KR20150146157A - Animal planktons culturing apparatus - Google Patents

Abstract

The present invention relates to an animal plankton culturing apparatus comprising a culturing body where animal plankton and a culturing solution are stored; a feed supply part formed on the upper part of the culturing body, and supplying feed to the culturing body; an air blow part supplying oxygen inside the culturing body; and an underwater heater arranged inside the culturing solution of the culturing body, and controlling temperature of the culturing solution, wherein the culturing body includes an animal plankton outlet arranged on the middle area of total height, and discharging the cultured animal plankton to the outside; and a culturing solution inlet arranged on the upper area, and supplying the culturing solution to the inside, and the air blowing part is arranged higher than an animal plankton discharging pipe.

Description

{ANIMAL PLANKTONS CULTURING APPARATUS}

The present invention relates to a zooplankton culture apparatus, and more particularly, to a zooplankton culture apparatus capable of stably culturing zooplankton at a high concentration.

In the reservoir and freshwater lake, which are formed according to the purpose of using water resources such as domestic water, industrial water, agricultural water and so on, there is frequent occurrence of green tide due to the influx and accumulation of nutrients such as nitrogen and phosphorus, increase of water temperature and increase of daylight. In order to reduce the number of phytoplankton species that cause green algae as a phytoplankton such as cyanobacteria, algae, brown algae, and monocotyledonous birds which cause such algae phenomenon, various techniques using animal plankton have been studied. The zooplankton is a higher level organism than the phytoplankton in the aquatic food net structure. It is able to control the algae phenomenon by mass-proliferating it in the water system under a certain condition. In order to apply this technology to the field and use it, artificial plankton . ≪ / RTI >

The plankton culture apparatus is used for culturing and propagating zooplankton. Patent No. 10-0903691 discloses a device for culturing zooplankton. The disclosed zooplankton culture apparatus is somewhat complicated in structure and requires a large site for facility installation.

An object of the present invention is to provide a zooplankton culture apparatus capable of safely mass-culturing zooplankton without impacting zooplankton while physically supplying oxygen.

Another object of the present invention is to provide a zooplankton culture apparatus capable of mass production of zooplankton at low production costs without loss or corruption of food for propagation.

It is another object of the present invention to provide a zooplankton culture apparatus capable of producing zooplankton at high density by setting environmental conditions at suitable water temperature, suitable oxygen supply amount, and food supply amount capable of mass production of zooplankton.

The above objects and various advantages of the present invention will become more apparent from the preferred embodiments of the present invention by those skilled in the art.

The object of the present invention can be achieved by a zooplankton culture apparatus. A zooplankton culture apparatus of the present invention comprises: a culture main body containing zooplankton and a culture solution; A food supply unit provided at an upper portion of the culture body to supply food to the culture body;

An acid base for supplying oxygen into the culture body; And an underwater heater disposed inside the culture medium of the culture medium to control the temperature of the culture medium. The culture medium includes an animal plankton outlet for discharging the cultured animal plankton to the outside in the middle of the entire height; And a culture liquid inlet which is disposed in an upper area and supplies a culture liquid to the inside, wherein the acid generator is disposed at a higher position than the zooplankton discharge tube.

According to one embodiment, the underwater heater maintains the culture at 24 ° C to 26 ° C.

According to one embodiment, the food supply unit feeds the food at a daily dose of 0.84 ml / Lday to 0.92 ml / Lday.

According to one embodiment, the acid part supplies oxygen at 4cc / Lday to 5.6cc / Lday.

According to one embodiment, the lower part of the culture body gradually becomes smaller in area, and a sediment discharge pipe for discharging the sediment contained in the culture liquid to the outside is formed.

The zooplankton culture apparatus according to the present invention can cultivate zooplankton at a high concentration by feeding food and oxygen and adjusting the temperature of the culture liquid to a value suitable for culturing zooplankton.

In particular, it is possible to maximize the cultivation of zooplankton by supplying oxygen in a maximum amount that does not affect the activity of zooplankton when supplied.

In addition, it is possible to maintain the environment suitable for the culture of zooplankton by reducing the concentration of ammonia and the pH by periodically discharging the precipitates generated in the culture of zooplankton to the outside.

1 is a perspective view showing a configuration of a zooplankton culture apparatus according to the present invention,
FIG. 2 is a schematic view showing a culturing process of the zooplankton culture apparatus according to the present invention,
FIGS. 3A to 4B are graphs showing experimental results of the culture conditions of the zooplankton culture apparatus according to the present invention. FIG.

