KR20160081870A - the gamichunggisan composite with the fuction of antioxidation and antiphlogistics - Google Patents

Abstract

The present invention relates to a medicinal composition having anti-oxidative and anti-inflammatory effects. More particularly, the present invention relates to anti-oxidative and anti-inflammatory composition extract from a mixture of licorice, Platycodonis Radix, Platycodon grandiflorum, Aralia continentalis Kitagawa, peppermint, Saposhnikovia divaricata Schischkin, Cicadidae Periostracum, or the like, and a pharmaceutical composition comprising the same. The present invention provides an anti-oxidative and anti-inflammatory composition obtained by extraction of a mixture of licorice, Ostericum Koreanum, Platycodonis Radix, Aralia continentalis Kitagawa, peppermint, Saposhnikovia divaricata Schischkin, Cicadidae Periostracum, Bupleurum falcatum, ginseng, Cnidium officinale Makino, Poria cocos, Anthriscus sylvestris, Poncirus trifoliata, Hetaeria sikokiana, Schizonepeta tenuifolia, Betula platyphylla var. japonica and Dictamnus dasycarpus. In addition, the present invention provides an anti-oxidative and anti-inflammatory composition obtained by extraction of a mixture of 100 parts by weight of licorice, 80-120 parts by weight of Ostericum Koreanum, 80-120 parts by weight of Platycodonis Radix, 80-120 parts by weight of Aralia continentalis Kitagawa, 80-120 parts by weight of peppermint, 80-120 parts by weight of Saposhnikovia divaricata Schischkin, 80-120 parts by weight of Cicadidae Periostracum, 80-120 parts by weight of Bupleurum falcatum, 80-120 parts by weight of ginseng, 80-120 parts by weight of Cnidium officinale Makino, 80-120 parts by weight of Poria cocos, 80-120 parts by weight of Anthriscus sylvestris, 80-120 parts by weight of Poncirus trifoliata, 80-120 parts by weight of Hetaeria sikokiana, 80-120 parts by weight of Schizonepeta tenuifolia, 180-240 parts by weight of Betula platyphylla var. japonica and 180-240 parts by weight of Dictamnus dasycarpus. The present invention also provides a pharmaceutical composition comprising the anti-oxidative and anti-inflammatory composition.

Description

[0001] The present invention relates to a composition for the treatment of antioxidants and antiphlogistics,

The present invention relates to a pharmaceutical composition which is effective for antioxidant and antiinflammation, more specifically, a composition which is obtained by mixing licorice, ganghyeol, gyeonggyeong, simplex, peppermint, windshield, ≪ / RTI >

The oxidation of the human body is due to free radicals, and the free radical theory was proposed by D. Harman in the mid-1950s. Free radicals are produced by the action of leukocyte phagocytosis, the action of electron transport system, myeloperoxide (MPO) during the production of ATP in mitochondria, ultraviolet rays, tobacco, normal metabolic processes, stress, pollutants and bacteria When the radicals remain in the body due to the cause, they cause various problems such as wrinkle formation, skin cancer, cell death, rheumatoid arthritis, atopic dermatitis, acne and the like through cell destruction, connective tissue cleavage and cross- . Superoxide dismutase (SOD), catalase, vitamin E, vitamin C, and ubiquinol, which are natural antioxidants (radical scavenging agents), exist in the human body to remove free radicals. The antioxidant system begins to decline gradually due to age, pollution, ultraviolet rays, stress, etc., and the antioxidant system is disabled, leading to an increase in free radicals in the body.

The increased radical destroys collagen (collagen), elastin, and hyaluronic acid, which are connective tissues of the dermis, causing skin settlement (wrinkles), oxidizing the lipid part of the cell membrane and destroying the cell, , Skin cancer and the like. In addition, this radical participates in the spontaneous oxidation reaction during the melanin formation process, and causes the cause such as stain, freckles, and wrinkles.

In addition, the reduction of inflammation can be measured by a reduction in the density of inflammatory cell types, such as monocytes or astrocytes, neutrophils, mast cells, and basophils. The reduction of inflammation can also be measured by measuring neutrophil activity (Jones et al., 1994). Factors such as frequency of mast cell degranulation or measurement of histamine levels or levels of reactive oxygen species can also be used as a measure of inflammation reduction. Levels of inflammation may also be determined by PCR using specific genes such as interferon-alpha, -beta and -gamma, tumor necrosis factor-alpha, interleukin 1 beta, -2, -4, -5, -6, -8, -18, -23, -27, CD4, CD28, CD80, CD86, MHCII, and iNOS. Measurement of tissue and / or patient fluid, such as plasma proinflammatory cytokines, may be a measure of inflammation reduction.

