KR20160001131A - COSMETIC COMPOSITION COMPRISING Aconogonum polymorphum EXTRACT FOR SKIN WHITENING - Google Patents

Abstract

The present invention relates to a skin whitening cosmetic composition comprising a skin whitening component derived from a Morus alba for. pendula extract or an Aconogonum polymorphum extract as an active component. The skin whitening cosmetic composition of the present invention comprises the skin whitening component derived from a Morus alba for. pendula extract or an Aconogonum polymorphum extract as an active component to have an skin whitening effect by suppressing an activity of tyrosinase which is an enzyme important to a melanin production mechanism, thereby being used in skin whitening cosmetics.

Description

COSMETIC COMPOSITION COMPRISING ACONOGONUM POLYMORUM EXTRACT FOR SKIN WHITENING [0002]

The present invention is based on the finding that the morula alba for . pendula extract or Acinogonum The present invention relates to a whitening cosmetic composition comprising a mulberry extract and a polymorphum extract. More particularly, the present invention relates to a whitening cosmetic composition comprising a whitening active ingredient derived from a perennial mulberry extract or a Singai extract and inhibiting the activity of tyrosinase, To a whitening cosmetic composition capable of exhibiting an excellent whitening effect.

Human skin color is largely determined by the amount of melanin, hemoglobin, carotene, etc. Among them, melanin synthesized by melanin-forming cells in the basal cell layer of the epidermis plays the most important role. Melanin can be divided into eumelanin and pheomelanin. Eumelanin is a granular pigment that is darker in color and darker in color, larger in size and more abundant in Asian or African skin. Pheomelanin is a reddish brown (yellow) light color, small in size and abundant in white skin. Melanin is produced by enzymatic and nonenzymatic oxidation of tyrosine in melanocytes in the basal layer of the epidermis. The mechanism of melanogenesis is tyrosine, a tyrosine enzyme, which produces DOPAquinone, which undergoes autoxidation and enzymatic reaction from dopaquinone to produce melanin, a copolymer.

As the causes and mechanisms of melanin production are revealed, it is possible to reduce the production of melanin by mixing substances having the inhibitory effect of tyrosinase, an enzyme involved in melanin production, in cosmetics or inhibiting some reactions during melanin production Methods are commonly used. Representative materials used for this purpose include plant extracts such as ascorbic acid, kojic acid, hydroquinone, and extracts of Cabbage and licorice. However, kojic acid has excellent tyrosinase activity inhibiting action, but has problems in stability such as discoloration and decrease in potency due to aging change when it is added to cosmetics, and skin irritation is high due to its use limit. Ascorbic acid has a low inhibitory effect on tyrosinase activity and is not suitable as an inhibitor of melanogenesis due to its low molecular stability. Hydroquinone has a high stimulation to the skin, which is a safety problem and its usability is limited at present in cosmetic formulations.

However, in the questionnaire survey, it is considered that women's interest in whitening cosmetics is so high that Korean women are ranked first among the skin troubles that they want to solve first, and that skin pigmentation is so high that the whitening effect is excellent, Development of a cosmetic composition is urgent. Accordingly, the inventors of the present invention have studied a plant component containing a whitening active ingredient.

There are about 4,500 kinds of plants in Korea. These plants are crops, and many plants have high development potential and development necessity. Recently, with the development of natural science, interest in these resource plants has increased, Research is actively being carried out. The inventors of the present invention have found that, among the plants native to Korea, Morus alba for . pendula ) and Singh ( Aconogonum polymorphum) And confirming the effect thereof, thereby completing the present invention.

It is an object of the present invention to provide a whitening cosmetic composition having excellent whitening effect using a natural plant extract, and more specifically, to provide a whitening cosmetic composition containing a whitening active ingredient derived from a perennial mulberry extract or Sinai extract as an active ingredient, The present invention provides a whitening cosmetic composition capable of inhibiting tyrosinase enzyme which is an important enzyme in a mechanism and inhibiting melanin production to exhibit a whitening effect.

