KR20160057137A - Cosmetic Composition with Anti-Temperature Stress Comprising Rhodiola Sachalinensis Extract As Active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a Rhodiola sachalinensis extract as an active component for minimizing death of skin cells caused by high temperature or low temperature stress, thereby relieving skin damages.

Description

[0001] The present invention relates to a cosmetic composition for alleviating skin temperature stress comprising an extract of Rhodiola griseus as an active ingredient,

The present invention relates to a cosmetic composition containing an extract of Rhodiola grisea as an active ingredient and having an effect of alleviating skin damage by minimizing skin cell death due to high temperature or low temperature stress.

The skin is the largest organ of the human body that protects various organs from the outside to the inside of the skin. It has barrier function to prevent the internal moisture and oil components from flowing out of the skin, temperature control function, excretion function, breathing Function, and so on.

However, skin can cause problems due to physiological factors, nutritional factors, external environmental factors, psychological factors, biochemical factors, life factors, and medical factors.

Especially, when the temperature of the skin is increased by ultraviolet rays or infrared rays, various enzymes such as substrate proteases in the skin are affected, and thus, symptoms such as skin redness, skin dryness, and the like may occur have. In addition, due to recent changes in the living environment, the use of steam rooms and saunas has caused many people to expose their skin to high temperatures.

In addition, sudden temperature drop due to fall in winter temperature and long-term exposure to low temperature conditions can cause environmental stress to the skin. During the winter season, when the senses of the skin fall, frostbite, outdoor activities, or going out and returning, you can get burned at low temperatures.

Therefore, there is a demand for development of a cosmetic composition capable of inhibiting skin cell damage due to such high temperature or low temperature.

Accordingly, the present inventors have made various efforts to develop a cosmetic composition having an effect of mitigating skin damage due to temperature stress while containing a natural extract as an active ingredient. As a result, in the case of the Hongkong stream extract, not only high temperature but also skin cell death Can be minimized, thereby completing the present invention.

Accordingly, it is an object of the present invention to provide a cosmetic composition containing as an active ingredient, a natural extract capable of minimizing skin cell death due to high-temperature or low-temperature stress and mitigating skin damage.

To this end, the present invention provides a cosmetic composition for alleviating skin temperature stress comprising a Rhodiola extract as an active ingredient.

The cosmetic composition containing the extract of the present invention as an active ingredient has the effect of alleviating skin damage due to high temperature or low temperature stress, and thus it can secure high value as a cosmetic ingredient.

FIG. 1 is a graph showing cell numbers after cold stressed fibroblasts pretreated with Rhodiola extract.
FIG. 2 is a graph comparing the number of cells subjected to high temperature stress to the fibroblasts pretreated with the extract of Hongkong Stream.

Hereinafter, the present invention will be described in more detail.

In the present invention, a cosmetic composition containing as an active ingredient an extract of Hong Kyung Chun having an effect of alleviating skin damage due to temperature stress as an external environmental factor is presented.

Rhodiola Sachalinensis A. Bor is a perennial herbaceous plant inhabiting a high mountainous region about 2000 to 5000 m above sea level. Hongkongcheon has a special adaptability that can survive in the environment of a high mountain where the diagonal difference is large and dry, oxygen is lean, and ultraviolet rays are strong, and is also called alias such as alpine gyecho stream or alpine stream gyeongche stream. The major components of Hong Kyung Chun include salicyloids, tyrosol, and glycosides as well as starch, proteins, fats, sugars, flavones, amino acids, inorganic elements, and minor essential oils.

In the present invention, the Rhodiola extract is obtained by extracting from various organs or parts, and is characterized in that a solvent is added to at least one selected from rhizome, root, and shoot of Rhodiola.

The Rhodiola extract used as a raw material in the present invention is contained in an extract obtained by leaching and transferring from Hongikong Stream or a concentrate obtained by partially or entirely concentrating the leached liquid or an extract and an extract prepared by drying the concentrate again And the chemicals themselves that exert the main effect.