Such treatment of inflammation or the spread of a medicament having an anti-inflammatory effect has been problematic in that it induces toxicity to its own cells in the case of human or in the case of medicine.

Therefore, the development of anti-inflammatory agents using natural materials is greatly promoted.

The composition disclosed in Japanese Patent Application Laid-Open No. 10-2010-0000728 (composition for external application for antiinflammation or wound healing containing herbal medicine extracts, hereinafter referred to as "prior art") refers to a composition comprising at least one herbal medicine extract of chrysanthemum, An antiinflammatory composition containing at least one of the extracts of Chrysanthemum morifolium extract, Quercus mongolica extract, Quercus gamma extract or Chrysanthemum morifolium extract and a mixture of the herbal medicine extract and Fogue extract as an active ingredient, Composition "

The above prior art and prior art still show toxicity to cells and cause side effects. Therefore, the present invention provides a natural extract composition having antioxidant and anti-inflammatory functions that exclude the toxicity of cells to the maximum.

The present invention also provides a natural extract composition having antioxidant function and anti-inflammatory function

In order to solve the above problems and needs,

The present invention provides a composition having antioxidative and antiinflammatory properties extracted from a mixture of licorice, ganghyeol, gyeonggyeong, simplex, peppermint, windshield, depressed ginseng, ginseng, astragalus, .

The present invention also relates to a method for producing a liquorice, which comprises 80 to 120 parts by weight of hardiness, 80 to 120 parts by weight of hardness, 80 to 120 parts by weight of simplex, 80 to 120 parts by weight of mint, 80 to 120 parts by weight of windshield, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng root, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, The present invention provides a composition having an antioxidative and anti-inflammatory effect extracted from 80 to 120 parts by weight of mold, 180 to 240 parts by weight of flour, and 180 to 240 parts by weight of white line.

The present invention also provides pharmaceutical compositions comprising the above antioxidant and anti-inflammatory compositions.

The antioxidant and anti-inflammatory compositions according to the present invention produce toxic effects on the cells, and the problem of side effects is remarkably eliminated.

In addition, the composition having an antioxidative and anti-inflammatory effect according to the present invention has a high radical scavenging function and a markedly high antioxidative effect.

In addition, the antioxidant and antiinflammatory composition according to the present invention shows a remarkable effect on the anti-inflammatory effect, which has a function of reducing the inflammatory cytokine.

1 is a Raw 264.7 cytotoxicity result of GCE (gum arabic acid composition) according to the present invention.
Figure 2 shows DPPH radical scavenging results of GCE according to the present invention.
3 shows the ABTS radical scavenging results of GCE according to the invention.
4 is a graph showing ROS reduction rate results of a GCE according to the present invention.
5 is a graph showing the NO reduction rate results of GCE according to the present invention.
FIG. 6A is a graph showing the expression of LPS-induced IL-1β in Raw 264.7 cells by GCE according to the present invention
6B is a graph showing the expression of LPS-induced IL-6 in Raw 264.7 cells by GCE according to the present invention
FIG. 6A is a graph showing the expression of LPS-induced TNF-? In Raw 264.7 cells by GCE according to the present invention
Fig. 7 is a view showing the base sequence of the primer used in this experiment. Fig.

BRIEF DESCRIPTION OF THE DRAWINGS FIG.

The present invention relates to a composition having antioxidative and antiinflammatory properties extracted from a mixture of licorice, ganghyeol, gyeonggyeong, virginia, peppermint, windbreak, suppression, Seoho, ginseng, astragalus, .

Preferably, 80 to 120 parts by weight of hardness, 80 to 120 parts by weight of gum, 80 to 120 parts by weight of chewing gum, 80 to 120 parts by weight of peppermint, 80 to 120 parts by weight of windmill, 80 to 120 parts by weight of windmill, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of red ginseng, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, 120 to 120 parts by weight of a mold, 80 to 120 parts by weight of a mold, 180 to 240 parts by weight of a flour, and 180 to 240 parts by weight of a white line, to provide an antioxidative and anti-inflammatory composition.

The present invention relates to such a composition as a gum arabic acid composition (GCE).

The present invention also provides a pharmaceutical composition comprising a composition (GCE) effective for the above-mentioned antioxidant and anti-inflammation.

The above pharmaceutical composition means a drug for prevention and treatment of antioxidative function and inflammation and means that the composition of the present invention (GCE) is included therein.

