KR20150125211A - Manufacturing method of honeyed ginseng having ginseng hydrolysis concentrate - Google Patents

Abstract

Disclosed is a method for producing a ginseng terminal fruit containing a ginseng hydrolyzed concentrate having high concentration IH-901 at high quality without loss of an IH-901 component. The method according to the present invention comprises: (a) a step of washing a wild ginseng; (b) a refining step of treating a core part and removing residual roots, so honey permeates in the washed wild ginseng; (c) a step of feeding the core part treated and refined wild ginseng in a sugared liquid having a sugar content of 60 brix% or more and including bee honey, black ginseng concentrate, frozen and dried royal jelly, ginseng hydrolyzed concentrate, and wild cultivated ginseng root concentrate; (d) a first sugaring step of steaming wild ginseng fed in the sugared liquid at 90-100°C for 0.5-2 hours; (e) a second sugaring step of steaming the firstly sugared wild ginseng at 70-90°C for 0.5-2 hours; (f) a third sugaring step of steaming the secondly sugared wild ginseng at 55-75°C for 100-200 hours; and (g) drying the thirdly sugared wild ginseng by hot air.

Description

Technical Field [0001] The present invention relates to a ginseng saponin containing a ginseng hydrolyzate,

More particularly, the present invention relates to a ginseng tablet containing a ginseng hydrolyzed concentrate and a method for producing the ginseng tablet.

Ginseng (Panax ginseng C.A. Meyer. Araliaceae) is one of the medicines traditionally used for the treatment of various diseases in Asian countries such as China, Korea, and Japan. In particular, ginsenoside (saponin), a major active ingredient of ginseng, has antioxidant activity, antioxidant activity, antioxidant activity in the central nervous system, cardiovascular system and immune system (Wu JY, et al., J. Immunol., 148: 1519 1999), anti-diabetic activity (Chang HM (1999), Lee, KS, Lee, KS, Lee, Et al., Biol. Pharm. Bull. 17: 635-9, 1994; Mochizuki M, et al., ≪ RTI ID = 0.0 > , Biol. Pharm. Bull. 18: 1197-1202, 1995).

Jung (果果) is a watery root, stem or fruit of sugar syrup and jujube for a long time to be boiled and chewy, sweet and refers to the Han. Traditionally, ginseng is made by taking ginseng for a while, removing the bitter taste, then adding water and sugar, and then sucking on a weak fire.

Recently, a variety of methods for manufacturing various ginseng confectioneries that have improved the conventional ginseng confection have been developed.

Patent Publication No. 0257732 discloses a method for preparing ginseng confection by mixing washed ginseng, adding sugar, boiling, and mixing honey. This method is a method of improving the condition .

In addition, Patent Publication No. 0471507 discloses a method of preparing ginseng juice by boiling ginseng, adding steamed ginseng and sugar, and infiltrating the ginseng with a low-pressure vacuum, followed by drying. And after the steeping, the molasses which has been melted is dipped in a sugar solution mixed with apple juice and sesame, and then dried to prepare a ginseng base.

Although various ginseng chews and preparation methods have been proposed in this way, they are limited to using sugar components or sesame seeds as a sugar solution.

On the other hand, it has been known that ginsenoside is metabolized by intestinal microorganisms after ingestion, and its metabolites also have various physiological activities (Karikura M, et al., Chem. Pharm. Bull, 39: 2357-61, 1991; M, et al., J. Tradit. Med. 11: 241-5, 1994; Akao T, et al., Biol. Pharm. Bull. 21: 245-9, 1998). For example, protopanaxadiol-based ginsenosides Rb1, Rb2, and Rc are produced by intestinal microorganisms of humans in the presence of IH-901 (20-O- beta -D-glucopyranosyl-20 (S) (Hasegawa H, et al., Planta Medica 63: 463-40, 1997; Tawab MA et al., Drug Metab. Dispos. 31: 1065-71, 2003), protopanaxatriol ginsenoside The side chains Re and Rg1 are metabolized to ginsenoside Rh1 or ginsenoside F1 (Hasegawa H, et al., Planta Medica 62: 453-7, 1996; Tawab MA, et al., Drug Metab. 1065-71, 2003), and the metabolites IH-901, Rh1 and F1 exhibit various physiological activities.

