KR20160069201A - Preservation Agent using Phytoncide extracted from Cone of Korean Pine AND MANUFACTURING METHOD OF THE SAME - Google Patents

Preservation Agent using Phytoncide extracted from Cone of Korean Pine AND MANUFACTURING METHOD OF THE SAME Download PDF

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KR20160069201A
KR20160069201A KR1020140174874A KR20140174874A KR20160069201A KR 20160069201 A KR20160069201 A KR 20160069201A KR 1020140174874 A KR1020140174874 A KR 1020140174874A KR 20140174874 A KR20140174874 A KR 20140174874A KR 20160069201 A KR20160069201 A KR 20160069201A
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pine
alcohol
extract
improving agent
emulsifier
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KR1020140174874A
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Korean (ko)
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KR102389560B1 (en
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김배용
김세은
이한백
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(주) 피러스
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00—Preservation or chemical ripening of fruit or vegetables
    • A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D65/00—Wrappers or flexible covers; Packaging materials of special type or form
    • B65D65/38—Packaging materials of special type or form
    • B65D65/42—Applications of coated or impregnated materials
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
    • Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S426/00—Food or edible material: processes, compositions, and products
    • Y10S426/809—Food or edible material: processes, compositions, and products including harvesting or planting or other numerous miscellaneous processing steps

Abstract

Provided is a storability enhancer using an extract made from cones of Pinus koraiensis, which enhances the storability of fruits and vegetables. The storability enhancer is an alpha-amylase activity inhibitory substance comprising a Pinus koraiensis extract, beta-cyclodextrin, and an emulsifier. The alpha-amylase activity inhibitory substance comprises 0.002-3 W/V% of the Pinus koraiensis extract, 2-3 W/V% of the beta-cyclodextrin, and 0-1 W/V% of span 80 and 0-1 W/V% of tween 80 as the emulsifier. The alpha-amylase activity inhibitory substance can comprise an aqueous ethanol solution comprising water and ethanol mixed in the ratio of 2:1-1:1.

Description

잣나무 구과 추출물을 활용한 저장성 향상 제제 및 그 제조 방법{Preservation Agent using Phytoncide extracted from Cone of Korean Pine AND MANUFACTURING METHOD OF THE SAME}TECHNICAL FIELD The present invention relates to a preservative improving agent using a pine cone extract,

본 발명은 잣나무 구과 추출물을 활용한 저장성 향상 제제 및 그 제조 방법에 관한 것이다.The present invention relates to a preservative improving agent using a pine cone extract and a preparation method thereof.

과채류 등의 물품은 저장과 유통시에 호흡작용, 생장작용, 후숙작용, 증산작용 등의 유기활동을 계속하므로 탄사가스와 열에너지를 발생하게 되는데 이로이내 발생되는 탄산가스와 같은 부산물과 화학적 변화에 따른 반응으로 신선도와 중량의 변화 등이 발생한다. 또한 과채류에는 수분함량이 많기 때문에 곰팡이, 미생물, 병충해등의 발생 등으로 물품이 쉽게 변질되고 이에 따라 물품의 품질이 저하되게 된다. 이러한 문제점을 해결하기 위해서 일반적으로 물품을 저장하거나 유통시킬 때 저온에서 저장 유통시키는 저온저장법, 산소와 탄산가스 등의 가스조절을 조절하여 물품의 호흡과 화학변화에 의하여 발생하는 해로운 가스를 조절하는 자스 저장법, 고밀도 폴리에틸렌 필름으로 밀봉하여 상온하에서 저장하는 플라스틱 필름 저장법 등이 과채류의 신선도 유지기간을 연장하는 방법으로 적용하고 있는 실정이다.
Fruit and vegetable products continue to carry out organic activities such as respiration, growth, maturation, and transpiration during storage and distribution, and thus produce cadmium gas and thermal energy. Byproducts such as carbon dioxide gas and chemical changes Changes in freshness and weight occur due to the reaction. In addition, since the fruit and vegetables have a large amount of water, the product is easily deteriorated due to fungi, microorganisms, pests and the like and the quality of the product is deteriorated accordingly. In order to solve these problems, generally, a low-temperature storage method in which a product is stored or circulated at a low temperature when the product is stored or circulated, a jasmine that controls harmful gas generated by respiration and chemical change of the product by controlling gas control such as oxygen and carbon dioxide Storage method, a method of storing a plastic film which is sealed with a high-density polyethylene film and stored at room temperature, and the like are applied as a method for extending the freshness maintenance period of fruit and vegetables.

