WO2021135088A1 - Feed for improving fish immunity and preparation method therefor - Google Patents

Feed for improving fish immunity and preparation method therefor Download PDF

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Publication number
WO2021135088A1
WO2021135088A1 PCT/CN2020/096421 CN2020096421W WO2021135088A1 WO 2021135088 A1 WO2021135088 A1 WO 2021135088A1 CN 2020096421 W CN2020096421 W CN 2020096421W WO 2021135088 A1 WO2021135088 A1 WO 2021135088A1
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Prior art keywords
parts
portions
powder
feed
flour
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PCT/CN2020/096421
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French (fr)
Chinese (zh)
Inventor
韩健宝
杨秀华
王婷
张秀梅
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南京坤牧有机农业有限公司
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Publication of WO2021135088A1 publication Critical patent/WO2021135088A1/en

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/24—Animal feeding-stuffs from material of animal origin from blood
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/111—Aromatic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/195—Antibiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/26—Compounds containing phosphorus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Definitions

  • the invention relates to the field of feed technology, in particular to a feed for improving fish immunity and a preparation method thereof.
  • aquatic plants that use aquatic plants as the main food; silver carp and bighead that feed on plankton; omnivorous carp, crucian carp, etc.; other such as flowers, wheat ear fish, silver carp, barbarian fish , Monopterus albus, Moray eel, Loach, Loach, Catfish and common ferocious fishes such as black snakehead, mandarin fish, and eel; in addition, there are also mild carnivorous fish such as red scorpionfish, mongolian red scorpionfish and herring.
  • mild carnivorous fish such as red scorpionfish, mongolian red scorpionfish and herring.
  • the present invention provides a feed for improving fish immunity and a preparation method thereof.
  • a feed for improving fish immunity prepared from the following raw materials by weight:
  • the preparation method of the freeze-dried chlorella powder is as follows:
  • 1L of buffer solution contains 8-10mL of 1mol/L sodium hydroxide, 1-1.5mL of concentrated phosphoric acid, and the rest is normal saline.
  • the mixing volume ratio of the filtrate and the buffer in S1 is 1:1-1.5.
  • the preparation method of the above feed for improving fish immunity is as follows:
  • S1 Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
  • S3 Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
  • the temperature during the aging treatment is 140-150°C, and the steam pressure is 0.4-0.6 MPa.
  • Bean cake, silkworm pupa powder, distiller’s grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, soybean lecithin, calcium hydrogen phosphate, pine pollen, and defatted krill powder in the feed of the present invention can provide fish growth
  • the necessary nutrients have a positive effect on promoting fish growth and improving meat quality.
  • the ferulic acid in the feed can scavenge free radicals, promote the production of free radical scavenging enzymes, and increase glutathione transferase and quinone reduction.
  • Enzyme activity and inhibition of tyrosinase activity can regulate fish physiological functions, antibacterial and antiviral, chlorella freeze-dried powder is rich in biologically active substances, rich in anti-tumor active substances and sugars that can enhance immunity Lipids, peptides and glycoproteins, and its unique growth-promoting factor CGF can not only promote the growth of fish, improve production performance, but also increase the body’s immunity.
  • Cecropin antimicrobial peptide is one of the most potential antibiotic substitutes. Gram-positive bacteria and some gram-negative bacteria have strong lethality, with broad-spectrum, high efficiency, stability, rapid sterilization ability and immune system interaction, which can reduce fish infection rate and death
  • the feed of the present invention can significantly improve the growth performance and immune activity of yellow croaker.
  • the ferulic acid, chlorella freeze-dried powder, and cecropin antimicrobial peptide in the feed of the present invention have a synergistic effect with each other and can improve The weight gain rate of yellow croaker reduces the feed coefficient and improves immunity.
  • a feed for improving fish immunity prepared from the following raw materials by weight:
  • chlorella freeze-dried powder is as follows:
  • the preparation method of the above feed for improving fish immunity is as follows:
  • S1 Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
  • S3 Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
  • a feed for improving fish immunity prepared from the following raw materials by weight:
  • chlorella freeze-dried powder is as follows:
  • S2 Place the solution in a vacuum freeze dryer at minus 35°C for 20 hours at normal pressure, and then evacuate until the vacuum of the vacuum freeze dryer is -0.08MPa. At this time, start heating up at a rate of 4°C/min, and wait for the temperature to reach At minus 5 °C, keep it for 6 hours, then naturally warm to room temperature, after standing for 10 hours, return to normal pressure, suction filtration, and vacuum low temperature drying.
  • the preparation method of the above feed for improving fish immunity is as follows:
  • S1 Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
  • S3 Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
  • a feed for improving fish immunity prepared from the following raw materials by weight:
  • chlorella freeze-dried powder is as follows:
  • the preparation method of the above feed for improving fish immunity is as follows:
  • S1 Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
  • S3 Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
  • a feed for improving fish immunity prepared from the following raw materials by weight:
  • chlorella freeze-dried powder is as follows:
  • the preparation method of the above feed for improving fish immunity is as follows:
  • S1 Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
  • S3 Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
  • a feed for improving fish immunity prepared from the following raw materials by weight:
  • chlorella freeze-dried powder is as follows:
  • the preparation method of the above feed for improving fish immunity is as follows:
  • S1 Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
  • S3 Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
  • a feed for improving fish immunity prepared from the following raw materials by weight:
  • chlorella freeze-dried powder is as follows:
  • S2 Place the solution in a vacuum freeze dryer at minus 35°C for 20 hours at normal pressure, and then evacuate until the vacuum of the vacuum freeze dryer is -0.08MPa. At this time, start heating up at a rate of 4°C/min, and wait for the temperature to reach At minus 5 °C, keep it for 6 hours, then naturally warm to room temperature, after standing for 10 hours, return to normal pressure, suction filtration, and vacuum low temperature drying.
  • the preparation method of the above feed for improving fish immunity is as follows:
  • S1 Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
  • S3 Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
  • a feed for improving fish immunity prepared from the following raw materials by weight:
  • Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that it does not contain ferulic acid, and the rest are exactly the same.
  • a feed for improving fish immunity prepared from the following raw materials by weight:
  • Comparative Example 2 does not contain chlorella lyophilized powder, and the rest are exactly the same.
  • a feed for improving fish immunity prepared from the following raw materials by weight:
  • Comparative Example 3 does not contain cecropin antimicrobial peptide, and the rest are completely the same.
  • Example 1 The feeds prepared in Example 1 and Comparative Examples 1-3 were fed according to the general method. During the feeding period, the daily feeding management, disease prevention and other work were carried out according to the conventional management method, and the feeding time was 40 days.
  • the experimental results are shown in the following table:
  • Weight gain rate/% (tail weight at the end of the experiment-tail weight at the beginning of the experiment)/tail weight at the beginning of the experiment ⁇ 100;
  • Feed coefficient total feeding amount/total weight gain
  • the immunological indicators such as superoxide dismutase (SOD), lysozyme (LZM), alkaline phosphatase (AKP) and so on were measured, and the measurement and calculation methods were carried out using the kit instructions provided by Nanjing Jiancheng Institute of Bioengineering .
  • the feed of the present invention can significantly improve the growth performance and immune activity of yellow croaker.