In particular, IH-901 is known to have anti-diabetes (Yun Suk Choi et al., J Ginseng Res. 31 (2): 79-85, 2007) and immunosuppressive action, (Wakabayashi C, et al., Oncol. Res. 9: 411-7, 1998, Lee SJ, et al., Cancer Lett. 144: 39 -43, 1999). For this reason, efforts have been extensively made to convert the ginsenosides into the above metabolites. Some researchers have attempted to produce genuine prosapogenin or sapogenin by chemical synthesis, weak hydrolysis and alkaline degradation (Han BH, et al., Planta Medica 44: 146-9, 1982; Chen Y, et al., Chem. Pharm. Bull. 35: 1653-5, 1987; Elyakov GB, et al., Synthesis of the ginseng glycosides and their analogs, Proc. 6th Int. Ginseng Symp. Seoul 74-83, 1993). However, since these methods cause various side reactions such as epimerization, hydration, hydroxylation and the like, recently, enzymes (Ko SR, et al., Biosci. Biotechnol. Biochem. Ko, SR, et al., Planta Med. 69: 285-6, 2003) and intestinal

Gentenoside in gentle conditions using bacteria (Hasegawa H, et al., Planta Medica 63: 463-40, 1997; Bae EA, et al., J. Microbial. Biotechnol. 13: 9-14, 2003) There are various methods of switching.

Methods using enzymes for the production of IH-901 from ginsenoside have been studied. Specifically, there are a method of producing IH-901 by hydrolyzing a diol-based ginsenoside with a Naringase enzyme, Was orally administered to mice and then decomposed in the large intestine to produce IH-901. However, this production method has a problem in that not only the yield of IH-901 is extremely low, but also various secondary metabolites are produced, resulting in production of high purity IH-901 (Karikura et al., Chem. Pharm. Bull. , 1990). Accordingly, a method for producing IH-901 by reacting beta-galactosidase enzyme with ginsenosides Rb1, Rb2, Rc, Rd, or a mixture thereof (Patent Document No. 2003-0094757) And a method for producing IH-901 by reacting a pectinase with purified ginsenoside (Patent Publication No. 0418604) has been developed, and a method for producing serylase or beta-galactosidase enzyme by reacting ginsenosides Rb1, Rb2, Rc, Rd or a mixture thereof to prepare IH-901 (Patent Publication No. 0377546).

This production method is not suitable for producing a fermented ginseng concentrate containing a large amount of IH-901 because the ginseng concentrate is used as a main raw material and each ginsenoside is separated and used. Has a problem that it is difficult to use the fermented product directly as a food, and the production amount of IH-901 is so small that its efficacy can not be expected. The applicant of the present invention has found that the combination of pectinase and beta-galactosidase under specific conditions is added to the ginseng extract or ginseng solution and fermented under specific conditions to obtain a high concentration of IH-901 The present inventors have proposed a fermented ginseng concentrate.

It is possible to consider the preparation of such a ginseng concentrate containing a high concentration of fermented ginseng concentrate containing IH-901, but this has not been described yet.

The present invention provides a method for producing a ginseng base containing a high concentration of IH-901-containing ginseng hydrolyzed concentrate with excellent quality without loss of IH-901 component.

In order to solve the above-described problems, the present invention provides a method for producing a ginseng, comprising: (a) washing ginseng; (b) a rectifying step for deep processing and removing fine roots to penetrate the washed ginseng; (c) adding the deep-processing and selected ginseng to a sugar solution containing honey, black ginseng concentrate, freeze-dried royal jelly, ginseng hydrolyzed concentrate and wild ginseng culture concentrate and having a sugar content of at least 60 brix%; (d) a first dipping step of steaming the ginseng added to the sugar solution at 90 to 100 DEG C for 0.5 to 2 hours; (e) steaming the first germinated raw ginseng at 70 to 90 DEG C for 0.5 to 2 hours; (f) a third digesting step of steaming the second germinated ginseng at 55 to 75 ° C for 100 to 200 hours; And (g) hot air-drying the third germinated ginseng, and a ginseng sap containing a ginseng hydrolyzed concentrate.