그러나 상기와 같은 방법은 특수한 장치가 별도로 필요할 뿐아니라 저장유통시 적용 대상물에 따라 적절한 방법으로 선택 사용 되어야 하며 고가의 비용이 발생할 뿐 아니라 물품의 신선도를 충분히 유지시킬 수 없는 문제점이 있었다. 따라서 과채류 등에 두루 적용 사용할 수 있으며 저가의 비용으로 용이하게 선도를 유지하여 더욱 장기간 보관 저장 유통할 수 있는 방법이 절실히 요구되고 있다. However, the above-described method has a problem that not only a special apparatus is separately required but also a method of selecting and using according to the object to be applied in the storage and distribution, an expensive cost is generated and the freshness of the product can not be maintained sufficiently. Therefore, there is a desperate need for a method that can be applied to fruit and vegetables, and can be kept at a low cost and easy to store and store for a longer period of time.

본 발명은 상기와 같은 문제점을 해결하기 위해 천연식물추출물에 함유되어 있는 항균성 물질의 우수한 효능에 착안하여 이를 연구한 것으로 과채류 등의 유통과 저장시 신선도가 유지되면서 장기간 보관할수 있게하는 천연 식물추출물을 이용한 과채류의 신선도 유지용 제품을 제공하는 것이다.In order to solve the above problems, the present invention focuses on the excellent efficacy of antimicrobial substances contained in natural plant extracts, and it is a study on natural plant extracts which can be stored for a long time while maintaining freshness during distribution and storage of fruit and vegetables. And to provide a product for maintaining freshness of fruit and vegetables used.

본 발명의 일 실시예에 따른 잣나무 구과 추출물을 활용한 저장성 향상 제제는 잣나무 오일 0.1%~10%, 알코올 20% 및 미량 첨가물을 포함하고, 제제의 총량이 100%가 되도록 증류수를 포함한다.According to one embodiment of the present invention, the preservative improving agent using the pine cones extract comprises distilled water such that the pine tree oil contains 0.1% to 10% of oil, 20% of alcohol and a trace amount of additives, and the total amount of the preparation is 100%.

상기 알코올은 폴리비닐알코올이고, 상기 미량 첨가물은 유화제, 키토산, 구연산, 캐스터 오일을 포함할 수 있다.The alcohol is polyvinyl alcohol, and the trace additive may include an emulsifier, chitosan, citric acid, and castor oil.

이러한 잣나무 구과 추출물을 활용한 저장성 향상 제제는 (가) 잣나무 오일을 유화제 및 알코올과 혼합하는 단계, (나) 키토산 용액을 추가로 투입하고 교반하는 단계, (다) 구연산 용액을 추가하고 고속 교반하는 단계, (라) 캐스터 오일을 넣고 교반하여 캡슐레이션하는 단계를 포함하는 방법을 통하여 제조된다..(A) mixing the pine tree oil with an emulsifier and alcohol, (b) adding and stirring the chitosan solution, and (c) adding a citric acid solution and stirring at a high speed And (d) encapsulating castor oil by stirring and stirring.

상기 (가), (나), (다), (라) 단계에서 투입하는 잣나무 오일, 유화제, 알코올, 키토산, 구연산, 캐스터 오일의 질량비는 10 : 0.5 : 1 : 0.9 : 0.7 : 1일 수 있다.The mass ratio of the pine tree oil, the emulsifier, the alcohol, the chitosan, the citric acid and the castor oil to be added in steps (a), (b), (c) and (d) may be 10: 0.5: 1: 0.9: 0.7: 1 .