  • the ferulic acid, chlorella freeze-dried powder, and cecropin antimicrobial peptide in the feed of the present invention have a synergistic effect with each other. , Can increase the weight gain rate of yellow croaker, reduce the feed coefficient and improve immunity.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Insects & Arthropods (AREA)
  • Birds (AREA)
  • Inorganic Chemistry (AREA)
  • Fodder In General (AREA)

Abstract

A feed for improving fish immunity and a preparation method therefor, relating to the technical field of feeds. The feed is prepared from the following raw materials in parts by weight: 50-60 parts of soybean cake, 10-15 parts of silkworm chrysalis powder, 1-10 parts of distiller's grains, 5-10 parts of barley flour, 20-30 parts of corn flour, 5-10 parts of bran, 5-10 parts of sweet potato flour, 5-10 parts of locust tree leaf powder, 1-5 parts of blood meal, 3-5 parts of soybean lecithin, 3-6 parts of calcium phosphate, 5-10 parts of pine pollen, 2-4 parts of ferulic acid, 5-10 parts of chlorella lyophilized powder, 2-4 parts of degreased krill powder, 1-2 parts of cecropin, and 0.1-1 parts of sodium propionate. The feed can improve the growth performance and immune activity of yellow croaker. It can be determined from comparative experimentations that the ferulic acid, the chlorella lyophilized powder and the cecropin in the feed have mutual synergistic effects, increasing the weight gain rate of yellow croaker, reducing the feed coefficient, and increasing the immunity.

Description

一种提高鱼类免疫力饲料及其制备方法Feed for improving fish immunity and preparation method thereof 技术领域Technical field
本发明涉及饲料技术领域，具体涉及一种提高鱼类免疫力饲料及其制备方法。The invention relates to the field of feed technology, in particular to a feed for improving fish immunity and a preparation method thereof.
背景技术Background technique
我国淡水鱼类约有800余种。其中，鲤科属种最多，有400余种，约占全部淡水鱼的二分之一；鲶科和鳅科的属种也不少，两科共有200余种，约占全部淡水鱼的四分之一。在我国的淡水鱼中，有些种类分布很广，几乎到处可见。如以水草为主要食料的草鱼、鳊鱼、三角鲂、赤眼鳟等；以浮游生物为食的鲢、鳙等；杂食性的鲤、鲫等；其他如花、麦穗鱼、银鲴、棒花鱼、黄鳝、白鳝、花鳅、泥鳅、鲶鱼以及常见凶猛鱼类乌鳢、鳜鱼、鳡等；此外还有性情温和的肉食性鱼类翘嘴红鲌、蒙古红鲌和青鱼等。There are about 800 species of freshwater fish in my country. Among them, the genus Cyprinidae has the most species, with more than 400 species, accounting for about one-half of all freshwater fish. There are also many genera and species of Siluriaceae and Loach. There are more than 200 species in the two families, accounting for about four of all freshwater fish. One part. In our country's freshwater fish, some species are widely distributed and can be seen almost everywhere. Such as grass carp, bream, triangle bream, red-eye trout, etc. that use aquatic plants as the main food; silver carp and bighead that feed on plankton; omnivorous carp, crucian carp, etc.; other such as flowers, wheat ear fish, silver carp, barbarian fish , Monopterus albus, Moray eel, Loach, Loach, Catfish and common ferocious fishes such as black snakehead, mandarin fish, and eel; in addition, there are also mild carnivorous fish such as red scorpionfish, mongolian red scorpionfish and herring.
随着淡水鱼养殖规模的逐渐扩大，高品质的鱼饲料也成为人们研究的重点，目前市场上使用的鱼饲料往往是营养成分相对单一、脂肪含量过高，大多还含有促生长剂、抗生素、其他化学合成类抗菌药物及合成防腐剂，他们配合饲料使用，对提高动物生产力水平、抗病能力及免疫力具有重要的作用。然而，目前市场上一些非营养性药物添加剂有其不足之处，如果长期使用，很容易使病原菌株产生抗药性，易导致药物残留量增加，会对饲养动物有一定的危害。研究开发无污染、无残留、绿色安全、不产生抗药性的饲料添加剂已经成为了研究的热点问题，也是未来饲料行业发展的趋势。With the gradual expansion of the scale of freshwater fish farming, high-quality fish feed has also become the focus of people’s research. The fish feed currently used in the market often has relatively single nutrients and high fat content, and most of them also contain growth promoters, antibiotics, Other chemically synthesized antibacterial drugs and synthetic preservatives, which are used in combination with feed, play an important role in improving animal productivity, disease resistance and immunity. However, some non-nutritive drug additives currently on the market have their shortcomings. If used for a long time, it is easy to make pathogenic strains resistant to drugs, which can easily lead to an increase in drug residues, which will cause certain harm to the raised animals. The research and development of feed additives that are pollution-free, residue-free, green and safe, and non-drug-resistant have become a hot topic in research and the development trend of the feed industry in the future.
发明内容Summary of the invention
发明目的：针对上述技术问题，本发明提供了一种提高鱼类免疫力饲料及其制备方法。Objective of the invention: In view of the above technical problems, the present invention provides a feed for improving fish immunity and a preparation method thereof.
为了达到上述发明目的，本发明所采用的技术方案如下：In order to achieve the above-mentioned purpose of the invention, the technical solutions adopted by the invention are as follows:
一种提高鱼类免疫力饲料，由以下重量份的原料制备而成：A feed for improving fish immunity, prepared from the following raw materials by weight:
豆饼50-60份、蚕蛹粉10-15份、酒糟1-10份、大麦粉5-10份、玉米粉20-30份、麸皮5-10份、红薯粉5-10份、槐树叶粉5-10份、血粉1-5份、大豆卵磷脂3-5份、磷酸氢钙3-6份、松花粉5-10份、阿魏酸2-4份、小球藻冻干粉5-10份、脱脂磷虾粉2-4份、天蚕素抗菌肽1-2份、丙酸钠0.1-1份。50-60 portions of bean cake, 10-15 portions of silkworm pupa powder, 1-10 portions of vinasse, 5-10 portions of barley flour, 20-30 portions of corn flour, 5-10 portions of bran, 5-10 portions of sweet potato flour, locust leaf powder 5-10 parts, blood meal 1-5 parts, soy lecithin 3-5 parts, dicalcium phosphate 3-6 parts, pine pollen 5-10 parts, ferulic acid 2-4 parts, chlorella freeze-dried powder 5- 10 parts, 2-4 parts of defatted krill powder, 1-2 parts of cecropin antimicrobial peptide, and 0.1-1 parts of sodium propionate.
进一步地，由以下重量份的原料制备而成：Further, it is prepared from the following parts by weight of raw materials:
豆饼55份、蚕蛹粉12份、酒糟4份、大麦粉8份、玉米粉25份、麸皮8份、红薯粉8份、槐树叶粉5份、血粉5份、大豆卵磷脂5份、磷酸氢钙3份、松花粉6份、阿魏酸4份、小球藻冻干粉5份、脱脂磷虾粉2份、天蚕素抗菌肽1.5份、丙酸钠0.2份。55 portions of bean cake, 12 portions of silkworm pupa powder, 4 portions of distillers grains, 8 portions of barley flour, 25 portions of corn flour, 8 portions of bran, 8 portions of sweet potato flour, 5 portions of locust leaf powder, 5 portions of blood meal, 5 portions of soy lecithin, phosphoric acid 3 parts of calcium hydrogen, 6 parts of pine pollen, 4 parts of ferulic acid, 5 parts of chlorella lyophilized powder, 2 parts of defatted krill powder, 1.5 parts of cecropin antimicrobial peptide, and 0.2 parts of sodium propionate.