In order to achieve the above object, the present invention provides a whitening cosmetic composition comprising, as an active ingredient, a whitening active ingredient derived from a perennial mulberry extract or Sinai extract.

Since the whitening cosmetic composition of the present invention contains the whitening active ingredient derived from the dried mulberry or ssingae extract as an active ingredient, it inhibits the activity of tyrosinase, which is an important enzyme in the melanin production mechanism, and inhibits melanin production, thereby achieving a remarkable whitening effect Therefore, it can be usefully used in cosmetics showing a whitening effect.

FIG. 1 is a graph showing the results of inhibition of tyrosinase activity by drooling mulberry extract. FIG.
2 is a graph showing the results of inhibition of tyrosinase activity by the extract of Singh of the present invention
3 is a graph showing the cell survival rate of the perennial mulberry extract of the present invention.
4 is a graph showing the cell survival rate of the extract of Sinai of the present invention.
5 is a graph showing the results of inhibition of melanin activity by the perennial mulberry extract of the present invention.
FIG. 6 is a graph showing the results of inhibition of melanin activity by the extract of Sinai of the present invention.

The whitening cosmetic composition of the present invention can be used as a cosmetic ingredient for the skin of Morus alba for . pendula extract or Acinogonum polymorphum ) extract as an active ingredient.

The whitening cosmetic composition as described above can exhibit a whitening effect by containing a perennial mulberry extract or an extract of Singh as an active ingredient. More specifically, it contains a whitening active ingredient derived from the perennial mulberry extract or Sinai extract as an active ingredient, Thereby exhibiting an inhibitory effect on tyrosinase activity and exhibiting an effect of suppressing melanin biosynthesis.

Morus alba for pendula is ovate or long ovate ovate with 3 ~ 5 branches, dull or peak, deep heart bottom, length 10cm, dull serrate on edge, pointed end, Is rough or smooth and has a hairs on the back vein. The petiole is 2 to 2.5 cm long and has fine hairs. The fruit is liquid (liquid quality) and ripens in black in June. Fruit ear is spherical or elliptical with a length of 1 to 2.5 cm. Flower is bloomed in June as an iris, and the flower of a male flower hangs on a tail horn that hangs down from the leaf of the lower part of a new branch. The female flower has a length of 5 ~ 10 mm, a light oval, 4 flowers, Is short. The nest is hairless. Shippers are shorter than mulberry trees. The bark is gray brown. The base is grayish brown or grayish white and has fine hairs, but gradually disappears and branches are bent downward. Mycosis (root skin) is called mallow white (桑 白皮).

The above-mentioned Aconogonum polymorphum is also referred to as a peony leaf. It grows at the foot of the mountain and is about 1 meter high. The outpost is thick and straight. Branches are split a lot and have no hair. Leaves are slanting and small, 12 ~ 15 cm long. Both ends are narrow and wavy sawtooth on edge. The leaflet-like stipule is membranous (translucent like thin paper), with hair and veins, and soon split. Flowers bloom in June-August, white, with axilla on the upper part and a large conical flower on the end of the branch. Bracts are small and each have two or three flowers and are longer than small flower sacks. The shrub fragments are about 3mm in length. There are 5 calyxes and 8 stamens. The fruit is 3mm long and about 5mm long, about twice the length of the horn, ripened in October. Breeding is done by seeding or giving up. Childhood is sour and eats raw. It is distributed in Korea and China.

The perennial mulberry extract may be at least one selected from the group consisting of plant extracts, leaf extracts, stem extracts, fruit extracts, root extracts, seed extracts and twigs extracts of the perennial mulberry, Or a mixture thereof.