Also, the content of the sugar solution is preferably 0.2-5 parts by weight of the black ginseng concentrate, 0.2-5 parts by weight of the freeze-dried royal jelly, 0.1-2 parts by weight of the ginseng hydrolyzed concentrate and 0.1-2 parts by weight of the ginseng- 2 < / RTI > parts by weight.

Also, the ginseng hydrolyzed concentrate contains 5 to 50 mg / g of IH-901 (20-O-? -D-glucopyranosyl-20 (S) -protopanaxadiol).

Also, the ginseng hydrolyzed concentrate is obtained by mixing pectinase and beta-galactosidase in a ginseng extract prepared by adding water or an organic solvent, and then fermenting and concentrating the mixture.

Also, the hot air drying is performed until the moisture content of the ginseng becomes 15 ~ 25%.

According to the present invention, there is provided a method for manufacturing a high quality ginseng bean without loss of IH-901 component through a multistage gypsum process in which the sugar solution containing an appropriate amount of ginseng hydrolyzed concentrate is mixed at an optimum temperature and time, .

Also, it is possible to provide a ginseng root preparation and a manufacturing method which further comprises a black ginseng concentrate, a freeze-dried royal jelly and a wild ginseng culture root concentrate in the drench solution, but does not cause functional loss of the component.

Hereinafter, preferred embodiments of the present invention will be described in detail. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings. Throughout the specification, when an element is referred to as "including " an element, it means that it can include other elements, not excluding other elements, unless specifically stated otherwise.

The inventors of the present invention have found that, in order to produce a high quality ginseng preparation without loss of IH-901 component of a ginseng hydrolyzed concentrate when producing a ginseng hydrolyzate containing the ginseng hydrolyzed concentrate, It is necessary to carry out a multistage gypsum process to mature the ginseng by the time, and it is found that a high quality ginseng bean can be produced without functional loss of the component by further containing a specific acacia component and a trehalose concentrate in an optimal amount in the sugar solution, .

Accordingly, the present invention provides a method for preparing a ginseng, comprising: (a) washing ginseng; (b) a rectifying step for deep processing and removing fine roots to penetrate the washed ginseng; (c) adding the deep-processing and selected ginseng to a sugar solution containing honey, black ginseng concentrate, freeze-dried royal jelly, ginseng hydrolyzed concentrate and wild ginseng culture concentrate and having a sugar content of at least 60 brix%; (d) a first dipping step of steaming the ginseng added to the sugar solution at 90 to 100 DEG C for 0.5 to 2 hours; (e) steaming the first germinated raw ginseng at 70 to 90 DEG C for 0.5 to 2 hours; (f) a third digesting step of steaming the second germinated ginseng at 55 to 75 ° C for 100 to 200 hours; And (g) hot air-drying the third gangbang treated ginseng, and a process for producing ginseng ginseng containing ginseng hydrolyzed concentrate.

The steps (a) and (b) are a preliminary step for preparation and washing of fresh ginseng in a pretreatment process, and deep processing and removal of the root roots for penetration.

For example, raw ginseng raw materials can be used after confirming the origin and lotus root at the time of goods receipt and checking the items such as appearance, residual pesticide, heavy metals, and the like when they are put on the ginseng for 6 years. If the test can not be carried out directly, it may be used as a substitute for a certificate from the accredited laboratory.

For the washing of the fresh ginseng, after the inspection of heavy metals, ordinary bacteria, coliform group, etc. for the purified water for washing, the first washing is performed by immersing the ginseng in a water bottle containing the inspected purified water and the first washing ginseng is subjected to the second washing After finely removing the residue remaining in the ginseng with a brush or the like, and then performing third washing with a washing machine.

Deep processing can be performed by stabbing at a suitable interval (about 3 ~ 10 mm) using a needle to penetrate the washed ginseng well, and the ginseng root can be carried out by using a knife And then removing it.

The step (c) is a step of preparing the sugar solution and adding the selected ginseng to the sugar solution.