进一步地，由以下重量份的原料制备而成：Further, it is prepared from the following parts by weight of raw materials:
豆饼50份、蚕蛹粉15份、酒糟2份、大麦粉10份、玉米粉20份、麸皮5份、红薯粉5份、槐树叶粉5份、血粉2份、大豆卵磷脂3份、磷酸氢钙3份、松花粉5份、阿魏酸2份、小球藻冻干粉10份、脱脂磷虾粉4份、天蚕素抗菌肽2份、丙酸钠0.5份。50 portions of bean cake, 15 portions of silkworm pupa powder, 2 portions of distillers grains, 10 portions of barley flour, 20 portions of corn flour, 5 portions of bran, 5 portions of sweet potato flour, 5 portions of locust leaf powder, 2 portions of blood meal, 3 portions of soy lecithin, phosphoric acid 3 parts of calcium hydrogen, 5 parts of pine pollen, 2 parts of ferulic acid, 10 parts of lyophilized chlorella powder, 4 parts of defatted krill powder, 2 parts of cecropin antimicrobial peptide, 0.5 parts of sodium propionate.
进一步地，所述小球藻冻干粉的制备方法如下：Further, the preparation method of the freeze-dried chlorella powder is as follows:
S1：将小球藻用清水洗净后阴干表面水分，放入榨汁机中榨汁，榨汁后滤布抽滤、微滤膜过滤后的滤液与缓冲液混合；S1: Wash the chlorella with clean water and dry the surface moisture in the shade, put it in a juicer to squeeze the juice, after squeezing the juice, filter the filter cloth, filter the filtrate with the microfiltration membrane and mix with the buffer;
S2：将溶液于零下35℃的真空冷冻干燥机中常压放置10-20h，抽真空至真空冷冻干燥机的真空度为-0.08MPa，此时开始升温，升温速度为2-4℃/min，待温度为零下5℃时，保温4-6h，再自然升温至室温，静置5-10h后，恢复常压，抽滤，真空低温干燥即可。S2: Place the solution in a vacuum freeze dryer at minus 35°C at normal pressure for 10-20 hours, and then vacuum until the vacuum of the vacuum freeze dryer is -0.08MPa. At this time, the temperature starts to rise, and the temperature rise rate is 2-4°C/min. When the temperature is minus 5℃, keep it for 4-6h, then naturally warm up to room temperature, after standing for 5-10h, return to normal pressure, suction filtration, and vacuum low-temperature drying.
进一步地，1L缓冲液中含有1mol/L的氢氧化钠8-10mL，浓磷酸1-1.5mL，其余为生理盐水。Further, 1L of buffer solution contains 8-10mL of 1mol/L sodium hydroxide, 1-1.5mL of concentrated phosphoric acid, and the rest is normal saline.
进一步地，S1中滤液与缓冲液混合的体积比为1:1-1.5。Further, the mixing volume ratio of the filtrate and the buffer in S1 is 1:1-1.5.
上述提高鱼类免疫力饲料的制备方法具体如下：The preparation method of the above feed for improving fish immunity is as follows:
S1：将豆饼、蚕蛹粉、酒糟、大麦粉、玉米粉、麸皮、红薯粉、槐树叶粉、血粉、磷酸氢钙、松花粉分别烘干至含水量10％以下后加入到混料机中混合均匀；S1: Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
S2：再将混合物料送入调制器中，熟化处理5-10min；S2: Send the mixed material to the modulator and mature for 5-10min;
S3：将熟化后的物料送入膨化机中，再将大豆卵磷脂加入，进行膨化处理，膨化后的物料冷却后烘干至含水量5％以下，利用粉碎机粉碎并过80目筛网，加入到混料机中再将阿魏酸、小球藻冻干粉、脱脂磷虾粉、天蚕素抗菌肽、丙酸钠加入混合均匀后造粒即可。S3: Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
进一步地，熟化处理时的温度为140-150℃，蒸汽压力为0.4-0.6MPa。Further, the temperature during the aging treatment is 140-150°C, and the steam pressure is 0.4-0.6 MPa.
本发明的有益效果：The beneficial effects of the present invention:
本发明饲料中豆饼、蚕蛹粉、酒糟、大麦粉、玉米粉、麸皮、红薯粉、槐树叶粉、血粉、大豆卵磷脂、磷酸氢钙、松花粉、脱脂磷虾粉可以提供鱼类生长所必须的各项营养成分，对于促进鱼类生长，改善肉质有积极作用，饲料中的阿魏酸能清除自由基，促进清除自由基的酶的产生，增加谷胱甘肽转硫酶和醌还原酶的活性，并抑制酪氨酸酶活性，可以调节鱼类生理机能，抗菌抗病毒，小球藻冻干粉含有丰富的生物活性物质，富含能够抗肿瘤活性物质以及能增强免疫力的糖脂、多肽和糖蛋白，而且其特有的促生长因子CGF不仅能促进鱼类的生长，提高生产性能，还能增加机体免疫力，天蚕素抗菌肽作为最具潜力的抗生素替代品之一，对革兰氏阳性菌、部分革兰氏阴性菌具有很强的杀伤力，具有广谱性、高效性、稳定性、快速杀菌能力和免疫系统的相互作用等特点，可以降低鱼类染病率和死亡率，本发明饲料可显著提高黄鱼的生长性能和免疫活性，通过对比试验可知，本发明饲料中的阿魏酸、小球藻冻干粉、天蚕素抗菌肽有相互间的协同作用，可以提高黄鱼的增重率，降低饲料系数，提高免疫力。Bean cake, silkworm pupa powder, distiller’s grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, soybean lecithin, calcium hydrogen phosphate, pine pollen, and defatted krill powder in the feed of the present invention can provide fish growth The necessary nutrients have a positive effect on promoting fish growth and improving meat quality. The ferulic acid in the feed can scavenge free radicals, promote the production of free radical scavenging enzymes, and increase glutathione transferase and quinone reduction. Enzyme activity and inhibition of tyrosinase activity, can regulate fish physiological functions, antibacterial and antiviral, chlorella freeze-dried powder is rich in biologically active substances, rich in anti-tumor active substances and sugars that can enhance immunity Lipids, peptides and glycoproteins, and its unique growth-promoting factor CGF can not only promote the growth of fish, improve production performance, but also increase the body’s immunity. Cecropin antimicrobial peptide is one of the most potential antibiotic substitutes. Gram-positive bacteria and some gram-negative bacteria have strong lethality, with broad-spectrum, high efficiency, stability, rapid sterilization ability and immune system interaction, which can reduce fish infection rate and death The feed of the present invention can significantly improve the growth performance and immune activity of yellow croaker. Through comparative experiments, it can be known that the ferulic acid, chlorella freeze-dried powder, and cecropin antimicrobial peptide in the feed of the present invention have a synergistic effect with each other and can improve The weight gain rate of yellow croaker reduces the feed coefficient and improves immunity.
具体实施方式Detailed ways
实施例中未注明具体条件者，按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者，均为可以通过市售购买获得的常规产品。If no specific conditions are indicated in the examples, it shall be carried out in accordance with the conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used without the manufacturer's indication are all conventional products that can be purchased on the market.
实施例1：Example 1:
一种提高鱼类免疫力饲料，由以下重量份的原料制备而成：A feed for improving fish immunity, prepared from the following raw materials by weight:
豆饼55份、蚕蛹粉12份、酒糟4份、大麦粉8份、玉米粉25份、麸皮8份、红薯粉8份、槐树叶粉5份、血粉5份、大豆卵磷脂5份、磷酸氢钙3份、松花粉6份、阿魏酸4份、小球藻冻干粉5份、脱脂磷虾粉2份、天蚕素抗菌肽1.5份、丙酸钠0.2份。55 portions of bean cake, 12 portions of silkworm pupa powder, 4 portions of distillers grains, 8 portions of barley flour, 25 portions of corn flour, 8 portions of bran, 8 portions of sweet potato flour, 5 portions of locust leaf powder, 5 portions of blood meal, 5 portions of soy lecithin, phosphoric acid 3 parts of calcium hydrogen, 6 parts of pine pollen, 4 parts of ferulic acid, 5 parts of chlorella lyophilized powder, 2 parts of defatted krill powder, 1.5 parts of cecropin antimicrobial peptide, and 0.2 parts of sodium propionate.
