KR20160057532A - Cosmetic composition and food composition including hydrolysate product from silkworm - Google Patents

Abstract

The present invention relates to a cosmetic composition and a food composition containing a hydrolysate product from silkworm. A cosmetic composition and a food composition of the present invention are prepared using a method comprising: a first step for preparing silkworms; a second step for pulverizing the prepared silkworms to produce pulverized silkworms; a third step for adding acid to the pulverized silkworm and then hydrolyzing to produce a silkworm lysate; a fourth step for filtering the lysate to produce a filtrate, and adding a base to neutralize the filtrate and thereby produce a neutralized solution; a fifth step for desalting the neutralized solution to obtain a hydrolysate; and a sixth step for formulating a cosmetic composition or a food composition containing the hydrolysate as an active ingredient. By using the hydrolysate product from silkworm, the present invention provides a cosmetic composition which is harmless to the human body and has a skin-whitening effect, and a food composition having an effect of treating joint diseases.

Description

(Cosmetic composition and food composition including hydrolyzate product from silkworm)

The present invention relates to a cosmetic composition and a food composition comprising a hydrolyzate derived from a silkworm, and more particularly to a cosmetic composition having a whitening effect and harmless to humans through a silkworm-derived hydrolyzate and a food composition .

The silkworm, which is called as a sleepwalker, is a larva of Bombyx mori (culture word: silkworm). The silkworm hatching in eggs for 10 to 14 days after the initiation of silkworms is called a silkworm or an anthropomorphic silkworm, It has many hairs, about 5 mm in size, similar to ants.

From 5th to 6th day after the silkworms became 5th, the silk was extracted from the silkworms to form a cocoon. After 12-13 days from the cocoon, the silk was produced by the silkworm industry.

The noueun pupa, a natural noodle gangjeong food in Dongbokgok, is called zukonja (蠶 踊 子) in one room, and it is said that it has a temperate, tasteless, poisonous, And there is also a drug efficacy that eliminates diabetes or worms.

Recently, the industrial market related to the silkworm in Korea has been actively developed from the traditional silkworm industry, silkworm, which is a silkworm, and related to functional food or functional cosmetics based on silkworm.

Related prior arts include Korea Patent No. 10-1414040 (registered on June 25, 2014, name: pharmaceutical composition for regulating blood sugar, active ingredient of natural silkworm extract and health functional food) and Korea Patent No. 10 -0542266 (registered on Jan. 03, 2006, name of cosmetic pack composition containing sericin protein extracted from silkworm cocoons).

As described above, silkworm cocoons or the sericin or fibroin protein constituting these silkworms have been used as main ingredients for cosmetics or functional foods, but there is no research result related to the improvement effect of articular diseases with the silkworm itself.

An object of the present invention is to provide a cosmetic composition comprising a silkworm-derived hydrolyzate which is harmless to the human body and also has a whitening effect, and a food composition comprising a silkworm-derived hydrolyzate having an improving effect on joint diseases.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the particular embodiments that are described. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not restrictive of the invention, There will be.

To achieve the above object, a cosmetic composition comprising a silkworm hydrolyzate according to the present invention comprises a first step of preparing a silkworm, a second step of grinding the prepared silkworm to produce a silkworm crushed material, A third step of hydrolysis to prepare a lysate, a step of filtering the lysate, adding a alkali to the filtered solution to prepare a neutralized liquid, and desalting the neutralized liquid to obtain a hydrolyzate And a sixth step of preparing a cosmetic composition containing the hydrolyzate as an active ingredient.

Another method for producing a food composition comprising a silkworm-derived hydrolyzate according to the present invention comprises a first step of preparing a silkworm, a second step of grinding the prepared silkworm to produce a silkworm crushed material, A third step of hydrolysis after addition to prepare a lysate, filtering the lysate, adding a alkali to the filtered solution, and neutralizing the lysate to prepare a neutralized liquid, and desalting the neutralized liquid to obtain a hydrolyzate And a sixth step of preparing a food composition containing the hydrolyzate as an active ingredient, thereby having an improving effect on joint diseases.

The present invention provides a cosmetic composition including a silkworm-derived hydrolyzate, which is harmless to the human body and has a whitening effect, and a food composition having an improvement effect on joint diseases.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a diagram showing the degree of decrease in molecular weight by a silkworm-derived hydrolyzate of the present invention. Fig.
1: Marker
2: silkworm-derived hemolymph of Comparative Example 1
3: The silkworm-derived hydrolyzate of Example 1
Fig. 2 is a graph showing the tyrosinase inhibitory activity of the silkworm-derived hydrolyzate of the present invention.