The extract of the present invention can be produced by a conventional method known in the art, that is, by using a conventional solvent, such as a commonly used method such as cold-water treatment, reflux cooling, hot water extraction, room temperature extraction, warm- Extraction, and the like. Preferably, it is refluxed, cooled, and filtered.

Specific examples thereof include purified water, alcohols having 1 to 6 carbon atoms, glycerin, alkylene glycols having 2 to 6 carbon atoms, alkanes having 1 to 6 carbon atoms, halogenated hydrocarbons having 1 to 6 carbon atoms, alkylalkanoates having 2 to 6 carbon atoms, and mixtures thereof Can be used. More specifically, extraction solvents selected from purified water, methanol, ethanol, glycerin, ethylene glycol, butylene glycol, propylene glycol, dichloromethane, dichloroethane, hexane, ethyl acetate and mixtures thereof can be used.

In this case, the amount of the extraction solvent used is 10 to 30 times the volume of the raw material, more preferably 10 to 20 times.

The extraction temperature can be varied by a person skilled in the art in a variety of temperature ranges suitable for the extraction method, for example, but not limited to, from 20 캜 to 100 캜. In addition, the extraction time differs depending on the extraction method, and a person skilled in the art can adopt an appropriate extraction time, and can be performed in a single or multiple cycles in a range of about 1 hour to several days, though it is not limited thereto. The extract obtained by performing the extraction with the primary extraction solvent may be obtained in the form of a liquid in which impurities are removed by filtration according to a conventional method, or the obtained liquid form extract may be concentrated under reduced pressure and / Can be obtained.

The content of the Rhodiola extract according to the present invention is preferably in the range of 0.001 to 30.0% by weight based on the whole composition. The content of the extract is preferably at least the above-mentioned minimum value so as to attain the minimum skin temperature stress relieving effect, and the content of the extract is preferably less than the maximum value in consideration of the deterioration of use feeling due to the excessive addition and the applicability to various formulations. At this time, it is preferable that the content of the extract is appropriately controlled within the above range according to the content of the components contained in the formulation.

In addition, when the Rhodiola extract according to the present invention is used as a constituent component, it can be used not only in the form of a lyophilized powder, but also in a liquid form dispersed or dissolved in water or a common organic solvent.

In addition, the cosmetic composition according to the present invention may contain, in addition to the above-mentioned extract components, other components which can give synergistic effects to the main effect, within the range not impairing the intended main effect of the present invention.

The ingredients contained in the cosmetic composition of the present invention contain, in addition to the above-mentioned effective ingredients, the ingredients commonly used in cosmetic compositions and may contain conventional additives such as stabilizers, solubilizers, vitamins, pigments and fragrances, have.

The cosmetic composition of the present invention can be prepared in any form conventionally produced in the art and specifically includes a solution, a suspension, an emulsion, a gel, a lotion, an essence, a cream, a powder, a soap, a pack mask, , Foundation, spray, and the like.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Hydroxides, microcrystalline cellulose, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a cream or a gel, wax, paraffin, trachant, animal oil, starch, cellulose derivative, silicone, bentonite, polyethylene glycol, silica, zinc oxide or talc may be used as the carrier component.

When the formulation of the present invention is a powder or a spray, the carrier component may include a propellant such as silica, talc, aluminum hydroxide, lactose, calcium silicate, chlorofluorohydrocarbons, propane / butane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, it is preferable to use, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, imidazolinium derivative, isethionate, methyltaurate, sarcosinate, fatty acid amide ether Aliphatic alcohols, alkylamidobetaines, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided to further understand the present invention, and the present invention is not limited by the examples.

Example  One:  Hong Kyung Chun Extract

After 200 g of the root of Hongikyecheon was grinded and pulverized, 1.5 l of water was added and the mixture was extracted at 100 ° C for 3 hours and concentrated under reduced pressure with a rotary evaporator (EYELA Digital water bath SB-1000, Japan).

After the extraction, the filtrate was filtered to obtain a filtrate. Water was added to adjust the total volume to 100 ml. The adjusted solution was left to stand at 4 ° C for 6 days. The resulting precipitates were filtered through 0.45 μm membrane to obtain a Rhodiola extract.