The above-mentioned licorice is called ural licorice and curly licorice. It grows in Russia (Siberia), Iran, Afghanistan, Pakistan, China (Gansuk province, Shin Kangseong), Mongolia and cultivated in Korea. European licorice is distributed in Southern Europe, Central Asia and China. As a variant of European licorice, there are Russian licorice, Persian licorice, and Iran licorice, but not medicinal.

An old lawmaker was rumored to be well - healed, and the patients were pushed back, but the visit was too frequent for the patient to wait. The wife of the senator worried about the patients who were waiting and found a pile of pudding which he accidentally tried to use as a firewood in the kitchen and tasted it. She used all herbs as medicines. She thought that this herb would work and gave it to the patients. Later, when the clinic checked their symptoms, they found that each symptom had an effect. In addition, it harmonizes all medicines with the medicinal effect, and it is also called the national aged in the sense of the master of the country and the master of the king.

Licorice smells unusual and has a sweet taste. [甘 平]

Licorice harmonizes the toxicity of all medicines and makes the medicinal effect appear, regulates the fever and morale of the book, makes good communication of all blood vessels, and strengthens the muscles and bones.

Pharmacological action is effective in detoxification, hepatitis, urticaria, dermatitis, eczema. Jinhae, geomung, muscle relaxation, diuretic, anti-inflammatory action and suppress peptic ulcer.

Licorice crust is reddish brown or dark brown with vertical wrinkles and occasionally shrubs, buds and scales. The outer skin of the peeled licorice is pale yellow and fibrous.

This medicine is also called kokoro (chrysanthemum), miko, citron, wax, young tong, keiko, and 草 草.

The above-mentioned ganghyeol uses roots and roots of Ostericum Koreanum, Notopterigyum incisum (羌 活), and Notopterygium forbesii Boiss (寬 葉 羌 活) in Korea, and Chinese roots Notopterygium incisum: 羌 活) or notopterygium forbesii Boiss (寬 葉 羌 活).

In the old book, a single stem stretches straight up and can not be shaken by the wind, so it is called singularity, and it can not be shaken when the wind blows, but when it is not windy it moves alone and it is called Dokulcho (獨 草 草). It is said that it is good to fly from the river and call it Ganghwam (羌 活). However, it is good not to use tinnitus because it is easy to be confused because it has no appearance or effect with ganghwam (mountain type).

Ganghwam has a distinctive odor, weakness is sweet and cool, and a little bit behind. [甘苦]

Chinese ganghwam has an unusual scent, a little spicy,

The weakness of the houseplant Kanghyeon is a little bit light and relatively light. [苦 平]

It is used for exertional headache, chills and fever. It is used for arthralgia, muscle spastic paralysis, shoulder baggage, guanhwasa, skin ulcer and chills, fever and pain at the early stage. Pharmacological action, such as fever, pain, skin fungus suppression, cardiomyocyte blood flow increases.

The name Gikyeong mentioned above is attached because it is hard and straight.

This medicine has a little smell, taste is very high, and the quality is flat without any bias. [苦辛 平]

Gyungyung works on the lungs to treat symptoms with a lot of seawater and sputum, uncomfortable breathing, clearing the lungs, releasing the stiff chest, releasing cold air in the stomach, stopping the cough and removing the wall. I do not see my urine, I have generalized edema, and even when the amount of urine is small.

Sore throat, cold cough, sputum, nasal congestion, asthma, bronchial inflammation, pleurisy, headache, chills and tonsillitis.

The pharmacological action was reported to be a gadam action, a hypoglycemic action, a cholesterol lowering action, and an inhibiting action of improving bacteria.

Appearance is irregularly thin, long fusiform or conical, occasionally branched and grayish brown, pale brown or white on the outside. At the top of the straight root, the stalks remain depressed, and there are horizontal wrinkles, vertical grooves, and some curves in the vicinity. In most of the roots except the upper part, there are rough vertical creases and horizontal grooves, and there is a horizontal line of shrubs. The quality is hard but easy to break. The folded side is not fibrous and sometimes has large gaps.

Other names include Giligyeong, Gyeongcho, Gamyeong, Gamgikyeong, Reiji, 房 圖, 白药, 扈 扈, .

Medicinal herbs made using the roots of Aralia continentalis Kitagawa (Korea). Chinese writes Angelica pubescens Maxim f. Biserrata Shan et Yuan: 重 齒 毛 当當. Japan uses the roots of Aralia cordata Thunb. As a solitary activity.