The Sinai extract may be at least one kind selected from the group consisting of a seedling extract, a leaf extract, a stem extract, a fruit extract, a root extract, a seed extract and a twig extract of the sina, ≪ / RTI >

The perennial mulberry extract or Sinai extract may be one selected from the group consisting of water, methanol, ethanol, propanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, chloroform, , Or an extract obtained by extracting with a mixed solvent of two or more kinds. Preferably, the extract may be extracted with one kind of solvent selected from the group consisting of water, methanol, ethanol, hexane, and butylene glycol, And more preferably, an extract obtained by extracting with methanol. Especially, the extract obtained by extracting with methanol can exhibit more excellent tyrosinase activity inhibiting effect.

As a result of measuring the inhibitory effect of tyrosinase activity of the perennial mulberry extract or Sinai extract, it is possible to achieve a high tyrosinase activity inhibitory effect even at a low concentration as compared with arbutin having a known tyrosinase production inhibitory effect.

The above-mentioned perennial mulberry extract or Sinai extract is prepared by heating the above-mentioned solvent at 45 to 120 ° C for 30 minutes to 24 hours with hot water extraction, and dipping at 5 to 40 ° C for 30 minutes to 15 days for shaking , And the use of the above method is preferable because the yield of the extract can be improved.

The amount of the extraction solvent to be used is not particularly limited. However, if the amount of the extraction solvent is too small compared to the above-mentioned perennial mulberry extract or Singhin plant, the extraction can not be sufficiently extracted. If an excessive amount is used, And it becomes costly.

At this time, when hot water extraction is carried out, it is necessary to sufficiently include the suspended mulberry sample and perform extraction in a warmed state in order to increase the extraction efficiency. If the temperature is too low, it is difficult to fully utilize the advantage of warming, and it takes a long time for extraction. If the temperature is too high, decomposition of useful materials such as whitening active material may occur. In case of shaking, it can be extracted at room temperature. The sample in powder form is immersed in a sufficient extraction solvent for a sufficient time, for example, about 3 days, so that the extraction can be sufficiently performed. If the extraction is not sufficient, Or increase the extraction volume by increasing the extraction time.

The perennial mulberry extract or Sinai extract extracted from the above method may be contained in an amount of 0.001 to 30% by weight based on the total weight of the whitening cosmetic composition.

Preferably, the perennial mulberry extract may be contained in an amount of 0.005% by weight or more based on the total weight of the whitening cosmetic composition, and the extract of Singh can be contained in an amount of 0.01% by weight or less based on the total weight of the whitening cosmetic composition, The perennial extract of Mulberry and the extract of Sina may be contained in an amount of 0.005 to 0.01% by weight based on the total weight of the whitening cosmetic composition.

If the perennial mulberry extract or the extract of Sinai is less than 0.005% by weight based on the total weight of the whitening cosmetic composition, the whitening activity effect can not be sufficiently obtained and the function as a skin whitening cosmetic is deteriorated. If it exceeds 0.01% by weight, There is a problem of safety due to the above.

Since the perennial mulberry extract or Sinai extract prepared from the above method contains a whitening active ingredient, it can exhibit a remarkable effect of inhibiting tyrosinase activity and inhibiting melanin biosynthesis.

The preferred formulation of the whitening cosmetic composition of the present invention is not particularly limited, but may be any one selected from the group consisting of softening agent, nutritional lotion, nutritional essence, nutritional cream, body cream, and pack.

Hereinafter, the present invention will be described in more detail with reference to Examples. The present invention is intended to more specifically illustrate the present invention, and the scope of the present invention is not limited to these embodiments.

Example

Throughout this specification, "%" used to denote the concentration of a particular substance is intended to include solids / solids (wt / wt), solid / liquid (wt / The liquid / liquid is (vol / vol)%.

end. From the drooping mulberry tree Derived  Extract preparation

< Example  1> Heat number  extract

The stalks of drooping mulberry were placed in purified water of 5 times the total weight and subjected to hot water extraction at 105 ° C for 5 hours. When the extraction process is finished, it is filtered with Whatman paper No. 1, re-filtered through 0.45 ㎛ membrane filter (TF-450, 60173, PALL Life Sciences) rotary vacuum evaporator), followed by lyophilization. Thus, 15.3 g of dried mulberry hydrothermal extract based on dry weight was obtained.