For a better understanding of the present invention, a preferred embodiment of the present invention will be described with reference to the accompanying drawings. The embodiments of the present invention may be modified into various forms, and the scope of the present invention should not be construed as being limited to the embodiments described in detail below. The present embodiments are provided to enable those skilled in the art to more fully understand the present invention. Therefore, the shapes and the like of the elements in the drawings can be exaggeratedly expressed to emphasize a clearer description. It should be noted that in the drawings, the same members are denoted by the same reference numerals. Detailed descriptions of well-known functions and constructions which may be unnecessarily obscured by the gist of the present invention are omitted.

FIG. 1 is a perspective view showing the construction of a zooplankton culture apparatus 100 according to the present invention, and FIG. 2 is a schematic view schematically showing an operation process of a zooplankton culture apparatus 100.

As shown, the zooplankton culture apparatus 100 includes a culture body 110 in which the zooplankton M and the culture liquid are received and the zooplankton M is cultured, a culture medium 110 for supplying the culture medium to the culture body 110 A feeding part 130 for feeding food to the culture body 110; an underwater heater 140 for adjusting the temperature of the culture liquid; a acid part (for example, 150).

The culture body 110 is provided in the form of a circular cylinder having a receiving space. The culture body 110 is supported so as to stand on the ground by the support frame 112. A culture medium inlet pipe 111 for feeding the culture medium into the culture main body 110 is formed at one side of the upper side of the culture main body 110. The culture medium inflow pipe 111 is connected to the culture medium supply unit 120 to periodically supply the culture medium. The culture medium inlet pipe 111 is provided with a culture medium inlet valve 111a to open or close the culture medium inlet pipe 111 automatically or manually.

An overflow pipe 115 is provided in the direction opposite to the culture body 110 at the same height as the culture medium inflow pipe 111. The overflow pipe 115 allows the height of the culture liquid inside the culture body 110 to be constant.

On the other hand, a zooplankton outlet 113 is formed in the center of the entire height of the culture body 110. The zooplankton cultivation apparatus 100 according to the present invention activates the zooplankton M in the middle and upper areas of the culture main body 110 so that the zooplankton discharge port 113 is formed to facilitate capturing of the zooplankton M, Of the total height of the cultivation main body 110 in the range of 40% to 60%.

The culture solution and the zooplankton M are discharged together through the zooplankton outlet 113 together. At this time, the number of zooplankton to be captured at one time is about 15,000 to 20,000, and only about 10L of the culture liquid is spilled out. At this time, the maximum number of catches per week is limited.

The lower portion of the culture body 110 is formed in a semicircular or inverted triangular shape in cross section so that its area gradually becomes narrower. By this shape, sediments suspended in the culture liquid are precipitated in the lower part of the culture body 110. A sediment discharge pipe 117 is formed in the lower part of the cultivation body 110.

Chlorella, which is injected into the body and the food that is produced by culturing zooplankton, precipitates and sinks over time. When the sediment is accumulated over a predetermined height, the sediment discharge pipe (117) is opened to discharge the sediment to the outside. The precipitate can be periodically removed through the sediment discharge pipe 117 to prevent an increase in the concentration of ammonia generated in the sediment to prevent the pH change.

Although not shown in the drawing, the culture body 110 is provided with a temperature sensor, a sensing sensor, and the like for sensing the temperature of the culture liquid and the concentration of ammonia.

The feeding unit 130 feeds the feed of the zooplankton M by a certain amount to the culture body 110. The amount of food supplied by the food supply unit 130 is controlled according to the control of the control unit 160. Feeding phytoplankton supplied through the food supply unit 130 may be chlorella.

The food supply unit 130 includes a food storage box 131, an air pump 133 for supplying air to the food storage box 131, and a food stirrer 135 for stirring the food. The food supply unit 130 stirs the food by the food stirrer 135 and supplies a predetermined amount set by the controller 160 to the culture main body 110. The feed inlet valve 137a coupled to the feed inlet pipe 137 is opened and closed under the control of the controller 160 to regulate the feeding amount.

The underwater heater 140 heats the culture liquid to maintain the temperature of the culture liquid constant. The underwater heater 140 is disposed in the inside of the culture liquid so that heat can be evenly distributed over the entire region of the culture body 110. It is preferable that the underwater heater 140 is arranged at a predetermined length in the vertical direction in the center region of the culture body 110 because the zooplankton M is located in the middle portion of the culture body 110.

The underwater heater 140 is controlled on / off by the control of the controller 160. The underwater heater 140 is extended from the power supply line 141 to receive power.