In the present invention, the sugar solution contains honey, black ginseng concentrate, freeze-dried royal jelly, ginseng hydrolyzed concentrate, and wild ginseng culture concentrate, and compared to a general sugar solution containing honey, black ginseng concentrate, freeze-dried royal jelly, A concentrate and a wild ginseng culture medium concentrate, and more particularly, a ginseng hydrolysis concentrate.

The black ginseng is steamed several times and the ginseng is turned black, and the active ingredient of ginseng does not flow out through the process of several times of boiling and drying, so that the content of active ingredient is higher than that of ginseng and red ginseng. The black ginseng concentrate used in the present invention can be obtained by concentrating such black gins by a known method. For example, black ginseng is prepared and extracted (from 80 to 100 ° C for 36 to 72 hours), filtered, concentrated (50 to 70 ° C, 50 to 70%), aging (60 to 96 hours at 10 to 30 ° C), and sterilization to enhance the content of ginsenoside Rg3 component.

The content of the black ginseng concentrate in the sugar solution of the present invention is not particularly limited, but may be preferably 0.2 to 5 parts by weight, more preferably 1 to 3 parts by weight based on 100 parts by weight of honey.

Royal jelly is a substance made by bees eating a lot of seeds and pollen, and is a nutrient containing protein, fat, carbohydrates and various trace elements. Royal jelly contains a lot of vitamins and rich nutrients. It is especially effective for the neurotransmitters of acetylcholine which enriches the skin of women and is effective for the health of muscles, bones and teeth. It is a parotin-like substance which is a salivary hormone that helps prevent aging. Is contained in a large amount. Royal jelly also contains 10-HAD, which inhibits the growth of cancer cells, and has been reported to have excellent effects on mental retardation, menopausal disorders, and blood pressure abnormalities. The freeze-dried royal jelly used in the present invention is a powdered royal jelly obtained by freeze-drying raw royal jelly by a known method.

The content of freeze-dried royal jelly in the sugar solution of the present invention is not particularly limited, but may be preferably 0.2 to 5 parts by weight, more preferably 1 to 3 parts by weight based on 100 parts by weight of honey.

The ginseng cultivar is a precise life sciences technique that cultivates wild ginseng in a medium similar to the growth conditions of natural ginseng such as inorganic salts and natural organic substances in organ, germ, and protoplasm of natural ginseng in aseptic state completely blocked from bacteria and virus As a result, the analysis of DNA structure and content of raw ginseng and wild ginseng cultivar showed that the cultivated ginseng and wild ginseng cultivar were 98.8% and contained various saponins not found in ginseng. It also contains various ginsenosides, various minerals and minerals that are present only in wild ginseng. The ginseng cultivar contains 40 mg or more of major saponin per 1 g of dry weight. The ginseng root is heated, cooked and dried to increase some of ginsenoside components. For example, the dried ginseng culturing roots are placed in an extraction tank, and then 70% ethanol aqueous solution is added at 10 times the weight of the ginseng culture root, and the mixture is extracted at 70 ° C for 10 hours. 30% ethanol And purified water, and concentrating and lyophilizing the extract until the solid content in the concentrate reaches 60% by weight or more.

In the sugar solution of the present invention, the content of the wild ginseng root concentrate is not particularly limited, but may be preferably 0.1 to 2 parts by weight, more preferably 0.2 to 0.6 parts by weight based on 100 parts by weight of honey.

The ginseng hydrolyzed concentrate is preferably 5 to 50 mg / g, more preferably 10 to 50 mg / g, still more preferably 20 to 50 mg / g as a raw material for producing a sugar solution containing a high content of IH-901. g, most preferably 30 to 50 mg / g of IH-901, may be used.

The ginseng hydrolyzed concentrate used in the present invention is prepared by mixing a pectinase and a beta-galactosidase in a ginseng extract prepared by adding water or an organic solvent to a raw material having a high content of IH-901, and then fermenting and concentrating And the like may be used.