The terms, techniques, and the like described in this specification are used in the meaning commonly used in the technical field to which the present invention belongs, unless otherwise specified.

Hereinafter, the cosmetic composition or the method of producing the food composition containing the hydrolysates derived from the silkworm of the present invention will be described in detail as follows.

Step 1: Prepare silkworms

Silkworms are prepared from silk raw materials, and any kind of silkworms may be used in the present invention.

2. The second step; Production of pulverized silkworms

In this step, the prepared silkworm is pulverized to produce a silkworm pulverized product.

At this time, the crushed silkworm may be produced by pulverizing the silkworm as it is, or it may be prepared by drying and crushing the silkworm. The particles of the silkworm silk pulp are preferably 600 to 800 mesh so that the following hydrolysis can be performed well.

3. The third step; Melt manufacturing

In this step, an acid is added to the silkworm silk pulp, and then hydrolyzed to prepare a melt.

At this time, hydrochloric acid is preferably used as the acid. This is because the molecular weight of the crushed silkworm is made small due to hydrochloric acid.

The hydrolysis is preferably performed at 100 to 120 ° C and 0.1 to 0.5 kgf / cm ² for 2 to 4 hours. This is because when the temperature of the hydrolysis exceeds 120 ° C and the time deviates from the above range, there is a possibility that the component is changed due to excessive hydrolysis and thus it is not suitable for use as a cosmetic or food material. If the temperature is less than 100 ° C, hydrolysis is not performed well, and it is difficult to produce the desired composition of the present invention.

4. Step 4; Manufacture of neutralizing liquid

In this step, alkali is added to the solution filtered after filtering the melt, and the solution is added until the pH becomes about 7.0 to 7.5, thereby neutralizing the melt to prepare a neutralized solution.

At this time, sodium hydroxide is preferably used as the alkali, and other types of alkali may be used. However, when using other types of alkali, the following desalting process is difficult.

5. Step 5; Obtain hydrolyzate

In this step, the neutralized liquid is desalted to obtain a hydrolyzate.

Such desalting is carried out for the purpose of obtaining a pure water hydrolyzate by an operation excluding the salts contained in the sample. Desalting methods can be all used as a conventional desalting method. However, The molecular weight of the dialysis membrane used for gel filtration, ultrafiltration, dialysis or the like is preferably 5 K or less, preferably 3.5 K or less .

6. Step 6; Composition preparation step

1) Preparation of cosmetic composition

The silkworm hydrolyzate obtained from the silkworm obtained is harmless to human body and has excellent tyrosinase inhibitory activity, and thus can be used as a main material of a cosmetic composition having a whitening effect. Thus, in this step, a cosmetic composition containing the hydrolyzate as an active ingredient is prepared.

The hydrolyzate derived from the silkworm may be contained in an amount of 1.6 to 200 μg based on 1 ml of the cosmetic composition, and the rest may contain other cosmetically acceptable substances. This is because when the hydrolyzate derived from silkworm is contained in an amount of less than 1.6 占 퐂 based on 1 ml of the cosmetic composition, the tyrosinase inhibitory activity of the present invention is insufficient and the amount of the hydrolyzate of the silkworm composition The tyrosinase inhibitory activity is reduced and it is not suitable for use as a material for a whitening cosmetic composition.

The formulations of the cosmetic compositions thus prepared may be applied differently depending on the method and purpose of use.

Examples of the formulations include lotions, liquid preparations, emulsions, emulsions, suspensions, tablets, and capsules.

When used as cosmetics, it can be used as cosmetics such as foundation lotion, lipstick, mascara or makeup base such as soft lotion, milk lotion, nutrition cream, massage cream, essence, cleansing foam, cleansing water, pack or body oil And can be blended with components commonly used in external preparations for skin such as an aqueous component, an oily component, a surfactant, a moisturizing agent, a thickening agent, an antiseptic, a perfume pigment and the like.

2) Food composition manufacturing

The obtained silkworm hydrolyzate can be used as a main material of a food composition which is harmless to humans and has an improving effect on joint diseases. Thus, in this step, a food composition containing the hydrolyzate as an active ingredient is prepared.

At this time, the hydrolyzate may be used as it is or may be used together with components of other conventional food compositions, and may be appropriately used according to a conventional method.

The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). Generally, when the food composition is prepared, it is added in an amount of 0.01 to 10 parts by weight, preferably 0.05 to 1 part by weight, based on the raw material of the active ingredient.

