KR20160033283A - Preparation Method of Exreacts of Sea Slug with High Yield Using Enzyme - Google Patents

Abstract

Provided in the present invention is a manufacturing method of a sea cucumber extract, which comprises the steps of: (1) adding protamex to sea cucumber powder and enzymatically degrading the same; (2) filtering the enzymatically degraded product obtained from step (1); and (3) freeze-drying a filtrate obtained from step (2). According to the present invention, the sea cucumber extract with abundant bioactive materials at high yields can be obtained through a simple and inexpensive method.

Description

[0001] The present invention relates to a method for preparing a high yielding sea cucumber extract using an enzyme,

The present invention relates to a method for producing a sea cucumber extract, and more particularly, to a method for producing a sea cucumber extract having a high yield, in which a sea cucumber extract rich in physiologically active substances can be obtained at a high yield through a simple and inexpensive method.

Sea cucumber is a medicinal herb that has been found in China and other Eastern countries for a long time. It has excellent effects on diabetes and asthma. It is a medicine that can regain energy when it is swollen due to temperature increase in summer It is known. When a part of the body is cut, the sea cucumber is restored to its original state within 3 months, and even when the intestines are removed, the sea cucumber has remarkable resilience that a new intestine is formed within one month. In the oriental medicine and folk therapy, It is known that it enhances immune function and improves wound healing.

According to modern science, sea cucumbers are abundant in protein and vitamin B, contain a large amount of iodine, and contain a large amount of chondroitin, which is used as a raw material for various foods and medicines.

The major components of sea cucumber are glycoprotein and chondroitin sulfate, and glycoproteins are mainly composed of polysaccharides such as fucose, glucose, mannuronic acid and N-acetylglucosamine.

Nutritional studies on sea cucumbers include studies on acidic mucopolysaccarides, which are the main factors for platelet aggregation, and studies on the structural properties of sulfated polysaccharides with structures such as chondroitin sulfate (Vieira et al., J Biol. Chem., 263, 18176, 1998) and the contents and contents of glucosaminoglycans isolated from the body wall of sea cucumber (Kariya et al., J. Biol. Chem., 265, 5081 , 1990) and revealed the structure of chondroitin sulfate extracted from sea cucumber walls (Vieira et al., J. Biol. Chem., 266, 13530, 1991).

Thus, the sea cucumber composed mainly of sticky glycoprotein can be easily degraded through contact with oxygen during circulation. To overcome such disadvantages, the sea cucumber is usually dried .

However, it is true that dried ginseng itself has high nutritional and physiologically active ingredients and thus has a high value in food science. However, since it can not be provided as an additive for other processed foods, there is a limit to increase the added value. It is required to be processed. Although the hot water extraction method is mainly known as a process for producing an extract using sea cucumbers, the extraction yield is not so high according to this method, and the non-economical efficiency of requiring a large amount of sea cucumber is a problem.

SUMMARY OF THE INVENTION Accordingly, the present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a method for preparing a sea cucumber extract having a high yield by a simple and inexpensive method at a high yield of a sea cucumber extract rich in physiologically active substances. .

The technical problem of the present invention as described above is achieved by the following means.

(1) 1) adding protamex to the sea cucumber powder to extract the enzyme;

2) filtering the enzyme extract obtained in the step 1); And

3) freeze-drying the filtrate obtained in the step 2);

≪ / RTI >

(2) In the above (1)

The sea cucumber powder is prepared by first removing raw sea cucumbers and then matured.

Cryopreservation of the first matured sea cucumber;

Secondly dipping the cryo-cryopreserved frozen; And

Drying and crushing the second matured sea cucumber;

The method of claim 1,

(3) In the step (2), drying and pulverizing the second-

Dried sea cucumber is dried in a dry oven, and then the expanded sea cucumber is expanded at 10 to 13 kg / cm 2 and at 110 to 130 ° C for 2 to 10 minutes using a puffing device, and then pulverized using a blender ≪ / RTI >

(4) In the above (1)

Wherein the enzyme decomposition of step (1) is carried out at 35 to 45 ° C for 5 to 10 hours.

(5) In the above (2)

Wherein the sea cucumber powder is passed through a 2 to 3 mm mesh.

