KR20160085401A - A feed additive including immunity induced silkworm and manufacturing method thereof - Google Patents

Abstract

Disclosed are a feed composition containing silkworms with increased antibacterial peptides, and a manufacturing method thereof. The manufacturing method of a feed composition containing silkworms with increased antibacterial peptides comprises the following steps: preparing silkworms inoculated with an immunity inducer; and manufacturing the feed composition using the silkworms. The feed composition is fed to an animal., and the result of feeding shows that the feed composition has various effects such as productivity enhancement, health maintenance, inhibition of harmful enteric bacteria, immunity control, etc. The feed composition shows an equal or excellent result in comparison with a growth promoting antibiotic, and thus can be used as a novel antibiotic substitute in the future.

Description

[0001] The present invention relates to a feed composition containing an immunologically-induced silkworm,

The present invention relates to a feed composition containing an immunologically-induced silkworm and a method of preparing the same.

Recently, as the consumer's interest in health has increased due to the improvement of the economic level, the qualitative aspect of livestock products has been emphasized more than the quantitative aspect, and improvement of quality and safety of livestock products has become more important for national health as well as securing competitiveness of domestic livestock industry . In addition, sustainable environmentally friendly circulation-type livestock becomes more important, and it is necessary to highlight the positive aspect of livestock industry through the use of idle agricultural products and domestic resources. As a result, researches for producing high quality safe livestock products utilizing domestic resources have been actively conducted.

Domestic silkworms abruptly declined to the peak of 1976. Since 1995, silkworm cocoon production has almost ceased and now it has been converted into production of functional silkworms such as dried silkworms and Chinese caterpillar fungus. However, domestic silkworm breeding / production technology and potential are accumulated compared to other insects, so it is possible to produce and supply stable when securing stable production market and developing high value-added products. Korean Patent Publication No. 10-2000-0025517 discloses a technique for producing a feed composition using silkworm excrement.

However, in the field of livestock, there are not many examples and researches on application of antimicrobial peptides derived from silkworm nematode to livestock.

The above-described background technology is technical information that the inventor holds for the derivation of the present invention or acquired in the process of deriving the present invention, and can not necessarily be a known technology disclosed to the general public prior to the filing of the present invention.

       The present invention provides a feed composition containing silkworm-strengthened antimicrobial peptides having various effects such as productivity improvement, health maintenance, suppression of intestinal harmful bacteria, immunity control, and a preparation method thereof.

The technical problems other than the present invention can be easily understood from the following description.

       According to an aspect of the present invention, there is provided a method of preparing a silkworm comprising: preparing a silkworm injected with an immunity inducing substance; And a step of adding the silkworms to produce a feed composition, wherein the silkworm peptide-enriched silkworm-containing feed composition is provided.

       Here, the feed composition may further comprise an excipient, and 1 to 50 parts by weight of the silkworm and 50 to 99 parts by weight of the excipient may be added to 100 parts by weight of the entire feed composition.

       In addition, the excipient may include any one or more of horse chestnut, limestone, ground cornstarch, corn gluten feed, and soybean.

       Here, the silkworm may be added to the feed composition in powder or liquid form, and the feed composition may be in powder or pellet form.

       Here, preparing the silkworm may further include drying the silkworm using microwaves.

       Here, the feed composition may be a feed for animals of one or more of chicken, duck, goose, pheasant, pig, cow, goat, dog and cat.

In addition, the silkworm may be added to the feed at a rate of 0.005 to 0.05% of the mass of the whole feed composition.

Other aspects, features, and advantages will become apparent from the following drawings, claims, and detailed description of the invention.

The feed composition containing the antimicrobial peptide-enriched silkworm peptide, which was developed in the present invention, was added to chicken to give various effects such as improvement of productivity, maintenance of health, inhibition of intestinal bacteria, immunity control, It can be used as a new antibiotic substitute in the future with excellent results.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart illustrating a method for preparing a feed composition containing silkworm peptide-enriched silkworms according to an embodiment of the present invention. FIG.
FIG. 2 is a graph showing changes in weight of a silkworm powder enriched with an antimicrobial peptide according to an embodiment of the present invention by temperature and time during manufacture by hot air drying.
FIG. 3 is a graph showing changes in weight of a silkworm powder enriched with an antimicrobial peptide according to an embodiment of the present invention when the powder was produced by microwave drying.
Figure 4 is a comparison of silkworm powder antibacterial activity according to the drying method of silkworm powder enriched with an antibacterial peptide according to an embodiment of the present invention.