The above-mentioned mints are also known as yomisomaki, bunchae, indancho, and mint. It grows in damp fields. It is 60 ~ 100cm high. The stem has a square cross section and hairs on the surface. The leaves are faced with a leaf with a bag, and the edges are serrated. On the surface of the leaf there is an oil gland where the oil is secreted. Most of the essential oil is stored in this oil gland.

In summer, in the fall, the upper petiole of the stem has a thin purple flower with a spike shape on its armpits. There are 4 stamens, 1 pistil is divided into 2 ends and 4 ovaries. The flowers bloom mainly in the morning, but the pistil is corrected in 3-4 days after flowering, and the operation is corrected in 2-3 days. Seeds are egg-shaped, light brown, light and small, and germinate well at 20 ℃ → 15 ℃.

The main ingredient of peppermint is menthol, and this menthol is medicinal use for the application agent, the analgesic agent, the stimulant, the noodle, the insect repellent, the toothpaste, the jam, the candy, the cosmetics, the cigarette and the refreshing agent and the fragrance.

The above-mentioned windshield is a medicinal substance which is very important in the treatment of windbreak (winds disease), which means to block the wind. The word "屛 風" is also used in the same way as tinnitus. It is likened to an orchid and a fragrant wormwood because of its strong fragrance.

This medicine has a peculiar smell and the medicine is spicy and warm. [辛甘 温]

The windshield described above is effective for all kinds of winds such as external headache, chills, fever, whole body, sore throat. It is also used for painful limb pain, tetanus, muscle spasms, paralysis due to paralysis, paralysis, itchy skin, and ringworm.

The pharmacological effects of antioxidants, anti-inflammation, anti-inflammation, immunity, antiallergic, anti-ulcer, antibacterial,

The above-mentioned retirement is a cryptotympana pustulata's transgression. It eliminates the wind heat of the upper chest, eliminates rashes, improves the throat, eliminates the neck, opens the jury, It is a medicinal product that has the effect of spreading, stopping the game, cooling the lungs (heat of the lungs) and adding sunshine (beneficial effect).

Although the above mentioned purple roots were called purple, it was gradually called chrysanthemum (柴胡) instead of chrysanthemum (艸). Sehho comes out of the mountains, and young shoots can be used as a trolley for livestock. Therefore, the buds were named as 蒿 蒿,), and 草, and their roots were named 柴 胡.

The son of Jinsa, who had the old Hu (胡) 's castle, became sick and tired and shivered all over his body and became ill with sweat and pouring rain. After hearing that the sick who suffered from the same disease was cured by the grass used for the firewood, he also treated it with the grass and gave birth to a son, and the name of the grass was used as a firewood. ), And it is said that it was called "Chi-ho".

This medicine has a peculiar smell, flavor is used and its quality is cool [苦 凉]

The above-mentioned ginseng refers to a root of ginseng (Panax ginseng C. A. Meyer) in Korea and Japan and the removal of the thin roots and the cork layer. In Korea, white ginseng (raw), red ginseng (red ginseng: steamed) and ginseng (蔘 蔘: thin roots) are recorded separately. In the private sector, wild ginseng camphor and wild ginseng are distinguished. In China, it refers to the roots and rootstocks of ginseng and distinguishes it from raw ginseng (red ginseng), red ginseng and wild ginseng (wild ginseng).

The above-mentioned cinnabar is Chinese origin and is cultivated as a medicinal plant. It is 30 ~ 60cm in height, hollow inside and branch slightly. Leaves are alternate, two-folded three-queen-folded compound leaf, and the forked pieces are egg-shaped, with a slightly deep sawtooth. The leaves of the root and the bottom have long petiole, and the lower part forms a sheath, wrapping the stem.

Flowers bloom from August to September, white and double-inflorescence. There are 5 petals and the inside is dry. There are 5 stamens and 1 pistil. The guns and the guns are 5 ~ 6 each in the shape of a line. The fruit is open but not mature. The nodules in the ground are like a lump of 5-10cm in length and 3-5cm in diameter and have a strong smell.

It has efficacy for sedation, analgesia, tonic, etc. and is used to treat headache, anemia, and women's diseases. Remove the leaves and stems from September to November, remove the stems, and dry them in the sun, then use 3 to 6g dalyeoseo, or use as a pill or powder.

As mentioned above, the tinnitus of the bokryeong is Buddhism, Buddhism, Pine, and Immortality. What is wrapped around the roots of the tree is called 伏 神 (福 神).