본 발명의 일 실시예에 따른 포장 용지는 잣나무 오일 0.1%~10%, 알코올 20% 및 미량 첨가물을 포함하고, 제제의 총량이 100%가 되도록 증류수를 포함하는 잣나무 구과 추출물을 활용한 저장성 향상 제제를 함유한다.
The wrapping paper according to an embodiment of the present invention includes a pine tree extract containing distilled water so as to contain 0.1% to 10% of pine tree oil, 20% of alcohol and a small amount of additives, and the total amount of the preparation is 100% Lt; / RTI >

본 발명의 일 실시예에 따르면, 천연추출물을 주원료로 활용한 과채류의 저장성을 향상시키는 제제를 제공할 수 있고, 천연식물추출물이 갖고 있는 항균활성물질을 이용하여 농식품류의 유통과 저장시에 발생하는 곰팡이, 미생물, 병충해 등의 생장 발육을 억제하는 원리를 이용하여 장기간 보관에 효과를 나타내는 제제를 제공할 수 있다.
According to one embodiment of the present invention, it is possible to provide a preparation that improves the storage stability of fruit and vegetables using the natural extract as a main ingredient, and it is also possible to provide an antimicrobial active substance of a natural plant extract, It is possible to provide an agent exhibiting an effect on long-term storage using the principle of inhibiting growth and development such as fungi, microorganisms, and pests and diseases.

도 1은 본 발명의 일 실시예에 따른 잣나무 구과 추출물을 활용한 저장성 향상 제제의 제조 방법의 제1 단계 공정 순서도이다.
도 2는 본 발명의 일 실시예에 따른 잣나무 구과 추출물을 활용한 저장성 향상 제제의 제조 방법의 제2 단계 공정 순서도이다.1 is a flow chart of a first step of a method for manufacturing a preservation improving agent using a pine cone extract according to an embodiment of the present invention.
FIG. 2 is a flow chart of a second step of a method for manufacturing a preservative improving agent using a pine cones extract according to an embodiment of the present invention.

그러면 첨부한 도면을 참고로 하여 본 발명의 실시예에 대하여 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 상세히 설명한다. 그러나 본 발명은 여러 가지 상이한 형태로 구현될 수 있으며 여기에서 설명하는 실시예에 한정되지 않는다.Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

본발명의 일 실시예에 따른 잣나무 구과 추출물을 활용한 저장성 향상 제제는 주요성분으로서 잣나무 구과에서 추출한 식물성 오일 0.1%~10%, 알코올 20%, 기타 미량 첨가물을 포함하고, 제제의 총량이 100%가 되도록 나머지는 증류수로 구성되어 있다.The preservative improving agent using the pine cones extract according to one embodiment of the present invention contains 0.1 to 10% of vegetable oil extracted from pine cones, 20% of alcohol and other trace additives, and the total amount of the agent is 100% And the rest is composed of distilled water.

본발명의 일 실시예에 따른 잣나무 구과 추출물을 활용한 저장성 향상 제제에 사용되는 잣나무 정유는 수증기를 통하여 식물에 함유되어 있는 휘발성 오일이 수증기와 함께 추출되게 하고 물과 분리하여 회수하는 증류법을 이용하여 추출하였다.According to one embodiment of the present invention, the pine tree essential oil used in the preservative improving agent using the pine cone extract is prepared by extracting volatile oil contained in plants through water vapor and recovering it by separating it from water And extracted.

위와 같은 방법으로 추출한 잣나무 정유의 주요 성분은 테르펜 계통의 물질인 알파피넨, 베타피넨 등으로서 수증기 증류에 의해 쉽게 유출되고 방향성이 있으며 항균 및 살균 작용의 효증을 가지고 있어 곰팡이, 미생물, 병해충 등의 성장을 저해하는 특징이 있다.The major components of the essential oil extracted from the above-mentioned method are alpha-pinene and betapinene, which are terpene-based substances, and are easy to flow out due to steam distillation, have directionality, and have antibacterial and bactericidal action. Thus, growth of fungi, microorganisms, . ≪ / RTI >

단, 잣나무 정유는 휘발성이기 때문에 지속적 효과를 나타내기가 어렵다는 단점이 있다. 이를 해결하기 위해 마이크로 캡슐레이션 기술을 이용해 잣나무 정유를 담체화 하여 잣나무 정유의 서방성을 향상시켰다.
However, since the refined oil is volatile, it has a disadvantage that it is difficult to show a continuous effect. In order to solve this problem, microcapsulation technique was used to improve the releasing properties of Pine tree essential oil.