       While the invention is susceptible to various modifications and alternative forms, specific embodiments thereof are shown by way of example in the drawings and will herein be described in detail. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

        Terms including ordinals, such as first, second, etc., may be used to describe various elements, but the elements are not limited to these terms. The terms are used only for the purpose of distinguishing one component from another.

        It is to be understood that when an element is referred to as being "connected" or "connected" to another element, it may be directly connected or connected to the other element, .

        The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

        The term " part, "" module," " means, "or the like, which is described in the specification, refers to a unit for processing at least one function or operation and may be implemented by hardware or software or a combination of hardware and software .

In the following description of the present invention with reference to the accompanying drawings, the same components are denoted by the same reference numerals regardless of the reference numerals, and redundant explanations thereof will be omitted. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description, well-known functions or constructions are not described in detail since they would obscure the invention in unnecessary detail.

        1 is a flow chart showing a method for producing a feed composition containing silkworm peptide-enriched silkworm according to an embodiment of the present invention. The feed composition of this embodiment can be animal feed such as chicken, duck, goose, pheasant, pig, cattle, goat, dog and cat.

     The silkworm has various physiologically active substances such as cecropin, γ-aminobutyric acid (GABA), and rutin, and it has antibacterial, antioxidant, liver and kidney function improvement, toxicity prevention, And a variety of physiological effects such as nervous stability. Especially, antimicrobial, antioxidant, antiviral, insecticidal, cell regeneration and immunomodulating effects of the antimicrobial peptide component of silkworm, secrophin and enbocin, are great, and their industrial utility value is very high.

        In addition, the feed composition according to the present embodiment may be implemented in the form of a powder or a pellet having a predetermined volume, and the silkworm may be added to the feed composition in powder or liquid form. Hereinafter, the case where silkworm is powdered and added to the feed composition will be mainly described.

        Each step to be described below can be performed as a feed composition manufacturing apparatus. It is needless to say that the processes described in each step need not necessarily be performed in a time series order, and the process may be included in the scope of the present invention if the order of the steps is changed but the gist of the present invention is satisfied.

        In step S110, the silkworm injected with the immunity inducing substance is pulverized. Here, Lactobacillus plantarum, a cell wall structure similar to pathogenic Gram-positive bacteria, was cultured for the development of injections for silkworm immunity induction. That is, L. plantarum n-butanol extract (pLTA) containing L. plantarum live cells, L. plantarum cell wall extract (pCW) and lipoteichoic acid (LTA) Were used for selection.

        L. plantarum cell wall (pCW) was prepared according to the following method. First, the lyophilized strain was dissolved in sterilized distilled water, heated at 100 ° C for 20 minutes, and centrifuged at 1,500g for 10 minutes to remove unbroken bacteria. The supernatant was centrifuged again at 6,500g for 30 minutes. The precipitated cell wall was collected, washed three times with distilled water, and lyophilized, and then used for LTA extraction and silkworm immunity induction test.

        In addition, L. plantarum LTA extract (pLTA) was prepared according to the following method. Extracts containing LTA were prepared using n-butanol extraction from L. plantarum cell wells. The lyophilized cell wells were dissolved in 0.1 M sodium citrate buffer (pH 4.7), pulverized with a supersonic crusher, and the same amount of n-butanol was added thereto, followed by stirring at room temperature for 30 minutes. After extraction, centrifugation was carried out at 10,000 rpm for 30 min. The supernatant was recovered, dialyzed with evaporate and sterilized distilled water, and lyophilized. The extract containing lyophilized LTA was weighed and then dissolved in sterile saline to be used for silkworm immunity induction test.

       2, 50 μl aqueous solution containing various concentrations of L. plantarum live cells, cell wall extract (pCW) and LTA extract (pLTA) was inoculated in a 5-day-old 5-day silkworm The antibacterial activity against Escherichia coli was examined by collecting body silkworms with time (6 hours, 10 hours, 22 hours).

        Antibacterial activity analysis showed a very low antimicrobial activity in the case of inoculation with live bacteria. In case of cell-injected immunization induction, high mortality was observed at 0.5 mg / But showed low antimicrobial activity. The highest antimicrobial activity was observed after 10 hours at 5 ~ 10mg / ml injection concentration of pLTA injection. Therefore, L. plantarum LTA extract (pLTA) was the most effective inducer for silkworm immunity induction.