The 气味 of the old pine root, which is cut by the woodcutter, does not disappear in a state of being tingled and the essence (period) does not come out. If the essence of pine tree is strong, it will be taken out and gathered to become 茯? Because it leaves the original tree without wrapping the roots, the name "" has emerged in the meaning of the remainder (零). If 津 气 (ginseng, essence of tree) is not strong, it will be formed around the root of the tree, so it will not fall off from the root but will cover the root. Therefore, it is said to be goddess.

茯 靈 (茯 霊) is a 龜 龜 (龜 記) 龜 龜 龜 龜 龜 龜 龜 龜 龜........ Perhaps because the spirit of the pine tree penetrated into the earth and gathered together, it would have been called the Buddhist spirit, the Buddhist god. In <仙 經>, it was said that if you wear a fist size fist, you will defeat all ghosts and disasters. The names of 伏 霊 and 伏 神 come from the spirit spirit He is convinced.

It seems that there was a mistake in the process of transcribing what the general person writes as the word. It is also known as the 伏 兎 (ground) because there is a Buddhist spirit under the ground and earth 子 子 (earth) on the earth.

The abovementioned references refer to the roots of Angelica decursiva (Miq.) Franch. & Sav (紫花 前 胡) or Peucedanum praeruptorum Dunn (白花 前 胡) in Korea and Japan. China acknowledges that it is the only prefecture in China.

In Korea, Anthriscus sylvestris (L.) Hoffm. Is distributed, and most of them are produced in Daejangdo Island, Shinan County in Chonnam. The body herb is also called the former egg and grows in the mountains of various parts of Korea, but is not cultivated.

This medicine has an unusual direction, the taste is very high and the quality is a little cold. [苦辛 微寒]

The former is used for asthma with accumulated tears, symptoms of chest tightness, no sputum, fever, seawater, and headache.

The crust is cultivated in the southern part of Korea. The medicinal ingredient contains essential oils and flavonoids. Neohesperidin, Naringin, and Vitamin C are contained in citrus trees. The flavor is a little spicy and the fever is cool.

The efficacy reduces the energy while showing a giant effect. Therefore, there is a wall in the upper part of the chest and abdomen, and it is used for symptoms that appeal to the briskness. For example, it causes a psychological shock to the nervousness, and it gives the chest a cool feeling by lowering the energy down. And because it shows the digestive process of the digestive system, it is affected by sore symptoms, areas, etc. with side effects.

It is also used when it feels heavy and burning due to dysentery and aftereffects. It is also used for dermatitis and itching, and responds to dissolution. In addition, the lungs are excessively stagnant in the water, and the lungs and hemorrhoids are also heated up by heating them. In addition, it has an effect on esophagus and helps to treat constipation.

Recent clinical results have shown that contraction of the uterus in the gastrointestinal tract can restore normal function. In addition, it is also effective in indigestion, gastric enlargement, and colon due to febrile convulsions.

4 ~ 12g is applied once, but when used alone, the maximum amount is 80g once. This tab should be avoided for pregnant women.

The above-mentioned horse chestnut refers to the gut root of Gastrodia elata Blume in Korea. It is the same in China and Japan.

This medicine has a weak smell, a taste, and the quality is flat without being shifted to one side. [甘 平]

Chimma acts on the liver and is used for seizures, tetanus, tidal velocities, dizziness, headache, nervous breakdown, and headache.

The pharmacological effects have been reported to be sedative, anticonvulsant, analgesic, anti-inflammatory, increased heart and cerebral blood flow, hypotension, increased antioxidant activity, and immune activation.

Its appearance is slightly curved and pushed to the circumference and its outer surface is pale yellowish white or tan, with irregular vertical wrinkles and rounded nodes. The vagina is firm and the folded side is yellowish brown or dark brown, glossy and horny.

Other names include 鬼 之 宇,), 초 草, a mother, 적 箭 적, red cell), 풍 초 ( ), Ryeol (摘 草), myeonchinma (水 天 麻), and water cane (水 芋) are.

The other names of the above mentioned types are Gassho, Signature, and Rice. Lamiaceae and plant-shaped openings (list price) Schizonepeta tenuifolia Briq. Or dried up the outpost of Nepeta japonica Maxim. It is planted in various places. When the flower is in summer, it is spread out and dried in the shade where the wind is well. The taste is spicy and the quality is warm. It acts on menopause (经 经) and liver (肝 经). It sweats, removes the wind cold and removes the ebony blood. Charging stops bleeding. In the pharmacological experiments, it was found that the hot water treatment showed weak antipyretic action, promoting sweat secretion, accelerating the blood circulation in the salivary blood line, gyeonggyeong, digestion, and antibacterial activity.