(6) In the above (1)

The enzyme is mixed and compounded at a ratio of 1: 0.5: 1 to 1: 1: 1: 0.5: 1 to 1: 1, or ProTamex: Papain (or ProTex) Wherein the method comprises the steps of:

(7) In the above (1)

Wherein the enzyme is treated with a low frequency of 1 to 10 kHz for 6 to 7 hours.

As described above, according to the present invention, it is possible to obtain the extract of sea cucumbers rich in physiologically active substances through a simple and inexpensive method at a high yield, thereby contributing to the promotion of income of the sea cucumber farmers as well as promotion of national health, Exports are expected to contribute greatly to the growth of the national economy.

1 is a process flow diagram of a sea cucumber extract according to the present invention.

In order to accomplish the above object, the present invention provides a method for preparing an extract of sea cucumber,

(1) adding protease to the sea cucumber powder to perform enzymatic degradation;

(2) filtering the enzyme hydrolyzate obtained in the step (1); And

(3) freeze-drying the filtrate obtained in the step (2).

Hereinafter, the contents of the present invention will be described in detail with reference to FIG.

In the present invention, the sea cucumber powder is used to obtain an extract of sea cucumber rich in physiologically active substances at a high yield through a simple and inexpensive method.

The sea cucumber powder is preferably prepared by first removing the viscera from the sea cucumber and then ripening it first; Cryopreservation of the first matured sea cucumber; Secondly dipping the cryo-cryopreserved frozen; And a step of drying and pulverizing the second-matured sea cucumber.

The first masturbation process includes a process in which 3 to 5 times of water is added to the weight of sea cucumber using the sea cucumbers from which the viscera is removed as a raw material.

The first mating process is preferably carried out at 90 to 100 ° C. If the matured material is less than 90 ° C, it is difficult to obtain sufficient matured material. If the temperature exceeds 100 ° C, there is a fear of elution or destruction of various physiologically active substances high.

In this case, the first cooking time is preferably 10 to 60 minutes. If the time is exceeded, various physiologically active substances or functional oil components of sea cucumber may be destroyed.

The cryopreservation of the first matured sea cucumber is carried out at -20 to -18 캜, which is effective not only to improve storage stability but also to be able to be taken out and used as needed. In order to increase the yield of the extract, It is also required for the process. For this purpose, cryopreservation is preferably carried out for 1 to 2 days.

The first matured sea cucumber which has been frozen and stored is used as a raw material and the second matured process is performed by adding 3 to 5 times water to the weight of the sea cucumber as in the first matured process.

The second matured process is preferably carried out at 90 to 100 ° C. If the matured material is less than 90 ° C., it is difficult to sufficiently obtain the matured material. If the temperature exceeds 100 ° C., there is a fear of elution or destruction of various physiologically active materials high.

At this time, the second cooking time is preferably 10 to 60 minutes as in the first cooking process. If the time is exceeded, various physiologically active substances or functional oil components of sea cucumber may be destroyed.

The second mature sea cucumber prepared through the above process is dried in a dry oven, and then is ground as a raw material by using a blender or the like. At this time, pulverization using a blender is preferably carried out in a size having a diameter of about 2 to 3 mm in order to maximize the yield of the extract.

More preferably, the second mature sea cucumber is dried in a dry oven, and then the expanded sea cucumber is expanded at 10 to 13 kg / cm 2 and at 110 to 130 ° C for 2 to 10 minutes using a puffing device, followed by pulverization using a blender, It is good for maximizing the extraction yield by using as raw material.

The proteasma enzyme protease is added to the prepared sea cucumber powder, and the enzyme reaction is performed by adding protamex. At this time, it is preferable that the prothamex is added in an amount of 0.01 to 10 parts by weight based on 100 parts by weight of the sea cucumber powder.

The extraction process of protease by protease generally improves the yield of the extract greatly compared to the process of hydrothermal extraction or organic solvent extraction, and the yield of the extract can be greatly increased even when other proteases are used alone .

The enzymatic extraction by protease is preferably carried out at 35 to 45 ° C, more preferably at 40 ° C for 5 to 10 hours, more preferably 6 to 7 hours. Under such conditions, the yield of the extract is maximized.