Experimental Example  1: Fiber due to low temperature stress Asbestos Proliferative ability  Measure

1-1. Cultivation of human skin fibroblasts

Human dermal fibroblast was purchased from Gibco (Life Technologies, Carlsbad, Calif., USA). The cells were cultured in DMEM medium (Gibco) at 37 ° C. The medium was maintained until the adhered cells were confluent at 80% and subcultured at 80% confluence. The medium was replaced every two days until confluence. For the subculture, the flask in which the culture solution had been removed was washed with phosphate buffered saline (PBS), and the cells were suspended in 0.25% trypsin-EDTA (Gibco BRL, Grand Island, NY, USA) After that, the number of cells was measured and subcultured again in the culture solution three times.

1-2. Measurement of proliferation by cold stress

DMEM medium (Gibco) supplemented with 10% serum in a 60-mm culture dish was inoculated with 5 x 10 ⁵ human fibroblasts and cultured at 5% CO ₂ and 37 ° C, And then cultured in serum-free culture medium for 24 hours. Then, the cells were divided into negative control group, positive control group, and Hongkryungchun extract treatment group. The negative control group was not treated at low temperature and the positive control group was treated at low temperature. In the group treated with Rhodiola extract, 10 μl of the extract prepared in Example 1 was dissolved in distilled water at a concentration of 0.05 to 1% by weight.

After the treatment, the cells were cultured at 5% CO ₂ and 37 ° C for 24 hours. After completion of the incubation, the cells were washed with phosphate buffer saline (PBS) and incubated at 4 ° C for 19 hours using a low-temperature incubator. After 4 hours, MTT reagent was added and the medium was removed. 200 μl of DMSO was added to dissolve the formazan crystal, and the absorbance was measured at 570 nm with a microplate reader. The results are shown in Fig.

From the results shown in FIG. 1, it can be seen that the cell proliferation is increased in the treated condition of the present invention when compared with the cell death of the fibroblast according to the cold stress based on the control group.

Experimental Example  2: Fiber due to high temperature stress Asbestos Proliferative ability  Measure

2-1. Measurement of proliferation by high temperature stress

DMEM medium (Gibco) supplemented with 10% serum in a 60-mm culture dish was inoculated with 5 x 10 ⁵ human fibroblasts and cultured at 5% CO ₂ and 37 ° C, And then cultured in serum-free culture medium for 24 hours. Then, the cells were divided into negative control group, positive control group, and Hongkryungchun extract treatment group. The negative control group was not treated at high temperature and the positive control group was treated at high temperature. In the group treated with Rhodiola extract, 10 μl of the extract prepared in Example 1 was dissolved in distilled water at a concentration of 0.05 to 1% by weight.

After the treatment, the cells were cultured at 5% CO ₂ and 37 ° C for 24 hours. After completion of the incubation, the cells were washed with phosphate buffer saline (PBS) and incubated at 48 ° C for 40 minutes using a high-temperature incubator. After 4 hours, MTT reagent was added and the medium was removed. 200 μl of DMSO was added to dissolve the formazan crystal, and the absorbance was measured at 570 nm with a microplate reader. The results are shown in Fig.

From the results shown in FIG. 2, it can be seen that the cell proliferation is increased in the treated condition of the present invention when compared with the cell death of the fibroblast according to the high temperature stress when the control group is used as a reference.

Hereinafter, a preferred formulation example is provided to facilitate understanding of the present invention. However, the following formulation examples are provided only for a better understanding of the present invention, and the present invention is not limited by the formulation examples.