< Example  2> Methanol extract

The extracts were filtered with a filter paper and filtered through a 0.45 ㎛ membrane filter (Supor®). The filtrate was filtered through a filter paper, -450, 60173, PALL Life Sciences), concentrated using a rotary vacuum evaporator, and lyophilized to obtain 20.04 g of a methanol extract from the dried mugwort based on the dry weight.

< Example  3> Ethanol extract

The extracts were filtered with filter paper and filtered through a 0.45 ㎛ membrane filter (Supor®). The extracts were washed three times with distilled water of distilled mulberry stem and extracted three times with 70% ethanol at 25 ° C for 72 hours. -450, 60173, PALL Life Sciences), concentrated using a rotary vacuum evaporator, and lyophilized to obtain 6.78 g of dried mulberry ethanol extract based on dry weight.

< Example  4> Butylene glycol  extract

The extracts were filtered through filter paper and filtered through a membrane filter (0.45 ㎛). The filtrate was filtered through a filter paper Supor®-450, 60173, PALL Life Sciences).

< Example  5> Propylene glycol  extract

(50% propylene glycol) was added to the stem of the perennial mulberry tree, and the mixture was shaken at 25 ° C for 72 hours. The extract was filtered through filter paper and filtered through a 0.45 ㎛ membrane filter Supor®-450, 60173, PALL Life Sciences).

I. From Singh Derived  Extract preparation

< Example  6> Heat number  extract

Sinai outpost was placed in purified water of 5 times the total weight and subjected to hot water extraction at 105 ° C for 5 hours. When the extraction process is completed, it is filtered with Whatman paper No. 1, re-filtered with 0.45 μm membrane filter (TF-450, 60173, PALL Life Sciences) and then firstly washed with a rotary vacuum evaporator Concentrated and then lyophilized. Thereby, 12.1 g of dry hot water extract was obtained.

< Example  7> methanol extract

The extracts were filtered with filter paper and filtered through a 0.45 μm membrane filter (Supor®- 450, 60173, PALL Life Sciences), concentrated using a rotary vacuum evaporator, and lyophilized to obtain 17.2 g of a methanol extract of Shinma based on the dry weight.

< Example  8> Ethanol extract

The extract was filtered through filter paper and filtered through a 0.45 ㎛ membrane filter (Supor-R). The filtrate was filtered through a filter paper, 450, 60173, PALL Life Sciences), concentrated using a rotary vacuum evaporator, and lyophilized to obtain 4.9 g of a pure ethanol extract on a dry weight basis.

< Example  9> Butylene glycol  extract

The extract was filtered through filter paper and filtered through a 0.45 ㎛ membrane filter (Supor). The filtrate was filtered through a filter paper, &Lt; / RTI &gt; ®-450, 60173, PALL Life Sciences).

< Example  10> Propylene glycol  extract

The extract was filtered through a filter paper and filtered through a 0.45 ㎛ membrane filter (Supor). The filtrate was filtered through a filter paper, &Lt; - &gt; 450, 60173, PALL Life Sciences).

< Experimental Example  1> Tyrosinase  Evaluation of efficacy of extracts according to active inhibition effect

1. Evaluation by site

The inhibitory effect of tyrosinase activity on the methanol extracts of the respective parts of the perennials and the birds produced in Example 2 and Example 7 was measured. At this time, tyrosinase (T3824-50KU) was isolated and purified from mushroom and purchased from Sigma.

The methanol extract of each part of the perennial mulberry and syringe prepared in Example 1 and Example 6 was dissolved in DMSO (dimethyl sulfoxide, Wako), further diluted in a buffer solution (0.1 M Phosphate buffer pH 6.5) Were prepared and used. At this time, arbutin was used as a control. A buffer solution was added to a 96-well plate, and a substrate solution (1.5 mM tyrosine solution) was added. An extract sample solution was added thereto, 2000 U / ml tyrosinase was added, and the mixture was reacted at 37 ° C for 1 hour. At this time, the control group was prepared by adding buffer solution instead of each extract.