In the present invention, in order to further enhance the extraction yield using the enzyme, it is preferable to treat the enzyme for 6 to 7 hours using a low frequency of 1 to 10 kHz.

More preferably, it is preferable to use papain or protex or papain and protex in a predetermined ratio, rather than using protamex alone, in terms of yield improvement. Preferably 1: 0.5 to 0.5: 1 to 1: 1: 1, in terms of the weight ratio of protamine: papain (or protex) = 1: 0.5 to 1: 1 or protamax: papain: I will give you.

After the enzymatic extraction of the powdery sea cucumber as described above, the obtained enzyme extract is collected and filtered under reduced pressure. The vacuum filtration process may preferably be performed using equipment equipped with 3M paper or any other vacuum filtration device commonly used.

For example, one of the pressure filtration method using a vacuum pump can be continuously carried out while maintaining the degree of vacuum of not more than 1 atmospheric pressure, of 45 ~ 55kgf / cm ² between 10 and 14 liters per minute at a pressure of using the high-pressure pump The enzyme extract may be supplied to the reverse osmosis membrane to remove the salt, and the salt-removed enzyme extract may generate 1 to 3 liters per minute.

The filtrate obtained through the vacuum filtration process as described above is dried through a freeze-drying process. The lyophilizate is obtained by sublimation, which freezes the extract material and lowers the partial pressure of water vapor, thereby directly directing the ice to steam. Here, lowering the partial pressure means lowering the pressure to below the triple point of water (6 mbar or 4.6 Torr), and the water in the form of ice under low pressure is not converted into liquid by supplying heat energy, Sublimes. Since the sublimed ice crystals leave space, the dried material contains a large number of gaps, so that the water absorption is easy and provides the property of completely re-hydrating upon re-hydration.

Through the above process, the extract of the sea cucumber rich in physiologically active substances can be obtained at a high yield through a simple and inexpensive method.

Hereinafter, the present invention will be described in more detail with reference to examples. However, these embodiments are for the purpose of understanding the present invention and should not be construed as limiting the scope of the present invention.

[Example 1] Preparation of sea cucumber powder

As shown in FIG. 1, the raw sea cucumbers were first purchased, the viscera were removed, and the first mashing process was performed by boiling at 100 ° C for 30 minutes. The above-mentioned first-matured sea cucumber was stored frozen at -20 ° C for 1 day, and further boiled at 100 ° C for 30 minutes to carry out a second cooking process.

Secondary mature sea cucumbers prepared as described above were placed in a dry oven, dried, ground by a blender, and sieved with a mesh to obtain 2 to 3 mm diameter powder.

[Example 2] Production of expanded sea cucumber powder

The second matured sea cucumber was dried in a dry oven, and then the expanded sea cucumber obtained by expanding at 12 kg / cm 2 at 130 ° C for 10 minutes using a puffing device was pulverized using a blender. Sea cucumber powder was prepared.

[Example 3] Optimal enzyme and sample selection

2 g each of the mature sample, the dry sample and the powder sample of Example 1 and the expanded powder sample of Example 2 were each taken in four 50 ml conical tubes. 20 μl of Protex, Papain and Protamex as protease were added to each sample together with 30 ml of distilled water.

The control group to which Protex and Papain were added singly was allowed to react for 6 hours in a water bath at 65 ° C. and the Protamax-added experimental group for the present invention was allowed to react in a water bath at 40 ° C. for 6 hours.

As a result of the enzymatic reaction as described above, when the sample was observed with the naked eye, proteamase was used as the main enzyme and the protease was selected as the main enzyme.

After the enzymatic reaction on the powdered sea cucumber as described above, the obtained protamex extract was collected and filtered under reduced pressure. Vacuum filtration was performed by washing with distilled water using 3M paper (see Table 1). At this time, the unfiltered suspension was dried and weighed to calculate the amount of enzyme reaction.

Example 1
(Sample of matured) Example 1
(Dry sample) Example 1
(Powder sample) Example 2
(Powder sample) Amount of unfiltered residue 0.72 g 1.03 g 0.24 g 0.15 g Enzyme resolution 64.0% 48.5% 88.0% 92.5%

As can be seen from the results in Table 1, the enzyme reaction of the powder samples of Examples 1 and 2 was the best.