My head is sore because I have a bad reputation (风寒 表 证), a wind fever (wind heat infection) and a wind cold. I can not open my mouth, but my neck and body become stiff. Facial nerve paralysis, pharyngitis, It writes on the back. Charring is spewing blood, and it is used for epistaxis, intestinal bleeding, uterine bleeding, dysentery. 4.5 ~ 12g per day in the form of candy, powder and pill are eaten. When used as an external medicine, it is glued or powdered, put on a foundation (medicinal) agent, and washed with water. I do not use it to sweat a lot. The root of the mold [头切 根 (芥出 根)] also spits blood, toothache, ginga (珠 瘡)) is written.

The above-mentioned husk is also used as a shell of chestnut tree to make a bow, and it is also used as a medicinal herb in a herb, a pox, or a poison.

Scientific name Betula platyphylla var. japonica, Betula platyphylla. It is a medicinal substance effective for jaundice, dysentery, abdominal pain and diarrhea caused by moist heat.

The above-mentioned white pine bloom refers to the root bark of white pine (Dictamnus dasycarpus Turcz.) In Korea. In China, it was the same as our country, and in Japan, it was not treated as a process medicine.

In the present invention, it is preferable to dry and use the above-mentioned material, but it is also possible to use it without drying.

In the present invention, as a method of extracting a composition by mixing the above-mentioned materials, a method may be used in which water is mixed with a mixed material, followed by immersion and heating to obtain an extract.

The water may be used in an amount of 5,000 to 50,000 parts by weight based on 100 parts by weight of licorice, but is not limited thereto.

The present invention is characterized in that the above materials are mixed and pulverized, refluxed in 80% ethanol at 75 to 85 ° C for 1.5 to 3.5 hours, filtered and concentrated under reduced pressure in a rotary vacuum evaporator to prepare an extract .

Further, the concentrated solution may be lyophilized with a freeze dryer to obtain a concentrated powder.

The above-mentioned concentrated powder may be dissolved in distilled water.

The present invention relates to a method for producing a liquorice, which comprises 80 to 120 parts by weight of hardness, 80 to 120 parts by weight of a hardy, 80 to 120 parts by weight of a sweetener, 80 to 120 parts by weight of mint, 80 to 120 parts by weight of wind, 80 to 120 parts by weight, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of relative gestalt, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of crust, 80 to 120 parts by weight of ginseng, (GCE) composition having an antioxidant activity and an anti-inflammatory effect extracted from 80 to 120 parts by weight, 180 to 240 parts by weight of flour and 180 to 240 parts by weight of white flour.

The present invention shows that the composition is effective for antioxidant and anti-inflammation through the following examples.

<Examples>

1. Preparation of Extraction Compositions

4 g of ginseng, 4 g of ginseng, 4 g of ginseng, 4 g of ginseng, 4 g of ginseng, 4 g of ginseng, 4 g of ginseng, 4 g of crustacean, 4 g of ginseng, 4 g of mint, 8 g of herbaceous earth , And 8g of white wire are mixed and prepared.

The materials prepared above were pulverized and mixed, and the mixture was refluxed at 80 ° C for 2 hours with 1.5 L of 80% ethanol, and the filtrate was concentrated under reduced pressure on a rotary vacuum evaporator. The concentrated solution was lyophilized with a freeze dryer to yield 9.4 g of powder with a yield of 11.84%. The powder was dissolved in distilled water and used for the study.

The powder may be diluted differently according to the purpose of the experiment, and generally 1 g of the powder may be dissolved in 50 to 500 ml of distilled water.

In the present invention, the concentrated solution or powder thus obtained is referred to as a gum arabic acid composition (GCE) as described above.

2. Experiment

(1) Cytotoxicity measurement

Raw 264.7 cells, a mouse-derived macrophage, were cultured in DMEM supplemented with 10% fetal bovine serum and 1% antibiotics (A / A) at 37 ° C and 5% CO2. The number of subcultures was used at least three times. Raw 264.7 cells were cultured in 96-well plates at a density of 2 × 10 4 cells / well for 24 hours, then treated with GCE at concentrations of 1, 10, and 100 μg / ml, respectively, for 24 hours. After incubation, 10 μl of MTT solution was added and incubated for 30 minutes. Absorbance was measured at 450 nm.