        3, the antimicrobial activity of the selected L. plantarum LTA extract (pLTA) according to inoculation concentration and induction time was examined to establish optimal silkworm immunity induction conditions. 10, 5, 1, and 0.5 mg / ml. The silkworms were harvested at 6, 10, 18, and 22 hours after immune induction and used for the antimicrobial activity test for E. coli. The antimicrobial activity showed the highest antimicrobial activity at the concentration of 5 mg / ml and 1 mg / ml. Therefore, the injection concentration for silkworm immunity induction can be determined to be 1 mg / ml, and the immunity induction time can be determined to be more than 10 hours but less than 22 hours.

        Immune inducers can be injected into growing silkworm larvae. This embodiment may include a device for injecting an immunoinducing substance into silkworm larvae. For example, the infusion device may include a larval support, which is the portion of the larva where the injection is to be injected, an injection infusion section that injects the infusion containing the immunoinducing substance into the larva placed on the larval support, A caterpillar inactivating section for inserting the cryoprotectant into the cryoprotecting section, and a larva storing section which is a means for storing the caterpillar into which the injecting liquid is injected by the injecting liquid injecting section and has been injected.

        Here, the caterpillar deactivating portion can be a water tank containing cold water, and when the caterpillar is contained in cold water, it is inactivated such as instantaneously stunning, so that the caterpillar supporting portion can easily support the dull caterpillar.

In step S112, the silkworm injected with the immunity inducing substance may be dried by a hot air drying method. In the hot air drying method, silkworm is dried by using hot air having a predetermined temperature.

        Further, according to another embodiment, in step S114, the silkworm injected with the immunity inducing substance may be dried by the microwave drying method.

A silkworm (1.8kg) was charged by the temperature of the hot air (60 ° C, 70 ° C, 80 ° C, 90 ° C) and microwave oven to measure the mass every hour. After the final time and weight were measured, the difference of general components by temperature was analyzed (analysis items: moisture, ash, crude fat, crude protein, crude fiber) and antibacterial test.

        Referring to FIG. 4, silkworm states by temperature (60 ° C, 70 ° C, 80 ° C and 90 ° C) by hot air drying were compared. When the initial weight of 1800 g of silkworm was subjected to hot air drying, it took about 34 hours at 60 ° C for equilibrium point, 26 hours at 70 ° C, 25 hours at 80 ° C and 24 hours at 90 ° C. When the equilibrium point was reached, the weight was 319.9 g at 60 캜, 329 g at 70 캜, 323 g at 80 캜, and 315 g at 90 캜. This indicates that when the equilibrium point is reached at a total weight of 1800 g, the average weight is 17%.

        Referring to FIG. 5, when the microwave is used, the point at which the equilibrium point appears is about 40 minutes. In addition, the general components of dried silkworms using microwave ovens and hot air drying by temperature were analyzed and the difference was not significant. Therefore, the most efficient drying method in a short period of time is analyzed as a microwave-assisted method.

        As shown in Table 1 and Table 2, after a certain period of time, there was no significant difference between the composition ratios of the general components of dried silkworms (significant differences). Considering the time and drying cost of the hot air drying of the silkworm, it can be seen that the silkworm drying at 90 ° C is more economical than the lower temperature of 60 ° C. In general, Efficient. Analysis of the components after moisture correction shows similar results.

Name of sample moisture(%) Ashes (%) Crude fat (%) Crude protein (%) Crude fiber (%) Microwave 5.02 5.67 14.71 68.02 4.85 60 degrees - drying 4.67 5.70 14.58 68.99 4.12 70 degrees - drying 7.54 5.80 14.19 66.20 4.32 80 degrees - drying 4.01 6.12 14.08 69.96 4.38 90 degrees - drying 2.53 6.20 14.43 70.07 4.52

Name of sample moisture(%) Ashes (%) Crude fat (%) Crude protein (%) Crude fiber (%) Microwave - 5.97 15.64 71.62 5.11 60 degrees - drying - 5.98 15.29 72.37 4.32 70 degrees - drying - 6.27 15.14 71.60 4.67 80 degrees - drying - 6.38 15.04 72.88 4.56 90 degrees - drying - 6.36 15.41 71.89 4.64

Then, 5 ml of 0.3% acetic acid was added to 0.5 g of each powder sample. The mixture was stirred at room temperature for 1 hour, centrifuged at 10,000 rpm at 4 ° C for 20 minutes, and the supernatant was separated. The supernatant was concentrated to 1 ml, Respectively.

        Antimicrobial activity assay was carried out by using radioactive diffusivity analysis (RDA). The antimicrobial activity of E. coli ML35 was compared with that of the inhibition zone indicating inhibition of pathogen growth.