其中，小球藻冻干粉的制备方法如下：Among them, the preparation method of chlorella freeze-dried powder is as follows:
S1：将小球藻用清水洗净后阴干表面水分，放入榨汁机中榨汁，榨汁后滤布抽滤、微滤膜过滤后的滤液与缓冲液按体积比1:1混合，1L缓冲液中含有1mol/L的氢氧化钠10mL，浓磷酸1.5mL，其余为生理盐水；S1: Wash the chlorella with clean water and dry the surface moisture in the shade, put it in a juicer to squeeze the juice. After squeezing the juice, filter the filter cloth, filter the filtrate with the microfiltration membrane and mix the buffer at a volume ratio of 1:1. 1L buffer contains 10mL of 1mol/L sodium hydroxide, 1.5mL of concentrated phosphoric acid, and the rest is normal saline;
S2：将溶液于零下35℃的真空冷冻干燥机中常压放置15h，抽真空至真空冷冻干燥机的真空度为-0.08MPa，此时开始升温，升温速度为2℃/min，待温度为零下5℃时，保温6h，再自然升温至室温，静置10h后，恢复常压，抽滤，真空低温干燥即可。S2: Place the solution in a vacuum freeze dryer at minus 35°C for 15 hours at normal pressure, and then evacuate until the vacuum degree of the vacuum freeze dryer is -0.08MPa. At this time, start heating up at a rate of 2°C/min, and wait until the temperature reaches At minus 5 ℃, keep it for 6 hours, then naturally warm to room temperature, after standing for 10 hours, return to normal pressure, suction filtration, and vacuum low temperature drying.
上述提高鱼类免疫力饲料的制备方法具体如下：The preparation method of the above feed for improving fish immunity is as follows:
S1：将豆饼、蚕蛹粉、酒糟、大麦粉、玉米粉、麸皮、红薯粉、槐树叶粉、血粉、磷酸氢钙、松花粉分别烘干至含水量10％以下后加入到混料机中混合均匀；S1: Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
S2：再将混合物料送入调制器中，熟化处理5min，熟化处理时的温度为140℃，蒸汽压力为0.5MPa；S2: Send the mixed material into the modulator for 5 minutes of maturation treatment, the temperature during maturation treatment is 140°C, and the steam pressure is 0.5MPa;
S3：将熟化后的物料送入膨化机中，再将大豆卵磷脂加入，进行膨化处理，膨化后的物料冷却后烘干至含水量5％以下，利用粉碎机粉碎并过80目筛网，加入到混料机中再将阿魏酸、小球藻冻干粉、脱脂磷虾粉、天蚕素抗菌肽、丙酸钠加入混合均匀后造粒即可。S3: Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
实施例2：Example 2:
一种提高鱼类免疫力饲料，由以下重量份的原料制备而成：A feed for improving fish immunity, prepared from the following raw materials by weight:
豆饼50份、蚕蛹粉15份、酒糟2份、大麦粉10份、玉米粉20份、麸皮5份、红薯粉5份、槐树叶粉5份、血粉2份、大豆卵磷脂3份、磷酸氢钙3份、松花粉5份、阿魏酸2份、小球藻冻干粉10份、脱脂磷虾粉4份、天蚕素抗菌肽2份、丙酸钠0.5份。50 portions of bean cake, 15 portions of silkworm pupa powder, 2 portions of distillers grains, 10 portions of barley flour, 20 portions of corn flour, 5 portions of bran, 5 portions of sweet potato flour, 5 portions of locust leaf powder, 2 portions of blood meal, 3 portions of soy lecithin, phosphoric acid 3 parts of calcium hydrogen, 5 parts of pine pollen, 2 parts of ferulic acid, 10 parts of lyophilized chlorella powder, 4 parts of defatted krill powder, 2 parts of cecropin antimicrobial peptide, 0.5 parts of sodium propionate.
其中，小球藻冻干粉的制备方法如下：Among them, the preparation method of chlorella freeze-dried powder is as follows:
S1：将小球藻用清水洗净后阴干表面水分，放入榨汁机中榨汁，榨汁后滤布抽滤、微滤膜过滤后的滤液与缓冲液按体积比1:1混合，1L缓冲液中含有1mol/L的氢氧化钠10mL，浓磷酸1.5mL，其余为生理盐水；S1: Wash the chlorella with clean water and dry the surface moisture in the shade, put it in a juicer to squeeze the juice. After squeezing the juice, filter the filter cloth, filter the filtrate with the microfiltration membrane and mix the buffer at a volume ratio of 1:1. 1L buffer contains 10mL of 1mol/L sodium hydroxide, 1.5mL of concentrated phosphoric acid, and the rest is normal saline;
S2：将溶液于零下35℃的真空冷冻干燥机中常压放置20h，抽真空至真空冷冻干燥机的真空度为-0.08MPa，此时开始升温，升温速度为4℃/min，待温度为零下5℃时，保温6h，再自然升温至室温，静置10h后，恢复常压，抽滤，真空低温干燥即可。S2: Place the solution in a vacuum freeze dryer at minus 35°C for 20 hours at normal pressure, and then evacuate until the vacuum of the vacuum freeze dryer is -0.08MPa. At this time, start heating up at a rate of 4°C/min, and wait for the temperature to reach At minus 5 ℃, keep it for 6 hours, then naturally warm to room temperature, after standing for 10 hours, return to normal pressure, suction filtration, and vacuum low temperature drying.
上述提高鱼类免疫力饲料的制备方法具体如下：The preparation method of the above feed for improving fish immunity is as follows:
S1：将豆饼、蚕蛹粉、酒糟、大麦粉、玉米粉、麸皮、红薯粉、槐树叶粉、血粉、磷酸氢钙、松花粉分别烘干至含水量10％以下后加入到混料机中混合均匀；S1: Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
S2：再将混合物料送入调制器中，熟化处理5min，熟化处理时的温度为140℃，蒸汽压力为0.5MPa；S2: Send the mixed material into the modulator for 5 minutes of maturation treatment, the temperature during maturation treatment is 140°C, and the steam pressure is 0.5MPa;
S3：将熟化后的物料送入膨化机中，再将大豆卵磷脂加入，进行膨化处理，膨化后的物料冷却后烘干至含水量5％以下，利用粉碎机粉碎并过80目筛网，加入到混料机中再将阿魏酸、小球藻冻干粉、脱脂磷虾粉、天蚕素抗菌肽、丙酸钠加入混合均匀后造粒即可。S3: Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
实施例3：Example 3:
一种提高鱼类免疫力饲料，由以下重量份的原料制备而成：A feed for improving fish immunity, prepared from the following raw materials by weight:
豆饼55份、蚕蛹粉15份、酒糟5份、大麦粉8份、玉米粉20份、麸皮8份、红薯粉10份、槐树叶粉5份、血粉2份、大豆卵磷脂3份、磷酸氢钙3份、松花粉6份、阿魏酸2份、小球藻冻干粉5份、脱脂磷虾粉2份、天蚕素抗菌肽2份、丙酸钠0.5份。55 servings of bean cake, 15 servings of silkworm pupa powder, 5 servings of distillers grains, 8 servings of barley flour, 20 servings of corn flour, 8 servings of bran, 10 servings of sweet potato powder, 5 servings of locust leaf powder, 2 servings of blood meal, 3 servings of soy lecithin, phosphoric acid 3 parts of calcium hydrogen, 6 parts of pine pollen, 2 parts of ferulic acid, 5 parts of chlorella lyophilized powder, 2 parts of defatted krill powder, 2 parts of cecropin antimicrobial peptide, 0.5 parts of sodium propionate.