(2) Antioxidant efficacy measurement

1) Total polyphenol content measurement

0.5 ml of 50% Foiln-Ciocalteu's phenol reagent was added to 1 ml of GCE, and the mixture was reacted at room temperature for 3 minutes. The reaction solution was mixed with 1 ml of a saturated solution of Na2CO3 and 7.5 ml of distilled water, and the mixture was allowed to stand for 30 minutes, followed by centrifugation at 12,000 rpm for 10 minutes. The supernatant was transferred to a 96-well plate and absorbance was measured at 760 nm. The total polyphenol content of the sample was determined according to the calibration curve prepared using gallic acid as the reference material. GAE (gallic acid equivalent) / g was used as the measurement unit.

2) Measurement of ABTS radical scavenging rate

The ABTS solution was dissolved in 2.6 mM potassium persulphate to be 7.4 mM ABTS and allowed to stand in the dark for 16-24 hours at room temperature to form a cation (ABTS · +). The ABTS · + solution was diluted to 750 ± 0.7 with an absorbance value of 0.7 ± 0.03. Diluted ABTS · + solution and GCE were mixed at a ratio of 1:20 to 1, 10, 100, and 1000 (㎍ / ㎖), respectively, and allowed to react at room temperature for 10 minutes. After the reaction, the absorbance was measured at 734 nm. The free radical scavenging rate was calculated according to the following equation.

* Erase rate (%) = [1- (absorbance of control group - absorbance of sample addition bacteria) / (absorbance of control group)] 100

3) Measurement of DPPH radical scavenging rate

The DPPH solution was dissolved in ethanol to 0.2 mM. 0.2 mM DPPH solution and GCE were mixed at a ratio of 1: 2.5 to 1, 10, 100, and 1000 (ug / ml), respectively, and reacted in a dark room at 37 캜 for 30 minutes. After the reaction, the absorbance was measured at 517 nm. The free radical scavenging rate was calculated according to the following equation.

* Erase rate (%) = [1- (absorbance of control group - absorbance of sample addition bacteria) / (absorbance of control group)] 100

4) Measurement of ROS production in Raw 264.7 cells using DCF-DA

Raw 264.7 cells were seeded at 2 × 10 5 cells / well in a 12-well plate. After culturing for 24 hours, GCE was treated with a concentration of 1, 10, 100 (㎍ / ml) and 1 μg / ml of LPS, respectively, for 24 hours. After culturing, all cells were removed, and the collected cells were washed twice with cold PBS. Ten DCF-DA was added to the cells and stained in a dark place at 37 캜 for 15 minutes. After staining, the cells were washed with cold PBS, centrifuged at 1,200 rpm for 5 minutes, and the supernatant was removed. Then, 400 μl of PBS was added to suspend the supernatant. The changes of ROS production according to the intensity of fluorescence intensity were analyzed using flow cytometry analyzer.

5) Nitric oxide (NO) measurement

NO was measured using a griess reagent system. Raw 264.7 cells were plated at 2 × 10 4 cells / well in a 96-well plate and cultured for 24 hours at 37 ° C and 5% CO 2. After cultivation, GCE was treated at a concentration of 1, 10, 100 (㎍ / ml), respectively, and treated with a concentration of 1 μg / ml of LPS and cultured again for 24 hours. N1 buffer was added to each well of 50 μl of each well. After reacting at room temperature for 10 minutes, the N2 buffer was treated with 50 μl of each buffer and reacted at room temperature for 10 minutes. After the reaction, the absorbance was measured at 540 nm. The concentration of NO in the culture medium was determined using the standard curve of concentration of nitrite standard.

(4) Inflammatory cytokine measurement

1) Immunoassay

Luminex was used to measure inflammatory cytokines in the cells. Raw 264.7 cells were plated at 2 × 10 5 cells / well in a 12-well plate and cultured at 37 ° C and 5% CO 2 for 24 hours. After cultivation, GCE was treated at a concentration of 1, 10, 100 (㎍ / ml), respectively, and treated with a concentration of 1 μg / ml of LPS and cultured again for 24 hours. After centrifugation, IL-1β, IL-6 and TNF-α were measured as supernatants.