Formulation Example 1: Softening Longevity (Skin)

The number of softening times containing the extract of Example 1 was prepared according to a conventional method as shown in Table 1 below.

ingredient Content (% by weight) The extract of Example 1 1.0 glycerin 5.0 1,3-butylene glycol 3.0 Betaine 1.0 Allantoin 0.1 EDTA-2Na 0.02 Sodium hyaruronate powder 0.05 ethanol 5.0 Octyldodec-16 0.2 Polyoxyethylene hardened castor oil 0.2 antiseptic Suitable amount Purified water Balance Sum 100

Formulation Example 2: Nourishing lotion (lotion)

As shown in the following Table 2, a nutritional lotion containing the extract of Example 1 was prepared according to a conventional method.

ingredient Content (% by weight) The extract of Example 1 2.0 Glyceryl stearate SE 1.5 Cetearyl alcohol 1.0 Sheer butter 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil 1.0 Mineral oil 5.0 Squalane 3.0 Cyclomethicone 2.0 Dimethicone 0.8 Tocopheryl acetate 0.5 Carbomer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium hyaruronate powder 0.05 Triethanolamine 0.12 antiseptic Suitable amount Purified water Balance Sum 100

Formulation Example 3: Massage cream

A massage cream containing the extract of Example 1 was prepared according to a conventional method as shown in Table 3 below.

ingredient Content (% by weight) The extract of Example 1 2.0 Glycerin monostearate 1.5 Cetearyl alcohol 1.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl isoserate 5.0 Squalane 5.0 Mineral oil 35.0 Dimethicone 0.5 Hydroxyethylcellulose 0.12 glycerin 6.0 1,3-butylene glycol 3.0 Triethanolamine 0.3 antiseptic Suitable amount Purified water Balance Sum 100

Formulation Example 4: Essence

Essences containing the extract of Example 1 were prepared according to a conventional method as shown in Table 4 below.

ingredient Content (% by weight) The extract of Example 1 2.0 glycerin 6.0 Betaine 5.0 PEG 1500 2.0 Allantoin 0.1 EDTA-2Na 0.02 Hydrogenated lecithin 0.6 Hydroxyethylcellulose 0.1 Sodium hyaruronate powder 0.08 Carboxyvinyl polymer 0.2 Triethanolamine 0.2 Ceramide 0.2 Octyldodecanol 3.0 Squalane 3.0 Polysorbate 60 0.4 Glyceryl stearate SE 1.5 antiseptic Suitable amount Purified water Balance Sum 100

Formulation Example 5: Pack

A pack containing the extract of Example 1 was prepared according to a conventional method as shown in Table 5 below.

ingredient Content (% by weight) The extract of Example 1 1.0 Polyvinyl alcohol 15.0 Cellulose sword 0.15 glycerin 3.0 PEG 1500 2.0 Betaine 2.0 Allantoin 0.1 Triethanolamine 0.2 Nicotinamide 0.5 ethanol 6.0 PEG 40 hardened castor oil 0.3 antiseptic Suitable amount Purified water Balance Sum 100

Claims (8)

A cosmetic composition for alleviating skin temperature stress containing Rhodiola Sachalinensis extract as an active ingredient. The method of claim 1, wherein the extract
A cosmetic composition for skin temperature stress relief characterized by containing 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. The method of claim 1, wherein the extract
A cosmetic composition for relieving skin temperature stress, characterized by having a liquid phase, a concentrated solid phase and a lyophilized powder phase. The method according to claim 1,
A cosmetic composition for skin temperature stress relief characterized by being extracted with an extraction solvent selected from purified water, methanol, ethanol, glycerin, ethylene glycol, propylene glycol, butylene glycol, hexane, dichloromethane, dichloroethane, ethyl acetate and mixtures thereof . The method of claim 1, wherein the extract
A cosmetic composition for relieving skin temperature stress, characterized in that it is extracted from cold extraction, ultrasonic extraction, reflux cooling extraction, hot water extraction, room temperature extraction, warming extraction and supercritical extraction. The method of claim 1, wherein the extract
A cosmetic composition for skin temperature stress relief characterized by having a low-temperature stress relieving effect. The method of claim 1, wherein the extract
A cosmetic composition for skin temperature stress relief characterized by having a high temperature stress relieving effect. 8. The cosmetic composition according to any one of claims 1 to 7,
A cosmetic composition for skin temperature stress relief having one type of formulation selected from the group consisting of a solution, a suspension, an emulsion, a gel, a lotion, an essence, a cream, a powder, a soap, a pack mask, a cleansing oil, a foundation and a spray.

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