlet 20 of the aseptic culture container 100 of the plant tissue culture to sterilize the air in the aseptic culture container 100 An air supply device 200 composed of a connection cap 73 for injecting the air into the inside of the chamber; And

(20) formed at one side of the lower portion of the container body (10) for containing sterilized air, and air introduced from the air inlet (20) A backflow prevention valve 30 for preventing the leakage of the culture liquid contained in the container body 10 while being protruded in the form of a hemisphere on the inner bottom surface of the container body 10, A porous sparger 40 for spraying the air injected through the tubular body 20 into the container body to supply air to the plant tissue culture body and agitating the culture liquid and the plant tissue culture body, And an exhaust port 60 protruding from the upper surface of the upper lid 50 and discharging the gas generated inside the vessel body 10 to the outside, 100) Water cultures sterile tissue culture system is provided.

As shown in FIG. 3, the air supply device 200 supplies sterilized air to the plant tissue culture aseptic culture container 100. According to one embodiment of the present invention, the air supplied from the air pump 70 is sterilized while passing through the sterilizing filter 71. As the bacteria elimination filter, a fine filter of 0.2 mu m can be used. The supply amount and the supply pressure of the air that has passed through the sterilizing filter 71 are adjusted using the regulating valve 72 and supplied to the culture container 100. The sterilized air whose amount and pressure are adjusted using the control valve 72 moves along the air tube and is transported through the connection cap 73 connected to the air inlet 20 of the aseptic culture container 100 of the plant tissue culture do. As shown in FIG. 3, according to one embodiment of the present invention, air can be injected along three branched paths, so that simultaneous tissue culture through three culture vessels is possible.

4, the connection cap 73 of the air supply device 200 is connected to the air inlet 20 of the aseptic culture container 100 of the plant tissue culture to supply sterilized air. The air supply device 200 and the aseptic culture container 100 are integrated so that the user can connect the connection cap 73 of the air supply device 200 to the air inlet of the aseptic culture container 100 The plant tissue culture can be carried out through a simple operation of connecting to the plant 20. When the cultivation is completed, the connection site is released and the culture container having the other conditions is replaced, thereby allowing the tissue culture to be repeated several times without further sterilization.

According to another aspect of the present invention, there is provided an air conditioner comprising: an incubator lower body (300) having the air supply device (200) installed therein; And

And an incubator main body (400) in which the plant tissue culture aseptic culture container (100) is installed, wherein the plant tissue culture aseptic culture device (100)

A cultivation support support 410 is formed on the bottom surface of the incubator main body 400 to be rotatably coupled to the aseptic culture support vessel 100 and is connected to the front surface of the incubator main body 400 A sterilized culture of a domestic plant tissue culture in which an internal confirmation window 421, a display and operation unit 422 and a door unit 423 are formed and an internal illumination device 430 is formed on the upper surface of the incubator main body 400 Device is provided.

5 and 6 are views showing an incubator in which three aseptic culture containers for plant tissue cultures can be installed and cultivated according to an embodiment of the present invention, and the door portions of the incubator are in a closed state and an open state, respectively.

As shown in FIGS. 5 and 6, according to an embodiment of the present invention, an incubator lower body 300 in which the air supply device 200 is installed; And

And an incubator main body 400 in which the plant tissue culture aseptic culture container 100 is installed, is provided.

As shown in FIG. 6, a cultivation support stand 410 is formed on the bottom surface of the incubator main body 400, to which the aseptic cultivation container 100 of the plant tissue culture is coupled and rotatably installed . A hole (not shown) through which a connection cap 73 for supplying air to be delivered from the air supply device 200 built in the lower body 300 of the incubator passes is formed on the bottom surface of the culture container support stand 410. Since the culture container support table 410 is rotatably installed, the user can observe each culture container during the culture using a plurality of culture containers, or open the door portion 423 when replacing the culture container after completion of culture, It is convenient to rotate and work.

Inside the incubator body, a temperature sensor for sensing the internal temperature and a fan for controlling the temperature of the exhaust and the temperature may be installed. As shown in FIG. 5, an internal confirmation window 421 is installed in a front portion of the incubator main body. This is for observing the inside without opening the door part 423. The internal confirmation window 421 is preferably formed of a material having a transmittance of 60 to 70%. In addition, a display display and operation unit 422 is installed on the front surface of the incubator main body 400. The display and operation unit 422 displays the temperature, incubation time, air pump operation, lighting device operation status, and the like inside the incubator main body 400. The user controls a microcomputer (not shown) installed in the incubator through the operation unit These conditions can be manipulated. In addition, a door portion 423 is formed on the front portion of the incubator main body. The user can conveniently observe and replace the culture container through the opening and closing of the door part. An internal illumination device 430 is installed on the upper surface of the incubator main body 400. The inside of the incubator main body is illuminated through the illumination device 430 so that observation and operation are convenient even in a dark environment, and light necessary for the plant tissue culture body inside the culture container is supplied. On the other hand, it is preferable that the illumination condition can be changed through the operation of the display / display unit 422 because the intensity of light required for growth and color temperature may vary depending on the plant tissue to be cultivated. For example, when growing root tissue, light should be blocked, and light should be provided when growing above ground. For this purpose, it is preferable that an LED lighting device capable of adjusting the color temperature or intensity of light is used as the lighting device 430.

The incubator is not limited in shape, and may be formed into a cylindrical shape as shown in Figs. 5 and 6. In addition, a mood lamp for indoor illumination may be further provided on an upper outer surface of the incubator. The plant tissue culture aseptic culturing apparatus of the present invention can be used for tissue culturing of all plant rootlets, culture seedlings, callus, whole plants, tubers and the like.