其中，小球藻冻干粉的制备方法如下：Among them, the preparation method of chlorella freeze-dried powder is as follows:
S1：将小球藻用清水洗净后阴干表面水分，放入榨汁机中榨汁，榨汁后滤布抽滤、微滤膜过滤后的滤液与缓冲液按体积比1:1.5混合，1L缓冲液中含有1mol/L的氢氧化钠10mL，浓磷酸1.5mL，其余为生理盐水；S1: Wash the chlorella with clean water and dry the surface water in the shade, put it in a juicer to squeeze the juice, after squeezing the juice, filter the filter cloth, filter the filtrate with the microfiltration membrane and mix with the buffer in a volume ratio of 1:1.5. 1L buffer contains 10mL of 1mol/L sodium hydroxide, 1.5mL of concentrated phosphoric acid, and the rest is normal saline;
S2：将溶液于零下35℃的真空冷冻干燥机中常压放置10h，抽真空至真空冷冻干燥机的真空度为-0.08MPa，此时开始升温，升温速度为3℃/min，待温度为零下5℃时，保温6h，再自然升温至室温，静置10h后，恢复常压，抽滤，真空低温干燥即可。S2: Place the solution in a vacuum freeze dryer at minus 35°C for 10 hours at normal pressure, and then evacuate until the vacuum degree of the vacuum freeze dryer is -0.08MPa. At this time, start heating up at a rate of 3°C/min, and wait until the temperature reaches At minus 5 ℃, keep it for 6 hours, then naturally warm to room temperature, after standing for 10 hours, return to normal pressure, suction filtration, and vacuum low temperature drying.
上述提高鱼类免疫力饲料的制备方法具体如下：The preparation method of the above feed for improving fish immunity is as follows:
S1：将豆饼、蚕蛹粉、酒糟、大麦粉、玉米粉、麸皮、红薯粉、槐树叶粉、血粉、磷酸氢钙、松花粉分别烘干至含水量10％以下后加入到混料机中混合均匀；S1: Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
S2：再将混合物料送入调制器中，熟化处理8min，熟化处理时的温度为145℃，蒸汽压力为0.6MPa；S2: Send the mixed material into the modulator for 8 minutes of maturation treatment, the temperature during maturation treatment is 145℃, and the steam pressure is 0.6MPa;
S3：将熟化后的物料送入膨化机中，再将大豆卵磷脂加入，进行膨化处理，膨化后的物料冷却后烘干至含水量5％以下，利用粉碎机粉碎并过80目筛网，加入到混料机中再将阿魏酸、小球藻冻干粉、脱脂磷虾粉、天蚕素抗菌肽、丙酸钠加入混合均匀后造粒即可。S3: Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
实施例4：Example 4:
一种提高鱼类免疫力饲料，由以下重量份的原料制备而成：A feed for improving fish immunity, prepared from the following raw materials by weight:
豆饼60份、蚕蛹粉12份、酒糟2份、大麦粉5份、玉米粉25份、麸皮5份、红薯粉10份、槐树叶粉10份、血粉1份、大豆卵磷脂4份、磷酸氢钙3份、松花粉10份、阿魏酸4份、小球藻冻干粉5份、脱脂磷虾粉3份、天蚕素抗菌肽2份、丙酸钠0.2份。60 portions of bean cake, 12 portions of silkworm pupa powder, 2 portions of distillers grains, 5 portions of barley flour, 25 portions of corn flour, 5 portions of bran, 10 portions of sweet potato flour, 10 portions of locust leaf powder, 1 portion of blood meal, 4 portions of soy lecithin, phosphoric acid 3 parts of calcium hydrogen, 10 parts of pine pollen, 4 parts of ferulic acid, 5 parts of chlorella lyophilized powder, 3 parts of defatted krill powder, 2 parts of cecropin antimicrobial peptide, and 0.2 part of sodium propionate.
其中，小球藻冻干粉的制备方法如下：Among them, the preparation method of chlorella freeze-dried powder is as follows:
S1：将小球藻用清水洗净后阴干表面水分，放入榨汁机中榨汁，榨汁后滤布抽滤、微滤膜过滤后的滤液与缓冲液按体积比1:1混合，1L缓冲液中含有1mol/L的氢氧化钠10mL，浓磷酸1.5mL，其余为生理盐水；S1: Wash the chlorella with clean water and dry the surface moisture in the shade, put it in a juicer to squeeze the juice. After squeezing the juice, filter the filter cloth, filter the filtrate with the microfiltration membrane and mix the buffer at a volume ratio of 1:1. 1L buffer contains 10mL of 1mol/L sodium hydroxide, 1.5mL of concentrated phosphoric acid, and the rest is normal saline;
S2：将溶液于零下35℃的真空冷冻干燥机中常压放置20h，抽真空至真空冷冻干燥机的真空度为-0.08MPa，此时开始升温，升温速度为2℃/min，待温度为零下5℃时，保温6h，再自然升温至室温，静置10h后，恢复常压，抽滤，真空低温干燥即可。S2: Place the solution in a vacuum freeze dryer at minus 35°C for 20 hours at normal pressure, and then evacuate until the vacuum of the vacuum freeze dryer is -0.08MPa. At this time, start to heat up at a rate of 2°C/min, and wait until the temperature reaches At minus 5 ℃, keep it for 6 hours, then naturally warm to room temperature, after standing for 10 hours, return to normal pressure, suction filtration, and vacuum low temperature drying.
上述提高鱼类免疫力饲料的制备方法具体如下：The preparation method of the above feed for improving fish immunity is as follows:
S1：将豆饼、蚕蛹粉、酒糟、大麦粉、玉米粉、麸皮、红薯粉、槐树叶粉、血粉、磷酸氢钙、松花粉分别烘干至含水量10％以下后加入到混料机中混合均匀；S1: Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
S2：再将混合物料送入调制器中，熟化处理5min，熟化处理时的温度为140℃，蒸汽压力为0.5MPa；S2: Send the mixed material into the modulator for 5 minutes of maturation treatment, the temperature during maturation treatment is 140°C, and the steam pressure is 0.5MPa;
S3：将熟化后的物料送入膨化机中，再将大豆卵磷脂加入，进行膨化处理，膨化后的物料冷却后烘干至含水量5％以下，利用粉碎机粉碎并过80目筛网，加入到混料机中再将阿魏酸、小球藻冻干粉、脱脂磷虾粉、天蚕素抗菌肽、丙酸钠加入混合均匀后造粒即可。S3: Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
实施例5：Example 5:
一种提高鱼类免疫力饲料，由以下重量份的原料制备而成：A feed for improving fish immunity, prepared from the following raw materials by weight:
豆饼50份、蚕蛹粉10份、酒糟1份、大麦粉5份、玉米粉20份、麸皮5份、红薯粉5份、槐树叶粉5份、血粉1份、大豆卵磷脂3份、磷酸氢钙3份、松花粉5份、阿魏酸2份、小球藻冻干粉5份、 脱脂磷虾粉2份、天蚕素抗菌肽1份、丙酸钠0.1份。50 portions of bean cake, 10 portions of silkworm pupa powder, 1 portion of distillers grains, 5 portions of barley flour, 20 portions of corn flour, 5 portions of bran, 5 portions of sweet potato flour, 5 portions of locust leaf powder, 1 portion of blood meal, 3 portions of soy lecithin, phosphoric acid 3 parts of calcium hydrogen, 5 parts of pine pollen, 2 parts of ferulic acid, 5 parts of chlorella lyophilized powder, 2 parts of defatted krill powder, 1 part of cecropin antimicrobial peptide, and 0.1 part of sodium propionate.