After completion of the reaction, the absorbance at a wavelength of 490 nm was measured using a microplate recorder. The tyrosinase inhibitory effect of the extract by concentration was calculated by the following equation (1).

[Equation 1]

(%) = {1- (b-b ') / (a-a')} 100

(A: absorbance after the reaction of the blank solution, b: absorbance after reaction of the sample solution and a ', b': absorbance measured by replacing with buffer solution instead of tyrosinase)

Table 1 below shows the inhibition rate of tyrosinase activity of the methanol extract of the perennial mulberry produced in Example 2 above.

Evaluation of inhibitory activity of tyrosinase activity of methanol extracts of perennial mulberry Example 2 sample Arbutin leaf stem Fruit Root strain Concentration (/ / ml) 500 100 100 100 100 100 Inhibition rate of tyrosinase activity (%) 45 50 89 20 25 44

From the results shown in the above Table 1, the methanol extract of the perennial mulberry produced in Example 2 showed the highest inhibition rate of tyrosinase activity in the extract of the stem of the mulberry stem. The above results showed that the tyrosinase activity was inhibited at a significantly lower concentration than that of the control arbutin.

Table 2 shows the inhibition rate of tyrosinase activity of the methanol extract of each of the strains prepared in Example 7.

Example 7 sample Arbutin leaf stem Fruit Root outpost Concentration (/ / ml) 500 100 100 100 100 100 Inhibition rate of tyrosinase activity (%) 46 35 42 12 19 60

From the results shown in the above Table 2, the extruded extracts of Sinai showed the highest inhibition rate of tyrosinase activity in the methanol extracts of the Sinai region prepared in Example 7. The above results showed that the tyrosinase activity was inhibited at a significantly lower concentration than that of the control arbutin.

2. Extraction solvent evaluation

As shown in the above Table 1, the inhibitory effect of tyrosinase activity on the stem portion having the highest inhibition rate of tyrosinase activity was determined by the extraction solvent.

Table 3 shows the inhibition rate of tyrosinase activity according to the extraction solvent of the stem of the mulberry stem.

division Concentration (/ / ml) Inhibition rate of tyrosinase activity (%) Comparative Example 1 (Arbutin) 500 45 Example 1 100 60 Example 2 100 89 Example 3 100 34 Example 4 100 22 Example 5 100 24

From the above results, when the methanol extract was used as the extraction solvent, the highest inhibitory activity of tyrosinase activity was observed at the stigmata of the succulent mulberry stem with high inhibition rate of tyrosinase activity.

As shown in the above Table 2, inhibition activity of tyrosinase activity was measured for each of the extractants for the outbreaks of tyrosinase activity inhibition rate in the extracts of Singh.

Table 4 below shows the results of evaluation of the inhibition rate of tyrosinase activity according to the extraction solvent of the Sinai outpost.

division Concentration (/ / ml) Inhibition rate of tyrosinase activity (%) Comparative Example 1 (Arbutin) 500 46 Example 1 100 31 Example 2 100 60 Example 3 100 44 Example 4 100 21 Example 5 100 12

From the above results, the syringe shoots having a high inhibition rate of tyrosinase activity showed the highest inhibition rate of tyrosinase activity when methanol was used as an extraction solvent.

3. Evaluation by concentration

In Experiment 1, the inhibitory effect of tyrosinase activity on the methanol extract of the stem of the perennial mulberry stem, which has the highest inhibitory activity against tyrosinase activity, was measured.

Table 5 and FIG. 1 show inhibition rates of tyrosinase activity by concentration of methanol extracts at the stalks of the perennial mulberry stem of Example 2. At this time, in the case of arbutin of Comparative Example 1 as a control group, inhibition rate of tyrosinase activity was observed at 49% at a concentration of 500 占 퐂 / ml.