        6, the antimicrobial activity of the samples dried by microwave (MW) was the highest, and the samples subjected to the hot air drying showed different results depending on the temperature.

        According to the above description, when hot air is used for drying the silkworm, the application of the silkworm powder at a low temperature is required to increase the antibacterial activity of the silkworm powder, but the drying time may increase and the drying cost may increase. However, when the microwave is used, the silkworm powder has a high antimicrobial activity and the drying time is greatly reduced as compared with the hot wind. Therefore, it is analyzed that the microwave - drying silkworm is more efficient than the hot wind drying.

In step S120, the powdered silkworm is added to the feed to produce the feed composition. In this case, the silkworm powder may be mixed with a carrier, a substance used for preliminary blending to add the trace materials to the feed. Here, the excipient is a component added for the purpose of imparting a suitable hardness or shape to the medicament, or to make it easy to handle with a certain volume and weight when the amount of the main agent is small, such as horse chestnut, limestone, Corn cob, corn gluten feed, soybean mill run, and the like.

        Here, 1 to 50 parts by weight of silkworm and 50 to 99 parts by weight of excipient may be included relative to 100 parts by weight of the whole feed composition.

Hereinafter, a method for producing a feed composition containing silkworm-enhanced silkworm peptide was described. Hereinafter, with reference to the accompanying drawings, the effect of the feed composition containing the silkworm-containing feed composition containing the antimicrobial peptide according to the present invention The results are shown in Fig. It goes without saying that the present invention is not limited to these embodiments.

In order to test the performance of the feed composition according to the present example, feeding of a silkworm-derived antimicrobial peptide-containing feed composition to chicken was tested. To investigate the effect of addition of the silkworm powder - containing feed composition developed for chicken on the 1 - day - old broiler chickens, a 5 - week specification test was conducted. In Comparative Example 1, no antibiotics were added, in Comparative Example 2, antibiotics (aviramycin 10 ppm + salinomycin 60 ppm), and in Examples 1, 2, and 3 in which general silkworm powder was added at a level of 0.01, 0.05, 5, 6, 7 and 8 in which silkworm powder inducing the production of an antimicrobial peptide was injected at a level of 0.01, 0.05, 0.10 or 0.50% by injecting an immunopotentiating substance. All of the examples did not include feed antibiotics and anticoccidials.

division Antibiotic Processing contents Comparative Example 1 × No additives Comparative Example 2 ○ Feed supplemented with antibiotics (avilamycin 10ppm + salinomycin 60ppn) Example 1 × Feed of 0.01% by weight of general silkworm powder Example 2 × Feed of 0.05% by weight of general silkworm powder Example 3 × Feeding 0.10% by weight of general silk powder Example 4 × Feeding 0.50% by weight of general silk powder Example 5 × 0.01% by weight of immuno-inducing silkworm powder Example 6 × Immune-induced silkworm powder 0.05 wt% Example 7 × Immune-inducing silkworm Powder 0.10% by weight Added feed Example 8 × 0.50% by weight of immunostimulatory silkworm powder

Table 4 compares broiler productivity. In all of the examples, the body weight and weight gain at the end of 5 weeks increased by 0.95 to 3.54% as compared to Comparative Example 1. [ In particular, Examples 5, 6, 7 and 8, in which the antimicrobial peptide was overexpressed by immunization induction, showed a higher weight gain effect than Examples 1, 2, 3 and 4 in which the general silkworm powder was fed. The feed conversion ratio was also improved by 2.82-5.08% in all the examples in which the silkworm powder was fed as compared with Comparative Example 1. [ Accordingly, the feed composition containing the silkworm-derived antimicrobial peptide according to the present invention has an excellent productivity improvement effect by improving the amount of feed and the feed conversion ratio. In terms of productivity, the optimum addition level is 0.01-0.50% for general silkworm powder, 0.01 To about 0.05%.