其中，小球藻冻干粉的制备方法如下：Among them, the preparation method of chlorella freeze-dried powder is as follows:
S1：将小球藻用清水洗净后阴干表面水分，放入榨汁机中榨汁，榨汁后滤布抽滤、微滤膜过滤后的滤液与缓冲液按体积比1:1混合，1L缓冲液中含有1mol/L的氢氧化钠8mL，浓磷酸1mL，其余为生理盐水；S1: Wash the chlorella with clean water and dry the surface moisture in the shade, put it in a juicer to squeeze the juice. After squeezing the juice, filter the filter cloth, filter the filtrate with the microfiltration membrane and mix the buffer at a volume ratio of 1:1. 1L buffer contains 8mL of 1mol/L sodium hydroxide, 1mL of concentrated phosphoric acid, and the rest is normal saline;
S2：将溶液于零下35℃的真空冷冻干燥机中常压放置10h，抽真空至真空冷冻干燥机的真空度为-0.08MPa，此时开始升温，升温速度为2℃/min，待温度为零下5℃时，保温4h，再自然升温至室温，静置5h后，恢复常压，抽滤，真空低温干燥即可。S2: Place the solution in a vacuum freeze dryer at minus 35°C under normal pressure for 10 hours, vacuum until the vacuum degree of the vacuum freeze dryer is -0.08MPa, then start to heat up at a rate of 2°C/min, and wait for the temperature to reach At minus 5°C, keep it for 4 hours, then naturally warm to room temperature. After standing for 5 hours, return to normal pressure, filter by suction, and dry in vacuum at low temperature.
上述提高鱼类免疫力饲料的制备方法具体如下：The preparation method of the above feed for improving fish immunity is as follows:
S1：将豆饼、蚕蛹粉、酒糟、大麦粉、玉米粉、麸皮、红薯粉、槐树叶粉、血粉、磷酸氢钙、松花粉分别烘干至含水量10％以下后加入到混料机中混合均匀；S1: Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
S2：再将混合物料送入调制器中，熟化处理5min，熟化处理时的温度为140℃，蒸汽压力为0.4MPa；S2: Send the mixed material into the modulator for 5 minutes of maturation treatment, the temperature during maturation treatment is 140°C, and the steam pressure is 0.4MPa;
S3：将熟化后的物料送入膨化机中，再将大豆卵磷脂加入，进行膨化处理，膨化后的物料冷却后烘干至含水量5％以下，利用粉碎机粉碎并过80目筛网，加入到混料机中再将阿魏酸、小球藻冻干粉、脱脂磷虾粉、天蚕素抗菌肽、丙酸钠加入混合均匀后造粒即可。S3: Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
实施例6：Example 6:
一种提高鱼类免疫力饲料，由以下重量份的原料制备而成：A feed for improving fish immunity, prepared from the following raw materials by weight:
豆饼60份、蚕蛹粉15份、酒糟10份、大麦粉10份、玉米粉30份、麸皮10份、红薯粉10份、槐树叶粉10份、血粉5份、大豆卵磷脂5份、磷酸氢钙6份、松花粉10份、阿魏酸4份、小球藻冻干粉10份、脱脂磷虾粉4份、天蚕素抗菌肽2份、丙酸钠1份。60 portions of bean cake, 15 portions of silkworm pupa powder, 10 portions of distillers grains, 10 portions of barley flour, 30 portions of corn flour, 10 portions of bran, 10 portions of sweet potato flour, 10 portions of locust leaf powder, 5 portions of blood meal, 5 portions of soy lecithin, phosphoric acid 6 parts of calcium hydrogen, 10 parts of pine pollen, 4 parts of ferulic acid, 10 parts of chlorella lyophilized powder, 4 parts of defatted krill powder, 2 parts of cecropin antimicrobial peptide, and 1 part of sodium propionate.
其中，小球藻冻干粉的制备方法如下：Among them, the preparation method of chlorella freeze-dried powder is as follows:
S1：将小球藻用清水洗净后阴干表面水分，放入榨汁机中榨汁，榨汁后滤布抽滤、微滤膜过滤后的滤液与缓冲液按体积比1:1.5混合，1L缓冲液中含有1mol/L的氢氧化钠10mL，浓磷酸1.5mL，其余为生理盐水；S1: Wash the chlorella with clean water and dry the surface water in the shade, put it in a juicer to squeeze the juice, after squeezing the juice, filter the filter cloth, filter the filtrate with the microfiltration membrane and mix with the buffer in a volume ratio of 1:1.5. 1L buffer contains 10mL of 1mol/L sodium hydroxide, 1.5mL of concentrated phosphoric acid, and the rest is normal saline;
S2：将溶液于零下35℃的真空冷冻干燥机中常压放置20h，抽真空至真空冷冻干燥机的真空度为-0.08MPa，此时开始升温，升温速度为4℃/min，待温度为零下5℃时，保温6h，再自然升温至室温，静置10h后，恢复常压，抽滤，真空低温干燥即可。S2: Place the solution in a vacuum freeze dryer at minus 35°C for 20 hours at normal pressure, and then evacuate until the vacuum of the vacuum freeze dryer is -0.08MPa. At this time, start heating up at a rate of 4°C/min, and wait for the temperature to reach At minus 5 ℃, keep it for 6 hours, then naturally warm to room temperature, after standing for 10 hours, return to normal pressure, suction filtration, and vacuum low temperature drying.
上述提高鱼类免疫力饲料的制备方法具体如下：The preparation method of the above feed for improving fish immunity is as follows:
S1：将豆饼、蚕蛹粉、酒糟、大麦粉、玉米粉、麸皮、红薯粉、槐树叶粉、血粉、磷酸氢钙、松花粉分别烘干至含水量10％以下后加入到混料机中混合均匀；S1: Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
S2：再将混合物料送入调制器中，熟化处理10min，熟化处理时的温度为150℃，蒸汽压力为0.6MPa；S2: Send the mixed material to the modulator, aging treatment for 10 minutes, the temperature during aging treatment is 150℃, and the steam pressure is 0.6MPa;
S3：将熟化后的物料送入膨化机中，再将大豆卵磷脂加入，进行膨化处理，膨化后的物料冷却后烘干至含水量5％以下，利用粉碎机粉碎并过80目筛网，加入到混料机中再将阿魏酸、小球藻冻干粉、脱脂磷虾粉、天蚕素抗菌肽、丙酸钠加入混合均匀后造粒即可。S3: Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
对比例1：Comparative example 1:
一种提高鱼类免疫力饲料，由以下重量份的原料制备而成：A feed for improving fish immunity, prepared from the following raw materials by weight:
豆饼55份、蚕蛹粉12份、酒糟4份、大麦粉8份、玉米粉25份、麸皮8份、红薯粉8份、槐树叶 粉5份、血粉5份、大豆卵磷脂5份、磷酸氢钙3份、松花粉6份、小球藻冻干粉5份、脱脂磷虾粉2份、天蚕素抗菌肽1.5份、丙酸钠0.2份。55 portions of bean cake, 12 portions of silkworm pupa powder, 4 portions of distillers grains, 8 portions of barley flour, 25 portions of corn flour, 8 portions of bran, 8 portions of sweet potato flour, 5 portions of locust leaf powder, 5 portions of blood meal, 5 portions of soy lecithin, phosphoric acid 3 parts of calcium hydrogen, 6 parts of pine pollen, 5 parts of chlorella lyophilized powder, 2 parts of defatted krill powder, 1.5 parts of cecropin antimicrobial peptide, and 0.2 parts of sodium propionate.