However, in the case of long-term consumption intended for health or hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

Such a food composition may be contained in a health food for the purpose of improving joint disease, and the kind thereof is not particularly limited. Examples of the food to which the above substances can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include healthy foods in a conventional sense.

In addition to the above, the food composition of the present invention may further contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, Alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the food composition of the present invention may be contained as pulp for the production of natural fruit juice, fruit juice drink and vegetable drink. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The present invention will be described in more detail with reference to the following examples and experimental examples, but the scope of protection is not limited to the following examples and experimental examples.

< Example  1> The silkworm origin of the present invention Hydrolyzate  Produce

The hydrolyzate was hydrolyzed at a high temperature and a high pressure (110 ° C, 0.1 kgf / cm ² ) for 3 hours after mixing 1 kg of the silkworm with 1:25 (w / v) of hydrochloric acid.

The hydrolyzate thus produced was filtered and neutralized by adding 3N sodium hydroxide thereto, followed by desalting to produce a silkworm hydrolyzate.

&Lt; Comparative Example 1 > Production of silkworm-derived hemolymph

First, silkworm dried (1 kg) was treated with liquid nitrogen and lyophilized (at -50 ° C for 70 hours).

From the silkworm dried material, the common glands were removed using a ball mill (diameter of 1 to 5 cm), and the remaining hemorrhoids were recovered.

<Experimental Example 1> Confirmation of molecular weight by SDS - PAGE

1. Experimental Method

To investigate the pattern of hydrolysates derived from silkworms, electrophoresis was performed using SDS-PAGE as described below.

In other words, the silkworm-derived hydrolyzate of the present invention was centrifuged again to remove cells, and the syringe filter (0.2 μm syringe filter, Sartorius) was used for filtering. To determine the molecular weights of these fermentation broths, electrophoresis was performed according to the manufacturer's manual using NuPAGE Bis-Tris Mini gel (novex, USA). The electrophoresed gel was stained with Coomassie Blue R-250.

2. Experimental results

As a result of the above experiment, it was confirmed that the molecular weight of the hydrolyzate (3) of Example 1 of the present invention was lower than that of silkworm lymphoma (2) of Comparative Example 1, as shown in Fig.

In other words, the fact that the molecular weight is small means that the product penetrates into the skin when used as a cosmetic material, so that the hydrolyzate produced through the manufacturing process of the present invention is suitable for use as a cosmetic material. In addition, since the water absorption rate is high even when used as a food material, the hydrolyzate produced through the manufacturing process of the present invention is suitable for use as a food composition material.

<Experimental Example 2> Nutritional analysis of hydrolysates derived from silkworms of the present invention

1. Experimental Method

1) General composition analysis

① Moisture

2 to 5 g of a sample (Example 1, Comparative Example 1) was precisely weighed in an aluminum weighing bottle which had been preliminarily dried and determined to have a constant weight, and was precisely weighed at 135 ± 2 ° C for 2 hours or at 105 to 110 ° C using a drying oven And allowed to stand for 30 minutes in a desiccator. The weight loss was determined by weight loss.

Water (%) = weight before drying (weighing bottle + sample) - weight after drying (weighing bottle + sample) / sample weight × 100

② crude fat

2 to 3 g of the sample (Example 1, Comparative Example 1) 2, dried at 95 ~ 100 ℃ for 2 hours, put in a fat extraction device, and ether was poured. After heating for 8 hours at 80 ℃, the fat was extracted and the fat was recovered and the fat was dried at 95 ~ 100 ℃ for 3 hours After cooling for 40 minutes, the sample was weighed, and the percentage of the sample was determined by measuring the crude fat content.

③ crude protein

And quantified by automated analysis using a Kjeltec auto 1035/38 sampler system.

2) Analysis of inorganic components

The inorganic components (calcium, phosphorus, potassium, sodium, magnesium, iron) were analyzed by ICP emission spectrometry.

2. Experimental results

The results of the experiment are shown in Table 1 below.

Analysis item name Comparative Example 1 Example 1 General component moisture(%) 3.63 2.37 Crude protein (%) 54.23 53.89 Crude fat (%) 7.21 0.19 Inorganic component

Calcium (Ca) (%) 0.24 0.11 (P) (%) 1.27 0.97 Potassium (K) (%) 3.76 0.79 Sodium (Na) (%) 0.11 5.83 Magnesium (Mg) (%) 0.36 0.08 Iron (Fe) (mg / kg) 46.85 214.35

As shown in Table 1, the hydrolyzate prepared through the manufacturing process of the present invention shows much lower crude fat content and contains much higher content of iron (Fe).