Concentration (/ / ml) Inhibition rate of tyrosinase activity (%) 100 89 75 85 50 76 25 49 10 -3 5 -6 One -18

From the results shown in Table 5, it was confirmed that the tyrosinase inhibitory activity was excellent even at a significantly lower concentration than that of the conventional tyrosinase inhibitory substance arbutin of Comparative Example 1.

In addition, the inhibitory activity of tyrosinase activity was shown in Table 6 and FIG. 2 according to the concentration of the extract of Sinai outpost of Example 7. At this time, in the case of arbutin of Comparative Example 1 as a control group, a tyrosinase inhibiting rate of 45% was observed at a concentration of 500 占 퐂 / ml.

Concentration (/ / ml) Inhibition rate of tyrosinase activity (%) 100 60 75 29 50 4.7 25 -6 10 -11 5 -14 One -21

From the results shown in Table 6, it was confirmed that the tyrosinase inhibitory activity was excellent even at a significantly lower concentration than that of the conventional tyrosinase inhibitory substance arbutin of Comparative Example 1.

< Experimental Example  2> Evaluation of efficacy of extracts according to the inhibitory effect on melanin formation

1. Cytotoxicity test

The cytotoxicity of the perennial mulberry stem and the pupae obtained from the method of Example 2, which is considered to be the most effective in the extraction process and efficiency of the above examples, were measured.

In order to examine the cytotoxicity of the extract according to the present invention, B16F1 melanoma cells were dispensed in 96-well plates at an appropriate concentration, cultured in a CO ₂ incubator at 37 ° C for 24 hours Respectively. At this time, DMEM / high glucose (dulbeco's modified eagle medium, Hyclone) containing 10% bovine serum and 1% antibiotics was used. After culturing, the extract was treated at different concentrations and cultured for 72 hours under the same culture conditions. After the culture was completed, the culture solution was removed and the culture solution containing 5 ㎎ / ㎖ of Neutral red reagent was added and cultured for 2 hours. The culture was then removed, treated with 1% CaCl ₂ and formaldehyde, shaken at 100-1000 rpm for several minutes, and then discarded. Then, 1% acetic acid and 50% ethanol were added to each well, shaken well for 5 to 20 minutes, and then the absorbance at 570 nm / 655 nm was measured using a microplate reader. Are shown in Table 7 and Fig.

Concentration (/ / ml) Cell survival rate (%) 100 93 75 112 50 114 25 106 10 101 5 95 One 95 0.1 98 0.01 105

As a result of the cytotoxicity test of Table 7, the methanol extract of the stigmatized mulberry stem showed no cytotoxicity at a concentration of 100 μg / ml or less.

In the same experimental procedure as described above, the methanol extract of the Sinai outpost of Example 7 was dissolved in DMSO, and the cytotoxicity of the extract obtained by diluting the extractant according to each experimental method was shown in Table 8 and FIG.

Concentration (/ / ml) Cell survival rate (%) 100 92 75 107 50 105 25 102 10 96 5 97 One 96 0.1 103 0.01 104

As a result of the cytotoxicity test of the above Table 8, the methanol extract of the Sinai outpost also showed no cytotoxicity at a concentration of 100 μg / ml or less.

2. Measurement of inhibition of melanin formation

B16F1 melanoma cells were cultured in a 6-well plate at a correct concentration for 24 hours, and the extract and arbutin were added to the culture medium of B16F1 melanoma cells as a test substance at the indicated concentrations, and cultured for 3 days. At this time, melanocyte-stimulating hormone (a-MSH) was treated at an appropriate concentration to induce melanin synthesis, and arbutin-treated group, which is known to exhibit melanin synthesis inhibiting effect, was used as a control group .

After culturing for 48 hours, the culture medium was removed, washed with PBS, treated with trypsin, transferred to a tube (ep-tube) to collect all cells, washed with PBS, dried and dissolved in 1N NaOH and 10% DMSO solution, After transferring to a plate, the absorbance at 490 nm was measured using a microplate recorder.