对比例1与实施例1的区别在于不含阿魏酸，其余完全相同。The difference between Comparative Example 1 and Example 1 is that it does not contain ferulic acid, and the rest are exactly the same.
对比例2：Comparative example 2:
一种提高鱼类免疫力饲料，由以下重量份的原料制备而成：A feed for improving fish immunity, prepared from the following raw materials by weight:
豆饼55份、蚕蛹粉12份、酒糟4份、大麦粉8份、玉米粉25份、麸皮8份、红薯粉8份、槐树叶粉5份、血粉5份、大豆卵磷脂5份、磷酸氢钙3份、松花粉6份、阿魏酸4份、脱脂磷虾粉2份、天蚕素抗菌肽1.5份、丙酸钠0.2份。55 portions of bean cake, 12 portions of silkworm pupa powder, 4 portions of distillers grains, 8 portions of barley flour, 25 portions of corn flour, 8 portions of bran, 8 portions of sweet potato flour, 5 portions of locust leaf powder, 5 portions of blood meal, 5 portions of soy lecithin, phosphoric acid 3 parts of calcium hydrogen, 6 parts of pine pollen, 4 parts of ferulic acid, 2 parts of defatted krill powder, 1.5 parts of cecropin antimicrobial peptide, and 0.2 parts of sodium propionate.
对比例2与实施例1的区别在于不含小球藻冻干粉，其余完全相同。The difference between Comparative Example 2 and Example 1 is that it does not contain chlorella lyophilized powder, and the rest are exactly the same.
对比例3：Comparative example 3:
一种提高鱼类免疫力饲料，由以下重量份的原料制备而成：A feed for improving fish immunity, prepared from the following raw materials by weight:
豆饼55份、蚕蛹粉12份、酒糟4份、大麦粉8份、玉米粉25份、麸皮8份、红薯粉8份、槐树叶粉5份、血粉5份、大豆卵磷脂5份、磷酸氢钙3份、松花粉6份、阿魏酸4份、小球藻冻干粉5份、脱脂磷虾粉2份、天蚕素抗菌肽1.5份、丙酸钠0.2份。55 portions of bean cake, 12 portions of silkworm pupa powder, 4 portions of distillers grains, 8 portions of barley flour, 25 portions of corn flour, 8 portions of bran, 8 portions of sweet potato flour, 5 portions of locust leaf powder, 5 portions of blood meal, 5 portions of soy lecithin, phosphoric acid 3 parts of calcium hydrogen, 6 parts of pine pollen, 4 parts of ferulic acid, 5 parts of chlorella lyophilized powder, 2 parts of defatted krill powder, 1.5 parts of cecropin antimicrobial peptide, and 0.2 parts of sodium propionate.
对比例3与实施例1的区别在于不含天蚕素抗菌肽，其余完全相同。The difference between Comparative Example 3 and Example 1 is that it does not contain cecropin antimicrobial peptide, and the rest are completely the same.
设置实验组和对比组1-3，每组放养黄鱼幼鱼100尾，体长(15.1±0.27)cm，体重(69.42±8.95)g，其中，实验组和对比组1-3分别饲喂实施例1和对比例1-3制备的饲料，按一般方式饲喂，饲喂期间日常的饲养管理、病害防治等工作按常规管理方法进行，饲喂时间40d，实验结果如下表所示：Set up the experimental group and the control group 1-3, each group stocked 100 yellow croaker juveniles, body length (15.1±0.27) cm, body weight (69.42±8.95) g, of which the experimental group and the control group 1-3 were fed separately The feeds prepared in Example 1 and Comparative Examples 1-3 were fed according to the general method. During the feeding period, the daily feeding management, disease prevention and other work were carried out according to the conventional management method, and the feeding time was 40 days. The experimental results are shown in the following table:
增重率/％＝(试验结束尾重－试验开始尾重)/试验开始尾重×100；Weight gain rate/%=(tail weight at the end of the experiment-tail weight at the beginning of the experiment)/tail weight at the beginning of the experiment×100;
日增重＝尾相对增重/试验天数；Daily gain = relative tail gain/test days;
饲料系数＝总投饵量/总增重；Feed coefficient=total feeding amount/total weight gain;
试验结束时，测定超氧化物歧化酶(SOD)、溶菌酶(LZM)、碱性磷酸酶(AKP)等免疫学指标，测定与计算方法都采用南京建成生物工程研究所提供的试剂盒说明书进行。At the end of the experiment, the immunological indicators such as superoxide dismutase (SOD), lysozyme (LZM), alkaline phosphatase (AKP) and so on were measured, and the measurement and calculation methods were carried out using the kit instructions provided by Nanjing Jiancheng Institute of Bioengineering .
Figure PCTCN2020096421-appb-000001
Figure PCTCN2020096421-appb-000001
由上表可知，本发明饲料可显著提高黄鱼的生长性能和免疫活性，通过对比试验可知，本发明饲料中的阿魏酸、小球藻冻干粉、天蚕素抗菌肽有相互间的协同作用，可以提高黄鱼的增重率，降低饲料系数，提高免疫力。It can be seen from the above table that the feed of the present invention can significantly improve the growth performance and immune activity of yellow croaker. Through comparative experiments, it can be known that the ferulic acid, chlorella freeze-dried powder, and cecropin antimicrobial peptide in the feed of the present invention have a synergistic effect with each other. , Can increase the weight gain rate of yellow croaker, reduce the feed coefficient and improve immunity.
需要说明的是，在本文中，诸如第一和第二等之类的关系术语仅仅用来将一个实体或者操作与另一个实体或操作区分开来，而不一定要求或者暗示这些实体或操作之间存在任何这种实际的关系或者顺序。而且，术语“包括”、“包含”或者其任何其他变体意在涵盖非排他性的包含，从而使得包括一系列要素的过程、方法、物品或者设备不仅包括那些要素，而且还包括没有明确列出的其他要素，或者是还包括为这种过程、方法、物品或者设备所固有的要素。在没有更多限制的情况下，由语句“包括一个……”限定的要素，并不排除在包括所述要素的过程、方法、物品或者设备中还存在另外的相同要素。It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply one of these entities or operations. There is any such actual relationship or order between. Moreover, the terms "include", "include" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes those that are not explicitly listed Other elements of, or also include elements inherent to this process, method, article or equipment. If there are no more restrictions, the element defined by the sentence "including a..." does not exclude the existence of other identical elements in the process, method, article, or equipment that includes the element.
以上实施例仅用以说明本发明的技术方案，而非对其限制；尽管参照前述实施例对本发明进行了详细的说明，本领域的普通技术人员应当理解：其依然可以对前述各实施例所记载的技术方案进行修改，或者对其中部分技术特征进行等同替换；而这些修改或者替换，并不使相应技术方案的本质脱离本发明各实施例技术方案的精神和范围。The above embodiments are only used to illustrate the technical solutions of the present invention, not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, a person of ordinary skill in the art should understand that: The recorded technical solutions are modified, or some of the technical features are equivalently replaced; these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims (8)

  1. 一种提高鱼类免疫力饲料，其特征在于，由以下重量份的原料制备而成：A feed for improving fish immunity, which is characterized in that it is prepared from the following parts by weight of raw materials:
    豆饼50-60份、蚕蛹粉10-15份、酒糟1-10份、大麦粉5-10份、玉米粉20-30份、麸皮5-10份、红薯粉5-10份、槐树叶粉5-10份、血粉1-5份、大豆卵磷脂3-5份、磷酸氢钙3-6份、松花粉5-10份、阿魏酸2-4份、小球藻冻干粉5-10份、脱脂磷虾粉2-4份、天蚕素抗菌肽1-2份、丙酸钠0.1-1份。50-60 portions of bean cake, 10-15 portions of silkworm pupa powder, 1-10 portions of vinasse, 5-10 portions of barley flour, 20-30 portions of corn flour, 5-10 portions of bran, 5-10 portions of sweet potato flour, locust leaf powder 5-10 parts, blood meal 1-5 parts, soy lecithin 3-5 parts, dicalcium phosphate 3-6 parts, pine pollen 5-10 parts, ferulic acid 2-4 parts, chlorella freeze-dried powder 5- 10 parts, 2-4 parts of defatted krill powder, 1-2 parts of cecropin antimicrobial peptide, and 0.1-1 parts of sodium propionate.