[Example 4] Preparation of sea cucumber extract using protamex

30 g of the sample of the sea cucumber powder obtained in Example 1 was placed in a 1000 ml flask, 300 mg of Protamex and 450 ml of distilled water were added thereto, and the mixture was reacted in a water bath at 40 ° C for 6 hours. After completion of the enzyme reaction, the filtrate was subjected to reduced pressure filtration while washing the distilled water using 3M paper, and the filtered solution was lyophilized.

The lyophilized extract was placed in a 50 ml conical tube, wrapped in aluminum foil, and stored in a -20 ° C freezer.

[Example 5] Preparation of sea cucumber extract using protamex

The extract was prepared by the same procedure except that 200 mg of Protamex and 100 mg of papain were used as the enzyme in Example 4 and used.

[Example 6] Production of sea cucumber extracts using protamex

The extract was prepared by the same procedure except that 200 mg of Protamex and 100 mg of Protex were used as the enzyme in Example 4 and used.

[Example 7] Preparation of sea cucumber extract using protamex

The extract was prepared by the same procedure except that 200 mg of Protamex, 100 mg of papain and 100 mg of Protex were mixed and used as enzyme in Example 4.

[Example 8] Preparation of sea cucumber extract using protamex

The extract was prepared by the same procedure except that the treatment was conducted for 6 hours at a low frequency of 10 kHz in the process of reacting protamex in Example 4.

sample Enzyme decomposition + freeze-dried sample weight yield Example 4 17.9 g 59.7% Example 5 18.1 60.3% Example 6 18.2 60.7% Example 7 18.5 61.7% Example 8 19.3 64.3%

The extract of sea cucumber obtained by ethanol extraction method was used as a control. The experimental procedure was as follows. The results are shown in Table 3.

First, 70% ethanol was prepared with 1400 ml ethanol and 600 ml distilled water. 410 g of the obtained sea cucumber powder sample and 2000 ml of 70% ethanol were put into a triangular flask. The inlet was covered with foil, and sonication was performed for 2 hours. Then, the solution was extracted overnight at room temperature and filtered using a 300 mm filter paper (ADVANTEC.LOT NO.70712907). The obtained extract was put into a vacuum concentrator, and then only ethanol was concentrated under reduced pressure. After extracting only water, it was frozen in Deep Freezer and then freeze-dried.

Dry sea cucumber weight Weight after ethanol extraction Weight after freeze drying yield 410g 670.77g (including water) 49.3g 12.024%

As a result of the above experiment, it was confirmed that the yield of the extraction method using the protease enzyme was much better than the extraction method using ethanol.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims. It can be understood that

Claims (7)

(1) adding protease to the sea cucumber powder to perform enzymatic degradation;
(2) filtering the enzyme hydrolyzate obtained in the step (1); And
(3) lyophilizing the filtrate obtained in the step (2);
≪ / RTI >
The method according to claim 1,
The sea cucumber powder is prepared by first removing raw sea cucumbers and then matured.
Cryopreservation of the first matured sea cucumber;
Secondly dipping the cryo-cryopreserved frozen; And
Drying and crushing the second matured sea cucumber;
The method of claim 1,
3. The method according to claim 2, wherein drying and pulverizing the second-
Dried sea cucumber is dried in a dry oven, and then the expanded sea cucumber is expanded at 10 to 13 kg / cm 2 and at 110 to 130 ° C for 2 to 10 minutes using a puffing device, and then pulverized using a blender ≪ / RTI >
The method according to claim 1,
Wherein the enzyme decomposition of step (1) is carried out at 35 to 45 ° C for 5 to 10 hours.
3. The method of claim 2,
Wherein the sea cucumber powder is passed through a 2 to 3 mm mesh.
The method according to claim 1,
The enzyme is mixed and compounded at a ratio of 1: 0.5: 1 to 1: 1: 1: 0.5: 1 to 1: 1, or ProTamex: Papain (or ProTex) Wherein the method comprises the steps of:
The method according to claim 1,
Wherein the enzyme is treated with a low frequency of 1 to 10 kHz for 6 to 7 hours.

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