Comparative Example 1 Comparative Example 2 Implementation 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Starting weight (g) 44.4 44.0 44.1 44.1 44.1 44.5 44.3 44.4 44.4 44.1 Finished weight (g) 2202.0 2416.4 2229.4 2222.9 2227.9 2240.6 2280.0 2254.2 2273.6 2225.9 Weight gain (g) Electricity (0 to 21 days) 917.1 995.6 925.7 919.9 927.7 953.5 964.5 964.4 965.1 964.5 The latter (21 ~ 35) 1284.9 1420.8 1303.8 1303.0 1300.2 1287.1 1315.5 1348.9 1314.0 1287.8 Before (0 ~ 35 days) 2157.6 2372.5 2185.3 2178.8 2183.8 2196.2 2235.7 2209.9 2229.3 2181.9 Feed intake (g) Electricity (0 to 21 days) 1268.2 1316.3 1088.2 1094.1 1101.9 1161.2 1131.7 1076.5 1146.8 1177.0 The latter (21 ~ 35) 2587.3 2813.7 2744.7 2701.8 2635.3 2664.4 2680.9 2733.3 2687.0 2654.5 Before (0 ~ 35 days) 3812.7 4057.0 3703.6 3693.3 3670.1 3773.3 3744.5 3681.4 3765.0 3682.5 Feed rate Electricity (0 to 21 days) 1.45 1.38 1.23 1.25 1.25 1.27 1.23 1.23 1.34 1.28 The latter (21 ~ 35) 2.02 1.98 2.11 2.08 2.03 2.07 2.04 2.06 2.13 2.05 Before (0 ~ 35 days) 1.77 1.71 1.70 1.70 1.68 1.72 1.68 1.68 1.69 1.69

        In order to confirm the biosafety of silkworm and immunity inducing silkworm powder on broiler chickens, the relative weight and biochemical composition of organs were investigated. As a result, it was found that, when fed in broiler chickens for 0.01 to 0.50% And liver and damage index in the blood.

Table 5 compares the relative weights of the major organs of broilers. The effects of dietary supplementation of common silkworm powder and immunostimulatory silkworm powder on the development of major organs (liver, spleen, pancreas, kidney, and follicle) of broiler chickens were examined. As a result, And that the addition of normal silkworm powder and immunostimulatory silkworm powder to the broiler chickens at the level of 0.01 ~ 0.50% in the feed for 5 weeks did not affect the major organ development.

Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 ---------- g / 100g live weight ---------- liver 2.12 1.97 1.92 1.85 2.02 1.90 1.90 2.16 1.83 1.85 spleen 0.14 0.10 0.10 0.12 0.11 0.10 0.11 0.12 0.12 0.11 Pancreas 0.22 0.23 0.21 0.22 0.20 0.21 0.25 0.23 0.26 0.23 kidney 0.35 0.34 0.35 0.37 0.36 0.38 0.33 0.35 0.34 0.36 F Sack 0.09 0.08 0.08 0.07 0.06 0.07 0.07 0.07 0.06 0.06

Table 6 compares the biochemical composition in the blood. (BUN, creatinine, total protein, albumin), which is used as an index to determine toxicity of feed additive, Globulin, and aspartate aminotransferase (AST), the levels of AST and ALT, which are liver and kidney damage indexes, were decreased in the Examples in comparison with Comparative Example 1. Other elements such as urea nitrogen, creatinine, total protein, albumin, The results showed that the feed composition containing the antibacterial peptide derived from silkworm reduced the cell and tissue damage through antioxidant and antiinflammatory action and regulated the bioimmune response and did not show any toxicity or side effects on the livestock, It can be used as an additive.

Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Elemental failure (mg / dL) 1.25 1.19 1.12 1.11 1.09 1.18 1.22 1.17 1.13 1.21 Creatinine (mg / dL) 0.27 0.26 0.28 0.27 0.27 0.26 0.28 0.26 0.26 0.29 Total protein (mg / dL) 3.36 3.43 3.33 3.21 3.21 3.43 3.27 3.38 3.49 3.48 Albumin (mg / dL) 1.58 1.60 1.49 1.50 1.47 1.54 1.48 1.53 1.57 1.59 Globulin (mg / dL) 1.78 1.83 1.84 1.71 1.74 1.89 1.79 1.84 1.92 1.89 Albumin / globulin 0.94 0.85 0.79 0.94 0.84 0.88 0.74 0.89 0.78 0.88 AST (U / L) 299.7 274.3 271.2 271.3 276.2 269.9 268.4 273.2 265.1 257.3 ALT (U / L) 3.89 3.91 3.33 3.89 3.11 3.44 3.11 3.11 2.78 3.11

Table 7 compares the total microorganism changes in the cecum. It was confirmed that Escherichia coli and Salmonella, which are pathogenic bacteria in the intestines, were reduced in all the examples in which feed composition containing common silkworm powder and immunity-inducing silkworm powder was added, as compared with Comparative Example 1. In addition, it was confirmed that lactic acid-producing bacteria, which are beneficial bacteria in the intestines, are not affected, thus positively affecting total intestinal microbial stability.

Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 ---------- log10 cfu / g ---------- Seed bacteria 8.511 8.118 8.385 8.178 8.212 8.158 8.158 8.179 8.064 8.015 Escherichia coli 6.165 5.817 5.987 5.959 5.988 6.014 5.898 5.825 5.878 5.810 Salmonella 4.416 3.345 4.345 4.254 4.138 4.225 3.985 3.725 3.578 3.661 Lactic acid-producing bacteria 7.405 7.152 7.396 7.408 7.265 7.521 7.358 7.452 7.257 7.402

Table 8 compares the antioxidant and stress-related factors in the blood. Total antioxidative activities of blood were higher than those of the comparative examples, and total antioxidant activity tended to increase as the level of silkworm powder was increased. Analysis of the content of cortisol, which is known as a representative stress hormone, results in a decrease in all the examples compared to Comparative Example 1. Cortisol, which is secreted during stress, disease infections, and inflammatory responses, can affect basal metabolic rate, protein synthesis and degradation, immune response, and body homeostasis, which can negatively impact productivity in the long term as well as in animal health. It was found that silkworm powder has a positive effect on productivity as well as animal health by lowering cortisol content in blood similar to antibiotics and stress control effect.

Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 End antioxidant activity
(mM TEAC / mL) 48.97 50.84 50.27 49.03 50.01 50.39 49.65 49.88 50.72 51.53 Cortisol (ng / mL) 12.65 6.22 8.81 7.34 8.04 8.02 7.95 7.36 6.96 7.33

Table 9 shows the effect of the addition of silkworm powder on the composition of leukocyte in broiler chickens. The ratio of polynuclear core / lymphocyte used as a stress index as well as the heterophil which increases in acute infection or inflammation reaction is significantly higher than that of Comparative Example 1 in which the addition of general silkworm powder and immunostimulatory silkworm powder is added , Respectively. The feed composition containing the silkworm-derived antimicrobial peptide according to the present invention is believed to suppress the bio-immune response to these by inhibiting the growth of harmful microorganisms such as Escherichia coli and Salmonella in the intestinal tract and their toxin secretion.

Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Leukocytes (K / l) 23.04 20.58 23.12 23.72 22.46 24.36 24.36 26.64 24.34 29.42 The polynuclear spheres (K / L) 8.16 7.05 7.69 7.93 7.09 7.63 7.63 7.82 7.10 9.10 Lymphocytes (K / l) 11.94 11.45 11.88 12.16 11.13 12.74 12.74 13.48 12.83 15.22 Polynuclear cavity / lymphocyte 0.68 0.62 0.65 0.65 0.64 0.60 0.60 0.58 0.55 0.60

        Other specific examples of the feed composition containing the silkworm-enhanced silkworm peptide according to the embodiment of the present invention and the specific feed composition, the manufacturing apparatus, and the like for the method of preparing the silkworm peptide composition are described in the description of the present invention, It will be omitted.

         In the above description, the respective components and / or functions described in the embodiments may be combined and combined, and those skilled in the art will understand that the present invention It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.

Claims (9)

Preparing a silkworm injected with an immunity inducing substance; And
And adding the silkworm to produce a feed composition. ≪ RTI ID = 0.0 > 21. < / RTI >
The method according to claim 1,
Wherein the feed composition further comprises an excipient and 1 to 50 parts by weight of the silkworm and 50 to 99 parts by weight of the excipient are added to 100 parts by weight of the total feed composition. Way.
The method of claim 2,
Wherein the excipient comprises silkworm-enriched silkworm-containing silkworm comprising at least one of horse chestnut, limestone, ground cornstarch, corn gluten feed, and soybean.
The method according to claim 1,
Wherein the silkworm is added to the feed composition in powder or liquid form.
The method according to claim 1,
Wherein the feed composition is in powder or pellet form. ≪ RTI ID = 0.0 > 21. < / RTI >
The method according to claim 1,
Wherein preparing the silkworm comprises:
And drying the silkworm by using a microwave.
The method according to claim 1,
Wherein the feed composition is feed of an animal of any one or more of chicken, duck, goose, pheasant, pig, cow, goat, dog, and cat.
The method according to claim 1,
Wherein the silkworm is added to the feed at a rate of 0.005 to 0.05% of the mass of the whole feed composition.
A feed composition prepared by the method for producing a feed composition containing silkworm peptide-enriched silkworm according to any one of claims 1 to 8.

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