The results are shown in Table 9 and FIG.

sample α-MSH (-) α-MSH (+) Arbutin Example 2 Concentration (/ / ml) 500 100 50 25 Melanin synthesis rate One 1.8 1.06 0.31 1.03 2.25

As a result, it can be seen that the extract of the mulberry stem which is entrapped through Table 9 and FIG. 5 shows a whitening effect by confirming that the melanin synthesis is inhibited in a concentration-dependent manner.

Table 10 and FIG. 6 show the results of inhibiting the melanin formation by dissolving the methanol extract of the Sinai herbaceous plant of Example 7 in DMSO and diluting it with the concentration of the extraction solvent according to each experimental method.

sample α-MSH (-) α-MSH (+) Arbutin Example 7 Concentration (/ / ml) 500 100 50 25 Melanin synthesis rate One 1.8 1.1 0.55 1.75 2.18

As a result, as shown in Table 10 and FIG. 6, it was confirmed that melanin synthesis inhibition was inhibited in a concentration-dependent manner as a result of showing the results of inhibition of melanin formation by the Singa outpost extract of Example 7 of the present invention, Respectively.

Formulation Example

The following is an example of the composition of a cosmetic composition as a formulation having a whitening effect by containing sallow mulberry or sine extract in each cosmetic formulation. The formulation examples are as follows. Here, the perennial mulberry stem extract of Example 2 or the sine outpost extract of Example 7 was used.

Formulation Example  1: Manufacture of softening longevity

The composition of the formulation example of the softening longevity in the cosmetic containing the above extract is shown in Table 11 below.

ingredient Content (% by weight) Example 2 or Example 7 0.01 Polyoxyethylene hardened castor oil 0.5 Glycine 3.0 Dipotassium glycyrrhizate 0.1 1,3-butylene glycol 3.0 Sodium hyaluronate 1.0 ethanol 5.0 Antioxidant 0.1 Triethanolamine 0.1 EDTA 0.1 Purified water Remaining amount (to 100)

Formulation Example  2: Manufacture of nutrition lotion

The composition of the formulation of the nutritional lotion among the cosmetic compositions containing the extract is shown in Table 12 below.

ingredient Content (% by weight) Example 2 or Example 7 0.01 glycerin 7.0 Sikhitane Stearate Sucrose Oz cocoate 2.0 Mineral oil 4.0 Trioctanoin 1.0 Stearic Acid 1.0 Glyceryl stearate 0.5 Sorbitan monostearate 1.0 Dimethicone 0.5 Antioxidant 0.3 Triethanolamine 0.1 Carbomer 2.0 EDTA 0.1 Purified water Remaining amount (to 100)

Formulation Example  3: Manufacture of nutritional essence

Formulation examples of nutritional essence among cosmetics containing the above extract are shown in Table 13 below.

ingredient Content (% by weight) Example 2 or Example 7 0.01 glycerin 5.0 1,3-butylene glycol 2.0 Polyethylene glycol 2.0 Carbomer 1.0 Sodium hyaluronate 0.1 Glycine 3.0 Polyacrylamide 2.0 Hydroxyethylcellulose 0.2 ethanol 3.0 Antioxidant 0.3 Triethanolamine 0.1 Polyoxyethylene hardened castor oil 1.0 EDTA 0.1 Purified water Remaining amount (to 100)

Formulation Example  4: Manufacture of nutritional cream

Formulation examples of the nutritional cream among the cosmetics containing the above extract are shown in Table 14 below.

ingredient Content (% by weight) Example 2 or Example 7 0.01 1,3-butylene glycol 3.0 glycerin 3.0 Hydrogenated lecithin 1.0 Octyldodecanol 3.0 Trioctanoin 2.0 Stearic Acid 1.5 Cetostearyl alcohol 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 2.0 Dimethicone 3.0 Antioxidant 0.3 Xanthan gum 0.2 Triethanolamine 0.1 EDTA 0.1 Purified water Remaining amount (to 100)