The acid base 150 supplies oxygen to the culture medium so that the zooplankton can grow in the culture medium without moving to the water surface. The oxygen base 150 extends into the culture medium to supply oxygen. At this time, the lower end position of the oxygen supply pipe 151 is arranged higher than the animal plankton outlet 113.

That is, as shown in FIG. 2, the height h2 of the lower end of the oxygen supply pipe 151 is arranged to be higher than the height h1 of the animal plankton outlet 113 (h1 <h2). This is to prevent the flow of the zooplankton discharged to the zooplankton outlet 113 by the supply pressure of the oxygen supplied by the oxygen supply pipe 151.

The oxygen supply pipe 151 is located at the top of the zooplankton discharge port 113 to prevent the zooplankton M from being swept away to the supply pressure of oxygen and smoothly moving to the zooplankton discharge port 113.

The amount of oxygen supplied through the acid base 150 is controlled by the control of the controller 160 to the extent that it does not affect the activity of the zooplankton.

The control unit 160 controls the amount of the culture solution supplied from the culture solution supply unit 120, the amount of food supplied to the food supply unit 130, the ON / OFF state of the water heater 140, and the oxygen supply amount of the acid unit 150. The control unit 160 according to the present invention controls the respective components under optimized conditions based on experimental results obtained under various conditions.

FIG. 3A shows experimental results comparing the numbers of zooplankton before and after experimentation when the feeding amounts to different experimental groups were different from each other.

When the temperature of the culture medium was maintained at 22 ° C and the supply rate of oxygen was kept at 100 cc / min, and the feeding amounts were changed to 15 ml, 18 ml, 22 l, 25 ml, After 9 weeks of incubation, the number of zooplankton was increased as the amount of feed was increased. Especially, when the feeding amount of food is 21ml ~ 23ml (0.84ml / L · day ~ 0.92ml / L · day when converted to a day), it can be seen that the increase in population is largest at 1960inds / L.

FIG. 3B is an experimental result comparing the numbers of zooplankton before and after the experiment when the feeding amount of the food and the temperature of the culture liquid were kept constant and the oxygen supply amount was varied in different experimental groups.

The number of animals after the experiment was compared when the feeding amount of the food was kept at 10 ml and the temperature of the culture liquid was kept at 22 ° C. and the supply amounts of oxygen were changed to 0 cc / min, 30 cc / min, 120 cc / min and 150 cc / min. After 11 weeks of incubation, experimental group 1 without aeration showed the lowest DO concentration to 1.8 mg / L, the lowest number of individuals, and the activity of zooplankton was not active.

In the experimental group 2, the number of individuals was not much different from the experimental group 1, but the activity was more active. The final DO concentration was 3.7 mg / L, which was the maximum aeration that did not affect the agitation of zooplankton.

 Experimental group 3 was able to stir, but did not affect the activity of zooplankton. The activity of zooplankton in experiment group 3 was the most active, and the final DO concentration was 5.2 mg / L.

 Experimental group 4 showed an aeration effect (upper layer 1/7 ~ 8) affecting zooplankton in some areas. The zooplankton was not active in the upper part because it affects the activity of zooplankton, and it was observed to be actively active mainly in the upper and middle layers. The final DO concentration was the highest at 5.3. The number of individuals is smaller than that of experimental group 3, which is considered to be due to the decrease in activity area.

Based on these experimental results, it is considered that the supply of oxygen supplied from the acid basin is best supplied from 4cc / L · day to 5.6cc / L · day per day.

FIG. 4A shows experimental results in which the feed amount and the oxygen supply amount are kept constant and the temperature of the culture liquid is made different from each other in different experimental groups.

The feed amount of the feed was kept at 5 ml and the supply amount of oxygen was kept at 60 cc / min. In the experimental group 1, the temperature of the culture liquid was maintained at 20 ° C, the experimental group 2 at 22 ° C, the experimental group 3 at 24 ° C and the experimental group 4 at 26 ° C. As a result of the experiment with the temperature difference of 2 ℃, the change of the population increase during the first two weeks was small.

There was no significant difference in the numbers of the zooplankton when the number of the 3 parking lots was determined, but zooplankton was extinct in the experimental group 3 and the experimental group 4 when the number of the 4 parking lots were grasped. The activity of zooplankton is active when the temperature of the culture is increased, but it is presumed that it does not scatter. In case of spawning, the water heater 140 was turned off and the temperature was lowered after the extinction of zooplankton, but there was no hatching zooplankton.