  2. 如权利要求1所述的提高鱼类免疫力饲料，其特征在于，由以下重量份的原料制备而成：The feed for improving fish immunity according to claim 1, characterized in that it is prepared from the following raw materials by weight:
    豆饼55份、蚕蛹粉12份、酒糟4份、大麦粉8份、玉米粉25份、麸皮8份、红薯粉8份、槐树叶粉5份、血粉5份、大豆卵磷脂5份、磷酸氢钙3份、松花粉6份、阿魏酸4份、小球藻冻干粉5份、脱脂磷虾粉2份、天蚕素抗菌肽1.5份、丙酸钠0.2份。55 portions of bean cake, 12 portions of silkworm pupa powder, 4 portions of distillers grains, 8 portions of barley flour, 25 portions of corn flour, 8 portions of bran, 8 portions of sweet potato flour, 5 portions of locust leaf powder, 5 portions of blood meal, 5 portions of soy lecithin, phosphoric acid 3 parts of calcium hydrogen, 6 parts of pine pollen, 4 parts of ferulic acid, 5 parts of chlorella lyophilized powder, 2 parts of defatted krill powder, 1.5 parts of cecropin antimicrobial peptide, and 0.2 parts of sodium propionate.
  3. 如权利要求1所述的提高鱼类免疫力饲料，其特征在于，由以下重量份的原料制备而成：The feed for improving fish immunity according to claim 1, characterized in that it is prepared from the following raw materials by weight:
    豆饼50份、蚕蛹粉15份、酒糟2份、大麦粉10份、玉米粉20份、麸皮5份、红薯粉5份、槐树叶粉5份、血粉2份、大豆卵磷脂3份、磷酸氢钙3份、松花粉5份、阿魏酸2份、小球藻冻干粉10份、脱脂磷虾粉4份、天蚕素抗菌肽2份、丙酸钠0.5份。50 portions of bean cake, 15 portions of silkworm pupa powder, 2 portions of distillers grains, 10 portions of barley flour, 20 portions of corn flour, 5 portions of bran, 5 portions of sweet potato flour, 5 portions of locust leaf powder, 2 portions of blood meal, 3 portions of soy lecithin, phosphoric acid 3 parts of calcium hydrogen, 5 parts of pine pollen, 2 parts of ferulic acid, 10 parts of lyophilized chlorella powder, 4 parts of defatted krill powder, 2 parts of cecropin antimicrobial peptide, 0.5 parts of sodium propionate.
  4. 如权利要求1所述的提高鱼类免疫力饲料，其特征在于，所述小球藻冻干粉的制备方法如下：The feed for improving fish immunity according to claim 1, wherein the preparation method of the freeze-dried chlorella powder is as follows:
    S1：将小球藻用清水洗净后阴干表面水分，放入榨汁机中榨汁，榨汁后滤布抽滤、微滤膜过滤后的滤液与缓冲液混合；S1: Wash the chlorella with clean water and dry the surface moisture in the shade, put it in a juicer to squeeze the juice, after squeezing the juice, filter the filter cloth, filter the filtrate with the microfiltration membrane and mix with the buffer;
    S2：将溶液于零下35℃的真空冷冻干燥机中常压放置10-20h，抽真空至真空冷冻干燥机的真空度为-0.08MPa，此时开始升温，升温速度为2-4℃/min，待温度为零下5℃时，保温4-6h，再自然升温至室温，静置5-10h后，恢复常压，抽滤，真空低温干燥即可。S2: Place the solution in a vacuum freeze dryer at minus 35°C at normal pressure for 10-20 hours, and then vacuum until the vacuum of the vacuum freeze dryer is -0.08MPa. At this time, the temperature starts to rise, and the temperature rise rate is 2-4°C/min. When the temperature is minus 5℃, keep it for 4-6h, then naturally warm up to room temperature, after standing for 5-10h, return to normal pressure, suction filtration, and vacuum low-temperature drying.
  5. 如权利要求4所述的提高鱼类免疫力饲料，其特征在于，1L缓冲液中含有1mol/L的氢氧化钠8-10mL，浓磷酸1-1.5mL，其余为生理盐水。The feed for improving fish immunity according to claim 4, wherein the 1L buffer contains 8-10mL of 1mol/L sodium hydroxide, 1-1.5mL of concentrated phosphoric acid, and the rest is normal saline.
  6. 如权利要求4所述的提高鱼类免疫力饲料，其特征在于，S1中滤液与缓冲液混合的体积比为1:1-1.5。The feed for improving fish immunity according to claim 4, wherein the mixing volume ratio of the filtrate and the buffer in S1 is 1:1 to 1.5.
  7. 如权利要求1-6中任一项所述的提高鱼类免疫力饲料的制备方法，其特征在于，具体如下：The preparation method of feed for improving fish immunity according to any one of claims 1 to 6, characterized in that the specifics are as follows:
    S1：将豆饼、蚕蛹粉、酒糟、大麦粉、玉米粉、麸皮、红薯粉、槐树叶粉、血粉、磷酸氢钙、松花粉分别烘干至含水量10％以下后加入到混料机中混合均匀；S1: Dried bean cake, silkworm pupa flour, distiller's grains, barley flour, corn flour, bran, sweet potato flour, locust leaf powder, blood meal, dicalcium phosphate, and pine pollen to the water content below 10% and then added to the mixer well mixed;
    S2：再将混合物料送入调制器中，熟化处理5-10min；S2: Send the mixed material to the modulator and mature for 5-10min;
    S3：将熟化后的物料送入膨化机中，再将大豆卵磷脂加入，进行膨化处理，膨化后的物料冷却后烘干至含水量5％以下，利用粉碎机粉碎并过80目筛网，加入到混料机中再将阿魏酸、小球藻冻干粉、脱脂磷虾粉、天蚕素抗菌肽、丙酸钠加入混合均匀后造粒即可。S3: Send the matured material into the extruder, and then add soybean lecithin for extruding treatment. After the extruded material is cooled, it is dried to a moisture content of less than 5%, crushed by a crusher and passed through an 80-mesh screen. Add the ferulic acid, chlorella freeze-dried powder, defatted krill powder, cecropin antimicrobial peptide, and sodium propionate into the blender, then mix and granulate.
  8. 如权利要求7所述的提高鱼类免疫力饲料的制备方法，其特征在于，熟化处理时的温度为140-150℃，蒸汽压力为0.4-0.6MPa。The method for preparing a feed for improving fish immunity according to claim 7, wherein the temperature during the maturation treatment is 140-150°C, and the steam pressure is 0.4-0.6 MPa.
PCT/CN2020/096421 2019-12-31 2020-06-16 Feed for improving fish immunity and preparation method therefor WO2021135088A1 (en)

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CN116098245A (en) * 2022-12-30 2023-05-12 大愚科技(湖州)有限公司 Special functional compound feed for cheilinus fasciatus and application of special functional compound feed

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