Thus, it was found that the hydrolyzate of the present invention can be used as a material of a food composition effective for improving joint diseases. In addition, the content of crude fat is low and it can be easily used as a health supplement food.

<Experimental Example 3> Determination of the amino acid composition of the silkworm-derived hydrolyzate of the present invention

1. Experimental Method

The amino acid composition of the silkworm-derived hydrolyzate of the present invention was confirmed by ion chromatography.

Specifically, 50 to 300 mg (about 30 mg as a crude protein) of the sample (Example 1 and Comparative Example 2) was put into a syringe and 40 ml of 6N-HCl was added. After nitrogen gas was injected, the stopper was closed, And then transferred to a rotary evaporator to remove hydrochloric acid at 50 ° C. When the evaporation is complete, the solvent is washed with distilled water, transferred to an evaporation flask, and evaporated again, and evaporated to dryness.

Then, a sample dilution buffer (pH 2.2) or distilled water was added to the evaporation flask which was finally evaporated to dryness to dissolve the amino acid. 5B filter paper to make 50 ml and used in this experiment.

2. Experimental results

The results of the experiment are shown in Table 2 below.

Amino acid type (%) Comparative Example 1 Example 1 Aspartic acid (ASP) 4.914 5.686 Threonine (THR) 2.059 2.463 Serine (SER) 2.262 3.403 Glutamic acid (GLU) 6.576 6.813 Glycine (GLY) 2.575 4.381 Alanine (ALA) 2.468 4.498 Valine (VAL) 2.247 2.742 Isoleucine (Ile) 1.749 1.954 Leu 2.834 3.272 Tyrosine (Tyr) 2.095 3.045 Phenylalanine (Phe) 1.894 2.086 Lysine (Lys) 3.067 3.012 Histidine (His) 1.829 2.059 Arginine (Arg) 2.510 2.511 Proline (Pro) 1.810 2.215

As shown in Table 2, the hydrolyzate of Example 1 of the present invention showed higher contents of serine, glycine and alanine than those of Comparative Example 1.

In other words, serine promotes insulin production, lowers blood glucose, and strengthens tinnitus. Alanine has chronic fatigue suppression, liver function enhancement, cholesterol inhibition and alcohol detoxification. Glycine inhibits cholesterol, liver function, It has been reported to be useful for showing the effect. Thus, it can be seen that the hydrolyzate of Example 1 of the present invention can be used as a material of a food composition exhibiting the above-described functionality.

EXPERIMENTAL EXAMPLE 4 [0060] The silkworm-derived hydrolyzate of the present invention Identification of tyrosinase inhibitory activity

1. Experimental Method

The inhibitory activity of tyrosinase activity was measured to determine whether there was a whitening effect inhibiting the activity of tyrosinase involved in the early stage of melanin production. Specifically, tyrosinase activity measurement was performed by changing the method of Choi et al. (1998), adding 40 μl of a sample to a 96-well plate and incubating 8.3 mM L-DOPA (3,4-Dihydroxy-L-phenylalanine D9628-5G , 120 μl of Sigma-Aldrich Co.) and 40 μl of 125 U (unit) tyrosinase from mushroom T3824 (Sigma-Aldrich Co.) were mixed. After incubation at 37 ° C for 30 minutes, the absorbance at 490 nm was measured and the amount of dopaquinone produced was calculated.

In order to measure tyrosinase activity in cells, melanocyte-producing cells (Melanocyte B16F1) were cultured in a 24-well plate at 100,000 cells / well for one day. After the medium was removed, the sample was mixed with the medium and incubated at 37 ° C, CO ₂ Lt; / RTI &gt; for 24 hours. Cells were collected with Trypsin-EDTA, centrifuged at 12,000 rpm for 5 minutes, and then the supernatant was removed. The precipitate was dissolved in protein extraction solution (NP-40) and then left in ice for 10 minutes. And centrifuged. The pellet was dissolved in 0.1 M sodium phosphate buffer (pH 6.8) supplemented with 0.2% L-DOPA and reacted at 37 ° C for 15 minutes. Absorbance was measured at 475 nm.