Formulation Example  5: Body cream  Produce

Formulation examples of the body cream in cosmetics containing the above extract are shown in Table 15 below.

ingredient Content (% by weight) Example 2 or Example 7 0.01 1,3-butylene glycol 6.0 glycerin 4.0 Sodium cetyl sulfate 0.1 Panthenol 1.0 Xanthan gum 1.0 Carbomer 0.1 Bis wax 0.3 Cetanol 1.5 Glyceryl stearate 2.0 Sorbitan stearate 1.5 Mineral oil 5.0 Squalene 2.0 Antioxidant 0.3 Dimethicone 1.0 Triethanolamine 0.1 EDTA 0.1 Purified water Remaining amount (to 100)

Formulation Example  6: Manufacture of pack

Formulation examples of the cosmetic pack containing the above extract are shown in Table 16 below.

ingredient Content (% by weight) Example 2 or Example 7 0.01 glycerin 6.0 1,3-butylene glycol 4.0 Squalene 0.1 Dimethicone 1.0 Sodium hyaruronate 1.0 Glycine 0.1 Polyacrylamide 0.3 Antioxidant 1.5 Triethanolamine 2.0 EDTA 1.5 Purified water Remaining amount (to 100)

In order to confirm whether the whitening effect shown in the above-mentioned in vitro test actually contributes to skin whitening of human beings, a human body application test was performed on the nutritional cream of Prescription Example 4.

< Experimental Example  3> Skin whitening effect test

1. Measurement of Melanin Index

The melanin index was measured using Mexameter MX18 before use, 1 week after use, 2 weeks after use, and 4 weeks after use. The lower the melanin index, the better the melanin. In this study, repeated measurements of the skin surface at the same site three times in each measurement were used as average values. The results are shown in Table 17 below.

Melanin index 0W 2W 4W 206.00 198.67 190.33

As a result of measuring the melanin index of the nutritional cream containing the extract, the melanin index tended to decrease from the 2nd week of administration and it was confirmed that the melanin was improved.

2. Measure skin brightness

Skin brightness was measured before, after 1 week, 2 weeks and 4 weeks after using Spectrophotometer CM600d. The skin brightness is evaluated as the L value, and the higher the L value, the more the skin is brightened. In this study, repeated measurements of the skin surface of the same area three times at each measurement were used as average values. The results are shown in Table 18 below.

L * 0W 2W 4W 60.76 61.17 61.58

As a result of measuring the brightness of the skin against the nutritional cream containing the extract, the L * value tended to increase from the 2nd week onwards, and the skin brightness was improved.

Therefore, it was confirmed that the nutritional cream containing the extract reduced the melanin index and increased skin brightness to help skin whitening.

Claims (6)

Drifting Mulberry ( Morus alba for . pendula extract or Aconogonum polymorphum extract as an active ingredient.
The method according to claim 1,
The perennial mulberry extract is a stem extract, a twig extract, or a mixture thereof of the perennial mulberry,
Wherein the Sinai extract is an outpost extract, a twig extract, or a mixture thereof of the Sina.
The method according to claim 1,
Wherein the perennial mulberry extract or sine extract is obtained by extracting with water or a solvent selected from the group consisting of alcohol, methanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, chloroform, glycerin, Wherein the cosmetic composition is an extract.
The method according to claim 1,
A method of extracting the perennial mulberry extract or Sinai extract by heating at 45 to 120 ° C for 30 minutes to 24 hours and a method of extracting by shaking at 5 to 40 ° C for 30 minutes to 15 days is used &Lt; / RTI &gt;
The method according to claim 1,
Wherein the whitening cosmetic composition comprises 0.001 to 30% by weight based on the total weight of the whitening cosmetic composition.
The method according to claim 1,
Wherein the whitening active ingredient has a tyrosinase activity inhibiting effect and a melanin biosynthesis inhibiting effect.

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