FIG. 4B is for testing the temperature condition in the same manner as FIG. 4A. In the experiment of FIG. 4A, it is assumed that the zooplankton is annihilated by increasing the temperature difference to 4 ° C. from the third week, This is the result of re-testing population changes for 5 weeks.

There was no significant change in the population during the first two weeks of the retest, but it was confirmed that the number of individuals in the experimental groups 3 and 4 was larger than that in the experimental groups 1 and 2. The temperature experiments were carried out in the total population of the zooplankton population (unlike the aeration and feeding experiments).

Experimental results indicate that maintaining the temperature of the culture medium in the range of 24 ~ 26 ℃ can cultivate high concentrations of zooplankton in consideration of the activity and scattering of zooplankton.

Based on the experimental results, the control unit 160 controls the respective components such that the feed rate is 21 to 23 ml, the oxygen aeration rate is 100 to 140 cc / min, and the culture liquid temperature is 24 to 26 ° C.

A culturing process of the zooplankton culture apparatus 100 according to the present invention having such a configuration will be described with reference to Figs. 1 and 2. Fig.

The culture medium is supplied to the culture body 110 for culturing, and the zooplankton (M) is supplied. The control unit 160 controls the food supply unit 130 to supply a predetermined amount of food. Further, the underwater heater 140 also controls the power supply so that the temperature of the culture liquid is maintained at 24 to 26 占 폚.

The amount of oxygen supplied from the oxygen supply pipe 151 is controlled so that the amount of oxygen supplied to the oxygen supply pipe 151 does not influence the agitation of the zooplankton.

The zooplankton is actively active in the upper middle layer of the culture body 110 and is cultured under oxygen supply. In addition, the food supplied from the food supply unit 130 is scattered by the food, and the number of the animals is increased.

The sediments S such as excreta of zooplankton or food and body are settled in the lower part of the culture main body 110 and the sediment discharge pipe 117 is opened by the control unit 160 which senses the concentration of ammonia, ) To the outside. After keeping the temperature of the culture liquid, the oxygen supply amount, and the food supply amount constant, the zooplankton is cultured, and the zooplankton outlet 113 is opened by the same cycle as once or twice a week, .

As described above, the zooplankton culture apparatus according to the present invention can cultivate zooplankton at a high concentration by feeding food and oxygen, and adjusting the temperature of the culture liquid to a value suitable for culturing zooplankton.

In particular, it is possible to maximize the cultivation of zooplankton by supplying oxygen in a maximum amount that does not affect the activity of zooplankton when supplied.

In addition, it is possible to maintain the environment suitable for the culture of zooplankton by reducing the concentration of ammonia and the pH by periodically discharging the precipitates generated in the culture of zooplankton to the outside.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention as defined in the appended claims. You will know. Therefore, it is to be understood that the present invention is not limited to the above-described embodiments. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims. It is also to be understood that the invention includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention as defined by the appended claims.

100: zooplankton culture apparatus 110: culture body
111: Culture medium inlet tube 111a: Culture medium inlet valve
112: support frame 113: zooplankton outlet
115: overflow pipe 117: sediment discharge pipe
120: Culture medium supply part 130: Feeding part
131: food storage box 133: air pump
135: Feed stirrer 137: Feed input pipe
137a: feed inlet valve 140: underwater heater
141: power supply line 150:
151: oxygen supply pipe 160:

Claims (5)

In the zooplankton culture apparatus,
A culture body in which zooplankton and a culture liquid are accommodated;
A food supply unit provided at an upper portion of the culture body to supply food to the culture body;
An acid base for supplying oxygen into the culture body;
And an underwater heater disposed inside the culture medium of the culture medium to adjust the temperature of the culture medium,
Wherein the culture body comprises:
An animal plankton outlet disposed in the middle region of the whole height for discharging the cultured zooplankton to the outside;
And a culture liquid inlet disposed in the upper region for supplying the culture liquid to the inside,
And the acid part is disposed at a higher position than the zooplankton discharge pipe.
The method according to claim 1,
Wherein the underwater heater maintains the culture liquid at 24 ° C to 26 ° C.
3. The method of claim 2,
Wherein the food supply unit feeds the food at a daily dose of 0.84 ml / L · day to 0.92 ml / L · day.
The method of claim 3,
Wherein the acid part feeds oxygen at 4cc / Lday to 5.6cc / Lday.
5. The method according to any one of claims 1 to 4,
Wherein a lower portion of the culture body is formed so as to have a gradually smaller area, and a sediment discharge pipe for discharging the sediment contained in the culture liquid to the outside is formed.

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