이러한 본 발명의 일 실시예에 따른 잣나무 구과 추출물을 활용한 저장성 향상 제제를 제조하는 방법을 설명한다.Hereinafter, a method for preparing a preservation improving agent using the pine cone extract according to one embodiment of the present invention will be described.

도 1은 본 발명의 일 실시예에 따른 잣나무 구과 추출물을 활용한 저장성 향상 제제의 제조 방법의 제1 단계 공정 순서도이고, 도 2는 본 발명의 일 실시예에 따른 잣나무 구과 추출물을 활용한 저장성 향상 제제의 제조 방법의 제2 단계 공정 순서도이다.FIG. 1 is a flow chart showing a first step of a method for preparing a preservative improving agent using a pine cone extract according to an embodiment of the present invention. FIG. 2 is a view illustrating a method of improving storage stability using a pine cone extract according to an embodiment of the present invention 2 is a flow chart showing the second step of the method for producing a preparation.

먼저, 도 1을 참고하면, 잣송이에서 잣을 탈각한 후 남은 부산물을 음지에서 건조한 후, 2~3cm 크기로 자르고, 잣나무 부산물 100g 당 증류수 500㎖의 비율로 증류조에 투입하고, 1~2분간 균질화한 다음 증류한다. 증류온도는 100ㅁ3℃로 유지하면서 더 이상 정유가 휘발되어 나오지 않을 때까지 3 시간 정도 증류시킨다. (S1) First, referring to FIG. 1, the pine nut was removed from the pine nut, and the remaining by-products were dried in the shade, cut into 2 to 3 cm size, put into a distillation tank at a ratio of 500 ml of distilled water per 100 g of pine wood by-product, And then distilled. Keep the distillation temperature at 100 ㅁ 3 ° C and distill for 3 hours until the essential oil no longer evaporates. (S1)

다음, 증류로 얻어 진 잣나무 오일에 황산나트륨을 넣고 24시간 탈수시킨 후 황산 나트륨을 분리하여 얻어 진 잣나무 추출물을 밀봉하여 냉장 보관한다. (S2) 탈수는 냉각을 통해 수분을 얼리고, 나머지 오일을 분리해 내는 방법으로 진행할 수도 있다.Next, sodium sulfate is added to the pine oil obtained by distillation, dehydrated for 24 hours, and sodium sulphate is separated. The resulting pine tree extract is sealed and refrigerated. (S2) Dehydration may be carried out by cooling water to freeze the remaining oil.

이어서, 도 2를 참고하면, 위의 제1 단계에서 수득한 잣나무 오일 (실험예: 10g)을 유화제인 스팬(span) 80(실험예: 0.5g) 및 4% 폴리비닐알콜(PVA)(실험예: 25g)과 혼합하고, 10분간 8,000rpm으로 고속 교반한다. 4% PVA 25g은 PVA 1g에 물 24g 넣고 60℃ 가열하여 얻는다.Next, referring to FIG. 2, the pine oil obtained in the first step (Experimental Example: 10 g) was mixed with an emulsifier span 80 (experimental example: 0.5 g) and 4% polyvinyl alcohol (PVA) For example, 25 g) and stirred at 8,000 rpm for 10 minutes at high speed. 25 g of 4% PVA is obtained by adding 24 g of water to 1 g of PVA and heating at 60 ° C.

다음, 3%의 키토산 용액(실험예: 30g)을 추가로 투입하여 10분동안 8,000rpm으로 고속 교반한다. 3% 키토산 용액 30g은 아세트산 0.45g, 키토산 0.9g 및 물 28.65g을 혼합하여 얻는다.Then, an additional 3% chitosan solution (experimental example: 30 g) was further added and stirred at 8,000 rpm for 10 minutes at high speed. 30 g of 3% chitosan solution is obtained by mixing 0.45 g of acetic acid, 0.9 g of chitosan and 28.65 g of water.