The present invention relates to a container for an aseptic culture vessel, which comprises a container body 20, an air inlet 30, a backflow prevention valve 40, a porous sparger 41, a porous sparger cover 50, an upper cover 60,
70: air pump 71: filter for removing bacteria 72: regulating valve 73: connecting cap 200: air supply device
300: incubator lower body 400: incubator main body 410: culture vessel support table 421: internal confirmation window 422: display display and manipulation part 423: door part 430:

Claims (19)

(20) formed at one side of the lower portion of the container body (10) for containing sterilized air, and air introduced from the air inlet (20) A backflow prevention valve 30 for preventing the leakage of the culture liquid contained in the container body 10, a protrusion formed in a hemisphere shape on the inner bottom surface of the container body 10, A porous sparger 40 for spraying air injected through the tubular body 20 into the container body 10 to supply air to the plant tissue culture body and agitating the culture liquid and the plant tissue culture body, And an exhaust port 60 protruding from the upper surface of the upper lid 50 and discharging the gas generated inside the vessel body 10 to the outside, Culture vessel. The aseptic culture container according to claim 1, wherein the body of the aseptic culture container (100) is made of transparent or semitransparent plastic. 2. The aseptic culture vessel of claim 1, wherein the inner surface of the container body (10) is treated with an antibacterial agent. 2. The aseptic culture vessel for plant tissue culture as claimed in claim 1, wherein the porous sparger (40) is protruded from the center of the bottom of the container body (10) at an eccentric position. 2. The plant as defined in claim 1, wherein a hemispherical porous sparger cover (41) corresponding to the outer shape of the sparger is additionally provided outside the protruding porous sparger (40). . 2. The plant as claimed in claim 1, wherein a plurality of holes formed in the surface of the porous sparger (40) to serve as a passage for external air are formed in different sizes. . 7. The porous sparger (40) according to claim 6, wherein the plurality of holes formed on the surface of the hemispherical porous sparger (40) protruding into the inside of the container is a distance from a hole Wherein the diameter of the hole is formed to be in inverse proportion to the diameter of the aseptic culture container. The aseptic culture container according to claim 1, wherein the air outlet (60) is provided with an air filter (61) for preventing contamination from the outside. The aseptic culture vessel of claim 1, wherein the exhaust port (60) is formed to face the side of the container body (10) in order to prevent contamination due to contaminants falling vertically. The sterile culture container (100) according to claim 1, wherein the aseptic culture container (100) is formed in a cylindrical shape in which a part of the side is recessed inwardly so as to facilitate circular, polygonal, or grasping. . A sterilizing filter 71 for sterilizing the air supplied from the air pump 70; a regulating valve 72 for regulating the amount and pressure of the air supplied from the air pump 70; An air supply device 200 connected to the air inlet 20 of the aseptic culture container 100 of the tissue culture body and having a connection cap 73 for injecting the sterilized air into the sterile culture container 100; And
(20) formed at one side of the lower portion of the container body (10) for containing sterilized air, and air introduced from the air inlet (20) A backflow prevention valve 30 for preventing the leakage of the culture liquid contained in the container body 10 while being protruded in the form of a hemisphere on the inner bottom surface of the container body 10, A porous sparger 40 for spraying the air injected through the tubular body 20 into the container body to supply air to the plant tissue culture body and agitating the culture liquid and the plant tissue culture body, And an exhaust port 60 protruding from the upper surface of the upper lid 50 and discharging the gas generated inside the vessel body 10 to the outside, 100) Plant tissue culture aseptic culture system. An incubator lower body 300 in which the air supply device 200 of claim 11 is installed; And
A plant culture system aseptically cultivated by a plant for culturing a plant tissue comprising the culture apparatus main body (400) in which the aseptic culture culture vessel (100) of any one of claims 1 to 10 is installed,
A cultivation support support 410 is formed on the bottom surface of the incubator main body 400 to be rotatably coupled to the aseptic culture support vessel 100 and is connected to the front surface of the incubator main body 400 And an interior lighting device 430 is formed on the upper surface of the incubator main body 400. The indoor lighting device 430 is provided on the upper surface of the incubator main body 400, Tissue culture aseptic culture device. 13. The apparatus for aseptic cultivation of a domestic plant tissue culture system according to claim 12, wherein three aseptic culture vessels (100) are installed in the main body (400) of the incubator. 13. The apparatus for aseptic cultivation of a domestic plant tissue culture according to claim 12, wherein the incubator main body (400) is formed in a cylindrical or polygonal shape. 13. The apparatus for aseptic cultivation of a domestic plant tissue culture system according to claim 12, wherein the illumination device (430) is an LED lighting device capable of adjusting the color temperature and intensity of light. 13. The apparatus for aseptic cultivation of a plant tissue culture according to claim 12, wherein a temperature sensor and a fan for sensing the temperature of the inside of the incubator main body (400) are additionally provided. 13. The apparatus for aseptic cultivation of a domestic plant tissue culture system according to claim 12, wherein a mood lamp for indoor lighting is additionally provided on an upper outer surface of the incubator main body (400). 13. The aseptic culturing apparatus for a plant tissue culture as set forth in claim 12, wherein the aseptic culture apparatus for plant tissue cultures is used for tissue culturing of plant root, culture seed, callus, whole plant, tuber, etc. 13. The apparatus for aseptic cultivation of a domestic plant tissue culture system according to claim 12, wherein the interior identification window (421) has a light transmittance of 60 to 70%.

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