2. Experimental results

2, the tyrosinase inhibitory activity of the silkworm-derived hydrolyzate (AH) of Example 1 was confirmed to be more than twice as high as that of the silkworm lymphoma (HL) of Comparative Example 1. Thus, it can be seen that even when only a small amount of the hydrolyzate derived from silkworm of Example 1 of the present invention is used in the production of cosmetics, the economical efficiency is excellent. In addition, it is preferable that the hydrolyzate of the present invention is prepared so as to contain 1.6 to 200 占 퐂 of the cosmetic composition on the basis of 1 ml of the cosmetic composition when the cosmetic composition has a whitening effect.

Experimental Example 5: Confirmation of glucosamine content of the silkworm-derived hydrolyzate of the present invention

1. Experimental Method

1 ml of Schales reagent was added to 0.5 ml of the sample, incubated in boiling water for 15 minutes, cooled, and absorbance was measured at 420 nm.

2. Experimental results

The results of the experiment are shown in Table 3 below.

sample Glucosamine content (mg) Comparative Example 1 43.0 ± 1.37 Example 1 64.5 ± 1.24

As shown in Table 3, it was confirmed that the glucosamine content of the silkworm-derived hydrolyzate of Example 1 prepared by the method of the present invention was higher than that of the silkworm-derived blood lump of Comparative Example 1.

Thus, it was found that the hydrolyzate of the present invention can be used as a material of a food composition effective for improving joint diseases.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. You can implement the examples. The scope of the present invention is defined by the appended claims, and all differences within the scope of the claims are to be construed as being included in the present invention.

Claims (12)

A first step of preparing a silkworm;
A second step of pulverizing the prepared silkworm to produce a silkworm pulp;
A third step of adding an acid to the crushed silkworm and hydrolyzing the silkworm to produce a melt;
A fourth step of filtering the lysate, filtering the lysed solution, adding alkali to the lysed solution, and neutralizing the solution to prepare a neutralized solution;
A fifth step of desalting the neutralized liquid to obtain a hydrolyzate,
And a sixth step of preparing a cosmetic composition containing the hydrolyzate as an active ingredient.
A method for producing a cosmetic composition comprising a silkworm-derived hydrolyzate.
The method according to claim 1,
Wherein the acid is hydrochloric acid in the third step,
A method for producing a cosmetic composition comprising a silkworm-derived hydrolyzate.
The method according to claim 1,
Wherein the hydrolysis is performed at 100 to 120 ° C and 0.1 to 0.5 kgf / cm ² for 2 to 5 hours in the third step.
A method for producing a cosmetic composition comprising a silkworm-derived hydrolyzate.
The method according to claim 1,
Wherein said alkali is sodium hydroxide in said fourth step,
A method for producing a cosmetic composition comprising a silkworm-derived hydrolyzate.
The method according to claim 1,
Wherein the hydrolyzate is contained in an amount of 1.6 to 200 占 퐂 based on 1 ml of the cosmetic composition in the sixth step.
A method for producing a cosmetic composition comprising a silkworm-derived hydrolyzate.
Which is produced by a process according to any one of claims 1 to 5,
Which has a whitening effect through tyrosinase inhibitory activity,
A cosmetic composition comprising a silkworm-derived hydrolyzate.
The cosmetic composition according to claim 6, characterized in that it has a formulation of any one of lotion, essence, lotion, cream, pack, foundation, gel, ointment or spray.
A cosmetic composition comprising a silkworm-derived hydrolyzate.
A first step of preparing a silkworm;
A second step of pulverizing the prepared silkworm to produce a silkworm pulp;
A third step of adding an acid to the crushed silkworm and hydrolyzing the silkworm to produce a melt;
A fourth step of filtering the lysate, filtering the lysed solution, adding alkali to the lysed solution, and neutralizing the solution to prepare a neutralized solution;
A fifth step of desalting the neutralized liquid to obtain a hydrolyzate,
And a sixth step of preparing a food composition containing the hydrolyzate as an active ingredient.
A method for producing a food composition comprising a silkworm-derived hydrolyzate.
The method of claim 8,
Wherein the acid is hydrochloric acid in the third step,
A method for producing a food composition comprising a silkworm-derived hydrolyzate.
The method of claim 8,
Wherein the hydrolysis is performed at 100 to 120 ° C and 0.1 to 0.5 kgf / cm ² for 2 to 5 hours in the third step.
A method for producing a food composition comprising a silkworm-derived hydrolyzate.
The method of claim 8,
Wherein said alkali is sodium hydroxide in said fourth step,
A method for producing a food composition comprising a silkworm-derived hydrolyzate.
A process for the preparation of a compound according to any one of claims 8 to 11,
A characteristic feature of improving the joint disease,
A food composition comprising a silkworm-derived hydrolyzate.

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