다음, 7% 구연산 용액(실험예: 10g)을 추가하고 10분 동안 8,000rpm으로 고속 교반한다. 7% 구연산 용액 10g은 구연산 0.7g에 물 9.3g을 넣어서 만든다.Next, 7% citric acid solution (experimental example: 10 g) was added and stirred at 8,000 rpm for 10 minutes at high speed. 10 g of 7% citric acid solution is prepared by adding 9.3 g of water to 0.7 g of citric acid.

이어서, 캐스터 오일(Caster Oil)(실험예: 1g)을 넣어 10분간 서서히 교반하여 캡슐레이션을 완료한다.
Then, castor oil (Experimental Example: 1 g) was added, and the mixture was gradually stirred for 10 minutes to complete the encapsulation.

이렇게 얻어진 저장성 향상 제제를 압력을 이용하여 과채류 포장용 부직포 등 포장 용지에 고르게 분사 후 건조한다.
The thus-obtained storage stability improving agent is uniformly sprayed on a packaging paper such as a nonwoven fabric for fruit and vegetable packaging using a pressure and dried.

이상에서 본 발명의 바람직한 실시예에 대하여 상세하게 설명하였지만 본 발명의 권리범위는 이에 한정되는 것은 아니고 다음의 청구범위에서 정의하고 있는 본 발명의 기본 개념을 이용한 당업자의 여러 변형 및 개량 형태 또한 본 발명의 권리범위에 속하는 것이다.While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.

Claims (5)

잣나무 오일 0.1%~10%, 알코올 20% 및 미량 첨가물을 포함하고, 제제의 총량이 100%가 되도록 증류수를 포함하는 잣나무 구과 추출물을 활용한 저장성 향상 제제.Improvement of storage stability using pine cone extract containing distilled water such that the pine tree oil contains 0.1% ~ 10%, alcohol 20% and trace additives, and the total amount of the preparation is 100%. 제1항에서,
상기 알코올은 폴리비닐알코올이고, 상기 미량 첨가물은 유화제, 키토산, 구연산, 캐스터 오일을 포함하는 잣나무 구과 추출물을 활용한 저장성 향상 제제.The method of claim 1,
Wherein the alcohol is polyvinyl alcohol, and the trace additive is a preservative improving agent using an extract of Pine wood comprising an emulsifier, chitosan, citric acid and castor oil. (가) 잣나무 오일을 유화제 및 알코올과 혼합하는 단계,
(나) 키토산 용액을 추가로 투입하고 교반하는 단계,
(다) 구연산 용액을 추가하고 고속 교반하는 단계,
(라) 캐스터 오일을 넣고 교반하여 캡슐레이션하는 단계
를 포함하는 잣나무 구과 추출물을 활용한 저장성 향상 제제 제조 방법.(A) mixing pine tree oil with emulsifier and alcohol,
(B) further adding and stirring a chitosan solution,
(C) adding a citric acid solution and stirring at a high speed,
(D) castor oil is added and stirred to encapsulate
And a method for manufacturing a preservation improving agent using the pine cone extract. 제3항에서,
상기 (가), (나), (다), (라) 단계에서 투입하는 잣나무 오일, 유화제, 알코올, 키토산, 구연산, 캐스터 오일의 질량비는 10 : 0.5 : 1 : 0.9 : 0.7 : 1인 잣나무 구과 추출물을 활용한 저장성 향상 제제 제조 방법.4. The method of claim 3,
The mass ratio of pine tree oil, emulsifier, alcohol, chitosan, citric acid and castor oil added in steps (a), (b), (c) and (d) is 10: 0.5: 1: 0.9: 0.7: A method for preparing a preservative-improving agent using an extract. 잣나무 오일 0.1%~10%, 알코올 20% 및 미량 첨가물을 포함하고, 제제의 총량이 100%가 되도록 증류수를 포함하는 잣나무 구과 추출물을 활용한 저장성 향상 제제를 함유하는 포장용지.A packaging paper containing a preservative improving agent using pine cone extract containing distilled water so that the pine tree oil contains 0.1% to 10%, alcohol 20% and trace additives, and the total amount of the preparation is 100%.
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