Examples of the ginseng used in the production of the ginseng hydrolyzed concentrate may include ginseng, white ginseng, ginseng, dried ginseng, red ginseng, taegeuk ginseng, extracts thereof and powders thereof. The organic solvent used for preparing the ginseng extract may be, for example, acetonitrile, di Dimethyl sulfoxide, methanol, ethanol, 1-propanol, 2-propanol, and the like can be used. The ginseng extract may be prepared by adding water or an organic solvent to ginseng and then mixing it with water and an organic solvent appropriately. The use of a mixture of organic solvents can increase the solubility of the intermediate reactant produced by the enzymatic reaction and ultimately improve the content of IH-901 in the ginseng hydrolyzed concentrate. The mixture of pectinase and beta-galactosidase in the ginseng extract thus prepared can be carried out in a ratio of 1: 1 to 100 of pectinase and beta-galactosidase, and 1 To 50% by weight. IH-901 content of the final ginseng hydrolyzed concentrate can be further increased when pectinase and beta-galactosidase are mixed together, as compared with the case where pectinase or beta-galactosidase is separately mixed. The fermentation of ginseng extract mixed with pectinase and beta-galactosidase can be carried out, for example, by adding pectinase and beta-galactosidase to ginseng extract, adding the mixture at pH 3 to 8 and 10 to 70 ° C for 1 to 72 hours ≪ / RTI > during fermentation. Thereafter, the fermented ginseng extract may be directly concentrated or centrifuged to remove the supernatant, and the concentrate may be carried out using a commonly used vacuum decompression concentrator.

The content of the ginseng hydrolyzed concentrate in the sugar solution of the present invention is not particularly limited, but is preferably 0.1 to 2 parts by weight, more preferably 0.5 to 1 part by weight, based on 100 parts by weight of honey.

The sugar solution can be prepared by mixing the sugar components of the above sugars and adding purified water to the sugar content to be at least 60brix%, preferably at least 70brix%.

The prepared dipping solution is prepared, and the selected ginseng is put into a sugarbath, and then the dripping is performed as in steps (d) to (f).

In the present invention, a multistage gypsum process is carried out in order to avoid loss of IH-901 component during glycyrrhiza using a sugar solution containing a proper amount of ginseng hydrolyzed concentrate.

(D) a first liquor which steams the ginseng at 90 to 100 ° C for 0.5 to 2 hours, (e) a second liquor which steams the first grated raw ginseng at 70 to 90 ° C for 0.5 to 2 hours, And (f) a third step of gluing the second ganged ginseng at 55 to 75 ° C for 100 to 200 hours to obtain a ginseng base having little loss of IH-901 component . At this time, preferably, the first intestine is mixed at 93 to 97 ° C for 0.8 to 1.2 hours, the second intestine is mixed at 78 to 82 ° C for 0.8 to 1.2 hours, and the third intestine is mixed at 130 to 63 For ~ 150 hours.

The step (g) is a step of hot-air drying the fresh ginseng which has been dripped to the finishing step.

For example, in the hot-air drying, the ginseng which has been finished with the ginger is taken out and dried at 50 to 55 ° C in a hot-air drier. While the drying state is checked during drying, the moisture content is adjusted to 15 to 25%, preferably 17 to 21% .

The content ratio of the ginseng and the sugar solution in the dried ginseng base may be 1: 0.1 to 1: 0.6, preferably 1: 0.2 to 1: 0.5, and most preferably 1: 0.3 to 1: 0.4 on a weight basis.

The finished ginseng juice is packaged and prepared as a product. Special attention should be paid to product contamination during packaging. Before the start of packaging work, sanitary conditions of the workshop and workers should be checked and disinfected. In the case of packing materials, the markings and defects must be checked in advance and used according to the specifications. In addition, the hygienic hygienic condition of the products and hygienic products that have been dried thoroughly is to put the shape of the jug into the vacuum packaging film properly. At this time, the hands, clothes and clothes of the work table and workers should be disinfected thoroughly with alcohol so as not to contaminate microorganisms such as E. coli. Be careful. Vacuum packaged products are spread evenly in a hot air dryer and dry sterilized at 80 ~ 85 ℃ for about 1 hour. After sterilized products are inspected for the final product, if there is no abnormality, insert product manual and quality guarantee Finish after wrapping.

Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples.