2) Reverse transcription polymerase chain reaction (RT-PCR)

PCR was performed using a mixture of RT premix kit (reaction buffer, dNTPs mixture, RNase inhibitor, stabilizer, oligo dT15 primer) at 45 ℃ for 60 min and 95 ℃ for 5 min. Respectively. RT-PCR was performed by adding each sample and primer to a mixture containing 250 mM dNTPs mix, RT buffer (10 mM Tris-HCl, pH 9.0, 30 mM KCl, 1.5 mM MgCl2) in 1 U / 7]. 2% agarose gel, and the degree of expression of IL-1β, IL-6, and TNF-α was confirmed by photographing with UV. (FIG. 7 shows the nucleotide sequence of the primer used in this experiment)

3. Results

(1) Effect on cell viability

As a result of confirming the cytotoxicity of GCE, when the control group was taken as 100%, it was not cytotoxic at a concentration of 1 to 100 μg / ml of GCE, and it was also considered to be safe in comparison with the cytotoxicity result of the existing natural product extract (Fig. 1).

(2) Effect on antioxidant efficacy

1) Total polyphenol content

The total polyphenol content of GCE was measured repeatedly more than 3 times, resulting in 46.622 ㎎ / g (Table 1).

Samples Total phenolics (GAE ^* ) / g ext. GCE 46.622 + - 0.571

[Total phenol content of GCE]

2) Effect on DPPH radical scavenging rate

The DPPH radical scavenging rate of GCE was measured to be 0.5% at the concentration of 1 ㎍ / ㎖ of GCE, 2.8% at the concentration of 10 ㎍ / ㎖, 38.8% at the concentration of 100 ㎍ / ㎖, 92.7% (Fig. 2).

3) Effect on ABTS radical scavenging rate

The ABTS radical scavenging rate of GCE showed 0.5% at 1 ㎍ / ㎖ concentration and 1.4% at 10 ㎍ / ㎖ concentration, 33.1% at 100 ㎍ / ㎖ and 89.4% at 1000 ㎍ / (Fig. 3).

4) Effect on ROS generation

The ROS reduction rate of GCE was 17.4% (P <0.01) at the concentration of 1 ㎍ / ㎖ of GCE, 20.9% (P <0.05) and 100 ㎍ / ㎖ at the concentration of 10 ㎍ / And 26.2% (P < 0.01), respectively, indicating a concentration-dependent decrease (FIG. 4).

5) Effect on NO production

The NO reduction rate of GCE showed a slight decrease of 6% at the concentration of 1 ㎍ / ㎖, but a significant decrease of 17.4% (P <0.01) at the concentration of 10 ㎍ / ㎖ when the control group was taken as 100% , And a concentration-dependent decrease of 37.6% (P < 0.001) at a concentration of 100 占 퐂 / ml (Fig. 5).

3. Influence on inflammatory cytokines

The amount of IL-1β produced in this experiment was 35.4% (P <0.05) at the concentration of 1 ㎍ / ㎖ of GCE, 52.4% (P <0.01) at the concentration of 10 ㎍ / (P <0.01), the concentration of IL-6 was decreased by 37.7% at the concentration of 1 ㎍ / ㎖, and the concentration of IL-6 was decreased by 10 ㎍ / (P <0.05) at 100 ㎍ / ㎖ and 70.5% (P <0.01) at the concentration of 100 ㎍ / ㎖, respectively. In addition, the amount of TNF-α was decreased by 2.7% at 1 μg / ㎖ and by 9.9% at 10 μg / ㎖ compared with the control, and 23.8% (P <0.05) (Fig. 6-C). Measurement of IL-1β, IL-6 and TNF-α expression level at RNA level also showed a concentration-dependent decrease (FIGS. 6A, 6B, 6C).

Thus, the present invention provides a composition for extracting gum arabic acid and a pharmaceutical composition containing the same, wherein the gum arabic extract has a remarkably high antioxidant and anti-inflammatory function without cytotoxicity.

The present invention is very useful in an industry that produces, processes, distributes and sells functional foods, medicines, etc. that perform antioxidative and anti-inflammatory treatment and prevention functions using herbal medicines and herbs.

Claims (2)

A composition having an antioxidant and antiinflammatory activity extracted from a mixture of licorice, ganghwamun, gyeonggyeong, cheonghwamun, mint, windshield, depressed mildew, ginseng, ginseng, astragalus,
The method according to claim 1,
80 to 120 parts by weight of ginger, 80 to 120 parts by weight of ginger, 80 to 120 parts by weight of chewing gum, 80 to 120 parts by weight of peppermint, 80 to 120 parts by weight of windshield, 80 to 120 parts by weight of ginger, 80 to 120 parts by weight of ginger, 120 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, 80 to 120 parts by weight of ginseng, By weight, 180 ~ 240 parts by weight of flour, 180 ~ 240 parts by weight of white flour, and the composition is effective for antioxidative and antiinflammation.

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