Example

After 6 years of domestic ginseng preparation, it was cleaned, deep-processed and selected, and then 100 parts by weight of honey (domestic product, sundried honey), 2 parts by weight of black ginseng concentrate (domestic component, 60 to 70% of solid content), 2 parts by weight of freeze-dried royal jelly, 0.8 parts by weight of a hydrolyzed concentrate (IH-901 36 mg / g, manufactured by IKA CORPORATION) and 0.4 part by weight of concentrated ginseng culture medium (60-70% solids content) (1 st glycyrrhiza) at 94-96 ° C for 1 hour, steamed for 1 hour at 79-81 ° C (second lanceolate) and steamed for 140 hours at 64-66 ° C (third lanceolate) Dangling was performed. The dried ginseng was dried to a moisture content of 18 to 20% in a hot air drier at 50 to 55 ° C to prepare a ginseng base. In the final dried ginseng root, the ginseng content was 74.72% by weight and the sugar solution content was 25.28% by weight.

Comparative Example  One

The ginseng base was prepared in the same manner as in Example except that only the first intestine and the third intestine were performed without performing the second intestine.

Comparative Example  2

The ginseng base was prepared in the same manner as in Example except that only the second intestine and the third intestine were performed without performing the first intestine.

Comparative Example  3

Ginseng juice was prepared in the same manner as in Example except that only the third intestine was performed without performing the first intestine and the second intestine.

Experimental Example

In order to comparatively analyze the content of IH-901 in the ginseng base prepared according to Examples and Comparative Examples, 1 g of ginseng base (based on 100 g) was extracted three times with 60 ml of water saturated butanol, and then the butanol fraction was concentrated 120 mg of the concentrate was dissolved in methanol, and the content of IH-901 was analyzed by high pressure liquid chromatography. The results are shown in Table 1 below.

As shown in Table 1, when a multistage gypsum process was performed at an optimum temperature and time in a sugar solution containing a proper amount of ginseng hydrolyzed concentrate according to the present invention, IH-901 produced ginseng juice having little ingredient loss (IH-901 content: 7.2 mg) was added to the final ginseng base, and the content of IH-901 in the final ginseng base was 7.1 mg. On the other hand, it is found that the IH-901 component is excessively lost when the dripping process is performed by changing from the example.

The preferred embodiments of the present invention have been described in detail above. It will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Accordingly, the scope of the present invention is defined by the appended claims rather than the foregoing detailed description, and all changes or modifications derived from the meaning, range, and equivalence of the claims are included in the scope of the present invention Should be interpreted.

Claims (5)

(a) washing the ginseng;
(b) a rectifying step for deep processing and removing fine roots to penetrate the washed ginseng;
(c) adding the deep-processing and selected ginseng to a sugar solution containing honey, black ginseng concentrate, freeze-dried royal jelly, ginseng hydrolyzed concentrate and wild ginseng culture concentrate and having a sugar content of at least 60 brix%;
(d) a first dipping step of steaming the ginseng added to the sugar solution at 90 to 100 DEG C for 0.5 to 2 hours;
(e) steaming the first germinated raw ginseng at 70 to 90 DEG C for 0.5 to 2 hours;
(f) a third digesting step of steaming the second germinated ginseng at 55 to 75 ° C for 100 to 200 hours; And
(g) hot-air drying the third gredicated ginseng;
Containing ginseng hydrolyzed concentrate and a method for producing the same. The method according to claim 1,
Wherein the sugar solution content is 0.2 to 5 parts by weight of the black ginseng concentrate, 0.2 to 5 parts by weight of the freeze-dried royal jelly, 0.1 to 2 parts by weight of the ginseng hydrolyzed concentrate and 0.1 to 2 parts by weight of the ginseng root concentrate, By weight. The method according to claim 1,
Wherein the ginseng hydrolyzed concentrate contains 5 to 50 mg / g of IH-901 (20-O-? -D-glucopyranosyl-20 (S) -protopanaxadiol). The method of claim 3,
Wherein the ginseng hydrolyzed concentrate is a mixture of pectinase and beta-galactosidase in a ginseng extract prepared by adding water or an organic solvent, followed by fermentation and concentration. The method according to claim 1,
Wherein the hot air drying is performed until the moisture content of the ginseng becomes 15 to 25%.